LOCUS       DALTZZ010000001         3618 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_439,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000001 DALTZZ010000000
VERSION     DALTZZ010000001.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1338
                     /locus_tag="O0X12_00005"
     CDS             <1..1338
                     /locus_tag="O0X12_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HJK52579.1"
                     /translation="SGKTWVLKQFGNDYFSDTAYFNFEETSALQTIFKTECDPCCILT
                     ELGRIRGKPIIPGTTLLILDEIQFSLDALFSLKDFTEKLPEQHIACADSLLYETIENN
                     LSFLTEQVYIVTLHPLNFGEFLRANNKETLITFLDDSVPLLPLPEPVTTELNRLCQEY
                     FITGGMPEAVSDWITHHDCNRVEEIHRRILQDYLHDISIYAPKKERVKMYDIIYAIPN
                     QLAKKNRRFMLSYLKKGAETKDYEKAVWWLAKKAGIVHKVERLTRPGIPLEACLNHSN
                     FKLYFADVGLLRTLAGITAEEILTKTDNPSEIRKALTENFVLTELLSRNMFRVYYWKY
                     DWREGEVDFVVKIGKYTIPFEVNNKIHLWPKALIRYQELYLPDIILKISHDVPQYYSS
                     EAPLKFPLYFVWRMPEFIISIITLRDLILPPEERQNTIYGRQFESASGVPARR"
     gene            complement(1416..1955)
                     /locus_tag="O0X12_00010"
     CDS             complement(1416..1955)
                     /locus_tag="O0X12_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HJK52580.1"
                     /translation="MEFSSALLTAAELMAVSARTAPKSKGSDAIEIKVLGPDELEPLA
                     AEMEEIAASSGMKFFLRDAGNIRQSGACVLIGADGLSTLGLNCGACGHATCAEMKASV
                     DIAPVDSLYKGPVCALRSADLGIAVGSAVKTASILNVDNRIMFSAGTAALALGLLPHS
                     TIAFGIPLSVTGKSPYFDR"
     gene            complement(2069..2986)
                     /locus_tag="O0X12_00015"
     CDS             complement(2069..2986)
                     /locus_tag="O0X12_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HJK52581.1"
                     /translation="MATLKRTFQIWIPRYLSSIETKNYSENTIEAYGRVLKLFARYKT
                     YLAEHDGDTPENTEALAGIGMSADVDADALEITDFFTMIRNERHLKPASLHQYDSALS
                     SFYRHLIAQNVVDVNPMSRVERPKIKDRELKYLRHKEVMAFIASLTNPRDALLIRTIY
                     ATGMRVSEICGLCAEHIDFEEQTIRVRGKGGKIRIVFCDPETLSQIREHLGGRSEGPV
                     FLGNRGKAISPRTVQHIFQLYAPPGITPHKIRHSYASELYKRSHNLRVVQENLGHNSI
                     QTTEIYIHTDLDERRRAYQEYFPLATGGE"
BASE COUNT         1051 a          985 c          789 g          793 t
ORIGIN      
        1 tccggcaaaa catgggttct caagcagttt ggaaacgatt acttttctga taccgcctac
       61 ttcaactttg aagaaacttc ggctctccaa accatcttca agactgagtg cgacccttgc
      121 tgcatcctga ctgaacttgg tcgcatcaga ggaaaaccca ttatcccggg aacaaccctc
      181 ctcattctgg atgagataca gttcagtctg gatgccctat tctccctcaa agacttcacg
      241 gaaaaactcc ccgaacagca tatcgcctgt gcagactccc tattatatga aactattgaa
      301 aataaccttt catttctcac tgaacaagtc tatatagtaa ccctccaccc cctgaacttc
      361 ggggagttcc tgcgtgcaaa caataaagaa actctcatca cgttccttga cgactctgtt
      421 ccgttgctgc cgctgccgga acccgtcacc accgagctta accggctgtg tcaagagtac
      481 ttcatcaccg gtggaatgcc agaagctgtc agtgactgga taacgcatca cgactgtaac
      541 cgcgttgaag agatccatag acggattctc caagattatc tccatgatat cagcatctat
      601 gcaccaaaaa aagagagagt caagatgtat gacatcatat acgcaatccc aaatcagctt
      661 gcaaagaaga accgcagatt tatgttaagt tatctaaaga agggagcaga gacaaaggat
      721 tatgagaagg ccgtgtggtg gctcgccaag aaagcaggca tagtccacaa agtggagagg
      781 ctgactaggc caggaattcc tcttgaggcc tgtctcaatc attccaactt caagctctac
      841 ttcgcggatg tcgggcttct ccgtacatta gcagggatca ccgcagaaga gattctcacc
      901 aagaccgaca atccttccga gatccgcaaa gcacttaccg aaaactttgt tcttaccgag
      961 ctcctgtccc ggaatatgtt ccgtgtctat tactggaagt acgactggag ggagggagag
     1021 gttgattttg tagttaaaat aggtaaatac actatacctt ttgaggtaaa taacaaaata
     1081 cacctctggc caaaggctct aatcagatat caggaactct acctgcccga tattatactg
     1141 aagatcagtc atgacgtccc gcaatactat tcatccgaag ctcccctgaa atttcctctg
     1201 tattttgtct ggaggatgcc ggagtttatt atatcaataa tcacattaag ggatctaatc
     1261 ctaccgccag aggagagaca aaacacgatt tatgggagac aatttgaatc agcatctgga
     1321 gtacctgcaa gaagatgacc ggaaccaaat caccgccctc tctgtaacaa ctaccagcat
     1381 aaacaggaaa aaaaataaaa tcagccccaa agtcctcatc gatcaaagta tggactcttc
     1441 cccgtcaccg acaacggaat accaaacgca atcgtcgaat gcggcaaaag ccccagggcc
     1501 agcgccgcag tccccgcaga aaacataatc cggttatcca cattcagaat cgaggcagtc
     1561 ttcaccgccg atcccaccgc aatacccaga tcagccgacc gcagagcaca caccggaccc
     1621 ttatacaacg aatccaccgg agcaatatca acagacgcct tcatctccgc acacgtcgca
     1681 tgaccgcacg ccccgcagtt cagaccaagc gttgacaaac catccgcccc gatcagaaca
     1741 catgccccgc tctgccggat attccccgca tcacggagaa aaaacttcat cccggacgaa
     1801 gcagcaatct cctccatctc cgcggcaagc ggctcaagct catccggacc gagaacctta
     1861 atctcaatcg catcagaacc cttggacttc ggcgcagtcc tggcagaaac tgccatgagc
     1921 tctgcagcag tgagaagtgc tgaagaaaat tccatgctct tctctatagc cctcacccgt
     1981 tatcattctt ctgatcggat tcaaaaaaat agatatagca ttattcggtt tattccatac
     2041 gcagcagaac ctgttcgaat aaacaggatc actccccgcc ggttgccagc gggaaatact
     2101 cctgatatgc tctcctgcgt tcatcaagat cggtatggat atagatctcc gtcgtctgaa
     2161 tcgaattatg gccgagattc tcctgcacaa cacggagatt atgcgaacgc ttgtacaact
     2221 cactcgcata actgtgccgg atcttatgcg gcgtaatccc cggaggagca tacaactgaa
     2281 aaatatgctg cacagtccgg ggagagatcg cctttccccg gttcccgaga aaaaccggac
     2341 cctccgatct ccccccaaga tgctcccgaa tctgggaaag cgtctcagga tcgcagaaca
     2401 caatccggat cttccctccc ttaccccgca cgcgaattgt ctgctcctca aaatcaatat
     2461 gttccgcaca cagaccgcaa atctctgaaa cacgcatacc cgttgcataa atcgtccgga
     2521 tcagcagagc gtcacgtgga ttggtaagcg acgcaatgaa tgccatcacc tccttgtgcc
     2581 gcagatactt cagctcgcga tccttgatct tgggccgctc aacacggctc atgggattga
     2641 catcaaccac gttctgggca atcaggtgcc ggtaaaagga gctcaaagcc gaatcatact
     2701 gatgcagact tgccggtttt aagtgacgtt cgttgcggat catcgtaaaa aaatccgtaa
     2761 tctcaagcgc atccgcatcc acatccgcac tcatcccaat gcccgcaaga gcctccgtgt
     2821 tttccggagt atcaccgtca tgctcggcaa gataggtttt gtagcgggca aacagtttga
     2881 gaactctgcc gtacgcctcg atggtatttt ccgagtagtt cttcgtctcg atgctgctga
     2941 ggtagcgggg gatccagatc tggaaagttc gtttcagcgt ggccatgttg cagtcgaaga
     3001 gtaattgtat ctctgagaag ataaggatta cgcataatag acattatgcg caatattttg
     3061 ggggttttgg aggtttgaga ataaggagaa ttaagcgata aaatgaaaaa ataagcctga
     3121 ataacagatt ttcacaaaac atactgatca ccccgaagaa gagatcagat cccggaggtc
     3181 ctgcccggga cgaaaatagg atcatgaaaa atccggggtt tgagggcagg gacaaacagg
     3241 gaggatgaat cacccgaccc tgccggcgaa gtccgctcaa acgccgtcta tgaggatcgg
     3301 tttttcggaa ggttccgtgc aggtccggca gaaccatccg tgcgtgagga ttgcacacca
     3361 ttgcgtacat ggatcttctg agactgccga aagagaatct gccaacagaa cggatctgcg
     3421 gtacaccacc gcagaccaaa accacaggca gtaacacagg aaaagtaccc gccactcagg
     3481 gaacagagat catgcacaac aaaccaaaca gaaaacactc ccggatgcag ccggcaaaat
     3541 catccctcac accttcagta ccagtatcgc gtgggattca gacatgggaa tttggaacag
     3601 cataaaatac agctgaga
//
LOCUS       DALTZZ010000002         2906 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_662,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000002 DALTZZ010000000
VERSION     DALTZZ010000002.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2906)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2906)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2906
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(169..1041)
                     /gene="modA"
                     /locus_tag="O0X12_00020"
     CDS             complement(169..1041)
                     /gene="modA"
                     /locus_tag="O0X12_00020"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HJK52582.1"
                     /translation="MVWISMKLTKTWIFAALLVCVVLTMGMAGCIGTENPATPTPTPT
                     QTGTTSDLSGEINVFAAASLTGVLNELGEQFEAQHENVEVHFNFAGSQTLKTQILEGA
                     DVDMYISANNAQFTPVVEAGLISNPKVLLKNKLAIVVPKENNKQITDLAGMAEEGVVL
                     VIGNKDVPFGQYTRDIINKYQNDSHPGYVDAFMANVVSEVDAVDKIKPLLILDEADAS
                     LVYKSDISKSDKEDITLIEIPDEYNVIADYPYGIIDDTKNRALVEAWEAYLTGPEGTA
                     LLLDYGFDPVSATA"
     gene            complement(1161..2126)
                     /locus_tag="O0X12_00025"
     CDS             complement(1161..2126)
                     /locus_tag="O0X12_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HJK52583.1"
                     /translation="MAKVTIIGATGQVGSYAAHAVSQFPHVQEMCLYGRPGNEQYLDG
                     LAHDMMDSFAARGTNTRVTFGTNPKELRGSDIIVLTAGVPRKPGQTRLDLALENAKIV
                     RHYAEQVGRMAPGSILLVVTNPVDIMTTVALKYSGMMPHRVFGLGTHLDSMRLKACLA
                     EFFNVHVSEVHTRIIGEHGDTMVPMWSATTIGGIQIDNMIGIAKLPREQMIERVKNSG
                     SYIIQAKGATVFGPGDAIATLIRTIVEDENRMLTVSTQIRREIFGHEGVCISVPARIT
                     RGGVFPIGVRLSITETALFGESVKLIKEMTERVCAALDAESQSSE"
     gene            complement(2149..2460)
                     /gene="ffs"
                     /locus_tag="O0X12_00030"
     ncRNA           complement(2149..2460)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="O0X12_00030"
                     /product="signal recognition particle sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01857"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="archaeal-type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01857"
     gene            complement(2517..2588)
                     /locus_tag="O0X12_00035"
     tRNA            complement(2517..2588)
                     /locus_tag="O0X12_00035"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2554..2556),aa:Ile,seq:cat)
     gene            complement(2774..2846)
                     /locus_tag="O0X12_00040"
     tRNA            complement(2774..2846)
                     /locus_tag="O0X12_00040"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2811..2813),aa:Asn,seq:gtt)
BASE COUNT          815 a          890 c          607 g          594 t
ORIGIN      
        1 atcctcaaca ctcacccgcg taaaaagcga aagcagtgga atcgtgatga aaacaaggaa
       61 caggaggatg agcccccccg ccaccaccat cgtgccaatg cgggtagcag aatgtcttcg
      121 tgtgtcggtt gaactcatac tttcctgtaa acgtatggca acagaagatt atgcagttgc
      181 cgaaacgggg tcaaacccgt agtcaagcag aagtgcagta ccctcaggtc cggtcagata
      241 agcctcccag gcctcaacca gtgcacggtt cttggtgtca tcaataatac cgtacggata
      301 gtcggcaata acattgtact catccggaat ctcgattaac gtaatatcct ccttatcgga
      361 cttggagata tcagacttgt acacaaggga agcatccgcc tcatcaagaa taagcagcgg
      421 cttaatctta tcaactgcat caacctcgga cactacattt gccataaacg catcaacata
      481 gcccgggtgg ctgtcattct gatacttgtt gataatatca cgggtgtact gaccaaacgg
      541 cacatcctta ttaccgatca caagcacaac accctcctct gccataccgg caagatccgt
      601 aatctgcttg ttgttttcct tcgggacaac aattgcaagc ttgttcttta acagaacctt
      661 cggattggaa atcagaccgg cctcaacaac cggcgtaaac tgggcattgt ttgcggagat
      721 gtacatatcc acatcagcac cctcaagaat ctgcgtcttc agcgtctgac tgccggcaaa
      781 gttgaaatga acctccacat tctcatgctg tgcctcaaac tgctcgccca gctcgttcag
      841 aacaccggtc agagaagcag cagcaaacac attgatctca ccggaaagat cagaagtggt
      901 tccggtctgc gtcggggtcg gggtaggagt tgccgggttt tcagtaccga tacacccagc
      961 catacccatc gtcagcacta cacacacaag cagtgctgca aaaatccagg tcttggtcag
     1021 cttcatacta atccatacca tatttgactg aatcgtattt aagagtaagc tgattgccat
     1081 taacatgtaa acttaacgta gttaacaaaa acatcagaca gcaccccgcc ccacaccaac
     1141 aggcaaacca aaagagaaaa ttactcagac gactgtgact ccgcgtccag cgcagcacaa
     1201 accctctctg tcatctcctt aatcagcttc accgactccc caaacagcgc cgtctccgta
     1261 atcgacagac gaaccccgat cggaaacacc ccgccgcgcg taatccgtgc cggaacacta
     1321 atacagacac cctcatgccc gaaaatctcg cggcgaatct gcgtcgaaac cgtcagcatc
     1381 cggttctcat cctcaacaat cgtccggata agcgtcgcaa tcgcatcgcc cggcccgaac
     1441 accgtcgcac ccttcgcctg aataatatac gaaccgctgt ttttcacccg ctcaatcatc
     1501 tgctcgcgcg gaagcttcgc aatcccgatc atattatcga tctgaatacc gccgatcgtc
     1561 gtcgccgacc acatcggcac catcgtatcc ccgtgctcgc cgataatccg cgtgtgaacc
     1621 tcactcacat gcacattaaa aaactccgca agacacgcct tcagacgcat cgaatccagg
     1681 tgcgtaccaa gaccaaacac ccggtgcggc atcatccccg agtacttcag ggcaaccgtc
     1741 gtcataatat cgacaggatt tgtcaccacc aaaagaatcg aacccggcgc catccgcccc
     1801 acctgctccg cataatgccg gacaatcttc gcattctcca gcgcaagatc cagccgcgtc
     1861 tgaccaggct ttcgcggaac acccgccgta agcacaataa tatccgaacc gcgcagctcc
     1921 ttgggattcg tcccgaacgt cacccgcgta ttcgtacccc gtgcagcaaa cgagtccatc
     1981 atgtcatgag caagaccatc cagatactgc tcattccccg gccgtccgta caaacacatc
     2041 tcctgcacat ggggaaactg ggaaacagca tgcgctgcat acgaaccaac ctgaccggtc
     2101 gcaccgatga tcgtcacttt tgccataaac aaaccctcgc aaaatcaaga tgggacacgt
     2161 tctcggtaga aagcgcacat ccagcctctc tgacataccc cttctccgcc ccgcaaccct
     2221 gtgggcgcca tgcgttcacg gtaggtctgc ccccttccgg gcctgaaccg gtgcccgcaa
     2281 caagagggaa acacatccct ccggatatgt ccgccctcgc cgctgacctc acaggacgag
     2341 gaatcaacgt cagaatatgc cgcgtcagtt ggactgccac agccgtcctc aaacttcgtc
     2401 ccctgcgtat tggctatttc aggtaacagg tgacgccaag ccacccggac tagtcccata
     2461 tataatgagt cattcactgg ttaaaatgat gtaggatgca cgccccaaaa caaaaatggg
     2521 cccggagggg ttcgaaccca cgaccacccg gttatgagcc gggcgctcca ccactaagct
     2581 acaggcccga tactacgaga ataagtacgg aaaatgggat tacagaggat tctgcatccc
     2641 gaaagaaatt ttgttccctt gcagaagaac atatcatatt tggcatactc atacttaaag
     2701 gtgtccttct gcggacaaaa aataacaaaa aaacatcgta ttcagaaaaa tcagaaacaa
     2761 aaaacaaaaa cagcgccgcc aacaggactc gaacctgtga catgctggtt aacagccagc
     2821 cactctacca actgagttat ggcggcaatc aaacccgttg cgaacaaaca tcgcacgtgt
     2881 cttgcctaaa tagatgtgct atgcac
//
LOCUS       DALTZZ010000003         3511 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_925,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000003 DALTZZ010000000
VERSION     DALTZZ010000003.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1622
                     /locus_tag="O0X12_00045"
     CDS             <1..1622
                     /locus_tag="O0X12_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HJK52584.1"
                     /translation="IYQSNGQIDTRHGKLILAILICQDIAVIPIMLLLPVIAGVGGDM
                     MSSVMSLGTSLVALVVVLGVALVVVPRILDVVASRRNRELFIISVMTICIGVAWILSL
                     AGVSSSLGAFLAGVAIAGSEYNHEVLGIIIPIRDLMTSVFFISVGMMLSVVFMLENIL
                     FILAIVAVLFVGKMVITSAAGLLAGASTGAAVIGGASLAHIGEFSFVLGTTGMSLGLM
                     SDTIYQVFLAVAILSMAMAPFAVEAAPKIARHVVCSPLFGKGCPPGTTDQVMPTDVSA
                     DEDHEYVIVVGFGPAGKYVVRALKRVGREYIILEMNPETVAAERAKGENIAYGDASLE
                     PILRYAGIERAKTLVISIPDSATVRGIVSTARRMNPRTTIITRTRYTGESPTLFKIGV
                     DEVIADEREAGLALTRRVFASENVPAKDLDQYVREVRAEIFSEREEAMAQKAEESRLR
                     RISATLEPVERTEFIHVPVHPDSEAAGKTLADLHLRKQYHVSVMSLRRESGEMVMPDG
                     TTVVLAHDMVLLSGSREDVAAVQRLFGKKQG"
     gene            1794..2402
                     /locus_tag="O0X12_00050"
     CDS             1794..2402
                     /locus_tag="O0X12_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HJK52585.1"
                     /translation="MAPKKKQKDLLKLFSDITQKTKIVEPMKQLHGTLRDQDAVEREI
                     ALIMREIQERGFFKTKLEPIQLARLVTAFHAGKNDTEIARELGDEKLSKTVARARVRL
                     KLFRDLDFNMPFDQEQMLTLMSTDKTMREISEELGISPTTLREYRHVIEQKEDTTLDP
                     YLERIRDVMEDRDLTEKMASGLQEDTLGEAIDISESDSIEIN"
     gene            2472..3134
                     /locus_tag="O0X12_00055"
     CDS             2472..3134
                     /locus_tag="O0X12_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HJK52586.1"
                     /translation="MYIRYVGHSCFSLEGSRKVLIDPMPEAAASVDADLVLVTHAHGD
                     HLGITVDLKKQTVAIHELANYLSRKGVPAVGMNIGGTYAADGVKVTMVPALHSSSVTE
                     NGVDLYMGSAAGFVVEMDGIVVYHAGDTGLFSDMKLIHDLYHPDVALLPVGGHYTMGP
                     AEAMIAAEWVGAPLVIPMHYNTFPAIAQDLTEFEHAIESTTSMLVDVVAPGDGVEVSP
                     QQ"
     gene            3250..>3511
                     /locus_tag="O0X12_00060"
     CDS             3250..>3511
                     /locus_tag="O0X12_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HJK52587.1"
                     /translation="MVSLESVFHLKAEKTTVPREVIAGLTTFFAMAYIIIVNPAILSE
                     TGMEWGAVFLATIIAAIAGTLIIGLYANVPFAQAPGMGLNTFF"
BASE COUNT          694 a          727 c         1158 g          932 t
ORIGIN      
        1 acatttatca gagcaacggc cagatcgaca cccgccacgg aaagctgatt ctggcgattc
       61 tcatctgtca ggacattgcg gttatcccca tcatgctgct gcttccggtg attgcgggcg
      121 ttggcggcga tatgatgtcg tcggtgatgt ctttgggcac aagtcttgtg gccctggttg
      181 tggtgcttgg ggttgcgctg gttgtggttc cccggattct tgacgtggtt gcatcccgca
      241 ggaaccggga acttttcatc atctcggtta tgacgatctg tatcggggtt gcttggattc
      301 tgtcgcttgc cggagtttcc tcgtcgcttg gtgcgttcct tgcgggtgtg gcgattgcgg
      361 ggtcggagta caaccacgag gttctgggaa ttattatacc tatccgggat ctgatgacga
      421 gtgtgttttt catctcggtc ggtatgatgc tgtcggttgt gtttatgctg gagaatattc
      481 tgtttattct tgcgatcgtt gcagtgctgt ttgtgggtaa gatggtgatt acgagtgctg
      541 cgggtctgct tgcgggagcc tcaacggggg ctgcggttat cggcggtgca agccttgccc
      601 atatcggtga gttttcgttt gtgctcggaa caaccggtat gtcgctcggg ctgatgagtg
      661 atacgatcta tcaggtgttt ctggcggttg cgattctgtc gatggcaatg gcgccgtttg
      721 cggtggaggc cgcaccgaag atcgcacggc atgtggtctg ttcgccgctg ttcggcaagg
      781 gctgccctcc cggtacaact gaccaggtga tgcctacgga cgtgtccgcg gatgaggatc
      841 atgagtatgt gattgtggtc gggttcggtc ctgcgggaaa gtacgtggtt cgggcactga
      901 aacgggtggg ccgtgagtat attattcttg agatgaaccc ggagacggtt gcagccgagc
      961 gggcgaaagg agagaatatt gcctacggtg atgcgtcgct tgagccgatt ctgcggtatg
     1021 cgggaattga gcgggcgaag acgctcgtga tctcgattcc cgactcggca acggtccggg
     1081 gcattgtgtc aaccgcccgg cggatgaatc cgcggacaac aatcattacg cggacccggt
     1141 acacagggga gagtccgact ctgtttaaga tcggggtgga tgaggttatt gctgatgagc
     1201 gtgaggcagg tcttgccctg acgcggcggg tgtttgcaag tgagaatgtg ccggcaaagg
     1261 atctggatca gtatgtgcgt gaggtgcgtg cggagatctt ttcggaacgt gaggaagcaa
     1321 tggcacagaa agcggaagaa agccgtctgc ggaggatttc ggcaaccctt gaaccggtgg
     1381 agcggacgga gtttattcat gtgccggtac atccggattc ggaggccgca ggaaaaacgc
     1441 tggcggatct gcatctgaga aaacagtatc atgtgtcggt gatgtcgctc cggcgggagt
     1501 cgggcgagat ggtgatgccg gacgggacaa ctgtggtgct tgcgcatgat atggtgctgc
     1561 tgtcgggaag ccgcgaggat gttgcggcgg tgcagcgttt gtttgggaaa aagcagggat
     1621 aattttcgtg ggttttttcc cgcatatatt tttggggata atcatacagt cgttgtggta
     1681 attaatgttt tgcatctttt ccgggagttt tcagagcgcc agggtgcatc cagaatgatt
     1741 atatgctttg aagtacattt tcatcgaata acccgtttgg gaggaatatg aatatggccc
     1801 caaagaagaa acagaaggat ttactgaagc tgttttctga tattacgcag aaaacaaaga
     1861 ttgtggagcc gatgaagcag ctccacggca cgcttcgtga tcaggatgcg gtcgagcgtg
     1921 agatcgccct gattatgcgt gagattcagg agcgtggttt ctttaagacg aagctggagc
     1981 cgattcagct tgcgcgtctg gttacggcgt ttcatgcagg gaagaatgat acggagatcg
     2041 cccgtgagct tggagatgag aagctttcca agacggttgc ccgtgcacgg gtgcggctga
     2101 agctgttccg cgatcttgat tttaatatgc cgtttgatca ggagcagatg ctgacgctga
     2161 tgagtacgga taagacaatg cgggagatct ccgaggagct gggtatcagt ccgacaacgc
     2221 tgcgggagta ccggcacgtg attgagcaga aggaggatac gacgctggat ccgtatctgg
     2281 aacgtatccg cgatgtgatg gaggaccggg atctgaccga gaagatggcg tcgggtctgc
     2341 aggaggatac gctcggggag gctattgata tctccgagtc ggatagtatt gagatcaact
     2401 gatttccctc ccgttttttc tgatatttcc ccgacccatg tttatttata ggtaggaggg
     2461 ggagtactgt tatgtatatc cgctatgtgg gacattcctg tttttcactg gagggctccc
     2521 gaaaggtttt gatcgatccg atgccggagg ctgctgcttc ggttgatgcg gatctggttc
     2581 tggtgacgca tgctcacgga gatcatctgg gtattacggt ggatctcaaa aagcagacgg
     2641 tggcaattca tgagcttgcg aattatctgt cgcggaaagg tgttcctgcg gttgggatga
     2701 acatcggcgg aacctatgcg gcggacgggg tgaaggtgac gatggttccg gctctgcatt
     2761 cgtcgtcggt gacggagaac ggggtggatc tgtatatggg gtctgctgcc gggtttgtgg
     2821 tggagatgga cgggattgtt gtgtatcatg cgggtgatac gggactgttt tcggatatga
     2881 agctgatcca tgatctgtat catccggatg ttgctctgct gccggttggg ggtcattata
     2941 cgatggggcc tgccgaggcg atgattgcgg ccgagtgggt tggtgccccg ctggtgattc
     3001 cgatgcatta caatacgttc ccggcgattg cacaggatct gacggagttt gagcatgcga
     3061 ttgagtcaac gacgtcgatg ctggtggatg tggttgcacc gggcgacggg gttgaggtgt
     3121 cgccgcagca gtgagatgcc gcttttttgc ggctgttctt tttttgacgg gatgttcccg
     3181 gatttttcca aacagattta tgtgccaacg ttgaatgtga ggtaacagcg gtccgggaag
     3241 gttgtctgta tggtgtctct ggaaagtgtg tttcatctga aagccgaaaa aacaacggtg
     3301 ccccgtgagg ttatcgcagg tcttacgacg ttttttgcga tggcctacat tattattgtc
     3361 aaccctgcaa ttctgagcga aaccggcatg gagtggggtg ccgtgtttct ggcaacgatt
     3421 attgcggcaa ttgcaggtac actgatcatc ggtctgtatg caaatgtccc gtttgcccag
     3481 gcgccgggaa tgggactgaa cacattcttt g
//
LOCUS       DALTZZ010000004         1986 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_1154,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000004 DALTZZ010000000
VERSION     DALTZZ010000004.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="O0X12_00065"
     CDS             <1..417
                     /locus_tag="O0X12_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HJK52588.1"
                     /translation="LQPLAMSAYIAASMSRVRVVSAHPQFSDDGAIVGATEIVEIPVL
                     PIGYPGSEQQLLISRIGDGVESLDSLIYLMFPEIDKNSKRFRSERSRLSHHLSKLEAG
                     GFIVKTKYGRNISIHLTCLGQMFAQVISRGDVPQDN"
     gene            complement(427..1110)
                     /locus_tag="O0X12_00070"
     CDS             complement(427..1110)
                     /locus_tag="O0X12_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HJK52589.1"
                     /translation="MQKPLLEPSYTAVLFDMDNTLFDFIAAMKRGAAAAAETIGEGTG
                     DQLFSYYLRWKYHIEDHANLQDFMLDHHKFTVDGYLSAVKAFEDAKFSGLAPYPGIPD
                     LLASLKEKGYRLGIVTDAYEYAATERLRHTGLAEYFDTIVAYDSTGYKKPHHAPFEYA
                     MSLLGTLPHETVYVGDSIRRDIEPAKALGIAAVYAAYGDRNFFEQNRSCPPRVIVAHS
                     PEDIGKILL"
     gene            complement(1314..>1986)
                     /locus_tag="O0X12_00075"
     CDS             complement(1314..>1986)
                     /locus_tag="O0X12_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJK52590.1"
                     /translation="RTLGPDGFTQVKKVICPHADPDHCGAAVFLPAPPLTHPVTKQTR
                     DAGTRGFGSENRHEDLERAYTTTINTFSKMNIPKTVELCSTEPKGMHGLFPVIDDVSF
                     GGMEFEIWESLGGHIAGQLFLYEKNEGLLFTSDALINFASMTKARKDYCSIADYLVTS
                     VNVNSAVARTERQELMRIAKEIDADLKKKGKRLLLCCGHGAISMLDDAGNMVSACEPT
                     HYTAQ"
BASE COUNT          398 a          476 c          572 g          540 t
ORIGIN      
        1 cttcagcctc ttgcgatgag tgcctacatt gccgcaagca tgagcagggt tcgtgtcgtt
       61 tctgctcacc cccagttcag cgatgacggg gcgatcgttg gtgcaacgga gattgtggag
      121 attccggtac tgccgatcgg ctacccgggt tcggagcagc agcttttgat ctcccgtatc
      181 ggtgacggtg ttgagtcact cgattcgctg atctatctga tgttcccgga aattgacaag
      241 aactcgaaga ggttccgcag tgaaagaagc agactgtctc accatctctc caagcttgag
      301 gcaggcggat ttattgtgaa aaccaaatac ggcagaaaca tttccattca tctgacctgt
      361 ctcggccaga tgtttgccca ggtgatctcc cgcggtgatg tgccgcagga taactaaatc
      421 ttttttttat aagagaattt ttccgatatc ttccgggctg tgggctacga tgacccgcgg
      481 cgggcagctt ctgttctgct caaagaagtt ccgatcacca tacgctgcat agaccgcggc
      541 aatgccaaga gcttttgccg gttcgatgtc ccggcggatg gagtctccga catacactgt
      601 ttcatgcggc agtgtgccta acaggctcat ggcgtactca aagggtgcgt ggtgcggctt
      661 tttgtagccg gtcgagtcgt acgcaacgat ggtatcgaag tactctgcaa gacctgtgtg
      721 tctgagacgc tcggttgcgg cgtactcgta ggcgtccgtt acaatgccga gccggtaccc
      781 tttttccttt agggaggcga ggaggtcggg gatcccggga tacggggcaa ggccggaaaa
      841 ttttgcatcc tcaaatgctt ttacggcgga aagatagccg tccacggtga atttatgatg
      901 atccagcatg aagtcctgga ggtttgcatg gtcttcaatg tggtatttcc accgcagata
      961 gtacgaaaac aactggtctc ctgttccttc tccgatggtt tctgctgctg cggctgctcc
     1021 ccgtttcatt gcggcgataa agtcaaagag cgtgttgtcc atatcgaaca gaacagcagt
     1081 gtaggagggt tccagaaggg gtttttgcat aatgcgctga tagttgtttt tcgggaggct
     1141 acttagggat gccggttccg gcaccgaagg gctttccggg ttttttctgt caataaacca
     1201 cgggcagatt cattcactgt ttttgggaat atgattctgt ggtgtatctg ttcttttgtt
     1261 tgcgagaact ctgtaagaaa aaagtgattt tggtatgtgg tgtgagatcc gggttactgt
     1321 gcggtgtagt gggtcggttc acaggcggaa accatgtttc ctgcatcatc cagcattgag
     1381 attgcaccgt gcccgcagca gagcagaagc cgttttcctt ttttcttgag gtcggcgtcg
     1441 atctcttttg caatccgcat cagttcctga cgttcggtcc gggcgaccgc actgttgacg
     1501 tttacactgg tgacaagata atccgcaatg gaacagtagt ccttacgggc ttttgtcata
     1561 ctggcgaagt tgatcagtgc gtcgctggtg aagagcagtc cttcattttt ttcgtacagg
     1621 aacagctgtc ctgcaatgtg gccgccgagg ctttcccaga tctcaaactc catgccgccg
     1681 aacgacacat catcgatcac cgggaacagg ccgtgcattc cttttggttc ggtactgcac
     1741 agctccacgg ttttagggat gttcattttg gagaaggtgt tgatggtcgt ggtataggca
     1801 cgttccagat cttcgtggcg gttttcggat ccgaatcccc gtgttcccgc atcccgggtc
     1861 tgtttggtta ccgggtgggt aagggggggt gccggcagga atacggcagc gccgcagtgg
     1921 tcggggtccg catggggaca gatgactttt tttacctggg taaagccgtc gggccccaag
     1981 gtccgc
//
LOCUS       DALTZZ010000005         1842 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_1440,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000005 DALTZZ010000000
VERSION     DALTZZ010000005.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1842)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1842)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1842
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1440"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="O0X12_00080"
     CDS             <1..447
                     /locus_tag="O0X12_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HJK52591.1"
                     /translation="LDSFKVAGGIILFILGLQIVLGLEIGGKAAHEARAEQARDGAEL
                     LPGTVRKSMAGVIIGTPIMCGPGTITTVMIIGSEYGILITAVAIILALLVIWMILAFS
                     SRITAVLGETVIVFLSKIVGLLLTAIAVHTIWSGVVGLIGLTVAGL"
     gene            449..1120
                     /locus_tag="O0X12_00085"
     CDS             449..1120
                     /locus_tag="O0X12_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52592.1"
                     /translation="MVVFRPDLTEVIVFDLEAFVPPCDRKRKTGASLAVNPYREGHTL
                     LGGVIYRARPLEDVVIADFEHHWIWRDGSEEKVVKHLFSIFSDAWKHVATKKRVYCDP
                     LVLGIGISTFDLPFLAAKCQEYQVAPPEIIYETVCKLRVVDLATAGIGFLQIPRPVLH
                     PCTHNELANGFLGDRDQKPTGKRVWEMADEMEYAEIEKRCEGEVREMVAMMNVMTGLC
                     QKNRE"
     gene            complement(1123..>1842)
                     /gene="hisD"
                     /locus_tag="O0X12_00090"
     CDS             complement(1123..>1842)
                     /gene="hisD"
                     /locus_tag="O0X12_00090"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HJK52593.1"
                     /translation="AQAIAAMALGTETIKPVQKIVGPGNVFVTQAKMMLREYADIDFP
                     AGPSEIGIIADSSAEPAFIAADVLAQAEHDPNAACVLVTDSDKLASSVGEEIAAQIAT
                     APRREIMEKALAHSGYVIAEDMEDAAALMNRIAPEHLSIQTADPLATLSGIHHAGSIF
                     IGPYTPVACGDYASGTNHVLPTAGYAKSYSALDVNHFMKRSQVQIVTREGLETIGDVI
                     ETLATAEGLAAHANSVRVRRE"
BASE COUNT          444 a          426 c          525 g          447 t
ORIGIN      
        1 ctggacagtt tcaaggttgc cggaggaatc atcctcttca tattgggtct gcagattgta
       61 ctgggcctgg aaataggagg aaaggctgcg cacgaggcac gggcggaaca ggcacgtgac
      121 ggggcagaac ttcttcccgg aaccgtcaga aaatccatgg caggtgtcat tatagggaca
      181 ccaatcatgt gtggtccggg aacgatcaca acggttatga tcatcggctc agagtatggt
      241 attctcataa ccgcagtagc aatcattctt gctctgctgg tcatctggat gattctggca
      301 ttttcctcac ggatcaccgc agtccttgga gaaacggtga ttgtgtttct gtcaaagatt
      361 gtaggactgc tgctcacagc aattgccgtg catacaatat ggagcggggt cgtaggactt
      421 atcggactaa ctgtagcagg actctaagat ggtagtcttc cgacctgatc tgacagaagt
      481 catcgtgttt gatcttgagg catttgtacc tccatgtgat cgaaaacgaa aaacaggggc
      541 atcccttgcg gttaacccgt accgagaagg acatacgctg ctcggagggg taatctatcg
      601 agcgcgaccg ctggaggatg tggtcatcgc agattttgaa catcactgga tatggcggga
      661 tggctcggaa gagaaggtgg tgaaacatct gttttccatc ttttcggatg cctggaaaca
      721 tgtagccaca aaaaaacgtg tgtactgcga tccgctcgtt cttgggatag gaatatccac
      781 atttgatctg ccgttcctgg cagcgaaatg tcaggagtat caggtcgcac cgcctgagat
      841 catctatgaa accgtctgca aacttcgggt tgtggatctt gcaactgcgg gcattggatt
      901 tttacagatt ccacgaccgg ttctgcatcc ctgtacgcac aatgagctgg ccaacggatt
      961 tcttggagat cgtgatcaaa agccgaccgg aaagcgggtc tgggagatgg cagatgagat
     1021 ggagtatgca gaaattgaaa agaggtgtga gggagaagtg cgggagatgg ttgccatgat
     1081 gaatgtgatg accggcctgt gccaaaaaaa tagggagtag tattattctc tgcgaacacg
     1141 gaccgagttc gcatgagccg ccagtccttc agcggtcgca agggtctcga tcacatcgcc
     1201 tatggtctca agcccttcac gggtgacaat ctgtacctgt gatcgtttca taaagtgatt
     1261 gacgtcaaga gcggagtagc ttttcgcata cccggcagtt ggaagcacat ggttggttcc
     1321 tgatgcgtaa tcaccgcagg cgaccggtgt gtagggaccg ataaagatcg aacccgcatg
     1381 atgaataccg gataatgttg caagcgggtc ggcagtctgg atagagagat gttctggtgc
     1441 aatgcggttc atcagagcag ctgcatcctc catatcctct gcgatcacat atccagagtg
     1501 ggcgagtgct ttttccataa tctcccggcg cggagcagta gcgatctgtg ctgcgatctc
     1561 ttctccaacg gatgaggcaa gtttatcact gtcggtcacc agaacacagg ctgcgtttgg
     1621 atcatgctca gcctgagcca ggacatctgc cgcaatgaag gcaggttctg cagaggagtc
     1681 ggcaatgatt ccgatttcac tgggtcctgc cggaaagtca atgtctgcgt actcgcggag
     1741 catcatcttc gcctgggtaa caaagacgtt ccccggcccg acaattttct gcacgggttt
     1801 aatggtctca gttccgagag ccattgcggc aattgcctgg gc
//
LOCUS       DALTZZ010000006         2621 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_1450,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000006 DALTZZ010000000
VERSION     DALTZZ010000006.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..2070)
                     /locus_tag="O0X12_00095"
     CDS             complement(<1..2070)
                     /locus_tag="O0X12_00095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HJK52594.1"
                     /translation="MTSPKRIGKIEFGLLSPKAIRDMSVCKVIWPDTYDDDGFPYPKG
                     LMDPSLGVIDPGLRCKTCGQKQGDCPGHFGHIDLAKPVIHVGYTRLIRKLLRVTCRNC
                     GRLLLDKEEIERFSALEDDPYSAETIGEKDIKKERVCPYCGDQQFKINFEKPTSFVEV
                     VTETGEDGKIHKTEHKLTSADIRERLEKITDDDLRLLGIDPTVARPEWTILTVLPVPP
                     VTVRPSIILENGQRSEDDLTHKLVDIIRINQRFKENQDAGAPQLIIEDLWELLQYHVT
                     TYLDNEVAGCPPARHRSGRPLKTLSQRLKGKDGRFRGSLSGKRVNFSSRTVISPDPNL
                     SIGEVGVPLAIANEMSVPVRVTKQNIEDIRSMIRIGPNRPTLRDPCGANYVNRPDGRR
                     IRLIAGPEGNCETVAEMIEPGWKVDRQLRDGDIVLFNRQPSLHRMSIMSHHIKVMNGR
                     TFRLNPAVCPPYNADFDGDEMNLHVPQTEEARAEAELLVAVQENILSPRFGGPIIGGL
                     HDHISGIFLLTNQTKWYTKSQFLYLLKYTNMEHLPEPGKIEDGVPYWSNKQAFSMILP
                     NDVNMFYKATCCRNCDPCTKDTHCPHDAYVRIVDGQLLSGTIDKKSVGAMDGAILNRI
                     IRLHGVKRAREYIDDLTKLSIRAIMINGFSYGINDTDLGKEEYKQIRDVLDKAEADVA
                     QRINIYEQ"
     gene            complement(2072..>2621)
                     /locus_tag="O0X12_00100"
     CDS             complement(2072..>2621)
                     /locus_tag="O0X12_00100"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447126.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. The beta
                     subunit is part of the catalytic core which binds with a
                     sigma factor to produce the holoenzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HJK52595.1"
                     /translation="IGKGVADSTLTREQVLRHELAEAGFAHTGREVMYDGITGRRFQA
                     DIYIGVIYYQKLYHMVSTKMHARSRGPVQVLTRQPTEGRAREGGLRFGEMERDVMIGH
                     GAAMALKERLLDESDAVKQYVCARCGMVAMYDAKQKTTRCLACGAETDIYEVEMSYAF
                     KLLLDEMKSMGIAPRLKLEDMV"
BASE COUNT          589 a          708 c          672 g          652 t
ORIGIN      
        1 ctgttcatag atgttgatac gctgggccac atccgcctct gccttgtcga ggacatcgcg
       61 gatctgtttg tactcctctt taccgagatc ggtatcgttg ataccgtagg agaaaccgtt
      121 gatcatgatt gcacgaatcg acagcttggt cagatcatca atgtactcac gggcgcgttt
      181 cacaccatgg agacggatga tacggttcag aatcgcaccg tccattgcac cgacagactt
      241 cttgtcaatt gttccggaaa gcagctgacc atcaacaatt ctcacatatg cgtcatgcgg
      301 gcagtgcgta tcctttgtac agggatcaca gtttctgcag caggttgcct tgtagaacat
      361 gttgacatca ttcggcagaa tcattgagaa ggcctgtttg ttgctccagt agggcactcc
      421 atcctcaatc tttccgggtt caggcagatg ctccatgttc gtgtacttca gcagatacag
      481 gaactgggac ttggtgtacc atttggtctg gtttgtcagg aggaaaattc ctgaaatatg
      541 gtcgtgcaga ccgccaataa tcggaccgcc gaaacgcggg gagagaatgt tttcctgcac
      601 agccacgaga agctctgctt ccgcacgtgc ctcttcggtc tgcggaacgt gcaggttcat
      661 ttcatcacca tcaaaatctg cgttgtacgg aggacagaca gccgggttca ggcggaaggt
      721 tctgccgttc atcactttga tgtgatggga catgatcgac attctgtgta aggacggctg
      781 acggttgaac agtacaatat ctccgtcacg gagctggcgg tcaaccttcc atcccggctc
      841 gatcatctct gcaaccgttt cgcagttacc ttctgggcca gcaatcaggc ggatacgacg
      901 accatccgga cggttgacat agtttgctcc gcacggatca cgcagggtag gacggtttgg
      961 accgatgcgg atcatagacc ggatatcctc gatgttctgc ttggtaacac gaaccggaac
     1021 cgacatctca tttgcaatgg caagcggcac accaacctca ccaatcgaaa ggttcggatc
     1081 aggtgagata acagtacgtg aggagaagtt cacacgttta ccggaaaggg atccacggaa
     1141 tcggccgtcc ttacctttca ggcgctgaga cagcgtcttc agcggtcttc ccgaccggtg
     1201 acgggcaggc ggacatccgg caacttcgtt gtccagatag gtcgtgacgt ggtactgcag
     1261 aagttcccac agatcctcaa tgatcagctg cggtgcacct gcgtcctggt tttccttgaa
     1321 cctctggttg atacggataa tatccaccag cttatgggtc agatcatcct ctgatctctg
     1381 accgttctcc agaataattg acggacgaac ggtcaccggc ggcaccggaa gaacagtaag
     1441 aatggtccac tccggcctgg caacggtcgg gtcaattcca agcagacgga gatcatcgtc
     1501 ggtgatcttc tccagtctct cacggatatc tgcggaggtg agtttgtgct cggtcttgtg
     1561 aatcttgcca tcctcaccgg tctcggttac aacttcaaca aagctcgtcg gcttttcaaa
     1621 gttgatcttg aactgctgat caccacagta cgggcagaca cgctccttct tgatatcctt
     1681 ctcgccgatg gtctcagccg agtaaggatc gtcttcaagt gcactgaagc gttcaatctc
     1741 ctctttgtca agcaggagtc tgccgcagtt gcggcaggtc acacgcagaa gttttctgat
     1801 gagtctggtg tatcctacgt gaatcaccgg tttggcgaga tcaatgtgac cgaagtgacc
     1861 agggcagtct ccctgcttct gaccgcaggt tttgcagcgg agacccggat cgataacgcc
     1921 aaggctcggg tccatcagac ctttcgggta cgggaaacca tcatcatcat aggtgtctgg
     1981 ccagatgacc ttgcagacac tcatatcgcg gatcgctttt ggggagagca gaccgaactc
     2041 aatctttccg attctctttg ggctggtcat gttacaccat atcctccagt ttcagtctgg
     2101 gtgcaattcc catactcttc atttcatcaa gcagcagctt gaacgcataa ctcatctcga
     2161 cttcgtagat gtcagtctcg gcaccgcagg caagacagcg ggtggtcttc tgctttgcat
     2221 cgtacatcgc aaccataccg cagcgggcac agacatactg tttcactgca tcggactcat
     2281 caagcagacg ttccttcaga gccattgctg caccgtgtcc gatcatcaca tcacgttcca
     2341 tttctccgaa acgaagacct ccttcacggg cacgtccttc ggttggctga cgcgttaaga
     2401 cctggaccgg tccgcgtgaa cgtgcgtgca tcttcgtgga taccatgtgg tacagcttct
     2461 ggtagtaaat gacgccgatg taaatatcag cctggaatct gcggccggtg ataccatcat
     2521 acatgacttc acgaccggtg tgggcaaatc cggcttcagc cagttcatgg cggagaacct
     2581 gttcacgggt aagcgtgctg tccgcaacac cttttccaat c
//
LOCUS       DALTZZ010000007         2289 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_1470,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000007 DALTZZ010000000
VERSION     DALTZZ010000007.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1470"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="O0X12_00105"
     CDS             <1..134
                     /locus_tag="O0X12_00105"
                     /inference="COORDINATES: protein motif:HMM:NF039904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HJK52596.1"
                     /translation="TRQGQWRLGGYAPAELRERVSDAAREVLALRRATKQHKLTGII"
     gene            140..688
                     /locus_tag="O0X12_00110"
     CDS             140..688
                     /locus_tag="O0X12_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HJK52597.1"
                     /translation="MKRIVVGVTGASGTLYAKRLIEALTKTDGVEVYLIVSDTARTVA
                     ELEGVDLSGYPVQYEENCDLSAGIASGSFLFDAMIVIPCSMKSLAAIANGYSTTLIAR
                     AADVALKERRKLILVPRESPYSRVHLTNMLAAHDAGAVIMPASPPLYTHPQTITDLAD
                     MIAGRVLDHCGIPHTIGSRWKE"
     gene            685..1926
                     /locus_tag="O0X12_00115"
     CDS             685..1926
                     /locus_tag="O0X12_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HJK52598.1"
                     /translation="MRDFIKRMMAEGLVEEITEPVSSVYEAPKRAFTSKKMLYFHNCD
                     GHRCVMNAIYDRKSLSVALGIPEEKLVKTLAACTYAGKTHDAGKLSFGPAKLSDLPIM
                     KHFPKDAGRYLTSGIVFSAWGGVENASIHRMEVLDDTHLIGRIVEGRHTYKLLQQAKA
                     AGEKLPIAVVIGAHPAVTFAACTRVPEGKEMAYAAEILGEDMPLYECPNGIRVPDAEI
                     VLYGYMTAELHEEGPFVDISGTYDPIRMQPVIELCGMVCKPDYIYHGIVPAGAEHKML
                     MGAPYEPRIYQAAANVTNVRDVFLTPGGAGYFHAVVQVKKMTNGDGKNVIMAAFAAHT
                     SLKHVVVVDEDINIYDPNDVEFAIATRVRADQDVMIISGVRGSSLDPCRIGDGMNVKM
                     GIDATMQLGHEDEFIRADWNE"
     gene            1940..>2289
                     /locus_tag="O0X12_00120"
     CDS             1940..>2289
                     /locus_tag="O0X12_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HJK52599.1"
                     /translation="MELDKYDQALLDGEYGESRQKMMEILLALGKIYDAERFIPVKSV
                     QVSGASFKTIGEAGLEWLSSIDAKAVVPAFLNPIGMPRTGWEEIGIPQSFAEKQILVN
                     EAYIRLGIRPTCTCT"
BASE COUNT          602 a          564 c          647 g          476 t
ORIGIN      
        1 ccacgcgtca gggacagtgg cggctcgggg ggtatgcgcc agccgaactc cgcgagcgcg
       61 tgagtgatgc agcccgcgag gtacttgcac tgcggcgtgc aacaaaacaa cataaactca
      121 caggtataat ataagaagga tgaagcggat cgttgtcgga gtcaccggag cttccgggac
      181 gctgtacgca aaacggctga ttgaagccct cacaaaaacg gacggcgtcg aggtgtatct
      241 gatcgtctcc gacaccgcaa gaaccgtcgc agagctggaa ggagtggatc tttccggcta
      301 cccagtacag tacgaggaga actgtgatct ctccgcagga attgccagcg gatcattttt
      361 gtttgatgcc atgattgtta tcccgtgcag catgaagagc cttgcagcaa ttgcaaacgg
      421 ctactcaaca accctgatcg cacgggcggc ggatgttgcc ctgaaagagc ggcgaaaact
      481 gatccttgtt ccaagagaga gtccgtactc ccgggtacat ctgaccaata tgcttgccgc
      541 ccatgatgca ggagcggtaa tcatgcccgc ttcccctccg ctgtacacac acccgcaaac
      601 gatcactgat cttgccgata tgatcgcagg gcgcgtactg gatcactgcg gaatacccca
      661 cactattgga tcacggtgga aagaatgaga gattttatca aacgaatgat ggcagaagga
      721 ctcgttgaag agataaccga gcctgtttct tctgtctatg aagcaccgaa gcgggcattt
      781 accagtaaaa agatgctcta cttccacaac tgcgacggac acagatgtgt aatgaacgcg
      841 atctatgatc gtaaaagcct ctcggttgca ctgggaattc cggaggaaaa actcgtcaaa
      901 acgcttgctg catgtaccta cgcagggaaa actcatgatg caggaaaact ttccttcggt
      961 ccggcaaagc tctcggacct gccgatcatg aaacacttcc ccaaagatgc aggccgatac
     1021 ctgacctcag gaattgtgtt ctccgcatgg ggaggggtag agaacgcatc catccaccgg
     1081 atggaggttc ttgacgacac ccatctgatc ggaagaattg tggaaggccg tcacacgtac
     1141 aaacttttgc agcaggcaaa agctgccgga gaaaaacttc cgatcgcagt tgtgatcggc
     1201 gcacacccgg cggtaacctt tgccgcatgt accagggttc cagaaggaaa ggagatggca
     1261 tacgccgcag agatccttgg agaggatatg ccgctgtatg aatgtccgaa cggcatccgt
     1321 gttccggatg cagagatcgt cctctacgga tacatgacag cagagctgca tgaggaagga
     1381 ccattcgtgg acatcagcgg aacatatgat cccatccgga tgcagccggt aattgaactc
     1441 tgcgggatgg tctgtaaacc ggactatatt taccacggta ttgttcccgc aggagcagaa
     1501 cacaagatgc tgatgggtgc cccctatgag ccgcgcatct atcaggcagc ggcgaacgta
     1561 acaaacgtcc gtgacgtatt ccttaccccg ggaggagcag gatatttcca tgcggtggtt
     1621 caggtcaaga agatgacaaa cggtgacggt aaaaacgtca tcatggcagc attcgcagca
     1681 cacacctcgc tcaaacatgt agtggtcgtg gacgaggaca tcaacatcta tgatccaaat
     1741 gatgtggagt ttgcaattgc aacacgggtc cgggcggatc aggacgtgat gatcatctct
     1801 ggtgtccgcg gttcctcgct tgatccctgc cggattggcg acggcatgaa tgtgaagatg
     1861 ggaatcgatg caacaatgca gctcgggcat gaggatgagt ttatccgtgc agactggaat
     1921 gaataagtaa ggagtgaata tggaactgga taagtacgat caagcgctgc tggatggaga
     1981 gtacggcgaa agccgccaga agatgatgga aattctgctg gcactcggaa aaatttacga
     2041 tgccgagcgg tttattccgg taaagagtgt tcaggtttcg ggagcatcgt tcaagaccat
     2101 cggcgaggcg ggccttgagt ggctgtcaag cattgatgca aaagcagtgg tgccggcatt
     2161 tctcaacccc ataggcatgc ccagaaccgg ttgggaggag attggtattc cgcagagttt
     2221 tgcggagaag cagattctgg taaacgaggc atacatccgc cttggtatcc ggccgacctg
     2281 tacctgtac
//
LOCUS       DALTZZ010000008         5378 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_1623,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000008 DALTZZ010000000
VERSION     DALTZZ010000008.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5378)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5378)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5378
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="O0X12_00125"
     CDS             <1..465
                     /locus_tag="O0X12_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HJK52600.1"
                     /translation="ALYHVNEITPETRFPFFKQHIAEDEREVVTIEVADVLNVFKEIE
                     VSAVAIGCPHLSRDEVARVAELLSGKKTVMPFYVFVAEEMRQTCADELAVIAKSGAKV
                     VPDTCMVVSPLMDHTGPVMTNSGKAFSYLPGMCGVTPRMGTLEDCVRVGCGE"
     gene            466..1155
                     /locus_tag="O0X12_00130"
     CDS             466..1155
                     /locus_tag="O0X12_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HJK52601.1"
                     /translation="MSFLEKISPFERRDLLLAWFCLAVAFTLGISGGLALVTEVGKIS
                     VDMLVLTFVVAVITVGLSFVLHELAHKFMAIRYGYWAEFRKNTQMLLIAVVVAVVTGI
                     VFAAPGATLINTAGREMTKKENGVISLAGPLTNLILAVPFIVCLIAGVLLGGASISAF
                     TVPGFLFYLGMVGFQVNAMLAFFNMLPVGPLDGRKILRWNAVAFVALIAVSLLALYVS
                     LQPYMFVALLL"
     gene            1209..2102
                     /locus_tag="O0X12_00135"
     CDS             1209..2102
                     /locus_tag="O0X12_00135"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HJK52602.1"
                     /translation="MQLPKLLPTTVVGSFPCVKGTGLSTLFDPYKKAVQFAVAEQIRA
                     GVDIISDGQVRADMVQAFISKLPGISGNTVTSTVGISEKPITVADTRYALTKTKYVKG
                     ILTGPCTLAYALKIETPAYRNRDELVLDLATALHSEAKFLAATGAYMIQVDEPILSTG
                     VMDIETAKEALKIIFRDIETPSCIHTCGRLNTISSEWTRLPVDVIDFEYSVSPDNLSD
                     ISRHDLRNKKIGCGCVKSSEPEVESVAEIEKRIRSCVEVFGEENILIDPDCGLRMHTP
                     EVAFAKLKNMCDAAKNVRAEL"
     gene            complement(2167..2931)
                     /locus_tag="O0X12_00140"
     CDS             complement(2167..2931)
                     /locus_tag="O0X12_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HJK52603.1"
                     /translation="MEDIMQYIPGDRFVHRLSPMTKIIFAVGMMFAAIFTTDLAILAG
                     LIAFVLIIAAAGGLLRVLLRQVPLLIILGLALVVLTVLTNATGDVIIYLLPNGLLPVT
                     TGAILFAVQMALRFAVLIFAFQLLVISTQPRDLVNALYTLRVPGDYALMFLIAIRFIP
                     TLQREGARINEAQLSRGYFPGGGVFGKVKQLGPVMLPLMLNSLAKADTLGLTIDMRGY
                     RLASEYRRKLVYHAADAGMILLVILVLAGVAAVTFL"
     gene            complement(2933..4390)
                     /locus_tag="O0X12_00145"
     CDS             complement(2933..4390)
                     /locus_tag="O0X12_00145"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HJK52604.1"
                     /translation="MQKTRSVTRAEHEVPASTTAPVILDHVGFTYQAAASPSLDDVSL
                     TIREGEFVVINGPSGSGKTTFARAVSGVLVHAYGGDMTGSITIAGKYADEYEDVTDLS
                     KQVGMVFDDADAQLIFTTVEEEILTGLETRGLSVDEVAKKLDEIYKITCTGHLKDRAP
                     HALSGGQKQRVAMAAALSRETPILVLDEATSELDKKARRQVYMLLKSLTDRGHIVVLV
                     EHMTNETLGFATRMIRLENGKVVYDGEPVAETFICPEIPRTSSSGEIILAAEHLTHRF
                     GEVLALDDVSLTFAKGEIVAILGENGSGKTTLVKHLNGLLRPDSGTVLLNGTDITGKS
                     VSEIAKTVGLVFQNPDTMLFENTCEKEILFGLKNVGNPDPAAARAALAAVGLEGKAEV
                     NPRHLSRGERQRLALACVLAMDQQVVIMDEPTTGLDMVESFEIMKVLTGMRNTGKTIL
                     MVTHNPVLAEKFSDRVVEMETGRVTHIRVPANGGA"
     gene            complement(4376..4897)
                     /locus_tag="O0X12_00150"
                     /pseudo
     CDS             complement(4376..4897)
                     /locus_tag="O0X12_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4922..>5378)
                     /locus_tag="O0X12_00155"
     CDS             complement(4922..>5378)
                     /locus_tag="O0X12_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HJK52605.1"
                     /translation="ALRGPAVAKGYWNMPEETARAFLPDGWFLTGDVGYLDKDHRLCL
                     TDRKKDMIVMSGWKIYPTEVEEALLKYPDVAEIAVFGIPHPHRGEIPVAAVVWRAGRD
                     GSDKEAGLLAFAKEHLAGYKVPRKLYTVDALPRVNGWKLLRRELRETFA"
BASE COUNT         1316 a         1398 c         1451 g         1213 t
ORIGIN      
        1 gccctctacc atgtcaatga gattacaccg gaaacacggt tcccgttctt caagcagcac
       61 atcgccgagg atgaacgtga agtggtgaca attgaggtgg cagacgtact gaatgtgttc
      121 aaggagatcg aagtatcagc ggttgctatc ggatgcccgc acctttcccg tgacgaagtg
      181 gcacgcgtcg cagaacttct ttccggaaag aagacagtga tgccgtttta cgtattcgtt
      241 gcagaggaga tgcgacagac ctgtgccgat gagctcgcag tgattgcaaa gtccggggca
      301 aaggttgtcc ccgacacctg catggtggtt tctccgctga tggatcatac aggacctgtt
      361 atgaccaact ccggcaaagc attctcgtat cttccgggaa tgtgcggcgt tactccccgg
      421 atgggaacac ttgaagactg tgtccgtgtc gggtgtggtg agtaaatgag cttcctggaa
      481 aaaatatctc cgttcgaacg acgcgatctt ctgcttgcct ggttctgtct ggcagttgcg
      541 tttacgctcg gtatctcggg aggccttgca ctggtcaccg aggttggtaa gatctcagtc
      601 gacatgctgg tgttaacctt cgtggttgcg gtcattacag ttggactgtc gtttgttctg
      661 cacgaactcg cccacaagtt catggcaatc cggtatggtt actgggcaga gttccggaaa
      721 aacacgcaga tgcttctgat cgccgtagtg gttgcggtcg ttacgggcat tgtgtttgca
      781 gcacccggag caacgctgat caataccgca ggacgcgaga tgacaaagaa agaaaacggt
      841 gtcatctctc ttgcaggccc gctgacaaac ctcatccttg cggttccgtt cattgtatgt
      901 ctgattgcag gagttctgct tggcggcgca tcgatcagtg cgttcactgt tccgggattc
      961 ctgttctatc tgggcatggt cggattccag gtaaatgcaa tgcttgcatt cttcaacatg
     1021 ctgccggtcg gaccactgga cggaaggaaa atcctccgct ggaatgcggt ggcattcgta
     1081 gccctgattg cagtgtcgct tcttgccctc tacgtctcac tccagccgta catgttcgtc
     1141 gcccttctgc tctgagaagt taggaacaat acaaactttt tttatctttc ttcccgaatg
     1201 ctgtagatat gcagcttccg aaacttcttc cgaccacagt tgtcggctcc tttccatgcg
     1261 tgaaaggtac tggtctttcc acactgttcg atccgtacaa aaaagcggta cagtttgcgg
     1321 tcgccgaaca gatccgggca ggggttgaca tcatctccga cgggcaggtg cgggcggata
     1381 tggttcaggc attcatctca aaacttccgg gaatcagcgg gaatacagtc accagtacag
     1441 tcggcatctc ggagaaacct atcacggtag ccgacacccg gtatgctctg acaaagacaa
     1501 agtacgtgaa aggaattctc accggaccct gcacactggc atatgcgttg aagattgaga
     1561 ctcccgcata tcggaacagg gacgaactgg tactggatct tgcaacagcc cttcactcgg
     1621 aagcgaagtt tcttgcggca accggcgcat atatgattca ggtggatgag ccgatccttt
     1681 cgaccggagt catggacatt gaaactgcaa aggaagcact caagatcatc ttcagggaca
     1741 ttgaaacacc gtcctgtatt cacacctgcg gcagactgaa caccatctcc tcggagtgga
     1801 caagactgcc ggtggacgta atcgacttcg agtactcggt aagccctgac aatctctccg
     1861 acatctcacg gcatgatctc cggaacaaaa agatcggctg cggctgtgtg aaaagttccg
     1921 agccggaggt ggagtcggtt gcggagatcg aaaaacggat ccgatcctgt gtggaagtgt
     1981 tcggagagga aaacattcta atcgatcccg actgcggcct gcggatgcac acgccggaag
     2041 tagcatttgc aaaactgaag aacatgtgtg atgcagcaaa gaatgtcagg gctgagctgt
     2101 aatccgcaga aacatttcaa tcatcttttt ttgaaaaatt gcggcagaaa cttggatgaa
     2161 taaaaattag aggaaggtta ctgccgcaac tcctgcaagt acaagaatta caagcagaat
     2221 cattcccgca tctgcggcat gatacaccag ttttctccgg tactcggatg ccaggcggta
     2281 cccccgcata tcgatcgtaa gaccgagcgt gtcagctttg gcgagcgagt tcagcatcag
     2341 aggaagcatc acaggcccaa gttgtttcac tttcccgaac acacctcctc ccgggaagta
     2401 tccgcgagaa agctgtgcct cgttgatgcg ggcaccttcg cgctgaaggg ttgggatgaa
     2461 ccggatagca atcaggaaca tcagtgcata atccccagga acacggagtg tgtaaagcgc
     2521 attcaccagg tcacgcggtt gggtggagat aaccagcagc tggaatgcaa agatgagaac
     2581 cgcaaaccgc agagccatct gcaccgcaaa cagaattgca ccggttgtca ccggcagaag
     2641 accgttcggc agcagataga tgatcacatc acccgtggca tttgtcagaa ctgtcaggac
     2701 gacaagggca agaccaagga tgataagtaa cggcacctga cggagaagta cccgaagaag
     2761 tccgccggca gcagcaataa tcagcacaaa tgcgatcagg ccggcaagga ttgcaagatc
     2821 cgttgtgaag attgcggcaa acatcatgcc gaccgcaaag atgatcttgg tcatggggga
     2881 cagtcggtgt acaaaccgat cgccggggat gtactgcata atatcttcca tttcacgcac
     2941 ctccgtttgc gggcacacga atatgcgtca cgcggcccgt ctccatctca acaacccggt
     3001 cggagaactt ctccgcaagt accggattat gggtcaccat cagaatggtc ttgccagtgt
     3061 tacgcatgcc tgttaagacc ttcatgatct caaacgactc caccatgtca aggccggtgg
     3121 tcggttcatc catgatcaca acctgctgat ccattgcaag aacacaggca agggcaagcc
     3181 gctgacgttc cccgcggctt aagtggcggg gattcacctc tgcctttccc tccagtccga
     3241 cagcagcaag tgcagcgcgg gctgcagcag gatctgggtt tccaacattt ttcaaaccaa
     3301 agagaatctc cttttcacag gtgttctcaa acagcatcgt gtcaggattc tggaacacaa
     3361 gaccgacggt cttggcaatc tcggagacgg attttccggt gatgtcagtt ccgttgagga
     3421 gaactgtccc gctgtctgga cgaagcagtc cgttgaggtg tttgaccagt gtggttttgc
     3481 ctgatccgtt ctccccgaga atagcaacaa tctcaccttt tgcaaaggta agcgagacat
     3541 cgtcaagcgc aagaacctca ccgaaccggt gagtgagatg ttctgccgca aggatgatct
     3601 cgccagatga agatgtcctg ggaatctccg ggcagatgaa tgtctctgca accggttcac
     3661 cgtcgtacac aacttttccg ttctcaagcc ggatcatccg agtagcaaac ccgagtgttt
     3721 cgtttgtcat gtgctcaaca agaacaacga tgtgaccgcg gtcggtgagg cttttcagca
     3781 gcatgtacac ctgccggcgt gcttttttat ccagctcaga cgttgcctcg tcaagaacaa
     3841 ggatgggcgt ctcacgggaa agagctgcag ccattgcaac gcgctgtttc tgaccgccgg
     3901 agagtgcatg cggcgcacgg tctttgaggt ggccggtaca ggtgatctta tagatctcat
     3961 caagcttttt tgcaacctcg tccacagaca gtccgcgggt ttcaagaccg gtgagaatct
     4021 cttcttccac ggttgtgaag atcagctggg cgtcggcatc gtcaaagacc atccctacct
     4081 gtttggaaag atcggtaacg tcctcatact catcggcgta ctttccggca attgtgatcg
     4141 agccggtcat atccccgccg taggcatgaa ctaacactcc agaaacagca cgggcaaacg
     4201 tggtttttcc ggagccagac ggaccgttga tcaccacaaa ctctccttcc cggatggtaa
     4261 gggacacatc atcaagcgac ggggatgcag cagcctgata ggtgaacccg acgtgatcga
     4321 gaataaccgg tgcagtagtg ctggcaggga cttcgtgttc tgctctggta acactgcggg
     4381 ttttctgcat gacagtagtt gcaggcattg caatgatctg agcgatgatg gtgttcacaa
     4441 ccgccgcact tgcaacgatg ggtaccatgg cgaccgcaaa cgcaatgacg gtcccgactg
     4501 ttgagtcagg cgaggttgca agcattacac agcaggtgat accgatgaag gagaaaccac
     4561 tcaccggagt tgcaatggca gtagccactg ccggagcaag ccgtgtgtga tccttgacca
     4621 gtttatagac cgcaagacac acgaccgcac cgatcggttc ggagatgagg tttcccagag
     4681 ggaagatgga gtgcgaaaag accgcacaga taataccggc cacaagaccg ataccgatcg
     4741 cttcccggaa cttcgggaca acgaggatga tcgcaaggca gtagaatgca atcgtaaggt
     4801 ttgcaacgat tgctcccgga acaaacagtg agatgtaccg ggcgatcgca ccggcagcaa
     4861 gcagaatacc taccagtgca atatcgcgtg atttcatgat tgatgacttc cgtattgata
     4921 ttcaggcgaa tgtttcacga agctcacgcc tgaggagctt ccatccgttt acccggggga
     4981 gggcatccac cgtgtaaagt tttcggggaa ctttgtatcc tgccagatgt tccttggcaa
     5041 atgcaaggag tccggcttct ttgtccgagc cgtcccggcc cgcccgccat acaacagcgg
     5101 caacgggaat ctcgccccgg tggggatggg gtatcccaaa gactgcaatt tctgcgacat
     5161 ccgggtattt gagaagggct tcctcaacct cggtcggata aattttccag cctgacatga
     5221 cgatcatgtc ttttttgcga tcggtaaggc agaggcggtg gtctttatcc agatatccca
     5281 catcaccggt caggaaccaa ccgtcaggga ggaatgcacg ggcggtttcc tccggcatgt
     5341 tccagtatcc tttggcaaca gcgggaccac ggagagca
//
LOCUS       DALTZZ010000009         2411 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_1655,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000009 DALTZZ010000000
VERSION     DALTZZ010000009.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="O0X12_00160"
     CDS             complement(<1..557)
                     /locus_tag="O0X12_00160"
                     /inference="COORDINATES: protein motif:HMM:NF021414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HJK52606.1"
                     /translation="MGFWNRFGAEDRTVCGVDWNALARISRKGDLLAQLKSYGMLFTP
                     DDLEEMLVRYGKKLVHVKKEYATALLASARVQIVDGYHRMMIAELASVHAEVRLPKSW
                     HGFVQSAAVQSRGKRLAELKYLIAAYTMYIAEEPAHPVGTPFPGGYTVELHEGVYYCP
                     VRAAQSEVDETLCRFCPAVQSRERDL"
     gene            complement(558..>2411)
                     /locus_tag="O0X12_00165"
     CDS             complement(558..>2411)
                     /locus_tag="O0X12_00165"
                     /inference="COORDINATES: protein motif:HMM:NF028231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HJK52607.1"
                     /translation="SADTVAELEDIYLPYRPKRKTRASVARDRGLAPLAAVLRMQDIR
                     LSPEAAALTFLTPDVPTTADALAGARDIIAEEISEDADLRGVIRGIYARSAHLSSAVI
                     KGKAEDSSVYAGYFAWDELINRAASHRVLAVMRGEREGVLRLHLAPPEETVLKELLPR
                     VVTGNGPASSEVATAAADAYRRLIAPSLEREFFNTIKERADTEAISVFAENLRHLLLA
                     APAGEKRVLAIDPGYRTGCKFVCLDATGGVVESGVFYPEAKRAQARETLIRLCKMHAI
                     DIIAIGNGTAGRETERFVKSISFGRPLPVLLTSECGASVYSASAEARREFLDLDITLR
                     GAVSIGRRVQDPLAELVKIDPKSLGVGQYQHDVDQNRLAAKLADVVERTVNAVGVNLN
                     TASPALLSYVSGIGPVLAEKIVAYRTEHGPFKNRRELLDVPGVGPKIFEQAAGFLRIR
                     NGDNPLDLTGVHPERYPVVEQMAADLGVLPGRLVGGTELLKRIEPERYVCPDCGLVTI
                     TDILEELANPGRDIRVTGEEEFSFDPAVHAITDLAEGMVLPGIVTNVTAFGAFVDVGV
                     HQDGLVHISELADRFVAHPSEIVTVGDRTRVVVLAVDISRSRISLSIKRAV"
BASE COUNT          619 a          692 c          589 g          511 t
ORIGIN      
        1 agatctcgtt cacggctctg tactgccgga cagaaccggc agagagtttc atcaacctca
       61 ctctgggctg cacggacagg gcagtagtat acaccctcat gcaactcaac agtataacct
      121 cccggaaacg gtgttccaac cggatgggcc ggttcctctg cgatgtacat ggtgtatgct
      181 gcaatcagat acttgagttc cgcaagccgt ttcccgcggg actgtacagc tgctgattgc
      241 acaaacccgt gccatgattt cggtagacga acttccgcat gaacggacgc aagctcagcg
      301 atcatcatac gatggtaacc gtccacaatc tgaacacggg ctgaagcaag gagagcagtt
      361 gcatactctt tttttacgtg tacgagtttt ttaccgtacc ggacaagcat ctcttcaaga
      421 tcatcgggag taaacaacat tccgtacgac tttaattgcg caagaagatc tcctttgcgc
      481 gagatacgtg caagggcatt ccagtcaaca ccacagacag tacgatcttc tgctccaaac
      541 cggttccaga accccattta caccgcccgc tttatactca gactaatgcg gctgcgggag
      601 atatcaaccg ccagaaccac tacacgtgtc cgatctccca ctgtcacaat ctccgaagga
      661 tgggcaacaa agcgatcagc aagttcgcta atgtgtacaa gcccgtcctg atgtaccccc
      721 acatccacaa atgcgccaaa tgcagtaaca tttgtaacga ttcccgggag taccattccc
      781 tcagcaagat cagttatcgc atgaacggcc ggatcaaagg agaactcttc ctctccagtt
      841 acacgaatat ctcggccagg gtttgccaac tcctcaagaa tatcagtgat ggtcacaagg
      901 ccgcagtccg gacagacata tctctccggt tcgatccgtt ttaggagttc tgtacctcca
      961 acaagcctgc ctggcaaaac accaagatcg gctgccatct gttccactac tgggtaccgt
     1021 tcaggatgca cgccggtcag atcaagcggg ttgtcaccat tcctgatacg gaggaatccc
     1081 gcggcctgtt caaaaatctt gggaccgaca cccggcacat ccaacagttc cctgcggttt
     1141 ttgaacggac catgttcagt acgataggca acaatttttt cggcgagcac aggaccaatg
     1201 cccgagacgt aagagagcag tgcagggctt gctgtattga gattgactcc tacagcgttg
     1261 accgtccgct caacaacatc agcgagtttt gcggcgagcc gattctgatc cacatcatgc
     1321 tggtactgac cgacaccaag tgacttcgga tcaatcttaa cgagctctgc cagtggatcc
     1381 tgcacacggc ggccaatgga cacagcacca cggagagtta tgtcaagatc cagaaactcg
     1441 cggcgggctt ctgccgacgc cgagtacaca gatgcaccgc actcacttgt cagcagcact
     1501 ggcagtggtc ttccaaagga gatactctta acaaatctct ccgtttcacg gccagcagtt
     1561 ccgtttccga tcgcaataat atcgatcgca tgcattttac agagccggat cagggtctca
     1621 cgtgcctgag cacgtttcgc ctccggatag aaaacaccgc tctctaccac accacctgtt
     1681 gcatcaaggc agacaaattt acaaccggtg cggtatcccg gatcaatagc aagcacccgt
     1741 ttctcaccgg ctggagctgc aagcagaaga tgacggagat tttcagcgaa caccgaaatt
     1801 gcctcggtgt cagcacgttc cttgatggta ttgaagaact cgcgctccag tgatggagcg
     1861 atcagacggc ggtaggcatc agccgctgcg gttgcaacct cagacgaggc aggaccattg
     1921 ccggtcacaa cacgcggaag cagctctttg agaacagttt cttccggggg agcaagatgc
     1981 agacggagaa caccttcacg ttctccccgc atcactgcaa gaacacggtg ggacgcagca
     2041 cgattaatca gttcatccca cgcaaagtac ccggcataca ctgaggagtc ctcagctttt
     2101 cccttaatca cagcggaaga aagatgggcg gaacgggcat agattccgcg aatcaccccg
     2161 cgaagatctg catcttcgga gatctcctcc gcaataatat cccgtgctcc ggcaagtgca
     2221 tcagcagtag ttggaacatc aggagtgaga aacgtcaaag cagcagcctc cggagaaagc
     2281 cgtatgtcct gcatgcgcaa aactgctgca agtggggcaa gcccgcggtc acgggcaacg
     2341 ctcgcccgag ttttgcgttt tggccggtag ggcagataaa tgtcctcgag ctctgcaaca
     2401 gtatctgcgg a
//
LOCUS       DALTZZ010000010         2578 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_1736,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000010 DALTZZ010000000
VERSION     DALTZZ010000010.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1736"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            23..1027
                     /locus_tag="O0X12_00170"
     CDS             23..1027
                     /locus_tag="O0X12_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HJK52608.1"
                     /translation="MKKDIYYRVDKRVQNGVVKYYLMREVNSDTRKFRASKLIKAGTP
                     PTDPEVARAILLYGFELEMKCCEKVVSYRVKNFKYQYEENEKIYSQLEKYRYLDLRYQ
                     NYLSEEEAARRISEREYHYIHESVKNVGCTFTYPEVVQMLETGKIPNGKYLRDINEIQ
                     NLYRCAVLRNKNPRKVTLALVLQIRNMILDNIEEPERISPEKKAAIQKHLNTYYQKIN
                     DGYHPAEQIIILYNNFRKLKPFKNGSKRTLREIIQYLILPIGYPLEYQSSQLIVQASE
                     MLTQHQTKISIPNIIEQYAEAGKISHLEEQIRKKIIERWDLQKNKNQKQLDLYLSDGC
                     "
     gene            1086..1436
                     /locus_tag="O0X12_00175"
     CDS             1086..1436
                     /locus_tag="O0X12_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HJK52609.1"
                     /translation="MINKLSFMGLVGLLGLLSIPTGDVRYCAFFAFFVFFGYLFTIED
                     ELFTAYVRKAASTAFFLQTALFFIIYAGTVLIGDAAVFKAAFPLTFAAGMFVFICLLV
                     IHEYREQRGVESGT"
     gene            1426..1629
                     /locus_tag="O0X12_00180"
     CDS             1426..1629
                     /locus_tag="O0X12_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HJK52610.1"
                     /translation="MALKTKIREYRVKQNITQEDLAKAVGVRRETIGHLENGKYNPSL
                     KLGFDIAKVLNVPVEELFEFTDD"
     gene            complement(1675..>2578)
                     /locus_tag="O0X12_00185"
     CDS             complement(1675..>2578)
                     /locus_tag="O0X12_00185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK52611.1"
                     /translation="YISLHWIFFINIPGGIVGVLAALFLIPKPDPADLPTRSKPFDSL
                     GAVYLAATLVTLLAGLEFGVSDGWTLPVLLLIVLSPVFGYLFLHRELRHPDPILSARL
                     LYNRTVMWAAVSTLCITLVYLGVIYVMPFLLTEEFLLPTALAGGIMLLPPVAMALVGI
                     PAGSLVRRCGCMFLCNSASVLLAVGMIFLGIAVFFSNMLLIFAGLVLVGLGMGLNEGP
                     SIQRITVHSPLELQGSSGGLIFTVMNVGCVLGVALFSVAAATGSGSADVYTPTGIAIA
                     CAVGFVVSVIALVTSKLARDTVMC"
BASE COUNT          827 a          631 c          583 g          537 t
ORIGIN      
        1 ccgccaatat actacacaaa ggatgaagaa ggacatctac tatcgtgtgg acaaacgagt
       61 gcagaacggt gtggtaaaat attatctgat gcgtgaggtc aacagtgaca cgcgcaaatt
      121 ccgcgcatcc aaactcatca aagccggaac tcctcctacc gacccggaag ttgcgcgggc
      181 aattcttctc tacgggtttg agcttgagat gaaatgctgt gaaaaagtcg tttcataccg
      241 agtaaagaat ttcaaatacc agtacgaaga aaatgaaaaa atctactccc agctggaaaa
      301 atacagatat cttgacctgc gctatcagaa ctacctttcc gaggaggaag cggcaagacg
      361 gataagtgag cgtgaatacc actacattca tgaatcggta aaaaatgttg gatgcacgtt
      421 tacgtatcct gaagttgtcc agatgcttga gacaggaaaa ataccgaatg gaaaatacct
      481 gcgcgatata aatgagattc aaaatctgta ccgctgtgct gttcttcgca acaaaaaccc
      541 ccggaaagtc acacttgccc ttgtccttca gatcagaaac atgatcctcg acaacattga
      601 ggaaccggag cggatcagcc ctgaaaaaaa ggctgccatt caaaaacatc tcaacaccta
      661 ttatcagaaa atcaatgacg gataccatcc cgcagaacag attatcatcc tgtacaataa
      721 cttccggaaa ctcaaaccat tcaaaaacgg atccaaacga acgcttcggg agatcataca
      781 gtacctgatt ctgccaatcg gctacccgct ggagtatcag tcgagccagc tgatcgtgca
      841 ggcgtctgag atgttaacgc aacaccagac aaagatcagc atccccaaca ttattgaaca
      901 gtatgccgag gcaggaaaaa tctcacatct ggaagaacag atacggaaaa aaataattga
      961 acggtgggat ttgcagaaaa acaaaaacca gaaacagctc gacctctatc tatccgacgg
     1021 ctgctgatgc ccattcgatg atgtgatgta tttatatcat tatgttacaa atatatcaca
     1081 caccaatgat aaacaaactc agtttcatgg gactggttgg attgctcgga ctcctttcga
     1141 taccaaccgg tgatgtacgg tactgtgcct tctttgcatt cttcgtgttt ttcggctacc
     1201 tattcaccat tgaggatgaa ctattcacag catatgtaag aaaagcagca tcaacagcgt
     1261 ttttcctcca gaccgcctta ttcttcatca tatatgccgg aacagtactt attggagatg
     1321 cagcagtgtt caaagccgca tttccgctga catttgctgc aggcatgttt gtattcatct
     1381 gtcttctggt aatccacgaa tacagagagc agcggggcgt ggaaagtggc acttaaaaca
     1441 aagattcgtg agtatcgtgt aaaacaaaat atcacccagg aagatcttgc caaagccgta
     1501 ggtgtcaggc gtgagaccat cggtcatctg gaaaacggaa agtacaaccc atctctgaaa
     1561 cttgggtttg atattgcaaa agttctgaac gtacccgttg aggaactctt tgagttcacc
     1621 gacgactaac actctttttt gataaaaaca tcgtcccata aaaggacaga tagatcagca
     1681 catcaccgta tcacgcgcaa gttttgaggt aacaagtgcg atcaccgaaa cgacaaaccc
     1741 aactgcacag gcgatcgcaa tgccggtcgg agtgtacaca tcagcactgc ctgaaccggt
     1801 agcggcagca accgagaaca gggcaactcc cagtacacac cccacattca tcaccgtaaa
     1861 gatcagaccc ccagaagaac cctggagttc aagcgggctg tgaacggtaa ttcgctgaat
     1921 gctgggtcct tcattcagac ccattccaag tccgaccaga accagaccgg caaaaatcag
     1981 aagcatgttt gaaaaaaata cagcaatacc caaaaagatc attccaacag caagaagaac
     2041 ggacgcgctg ttgcagagga acatgcagcc gcaccggcgc actaatgacc cggcaggaat
     2101 tcctaccagt gccattgcaa caggaggcag aagcataatg ccgcctgcta gtgcagtcgg
     2161 caaaagaaac tcttccgtca gaaggaacgg catcacgtag attacaccaa ggtagacaag
     2221 ggtgatgcac agcgttgaaa ccgcagccca catgaccgta cggttgtaaa gcagacgggc
     2281 cgaaagaata ggatcgggat gccggagctc gcggtgcaga aacaggtaac cgaaaaccgg
     2341 cgacagcaca ataagcagga gaacaggcag tgtccagcca tcggataccc cgaactccag
     2401 ccccgcaagg agcgtaacca gcgtagctgc aagatatacc gctccaagcg aatcaaaggg
     2461 tttggatctc gtaggtagat cggcgggatc gggttttggg atcaggaaca gcgcagctaa
     2521 aacacccact atacccccgg ggatgttaat gaaaaatatc cagtgcaagc tgatgtac
//
LOCUS       DALTZZ010000011         2698 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_1813,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000011 DALTZZ010000000
VERSION     DALTZZ010000011.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /gene="grpE"
                     /locus_tag="O0X12_00190"
     CDS             complement(<1..344)
                     /gene="grpE"
                     /locus_tag="O0X12_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HJK52612.1"
                     /translation="MEKKTETPEEKKPAAEETPVTTVEETPVDPVAELTKKYDELNDR
                     YLRLVAEFENYKKRTQRDAENTVRYANEKFARDMLDVLDNFERALKGGDDDLRSGLEQ
                     IHKLYLSVLSRNG"
     gene            complement(534..>2698)
                     /locus_tag="O0X12_00195"
     CDS             complement(534..>2698)
                     /locus_tag="O0X12_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HJK52613.1"
                     /translation="FGRVLRAAAKSIDAEKQRLNLLDFDDLIYRAVRLITENPAVLSE
                     LWERYRYILVDEVQDNDPRLTDLILRLAGSPAASDRLFIVGDLKQSIYRFRGADIRKN
                     SALFDAFPATSRVSLDVSFRTVPEIVHLVNTVFSQVFTGAARSYDVSYDALSANRTQD
                     TGSVQLLCRKKEQGEDTSTARIRESEDIAAWIAGSVRNQALLIKTKDGVSRPAEYGDI
                     AVLLNTRTPLPQLTAALQKFSVPYLIYKSGMLYQQQETYDFSNLLEVIINPENDAALY
                     GLLRSPWFGISDADLCRLRTGRFSLWKDVRDSPLAESVVQTIRHWQKLSLQQPIGRFL
                     RTVLRESGILTTYAAMQGGDLIIVNLEKLISLLEGTDSLIHAVFQLRENILNAEIKED
                     EGDVIAGNRVQILTIHAAKGLEYPVVIAAFTGDGKSDKLDDWIFDEQLGPAFSLQVRK
                     SDAEDVSVKTFVQNYLKPDEITAEKMEIKRKLYVALTRARDHLIISGTYMNQDGSASK
                     NSFLSLLDKDVLFSCGATECRIPAIPETESRNTAEDVLENWVDTPTVFCEDTKSDVPY
                     IRPSMQTSSAPGSEEAMIRGSALHAVFAGLSPNRACERYGISLEDAASFAAMREEFFS
                     SPLYQGCKEDYAELSLQFPLGDTWVRGRVDRILVHEERIVVVDFKSGHRESCGSLMEG
                     YKQELLTYLAGVEQLFGKRPEGYLYFPEDEEKILPVSF"
BASE COUNT          665 a          664 c          691 g          678 t
ORIGIN      
        1 ccgttgcggg aaagaacaga cagatacagt ttgtgtatct gttcaagtcc tgacctgaga
       61 tcgtcgtcac cgcctttgag tgcacgttcg aagttgtcca aaacgtcaag catgtcgcgg
      121 gcaaactttt cgttcgcata acgaacggtg ttttctgcat cacgctgggt gcgttttttg
      181 taattttcaa actctgcgac gagacggaga taccggtcgt tgagttcatc atattttttg
      241 gtgagctcgg caaccggatc aaccggagtc tcttcaacgg tagtcaccgg agtttcctct
      301 gccgccggtt ttttttcttc cggggtttct gtttttttct ccatggtaat tacctaatac
      361 ttgtacaact aattatttgt attgcagttc tatataaata tttgtattaa agggatttac
      421 ggatatttgt tgttttacaa aaacaggaca tttacaatcg attttctaaa aattcagatc
      481 acattgacag ttaccaaagg taagggagcg cagagaagat ccgttcccct cttttagaaa
      541 ctgacaggta agatcttctc ctcgtcctca gggaagtaga ggtaaccctc cggccttttc
      601 ccaaacagct gctccactcc tgcaagatac gtgagaagct cttgtttgta accttccatg
      661 aggctgccgc aactttcccg atgaccggac ttgaaatcaa caacaacgat cctctcctca
      721 tggacaagaa tgcggtcaac cctgccccgc acccaagtat cgcccagcgg gaactggagg
      781 gaaagctcgg cataatcctc tttgcatccc tgatagagcg gggaggagaa aaactcctcc
      841 cgcattgcag caaagcttgc tgcatcctct aaagatatcc cgtacctttc acaggcacga
      901 tttggtgaca gaccggcaaa aaccgcatga agagcagatc cccgtatcat agcctcctca
      961 ctgccgggag ctgaggaggt ctgcatggaa ggacggatgt acgggacatc gcttttcgta
     1021 tcctcacaaa aaacggttgg cgtatcgacc cagttctcta acacatcctc cgccgtgttc
     1081 cgggactctg tctcagggat cgcaggtatc cggcattcag ttgccccgca ggagaagaga
     1141 acatctttgt caagaagaga taagaaggag tttttacttg ctgaaccgtc ctggttcatg
     1201 taggttcccg agatgatgag atgatctctt gctcgggtga gagcaacgta gagttttcgt
     1261 ttgatctcca tcttttctgc ggtgatctca tccggcttca ggtagttttg gacaaatgtt
     1321 ttgacagaga catcttcggc gtcagacttt cgcacctgaa gggaaaacgc aggaccaagc
     1381 tgttcatcaa agatccagtc gtcaagtttg tcacttttcc cgtccccggt aaaagctgca
     1441 atgacaaccg ggtactccag acctttggct gcgtgaatcg taagaatctg aacacggttt
     1501 cccgcaatga catcgccttc atcctctttg atctcagcat tcagaatgtt ctcccgcagc
     1561 tggaagacag cgtgtatcaa agagtcggta ccttccagaa gagagatgag tttctccaga
     1621 ttgacaataa tgagatcccc tccctgcatg gcggcatagg tggtaaggat gccggactct
     1681 ctgagaacag ttcgcaaaaa ccttccaatg ggctgttgaa gtgagagttt ttgccagtgc
     1741 cggatggtct gcacaacgga ctcagccaga ggactgtcac ggacatcctt ccataaggag
     1801 aaacgaccgg tccgcagacg gcagagatcg gcatcgctga tcccaaacca gggtgaccgg
     1861 agaagaccat acaatgctgc atcattttcc ggattgatga taacctcaag aaggttggaa
     1921 aagtcatatg tctcctgctg ctgatagagc atgccgcttt tgtagatcag atacggtacc
     1981 gagaactttt gcagggctgc ggtgagctgg gggagcggtg ttcgggtgtt aaggagaacg
     2041 gcaatgtcac cgtactctgc gggacggctt acaccgtctt ttgtcttgat gaggagggcc
     2101 tggttacgga cggatccggc aatccaggct gcaatgtctt cgctttcccg tatacgggcg
     2161 gtgcttgtgt cctccccctg ttcttttttc ctgcaaagga gctggacaga acccgtgtcc
     2221 tgggtacggt ttgcagatag agcatcatac gagacatcat aggatcgtgc agcaccggtg
     2281 aagacctggg agaataccgt gttaacgaga tgaacgatct ccggaaccgt ccggaaactt
     2341 acatcaagac tgacacggga tgtcgcgggg aatgcatcaa agagtgcgga atttttccgg
     2401 atgtcggctc cgcggaaccg gtagatggac tgcttgagat cgccgacgat gaaaaggcgg
     2461 tcggatgcag ccggagaacc ggcaagacgg aggatcagat ccgtgagacg gggatcattg
     2521 tcctgtactt catcaacaag gatgtatcgg tatcgttccc aaagctcgct taggactgca
     2581 ggattctcgg tgatgagccg gactgcccgg tagataagat cgtcaaaatc aagaaggttc
     2641 aaccgctgtt tttctgcatc aatgctcttc gcagcagccc tgagcacccg tccaaagg
//
LOCUS       DALTZZ010000012         1698 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_1872,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000012 DALTZZ010000000
VERSION     DALTZZ010000012.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1872"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="O0X12_00200"
     CDS             complement(<1..658)
                     /locus_tag="O0X12_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HJK52614.1"
                     /translation="MWKKISAVLIPTLIAAALIVVMLARVWDDLLIAVQHLVPAYLIG
                     AILICLAAWYLRGWRYQYILKKLGTQIGLNFSTACIYVSQTANLIIPARLGDFVRLFI
                     LKHEKETPYTNSFTSVVAERVYDVIIIAVLGLFALPMLISLVPAWFVWMIVFVLGAGA
                     VFFLFLLLSRRLHAENKIFKKILEVLDQLRQVSSTPASLAALSLSSAIIWMMDVTICY
                     L"
     gene            complement(642..1358)
                     /locus_tag="O0X12_00205"
     CDS             complement(642..1358)
                     /locus_tag="O0X12_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HJK52615.1"
                     /translation="MNPVHITVVLPVYNDVEALRSAIPKSLEALTEFGKTFELIVAED
                     GSSDGSRELVEEWERKDPRVRLLHSDERQGRGRALNRALAESNGEIFCYYDVDLATDI
                     KHLPELLTRIEDGAAVATGSRLMPESKIIRSGDREFASRSYNTLVRLFLGSRLRDHQC
                     GFKAYRADVLRELVPKIRAPHWFWDTESVVLAQRAGLRVDEFPVVWTQGPGTTVRFKD
                     VYGMGMDILKMWWRLHVEKN"
     gene            complement(1355..>1698)
                     /locus_tag="O0X12_00210"
     CDS             complement(1355..>1698)
                     /locus_tag="O0X12_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amine oxidase"
                     /protein_id="HJK52616.1"
                     /translation="NFVPYDWYGEHVVYLASYFKDDPATDLKEKMIDDFCNRFGIGKE
                     IIHHADLYIDKFAGPVYVTGYRNMIPNTDAGHNLFIAGMYSVENYPERSMEGSVRAGH
                     RVAALLKEKLS"
BASE COUNT          451 a          442 c          392 g          413 t
ORIGIN      
        1 caagataaca gatggtcaca tccatcatcc agatgatagc agatgaaagt gataacgctg
       61 caagtgatgc tggcgttgaa gacacctgtc tgagctgatc taacacctcc aaaatttttt
      121 tgaagatttt attttccgca tggagtctgc gtgagagaag aagaaaaagg aaaaatactg
      181 cacctgctcc cagaacgaaa acaatcatcc agacaaacca tgcgggaacg aggctaatca
      241 gcattggcaa tgcaaatagt ccaagaactg cgatgatgat aacatcatac acacgctctg
      301 cgacaactga ggtgaaactg tttgtataag gcgtttcttt ctcgtgtttt agaataaaga
      361 gccgaacgaa gtcacccagc cgtgcgggaa tgatcagatt cgctgtttgg gatacataaa
      421 tgcaggcggt ggaaaagtta agacctattt gcgtccctaa tttctttagg atatactggt
      481 accgccagcc acgcaggtac catgcggcaa gacagatcag aatcgctccg ataaggtagg
      541 cgggaaccaa atgttggact gcgatcagca gatcatccca gactcttgca agcataacca
      601 caatcagtgc agcagcaata agagtgggga taagtacagc actaattttt ttccacatgt
      661 agccgccacc acatttttag aatatccatt cccatcccgt acacgtcctt gaaccggact
      721 gttgttcccg gtccctgtgt ccaaacaacc ggaaattcgt ccacgcggag tcctgcacgc
      781 tgtgcaagta ccactgactc ggtatcccag aaccagtgag gcgcacggat ctttggaacc
      841 agttcccgca gcacatctgc ccggtatgcc ttgaatccgc actgatgatc ccggagacgg
      901 cttccaagaa acagccggac cagtgtattg taactgcggc tcgcaaactc ccggtcaccg
      961 ctccggataa ttttactctc cggcatcaga cgcgatccgg tagcaactgc tgcaccatct
     1021 tcgattcggg tcaggagttc aggaagatgt ttgatgtctg ttgcaaggtc cacgtcatag
     1081 taacagaata tttcgccatt tgactccgca agtgcacggt tcagtgctcg tccccgtccc
     1141 tgccgttcgt cgctgtgcag cagacggaca cgcggatctt tccgctccca ttcctcaact
     1201 aactcgcggc tcccgtcgga actcccatcc tcggcgacaa tcagttcaaa agtttttccg
     1261 aactcagtca acgcttcaag cgattttggg attgcagatc gtaatgcctc aacatcatta
     1321 tagaccggca gcactacggt aatgtgtacg ggattcacga aagtttctcc ttgagcaggg
     1381 cagctacgcg gtgtcctgca cgaacagaac cttccatgct ccgttcggga tagttctcca
     1441 ctgagtacat tccagcaatg aacagattgt gtccggcatc ggtattgggt atcatgttcc
     1501 gataaccggt tacatatacg ggccccgcga acttgtcaat gtacaggtcg gcgtgatgaa
     1561 ttatctcttt tcctattcca aacctgttac agaaatcatc aatcattttc tctttgagat
     1621 cggttgccgg gtcgtctttg aagtacgagg caagatacac tacatgttcg ccgtaccagt
     1681 cataagggac aaagttcg
//
LOCUS       DALTZZ010000013         2015 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_2510,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000013 DALTZZ010000000
VERSION     DALTZZ010000013.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2510"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="O0X12_00215"
     CDS             complement(<1..1065)
                     /locus_tag="O0X12_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJK52617.1"
                     /translation="MDDTDTTAGHAGGADLGTAPVISPGVQEPETPARAEPAVPKTYA
                     SDSFDPEFFGGIQFNTTADIAIPPSLIDQVIGQEHAVEVIKKAATQRRHVMMIGSPGT
                     GKSMLAKAMAELLPKEEMQDILTYPNAEDNNNPIIRVVPAGRGKEIVAAHRAEARKRV
                     SSRNTMLMVLILGILLFAFFSGQILMGLFIVIILLMAFRTMMPRDEAMVPKLIVSNKP
                     DSKAPFVDATGSHAGALLGDVRHDPFQSGGLETPAHDRVEAGAIHRAHKGVLFIDEIN
                     TLELSSQQSLLTALQEGEFPITGQSDRSSGAMVRTEPVPCRFIMIAAGNLDAMQGMHP
                     ALRSRIRGYGYEVYMSETMDD"
     gene            1161..2006
                     /locus_tag="O0X12_00220"
     CDS             1161..2006
                     /locus_tag="O0X12_00220"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HJK52618.1"
                     /translation="MKVIYTEKSQLLASRVARHLGCKVAEVKYNTFPDGEQYVRVMDL
                     DDDMVIVASTVDSQSVLQAILMLDACEGKNTTLVLPYMGYARQDKRFNAGEPISARAL
                     ARALSEGADQIFTVNIHDPSVLSHFKCPAENLTIAQEIGKYIQTMNLEDPLVLAPDDG
                     AWEFAKGVASVGNWDCDHLDKTRLSGSEVKMAPKHLDAKGRDCIIVDDIIATGGSMAT
                     AAGMLKEQGASSVRAAGVHGVFASGGYVKLMQAGLADIASSDTIERACSKISASEVIA
                     NAVRR"
BASE COUNT          492 a          543 c          530 g          450 t
ORIGIN      
        1 atcatccatc gtctcgctca tgtacacctc gtaaccatat ccgcggatac ggctgcgaag
       61 tgccgggtgc ataccctgca ttgcatccag gtttcctgcc gcaatcataa tgaaccggca
      121 gggaacgggc tccgttctga ccattgcacc agacgatctg tcgctctgac cggtgatcgg
      181 gaactcaccc tcctgcagtg cagtcagcag actctgctgt gaggaaagct ccaatgtatt
      241 gatctcatcg atgaacagca cacctttgtg ggcgcggtga atagcacctg cctcaacacg
      301 atcgtgtgcc ggagtctcaa gacctcccga ctggaacgga tcatggcgga catcgccgag
      361 cagcgctcct gcgtgggaac cggttgcatc aacaaacgga gctttcgagt ccggtttgtt
      421 ggagacgatc agtttcggaa ccatcgcttc atcccgcggc atcatcgtcc ggaacgccat
      481 caggagaatg atcacgataa acaaacccat taagatctga ccgctgaaaa acgcaaacag
      541 gagaataccc aggatgagaa ccatcagcat cgtgtttctt gaagaaacac gcttgcgtgc
      601 ctctgcacgg tgggctgcaa caatctcctt accgcgtcct gccggaacca cacgaatgat
      661 cgggttgttg ttgtcctcgg cattcggata ggtgagaata tcctgcatct cctctttggg
      721 caagagctca gccattgcct tggcaagcat tgactttccc gtaccgggac tgccgatcat
      781 catcacatgg cggcgctggg tagccgcttt cttaataacc tcaaccgcgt gctcctgacc
      841 aatcacctga tcaatgagcg acggcggtat cgcaatgtca gctgttgtat taaactgaat
      901 gccaccgaag aactccggat cgaaactgtc cgatgcgtat gtcttcggga cagcaggctc
      961 tgcccgcgca ggagtctccg gctcctgcac tccggggctg ataaccggcg cagtccccaa
     1021 atctgcaccg ccagcgtgtc ctgccgtagt atctgtatca tccatagtta tttccttact
     1081 cgccttactc gagagtgtta atattgtgct tgatgatggt ttaagtagtt tcagtaaaag
     1141 atagaagaag gagagtactg atgaaagtta tctataccga aaaaagtcag ctgctggcct
     1201 caagggtggc ccgtcacctc ggatgcaaag ttgccgaggt caaatacaac acattccccg
     1261 atggagaaca gtatgtgcga gtgatggacc tcgatgacga catggttatc gttgcaagca
     1321 cggtggattc ccagtcagtt cttcaggcaa tcctgatgct ggatgcctgt gagggaaaga
     1381 acaccacact ggttcttccc tacatgggct atgcaagaca ggacaagcgg ttcaacgcgg
     1441 gtgaaccaat cagcgcacgg gcacttgcac gtgctctttc cgaaggcgca gatcagatct
     1501 ttaccgtaaa cattcacgat ccgtctgtac tcagccactt caaatgtcct gccgagaacc
     1561 tcaccatcgc gcaggagatc ggcaagtaca tccagacgat gaaccttgag gacccgctgg
     1621 tgcttgcacc ggatgacgga gcatgggagt ttgccaaagg tgttgcatcc gtcggcaact
     1681 gggactgcga ccatctcgac aaaacgagac tgtccggatc tgaagtgaag atggcaccaa
     1741 aacatctgga tgcaaaaggg cgcgactgca ttattgtgga tgacatcatt gcaacaggcg
     1801 gttccatggc tacagcggca ggaatgttaa aggagcaggg tgccagcagt gttcgtgcgg
     1861 caggcgttca cggtgtattt gcaagtggtg gctacgtaaa gctgatgcag gcagggcttg
     1921 cggatattgc ttcctctgac accattgaac gtgcatgcag caaaatctca gcatcggaag
     1981 tcattgcaaa cgcggtccgc cgataacttt catgc
//
LOCUS       DALTZZ010000014         2398 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_2577,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000014 DALTZZ010000000
VERSION     DALTZZ010000014.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2577"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="O0X12_00225"
     CDS             complement(<1..736)
                     /locus_tag="O0X12_00225"
                     /EC_number="5.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0046033 - AMP metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphate isomerase"
                     /protein_id="HJK52619.1"
                     /translation="MTLLQTAEQIQTMQIRGAGKIAREAVSALRDHAETLPHTGDASL
                     FIREMEHAASILLATRPTAVSLPNAIQMVMRDVRSARTEDDARRILREKADAFIWSSR
                     TAIDRIAAMGANHIPDGSVIMTHCNSKAALGCILEAKRQGKDIEVYATEVRPWNQGRL
                     TIKTLNDNEIPTTYIVDSAVRTMMKEVDLVIVGADAITVNGAVVNKIGTSQIALCANE
                     ARKNVIVTAETYKFAPRTILGELIQIE"
     gene            858..1688
                     /locus_tag="O0X12_00230"
     CDS             858..1688
                     /locus_tag="O0X12_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012108010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HJK52620.1"
                     /translation="MTVAAVFDSAGTLLRTYRSVVKVDEHKLADASVETTTLTFQDPD
                     RILVLLTLHSRHIMEKNPDALLSNYLTDEGVRFGISCGRRVIVSDVVGDILYGDKTAK
                     VSDMQAVIRDCWTTVSQRSDNFALNAGAIINLRTKMIEFTIASAGYPFPGVREMITQL
                     HSQGIAVFIASGDRTEKLVQIADQIGIPRNRVHGVATPISKAQIVQNLKNEYDVVIMT
                     GDGINDLSAMKAADVAILTVQQDGTRPEILFETADYVIENICEAARIIQEMAVNSETE
                     "
     gene            complement(1692..2369)
                     /gene="radB"
                     /locus_tag="O0X12_00235"
     CDS             complement(1692..2369)
                     /gene="radB"
                     /locus_tag="O0X12_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HJK52621.1"
                     /translation="MEEGLTKKISTNVLGFDRLLGGGLEPRMITQFVGEAGSGKSTLC
                     LVSAVAVLRAGGGVVYIDSEGFSVERFSQIAGENTEEFAKRIYIEEPMTFAEQGIMIA
                     GCESLLRAGKVGLIVVDSATALYRLEQMETKEALSMLSHQMMVLLALAKRFDVPVVIT
                     NQVFMDLERHKLSGLGGTALAHISKAIVRVEKHDGFRRAVVTKHRSQPEGVSWDFVIT
                     GDGVRDR"
BASE COUNT          661 a          628 c          552 g          557 t
ORIGIN      
        1 cctcaatctg gattagctca ccaagaatag tacgcggagc gaatttgtac gtctcggccg
       61 taacaatgac gtttttacgc gcttcatttg cacagagagc aatttgggaa gtcccaatct
      121 tatttacaac cgcaccgttt accgtgattg catcagcacc aacaataaca agatcaacct
      181 ctttcatcat agtgcggact gcagagtcaa cgatgtaagt tgtcggaatc tcattgtcat
      241 tgagggtttt gatggtcagt cttccctggt tccatggtct gacctcggtt gcatacacct
      301 caatatcctt tccctggcgt ttcgcctcaa ggatacagcc aagggcagcc ttggagttac
      361 agtgcgtcat aataacactg ccatccggaa tatggtttgc acccattgcc gcaatacgat
      421 cgatcgcagt acgtgacgac cagataaacg cgtccgcctt ttcacgaagg atacgccggg
      481 cgtcatcctc cgttctggcg ctgcgtacgt cacgcattac catctgaata gcattcggga
      541 gggaaactgc agttggtcgt gttgcaagaa gaatgctggc cgcatgttcc atttcccgga
      601 taaacaaaga tgcatcacca gtatgcggaa gagtctcggc atgatcacga agggcagata
      661 ccgcttcgcg tgcaattttt ccagcgccgc ggatctgcat ggtctggatc tgctcagctg
      721 tctggaggag agtcatatgt aaaaatgggg gtgctacgtt atatagctgc gggtatcgga
      781 aacaagactg ttccgcgtaa aatgattcaa gaacgcagac aaaagaatat atgagcattt
      841 acaacggatg tagcagtatg acggttgcag cggtttttga cagtgcgggc actctcctca
      901 ggacataccg gagcgtggta aaagttgatg aacacaaact tgcggatgca agtgttgaaa
      961 cgacaacact gaccttccag gaccctgaca ggatactggt tctccttacc ctgcactccc
     1021 gtcacatcat ggaaaaaaat ccagatgccc tgctctctaa ctacctaaca gacgaagggg
     1081 tccgttttgg catcagctgt ggacggaggg taatcgtatc agatgtcgta ggagacattc
     1141 tgtatggaga caaaaccgca aaagtatcag acatgcaggc agtaattcgg gactgctgga
     1201 caacagtatc tcagagatct gataactttg ccctcaatgc cggtgcaatc atcaacttgc
     1261 ggacaaaaat gattgagttc actattgcgt ccgcaggata cccatttccc ggagtgcggg
     1321 aaatgatcac gcaactccat agtcagggga ttgccgtgtt tatcgcatca ggagacagaa
     1381 cagaaaagct cgtgcagata gccgatcaga tcggtatccc gcgaaaccga gtacatggtg
     1441 tggcaacgcc tatcagcaaa gcccagattg tccagaacct gaaaaatgag tatgatgttg
     1501 tgatcatgac aggtgatgga atcaacgatc tctcagcaat gaaagcagca gatgttgcaa
     1561 tcctcacagt acagcaggat ggaacacggc cggagattct gtttgaaacc gcggactatg
     1621 tgattgaaaa catctgtgaa gcagcacgga taattcagga aatggcagtg aacagtgaaa
     1681 cagagtaggc gttacctatc cctaacacca tccccggtaa tgacaaagtc ccacgaaact
     1741 ccctccggtt gcgaacgatg tttagtcacc actgcacgcc ggaacccatc atgcttctct
     1801 acccgcacaa ttgctttcga aatatgtgca agagcagtcc cgccaagtcc gctcagtttg
     1861 tgccgctcca gatccataaa cacctgattt gtaatcacca ccggaacatc aaaacgtttt
     1921 gcaagagcaa gtaaaaccat catctggtga gaaagcatgg aaagtgcttc tttggtctcc
     1981 atctgctcca gacgatacaa agcagttgcc gagtcaacca cgataagccc gacttttccc
     2041 gcacgcagaa gagactcaca cccggcaatc atgatcccct gctccgcaaa tgtcatcggc
     2101 tcttcaatat agatacgctt tgcaaactcc tcggtgttct ctccggcaat ctgggagaaa
     2161 cgttccaccg aaaatccctc ggaatcaata tagaccaccc cacctccggc acgcagtacc
     2221 gcaaccgcag agacaagaca aagagtactc tttccggaac ctgcctctcc cacaaactgt
     2281 gtaatcatcc gcggctcaag tccgccaccc agaagtctgt caaatcccag aacattcgtg
     2341 gaaatttttt tcgttaatcc ttcttccatg cctcctcctc aatcacgcgc ttcatgtc
//
LOCUS       DALTZZ010000015         8411 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_2829,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000015 DALTZZ010000000
VERSION     DALTZZ010000015.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 8411)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8411)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8411
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2829"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..761
                     /locus_tag="O0X12_00240"
     CDS             <1..761
                     /locus_tag="O0X12_00240"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314367.1"
                     /note="Part of a complex that catalyzes the formation of
                     methyl-coenzyme M and tetrahydromethanopterin from
                     coenzyme M and methyl-tetrahydromethanopterin. This is an
                     energy-conserving, sodium-ion translocating step. MtrH
                     catalyzes the transfer of the methyl group from
                     methyl-tetrahydromethanopterin to the corrinoid prosthetic
                     group of mtrA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H"
                     /protein_id="HJK52622.1"
                     /translation="FQWFDSIDNKTAFLMDSSAPEALVHATKYVTEVGLADRAIYNSI
                     NGSIQPENIQALAESDVDAAIVLAFNPGDGSVAGREKVLSEGGVAGQAKGMLQIAEEC
                     GIKRPILDTAATPLGLGSAGSFREILACKAIHGLPTGGAYHNMTVSWTWLKRWRKNIH
                     EQYKGKDILAEQMFHHHYGGIEGVRQIAWSSPDIGCNIMAMTLGADLIMFGPIENMEG
                     IATAAAFSDIVLSEALTELGGEVQDHNGPLYKLV"
     gene            932..3094
                     /locus_tag="O0X12_00245"
     CDS             932..3094
                     /locus_tag="O0X12_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJK52623.1"
                     /translation="MRVSEGNLTQQYYCDYLEILAEEILSASVTEEVRRGVHENIVFR
                     NLFLHRMGVDGGFHARVISAILHQPDDSRLLMDLLMASKQVDTDVLPVEEWLKQEYAS
                     AVDKQNSARMVAVEALLRSYISGTNQAAEIIASAGDSHPQSWWQLETRRITIAEVANP
                     DMYFGVGRRPFWHEFPLDRYAAVVAGVPDLLPHEIYDCVVCIAGAWFSSLTREEMITW
                     LNMPQPTLSEWETLCAPLLKCDTPDAALSTANWLYASGNNDPAMDLYANITLAFPGTP
                     AEISSFELIGTILRKLGDYDNAFEAYKNAFIASRGAGGYQTAIGLKNLCEVGEDLGED
                     MSDYYTRIAGIADMLPVSEKLRLYLDLAASCRRRHAYEEEYRYLEQVLDAEDEDDEDL
                     VAAAMSRLSEMNMSLSADGTPDTLLLAAKDEESESSVSVTRGTAAYFGFDPASALMWY
                     ERSGSSHISSLQFAAAMAAGVDAEEYAKTPSQHAVVLAAEGSPVTEIIRELNVAATDA
                     WRRGEDAVQGFEQIIPYLTPDQMCTVFVEVTARSTRDDERAVVCSAAAKALLSAGYVN
                     EARTMLRAALRANPGREMRSRLFAELGWLEYESGVYSASVDAYDGALKINDQFPAAWA
                     GRARALACMGKYDEALVAAGYAVVQNPSNGSYQHLRAALETASQSPVDPATDLLFVLP
                     EPGYLQAAAARYSVKKTNSCPPSVWDVSNVEDVLPMRF"
     gene            3100..5211
                     /locus_tag="O0X12_00250"
     CDS             3100..5211
                     /locus_tag="O0X12_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52624.1"
                     /translation="MKQVVALLLVALSLVILFAVFTGGGNNEPDPLPPITNVTKYNGT
                     IGVILPSNSTIGEQVRNGIDTAYLLQDTKTRPSLVYLDSEDLTSLPALDAVITATDEI
                     SETWDAQTDMRGTVHVAVASPGYAASAPDGVVTFSTPPYYEIESMQSLLQQHDVIGVL
                     GPDNAYTAAYIHDLKDATGSRIISGIVSSSTGEIVSLRSLLVQNPSLLIITGGEDVPN
                     IVAKAREFGLTGPIMVSSWIGEDATIANDSRMEGVYTAKRMSDISSHPFYNVYQARFG
                     TTGSIYAAEGYDAMMTLCRLVPQSNGSTQYVSSWYLGKNYEGAAGSYEFDDDGCGRIL
                     MQIAQFQSGSVVPVDTYVRPPSEVIIGVYGNDEVVRGAVAGAELVNSASTVGLPGAST
                     SGISGLYGAKVSILPLVAGYPMPENVTAVIGDLSGVVTDLPGVHTVSKNWVPDGWSVS
                     LAPDANAGFSLFLDSVDERRGYGTPLNNVTILHPDNTQPPAGTISTLENRGYHVSVVP
                     YSLPLSETEAVALMAGLSCTGQVLVSLPDSAADLTILLAGARQTGSIPAFWYVSGDVI
                     LGDPALVQSTLIYDSLIGSDIWSSELGKSKPLVREVSLFYSSVYPGKSMTGVSARSFE
                     AVVMLADAMSVSGSTAPSSVGSALKTLNYSADQSIFFGDGVSFDASGVAVGPETMLLQ
                     VQSGTTRIISSSADLRLMTKD"
     gene            5310..7769
                     /locus_tag="O0X12_00255"
     CDS             5310..7769
                     /locus_tag="O0X12_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HJK52625.1"
                     /translation="MTIRYLRFLVGGMHCSACSAHLESTLKALPGILEVTVNLSTATV
                     SANIVTDLVTPAKIDEAVRSAGFSVNSVPVSFIVTPADDVSISAAAKRTLQELSGVQD
                     VSESGDLVTILYHPLLADPSQFVSVLKKAGYSSVTVNSGFDDLFYIPEHEKDELSGLR
                     NRTILGFIGSAVLMGLMLFVHPLFAEDMFLMDILMLVIATPILVVCAYPIFQQGISQL
                     RGGMLGMEVMYMLGITTSYIAGLGAVAGYLPSPDFLLFETAVMLTAFLGLGRFLETRA
                     RGRTSDAILALLHLRPPVATVIRDGVAQEVRVGELVAGDTVLVKTGGQIPSDGIVLEN
                     PVTVNESAITGESVPVVKMPGTSVIGGTVVSQGVLTMEVEKVGADTVLAGIIKMVREA
                     QETKPPVQRLADTAVRYFIPFVLSVAIIAFVYWFFFAGETLLIALSSFIAVIVVACPC
                     ALGLATPTAVTVGIGRAASLGILIRNGEVLERAGKLKAVAVDKTGTLTVGSPTVSDVV
                     PAPGTSEEDLLAIAASLGRKSLHPLDSAIVAEADKRGIADLAAARVETVPGRGMTARV
                     KRETVVMGSAEFMEDMSIIADDFVSSAAEKFRAAGSSVVFVAVAGKVVGAVGISDALR
                     PDAVRAVAEFENMGLPVTMLTGDNARAAEVVAGLAGISHVEAGLMPDEKRTIIQHMQE
                     EGKAVVFVGDGINDTPAMTQADVGIAVGSGTDAAVASGGIVLLRSDLIGAVAAVQLAR
                     KVFSRIRMNLFWAFAYNLFLIPLAAGVLYPFTHVMFRPEYAAAAMVVSSVTVVSLSLL
                     LRRYTPPALRNNNVSPIENAS"
     gene            complement(7902..>8411)
                     /locus_tag="O0X12_00260"
     CDS             complement(7902..>8411)
                     /locus_tag="O0X12_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52626.1"
                     /translation="VLDICEETFPHEFTIIPLPGHYFDMIGVVTPDKVFFCADSLSGE
                     HILEKYHVAVLYDVEAYLITLETVAITDAELFVPAHAEPVTDIRELARMNAMKVEEIA
                     DQILELCGAGASFEDILAGLFVRYNLSMDCNQYVLVGSTVRSYLSYLHGKGLIDIMFA
                     DNRMLWARK"
BASE COUNT         1893 a         1866 c         2300 g         2352 t
ORIGIN      
        1 acttccagtg gttcgacagc atcgacaaca agactgcatt cctgatggac tcatctgccc
       61 ctgaggcact cgttcacgca acgaagtacg ttacggaagt tggtcttgca gaccgtgcaa
      121 tctacaactc catcaatggt tccatccaac cggagaacat ccaggcactt gccgagtctg
      181 acgttgacgc tgcaattgtt cttgcattca acccgggaga cggttccgtt gcaggccgtg
      241 agaaggtact ttctgagggt ggtgttgcag gtcaggcaaa gggtatgctt cagattgcag
      301 aagagtgcgg tatcaaacgc ccaattctcg acactgcagc aactccgctc ggtcttggct
      361 ctgccggttc gttccgtgag atccttgcat gcaaggcaat ccatggtctg ccgaccggtg
      421 gtgcatacca caacatgact gtgtcctgga cctggctgaa acgctggaga aagaacatcc
      481 acgagcagta caagggtaag gacatccttg cagaacagat gttccaccac cactatggcg
      541 gtatcgaggg cgttcgccag attgcctggt cttccccaga tattggatgt aacattatgg
      601 caatgaccct tggtgcagat ctgatcatgt ttggtccgat tgagaacatg gaaggtattg
      661 caaccgcagc agccttctcc gacatcgttc tgtccgaggc tcttaccgag ctcggcggcg
      721 aggttcagga tcacaacggc ccgctgtaca agctggtcta atcttcctga ccaataaaat
      781 caattttttc tgtagtgtgg gaaaaaccgg gtttatcctg gttatcacct gcagtaacgg
      841 tttatcccga ttcggttttg tattcttttt tcactttctt agatctatca gaaggcatac
      901 atagggtaaa cgtacaaaac actacataag tatgcgagtc agcgagggga atctgaccca
      961 acaatattat tgtgattatc tggagattct tgcagaagag atattatctg cgtccgtaac
     1021 tgaggaggtg cggagaggtg tgcatgaaaa tattgtgttc cgcaatttgt ttctccaccg
     1081 aatgggtgta gatggaggtt tccatgcacg agtgatctct gcaatcctgc atcaaccaga
     1141 tgatagccga cttctgatgg atcttcttat ggcatcaaag caggttgata ccgatgtgct
     1201 accggtcgaa gagtggctga agcaggagta cgcatcagca gttgataaac agaacagtgc
     1261 acggatggta gcagttgagg cactgctgcg atcctatatt tcgggaacaa atcaggcagc
     1321 cgagatcatt gcatctgccg gggattcaca tccccagagt tggtggcaac ttgaaacacg
     1381 gcgtatcaca attgctgagg ttgcaaatcc tgacatgtac ttcggtgttg ggcgccgtcc
     1441 gttctggcat gaatttcccc ttgaccggta cgcggcagtt gttgcaggcg tacctgatct
     1501 tcttccccat gagatttatg actgtgttgt ctgcattgca ggggcatggt tctcctctct
     1561 tacgcgtgag gaaatgatca catggctgaa tatgccgcag cctacattgt ctgagtggga
     1621 aacactctgt gctccgcttt tgaagtgtga tacccccgat gcagcacttt ctactgcaaa
     1681 ctggttgtat gcttcaggaa acaatgatcc ggcaatggat ctctatgcaa atattactct
     1741 tgccttcccc ggaacccccg ctgagatatc gtcatttgag ctgataggta ccattctgcg
     1801 aaaattagga gattatgata atgcgtttga ggcctacaag aacgctttca ttgcatcacg
     1861 tggtgcaggt ggttaccaga ctgcaattgg tttgaaaaat ctgtgtgagg ttggtgagga
     1921 cctcggtgaa gatatgtcgg attactatac gcggattgcg ggaattgcgg atatgttgcc
     1981 cgtttctgaa aaattgcgtc tgtatctgga tcttgctgca tcctgcagga gacgtcatgc
     2041 gtatgaggag gagtacaggt atcttgagca ggtgcttgat gcagaggatg aggatgatga
     2101 ggatttggtg gctgctgcaa tgtcacggtt gtcagaaatg aatatgtccc tttcagcaga
     2161 tggtaccccg gacactcttt tgcttgcagc aaaagatgaa gagtccgagt cttctgtttc
     2221 ggttacaagg ggaactgccg cttatttcgg atttgatcct gccagtgcac ttatgtggta
     2281 tgagcggtcc ggttcttcac atatctcatc gctacagttc gctgcagcaa tggctgccgg
     2341 tgttgacgcg gaggagtatg cgaaaacccc atcccaacat gcggtggttc ttgcagcaga
     2401 aggttcacca gtgacggaga ttattcgtga attgaatgtg gcggcaactg atgcgtggcg
     2461 gaggggtgaa gatgccgtac aggggtttga acagataatt ccatatctca ctcccgatca
     2521 gatgtgtact gtttttgtcg aggtaacagc acgttccacc cgcgatgatg aacgtgctgt
     2581 tgtgtgttcc gcggcagcga aggcattact ttccgcagga tatgtgaatg aggcacggac
     2641 gatgctgcgt gctgcactcc gggcaaatcc cgggcgtgag atgagatccc ggttgtttgc
     2701 agaacttgga tggctggagt atgagtccgg agtatattct gcatcggttg atgcatatga
     2761 cggggcacta aagatcaacg atcagttccc tgcggcatgg gctggccgtg cccgggctct
     2821 tgcgtgtatg ggaaaatatg atgaggcgct tgtagctgct ggttatgctg ttgtgcagaa
     2881 cccctcaaat ggttcatatc agcatcttcg tgcagctcta gagactgctt cacagtcccc
     2941 cgtagatcct gcgacagatc tgctgtttgt cctgccggaa ccaggttatc tgcaggcagc
     3001 tgcagcccgg tattctgtga aaaagacaaa ctcctgtccg ccatcagtgt gggatgtgtc
     3061 gaatgttgag gatgttcttc cgatgaggtt ttagttccaa tgaaacaggt cgttgcgctt
     3121 cttctggttg ctctctcctt ggtaattttg tttgctgttt ttaccggggg tggcaacaat
     3181 gagcctgatc cgcttccgcc aatcaccaat gtaacgaaat ataatggtac aatcggtgtg
     3241 atcctgccga gtaattctac aattggtgag caggtgcgaa atggaattga taccgcttac
     3301 ctcttgcagg acacaaaaac ccgtccatcc ctcgtgtatc tggatagtga agatcttacg
     3361 tctcttcctg ctctcgacgc tgtgatcact gctactgacg agatcagtga aacgtgggat
     3421 gcacagactg atatgcgggg aacagtgcat gtggctgtag cctcaccagg gtatgcagca
     3481 agtgctcctg acggggtggt tacgttcagt accccaccat attatgaaat agaatctatg
     3541 cagtcgcttt tgcagcagca tgatgttatc ggggtactcg ggccggacaa cgcctacact
     3601 gctgcataca ttcatgattt gaaagatgcg acaggcagca ggatcatttc aggaatcgtt
     3661 tcttcatcga caggagagat tgtatcactt cgttctctcc tagtccagaa tccaagtctc
     3721 ctaatcatca ctggtggtga ggacgttccc aatattgttg caaaagcccg tgagtttgga
     3781 ctgaccggcc cgataatggt ctcttcatgg attggtgagg atgcaacaat tgcgaatgac
     3841 tcccgaatgg agggtgtcta tacggcaaaa cgtatgtccg acatctcttc acatccattt
     3901 tataatgtgt atcaggcacg gtttggtacg accggatcga tttatgcggc agagggatat
     3961 gatgcgatga tgacactctg ccggcttgtt ccgcagagta acgggagtac tcagtatgtt
     4021 tccagttggt acctgggaaa gaactatgag ggggctgccg gttcgtatga gttcgatgat
     4081 gatgggtgtg gccggattct gatgcagatt gcacagttcc agtcaggttc tgtggtccca
     4141 gtggatacat atgtgcgtcc tccgtctgag gttatcattg gggtgtacgg gaacgatgaa
     4201 gtggtccgtg gagcggttgc gggtgctgag ttagttaaca gtgcaagtac tgttggacta
     4261 ccgggagcgt caacaagtgg tatttcggga ttgtatggtg cgaaagtatc tatcctcccg
     4321 cttgtagcgg gataccccat gccagagaat gtaactgcgg ttataggaga tcttagtggc
     4381 gtggttacgg atcttcctgg tgtacatact gtttccaaga attgggttcc ggacggatgg
     4441 tccgtgtcgc ttgctcctga tgccaatgcg ggtttttcct tgttcttgga tagtgttgat
     4501 gagaggagag ggtatggtac gcccctcaac aatgttacaa tattacatcc agacaacacg
     4561 cagcctcccg ctggaacaat ctccacactg gaaaaccgcg gttaccatgt ttcggtggtg
     4621 ccctattccc ttccgctgag tgagacggaa gctgttgcac tgatggcggg attatcctgt
     4681 accgggcagg ttctagtgag tttacctgat agtgcagcgg atttgaccat tcttcttgct
     4741 ggtgcccgac agacgggttc aatccctgct ttctggtatg tttcagggga tgtgattctt
     4801 ggtgatcctg ccttggtaca aagtacactg atttatgatt ctcttattgg ttcagatatc
     4861 tggtcctcag aacttggaaa gtcaaaaccg ctggttcggg aggtgtcact gttctattca
     4921 agtgtgtatc ccggaaagtc gatgacaggt gtatctgcca gatcatttga ggcggtggtg
     4981 atgcttgccg atgctatgag tgtgtccgga tccaccgctc cgtcgtcagt tggttctgct
     5041 ttgaaaaccc tcaactactc tgcagaccag agtattttct ttggtgacgg tgtctcattt
     5101 gatgcttctg gtgttgctgt tggtccggag acgatgctct tacaggtcca gagtggtact
     5161 acccgtatca tcagcagttc tgcagatctt cgtctgatga caaaagacta acctgatttt
     5221 ttctttttgg tgttgtctac aatatttcct gttatttttc gtgaacccta acagagtcta
     5281 tttgtgtagt gaggaataac atatggttca tgacgattcg ttacctccga tttctggttg
     5341 gcggcatgca ttgcagcgca tgctcggctc atcttgaatc cacactaaag gccctccccg
     5401 gtattttgga ggtcacggtc aatctttcca cggcaactgt gagcgcaaat atcgttacag
     5461 atctggtaac cccggcaaag atagatgaag ctgtccgatc ggctgggttc tcggtgaact
     5521 cagtgccggt ctcgtttatt gtaactcctg cggatgatgt ttccatatca gctgctgcaa
     5581 aacgtacact acaggaactt tctggcgtgc aggatgtttc tgagagcggg gatctggtaa
     5641 cgatcttgta tcatccgttg cttgcggacc catcacagtt tgtttcagtt ttgaaaaagg
     5701 caggttattc ttcggttact gtgaacagcg ggtttgatga tctgttctat atccctgagc
     5761 atgagaagga cgaactgtct ggtttgcgaa acagaacgat tctcggattt atcggttcag
     5821 cagttttaat ggggctgatg ctatttgttc atcctctgtt tgcggaagat atgtttctga
     5881 tggatatttt gatgctggtt attgcaacac cgattctggt tgtatgtgcg tatccgatct
     5941 tccaacaggg gatctcccag cttcgcggag gtatgctcgg tatggaggtt atgtatatgc
     6001 tgggtattac gacgtcgtat attgcgggtc ttggtgctgt tgcaggatat ttaccatcac
     6061 ccgactttct gctgtttgaa actgctgtga tgcttaccgc atttttagga cttggccgat
     6121 ttctcgaaac acgggcgcgg gggcgaacat cggatgcgat tcttgcactg ctgcatctcc
     6181 gtcctccggt tgcaacggtc attcgtgatg gtgtagccca ggaggtgcga gttggggaac
     6241 ttgtcgcagg ggacacagtt ctggtaaaga caggtggtca gattccatcg gatggtatag
     6301 ttcttgaaaa cccagttacg gtgaatgagt ctgcaattac tggtgagtcc gtgccggtgg
     6361 taaaaatgcc gggaacgagc gttattggtg gtacagtggt gtcacaggga gttctcacga
     6421 tggaggtgga aaaggtgggg gcagacactg tacttgcagg gatcattaaa atggtgcggg
     6481 aagcacagga gacaaaacct cctgtacagc ggcttgcaga cacagccgtc cggtacttta
     6541 ttccgtttgt tctaagtgta gcaattattg cattcgtgta ctggtttttc tttgccggcg
     6601 agacactgtt gattgcattg tcgagtttca ttgcagtaat tgtggttgcc tgtccctgtg
     6661 cactgggact tgcaacaccg accgcggtga ctgtaggaat tggtcgggca gcaagtcttg
     6721 gaattttgat ccggaatggt gaggtactgg agcgtgcagg gaaactgaaa gccgttgcgg
     6781 tggataagac aggtacacta acagtaggtt ctccaacagt atctgacgta gtacctgccc
     6841 caggtacaag cgaagaagat ctccttgcga tcgccgcatc tcttgggcgg aaatctttgc
     6901 atccacttga ctcggcgatt gttgcagagg cggataaacg cggtattgcg gatcttgcgg
     6961 cggcacgggt ggaaacggtt cccggcagag gaatgacggc aagggtgaaa cgtgaaactg
     7021 tggtgatggg atcggccgag ttcatggagg atatgagtat catcgcggat gattttgtat
     7081 catctgctgc ggaaaaattc cgtgcagcgg gatcgtcggt ggtcttcgtt gctgtcgccg
     7141 gaaaggttgt gggagctgtt gggatctccg atgcactccg acctgacgcc gtccgtgccg
     7201 ttgcggagtt tgagaatatg ggtcttccgg taacgatgct gacaggggat aatgcacgtg
     7261 ctgctgaggt tgttgctggc cttgctggca tctctcatgt tgaagcaggt ctgatgccgg
     7321 atgagaaacg tacgattatc cagcacatgc aagaagaggg aaaggcagtg gtgttcgtgg
     7381 gtgatggaat taatgatact ccggcgatga cgcaggctga tgtaggtatc gctgtgggca
     7441 gcggaacgga cgcggcggtt gcatctgggg gaattgtttt acttcgttcg gatctgattg
     7501 gtgcagttgc ggcagtccag cttgcacgca aggtattttc gagaatccga atgaatctgt
     7561 tctgggcatt cgcatacaat ctgtttctga tcccacttgc ggcaggtgta ttgtatccgt
     7621 ttacgcatgt gatgttccgg ccggaatacg cagcggcagc gatggtggtg agttcggtaa
     7681 cggtggtaag tctctcactg cttctacgga gatatacacc gcctgctttg cgaaacaaca
     7741 atgtttcgcc gattgagaat gcatcgtgag agtgttgttc agtacccatt ctctctattt
     7801 tgggtcttta gtgagttttg aatagccacg aagatccgga atttattgta gcaatgaaat
     7861 atttcttcag gatttctttt tcctggtttt ctgtatgctc cttactttct tgcccacaac
     7921 atccggttgt ctgcaaacat tatgtcaatg agtccttttc catgcagata agagaggtac
     7981 gaccgtactg tactcccgac cagtacatac tggttgcagt ccatcgagag attgtaccgt
     8041 acaaataatc ccgctaatat atcctcaaat gatgcacctg ctccgcacaa ctcaagtatc
     8101 tgatctgcaa tttcctctac cttcatggcg ttcattcgtg cgagttccct gatatctgtt
     8161 actggttcgg catgtgcagg gacaaatagt tcggcatccg taattgccac cgtttccagt
     8221 gttataagat atgcctctac atcgtagaga acagcgacat ggtacttctc tagtatgtgc
     8281 tcccctgata aactgtctgc acagaaaaac accttgtccg gggtcacaac tccgatcata
     8341 tcaaaataat gtcccggcag ggggattatc gtgaactcat gcggaaaggt ttcttcgcat
     8401 atatccagta c
//
LOCUS       DALTZZ010000016         2486 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_2909,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000016 DALTZZ010000000
VERSION     DALTZZ010000016.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2909"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /gene="dnaG"
                     /locus_tag="O0X12_00265"
     CDS             complement(<1..805)
                     /gene="dnaG"
                     /locus_tag="O0X12_00265"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase DnaG"
                     /protein_id="HJK52627.1"
                     /translation="MYSPDTTKYLIHIHIETEGVVEKPDVVGAIFGQTEGLLGEDLDL
                     RDLQRSGRVGRIDVQITSKHGKTSGELYIASSLDRAETAILAASLETIERVGPCVSTI
                     KVQGIEALRAIKRRQIVDRAKELLLESFDEVGISTNEILAEVRESIRVEKVSSIGEER
                     LPAGPNVLDSDAIIIVEGRADVLNLLKCGIKNTVAVEGTKVPQIVADLSVKKNATVFV
                     DGDRGGDLILRELLQVADIDYVAFSPRGRSVEDMSRKEIVKSLRNKVPAD"
     gene            complement(924..1448)
                     /locus_tag="O0X12_00270"
     CDS             complement(924..1448)
                     /locus_tag="O0X12_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52628.1"
                     /translation="MGKADAASSVSGSILLFLLAASLCTLTAASIAGMLILPDDTAGP
                     VPEVIAIGEIHHENPAGIVTYAGRLYLIHVGTEPLDLAGHRAEVYVNGIKQRVVIETL
                     KGSDFIPTHHYGVGQLAGPGVRGEKWYPGQWAWVNIGDEMILEEDEVVFDVIRRSDGK
                     IVSRSRRYAPKIAR"
     gene            complement(1717..2208)
                     /locus_tag="O0X12_00275"
     CDS             complement(1717..2208)
                     /locus_tag="O0X12_00275"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HJK52629.1"
                     /translation="MTTTDIPGTEKIAVAQDGDSVSPHFGHCESYAIFQIENGAAVRL
                     EDMENPGHEPGKLPLHLAGTGITTVLAGGIGQRALAYLQENGVTVIRGVSGDVNAAAA
                     AYAAGTLVSGEDSCGCHGDGEHHGHHHHHGNGSCGCHEEGEHHHHMHHHGHGSCGCHG
                     HSE"
     gene            complement(2289..>2486)
                     /locus_tag="O0X12_00280"
     CDS             complement(2289..>2486)
                     /locus_tag="O0X12_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HJK52630.1"
                     /translation="DLAVYADAPVKIAAENLHSKCGWTPYEGMNGLFPETTVVGGTAV
                     WHKGEFVRGQPLWLAGAGRKA"
BASE COUNT          522 a          728 c          619 g          617 t
ORIGIN      
        1 catctgcagg aaccttgttt ctcagggatt ttacaatctc tttgcgggac atgtcctcca
       61 cgcttcttcc gcggggagag aaggcgacat agtcgatgtc tgcaacctgc agcagctcgc
      121 ggaggatcag atctcctccc cggtcgccgt ccacgaacac tgtggcgttc ttcttgacac
      181 tgaggtctgc cacaatctgg ggaaccttgg ttccctcaac cgcaacggtg tttttaattc
      241 cgcacttcag caggttcagc acatccgccc gtccttccac aatgatgatg gcgtccgagt
      301 ccagtacatt gggtcctgcc ggcagccgtt cctctccgat ggaggacacc ttctccacgc
      361 ggatggactc ccggacctct gcgagaatct cgttggtcga gattccgacc tcgtcaaacg
      421 actcaagcag cagctccttt gcccggtcca caatctgccg ccgtttaatt gcccgcagag
      481 cctcgatacc ctgcaccttg atcgtcgaga cacagggacc aacccgctcg atcgtctcca
      541 gagatgccgc aagaattgcg gtctctgcac gatcaaggga ggatgcaatg tagagttcac
      601 cggatgtctt tccgtgttta ctggtaatct gtacgtcgat gcgtccgacc ctgccgctcc
      661 gctgcaggtc acgcaaatcc agatcttcgc ctaataatcc ttcggtttgg ccgaaaattg
      721 cgccgactac gtcgggtttt tcgaccaccc cctctgtttc gatatgaatg tgaataagat
      781 actttgtagt gtcaggtgag tacatgtaag aaagcctcca tggttggcgt aaggaatgta
      841 ataggtaacc atagacagat accatatgta ctctttacgc ttataaacta aacgaccaca
      901 aagttttagg taaggtattc tgattacctc gcaatttttg gagcgtatct tctcgatcgc
      961 gagacaattt ttccgtcgga tcttcggata acatcaaaaa caacctcgtc ttcctccaaa
     1021 atcatctcat ctccgatgtt cacccaggcc cactgtccgg gataccattt ctccccccgg
     1081 acccctggtc ctgcaagctg tccgacgccg tagtgatggg tcggaatgaa atcacttcct
     1141 ttcagggtct caatcaccac ccgctgcttt attccgttca catacacctc ggcacggtgt
     1201 ccggcaagat cgagcggttc tgttcccaca tgtatcagat acaggcggcc tgcataggtc
     1261 acaattccgg caggattttc gtgatgaatc tctccgattg caatcacctc gggaaccggt
     1321 ccggctgtat cgtccggcag gatcagcata cctgcaatcg atgcggcagt tagggtacag
     1381 agggatgcgg caagcagaaa caacagtatg cttccagaaa ctgacgaggc agcatccgcc
     1441 ttgcccatat gcaggagttg gcgtgtgagg gataataaga gtgtcccccg tcgttaaccg
     1501 agtccattca tcggtggctg ttgccgtccc ttatggatct ttgaccggac ggggggtgcc
     1561 tgtagcatgt cgtgttgtcc cgtggatcat cctccgcgac cgcgtatatg tcacccgggg
     1621 gcctaacaca tgccgagtgt cgacacacct cactatacgg tctcaactgc aataaatcct
     1681 gcacaccaaa aaagagaggg gatgcctgcc ttccggttac tcgctgtgtc cgtggcagcc
     1741 gcaggagccg tgtccatggt ggtgcatgtg atggtgatgc tctccttctt catggcatcc
     1801 gcacgaaccg ttgccgtgat gatggtggtg tccgtggtgt tccccgtcgc cgtgacatcc
     1861 gcaggagtct tcaccagaga cgagtgttcc tgccgcatac gcagccgccg ctgcgttcac
     1921 atctcctgag acaccgcgaa tgacagtgac tccgttctcc tggagatatg ccagtgcccg
     1981 ctggccgatt cctccggcaa gtactgtggt aattcctgtc ccggcaagat gcaggggaag
     2041 ttttcccggt tcatggccag ggttctccat atcttccagc cggactgctg cgccgttttc
     2101 tatctgaaat attgcgtaac tctcacagtg gccgaagtgc ggggagacac tgtcgccgtc
     2161 ctgggcgacg gcaatcttct cagtgccggg gatatctgtc gttgtcatat attacacata
     2221 tgcgcataat tataaaatat ttttctgatc acggcattgc gtgagctctg ttctctccaa
     2281 aaaaaagttc aggcttttct tccggcgcct gcaagccaga gcggctgtcc gcggacaaac
     2341 tcgcctttgt gccagaccgc tgtaccgccg acaaccgttg tctccgggaa cagcccgttc
     2401 attccctcat acggtgtcca gccgcatttg ctgtgcagat tctctgccgc aatcttcacc
     2461 ggagcatccg catacaccgc aagatc
//
LOCUS       DALTZZ010000017         1721 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_3074,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000017 DALTZZ010000000
VERSION     DALTZZ010000017.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3074"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(6..1013)
                     /locus_tag="O0X12_00285"
     CDS             complement(6..1013)
                     /locus_tag="O0X12_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52631.1"
                     /translation="MSSVPKLLIGLSCPGCGGTIEVTEGSRLAFCPYCGSALAITADD
                     AGVTRLMYKMEITEAEAERSARKWFGAFPKARDLSATAVITEIFPMYLPFWRVVGTGK
                     GVACGYSTHTDKDGHTQRTYHEDSSDVEYLWSVIACQTGDLGIATPPELKGTLLPYTD
                     GDIPVFEITSSRDEAYDQADTDIRMEAHRRAASGIETLTFSKTFCIPREFSLLYYPYW
                     IVRYQYRERDYFVVLDGVSGDPVSGRAPGDQTYQALAAGLGAGFGGLFAGLGVAYAVF
                     SNSEYALAGLSGLVIGLILVLAGYWMFRFGSEVTEGKLTGGVTFSMKGFGQKTVREIE
                     R"
     gene            complement(1010..>1721)
                     /locus_tag="O0X12_00290"
     CDS             complement(1010..>1721)
                     /locus_tag="O0X12_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52632.1"
                     /translation="GKQYTICENCGTVIYIDRSSVIFHYIMPFILDEDKARAVFRRWC
                     AGPSLAKDLEREAQITSVEKIYFPVFLFRRVVDGPEKSIIKPAKGTTLPGLQSQVIPP
                     GDIQVFDSTVSTAGAEVIHPEISVETYLADLPGTAKEQALLYLPFFVFRYRYQGVEYI
                     SVMGGTSGRVHTSGFPGRSAAPYALVVGGGFGLAFIGGILGFVTTPLFYVLVFAGAAL
                     AVLTGRAVVRTPDGGESK"
BASE COUNT          445 a          477 c          459 g          340 t
ORIGIN      
        1 ccatattatc gttcaatctc ccggacagtt ttctgtccaa atcctttcat ggaaaatgtc
       61 actcctccgg tgagttttcc ttccgtaacc tctgatccga accggaacat ccagtacccg
      121 gcaagaacaa ggatgagacc aatgacaaga ccggagagac cggcaagtgc atactcggag
      181 ttggaaaaca cagcataggc aacaccaaga cccgcaaaca gacctccgaa ccctgcacca
      241 agacctgcgg caagtgcctg atacgtctga tctcccggtg cacgcccgga aacaggatct
      301 cccgagacgc cgtcaaggac aacaaagtaa tcgcgttcgc ggtactggta gcggacaatc
      361 cagtacgggt aatagaggag cgaaaactca cgggggatgc agaaggtttt ggagaacgtg
      421 agtgtctcaa ttcctgatgc agcacgcctg tgggcttcca tgcgaatgtc ggtatctgcc
      481 tgatcatagg cctcatcacg cgaggaggtt atctcaaaga ctgggatgtc accgtcggtg
      541 tacggcagga gagtaccttt cagctcaggc ggtgttgcga taccgagatc acctgtctga
      601 caagcaatga cactccagag atactccaca tcggagctgt cctcatgata ggttctctgt
      661 gtatgtccat ccttgtcggt gtgggtggag tacccgcagg ccactccttt cccagtacct
      721 acaacgcgcc aaaacggaag atacatgggg aagatctcgg taatcaccgc agttgcggaa
      781 agatcacgtg ccttcgggaa agccccaaac catttacggg cagaacgctc cgcctcagcc
      841 tcggtgatct ccattttgta catgaggcgg gtaacaccgg catcatcggc agtgatggca
      901 agagccgagc cacagtaggg acaaaacgca agtctgcttc cctcggtcac ctcgattgta
      961 ccgccgcacc cggggcagga gagaccgata agaagttttg gcacactgct catttgctct
     1021 cacctccatc aggagtacgg accactgcac gccccgtcag aacagccaaa gccgcccccg
     1081 caaagacaag gacatagaag agcggtgttg taacaaatcc gaggattccg ccgataaagg
     1141 ccagaccaaa accgccgccg acaacaagcg catacggggc ggcggaccgt ccgggaaacc
     1201 cggatgtatg aacacgtccg gatgtccctc ccatgacgga gatgtactcg actccctgat
     1261 accggtagcg gaagacgaag aacggcagat aaagcagggc ctgttccttt gcagtgcctg
     1321 gaagatccgc gagatacgtc tcaacgctga tctccggatg aattacctca gcacccgcgg
     1381 tggagacggt agagtcaaac acctgaatat ctccaggcgg gattacctga ctctgaagac
     1441 cgggcagtgt tgttcctttg gcgggtttaa tgatggactt ctccggacca tcaacaacgc
     1501 ggcggaaaag aaagaccggg aagtatattt tttcaactga ggtaatctgt gcttcccgtt
     1561 caagatcctt tgcaagcgag gggcccgcac accaccgacg gaagacggca cgcgccttgt
     1621 cttcatcgag gatgaaaggc atgatgtagt gaaagatgac gctgcttcga tcaatgtaga
     1681 tgacggttcc gcagttctca cagatggtgt actgctttcc g
//
LOCUS       DALTZZ010000018         3816 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_3406,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000018 DALTZZ010000000
VERSION     DALTZZ010000018.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3406"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            18..314
                     /locus_tag="O0X12_00295"
     CDS             18..314
                     /locus_tag="O0X12_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HJK52633.1"
                     /translation="MAGNEINVQIPQNLDPVYSNMIQIAFKDDEFTMMFLHQLPAVNQ
                     AKAKAIVSISPSHAKKLLVALQKSVNEYEEKFGSIAPSAQPDAGSDGFTLRGYS"
     gene            401..472
                     /locus_tag="O0X12_00300"
     tRNA            401..472
                     /locus_tag="O0X12_00300"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:433..435,aa:Thr,seq:cgt)
     gene            complement(596..3028)
                     /locus_tag="O0X12_00305"
     CDS             complement(596..3028)
                     /locus_tag="O0X12_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobe/amphiphile efflux-3 (HAE3) family
                     transporter"
                     /protein_id="HJK52634.1"
                     /translation="MCIIYTGQCSILLVFFGCFSLEIPQIPFFVIRQVYVDSKKTYYQ
                     HFGEALPVISPLEKISDFIVQRPFLVGCFLIALIMLSLVGASTLSMSTETQDADKNAH
                     GAYISESYSNNFQSDSIMLMVQADSVMDVTVVDRVYTLQQQMATQQGVASVQSVYDVL
                     AAYNGGTIPQNQAIIDNIVAMLPEEITSSIMPNGQLAIILIKLNTGTSSDAQQSILDN
                     LRSMASSADIPPGVTLTFTGNAAMMQDMAGDLGSNMIILLVAAIVLMMIACKVLFNHV
                     RFPLLSIVSVFTGLILTFGMMGLFGLPFSMTTIGAMPILLGIGVDYAIQFHSRLDDER
                     QNNRPLPEAIKLAVSKTGAAVFYAMVASALGFMAMMVSTLPDIRQFGITAILGIVCCY
                     IAALIIIPLAAVITDYQPKPRKASADGGKKETFSEKYNELLRKLAVKVTKFAIPIMLI
                     LCIIGFTGVYLDEEVPVSTDMTSYVPVDMPAIVNINTVTRAMGDTGGFQVYVQGGDLT
                     SPDAIKWMYEWGEHELSLYNTRFTSVSSIATVIVEKNGGVLPTTQVQIDNILNSMSDE
                     DKASYIIDNSQAVISFGMKSLSEAQQRSLVSEVTADVQFFSPPPGIEAVVTGSAYSFV
                     EIMNDIREGKTKMTLLAFVIIFAFLALLYRSAGMAICPLVPIVLIIGWNGAAMYFFGI
                     DYTILTATMGAMTIGVAAEYCIMMVERIYEEMEHHDTLTAVQNGTGKIGTAITVSGCA
                     TMCGFSALMFSNFPIISGFGMVTVIAMGFTLFGAVVAVPATVSIVLKNRKKDRIEGDG
                     DCTHNSMNCPAL"
     gene            complement(3097..>3816)
                     /locus_tag="O0X12_00310"
     CDS             complement(3097..>3816)
                     /locus_tag="O0X12_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HJK52635.1"
                     /translation="VLRRIRRKHGVTDAADAIHMQPCVFDILVCDGETLIDRPFSERR
                     QILEKVMKDFVAPQLVSDSPDEIESYYHAALDNGNEGIMLKVLDSPYLPGNRGKLWVK
                     IKPEVDTIDLVVTGAEWGEGKRAKMFGSFLLACQDENGDLLELSRVATGIDDAMLAEL
                     YGLFKDKIIAEHGKTVVFEPDVVFEVGYAELQKSTTYAAGYALRFPRFVRLREDKDPS
                     EVETIESLFRRYSMQNKAGIE"
BASE COUNT         1100 a          960 c          811 g          945 t
ORIGIN      
        1 ctaatagtat tatacttatg gcagggaacg agattaatgt ccagattccc cagaatcttg
       61 acccggtgta cagtaatatg attcagattg ccttcaagga tgatgagttt acaatgatgt
      121 ttctgcatca gcttccggca gtgaatcagg caaaagccaa agcaattgta tccatcagtc
      181 cgtcacacgc aaaaaaactg ctggttgcac tccagaaaag tgtcaatgag tatgaagaaa
      241 agtttggatc tatcgcacct tcagcgcaac cagatgctgg atctgatgga ttcactctgc
      301 gcggatactc ataaccagac ctcgggactg cctttcctaa aggataggca gataacttat
      361 ttattgggtc acgtgcaatt tgataaagca cattttttgt gccgttgtgg cttagcggta
      421 tagcggctga ttcgtaatca gcaggtcgag ggttcaagtc cctccaacgg ctctctcatt
      481 ttggcaatac atccataaag aaactgtctt gaacatattt cgaacaaagt ggaatactgt
      541 atccaaggcc caaaataaac ggcttacaga actacccacg gagtaaaaaa ggagattata
      601 aggcggggca gttcattgag ttgtgagtac aatcaccgtc accctcaatt ctgtccttct
      661 tgcggttctt taacacaatc gacacagttg caggaacggc aaccaccgca ccaaacagtg
      721 taaagcccat ggcaattacc gtcaccatac caaatccgct gatgattggg aagttggaga
      781 acatcagtgc tgagaatccg cacattgttg cacaaccgga cacggtaatt gcggtaccaa
      841 tcttgccggt accgttttgt accgctgtta gtgtatcatg atgctccatc tcctcataaa
      901 tacgttccac catcatgata caatactcag cagccacacc gatggtcatc gcccccatgg
      961 ttgctgtcaa gatcgtataa tcaataccaa agaagtacat cgccgcaccg ttccatccaa
     1021 tgatcagcac gatcggtacc agtggacaga tagccatacc tgcactgcga tagagaagtg
     1081 caaggaatgc aaagatgatt acaaacgcaa gcaatgtcat cttcgttttt ccttcgcgga
     1141 tatcattcat gatctcaaca aaggagtacg cactgccggt aaccacagcc tcaatacctg
     1201 gcggtggtga aaagaactgc acatccgcag taacttctga gacgagcgac cgctgctggg
     1261 cctccgacaa ggatttcatg ccaaagctga ttactgcctg ggagttgtcg ataatatagg
     1321 acgctttgtc ctcatcactc atggaattca gaatattatc aatctgaacc tgggtagttg
     1381 ggagaacacc accattcttt tccacgatta cggttgcaat actcgaaacg ctggtaaacc
     1441 gtgtattgta caacgatagc tcatgctcac cccattcata catccacttt atcgcatcag
     1501 gactggtcag atctcctccc tgcacataga cctggaaacc tccggtatca cccattgcac
     1561 gtgttactgt attgatattc acaatggcag gcatatctac cggcacatac gatgtcatat
     1621 cagtactaac aggaacttct tcatccagat acacccctgt aaatccaatg atacagagaa
     1681 taagcatgat agggatggca aactttgtca ccttcacggc aagttttctt aaaagctcgt
     1741 tgtacttttc ggagaatgtt tctttttttc caccatctgc cgaagctttt cgtggttttg
     1801 gctgataatc tgttataaca gctgccagcg gaatgataat cagcgcagca atgtaacaac
     1861 agacaatacc aaggattgct gtaataccaa actggcgaat atccggcaga gttgagacca
     1921 tcattgccat gaatccaagg gcactggcaa ccattgcgta aaacaccgca gctcccgtct
     1981 tggatacggc aagtttgatt gcttcaggaa gggggcgatt gttttgcctc tcgtcatcca
     2041 aacgggagtg gaactgaatt gcatagtcca caccaatacc caggagaatt ggcattgcac
     2101 caatcgttgt cattgagaac ggcaggccaa agagtcccat cataccaaac gtcagaatca
     2161 gaccggtgaa cacactcaca atcgacaaca gagggaaacg tacgtggttg aacaggactt
     2221 tacaggcaat catcatcaaa acaattgctg caaccagcaa aatgatcatg ttcgagccaa
     2281 gatcaccggc catatcctgc atcatagctg catttccggt aaaggtcagc gtgacaccag
     2341 gaggaatatc agcagatgat gccatgctcc ttaagttatc gagaatactc tgctgcgcat
     2401 cagaggacgt tcccgtgttc agtttgatca aaataatcgc aagctgacca ttcggcatga
     2461 tagaggaggt aatctcttcg ggcagcattg caacgatgtt atcgatgatt gcctgattct
     2521 gcgggatcgt tccgccattg taggcggcca aaacatcata cacactttgt accgacgcaa
     2581 ctccctgctg tgttgccatt tgttgttgta gtgtgtaaac acgatccaca acagttacgt
     2641 ccattaccga gtctgcctgc accatcagca tgatggagtc agactggaag ttattcgaat
     2701 aactttccga gatgtacgcg ccatgagcgt ttttgtccgc atcctgcgtc tcggtactca
     2761 tagaaagagt acttgctcct acaagtgaga gcataatgag agcaatcagg aaacagccta
     2821 ccaaaaacgg ccgttgtaca atgaagtcac tgatcttttc aagtggtgat atcacaggaa
     2881 gtgcctcgcc aaaatgctgg taatatgtct tctttgagtc tacataaact tgccttataa
     2941 caaaaaaggg gatctgcggg atttccaacg aaaaacagcc gaaaaagaca agaagaatac
     3001 tgcattgtcc cgtgtaaata atacacatcc tgtgtctgat gccctgatca acagacaagc
     3061 agagaagtta ggcaggatct gctgtcagat ccaagattat tcaattcctg ccttgttctg
     3121 catgctgtat ctgcgaaaca gagattcaat cgtctcaacc tcactgggat ctttatcttc
     3181 acgcaggcgg acgaaacgcg ggaaccgcag ggcatacccc gcagcatagg tagtactttt
     3241 ctgcagctcg gcatacccga cttcaaagac aacatccggt tcaaacacca cggtcttacc
     3301 atgctcagcg atgattttgt ctttaaacaa cccatacaac tctgcaagca tagcatcgtc
     3361 aataccggtt gccacccgac tcaactccag cagatcacca ttttcatcct ggcaggcaag
     3421 cagaaacgaa ccaaacatct ttgctcgttt accttcaccc cattcagcac cggtaacaac
     3481 aagatcaata gtatcgacct ccggcttgat cttgacccag agtttcccac ggttcccggg
     3541 aaggtaagga gaatccagaa ctttcagcat gatgccctca ttcccgttat ctagtgccgc
     3601 atgataataa gactcgatct catctggaga atcactcacc agctgggggg ctacaaagtc
     3661 cttcatcact ttttcaagaa tctgtctacg ctcagagaac gggcgatcga tcagtgtttc
     3721 accatcacaa acgaggatgt caaagacaca cggctgcata tgaatggcat ccgccgcatc
     3781 agtaacacca tgtttcctgc gtattcttcg cagcac
//
LOCUS       DALTZZ010000019         1705 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_3451,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000019 DALTZZ010000000
VERSION     DALTZZ010000019.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3451"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="O0X12_00315"
     CDS             complement(<1..201)
                     /locus_tag="O0X12_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HJK52636.1"
                     /translation="MFSKILVAIDGSEISRRAFSRAIDLAKQSKAELHVVYVIESGIV
                     SPAPVDTTWELIYERFEKEGHQV"
     gene            complement(285..701)
                     /locus_tag="O0X12_00320"
     CDS             complement(285..701)
                     /locus_tag="O0X12_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HJK52637.1"
                     /translation="MFSRILVALDGSEISHTAYARALEFAKADKAELHVVTVVNTPHL
                     WKDDSPANQTQASTDANLLINELESDAKEAGISFIPHIVTGHPGDRIVSTADEIDADL
                     IVVGSLGKSNLDRLLLGSVSAYVTQYSQRNILVIRG"
     gene            complement(752..>1705)
                     /locus_tag="O0X12_00325"
     CDS             complement(752..>1705)
                     /locus_tag="O0X12_00325"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HJK52638.1"
                     /translation="IEKGIITEISDSTQPINQWIAPAFFNAHTHIADTVAMDTPVGDH
                     SLAELVAPPDGLKHRILRATSDDCLCNAMRETMQFMYATGTSAFAEFREGGVHGVQLL
                     QQAFTSEIRPVIFGRDGGEFVADGLGLSSAKHPAEDLAAVEHARAAGKRIAIHAGEAG
                     TKDIDAAFALDPDFIIHATYFEDRHIREAADRGIPLVICPRSNWILGGTNSAARPQVR
                     KMIEAGCTLWLGTDNVMFVPPDMFAECAFLTTVYKLSPEEILSMATGGFSLLGRKGII
                     EEGEPANLICLDPGYVKEWTQSPALSLVSRIGSRSVRSVYS"
BASE COUNT          441 a          434 c          404 g          426 t
ORIGIN      
        1 aacctggtgt ccctcctttt caaaccgctc gtagatcagt tcccaggtgg tatccaccgg
       61 tgcaggactg acaattcccg actcaatcac atagactacg tggagttcgg ctttactctg
      121 ttttgccaga tcgattgccc gcgagaatgc acgacggctg atctcggaac catcaattgc
      181 taccagtatt ttggaaaaca tgactgtata cctccgatta tctcgttatg attatttacc
      241 tctccaaaac ttaaaaagag ttcggagcag gcagggcatg cgtttcaccc gcgaataacc
      301 agaatattcc gctgactgta ctgggttaca taggcagaaa cactgccgag aagcaggcgg
      361 tccaggttgg actttccaag ggagccgaca acgatcagat cagcatcaat ctcatcagca
      421 gtacttacga tccgatctcc cggatgacca gtaacaatat gcggaataaa ggagataccg
      481 gcctcttttg catcactctc aagttcgttg atcagcagat ttgcatctgt actggcctga
      541 gtctgatttg cgggactgtc atctttccac aggtgagggg tgttaacaac cgttaccaca
      601 tgtaactcgg ctttgtctgc tttagcaaac tctagagctc gtgcatatgc tgtatgactg
      661 atctcagatc catccaaggc cacaagaatt ctggaaaaca tgataatttt actgtcgtgt
      721 tataaggata aaaaatatcg gatgagtctg ttcagctgta cactgaacga acagaacgac
      781 tgccgattcg tgaaacaagg gaaagagctg gagactgggt ccactccttt acgtatccgg
      841 gatcaagaca gatgagattt gcgggttccc cctcctcgat gatgccttta cgtccaagaa
      901 gagagaatcc gccggttgcc atggagagaa tttcttccgg tgagagtttg tacacagtgg
      961 tgaggaatgc gcattccgca aacatatcag gagggacaaa catgacattg tcagtaccaa
     1021 gccagagagt acatcccgct tcgatcattt ttcgtacctg aggacgggca gcgctatttg
     1081 ttcccccaag tatccagttg gaacgcgggc agataaccag aggtatgcca cgatcagcag
     1141 cctcgcgtat gtgccgatct tcaaagtatg ttgcgtgaat gataaaatca ggatccaagg
     1201 caaaagctgc atcgatgtct ttcgtccccg cttcccccgc atgaatagca atccgttttc
     1261 ctgcggcacg tgcatgttcc actgcggcga gatcttctgc tggatgtttg gcactgctga
     1321 gcccaagtcc atccgcaaca aactcaccac catctctccc gaagattacg ggacggattt
     1381 cagaagtgaa cgcctgttga agaagctgaa ctccatgtac gccgccttcc cggaactcgg
     1441 caaatgcgga tgttccggtc gcatacataa actgcatagt ctccctcata gcattacaca
     1501 ggcaatcatc cgaggttgca cggagaatcc ggtgtttcag tccgtccgga ggtgcaacaa
     1561 gctctgcaag tgagtgatca cccaccggcg tatccattgc aacggtgtca gcaatgtgcg
     1621 tatgtgcgtt gaaaaatgcc ggagctatcc actggtttat cggctgcgta ctgtccgaaa
     1681 tttcagtaat tattcccttt tcaat
//
LOCUS       DALTZZ010000020         3711 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_3472,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000020 DALTZZ010000000
VERSION     DALTZZ010000020.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3472"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="O0X12_00330"
     CDS             <1..411
                     /locus_tag="O0X12_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HJK52639.1"
                     /translation="VQIFSLILIYIAIYLPPAEYMLEFQSSFAETLGTSARFVLASLV
                     AYGVAQTLDVHLFHWLRGRFEPKWMRNNGSTMVSQLVDTVIFITIGFWGVVPNLGWMM
                     VSQYVVKWVFALADTPVFYYLTRDHGKQAVKTKE"
     gene            complement(682..3222)
                     /locus_tag="O0X12_00335"
     CDS             complement(682..3222)
                     /locus_tag="O0X12_00335"
                     /inference="COORDINATES: protein motif:HMM:NF041431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMAR_RS02690 family S-layer glycoprotein"
                     /protein_id="HJK52640.1"
                     /translation="MAVLVVLLAAMLFVGAASAADNIYRNVSSNGGLAFVYETINISV
                     NDDMGSEKPVLVKFSEGSSPSAVNTIYGEWKEEGSSAIFTLYDTAVGSNYGTYYATAN
                     YSNVSKVGSVSIWYPELTLKAELATPGSLVGTVYQATTAGDSIDGKSITKNTNVTFLI
                     DAPRVGPAYNATNFTAAAQIMFTTPAAGKTTYFGDFNFDKTNLTATQVQANATKATDA
                     GKIYKYASAGSDAAAGTYTAYAEFKAFKVDGGKEFTGFKDNAKKSNTITFTVQSTTLT
                     LAAGKDSVIRSTPFTVDIQGDSNALYFVYLDGASVSDVNPTLQPGQAGMKSTVNSENV
                     PKYDKSPVIDEKDATKLVGTYGFFETDASGKRTIQYNTNASTEDKSYTVKVYAIKEDW
                     RGEDATYNATSDYDTVKIKVEKGAVTITAGGDGAYYIGQEITLSGTNSDSDVVYLFIT
                     GPNLASDGVILKKVGERLSDEKGAEARYAAESDTVSVKTDDSWEYKWDTSNCNLNTGS
                     YTIYATSVLTNGRSSSPSGDAVKLSDSEYATVSVSLKQPFLSAVPSGTVVAKGDDIYI
                     RGTAEGEPSKLQLYIFGPNKFVYESITVEDDGTYEKKLNIGSDWASSQYYVVIEHPMY
                     NGEIDVKYDEKAGEIFIKNVVGDNAQSSFNVNKLQSSNAADALTKMIDNPNIDDVYTK
                     LTFTVVPAMITINNPGDQGVGTKFTISGTTNLAVGDQIIVEVQSSSFTAVDKSSTSST
                     DGVSVTTKVVAGEGTDNVWSVEVDTTNWKLDEYTIRAEGIEVDVSATTNFNLVEKTVT
                     PTATATATGTATATATATATATPATPGFGAFVALAGLGAVALLVLRRN"
     gene            3393..>3711
                     /locus_tag="O0X12_00340"
     CDS             3393..>3711
                     /locus_tag="O0X12_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52641.1"
                     /translation="MVKTDQNLHFRTTSIQKSQKNPNSARKQHPISSTIREKTPKPTS
                     TNNGQNPHTPSCRTSTTQLAQRTIIIVHHPTPKPKERSKDISSHTHYTITNQQKTICG
                     QSGN"
BASE COUNT          907 a          844 c          922 g         1038 t
ORIGIN      
        1 gtacagatct tttcgctgat cctgatctat attgcaattt atctgccgcc ggcggagtac
       61 atgcttgagt tccagagttc gtttgcggag acgcttggta cgagcgcccg gtttgttctt
      121 gcgtctctgg ttgcctatgg tgttgcgcag accctggatg tacatctgtt ccactggctg
      181 cgggggcggt ttgagccgaa gtggatgcgg aacaacggaa gtacgatggt gagtcagctg
      241 gttgatacgg tgatctttat taccatcggg ttctgggggg ttgtgccgaa tctgggatgg
      301 atgatggtga gtcagtatgt agtgaagtgg gtgtttgctc ttgcagatac accggtgttc
      361 tattatctga cgcgggatca tggaaagcag gctgtaaaga caaaagagta atttttgccc
      421 agctattttt tctactcatc attttggagg gagaactggt atctcctatc ttctgttttt
      481 gatggattgg ctctgggcag gactattgtc ttgggcaact gtacggagaa taatttgtat
      541 cacacaccga ttgttttcga atcataacat cggtgtttga gcatttgtaa aaaacagctt
      601 cagactcttt tcaaaaaaga gattggtatc tctcaggagt tttcctgagg atgtttcctc
      661 tctttttgtg gttcacgctg cttagttgcg gcggaggacg agcagtgcta ctgcaccaag
      721 tcctgcgagt gcaacgaatg cgccgaagcc cggagttgcc ggggttgcgg ttgcagttgc
      781 ggttgcagtt gcagttgcgg ttccggttgc agttgcggtt gcggtcgggg ttacagtctt
      841 ctcaacaagg ttgaagttgg tcgtagctga tacatcaacc tcaatacctt cggctctgat
      901 ggtgtactca tcaagcttcc agttggtcgt gtcaacctca acagaccaga cgttgtcagt
      961 accctcgcct gcaacaacct tggtggttac agagacacca tcggtggatg aggtggagga
     1021 cttatcaact gcagtgaagg atgaggactg tacttcaacg atgatctgat cgccaacagc
     1081 gaggttggta gtaccggaga tagtgaactt ggtgccaaca ccctggtctc ctgggttgtt
     1141 gattgtgatc attgccggaa caacggtgaa ggttagcttg gtgtaaacat catcgatgtt
     1201 cgggttgtcg atcatcttcg tcagcgcatc agctgcattc gaactctgaa gtttgttgac
     1261 attgaaggag gactgagcgt tgtcgccgac tacgttcttg atgaaaatct ctccagcctt
     1321 ctcatcatac tttacgtcaa tttcaccatt gtacatcggg tgttcaatga cgacatagta
     1381 ctggctggat gcccagtcag atccgatgtt cagtttcttt tcatatgttc catcgtcctc
     1441 aacggtgata gactcataca caaacttgtt cggaccgaag atgtagagct ggagtttgct
     1501 cggttcaccc tctgcggttc cgcggatgta gatgtcatcg ccctttgcga caacggttcc
     1561 ggacggaact gcggagagga acggctgttt cagagagacg gaaacagttg catactcaga
     1621 gtcggagagt ttgacagcat caccagacgg agatgatgat ctgccgttgg tcagcacact
     1681 ggttgcgtag atggtgtacg atccggtgtt cagattacag ttggatgtat cccacttgta
     1741 ctcccaagag tcatcggtct ttacagaaac tgtatcggac tctgctgcat atctggcttc
     1801 tgcacctttc tcatcagaga gtctttcacc tactttcttc agaataacac catcagaagc
     1861 aaggttcgga ccagtgatga agaggtaaac aacgtcactg tcggagttag ttccggagag
     1921 cgtgatctcc tgaccaatgt aataggcacc gtcgccacct gcggtgatgg ttacagctcc
     1981 cttctcgact ttgatcttga cggtgtcata gtcggaggtt gcattgtaag tagcatcttc
     2041 accgcgccag tcctctttaa ttgcatagac ttttacggta taactcttat cttcggtaga
     2101 tgcattggtg ttgtactgga tggtacgttt accggaagca tcggtttcga agaatccata
     2161 agtgccaact aacttcgtag cgtccttttc gtcgatgaca ggactcttgt cgtactttgg
     2221 aacattctct gagtttacag tactcttcat tcctgcttgt cctggctgaa gtgtcgggtt
     2281 gacatcagat acggatgctc cgtcaagata gacgaagtac agtgcattgc tgtcaccctg
     2341 gatatcaaca gtgaacgggg tgctacggat aacagagtcc tttccagcag caagggttaa
     2401 cgttgtactc tgcacggtga aagtgatcgt gttggacttc tttgcattgt ccttgaatcc
     2461 agtaaactct ttgccaccat cgactttgaa tgccttgaac tcggcatatg cagtgtaggt
     2521 acctgctgct gcatcagaac cggcagaggc atacttgtag atcttacctg catcggttgc
     2581 cttggttgca tttgcctgaa cctgcgttgc agtcagattg gtcttgtcga agttgaagtc
     2641 accgaagtag gtggttttac ctgcggcagg ggtcgtaaac atgatctgag ctgctgcagt
     2701 gaagttcgtt gcattatatg caggaccaac tctcggagca tcaatcagga atgtaacgtt
     2761 agtgttcttg gtgatacttt ttccgtcaat ggagtcacca gcagtcgttg cctgatacac
     2821 ggtaccgaca aggcttccgg gggttgcaag ctctgccttc agtgtaagtt cggggtacca
     2881 gatggaaact gatcctacct ttgagacatt agagtagttg gcagttgcat agtatgtacc
     2941 gtagttggaa ccaactgcag tatcatagag cgtgaagatt gcagaggatc cctcctcttt
     3001 ccactcacca taaatagtgt ttactgccga tgggctgctt ccctctgaga acttaaccag
     3061 aacaggcttc tcagatccca tgtcatcgtt aacagagatg ttaatcgtct catagacgaa
     3121 ggcaagtccg ccattgctgc tgacgtttct gtaaatattg tcagctgcag atgctgctcc
     3181 tacaaagagc attgctgcga gcagaacgac gagaactgcc attattttcg tgtttttcaa
     3241 gtgtaattcc tcctaaatta ggatttaatt tttctgcatg tgatacatgc aatacgtgac
     3301 attcgacaga tgtcgaataa tcattctgca agggttatac tccgatcaac ggaatatgca
     3361 ttgatatttg tatatggggt ataataggct ttgtggtcaa aacagatcaa aacctgcatt
     3421 tcagaacaac atcaattcaa aaatcgcaga aaaatccgaa cagcgcaaga aaacagcacc
     3481 caatctcatc gacaatcagg gaaaagaccc caaaaccaac ttcgacaaac aacggacaaa
     3541 accctcacac cccctcctgc cgcaccagca ccacacaact cgcgcagcgc actattataa
     3601 tagtgcacca tcccactcca aaacctaaag aacgctcaaa agatatatcg tcacacacgc
     3661 attatactat cactaaccaa cagaaaacaa tctgtggcca aagtgggaac a
//
LOCUS       DALTZZ010000021         1967 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_3475,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000021 DALTZZ010000000
VERSION     DALTZZ010000021.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3475"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="O0X12_00345"
     CDS             <1..528
                     /locus_tag="O0X12_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52642.1"
                     /translation="AQRIAAEDAERKRLEKEAKSKSRKRKIIGGVILLIVVVLIVLVA
                     SSGISVQPGTATNYPYITTYDVWFPIGEQVDISGHKLIALSDGNEMMFSTDGSVTKLV
                     RNEPITIGEQSVTLTTLFGSVPVMTVNYKVILEYQGNTEANQAYFKMLIQSDKSIPEV
                     FVNLLLPNNVIARPA"
     gene            540..1508
                     /locus_tag="O0X12_00350"
     CDS             540..1508
                     /locus_tag="O0X12_00350"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HJK52643.1"
                     /translation="MTTKASVLRILEENRDRFFSGEEIASMLGVSRSAVWKAVKSLEA
                     DGYAVSAVTNKGYCLSADTDILSPEGIRLYLSPKYRDLPITIHKVTGSTNDDAKTLAV
                     AGAVHGTMVLAEEQTAGRGRLGRSFYSPKRSGVYISIVLRPNLAITDAVLITTAASIA
                     VCRAVEELSGEKPEIKWVNDVFVQGKKICGILTEAVSGFESGAVECVVVGIGINVVDT
                     GFPDDIKQVAGSLFSEHQEFSRNHLAALVANHLLDLSSALPDRSFLDEYRTRSLLLGK
                     PIRFLENNVWHDAVAVDVDNSGGLVVDTDTGRRTLSSGEVTVRPIR"
     gene            complement(1501..>1967)
                     /locus_tag="O0X12_00355"
     CDS             complement(1501..>1967)
                     /locus_tag="O0X12_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HJK52644.1"
                     /translation="VAPIFWFAVFELLFGMGIVGAALFRAANTMDAMLGYTDDRIRIG
                     WFSARLDDVLAWIPARVTGALLILIFVCRKRAGKAVAAYRADHKKRPGFNGGIPMSLI
                     AGGCGVMFDKPGVYTIGIPEQTLEKGGRCIIRTLRETLILFSAVVILTLVLI"
BASE COUNT          483 a          501 c          498 g          485 t
ORIGIN      
        1 gcacagcgga ttgcggcgga ggatgccgag cgcaagagac tggaaaagga ggcaaaaagt
       61 aaaagtcgca aaagaaaaat cattggcggt gtcatcctcc tgatcgttgt tgtgttgatc
      121 gttctggtag cttcatcagg aatttctgtc caaccaggta ccgcaaccaa ctatccttac
      181 atcactactt atgacgtctg gttcccgatt ggtgagcagg ttgacatcag cggccacaag
      241 ctgatcgctc tttccgatgg caatgagatg atgttctcca ctgacggtag tgtgaccaaa
      301 ctcgtccgta atgagccgat aacaataggc gagcagtctg taacgctaac caccctcttt
      361 ggcagcgttc cggtaatgac ggtaaactac aaagtcattc ttgagtatca gggaaacact
      421 gaagcaaatc aggcctactt caagatgctt atccagagtg acaaatccat tccggaggtg
      481 tttgtcaacc ttctgctgcc gaataacgtc attgcccgac cggcataacc ccctttttta
      541 tgacaaccaa agcgtctgta cttcgtatct tagaggaaaa ccgcgaccgg ttcttttccg
      601 gggaagagat agcatccatg cttggagttt cccgttctgc ggtgtggaaa gcggtgaaat
      661 cacttgaggc agacggttat gcggtgtccg cagtgacgaa caagggctac tgtctttctg
      721 cggatacaga tattctgtca ccggagggta tccggctgta tctttcacca aaatatcggg
      781 atcttccgat tactattcac aaagtaaccg gttccacaaa tgatgatgcg aagactcttg
      841 ctgttgcagg ggctgtacac ggaacaatgg ttcttgcaga ggagcagact gccggtcgcg
      901 ggagacttgg ccgatccttt tactcgccaa agcgttccgg agtttatatc agcattgtcc
      961 tccggccaaa tcttgcgata acggatgccg ttttaatcac aaccgcggct tccattgccg
     1021 tatgccgtgc agttgaagag ctgtccggtg aaaaacctga gatcaaatgg gtcaacgacg
     1081 tgtttgtgca gggtaaaaag atctgcggca ttctcactga ggcggtcagc ggctttgagt
     1141 ccggtgcggt tgagtgtgtt gttgtcggta ttggcatcaa tgtggtggac acaggttttc
     1201 ccgatgatat caaacaggtt gcaggttcgc tcttcagtga acatcaggaa ttttcccgca
     1261 atcatcttgc agcgcttgtt gcaaatcatc ttcttgatct ctcctctgca ctgccggatc
     1321 gatccttcct tgatgagtac cgcacacgat ccctgcttct gggaaaaccg atccgttttc
     1381 tggaaaacaa tgtctggcat gatgcggtcg ccgtggatgt ggacaactca gggggccttg
     1441 tggtggatac cgacaccgga cgcaggaccc tttcctccgg cgaagttacc gtccgcccga
     1501 tcagataagt acaagcgtca gaattaccac tgcggagaac agtattagtg tttcccgcaa
     1561 tgttctgata atacaacgcc ctcctttttc caatgtttgt tcgggaatac cgattgtata
     1621 cactccgggc ttgtcaaaca tcacaccgca tccaccggcg atcagactca ttggaatccc
     1681 gccgttgaag ccgggacgtt ttttatggtc tgcacggtat gctgcaaccg cctttcctgc
     1741 acgtttacgg cagacaaaga taaggatcag cagggcgccg gtgactcgtg ccggaatcca
     1801 ggcaagaaca tcatccaacc gtgcggaaaa ccagccgatg cggatccgat cgtctgtgta
     1861 gccgagcatc gcatccatgg tatttgccgc ccggaacagt gcagccccaa caattcccat
     1921 tccgaacagc agttcaaaga cagcaaacca gaatatcggt gcaacaa
//
LOCUS       DALTZZ010000022         1884 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_3706,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000022 DALTZZ010000000
VERSION     DALTZZ010000022.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3706"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(39..440)
                     /locus_tag="O0X12_00360"
     CDS             complement(39..440)
                     /locus_tag="O0X12_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJK52645.1"
                     /translation="MITLEHGGTSENAEHQDICFCPLHGLLDTISKKWALMIIAVIGN
                     HGSAGFNELKRQLYNVSSKTLSNTLKDLEEHGLVYRQVVDQTPPVVRYYLTVSGWELR
                     ELLIPLLAWVMKNGGHADEGCPIHFNAKNVS"
     gene            573..965
                     /locus_tag="O0X12_00365"
     CDS             573..965
                     /locus_tag="O0X12_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52646.1"
                     /translation="MNKTEIIAVTFAVLVVFCGIGAGIGGLIVEEANGNYVQGLADKT
                     GTEIVKLTANDGSGAVAFRTADGIYIDLLSMINAYNGLSEIVVVYNVNNGQITGEQNA
                     AISFNHAAGLLSSPAGALLFNLAEAGQN"
     gene            972..>1884
                     /locus_tag="O0X12_00370"
     CDS             972..>1884
                     /locus_tag="O0X12_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HJK52647.1"
                     /translation="MPKQIKDPVHGYIEVPAEILPLVDSPALQRLHHIRQLGFAYLVY
                     PGANHTRFEHSLGAMHLASLLCRHLGFGTAETRTICCAALLHDIGHGPYSHASERLMQ
                     EYTQFSHDDIREQLKEPILAKQLEINSISPDEVAALISGSHRYAGIIHGDLDVDRMDY
                     LLRDAHYTGAPYGNFDAERLTKSLAVVDNTIVLNESGISAAESLLIARTLMGPSVYYH
                     HACRIAEEMFLLACRSHFRKDVDAVREFLTMDDVTATALLLNSDEEITRDLINRIRTR
                     NLYKRAVYAGKELVDRDHLPQTKSGIER"
BASE COUNT          515 a          501 c          435 g          433 t
ORIGIN      
        1 gtcgcagaca aataccctct gccggagtac aaaaccactt aggatacgtt ctttgcgttg
       61 aagtgaatcg ggcacccctc atctgcatga ccgccgtttt tcatcaccca tgcaagcaat
      121 ggaatcagaa gttcacgcag ctcccatcca gagacagtga gatagtaccg gactaccgga
      181 ggggtctgat caaccacctg acggtaaacg agaccatgtt cctcaagatc cttcagcgta
      241 tttgacagtg tttttgaact cacattatag agctgacgtt tcagctcgtt gaatcctgcc
      301 gagccgtgat tgccgataac cgcaatgatc atcagcgccc actttttgct tatggtatcc
      361 aacagaccgt gcagaggaca aaaacagatg tcctgatgct ccgcattctc agaagtaccc
      421 ccatgctcca aagtgatcac ctcaatatac tcatacgtac tgatagagga taaattctga
      481 cactaagtta cgaaaggaaa ccatcttgca tcccgaaaag tacagtgtaa agattattta
      541 acaaatacac gcatatagtt atcattatgg gaatgaacaa aacagaaatc attgcggtaa
      601 cattcgcagt actcgtcgtc ttctgtggca ttggtgccgg aatcggcggt ctcatcgttg
      661 aagaagcaaa tggaaactat gttcagggac tggcagataa aaccggaaca gagatcgtaa
      721 aactgactgc caatgacggt tcaggtgctg ttgcattccg taccgcagac ggtatctaca
      781 tcgatctcct ctctatgata aatgcctaca acggactctc cgagatcgtc gtggtgtaca
      841 acgtcaacaa cggacagatc accggtgaac aaaacgcagc aatttccttc aaccatgccg
      901 caggacttct cagctcaccc gcaggagctc tgctgtttaa ccttgcagag gcaggacaga
      961 actagatcct catgccaaag cagatcaaag acccggtaca cggatatata gaagtccccg
     1021 cagaaattct gccgctggtg gactcaccgg cactgcagcg gcttcaccac atccgtcagc
     1081 ttggctttgc atatctggtg tatcccggag caaaccatac aaggtttgaa cattctcttg
     1141 gtgcgatgca cctcgcatct cttttatgca gacatctcgg gttcggaact gcagagacac
     1201 ggacgatctg ttgtgctgca cttctccacg acattggtca cggaccgtat tcacatgcaa
     1261 gcgaacgact gatgcaggag tatacccagt tttcgcatga tgatattcgc gagcagctga
     1321 aagaacccat ccttgcaaaa caacttgaga taaattccat aagccctgat gaagtcgcag
     1381 cgctcatctc cggcagccac agatatgccg gcatcatcca tggtgacctg gatgtggacc
     1441 gcatggacta tctcctgcgg gatgcccact acaccggtgc accgtacgga aacttcgatg
     1501 cagaacggct gacgaagtct cttgcagttg ttgacaacac gatcgttctc aacgagtcag
     1561 gaatctcggc tgctgagtca cttctgattg cacgaacact aatgggcccg tccgtctatt
     1621 accatcacgc ctgccggatt gcagaggaga tgtttcttct tgcatgccgc agtcatttca
     1681 ggaaagatgt agacgcggtc cgcgagtttc tgacaatgga cgatgtgacc gcaacagctc
     1741 ttctgctaaa ctcggatgag gagataaccc gcgatctcat caaccgcatc cgcacacgaa
     1801 atctctacaa acgggcagtg tacgctggga aggaactggt tgaccgggat catctccctc
     1861 agaccaagag cggaattgaa cgac
//
LOCUS       DALTZZ010000023         2357 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_3794,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000023 DALTZZ010000000
VERSION     DALTZZ010000023.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3794"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="O0X12_00375"
     CDS             complement(<1..261)
                     /locus_tag="O0X12_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase"
                     /protein_id="HJK52648.1"
                     /translation="MKQQSPSKETLCRELYTKTGYVFAKSPAEIAAASGFAEVAMLGS
                     NENPYPPSPAVLKAAEAALATANRYPDPKTEEFIAALRRYVYD"
     gene            complement(239..1366)
                     /locus_tag="O0X12_00380"
     CDS             complement(239..1366)
                     /locus_tag="O0X12_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinylornithine family
                     transaminase"
                     /protein_id="HJK52649.1"
                     /translation="MPTSKELDEKYYMPAFGRDMEIVLGKDCHVWDSTGKKYLDLVAG
                     IAVCSTGHCHPAVTTAICKQAQTLIHCSNLYYVPGQAELAEKLSKASGLGKVFFGNSG
                     AEANDAAMKLAIRATGRHEFVSFEHDFHGRTVGSLACTHKPAIREPFEPLGIPCKFSP
                     YGDIEALKEFVTKKTAAVILEPIQGETGVIIPPDDFLPGVRDICDDAGALMICDEVQT
                     GMGRTGKWFAFQHSSIQPDIISLAKGVASGIPMGAIIAREGLEFTRGEHGSTFAGGPI
                     ACAAGNATFDVLKPLVPKVTAKGERFRKGLEKLNPRVRGLMIGFTAGEKAPAIAEYCR
                     EHGVLINVAGGENIRIVPPLVIENREIDYAIGIINEAAESV"
     gene            complement(1372..2316)
                     /gene="guaA"
                     /locus_tag="O0X12_00385"
     CDS             complement(1372..2316)
                     /gene="guaA"
                     /locus_tag="O0X12_00385"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HJK52650.1"
                     /translation="MSSFVDNMVAHLREKIGDKKVLCALSGGVDSSVAAVLVHKAVGK
                     QLTCIFVDHGLLRKNEGDEVEEVFRNQYDINLIRVNAEDRFLAKLAGVSDPEQKRKII
                     GAEFIHVFEEEAKKIGTVDFLVQGTIYPDVIESGPGNAAVIKSHHNVGGLPDHVDFKE
                     IIEPLRILFKDEVRAAGVELGIPEHLVWRQPFPGPGLAIRVIGDITKEKLDILRDADA
                     IFREEVAAAGLGRAINQYFAALTNMRSVGVMGDERTYDYAIALRAVTTTDFMTADWAQ
                     IPYPVLEKISSRIINEVRHVNRVLYDITSKPPATIEYE"
BASE COUNT          547 a          665 c          601 g          544 t
ORIGIN      
        1 atcatagaca taccggcgca gtgcagcgat aaactcctct gttttaggat caggataccg
       61 gttcgccgta gcaagcgcag cttccgctgc cttgagaacc gccggagacg gaggataggg
      121 attttcatta ctaccaagca tggcgacctc ggcaaatccc gacgccgccg cgatctcagc
      181 cggggacttt gcaaagacat aaccggtctt tgtgtaaagt tcacggcaga gagtctcttt
      241 agacggactc tgctgcttca ttgatgatgc caatagcgta atcgatctcg cggttctcaa
      301 ttacgagcgg tgggacaatc cggatgtttt ctccgccggc aacgttgatc agaacaccat
      361 gctcacggca gtactctgca attgccggag ctttctcacc agcggtaaat ccgatcatca
      421 gaccccgcac acgcgggttc agtttttcca gaccttttct gaaccgctcg ccctttgcag
      481 taacctttgg cacaagcggc ttcagcacat caaacgtcgc atttccggcg gcacaggcaa
      541 tcggacctcc tgcaaaggta ctgccgtgct cgcctctcgt aaactccaga ccctcgcgtg
      601 cgatgattgc acccatcgga ataccggatg caacaccctt ggcaagactg ataatatccg
      661 gctggatgga tgagtgctgg aaagcaaacc atttaccggt tctgcccata ccggtctgta
      721 cctcatcaca gatcatcaaa gctcctgcat catcacagat gtcgcggacg ccaggcagga
      781 aatcatcggg cggaataatg acaccggtct caccctggat cggttcaagg ataaccgcgg
      841 cggtcttctt tgtcacaaat tctttcagtg cctcaatatc cccgtaggga ctgaacttgc
      901 agggaatacc gagcggctca aacggctcgc ggattgcagg cttgtgggta caggcaagag
      961 aacccacggt tcttccgtgg aagtcatgct caaaggagac gaactcgtgg cggccggttg
     1021 cacggatggc aagtttcatt gccgcatcat ttgcctcggc accggagttt ccgaagaaga
     1081 cctttccaag accggaagcc ttcgagagtt tttcggcaag ctctgcctga cccggcacat
     1141 agtagagatt agagcagtgg atcagcgtct gcgcctgttt gcagatagca gtggttaccg
     1201 ccggatgaca gtgcccggta ctgcacaccg caatacctgc gaccagatca agatattttt
     1261 tcccggtact gtcccagacg tggcagtctt tccccaggac gatctccatg tcccgtccga
     1321 aggcgggcat atagtatttt tcatcaagtt ctttggaggt tggcatacgt atcactcata
     1381 ttcgatggtt gcagggggtt tgctcgtgat atcatacaga acacgattca cgtggcgaac
     1441 ctcattgatg attctgctgg atattttttc cagtaccgga tagggaatct gtgcccagtc
     1501 cgcggtcatg aagtcggtcg tcgtcaccgc acgcagtgca atggcgtaat cgtatgtacg
     1561 ttcatcgccc ataacaccga cactgcgcat gttggttaag gccgcaaagt actgattgat
     1621 cgcacggcca agacctgcgg ctgcaacctc ctcacggaag atcgcatccg catcacggag
     1681 aatgtcgagc ttctccttgg tgatgtcgcc gatgacgcgg atggcaagac ccggacccgg
     1741 gaacggctgg cgccacacaa gatgttccgg aatgccgagc tcaacacccg cagcacgcac
     1801 ctcgtccttg aagagaatgc gaagcggttc gatgatttct ttgaagtcca catgatccgg
     1861 cagaccgccg acgttgtgat ggctcttgat gaccgcggca tttccgggac cggactcgat
     1921 gacatccggg taaatggtac cctgcacaag gaaatcaacc gttccgatct tttttgcctc
     1981 ctcctcaaag acatgaatga actccgcacc gatgatcttc cgtttctgtt ccggatcgga
     2041 aactcctgca agctttgcaa ggaacctatc ctcagcattt acgcggatca gattgatgtc
     2101 gtactggttt ctgaagacct cttcaacctc gtctccctcg tttttgcgca gaagaccgtg
     2161 atccacaaaa atgcaggtca gttgtttgcc gacagccttg tgcacaagga ctgcggcaac
     2221 cgatgagtcc actccgccgg aaagagcaca gagtactttt ttgtcgccga tcttttcccg
     2281 cagatgggca accatgttgt ccacaaacga ggacatgtgc caggttccag tacagccgca
     2341 gacaccgtac aggaaat
//
LOCUS       DALTZZ010000024         3752 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_3995,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000024 DALTZZ010000000
VERSION     DALTZZ010000024.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3995"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            154..1155
                     /gene="fen"
                     /locus_tag="O0X12_00390"
     CDS             154..1155
                     /gene="fen"
                     /locus_tag="O0X12_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HJK52651.1"
                     /translation="MGVALRPLIADYKETLGWDQLGGTAAIDAYNALYQFLSGIRQPD
                     GTPLMDDDGHITSHLSGLLFRTANLVEKNITPVYVFDGKPPAFKEATLAERRIIRENA
                     KDSWERAVKEGDEETARKYAMASSKVDAYVINSSKELLTALGIPWIQAPEEGEAQSAY
                     MAAAGDVTYAVSQDYDSLLFGTPKLLRNLTVSGKRRMHGKYVPVYPERLILEDVLNGL
                     GMSRVELIAAALLIGTDYNSGVKGVGPKTAVKIVRDGKFAERITESENAADPEDLICY
                     FLDPPVEKNYTIAKRSPVQEKVFEILCEEHGFSEERVSAGLERLGAKKGQATLDAWF"
     gene            complement(1158..1535)
                     /locus_tag="O0X12_00395"
     CDS             complement(1158..1535)
                     /locus_tag="O0X12_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HJK52652.1"
                     /translation="MTDCPFCNPDNILLENDLSFAKYDQYPVSPGHVLVMPKRHTASW
                     FDLTAEEQTAMFALASQAKKLLDERYAPAGYNLGINCGEAAGQTIMHVHLHIIPRYCG
                     DVPNPRGGIRAVIAAKQDYTHWV"
     gene            complement(1543..2406)
                     /gene="artA"
                     /locus_tag="O0X12_00400"
     CDS             complement(1543..2406)
                     /gene="artA"
                     /locus_tag="O0X12_00400"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HJK52653.1"
                     /translation="MLEQLIEAVVIFSCIAFLLFLIPGKHQKYVASVGWITMEAVLLL
                     MFPFFFEEGNFVYPAIAIVALPCIYITVKRLFMDDYYVRRLTYAASIAYLVYAPFAFI
                     PAIGNWLISVVEDLVAVVLTAIGTPFTMADWNIFRGEVLPGNTFGYQVEVILGRTGIQ
                     AIAILLGVVAIIPSSWKRKLGLFCLVTIPIFAVNIFRNAYVVTAYTQQWYPWFQEWFP
                     GPEMVGYASFFWAHNVFCELLALAVLVVIAYLLFRLNPTMVAVLREIGVVYYDELTAM
                     RDRLLGKKSTE"
     gene            2515..2835
                     /locus_tag="O0X12_00405"
     CDS             2515..2835
                     /locus_tag="O0X12_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HJK52654.1"
                     /translation="MMFCPKCGKLMKSKDGKLTCAKCGYAEEISSAEKEHMKKVAYMQ
                     ENDILIVDDDMEIETKPTCAIKCPNCGHGIAEWWLRQLRSADESEVRFFRCTKCKHTW
                     REYD"
     gene            2925..3485
                     /locus_tag="O0X12_00410"
     CDS             2925..3485
                     /locus_tag="O0X12_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HJK52655.1"
                     /translation="MSGLPDESLKIENVVASTKVNDKLDLLVLAAQIPGAEYNKQRFP
                     GVVLRMQNPKIAALVFGSGKVVLTGAKSIDSLNKGLEYLVGLLRDLNIEIVDNPTYSI
                     QNIVTSADLGSRINLNKIAMSFNLDKIEYEPEQFPGLVYRLDDPKVVVLLFGSGKLII
                     TGGKVPEDAKRAVQKIYQDLSDMKML"
BASE COUNT          983 a          890 c          990 g          889 t
ORIGIN      
        1 cttgaagagc agagcgcggc acgtgagcgg atcatgcgca gcaatcttga ggaccttggc
       61 cggcagatga ttcagaggca ggatgcaacc tcgcagaagg ttgttgaggc ggtcgagagc
      121 atcaaaagca gaatgtaccg gtaaggagat ttcatgggtg ttgcgctgcg gccgcttatt
      181 gcggactata aagagaccct tggatgggat cagcttggcg gtactgcggc cattgatgcc
      241 tacaacgccc tgtatcagtt tttaagcggc attcgtcagc cggacggcac accgctgatg
      301 gatgacgacg gccatatcac gtcgcatctc agcggtcttt tgttccgtac cgcaaacctt
      361 gtggaaaaaa atatcacgcc tgtgtatgtg tttgacggaa aacctccggc atttaaggag
      421 gctacccttg ccgaacgccg tatcattcgc gaaaatgcaa aggattcctg ggaacgtgca
      481 gtaaaagaag gggatgagga gactgcacgc aagtatgcca tggcatcatc aaaggttgat
      541 gcctatgtca tcaactcctc caaagaactt ctaacggccc tgggaatccc gtggattcag
      601 gcccctgagg agggagaagc acagtctgcc tacatggcag ccgcagggga cgtgacttat
      661 gctgtctccc aggactatga ctccctgctg tttggaacac cgaaacttct ccggaacctc
      721 acggtttcag gcaagcgccg tatgcacgga aagtatgtgc cggtctatcc ggagcggctg
      781 attttggaag atgtcctgaa cgggcttggc atgagccgtg ttgagttgat cgcagcagcc
      841 cttctcatag ggaccgacta caacagcggt gtaaagggtg tagggccgaa gactgcggta
      901 aagattgttc gtgacggaaa gttcgccgag cgcattaccg agtcggaaaa cgccgcagac
      961 ccggaggacc ttatctgtta cttccttgac ccgcctgtgg aaaagaacta caccattgca
     1021 aaaagatccc ctgtgcagga gaaggtgttt gagattctct gtgaagagca cggcttctcg
     1081 gaggaacgcg tgtctgcggg actcgaacgg ctcggtgcaa aaaagggcca ggcgaccctt
     1141 gatgcctggt tctgaggtca gacccagtgg gtataatcct gttttgccgc aattaccgca
     1201 cgtattcccc cgcggggatt tgggacatca ccgcagtatc ggggaatgat atgcagatgg
     1261 acatgcatga ttgtctgacc tgctgcctct ccgcagttta tgcccaggtt gtacccagca
     1321 ggcgcatatc gttcatccag tagttttttt gcctggctgg caagtgcaaa catggcggtc
     1381 tgttcttcag ccgtgagatc aaaccaggat gctgtatgcc gctttggcat caccaagaca
     1441 tggccgggcg agaccggata ctgatcatac ttggcgaatg acagatcatt ttccagaaga
     1501 atgttgtccg ggttacagaa tgggcagtct gtcataaaaa tattattctg ttgatttttt
     1561 accaagcagg cggtcacgca ttgcggtaag ttcgtcataa tacacaacac ctatctcacg
     1621 gagtaccgca accatggtgg ggttgagccg gaagagaagg taggcaatga cgaccagtac
     1681 tgccagtgca aggagttcgc agaacacgtt gtgtgcccag aagaaacttg catagccaac
     1741 catctctggt cctgggaacc attcctggaa ccaggggtac cattgctggg tatatgcggt
     1801 tacaacatat gcattgcgga agatgttcac tgcaaagatc gggattgtta ccaagcagaa
     1861 cagtccgagt ttcctcttcc agctcgacgg gatgatggcc actaccccga gaagaatggc
     1921 tattgcctga attccggtac ggccaagaat cacctctacc tgataaccga aggtgttgcc
     1981 cggcagaacc tcacccctga agatgttcca atccgccatg gtaaacggtg ttccaattgc
     2041 tgtcagaaca accgctacca gatcctccac tacgctgatg agccagtttc caattgccgg
     2101 aatgaacgca aacggtgcat acacgagata ggcaatagat gccgcgtatg tgaggcggcg
     2161 gacgtagtag tcgtccatga acagtcgttt tacagtgatg taaatacagg gcagggcaac
     2221 gattgcaatt gcgggataga caaagtttcc ttcctcaaaa aagaagggaa acatcagcag
     2281 gaggaccgcc tccatcgtaa tccatccgac actcgccacg tacttctgat gctttcccgg
     2341 aatcagaaac agcaggaatg caatacagga aaagatgact acagcttcga tcaactgctc
     2401 cagcataaga actggttaca ctgttagccg taatggatga atagggtttt gttgatatca
     2461 catgcctgtg ggatggtcag agtatataag gttccaagac aaatatttaa ctctatgatg
     2521 ttctgtccga aatgcgggaa actgatgaag tccaaagacg gcaagctgac ctgtgcaaag
     2581 tgcgggtatg cagaggagat cagctcagca gagaaggaac atatgaaaaa ggttgcttac
     2641 atgcaggaga acgacatcct catcgttgac gacgatatgg agatcgagac aaagccgacc
     2701 tgtgccatca agtgcccaaa ctgcggacat ggaattgccg aatggtggct tcgccagctc
     2761 cgcagtgcag atgagtcaga ggtccggttc ttccgctgta ccaagtgcaa acacacctgg
     2821 cgtgaatacg attaatactg tcattcagag agagtcatct ctcaactgat tttttctgcg
     2881 gcatcaaccg agtttttata tagttacctg ttttagatat aaatatgtca ggcctgcccg
     2941 atgagtcatt gaagatcgag aatgtggttg catccacaaa agtgaatgat aaactggatc
     3001 tcctcgttct cgcagcgcag atacccggcg cagaatacaa caaacagcgg ttcccgggtg
     3061 tcgttttgcg gatgcagaac ccgaaaattg cggcattggt attcggatca ggaaaggttg
     3121 tgcttaccgg tgcaaagagt attgacagtc tcaacaaagg tcttgagtac ctggtaggcc
     3181 tgcttcgtga cctcaacatc gagattgtgg acaatcccac ctacagtatt caaaatattg
     3241 tcacctccgc agatcttggt tcacgtatca accttaacaa gattgcaatg agctttaacc
     3301 ttgacaagat cgagtacgag cccgaacagt tcccgggcct tgtgtaccgg cttgatgatc
     3361 cgaaggttgt tgttctcctg tttggatcag gaaagctgat catcaccggc ggcaaggtcc
     3421 ccgaggatgc taaacgggcg gtccaaaaga tctaccaaga tctctctgat atgaagatgc
     3481 tttgatcttg ctgggctacc ggcatccctg aatctttttt gctcatctct gtgttactct
     3541 ataccgtcct gttctttggc acgtttaagc cgttgagatt acacaaatac agcggcaatg
     3601 ctataaattt atacggtgta taagtatagt attattaacg accgtcacac tggtatgttc
     3661 gggtgtgtca aggggtatta cagatgttaa aagcaacaat tgatgcagat attttccggg
     3721 agactatcga tgcggtctcc gcactggtga ac
//
LOCUS       DALTZZ010000025         1731 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_4101,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000025 DALTZZ010000000
VERSION     DALTZZ010000025.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4101"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            67..951
                     /gene="rfbA"
                     /locus_tag="O0X12_00415"
     CDS             67..951
                     /gene="rfbA"
                     /locus_tag="O0X12_00415"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HJK52656.1"
                     /translation="MKGIILAGGHGTRLHPITIAVSKQLLPIYDKPMVYYPLSTLLLA
                     GIRDILIISTPEDIDSYRLLLGDGSRLGVSFTYKVQNAPNGLAEAFILGEEFIGDDSV
                     CLILGDNVFYGQGFTDILQKAKKNTTGATIFGYPVANPSAFGVVECDPNGRVLSLEEK
                     PVNPKSKYAVPGLYFYDNDVVEIAKNVKPSARGEIEITSVNNEYLRRGKLNVILMGRG
                     MAWLDTGTPKNLYQASAFVEIVQERQGYYISCIEEIAWRRGFIDDEQLQKLGKEQEQT
                     AYGQYILSLLEEKGTKSL"
     gene            948..>1731
                     /gene="rfbB"
                     /locus_tag="O0X12_00420"
     CDS             948..>1731
                     /gene="rfbB"
                     /locus_tag="O0X12_00420"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HJK52657.1"
                     /translation="MKTYLVTGGCGFIGANFIHYIFRKYGEQIRILNLDKLTYAGDPK
                     NLADVSDRPNYTFIKGDICDKELVNRIFRDNLVDYVVHFAAESHVDRSIAAADAFVTT
                     NILGTFTLLDAAKRAWTCGETYREGVRYLHVSTDEVYGSLGPAGYFTETTPLDPHSPY
                     SASKASADLLVKSYFDTHRLPVNITRCSNNYGPYQYPEKLIPLLITNCLAHKPLPVYG
                     DGLNVRDWLYVEDHCRAIDLVLHHGVAGEIYNIGGHNEKTNIE"
BASE COUNT          447 a          464 c          395 g          425 t
ORIGIN      
        1 gtgttgaaca taataattat ggcgagtatt atctgtgtgc agaactaaac aatgtgtgtc
       61 ttacaaatga aaggcatcat cctcgcaggt ggtcacggca cccgcctcca cccaataacc
      121 attgcagtat ccaagcagct gctgcccatc tacgacaaac cgatggtata ttaccctctt
      181 tctactctcc tccttgcagg catccgcgac atcctcatca tatcaacccc ggaagacatc
      241 gactcttatc gtctcctcct cggtgacgga tccagattag gtgtctcctt cacctacaaa
      301 gtgcagaacg ccccgaacgg tcttgccgaa gccttcatcc ttggtgaaga gttcatcggg
      361 gacgacagcg tctgtctgat tcttggagat aacgtattct atggtcaggg gttcacggac
      421 atactgcaaa aagcaaagaa aaatacaacc ggtgccacta tcttcggata cccggttgcc
      481 aatccctcag ccttcggtgt tgttgaatgt gatccgaacg gtcgtgttct ttcccttgag
      541 gaaaaaccgg tcaacccaaa atcaaagtac gcagtccccg gtctttactt ctatgacaac
      601 gatgttgtgg agattgccaa aaatgtcaaa ccctctgccc gcggcgagat tgagatcacc
      661 tcggtaaata atgaatatct tcgtcgcgga aaactcaatg taattctcat ggggcgcggt
      721 atggcatggc tggacaccgg aacgccgaaa aatctctatc aggcaagtgc ctttgttgag
      781 attgtgcagg aacgtcaggg atattacatc tcctgcatcg aagagatcgc ctggcgccgc
      841 ggattcattg acgatgagca gctccaaaaa cttggaaaag aacaggaaca gaccgcctac
      901 ggtcagtaca ttctctccct ccttgaggaa aaaggaacaa agtctttatg aaaacctatc
      961 tcgtaaccgg cggatgcggc tttatcggag caaacttcat ccactacata ttccggaagt
     1021 acggagaaca gatccgtatc ctcaatcttg acaagcttac ctatgcaggt gatccgaaaa
     1081 atcttgcaga tgtctctgat cgtccaaatt atacctttat caaaggtgat atctgcgaca
     1141 aagagctggt gaaccggatc tttcgtgata acctggtaga ttatgtcgtt cactttgctg
     1201 cagagagcca tgttgaccgt tccattgctg ccgctgatgc atttgtgacg acaaacattc
     1261 ttggaacctt cacgctgctt gatgctgcaa agcgtgcctg gacctgcggg gaaacttatc
     1321 gtgaaggtgt tcggtatctt catgtctcca ccgacgaagt gtacggctcc cttggccccg
     1381 cggggtactt cactgagaca accccgctcg atccgcacag cccgtactca gcctcaaagg
     1441 catccgcaga cctgctcgtc aaatcctact ttgacaccca ccggctgccg gtaaacatca
     1501 cccgctgttc caacaactat ggcccgtatc agtatccgga aaaactgatt ccgctcctga
     1561 ttaccaactg tcttgctcat aaaccgctcc cggtgtatgg ggacggcctc aatgtccgtg
     1621 actggctgta tgtcgaggac cactgccgtg cgattgatct cgttctccat catggggtgg
     1681 ctggtgaaat ctacaacatc ggaggacata acgagaagac aaatattgag a
//
LOCUS       DALTZZ010000026         2618 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_4231,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000026 DALTZZ010000000
VERSION     DALTZZ010000026.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4231"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="O0X12_00425"
     CDS             complement(<1..887)
                     /locus_tag="O0X12_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HJK52658.1"
                     /translation="MELSEYLKSVAAKVDQEADEYSKATDTTLQKASAHLLLGGGKRL
                     RPAMVLLAADAVRKGASTDVFSAALALEWTHTFTLVHDDIMDGDSLRRGRPTVHVVWD
                     EPTAILAGDVLYAKAFEYLCSVKNASAESKVAAVQMLSHACYEICEGQQEDVSFETRN
                     DVTADEYLEMVRKKTGVLYAAAAGIGAALAGGDMKQVSAFYTLGLNTGIAFQIQDDII
                     DLMTPPEISGKDQASDLRENKQTILAITAREMGVDLSKYHKENLTKEEIAEAIALLEE
                     SGVLGKVRSISEKLIADSKA"
     gene            complement(911..2254)
                     /locus_tag="O0X12_00430"
     CDS             complement(911..2254)
                     /locus_tag="O0X12_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase J family beta-CASP ribonuclease"
                     /protein_id="HJK52659.1"
                     /translation="MVDIEVIAVGGYNEVGRNMTAVRVGKEIVIFDMGLYLDPISGNN
                     NVEMENMHSLDLIQIGAIPDDTVMNSVEGTVKAIVCTHGHLDHIGAVQKLAHRYNCPI
                     IATPYTTELIKQQIEGERKFSVTNKTFALKAGGKYTISQHLTLEFVRVQHSIIDSVMA
                     VVLTAAGCVVYANDFKLDMTPVIGEAPDLARMRAIGKEGVKVLIVESLNLDDRGYAPS
                     EQVARLRVRDAITRCGDDKNAIIVSTFASQIARIKTITECAREIDRIPILLGRSMERY
                     NGTAEMMKQVAFPQGTSIYGNRKTTDRMLRRVAKEGKDKFLLVVTGHQGEPGSMLSRM
                     ASGDTPFKIEKGDKVMFSANIIPNPVNYAQRAEVDRLLLRQGARIFDGLHVTGHAFYQ
                     EHYDLISMLNPEHIVPSHGPFDMKGGYVTLGREFGYALNKEIHIMQNGDRLVLPK"
     gene            complement(2291..>2618)
                     /locus_tag="O0X12_00435"
     CDS             complement(2291..>2618)
                     /locus_tag="O0X12_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HJK52660.1"
                     /translation="TWGVPTAVSVFEVARTGTGPVIATGGLRSGLDIAKGIALGATLG
                     GMALPLLSPALCGEDETYRTIDAIHYQLRAAMFLTGSPDVSALQHARTYITGTLREMV
                     GPQPNM"
BASE COUNT          589 a          751 c          633 g          645 t
ORIGIN      
        1 gcctttgaat ccgcgatcag tttttcggag atgctgcgga ctttcccgag gactcccgac
       61 tcttcaagaa gtgcaattgc ctctgcaatc tcctcttttg taagattctc tttgtgatac
      121 ttggaaagat ccacgcccat ctcgcgggca gtaatggcca gaatcgtctg tttgttctcg
      181 cgcagatcgg atgcctgatc ttttccggag atctccggag gcgtcatcag atcgatgatg
      241 tcatcttgga tctggaacgc gataccggtg ttcaggccaa gcgtgtaaaa cgcagagacc
      301 tgcttcatgt ctccaccggc aagtgctgca ccaattcccg cagcagccgc atacagaacg
      361 ccggtcttct ttctgaccat ctcaagatac tcgtctgcgg taacatcatt ccgggtctca
      421 aaggacacat cctcctgctg accttcacag atctcataac aggcgtgcga cagcatctgg
      481 accgctgcaa cctttgactc ggcacttgca ttcttcacag aacacaggta ctcaaacgcc
      541 ttcgcataca aaacatcgcc cgcaagaatt gccgtcggtt catcccagac cacatgcaca
      601 gttggtcttc cacggcgcag tgagtccccg tccatgatat cgtcatgcac cagcgtgaac
      661 gtatgtgtcc actcaagggc aagagccgcg gagaaaacat ctgtggatgc acctttgcgg
      721 acagcatctg ctgcaagcag aaccattgcc ggacgaaggc gttttcctcc gccaaggagc
      781 agatgcgcgg atgctttctg cagggttgtg tcagtcgctt tgctgtactc gtctgcctcc
      841 tgatcaactt tcgcggcgac ggatttcaga tactcggata actccataat acacactctc
      901 aattttttgg ttatttcgga agaaccaaac ggtctccgtt ctgcataatg tgaatctctt
      961 tgttcagcgc ataaccgaac tcgcggccaa gggtcacata cccgcccttc atatcgaacg
     1021 ggccgtgtga cggaacgata tgttccggat tcagcatcga gatcagatca taatgctcct
     1081 gataaaatgc gtgacccgtc acatgaagac cgtcaaagat tcttgcaccc tgccgcagca
     1141 gaagccggtc aacctctgca cgctgggcat agttcaccgg atttggaatg atgtttgccg
     1201 aaaacatcac cttatcgccc ttctcgatct tgaacggggt gtcaccggaa gccatgcggg
     1261 aaagcatcga acccggctca ccctggtgac cggtaaccac aagcaggaac ttgtcctttc
     1321 cttcctttgc cacacggcgc agcatccggt cagtcgtctt gcggtttccg tagatgctcg
     1381 ttccctgcgg gaaggcaacc tgcttcatca tctcggcggt tccgttgtac cgctccatag
     1441 atcttcccag aagaatcggg atgcggtcaa tctcgcgggc acactcggtg atggttttga
     1501 ttcttgcaat ctgtgaagca aaagtcgaga caatgatcgc gttcttatca tccccacaac
     1561 gcgtgatcgc atcacgcacc cgcagacggg cgacctgttc gctcggagca tacccgcggt
     1621 catcgaggtt cagcgactca acaatcagaa ccttcacacc ctccttgccg atagcacgca
     1681 tgcgtgcaag gtcgggtgct tcgccgatca ccggtgtcat gtccagtttg aagtcgtttg
     1741 catacacgac acaacccgca gccgtgagaa ccaccgccat gacagagtcg ataatactgt
     1801 gctggacacg gacgaactcc agcgtcagat gctgggagat ggtatatttg ccgcctgctt
     1861 tcagcgcgaa cgttttgttc gtcaccgaga acttccgctc gccctcaatc tgctgtttaa
     1921 tcagttcggt cgtgtacggg gttgcgatga tcgggcagtt atagcggtgg gcaagtttct
     1981 gtactgcacc aatgtggtca aggtgaccgt gcgtacaaac gatcgcctta accgtccctt
     2041 cgaccgagtt catcacggtg tcgtcgggga ttgcaccaat ctggatcaga tccagcgagt
     2101 gcatattctc catttctaca ttgttgttcc ctgagattgg atcaagatac agacccatat
     2161 caaagataac gatctcttta ccgacgcgga ccgcggtcat attccttccg acctcgttgt
     2221 atccgccgac ggcgatgact tctatgtcaa ccattgtgtt atttctccga attttcagac
     2281 cttaggacat tcacatattt ggttgcggcc caaccatttc ccgcaatgtt cccgtaatat
     2341 atgttcttgc gtgctgaaga gcggaaacat caggagatcc ggtcagaaac attgctgcac
     2401 gaagctggta gtgtatggcg tcaatggttc tgtatgtctc gtcctctccg cacagtgcag
     2461 gagagagtag aggaagtgcc atacccccaa gtgttgcccc gagcgctatt ccttttgcga
     2521 tgtcaagacc cgagcgcaga ccgccggttg cgatcacagg tcctgttcct gtcctggcta
     2581 cctcaaaaac acttacagca gtaggaaccc cccacgta
//
LOCUS       DALTZZ010000027         2082 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_4253,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000027 DALTZZ010000000
VERSION     DALTZZ010000027.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4253"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="O0X12_00440"
     CDS             <1..399
                     /locus_tag="O0X12_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HJK52661.1"
                     /translation="IQAGADIAVGSRFLEESTVLRSGDREMTSVGFNTMVHLLLGSNI
                     RDHQCGFKAFRRDRLAKLTPYVQARGWTWDTEVLAIGQRCGYTIEEIPITWTEGEKTN
                     IRTRDIFSMGWAVLGLAWRIRITGKYPKDV"
     gene            551..919
                     /gene="rpl7ae"
                     /locus_tag="O0X12_00445"
     CDS             551..919
                     /gene="rpl7ae"
                     /locus_tag="O0X12_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HJK52662.1"
                     /translation="MAKIYQMFEVPEELQNKALEALELARDTGKIKKGANEATKAVER
                     GTAALVLIGADVAPEEIVMHIPGLADEKEIPFVFINKQADIGAACGLDVGCTAVAIVK
                     VGKGKEIVEDLAGQIKALRG"
     gene            925..1134
                     /locus_tag="O0X12_00450"
     CDS             925..1134
                     /locus_tag="O0X12_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HJK52663.1"
                     /translation="MPDDATPAEVIEVIGLTGMHGEAAQIKCRVLDGPNKGRIITRNT
                     FGPIREGDILMLLETEREAKKLSRR"
     gene            1142..1333
                     /locus_tag="O0X12_00455"
     CDS             1142..1333
                     /locus_tag="O0X12_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HJK52664.1"
                     /translation="MVDIYTCSYCGKQLEPGTGKLFVRKDGAVFYFCSSKCQSNYNLG
                     RIPRRVAWTAAGRKARGKE"
     gene            1338..1787
                     /gene="ndk"
                     /locus_tag="O0X12_00460"
     CDS             1338..1787
                     /gene="ndk"
                     /locus_tag="O0X12_00460"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HJK52665.1"
                     /translation="MERTFVMIKPDGVQRGLVGEILSRFEKKGFKIVAAKFGVLPEAI
                     VDKHYEEHLAKPFYPGMKAYITSGPVFRFVLEGDNVIATVRKMNGATNPAEAAPGTVR
                     GDYALSIGKNVIHASDAPESAEREINIHFTAEELVAYEKIDESQLYE"
BASE COUNT          571 a          502 c          543 g          466 t
ORIGIN      
        1 atccaggcag gtgcagacat tgcagttggg tcacggttcc ttgaagaaag taccgtactg
       61 cgttcaggcg atcgtgagat gaccagtgtt ggattcaata cgatggtaca tctgctcctt
      121 ggaagcaata tccgtgatca tcagtgcgga tttaaggcat tccgccgtga ccgactcgca
      181 aaactgactc cgtatgtgca ggcgcgcggt tggacctggg acactgaggt tctcgcaatt
      241 ggacaaagat gcggttatac cattgaagag atcccaatca cctggactga gggtgaaaaa
      301 acgaatatcc gaaccagaga cattttttcc atgggttggg cggtactggg acttgcatgg
      361 aggatacgca ttacaggcaa gtacccgaag gatgtctaac ctgcagatcc taaggttctg
      421 tccaaacctt tatgccaaac tctgacccat atatagagtt acgccagcaa cggtctgtta
      481 ccttaccagg agagccgctg atgagcgatg acggatggtc agagaaccgt cccgagtgaa
      541 gtgagtcatt atggcaaaga tttaccagat gtttgaagtc cctgaagaac ttcagaacaa
      601 agcacttgaa gctcttgagc ttgcacgtga caccggaaag attaagaaag gcgcaaacga
      661 ggcaaccaaa gccgttgagc gcggaaccgc agctctcgtc ctgatcggtg ctgatgttgc
      721 acccgaagag attgtcatgc acattccggg acttgcagac gaaaaagaga ttccgttcgt
      781 ttttatcaac aaacaggcag acatcggtgc tgcatgcggc cttgacgtcg gctgcactgc
      841 tgttgcaatt gtaaaagtcg gcaaaggcaa ggaaatcgtt gaggaccttg caggccagat
      901 caaggcactc agaggataaa taccatgcct gatgatgcaa cgccagcaga agtcattgag
      961 gtcatcggcc ttacgggtat gcacggtgag gcagcccaga tcaagtgccg tgtccttgac
     1021 ggcccgaaca aggggcggat cattacccgg aacaccttcg gtccgatccg tgagggtgac
     1081 atcctgatgc tccttgagac agaacgtgaa gcaaagaagc tctcaagacg gtgagtaaaa
     1141 gatggtcgat atctatacct gcagctactg cggcaagcag cttgaaccgg gaaccggtaa
     1201 actgttcgtc agaaaggatg gtgcagtctt ctacttctgc tcctccaaat gccagagcaa
     1261 ctacaacctc ggtcgtatcc cgcgccgtgt tgcctggacg gcagccggac gcaaggcacg
     1321 cggcaaggag taaaatcatg gagcgcacct ttgttatgat caagccggac ggcgtccagc
     1381 gtggtctggt cggtgagatc ctttcccgct tcgagaagaa gggattcaag atcgtcgccg
     1441 caaagtttgg cgtgcttcct gaagcaattg tggacaagca ttacgaggag cacctcgcaa
     1501 agccattcta cccaggtatg aaggcataca tcacctccgg ccctgtgttc cgctttgttc
     1561 ttgaaggtga caatgttatt gcaactgttc gcaagatgaa cggtgcaact aacccagcag
     1621 aagcagcacc cggaaccgtc cgcggtgact atgcattgtc catcgggaaa aatgtaattc
     1681 atgcgtcaga tgcacccgag agtgcagaac gcgagattaa cattcacttt actgcagaag
     1741 agcttgtcgc atacgaaaag atcgacgagt cacagctcta cgagtaaaca tccggtattt
     1801 ttccggaacc atttttttaa acagattgta ccgacttgtc cgaatggtaa gggactatcg
     1861 cagaatcatt ccagagatat tgtgttaaaa taaaagagaa ggggattatt ttctcagata
     1921 gtcatacgcg gaaagtaatg cctttacacc ttcgccaacg gaggaagcaa tctgttttgc
     1981 tgcaaccgag gtcgaatctc cagctgcaaa gaaaccagga acactcgttt tacagtcaat
     2041 atccacaatg atctcctttt ctgcattcat gggagcaaaa cc
//
LOCUS       DALTZZ010000028         2599 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_4288,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000028 DALTZZ010000000
VERSION     DALTZZ010000028.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4288"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="O0X12_00465"
     CDS             <1..489
                     /locus_tag="O0X12_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HJK52666.1"
                     /translation="LPVEPRMRISIECDGRPMGSALNEAVIVTSRPAKMLQFETFING
                     RKSTEFRADGLIVGTPTGSTAYAMSAGGPIVDPSIEAFVLVPLAPFMLSSRPHLFSSS
                     SEIEVRLISSKPSQLVLDGQVQYDIGEEASLVVRKSPEPALFLDVGRSFFEKVEQKLR
                     LL"
     gene            643..1350
                     /locus_tag="O0X12_00470"
     CDS             643..1350
                     /locus_tag="O0X12_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HJK52667.1"
                     /translation="MDGMKSNPDFAAISAVLKENLDLVGSPVAVKIVTAPEQIPAGVP
                     EIEETVRHCRMISLAREGKVFYATDAKHQCGGGAWALGLREKGATLKSGEHYYKLGKY
                     ESLASSRRTMASVPNLPQETYATMYAPLEKAEFAPDIVMIFAKPKSMLKLAQSVLFRL
                     GGRIYPQFSGIQSVCSDATAYVLLSGKPNFSLGCDGSRKFSGIADEEMVVGFPVEMIE
                     EVAAILPTVVNAAGSKK"
     gene            complement(1429..2049)
                     /locus_tag="O0X12_00475"
     CDS             complement(1429..2049)
                     /locus_tag="O0X12_00475"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJK52668.1"
                     /translation="MTDDNVVVVEQGSFEAQKIAKAMSSPTSADLFNALSDYPMSATA
                     LAERCELPLTTVKYHLENMMSAGLIEVTDTRWSAKGREMKIYAVKDQVVVIAPRKKVD
                     IRGIVERYGVAAGALAIVCSLGLAIPSTLLGFVQGTPGGVTGVTPALVKSGFDALATT
                     SVAETTASAATMPLWLHEAVMVFFVAGVVILAMMMVYEVYSMRRDI"
     gene            complement(2200..>2599)
                     /locus_tag="O0X12_00480"
     CDS             complement(2200..>2599)
                     /locus_tag="O0X12_00480"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833639.1"
                     /note="catalyzes the removal of N-terminal amino acids
                     from peptides and arylamides; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II methionyl aminopeptidase"
                     /protein_id="HJK52669.1"
                     /translation="YDLHHGLSIPNIGLFGSGVLRKDDVIAIEPFATTGSGMVHEAPR
                     AEIYQVVGNAPVRSPTARKIMKKAQEMNGLPFSRRWLQIPKADLALPTLLRQGNLYVH
                     HCLADVPESFVAQFEHTMIVTDDGALVTTR"
BASE COUNT          521 a          611 c          789 g          678 t
ORIGIN      
        1 ctgccggttg agccgcgtat gcgtatttcg attgagtgcg acggaagacc gatgggttct
       61 gcgctgaacg aggcggtgat cgttacgtcg cggccggcga agatgctgca gtttgagacg
      121 tttatcaacg gcagaaagtc caccgagttc cgtgcggacg gtcttattgt tggtacaccg
      181 accgggtcaa cggcgtatgc gatgagtgcg ggcgggccga ttgtggatcc gtccattgag
      241 gcgtttgttc tggtgccgct cgctccgttt atgctgtcgt cgcggccgca tctgttcagt
      301 tcgtcgagtg agattgaggt gcggcttatc agcagtaagc cgtcgcagct ggtgcttgac
      361 gggcaggtgc agtacgatat tggtgaggag gcgtcgcttg tggtgcggaa gtcgccggag
      421 cctgctctgt tcctggatgt ggggcgaagt ttctttgaga aggttgagca gaagctcaga
      481 cttctgtgat ctttttttcc tgattgttcg tatgcggtgt cagcggtgtt ctgtattttt
      541 tcagaactaa tcatatactt gcatatggtt tatgtctggg ttttaccggc acctcggttc
      601 tggaaacgaa taattatata accgcatccc ctatgtttca tcatggatgg tatgaaatca
      661 aatccggatt ttgcagcaat ctctgctgtt cttaaggaaa acctggatct ggtcggatca
      721 ccagttgcgg tgaagatcgt gactgcaccc gagcagattc cggccggagt gcctgagatt
      781 gaggagacgg ttcgccactg ccgcatgatc tctcttgccc gtgaggggaa ggtcttctat
      841 gcaaccgatg caaagcacca gtgcggcggc ggtgcctggg ctcttggtct tcgcgagaag
      901 ggtgcgacac tgaagtccgg tgagcactat tataaactgg ggaagtatga gtctcttgca
      961 tccagccgca gaaccatggc gagtgtgccg aatcttccac aggagacgta tgcgacgatg
     1021 tatgccccgc tggaaaaggc agagtttgcc ccggatattg tgatgatctt tgcaaagccc
     1081 aagtcgatgc tcaaacttgc acagtcggtg ctgttcagac tcggcggcag aatttatccg
     1141 cagttctccg gaattcagtc ggtctgttcc gatgcgaccg catatgttct tctgagcggt
     1201 aagccgaact tctctcttgg ctgtgatggt tcccgcaagt tctccggtat tgccgatgag
     1261 gagatggtgg tcgggttccc ggttgagatg attgaggagg ttgccgcaat tctgccgacg
     1321 gttgtcaatg cggcaggctc caaaaaataa tcttttttat actgttttcg tgggccaaga
     1381 gacgccgggt gtgttctggc gtttgggatt ttgttgaaaa aagagggttt agatgtcacg
     1441 cctcatggag tagacttcat acaccatcat cattgcaagg attactactc ccgcgacaaa
     1501 gaataccatg actgcttcat gaagccagag cggcattgtg gctgcggatg ccgttgtttc
     1561 cgcaacactc gtggtggcga gtgcgtcaaa tccgcttttt accagtgccg gcgttacgcc
     1621 ggtcacgccg ccgggtgttc cctgtacgaa tccaagcagt gtggagggta ttgcaaggcc
     1681 taaggagcag acgattgcaa gcgctcccgc cgccacaccg taacgctcca caataccgcg
     1741 gatgtccact ttctttctgg gtgcgatgac cactacttgg tctttgactg cgtagatctt
     1801 catctcacgg ccttttgcac tccagcgggt gtccgttacc tcgatgagac ctgccgacat
     1861 catgttttcc agatggtact ttacggtcgt caggggaagt tcgcaccgct cggctaatgc
     1921 ggttgcactc atggggtagt cagaaagggc gttgaacaga tctgccgagg tgggggaaga
     1981 catggctttt gcgatcttct gtgcctcaaa ggatccctgt tctacaacca caacattatc
     2041 gtctgtcacc gttatcacga cctgtaggag atgccccgtt tccggggtct gggcagcacg
     2101 tctggcgtgc gattcttacc agaacattgg acgcagaatt atataacctt ctggtaaact
     2161 tcgaagattg ttgcagttat gatttgccaa aaaaaggtgt tagcgggtgg tgacgagtgc
     2221 tccgtcgtca gttacgatca tggtgtgttc aaactgggcg acgaagcttt ccgggacgtc
     2281 tgctaagcag tgatggacgt agaggtttcc ctgtctgagg agagttggca gagcgaggtc
     2341 tgcttttggt atctggagcc atctgcggga gaagggaaga ccgttcatct cctgggcctt
     2401 cttcatgatt ttgcgggcag tcggggagcg gacgggagcg ttgccaacaa cctggtagat
     2461 ctctgcacgc ggggcttcgt ggaccatgcc ggatccggtg gttgcgaagg gttcgattgc
     2521 gattacgtcg tctttgcgaa ggacgccgga gccgaacagg ccgatgttgg ggatggagag
     2581 gccgtggtgg aggtcgtaa
//
LOCUS       DALTZZ010000029         1951 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_4615,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000029 DALTZZ010000000
VERSION     DALTZZ010000029.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4615"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            240..476
                     /locus_tag="O0X12_00485"
     CDS             240..476
                     /locus_tag="O0X12_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52670.1"
                     /translation="MSSTRTSYYLSLATVTAAAGLAAVLIVLGWSVMFGYCSLTVESF
                     HLLSLLVLGTAGSLVLLILLYFRFVVHQTSSHPS"
     gene            489..1079
                     /locus_tag="O0X12_00490"
     CDS             489..1079
                     /locus_tag="O0X12_00490"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HJK52671.1"
                     /translation="MNEVEIQSKIKQNAKNVGIVLTIGKILVVFVIVLLFLTAALTAC
                     IYTVGGPAEFSHWIVSEDVLSLLGVVTSVPAGMDPTTAFIQASAIFLISATVYLLTFA
                     GMIWAAARIFADIRENGVPFTQANARSLKHVAICMALLCVVPVITDSIGWYLTGISVT
                     LGYLSIEGLIVAAVLYCLAHIFEYGTLLQKQADETL"
     gene            1066..1302
                     /locus_tag="O0X12_00495"
     CDS             1066..1302
                     /locus_tag="O0X12_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HJK52672.1"
                     /translation="MKLSEVPMPIILRLDRVMADRKMSLNELSEKVGVTNVNLSKIKT
                     GKISAIRFSTLEAICRVLNCQPGDILEYQPEEKS"
     gene            1422..>1951
                     /locus_tag="O0X12_00500"
     CDS             1422..>1951
                     /locus_tag="O0X12_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HJK52673.1"
                     /translation="MITVGLLGCGNIGRVIAAGQENFKITAVYDVVPARVEEFAREFN
                     AKPFSDFSAFLAEPTDIIVEAASVVAATEHAQEVLESGKDIVIMSVGALADKEFRAHL
                     VETARSLKRKIHIPSGAVMGLDNIRIGQISRVDKFVLRTTKNPKSLSREATERTQLFS
                     GKAHDCVRQYPKNTNVA"
BASE COUNT          468 a          474 c          452 g          557 t
ORIGIN      
        1 ccggtacgga tatttgttgc cccgagatcg acggcagcaa catgcaggtt ttccgtcatt
       61 ttgccattac tatattttac ctccctcgct gatgaactat tccttcacga tacattgtat
      121 gttactcaga tattattatc gataagcgat aatttattaa tgtttgtatg acatactact
      181 atggtggaga tgcagaggta tcccttcact ctgcatcgcc ctgaccggag atctggaaaa
      241 tgtcatccac tcgcacgtcc tattacctgt cacttgcaac ggtcaccgct gcagctggtc
      301 ttgctgcagt tctgattgtt cttggatgga gtgtgatgtt tgggtactgt agtctgacag
      361 tggaaagttt ccacctcctt tccctgttag ttcttggaac agcggggagt ttagttctcc
      421 tcattctcct gtatttccgt tttgtcgtac atcagacctc ttcccatcca tcctaaacgg
      481 ggtgtcacat gaacgaagtt gaaattcagt caaaaataaa acaaaacgca aagaatgttg
      541 gcattgtcct aacgatcggc aagatcttag tggtttttgt gatcgtactg ctcttcctta
      601 ccgctgcgct taccgcatgt atctatactg taggaggacc tgcagaattc tcacactgga
      661 tcgtttccga ggatgttctc agtttattag gggtagtaac atctgttccc gctggaatgg
      721 atccgacaac cgcatttatc caggcatcag ctatattcct gatatcagct acagtttatc
      781 ttctaacctt tgccgggatg atctgggcgg cagcgaggat ctttgcggat atccgcgaaa
      841 acggtgtgcc gtttacccag gctaatgccc gcagtctgaa acatgtggct atctgtatgg
      901 cccttctgtg tgtggttccg gtcatcaccg actcaattgg ttggtatctt acgggaatca
      961 gcgttacact tggctatctc agtattgaag ggctgatcgt tgctgcggtt ttatactgtc
     1021 ttgcacacat atttgaatac ggaacactcc tgcaaaagca ggctgatgaa actctctgag
     1081 gtacccatgc ccattattct ccgacttgat cgcgttatgg cggacagaaa gatgtcgtta
     1141 aacgaacttt ccgagaaggt cggcgtgacc aatgtcaatc tttccaagat caaaaccggc
     1201 aagataagtg caatccggtt ctccaccctg gaagcaatat gcagggtact caattgtcag
     1261 cctggtgata ttctggagta tcagccggaa gaaaaatcct gatttggatt tttcatctcc
     1321 ccattttgtt tttaatcaca tcttttcaac atcactatca ttccccggag gtccggggtt
     1381 tggcataatc cttaactacg tacactctga tatttagtag tatgatcacc gtcggtctgc
     1441 ttggatgcgg aaatatcggc cgcgtcattg ctgcaggtca ggaaaacttc aaaataacgg
     1501 ctgtgtatga tgtggttccg gcacgtgtgg aggagtttgc tcgcgagttc aacgcaaaac
     1561 cgttttcaga cttttctgct ttccttgcag aaccaaccga tattattgtt gaggctgcat
     1621 ccgttgttgc tgccaccgaa cacgcacagg aggtgctgga gtcaggcaaa gatattgtca
     1681 tcatgagtgt cggggcactc gcagacaaag aattccgggc acatcttgtg gaaaccgccc
     1741 gcagcctgaa gcggaagatc catattccct ccggtgcagt gatgggactt gacaacatcc
     1801 gtatcggcca gatcagccgc gtcgataagt ttgtgctccg tacaaccaag aacccaaagt
     1861 cgctttcccg cgaagcaacc gaacgtacac agctgttctc cggcaaagcc cacgactgtg
     1921 tcagacagta tccgaagaac acgaatgtgg c
//
LOCUS       DALTZZ010000030         5038 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_4625,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000030 DALTZZ010000000
VERSION     DALTZZ010000030.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4625"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="O0X12_00505"
     CDS             <1..348
                     /locus_tag="O0X12_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52674.1"
                     /translation="PERHAALILLGVITALAIFHFGVVFLFPDGGAVPYRNDSADGSL
                     VTLTGTVEEITITKTGGHLILNISGTSVFVPGGASNLELLRGDNVTVRGTVETYAGEK
                     EIVVNNPAQIVLN"
     gene            511..939
                     /locus_tag="O0X12_00510"
     CDS             511..939
                     /locus_tag="O0X12_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52675.1"
                     /translation="MPEEKYITMTELPKTGERVDVLVLRELRQTERINVYLYMDEEIA
                     RTSDLAADLAKYRLVDPDFRPVMEIEEWFKTMQKQMRQIAGSNVRVEEMFAAEPLNAE
                     ILESGEIQTAVGPRTYLKALLPYLDEMEEMHYSNATMPTR"
     gene            complement(909..2165)
                     /locus_tag="O0X12_00515"
     CDS             complement(909..2165)
                     /locus_tag="O0X12_00515"
                     /EC_number="2.8.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase"
                     /protein_id="HJK52676.1"
                     /translation="MDSTALTAAALNQLRSIRLYAETYGCTYNFGDTEKIIEIARSQG
                     CTIVETAEDADAVLINTCIVVTRTEEHMYERMRLYADKILVVTGCLPGLSIDCVLSVH
                     PDAYIIDPDLIHSCYREVGTVHAGDHAVLQIAKGCNGHCTYCITRLARGKLVSFPLED
                     IVDQARAFVNAGVTEIQLTAQDVSSWGMDRDDGLRLPDLLSALIKIPGEYHIRIGMAN
                     PDTLLPILDDFLDAIADPKIFLFLHVPVQSGSDAVLRAMGRRYTAAQYEEICRRARER
                     YPDIRISTDYIAGFCGETAEDAAASVTQILTTRPGKVNITRFSMRPGTPAAKMKNLPE
                     PVKKERSRALTNAANAVYDANNEEWIGKTVDAVVTEVVRAGSVTARDRTYQNIVILSE
                     IPIGTRIRVTITGHRRHYLVGMVALE"
     gene            2247..4346
                     /gene="ppk1"
                     /locus_tag="O0X12_00520"
     CDS             2247..4346
                     /gene="ppk1"
                     /locus_tag="O0X12_00520"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HJK52677.1"
                     /translation="MGKKHKKEKKSNKEKKISRAEKPLCENCGRYFNRELSWLKFNER
                     ILTEARNPQNPLLERVAFLGIVADNLDEYFMVRVPAYQGGATRPSDEYEEVVGSRTQL
                     EMIYDRTIFLMRNISWIWAKELKPELEKNGILFTKYTKCSESEKKTLRKELSEILAKI
                     PVTVIRGDRFEDIEHNEYLKGLGLLAKSDRGFAVVPIQEILDTYGRFVRVGKRKTSYM
                     FREEQLRKNSDLFLPDEAFRAVVPVRLTRDSDLNLKGDDADDLISAIKAAPETLAKKL
                     PSRLETEQWLPFGYTSYLVNALSLLPELVYDVPSPLGLADLKKFPVSRPELKFEPYTP
                     ALPTGLSEVNRIFEHLADRDYMLFTPYDSFDGLVNFLNAAATDPAVKTIQMTLYRLGP
                     ESPVADALISAAKNGKDVTAVIELKASFDEEANFQWATKLSEGGVTVIHGVPGLKVHA
                     KCCLVTRAEGNKTVRYATISTGNYNAKTAKIYSDISLFTADPGICHDVATLFSYLNGN
                     EEHPEYKHLLVSPDFMEDSLKHLIRREIEHQKRSGNGHIVLKINSLTHRPIINALYEA
                     SNEGVKIDLIVRGICMLRPGVPNLSENIRVISIVGRFLEHSRVFYFRNNGDEQVFIGS
                     PDMMSRNLKRRVEIICPVLDRKIKNAIINRILPAFIHDTAKGHLLTSDGIYLPPKHPE
                     SNLGAQQMFITMRKVWW"
     gene            4340..4939
                     /locus_tag="O0X12_00525"
     CDS             4340..4939
                     /locus_tag="O0X12_00525"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HJK52678.1"
                     /translation="MVDLNKQELQTIHDAAVVLTEDFHHAEQVCRLSLAIYDDLSSAH
                     GYGKRTRRLLAAAALLHDIGWSISTVSHNKCGMTFIIEDRMLPLSLYERILVALAVRY
                     HRGGTPKRRHNLYGNLSKHDRQKVCLIAGIIRIADGLDRSHRSVVRSVHAKLSDETLV
                     LLCTGYGRGGPERRASARKADLLMKALSAKIQICWKRCP"
BASE COUNT         1432 a         1277 c         1131 g         1198 t
ORIGIN      
        1 ccagagcggc atgccgcact tattctctta ggcgtcatca cagcccttgc catcttccat
       61 tttggggttg tattcctttt tcccgacgga ggagcagtcc cttacagaaa cgacagtgcc
      121 gatggatcac ttgtcacact tacgggtaca gtggaagaga taaccatcac caaaaccgga
      181 ggacatctta ttctcaatat atccggcacg tccgtatttg tacccggggg tgcttcaaac
      241 ctagaacttc tgcggggaga caacgtaacg gtgcgcggta ccgtagagac atacgcagga
      301 gaaaaagaga tcgtagtaaa taatccagca cagattgtcc tgaattaaat gaaaataaat
      361 cagggtaaaa gactcccatc caaacagagg cacccaaatg gcaaaacccc cactattgaa
      421 acagaaatac gaatcatgag tccacctgca tgaattctca taaacgtatt ttgaaaagag
      481 aaaccagtca acccgtaaca ataaagtcat ttgcctgagg aaaagtacat caccatgaca
      541 gagcttccca aaaccggcga acgcgttgat gtacttgtac ttcgcgagct ccgccaaaca
      601 gaacgcatca atgtttatct gtatatggat gaggagatcg cacggacatc agatcttgct
      661 gcagatcttg caaagtaccg gttggttgac cccgactttc ggccagtaat ggaaattgaa
      721 gagtggttca agaccatgca gaaacagatg cgccagatag caggttcaaa cgtacgggtg
      781 gaagaaatgt ttgccgcaga acctctaaac gctgaaattt tggagagcgg agagatacag
      841 acagctgtcg gtccccgcac ctatctgaaa gcactcctcc cctaccttga tgagatggaa
      901 gagatgcatt actccaacgc caccatgcca acaagataat gccgacgatg gccagtgatc
      961 gttacccgta tccgagtacc tatgggaatc tccgataata tcacaatgtt ctgatatgtt
     1021 ctgtcgcgtg ccgtaacact tccagcacgt acaacctcgg tcacaaccgc atccactgtt
     1081 tttccaatcc actcctcatt gtttgcatca tacaccgcat ttgcagcatt cgtcaaggct
     1141 cgcgaccgct cctttttaac cggttcaggg agatttttca tctttgctgc aggagtgccc
     1201 gggcgcatcg aaaaccttgt tatgtttacc tttccgggac gggtagtaag aatctgtgta
     1261 actgatgctg ccgcatcctc tgcggtctca ccacagaacc ctgcaatgta atccgttgat
     1321 atgcggatat cagggtatcg ttcgcgggcg cggcggcaga tctcttcata ctgtgccgcc
     1381 gtgtacctcc tccccattgc ccggagtacc gcatctgacc ctgattggac aggcacatgc
     1441 aagaacaaaa agatctttgg atcagcaatt gcatctagaa aatcatcaag aattggcaac
     1501 agtgtgtcag gatttgccat ccctatacgg atatggtact cacccgggat ttttatcaga
     1561 gcactcagga gatccggtaa acgaagacca tcatcacgat ccatccccca agaactaaca
     1621 tcctgtgctg tcagctggat ctcggtcacg cctgcattca caaacgcacg cgcctgatcc
     1681 acaatgtcct caagaggaaa gctcaccagc tttccccggg caagtcgggt aatgcagtag
     1741 gtacagtggc cattacaccc ctttgcgatc tggagaactg catgatcccc tgcatgtacc
     1801 gttccaacct ctcggtaaca ggagtgaatc agatccggat caataatata tgcatccgga
     1861 tgtaccgaca acacgcagtc tattgaaagc cccggcagac agccggtcac aaccagaatt
     1921 ttatcagcat acaaacgcat gcgttcatac atgtgttcct ctgttctggt taccacaata
     1981 caggtgttga ttaaaaccgc atcagcgtcc tctgcagttt cgacaatggt acagccctga
     2041 gatctggcaa tctcaatgat cttttccgta tccccaaagt tatatgtgca gccgtatgtt
     2101 tccgcataca atctgatact tctcagctgg ttgagtgccg ctgcggtcag agctgttgaa
     2161 tccatatctg ctatacagat ctgtatagaa ctaaataact aagtatctcc acgaaccata
     2221 ctctttcatt tcaggcgtgg catctcatgg gcaaaaaaca caaaaaggaa aaaaagtcca
     2281 ataaggaaaa aaagatctcc agagcagaaa agccattgtg tgaaaactgc ggcagatact
     2341 tcaaccggga actgtcctgg ctgaaattca atgaacgtat cctgacagag gcacgaaatc
     2401 cccaaaaccc actattggag cgggttgctt tccttgggat tgttgccgac aatctggatg
     2461 agtacttcat ggtacgggta ccggcatatc agggaggagc cacccgacca agcgatgagt
     2521 atgaagaggt ggttggcagc cgtacccagc tggagatgat ttatgaccgt accatctttt
     2581 tgatgcgcaa catatcctgg atctgggcaa aggaactcaa accggagctg gagaaaaacg
     2641 gcatactgtt taccaaatat accaagtgca gtgagtctga gaagaagaca ctcagaaaag
     2701 aactatcaga gattcttgcc aagataccgg taaccgttat tcggggagac cggtttgagg
     2761 atatcgaaca caacgagtac ctgaaaggac tgggattact tgccaagtcg gaccgcggct
     2821 ttgccgttgt acctattcag gaaattctgg atacatacgg aagatttgtc cgtgtgggaa
     2881 aacggaaaac ctcatacatg ttccgtgaag aacaactgcg gaaaaactcc gatctctttc
     2941 tcccggatga agcctttcga gctgttgttc ccgttcgtct cacccgtgac tctgacctga
     3001 atctaaaagg agacgatgcc gatgatctga tctcagccat caaagcagca ccggaaacat
     3061 tggcaaaaaa acttccctcg cggctcgaga cagaacagtg gcttccattt gggtatacaa
     3121 gttatctggt gaatgccctg tctctcctcc cggaactggt ctatgatgta ccctcacccc
     3181 tcggacttgc tgatctgaaa aaattcccag tctcacgccc agaactgaag tttgaaccct
     3241 acacaccagc gctcccaacc ggactttctg aagtgaacag gatttttgaa catcttgccg
     3301 acagagacta tatgctcttt accccatatg acagctttga cggacttgtc aatttcctga
     3361 acgctgctgc aaccgatccc gcagtaaaaa caatccaaat gacactgtac cgcctcggtc
     3421 ctgagtcccc ggttgctgat gcactgattt cagctgccaa aaacggaaaa gatgtcaccg
     3481 cagtcattga actgaaagcg agtttcgacg aagaggcaaa cttccagtgg gcaacaaaac
     3541 tgagtgaggg aggggtcact gtaatccatg gtgttcccgg cctgaaagta catgcaaaat
     3601 gctgccttgt tacccgtgca gagggaaata aaacagtcag atatgcaacc atttcaaccg
     3661 gtaactacaa cgcaaaaaca gcaaagatct actcagacat ctcgttattt acagcagatc
     3721 caggaatatg ccatgatgtt gccacactgt tttcatatct taacggaaat gaagaacatc
     3781 cggagtacaa acatctgttg gtcagcccgg atttcatgga agattcccta aaacacctca
     3841 tacggcgtga gatcgaacat cagaaacgat caggaaacgg gcatattgtt cttaagatca
     3901 actccttaac tcaccgaccg attatcaacg cattgtatga ggcatcgaac gaaggagtaa
     3961 agatagatct gatcgtccgc ggtatctgta tgctccgccc aggtgtccca aacctttccg
     4021 aaaacatccg agtcatctca atcgtcggca ggttcctgga acacagcaga gtgttttatt
     4081 tcagaaacaa tggagacgag caggtattca ttggtagccc agatatgatg tcacgtaatc
     4141 tcaagcggcg tgttgagatc atctgtccgg ttctggatcg caaaatcaag aatgcaatca
     4201 tcaatagaat tctccctgcc ttcatccacg acacagcaaa aggacatctg cttacatcag
     4261 atggtatata tctccctccc aagcatcccg aatctaatct gggagcacaa cagatgttta
     4321 tcacaatgag aaaggtttgg tggtagatct gaacaaacag gagttacaga ctatccatga
     4381 tgcagcggtt gtactcaccg aggatttcca tcacgcagaa caggtctgtc gtctctctct
     4441 tgcaatctat gatgatctct catccgctca tggatacggc aagaggacgc gaagacttct
     4501 tgcagcagcg gctcttctgc atgatatcgg ctggagtatc tcaactgtct ctcacaacaa
     4561 gtgtgggatg acattcatta tcgaggatcg gatgctgccg ttatcactgt atgaacggat
     4621 tcttgtggca cttgccgtcc gttatcacag aggagggaca cccaaacgtc gtcacaatct
     4681 ctacggcaat ctctccaaac atgaccggca aaaagtgtgt ctgattgcag ggatcatacg
     4741 gatagcagac gggctggatc gatcccaccg cagtgttgtc cgatcggttc acgcaaaact
     4801 atcagatgaa accctcgtcc tcctctgtac cggatacgga cgaggaggac ctgagagacg
     4861 agcttcagca agaaaagcgg atctgctaat gaaggcatta tcagcaaaga ttcagatctg
     4921 ctggaaacga tgcccataac ctgaattacc ttttccagaa tccggatttc ttttgtgcat
     4981 cctgatttct gaagtactgt tccagtgatg caatctgatc ccagagtgtg tgtacctc
//
LOCUS       DALTZZ010000031         2111 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_4690,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000031 DALTZZ010000000
VERSION     DALTZZ010000031.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4690"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            60..866
                     /locus_tag="O0X12_00530"
     CDS             60..866
                     /locus_tag="O0X12_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HJK52679.1"
                     /translation="MKEKDRTLLLAADELARTVDALAIISFLNKDTEINLNTPVVWIR
                     DIQPEILRDRTMVTLVDYCSNHIIDAVLQYKILTKMQSGTVVAVFPYALLIYDIEDTN
                     SQFNIEQYAGIADLDVLHSVLSLALEIAREGREGRAIGTAFIIGDIEELKRWSHQGVL
                     NPYEGHPESVRDVRIKENWESVKEFAQLDGVFLIDKNGIIGASGRYLDADGRDVHLQS
                     GLGGRHLATAAITKVAPAIGITVSESGGVVRVFVAGTAVAQIRTDIRLSF"
     gene            complement(927..1508)
                     /gene="hisB"
                     /locus_tag="O0X12_00535"
     CDS             complement(927..1508)
                     /gene="hisB"
                     /locus_tag="O0X12_00535"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HJK52680.1"
                     /translation="MRSAEVVRETKETNIRIVFNPDLRGEISIDTGVAFFDHMLNSFA
                     RHGGFSLTVLAKGDLEVDPHHTIEDVGIVLGSAIKQAVGDGRGIQRFAHAIIPMDESR
                     ATVALDVGGRGYLVMDGAFTGIVTGGIPNDLFEHFLYSLCINAGITTHVIFSGVNDHH
                     KCEAIFKAFGVALGEALSLVPGRDDVPSTKGTL"
     gene            complement(1514..>2111)
                     /gene="hisA"
                     /locus_tag="O0X12_00540"
     CDS             complement(1514..>2111)
                     /gene="hisA"
                     /locus_tag="O0X12_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HJK52681.1"
                     /translation="WINAGAKNLHVVNLDGAFSTSSENVKVIREVVAETDVFVQVGGG
                     IRSIADARGWLDAGVDRIIVSTFATKEPKALTALADEFGSERIMAGVDARAGEIAVSG
                     WQETAGDYIEWAQLFEQLGAGSLLYTNVDVEGLQAGIAQEPVKELIEAVSIPVVVAGG
                     VSSVADVSALRRLGAAGCVLGSALYSGKIHLNEALKEA"
BASE COUNT          520 a          578 c          485 g          528 t
ORIGIN      
        1 ctcggtattc cggttgcaac ccgcccgtca gaaattccgc tcctggtgaa ggaggtcata
       61 tgaaggaaaa agatcgtaca ctgcttctcg ctgccgacga gcttgccaga acagtggacg
      121 ctcttgcaat catctctttc ctaaacaaag atactgaaat caatctaaac acgccggttg
      181 tctggattcg tgacattcaa ccagaaattc ttcgtgatcg tacgatggta acgctggttg
      241 attactgttc caatcacatc attgacgcag tattacaata caagattctg acaaaaatgc
      301 agagcggaac tgttgttgca gtgttcccgt acgctttgct gatctatgat attgaagaca
      361 cgaacagtca gttcaacatt gagcagtacg ctggcatcgc tgatcttgat gtcttacact
      421 cggtactctc tcttgcgctt gaaattgccc gcgagggtcg cgaaggacgt gccattggaa
      481 ccgcgttcat catcggcgac attgaggaac tcaaacggtg gtcccatcag ggtgtcttaa
      541 atccttacga gggtcacccc gaatcagtcc gcgatgtgcg gataaaagag aattgggaga
      601 gtgttaaaga gttcgcccag cttgacggag tgttcctgat cgacaagaat ggtatcatcg
      661 gcgcttccgg cagatacctg gatgccgacg gtcgtgatgt ccatctccaa agtggtcttg
      721 gaggccgtca tctggctaca gcagctatca caaaggttgc acccgcgatt ggaattactg
      781 tctctgaaag tggcggtgtt gtccgtgtgt ttgtggcggg aactgccgtc gcacagatcc
      841 ggacagacat ccgtctctct ttctgatctt ttttatgcta ctcaaaagag tataatgttt
      901 tttgggagag atccctgtta aaaaagttag agtgttcctt ttgtgcttgg gacatcatca
      961 cgtccgggca cgagtgaaag tgcctcgccc agcgccactc cgaatgcctt gaagattgct
     1021 tcacatttgt ggtgatcgtt tactcctgaa aagatcacat gcgtggtaat tcctgcatta
     1081 atgcagagac tgtagaggaa gtgctcaaag agatcattgg ggataccgcc ggttacaatt
     1141 ccggtgaatg caccgtccat caccagatat ccgcgtccgc ctacatcaag tgcaacagtt
     1201 gcccgtgact catccatcgg aattatggca tgggcaaacc gctggatacc acgtccgtcg
     1261 ccaactgcct gtttgattgc tgatccgagc acaattccca catcttcaat ggtgtgatgg
     1321 ggatcaacct ccagatcacc ttttgccagt acagtcaggg agaatcctcc gtggcgggca
     1381 aaggagttca gcatatgatc aaagaatgct acacctgtat caatggaaat ttcaccacgc
     1441 agatccggat taaatacaat acgaatgttt gtctctttgg tctcccgcac aacttctgct
     1501 gatctcatga ttctcatgcc tctttcagtg cctcatttag atgaatcttt ccgctgtaca
     1561 atgcagaacc cagcacacaa ccagcggcac cgagacggcg gagggcagat acatctgcaa
     1621 cagagctcac acctcccgca accaccactg ggatggacac tgcctcgatc aattctttca
     1681 ccggttcctg ggcaattcct gcctgcagac cttccacatc cacattcgtg tacagaagcg
     1741 atcccgcacc aagctgttca aacaactgcg cccactcgat gtagtctccg gctgtctctt
     1801 gccacccgga caccgcaatc tcaccggcac gggcatccac ccctgccatg atccgctcac
     1861 tgccaaactc atctgcaaga gcagtcaaag cctttggctc tttggttgca aatgtgctca
     1921 caatgattcg atcaacacct gcatccagcc acccgcgtgc gtctgcaata ctgcggatac
     1981 cgccgccaac ctgtacaaac acatctgtct ccgcaacaac ctctcggatt actttcacat
     2041 tctccgacga tgtcgaaaat gctccgtcaa gattcaccac atggaggttt ttggcaccgg
     2101 cattgatcca c
//
LOCUS       DALTZZ010000032         2031 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_4894,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000032 DALTZZ010000000
VERSION     DALTZZ010000032.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4894"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..99)
                     /locus_tag="O0X12_00545"
     CDS             complement(<1..99)
                     /locus_tag="O0X12_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HJK52682.1"
                     /translation="MYSEKVMDHFTNPRNVGVIEDANGVGEEGNPSC"
     gene            complement(100..1251)
                     /gene="nifS"
                     /locus_tag="O0X12_00550"
     CDS             complement(100..1251)
                     /gene="nifS"
                     /locus_tag="O0X12_00550"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HJK52683.1"
                     /translation="MDHAATTYAAPEVVEAMLPYFSEKFGNPSSVYGIGQENKAAVEE
                     ARAKVAAAINAEPNEIYFTAGGTESDNWVLKGVAFANIRKGKHIIPTAVEHHAILHAA
                     EWLQSQGFEVTYLPVDQYGMVSPADVEKAIRPDTILISVMYANNEVGPIQPIAEIGAI
                     ARAHGIYFHTDAVQAVGHVPIDVKAEHIDMLSLSGHKFYGPKGIGILYIRRGVRIQNL
                     LHGGAQESRHRAGTENVAGIVGLGAAIERSVAAMPEESARLTAMRDHMIRELLKIPAS
                     HLNGHPTQRLPNNVNITFEYIEGEGILLLLNMFGICASTGSACNSASLEPSHVLTAMG
                     VPHEIAHGSVRLTLGERNTEEDVSYVLEKLPEVVRKLRSMSPLTPEELR"
     gene            1423..>2031
                     /locus_tag="O0X12_00555"
     CDS             1423..>2031
                     /locus_tag="O0X12_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJK52684.1"
                     /translation="MYAKRLDNLPPYLFAQIDAIKAQKRAEGVDLIDLGVGDPDLPTP
                     AHIVDALCEAARDPATHHYPDYLGMIEYRKAVAAWYKNRFGVTLDTAREVLALIGSKE
                     GIAHIPEAFVNPGDYVLASDPGYPVYKTSTLFAEGKCHLMPLLEENNFLPDYDAIPKD
                     VVAGAKLMFIGYPNNPTGAVAPMKFFDETVEFAKNNDIIVVHD"
BASE COUNT          459 a          596 c          518 g          458 t
ORIGIN      
        1 gcaggagggg tttccctcct cgccgacacc gtttgcatcc tcaataacgc ccacgttccg
       61 cgggttggtg aagtgatcca tcactttttc gctgtacatt tatctcagct cctcaggagt
      121 caggggtgac atggaccgga gttttctgac aacctccggc agtttttcaa gaacatacga
      181 tacatcctcc tccgtgttcc gctctcccag cgtaagacgg accgagccgt gggcaatctc
      241 atgcggaacg cccattgctg tcagtacatg ggatggttca agggacgcgg agttgcaggc
      301 actgccggtg gaggcacaga tgccgaacat gttgaggagc agaagaattc cctctccttc
      361 aatgtactca aacgtaatgt tcacgttgtt cggcagacgc tgtgtcggat gtccgttcag
      421 gtgacttgcc ggaatcttca aaagttcgcg gatcatgtga tcccgcatcg cggtaagacg
      481 tgctgactcc tcaggcattg ccgcaacgga tcgttcgatt gcagcaccga ggccgacgat
      541 tcccgcaaca ttctcggttc cggcccggtg tctgctctcc tgcgcaccgc cgtgcagcag
      601 attctgaatc cgtaccccgc ggcggatgta gagaatgccg atgcctttcg ggccgtagaa
      661 cttgtgaccg gacagagaga gcatgtcgat gtgttctgct ttgacgtcga tcgggacatg
      721 accgaccgcc tgcaccgcgt cggtatggaa gtagatgccg tgtgcacgtg cgattgctcc
      781 gatctctgcg atcggctgga tgggtccgac ctcgttgttt gcatacatga cggatatgag
      841 aatcgtatca ggccggattg ccttttccac atccgcaggc gagaccatgc cgtactgatc
      901 gacaggcaga tacgtcacct caaatccctg cgactgaagc cactcggcgg catgaagaat
      961 ggcgtggtgc tcaacggcgg tggggatgat gtgttttcct ttgcggatgt tggcaaaggc
     1021 aacgcccttc aggacccagt tgtccgactc ggtgccgcct gcggtgaagt agatctcatt
     1081 cggctctgcg ttgatggcgg ctgcaacctt tgcacgcgct tcttcaaccg cagccttgtt
     1141 ctcctggccg ataccgtaca ccgaagacgg gttgccgaac ttctcggaga agtacggaag
     1201 catcgcctcc accacctccg gggccgcata ggttgttgcg gcgtgatcca tatacacaat
     1261 ttttttctcc gtaccggtca cctcaacaaa ttcacaatgt gaacaaatca gatagaagta
     1321 tattgcactc cttgtatttg aaaagatatg gatatgcgga tagtgacaat tttccctgca
     1381 atccgtgact cttaactatg tcagagtcta acatactaag cgatgtacgc aaaacgcctg
     1441 gacaacttac caccctacct gtttgcccag atcgacgcca tcaaagcaca gaaacgtgcc
     1501 gaaggcgtag acctcattga tctcggagta ggagatcctg atctcccgac tcctgcacac
     1561 attgtagatg cactctgcga agcggcccgt gacccggcaa cccatcacta ccctgactat
     1621 ctcggcatga tcgagtaccg caaagccgtc gcagcatggt acaaaaaccg gttcggcgtc
     1681 accctcgata ccgccagaga agtcctggca ctcatcggat ccaaggaagg tatcgcacac
     1741 atccccgagg cattcgtaaa ccccggagac tatgtactcg catccgaccc gggatacccg
     1801 gtttacaaaa cctcgaccct ctttgccgag ggaaaatgcc acctcatgcc gcttcttgag
     1861 gagaacaact tcctccccga ctacgacgca atccccaaag acgttgtcgc cggtgcaaaa
     1921 ctcatgttca tcggctaccc gaacaacccg accggtgcag tcgcccccat gaagttcttt
     1981 gatgagaccg tcgagtttgc caaaaacaac gacatcatcg tcgtccacga c
//
LOCUS       DALTZZ010000033         2698 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_5194,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000033 DALTZZ010000000
VERSION     DALTZZ010000033.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5194"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            14..1276
                     /locus_tag="O0X12_00560"
     CDS             14..1276
                     /locus_tag="O0X12_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HJK52685.1"
                     /translation="MVIFVILLILSAFFSASEVALVGITRAKVRILSEEGGAAGKRLE
                     KLKNNPDHFLITILVGNNIANVGASAIATAVAFGIFGDAGVAIATGVVTLLLLIFGEI
                     GPKTFATRKTESVALFVATPIYYLTLLLSPFFWVYDRLRRTGKNGTADVPAVTEEEIR
                     EWIDVGEMEGAIEQDEKEMIYSVLRFNDTTAREIMTPRPDVAVIEDTASLEDAVAHFR
                     DTGYSRVPVFHEHTDNIVGTLNIKDLFNAYTAGNHRAFVKTLMFDVYCVPESKKIDDL
                     LRELQLRRMHMAIVLDEFGGFSGVVTFEDILEELVGDIMDESDTDEVADIIEIGEGLY
                     LVDAQARVTLLNEHFGIALPEDPSNYETVGGLVFSRLGHIPRLGESITIAEQYITIVV
                     TKMRGRQILKLKLILPQQETDEQDISGE"
     gene            1504..2151
                     /gene="psmB"
                     /locus_tag="O0X12_00565"
     CDS             1504..2151
                     /gene="psmB"
                     /locus_tag="O0X12_00565"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeal proteasome endopeptidase complex
                     subunit beta"
                     /protein_id="HJK52686.1"
                     /translation="MSQEYQDVKTGTTTVGIVFQGGVVLATERRATMGTLIASKKAKK
                     VHSITDRIGMTIAGGVGDAQQLVRIINVECNLYQIRRGKEISVGAAATILSNYLNQNR
                     FSPYYVQLLVGGVDANGSSIYSVDAAGGATLEEDFVSTGSGSLTAYGVLEDRFKPNMN
                     ETEAIDLAVRALRAAMRRDAASGEGYNVVVITRDKFEYVPETKIAELLQAPAPSL"
     gene            2180..>2698
                     /locus_tag="O0X12_00570"
     CDS             2180..>2698
                     /locus_tag="O0X12_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HJK52687.1"
                     /translation="MQVEDRLKELKDTINRKVPRGVTVTDVEYEGPEMVIYTDDPKRF
                     AEEQDLIRTLARDLRKRIVVRPNILGDTEQAYDMIKSVVPESAGITDIFFDTDTGEVL
                     IEAEKPGVVIGKNGSTLRDITMKVGWTPKVVRTPPIPSVTIKQVRQFLRETRDERKEF
                     LRKCGRRIHRDSM"
BASE COUNT          650 a          642 c          720 g          686 t
ORIGIN      
        1 agaatactca gatattgtta tttttgtaat cctgctgatc ttgtccgcct ttttttcggc
       61 atctgaggtt gcgcttgtcg ggattacccg tgcgaaggtt cgcattcttt ccgaagaagg
      121 gggagctgca ggaaaacgtc ttgaaaaact gaagaataat ccggatcatt tcctgattac
      181 aattcttgtc ggcaataata tcgccaacgt tggtgcttca gcaattgcta ccgcggtagc
      241 gtttggtatc ttcggggatg ctggtgttgc tattgcaacg ggtgtggtaa cactgttgct
      301 gttaatcttc ggcgaaatcg gcccaaagac ctttgcaaca cggaagaccg agtctgtcgc
      361 attgtttgtt gcaacgccga tctattatct gactctcctg ctttccccat tcttctgggt
      421 gtatgaccgt ctccggcgta ctggtaaaaa tggtactgcc gatgttcccg ctgtaactga
      481 ggaggaaatc cgcgagtgga ttgatgtagg tgagatggaa ggggcgattg aacaggatga
      541 gaaagagatg atctactctg tcctccggtt taatgataca actgcacggg agatcatgac
      601 accccgtcct gacgttgcgg tgattgagga tactgcatct cttgaggatg cggttgccca
      661 tttccgcgat accggttact ctcgcgtccc ggtctttcac gagcacactg acaacattgt
      721 cggaacactc aacatcaaag atctgttcaa tgcctacacc gcagggaatc atcgtgcgtt
      781 tgtaaagacg ctgatgtttg atgtctactg cgtaccggaa tccaaaaaga tcgatgacct
      841 tctgcgtgag ttacaactgc gccgtatgca catggcaatt gtcctggatg agttcggagg
      901 attttccggt gttgtgacgt ttgaagacat ccttgaggaa ctggtcggcg atatcatgga
      961 cgaatcggat actgatgagg ttgccgatat cattgagatc ggcgagggtc tctacctggt
     1021 ggatgctcag gcacgcgtta ctctgctcaa tgagcatttt gggatcgcgc ttccggaaga
     1081 tcccagcaat tatgaaacgg ttggtggtct tgttttttca cggctgggcc acatcccccg
     1141 cctcggtgag tcgattacga ttgccgagca gtatattacc attgtcgtca caaagatgcg
     1201 tgggcgtcag attctcaaac taaaacttat ccttccccaa caggaaaccg acgagcagga
     1261 catcagcggg gagtgaaccc ttcttcgttt ttttcgtatc acggtcggca actttatctc
     1321 atcccatgca cattacataa tagcgctttt cactgctgca gatatgcatg cggtcacgac
     1381 cccgggtgtg gggagtggag cagggttata ccttgcggct ttactcaaaa attctcaagc
     1441 gttacctgca aatagcagcc ctttgaggga acagcagaac aggtgtccaa gtacgaggtc
     1501 tccatgtcac aggaatatca ggacgttaaa acgggaacta ccactgtcgg tattgttttc
     1561 cagggcggtg ttgtccttgc gaccgaacgg cgtgccacta tgggtaccct catcgccagc
     1621 aaaaaggcaa agaaagtaca cagcatcacg gatcgcatcg gtatgactat tgccggaggg
     1681 gtcggcgatg cccagcagct ggttcgaatc atcaatgttg agtgcaacct ctaccagatc
     1741 cgccgcggaa aggagatcag tgttggtgca gctgcaacca ttctgtccaa ctatctgaac
     1801 cagaacaggt tctcaccgta ctatgtccag ctcctcgttg gaggtgttga cgcaaacggc
     1861 tcctccattt actcggtgga tgctgccggt ggtgcaacgc ttgaggagga ctttgtttca
     1921 accggttccg gatctcttac cgcctatggt gttctggagg atcggtttaa gccgaatatg
     1981 aatgaaactg aagccattga tcttgcagtc agagcacttc gtgcagcaat gcggcgcgat
     2041 gcagcatccg gcgaaggata caatgtggtg gtcatcaccc gggacaaatt tgagtatgtt
     2101 ccggagacaa aaatagcaga gctgcttcag gcacccgcgc catcactctg atttttacac
     2161 ttttttggaa acgatttcta tgcaggttga agacagactc aaagaactga aagatactat
     2221 aaaccggaaa gtcccccgcg gcgttaccgt aactgatgtt gagtacgaag gcccggagat
     2281 ggtaatatac accgatgatc caaaacggtt tgccgaagag caggatctga tccgcactct
     2341 tgcacgtgat ctgcgcaaga gaattgtggt gcgcccgaac attcttggcg atacggaaca
     2401 ggcgtacgat atgatcaaga gtgttgttcc ggagagtgca ggaattactg atattttctt
     2461 cgacactgat accggtgagg ttctgataga ggcggagaag ccaggtgttg tcatcggcaa
     2521 aaacggatcg acgcttcgtg acattacgat gaaggttggc tggacaccga aggtcgtacg
     2581 aaccccgccg attccctctg tgacaatcaa gcaggtgcgt cagttcctgc gcgaaacccg
     2641 cgacgagcga aaggagttcc tgcgcaagtg cggacgccgt attcaccgtg actcgatg
//
LOCUS       DALTZZ010000034         1835 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_5319,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000034 DALTZZ010000000
VERSION     DALTZZ010000034.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5319"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="O0X12_00575"
     CDS             <1..502
                     /locus_tag="O0X12_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HJK52688.1"
                     /translation="LGMVHSPIILFGCGVCDGQTLRVTQYLLRDIGEEIAALELVADM
                     IRTHPALVTYNGRSFDLPFTNSRLSYYGERECRPALHFDLLHTSRRLFRSDLPDCCLG
                     TVERQILGFGRTEDLPGYLVPVYYQRYLRTGDTSPLKQIVDHNRSDVTTLALLLAKQT
                     EMMYGS"
     gene            492..>1835
                     /locus_tag="O0X12_00580"
     CDS             492..>1835
                     /locus_tag="O0X12_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJK52689.1"
                     /translation="MDPEMYTRVFPARDAVYKEPETLPSPVICTYLKSRDIRLYTHQA
                     DSYDAVMRGANIILTTPTASGKTLAYTLPVLEKLMQNRDATALFIYPTKALARDQLGV
                     LQELDMDLVAKTRPAIYDGDTPRDARAKIRSSSRIILTNMYELHQILAWRMKWGDFWA
                     NLSFVVIDEAHRYRGIFGSHIALLLRRLRRICNYYDAHPQFILSSATIGDAPSFAETL
                     TGVAAVEIANDGSPRAQQTFRLYNPWCSGKSSLSATTDLIQDQVRSGMQTLCFTKSRN
                     MAEITALRCRESLPGSSISSYRGGYRPNERRVIENDLKKGDIAGVISTNALELGIDVG
                     GLDSVIICGFPGTMMSVRQQAGRAGRGGKDALITFIANLNPLDQYFMRCPDAFFDAPH
                     ERPILDLENPYLLREHILCAAAELPYRTERDGEYFGPLAAEIVRSLKEEHLLASTP"
BASE COUNT          410 a          490 c          459 g          476 t
ORIGIN      
        1 gcttggtatg gtgcactcgc ctatcatcct tttcggatgc ggcgtatgtg atggacaaac
       61 ccttcgggtt actcagtatc tgcttcgcga cattggcgag gagatcgcag cactcgaact
      121 cgtcgcggat atgatacgta cccatccagc tcttgtcacc tacaacggga gatcgtttga
      181 tctgccgttt accaacagtc gcctctcgta ttatggagag cgggagtgcc ggcctgcatt
      241 gcactttgat ctgttacaca cgtcaagacg cctgttccgt tcggatctgc cggactgctg
      301 tcttggaacg gtggaaagac agatcctcgg gtttggccgt actgaggacc ttccaggtta
      361 tcttgttcct gtctattatc agaggtatct gcgtactggt gatacctctc ctctcaagca
      421 gatcgttgat cacaaccgga gcgatgttac cacccttgcc ctgttacttg caaaacaaac
      481 ggagatgatg tatggatcct gagatgtaca cacgcgtgtt cccggccagg gatgcagtat
      541 acaaagagcc ggaaacgtta ccgtctccgg tgatttgcac atatttgaaa agccgcgata
      601 ttcgtctcta cactcatcag gcagactcct atgatgcggt catgcggggt gcaaatataa
      661 tcctcaccac tccgaccgcc tcaggaaaaa ctcttgcata cactctgccg gttctggaaa
      721 aactgatgca gaaccgtgat gctactgcgt tattcatcta tcctaccaag gctctggccc
      781 gtgatcagct tggtgttctg caagaactcg acatggatct tgttgccaaa acccgccctg
      841 caatctacga cggtgacact ccccgcgatg ccagggcaaa aatccgcagc agttcccgga
      901 ttattctcac caacatgtat gaactgcatc agattcttgc atggagaatg aagtggggtg
      961 acttctgggc gaatctctcc tttgtcgtta tcgacgaggc acatcggtac cgggggatat
     1021 ttggttcgca tattgccctt cttttacgga ggctgcggcg catctgtaat tattatgacg
     1081 cccacccgca gtttattctc tcctccgcaa ccatcggtga tgccccgtcg tttgccgaaa
     1141 ctctcactgg tgttgcagct gttgagattg caaacgatgg ctctccccgg gcacaacaga
     1201 cttttcggtt gtacaatccg tggtgttcgg gaaaaagcag cctttctgca actactgatc
     1261 tcatccagga tcaggtccgg agcggtatgc agactctctg ctttaccaaa tccagaaaca
     1321 tggcagagat cacagcactt cgctgccgtg agagtctgcc aggcagcagc atctcctctt
     1381 accggggagg ctatcgtcca aacgaacgga gagtcattga gaatgatctc aaaaagggag
     1441 atattgctgg cgttatcagt accaatgccc tggaactcgg tattgatgtt ggcggtcttg
     1501 actcggtgat catctgcggc tttccgggaa ccatgatgtc tgttcgtcag caggcgggac
     1561 gggctggacg cggaggaaaa gatgccctta tcacctttat tgcaaatctc aacccgctgg
     1621 atcagtactt catgcggtgt cctgatgctt tctttgacgc accgcatgag cgcccaattc
     1681 ttgatcttga aaatccctat cttttacggg agcatattct ctgtgccgct gccgaattac
     1741 cctatcggac ggaacgggat ggtgagtact tcggaccact tgccgctgag attgtccgta
     1801 gtctgaaaga ggagcatctt cttgcaagca caccc
//
LOCUS       DALTZZ010000035         3048 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_5324,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000035 DALTZZ010000000
VERSION     DALTZZ010000035.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5324"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..113)
                     /locus_tag="O0X12_00585"
     CDS             complement(<1..113)
                     /locus_tag="O0X12_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52690.1"
                     /translation="MLAVQKSSLHGTVAAPPSKSHTHRAFILAALAKGESVV"
     gene            complement(113..961)
                     /locus_tag="O0X12_00590"
     CDS             complement(113..961)
                     /locus_tag="O0X12_00590"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR01920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HJK52691.1"
                     /translation="MIGRGVSYGALSVINAIAMGKGAGFGIDLKTEATVRLTAEPEIV
                     VEIDGHPSEDTRLARFSVEEMLRRYPESGMQGAVISTTSNIPISQGLKSSSAATNAIL
                     AATADALGVSLDPLEIGRIGATVAIAAGVSITGTFDDACACMLGGLVFTDNPKRELLH
                     RMPMPDGYKAVIHLPEYQVRKSAFPKDRMREMASEVSAAYDRALAGDVFSAMYLNGAC
                     TCRALGIEPEVADRALACGAAAAGLSGTGPATGILVEADRLDEFLDAFGRDQVIVANV
                     RNGDAS"
     gene            complement(958..2379)
                     /gene="aroE"
                     /locus_tag="O0X12_00595"
     CDS             complement(958..2379)
                     /gene="aroE"
                     /locus_tag="O0X12_00595"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HJK52692.1"
                     /translation="MTLICAVIAADTPAAAEAMKVEALSAGAEAFEVRLDAFAEIPDD
                     FSFLPTEKPVIATLRSELDEERREIFAKALASGAAYVDIESDSVLRGVFPKSQVICSY
                     HDFTKTPAAWDILQIFRDLSSSGIPKAAFMVRGPADLLEIWKAAVVLKQSGDPFILIG
                     MGAAGEVTRIRAADIGSMVSYCAVRPEFASAPGQITVAEAARLGDDPIITAITGWPLE
                     HTRSPQMHNAAFRAAGIQGRYVRIPSPAAELPLLPEVLRCYRIRGANVTIPHKQTVMP
                     LLSEVLPEAKSAGAVNTILVDPSGRLTGTNTDIAGISATIAELRITPSNARVLVAGAG
                     GAARAAVSCLTAAGAAVSVTNRTMEKARTLAAEFGATAVPRDELSAGYDLIINATPAG
                     MSGFSQDIPVPVSILTPDTAVFDMVYEPEMTPLLAAAGDAGVRAAVCGKAMLIAQAAA
                     SFTLWTGIEADAAAMTAAFGGSP"
     gene            complement(2376..>3048)
                     /locus_tag="O0X12_00600"
     CDS             complement(2376..>3048)
                     /locus_tag="O0X12_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase II"
                     /protein_id="HJK52693.1"
                     /translation="VTTPEEAKLAFATMEVGADGVVIAPKDPADIAGFSKIKSDEFPD
                     AELTAVPVTKIVPLSLGDRVCIDTCSLLAQGEGMLVGSQSSCLFLVCSESFESEYVNS
                     RPFRVNAGAVHSYILCPDGTTRYLSEIVSGDAVLTRMPDGTLRSVSVGRVKIEIRPML
                     LIEVEVNGRTHSVVLQNAETIRVGTPSGAVSVADLSVGDEVFVRLESGGRHFGHAMAE
                     TICEK"
BASE COUNT          700 a          868 c          869 g          611 t
ORIGIN      
        1 acaaccgact caccctttgc aagggccgca aggatgaagg ctcggtgggt atggctcttg
       61 gagggcggag ctgcaaccgt tccatgcagg gaggatttct gtacggcaag catcatgatg
      121 catcaccatt cctgacattg gcaacaatca cctgatcacg tccgaatgcg tccaggaact
      181 cgtcgagccg gtcggcctcc accagaatgc cggttgcagg accggttccg gaaagaccgg
      241 ctgccgcagc accgcaggca agagcacggt cggctacctc cggttcgatg ccgagtgccc
      301 ggcaggtgca tgcaccgttg aggtacatcg cggaaaacac atcgcccgca agagcacggt
      361 cgtatgctgc ggagacttcg gacgccatct cccgcatgcg gtctttgggg aatgcggact
      421 tgcggacctg atactccggc aggtgaatga cggccttgta tccgtcgggc atcggcatcc
      481 ggtgcagaag ttcacgtttc gggttgtcgg taaagacaag accaccgagc atacaggcac
      541 aggcatcgtc gaatgttccg gtgatgctga cccccgcagc gatggcaacg gttgcaccga
      601 tgcgtccaat ctcaagcgga tcaagggata cgccaagtgc gtccgcggtt gcggcaagga
      661 ttgcgttggt tgccgcactg ctgcttttga gaccctgcga gatcggaatg ttggaggtgg
      721 tggagattac ggcaccctgc attcctgatt cgggataacg tctgagcatc tcttcaacag
      781 agaagcgggc aagccgtgtg tcctctgacg gatgaccgtc tatctcgaca acaatctccg
      841 gttcggccgt gagacggacg gttgcctctg tcttcagatc aataccgaat cccgcaccct
      901 tccccatggc aattgcattg atgacggaaa gagcaccgta tgatacaccg cggccaatca
      961 tggactacct ccaaatgcag cggtcattgc agcagcatct gcctcaatac cagtccagag
     1021 ggtaaaggat gctgcagcct gtgcaataag catcgccttt ccgcagacag ccgcacggac
     1081 acctgcatct cctgctgcgg caagaagcgg agtcatctcc ggttcgtaca ccatatcaaa
     1141 gaccgcggta tccggtgtga gaatcgatac aggaaccggg atatcctgtg aaaatccgct
     1201 cattccggca ggagtggcat tgattatgag atcatagcct gcagaaagtt catcacgcgg
     1261 cactgcggtc gcaccaaact ctgcagcaag agtccgggct ttttccatcg tccggttcgt
     1321 gacagaaacg gctgcacccg ccgcagtgag acaggagacc gccgcacgtg cagcaccgcc
     1381 tgctccggca acaagcaccc gggcattgga cggagtaatc cgcagttcgg caatggttgc
     1441 ggaaattcct gcaatatcgg tgtttgttcc ggtgaggcgg ccggaaggat cgacgagaat
     1501 ggtgttgacc gcaccggcgc tcttggcttc gggaagcacc tcagacagca gcggcatgac
     1561 cgtctgtttg tgcggaatgg tgacgtttgc accgcgtatc cggtaacagc ggagtacttc
     1621 aggaagcagc ggaagctccg ctgcggggga gggaattcta acatagcggc cctgtattcc
     1681 tgcggcacgg aatgctgcat tgtgcatctg cggcgaacgg gtatgttcca gcggccagcc
     1741 ggtgatcgcg gtaatgatcg gatcgtctcc gagtcttgca gcttcagcga ccgtaatctg
     1801 tcccggagcg gaagcaaact ccggccggac cgcacagtaa ctgaccattg atccaatgtc
     1861 tgcggcacgg atacgggtaa cctcgcctgc tgcgcccata ccaatcagaa tgaacggatc
     1921 cccgctctgt ttcaacacga ctgcggcctt ccagatctcc agcagatcgg caggtccccg
     1981 caccatgaac gcggcttttg ggatgcccga ggaggaaaga tcgcggaaga tctgtaggat
     2041 atcccatgcg gcaggtgttt tggtaaaatc atgatacgag cagataacct gagatttggg
     2101 aaatacacct cgcagaaccg agtctgactc aatgtcaaca tatgcggcac cactggcaag
     2161 agctttggca aagatctcac gccgttcctc atccagttcg gaacgaagtg ttgcaatcac
     2221 cggcttttcg gttggtaaaa aggaaaagtc atcagggatc tctgcaaacg catcgagcct
     2281 gacctcaaat gcctcggcac ctgcggaaag tgcctccact ttcattgcct cagcggcagc
     2341 gggggtatct gcggcaatca ccgcacagat cagggtcatt tctcacagat ggtctccgcc
     2401 atagcatgtc cgaagtggcg gcctccgctt tcgagtctga caaacacctc atcgccaaca
     2461 gaaagatctg caactgaaac agcaccagac ggtgttccga cacggatcgt ctcagcgttc
     2521 tgtaagacca ccgagtgtgt tctgccattg acctcaacct cgatcagcag catgggccgg
     2581 atctcgatct ttacccgtcc gaccgagacg gaacgaagcg taccgtccgg catccgcgta
     2641 agcactgcgt cacctgacac gatctcagag aggtaacggg tggttccgtc gggacagaga
     2701 atataggaat gaaccgcccc tgcgtttacg cggaagggcc gggagttgac gtactcgctt
     2761 tcaaaacttt ccgagcagac aaggaacagg catgaagact gtgagccgac aagcattcct
     2821 tctccctgcg caaggaggga acaggtgtcg atacagacac ggtcgccaag cgacagcggc
     2881 acaatcttcg taaccggcac tgcggtgagt tctgcgtccg ggaactcatc gcttttgatt
     2941 ttggaaaacc cagcgatgtc ggcaggatct ttgggtgcaa taaccacacc gtccgcgcct
     3001 acctccattg tggcaaacgc aagtttcgcc tcttcagggg tggtgacg
//
LOCUS       DALTZZ010000036         1964 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_5488,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000036 DALTZZ010000000
VERSION     DALTZZ010000036.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5488"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /gene="hypD"
                     /locus_tag="O0X12_00605"
     CDS             <1..481
                     /gene="hypD"
                     /locus_tag="O0X12_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HJK52694.1"
                     /translation="GYHEYEAFPVPQVVAGFEPEDILLSLLMIARQVENGEAKVENAY
                     PRVVTREGNVKAQRLMQEVFTPTDVEWRGFPVIPDSGLALKPEFEAFDAQKKFDLVYA
                     KVTKNSGCICDRVLRGLADPSDCKLYGKACTPRVPVGPCMVSHEGACRIWYQYQLQK"
     gene            529..600
                     /locus_tag="O0X12_00610"
     tRNA            529..600
                     /locus_tag="O0X12_00610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:562..564,aa:Arg,seq:ccg)
     gene            complement(616..>1964)
                     /locus_tag="O0X12_00615"
     CDS             complement(616..>1964)
                     /locus_tag="O0X12_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HJK52695.1"
                     /translation="FIDSLDGSIVNIALPTIAEGFSVDMSTAAWVIMAYFLFLVGLIP
                     LFGKIADHGKLQEVFCAGFVVFTIGSVVCGFAPDLLMLVAARAVQGVGASMIAVVAPL
                     LVVRLMPPRHWGMGMGMAATAGAVALVFGPVLGGILTEYLSWHWCFFINVPVGIVAVV
                     LGLRIIPKAELQPKMRFDLAGSVLIFTAMASLIYLLERGEPLGWTNPQVIVCGAVFVL
                     SAVLFVIRELRVAEPILRVRVFRSWPFTFVVSSYFLISVVYAGFLYIIPFYMSIVLGM
                     SPAVSGFVLLISSVITAVMGLPSGALSDRIGARWLVTAAGVVRVLFCCVMAVMMPEHG
                     LFWIVLLMVLSGLTFGISGGPSSARIIEHAPEGEGGTGSVVMMLSQYSGMVVGVALYA
                     LVFNLAVPGSAGVAVGLLTMDAFLPGFHMTGVFGAVCSAVVVILSVVVKDKIAKKN"
BASE COUNT          586 a          578 c          448 g          352 t
ORIGIN      
        1 ggggtatcat gagtatgagg cgttccctgt tccgcaggtg gttgcaggat ttgagccgga
       61 ggatattctg ctttccctcc tgatgattgc aaggcaggtg gagaacggtg aggcaaaagt
      121 ggagaatgcg tatccgcggg ttgtgacccg tgaaggaaat gtaaaagcac agaggctgat
      181 gcaggaggtc ttcacgccga cggatgtgga gtggcgcggt tttccggtta ttccggactc
      241 tggccttgca ctgaagccag agtttgaggc gtttgatgca cagaagaagt ttgatctggt
      301 gtatgcgaag gtgacgaaaa actccggctg tatctgtgat cgtgtgctgc ggggacttgc
      361 tgatccgtca gactgtaagt tatacggtaa agcctgtact ccgcgtgttc cggttggtcc
      421 gtgtatggtt agtcatgaag gggcatgccg gatctggtat cagtaccagc tgcaaaaata
      481 atcctcactc tttttatact gaaaagggta atagaataaa gcacatttgc acttgtaggg
      541 tagcggatat cctggaagcc tccggagctt tcgacccggg ttcaaatccc ggcaggtgcg
      601 ctgtatttac ttttctcagt tctttttcgc aatcttatcc tttaccacta cggaaaggat
      661 caccacaacc gccgagcaga ccgcaccaaa cacaccggtc atgtggaaac ccggcagaaa
      721 cgcatccatc gtcaaaagac caacggccac gccggccgac ccgggaaccg cgagattaaa
      781 cacgagggca tacaaagcaa cgcccaccac cattccgctg tactgtgaaa gcatcattac
      841 aaccgaccca gtaccaccct ctccttcagg agcatgttca ataattcggg cactggaagg
      901 accgcccgaa ataccaaacg tcagacccga cagcaccatc agaagaacaa tccagaataa
      961 cccgtgttcc ggcatcatca cagccataac gcagcagaac aaaacccgca caacacccgc
     1021 agcagtcact agccagcggg caccaatccg atccgacaac gcaccggaag gaagccccat
     1081 caccgctgta atcaccgaac ttatcaaaag aacaaatcct gaaaccgcag gagacattcc
     1141 cagaacaatc gacatataaa acggaataat atacaaaaat cccgcataca ccacactaat
     1201 caaaaagtac gacgacacca caaacgtaaa tggccaggac cgaaacaccc tgacccgcag
     1261 aatcggctct gcaacacgga gctcccgaat aacaaacagc acagccgaca aaacaaacac
     1321 cgcaccgcaa acaatcacct gcggattcgt ccagccaagc ggctcaccgc gttccagaag
     1381 atagatcagc gaggccatcg ccgtaaagat cagcaccgaa cccgcaagat caaacctcat
     1441 ttttggctgg agttccgctt tgggaataat ccgaagacca agaacgaccg ccacaatacc
     1501 taccgggaca ttgataaaga aacaccagtg ccatgacaga tattcagtca gtatcccgcc
     1561 aagaaccggc ccgaacacca gcgcaaccgc accggccgtt gccgccatcc ccatacccat
     1621 tccccagtgc cggggaggca tcagccgtac aaccagaagc ggggccacaa ccgcaatcat
     1681 cgatgcaccc actccctgta ctgcccgggc agctaccagc atcagcagat ccggtgcaaa
     1741 accacagaca acggatccga tcgtaaacac cacaaaccct gcacaaaaca cctcctgcaa
     1801 cttcccgtgg tccgcaattt ttccaaacag cggaatcaga cccacaagaa acaaaaaata
     1861 ggccataatc acccatgccg ccgttgacat atccacggaa aatccttccg caatcgtcgg
     1921 cagggcaata ttcacaatcg atccgtccag cgaatcaata aaaa
//
LOCUS       DALTZZ010000037         3858 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_5520,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000037 DALTZZ010000000
VERSION     DALTZZ010000037.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5520"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            95..1006
                     /locus_tag="O0X12_00620"
     CDS             95..1006
                     /locus_tag="O0X12_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HJK52696.1"
                     /translation="MGRTACVVHLTHNDLDAAGSDAVCRMMFGTEILTLFSSVNRFGW
                     FVGQVGGCNGKGDRLIISDLGYQKGIEDQIRKAHAAGWKIEWYDHHKWTEEEMERVRP
                     FVISLTVDTSVCATGVIYRAFAQGNSSASEVARVVCDYDLWKHEDPRSAVLGMVTSKR
                     EYLELVRNKLTEGIIIDDEISQIFADIERDKNACMQKSIRHAKIFRGKYTIAVMPAYG
                     YPSETAAEARKELGCDMELLVFDTGKFSLRSVAPVSHLIAKQFNGGGHPNASGGSFAY
                     GWKEKWMLKLFGRVSCAKEFAKTAELV"
     gene            complement(1096..2778)
                     /locus_tag="O0X12_00625"
     CDS             complement(1096..2778)
                     /locus_tag="O0X12_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52697.1"
                     /translation="MGLEQINEFFINNLVLLIVVIYMAAMIAIGYFVQRKGADKSAEN
                     YLIANKGVGPLMVGGTVFSTNWCGGVLLGAAGTAYAGYVVSTIADPWATCLTLVLMAI
                     FFCAILRKLKIASLSEMYRLRFGKRGATVATVMCIPSMITWTAANIVALTTIFKLFLD
                     FDPLICAIIAGLIVVLYTYLGGMLAVVYTDNIQAVMIMLGLIILIPTGIAFVGGLDVL
                     TAATPENFWNLLPNDGAGAVSSGFTVDPLGIFTWLAGLSGMGFGYLASVELTQRVLCA
                     RDTKAARQGLLIGSGMYALAGFFSMMIALIAIVMVSQGTTTPAGVPMGEILAEDGNYV
                     LLVLAERLFDPSVMGIIGVVLMAIFIGSLLAAIMSTSSSTIFAASAALSTLMLHGSLS
                     KYATTSLQVLRLTRMLVLVIGVFCVFFSFVIDSLYWMMIFSFTILFNCMFWALVGGLF
                     WKRCNASAAIASIVVGFAGVILCIIAQSLYFGQIAIEPQDNLWIGIQTFVPCAMGGIA
                     MLITALLTQKSDPPVALCDTDGELVKWEDLPLSADTIEIRRRIEEIKAREEE"
     gene            complement(3095..3712)
                     /locus_tag="O0X12_00630"
     CDS             complement(3095..3712)
                     /locus_tag="O0X12_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52698.1"
                     /translation="MACCFVFSAGCINTTPSGPIAAGDNVTVEFTMYIGDTVMVTSNE
                     TKYNQILTSGQMPVPLILTEDPVVTAGSTTDNDDLTIPTKTHSNPYWFLIGEYNVIAG
                     NIVGLEKGDTIRIPIYAEGTEKTGFWTNEQCDEQGLEKQLLKAGDRLTYTRTIGDATS
                     TEDGGYDQNNLTNQQTMIHQLLVIDVTNEGVTYQELYDTVEITIV"
BASE COUNT         1078 a          884 c          878 g         1018 t
ORIGIN      
        1 atcttatttt gtcaaagata caacatagta gccaatgcct ttctgtctgc gtaaaaaatc
       61 cgctgaaaag aaaccgagtc tgcaggaaaa gattgtggga agaacagcat gcgtggtaca
      121 tcttacccac aacgatctgg acgctgcagg aagcgacgcc gtttgccgga tgatgtttgg
      181 cacagagatt cttaccctgt tctcgtcggt aaaccggttt ggctggtttg tcgggcaggt
      241 cgggggatgt aacggaaaag gggatcgtct gatcatttcg gatcttgggt atcagaaagg
      301 aattgaggat cagatccgca aggcgcacgc ggcaggatgg aagattgaat ggtatgatca
      361 tcacaaatgg acggaagagg agatggaacg cgtccggccg tttgtgatct ctcttaccgt
      421 ggatacatct gtctgtgcca ccggtgttat ttatcgtgca tttgcccagg gaaattcttc
      481 ggcatctgag gttgcgcggg ttgtgtgtga ttatgatctg tggaaacatg aggatccgcg
      541 ttccgcagtt ttgggaatgg tgacctcaaa gagagagtac cttgagctgg tccggaataa
      601 gttgacagag gggattatca ttgatgatga gatctctcaa atttttgcgg atatcgaacg
      661 ggataagaac gcatgtatgc aaaagagcat ccggcatgca aagatattcc gaggaaagta
      721 cactattgca gtgatgcctg cctatggata tccgagtgaa actgcagcag aagcgagaaa
      781 agaactcggg tgtgatatgg agttactggt atttgatacc ggcaagttct ctctccgaag
      841 cgttgcacct gtgagccatc tgattgccaa acagtttaac gggggtggac acccaaatgc
      901 atcgggtggg agttttgcgt acgggtggaa agagaagtgg atgctgaaac tgttcggacg
      961 tgtctcctgt gcaaaggagt ttgccaagac tgctgagtta gtataatttt ttcaggaaaa
     1021 tgcagtgtaa tctgcaccat tcctttttta gtacattttt agggttgttg caaaaaagaa
     1081 atccaaaaag gagatttact cctcttctcg tgctttaatc tcctcgatac gtctgcgaat
     1141 ctcaatcgta tccgctgaaa gcggaagatc ttcccatttt accagttctc catccgtatc
     1201 acacaatgcc acaggaggat ctgatttttg tgtaaggagt gccgtaatca acattgcaat
     1261 tccacccatt gcacatggaa caaaggtctg gataccaatc cacaggttgt cctgcggctc
     1321 gattgcaatc tggccgaagt agagcgactg ggcaataata caaagaatta cacccgcaaa
     1381 tcccacaaca attgatgcaa ttgcagctga tgcattacac cgtttccaga acagaccgcc
     1441 aacgagtgcc cagaacatgc agttgaacag gatcgtaaac gaaaagatca tcatccagta
     1501 cagactgtct atcacaaagc tgaagaatac acagaatact cctatcacca gcaccagcat
     1561 acgcgtcagc cgaagtacct gtaatgaggt ggttgcatac ttcgacagtg aaccatgcag
     1621 catcagcgtc gaaagagctg cggatgcggc aaagattgtc gagcttgatg ttgacataat
     1681 tgctgcaaga agcgatccaa tgaagattgc cattagtaca acgccaatga ttcccatcac
     1741 acttggatca aacaaccgtt ctgcaagtac cagcagtaca taatttccat cctctgcaag
     1801 aatctccccc atgggaactc ctgcaggagt tgtcgtaccc tgtgaaacca tcacaattgc
     1861 gatcagtgca atcatcatcg agaagaaccc tgcaagtgcg tacatgcctg atccgatgag
     1921 caggccctga cgtgcagcct tcgtatcacg ggcacacaga accctttgtg tcagttcaac
     1981 cgatgcaaga tatccgaatc ccatgccgga aagtcctgca agccaggtaa agattccaag
     2041 cggatctacg gtaaatcctg atgacaccgc ccctgccccg tcatttggaa gaaggttcca
     2101 gaagttctcc ggtgttgctg cagttagtac atcaagtcct ccgacaaatg caattccggt
     2161 tggaatcaga atgatgagac caagcataat cattactgcc tgaatattat ccgtatatac
     2221 aaccgcaagc attccgccca gatatgtgta cagcacaaca atcagaccgg cgataattgc
     2281 acagatcaga ggatcaaaat ctaagaacag cttgaaaatc gttgtcagag caacaatgtt
     2341 tgccgcagtc cacgtgatca tcgatgggat acacatcact gttgcaactg ttgctccgcg
     2401 ttttccgaat ctcagccggt acatctctga aagagatgca atcttcaatt tgcgcagaat
     2461 cgcacagaag aaaattgcca tcagtacaag ggtcagacat gttgcccatg gatcagcaat
     2521 cgtggacacc acatatcccg cgtatgctgt accggcagcg cccagcagta ctccgccaca
     2581 ccagttcgtg gagaaaactg tgccgccaac catgagaggc cctacgccct tatttgcaat
     2641 caggtaattt tctgcacttt tatccgcacc cttacgctga acaaagtatc ctatggcgat
     2701 cattgccgcc atgtagatca caacaatcag cagaacaaga ttgttgatga agaactcgtt
     2761 tatttgctct aaacccatag actacacacc tatatctata gagggaggaa atttacagga
     2821 atactcactg tccgatagac tcgtgataac acacgtatta cgttatggta gttgctcttt
     2881 ttgtttctta aatatgtccc tcctaagacc ttggaagggg tgagtattgg gatgattgtt
     2941 gtagtttcac tcaggatgtc cctgtatcaa actcagtgga ctgtgtctga aatccacatc
     3001 cctgacccca caaagaattt atgggaataa ctcatttgtc aaaaagagat ctcttgcggc
     3061 aagtaaaatt ccctgccgcg cacaactgta ttacttagac aattgtaatt tcaaccgtat
     3121 catagagttc ctgataggta actccttcat tagtcacgtc aataaccagg agctgatgaa
     3181 tcatcgtctg ctgattcgtg aggttgttct gatcatatcc gccatcttca gtgctagttg
     3241 catctccgat tgttcgcgta tatgtcagac gatctcctgc ttttagcagt tgtttctcga
     3301 gtccctgttc atcacactgt tcgtttgtcc agaatcctgt tttttcagtt ccttctgcat
     3361 agattggaat acggattgta tcaccttttt cgaggccaac aatgtttcct gcaattacat
     3421 tgtattcacc aatcaggaac cagtaagggt ttgagtgggt ttttgtaggg atagtaagat
     3481 catcgttatc agtggtagac cccgcagtaa caaccgggtc ttcggttaaa ataagtggaa
     3541 ccggcatctg tcctgatgtt aaaatctgat tatactttgt ctcgttcgac gtcaccatta
     3601 cggtatcgcc gatatacatg gtaaactcaa cggtgacatt atcacctgct gctattggtc
     3661 cagacggtgt tgtattgata catcctgcgg aaaacacaaa acaacatgcc aaaatagcag
     3721 caagtcctgc tgcgagatat ttaatgcaag ttgtatagga ccttatacat taacaccctt
     3781 atgaaacaaa tttcgacgtc tttatttcct tatcttcgcc cgccattctc tggtaaaaaa
     3841 gagtttagtt tattgtgg
//
LOCUS       DALTZZ010000038         1550 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_5630,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000038 DALTZZ010000000
VERSION     DALTZZ010000038.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5630"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..>1550
                     /locus_tag="O0X12_00635"
     CDS             <1..>1550
                     /locus_tag="O0X12_00635"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HJK52699.1"
                     /translation="KKIVCKRCGKENPAKSTYCGGCGELLSSSSATLFCPSCGTKVRA
                     GDTFCGTCGARLSFSQPVERTVGTSKEFGKDRVWIRNPGDFAQRFEIEDIKGFFAKKV
                     TIEQGTKAIFLQGGVYKGFLPAGVYNLGSVLNTISGIKLEERATIILVDAADVPLTFQ
                     LGESELRARNGIAVSASGTVTVYLKYPRDFLENYLKGEKHVSLADLERMIHNELRFVA
                     QQIISRYDASELYGNSVLAGELAAEFQNRLNESVRNRGVEISGLNCVGFDEGAWKEVL
                     AVKQRLSVETEKLAAEYEGKHAVRQMQTQDVSDQLSAEYSIASQKQRQEHDLGTAALA
                     YELERDGMSRGHQRDQDKMDFAQDLAEISALSDLQARKRRQKIEDMDSSSIETRILMN
                     GGTAEDVQKLEQMKRAQSLTPEQILALQTTDPKAAGEALAARAKLASVEELSELRIRD
                     QQAFNQMMQTQYREHAEQMKEVLNTALGAMGQTATARATAQNPGTTVVSGGMGSPVVV
                     QVPSEVQK"
BASE COUNT          368 a          311 c          497 g          374 t
ORIGIN      
        1 ataagaaaat tgtttgtaaa cggtgcggaa aggaaaatcc cgcaaaaagt acctactgcg
       61 gaggctgtgg tgaactcctc agcagttctt ctgcgactct tttctgcccg tcctgcggga
      121 caaaggttcg tgccggcgac acgttttgcg gtacctgcgg tgccagactt tcgttttccc
      181 aaccggttga gcgaactgtc gggacatcaa aggagtttgg caaagaccgt gtatggatca
      241 gaaatcccgg tgattttgct cagaggtttg agatagagga tattaaagga ttttttgcaa
      301 aaaaggtcac tatagagcag ggtacaaagg cgattttttt acagggaggc gtttacaagg
      361 gttttcttcc tgccggtgtg tataatctgg ggtctgtcct taacacgatt tcggggatca
      421 aactggagga gagggctact attattctgg tcgatgcggc ggatgttccc cttacgttcc
      481 agctgggaga gagtgagctc cgggccagaa acgggattgc ggtgagtgct tctggaacgg
      541 ttactgttta tctgaagtat ccgcgggatt tcctggagaa ttatctcaag ggagagaagc
      601 atgtgtcgct ggcagatctt gagcggatga ttcataatga actccggttt gttgcacagc
      661 agatcatctc ccggtatgat gcatcggagc tgtatggaaa ttctgttctt gccggagagc
      721 ttgcagcaga gttccagaac cggttgaatg agagtgtgag aaaccggggt gttgagatct
      781 ccggtctgaa ctgtgttggt tttgatgagg gggcatggaa ggaggtgctt gctgtaaaac
      841 agcgtctttc cgttgaaaca gagaagcttg cagcggagta tgaggggaag catgcagtcc
      901 gtcagatgca gacgcaggat gtttctgatc agctgagtgc ggagtatagt attgcgtcac
      961 agaaacagcg ccaggagcat gatctgggga ctgctgctct ggcgtatgag ctggagcggg
     1021 acggtatgtc ccggggtcat cagcgtgatc aggacaagat ggattttgca caggatcttg
     1081 cagagatttc tgcgctttca gatcttcagg cacgaaagag acgccagaag attgaggata
     1141 tggattccag ttcgattgag acacggattc tcatgaacgg cggtactgcg gaggatgtgc
     1201 aaaaacttga gcagatgaag cgtgcccaga gtcttacacc ggagcagatt ctggcgttgc
     1261 agactacgga tcccaaggct gccggtgagg ctctggctgc gagggcgaag cttgcttcgg
     1321 tggaagagct gagtgaactc aggatccggg atcagcaggc gtttaatcag atgatgcaga
     1381 cccagtatcg tgagcatgcg gagcagatga aggaggtact gaatactgct cttggtgcaa
     1441 tggggcagac ggcaacggcg agggcgactg cccagaatcc cgggacgacg gttgtgtccg
     1501 gcggaatggg tagtccggtt gttgtgcagg tgccttccga ggtacagaag
//
LOCUS       DALTZZ010000039         2396 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_5756,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000039 DALTZZ010000000
VERSION     DALTZZ010000039.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5756"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(143..1501)
                     /locus_tag="O0X12_00640"
     CDS             complement(143..1501)
                     /locus_tag="O0X12_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52700.1"
                     /translation="MFGAGYCLVVRKDCGLEAVMFQSVMFFGLVCGSICVQSRSFSAS
                     RLDAAQYSPTHGTYIIQCGIECCGRFDLSRLASAVFRVATALPLLSSRYVCENGVPVR
                     WEITGDLPEMVTVRYRSLPEEAALPHRMEGRPPLHVIVCRMRDHDDLLVVMDHVATDA
                     RGMFVVLSLLADAYAGREFSVPDQPESRGIEKLFEQVSPDVLRAVAERSRQEVGLYYP
                     WTSPFTRAGAHGSLRIVHRVISSEDFLRIHAAARSFGATVQDILLAAFASALQTLGSS
                     DPVPLESVMDLRPVFGPGASGWVGNFSANYRLTLSCRDGMRETVLQSADFGRSFKANH
                     PEAVCALRFLDSSVRREMDILLPEDVPGLDLRSPFLSNVGVIPSSVGSFGDEITVSAV
                     WIVTDFVSGRYPSLVAGTWNGSLHLSAAVSFADTSVDRIFDQMISSLFSLVDLKEGHI
                     SE"
     gene            1774..>2396
                     /locus_tag="O0X12_00645"
     CDS             1774..>2396
                     /locus_tag="O0X12_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HJK52701.1"
                     /translation="MSFRYNVNDHVPAKEMVTSGLMWTICSAAFVIIFANVVAGLYNA
                     TPAEAVWYAQKLLVITGIAVILQVIFGHRLPAIYGPSAILLTAAVAATDFAPAAFSTA
                     LVLCGAVGIVIARTSLLKRIAKLFTPRVIGTVLTLIPLTLVPTFATLITNPDQPGSMA
                     GKLIFAFLLLILIFACNHRLKGFWRATLMLWVLIFGTVAALICFPGTA"
BASE COUNT          666 a          701 c          564 g          465 t
ORIGIN      
        1 cattaaagaa aagagaacgg aggggactca tgaaggacca aacctcgggt actgtcagag
       61 gtccgcagct ccttggggtg ttacaaaaca gaatacgttt tggggatata tatactcccg
      121 accatgcgta ctgaaagtgc ccttattcag agatgtgccc ctctttcagg tccacaagag
      181 aaaaaagtga agagatcatc tgatcaaaaa tccggtccac agaggtatca gcaaaagaaa
      241 cggctgcgga aagatgcaga ctgccgttcc aggtccccgc aacgagggac ggataacgtc
      301 ctgagacaaa atccgtcacg atccataccg cagaaactgt gatctcatct ccaaacgaac
      361 cgaccgagga cgggatcaca ccaacattgg aaaggaacgg actgcggaga tccagaccag
      421 gcacatcctc cggcagaaga atatccatct ctcgtcggac tgatgaatcc aaaaaccgga
      481 gggcacacac agcctcggga tgattcgcct tgaaggatct gccgaagtcc gcagactgta
      541 atacagtttc ccgcatgccg tctctacagg aaagtgtaag ccggtagttt gccgaaaaat
      601 tcccaaccca tcccgatgcc ccgggaccaa aaacaggacg cagatccatc accgactcaa
      661 gggggacagg atcagaggag cccagtgtct gaagagcgga cgcaaatgct gcgagcagga
      721 tatcctgaac tgtggcacca aaactgcggg cagccgcatg tatccggaga aaatcctcgg
      781 aagagatcac tctgtgaaca atacgaagcg aaccatgggc acctgctctg gtaaacggag
      841 atgtccacgg ataatacaaa ccgacctcct gccgtgatcg ttctgctacg gcccggagaa
      901 catcaggaga aacctgctca aacagttttt caatacctcg gctctcaggc tgatccggca
      961 ctgaaaattc ccttccggca tacgcatccg caagaaggga gagaacaaca aacattcccc
     1021 gggcatctgt ggcaacatga tccataacga cgagcagatc atcatgatcc cgcatgcggc
     1081 agacgatcac atgaagcggt ggtctcccct ccatccggtg cggcagagcc gcctcttcgg
     1141 gaagggatcg atacctcacc gtcaccatct ccggcagatc tcctgtaatc tcccaccgta
     1201 ccggaacccc gttctcacac acataccggg aagagagaag tggaagcgcc gttgctaccc
     1261 gaaacacggc tgatgcaagg cgggaaagat caaacctccc gcagcactca atcccgcact
     1321 gtattatata tgtcccgtgc gtcgggctgt actgggcagc atccaaacgc gaggccgaaa
     1381 atgatctgct ctgcacacaa atacttccgc agaccaaccc aaaaaacatg acactttgaa
     1441 acataacagc ctcaagaccg cagtccttac gcacaaccag acagtaccct gcaccaaaca
     1501 caaccttctg tcaaattcct gcgaaaagag taggggaggc agggactcgt gaaggaccaa
     1561 aaccgcgggt actgtcagag gaccgctgtc tcgaggggtg ttacaaaata aaatacgaca
     1621 tgggggtata taaacatcca accgcgcgta ctgaaaatgc ccccgcaccg gatcaagatc
     1681 aaaacatccg gaaaaatctc cggaaattct cagaaacaaa acctcctgac acgtgccctt
     1741 ataacactcc gcggcaaaaa ctaattcgca tacatgagct tccgctacaa cgtcaatgac
     1801 cacgttcccg caaaggagat ggtaacctcc ggtctcatgt ggaccatctg ctccgcagca
     1861 tttgtcatca tctttgcaaa cgttgtagca gggctttaca acgcaacacc ggcggaggcc
     1921 gtctggtatg cacagaaact tcttgtcatc accggcattg cagtaatact gcaggtaatc
     1981 ttcggtcatc ggctccccgc aatctacgga ccgtccgcca ttctgctgac cgctgcggtc
     2041 gccgcaaccg actttgcacc ggcagcattc tccactgccc ttgtgctctg cggtgcggtt
     2101 ggcatcgtaa tcgcccgcac cagtctccta aagagaattg caaaactctt caccccacgc
     2161 gtaatcggaa ctgtacttac cttaatcccc ttaacgctcg tccccacctt tgcaaccctt
     2221 atcaccaacc cggatcagcc gggaagcatg gcgggaaaac tgatcttcgc ctttttactg
     2281 cttatcctga ttttcgcctg taatcatcgc ctcaaaggat tttggcgggc aacgctgatg
     2341 ctgtgggtgc tcatcttcgg aacggttgca gctctcatct gttttccggg aaccgc
//
LOCUS       DALTZZ010000040         3299 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_5879,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000040 DALTZZ010000000
VERSION     DALTZZ010000040.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5879"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="O0X12_00650"
     CDS             <1..277
                     /locus_tag="O0X12_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HJK52702.1"
                     /translation="YTFTDPYEIALAKHLAQFPYVIEKVVVELRPHLLATYVRDLADL
                     FNSFYRFAPVLKAEGSVRDARLTLVDASRNTLAAALDVLGIEALESM"
     gene            641..1501
                     /locus_tag="O0X12_00655"
     CDS             641..1501
                     /locus_tag="O0X12_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HJK52703.1"
                     /translation="MVTFSPAQVCVKVGSAGKTKCCLPAGNMFVRAFLAGAYIAMGAA
                     LATVGSTGVADVLGAGLAQFILGALFPIGLVLVVLTGAELFTGDAMFAPMAVLQGHTD
                     LKGLIYLWIVVYIGNLIGSLFMAFLVSYGPYTAWDAAGVATATAFGTRAIAIATTKVS
                     YFGPMGILSVFFKGILCNWLVCLALFLGLAADEVVSKILAIWFPIMAFVATGFEHCVA
                     NMYFIPAGIITNMIANGGADTAVLNWGTMWTNNIIWSTLGNIVGAVIFMAVIYYYCYK
                     TEICALCETK"
     gene            1654..2211
                     /locus_tag="O0X12_00660"
     CDS             1654..2211
                     /locus_tag="O0X12_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HJK52704.1"
                     /translation="MKIHGVTIGISPLHILVVVFFLVLFSASYLATSDMMYLLWGIPL
                     SLVLLVIPIFNSYSVGSQYVKLLPEYEELSKRVRIKQINLTMLGKPVRVEGVVQAVKG
                     VWLCRPALTIFDGSAAIVAKKSVPLDADIVVGDNVEVVGMVVRRFTIFGDVMVHAIGI
                     KKVENLTPLEEEPSVPSEEIRIKKY"
     gene            2272..>3299
                     /locus_tag="O0X12_00665"
     CDS             2272..>3299
                     /locus_tag="O0X12_00665"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJK52705.1"
                     /translation="MSHEVIVYSLTGCPHCRNLKGFLDKQHVPYTNYDVGEDEAAANK
                     MIELTGQRGVPVTIIDGQIVIGDDLMKVAMLLDAPSPSDETKDIPKDHELIIIGSGAA
                     GLSAAMYAGRKGLETLVIGGAVGGMATKSSSVENYPGIPDIPGDDLMQKFAQHARDAG
                     AVIIEDVGLSITKDNGLFTVSTLSEKEYTAKAVIAATGRSPRLSGAPGEAEYLGKGIA
                     VCTTCDGPMYKGKTVAILGGGNTAMDMAIEMAGIAAQVHVISATPLDADAVLIGRLTE
                     MKNVTFHTGFTITEFGGGQMLEYVKMLPVDAQPEQKKGFLSSILRQSPEKKLKVDGAF
                     LGVGLDPNT"
BASE COUNT          786 a          770 c          787 g          956 t
ORIGIN      
        1 ctataccttc actgatccct atgaaatcgc gctggcaaaa catcttgccc agttcccata
       61 tgtgattgag aaagttgtcg ttgagttacg cccccacctc cttgcaacct atgtccggga
      121 tcttgctgat ctgttcaact ccttctatcg tttcgcaccg gtgctgaagg ctgaaggttc
      181 tgttcgtgat gcccgtctca ccctggtcga tgccagccgt aatacgcttg ccgcagcact
      241 tgatgtgttg ggtattgaag cactggagtc aatgtagtcg gagatttgtg ttgcctcact
      301 tccttgtgcc tggcagcacg tcctatttca gcctccctct ttttttgtag aaatactttt
      361 tccttggagt atacttctcg attttaccaa ggagaatatc tctacgcgcc tgattttctg
      421 tttcctcaca tcccgcgcag tattttgtat cctcctgtat tcaaacatat tatttacaaa
      481 ttataaatgt aaactaaatt taatttgcca atcgaaaaga gataccaaat aacggaggaa
      541 atttaccggg ttcaatagtt gggttataaa tatgcctccc tccaatttcc aattaacaca
      601 gctctaccgc aaaagcggga gggatgtgag gtgtgtattc atggtaacat tcagcccggc
      661 gcaggtttgc gtcaaagttg gatcggcagg taaaacaaag tgctgcctgc cggcaggcaa
      721 catgtttgtc cgtgccttcc tcgcaggtgc ctacatcgca atgggtgcgg cactcgcaac
      781 ggttggttcg accggtgttg cagatgtact cggtgcaggt cttgctcagt tcattcttgg
      841 agcactgttc ccgattggtt tagttctcgt ggttctcact ggtgcagagc tgttcaccgg
      901 tgacgcaatg ttcgccccaa tggcagtcct tcagggccac accgatctta aaggtctcat
      961 atatctgtgg attgttgtgt atattggtaa cctgattggt tctctcttca tggcattcct
     1021 tgtcagctac ggtccataca ctgcatggga tgcagcaggt gttgcaacgg caaccgcatt
     1081 tggaacccgt gccattgcaa tcgcaactac aaaggtctcc tacttcggtc cgatgggtat
     1141 cctttcggtc ttcttcaagg gtattctttg taactggctt gtgtgccttg ctctgttcct
     1201 cggtcttgca gccgatgaag ttgtttccaa gatccttgca atctggttcc caatcatggc
     1261 tttcgttgcc accggatttg aacactgtgt tgcaaacatg tacttcatcc cggcaggtat
     1321 catcacaaac atgatcgcaa atggtggtgc tgacacggcg gttctgaact ggggaaccat
     1381 gtggacaaac aacatcatct ggtccaccct tggaaacatc gttggtgcag tcatcttcat
     1441 ggcagtcatc tactactact gctacaagac cgagatctgt gcactctgcg aaaccaagta
     1501 atagggcaga ccaagattga aaagcgatta aatcctacgc tttctcaatc tacctctttt
     1561 tgttttcttt tatcatatca aagcatacaa gtatttatgg gggcggaatg catttacagt
     1621 aagagttccg gcaccccggg ggttgtataa tatatgaaaa ttcatggcgt aacaatcggc
     1681 atctctcctc tgcatattct tgtagtggtc ttcttcctgg tactgtttag tgcatcatac
     1741 cttgctacat ctgatatgat gtatctcctg tggggtattc ccttatcact tgtccttctg
     1801 gttatcccta tcttcaacag ctacagtgtt ggatcacagt atgttaagct cttgcctgag
     1861 tatgaggaac tgtccaaacg tgttcggata aaacagatca acctcactat gcttgggaaa
     1921 ccagttcggg ttgaaggggt tgttcaggcg gtcaaaggtg tctggctttg ccgtccggca
     1981 cttactatct tcgatggttc tgcggcaatc gttgcaaaaa aatccgtacc tcttgacgcg
     2041 gacattgtcg ttggagacaa tgttgaggtt gtgggtatgg tggtccgccg ctttaccatc
     2101 tttggtgatg tgatggttca tgcgattggt attaaaaaag tggaaaatct tactccactt
     2161 gaagaggaac catctgttcc ctctgaagag atccgaatta agaaatacta actctctttt
     2221 tttcgccgat aaccgagagt attaatcatc agcaatcgat acaatcagta catgtctcat
     2281 gaagtgattg tttactctct gacagggtgt ccacactgcc ggaatctcaa gggttttctg
     2341 gataaacagc acgttcccta caccaattat gatgtgggag aagacgaagc agctgcaaac
     2401 aaaatgatcg agcttactgg tcagcgtggt gttcccgtaa cgatcataga tggccagatt
     2461 gtcatcggtg acgatctcat gaaggttgca atgcttctgg atgctccctc gccatctgac
     2521 gagacaaaag atattccaaa agatcacgaa ctgatcatta ttggttccgg tgcagccgga
     2581 ctttctgctg cgatgtatgc gggacggaaa ggtcttgaaa cccttgttat cggaggtgcc
     2641 gtaggtggca tggcaactaa gagcagttcg gttgagaact atcctggcat tcctgatatt
     2701 ccgggtgatg atctcatgca gaaattcgcg cagcatgctc gtgatgcagg tgctgtaatc
     2761 attgaggatg tcggtctatc aattactaag gataatggcc tcttcactgt ctctacactt
     2821 tccgaaaaag aatatactgc aaaagcagtg attgccgcaa ccgggagatc cccgcgtctt
     2881 tccggggctc ctggtgaagc agagtacctg ggaaagggga ttgctgtctg taccacctgt
     2941 gatggcccga tgtacaaagg taaaacggtt gcaatccttg gcggaggcaa cactgctatg
     3001 gatatggcaa tcgagatggc aggtattgca gcacaggtgc atgttatctc tgcaactcca
     3061 ctggatgcgg atgccgtcct tatcggccgc ctgactgaaa tgaaaaatgt caccttccat
     3121 accggattta cgatcaccga gtttggtggt ggtcagatgc ttgagtacgt caagatgttg
     3181 ccggttgatg cgcagccaga acaaaagaag ggattccttt ccagtatttt gagacagtct
     3241 cctgagaaga aactcaaagt agatggagcg ttcctcggtg tcggccttga tccaaatac
//
LOCUS       DALTZZ010000041         1991 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_6167,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000041 DALTZZ010000000
VERSION     DALTZZ010000041.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6167"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(53..1237)
                     /locus_tag="O0X12_00670"
     CDS             complement(53..1237)
                     /locus_tag="O0X12_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HJK52706.1"
                     /translation="MAAREICDRIYSVGTIDWDLRYFDAIMPTPRGSSYNAYLVKGSE
                     KTALIDTCKPNDEADFITNLMRLEQHSLDYIVIDHAEQDHSGLLPLLLEVYPSAKIVT
                     NETCKGLLLAMHHLEEVEDRFITIGDRDTLSLGDKTLKFYLAPWVHWPDTMFTEVVED
                     KVLFTTDFLGTHYAAPALYQDDESPDYLEAAKRYYAAIMMPFRSSVQDHLKLLDEIAP
                     KIIGPSHGPVLKMPAKIVDLYRDWSGDNPKNLVIVAYVSMHGSTELMTKFLVDDLVQR
                     GIPVQQYNLLETDTGVLGSSIVDAATIIVATPTVLFGPHPVAVNAAYLVRALRPKVKH
                     LGVIGSYGWGTNAVNYLGEMLAPLGAEMLDPVYIKGLPEEEAIKALHAMADAIEQRHK
                     EL"
     gene            complement(1244..1399)
                     /locus_tag="O0X12_00675"
     CDS             complement(1244..1399)
                     /locus_tag="O0X12_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HJK52707.1"
                     /translation="MDKYRCVICGYVYDPEKGAGIMHPAGTAFEDLPDTWKCPVCGAP
                     KSQFKKV"
     gene            1520..>1991
                     /locus_tag="O0X12_00680"
     CDS             1520..>1991
                     /locus_tag="O0X12_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK52708.1"
                     /translation="MIPKRSDIMEEMSANKKIRDIICGHFLIDIYTPVLALILPLLIA
                     QMNLSYFLAGLIVTVFNVTSSVSQPFVGLYGDKTGWRASVPLCLLIGSVGISLTALSG
                     NYLLLLFLAAGAAVGHALFHPAAMDIMYRLSPPAKRGFYNSIFTTSGSIGYAIGP"
BASE COUNT          469 a          490 c          531 g          501 t
ORIGIN      
        1 atatatatct gaggattggg gtaagaaacc aaaaaaagag agtaaaagga gatcaaagct
       61 ccttatggcg ctgctcgatg gcatcggcca ttgcatggag ggctttgatt gcttcctctt
      121 caggaagacc cttgatgtac accggatcca gcatctcagc accgagcggt gcaagcatct
      181 ctccaagata attgacagcg ttcgtgcccc agccgtaaga gccgataacg ccaagatgct
      241 tcaccttcgg gcgcagtgca cggacaaggt aagcagcatt cactgcaacc ggatgcgggc
      301 cgaaaagaac ggtcggggtt gcaacaataa tcgttgccgc atccacaatg gagctgccga
      361 gaacaccggt atccgtctcc aacagattat actgctgaac cggtatgccc cgctgaacga
      421 gatcatcgac gaggaacttg gtcatcagtt ccgtacttcc gtgcattgag acatatgcca
      481 caatgacaag gtttttcgga ttatctcctg accagtcgcg gtaaagatca acaatctttg
      541 ccggcatctt caggactgga ccgtggctcg gtccgatgat ctttggagca atctcatcca
      601 gaagtttgag gtgatcctgt accgaagaac ggaacggcat catgattgca gcgtaatacc
      661 gctttgccgc ctcaaggtag tcaggacttt catcgtcctg atacagtgcc ggtgcggcat
      721 agtgagtgcc gaggaagtcg gtcgtaaaga gcactttatc ctcaaccact tcggtaaaca
      781 tcgtgtcagg ccagtggacc cagggagcaa gatagaactt cagtgtcttg tctccaagag
      841 aaagcgtgtc ccgatcaccg atggtaatga acctgtcctc aacctcttcg agatgatgca
      901 ttgccagtaa cagacccttg caggtttcgt tggtgacaat ttttgcactc ggatacacct
      961 caagaagcag cggcagaaga ccggaatggt cctgttctgc atgatcgatg acgatgtagt
     1021 caagcgagtg ctgctcgagg cgcatgaggt tggtgatgaa gtctgcttcg tcgttcggtt
     1081 tgcaggtatc aatgagtgcg gtcttttcac tgccttttac gaggtacgca ttgtagctgc
     1141 ttccccgcgg ggtcggcatg atagcatcaa agtaccggag atcccagtca atcgttccga
     1201 cggagtagat acgatcgcat atctcacgcg ctgccattgg tagttacacc tttttgaact
     1261 ggctcttggg tgcgccgcag accgggcact tccaggtgtc agggagatct tcaaaggcgg
     1321 ttcctgccgg atgcatgatg cctgcgcctt tttcggggtc atacacgtat ccacagatta
     1381 cacaacggta tttgtccatt ttgattttcc ttccttgtaa taaggcacat tactattggg
     1441 atcgataatt tatattttgt tgggatgtga catggggggt ggcatttcct gctgcatcac
     1501 cgaacttcct ttataaggga tgataccaaa acgtagtgat attatggagg agatgagtgc
     1561 aaacaaaaag attcgcgaca ttatctgcgg tcattttctg attgacattt acacgccggt
     1621 actagcgctg attcttccgc tgctgattgc acagatgaat ctttcgtact tccttgcggg
     1681 actgatcgtg accgtattca atgtgacctc ctcggtatca cagccgttcg ttggtctgta
     1741 cggggacaaa accggatggc gggcaagcgt gccgctgtgt cttttgatcg gaagtgtggg
     1801 tatcagtctc acagcacttt caggaaacta tctgctgctg ctgttccttg cggcaggggc
     1861 ggcagtcggt catgcgctgt tccacccggc ggcaatggac atcatgtacc gcctgagtcc
     1921 gccggcaaaa cgcgggttct acaactcgat ctttacgacg agcggaagta tcgggtacgc
     1981 gatcggtccg g
//
LOCUS       DALTZZ010000042         2501 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_6222,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000042 DALTZZ010000000
VERSION     DALTZZ010000042.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6222"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(100..321)
                     /locus_tag="O0X12_00685"
     CDS             complement(100..321)
                     /locus_tag="O0X12_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HJK52709.1"
                     /translation="MKITAKEGLVLKNRLKIARAEKHLSQGELAELVGVSRQTISSIE
                     TGQFCPTAKLALILAIALDKKFEDLFYFE"
     gene            complement(327..638)
                     /locus_tag="O0X12_00690"
     CDS             complement(327..638)
                     /locus_tag="O0X12_00690"
                     /inference="COORDINATES: protein motif:HMM:NF039453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6442 family protein"
                     /protein_id="HJK52710.1"
                     /translation="MNKEEILAASRYENRGMDEREQKISFNAQIFAGMIVTLVALLFA
                     ILYFIKGQNLYCPTAIIFTNLAAISIYQYKYMKKKVSLLIGILAVFIVVMNIILFVWK
                     G"
     gene            complement(751..1524)
                     /locus_tag="O0X12_00695"
     CDS             complement(751..1524)
                     /locus_tag="O0X12_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HJK52711.1"
                     /translation="MNQKLTVKDVMSKPVSIAKSALIPEALDKMLAEGVDPIVVTSDR
                     VVVGTASRRSIAEKMGSKKTGNIPASQIRVASVTREDFTSVYQDQDVDLLVPLLQRYK
                     IVVVWDEEHRLIGQVTTGDLLKVAKPEGAIDTMVEIAPRISRDDRVVQLHRRMVGDGA
                     TRFIVMDGNKAVGIVTETDIAKILVKLKGEIDKHFENALRNVTAADIMSSPLITTPAN
                     TPVSKVVDLMLSKNISTIPIAEGDKVIGLATRKSLIDAL"
     gene            complement(1690..>2501)
                     /locus_tag="O0X12_00700"
     CDS             complement(1690..>2501)
                     /locus_tag="O0X12_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HJK52712.1"
                     /translation="VISVPPTMSIIEGVQMMSRHSFRRLPITDPGNKKLIGIVTITDV
                     INMMGGGSRYNLVANRHKGSLLSALNDDIRAIATERVDSLSPSTSIQEAICLLVKSGH
                     GSFPITNPDNTIAGIVTEYDIMKMLAGTESELIVDEIMTRNPKVVSPDVAINCVTKQM
                     VDHDYRRLPVVSNDLLIGMITATDIMGYLGRGRVFTEMKTGSVDEVLNLPVRDLMTGA
                     NIRTMSPDEKINDVAREMLEFGIGAFPVIDEGKLIGIVTEFDLVKGLAGNV"
BASE COUNT          581 a          621 c          557 g          742 t
ORIGIN      
        1 atctattttt gttgccgggt acatgggatt tgtagggtta ttcagcatag tagtaaaaat
       61 tggtcggcag agtttatacc ggtgccgaat ccggttgcct tactcgaaat agaacagatc
      121 ttcaaatttt ttatcaaggg ctattgccag gatcagggca agttttgcag tggggcagaa
      181 ctgcccggtt tcaatggaac tgatagtctg tcgtgaaaca ccaacgagct ctgcaagctc
      241 tccttgcgag agatgtttct ctgcacgtgc aatttttagt ctgttcttta agacgagtcc
      301 ttcttttgca gtaattttca cggtccttat cctttccaga caaagagaat aatgttcatg
      361 acaacaatga atacagcaag aatcccgatg agcaaggata ctttcttttt catatatttg
      421 tactgataga tggatatagc cgccagattc gtgaatataa ttgctgtagg acagtacaag
      481 ttttgtcctt ttatgaaata cagaattgca aacagaagcg caaccagagt aactatcatc
      541 cctgcaaaaa tctgtgcatt gaaagatatc ttctgttcgc gttcgtccat ccctctgttt
      601 tcgtatctac tggctgcaag tatttcttct ttgttcatgt ttcgtcctct catatgacaa
      661 gtgtacattg cattaattgc caattatact tgtcggtttg acatgtttac ttggcacaga
      721 tgatcttgaa aaaaaggtga atgaatctgt tcaaagcgca tcgatcagcg acttgcgggt
      781 tgcaaggccg atgactttgt caccctctgc gattggaatg gtactgatgt tctttgaaag
      841 catcagatcc actaccttgg ataccggcgt gtttgcaggt gtcgtaatca gcgggcttga
      901 cataatatct gccgctgtta cattgcgcag tgcattttcg aagtgcttgt cgatctctcc
      961 cttgagtttc acgaggattt ttgcaatatc cgtttcggtc acaataccga ctgctttgtt
     1021 tccatccatc acgatgaatc tcgttgcccc gtctcctacc atgcggcggt gaagctggac
     1081 aacacggtca tcccgcgaga ttcttggggc aatctccacc atagtgtcga tggctccttc
     1141 cggctttgca actttaagaa gatcgccggt cgttacctgt ccgatcaacc ggtgctcttc
     1201 atcccataca acaacgattt tgtatctctg gagcagcggg actaacaaat ccacgtcctg
     1261 atcctgataa accgaggtga agtcctcacg ggtcacgctt gccacacgaa tctgtgatgc
     1321 aggaatgttg ccggtctttt tgcttcccat cttctccgca atactacggc gggatgcggt
     1381 accaacgacc actctgtcgc tcgtcacaac aattgggtcc actccttcgg caagcatctt
     1441 gtcaagagcc tcagggatta acgctgattt ggcaattgac actggcttgg acattacatc
     1501 ctttacggtt aatttctggt tcattttgct gcctcctgta tttgaattct ttttgtattc
     1561 aacatagtat gcaaggaaca cttcctccag ctttttcctg tggaatctcc cctgctgtct
     1621 ggatcattct cagcaccggt cagatcatct gctctctttt ttccagagcc gatcggtgca
     1681 atgtttgtat cacacgtttc ccgcgagtcc tttcaccaga tcaaactcgg tcacaatacc
     1741 gatcagtttc ccttcatcaa tcaccgggaa tgcaccgata ccaaactcta gcatctcgcg
     1801 agccacatca ttgatctttt cgtccggtga catggtccgt atgtttgctc ctgtcatgag
     1861 atcccgcacc ggcaggttaa ggacttcgtc cacggacccc gtcttcatct cggtaaacac
     1921 cctgccgcgt ccgaggtatc ccataatatc agttgcggtg atcataccga tcaggagatc
     1981 attggacaca accggcagac ggcggtaatc atgatccacc atctgtttgg tgacgcagtt
     2041 gatcgccaca tccggcgaaa ccaccttcgg gttccgtgtc atgatctcgt caacaatgag
     2101 ttctgactcg gttcccgcga gcattttcat gatatcatac tcggtcacta ttcctgcaat
     2161 ggtgttgtca ggattggtga tcgggaaact tccgtgcccg cttttgacca gaagacagat
     2221 tgcctcctga atcgaggttg acggcgaaag gctgtccact ctctctgtag caattgctcg
     2281 gatatcgtcg tttaatgcag aaaggaggga acccttgtgg cggtttgcaa caagattgta
     2341 acggcttcct cctcccatca tattgattac gtcggtgatg gtgacaatgc cgatgagttt
     2401 tttatttcct ggatcggtga tcgggagacg acggaagctg tgccggctca tcatctgcac
     2461 accttcaatg atactcatcg tcgggggaac ggatatcaca t
//
LOCUS       DALTZZ010000043         1643 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_6426,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000043 DALTZZ010000000
VERSION     DALTZZ010000043.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6426"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="O0X12_00705"
     CDS             complement(<1..716)
                     /locus_tag="O0X12_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2099 family protein"
                     /protein_id="HJK52713.1"
                     /translation="MVDEHVIEAVGMTRVVIRDGTVVETGEPKIRSCPLAKRFANPVD
                     PITSEAVAANISSRIQRFGMCTRDRMVLDKETFVLFGASELMNTGLRTGTLDAAVIAC
                     DGAGTVIVRSPEMVQGIGGRMSGLVSTTPYPEVIARIEAEGGVVVNPETAEMDALAGL
                     AKAKEMGWTKTAVTIAGFQHELAEEIREKYPEALIIVVHTSSVSSLENAMRLARAADL
                     VFSCASKYVREAAASTALVQG"
     gene            776..1507
                     /locus_tag="O0X12_00710"
     CDS             776..1507
                     /locus_tag="O0X12_00710"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HJK52714.1"
                     /translation="MGRYWELDACRGVAILLMIGYHVLFQISFFDPALLPWFDPYVFT
                     GAPIAFLFVTIAGISLILFTAKEASLPKTAQKMFVRGVYILFFAAVITIASWLLFPSE
                     VVVFGILHLIGCSTILAIPFVVLKIRGWIVFGFGVIIIALSPFLAYVRGPALLIPLGI
                     TPVGFATLDYEPLIPWFGVLLIGVALGSVLYRGGVRCKVLEKLGEMPKVASPLAFLGR
                     HSLLIYLIHNPIIFAVLWIAGVIPW"
BASE COUNT          358 a          423 c          382 g          480 t
ORIGIN      
        1 ccctgtacca gtgctgtcga tgcggcagcc tcacgtacat actttgatgc acaggagaag
       61 acaaggtctg ctgcccgcgc cagccgcatt gcattttcaa gcgatgatac cgaggatgta
      121 tgcaccacaa tgatcagtgc ttcaggatat ttctctcgga tctcctcagc aagttcgtgc
      181 tggaacccag caattgttac cgccgttttc gtccacccca tctcttttgc cttggcaagg
      241 cccgcaagag catccatttc ggcggtctct ggattaacca caactccccc ctctgcctca
      301 atgcgtgcaa tgacctctgg gtagggagtg gtggaaacaa ggccggacat cctgccgccg
      361 ataccctgta ccatttcggg tgatcggaca attacagttc ccgcaccatc gcatgcgatt
      421 actgcggcat ccagtgtacc tgtccgaaga cccgtgttca tcagttcgga tgcaccaaac
      481 agtacgaatg tctctttgtc aagaaccatt ctgtcccgtg tacacatacc aaacctctga
      541 attcgggatg agatatttgc agcaactgct tcacttgtaa tgggatccac cggattggcg
      601 aatcgttttg caagcggaca ggatctaatc tttggttcgc ccgtttcaac gactgttccg
      661 tcgcgaatta ctacacgggt cataccgact gcttcaataa catgttcgtc aaccatggga
      721 atcacataac tgatgtatag atatggttcg gcaaaaaaca taagatcact acactatggg
      781 acgatactgg gaactcgatg catgccgcgg tgtggcaatc cttctgatga taggttatca
      841 tgtactgttt cagatcagtt tttttgatcc ggccctgctc ccgtggtttg atccctatgt
      901 tttcactggt gccccgattg catttctgtt tgtgacaatc gctggtatct ctctcattct
      961 ctttacggca aaagaggcca gtcttccgaa gactgcacaa aaaatgtttg ttcgaggtgt
     1021 ctacattctc ttctttgccg cagtgatcac aatcgcctca tggctgctct ttccatctga
     1081 agtggtagtg tttgggattc tgcatctgat cggatgttca acgatccttg ccataccttt
     1141 tgttgtgtta aagatacgtg gatggatagt cttcggcttt ggtgtaatca tcattgccct
     1201 ctcaccattc cttgcctatg ttagaggtcc ggcactgctg attccgttag gcataactcc
     1261 tgtcgggttt gcaacactgg actatgaacc gcttattccc tggtttggag ttctgttaat
     1321 cggtgttgcc ctcggttctg ttctctacag aggtggtgtc cgctgcaagg tactggaaaa
     1381 gcttggtgaa atgccaaagg ttgcctcgcc tcttgcattt cttggacgtc attccctcct
     1441 tatttacctg atccataacc cgatcatctt tgcagttctc tggattgcag gagttatacc
     1501 ctggtaaccg gggtggagtt cattcgctgc agcgatggaa atgtatttat gaatcagcga
     1561 tagaactctc tgtaatatga cggcaggttt ttttgaccga tttaccgcga agaaaccgtg
     1621 gatcgtggag agcgtccctc cac
//
LOCUS       DALTZZ010000044         5684 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_6431,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000044 DALTZZ010000000
VERSION     DALTZZ010000044.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6431"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1289
                     /locus_tag="O0X12_00715"
     CDS             <1..1289
                     /locus_tag="O0X12_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HJK52715.1"
                     /translation="ARQGFAETKTLPAHRRAEILRRVADKIDEERETFVDILIHESGK
                     TVRQAHFEVTRAVSVMRTSAEESVRLTGEMIPLDKTEAGEGYTGYYYKVPSGPVLCIT
                     PFNYPLNLACHKIGPAIAAGCSFVLKPSTKTPLSALLLGKIILEAGYPAKAVNVVPCT
                     GSSAETLVRDPRFSVLSFTGSPAIGWRLKELFPGRYIGLELGGNAAAVVHKDADLTYA
                     ARRIAEGAVSNAGQSCISVQRVYLHADIYDTCLAMILNNMKSFVVGDPRLPETDVGPM
                     ISEADAVRAEEKIKQAVMRGARILAGGVREGALFYPTVLERASSEMSVCSSEMFAPVI
                     TVHQYTDVDEAVRQVNESRYGLQGSIFTNSLDVLERTVTKLEVGAVIINDYPTFREDS
                     MPYGGTKFSGIGREGPKYLIDEFMEKRLVVMRRTSP"
     gene            1286..1552
                     /locus_tag="O0X12_00720"
     CDS             1286..1552
                     /locus_tag="O0X12_00720"
                     /inference="COORDINATES: protein motif:HMM:NF020648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1922 domain-containing protein"
                     /protein_id="HJK52716.1"
                     /translation="MIREGFAVIGCPRCRRMQVADLTHTTKTCICGHKIDLTKVKLLA
                     VSRSADEAGSILRSITSRKNTGFQPAANMYNSTSAEKSTKKDTD"
     gene            complement(1562..1825)
                     /locus_tag="O0X12_00725"
     CDS             complement(1562..1825)
                     /locus_tag="O0X12_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HJK52717.1"
                     /translation="MASPEEMIKQCVMMLQAISEDSTIPRNIRRVADETKTILLDEKK
                     TTGLRAADALSLIDEISNDPNMPVYARTSIWQLVSTLEAVPLD"
     gene            1963..2376
                     /locus_tag="O0X12_00730"
     CDS             1963..2376
                     /locus_tag="O0X12_00730"
                     /inference="COORDINATES: protein motif:HMM:NF018392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52718.1"
                     /translation="MTNGARKPDDIMAEYLLGGAKMLADLCPKCGAPMFEVKGNRMCV
                     VCAEKAATSQTETPKVVPANGAESLPEKVRVITPKYAKPQSTPTPVSSDIPARLDALI
                     LQFCARAENEPDPARCLSYMECIRTAAEARTMLNR"
     gene            2418..2957
                     /locus_tag="O0X12_00735"
     CDS             2418..2957
                     /locus_tag="O0X12_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJK52719.1"
                     /translation="MKVIGVVGYPASGKGEFSQIAAGLGIPVVVMGDIIRRQVSAQGL
                     ALTDENIGATARKLREDFGMDAVAMLTAEEVGKQHTDVVVIDGIRGDAEVRYFRSVFP
                     TFVLVAVEASFPVRLSRMQVRGRSDDTTTAESLKGRDEREESFGLAAAMTLAEVRLVN
                     ESTRDVYEAAVRKVLSEEL"
     gene            2954..3733
                     /locus_tag="O0X12_00740"
     CDS             2954..3733
                     /locus_tag="O0X12_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HJK52720.1"
                     /translation="MKIYFASSRLSLKDPDSWVASIAEAGFDGWEISMDGWFHSVEDI
                     PKKFPGIKKVLKETGLEASVHLPFSGLNPASLNTDVWSATVSQLSACIEAASEIADTI
                     TLHPGYLEPNSREATSAAWNAHREALEKFGEAAERSGVCVALENMPNLEDFYCRDPYE
                     LAGFADAVSGISLTFDVGHANTNGNLDTFCKVILPRAAHMHIHDNHGKYDEHLPLGKG
                     SIDWKRVMPKIRAEYRGKIMVVEGRNPAEGRISLDLIREWF"
     gene            3736..4677
                     /locus_tag="O0X12_00745"
     CDS             3736..4677
                     /locus_tag="O0X12_00745"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HJK52721.1"
                     /translation="MAGGETLFVHFLGTAGALPTPNRNPSCIMIRRGSDTLLFDCGEG
                     AQQQMMRAKTGFTVNAIFVTHWHADHYLGIFGLVETMAFNGRTEPLTIYGPAGVEGFV
                     EIIHRLTPKIPFVLSAVEVADGDVTLFDGYSVMAFKTYHGMESVGYVLEEDMRPGRFN
                     REGAIALGVPPGPLFGKLQRGESVRIVRDGEEVTISPEQVMGERRRGRKVVYTGDTRP
                     VKNKNDLLEGADLLIHEATFDEEEGSARAKEVWHSTAREAGMVAAAVRPKILALVHFS
                     SRYTSTANHIQDAKEFFDGEVVAPADLSVIEIPFSDV"
     gene            complement(4751..>5684)
                     /locus_tag="O0X12_00750"
     CDS             complement(4751..>5684)
                     /locus_tag="O0X12_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52722.1"
                     /translation="GLYGFSKMPDVLALVLLVGGLLLLGIFFWYEMRAANPLIPVQII
                     LKNRRFTFNNSANLLYYVAIYAMGSLVSMYMTHAWNFSALDRALVVTTQGLILVLFTI
                     VTGKFYDHLLPRWLILSGVGMAVVGGAAAYLLGVPSADPIWLLSAVIAASIAAFGCGS
                     IVLAVIDRMVKNAPPRYATTTGLFIIALGMVLLLTCGAEQSVWTMIAVQALFGVGIAI
                     FVTPNSTAIMNAVSEQEYGMASGTLSTTRMLGMAVSIGMMTVLTNLFLGAGVQMGPEY
                     VEEFLVMMHATVLAAITVLVIAMILSWTADSRTS"
BASE COUNT         1509 a         1381 c         1571 g         1223 t
ORIGIN      
        1 gtgcaagaca agggtttgcc gaaacaaaaa ctctgccggc tcaccgccgc gcagaaatcc
       61 tccgaagagt tgcagacaaa atagatgagg aacgcgagac atttgtggat atcctgatcc
      121 acgagtcggg aaaaacggtt cgtcaggcac attttgaggt aacacgtgca gtatcagtga
      181 tgcggacatc cgcggaagag agcgttcggc ttaccggaga gatgatcccg ttggataaaa
      241 cagaggctgg cgaagggtat accggttact actacaaagt accttcagga ccggtattat
      301 gcatcacccc gttcaattat cctctgaatc ttgcctgcca caaaattggt cccgcaatag
      361 ccgcaggatg ctcctttgtt ctcaaacctt ccacaaaaac accactgtca gccctcctgc
      421 tcggcaagat tattctcgaa gcaggatatc ccgcaaaggc ggtcaatgtt gtcccgtgta
      481 cgggatccag tgccgaaacg ctggtacgcg atccacgttt ttcagtactg tcctttaccg
      541 gaagccctgc aattggatgg agactaaaag agttgttccc gggaagatat atcggccttg
      601 agctgggagg aaatgcagcg gcagttgtcc ataaagacgc agacctcaca tatgccgcac
      661 ggagaattgc cgaaggagca gtatccaatg cgggccagag ctgtattagt gtacagcggg
      721 tttatctcca cgccgacatt tacgacacct gtcttgccat gattctgaat aatatgaaat
      781 cctttgttgt gggcgatcca cgtctgccgg aaaccgatgt cggccccatg atatcggaag
      841 ctgatgccgt gagagctgag gagaagataa aacaggcagt aatgcgggga gcacggattc
      901 ttgcaggagg agtacgggaa ggagcgttgt tttatccaac ggttctggag cgcgcatcat
      961 ccgagatgag tgtctgctca agcgagatgt ttgcccctgt gattacagtc caccagtaca
     1021 cagacgtcga cgaggctgtc cggcaggtaa atgaatcaag atacggcctg cagggaagta
     1081 tctttaccaa ctctttggat gtgctggaaa gaactgtcac aaagttagag gtaggggcag
     1141 ttatcatcaa tgattatcca acattccgcg aggactccat gccctacggc ggtacaaaat
     1201 tctctggtat cggacgagaa ggaccaaagt atctgataga cgagtttatg gaaaagcggc
     1261 tggttgtcat gcgccgcacg agcccatgat ccgcgaagga tttgctgtga tcggctgtcc
     1321 ccgctgcaga cggatgcagg ttgccgatct cacacataca acaaaaacct gtatctgtgg
     1381 ccacaaaata gatctgacaa aggtaaaact gcttgcagta tccagatcag cagatgaggc
     1441 aggatctatc ctgagatcca taacctcacg gaaaaacaca ggatttcagc ctgctgcaaa
     1501 tatgtacaac tcaacatctg cggaaaaatc aacaaaaaaa gatactgact gagatgatac
     1561 ttcagtcaag cgggacagct tcaagcgttg acacgagctg ccagatgctg gtccgggcat
     1621 agaccggcat gtttggatcg ttgctgatct catcaattaa ggacagggca tctgctgccc
     1681 gcagtccggt tgtcttcttt tcatcaagaa gaatggtctt tgtctcatct gcgacacggc
     1741 ggatgttgcg gggaatcgtc gagtcctcgc tgattgcctg aagcatcatc acacactgtt
     1801 ttatcatctc ttccggactt gccatcttga aaatccctcc tgaaagcatg acctatccta
     1861 atagaacagg atagcattac ctcaaaacta tgctttagat tagagcgtgc acaaagataa
     1921 aggatgctat ctatatgtga cgaacagact aatagtatta caatgacaaa cggtgcacga
     1981 aaacctgacg atatcatggc cgagtatctt ctcggagggg caaagatgct ggcggatctg
     2041 tgcccaaagt gcggggcacc aatgtttgag gtgaaaggca accgtatgtg tgtggtctgt
     2101 gccgagaaag cagcaacctc acagacagag actccaaaag tggtcccggc aaacggagct
     2161 gagtcactcc ctgagaaggt acgggtgatt acccccaagt atgccaaacc gcagagcaca
     2221 ccaactccgg tttccagtga cattcccgca agactggatg ctctgatact gcagttctgc
     2281 gcccgggctg agaatgaacc ggatccggca cgatgtctgt catatatgga gtgtatcaga
     2341 acagcagcag aggcccggac aatgctgaac cgctgaaagc gatgggctta atgaaattcc
     2401 ctgcatatga attacagatg aaagtaatcg gtgttgtcgg atatccggcg agcggaaagg
     2461 gtgaattttc gcagattgcc gcgggacttg gcattcctgt cgtggtaatg ggagatatta
     2521 tccggcggca ggtaagtgcc cagggccttg ctctcaccga tgagaatatc ggagccactg
     2581 cacgcaaact tcgcgaggat ttcgggatgg atgcggtcgc aatgctgacc gctgaagagg
     2641 ttggaaaaca gcatacggat gtggtggtta ttgacggaat ccggggagat gcagaggtgc
     2701 ggtacttccg gtcagtattc ccgacatttg tgctggtagc agttgaagct tcgtttccgg
     2761 tacggctttc ccgaatgcag gtacgaggga gaagcgatga taccacaaca gcagagagcc
     2821 tgaaaggccg cgatgaacgc gaggagtcct tcggtctcgc agccgcgatg acacttgccg
     2881 aagtacggct cgtgaatgag tcgacacggg atgtgtatga ggcagcggtg cgcaaggtgc
     2941 tttcggagga gttatgaaga tttattttgc ttcatcacgg ctttccctaa aagatccgga
     3001 tagctgggtt gccagtattg ccgaggcggg gtttgacgga tgggagatct ccatggacgg
     3061 atggttccac agtgttgagg atatccccaa gaaatttccg gggatcaaaa aagtcctgaa
     3121 agagacaggg cttgaggcct cggtacatct gccgttttca ggcctgaacc cggcatcact
     3181 aaacacggat gtgtggagcg caacggtgag tcagctctcc gcatgtattg aggccgcatc
     3241 tgagattgca gacaccatta ccctgcatcc gggatacctt gaaccaaaca gccgcgaggc
     3301 gacatcagcc gcatggaatg cccacagaga ggcgctggaa aagtttggag aggcagcaga
     3361 acgttcggga gtctgcgtgg cactggagaa tatgccgaac cttgaggact tttactgccg
     3421 ggacccatat gagctggcag gttttgcgga tgcagtttcc ggaatctccc tgacatttga
     3481 tgttggtcat gcaaacacaa acggaaatct ggacaccttc tgtaaagtga ttctgccgcg
     3541 ggcggcacat atgcacatcc atgacaacca cgggaagtac gatgagcatc tcccgcttgg
     3601 gaaaggatca atcgactgga agagggtcat gccaaagatt cgggcagagt accggggaaa
     3661 gattatggtt gtggaaggga gaaatcctgc cgagggcagg atcagtctag atctgatcag
     3721 ggagtggttc tgagtatggc tggcggagag acgctgtttg tacattttct gggaactgct
     3781 ggtgcgctcc cgacaccaaa caggaatcct tcctgtatta tgatccgccg aggttcagac
     3841 acacttctgt tcgactgtgg ggagggagcc cagcagcaga tgatgcgggc aaagaccggg
     3901 tttacggtaa atgcgatctt tgtaacgcac tggcatgcag atcactatct tggtatattc
     3961 gggctggtgg agaccatggc attcaacggc agaacagagc cgctgactat ctatggacct
     4021 gccggagtgg aggggtttgt ggagatcatt caccggctga caccaaagat tcctttcgta
     4081 ttgtctgcgg tggaagtagc tgacggtgat gtaacactgt ttgacggcta ctctgtgatg
     4141 gcgtttaaga catatcatgg gatggagagt gttgggtatg tgctggagga ggatatgcgt
     4201 cccggacggt ttaaccgcga aggagcaatc gctctcggag ttcctcccgg cccgctgttt
     4261 ggaaaactgc agcgcgggga gagtgtacga atcgtccgtg acggagagga ggtcacaatc
     4321 tctccggaac aggtgatggg cgagcgccgc cgcggcagaa aggtggtgta caccggcgac
     4381 acacggcccg tgaagaacaa aaacgatctt cttgaaggag cggatctgct gatccacgag
     4441 gcaacatttg acgaggagga gggatcagcc cgcgcaaagg aggtctggca ctcaaccgca
     4501 cgggaggctg gaatggttgc cgcagcggtt cgtccgaaga ttctggcact ggtgcatttc
     4561 agttcgcggt acacgtctac ggcaaaccac atacaggatg caaaggaatt ctttgacgga
     4621 gaagttgttg cgccggctga cctgtccgtg attgagattc cgttctcgga tgtctaagca
     4681 gaacttccag tcagcagaaa aaaacatcat actggatacc atatccagaa aatctctttt
     4741 tagaaaaaaa ttaggatgtc cgggagtctg cggtccagga tagaatcatt gcgataacca
     4801 gcaccgtaat cgccgcgaga actgttgcat gcatcatcac cagaaactct tcaacatact
     4861 ccggccccat ctgcacaccg gcaccgagga acagattggt aaggaccgtc atcatcccga
     4921 tcgagactgc catcccaagc atccgcgtcg tcgagagtgt tcccgatgcc atgccgtact
     4981 cctgctcgct caccgcgttc atgatcgcag tactgtttgg agtcacaaag attgcaatac
     5041 ccacaccaaa cagtgcctga accgcaatca tcgtccagac agactgttct gccccgcagg
     5101 tcaggagaag caccatgccg agtgcaataa taaacagacc ggttgtcgtc gcatagcggg
     5161 gaggcgcatt tttcaccatg cggtcaatta ccgcaagcac aattgatccg cagccaaacg
     5221 ctgcgatact tgctgcaatc accgctgaaa gcaaccaaat agggtccgca gacggaacac
     5281 caagcagata tgccgcagca ccgccgacaa ctgccatacc cacaccagac agaatcaacc
     5341 agcgcggcag cagatggtcg tagaacttgc cggtcacgat cgtaaacaga acaagaatca
     5401 ggccctgcgt tgttaccacc agcgcacgat ccagagccga gaagttccag gcgtgggtca
     5461 tatacatcga gaccagggaa cccattgcat aaattgccac ataatacaga agatttgccg
     5521 agttattgaa cgtaaatcta cggttcttca ggataatctg tacgggaata agtgggtttg
     5581 ccgcacgcat ttcgtaccag aaaaagatgc caagcagcag aagacctcca accagcagca
     5641 ccagtgcaag tacgtcaggc atcttggaaa agccgtagag tcca
//
LOCUS       DALTZZ010000045         1583 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_6521,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000045 DALTZZ010000000
VERSION     DALTZZ010000045.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6521"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="O0X12_00755"
     CDS             complement(<1..1314)
                     /locus_tag="O0X12_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJK52723.1"
                     /translation="MKIDSLPLPISLRTKYSALGITELYPPQAECVDAGLFERKNLLV
                     AIPTASGKTLVAEMAMQKEISDGGKCLYIVPLKALAAEKYEDFSGKEIKVGIATGDPD
                     QRDEYLGRYDIIVATSEKTDSLLRNRSEWLKRVSLLVVDEIHLIGDPSRGATLEMVIA
                     KLRYQNPSMQIIGLSATMGNPEELAKWLNAQLITSEWRPVDLREGVYHNGAIHFHGTE
                     RIVPTPKKDDDLNLLLDTVEEGGQCLVFVSSRRSAEAYAKRAAAVLKKKSADLDGYAE
                     RIAKADTTPSGKVLANAVKNGAAFHHAGLPRPAREAVEEGFRKGDIQVIASTPTLAAG
                     LNLPARRVIIRDYQRYEAGVGMSPIPVMEYRQMAGRAGRPRLDPYGEAVLIAKDRGAV
                     DRLFEEFIDAPAENISSQCQKENELRNHLLALIATGFAPTRSEVEG"
BASE COUNT          332 a          430 c          393 g          428 t
ORIGIN      
        1 cccttccact tccgatcgtg tgggtgcaaa tccggttgca atcagtgcca gcagatggtt
       61 gcgaagctcg ttctctttct ggcattggga tgagatgttc tctgctgggg catcgatgaa
      121 ctcctcgaag agtcgatcga ctgcaccgcg gtcttttgcg atcagaactg cttcaccgta
      181 cggatccagt ctcggccgcc cggctcggcc tgccatctgc cggtactcca tgaccggaat
      241 cggactcatt ccgactcctg cctcatatcg ctgatagtcg cggatgatta cccgtctggc
      301 cggaagattc agaccggccg ccagggttgg tgttgaggcg ataacctgaa tatcaccttt
      361 ccggaatccc tcctcaaccg cttcgcgggc gggtcgcggc aaacctgcgt gatggaatgc
      421 cgcaccgttt ttcaccgcat ttgccagaac ttttccggat ggtgttgtgt ccgcctttgc
      481 gattctttct gcatagccgt caagatcagc tgacttcttt ttcagaactg ctgccgcacg
      541 cttcgcgtac gcttcagctg atcgccgcga ggagacaaac actaggcact gcccgccctc
      601 ttcgacggta tccagcagca gattcagatc atcgtccttc tttggtgttg gtacaatccg
      661 ttctgttccg tggaagtgaa tggccccgtt gtgatacacc ccctcccgga gatccacagg
      721 ccgccattca ctggtgatga gctgtgcatt tagccacttc gcaagctctt caggatttcc
      781 catcgttgcg gataggccga tgatctgcat ggagggattc tggtaccgca gttttgcgat
      841 caccatctcc agcgttgctc cccgggaagg atcgcctatg aggtgaatct catccacaac
      901 aaggagcgat acccgcttca gccactcgga tcggttccgc agaagtgaat cagtcttctc
      961 tgaggttgcc acaatgatat cataccggcc caaatactcg tcgcgctgat caggatcccc
     1021 ggttgcgatt ccaactttta tctcttttcc tgaaaagtcc tcatattttt ctgcggcaag
     1081 agcttttagg ggaacaatgt agagacattt tcctccgtct gaaatctcct tctgcatcgc
     1141 catttcggca acaagagttt tgcctgatgc agtgggaatt gcgacaagca ggttctttcg
     1201 ctcaaacaga ccagcatcta cgcattccgc ctgaggtggg taaagttcgg tgattcccag
     1261 agccgaatac ttcgtgcgca gcgaaattgg aagaggcagt gagtctattt tcatggaatg
     1321 gtctatccgg tatatgaacc cctttgtttc gagtggaact ttggagaaac tctcttcgta
     1381 aataaatatt gtgaatgata gcttctcaat agtagtagct tatcacattc atcttggctt
     1441 cctttttctt gcggtcaagg aagttgaaca cagtgttgtg atccgagccg ctgatcagca
     1501 taagaactgc ggtatgggca tcgtttacct ggtccggcaa accaataatg ccgatggtct
     1561 ttccatatac cgagatgtag gtc
//
LOCUS       DALTZZ010000046         2287 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_6631,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000046 DALTZZ010000000
VERSION     DALTZZ010000046.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6631"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="O0X12_00760"
     CDS             <1..141
                     /locus_tag="O0X12_00760"
                     /inference="COORDINATES: protein motif:HMM:NF039241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5814 domain-containing protein"
                     /protein_id="HJK52724.1"
                     /translation="DLFPADILSFLEDSVHLLEAVRSVADLAGQKDLVKLSDEHIRQI
                     AR"
     gene            191..961
                     /gene="cofE"
                     /locus_tag="O0X12_00765"
     CDS             191..961
                     /gene="cofE"
                     /locus_tag="O0X12_00765"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HJK52725.1"
                     /translation="MNPGFSVYGVETGLLEPGDDIVGRVITGLAKTDAGEIHDGDILL
                     FAESPLSTTEGRNIRLADITPGKEARELAKQYSLDPRIAEIVIAESDKILGGIPGFLL
                     AARGNLVLPNAGVDESNAPDGWVTRLPKDANASAARIRKEILNRTGKEVAVIIIDSRT
                     HAMRLGVSGVAIGCSGILPITDERGKPDLFGHELQVTRRAIADSLASTAELLMGEANE
                     GVPVVLVRGYPYIFAENAVIESIAPDEDLFLGSLMKKE"
     gene            complement(1445..1909)
                     /locus_tag="O0X12_00770"
     CDS             complement(1445..1909)
                     /locus_tag="O0X12_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HJK52726.1"
                     /translation="MVEFDLDFQDAIREVEEKGAKATAAEWLARVEAEYGTAPLIYKK
                     LEDRPEALISHLLYKNAVNQMSSLEPRVVELISLAVGAALRCDHCTAYHMQVAKKMGI
                     PKEEILEAVLVAGLLANSSVLANAYRVVIPDAGKQPCGASCSISGIPAKKEE"
     gene            complement(1912..>2287)
                     /locus_tag="O0X12_00775"
     CDS             complement(1912..>2287)
                     /locus_tag="O0X12_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
                     /protein_id="HJK52727.1"
                     /translation="FMHRTQTEADIHRLDLVIEEVERVISEKRKELSHDPLSVSPARI
                     QDMIKQEIPEIEDVYSPSPIAVQLTGLRVKPAYEEYAQRIRDLVIEGDITVGDVADVL
                     PSRMRNYILIRIRPLSETNIVV"
BASE COUNT          613 a          580 c          544 g          550 t
ORIGIN      
        1 gatctcttcc ccgcagatat actcagcttt ttagaagatt ccgttcatct cctggaagca
       61 gtacgcagtg ttgcggatct tgccggacag aaagatctgg tcaaactatc tgacgaacat
      121 atccgccaga tagcccgctg acgcacattc ccatcacgtt tttttgagat acagacacaa
      181 tagatcatac atgaatccgg ggttttccgt ctatggggtt gaaacaggtc ttcttgagcc
      241 gggagacgac attgttggcc gggtgattac aggccttgcg aaaacagatg caggagagat
      301 tcacgacgga gatattctcc tgttcgcaga gtctcccttg tccacaacgg aggggcggaa
      361 catccggttg gccgatatta cacccggaaa agaagccaga gaacttgcaa aacagtacag
      421 tcttgacccg cgaatcgcag agattgtcat tgcagaaagc gataagatcc tcgggggtat
      481 ccccggattc cttcttgctg cacggggaaa tctggttctt ccaaatgccg gggtggatga
      541 gtccaacgca ccggacggct gggttacccg gctgccaaag gatgcaaatg caagtgctgc
      601 acgcatccgg aaagagatcc taaaccgtac aggaaaagag gttgcagtaa tcattataga
      661 ctcgcgtaca catgcaatgc ggctcggtgt ttccggagtt gcaatcggat gttctggtat
      721 tctccccatc actgatgagc ggggaaaacc cgatctcttc ggacatgaat tacaggtaac
      781 acgcagggca attgccgatt cgcttgcatc aacagcagag cttctgatgg gagaggcaaa
      841 tgaaggtgta cctgtagtgc ttgtacgtgg ctatccgtac atttttgcag aaaatgcagt
      901 catcgagtcc attgcacccg atgaagatct tttcctcgga agtctgatga aaaaagagtg
      961 acaaagccac atcttcgcac atgaaagagg tgcaacggat gtgtcactga tctgaaaaaa
     1021 atatttttta gttccgcaat gttccaatca ccactatgct gcagacgatc agtcacgccc
     1081 accagaagat gaacgatgat gactccggct gtaacccccg acagaaacct tatgcaggtt
     1141 tatgagatcg ttttttatcc gcacaagtgt cgccaaggaa agttggattt gccgagccac
     1201 tcatgaggaa cgtcgtcgtc gaaattatgt tcgagttgag aaagataggt ctcgcgtctc
     1261 ctctttactc tcgggatgat gaattgtatc agttctagta cagtactggg ggcagagaat
     1321 catcagacca gaacaaaaac aaacgcaaaa acgattacgt atcccttcaa aaaccggcat
     1381 atcccagaaa gaacactaaa aaattgaaga caggacccta taaaatcctg agtatgatga
     1441 caaattattc ttcttttttg gcaggaattc cggagataga gcagcttgcc ccacacggct
     1501 gcttgccagc atcaggtatc acaacacgat aggcatttgc cagaacggat gagtttgcaa
     1561 gaagtcctgc aaccaggaca gcctccaata tctcctcctt aggaataccc atcttctttg
     1621 caacctgcat gtgatatgcc gtacagtgat cacagcgaag tgctgccccc actgcaaggc
     1681 tgatcaattc aacaactctt ggttccagac tgctcatctg attcacggca ttcttgtaga
     1741 gaagatgtga gatcaacgcc tcggggcggt cctcgagttt tttgtaaatt aacggagcag
     1801 tcccgtactc tgcctcaaca cgagccagcc attctgctgc ggttgctttc gcccctttct
     1861 cctcaacctc gcggatagca tcctggaaat ccaaatcgaa ctcgaccatg attacaccac
     1921 aatgttcgtc tctgaaaggg gacggatcct gatcagaata taattccgca tccgtgacgg
     1981 aagaacatca gcaacatcgc cgaccgtaat gtcgccctca attaccagat cgcggatacg
     2041 ctgtgcatac tcttcgtagg caggttttac ccgcagaccg gttagctgta ccgcaatcgg
     2101 tgacggggag tacacgtcct cgatctccgg gatctcctgc ttgatcatgt cctgaatccg
     2161 tgccggtgat actgacaggg gatcgtggga caactctttt cgtttctcgg agataacacg
     2221 ttccacctcc tcaatcacca gatcaagccg gtgaatatcc gcctcggtct gtgtcctatg
     2281 catgaac
//
LOCUS       DALTZZ010000047         2136 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_6697,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000047 DALTZZ010000000
VERSION     DALTZZ010000047.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6697"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1506
                     /locus_tag="O0X12_00780"
     CDS             <1..1506
                     /locus_tag="O0X12_00780"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HJK52728.1"
                     /translation="EKYYAARKDGNFIVVFAETKDVAEIERILADSGFQSVTIPNETG
                     SVQAAVDKYTADQSQAKAAFDDANAKISELKAKYADLLAACDEVLSGDVERAEAPLRF
                     ATTEEAFVIDGWVPADHIEPITAGLNQATGGRVYVTVDDDEIDATAIPVEYNNPSFAK
                     PTELFMDVYARPRYNEIDPTLILAIMFPIFFGLIVGDVGYGILFLILALFARKLAMFK
                     GEGGKNLIKILVGSSISTIFFGLLYSECLGYSLPWHAIIFQRHLAIGADAAAHASGPD
                     AMPLLIVSVWFGIIYITLGRIFGMINHYRMDHAGKHRTKAILGQFGWIAILWGLLVLV
                     WSFFSMEQMFGIVGMMPDLTTAPMIVPGFSVAALAGLILLVVGCILLAQENVLDLMEI
                     PTIISHVLSFCRIAAVGLSSVAIAMVVNYLSFGMFIDPALANLDAVGVIMIIVGVLIF
                     ICGHALNVGLGILGGGLHSIRLHYVEFFTKFYVGGGIKYNPFGLKRKFSEE"
     gene            1512..1763
                     /locus_tag="O0X12_00785"
     CDS             1512..1763
                     /locus_tag="O0X12_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HJK52729.1"
                     /translation="MTIETMTVEAAQAMASGYTAIGAGLAVGLAGVGTGLGEMGIGAA
                     AVGATAEDRDMFGLALLFTVLPETIVIFGLVISLLLLFQ"
     gene            1784..>2136
                     /locus_tag="O0X12_00790"
     CDS             1784..>2136
                     /locus_tag="O0X12_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HJK52730.1"
                     /translation="MGLEVVVDEIKAKGEREAARIKSEAEAEAKTIVADATKRAEEIR
                     IAAEADADLQAERIMIREVAAANLVVKRDQLNAQKELLDKVYCEAAKEIAELPAEMHA
                     KAVRSLLKDAVKQIK"
BASE COUNT          538 a          556 c          525 g          517 t
ORIGIN      
        1 gaaaaatact atgcggcccg caaagacggc aacttcatcg ttgtattcgc cgaaacaaaa
       61 gatgtcgcag aaatcgaacg catattagcc gactcaggct tccagtccgt tacgattccg
      121 aacgagaccg gtagtgttca ggccgcagtg gacaagtaca ctgccgacca gagccaggca
      181 aaagctgcct ttgatgatgc aaatgcaaaa atttccgagc tcaaagcaaa atacgccgac
      241 ttactcgccg catgcgacga agtactgtcc ggcgatgtgg aacgtgcaga agccccgtta
      301 cggttcgcaa caactgagga agcattcgtt atcgacggat gggttccggc agatcatatc
      361 gaaccgatta ccgcaggctt aaaccaggca accggtggcc gcgtatatgt gactgttgac
      421 gacgatgaga tcgacgcaac agcaattccg gtggagtaca acaacccatc gttcgcaaag
      481 cccactgaac ttttcatgga cgtttatgca cgtccaagat acaacgaaat tgacccgaca
      541 ctgatcctcg ccatcatgtt cccgatcttt ttcggactga ttgttggtga tgtcggatac
      601 ggtatcctgt tccttatcct tgcactcttt gccaggaaac tggcaatgtt caaaggcgaa
      661 ggcgggaaaa accttatcaa aattctcgtc ggctctagca tctccacaat attcttcggc
      721 ctcctgtaca gcgagtgctt aggatattcc ctgccatggc acgctatcat cttccagcgt
      781 cacttagcca tcggagcaga tgccgccgcc cacgcaagcg gaccggatgc aatgccctta
      841 ctgattgtat cagtctggtt tggtatcatc tacatcacgc ttggccgcat ctttggaatg
      901 atcaaccatt acagaatgga tcacgccgga aaacaccgga ccaaagcaat tctcggacag
      961 tttggatgga tcgccatcct ctggggtctc ttagtcctcg tctggtcctt cttctcaatg
     1021 gaacagatgt ttggcattgt cggcatgatg cctgacctga ccactgcacc catgattgtc
     1081 cctggattct cggtagcagc tcttgcagga ctcattctgc tcgtcgtcgg atgtatcctc
     1141 cttgcacagg agaatgtgct cgacttaatg gaaatcccga ctatcatctc gcacgtgctt
     1201 tccttctgcc gtattgctgc agttggactt tcatccgttg caattgcaat ggtggtcaat
     1261 tacctctcgt ttggtatgtt tatcgaccca gcccttgcaa acctggatgc agtcggtgta
     1321 ataatgataa ttgttggtgt tctcatcttc atatgcggac acgctctcaa cgtaggtctt
     1381 ggtatccttg gtggaggtct ccactcgatt cgtcttcact acgttgaatt cttcaccaag
     1441 ttctacgttg gtggaggcat caagtacaat ccgtttggac ttaaacgcaa attctcagag
     1501 gagtaaaaat tatgacaatt gaaacaatga cagttgaagc agcacaggca atggcatccg
     1561 gatacaccgc aattggtgca ggtctcgcag tcggtctcgc cggcgtcggt accggtcttg
     1621 gtgaaatggg tatcggagct gccgcagtcg gagcaaccgc agaagaccgt gacatgttcg
     1681 gtcttgctct tctcttcact gtgcttccgg aaaccatcgt catcttcggt cttgtgattt
     1741 cactgctgct gctcttccag taaagcggag gctcgtgcaa atcatgggac ttgaggttgt
     1801 agttgacgaa atcaaggcaa agggtgaacg cgaggcagcc cggatcaaat ccgaggcaga
     1861 agctgaagca aagaccattg ttgccgatgc aaccaaacgt gcagaagaga tccgcatcgc
     1921 agctgaagcc gatgctgacc tgcaggcaga acgcatcatg atccgtgaag ttgctgccgc
     1981 aaacctggtc gtaaaacgcg atcagctcaa tgcacaaaag gaactgctcg acaaagtcta
     2041 ttgcgaagct gcaaaagaaa tcgcagaact tccggccgag atgcacgcaa aggccgtacg
     2101 ttcccttctg aaggacgcag taaagcagat caagga
//
LOCUS       DALTZZ010000048         6977 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_6747,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000048 DALTZZ010000000
VERSION     DALTZZ010000048.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6747"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..2961
                     /locus_tag="O0X12_00795"
     CDS             <1..2961
                     /locus_tag="O0X12_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJK52731.1"
                     /translation="TLLADARRFDEAVSAISHVTLGDREPFLQELKCEWLERIGRYAD
                     AAAVCSHLTNLRPDDKHLRMRLAELTLRSGNFAEAKGMFDQLAATAFDDRGMFLSSAA
                     FCCEMLNDRDGALVRYAGLPENDPTGWYRRARLEESMGKYKDAAASYGMVQQYSADTD
                     ITVTVRRALCLYWDGNEKESASQLEKVLSRGYADAELWYLLGIVSFLSGNMKRASEAF
                     LEMIHLNQSNTSVWYMKGCAEYLSGRYKEAIDSFTRMGRLGGDSGRAPSKMKWFAEED
                     GDMQLFNNPAPVGSASMTKPEIGDVNVGLASMQSFALSALGRYADADKNAVRVLELDP
                     NRLDLQLLHGKCLAAQSNYRQAADVAARVIAKDPTSLAAHELYASSMMCTGHYKEAAE
                     AWEEIIRMVPDNSPAFFGVADAWAGIGRYESAATAYTHLLETMPDDLSLIFGLAGVLF
                     RDGKYADALTYYERAVSLSPATPAAHIGVAQCCEMLGKYAEAGEALISAEQLLPGHPG
                     VLIALARVQSEGGNAEAAVETYLGVLREYPEIPGIAAQVAKLCAALGRNDEAADAVAL
                     ALKDSAESPDLLTLGGDVCTALGRYDEAAEYYSRVAKQMPDNAHVWFGIGHLHLISGD
                     FREASIGMDKVLELEPENTEAMRDKAAALTALGNFPEAADVLRKLIETDGTDTKALLD
                     LASVLEQMQKYDEALEVYGKYLQSGISSSDVIRKLSAIYLLKGEYEEALAGYDMLLAE
                     NEKDLVIIRQRAEVLWYLGQYDSAAAACADLLNERPDDDSVRYLYASALANAGRANDA
                     IEEFSRLVANNADNTAALFAVADLFGRTGRYLESAKCYDKLINLYPKNCIAHLQKAMM
                     LVKVGDIPSSVEAFESAVLVDPKNPSLLLGLAFMQMLCGRPAEALAQLEKAERAGATD
                     PDLFYCRGLICLQQSRFDLAVAAAEKILENHPDHGPAWHLKGRALEAAGHLREALVCF
                     TKVVELDTDPNEEA"
     gene            2962..3438
                     /locus_tag="O0X12_00800"
     CDS             2962..3438
                     /locus_tag="O0X12_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HJK52732.1"
                     /translation="MQIQILCVGRIKDSYLNEGIAEFAKRLRPYATVFVTELSEVKIP
                     ENASSPAEIQVKEREGEKLLAGVKPGFLTVALDPRATLIASEDIAALFGELELSGKNI
                     CFLIGGPLGLSPAVLAAVDRTISFSRLTFTHTMIRLLLFEQIYRGFRILRGEPYHK"
     gene            complement(3526..4203)
                     /locus_tag="O0X12_00805"
     CDS             complement(3526..4203)
                     /locus_tag="O0X12_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HJK52733.1"
                     /translation="MNTILHKPHVLMISEITADGKLTLKRGASSKILMKHMSHEAEIL
                     LHETRAAYDAIMVGSSTIKIDNSFLTVRMVPGKSPLRVIPSSMADLPLDANVLDTSTV
                     PTVVAVSAAAPKDRVAALQEKGVSVVVAGKEKVDLPLLMDILYREFSVRKLIIEGGPT
                     LNWHMLHHHLVDEIRLIHLPFIVGGNDTPSLVGGMHIDSEDQMIRLELSGTRMVGTNM
                     ITEYRVL"
     gene            4321..4968
                     /locus_tag="O0X12_00810"
     CDS             4321..4968
                     /locus_tag="O0X12_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HJK52734.1"
                     /translation="MNITALRPRLIGCIDPIGNAFVRIGLKPNQITFLSLIFGIACAV
                     CYMQRLFLLGSILLGVSAILDLVDGNVARKTNRKSDFGAVLDWIIDKYVDGIVLLGIG
                     LSGTAVISQFLTAPVWSDTAIVGLAIIGSLMNTFIKPVTYAEIGYTKKEDGKISDPLE
                     GIGFFGRPETIICLILFGLISQIWIAVILIAVCTNLSALQRILYLARRHGSYKEE"
     gene            complement(4982..6067)
                     /locus_tag="O0X12_00815"
     CDS             complement(4982..6067)
                     /locus_tag="O0X12_00815"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HJK52735.1"
                     /translation="MDEKTFLCCIEAIKSNDVAIQEDAANAIAALTDERWLPQILHHL
                     NDANPLVRRVMLWTLRNYTGKISYPQFLAYLHDPDMAVREAALLLFMEGEKPACDALV
                     AAVSSENEELQFSAVQALGQFRRPEAIDPLIQAAGAKNPDIREVAVLSLGVYSEKRVV
                     PLLLASLKDEPQIRLAALEGLRGRELSPEEIVTVLECLGDTDFPEIRAAAVAVLGPLV
                     PEEFTEDPSPQVRRVVASVVRSPKLLSKLCGDTDASVRMAAAESLGKQEELQEDVLLP
                     LLTDSVPGVRRAAVTALASSRRPDVVAALIDRLHDPKPGVQAAAATVLGEIGGDEVVA
                     ALTEASKSGNVILRGITKNALNAALKK"
     gene            complement(6086..6367)
                     /locus_tag="O0X12_00820"
     CDS             complement(6086..6367)
                     /locus_tag="O0X12_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HJK52736.1"
                     /translation="MVRVKASHILVKTEAEAKEIIRKLSSGDDFAKLAKLYSQCPSGR
                     AGGDLGYFGKGQMVKPFEDAAFKAKVGEVVGPVKTQFGWHVIKVTDMKN"
     gene            complement(6554..6937)
                     /locus_tag="O0X12_00825"
     CDS             complement(6554..6937)
                     /locus_tag="O0X12_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HJK52737.1"
                     /translation="MTRTAYARENETPMSETMDALAVLGDAVLDLVIICEIMAEGEYD
                     KGEITRKKIDAVNMTVVRRIAEQLDLPSYMRWGKGELRMQIWTSGRVSAECFEALIGA
                     AYMDGGVEAATTIIRTVRTPQRQTP"
BASE COUNT         1648 a         1676 c         1769 g         1884 t
ORIGIN      
        1 acactcctcg ctgatgcaag gagatttgat gaggcagtct ctgccatcag tcacgttacg
       61 ctcggagatc gcgaaccatt tctgcaggag ctgaaatgtg agtggctgga acgtattggc
      121 cgatatgcgg acgcggcagc ggtgtgcagc catctgacga accttcgtcc tgatgacaaa
      181 catctccgta tgcgcttggc ggaacttacc ctgcgttcag gcaatttcgc tgaagcaaaa
      241 gggatgtttg atcagcttgc tgcaaccgcg tttgatgatc gcggcatgtt tttatcgtct
      301 gctgcgtttt gctgtgagat gctgaacgat cgtgatggtg cccttgtgcg atatgccggt
      361 cttcctgaaa atgatccaac cggttggtat cgtcgtgcca gacttgagga gagtatgggt
      421 aaatacaagg atgcggcagc ctcctatgga atggttcagc agtactccgc agacaccgac
      481 ataactgtga cggtgcgtcg tgccctctgc ctttattggg atggaaacga gaaagagtca
      541 gcgtcacagc tggaaaaagt gttgtcacgc ggatatgccg atgcggagtt gtggtatctg
      601 cttggtattg tttccttcct ttctggaaat atgaaacgtg cctcagaggc gttccttgag
      661 atgattcatc tgaatcagtc gaatacatcg gtctggtaca tgaagggatg cgctgagtac
      721 ctttctggaa gatacaaaga ggctattgac agctttaccc ggatgggcag acttggcgga
      781 gatagtggac gcgctccgtc aaagatgaaa tggtttgcag aggaagatgg cgacatgcag
      841 ttgttcaaca accctgcacc ggtaggctca gcttcgatga caaaacctga gatcggtgat
      901 gtcaatgtag gtcttgcttc tatgcagagc tttgcactgt ctgcgcttgg gaggtatgca
      961 gatgcagata agaacgctgt gcgtgtactg gaattggatc ccaatcgcct ggacttacaa
     1021 cttctgcacg ggaaatgtct tgctgcacag tccaattatc gccaggcagc tgatgttgcc
     1081 gcacgtgtga tcgcaaaaga tcccacgtca cttgccgctc atgagttgta tgcgtcgtct
     1141 atgatgtgta cgggtcatta caaagaggct gcagaggcat gggaggagat aatccggatg
     1201 gtcccggaca acagtcctgc attctttggc gtggcggatg cctgggcagg cattggaaga
     1261 tacgaatcag cagcaacagc atatacccat ctgctggaga cgatgcctga tgatctgtcg
     1321 ctgatctttg gtttggcagg tgttctgttc cgcgacggaa aatatgctga tgcacttacc
     1381 tactatgagc gtgctgtttc tctgtctcct gcaacacctg cggcacatat cggtgttgca
     1441 cagtgttgtg agatgcttgg aaaatatgct gaagctggtg aggcgcttat ttctgcagaa
     1501 cagcttttgc ccggtcatcc tggcgtgctg attgcgcttg cacgggtcca gtcggaaggt
     1561 ggcaatgccg aggcagcagt agaaacatat ttgggagttt tgcgtgagta tccggagatt
     1621 ccgggtattg ctgcccaggt ggcaaagctg tgcgcggccc ttggcagaaa tgatgaggcg
     1681 gcagatgctg tggcacttgc actgaaggat tctgcggaga gccctgacct tctgacactt
     1741 ggcggtgatg tctgtacagc actcggcagg tatgatgagg ccgcagagta ctactcccgt
     1801 gttgcaaaac agatgccgga caatgcccat gtctggtttg gtatcgggca cctgcatctg
     1861 atcagtggtg acttccgcga ggcatcaatc ggaatggaca aagttctgga acttgagcca
     1921 gagaacacgg aagcgatgcg tgacaaggca gctgccctca cagcactggg taacttcccg
     1981 gaagctgcag atgttttgcg aaagctgatc gaaacggatg ggactgatac aaaggcactt
     2041 ctggatcttg caagtgttct tgagcagatg cagaagtacg atgaggcact tgaggtgtat
     2101 ggcaagtatc tgcagagcgg tatttccagt agtgatgtga tcaggaaact ttcggcgatt
     2161 tatcttctca aaggcgagta tgaagaggca cttgcgggat atgacatgct gctcgcagag
     2221 aatgagaagg atcttgtgat cattcgccag cgtgcagagg tgttatggta tctcggccaa
     2281 tatgactctg ctgctgccgc ctgcgcagat ttgctgaatg agcggcctga tgatgactcc
     2341 gtgcggtact tgtatgcatc ggctcttgca aatgcaggaa gagcgaacga cgcaattgag
     2401 gaattttcac gtcttgttgc aaacaatgcg gacaacacgg cggccctgtt tgcagttgca
     2461 gatctgtttg gccgcacagg aagatatctg gaatctgcaa aatgctacga taaactgatc
     2521 aacctatacc cgaagaactg tattgcacat ctgcaaaagg caatgatgct ggtaaaagtt
     2581 ggtgacatcc cgtcttcagt tgaggcattt gagtctgcgg ttctggttga tccgaaaaac
     2641 ccgtctctgt tgttgggtct tgcgtttatg cagatgctgt gtgggcggcc agcggaggca
     2701 cttgcccagt tggagaaggc ggagcgtgcg ggtgctacgg atccggatct gttctactgc
     2761 cgcggtctga tctgtcttca gcagagcaga tttgatcttg ccgttgctgc tgctgagaag
     2821 atcctggaga accacccgga tcacggtcca gcctggcacc tgaaaggacg tgcacttgag
     2881 gccgcaggtc atcttcgcga agcacttgtg tgtttcacga aagttgtgga gctggataca
     2941 gacccaaacg aggaagcctg aatgcagatc cagatcctgt gtgtcggcag gattaaggac
     3001 agttacctga atgagggaat cgccgagttt gcaaaacggc tgagacccta tgccactgtt
     3061 tttgtgacgg agctttcgga ggtgaaaatc cctgagaatg catcatcacc tgcggagatc
     3121 caggtaaagg aacgggaagg tgaaaagctt cttgccgggg taaaacctgg gtttctgacg
     3181 gttgcattgg atccacgtgc cacactcatt gcaagcgaag acattgccgc attgtttggg
     3241 gagttggaac tttctggaaa aaatatctgt tttcttattg gcgggccgct aggactgtca
     3301 cctgcggttc ttgccgcggt ggatagaacg atctcatttt cccgcctgac atttacacat
     3361 actatgatcc gtctgctttt atttgaacag atctaccgag gtttccggat tctgcgtggt
     3421 gaaccatacc acaagtgatc tggaattgtg tggcaaaaac gtgggggagg cggaggagtg
     3481 tgtactgcac ctaatcactc cccctgatga tttatatttc ctacctcaga gtacccgata
     3541 ctctgtgatc atatttgttc caaccatgcg tgttcctgaa agttcaagcc ggatcatctg
     3601 atcctccgag tcaatatgca tccctccaac cagtgaggga gtatcatttc ctcccacaat
     3661 aaacggaaga tggattaatc ggatctcatc caccagatgg tgatgcagca tatgccagtt
     3721 cagtgttggc cctccctcaa ttatcagctt tctcactgaa aattcccggt ataaaatatc
     3781 catcagcaga gggaggtcca ctttttcttt accggcaacc acgacagaca cgcccttttc
     3841 ctgaagggcc gcaacgcgat cttttggtgc tgctgctgac acggcgacaa ctgtcggaac
     3901 agttgatgtg tccagtacgt ttgcatccag cgggaggtct gccatgcttg agggaatcac
     3961 ccgcagagga ctttttccag ggaccatacg tactgtcagg aatgagttat cgattttaat
     4021 tgtggatgat ccaaccatga tggcatcata ggcggcacgc gtctcatgaa gcagaatttc
     4081 cgcctcatgc gacatatgtt tcatcaaaat tttgctggac gcaccgcgct ttagggtaag
     4141 tttcccatct gctgtgatct cagagatcat taacacgtgg ggtttatgca gtatcgtatt
     4201 catgaagtat ttcctcttga ttcatatagg aataaaagct aaagaatatt tggtatagaa
     4261 atcccggtca aaggtgcatc atttccactc ccgtcagata tctgatgcca gcaggttatc
     4321 atgaatatta ccgctcttcg tccacggctg attggctgta ttgatccaat cggcaatgcc
     4381 ttcgtccgga ttgggcttaa accaaatcag attacattcc tctcccttat ttttggtatt
     4441 gcctgtgctg tctgttatat gcaacggctg tttctccttg ggagtatcct gcttggggtc
     4501 tctgcaatcc ttgatctcgt cgacggaaat gttgctcgga aaaccaatcg taaaagtgat
     4561 tttggtgcag tacttgactg gatcattgac aaatacgtgg acggaattgt tcttcttggg
     4621 attggtcttt ccgggactgc tgtcatctct cagttcctca cagcccctgt ctggtctgat
     4681 accgcaatcg tcggccttgc tattatcggt tccctcatga ataccttcat caaaccggta
     4741 acctatgctg aaatcggcta cactaaaaag gaagatggta aaatcagtga tcctttggaa
     4801 gggattggat ttttcggcag acctgaaact atcatctgtc tcatcctgtt tggactgatt
     4861 agccagattt ggatagcagt aatcctcatt gctgtctgta caaatctctc agctctacaa
     4921 agaattctct accttgcacg ccgccacggt tcctacaagg aagagtgaaa ttaagatatt
     4981 tttatttttt cagagctgca ttcaatgcat tttttgtaat tccgcgcaga atgacgttac
     5041 cactctttga tgcctcggta agtgctgcaa caacctcatc accaccaatc tcgcccagta
     5101 cggtcgctgc tgcagcctga acaccgggct ttggatcatg cagacggtca ataagtgcgg
     5161 caaccacatc aggccttcgc gatgatgcga gtgctgtaac cgcagcccgt cgaacccctg
     5221 gtactgaatc agttagtagc ggcaacaaca catcctcttg gagttcttcc tgttttccca
     5281 gtgattctgc cgccgccata cgaactgagg catctgtatc cccgcacaac ttggaaagaa
     5341 gttttggtga tctgactact gatgcaacga cacgccggac ctgtggactt ggatcttctg
     5401 taaattcttc agggaccaag ggaccaagta ctgcaactgc tgcagcacga atttccggaa
     5461 aatcggtgtc acccaggcat tctaaaacgg ttactatctc ttcaggagac aactcccgtc
     5521 cccgcagacc ctcaagcgca gcaagacgaa tctgcggctc atctttcagt gatgcaagaa
     5581 gtagagggac cactctcttc tcggagtata caccaaggga tagtactgca acttcacgaa
     5641 tgtctggatt ttttgcacca gctgcctgga tcagagggtc aattgcctct ggtctccgga
     5701 actgtcctag tgcctgaact gcggaaaact ggagttcttc gttttcggaa gatactgcag
     5761 ctaccagtgc atcacacgca ggtttttccc cttccataaa caaaagcagc gcagcctcac
     5821 gcacggccat atcaggatca tggagatagg caagaaactg tggatacgat atttttcctg
     5881 tgtagttacg cagcgtccag agcatcaccc tgcgtacgag agggtttgca tcattgaggt
     5941 ggtgcaaaat ttgcggcagc cagcgctcat ccgttaacgc tgctatggca tttgccgcat
     6001 cctcctgtat cgccacatcg ttgcttttta ttgcttcaat gcagcacaaa aaggtctttt
     6061 cgtccattgc cttctgacag tgtatctagt ttttcatatc tgttactttg atcacatgcc
     6121 aaccaaactg cgttttgacg ggccctacaa cctcaccaac tttagcttta aatgccgcgt
     6181 cctcaaaggg ttttaccatt tgtcctttac cgaagtatcc cagatccccg cctgctcttc
     6241 cagatggaca ttgggagtag agctttgcaa gctttgcaaa atcatccccg ctggaaagct
     6301 tccttataat ctcttttgcc tctgcctctg tctttaccaa aatatgggat gcctttaccc
     6361 gtaccatacc atagcatatc gcactgcaac gcaataaact atgatagcac gctagttatg
     6421 ttacaccatg tattctctat ctcatgcaaa tttgtattgt ctaaattatc aaaaaaagaa
     6481 ctggtgatat gggaggatat tgcaatctat ctggtatgtt ctgttctcga ggtatcacag
     6541 ttcattgatg gaattagggt gtctgtcttt gcggtgtacg aacggttctg atgattgtcg
     6601 ttgctgcctc aacacctccg tccatgtatg cggcaccaat cagtgcttca aagcactctg
     6661 ctgaaacacg tccactcgtc cagatctgca tgcggagctc accttttccc catcgcatgt
     6721 atgaaggaag atcgagttgt tcggcaattc tccggacgac cgtcatattg acagcatcaa
     6781 tctttttacg cgtaatctcc cccttgtcat actctccttc tgccattatc tcacagatga
     6841 ttaccagatc cagtactgca tccccgagta ctgcaagtgc atccatggtt tcactcatcg
     6901 gcgtctcgtt ctcacgggcg taggcggttc gtgtcattgc ctggttgaga tacgcttcat
     6961 ttttgaatgt gtatcct
//
LOCUS       DALTZZ010000049         2682 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_6939,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000049 DALTZZ010000000
VERSION     DALTZZ010000049.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6939"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /gene="radC"
                     /locus_tag="O0X12_00830"
     CDS             <1..657
                     /gene="radC"
                     /locus_tag="O0X12_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HJK52738.1"
                     /translation="REKLIANGPQSLTLAELIAAIIGRGTVKNDALKIGKAVADILME
                     HTYTTSIQDVAAVDGMGPAKACQILAALELARRFPPPDRKSAKIRCAEDLLPFVSQYR
                     YDQQENVIAATLSGAHEILNIRVVTRGLVNQSQIHPREVFADAVTDRAAAIILVHNHP
                     SGNLTPSTRDILMTRTIQEAGEILGIRLLDHLIIGPCDGFLSVLEAFENDTHENQDLM
                     "
     gene            686..1696
                     /locus_tag="O0X12_00835"
     CDS             686..1696
                     /locus_tag="O0X12_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HJK52739.1"
                     /translation="MPSTIIVGGFFGDEGKGKIVAHIAQQDKATIIARGGVGPNAGHT
                     VEVGDKKYGVRMVPSGFVYPHARLMIGSGVLVDPRVFAHELEVLGCRDRTFIDGRCGI
                     IEEEHIYKDKHDDHLSKTVGSTGSGCGPANSDRVMRTARLAKDVPELAPYIIDVAQNV
                     NDAIDAGDSVIIEGTQGFGISLYYGNYPFVTSKDTSASQMAADVGVGPTKIDDVVVVF
                     KAFPTRVGEGPFPTELTHEQSLAMGIQEFGTVTHRERRIGTWDGKMARYSAMINGCTA
                     IAITGVDHIDKDVFGVKEYSKLTPKVREFLEQIESDTGCPVGIISTGPEQSQIIDIRS
                     EL"
     gene            1700..1879
                     /locus_tag="O0X12_00840"
     CDS             1700..1879
                     /locus_tag="O0X12_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HJK52740.1"
                     /translation="MKRWVLDIICCPVCKGNFMLTEMEGNDTDIVEGLLTCTSCKRVY
                     PISSGIANLLPNQED"
     gene            1891..2436
                     /locus_tag="O0X12_00845"
     CDS             1891..2436
                     /locus_tag="O0X12_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52741.1"
                     /translation="MAIVEIQLNRLGINSLELSTDTVDVSAGTALHVRFVNHGSPTHA
                     TLRCEASAYTDFTYENIYVEGESELEIKMKEEAGTGSFDMQVITGYGMRREAFTIHVL
                     KSCPAPVPEPVIFPEEPTEDPKKGSFGQKAAMILIMPIIAAIIIALWQFGLLNIDGHA
                     MTIIVYLVMLAGVIVAWRSAQ"
     gene            2418..>2682
                     /locus_tag="O0X12_00850"
     CDS             2418..>2682
                     /locus_tag="O0X12_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HJK52742.1"
                     /translation="MAFGAIILFCAVVLDRLIGDPRSRLHPVALLGTVIGWWGKTNTY
                     PKRLERITGIVGWLITVLIFILPFLVICLIPETYWYIQLPLAAV"
BASE COUNT          680 a          687 c          713 g          602 t
ORIGIN      
        1 cgggaaaagc tgatcgcaaa cgggccgcaa agcctgacac tcgcggaact gattgcggcg
       61 atcatcggac gcggaaccgt aaaaaacgac gcactcaaga tcggcaaagc tgttgcagac
      121 atcctgatgg aacacaccta tacaacatcc atacaggacg tagccgctgt tgacggaatg
      181 ggaccggcaa aggcctgtca gattcttgca gccttagaac ttgcccggcg ttttcccccg
      241 ccggatcgaa aatccgcaaa aatccgatgt gcagaggatc ttctgccgtt cgtcagtcag
      301 taccggtatg atcagcagga aaacgtcata gcggcaacac tgtcaggggc gcatgaaatt
      361 ctcaacatcc gggtagtgac aagaggcctc gtgaaccaga gtcagattca tccccgtgaa
      421 gtgtttgcag acgcagtaac cgatcgggca gcagcaatta tcctcgttca caaccatccg
      481 tcgggaaacc tcacgccaag tacacgggac attctcatga ccagaacgat tcaggaagca
      541 ggggagattc ttggaatcag actgctggat cacctcatca tcggaccgtg cgacgggttc
      601 ctgagcgtac tcgaagcttt cgagaatgac actcatgaaa accaagacct tatgtgattg
      661 agccgcacat agatattgat acagtatgcc aagtacgatc attgtcggcg gtttttttgg
      721 tgacgaagga aaaggaaaga tcgtcgccca cattgctcag caggataaag caacaattat
      781 tgcacgcggc ggtgtcgggc cgaatgccgg ccacaccgtt gaagtgggag ataagaaata
      841 tggcgtccga atggtgccgt ccggctttgt ctatccccac gcaagactta tgatcggcag
      901 cggagttctt gttgacccgc gggtctttgc ccacgagctg gaggtgcttg gatgcagaga
      961 ccggaccttc attgacggtc gctgcggaat catcgaagag gaacatatct acaaagacaa
     1021 acacgatgat cacctctcta agacggttgg atcaaccggc tccggctgtg gcccggcaaa
     1081 ctccgatcgt gtcatgcgca cggcacgtct tgcaaaggat gttcccgaac ttgccccgta
     1141 catcattgat gttgcccaga acgtgaacga tgcaatcgac gcgggtgact cggttatcat
     1201 cgagggtacg caagggttcg gtatttccct ctactatgga aactatccgt tcgtgacaag
     1261 taaagacacc tccgcatcgc agatggcagc cgatgtcggt gtcgggccga ccaaaatcga
     1321 tgatgtggtt gtggtcttca aggcattccc gaccagagtc ggtgaaggac cgttccccac
     1381 cgaactcacc catgaacagt cccttgcaat gggtattcag gagtttggta ccgttacgca
     1441 ccgcgagcgg agaatcggta catgggacgg aaagatggca cgttactccg caatgatcaa
     1501 cggatgtacg gccatcgcca tcaccggtgt tgaccacatc gacaaggatg tgtttggtgt
     1561 caaagagtac agcaaactca caccgaaggt ccgtgagttc cttgaacaga tcgagtctga
     1621 cacaggctgc ccggttggta ttatctcaac tggtcctgaa cagtcccaga tcattgatat
     1681 cagatccgag ctgtaaacca tgaaacgctg ggtcctcgat attatctgct gtcctgtctg
     1741 caagggaaac tttatgctta cagagatgga gggtaatgat acagatattg tcgaaggact
     1801 gctgacctgc acatcctgca agcgggtgta tccgatctcc tccggcattg caaaccttct
     1861 cccaaatcag gaggactgag gtgctctact atggcaattg ttgaaattca gctgaaccgc
     1921 ctggggataa attcccttga actatccacc gataccgtgg atgtaagcgc aggaaccgca
     1981 ctgcacgtcc ggtttgtgaa tcacggatct ccgacacacg caacactgcg gtgcgaggca
     2041 tcagcttaca cggactttac gtacgaaaac atctacgttg agggcgagtc ggagcttgag
     2101 atcaagatga aggaggaggc gggaaccgga agtttcgaca tgcaggtcat taccggctat
     2161 ggtatgcgcc gcgaggcgtt cacgatccat gtgctgaagt cctgtcctgc cccggtaccg
     2221 gaaccggtta tcttcccgga agaaccgaca gaggatccga aaaaaggatc ctttggtcaa
     2281 aaagcagcga tgatactgat tatgccaatc atcgctgcaa tcattattgc attgtggcag
     2341 tttggtctcc tgaatattga cggacacgcc atgacaatta ttgtgtacct tgtaatgctc
     2401 gccggagtca tcgttgcatg gcgttcggcg caataatcct tttttgtgca gtagttctgg
     2461 atcggctgat cggtgatccg cgatcgcgcc tgcacccggt agctctcctg ggaactgtga
     2521 tcggctggtg gggaaaaaca aatacctatc caaaacggct agaacggatc accggtattg
     2581 tagggtggct gataaccgtt ctcatcttta tcctgccgtt ccttgtgatc tgtctgattc
     2641 cagagacata ctggtatatt cagctgcccc ttgcagcagt ac
//
LOCUS       DALTZZ010000050         2984 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_7368,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000050 DALTZZ010000000
VERSION     DALTZZ010000050.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7368"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="O0X12_00855"
     CDS             complement(<1..1232)
                     /locus_tag="O0X12_00855"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HJK52743.1"
                     /translation="MNKLHLVLVLLVAVVMLTVPAAAEVALGGSVGGSTATISVTSSP
                     SGASVYDAGVYQGTTPCNIIVHTTGTPIDHDIVISKNGYYDYHQYIGTVVTDQYITVN
                     AVLTPVVQTGYLDVSSSPSGANVYIDGIYKGTAPLVVSVDAGSHSVRLEKPGYNTWYG
                     TYSVSAGKTVQVAASLSASVTYGYINVHSYPSAADVYIDGNYRDDTPAVITVTAGTAH
                     EVKVVHSGYEVYRQTVTVSSGSTVSIDANLVASGDAYIQLASYPAGAAVYVDGSYCGN
                     TGYSSSSSVNYMHIGPLSAGTHSIMLKKDGYNTYTSTVTLSPNEVRTLSVTLTPTSPT
                     PSGYSSLSLSSTPSGADVYVDNVFRGYTPIYLSDIPAGQHTLLLQHTGYNDWTEYVSF
                     TSDYTVEKDVTMSPTSVPP"
     gene            complement(1279..1593)
                     /locus_tag="O0X12_00860"
     CDS             complement(1279..1593)
                     /locus_tag="O0X12_00860"
                     /inference="COORDINATES: protein motif:HMM:NF020406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1805 domain-containing protein"
                     /protein_id="HJK52744.1"
                     /translation="MQIEYLSPDHALITDAGVSVDAYCLELGSANLIFVKTSSGIVGC
                     GFFDLATFEKFGIPAAKVTGISTIEDLLKGSVSAATPAAVLRGVNPGMSGEEAVRILS
                     RS"
     gene            1684..2175
                     /locus_tag="O0X12_00865"
     CDS             1684..2175
                     /locus_tag="O0X12_00865"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HJK52745.1"
                     /translation="MIREIRLYGDPVLTKSAETVPEITEEIRDILTDMWDTMEANRCV
                     GLSAPQIGVSLRLFVINAGGVVIQGANPEVLTEGAPSEDIEGSPCIPGIQKPVRRPRK
                     ITVRYLDESGENVERELKGVAARAYLHEKDHHEGVLFIDHLKPGVRKLIQKDLDRIAD
                     NRA"
     gene            2225..2518
                     /locus_tag="O0X12_00870"
     CDS             2225..2518
                     /locus_tag="O0X12_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin:thioredoxin reductase"
                     /protein_id="HJK52746.1"
                     /translation="MTTPPTPEEIAALIPKIRADSEKLAQRKGWRLNPDTALADSVIA
                     GLARNKLMRGKRYCPCRLPSGNADVDKLFICPCRDAENDIETEGHCHCFLYMK"
     gene            2629..>2984
                     /locus_tag="O0X12_00875"
     CDS             2629..>2984
                     /locus_tag="O0X12_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HJK52747.1"
                     /translation="MAGCVDTPAEDGKKTYVVGIDGEYKPFSYLGDNGEFIGFDVDSV
                     KWIAEQKGFNVEIQAVAWDGIIPALQTGKIDMVYSGMSITPDREEKVNFTIPYWQVNQ
                     SVVALADSEVTMDQFT"
BASE COUNT          855 a          669 c          761 g          699 t
ORIGIN      
        1 ggcggaacag atgtggggga catggtcacg tccttctcca ctgtataatc agaggtgaag
       61 gagacatact ctgtccagtc attgtatccg gtatgctgga gtagtaaagt atgttggcct
      121 gcggggatat ccgaaagata aatcggtgtg tatccgcgga agacattgtc cacatacacg
      181 tcagcacctg atggtgttga ggaaagcgac agactggaat aaccagacgg tgttggtgag
      241 gttggtgtca atgttacact cagggtacgg acttcgtttg gagacagtgt aactgttgag
      301 gtataggtgt tatatccgtc tttcttcagc ataatcgagt gggtgcctgc tgacaacgga
      361 ccgatatgca tgtaattcac agaagatgac gacgaatatc cggtgtttcc acagtaggaa
      421 ccatcaacat acactgctgc accagctgga tatgatgcaa gctgtatgta agcatctccg
      481 cttgctacca ggttggcatc aattgagaca gtactaccgg aactgaccgt aacggtctgt
      541 ctgtacacct cgtatccgga gtgaaccact tttacttcat gtgcagtacc ggcagttaca
      601 gtaatcacag ccggcgtatc atcacggtaa tttccgtcaa tgtacacatc tgctgcggat
      661 ggatacgagt ggacattgat gtatccgtac gtcacggatg ctgaaaggga agcagcgacc
      721 tgtactgtct ttccggcgga aacactgtac gttccatacc aggtgttata tccgggtttt
      781 tccagccgga cagagtgact tccggcatca acagatacca ccagcggagc agttccttta
      841 tagataccgt caatgtacac gtttgcaccc gaaggagatg acgagacatc aagataacca
      901 gtctgtacaa ctggtgttaa tactgcattc accgtgatgt actgatcagt cacaacagta
      961 ccgatgtact gatgataatc atagtatcca tttttcgaga ttacgatatc atggtcgatt
     1021 ggtgtacctg ttgtgtgaac gataatatta cacggcgtcg taccctgata gactccggca
     1081 tcatacacag acgcacccga gggcgaagag gtcacactaa tcgtcgcggt ggaaccgcct
     1141 acagaaccac cgagggcgac ctcagctgcc gctggtacag tcagcattac aactgcgacc
     1201 agcaacacca gaacaaggtg aagtttgttc atgataaaag tatcaacgtt gccagagata
     1261 aaaaaagaat cggtcagatt agctacggct tagtattctg acagcctcct caccactcat
     1321 acccgggttt acccccctga gaactgctgc aggggtagct gcggaaacag atcccttaag
     1381 aagatcctca atggtcgaga tcccagtaac ttttgcggca ggaatgccaa atttttcaaa
     1441 agtggcgaga tcaaagaagc cgcaaccgac gattcctgaa gatgttttta caaagatcag
     1501 gttcgcagac ccaagttcaa ggcaataagc gtcaacagaa acaccggcat cagtaatgag
     1561 ggcatgatca ggggagagat actcgatttg cataccgagt tcatttcaca ttctttccta
     1621 aaacagttta cccaaccatc ggatacgcaa aaggtatggg actgggctgc acagatctat
     1681 aatatgattc gggagatacg gctgtacggt gacccggtac tgacaaaatc agcagaaacg
     1741 gtaccggaga taacagagga aatacgggat atcctgactg acatgtggga tacgatggag
     1801 gcaaaccggt gtgttggtct ttctgcaccg cagattggag tttcacttcg gctgttcgtg
     1861 atcaacgcag gaggggttgt gatccaaggt gcaaaccccg aagtccttac cgagggagca
     1921 cccagcgagg acatagaggg aagtccctgt attcccggaa tccagaaacc ggtacggcga
     1981 ccgcgaaaga tcacggtgcg gtacctggat gaaagtggtg agaatgttga acgtgaacta
     2041 aagggtgtcg ctgcacgggc atatctgcat gaaaaggatc atcacgaagg agtacttttt
     2101 attgatcatc tcaagcctgg tgtccgaaaa ctgatccaga aagatctgga tcgaattgca
     2161 gacaacagag cataaagtcc ccaaaccaaa aacagatatc ctccaaaagg gaaaaataga
     2221 ggtcatgacc accccgccaa caccggaaga gatagcagca ctgataccaa aaatccgtgc
     2281 ggattccgaa aaactcgcgc aaagaaaagg atggagacta aatcccgaca ctgcacttgc
     2341 ggattcagtc attgcagggc ttgcacgcaa taaactgatg cggggaaaac gatactgtcc
     2401 atgcagactt ccaagcggca acgcggatgt tgacaaactg tttatttgtc cctgccgtga
     2461 tgcagagaat gatattgaaa cagagggaca ctgccattgc ttcctctata tgaaatgatt
     2521 gattttcttc gcggaaagta atttatattg attaaccccc atcactatag gatggataaa
     2581 aaagtgttgt atggggtagc agggctgtgc cttgtttttg ctgtcctgat ggcagggtgt
     2641 gtagacactc ctgctgaaga cggcaaaaaa acctatgttg ttggtattga tggagaatac
     2701 aagccgttct cataccttgg cgacaacggc gagttcatcg gttttgatgt tgactctgta
     2761 aagtggattg cagagcagaa aggattcaat gtcgagattc aggctgttgc atgggatggt
     2821 atcattccgg cactgcagac cggaaagatt gacatggtat actccggtat gtccattact
     2881 cccgaccgtg aagagaaggt aaacttcacc atcccgtact ggcaggtcaa ccagagcgtt
     2941 gtagcactgg cagattctga ggtaacaatg gatcagttta ccaa
//
LOCUS       DALTZZ010000051         3631 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_7495,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000051 DALTZZ010000000
VERSION     DALTZZ010000051.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7495"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="O0X12_00880"
     CDS             <1..199
                     /locus_tag="O0X12_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HJK52748.1"
                     /translation="KKIGFAFGSNGGKGGAPKVITELLEKAGIQIWHDPIEFTYRPDQ
                     ADKEKFFALGQEIAKEIKKMP"
     gene            409..1428
                     /gene="asd"
                     /locus_tag="O0X12_00885"
     CDS             409..1428
                     /gene="asd"
                     /locus_tag="O0X12_00885"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HJK52749.1"
                     /translation="MIHVGVLGATGAVGQRFVQLLANHPWFTLSTLTASDRSAGKTYG
                     SVVNWRLDSPFPESVKDIVVSPTTVDAVKDCDIVFSALPADIATTLESEIADAGVGVF
                     SNASSHRMEKDVPLMIAEVNPGHAALIDVQKKNHNREGFIVTNPNCSTIMLATALAPL
                     RKFSFTNINVATMQAISGAGFDGVPAFGIYDNVIPYIGSEEEKMAREANKILGTFNGS
                     QITGAPFQVSASCNRVPVVDGHTLNVWIDIAATPDEVIEAFQTWQPPFTGLPTQPEKT
                     ILYLPEENRPQPRLDRNRGNGMTVSVGRVRDGIRFVAMGHNTIRGAAGASVLNAELLH
                     TKKYF"
     gene            1541..1807
                     /gene="albA"
                     /locus_tag="O0X12_00890"
     CDS             1541..1807
                     /gene="albA"
                     /locus_tag="O0X12_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HJK52750.1"
                     /translation="MDNTVLIGNKPVMNYVLAVVTQFNAGNSEVIVKARGKAIVRAVD
                     TAEIVTRQFLPNVVKQSVTTSTDQVEMDNGPSNVSCIQIILAKS"
     gene            1819..2118
                     /locus_tag="O0X12_00895"
     CDS             1819..2118
                     /locus_tag="O0X12_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52751.1"
                     /translation="MSKKNTIAGVALVVACVLCVWYVSLPSTEEFSREGTVSLLGEKT
                     VISGDDGTVYIPSTNVLEDCEIGTRIWFTAVVNSSQSELLRGPYKPVDILNISCM"
     gene            2128..3381
                     /locus_tag="O0X12_00900"
     CDS             2128..3381
                     /locus_tag="O0X12_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HJK52752.1"
                     /translation="MYPIAVGILAVVFLLIVMRRIGHVRLPIWAVMTAGAALALVTGS
                     ITPEAALAAVNIDVIIFLFGMFVLGVAMERSGLLHTASVAAFAKASSRHQVMFWFIFL
                     MAGASAFLMNDTVAIIGTPVALYCSHRFGIPVTRMLVALAAAVTFGSVMTPIGNPQVL
                     MIAMSGGVPNAFGTFFVYLCVPALVSLYILYRMMIWHLPNTEVSSMYDRDSSSPEDPV
                     LERLTKVSLILVFVVICLRIGLSFTGYELPLVLIALAAALPLLLLSRWRVELLRGVDW
                     STLLFFISMFVLMAAVWNTGVIQSLLPESFTTSMPVLFGVSVLVPQLVSNVPYVALVL
                     PVLESAAAPVYLYLTLAAGSTISGTLTILGAASSVIIIQNAEKHGETMTFWEYFRVGI
                     PLTIAASLVFIGWIWICSLLFGATG"
     gene            complement(3399..>3631)
                     /locus_tag="O0X12_00905"
     CDS             complement(3399..>3631)
                     /locus_tag="O0X12_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HJK52753.1"
                     /translation="PKCGTASPAGTKFCPECGTSMVQPESGRCTNCGAVLQPGAKFCP
                     ECGTKVGSPKCPACGSEVAAGAKFCPECGQKL"
BASE COUNT          862 a          868 c          941 g          960 t
ORIGIN      
        1 gaaaaagatc ggatttgcct ttggatccaa tggtggaaaa ggtggagcac ccaaggtcat
       61 taccgagctc ctggaaaagg caggaattca gatctggcac gacccaatcg agtttaccta
      121 ccgtcccgac caggcagaca aggagaagtt ctttgccctt ggtcaggaga tcgcaaaaga
      181 gatcaaaaag atgccgtaaa gcagggaaac aattcccgca tcttttttta cagaatttat
      241 gagaaagctg gcgattgtct aggcagccgg attgtaacct ctgcgattca aaattcacga
      301 cacatggcag gttaccataa caattttccc gggattctgg aaggttatgc acagtctcgg
      361 ggcacccgga cgaaaacatg aatatccggc aaagcctatg aatatctcat gattcatgtt
      421 ggcgttcttg gagcaaccgg cgctgttggt cagcgttttg tccagctcct cgcaaaccat
      481 ccctggttta ccctttcaac ccttactgcc tccgatcggt ccgcaggaaa gacctacggt
      541 tccgttgtaa actggcggct tgactccccg ttcccggagt cggttaaaga catcgttgtc
      601 tctccaacca ctgttgatgc ggtaaaggac tgtgatattg tgttttccgc acttcccgcg
      661 gacattgcaa caacgcttga gtccgagatt gcagacgcag gtgtcggtgt cttcagtaat
      721 gcgagttccc accgcatgga aaaagacgtt cccctaatga ttgccgaggt taatccaggg
      781 catgcagcac tcatcgatgt gcagaagaaa aaccacaatc gtgagggatt catcgtcaca
      841 aaccccaact gttccacgat tatgcttgca acggcacttg ccccgctgcg gaagttttct
      901 ttcacaaaca tcaacgttgc aaccatgcag gcaatctccg gagccggttt cgatggggtc
      961 ccagccttcg gaatctacga caacgtgatt ccatacatcg gatctgagga ggaaaagatg
     1021 gcccgcgaag cgaacaaaat tcttggtacg ttcaatgggt cacagatcac cggagcaccg
     1081 ttccaggtct ctgcatcctg taaccgtgtc cctgttgtag atgggcacac gctgaatgtc
     1141 tggatcgata ttgctgcaac tccagatgag gtcattgaag cattccagac ctggcagccg
     1201 ccgtttactg gactgcctac tcagccggaa aaaacgattc tgtaccttcc ggaggagaac
     1261 cgtccacagc caagacttga ccggaaccgc ggtaacggca tgaccgtttc tgttgggcgt
     1321 gtacgtgatg gtatcagatt cgttgcgatg ggacacaaca ccatccgcgg tgcagcaggt
     1381 gcttcggttc tgaacgccga actgctgcat acaaagaagt acttctaaga cggctctttt
     1441 ggagactatc tgggatattc ccaagattcc cagatacttt cctgtatcca tgcagatacg
     1501 atcaactgat aacagattat cgctaaatga tactacgacg atggataata cagtcctgat
     1561 tggaaacaaa ccggtgatga actatgtact ggctgtggtt acacagttca atgccgggaa
     1621 ctccgaggtg atcgtgaagg cacgcggcaa agccatcgtt cgtgctgtag atacagccga
     1681 aattgtgacc cgccagtttc ttccgaatgt ggtgaaacag agtgtaacca cctccacaga
     1741 tcaggtcgag atggacaatg gcccctccaa tgtctcatgc attcagatca tcctcgcaaa
     1801 atcctgacct ttttttctat gtcaaaaaag aatacgatag ctggtgtggc actggttgtt
     1861 gcctgtgttc tctgcgtgtg gtatgtgagt ctgccaagta ctgaggagtt ttcccgtgag
     1921 ggaacagttt ctctgcttgg ggaaaagacc gttatttccg gggacgacgg gacagtgtat
     1981 attccttcga ccaatgtttt ggaggattgt gagattggaa cccggatctg gttcacagca
     2041 gttgtgaatt cctcacaaag cgaactactg cgaggaccct acaaaccagt agacatcctg
     2101 aacatttcct gcatgtaggc ctgttgtatg tatcctattg ccgttggtat tcttgcagtt
     2161 gtatttctgc tgattgtgat gcggcggata ggacatgtgc gtctgccaat atgggcagtg
     2221 atgacagcag gagcagcgct tgcccttgtt accggttcga ttacaccaga ggctgcactt
     2281 gctgcggtga acattgatgt gatcattttt ttgttcggga tgtttgtgct gggtgttgca
     2341 atggagagat caggtctgct gcatacagca tcggttgcgg catttgcaaa agcatcctcc
     2401 cgacaccagg tgatgttctg gtttattttt ctgatggcag gagcatctgc atttctgatg
     2461 aatgataccg ttgcaatcat tggaacaccg gttgcactgt actgttccca ccgctttggg
     2521 attcctgtaa cacgaatgct ggtagcactc gcagcggcag taacattcgg gagtgtgatg
     2581 acgccaatcg ggaaccctca ggtactgatg attgcaatgt cggggggagt gcccaatgca
     2641 tttggtacat tctttgtgta cctgtgtgtg ccagcactcg tgagtttgta cattctttac
     2701 cgtatgatga tatggcatct gccaaacaca gaggtatcat cgatgtatga tcgtgattcc
     2761 agcagtccag aggatcctgt actggagcga ctgaccaaag tatcgcttat tctggtgttt
     2821 gtagtgatat gtttgcgaat tggactgtcc tttaccggat acgagttgcc tctggtgttg
     2881 atcgcactcg ctgcagctct gccgcttctg cttttgtcca gatggagagt ggaacttttg
     2941 cgtggggtgg actggagtac actgctgttc tttatctcga tgtttgtact gatggctgca
     3001 gtgtggaaca ccggtgtgat tcagagcctg ctgccggaat cgtttacaac gtcaatgccg
     3061 gttctgtttg gtgtgagtgt tctggtacct cagctggtgt caaatgtacc gtatgtagca
     3121 ctggttctgc cggttcttga gtcagcggca gcaccagtct atctgtacct tacacttgct
     3181 gcgggaagca caatctcagg tacgctgaca attttaggtg ccgcaagctc ggtaattatc
     3241 atccagaatg cggaaaaaca tggggaaaca atgacattct gggagtattt ccgcgttggg
     3301 ataccgctga caattgcagc atcactggtg ttcatcggat ggatctggat ctgttcgctg
     3361 ctatttggtg ctactggtta aaagatttaa aaaaatggtt agagtttctg tccgcattca
     3421 ggacagaact ttgcacctgc agcaacctca ctgccacagg caggacattt cggggatccc
     3481 actttggttc cgcactcagg acagaatttc gcaccgggct gaaggactgc accgcagttt
     3541 gtacatctcc ccgattctgg ctggaccatt gaggtcccgc attcaggaca gaacttcgta
     3601 cctgccgggg aagccgtacc gcatttaggg c
//
LOCUS       DALTZZ010000052         1940 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_7711,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000052 DALTZZ010000000
VERSION     DALTZZ010000052.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7711"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /locus_tag="O0X12_00910"
     CDS             <1..1240
                     /locus_tag="O0X12_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HJK52754.1"
                     /translation="NPVLLKPKGNAVSQVVLLGKPWKDAKISDYYLETEYLLNEAVAA
                     YDRLKAEYTNIIVEGAGGAAEVNLYDRDIANIQLAKRLKIPIILVADIERGGVFAQVY
                     GTIALLPDDVRPLVKGIVINKFRGDPELFASGKTILEELTGVRVLGVVPWTQLNIPEE
                     DSLSLGDKTPAVREVRIAVIRYPHIANFTDVSLLEAAAAVEYVKPGTELDGYDAVILP
                     GTKNTVSDLADLKASGAADQILSYAKTNRPVIGICGGYQMMGRSIFDNAIEGDTVAEY
                     EGLGLLDVTTRFDHYDKVTRQVKRECTGCGPILARVKGAAGYEIHSGLSTVTGATVFT
                     NEGAADANGLIFGTYMHGLFANAGMVDALVSYLAEQKGVSYTPVREAGDPYAVLARHL
                     ESCLDVERIVELATVNYIRE"
     gene            1261..1611
                     /locus_tag="O0X12_00915"
     CDS             1261..1611
                     /locus_tag="O0X12_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein E"
                     /protein_id="HJK52755.1"
                     /translation="MALRLSFTLDPELSERIDLFAKKQDLDRNEAVLRLIESGLFVAE
                     QKGEMPPAPKRDFKEMVRMQRNVDLLLRSMDELKKEVRVMHHMINLEEKREAEEKAAV
                     KPVTKWLDRLDFTK"
     gene            complement(1650..>1940)
                     /locus_tag="O0X12_00920"
     CDS             complement(1650..>1940)
                     /locus_tag="O0X12_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HJK52756.1"
                     /translation="VMNLLQRVGMVSRANGGWMSGKVGGPVVVARRGGQTATLQEMLM
                     MYFICGMTVPGSTYWNIAFGQAPGTVAEDEEGVATMRTFAENVAFLIQKIRG"
BASE COUNT          451 a          493 c          603 g          393 t
ORIGIN      
        1 gaacccagtt cttctcaagc cgaagggaaa cgcggtttcg caggtggtgc tgctgggcaa
       61 accctggaag gatgcaaaga tcagtgacta ctatctggag acggagtatc tgttaaacga
      121 ggcggtcgcc gcctatgacc ggctgaaggc cgagtacacc aacattattg tggagggtgc
      181 gggtggagct gccgaggtga acctctacga ccgcgatatt gcaaacatcc agcttgcaaa
      241 acggctgaag atcccgatta ttctggttgc ggatatcgaa cggggcggcg tgtttgcaca
      301 ggtgtacgga accattgccc tgctgcccga tgatgtcaga ccgcttgtaa aagggattgt
      361 gatcaataag ttccgcggcg atcccgagct gtttgcgtcc ggcaaaacaa ttcttgagga
      421 gctgaccggt gttcgggtgc tgggagtggt gccctggacg cagctcaaca tcccagagga
      481 ggactcgctg tcgcttggcg acaagacgcc cgcggtgcgg gaggtgcgga ttgcagtcat
      541 ccggtatccg cacattgcaa actttacgga cgtttccctg ctggaggctg cggcagcggt
      601 tgagtacgtt aaaccgggaa cggagcttga cggctatgac gcggtgattc tgccaggtac
      661 aaagaacacc gtgagcgatc ttgcggatct gaaagcgtcc ggagcggcgg atcagatcct
      721 ttcctatgca aagacgaacc ggccggtgat cggtatctgc ggcgggtatc agatgatggg
      781 cagatccatc tttgacaatg ctatcgaagg ggacaccgtc gcagagtacg aagggctcgg
      841 cctgctggat gtgacaacgc ggtttgacca ctacgacaag gtcacgcggc aggtgaagcg
      901 ggagtgtacc ggttgcggcc cgattcttgc acgggtcaaa ggtgcggcag ggtatgagat
      961 tcactccggc ctgagcaccg ttaccggtgc aacagtgttt accaatgagg gtgcggctga
     1021 tgccaacgga ctgatctttg gaacatacat gcacggcctg ttcgccaacg ccggaatggt
     1081 tgacgcactg gtcagttacc ttgcggagca gaaaggcgtt tcctacacgc cggtaagaga
     1141 ggcaggagat ccgtatgcgg tgcttgcccg gcacctggag agctgtctgg atgtggaacg
     1201 gattgtcgag cttgcaacgg ttaactacat cagggaatga cacctatcta aacagtagag
     1261 atggcactca gattatcatt cacgctcgat cccgagctct cggagcggat cgatctgttt
     1321 gcaaaaaaac aggacctcga ccgcaacgaa gcggtgctcc ggctgattga gtcagggctg
     1381 tttgtcgcag aacagaaagg ggagatgccg cctgcaccca aacgcgactt caaggagatg
     1441 gtgcggatgc agaggaacgt ggatctgctt ctgcgcagca tggacgagct gaaaaaggag
     1501 gtgcgggtga tgcaccatat gatcaatctg gaagagaaac gcgaggccga ggagaaggcc
     1561 gcggtaaaac cggtcaccaa gtggctggac cggctggact ttaccaaata gacgtgtttc
     1621 gtctggaaaa aaacattttt ttcgcggggt tatccgcgaa ttttctgaat caggaacgca
     1681 acgttttctg caaacgtccg catggttgca acgccttcct catcctcggc aacggtgccg
     1741 ggtgcctgac cgaaggcgat gttccagtag gtggagcccg gaacggtcat accgcagatg
     1801 aagtacatca tcagcatctc ctgaagtgtt gccgtctggc cgccgcgccg tgccactaca
     1861 accggaccgc cgaccttgcc ggacatccag ccgccgtttg cacgggagac cataccaaca
     1921 cgctggagca ggttcatcac
//
LOCUS       DALTZZ010000053         2335 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_7720,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000053 DALTZZ010000000
VERSION     DALTZZ010000053.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7720"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(851..>2335)
                     /locus_tag="O0X12_00925"
     CDS             complement(851..>2335)
                     /locus_tag="O0X12_00925"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HJK52757.1"
                     /translation="ADEAVAIIKSVPPHCSSRRIPVVDAVGHRLSASLFAQYSVPEVP
                     VSAMDGFAVRSSETVGAGDQTPVVLRVFSRVNTGNVIPGEFDAVVRVEDAWFEGEDPH
                     EITVRKSVNPGTNVRPAGEDIKKGQLILPAGTVIRPFDVGAIAAYGITHVNVSRVSVG
                     ILPTGTELIEPGTKPRPGQVVESNTVMAEAYLRQFGVDVVRYPPVVDDPILIRGALER
                     VLAENDVVIVSAGSSAGTKDFTASVIEEMGKLLFHGVAMKPAKPAMFGVIEGKPVFGL
                     PGYPLSAQTVLRLFVRELLENWGWTGPAQEFVQVKLGDSISSEGGIDEFSLHAAARLG
                     DRYVVIPQSRGASVQMTGVRSNVVIRIPFGVEGFEAGEEAPALLTVPREELERTVLIA
                     GVYDPVLDVLAETAMSANVRVRCGNFSGLSGLLLLMKNCCHAVCITDEADLGVLGDLK
                     VLVRPLGQNSERSAVRIVFREEMENDPLMQKLFLVMDAVNSGSN"
BASE COUNT          656 a          666 c          501 g          512 t
ORIGIN      
        1 ccaccatact atgataacat gatacgtgat agcacacggc attattttta aatacttgca
       61 tctataaaat tgtaattgga tttggaatga acgcagggtg acgaccttca catcacgtca
      121 gtcagacctg gttcatcatc tttttcttcg ttcactttga ctaaggaggc cgtctgcgtg
      181 ttcatatgac ggtactgcaa ctgtttttac ggattcgtga cgcgccaaga acaaatcccc
      241 atgaataacg aatgccatag tatgtttgga attagcgacc cgtgggtgtg gatgacggtt
      301 atacaacact agtgcttgaa atggggatta gtattcttgt acagccgcag ctcgcggttc
      361 ggtttatgac ggctaaggat gggaaatcac taaatcgtgt agtaccgatg gggggcgctg
      421 tttattgtga tgatgacggg cgttgccttt acggtaggac cgctgactaa tgcgaatttt
      481 atggagacaa acgagcagct tgctcttgtg gacgatgttt gtccatacag cagaatcaca
      541 ggtgcttggt atctgcaatg ctctatttgg gaggtgaatt atctggttgc acatttgtgg
      601 aatcatctgg acccttggtg gtgggaacag cactttcgat gatcgtgctg ttgatactaa
      661 ttcctcaaga taagaatcgg gtgacaccga aacaacagag aagttttgtg cgagagacac
      721 actaaaggcg aaaaaccaga taacctagaa aaaaaaccac tgctgtaaac taccacaaaa
      781 acaaacaaaa aagagcagaa aaagagatag cagggaagac cggctacaca cccattttat
      841 tcaacattac ttagttagac ccactattaa ctgcgtccat caccaagaac agcttctgca
      901 tcagaggatc attttccatt tcttcgcgga aaacgatcct taccgctgat ctctctgaat
      961 tctggcccaa cggacgaacc agtaccttca gatctccaag aacaccaaga tctgcctcat
     1021 ccgtaataca tacggcatga cagcagttct tcatcagaag cagcagacct gacaacccac
     1081 tgaagttccc acacctgacc cgaacattgg cagacatcgc ggtctccgcc aaaacatcca
     1141 acaccggatc atacactccg gcaatcagta ctgttctctc aagctcctcc cgcggcacgg
     1201 taaggagagc aggagcctcc tctcctgcct caaaaccctc aaccccgaat ggaatccgaa
     1261 tcaccacgtt cgaccgaaca ccagtcatct gtacgctcgc gccgcgggac tgcgggatca
     1321 ctacataccg gtctccgagc cttgccgccg catgcagact aaactcatca atcccaccct
     1381 cagaagagat agagtctccg agcttcacct gcacaaactc ctgagcaggt cctgtccatc
     1441 cccagttctc cagaagctca cgtacaaaca gacgcagaac agtctgtgcc gaaagcggat
     1501 atcctggcag gccaaacacc ggctttcctt caataactcc gaacatcgca ggctttgccg
     1561 gcttcattgc aactccgtgg aacaacaact tccccatctc ctcaatcacc gacgcagtga
     1621 aatcctttgt ccccgcagaa gaacccgcag agacgattac aacatcattc tccgcaagaa
     1681 cacgctccaa ggccccgcgg atcagtatgg gatcatccac tactggcgga taccgcacaa
     1741 catctacccc gaactggcgg agatatgcct ctgccatgac cgtattactc tccaccacct
     1801 gacccggacg cggcttggtc cccggctcaa tcagctcagt gccggtcggt agaataccaa
     1861 cagaaacacg ggatacattc acgtgcgtaa ttccataggc tgcaatcgca cccacatcaa
     1921 acggccggat aactgttcct gcaggaagaa tcaactgccc ttttttaata tcctcaccag
     1981 caggcctgac atttgtaccc gggtttaccg atttgcgcac cgtaatctca tgcggatcct
     2041 ctccctcaaa ccaggcatcc tcaacacgaa cgactgcatc aaactccccg ggaatcacat
     2101 tacccgtatt cacccgcgaa aaaacacgga ggacaacagg cgtctgatcc ccagcaccaa
     2161 ccgtctctga tgaccgaact gcaaaaccgt ccattgcaga aaccggaact tcaggaaccg
     2221 aatactgtgc aaataacgac gcagaaagcc ggtgacctac cgcatccaca accggtatgc
     2281 ggcgggatga acagtgcggc ggcacactct taataattgc aacagcctca tcggc
//
LOCUS       DALTZZ010000054         1831 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_7930,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000054 DALTZZ010000000
VERSION     DALTZZ010000054.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7930"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            13..516
                     /gene="tsaA"
                     /locus_tag="O0X12_00930"
     CDS             13..516
                     /gene="tsaA"
                     /locus_tag="O0X12_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HJK52758.1"
                     /translation="MEPVTYYPVGIIHSPHTNLAGMPIQPPAAKSFTGTIEILPELAD
                     GLTGIEGFTRLILIYQLHKSEGFSLTATPFLDTTTHGVFATRVPRRPNQIGFSVVKLL
                     GRKGNVLEIGEVDILDGTPVLDIKPYVPPFDAYPQERYGWFEGKLGAIESARSDDRFT
                     GDCRNAQ"
     gene            611..820
                     /locus_tag="O0X12_00935"
     CDS             611..820
                     /locus_tag="O0X12_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HJK52759.1"
                     /translation="MKISARNQIKGKIVKLTEGAVNTEVILEIAGGQQIASIITKTSA
                     QNLGLAVGKEAYAIIKASEVMIGTE"
     gene            complement(867..>1831)
                     /locus_tag="O0X12_00940"
     CDS             complement(867..>1831)
                     /locus_tag="O0X12_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK52760.1"
                     /translation="LGPTGAGKTILLETIAGIHSPDKGQIFLDGADITNAPPEQRGIG
                     MVYQDYMLFPHMTVKENIGFGLRQRGVPKGERDTAAETMAKTLGIAHLLDRYPATLSG
                     GEQQRTAIARALVLNPRVLLLDEPLSALDTVTRDRMRREIKEIHRKTGVTILHITHHF
                     EDIYALADRVVVMKDGEVAQEGTPETILRRPASDFIANFTGMENLFSGTAVCNEDGTA
                     ELSVGSVKLFAATDLTGQVRIGIRPEDLILSTKPLDSSARNSLPGTVTGIAENGIYSK
                     ISVECGDEMVLTAALTRQSVERLKLHTGAEVWVTFKATAVHVFR"
BASE COUNT          455 a          472 c          414 g          490 t
ORIGIN      
        1 atatacttcc tcatggaacc tgttacctat tatcccgttg gtatcatcca ctcgccgcac
       61 acaaatcttg ctggcatgcc catccagccc cctgctgcaa aatcctttac cggtaccatt
      121 gaaatcctgc cagaacttgc tgacggactg accggtattg aaggattcac tcgtctgatt
      181 ttgatctatc agctacacaa aagcgaaggt ttctctttga ctgcaacgcc cttccttgac
      241 acgacaactc acggcgtttt tgcaacccgt gttcctcgcc gtccgaatca gatcggtttc
      301 tctgtggtga aacttcttgg gcgtaaggga aatgtcttgg agatcggtga agtggatata
      361 cttgacggaa cgcctgttct cgacatcaaa ccgtatgttc caccgtttga cgcatatccg
      421 caggaacgtt acggctggtt tgaaggaaaa cttggcgcga tagaatcggc acgctcagac
      481 gatcgtttta ctggagactg tcgcaatgcc caatgatatt tactcgaaaa ttcggtaaat
      541 gttgtaaaat aagttcaata aacgcagtgc gttatatgga atccctgcaa atatatgtgc
      601 tgaaacaact atgaagatca gcgcaagaaa ccagatcaaa ggaaagattg tgaaactcac
      661 tgagggtgct gtcaacaccg aagttatcct tgagattgct ggcggacagc agattgcctc
      721 catcatcacc aaaacctccg cacagaacct tggacttgca gtcggcaaag aagcctatgc
      781 aatcatcaag gcaagcgaag tcatgattgg cactgaataa attttccccc tcattttctt
      841 ttctttattt ccatcatctg ttctagtcac cggaaaacat gtactgcagt tgctttgaag
      901 gtgacccaga cctctgcacc agtatgaagt ttcaatcgtt ccacactttg tcgtgtaagt
      961 gctgcagtca acaccatctc atccccgcac tcaactgaaa tctttgagta gataccgttc
     1021 tctgcaatcc ccgtcactgt cccgggaagt gagtttcgtg ccgaagagtc cagtggttta
     1081 gttgagagaa tcagatcttc cggccggata ccaatccgta cctgcccagt aagatccgtt
     1141 gcggcaaaca gtttcacaga acccactgaa agctcagcgg tcccgtcttc attgcatacg
     1201 gcagttccgg aaaacaggtt ttccattccg gtgaagtttg caataaagtc tgatgctggt
     1261 ctgcgaagaa ttgtctccgg cgttccttct tgggcaacct cgccatcctt cattaccaca
     1321 acccggtcag caagtgcata gatatcctca aagtggtggg tgatatgcag aattgtcacg
     1381 ccagtttttc tatgaatctc tttaatctcc cgtcgcatcc tatctcgtgt gacggtgtcc
     1441 agagcggaaa gtggttcatc cagcagaagt acccgtggat tgagcaccaa tgcccgtgca
     1501 attgccgtcc gctgttgttc tcctccagaa agcgtagcag gatagcgatc cagtagatgg
     1561 gcgatgccaa gtgtttttgc cattgtttcg gctgcagtat cacgctctcc ttttggaact
     1621 ccgcgctgac gaaggccgaa tccgatgttt tccttaaccg tcatgtgggg aaaaagcatg
     1681 taatcctggt agaccatccc aatacctcgc tgttcaggcg gagcattggt gatgtcagct
     1741 ccgtccagaa atatctgtcc tttgtcagga gagtggattc ctgcaattgt ctccagaaga
     1801 atggtctttc ctgcgccggt cggtccaaga a
//
LOCUS       DALTZZ010000055         4908 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_8195,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000055 DALTZZ010000000
VERSION     DALTZZ010000055.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8195"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="O0X12_00945"
     CDS             complement(<1..573)
                     /locus_tag="O0X12_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
                     /protein_id="HJK52761.1"
                     /translation="MILVPVTYKGGVYRLDEVVDYIEDLGGYIVQRHNIANEVVLQVL
                     MPQEDIEGLKEFSRPLAGEVQTSPLVGTEIAVVIPSLEIHHLPHSACDVAEYLRSHGS
                     KSNILGMARGFGKRISQINDEERDLINEHDMAIYVLGNFASCIEKKFPKLRRGITVPI
                     ILTGGPSREQLEKQTDPPVEGYVGGLGRFMH"
     gene            complement(570..1151)
                     /locus_tag="O0X12_00950"
     CDS             complement(570..1151)
                     /locus_tag="O0X12_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HJK52762.1"
                     /translation="MAGLDYFAVESTEDGADNYNKIATTVLQDHNLLSIVEGLHLYID
                     PAYPLFVATGLIRPPRAAIRVSDVGNVLVQDGKATISVGDETYLAPMLAMLWKRLGHD
                     NVDQPDRFTVIISNVDIPKDIENWVVIDPAESLFKDLIYAIQVIAPEGFKVRRENYGS
                     DRFSYAASENTLPAEDVDRIIADRFKHMEEELR"
     gene            complement(1152..2405)
                     /locus_tag="O0X12_00955"
     CDS             complement(1152..2405)
                     /locus_tag="O0X12_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HJK52763.1"
                     /translation="MSEQPVRIAQITCGAEYSGIQNEITTAAETVGGQMFYPDVALKD
                     VKRAYKDFGLPVKSPDLKLAIARAQAIVEGRIEADGIFIASCFRCAEAAIVRNELRRY
                     IVENSRIPVVSYSFNERTGASTLLTRLEALTTIARRRSLMAREVQVGITMGVDSGSST
                     TKCVIMQDNEIIGTGWRPTTEVIKSADEVVALALEESGLKISDIEAIGTTGYGRFLVG
                     KHLNADLVQEELTVNSKGAVYLADAQKGFATVIDIGGMDNKAISVNDGIPGSFTMGGI
                     CAGASGRFLEMTSKRLGVDITELGALSMKSEGGEDVPMNSYCIVFGTQSLVNALAAGY
                     KREDVAAAACHSVAQQVYEQQLQEVDIKEPVIMVGGSSLIQGLVRAMGRMLNTEVIVP
                     HHSQYIGATGGALLASGFIDKKKEA"
     gene            complement(2402..2848)
                     /locus_tag="O0X12_00960"
     CDS             complement(2402..2848)
                     /locus_tag="O0X12_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HJK52764.1"
                     /translation="MAKVFIYPATSLILSDLVARFGHQPLGSAISIREHIQTPGFDSP
                     PIQMTSDDPQKGLKWAAVEVPSGIRGRMSLYGPLVEEAEAAIIIDEPDLSFGCMGCAR
                     TNELLAFMLREKDMPRLELRYPTSKEEGVTFVAAIKEFLKNLEVQQ"
     gene            complement(2854..3312)
                     /locus_tag="O0X12_00965"
     CDS             complement(2854..3312)
                     /locus_tag="O0X12_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 6 protein"
                     /protein_id="HJK52765.1"
                     /translation="MKPYVPQYVGTVTKYVFVDSPNTTPQDIATAAYEIAQGVMIKET
                     CFGCQITGSPEDVERIIAHLRKLDPYRIYVKDRGFPPGDARRCRADLGGARPGYLGHE
                     YEMGLVRYIAYGLQETDRMTPEELSAAAGREMPTPEALDIDILESLMKEE"
     gene            complement(3309..4865)
                     /locus_tag="O0X12_00970"
     CDS             complement(3309..4865)
                     /locus_tag="O0X12_00970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HJK52766.1"
                     /translation="MQIFLNGEAKDVPQGTTLSTLLPSHPAGCGVAILRPGVTASGDV
                     ETPRDTSHLRFTTTAGDIVVELLPGVVLPVPTGDVSDANLRVHWEDKYAVAFGPFRAD
                     FIPDHQSYRYDRGVLCLGCGGYDSGTSYLMFSRLQHMGDHGAASGGAILGTVISGLGI
                     MNRWRNGDRITKIEHVFTSVDKTNAVVTTDLSLEVEDGMQIFSEILINAEGYKEDHSA
                     IDTSCTKSVEHLLFALRNSEYLIDRTASTYIRDHTEGKLAVPMELQKPRREGTVTVRT
                     SGKSSGAIYIYTKDIPSNQHHTRTGTVVRGIELARFANVGTKLSVKVVPEQLDLRGLP
                     LGEAVARAKARGLRVLADNRDVDGRVVIDQNPATTLEVLKEGKVALSTVALEDVIDIT
                     LDYEHAPRTVDLFRRVTGLKIYAIGSMPFFYNIDDEMYLFKPKFASNVNIIPENVPKQ
                     PVPPYSLAVTNDARKARGMTGVRSVANEEYGPTGEPFEGTNVIGTVLDIDKLPLIKDG
                     STVFIREVKP"
BASE COUNT         1208 a         1299 c         1213 g         1188 t
ORIGIN      
        1 atgcatgaac ctcccaagac ctccgacata tccttccacc gggggatcgg tttgcttttc
       61 cagctgttca cgtgacggac cgccggtcaa aatgattggc acggtaattc cgcggcgaag
      121 tttcgggaac ttcttctcaa tacaggatgc aaagttgcca agtacataga tggccatgtc
      181 atgctcgttg atcaggtcac gttcttcgtc attgatctgt gagatgcgtt tgccaaaccc
      241 tctggccatg ccgaggatgt ttgactttga accgtgacta cgcaggtact cggcaacatc
      301 acaggcagag tgcggcaggt ggtgaatctc aagacttgga atcacgaccg ctatctcggt
      361 cccaaccagc ggtgaggtct gcacctctcc tgcgagcgga cgggagaatt ccttaagccc
      421 ttcaatatct tcctgcggca tcagtacctg cagcacaacc tcgttggcga tgttgtgccg
      481 ctgaacgatg tagcccccga gatcctcgat gtagtcaaca acctcatcaa gccggtacac
      541 acctcccttg taggtaaccg gaacaagaat catcgcagct cctcctccat gtgtttgaat
      601 ctgtcagcga taatccgatc aacatcctca gccgggagtg tattctcact tgccgcatag
      661 gagaaacgat cggacccgta attctcacgg cgaaccttga acccctccgg tgcaattacc
      721 tggattgcat agatcaaatc cttaaacaac gactccgcgg gatcaataac aacccagttt
      781 tcaatatctt tgggaatatc aacgttgctg atgataaccg tgaaccgatc aggctgatcc
      841 acgttgtcgt gaccaagacg cttccacagc atggcaagca ttggagcaag ataggtctca
      901 tcaccaacgg aaatcgttgc ctttccatcc tgcacaagaa cattccctac atccgaaact
      961 ctgatggccg cacgcggtgg acggatcaga ccggttgcga cgaacaacgg atacgcagga
     1021 tcgatataga gatgcagacc ctcaacgatc gagagcaggt tatgatcctg cagaacggtt
     1081 gttgcgattt tattgtagtt gtcggcacca tcctccgtgg attcaaccgc gaagtagtct
     1141 aaacctgcca tctatgcctc ctttttctta tcaatgaaac cggatgctaa aagggctccg
     1201 ccggtcgcac caatgtactg cgagtgatgc ggaacaataa cctcagtgtt aagcatgcga
     1261 cccatggcac ggacaagtcc ctgaatcagg gatgaaccgc caaccataat gaccggctct
     1321 ttgatgtcga cctcctgaag ctgttgttca tacacctgct gcgcaacact atggcaggcc
     1381 gctgcggcga catcctcccg tttgtatcct gcggcgagtg cattcaccaa agactgggtt
     1441 ccaaacacaa tgcagtaact gttcatcggc acatcctcac cgccttcact cttcatggaa
     1501 agggcaccaa gctcagtgat atcaacacca aggcgcttgg aggtcatctc caggaatcta
     1561 ccggaagctc ctgcacagat accacccatt gtgaacgaac ccggaatacc atcgttgaca
     1621 ctgattgcct tgttgtccat accaccgata tcaataaccg ttgcaaaacc tttctgggca
     1681 tcggcaagat aaacggctcc tttggagttt accgtcagtt cttcctgtac aagatcagcg
     1741 ttcaggtgtt taccaacgag gaaacgacca taacccgtag taccgattgc ttcaatgtca
     1801 gagatcttga gaccggactc ctcaagtgcc agagcaacaa cctcgtccgc acttttgata
     1861 acctcagtag ttggtctcca gccggtaccg atgatctcat tgtcctgcat aatgacacat
     1921 ttggtagtcg aagatccgga atcaacaccc atcgtgatac cgacctgtac ctcacgcgcc
     1981 ataaggctgc gtctgcgggc gatggtcgtc agagcttcaa gtctggtaag aagagtggac
     2041 gcaccggtcc gttcattgaa tgagtatgac acaaccggga tgcgggagtt ttccacgatg
     2101 tatcgccgga gttcatttct gacaatcgca gcttctgcac agcggaaaca gcttgcgatg
     2161 aagataccat ccgcttcgat acggccttca acgatagcct gcgcacgggc aatagcaagt
     2221 ttcagatcag ggctttttac cggcaaacca aagtctttgt aggcacgttt gacgtccttt
     2281 aatgcaacgt cgggataaaa catctgtccg ccaacggttt ctgcagcagt agtaatctcg
     2341 ttttgaatac cgctgtactc tgcaccgcag gttatctgtg caattctgac aggctgctcg
     2401 ctcattgttg tacctccaga tttttcagga attctttgat tgcggcaaca aacgtgactc
     2461 cctcctcctt cgaggttgga taacggagct caagccgcgg catatccttt tcgcggagca
     2521 tgaaggcaag aagttcgttc gttcgtgcac agcccatgca gccaaaggaa aggtcaggct
     2581 catcaatgat gatcgcagct tctgcctcct ctacaagagg accatacagt gacatgcgtc
     2641 cccgaatacc ggacggcact tcaacggctg cccacttgag acctttctgc ggatcatcgg
     2701 atgtcatctg aatcgggggg gagtcaaatc caggtgtctg aatatgttca cgaattgaaa
     2761 ttgccgaacc gagcggctga tggccgaagc gtgccacaag atcggataag ataaggctgg
     2821 ttgccggata gatgaatact tttgccatgg tgtttactcc tccttcatga gagattccag
     2881 aatatcaata tcaagcgctt ccggtgtcgg catctcccga ccggctgctg cgctgagttc
     2941 ttcgggggtc atacgatcgg tttcctgcaa tccgtaggca atgtaacgca caagccccat
     3001 ctcatattca tgcccgagat aaccgggacg ggcacctccg agatctgcac ggcatctgcg
     3061 tgcatctccc ggcggaaaac cgcggtcctt cacatagatg cgatagggat cgagctttct
     3121 gaggtgagcg atgattcgct ccacatcctc agggctgccg gtgatctggc acccaaaaca
     3181 ggtctccttg atcatgactc cctgagcaat ttcatatgct gcggttgcaa tatcctgcgg
     3241 agtggtgttg ggagagtcaa cgaatacata cttggtaacc gttccaacat actgcggcac
     3301 atagggtttc atggcttcac ctcgcggata aagacggttg aaccgtcttt gataagcgga
     3361 agtttgtcga tgtcaagaac agttccgatg acattggttc cctcaaacgg ctcaccagtc
     3421 ggtccgtact cttcgtttgc aacgctacgt acacccgtca taccgcgggc ttttctggcg
     3481 tcattggtaa ccgcaagtga gtagggagga accggttgtt tgggaacgtt ctcaggaatg
     3541 atattgacgt ttgaagcaaa cttcggtttg aaaaggtaca tctcatcatc aatattatag
     3601 aaaaacggca tgctgccgat tgcatatatt ttgaggccgg taacacggcg gaagagatca
     3661 accgtccggg gagcatgctc ataatccagt gtgatgtcga tgacatcctc aagggcaacg
     3721 gttgaaaggg caacttttcc ttctttgagg acctcaaggg ttgttgcagg attctgatcg
     3781 ataacaaccc gtccatcaac gtcacggtta tcggcaagta cacgaagtcc ccgtgccttt
     3841 gcacgggcaa ctgcttcccc taacgggaga ccacggagat caagctgttc cggcacaact
     3901 ttgacggaca gtttggttcc gacgttggca aaccgtgcaa gttctatacc acggacaacg
     3961 gttccggtac gggtgtggtg ctggttgctt ggaatgtctt ttgtatagat gtagatggcg
     4021 ccagagcttt ttcctgaagt tcgtactgtg acagttcctt cacgtctggg tttttgcagt
     4081 tccattggga cagcaagttt cccctccgtg tgatcacgga tgtaggtgga tgccgtccga
     4141 tcaatgagat attcagagtt ccgcaatgca aacagaagat gttccactga tttggtacag
     4201 gacgtatcaa tcgcactgtg atcctctttg tagccttctg cattgatcag aatctcggaa
     4261 aatatctgca taccgtcctc gacttcgagg gagagatcag tggttacgac agcattggtt
     4321 ttgtcaaccg aggtgaaaac gtgttctatc tttgtaatac gatcgccgtt tctccaccga
     4381 ttcatgatcc caagacctga gataacggtt ccaaggatag ctcctccaga agctgccccg
     4441 tgatctccca tgtgctgaag gcgggagaac atcagatagg aagtcccaga gtcataaccg
     4501 ccgcatccaa gacagaggac gccgcgatca tagcggtatg attggtgatc gggaatgaaa
     4561 tcagccctga aaggaccaaa agcaacggcg tatttgtctt cccagtgaac ccggagattt
     4621 gcatcggaaa catccccggt cggaacggga agaactacac ctggcaagag ctcaacgacg
     4681 atatcacccg ccgttgtggt aaaccggagg tgcgaggtgt cgcgtggcgt ttccacatct
     4741 ccggatgcag tgacaccggg tctgaggatg gcaacaccac atcctgccgg atgggaggga
     4801 agaagggtag aaagcgtggt tccctgcgga acatccttcg cctccccatt gaggaatatc
     4861 tgcatgtttg tcacaataac tctctttttc agattttaca tctcaaac
//
LOCUS       DALTZZ010000056         4216 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_8464,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000056 DALTZZ010000000
VERSION     DALTZZ010000056.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8464"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="O0X12_00975"
     CDS             <1..801
                     /locus_tag="O0X12_00975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJK52767.1"
                     /translation="GGVSSDGTDQICQEFATAEPNRFRFLIQTGNGVSNARNDGILAA
                     RGKYVAFLDADDLWVPEYLDMMAHLIRDFPEAIFYVSGFSRLYADKTVINCPKPPLRG
                     YIDYFQHTPWVHTSALIVERETIVRIGLFKTEYPLGEDIDLMIRLALHGNAAYEPTIL
                     EVHKIGVPGSLCTSGTKNDYLPVPGNSELFAAERTPSLILFHDKQILGTAMANLDRGY
                     PNEARKQLKGVSNWSTKLLIYRIASYLPTSAFLQIHSWYNRNVWKRKI"
     gene            783..1688
                     /locus_tag="O0X12_00980"
     CDS             783..1688
                     /locus_tag="O0X12_00980"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HJK52768.1"
                     /translation="MEEENMKNQKFIYQTKKIQVLLHQAIQELHHYPMNHTITYAGFF
                     GVKNWGDAINSILLQQLSGKTPIISPLGKQSNRCLLKTSEIIYSFIGSTLELASKKKN
                     LIVYGTGFMNSNDRIIQKPKKICAVRGPLTAERIIESIEDCPKIYGDPAVLFKRYYQP
                     KADKKYNLGIVPHWSDYQKISHCYANIPGVHVINAESGSTQFIDEICSCRCIASSSLH
                     GLIMADTYDIPTVWIKMISGLHPDDFKFYDYYGSIRCEREPINLFSWNIDDICSGTDL
                     ISHPINSEKLLMACPFLQPHTEQSR"
     gene            complement(1759..2514)
                     /locus_tag="O0X12_00985"
     CDS             complement(1759..2514)
                     /locus_tag="O0X12_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52769.1"
                     /translation="MFFIMRDLITGTISAFVKAYERRPGTATQWGDPLVGFCDANSDL
                     VQGLKQSVSPDHLMPSDLLPDATVVVVYFLPFTRELAETNRSGTAASPDWARAYAETN
                     ALFAELNDHLADLIQSHGGRAALPGTAAGFSGTLLKSRWSHRHFAVLAGLGTFGMNNM
                     LITSAGCCGRVSSFAADIPVTLDKPLSEEFCLYKRSGKCGRCFSRCPSGALSPEGFDR
                     ERCFAVCMENEARYPGCDVCGKCVTGVPCSFWE"
     gene            complement(2586..3863)
                     /gene="thiC"
                     /locus_tag="O0X12_00990"
     CDS             complement(2586..3863)
                     /gene="thiC"
                     /locus_tag="O0X12_00990"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HJK52770.1"
                     /translation="MTIVEDAKRGLVTEEMKVVAAAEGVTEDFIRRGIAGGHIVIPMS
                     PYRKVKLCGIGSGLRTKVNASIGTSSDIVNVEEEIEKARQAELAGADSLMELSTGGDF
                     LDIRRRVIEATTLSVGSVPLYQAFIEAARKKGGVVFMDEDDLFKITEEQAKLGTNFMA
                     IHTGINYETVKRLKHQGRHGGLVSRGGAFMTAWMLHNEQENPLYRRFDYLVEILKEHE
                     VTLSFGNGMRAGACHDATDRAAIQELLINAELADQAHAAGVQCIMEGPGHIPLDEIAA
                     NVQLEKRVTNNKPFYMLGPLVTDIAPGYDDRVAAIGASVSSAAGADFICYVTPAEHLA
                     LPTPEEVYEGVISSRIAAHVGDMVKLPKTREADLEMGHARRDLDWERQYAVSLNPKKA
                     REIRDSRMPADTDACTMCGDYCAIKIVQKNFHF"
BASE COUNT         1268 a         1074 c          873 g         1001 t
ORIGIN      
        1 ggtggtgtgt cctcagacgg aactgatcag atctgccaag aatttgcgac ggcagaacca
       61 aaccggtttc gatttctgat acaaacagga aatggggttt caaatgcaag aaatgatggg
      121 attcttgcag cgcgtggaaa gtatgtggcg tttctggatg ctgatgatct ctgggtgcca
      181 gagtaccttg acatgatggc acatctcatc agggattttc ctgaagctat tttttacgta
      241 agtggctttt cccgactata tgcagataaa acagtaataa attgcccaaa acctccactt
      301 agaggatata tcgattattt tcaacataca ccatgggtac atacgtctgc attaatagtg
      361 gagagagaaa ctattgttag aattggcctt tttaaaacgg aatatcctct tggagaagat
      421 attgacctaa tgatccgatt agcacttcat ggcaacgctg catatgaacc aacaatactt
      481 gaagtacata aaattggagt cccaggatcc ttatgtacaa gtggaacaaa gaatgattat
      541 cttcccgtac caggaaattc tgaattattt gcagccgaac gaaccccatc cctaattctc
      601 ttccatgaca agcaaatact cggaactgcc atggcaaatc tagatcgcgg ttacccaaac
      661 gaagccagaa aacagctaaa aggggtgagt aactggtcaa caaaactact gatataccga
      721 atcgcgtcat atctaccaac ctctgcattt ctacagattc actcatggta caacaggaat
      781 gtatggaaga ggaaaatatg aaaaatcaga aattcatcta tcagaccaaa aaaatacaag
      841 ttcttctcca tcaggcgatc caagaattac accattatcc tatgaatcat accatcacat
      901 atgcaggatt ttttggtgtt aaaaactggg gtgatgcaat aaattctata ctattgcaac
      961 aactttccgg aaaaactccc atcatctcac cattagggaa acaatcaaac agatgtctac
     1021 taaaaacatc agagataatc tattcattca ttggtagtac tctcgagctt gcctctaaaa
     1081 agaaaaatct gattgtgtat ggaactggat ttatgaactc caatgacagg ataatacaga
     1141 agccaaaaaa gatctgtgct gtccgagggc cacttacagc ggaacgcatt atagaaagca
     1201 ttgaagactg cccgaaaatt tacggtgatc cagcagtgct ttttaaaaga tattatcaac
     1261 caaaggcaga taaaaagtac aaccttggta tagtaccgca ttggagcgat tatcagaaaa
     1321 tttcccattg ctatgcaaac atacctggag tacatgtgat caatgcagaa tctggctcta
     1381 cacaattcat agatgaaata tgttcatgca ggtgtattgc ttcaagctca cttcatggtc
     1441 ttatcatggc agatacctac gacattccaa cagtatggat caaaatgata tcaggcctac
     1501 atcctgatga tttcaaattt tatgattatt atggttccat acgttgtgag agagaaccta
     1561 tcaacctatt ctcatggaat atagatgaca tttgctcagg tacagaccta atctcccacc
     1621 cgataaacag tgaaaaatta ctcatggcat gtccatttct tcaacctcac acagaacaga
     1681 gtcgctaaat atgcatgaga atatttgaga gtacacccaa acaacgacct ctagaaaaac
     1741 aacaaattat gactacaatt attcccaaaa cgaacacggg acaccggtta cacacttccc
     1801 gcacacatca cagccgggat aacgcgcttc attttccata cacaccgcaa aacaccgttc
     1861 ccgatcaaaa ccctccggtg aaagtgctcc cgacggacaa cgggaaaaac atctgccgca
     1921 tttgccggag cgcttgtaca aacagaactc ttcagaaaga ggtttatcca aagttaccgg
     1981 aatatctgcc gcaaacgaac tcactctgcc gcagcagccg gctgatgtga tcagcatatt
     2041 gttcatacca aatgtcccaa gacctgcaag gacggcaaaa tggcggtgcg accaccggct
     2101 tttcagaaga gtaccggaaa acccggcagc cgtgcccggc aaagcagctc tgcctccgtg
     2161 agactgaata agatccgcaa ggtgatcgtt taactccgca aacagtgcat tcgtctcggc
     2221 atacgcccgt gcccagtcag gagaagcagc ggtaccggat cggttcgtct cggcaagttc
     2281 acgggtaaac ggcaggaagt acacaaccac cacagttgca tccggaagaa gatccgacgg
     2341 catcagatga tcaggggaaa cactctgttt caacccctgc acaagatccg agttcgcatc
     2401 gcagaatccc accagcggat ctccccactg cgttgcagtg ccgggccgcc tctcgtatgc
     2461 tttcacaaac gctgaaatcg ttccggtgat cagatcccgc atgatgaaaa acatacagcg
     2521 gatagaaaaa taagcatacc ggaacaaaac agagaaggaa ctactgcaaa agaatataaa
     2581 aaagattaga agtggaagtt cttctgcaca atcttaattg cacagtagtc accgcacatt
     2641 gtacacgcat ccgtgtccgc aggcatccgt gagtcacgga tctcccgtgc cttctttgga
     2701 ttcagcgaaa ccgcatactg gcgttcccag tcaagatcgc gtcttgcatg acccatctcc
     2761 agatctgcct cacgggtctt tggaagcttc accatatcac ccacgtgggc agcaatccgg
     2821 gagctgatca ctccttcgta cacctcttcg ggagtcggca aggcaaggtg ctctgcaggc
     2881 gtcacataac agataaagtc agcaccagcc gcagaagaga ccgaagcacc aatggccgca
     2941 acgcgatcat cgtatcccgg agcaatgtct gttaccagcg gaccaagcat atagaacggc
     3001 ttgttgtttg tcacccgctt ctccagctgc acgtttgccg caatctcatc cagcggaatg
     3061 tgaccaggac cttccataat acactgaaca cccgctgcat gtgcctgatc tgcaagctct
     3121 gcgttgatca aaagctcctg aattgccgca cgatcggttg catcatggca ggcaccggct
     3181 ctcataccgt ttccaaacga gagtgtaacc tcatgctcct ttaaaatctc aacgagataa
     3241 tcaaacctgc ggtacagcgg attttcctgc tcgttgtgaa gcatccatgc cgtcataaac
     3301 gcaccgccgc gggagacaag accgccgtgg cgtccctggt gcttcagacg cttcaccgtc
     3361 tcatagttga tacccgtgtg aattgccata aagttcgtac cgagctttgc ctgctcctca
     3421 gtgatcttaa acagatcatc ctcatccata aagaccacac ctcccttctt gcgtgcggcc
     3481 tcaataaatg cctgatacag aggaactgat cccacagaaa gcgttgttgc ctcaatcaca
     3541 cggcggcgga tatcaaggaa atcaccaccg gtcgaaagtt ccatcagcga gtctgcacct
     3601 gcaagctctg cctgacgtgc cttttcgatc tcctcttcca catttacaat atcagatgac
     3661 gtaccaatcg acgcgttcac cttcgttcgc agaccagacc cgataccgca cagctttacc
     3721 ttccggtacg ggctcatcgg aattacaata tgtccgccag caataccgcg acggataaaa
     3781 tcctcggtca cgccttcggc ggcagcgacc accttcatct cttcggtgac aagtccgcgt
     3841 tttgcgtctt ccacaatagt catggttagt aatcggaggg aaacgatata aactatttta
     3901 tttttgttaa ataaaataca atttaatttg tggagaagac aagcagagat gaactcaaaa
     3961 cagataattc ggagtaacca gagatctgct ccagaaaagt tgtattgaag agtgattttc
     4021 ttggtatgct gacaagcaga cttgcgttca gtgatccatc atacgttcaa aaaatctcgg
     4081 cggacgatga tcctcccgca catccacacg gacatatccc ggctccggtg catcaaatat
     4141 cgcaggaaca agaacctccg caagcttttg gcctgcaaat tctcctgcaa cagtaccgat
     4201 catggtcccg atgtac
//
LOCUS       DALTZZ010000057         1864 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_8651,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000057 DALTZZ010000000
VERSION     DALTZZ010000057.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8651"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /locus_tag="O0X12_00995"
     CDS             <1..588
                     /locus_tag="O0X12_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HJK52771.1"
                     /translation="HWTTSPEAAPDKPEIVSITFENGVPVALNGKKMNGVDLIVELNT
                     IAGRNGVGRNDMVEDRVLGLKARENYEHPAATVLIAAHADLEKLVLSRFELKFKHIVD
                     DQWAELAYMGLVHEPLYHDLNAFIDETQKRVTGTVDVQLFKGGLHILGRKSPNAIYSQ
                     DMVSFDTTTIDQRDAIGFSKYFGLQGRMVWQLQNK"
     gene            648..1586
                     /gene="aglJ"
                     /locus_tag="O0X12_01000"
     CDS             648..1586
                     /gene="aglJ"
                     /locus_tag="O0X12_01000"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer glycoprotein N-glycosyltransferase AglJ"
                     /protein_id="HJK52772.1"
                     /translation="MDFSKDDVCVLIPTLNEKATIGPVIEELQKEGYHHILVVDGHST
                     DGTPEIAKKLGATVMTQEGKGKGAAMIEAFEKITSPYILMLDGDGTNPPEYADAMIEP
                     LVSGRADHVIGNRLNQYERGALTRLNLWGNRIMNTLFKWAHGVYMTDILSGYRAFTLE
                     SVKQMNLKEAGFEIETEISSAVVHQGLRFEVVPTFYKKRPGSPTKLHPIRDGYKILRA
                     INKYGKMNNPLFHFSVIGVLMGIAGFCLGLFVLFEWLAGIEHLFMGILTMLLIVAGIL
                     TFMIGFISDMILGYHREQMRELRMLREKLDEMKNKE"
     gene            1651..>1864
                     /locus_tag="O0X12_01005"
     CDS             1651..>1864
                     /locus_tag="O0X12_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52773.1"
                     /translation="MTTPRIGKVLHASIDKLYVGSEEFLAEIEAGLGASKISAPKVGY
                     QGVAGSFGEQAAREYFGETASTITNYK"
BASE COUNT          518 a          451 c          469 g          426 t
ORIGIN      
        1 cactggacca cctcaccgga agctgcgccc gacaagccgg agatcgtatc catcaccttt
       61 gaaaacggtg tcccggttgc cctgaacggc aaaaagatga atggtgtcga tttaatcgtt
      121 gaactgaaca cgattgccgg cagaaacgga gttggcagaa acgatatggt ggaagaccgt
      181 gtccttggac tcaaggcccg tgagaactac gaacacccgg cagcaacggt tctgattgcc
      241 gcacacgccg atcttgaaaa gctggttctt tcccgctttg agctcaagtt caagcacatt
      301 gtggatgacc agtgggcaga gctcgcctac atgggtcttg tacacgagcc actctaccac
      361 gatctgaacg catttattga cgagacgcag aaacgtgtca ccggaaccgt ggacgttcag
      421 ctgtttaagg gaggacttca cattctcggc agaaaatcac cgaatgccat ctactcacag
      481 gacatggttt cgtttgatac aacaaccatc gatcagcgtg atgcaatcgg cttctccaaa
      541 tacttcggac tccagggcag aatggtctgg cagctccaga acaaataatc ttttttcccg
      601 tacatctgta agactgatta cagagaacat ccaaagaata tcatagaatg gacttttcca
      661 aagatgacgt ctgtgtgtta atcccgacac tcaacgaaaa agccaccatt ggacctgtta
      721 tcgaggaact gcaaaaagag ggataccacc atatactcgt cgtggacggc cacagtacgg
      781 acggaacgcc ggagattgca aaaaaacttg gtgcaaccgt tatgacgcag gagggaaaag
      841 gaaaaggtgc ggccatgatc gaagcatttg aaaagatcac ctctccctat atcctgatgc
      901 ttgacgggga cggtaccaac ccgccggagt acgcagatgc catgatcgaa ccgcttgtaa
      961 gcggcagggc cgatcatgtc ataggtaacc ggcttaacca gtacgagcgt ggcgctctga
     1021 cccgcctgaa cctttggggg aaccggatca tgaacacact gtttaaatgg gctcatggtg
     1081 tgtatatgac agacattctt tccgggtatc gagcattcac cctggaatcg gtcaagcaga
     1141 tgaatctcaa ggaagcagga tttgagatcg agaccgagat cagttccgca gtagtccatc
     1201 agggtctcag gttcgaggtt gtgccgacat tctacaaaaa acgtccgggg tctccaacaa
     1261 aactgcaccc aatccgggac ggatacaaaa ttctccgtgc aattaacaaa tacggtaaaa
     1321 tgaacaatcc gctcttccat ttcagcgtca taggtgttct gatgggtatt gcaggattct
     1381 gcctcgggct ttttgtgcta tttgagtggc tggccgggat tgaacatctg tttatgggta
     1441 ttctgaccat gcttctcatt gttgcaggaa ttctcacctt catgatcgga ttcatcagtg
     1501 acatgatcct cggctatcac cgcgaacaga tgcgggagtt gcggatgctc cgggaaaaac
     1561 tcgacgaaat gaaaaataaa gagtgaactg tttttcgtga ggaagagaat cctcacattc
     1621 aaatttttgc gggttgatta tgtacatagt atgaccaccc cccggattgg aaaagtactc
     1681 catgcctcaa tagacaagct gtatgtcggc agcgaagagt ttctggcaga gatcgaagca
     1741 ggacttggtg cctcaaaaat ctctgcacca aaagtaggat atcagggtgt cgcaggttct
     1801 tttggagaac aggccgcccg cgagtacttc ggggaaactg catcgacgat caccaactac
     1861 aaag
//
LOCUS       DALTZZ010000058         1625 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_9122,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000058 DALTZZ010000000
VERSION     DALTZZ010000058.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9122"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="O0X12_01010"
     CDS             complement(<1..386)
                     /locus_tag="O0X12_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HJK52774.1"
                     /translation="MAKKAPYIVPVGPIHPALKEPVHIELTVVGEEVISADFTPGQTH
                     RGIEWMGLSRNPVQIVHLTDRICGICGVTHSLAFARAVEQIADITVPERADYVRTIIA
                     EFERIQSHLLWAGVAAHELGFDTLFYL"
     gene            complement(387..977)
                     /locus_tag="O0X12_01015"
     CDS             complement(387..977)
                     /locus_tag="O0X12_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HJK52775.1"
                     /translation="MMDEILPAETVVERLTAALGDAVLDSRIQKRAEGKEKRENTNIW
                     ITIRRDAVHAAVEALMDIWYPHLSCIAGYDKGADSPDLRVQYIFSIYGGVARSEYMVI
                     FSLDVPKDDARLPTITDLLEGTAFTEREKTEYLGITIDGLAPAAHKFFLPQDFPDNVY
                     PLRKDDKKIPDTMVKDLWACGRPAGRPPAPLDGGEE"
     gene            complement(974..1411)
                     /locus_tag="O0X12_01020"
     CDS             complement(974..1411)
                     /locus_tag="O0X12_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HJK52776.1"
                     /translation="MKLTTSLNRSLWVFHFNSGSCNGCDIEIVATLTPRYDPERFGVK
                     LVGSPRHADVLLVTGPVTNQKADRLRRVYAQMPGPKVVMVVGTCGQSGGVFSTSYNLA
                     GPVDQIIPVDVYVPGCAPRPEAIIQGVVTAIAKLERLEGGAKQ"
     gene            complement(1408..>1625)
                     /locus_tag="O0X12_01025"
     CDS             complement(1408..>1625)
                     /locus_tag="O0X12_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HJK52777.1"
                     /translation="DAETTAPYLSGNAEPEKGAVHIRAGNMYWGFTKALSAYYDRLIP
                     LHSGIVNDYVSWFLIGVVILFVVVILV"
BASE COUNT          357 a          466 c          437 g          365 t
ORIGIN      
        1 agatagaaca acgtatcaaa tccaagctcg tgtgctgcaa cacccgccca gagcaggtgg
       61 gactggatac gctcgaactc tgcaatgatc gttctgacat aatccgcccg ttccggaacc
      121 gtgatgtctg cgatctgttc caccgcccgg gcaaatgcaa gtgagtgagt cactccgcag
      181 ataccgcaga tacggtcggt cagatgcacg atctgcacgg gatttcgcga cagtcccatc
      241 cactcaatac cgcggtgcgt ctgtcctggg gtgaagtcag cagatatgac ctcttctccg
      301 accacggtca gttcgatatg gaccggctct ttgagtgccg gatgaatcgg gccgaccggt
      361 acaatatacg gagctttctt tgccatttac tcctcacctc cgtcaagcgg tgccggtggg
      421 cggcctgctg ggcggccaca tgcccagaga tctttcacca ttgtgtcagg gatctttttg
      481 tcgtcttttc ggagcggata cacattgtcc gggaagtcct gcggcaggaa gaacttgtgt
      541 gctgccgggg caagaccgtc aatggtgata ccgagatact cggttttttc ccgttccgta
      601 aatgcagtac cttccagcag atccgtgatc gttggaagac gggcgtcgtc ttttggaacg
      661 tcaagtgaga agatcaccat gtactctgat cttgcaacgc ctccgtagat ggagaagatg
      721 tactgcacac gcagatcagg cgagtcagcc cctttgtcat atccggcaat acaggagagg
      781 tgcggatacc agatatccat cagtgcttca accgcggcat gaaccgcatc tcgccggatg
      841 gttatccaga tgttggtgtt ctcccgcttc tcctttccct cagctctttt ctgaatgcgg
      901 gagtccagca ccgcatcacc aagcgctgct gtcaggcgtt caacaacagt ttccgccgga
      961 agaatttcgt ccatcattgt ttggcacctc cttcgagtct ttcaagtttt gcaatcgcgg
     1021 tcaccacacc ctggatgata gcctccggcc gcggggcaca gccgggcaca tagacgtcca
     1081 ccgggatgat ctgatcaacc ggtcctgcaa ggttatagga cgtgctaaag acaccgcccg
     1141 actgaccaca ggtacctacc accatgacca ctttgggacc aggcatctgg gcatacaccc
     1201 tccgcagacg gtcggccttc tggttggtaa ctggaccggt taccagcagg acatccgcat
     1261 gccgcgggga gccaacaagt ttgaccccga atcgttccgg atcataccgg ggcgtaaggg
     1321 ttgcgacaat ctcaatgtca cagccgttgc acgacccgct gttgaagtgg aacacccaga
     1381 gggaccggtt cagcgatgtt gtcagtttca tacgaggatc accaccacaa agagtattac
     1441 aacgccgatc aggaaccagg agacgtaatc gttcacaatt cctgagtgca gcggaatcag
     1501 gcggtcatag taggccgaca gtgccttggt aaatccccag tacatgttgc cggcacggat
     1561 gtggaccgca cctttctccg gttcggcgtt tccggacaga tacggggcgg tggtctcagc
     1621 atcct
//
LOCUS       DALTZZ010000059         2075 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_9419,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000059 DALTZZ010000000
VERSION     DALTZZ010000059.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9419"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /gene="thrS"
                     /locus_tag="O0X12_01030"
     CDS             complement(<1..1576)
                     /gene="thrS"
                     /locus_tag="O0X12_01030"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HJK52778.1"
                     /translation="MKVIYNDGKVGECPKEEELHVIRHTAAHVLAQAVKRLYPHASFA
                     YGPATERGFYYDVDLGDTKLSEADLPAIEQEMREIVKANLPIKPFILSRADALKLMEE
                     RHEPYKIEHIADLDENEPLSFYEQGEYIDMCVGPHLTYTKTLKAFKLTGLSGAYWKND
                     SKNKMLTRINGTAFATTKELEDFLKLQEEAERRDHRKIGKDMKLFMFEDVGPGFPFFL
                     PNGMIIKNELIEYWRELHTANGYQEISTPLIMNRCLWETSGHWDHYKENMYATRIDDE
                     DYCIKPMNCPGGVMVYANEPHSYRELPMRLAELGIVHRHELKGVLHGLFRVRCFTQDD
                     AHIFMRQDQIAEEISSVIRLIHQVYSKFGFEYTLELSTRPEDSMGSDEDWEAATNGLV
                     NALEQLNLPYTINEGDGAFYGPKIDFHLRDCLGRTWQCGTIQLDFQMPINFNLSYVDE
                     NDERPRPIMIHRVCFGSIERFIGILTEHFAGKFPVWLAPMQAKVLLVSKKSEAYATEI
                     YQMLKDAKIRCELDNRN"
     gene            complement(1769..1842)
                     /locus_tag="O0X12_01035"
     tRNA            complement(1769..1842)
                     /locus_tag="O0X12_01035"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1806..1808),aa:Lys,seq:ctt)
     gene            complement(1871..1944)
                     /locus_tag="O0X12_01040"
     tRNA            complement(1871..1944)
                     /locus_tag="O0X12_01040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1908..1910),aa:Lys,seq:ctt)
BASE COUNT          449 a          524 c          527 g          575 t
ORIGIN      
        1 cattgcggtt gtccagctcg cagcggatct ttgcatcttt cagcatctga tagatctcgg
       61 ttgcatatgc ctcgctcttc ttggagacca gcagaacctt tgcctgcatt ggtgcaagcc
      121 agaccgggaa ctttccggca aagtgttcgg tcaggatacc gataaaccgc tcgatcgatc
      181 cgaaacatac gcggtggatc atgatcgggc gcggccgttc gtcgttctca tccacatagg
      241 ataagttgaa gttgatcggc atctggaaat ccagctggat ggttccgcac tgccaggtac
      301 gtccaagaca gtcgcgcaga tggaagtcaa tcttcgggcc gtagaatgca ccgtcgcctt
      361 cgttgatcgt gtagggaaga ttcagctgct ccagtgcatt caccagaccg tttgttgctg
      421 cttcccagtc ctcatcactt cccatgctgt cctccgggcg ggtggagagc tcaagagtgt
      481 actcaaagcc gaacttggag tacacctggt gaatcagccg gattacggaa ctgatctcct
      541 cggcgatctg atcctggcgc atgaagatgt gggcatcatc ctgtgtgaaa cagcggaccc
      601 ggaagagacc gtgcagaacg cccttcagtt catggcggtg cacaattccc agctcagcaa
      661 gacgcatcgg cagttcgcgg tagctgtgcg gttcgtttgc atataccatc acaccgccgg
      721 ggcagttcat cggtttgata cagtagtcct catcatcgat gcgggtcgca tacatgttct
      781 ctttgtaatg atcccagtga ccgctggtct cccagagaca gcggttcatg atgagcggcg
      841 ttgagatctc ctggtatccg ttggccgtgt gcagctcgcg ccagtactcg atcagttcgt
      901 tcttgatgat cattccgttc ggcaggaaga acgggaatcc cggacccaca tcctcgaaca
      961 tgaagagctt catgtctttg ccgatcttgc ggtgatcacg gcgctctgcc tcttcctgca
     1021 gtttcaggaa atcctctagt tccttggtgg tggcaaaggc ggttccgttg attcttgtca
     1081 gcatcttgtt tttgctgtcg tttttccagt atgcaccgga aaggccggtg agtttgaatg
     1141 ctttcagggt ctttgtatag gtcaggtgag gaccgacgca catatcgatg tattcgccct
     1201 gctcatagaa actcagcggt tcattctcat caaggtctgc aatgtgttcg atcttgtatg
     1261 gttcatgccg ctcttccatg agttttaatg catctgcacg tgagaggatg aacggtttta
     1321 tcggcagatt tgccttgacg atctcccgca tctcctgttc aatggcagga aggtcggctt
     1381 ccgaaagttt cgtgtctccc agatcgacat catagtaaaa tcccctctcg gttgcgggtc
     1441 cgtaggcaaa cgatgcgtgg gggtagagac gtttcactgc ctgggcaagt acatgagccg
     1501 cggtgtggcg tataacatgc agctcttctt cttttgggca ctcgcccact tttccgtcat
     1561 tgtaaattac tttcatgcaa gtagttcctg ctgttttccg tttcagcgga tatgcaaatc
     1621 ctcttgtacg ctgcaaaggc gagttctgtg cagcccaaga taaccgtaca tgttttggtt
     1681 ttttacagga tatgtatttt ggtcacgcag gactctggca tattcctgtc tgcaccatcc
     1741 gacttatttc ttaaatctgc actaaaagcg ggcctaaagg gatttgaacc cctggcctac
     1801 ggattaagag tccgtcgcta ttcctgacta agctataggc ccagtaaaaa cattcaactc
     1861 acaccaaaag cgggcctaaa gggatttgaa cccctggcct acggattaag agtccgtcgc
     1921 tattcctgac taagctatag gccctttgtg atgtctgttg acctaattag attagtcata
     1981 tcaatattta tagatttctt cacgagggga ttttgggccc tgtgggtatc tacattaata
     2041 tccaaacaac acgaatattg gtacatggtt gagag
//
LOCUS       DALTZZ010000060         1854 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_9528,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000060 DALTZZ010000000
VERSION     DALTZZ010000060.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9528"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="O0X12_01045"
     CDS             <1..413
                     /locus_tag="O0X12_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833033.1"
                     /note="eIF-2G; forms a ternary complex with GTP and
                     initiator tRNA in the early steps of protein synthesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HJK52779.1"
                     /translation="MNKASHKVPVATPGGLSAIGTKLDPAITKSDTLVGQVAGAVGSL
                     PPVWDKLKFDMTLMDRVVGSASEQRIEPLRLKEPLMLSVGTAVTVGIVMAAKKNQAEV
                     ILKRPVCAELGSRIAISRQVGGRWRLIGMGILTE"
     gene            377..790
                     /locus_tag="O0X12_01050"
     CDS             377..790
                     /locus_tag="O0X12_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HJK52780.1"
                     /translation="MASDRHGHSDRVNVVLDTNALMMPAQFGVDLFEGLRELLGGYDA
                     LVPAEVVYELRGLAQGRGNNAAAARFGLTVAARCTVLPPLEEEVPVDDKVIRTAEMFN
                     AVVVTNDKKLKDRLLSLRIPVVVLRSRSRLELMGK"
     gene            811..1383
                     /locus_tag="O0X12_01055"
     CDS             811..1383
                     /locus_tag="O0X12_01055"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HJK52781.1"
                     /translation="MYYKLKLNDKVRVPPERMGEDLNTVILDVLQEQFEGSVDKEMGI
                     FIAVTGVDRVGDGEIIYSDGGVYYDVDFEAVVLRLTLQEIVEGIVVETTSFGAFISLG
                     PIDAMLHMSQITDEYIDYDEKNSRLVCKDTGRTIAVGDRVRARVVALSLNEREPRESK
                     IGLTMRQPGLGTLAWIEDERNREKAEKERK"
     gene            1383..1598
                     /locus_tag="O0X12_01060"
     CDS             1383..1598
                     /locus_tag="O0X12_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase, subunit E''"
                     /protein_id="HJK52782.1"
                     /translation="MAPKRTQKKLLQACRTCHKVLEADKTVCPECQGNALTTEWFGYL
                     VIIDSRHSDVAKKMNIEYNGRYALKVR"
BASE COUNT          446 a          430 c          541 g          437 t
ORIGIN      
        1 ccatgaacaa ggcatcccat aaagttccgg ttgcaacgcc gggaggcctg tctgccattg
       61 gtacaaagct tgacccggcg atcacaaaga gtgatacgct ggtgggtcag gttgccggtg
      121 cggtaggttc tcttccgccg gtctgggaca aactgaagtt tgacatgacc ttaatggacc
      181 gcgtcgtagg atccgcatca gaacagcgta ttgagccgct gcggttgaaa gaacccctga
      241 tgctgtcagt tggaacagct gttacagttg gtattgtcat ggcggcaaag aaaaaccagg
      301 cagaggttat cctcaagcgg cctgtttgtg cggagcttgg atcccgcatt gcaatcagcc
      361 ggcaggtcgg tggcagatgg cgtctgatcg gcatgggcat tctgaccgag taaacgtcgt
      421 tctggatacg aacgcgctga tgatgccagc tcagttcggt gtggatctgt ttgagggtct
      481 gcgcgagctt cttggtggct acgacgcact cgtgcctgct gaggtggtgt acgagctgcg
      541 cggtcttgca cagggacgtg ggaacaatgc ggcggcggca cggtttggtc taactgttgc
      601 ggcgcgttgc acagttcttc ccccattgga agaggaggtc cctgtggatg acaaagtgat
      661 ccgaaccgca gagatgttta atgctgttgt agtgaccaat gataaaaagc tgaaagaccg
      721 cctgctcagt ctccgcattc cggttgttgt actccggtca cgatcacgac ttgagctgat
      781 gggaaaataa ttcgacgagg agccaaggat atgtattata aattaaaatt aaacgataaa
      841 gtcagagtcc cgccagaacg catgggtgag gatctgaaca cagtaattct tgatgtactg
      901 caggagcagt tcgagggtag tgttgacaaa gagatgggta tcttcatcgc cgtcacgggt
      961 gttgaccgtg ttggtgacgg tgagatcatc tacagcgacg gcggtgtgta ctatgacgtg
     1021 gactttgaag ccgttgtact tcgcctgaca cttcaggaga tcgttgaggg tattgtggta
     1081 gagacgacaa gctttggcgc gttcatctcg cttggaccca ttgatgcaat gctgcacatg
     1141 agtcagatca ctgatgagta catcgattat gatgaaaaga actcacgtct tgtctgcaaa
     1201 gacacgggaa gaacaattgc ggttggcgat agagtgcgcg cccgtgtagt ggctctctcc
     1261 ctaaacgaac gtgagccgcg tgagtcaaag atcggactta ccatgcgtca gccaggtctt
     1321 ggaacccttg catggattga ggatgagcgc aaccgcgaaa aggcggagaa ggagcggaag
     1381 taatggcgcc gaagcggaca cagaaaaaac tcctgcaggc atgccgtacc tgccataagg
     1441 tcctggaagc cgataaaacg gtctgcccag agtgtcaggg taatgctctt accacagaat
     1501 ggtttgggta tctggtcatt attgactccc gtcactcgga tgttgcaaag aagatgaaca
     1561 tcgaatacaa cggccgttac gcgcttaagg ttcgataatg cttgttctcc cgtcggaaca
     1621 ccgggggctg ttcaaacaac cctttggtac cctttttcca gaattttctg atattgtccc
     1681 gcagatatcc ggacgaatcg tttgtacggt gggagatgtg gtaacacact cagccctcac
     1741 acgagacatt actcccgcga taggtgttat tgacggattt accatgcgct ctccctatct
     1801 tgcaatgccg gagattgcgc agcatgtact gcgggtcaaa aacccggcgg gaac
//
LOCUS       DALTZZ010000061         2722 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_9638,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000061 DALTZZ010000000
VERSION     DALTZZ010000061.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9638"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="O0X12_01065"
     CDS             <1..327
                     /locus_tag="O0X12_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HJK52783.1"
                     /translation="GYYGKYYVKAQHAKQLIRRDFARVLTECDLLAGPTMPNVAPKLG
                     EMTADPLRMYQTDILTVPVNIAGIPAISLPCGTAHGMPVGLQIMGRMFGEEALLNAAL
                     AFEEAA"
     gene            328..1761
                     /gene="gatB"
                     /locus_tag="O0X12_01070"
     CDS             328..1761
                     /gene="gatB"
                     /locus_tag="O0X12_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HJK52784.1"
                     /translation="MAEEEMKVIIGLEVHCQLDTKSKLFCGCSADFRADAPNTHVCPV
                     CLGLPGALPVLNKKAIEYALKVAKALNCTIPEEGEFCRKNYFYPDLVKAYQITMGDNP
                     LALGGYIEIEDDAGNPKTVNLTRIHVEEDPGRLVHMGNKERGHYTLVDYNRSGIPLIE
                     MVTEPELSSPKEARRLLNKLRATLEYLDVFDPEKEGSIRVDANISIQGHERVEIKNIS
                     SYKGVEKALTFEITRQKNLIRRGGTIVRETRHFQEGKGTTTSARSKETATDYRYFPEP
                     DLPVIRVADWVKDIVLPELPDARRDRFMQQYGIAVNHARTLTGDLRLAEFYESVADVG
                     PAIAASWIADILIGELNYRDMALGTVASHTSEFKDLIVLVRDKKITDKAGVDVLRVWL
                     DDLHAGTKKETPAQIVERLGLARESGESFRGIVEQILAANPQAVADHKAGKKGALNFI
                     VGQVMKETKGKSDPREIHAMIAELVGE"
     gene            1764..>2722
                     /locus_tag="O0X12_01075"
     CDS             1764..>2722
                     /locus_tag="O0X12_01075"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HJK52785.1"
                     /translation="MHLIIAEKNLVAERIAAFLAGKQKVHTKRDGAATEYTFGDTVVM
                     GLRGHVVELDFTKGYSNWRSEEHPPRSLINAGIEKHPTEKKIVALMQKHAKKADRITI
                     ATDYDTEGELIGMEAYELVRAVNQKATVDRARFSAITKDEITKAIADAKEIDFNLAAA
                     GETRQVIDLVWGASLTRFLSIAAHRGSDNILSVGRVQSPTLAMIVDREKEIEAFVPEK
                     YWMLSLTAKVAGEEIAARHVHGRFTVKAEADAAFAGTRDPVIVREVITGKKNDKAPTP
                     LDTTALIVGAGRLGLSAASAMSKAEDLYMRGFISYPRTDNTAYP"
BASE COUNT          706 a          792 c          708 g          516 t
ORIGIN      
        1 ggatactacg gaaaatacta tgttaaagcc cagcacgcaa aacagcttat ccgccgcgac
       61 tttgccaggg tactaacgga gtgcgacctc cttgccggac ccaccatgcc caacgttgcg
      121 ccaaaactcg gtgaaatgac cgcagacccg ctgcggatgt accagacgga tattcttacc
      181 gtcccggtca acattgcggg aatccctgca atctcactcc cctgcggaac cgcccacggc
      241 atgccggtcg gtctccagat catgggaaga atgtttggtg aagaggcact gctcaatgca
      301 gcactcgcgt ttgaggaggc ggcataaatg gctgaagaag agatgaaggt catcatcggc
      361 ctggaggtac actgtcagct ggataccaaa tcaaagctgt tctgcggatg ttccgcagac
      421 ttccgggcag acgcgcccaa cacccatgtc tgtccggtct gtctcggact gcccggagca
      481 ctgccggtgt taaacaaaaa agcaatcgag tacgccctca aagttgcaaa agcactcaac
      541 tgtaccattc cagaagaggg agagttctgc agaaagaact acttctatcc ggatcttgtc
      601 aaagcctacc agataacgat gggtgacaat ccgcttgcac tcggcggata cattgagatc
      661 gaagatgatg ccggaaaccc gaagactgtc aatctgaccc gcatccatgt cgaggaagac
      721 cccggacgtc ttgtccacat gggcaacaag gaacgcggac actacaccct cgttgactac
      781 aaccgttccg gtattccctt aatcgagatg gtcaccgagc cggaactctc ctcgcccaaa
      841 gaagcacgcc gtctcctgaa caaacttcgg gcaacgctgg agtacctcga tgtgtttgat
      901 ccggagaagg agggatccat tcgtgtggat gcaaacatct ccattcaggg acatgagcgt
      961 gttgagatca aaaacatctc ctcctacaaa ggtgttgaaa aggcactgac gtttgagatt
     1021 acccgtcaga agaacctcat ccgccgcggc ggaaccatcg tccgcgaaac ccgtcacttc
     1081 caggaaggaa aaggaaccac taccagcgca cggtcgaaag aaactgcgac cgactaccgc
     1141 tacttccccg aacccgacct tccggtcatc cgcgtcgcag actgggtcaa agatatcgtc
     1201 ctcccagaac tgccggatgc acgccgcgac cggtttatgc agcagtacgg tatcgccgtc
     1261 aaccatgcac gaacccttac cggcgacctg aggcttgccg agttctatga aagcgtcgca
     1321 gatgtgggtc ccgcaattgc cgcatcctgg attgccgaca ttctgatcgg tgagctgaac
     1381 taccgcgaca tggcgcttgg aaccgttgcc tcccacacat ccgagttcaa ggacctgatc
     1441 gtgcttgtcc gcgacaaaaa gatcaccgac aaagcaggtg ttgacgtcct ccgtgtctgg
     1501 ctggatgatc tccacgcagg aaccaagaaa gaaaccccgg cccagatcgt tgaacggctc
     1561 ggtcttgccc gtgaatccgg tgagtccttc cgcggcatcg tcgaacagat cttagccgcc
     1621 aacccgcagg cagttgccga tcacaaagca ggaaagaaag gagctctcaa cttcatcgtc
     1681 ggacaggtca tgaaagagac caaaggcaaa tctgatccgc gtgagattca cgccatgatt
     1741 gcagagcttg taggagagta atcatgcatc tcatcatcgc cgaaaagaac ctcgtcgcag
     1801 aacgcatcgc agcatttctg gcaggaaaac agaaagtgca cacaaaacgc gacggcgccg
     1861 caaccgagta cacctttggc gataccgtgg tgatgggtct tcgcggccat gtggtcgaac
     1921 ttgacttcac caaaggctac agtaactggc gaagcgagga gcacccgccg cgttccctca
     1981 ttaacgcagg aattgaaaaa cacccgacgg aaaaaaagat cgttgctctc atgcagaagc
     2041 atgcaaagaa agccgaccgc atcaccattg caaccgatta cgatactgaa ggagaactca
     2101 tcggcatgga ggcctatgaa ctcgtccggg cggtgaacca gaaggcaacg gttgaccgtg
     2161 cccggttctc ggcaatcaca aaggacgaga tcaccaaggc aattgctgac gcaaaagaga
     2221 tcgacttcaa ccttgcagca gctggtgaga cccgtcaggt cattgacctt gtctggggag
     2281 catccctgac ccggttcctc tccattgccg cacaccgcgg ttccgacaac attctctccg
     2341 tcggccgcgt gcagagcccg acgcttgcca tgatcgttga ccgggaaaaa gagatcgagg
     2401 catttgtacc ggagaagtac tggatgcttt ccctcaccgc aaaggttgcg ggcgaggaga
     2461 ttgcagcccg ccatgtgcat gggagattca ctgtcaaagc agaggctgat gcggcatttg
     2521 ccggaacacg cgatccggtc atcgtacggg aggtcatcac cggcaagaaa aacgacaagg
     2581 caccaacccc gcttgacacc actgccctta ttgtcggggc aggacgtctt ggtctctccg
     2641 ctgcgtccgc gatgagcaaa gcagaggatc tttacatgcg gggatttatc tcctatcccc
     2701 gtaccgataa caccgcatac cc
//
LOCUS       DALTZZ010000062         2532 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_9696,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000062 DALTZZ010000000
VERSION     DALTZZ010000062.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9696"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="O0X12_01080"
     CDS             <1..807
                     /locus_tag="O0X12_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HJK52786.1"
                     /translation="ALEYLVQSGMITEEADGSMWGTQFGVLASRLYLDPETASMLREM
                     LEKQETFSTFGMLHLLCMTPDMFRFYLKASDERLVDQVFCERGDELWCEELSEEFFAA
                     VKTALVAEAWCEEVPEEIICENFGVGAGDIHAVVENLRWLMHAAGRIAHEFAPRFDAD
                     VRDLEIRIENGVKAELLPLISLRGIGRIRARRLFNRGITTPEELLAADTRDVVAILGN
                     VLAKNVLEQAAKKTGKTMEIAELPEEKNETVPATASEEEKPQKTLFDFGE"
     gene            808..1317
                     /gene="cgi121"
                     /locus_tag="O0X12_01085"
     CDS             808..1317
                     /gene="cgi121"
                     /locus_tag="O0X12_01085"
                     /inference="COORDINATES: protein motif:HMM:NF011465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HJK52787.1"
                     /translation="MMTEQLLTIHEAKATVENTAAVLAQINKIAEKTGSTIVLFDAGK
                     VAGPDHIRSAVRHAERSFASGKPVARTLAMEILLYASGQRQCSLAPKFGLHTGENLLF
                     TVILGGDAAQAQQLLNGIVSFTMHGVTASRATLMEEFGITEDEIAVVGEDRMNELVIE
                     RVALMDAYK"
     gene            complement(1319..1825)
                     /locus_tag="O0X12_01090"
     CDS             complement(1319..1825)
                     /locus_tag="O0X12_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJK52788.1"
                     /translation="MPDLSFSSVKTDAEIVAVAELAQEIWTEHFPPIIGPAQTAYMIE
                     TFQSAKAIHRQITEDCYQYYLLQLGSDRIGYTAVHPEPKNHAMFLSKIYIRKKFRGSG
                     YSRTTMNFIADLCRDDGLNMIYLTVNKQNLSSIAVYEKLGFVRARELVTEIGNGFVMD
                     DYVMEKYL"
     gene            complement(1828..2316)
                     /locus_tag="O0X12_01095"
     CDS             complement(1828..2316)
                     /locus_tag="O0X12_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HJK52789.1"
                     /translation="MPAEHKKDISIQTVVITVSTTRTEKTDLSGKIIRDAFTAADIPV
                     VSVVIVKDDISEIRTAVLEALHVANCIVVNGGTGLTHDDCTIEAVAPLFQKTMDGVGE
                     LFRMKSYEQVGNSVILSRASAGITNGKAIFCIPGSPKAVKLATEEIIIPEIRHILTHA
                     GQ"
BASE COUNT          656 a          560 c          676 g          640 t
ORIGIN      
        1 gcactggagt atctggtaca aagcggaatg atcaccgaag aggctgacgg cagtatgtgg
       61 ggaacacagt tcggtgttct tgcctcacgg ctgtatctgg atccggagac cgcatcgatg
      121 cttcgggaga tgctggaaaa gcaggagaca ttttcaacat ttggcatgct gcatctgctg
      181 tgcatgacgc cggacatgtt ccggttttat ctgaaggcat ccgatgagcg gctggtggat
      241 caggtcttct gcgagcgggg tgatgaactg tggtgcgagg agttatccga ggagttcttt
      301 gcagcagtga aaacggcact ggttgcagag gcctggtgcg aggaggttcc cgaggagatc
      361 atctgcgaga acttcggtgt cggtgcagga gacattcatg cagtcgtgga aaatctccgc
      421 tggctgatgc acgcagcagg cagaattgct catgagtttg caccgcggtt tgatgccgat
      481 gtacgggatc ttgagatccg gatcgagaac ggtgtgaaag cagaactcct gccgctgatc
      541 tctctcaggg gcatcggccg catccgtgca cggcggctat tcaatcgtgg aatcaccaca
      601 cccgaagagc tgctcgcagc tgatacccgt gatgtggtgg caattcttgg aaacgttctc
      661 gcaaaaaatg tcttggaaca ggcggcgaaa aagacaggaa aaaccatgga aattgccgag
      721 ctcccggagg agaagaacga aacagttcct gctacagctt cagaagagga aaagccgcaa
      781 aaaacactat ttgattttgg agagtaaatg atgacggaac agttgttaac aatacatgag
      841 gcaaaagcca cggtggaaaa taccgcggcg gtgcttgcac agatcaataa gattgcagaa
      901 aaaaccggat caacgattgt actgtttgat gcggggaagg tggccggacc tgatcatatc
      961 cgctcggcgg tccggcatgc agaacgatcg tttgcgtccg gaaaaccggt tgcccggaca
     1021 ctcgcaatgg aaattcttct gtatgcatcc ggacagcggc agtgttctct tgcaccaaaa
     1081 ttcggtctgc atacaggaga gaatctgctg tttacggtaa ttcttggcgg tgatgcagcg
     1141 caggcacaac agctgctgaa cgggattgtt tcgttcacaa tgcacggcgt gactgcgtca
     1201 cgggcaactc tgatggagga gtttgggatc accgaggatg agattgcggt cgtcggagag
     1261 gatcgcatga atgagctggt aattgaacgg gttgcactga tggatgcata caaatgattc
     1321 agaggtactt ttccattaca taatcgtcca ttacaaatcc gtttccaatc tcggtcacca
     1381 gctcccgggc acgaacaaaa ccgagttttt cgtacaccgc aatggaggaa aggttctgct
     1441 tgttcactgt gagatagatc atgttcaatc cgtcgtcccg gcagagatcc gcaataaagt
     1501 tcatcgtcgt gcgggagtaa ccgctgccgc ggaacttttt tcggatgtaa atttttgaaa
     1561 gaaacattgc atgattcttt ggttcagggt gaacagccgt gtagccaatc cgatcagatc
     1621 ctaactgcag gagatagtac tgatagcagt cttcggtgat ctggcgatgt attgctttgg
     1681 cagactgaaa cgtttcgatc atgtacgcag tctgtgctgg accgatgatt ggagggaagt
     1741 gttccgtcca gatctcctgc gcaagttctg caacagctac aatttctgca tcagttttta
     1801 ccgaggaaaa ggagaggtcc ggcataatca ctgacctgca tgtgtaagga tgtgacgaat
     1861 ttcagggata atgatctctt ctgttgcaag ttttaccgct tttggcgatc ccgggatgca
     1921 gaaaattgcc tttccgtttg taattcctgc cgatgcacgg gaaaggataa ccgagtttcc
     1981 aacctgttca taactcttca tccggaacag ttcaccaact ccatccattg ttttttgaaa
     2041 cagcggagca actgcttcga tagtgcagtc atcatgcgta agtccggttc cgccattgac
     2101 tacaatacag tttgcaacat gcaatgcctc aaggactgcc gttctaatct cagagatatc
     2161 atccttaaca atgacaaccg aaacaaccgg aatgtctgcc gcagtaaatg catcgcggat
     2221 gatcttcccg ctcagatccg ttttttctgt acgtgtagtg gagacggtaa tcacaacagt
     2281 ctggatggaa atgtcctttt tgtgctcggc tggcatagca agtatgtagg aactcttcag
     2341 tattagcaat tcgtattcga ataagttcaa taaaaaacct tgaccattta caaaaaatgg
     2401 aaaacagatg tggtgtgata attttttgat gaggattttt gattctttgc gggaaactcc
     2461 cccacccctt tatttcgagt ggaacatatg gaagatatct tgccaagatc atctttccat
     2521 attgagttgt ac
//
LOCUS       DALTZZ010000063         1500 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_9891,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000063 DALTZZ010000000
VERSION     DALTZZ010000063.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9891"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /gene="pyrG"
                     /locus_tag="O0X12_01100"
     CDS             <1..1456
                     /gene="pyrG"
                     /locus_tag="O0X12_01100"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HJK52790.1"
                     /translation="IDAGLMNPAQHGEVFVLKDGGEADLDLGNYERFLDIELTRDHNL
                     TTGKIYSSVIQKERHGDYLGATVQIIPHITDEIKDRIKHAAESWTDKDGNHAEICIVE
                     VGGTVGDIESMPFLEAVRQMHWEYGNGDFVLVHVTLLPADTMGDLKTKPTQHSVKALR
                     ELGLEADLIVGRSTLPVSLSTKRKISSFCDVDVSAVISATTAPDTYLVPMELEKEGMA
                     DILLPLLKLSPGKPDNSWYSIVSREYTNRVRIGIITKYGVEDVYISIKEALKHAGRQL
                     STEVVIEWLDAEKYTTQDLADLDGILIPGGFGNRGIEGMIGAITYAREHKKPLLGLCL
                     GFQLSVIEFARNVVGYRDATSAEMGEGTPVIALLPEQEGVDDLGGTMRLGDYPVQLAA
                     DSRLAKLYGATEVVERHRHRYEVNPEYIEEIEAKGLRFVGKNGRRMEALELPDHPYFV
                     ATQFHPEFRSRPTRPSAPFIGFVEACLANHTKKE"
BASE COUNT          351 a          410 c          403 g          336 t
ORIGIN      
        1 cattgacgcg ggtctcatga acccggccca gcacggagaa gtctttgtgt taaaggacgg
       61 cggtgaggct gaccttgatc tgggaaacta tgagcggttc cttgacatcg agcttacccg
      121 ggatcacaat ctgaccaccg gaaagatcta ctcctctgtt attcagaaag agcgccacgg
      181 cgattatctt ggagcgaccg ttcagattat tccgcacatc actgatgaga tcaaagacag
      241 aatcaaacat gccgcagaaa gctggaccga caaggacggc aaccacgcgg agatctgtat
      301 tgtcgaggta ggaggaacgg tcggtgatat tgaaagtatg ccgttccttg aggcggtccg
      361 tcagatgcac tgggagtacg gcaacggcga ttttgttctt gttcacgtaa cccttcttcc
      421 ggcagacacg atgggtgacc tcaagacgaa accgacgcag cactccgtga aagcactgcg
      481 tgaactcggt ctcgaagctg atctgatcgt cggcagaagc accctgccgg tctcgctttc
      541 caccaagcgc aagatctcct ccttctgtga tgtggatgtc tccgctgtca tcagtgccac
      601 aaccgctcct gatacctacc ttgttcccat ggaactggaa aaggagggaa tggcagacat
      661 tctcctgccg cttcttaagc tttctcccgg aaaaccggac aacagctggt actccatcgt
      721 ctcccgcgag tacaccaacc gtgtcagaat cggcatcatc accaaatacg gtgttgagga
      781 tgtgtacatc tccattaaag aggcactcaa gcatgccggt cgtcagctct cgaccgaggt
      841 cgtcatcgag tggctggacg cggagaagta cacaacccag gaccttgccg atcttgacgg
      901 tattcttatc cctggaggat ttggcaaccg cggtattgag ggcatgatcg gtgcaatcac
      961 ttatgcccgt gaacacaaga aacccctgct tggtctgtgt cttgggttcc agctctccgt
     1021 tattgagttc gccagaaacg ttgtaggcta ccgtgatgca accagtgctg agatgggtga
     1081 gggaactcca gtcattgctc ttctgccgga gcaggagggt gttgatgacc ttggcggaac
     1141 aatgcggctt ggcgactatc cggtgcagct tgccgcagac tcgcggcttg caaaacttta
     1201 cggtgcaacc gaagttgttg aacgccaccg ccaccgctat gaggtcaacc ccgaatacat
     1261 cgaggagatc gaagcaaagg gtctgcggtt tgttggaaag aacgggcgcc gcatggaggc
     1321 tctggagctg ccggatcacc cgtactttgt tgcaacccag ttccacccag agttccggtc
     1381 ccgtcctacc cgcccctcgg caccgttcat cggttttgtc gaagcatgcc ttgcgaacca
     1441 caccaaaaaa gagtaaatca tggaatcgat tcttgtcctt gacttcggcg gtcagtacaa
//
LOCUS       DALTZZ010000064         2164 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_9923,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000064 DALTZZ010000000
VERSION     DALTZZ010000064.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9923"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            96..647
                     /locus_tag="O0X12_01105"
     CDS             96..647
                     /locus_tag="O0X12_01105"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HJK52791.1"
                     /translation="MVVPEKVFFTRGCGVHKDKLVSFEMALRDAGLAPYNLVTVSSIM
                     PPDAEIISREEGLPYLSPGEIVYCVMARTETNIPGQAVAASVGLAVPEIHGKQHGYLS
                     EYHAADISGHECGEYAEDLAATMLATIMDIPFDPETAWQEREQVYLASGKIIKTSHLA
                     ASAVCGDDGKWTTVIVAAVFILP"
     gene            711..2120
                     /gene="purF"
                     /locus_tag="O0X12_01110"
     CDS             711..2120
                     /gene="purF"
                     /locus_tag="O0X12_01110"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HJK52792.1"
                     /translation="MCGIAGIVANSEVSGSLYWALYALQHRGQESGGISTYDNGIVHK
                     YKGYGLVSEIFTSSILEKLSGKTGLGHVRYPTTGGSKPENIQPFNFLFRGHSLSIVHN
                     GNLVNTDELRYEYEGRGHIFSTTSDTEVIAAILANEIIHGHTVVEAISFCMRSIRGSY
                     AVIFMLDGVLYAFRDPLGIKPLCLGEFENGGHIVASESVALDAVGAKLIRDVAPGESI
                     RLMADKVYPHQIMTAKSPAHCVFEYIYFARADSVIDGISVYKVRQKTGFLLAGESPAH
                     ADIVSPVPDSGTAAATGYAAASGIPFREALIKNRYTGRTFIMPTQAKREMAVRMKLNP
                     VRGHITGKSVVLVDDSIVRGTTSRRIIALVREFGASQVHLRVASPPIIAPCYLGTDFP
                     TREELISHHKSVEGVREAIGATSLAHISLDGLVKAIGLPCSHLCTGCLTGKYPLAIDG
                     EDEDPRGIEMIDPYKNPEP"
BASE COUNT          492 a          551 c          574 g          547 t
ORIGIN      
        1 gtaattagcg agcacggtta ttaatttttg acatttggca taaggggggt aaatcacaac
       61 tattaatgag atgcacatca attttttaaa gaatcatggt tgttcccgaa aaggtatttt
      121 ttaccagagg atgcggagtc cacaaagaca agctggtgtc ctttgaaatg gccctcaggg
      181 atgcgggact tgccccgtac aacctcgtaa cggtctcctc gatcatgccg ccggacgctg
      241 agatcatctc ccgcgaggag ggactgcctt atctatcgcc gggtgaaatt gtctactgcg
      301 tcatggcgcg gacggaaaca aatattccgg ggcaggctgt tgccgcatcc gttggtcttg
      361 cggtaccgga gattcatggg aaacagcatg ggtatctctc cgagtatcat gccgcagata
      421 tctccggtca tgagtgcggt gagtatgctg aggatcttgc ggcaacaatg cttgccacca
      481 ttatggacat cccgttcgat cctgaaactg cgtggcagga acgcgagcag gtctatcttg
      541 caagcggaaa gatcatcaaa actagccatc ttgccgcatc cgcggtatgt ggtgatgacg
      601 gtaagtggac caccgttatt gttgccgctg tcttcattct tccataatat tctttttttc
      661 tgcgggatag gcgagtaact aattaccttc cagcgccaat acttctccac atgtgcggta
      721 tcgcgggcat cgtcgcaaac tccgaggttt cgggttcatt gtactgggcg ctgtatgcac
      781 ttcagcaccg cggacaggaa agcggtggaa tatccaccta tgacaacgga atagttcaca
      841 aatacaaagg atacgggctg gtgtccgaga tattcacgag cagcattctg gagaagcttt
      901 ccgggaaaac aggacttggc cacgtccggt atccgacaac cggagggtcc aaaccggaga
      961 atatccagcc gtttaacttt ttgttccgcg gtcattcgct ttccatcgtc cacaatggaa
     1021 atctggtcaa caccgatgag ctgcgttatg agtatgaggg caggggtcac atattttcca
     1081 ccacctccga tactgaagtg atcgccgcaa ttttggcaaa cgagatcatt cacgggcaca
     1141 ccgttgtcga agccatcagc ttctgcatgc ggagtatcag aggatcatat gcggtcatct
     1201 tcatgctgga cggcgttttg tatgcgttcc gcgatccgct cggcatcaaa cctctctgtc
     1261 ttggggagtt tgaaaacgga ggacatattg ttgcatccga aagtgttgct ttggatgcag
     1321 tcggtgccaa actgattcgc gacgttgctc ccggggaaag tatccgtctc atggcagaca
     1381 aggtgtatcc tcatcagatc atgaccgcaa aatctccggc gcactgtgtg tttgagtaca
     1441 tctattttgc ccgggccgat tcggtgatcg atggtatctc tgtctacaag gttcgtcaga
     1501 agactggatt ccttcttgca ggtgagtcac ctgcgcatgc tgacattgtt tcaccggtgc
     1561 cggactcagg aactgccgct gccacagggt atgctgcggc atcgggcatt cccttccgcg
     1621 aagcactgat caagaaccgc tacaccggtc gtacctttat catgccgacc caggcgaaac
     1681 gtgagatggc ggtccggatg aagctgaacc ctgtccgtgg ccacatcacc ggaaaatccg
     1741 ttgtgctggt ggacgacagc attgtcaggg gcacgacttc tcgccgcatc attgcgctgg
     1801 ttcgtgagtt tggagcaagt caggtacatc tccgggtggc atctccgccg atcattgcgc
     1861 cgtgttacct gggaaccgat ttcccgacgc gtgaggagtt gatctcacat cacaaatccg
     1921 ttgagggtgt ccgggaagcg atcggggcaa caagtcttgc ccacatctcc cttgacggtc
     1981 tggtcaaagc catcggactt ccgtgcagtc atctctgtac cggttgtctg acgggaaagt
     2041 atccgcttgc aattgatggc gaagatgagg atccccgcgg tattgagatg atcgatccct
     2101 acaaaaatcc tgaaccctga tttttttatt cagtacgtat ccataccgct agtctctgcc
     2161 atta
//
LOCUS       DALTZZ010000065         2332 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_9981,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000065 DALTZZ010000000
VERSION     DALTZZ010000065.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9981"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(12..155)
                     /locus_tag="O0X12_01115"
     CDS             complement(12..155)
                     /locus_tag="O0X12_01115"
                     /inference="COORDINATES: protein motif:HMM:NF003161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HJK52793.1"
                     /translation="MRFPEAEARLLNVKVCMKCNARNAIRATTCRKCGSTSLRPKNKE
                     RKA"
     gene            complement(218..760)
                     /gene="rdgB"
                     /locus_tag="O0X12_01120"
     CDS             complement(218..760)
                     /gene="rdgB"
                     /locus_tag="O0X12_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HJK52794.1"
                     /translation="MITVVTGNKNKAAEVAAFFAGITEVTHIAFDCIEPQAASVEDIA
                     RAKAEQAYAALGVPLIVDDTGLFIDALSGFPGPYAAYVQDTVRNTGVLRLMEGKEDRD
                     AHFTTAVAYADAAGIQVFSGRVDGVITTEERGNEGFGYDPIFAVGGKTLAEMTLAEKN
                     SLSHRARALAAFRDWYVGSA"
     gene            complement(757..2310)
                     /locus_tag="O0X12_01125"
     CDS             complement(757..2310)
                     /locus_tag="O0X12_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional N(6)-L-threonylcarbamoyladenine
                     synthase/serine/threonine protein kinase"
                     /protein_id="HJK52795.1"
                     /translation="MEGTAWNFSAAVFDEDLVCLHSSPYVPPHGGIHPREAAQHHAAV
                     AAEVIGKVLAEAGDEIDGVAFSIGPGLGPSLRTVATAARSLALKYDVPLIGVNHCVAH
                     VEIGRWYTKFSDPIVLYASGANTQVLGFLNGRYRIFGETLDIGLGNALDKFARSHDLP
                     HPGGPIIEEMAKKGSYIPLPYTVKGMDLAFSGLMSAAKDATARGESMEDVCYSFQETA
                     FAMCVEVTERALAHTGKDEVILVGGVGANSRLQQMLQTMCDERGAKFMAPPRVYMGDN
                     GAMIAYTGKVMLEAGSTIPIDQSFVNPSYRSDQVEVTWRSDAGELFAPGQSEITERGA
                     EATVDLTGTDAIKTRHSKGYRVPALDAHLITERTRAEARCIATARRSGVPVPVIRDLY
                     GSAIVMEKLTGDVLKYVISSDYAYAAGESVGKLHKAGIVHGDLTTSNMIWRNGRVYLL
                     DFGLAQMTDEIEPRGVDLHVLFQTLESTTSAPAELKQAFCEGYRSVFPEAEAVIKREQ
                     EIELRGRYL"
BASE COUNT          516 a          665 c          561 g          590 t
ORIGIN      
        1 cactgagccg cttatgcttt ccgttccttg ttcttcgggc ggagcgaagt ggagccgcac
       61 ttgcggcagg tggttgcgcg gatagcgtta cgtgcgttgc atttcatgca aactttcaca
      121 ttaagaagtc ttgcctctgc ttctgggaat ctcatgtatg aataacccct atattgagtt
      181 ctatattgtg ggtgctgaat gatattaaca attacgctca cgcggacccg acataccagt
      241 cgcggaatgc tgcaagggca cgtgcccggt gtgagaggga attcttttct gccaatgtca
      301 tctctgcaag tgtttttcca cccacggcaa agatgggatc gtagccaaac ccctcgtttc
      361 cgcgctcctc ggttgtgatg actccgtcaa ctcttccgga aaatacctgg attcccgcag
      421 catctgcata ggctactgca gttgtaaagt gtgcatcccg atcctctttt ccctccatca
      481 gccgcagtac gcctgtattc ctaacggtgt cctgcacata tgcagcataa ggtccgggaa
      541 atccggaaag agcatcgatg aagagtccgg tatcatccac gatcaacgga acaccgagtg
      601 ccgcatatgc ctgttctgct tttgccctgg caatgtcttc cacggaagct gcctggggct
      661 cgatacagtc aaaggcaatg tgtgtcactt ctgtgattcc ggcaaaaaat gcggcaacct
      721 cagcagcctt gtttttgttg ccggtaacaa ctgtgatcac agatatcttc cccgcagttc
      781 aatctcctgt tcccgtttga taaccgcctc agcttcgggg aacactgacc ggtacccttc
      841 acaaaatgcc tgtttcagtt cagctggcgc tgaggttgta ctctccagtg tctggaacaa
      901 aacatgcaga tccacaccgc gcggctcgat ctcatccgtc atctgtgcaa gtccgaagtc
      961 cagcagatac acgcggccgt ttctccatat catatttgag gtggtcagat ctccatggac
     1021 gattcctgct ttgtgcagtt taccaacgga ctctccggct gcataggcat aatcactact
     1081 gataacatac ttcagtacat ctcctgtcag cttttccatc acaattgcac ttccatagag
     1141 atcgcggatc accggaaccg gaaccccgct gcgtcgtgca gttgcaatac accgcgcctc
     1201 tgcgcgtgta cgttccgtaa tcagatgtgc atccagtgca gggacgcggt atcctttcga
     1261 gtgtcttgtc ttgatcgcat ctgttccggt aagatccact gtggcttctg caccgcgttc
     1321 cgtaatctcg gactggccgg gtgcaaacag ttctcccgcg tcactccgcc aggtaacctc
     1381 cacctgatcc gaccggtaac tgggatttac aaatgactga tcgattggaa tagttgaacc
     1441 cgcttccagc atcactttcc cggtataggc gatcatcgca ccgttgtccc ccatgtacac
     1501 gcggggtggt gccatgaact ttgcaccgcg ttcatcacac attgtctgaa gcatctgttg
     1561 cagacggctg tttgcaccta ctccgccaac cagaattacc tcatcctttc cggtatgggc
     1621 aagtgcccgt tctgttactt cgacacacat tgcaaatgcc gtttcctgga agctgtaaca
     1681 gacatcctcc atggactctc cgcgcgctgt tgcatccttt gcggcactca tgaggccgga
     1741 gaaggcaagg tccattccct ttaccgtgta gggaagcgga atgtaggatc ctttctttgc
     1801 catctcttca atgattgggc ctccagggtg cgggagatca tggctgcggg caaacttatc
     1861 cagtgcgttt ccaaggccga tgtccagcgt ctcaccgaaa atacggtaac ggccgttcag
     1921 gaagccgagt acctgcgtgt ttgctcctga cgcatacaac acaattggat cagagaattt
     1981 cgtgtaccaa cggccgatct ccacatgtgc gacacaatga ttcacaccga ttaatggtac
     2041 atcatacttc agggcaagag aacgcgccgc ggttgcaacc gttcggagtg acggaccaag
     2101 acctggtccg atggaaaatg ccacaccgtc gatctcatct ccggcttctg caagaacctt
     2161 tcctatcacc tcagcggcaa ctgctgcatg gtgctgggca gcttcgcgtg gatgaattcc
     2221 tccatgcgga ggcacatatg gtgaggagtg aagacaaacc agatcctcat caaaaacagc
     2281 ggcactgaag ttccaagctg tcccttcaat gccaagaacc cttgtttcgg gc
//
LOCUS       DALTZZ010000066         2407 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_10037,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000066 DALTZZ010000000
VERSION     DALTZZ010000066.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10037"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="O0X12_01130"
     CDS             <1..345
                     /locus_tag="O0X12_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HJK52796.1"
                     /translation="ASAEVRRSMKQIMWNNVGIYRNELDLRVAERDIAKLRAMPLGIS
                     SPREINDALITENMLLVASLVIEGALLRRESRGAHTRTDISIPWTNETSPFGHTFFSK
                     ARSGIEILEGRL"
     gene            342..1781
                     /locus_tag="O0X12_01135"
     CDS             342..1781
                     /locus_tag="O0X12_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HJK52797.1"
                     /translation="MKVQISRFDPDKDAAPHLETYDVHVENGARVLNVLDAVRDGIDP
                     TLGYRHCCRAGQCGSCAVRVNGEPVLACMEEAHDGDLIEPLSLPRIRDLITDIAPVMA
                     QMAWLNPGVEEISCSCGVTHDTIESIKPLRECIECYSCVSSCPAFAASEYAGPTIMRQ
                     EQRLNLDPRDGGDRVEEAIAKGIFACTTCHKCVEVCPKGIETPRKAVEKLRAEAAKRG
                     LSLPAHKALARLVEDTGHSVERKETPFLERVPDVIEPEGEVRATVGFFVGCMFNGRVI
                     QPALDAMEVLRKNGVRVVIPKDQVCCGSPLIRTGLSGFVPELMQRNIAAFVNAGVDVV
                     LTMCAGCGSTLKNDYNTPFRVADITEYLCEIGFVPPAKVAGTYTYHDPCHLLRGQGIS
                     EQPRELLRSVAETFVDLPARCCGAGGGVKSGRPEEAAAIGAVRAEMVKATGADFIVTV
                     CPFCEFHLHQVTGLPVKNIATLLLEGYRK"
     gene            1846..2109
                     /locus_tag="O0X12_01140"
     CDS             1846..2109
                     /locus_tag="O0X12_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HJK52798.1"
                     /translation="MDSSNLHRRLKKIIGQLNAIDKMIDEDVPCEDILIQINAAKSAM
                     HKVGQIVLEGHLNHCVKDGIEHGDADKTIAEFAKAIEHFSRMS"
BASE COUNT          565 a          567 c          650 g          625 t
ORIGIN      
        1 gcatctgctg aggtccgcag atcgatgaaa cagattatgt ggaacaatgt tggcatttat
       61 cgaaacgaac ttgatttgag agttgccgag cgtgacattg caaagctccg ggcaatgccc
      121 ttgggtatct cctcaccacg cgagattaac gatgcactca tcacggaaaa catgctgctt
      181 gttgcatcgc ttgtgatcga aggtgcactt ctccgccgcg agtcacgcgg cgcccatacg
      241 cggaccgata tcagcatccc gtggacaaat gagacctcac cgtttggtca cacattcttt
      301 tcgaaggcaa ggtctggtat tgagattctg gaggggcggc tgtgaaagta cagatctcgc
      361 ggtttgatcc tgacaaggat gctgcaccgc acctggagac gtatgacgta catgttgaaa
      421 acggtgcacg tgtactaaac gttcttgatg ccgtacgcga cggaattgat ccaacgcttg
      481 gatatcggca ctgttgtcgt gcaggtcagt gcggctcatg tgcagtccgt gttaacggag
      541 agccggtgct cgcatgcatg gaagaagcac atgacggaga tctgatagag ccgctttccc
      601 tcccccgcat cagggatctt atcaccgata ttgcaccggt gatggcacag atggcttggc
      661 tgaacccagg ggtagaggag atttcctgca gttgcggggt aacacacgat actatcgaat
      721 ctatcaaacc gcttcgtgag tgtattgagt gttacagctg tgtatcctct tgtcccgcat
      781 ttgcggcatc cgagtatgcc ggacctacca tcatgcggca ggaacagagg ttgaaccttg
      841 atccgcggga tggtggtgac cgtgttgagg aagcaattgc caaagggatc tttgcctgta
      901 cgacctgcca caaatgtgtg gaggtctgcc caaagggaat tgagactccg cgaaaggctg
      961 tggagaaact tcgagcggaa gctgcaaagc gtggcctgtc tcttcctgcc cacaaagcac
     1021 ttgccaggct tgttgaagat actggccact cggtcgaacg aaaggaaact ccgttccttg
     1081 aacgtgtgcc ggatgttatc gagcctgaag gcgaagtccg tgcaacggtc gggttcttcg
     1141 tcggctgtat gttcaatgga agagttattc agcctgcact tgatgcgatg gaagtgctcc
     1201 gcaaaaacgg tgtccgtgtg gtaattccaa aggatcaggt ctgttgtggt tcgccgctga
     1261 tccggaccgg tctttccggt tttgtaccgg agctgatgca gcgcaacatt gctgcattcg
     1321 taaatgcagg tgtggatgtt gtgcttacga tgtgtgccgg ctgtggttca accctgaaaa
     1381 acgattacaa taccccgttc cgggttgcgg atatcaccga gtatctctgt gagattggct
     1441 ttgttccccc cgcaaaggtt gcaggaacgt atacctatca cgatccctgt catctgcttc
     1501 gtggtcaggg gatctccgag cagccgcgtg aacttctgag atctgttgcc gagacatttg
     1561 ttgatctgcc agcacgctgt tgcggtgcgg gcggaggtgt gaagtcaggc aggcctgagg
     1621 aagctgctgc gatcggtgcg gttcgtgcag agatggtaaa ggcaactgga gcagatttta
     1681 ttgtaacggt ctgtccgttc tgtgagttcc acctgcacca ggtaaccgga ctgccggtga
     1741 aaaacattgc aacactgctg cttgaagggt atcggaaatg agcggcttta tcatctctca
     1801 cccgccaact atctatcagg gtaggtaatt ctcatgcgtc aatgtatgga ctcttccaat
     1861 cttcaccgcc ggttgaaaaa gatcatcggg cagctgaatg caatcgataa aatgatcgat
     1921 gaggatgttc catgcgagga tatcctcatc cagatcaatg cggcaaagtc cgcaatgcat
     1981 aaagttggtc agattgtgct tgaaggtcat ctgaatcact gcgtcaaaga cgggatcgaa
     2041 cacggtgacg cagacaaaac cattgctgag tttgcgaaag cgatcgagca tttttcgcgg
     2101 atgagttaac ccatctctat cctttttgtg aatttacatc tctattcaca tttttactct
     2161 ttttagatcg tacaacgcag ttgacatgat gatttttcaa aacactcata tacctatact
     2221 cccataggta ttatgtgagg gcggtgttat gaatgtttga aaaaatcata tctgtctttc
     2281 acgacgaaga aaagcgcagc gagatatgta tcatcctatc tgctgctgct gttctgatca
     2341 gtttcttctg gggacacaat ttcccagttg atcccggatg gattgctatc atcctctgtg
     2401 gtattcc
//
LOCUS       DALTZZ010000067         1795 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_10116,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000067 DALTZZ010000000
VERSION     DALTZZ010000067.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10116"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="O0X12_01145"
     CDS             <1..501
                     /locus_tag="O0X12_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HJK52799.1"
                     /translation="RLSADGRFGCDVPRLHLAQNLPFVPMVNAWKERRALLLERDMPD
                     ATGAIAQVSAPVLTNRAPPYSMPGGVFDAMMYCGGDMYAVSNADAAEAGRIFSDAENG
                     IDLDPAAAVAIAALISAVASGAVDSQDTITLNITGGGYARVREDLGIFPVPVTMTVDA
                     DEVPEI"
     gene            498..1607
                     /gene="comE"
                     /locus_tag="O0X12_01150"
     CDS             498..1607
                     /gene="comE"
                     /locus_tag="O0X12_01150"
                     /EC_number="4.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfopyruvate decarboxylase subunit beta"
                     /protein_id="HJK52800.1"
                     /translation="MNEEIVLRVLAEQGIDLVASLPCDKNKRLTALLPTKFPVIDLAR
                     EEDGVGICAGAYLGGRRPVMSIQSSGLGNMLNALMSLSRVYDLPLPILASWRGVYQEA
                     ICAQIPFNEPLPRVLEVYGIPYRIFSTGEDLIHLDEVIAGAYANRTPYVALIKPSCWE
                     PQEPVEIVYPPRESPARTLSLPGYGKPVMKRLDAVRVVAEYADADTIIVSNIGVPSKE
                     LYASKDRAGNFYMLGSYMQASAIGLGCAVALPHKRVIVIDGDGSLLGSAVLPVVAAEG
                     CSNLTIAALDNGTFGSTGNQMSPAYATADLGFLALGAGITSVERVCSADELREALDRR
                     TQFVHVLLSPGNSASPNIPLSPAEIRDRFVQFCKM"
BASE COUNT          358 a          407 c          546 g          484 t
ORIGIN      
        1 cggctttccg cagacggaag attcggctgt gatgttcccc gtctgcattt ggcacagaac
       61 ctcccgtttg ttccgatggt gaatgcatgg aaggaacggc gtgctctctt gctggaacgt
      121 gacatgccgg atgcaactgg agcgattgcc caggtgtccg caccggttct gacgaatcgt
      181 gcaccgccct acagtatgcc gggcggagtg tttgatgcga tgatgtactg cggcggtgat
      241 atgtatgctg tcagtaatgc ggacgcggcc gaagcaggac gcatcttctc tgatgcggag
      301 aacggcatcg atctggatcc ggcggctgcg gttgcgattg ctgctttgat ttcggcggtt
      361 gccagcggtg ctgttgattc gcaggatacg attacgctga atatcaccgg cggcgggtat
      421 gcacgggttc gtgaggatct gggtatcttc ccagttccgg tgacaatgac ggtggatgct
      481 gatgaggtac cagagatatg aacgaggaga ttgttctcag ggttcttgct gagcagggaa
      541 tcgatcttgt tgcatcactt ccgtgtgata aaaacaaacg gctgacggct cttctgccaa
      601 cgaagtttcc ggtgatcgat ctcgcacgcg aggaggacgg agtgggtatt tgtgcgggag
      661 catatctcgg cggcagacgg cccgtgatgt cgattcagag ctccggcctt gggaatatgc
      721 tgaacgcact gatgtcactt tcgcgggtgt atgatctgcc gctgccgatt cttgcaagct
      781 ggcggggtgt gtaccaggag gccatctgtg cacagattcc gttcaatgag ccgctgccaa
      841 gagttctgga ggtgtacggt attccctacc ggatcttttc aacaggggag gatctgattc
      901 atcttgacga ggtaattgcc ggtgcctacg cgaatcgtac tccctatgtt gcactgatta
      961 agccgtcgtg ctgggagccg caggagcctg tcgagattgt gtatccgccg cgtgaatctc
     1021 cggcacggac actgagtctt cccgggtatg gaaagccggt aatgaaacgg ctggatgcag
     1081 tccgggttgt tgcggagtat gcggatgcgg atacgattat tgtttccaac atcggtgttc
     1141 cgtcaaagga gctgtacgca tcaaaggatc gggcaggtaa cttctacatg ctgggtagtt
     1201 atatgcaggc gtcggcaatc ggacttgggt gtgcggttgc tctgccacac aaacgggtga
     1261 ttgtgatcga tggagacggg agtcttttag gttctgcagt actgccggtt gttgcggctg
     1321 aggggtgttc taatctgacg attgctgctc ttgataatgg tacgttcggg agtactggaa
     1381 atcagatgag cccggcatat gcaaccgcgg atctggggtt ccttgccctt ggtgcaggta
     1441 ttacttctgt ggaacgggtg tgttctgcag atgaactgcg agaggctctt gatcggagaa
     1501 cgcagtttgt ccatgtactt ctctctcccg gaaactctgc gtcgccgaac attccgttgt
     1561 caccggcgga gatccgtgat cggtttgtgc agttctgcaa aatgtgattc tgtcggatat
     1621 ttggaaaatt ctttttttct ttttgtttga aaattcttga aaaaagatcg cccacatttc
     1681 tttcaaaaaa gagattagta tctctcagga gttctcctga ggatttatca tctctttttt
     1741 tatttcacgc tgcttagttg cggcggagga cgagcagtgc tactgcacca agtcc
//
LOCUS       DALTZZ010000068         2270 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_10154,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000068 DALTZZ010000000
VERSION     DALTZZ010000068.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10154"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(134..1741)
                     /gene="ade"
                     /locus_tag="O0X12_01155"
     CDS             complement(134..1741)
                     /gene="ade"
                     /locus_tag="O0X12_01155"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HJK52801.1"
                     /translation="MIHVFDNAFLFNPCTGEWLLTSFSVENGIVAAIGANHSLTGDQI
                     TDLGGSRVIPGLIDTHLHIESTFLVPQEFGRVALAHGVTTAIADPHEIANVAGTAGID
                     FMLQDAQYAPNDIFFMLPSCVPATAEDVGGAVLTAADLAKYSDNPRVLGLGEMMNFPG
                     VLADDPEVLAKLSLFSHVDGHAPGLVGTDLCRYAAHGIKTDHECTSAEEAREKLRCGI
                     YILLREGEAARDVEALSSIVDASTVSRCCFCTDDRHVDTLVREGSIDHCIRIAIAAGM
                     PENLALRMATLSAAECFGLTDRGVIAPGRVADFCILADTEIFTIQKVYKNGIPADERP
                     VSANQTPYQVPPFSCRFPTQTDLSLPENGTARVIGIIPGELVTENIPARPDADGVQKL
                     LSIDRYHAGGFAIGLVKGFGMQTGAVASSIAHDAHNIIATGKTDEEILTAIRAVADAG
                     GGLAVVTGKEVTLLSLPIGGLMTYEPADSLLRGLDTLSAHLKRTGSCRQTFGHLSFLS
                     LTVIPHLKLTPRGLFDVDAFETVPLFH"
     gene            complement(1738..2148)
                     /locus_tag="O0X12_01160"
     CDS             complement(1738..2148)
                     /locus_tag="O0X12_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52802.1"
                     /translation="MMNKKAGQTVTIKRMVLVLIGIIAVLTLAIVLTVGATSIDADDP
                     VYINGKLVTAFEYNGEPRDVWVQCTISRVDDLFAEEQVGGLMTNAPTFTSGRNICEFP
                     IELEPGTYKVRIYIRERDEGAVRIAAFIKNFEIV"
BASE COUNT          602 a          601 c          545 g          522 t
ORIGIN      
        1 cagctgcagc tcctgccaat gcaggcagaa gaacaaatgc cgccgcaaga atgatggcaa
       61 tattccgctg tagcttcatc gtactaatca gtattgctgt tcttcctcaa tatcctttcc
      121 tgttgaagaa aaatcagtga aagagaggga cggtttcaaa cgcatccaca tcaaaaagac
      181 cccgcggcgt tagtttcaga tgcgggatta ccgtcagaga caggaaggag agatgaccaa
      241 aagtctgtct gcatgaacca gttcgcttga gatgtgcgga taatgtatcc agccctctca
      301 gcagggagtc ggcaggttca taggtcatga gacccccgat gggaagagaa aggagtgtga
      361 cctctttccc agtgacaacc gcaagacctc ctcccgcatc agcgacagca cggattgcag
      421 tcaaaatctc ttcatcggtt ttgccggttg caatgatatt gtgggcatcg tgggcaattg
      481 acgaggcaac agcccctgtc tgcatcccaa aacccttcac aagaccaata gcaaaacccc
      541 ctgcatgata gcggtcaata gagaggagtt tctgcactcc gtctgcatca ggtctggcag
      601 gaatattctc tgttaccagt tccccgggaa taataccgat gactcgggca gtaccgttct
      661 ccggcagaga aagatctgtt tgggttggga acctgcatga aaacggagga acctgatagg
      721 gtgtctggtt ggcagaaaca gggcgctcat cggcaggaat accgtttttg tagacctttt
      781 gtattgtaaa gatctctgta tctgcaagaa tacagaagtc agcaacccgc cccggtgcaa
      841 tcacaccccg gtcagtgaga ccaaaacact ccgcagcaga cagggttgcc atccggagtg
      901 cgagattctc cggcatgcct gcggcgattg caatacgaat acagtggtcg atggatcctt
      961 cgcggacaag cgtgtcaaca tgccgatcgt ctgtacagaa acagcagcgt gagacggtac
     1021 ttgcatcaac aatcgaagaa agggcttcaa catcccgtgc ggcctcaccc tcccgcaaca
     1081 gaatatatat accgcaccgg agtttttccc tggcctcctc tgccgaggtg cattcgtgat
     1141 ccgtcttgat cccgtgggcc gcataccggc agagatccgt tccaaccaga ccaggagcat
     1201 gtccgtccac atgggaaaac agagatagtt ttgcaagaac ctcagggtca tcggcgagaa
     1261 cccccgggaa gttcatcatt tccccaagac ccaagacccg aggattatca gaatattttg
     1321 caagatcagc cgcggtaaga acagctcctc caacatcctc ggcagttgca ggcacgcacg
     1381 agggaagcat aaagaaaata tcgtttgggg cgtactgtgc atcctgaagc atgaaatcga
     1441 ttccggcagt cccggccaca ttggcaattt catggggatc agcgattgcg gtggtaacac
     1501 cgtgcgcaag agcaacccta ccaaactcct gcggtaccag aaatgtactt tcaatatgca
     1561 ggtgggtatc gatcagtccg gggatgacac gtgatccccc aagatcggtg atctgatccc
     1621 cagtcagact gtgatttgca ccaattgcag caacaatacc attttccacg gaaaaagaag
     1681 ttagtagcca ttctccggta cacggattga agagaaaggc attgtcaaag acatggatca
     1741 tactatctca aaatttttaa tgaacgccgc aatccgtaca gctccttcat cccgctcccg
     1801 gatatagatc cgtactttat aggttcccgg ttccagttct atcgggaact cacagatatt
     1861 tctgccggag gtaaatgttg gtgcatttgt cataagaccg cctacctgtt cctcggcaaa
     1921 gagatcgtcc actcgggaga tcgtacactg cacccacaca tcacgcggct ctccgttgta
     1981 ctcgaatgca gtaacaagtt ttccgttgat atacacagga tcgtctgcat caatcgaggt
     2041 agcccctacc gtcaagacaa ttgccaaggt aagaactgca atgattccta tcaggaccag
     2101 taccatccgt tttatggtaa ccgtctgacc agctttttta ttcatcatta ttcatcagca
     2161 cgctctacga ttacacggat tttatcgccg tttttcaggc cgtgaccttt ggggacatca
     2221 atgttaaacc agagttcgcc ctgctgggac atcagacagt cttttgcccc
//
LOCUS       DALTZZ010000069         2945 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_10183,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000069 DALTZZ010000000
VERSION     DALTZZ010000069.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10183"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            120..740
                     /locus_tag="O0X12_01165"
                     /pseudo
     CDS             120..740
                     /locus_tag="O0X12_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448962.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
     gene            776..1120
                     /locus_tag="O0X12_01170"
     CDS             776..1120
                     /locus_tag="O0X12_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52803.1"
                     /translation="MTDNEKPVFFSTHLHDDLDHAFDDLRRIHDEITAVIRNMEDAAS
                     KCSDPEREKELRFYADAALGPTDDLEKWTTTYEDAVNEKLKGNHLVSCVCAGPSGLLS
                     LRNGSCRDSLHR"
     gene            complement(1160..2041)
                     /locus_tag="O0X12_01175"
     CDS             complement(1160..2041)
                     /locus_tag="O0X12_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HJK52804.1"
                     /translation="MKSDSKIYEMGGVITEADGEHCTVRLRMPAGIATADQLIAAAEL
                     AKKYGADELHFTTRQTLEIPHIPADQLPSFYEELIAAGNDVGAERGEVVNITACPGTH
                     RCKFANIDSVGMAKKIDAKYFGKDMPGKVRIAISACPNACTSETLNEIGITGICTPIR
                     QPGYCTGCGTCVQYCQEEALTVREGKIVMDDDACLDCGTCVRSCIYGILESQPQSYRI
                     TLGGRRGRHPKVGMHLITVNTEDAALQVIDSIIDWIYRYARFDTPIVQQIGQELDMED
                     LKKNLQKNIPAEQIVVL"
     gene            complement(2198..>2945)
                     /locus_tag="O0X12_01180"
     CDS             complement(2198..>2945)
                     /locus_tag="O0X12_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HJK52805.1"
                     /translation="LTTRQTIELLHVGPEILDDLLADLEKNGTPIGAERNEVVNVTAC
                     LGNANCKYSVIDTISLAKKLDEKHFGKEMPVKVRIAISGCPNGCTSERLNEIGITGLR
                     RPVRNEGLCTGCGTCGYYCREHAILIENGKLRLNQGDCMLCGFCIHACPFQYINFEDP
                     LYLITLGGRRGRHPKIGRTFYIARSEDDVVEIVGKIIYWIFRSIASDKMLPEQLTDEE
                     FAEFREKVVKSLKPLGIDGVDPTNAYCMQN"
BASE COUNT          714 a          655 c          751 g          825 t
ORIGIN      
        1 caagtgcaga gaagaatagg acagagatct gtatgtggag atgccacact ttctaactct
       61 gccccattct atgtggtagt aatagttcgg atgtttcctc acagtcagtt tgggctggta
      121 ctgcagctga gaccaagatc gtcttgaacg gcgactggac tcgtgtaaag cgtatcggcc
      181 agcagatgac tgctggtgag attgtgatca acagcaacgc cgatatgtac accggcggct
      241 ggatgaaggg cggcaagatt accgtaaacg gtaacgttga ctcctttact ggtattgcaa
      301 tggctggtgg agaacttacg gtaaacggca gtgcgcagaa ccacgtcggc tgtgcatacc
      361 gcggcgactg gcgcggtatg accggtggtg tcttccgcgt gaccggcaat gcaggtaacg
      421 atgtaggtac ctttatgcgc ggtggaacca tcatcatcga gggcgatgtg tttattcacg
      481 ttctgaccca cggcgagggt ggaaccatca tcatcaaagg aaatgtagag gggcgtgtcg
      541 gcggtcaggc agtaaagggt gacgtctatg ttttcggcaa gattgagtat atgatgcccg
      601 gatacagata cgttgacgat gttgaggccg aggttgacgg agaaaaggct gtctttgccc
      661 actacatcgg cgatcttggt gagcgccacc cgacatcaaa aggccagacg gtctatgtga
      721 acctctatct gaagaaataa ctttcttcag atcaaacatt ttttgagaga tttttatgac
      781 tgataatgaa aagccggttt tctttagtac ccatctccat gatgatcttg accatgcgtt
      841 tgatgatctg cggagaattc atgatgagat cactgctgtt atccgcaaca tggaggatgc
      901 ggcatccaaa tgttctgatc ctgaacggga aaaggagttg agattctatg cggatgcagc
      961 ccttggcccc actgacgatc tggagaagtg gaccacgacc tatgaggatg cagtgaatga
     1021 gaagctgaaa ggaaatcatc tggtatcctg tgtttgtgct ggaccgtcag ggttactgtc
     1081 tttgcggaat ggatcttgcc gagattcgct acatcgatga agtccgcgat ctgatggcag
     1141 aaaaataatt tttttgtttt tacagcacta caatctgttc tgcggggatg ttcttctgca
     1201 gattcttttt gagatcttcc atatcgagtt cctgtccgat ctgttgaacg atgggggtat
     1261 caaatcttgc gtaccgatag atccagtcaa tgatactatc gatcacctgg agtgcggcat
     1321 cttcagtgtt gacggtgata agatgcatgc ctactttggg gtgacgtccg cgtctgccac
     1381 caagggtaat gcggtaggac tggggttgtg actcaagaat tccgtagata caggagcgga
     1441 cgcaggttcc gcagtcaaga catgcgtcat cgtccatgac gatttttcct tcgcgaactg
     1501 ttagtgcctc ttcctggcag tactgtacac atgtcccaca ccctgtacag tagcctggct
     1561 ggcggattgg tgtacagata ccggtgatgc ctatttcatt cagggtttca ctagtacagg
     1621 cattcgggca tgctgatatg gcaatacgga cttttccggg catgtccttg ccgaagtatt
     1681 tggcatcaat tttctttgcc atgccgaccg aatcgatgtt ggcaaattta cagcggtggg
     1741 ttccggggca ggcagtgatg tttacaactt ccccgcgttc ggcaccaaca tcatttcctg
     1801 cggcaatgag ctcctcgtaa aatgatggga gctggtccgc agggatatgc gggatctcga
     1861 gtgtctgccg ggtggtgaag tgaagttcgt ctgctccgta ttttttggcg agctccgctg
     1921 cggcaataag ctgatcagct gttgcgatcc ccgcaggcat tcgtaaccgg acagtacagt
     1981 gttccccgtc tgcttcggta attactcctc ccatttcgta tattttggag tcggatttca
     2041 tctatttata tttgtatgct gtatttatta aggtgagttc tgagtgcttt tcccctagta
     2101 tatatttatc cataatatga tggagaccgg tcctatattt ttgatacatg taacgtgtca
     2161 gggataattg tctgaaaaaa aaagatggtg aaagatatta attttgcata cagtaagcat
     2221 tagttggatc cacaccgtca atgccaagcg gtttcaaact cttcacaact ttttcgcgga
     2281 actcagcaaa ctcctcatca gtaagttgtt ctgggagcat tttatctgat gcgatacttc
     2341 tgaaaatcca gtagatgatc tttcccacga tttccacaac atcgtcctcg gagcgtgcga
     2401 tatagaatgt tcttccgatc tttggatgac gtccgcgtct accaccaagt gtaattagat
     2461 agagaggatc ctcgaaatta atatactgga aaggacatgc gtgaatgcag aatccacaga
     2521 gcatacaatc tccctgattt aaccgaagtt ttccgttttc aatcaggatt gcatgttccc
     2581 ggcagtagta tccgcaggtt ccgcatccag tacaaagccc ctcattacgt actggccgac
     2641 ggagtccggt aatgccaatc tcatttaatc gttcactcgt acacccgttc gggcagccgg
     2701 agatcgcaat gcgaactttc acaggcatct cctttccgaa atgtttctca tcaagttttt
     2761 tggcaagtga gatggtgtcg ataacggagt acttacagtt tgcatttcca aggcatgcag
     2821 tgacatttac gacttcatta cgttctgcac cgatcggagt cccgtttttc tcaagatccg
     2881 caagaagatc atcgagaatt tcagggccta cgtgaagcaa ctcaattgtt tgtcttgttg
     2941 ttaag
//
LOCUS       DALTZZ010000070         2308 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_10529,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000070 DALTZZ010000000
VERSION     DALTZZ010000070.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10529"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /gene="mmp10"
                     /locus_tag="O0X12_01185"
     CDS             complement(<1..1095)
                     /gene="mmp10"
                     /locus_tag="O0X12_01185"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844472.1"
                     /note="Mmp10 (methanogenesis marker protein 10) is a
                     cobalamin-requiring radical SAM methyltransferase that
                     creates the methylarginine modification to methyl coenzyme
                     M reductase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase-arginine
                     methyltransferase Mmp10"
                     /protein_id="HJK52806.1"
                     /translation="MVHLTVDIGGRPGVDCRGFCAYCYFKHVKDVPPFGCRYCLPFLK
                     GCDYCSRGVKEEYSGFLPLRTVAESFLADLQMVTEDVTRITISGGGDPRCYPDFRDLV
                     ELLGTLDIPLHIGYTSGKGFDEPEIADFLVENHLTEVSFTVFAADAELRKKWMHDPTP
                     EASLEIIRRLAKKIDVYAAVVVLPDVNDGDVLTKTLSWLSDIGVKGVILMRFANRPEQ
                     GLILENAPILPGQRVHTVEEFADLVQAGAAAFPYLRISGTPLYDPLFDSPFAIRKMPE
                     LLDHLPKINKKATIITGSVAATYIKEIIRSRGGDPDSVIAVNKEIACLITIEDLTSLN
                     LQDLSDTVIIPGRAFVHDAEAEKVLSRDGVY"
     gene            complement(1339..1608)
                     /locus_tag="O0X12_01190"
     CDS             complement(1339..1608)
                     /locus_tag="O0X12_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5350 domain-containing protein"
                     /protein_id="HJK52807.1"
                     /translation="MGKTGSINWHQIKGVNGQIRLVPQKEGEVKKPGPNQRFKRASIV
                     TKIESRMANAPQQGRRGPKIADPRVRRRIRRSKKSMMGAKAKSKR"
     gene            complement(1707..>2308)
                     /locus_tag="O0X12_01195"
     CDS             complement(1707..>2308)
                     /locus_tag="O0X12_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HJK52808.1"
                     /translation="AARMYPETDILPVPVTQEYWESITTPELLIDKAERFVHEYGLDA
                     GMAHQIAFSEKLPLFERAVAEGIRPVFAARTILASLKELSRAGLSPDAIPDDSVIAVM
                     KAVETGHAAKEAVSEILSACARGMTLEEAIETVAPAFSREELQTLVHTIVSERVDFIK
                     SRGKAALGPVMGVVMKEVRGRIDGKVVSEVLEEELGRII"
BASE COUNT          563 a          591 c          535 g          619 t
ORIGIN      
        1 atacacccca tcacgcgaca gtactttttc tgcctctgca tcatggacga atgcacggcc
       61 gggaataatc acggtatcag acagatcctg caggttcagt gaagtaagat cctcaatggt
      121 tatcagacat gcaatctctt tgttcactgc aatgaccgag tctgggtctc cgccgcggga
      181 tctgattatt tccttaatat atgtggctgc aacagatccg gttataattg tagctttttt
      241 attaattttt gggagatgat ccagcaactc tggcattttt cggatggcga atggagaatc
      301 aaacagaggg tcatacaatg gagtcccgga tatcctgaga tagggaaatg cagcagcacc
      361 cgcctgtaca agatctgcaa attcctcaac tgtgtgtacg cgctgacctg ggagaattgg
      421 ggcattctca agaatcagtc cctgttcggg cctgtttgca aaccgcatca gaatgactcc
      481 ctttaccccg atatcagaga gccatgagag tgttttggtc aacacatcac cgtcgtttac
      541 atccggcagg acaacaaccg ctgcatacac gtcgatcttc tttgcaagac gacggatgat
      601 ctcaagtgat gcctcaggcg ttggatcgtg catccatttt tttctgagtt ctgcgtcagc
      661 ggcaaatacg gtgaaggaga cctctgtaag gtgattttca accaaaaagt ctgctatctc
      721 tggctcatca aatcccttgc cgctggtgta gccgatgtgc agcggtatat ccagtgttcc
      781 aagaagctct accagatcac gaaagtcggg ataacatctg gggtcgccgc cacctgagat
      841 ggtgatgcgg gtgacatcct ctgtgaccat ttgaaggtct gcaagaaatg attcagcaac
      901 ggtcctcaaa ggcagaaatc ctgagtactc ctctttgaca ccgcgggagc agtagtcaca
      961 tcctttcaaa aacggaagac agtacctgca gccgaacgga ggtacatcct ttacatgctt
     1021 aaagtagcag taggcacaaa aaccccggca gtcaacaccg ggacgtccgc ctatatcaac
     1081 ggtaagatgc accatagtct cttctatatc cattagtgct acggggatat gaatggttga
     1141 gataaatggc gttttctcgt ctagcaaatc ctactttttc atgacatcat gctagagagt
     1201 ttttcattta ggatctttgt agacagagtc tactatcacg aaataaacag atgagtttct
     1261 tcagcaaaca gaaaaaattg gaaatgtact cccgtgggaa tacataaatt ggggatggaa
     1321 gtattgtgtc cgaaaagatc agcgctttga ctttgctttt gcgcccatca tggacttctt
     1381 ggatctccgg atccggcggc gaacgcgcgg gtctgcgatc tttggtccac ggcggccctg
     1441 ctgtggggca tttgccatac ggctttcgat cttcgtaaca atgcttgctc tcttgaatct
     1501 ctggttcggt cctggtttct tgacctctcc ctctttctgg ggaacgagtc tgatctgacc
     1561 gttaacacct ttgatctggt gccagttgat gctacctgtc tttcccatat tgaactcctt
     1621 accttgttcg tgccctattc acttaaacat atctcaatga ctgcggagta gtgtaatctc
     1681 cttgaaagca gatctgatgg agcggatcag atgatccgtc caagttcctc ctccaaaacc
     1741 tccgagacca ccttaccatc aatcctgcca cgcacctctt tcatcactac tcccatcaca
     1801 ggtccgagtg ctgcctttcc gcgtgatttt atgaaatcaa cacgttcgga aacaattgta
     1861 tgaacgaggg tctgcagttc ctcgcgggaa aatgcgggtg caaccgtctc aattgcttct
     1921 tccagggtca tgccgcgtgc acatgccgat aatatctctg acactgcctc ttttgctgca
     1981 tgtcccgtct caactgcctt cattactgcg atgacagagt catccgggat tgcgtctggt
     2041 gagagtcccg cacgcgacag ttcttttagg gaagcaagta tggtgcgcgc cgcaaacaca
     2101 ggacgaattc cctcggcaac tgcccgttca aacagtggca gtttctcgga aaatgctatc
     2161 tgatgtgcca tccccgcatc aagaccgtac tcatggacaa accgctcagc tttgtcgatc
     2221 aaaagttccg gcgttgtgat actttcccag tactcctgag tgaccggtac tggtagaata
     2281 tccgtttccg gatacatacg cgctgcac
//
LOCUS       DALTZZ010000071         2629 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_10639,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000071 DALTZZ010000000
VERSION     DALTZZ010000071.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10639"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            117..683
                     /gene="pdxT"
                     /locus_tag="O0X12_01200"
     CDS             117..683
                     /gene="pdxT"
                     /locus_tag="O0X12_01200"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HJK52809.1"
                     /translation="MRIGVLALQGAFAEHIARLSSLGADTFEIRKAADLQEPCDGLVI
                     PGGESTTMTKLLHDLGMFELLRERIVSGLPVMGTCAGLILLAKRVEGGIPCLATMDIT
                     AVRNAYGRQLGSFQTNAVVAGVGEVPMTFIRAPCVRDPGSAVTVLAEVDGRIVAVREG
                     NQLALAFHPELDPDTRIHEYFLAMAAGN"
     gene            complement(755..1435)
                     /locus_tag="O0X12_01205"
     CDS             complement(755..1435)
                     /locus_tag="O0X12_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52810.1"
                     /translation="MKKIMMILVAAVVLALFVGAGSADTAVGGDRGIIQVHSNVDGAS
                     VVLVSINGDESSPQTITNGQTEFAVYTTATPYKEVKVSADGYETATAAVTLPAKGETS
                     VVEVELVPAQMVGGDRGVIQVRCDVNGATVELISVSNTVAYTGTIENGQAEFSVYTTA
                     TPITQARVSANGYETATVPVTMPAKGETSVVDVTLTPVPTPTQSPMGLALVGLLGAIG
                     AVALLRRE"
     gene            1571..1987
                     /locus_tag="O0X12_01210"
     CDS             1571..1987
                     /locus_tag="O0X12_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HJK52811.1"
                     /translation="MHPYIEKIATIGSAANPTFQTLGIEPVSWGDGSAVLKMTVTPNL
                     HNGVGFLQGGFYVILADEAIALAVLAVAGDDEGTATISETTHFIRGVCEGEIYGVARI
                     TRKGRRIVFAEGEVRKGSPNGELLSKTVVSYTMTRG"
     gene            complement(1998..2480)
                     /locus_tag="O0X12_01215"
     CDS             complement(1998..2480)
                     /locus_tag="O0X12_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine adenylyltransferase"
                     /protein_id="HJK52812.1"
                     /translation="MKVMVGGTFDPLHIGHQLLLRRAFMTAGPGGHVVIGLSADPFAA
                     RKQHPVRCYDVRFAELTAWIDGQNFGATYDIEPLYDQYGSALTQDFDALVVSYETFPV
                     GNEINRKRKEMGKAMVDLYQIQCVLAEDGKAVSSTRIYRGEINRYGEPVAEEEFFSGE
                     "
BASE COUNT          570 a          653 c          778 g          628 t
ORIGIN      
        1 actataatga tccaaagatg cttgcagagc tttccgagga tctcggcgag gcgatggtcg
       61 gtatcaacgc ggacgagatc gccattctga tggcggaacg gggccagtaa acgtctatgc
      121 ggattggtgt tcttgccctg cagggtgcat ttgcggaaca tattgcacgg ctttcctccc
      181 ttggtgcgga tacgtttgag atccggaagg ccgcagatct tcaggagccg tgtgacggtc
      241 tggtgattcc aggcggcgag agcacgacga tgacaaaact cctgcatgat ctcgggatgt
      301 ttgagctgtt gcgggagcgg atcgtaagcg gtcttccggt tatgggtacc tgtgcgggac
      361 tgatccttct tgcaaaacgc gtcgagggcg gcatcccctg tcttgcgacg atggatatta
      421 ccgcggtccg gaacgcctac ggaagacagc tcggcagttt ccagacaaat gccgtggttg
      481 cgggtgtcgg cgaggtgccg atgaccttta tccgtgctcc ctgtgtccgc gatccgggat
      541 cagcagttac ggtgctggct gaggtggacg gacggattgt tgcggtgcgg gaaggaaatc
      601 agctggcact tgcattccac cccgaactgg atccggatac acggattcac gaatacttcc
      661 tggcaatggc tgccggaaac tgattcattt ttttggtttt ggatcggaac tcctgcggtt
      721 cgcaggaaaa aaagaggaaa tgattttttg ttgattactc tctgcggagc agtgcaactg
      781 caccgatggc tccaagaaga ccaacgaggg caagtcccat cggggactgg gtcggtgtcg
      841 gaaccggggt cagggtgaca tcaacaacag aggtctcgcc ctttgcaggc atggtgaccg
      901 gtactgttgc agtctcgtaa ccgtttgcgg agactctggc ctgggtgatc ggggttgcgg
      961 tggtgtagac cgagaactca gcctggccgt tctcaatagt accggtgtag gcaaccgtgt
     1021 tggagacgga gatgagttca acggttgcgc cgttaacatc gcagcgtacc tgaatgacac
     1081 cgcggtcgcc gccgaccatc tgtgcgggga caagttcaac ctcaacaaca gaggtttcgc
     1141 cctttgcagg cagcgtgacc gctgcggttg cggtttcgta tccgtctgcg gagaccttaa
     1201 cctccttgta cggggttgca gtggtgtaca ccgcaaactc ggtctgaccg ttggtgatcg
     1261 tctgcgggct gctctcatcg ccgttaatgg aaactaagac tacgcttgca ccgtcgacgt
     1321 tgctgtgcac ctgaatgata ccgcggtcgc cgccgacagc agtatctgcc gagcctgcac
     1381 caacaaagag tgccagaaca actgcggcaa caagaatcat catgatcttt ttcattatat
     1441 ttttctcctc caccctccat gccgggtggt tggttaagta aactattttc tgccaatagc
     1501 atgtaaagtt tgttcttatg caggaaaaac cggaaatcct tttttcgtgt cctgcaaata
     1561 cgattctgca atgcacccat acatagaaaa aattgcaaca atcggatctg cggcaaatcc
     1621 gacgtttcag acgcttggca ttgaaccggt ttcctggggc gacggatctg cggttctgaa
     1681 gatgaccgta acaccgaacc tgcataacgg tgtcgggttt ttgcagggcg gattttatgt
     1741 gattctggcg gatgaagcga ttgcccttgc ggttcttgcg gttgcggggg atgatgaggg
     1801 cactgcaacg atctcggaga caacgcattt catccgcggt gtctgtgagg gagagatcta
     1861 cggtgttgcc cgcatcacgc ggaaaggcag acggattgtg tttgcagagg gagaggtgcg
     1921 gaaaggttcg ccaaacggag aacttttgtc aaaaacggtt gtgtcctata cgatgacacg
     1981 gggataagag gagaacgtta ctctcctgaa aagaactcct cttctgcaac cggttcgccg
     2041 taccggttga tctcaccgcg gtaaatccgg gtggaagaga cggctttgcc gtcctcggca
     2101 agtacacact ggatctgata gagatccacc atcgcttttc ccatctcctt tcgtttgcgg
     2161 ttgatctcgt ttccgaccgg aaatgtttcg tagctgacga caagtgcgtc gaagtcctgg
     2221 gttagtgcac tgccgtactg atcgtacagg ggttcgatgt cataggttgc cccaaagttc
     2281 tggccgtcaa tccatgcggt cagttccgcg aaccggacgt catagcatct gaccggatgc
     2341 tgttttcgtg cggcgaacgg atccgcggac agaccgatca cgacatggcc gccgggtccg
     2401 gctgtcatga atgcacgccg cagcaggagc tggtgcccga tatggagggg atcgaatgtc
     2461 ccgccgacca tgactttcat tgcggttcta ttgatcggat acagggaaaa aaggtttggt
     2521 atgcaggaag ttctgacaag accgggtggt ggaaggccgg ggctgatcct ccccattcga
     2581 aatcctgata actccccgca ttgaactata gtaccattat ggataacag
//
LOCUS       DALTZZ010000072         4101 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_10756,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000072 DALTZZ010000000
VERSION     DALTZZ010000072.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10756"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="O0X12_01220"
     CDS             <1..1296
                     /locus_tag="O0X12_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52813.1"
                     /translation="LTMKKTKNLGYPGKTAKLLFLAVLLACTVIVPVSAEDTPALFTP
                     LEIQEIMTAGLVFSDVFNDSLYNSLDDSAKADYMATVLVCLSEPANRTAVSMNNVPGL
                     LSTTMNQQAQILYQSRTNHILQQYIPSMYSTPLINRESIYYNVDTKTFTFRYSSGVLA
                     MVKLVKDECCNEANESISFTKIQTSTLPDELLSDDTQIRAVSGDKALLIYDIDTTGSR
                     LYNLEYVRDFLLENNPPVTSIIKQNPTVPEYRTLLSATVVSSGYRAVIISSHGDVYSL
                     NLFTNYPSVTLCETTNDVKLDLYSEDLLQNRVGIVHQPEEADRFSLLPSFFEYYYSGN
                     KLNGAYVHLGICKGFGDGLFVDNTLASKFTASGASVVTGYHKEVFIPYDHGMIKSISR
                     NLANGSSLESSVNSAKAVHGDTGPNGEYIIVYGNGGWML"
     gene            1374..1958
                     /locus_tag="O0X12_01225"
     CDS             1374..1958
                     /locus_tag="O0X12_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52814.1"
                     /translation="MSRCETLKLIAVYAVLIAVICLAIFAVIQDINEQRTTPDISLSR
                     LGIPEKDGLSIVSHGVEITTEQEEEVISMVEEFVQENYDGWYAMGYIQVREAATHGVA
                     IEISYNPAWELTYQSFDETHRPNATVLIDRIYVTIPDLPDKRNKLYYYVSPVCDGDLD
                     HTILLPIEKANELLELIGEDPIPPWKTSLIHLLS"
     gene            complement(2062..3537)
                     /locus_tag="O0X12_01230"
     CDS             complement(2062..3537)
                     /locus_tag="O0X12_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52815.1"
                     /translation="MSDSDNLGAFFGTVRGAGAGTRLFSWKKICDAASVAEAEYTDLV
                     EHIAQTEKRLADTESLATSREAIIDGLNTRISEMTSKYEARNADADRLSKEKSLLEGK
                     LSGLTVTAAEHERSAEKWKAAESSCRTELAALQSQFEELSRERDALAGDLATERSAHL
                     ADNEQARTFREQYLSREAEIDALRRELDAAGKEIAARQAEIDSYIEERDTKYTVLAAE
                     AERLSALSSKLAEERKAEEEEAEQRMQEIFAEHHDRVGEILERVCSELYLDLRTAETY
                     RGEGSPEFAVLVNSSYVFFNTAAPASSAEAEEFAARISGEAKLLFEAAKEDGVRGEAY
                     LVVPNTVAAQLTELVFSSERCTVFVVTPEAVEPIVRTLQRIEEYEFARQITPFELDQI
                     CRFVGELSHMTKRKIVLDTYFSNEMLEILQKTENLPTDVVADVTAYENAVRLNPPAER
                     DATVLTVPSLSAKVQKLEKAMLARAAAEAEEEVADTQPAKE"
     gene            complement(3628..>4101)
                     /locus_tag="O0X12_01235"
     CDS             complement(3628..>4101)
                     /locus_tag="O0X12_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52816.1"
                     /translation="DTKSSLEADLSAKQQTLASYSAITREILEEWKNHRDETCSCIAD
                     ATGTVPACEHHVFIADISGHRVMFLAFAPASYEGLREADTFVRDAARKLAETVGPSGC
                     RKEAFVVVPNECMLYLTKCLYASSLCSVQVITPSQVASAVRTVARFLVYEKTQNP"
BASE COUNT         1111 a         1079 c          876 g         1035 t
ORIGIN      
        1 ctgactatga agaaaacaaa aaatttaggg tatccaggta aaaccgccaa gctactgttc
       61 cttgcagtac ttcttgcatg tacagtaatt gtcccagtat cagccgagga tacccctgct
      121 ttgtttacac cactggaaat ccaggaaatt atgaccgcag gattggtttt ctccgatgtg
      181 tttaatgatt ccctctacaa ttctcttgat gacagtgcca aagcagatta tatggcaaca
      241 gtacttgtgt gtttgtccga accagcgaac agaactgctg tgtcaatgaa caatgttcct
      301 ggattactgt ccacaactat gaaccagcaa gcacagattc tgtatcaatc acggactaac
      361 catattttgc agcaatatat tccttcgatg tactctacac cgctcattaa tcgtgagtcg
      421 atctattata atgtagatac gaagacgttt acgtttagat acagcagtgg agttcttgca
      481 atggtaaaac tggttaagga tgagtgttgt aatgaagcaa acgaatcgat ctcatttaca
      541 aaaatacaga catcgacatt gcccgatgaa ctgctttcag atgatacaca gatacgggca
      601 gtatctggtg ataaagcatt attgatttat gatatagata caacaggtag tagattatac
      661 aacctcgagt atgttaggga tttcttatta gaaaataatc ctccagttac tagtatcatc
      721 aaacaaaacc caactgttcc tgaatataga acattgttat cagcaactgt ggtgtctagt
      781 ggttatcgcg cagtcattat ttcatctcat ggagatgttt attctctgaa tctctttact
      841 aattatccaa gtgttactct ttgtgaaact accaacgatg tgaaacttga tctatactca
      901 gaagatttgc ttcagaatcg tgtcggaata gttcaccaac cagaggaagc tgatagattt
      961 tcccttttgc caagtttctt tgagtattat tacagtggta ataaactgaa tggagcatat
     1021 gtacacttag gaatatgcaa aggattcggt gatggtttat ttgtcgacaa cacccttgca
     1081 tctaaattta ctgcgtctgg tgcaagtgtg gttacaggat atcataagga agtatttatt
     1141 ccatatgatc atggtatgat taaaagtatt tcgagaaatc ttgccaatgg tagctcatta
     1201 gaatcatctg taaatagtgc aaaggcagtt catggagata caggaccaaa cggagaatac
     1261 atcatagtat acggtaatgg aggatggatg ctttaatcat aacccatata tatattttaa
     1321 tttgttctat tgtggtaatg gaaatacaga tagctgagag taaatgatcc aaaatgtccc
     1381 gttgtgaaac cctcaaactt attgcagttt acgcagtcct gatcgcagtc atctgtctcg
     1441 ctatatttgc tgtcattcag gacatcaacg aacaaaggac tactcctgat atttccttat
     1501 cacgtctggg aattccggag aaggacggtc tttccatcgt ttcccacggg gtggaaatca
     1561 ctacggaaca agaagaggaa gtcatcagca tggtagagga gtttgtgcag gaaaattatg
     1621 atggttggta tgccatggga tatattcaag tacgggaggc agccacgcat ggggttgcaa
     1681 tcgagatcag ctataacccc gcatgggaac ttacctacca gtcttttgat gaaacacatc
     1741 ggccaaacgc cactgtactt attgaccgga tctatgtaac cattcctgat ttaccggata
     1801 agcggaataa actgtactat tatgtttccc ctgtttgtga tggagatctg gatcatacta
     1861 ttctcctgcc aattgagaag gcgaatgaac ttctggaact catcggtgaa gacccaatac
     1921 caccatggaa aacgtccctg atacaccttc tatcctgaga aaacacctct ctatttcctt
     1981 cgtccccaaa ttctggattc ggataaaact ttccggcaga aaaactctgc aaaaaagggt
     2041 gattatgaga gattacaaaa attattcttt tgcaggttgc gtatcggcaa cctcctcctc
     2101 agcctcggca gcagcacggg caagcatcgc cttttccagc ttctgcacct ttgccgaaag
     2161 cgacggaaca gtcagaaccg ttgcatcccg ctccgccgga ggattcagcc gcaccgcatt
     2221 ctcatacgcc gtaacatcag ccacaacatc ggtcggcaga ttctcggtct tctgtaaaat
     2281 ctccagcatc tcattactga agtacgtatc cagcacaatc ttcctcttcg tcatgtgcga
     2341 cagctcgccg acaaaccggc agatctgatc cagctcaaac ggtgtaatct ggcgtgcgaa
     2401 ctcatactcc tcaattctct gaagtgtccg aacgatcggc tcaaccgcct cgggcgtcac
     2461 cacaaacacc gtacaccgct ccgacgaaaa caccagctcc gtaagctgtg cggcaaccgt
     2521 gtttggcacc acaagatacg cctcaccgcg gacaccgtcc tcctttgctg cctcaaacag
     2581 cagcttcgcc tctccagaaa tccgggcagc aaactcttcg gcctccgcag acgatgcagg
     2641 tgccgccgta ttaaagaaca cataactgct gttgaccaga accgcaaact caggagatcc
     2701 ctcaccgcgg taggtctccg ccgtcctcag atccagatac agctcggaac agacccgctc
     2761 cagaatctca ccgacgcgat catgatgttc tgcaaagatc tcctgcatcc gctgctcggc
     2821 ttcctcctcc tccgccttcc gctcctcggc aagctttgac gaaagagcac tcagacgctc
     2881 ggcctctgcc gcaagcacgg tgtacttcgt atcgcgttcc tcgatatagg aatcgatctc
     2941 cgcctgccgt gcggcaatct cctttccggc agcatcaagc tcccggcgca gtgcatcaat
     3001 ctccgcctca cgcgacagat actgctcacg aaacgtccgt gcctgctcat tatccgcaag
     3061 atgcgccgac cgctccgtgg caagatcccc ggcaagagca tcacgttcgc gggacagctc
     3121 ctcaaactga gactgaagag ctgcaagctc cgtccggcag gaactctctg cggctttcca
     3181 cttctcggca gaacgctcat gctcggcagc ggtcaccgtc aggcctgaaa gctttccctc
     3241 aagaagactc ttctcctttg aaagccgatc cgcatccgca ttgcgtgcct cgtacttgga
     3301 cgtcatctca cttatccggg tgttcagccc atcaataata gcctcccggg acgtggcaag
     3361 actctcggta tctgcgagcc tcttctcagt ctgggcaata tgctccacaa gatcggtata
     3421 ctccgcctcg gcaactgacg ctgcgtcaca gatcttcttc caggaaaaca gacgggttcc
     3481 cgcacccgca ccgcggaccg ttccgaagaa tgcaccaaga ttatccgaat cgctcataat
     3541 caatcaggca actatatgtg ccttctatct gataatacat tcgcatcccc caattcggag
     3601 atcaaaccaa aaaaacggaa aatattttca cgggttctga gtcttttcat agaccaagaa
     3661 ccgtgcaacc gtccggacgg cggatgccac ctgcgaagga gtgatcacct gcaccgaaca
     3721 gagcgatgag gcatacagac acttcgtcag atacagcata cactcattcg gcacaaccac
     3781 aaacgcctcc ttacggcatc cgctgggacc aacggtctcg gcaagtttcc gtgcagcatc
     3841 gcggacaaac gtgtctgcct cccgcagccc ctcatacgaa gccggggcaa aagccagaaa
     3901 catcacacgg tgaccgctga tgtcagcaat aaacacatga tgttcgcatg cgggaaccgt
     3961 tccggttgcg tccgcaatac aggaacaggt ctcatccctg tggttcttcc actcctcaag
     4021 gatctcacgc gtaattgcag agtaggaagc aagcgtctgc tgctttgccg agagatctgc
     4081 ctcaagcgaa ctctttgtat c
//
LOCUS       DALTZZ010000073         2533 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_10986,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000073 DALTZZ010000000
VERSION     DALTZZ010000073.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10986"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            112..2379
                     /locus_tag="O0X12_01240"
     CDS             112..2379
                     /locus_tag="O0X12_01240"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330476.1"
                     /note="biodegradative; catalyzes the formation of agmatine
                     from arginine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HJK52817.1"
                     /translation="MEPTDTLSVLIIGADTKSGSAFAHNMQALIDHLKADDMQVISVD
                     SFEGLKDGFIANGAMADRVLIDWNAPSAEELIKLVREQNKYVSIFLLTEPGDLSKIPV
                     EVFPTIDEYAWILGDTPEFIAGRIKSAARKYRKAILPPMFGALMDFSEDFEYSWHTPG
                     HTAGTAFLKSPIGKLFYEFYGEQTFRSDLSISVGELGSLLDHSGPIGAAEKYAAKVFG
                     SDMTFFVTNGTSTSNRVVQGALVVPDEIVFVDRNCHKSMEQAFTLDHGIPVYMVPTRN
                     RYGILGPIPKPEMEPETLAKKLASHPLASKAENQIPVTAVVTNSTYDGVCYNAVAAED
                     LLGQTVDTIHFDEAWYGYAKFNPMYAGKFGMHKDDRPAENRPTVITTHSTHKLLAALS
                     QASMIHIKNGKRPLDHALFNESFMMHASTSPQYSIIASLDVSSKMMDMGGCGLMQEAI
                     DEAVRFRQMMARACGQMNICKPGDWWFRAWQPDEVTMPDGSKVPFAEADPAILAHDPG
                     CWMLRPGEVWHGFTGMTEDWAMLDPIKVTVLTPGMNDDGTHAEFGIPAAVVLAYLGTK
                     GIINEKSGDYNILFLFSMGVTKGKWGTLMSALFDFKTLFDRNAPLEEVLPDLVNAYPD
                     RYGGMTLQTLAAEMHQYIKSSRQTQLANEACAVMPEPVMKPSDAYRYIVKKQVEHVAV
                     DDMANRVVATGVVPYPPGIPMLMPGENAGAADGPILMYLKTLQEFDRKFPGFTHDTHG
                     VEVINGTYYVYCIKE"
BASE COUNT          650 a          789 c          600 g          494 t
ORIGIN      
        1 gatgcatcca aaacatccgg aaaaattgtc accattgata ccgttttatt accgggcaga
       61 caatagttca acacacaaca aacgttgtac caacacaaca ggtgaacaac aatggaaccc
      121 acagacaccc tctccgtcct catcatcggg gccgacacca aatccggcag tgccttcgca
      181 cataacatgc aggcactcat cgatcacctc aaagcagacg acatgcaggt catcagcgtt
      241 gacagctttg aaggactcaa agacggattc atcgcaaacg gtgccatggc agaccgcgtc
      301 ctcatcgact ggaatgcacc aagcgcagaa gaactcatca aactcgtccg tgagcagaac
      361 aaatacgtca gcatattcct cctcaccgaa cccggcgacc tctcaaaaat cccggtcgag
      421 gtcttcccga ccatcgacga atatgcctgg atccttggcg acacccccga gtttattgcg
      481 ggcagaatca agtccgccgc ccggaaatac cgcaaagcaa tcctcccgcc gatgtttggt
      541 gcactcatgg acttctccga agacttcgag tactcctggc acaccccggg ccacaccgca
      601 ggaaccgcat tcctcaaatc cccgatcggc aaactcttct acgaatttta cggagaacag
      661 accttccggt ccgatctctc catctccgta ggcgaacttg gcagccttct tgaccactcc
      721 ggccccatcg gcgctgccga aaaatacgcc gcaaaggtct ttggatccga catgaccttc
      781 tttgtcacca acggaacctc cacctcaaac cgggtagtgc agggagccct cgttgtgccg
      841 gacgagatcg tcttcgtgga cagaaactgt cacaaatcca tggaacaggc cttcaccctt
      901 gatcacggta ttccggttta catggtcccc accagaaacc ggtacggtat cctcggcccg
      961 attccaaaac ccgaaatgga accggaaacc ctcgccaaaa aactcgccag ccacccgctg
     1021 gcctccaaag cagagaacca aatcccggta acggcggttg ttaccaactc aacctacgac
     1081 ggagtctgct acaacgccgt tgcagcagaa gatcttctcg gccagaccgt tgacactatc
     1141 cacttcgacg aagcctggta cggatacgcc aagttcaacc ccatgtatgc aggaaagttc
     1201 ggcatgcaca aagatgaccg ccctgcggag aaccgcccta ccgtcatcac cacccactcc
     1261 acccacaaac tgcttgctgc actctcacag gcatcaatga tccacatcaa aaacggaaaa
     1321 cgtccgcttg accatgcact cttcaacgag tccttcatga tgcatgcatc aacttcaccg
     1381 cagtacagca tcatcgcctc cctcgatgtc tcctccaaga tgatggatat gggcggctgc
     1441 ggactcatgc aggaggcaat cgacgaagca gtgcggttcc ggcagatgat ggcccgtgcc
     1501 tgcggacaga tgaacatctg caaacccggc gactggtggt tccgtgcctg gcagccggac
     1561 gaagtcacca tgccggacgg cagcaaagtc ccgttcgccg aggccgaccc cgcgattctt
     1621 gcacacgatc ccggatgctg gatgctccgg ccgggagagg tctggcatgg ctttacaggc
     1681 atgaccgagg actgggcaat gcttgatccg atcaaagtca ccgtcctgac gccgggtatg
     1741 aacgacgacg gaacccatgc agagtttggt attcccgcag cagttgtcct cgcctatctt
     1801 ggtacgaaag gaatcatcaa cgagaagtcc ggtgattaca acatcctgtt cctcttctca
     1861 atgggtgtca cgaaaggaaa gtggggaaca cttatgtccg ccctcttcga cttcaaaact
     1921 ctcttcgacc gcaacgctcc gcttgaagag gttcttcccg atctcgtcaa cgcatatccc
     1981 gaccgctacg gcggcatgac actccagaca ctcgcagcag agatgcacca gtacatcaaa
     2041 tccagccgcc agacccagct tgcaaacgag gcctgtgccg tgatgccgga acctgtcatg
     2101 aaaccctcgg acgcataccg ctacattgtc aaaaagcagg ttgaacatgt tgccgttgac
     2161 gatatggcaa accgcgttgt ggcaaccggt gttgtcccgt atccgccggg aatcccgatg
     2221 ctcatgccgg gcgaaaacgc cggtgccgct gacggcccga ttctgatgta tcttaagact
     2281 cttcaggagt tcgaccggaa gttccccgga ttcacacacg acacccatgg tgttgaggtg
     2341 atcaacggaa cgtactatgt gtactgtatc aaggagtaac cacccttttt tggagagatg
     2401 gtatggaaaa accagcaacc tcagcacgac cggaaaagat tccggcgatg cagaaagatg
     2461 agtacgacgc cctgatccgc cggggaattc ttgcaaggat tgcattttcc ggagcggaac
     2521 atccgtacat tgc
//
LOCUS       DALTZZ010000074         3960 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_11047,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000074 DALTZZ010000000
VERSION     DALTZZ010000074.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3960)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3960)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3960
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11047"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /locus_tag="O0X12_01245"
     CDS             complement(<1..879)
                     /locus_tag="O0X12_01245"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HJK52818.1"
                     /translation="MEFKPRLVAMPLVVTLLMLIAPVINPYIVGIVVILLSIILYTIH
                     KTHYLAISVAILALLYGIGFIPVIAFLGPLMMMVWGEYLARVLRDYSPETLAFAIGAV
                     LGIVATMFRCMEIDSLVAIIAVVVLLMLRSILVDREDAGMIGLLGVAMTITLFFDLEF
                     TYDATMLALAVVVCAGFAYFAYRAKTVDASGVFAAVLFGIILITFAGVPWFLVVISFF
                     ILGSFFTKYRYAEKVFLGVEQEKSGRRGYMNAFANALVGVAGAILFGLTGNEIFTALF
                     LGCIATATADTLASEIG"
     gene            complement(1001..2104)
                     /locus_tag="O0X12_01250"
     CDS             complement(1001..2104)
                     /locus_tag="O0X12_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HJK52819.1"
                     /translation="MEDVYIARAGALSAKRNTESKIQALCTAAGLETMVSEGDLTAIK
                     VHFGERGNDAFVNALHVAGVIHRVKEIGAKPFLTDTNTLYLGGRRNAVDHIMTALSHG
                     FCFPVTECPVIIADGLKGTNSRDVEVYGKHFDSVKIAADIVAADSMVVVSHFKGHEVA
                     GFGGALKNLGMGCASSEGKREQHTTRPFLKKDSCITCGACINACPEFCISLGGKEIVI
                     DLEHCIGCLMCMNTCPKHAIDIDWKDDGVVFVERMIEYATGAVANKTGKAFYINFLTN
                     ITPHCDCTPWNDIPFVPDIGILASRDPVALDKACYDLVNNAEGVFGSLLPDHHREGEE
                     KFTRVWPGTQPELQFTYAEEMGLGRAEYRLKEI"
     gene            2264..>3960
                     /locus_tag="O0X12_01255"
     CDS             2264..>3960
                     /locus_tag="O0X12_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJK52820.1"
                     /translation="MEDTKTFAEFAISEELLRAIEDMGFEEPTPIQTMAIPQILEGHD
                     VTGQAQTGTGKTAAFGIPIIEGLDPNNKAVQALVLSPTRELAIQTAEEFSRLMKYKQG
                     LSVVPIYGGQPIERQLKVLRGSVQVVIGTPGRVIDHLERGTLNLDSVRMFVLDEADQM
                     LDMGFREDIENIFQHTPEDRQTILFSATMPAPILDITRRFQHDPQFVKITRRELTVPQ
                     IEQTYIEVRERDKLEALCRLLDMNNPELALVFCNTKRTVDDLMARLSARGYFVEALHG
                     DMKQMQRDRVMARFRGGSIDVLIATDVAARGIDVDDVDMVFNYDVPQDVEYYVHRIGR
                     TGRAGRTGKSVTFVAPREIYKLRDIQRYAKIKIAKTPLPSLDDVAEMKMQMFLDKVRA
                     VMTAGNLEKYLPMVEQLLETEDDSEITSIEVAAALLKMHLDTGKNGSAESSETSSSTP
                     QAEPGSFENTGAEPGMVRFRITLGKNQQIRPKDIVGAFAGECNIPGSCIGRIDLYGNY
                     SFVEVPLEHAAVVLETMGQKTIRGQELEIQTATPRAEREEEERERSFRGNDRSGDRKS
                     "
BASE COUNT         1115 a          963 c          971 g          911 t
ORIGIN      
        1 accgatctca ctggcaaggg tatcagcagt tgcggttgca atacatccaa gaaagagtgc
       61 cgtaaatatc tcatttccgg tgaggccgaa gagaattgca ccggcaacgc ccacaagcgc
      121 attggcaaat gcgttcatgt aaccacggcg accacttttc tcctgctcaa cgccgaggaa
      181 gaccttttcc gcataccggt atttggtaaa gaaggatccc agaatgaaaa aactgataac
      241 cacaaggaac cagggcacac ctgcaaacgt aatcaagata atgccaaaca gaaccgctgc
      301 aaaaacgccg cttgcatcaa ctgtttttgc ccggtaggca aagtaggcaa accctgcaca
      361 gacaacaacg gctagagcaa gcatcgttgc atcatacgtg aactccagat caaagaacag
      421 ggtgatggtc attgccacac ctaaaagccc gatcatccct gcatcctcgc gatcgacaag
      481 gatgctacgc aacatgagca gtacaacaac ggcaatgatc gcaaccaggc tatctatctc
      541 catacagcgg aacatggttg caacaatacc aagaaccgca cctatggcaa acgccagtgt
      601 ttccggcgag tagtcccgca gcacgcgggc aagatattct ccccacacca tcatcattag
      661 aggaccaagg aaggcaatga cagggatgaa accaatacca tacagcaggg caagaattgc
      721 aacagaaatt gcaagataat gggttttgtg aatggtatag agaataatgg acagaaggat
      781 gaccactatt ccaacgatat aggggttgat aaccggcgct atgagcatca gcagtgttac
      841 cacaagcggc attgcgacca agcgcggttt gaactccatt gagtgtacat gttagagtac
      901 actctgcaat aaggatgttc gtcgaataga ctttacatcc accaagaagg tttacaaaaa
      961 tcagaagata ccaaaaataa tcggatgtta aagagagacc ttaaatctct tttaaccggt
     1021 attccgctcg gccaagcccc atctcttccg catacgtgaa ctggagctct ggctgtgttc
     1081 ctggccatac acgtgtgaac ttctcctcac cttctcgatg gtgatcaggc agaagacttc
     1141 caaaaacacc ttcagcattg ttgaccagat cataacaggc cttatccagt gcaaccggat
     1201 cgcgggatgc aagaatgccg atatccggca cgaacggaat atcattccat ggtgtacagt
     1261 cacagtgcgg agtaatgttt gtcaggaagt taatgtagaa tgcctttcca gtcttatttg
     1321 caacagctcc tgtagcgtac tcaatcatcc tttccacaaa cacgacccca tcatccttcc
     1381 agtcaatgtc aattgcatgt ttcggacagg tgttcatgca catcaagcag ccgatacagt
     1441 gttcaagatc gataacaatc tcttttccac cgagactaat acaaaactca gggcatgcat
     1501 taatacaagc cccacaggtg atacaggagt ctttcttgag gaacggacga gttgtatgct
     1561 gttcacgttt cccctcactt gaagcacaac ccattccaag atttttgaga gctccaccaa
     1621 atcctgcaac ctcatgacct ttaaagtggg aaaccacaac catgctgtca gcggcaacaa
     1681 tatctgctgc aattttcaca gaatcgaagt gttttccata cacctcaaca tctctggaat
     1741 ttgttccttt gagaccgtcg gcaataatta ccggacactc tgtcactgga aaacagaatc
     1801 catgagaaag tgcagtcata atgtgatcta ccgcatttct gcgtcccccc agatacagag
     1861 tgtttgtgtc ggtcagaaac ggttttgctc cgatctcttt taccctgtga ataacacctg
     1921 caacatgcag ggcattaacg aatgcatcgt ttccacgctc accaaagtgc actttgattg
     1981 cggtaagatc tccctctgaa accatagtct caagacctgc tgcggtacag agagcctgaa
     2041 ttttactttc ggtgttgcgc ttagcagaaa gtgcgcctgc cctggcaatg tagacatcct
     2101 ccatataata atctcattcg tcaggattgc tatttctaat attgggtttt gaaaaaccct
     2161 gacaataggt tcgcaaaaga ggagaccaac acctgactct ttgagaagga ttattccaat
     2221 ttctgggtta ctatatatcc tcagccctcc tatattattg agaatggagg atacgaagac
     2281 attcgcagag ttcgcaattt ctgaagagct tcttcgggca attgaggaca tgggatttga
     2341 ggagccgaca cctatccaga caatggcgat tccgcagatc cttgaggggc acgatgtgac
     2401 agggcaggct cagacgggta cggggaaaac ggcggcgttc gggattccaa ttatcgaggg
     2461 gcttgatcca aataacaaag cggtgcaggc gttagtgctc tcaccaacca gggaacttgc
     2521 aatacagacg gccgaggagt tttcccggct gatgaaatac aaacagggac tgagtgtggt
     2581 tcccatctat ggcggacaac cgatcgagcg ccagctgaag gtgcttcgcg gttctgttca
     2641 ggtggtaatc ggaacacccg gaagggttat tgatcacctt gaacgcggca cactgaatct
     2701 tgattcggtc cggatgttcg tgcttgatga agcagatcag atgctggaca tgggtttccg
     2761 cgaggatatc gagaatattt tccagcacac tccagaagat cgtcagacga ttctgttttc
     2821 agcaacaatg cctgcgccga ttctggacat tacgcgcaga ttccagcatg atccacagtt
     2881 tgtgaagatt acacgccgcg aactgacggt gccgcagatt gagcagacct acattgaggt
     2941 ccgcgaacgt gataagcttg aggcactttg ccggttgctg gatatgaaca acccggagct
     3001 ggcactggtc ttctgtaata caaaacgaac ggttgacgat ctgatggcac gcctgtctgc
     3061 gcgcggatat ttcgtggaag cactgcatgg tgatatgaaa cagatgcagc gcgatcgggt
     3121 gatggcgcgt ttccgcggtg ggtctattga tgtgttgatt gcgacggacg ttgcagcccg
     3181 cggcatcgat gttgatgacg ttgatatggt gttcaactac gacgttccgc aggatgttga
     3241 gtactatgtg cacagaatcg gcagaacagg acgtgcaggc agaaccggaa aatcggtgac
     3301 atttgttgca ccgcgcgaga tctacaaact ccgcgatatt cagcggtatg caaagatcaa
     3361 gattgcaaaa acgcccctgc cgtcactgga tgatgttgca gagatgaaga tgcagatgtt
     3421 ccttgacaaa gtccgtgcag tgatgactgc aggaaacctt gagaagtatc tgccaatggt
     3481 tgagcagctg cttgagactg aggacgattc tgagatcacc agcatcgagg ttgcagcagc
     3541 acttctgaag atgcatcttg ataccggcaa aaacggttcg gcagagagta gtgagacctc
     3601 ttccagtacg ccacaggcag aacctggttc ctttgaaaat acaggtgctg agccaggcat
     3661 ggtcaggttc agaattacgc ttggcaaaaa ccagcagatc cgcccaaagg acattgtagg
     3721 tgcatttgca ggggagtgca atatccctgg aagctgtatc ggacgaatcg atctgtacgg
     3781 caattactcg tttgtcgagg ttcccctgga acatgcagca gttgtccttg agaccatggg
     3841 tcagaaaaca atccgcggcc aggagctgga gattcagact gcaacaccaa gagctgaacg
     3901 tgaggaagag gaacgcgaac gttcattccg cggaaacgat cggtccgggg atcgtaaatc
//
LOCUS       DALTZZ010000075         3746 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_11336,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000075 DALTZZ010000000
VERSION     DALTZZ010000075.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11336"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(100..483)
                     /locus_tag="O0X12_01260"
     CDS             complement(100..483)
                     /locus_tag="O0X12_01260"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HJK52821.1"
                     /translation="MTMQIIPASPAGIEKIAADMKAEGRRRVVITCTAAAEEYDITYS
                     FDKENDLVHYRLTVPEGTVIPSIGASYGGAFVYENEIHDLYGFTFTGMTLDFGGTFIR
                     TKIQYPFKKEKPAPTVTKVTKEGAS"
     gene            complement(480..920)
                     /locus_tag="O0X12_01265"
     CDS             complement(480..920)
                     /locus_tag="O0X12_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HJK52822.1"
                     /translation="MSSAGKSPWLLHYNASSCNGCDIEILAALTPLYDVERFGIINTG
                     NPAHADIFVVTGSVNEQNKEVLKNLYDQIPEPKVVVAVGICASSGGVFRDCYNVSGGV
                     DTVIPVDVYVPGCSVRPEALIEGVVKAIGVLEQKRAVLDSGGSL"
     gene            complement(917..1789)
                     /locus_tag="O0X12_01270"
     CDS             complement(917..1789)
                     /locus_tag="O0X12_01270"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HJK52823.1"
                     /translation="MNLILAATGAVLFLILAPLVGGLIAGVDRVLTARMQSRRGPPVL
                     QPFYDISKLWHKEPAFVNPVEKIFTTAYLVLIAFSGALLFAGGDLLLVVFALALAHTF
                     LILAAYAANAPYSTVGAERELIGVMVAEPVLILLAAGFFMVTGSFFVSDILKEEIPAI
                     IYLPGVFLALFAVLTLKLRKSPFDISTSHHAHQELVKGVTSSLSGKTLAKVEIAHWYE
                     TILILALIFLFFAGNPILGIIVVIICYFLEILMDNLFPRVTWQMTVKSLWIATVILGV
                     INIAVIGFVGGVIG"
     gene            complement(1786..3729)
                     /locus_tag="O0X12_01275"
     CDS             complement(1786..3729)
                     /locus_tag="O0X12_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HJK52824.1"
                     /translation="MSPPELVAILILLLVPLFAAVLLAVLNRDSIRNGIVGVVSAVLI
                     AASLYLAYLAWSSPAPITIALESAKVISVGLFIVELVVALAIIALSLKYRRILPLALA
                     LIQLALLLVLELVYAESVIISTAFTVNNLSAIMVLIIGIIGTLICVYALGYMKDYHHH
                     HTEVADRKPYFFAMLFTFLTAMFGIVLSDSLMWILCFWEVTTLCSFLLIGYSKTPEAV
                     TNSFRAVWMNLIGGIAFTLAIISLLETDPFGATLLSITDIISYHDMAALTIPAALIAI
                     AGLTKAAQMPFSTWLTGAMVAPTPVSALLHSSTMVKAGVYLLVVFAPVFSETAVGLAL
                     AAIGVFTFFVTSALAISQSNAKKVLAYSTIANLGLITACAGIGTPEAVMAAILLIIFH
                     AVAKALLFMCVGAVEHKIDSRDIEDMDGLLVRLPLLATMMVIGICGMYLAPFGMLISK
                     WAAIVAFLEAPFGWAFVILLAFGSAFTIFFWTKWLGKLFMRMNHPQEPDTMLHTSEKI
                     AVTATGVFTILCCIGFAAITVYFVEPYLADIYAGFSETGAMLPDNILMIAIMFVILAV
                     LVLAAHFGSVSGKTLPPYLCGRGVDENGRFHGSLGVYKEAKSSNYYMEEYCGEAILIK
                     PAWIISILLIIVMFVLAFLGVMI"
BASE COUNT         1011 a          905 c          968 g          862 t
ORIGIN      
        1 cctcgaccac atgctcatcc tcgattacga gatcaaggtg aatcggttcg ggcagtaccg
       61 gatgctgcgg cccgaacggt acaacagttc gttttcctgt catgaggctc cctccttcgt
      121 aacttttgtc accgttggtg ctggtttttc cttcttaaac ggatactgta tctttgtacg
      181 gatgaatgtt cctccaaaat caagcgtcat tccggtgaac gtaaatccgt agagatcatg
      241 aatctcgttt tcgtacacaa atgctccccc atatgatgca ccgattgatg gaatcactgt
      301 tccctccgga actgtgagac ggtagtgtac cagatcgttt tccttgtcaa acgagtaggt
      361 gatatcatac tcttcggccg cagcagtgca ggtaatcacc acacgacggc gcccttcggc
      421 tttcatatca gccgcgattt tttcaattcc tgcaggggat gcagggataa tttgcatcgt
      481 catagtgatc ctccgctgtc gagcacagca cgtttttgtt caagaacacc tattgctttg
      541 accactcctt caatgagtgc ttcaggccga accgagcagc ccggtacata tacatccacc
      601 ggaatgaccg tatcaacacc gccggaaacg ttgtaacagt cgcggaacac gcctccggac
      661 gaagcacaga ttcctactgc aacaacaact tttggttctg gtatctgatc gtagaggttt
      721 ttcagcacct ctttgttctg ctcgttcaca ctgccagtca ccacaaaaat atctgcgtgg
      781 gcagggttac cggtattgat aatgccgaac cgttccacat cgtacaacgg cgtcagtgct
      841 gcaaggattt caatatcgca tccgttacag ctggacgcgt tgtagtgcag aagccagggg
      901 gactttcctg ctgagctcat ccaatcacac ctccaacaaa tccgatcact gcaatgttaa
      961 tcacaccaag gataactgtt gcaatccaca gactcttcac tgtcatctgc catgttaccc
     1021 gcgggaagag gttgtccatg agtatttcaa ggaagtagca gatgataact acgataattc
     1081 caagaatcgg atttcctgca aagaacaaaa atatcagtgc aagaatcaga atcgtctcgt
     1141 accagtgcgc aatctccact tttgcaagcg tttttccaga aagtgacgag gtcacacctt
     1201 ttaccagctc ctggtgggcg tggtgagatg tggagatgtc gaacggtgac ttgcgcagtt
     1261 tcagtgtcag tactgcaaag agagcgagga agacacctgg cagatagatg attgccggaa
     1321 tttcttcttt gagaatgtct gagacaaaga agctgcctgt caccatgaaa aatcctgcgg
     1381 caaggaggat caacaccggc tctgcaacca ttacaccgat cagttcacgc tcagcgccca
     1441 ctgttgagta gggggcgttt gccgcatacg ctgcaagaat caggaacgtg tgtgcgagtg
     1501 ccagggcaaa gacgaccagc agcagatcac cgcccgcaaa cagcagtgcg ccggagaacg
     1561 caatcagtac cagataggct gtggtgaaga ttttttccac cggatttacg aatgcaggtt
     1621 ctttgtgcca gagtttgctg atatcataga acggctgcaa aaccggaggg ccgcgccggc
     1681 tctgcatccg tgctgtaagt acgcggtcaa ctcctgcaat cagtccgccg actagcggag
     1741 caagaatcag gaacagaacc gcaccggttg ctgcaagtat cagattcata tcatcacccc
     1801 cagaaaggcg agtacaaaca tgacaatgat gaggagaatg gagatgatcc acgcaggttt
     1861 gatcagtatg gcttccccac agtactcttc catatagtag ttcgaggatt ttgcttcttt
     1921 gtacacacca agtgatccat ggaatcttcc gttctcatca actccgcgac cgcaaaggta
     1981 cggcgggagt gttttaccgg aaacagatcc aaagtgtgct gcaagtacga ggactgcaag
     2041 gatcacaaac atgatagcaa tcatgagaat attatcggga agcattgctc cggtttctga
     2101 aaatcctgca tagatatctg cgagatatgg ctcgacaaag tacactgtaa ttgcagcaaa
     2161 gccgatgcag cagagaattg taaagactcc tgtggcagtt accgcaatct tttcagatgt
     2221 gtgcagcatg gtatccggtt cctgcgggtg gttcatgcgc atgaacagtt ttccaagcca
     2281 cttcgtccaa aagaagattg taaatgcact gccgaatgca aggaggatga caaatgccca
     2341 gccgaacggc gcttccagga acgcaacaat cgctgcccat ttggagatga gcataccgaa
     2401 tggtgcaaga tacatgccgc agataccgat gaccatcatg gttgccagca gcgggagccg
     2461 tacaagcagt ccgtccatgt cttcaatgtc acggctgtcg attttgtgtt caactgcacc
     2521 gacacacata aacaggagtg cttttgcaac cgcgtggaag attatcagga gaatcgctgc
     2581 catgacagcc tctggtgttc cgattccggc acaagcagtg atcaggccga ggtttgcaat
     2641 tgtagagtat gcgagtactt tctttgcgtt actctgcgag atggcaagtg ctgatgtaac
     2701 aaagaaggta aagacaccta ttgctgcaag ggcaaggccg acagcggtct ctgagaagac
     2761 aggtgcgaac acgaccagca gatagacacc ggcttttacc atagtacttg agtgcagcag
     2821 agcggagacc ggcgtcgggg caaccattgc accggtgagc catgttgaga acggcatctg
     2881 cgctgctttt gtaagaccag caatggcgat aagtgctgca ggaatggtga gggctgccat
     2941 gtcatggtag ctaataatgt ctgtgatgct gaggagtgtt gccccaaacg gatcagtttc
     3001 caggagagag atgatcgcaa gcgtgaatgc aatccctccg atgaggttca tccataccgc
     3061 acggaatgag ttggtgaccg cctcgggtgt ctttgagtat ccaatgagga ggaatgaaca
     3121 gagtgttgtc acttcccaga agcagaggat ccacatgaga ctatctgaga gaacgattcc
     3181 aaacattgct gtcaggaagg taaagagcat tgcaaagaag tacggcttgc ggtctgcaac
     3241 ttctgtatga tggtggtgat agtctttcat gtagccgagg gcatacacac aaatgagtgt
     3301 tccaatgatg ccgatgatca gcaccatgat ggctgacaaa ttgttcacgg tgaatgctgt
     3361 tgagatgatg acggactcgg catagaccaa ctccagaaca agcagcaggg caagctggat
     3421 tagggcgaga gcaagcggaa gaatccgccg gtatttgaga gaaagtgcga tgattgcaag
     3481 ggcaaccacg agctctacga tgaataggcc gacgcttatc actttggcag attccagggc
     3541 aatcgttatc ggggcaggac ttgaccatgc aagataggca aggtagaggg atgccgcaat
     3601 aagaactgcc gaaacaacac cgacgatacc gttgcgaatg gagtccctgt tcaataccgc
     3661 aagaagaact gcggcaaaca ggggaaccag cagcagtatg aggatggcta cgagttccgg
     3721 cggggacata atatcacttg tttaaa
//
LOCUS       DALTZZ010000076         3007 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_11405,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000076 DALTZZ010000000
VERSION     DALTZZ010000076.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11405"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="O0X12_01280"
     CDS             <1..671
                     /locus_tag="O0X12_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HJK52825.1"
                     /translation="LALLIGQILLILILVWIVFVLGMVVLVVLSIKRKHLYFPILLRP
                     VLAFTEGAVATGCQILGVDSGQLMEFLIRIDNDVNTTAFAAVPVENRVVFFPHCLRSA
                     KCPARLTPDGLKCITCGRCGLGSVVPPLEEAGYQTFIIPGSTFVKRMVRKYRPQAMIG
                     VGCIVEVKEGLELGKRIHMITLGVVTKTDGCVETTMDWDELLEVASIGLPEPIVRRET
                     ERKE"
     gene            complement(699..1454)
                     /locus_tag="O0X12_01285"
     CDS             complement(699..1454)
                     /locus_tag="O0X12_01285"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52826.1"
                     /translation="MTEKLIDRLVKFGFTLYEARVYVSLISIRVGSARDIQELTGIPR
                     GRVYDVLESLVQKGYAGVTKGTPTLYSATDVQETFDRLKIDHSRMCDSLAADLIKIEQ
                     KVTAKTLTFQSYELHTEWALENQIQSLFRRCRSEMVVLSTNPQTIQKYETDLKKLAKR
                     VQLYVIVDDPARFTGVSLKLYTGDQDIDTIFLGTKAPDGITDRVPDLMIFSDEKETLS
                     VVRSGDAIGGIMVTNGIFGKYFQKSILRHLNPV"
     gene            1554..2777
                     /locus_tag="O0X12_01290"
     CDS             1554..2777
                     /locus_tag="O0X12_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK52827.1"
                     /translation="MSGSDKPFVAGKATMIVLLLAAMPALLGTAAVAPALPLISEAFP
                     DASETLISLIITLPPLATALSGFLIGAISDRYGRKRVLITSLALFGLAGSSGFFLDSV
                     TSIIFWRLWLGVGLAGLLPTVTTLLTEYYDGPTRARYMGYLSAAMGVGGLVLQTGCGL
                     LAEISWREPFLIYVFGLLVIPLVLIYVKEPKRLTDTEEFNDNLEMPRSRIKKLDTKPV
                     LLVYVTLFVSLIMMYLISSKIAYLLTQTADVSTTICGLILGMAGLFSAVSSYSFWRFA
                     RRFTPMQMFSLMFFFEGAGLFIVGTAINVPVIALGAAMVGFGLGLGTPTGSMWLSAIT
                     PQKYLGRIMGGQTVAVYFGVFASSFVGAELLAMTGSYQGLFLTIGVVGLAVGVVYWVA
                     GTLQKRVKGSKTGSV"
     gene            2812..>3007
                     /locus_tag="O0X12_01295"
     CDS             2812..>3007
                     /locus_tag="O0X12_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52828.1"
                     /translation="MQFGKLAAIVCVVAVVCLAVTAGCAVAAPSDAPQQGCVPYHHAY
                     LSCPYDPGQCPADCGWSEPRY"
BASE COUNT          533 a          653 c          967 g          854 t
ORIGIN      
        1 atcttgcact tttgatcgga cagattcttc tgattctgat tcttgtctgg attgtgtttg
       61 tgctggggat ggtggtgctg gttgtgcttt ccattaagcg gaagcatctg tacttcccga
      121 ttcttctccg gccggttctt gcgtttacgg aaggggcggt tgcaaccggc tgtcagattc
      181 tcggagtgga cagcgggcag ttgatggagt ttttgatccg gatcgataat gatgtgaaca
      241 cgactgcgtt tgcagcggtg ccggtggaga accgtgtggt gtttttcccg cactgtctgc
      301 ggtcggcaaa gtgtccggca cggctgacgc cggacgggct gaagtgtatt acctgcggga
      361 gatgtggact cgggtcggtt gttccgccgc tggaggaggc cgggtatcag acgtttatta
      421 ttccgggttc gacgtttgtg aagcggatgg tgcggaagta ccggcctcag gcgatgatcg
      481 gggttggctg tattgttgag gtaaaagagg gactggaact tggaaagcgg attcacatga
      541 ttacgcttgg agtggtgaca aagacggacg gctgtgtgga gacgacgatg gactgggatg
      601 agctgctgga ggttgcgtcg atcgggttac ccgagccgat cgtcagaagg gaaacggaga
      661 ggaaggagta aaaaagattt ttttccggtt ggtccgtctc agaccggatt cagatgccgg
      721 aggatgcttt tctggaagta tttcccaaaa atgccgtttg tcaccatgat gccgccgatg
      781 gcatcaccgg atctgacgac ggagagtgtt tctttttcat cggagaagat catcagatcc
      841 ggtacacgat ccgttatgcc gtccggggct tttgttccaa gaaagatggt gtcgatgtcc
      901 tgatcacctg tgtagagttt cagtgagacg ccggtaaacc gtgcgggatc gtcgacgatt
      961 acgtagagct gtacccgttt tgccagtttt tttaagtcag tctcatactt ctgaatggtt
     1021 tggggatttg tggataatac caccatctca ctgcggcaac gccggaacag ggactgaatc
     1081 tggttttcca gtgcccattc ggtgtgcagt tcgtaggact ggaaggtgag ggttttggcg
     1141 gtcaccttct gttcgatttt gatgaggtcg gcggcgagcg agtcacacat gcgggagtgg
     1201 tcgatcttga gccggtcaaa ggtttcctgt acatctgttg cggagtagag ggtaggggtt
     1261 cctttggtga cgcccgcata gcctttctgg acgaggcttt cgaggacgtc atagacgcgg
     1321 ccgcggggga tgccggtaag ttcctggatg tcgcgggcgg atccgacgcg gattgagatc
     1381 aggctgacgt atacgcgggc ttcatacagg gtgaacccga actttaccag ccggtcgatg
     1441 agcttttccg tcatttccag agagtttgct gtactcctat acacagttta cgtctttgca
     1501 actctgttca gggacaccat attcatatta tctcagtgtg aaccagatac cacatgtctg
     1561 gttccgataa accatttgtt gcgggtaagg caacgatgat tgttctgctg cttgcggcaa
     1621 tgccggctct gcttggtact gctgcggtgg caccggcgct tccgctgatc agcgaggcat
     1681 tcccggacgc gtccgagacg ctgatttcgc tgatcattac cctgccgccg cttgcaacag
     1741 cattgtcggg atttcttatc ggcgcaattt cggatcggta tggcagaaag cgcgtactga
     1801 tcacaagtct tgctttgttt ggtcttgcag gttcgtcagg atttttcctg gactcggtga
     1861 cgtcgatcat cttctggcgg ctgtggcttg gtgtgggtct tgccggtctg ctgccgacgg
     1921 ttacgacact tctgacggag tactacgacg gaccaacccg tgcccggtat atggggtatc
     1981 tgtctgctgc gatgggggtc ggcggtctgg ttcttcagac gggctgcggt cttcttgcgg
     2041 agatttcgtg gcgtgagccg ttcctgattt atgtgttcgg gcttttggtt attccgctgg
     2101 ttctgatcta tgtgaaagag ccgaaacggc tgacggatac ggaggagttt aatgataatc
     2161 tggagatgcc aagatcccgg attaagaagc tggatacaaa accggttctg ctggtgtatg
     2221 tgacgctgtt tgtgtcactg attatgatgt atctgatctc gtcgaagatt gcgtatcttc
     2281 tgacgcagac ggcggatgtg tcgacgacga tctgcggcct gatccttggt atggcgggtc
     2341 ttttcagtgc ggtttcgagt tattcgttct ggcggtttgc ccgcaggttt acgccgatgc
     2401 agatgttttc cctgatgttc ttcttcgagg gagccgggct tttcatcgtg ggtacggcga
     2461 ttaatgtgcc ggttattgcg ttaggtgctg cgatggtggg ctttggtctt ggtcttggta
     2521 ctccgaccgg ttcgatgtgg ctgtcggcaa ttacgccgca gaagtatctc ggccgtatta
     2581 tgggtggtca gacggtggcg gtgtactttg gtgtgtttgc gtcttcgttt gtgggagcag
     2641 aacttcttgc aatgaccggc agctatcagg gtctgttcct gacgattggt gttgtgggtc
     2701 ttgcggtcgg tgtggtgtac tgggttgcag gtacgctgca aaagcgggta aaagggtcaa
     2761 agacgggctc cgtctgatcc cttgatattt tttcaggtgg aatagatctg tatgcagttt
     2821 ggaaagttag cggcgatcgt ttgtgttgtg gcggttgtct gtcttgcggt tacggccggg
     2881 tgtgcggttg ctgctccgtc ggatgcaccg cagcagggtt gtgtgccgta tcatcacgcg
     2941 tatctgtcgt gcccctatga tccggggcag tgtccggctg actgcggatg gagtgagccg
     3001 cggtacg
//
LOCUS       DALTZZ010000077         2781 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_11416,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000077 DALTZZ010000000
VERSION     DALTZZ010000077.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11416"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="O0X12_01300"
     CDS             complement(<1..1332)
                     /locus_tag="O0X12_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HJK52829.1"
                     /translation="MYKSIDDINERIHDGSVRVVTAEEMPDIVDELGVSGALREVDVV
                     TTGTFGAMCSSGAFLNFGHPEIPIRMEKMWLNNVEAYAGIAAVDCYLGATQESITRGT
                     EYGGAHVIEDLIAGRSVELHARAKGTDCYPRQSITNEFTLSDLNQAIMVNPRNSYQCY
                     NAATNMGDHAIKTYMGYLLPGHRNVTYSGAGALSPLPNDPTFSVIGSGTPIFLGGANG
                     TIIGEGTQHSPGAGFGTLMTTGDMKEMSTDYIRAATMTGYGVTMYVGVGVPIPLLNEE
                     IVKHTAVRDEDLVTSIVDYGTPVRDRPVVRDVTYAELKSGSVEINGEEIRTSSLSSYR
                     KAREVANELKNRIEAGTFTMALATRPINATKKNKPMAEHGVVRRVSEMMVSTFVTITG
                     EESVKEAANRLLKGNTNHLPVIDSENRLIGIVTTFDISKAFACDEEDQKVSE"
     gene            1400..2107
                     /locus_tag="O0X12_01305"
     CDS             1400..2107
                     /locus_tag="O0X12_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HJK52830.1"
                     /translation="MYIGEFIQKLEAIAPPALAEEFDTGRIGLILEGTANIETVACAL
                     DATPHVAEMAAELEVDALVVHHPAFWVPMHGICGRNADVARPLLANNINLYAMHTNFD
                     HADSGINDALADLLGLTDRVKGSLEIIGTMTKPFPAIAEILGCSLRVWGDISEVTRLA
                     VAGGSAFDWELIEEAKDLGAEAYLASELKYNIARESPLPCIEATHYALEAPGMRALAQ
                     REGWIFIEDIPETSIIP"
     gene            2104..2451
                     /locus_tag="O0X12_01310"
     CDS             2104..2451
                     /locus_tag="O0X12_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52831.1"
                     /translation="MIHPWKVLKTEASLNETVRQAFIHAYGKRGSDAVDAVRDHRVKK
                     YRDYFVVVGNSGEYYVEGDFCSCEARLYGKTCWHTLAVRIAQEVGEYETYNLWYYKDG
                     VDEEEPEYTGEQL"
     gene            2509..>2781
                     /locus_tag="O0X12_01315"
     CDS             2509..>2781
                     /locus_tag="O0X12_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52832.1"
                     /translation="MQEDTYYEDMASILITCYSRTGKTKLLAEAIGRGAAEVPGIKVD
                     VLAFNRVTASDFVRYDGIILGSPVYFGGVASEFKELIDDTTFVRGRL"
BASE COUNT          693 a          719 c          677 g          692 t
ORIGIN      
        1 ctctgatact ttctgatcct cctcatcgca ggcgaatgct ttggagatgt caaatgtcgt
       61 cacaatacct atcaaccggt tctcggagtc gatcaccggc aggtgattcg tgtttccttt
      121 cagcagacgg ttcgccgcct ccttaacact ctcctcaccg gtgatggtca caaacgtact
      181 gaccatcatc tcgctcacgc ggcggacaac tccatgttcg gccatcggtt tgtttttctt
      241 cgttgcattg atcgggcggg ttgcaagtgc catcgtaaac gtacccgcct caattctgtt
      301 tttcagttcg tttgcaacct cacgtgcttt tctgtagctt gacagagaag atgtcctgat
      361 ctcctctccg ttaatctcaa ccgaaccgct cttcagttct gcgtatgtca catcacgtac
      421 caccggccga tcacggaccg gggtaccata gtcaacgatg ctggttacaa gatcctcatc
      481 ccgtaccgca gtgtgtttca caatctcttc atttagcaac gggattggaa cccccacacc
      541 gacgtacatt gtcacaccat atccggtcat cgttgccgcg cgaatatagt ccgttgacat
      601 ctctttcata tcaccagttg tcatcagtgt cccaaaccca gcaccaggcg agtgctgagt
      661 accctcaccg atgatcgttc cgttcgcacc tcccaggaag ataggtgttc ctgaaccaat
      721 caccgagaat gtaggatcgt tgggaagagg ggatagagct ccagcaccag agtaggtaac
      781 attacggtga ccgggcagca gatatcccat ataagtcttg atcgcatggt ctcccatgtt
      841 tgttgctgcg ttgtagcact ggtaggagtt ccgcgggttg accataatgg cctgattgag
      901 atcggataat gtgaactcat tggtgatgga ctgacgtgga taacagtctg tacccttggc
      961 acgtgcatgg agttcaactg atcgacccgc aatcagatct tcgattacgt gtgccccgcc
     1021 gtactcggta ccacgggtta ttgactcctg agttgcccca agataacagt caaccgctgc
     1081 aatacctgca tatgcctcaa cgttattgag ccacatcttc tccattctga tggggatttc
     1141 aggatgtccg aagttgagga acgccccaga tgaacacatc gcgccgaagg tgccggttgt
     1201 gacaacatcc acttcccgga gtgctccgga cacgccaagt tcatcaacga tatccggcat
     1261 ctcttcggct gttacaaccc gcacacttcc atcgtgaata cgttcgttaa tatcatcgat
     1321 ggatttgtac atatagccta atatctccct gttccaggta tatatgactt attaatcccg
     1381 caaagccaat atctatctaa tgtacatagg tgagttcatc caaaaacttg aggccattgc
     1441 ccctcctgcc cttgcagaag aatttgacac cggtcgtatt ggtctgattc ttgaaggaac
     1501 agcaaacatt gagacagttg cctgtgccct ggatgcaaca ccccatgttg cagagatggc
     1561 agcagaactc gaggttgatg cgctggtggt acatcatcct gcattctggg taccaatgca
     1621 tggaatctgt ggcaggaacg cagatgttgc cagaccactt cttgcaaata acatcaacct
     1681 ctatgcgatg cacacgaatt ttgatcatgc agacagcggt atcaatgatg cactggcaga
     1741 tctgctcggt ctgaccgacc gcgtgaaggg atcacttgag atcatcggca ctatgacaaa
     1801 accgttcccc gcaatagcag agattctcgg atgtagcctt cgcgtctggg gcgatataag
     1861 cgaggtaacc cgactcgcgg ttgctggcgg atcagccttt gactgggaac tgatcgaaga
     1921 ggcaaaggat ctcggtgccg aagcatatct tgcatccgaa ctgaagtaca acattgcccg
     1981 ggaatcacca ctcccctgca ttgaggcaac ccattacgca ttggaagccc ccggcatgcg
     2041 tgcactggca caaagagagg gctggatctt cattgaggat attcccgaga catctataat
     2101 tccatgattc acccctggaa agttctgaaa acagaggcat ctctcaatga gacggtccga
     2161 caggcattta tacatgctta cggaaaacgc ggaagtgatg cggttgatgc ggtccgtgac
     2221 caccgcgtga aaaaatatcg ggactacttt gtggtagtgg gaaactctgg agaatattac
     2281 gtagaaggag atttctgcag ctgcgaggcg cggttgtatg gaaaaacctg ctggcacacc
     2341 cttgcagtac ggattgcaca ggaagttggt gagtatgaga catataatct ctggtactac
     2401 aaggatggtg ttgacgaaga ggagcctgag tatacaggag aacaactatg atacagagga
     2461 aattaaacca ggttgtccta acagataccc aatgccgacc tttaagccat gcaggaagat
     2521 acatattatg aagacatggc atcgatactt atcacctgct acagccgcac gggcaaaaca
     2581 aaactacttg ccgaggccat tggccgcggt gccgcagagg ttcccggcat aaaggttgac
     2641 gtacttgcat ttaaccgcgt gactgcaagc gattttgtcc ggtatgatgg aattatcctt
     2701 ggatcacccg tgtactttgg cggggtcgca tctgagttca aagagcttat cgatgatacg
     2761 acattcgttc gcggcaggct c
//
LOCUS       DALTZZ010000078         6297 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_11452,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000078 DALTZZ010000000
VERSION     DALTZZ010000078.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6297)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6297)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6297
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11452"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="O0X12_01320"
     CDS             complement(<1..270)
                     /locus_tag="O0X12_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HJK52833.1"
                     /translation="MTDQEIFSDLSRIGKRLFTEHLVGGNFGNMSARYADGYFITRTG
                     SYLDADPAQVVLMPLNGRVTPGASSEWRVHTAIYNESEQHAAIVHA"
     gene            311..979
                     /locus_tag="O0X12_01325"
     CDS             311..979
                     /locus_tag="O0X12_01325"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HJK52834.1"
                     /translation="MDEYSEVVLWLNDHANSHNAEGMRRFGISGGKILGISVVNLRKY
                     AKTLGKDQGLSVFLWNTGIHEARILASMTAVPACADDALLESWVLDLDSWDVCDQVCK
                     NFFAHCNGTQERAVRWCSREEEFVRRAGFVLICEMAIHHKDMPDEAFLLYLDLIRRYS
                     TDDRNFVKKAVNWALREIGKRRHSISRECLDLSDELAVSENPTARWIGRNAGQELRDK
                     MKKK"
     gene            complement(980..1897)
                     /gene="argF"
                     /locus_tag="O0X12_01330"
     CDS             complement(980..1897)
                     /gene="argF"
                     /locus_tag="O0X12_01330"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HJK52835.1"
                     /translation="MKKDFISITDLSCEEYDDILTLAARLKRQRYAGVPHPLLAGKTL
                     AMIFEKASTRTRISFDVGMYDLGGYALYLNSRDTQLGRGETIADTARVMSRYVHGAIM
                     RTYKHETITEFAKYASIPVINALSDKEHPCQILADSLTLKEKFGDLNGLRVAWIGDGN
                     NVCNSLIMATVQTGMEIAVATPKGYEPDPAAIRFARENGGKIVEYDDPKKAVEDAHAI
                     YTDTWISMGEEDIKDTKLKDFNGFCLDMPLLKRAAPDAIVLHCLPAHRGEEITDEVID
                     SMQSGVWDQAENRLHAQKAVLVRLMGQNL"
     gene            complement(1894..3192)
                     /gene="purD"
                     /locus_tag="O0X12_01335"
     CDS             complement(1894..3192)
                     /gene="purD"
                     /locus_tag="O0X12_01335"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HJK52836.1"
                     /translation="MNMRILVVGGGGREHAITLALSKNTKTDLYCVMGKKNPGIAKLC
                     REVLIHPETDADAVLAFAKEHNIDYAVVGPEAPLAAGVSDALVAAGIGCVGPSKAAAR
                     IETDKGFCRSLMQKYGIDGCPAYKLCKNPEEAAEYIRSYTGDLAVKPTGLTGGKGVKV
                     MGEQVDREGAVAYAMTLRDQTIILEERLLGEEFTLMAFVDGKTLVPMPLVQDHKRAFD
                     GDVGPNTGGMGSYTLADHTFPFVTEADYNAAFAIMQETVAALAKEGCPYKGVLYGQFM
                     NTADGPKVIEFNARFGDPEAMNVLTLLDSDFVTIAEHIVAGTLTPDDVSFKKQATVCK
                     YIVPKDYPEHPHAGDPITLGSHENTILYYANVVEENGVLQTQTSRTMAFVGVGADLAE
                     AEIAAETACRNVSGNVRHRADIGTEALFAKRIAHMKELRG"
     gene            complement(3218..3730)
                     /gene="pyrE"
                     /locus_tag="O0X12_01340"
     CDS             complement(3218..3730)
                     /gene="pyrE"
                     /locus_tag="O0X12_01340"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HJK52837.1"
                     /translation="MENPVLDLLIQYKAVEFGDFTLASGVKSKYYIDVKTAIMQPALL
                     GEIAKEVAERYTFDAVAGVAVGGVPLAVAVSLASGKPCAVIRAAAKDHGKSQMVIGDV
                     AGKHVLLIEDVTTSGGSSKYGIDELRKAGAIVDSVVVVVDRDSGAEALLAAEGVTLHP
                     LVRASELLNR"
     gene            complement(3880..4437)
                     /locus_tag="O0X12_01345"
     CDS             complement(3880..4437)
                     /locus_tag="O0X12_01345"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HJK52838.1"
                     /translation="MIVETILAVVTTFVAAFWIMVPAYVANAGAVPFGGGTPVDFGKC
                     AKDGRRYLGDGKTWRGLIGGIVVGILFGLLQIFLAQTFHWDWLPEQTIVTITALAAGA
                     LLGDMVKSFFKRRAGKDRGEKWPIADMYDLVAGSLVLLAVFAWPWIAANMTIWIFIAI
                     LIWTPLLHRGANIIGYLIGIKQVPW"
     gene            4539..6080
                     /locus_tag="O0X12_01350"
     CDS             4539..6080
                     /locus_tag="O0X12_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJK52839.1"
                     /translation="MKIIKETKGMCPVCGKLLPATVTEEDGAIWIRRTCPDHGEFRSL
                     YWSDAEMYHRFEQYDREGRGVYNPNTNTPGECTQRCGLCSHHKSGTLLANIDLTNRCN
                     LNCEFCFANARACGYIYEPTFDEIVSMLSLLRNEEPVPCPAVQFAGGEPTMRDDLVEI
                     IKKAKEFGFSQVQMASNGIKLARNPDLAKELREAGLSTVYLHFDGTTKETNPLLEKTS
                     LPAIENCRKAGLGIVLVPTVINGKNDHQIGDIIRFAAKNIDVIRGINFQPVAFTGAAK
                     NDDVERERVTVPDLVERMEEQTGGALRRTDFYPVPSMMSVCDLIESYTGEAQIMFCAH
                     PHCGAATYGFVNEKGDIVPVNRFIDVDKFLSEVAKMAEKFRSAGKTGRYLAMVTGINN
                     MRKIVEKGEVEGIKIDMNKMIYNALIKHDYNSIGQFHKNALFIGTMHFMDCFNYDTDR
                     VERCCIHYATPDGRLIPFCTYNSGPVYREQVWKKYAKPMPAKKNASDTSCAGCDACGS
                     VDTTE"
BASE COUNT         1529 a         1618 c         1519 g         1631 t
ORIGIN      
        1 ggcatgaacg attgctgcat gctgttccga ttcgttgtag attgctgtgt gtacccgcca
       61 ctcactggat gctcccggag taactctgcc gtttaacggc atgagaacta cctgtgcagg
      121 atctgcatcg aggtaggaac cggtccgtgt aataaaatag ccgtcagcgt agcgtgcgct
      181 catatttcca aagtttccgc cgacgagatg ttcggtaaat agtcgttttc cgatgcggga
      241 gagatcggaa aagatttcct gatcagtcat atgcggaata tatttgctgt attctcctaa
      301 agatactggt atggatgagt actccgaggt tgttctgtgg ctcaacgatc atgccaattc
      361 ccacaatgcc gagggtatga gacgctttgg tatctccggc gggaagattc ttggaatttc
      421 ggttgtaaac ctgcggaagt atgccaaaac acttggaaaa gatcagggac tttctgtttt
      481 cctctggaat acagggattc acgaagcaag aattcttgcg tccatgacag ctgttccggc
      541 atgtgcagat gatgcccttc ttgagtcatg ggttttggat ctcgattcat gggatgtctg
      601 tgatcaggtc tgcaagaact tctttgcaca ctgcaacgga actcaggaac gggccgtacg
      661 ctggtgcagc cgtgaggaag agttcgttcg acgtgcaggt tttgttctca tctgcgagat
      721 ggctattcac cacaaagata tgccggatga agcttttctt ctgtatctgg atctgattcg
      781 ccggtactca accgacgatc ggaattttgt aaaaaaagcg gtcaactggg cactgcgtga
      841 aattggaaaa cgcaggcaca gcatcagcag agagtgtctg gatctttctg atgaactggc
      901 cgtctcggag aatcccactg cacgatggat tggaagaaat gccgggcagg aactcaggga
      961 taaaatgaag aaaaaatgat tacagattct gtcccatcag tcggacaaga actgcttttt
     1021 gtgcatgcag acggttctct gcctgatccc agactccgct ctgcatggag tcgataacct
     1081 cgtctgtaat ctcctctccg cggtgggcag gcaaacaatg cagaacaatt gcatccggag
     1141 cagcgcgttt gaggagcggc atatcaagac agaatccgtt gaagtccttc agcttggtgt
     1201 ccttgatgtc ctcctcgccc atggaaatcc atgtgtccgt gtagatagcg tgtgcgtcct
     1261 ctactgcctt ctttggatca tcatactcca caatctttcc gccgttctcc cgtgcaaacc
     1321 ggattgcagc cggatccggt tcgtaaccct tcggtgttgc aaccgcaatt tccattcccg
     1381 tttggaccgt tgccataatc agagagttac agacattgtt gccgtcaccg atccaggcaa
     1441 cccgcagacc gttgagatcc ccaaacttct ccttcagagt aagagaatct gcaagaatct
     1501 ggcacgggtg ttccttgtcg gacagtgcat tgatcaccgg aatggatgca tactttgcaa
     1561 actccgtaat cgtctcatgt ttgtatgtcc gcattattgc accatggaca tatcgggaca
     1621 tcactcgtgc ggtatccgct atcgtctcac cacgcccgag ctgagtatct cgtgagttca
     1681 ggtacagcgc atatccgcca agatcataca tgcctacgtc aaaggagatg cgggtccgtg
     1741 ttgatgcttt ctcaaagatc attgccagcg tttttcctgc gagcagcggg tgaggcactc
     1801 ccgcataccg ctggcgtttc agccgtgcag cgagtgtaag gatgtcgtcg tattcttcgc
     1861 acgagagatc cgtaattgag ataaagtcct ttttcatccc cgcagctcct tcatgtgcgc
     1921 aattcttttt gcaaacagtg cctctgtccc gatatctgcg cggtgacgga cattgccgga
     1981 cacattcctg catgcggtct ctgctgcgat ttccgcctcg gcaagatcgg caccgactcc
     2041 gacaaatgcc atagtccggg atgtctgtgt ctgcagtact ccgttctcct ccaccacgtt
     2101 tgcatagtag agaatcgtat tttcgtgtga tccaagtgtg atcggatcgc ctgcatgcgg
     2161 atgctcgggg tagtcttttg gtacaatgta cttgcagacc gttgcctgtt ttttgaagga
     2221 gacatcatcc ggggtgagcg ttccggctac gatatgttct gcaatggtaa caaagtcaga
     2281 gtccagaagc gtcaacacat tcattgcttc cggatcccca aacctggcat tgaactcaat
     2341 gacttttggt ccgtctgctg tattcataaa ctgcccgtac agtactcctt tgtaaggaca
     2401 accttccttt gcaagtgctg caactgtctc ctgcatgatt gcaaacgctg cgttgtagtc
     2461 cgcttcggtt acaaacggga aggtgtgatc tgcaagcgta tacgatccca tcccgccggt
     2521 gttcgggccg acatccccgt caaacgcacg cttgtgatcc tgcacaagag gcatggggac
     2581 cagtgtcttt ccatccacaa acgccattaa cgtaaactcc tcaccaagaa gccgttcttc
     2641 cagaatgatc gtctgatccc tgagcgtcat cgcataagcc actgcccctt cgcggtcaac
     2701 ctgctcgccc atcaccttga ctcccttgcc gccggtaaga ccggttggct ttaccgcaag
     2761 atcccctgta taggatctga tatactcagc agcctcttcg ggatttttgc agagtttgta
     2821 tgcaggacag ccgtcaattc catatttctg catcagactt ctgcaaaatc ctttgtcagt
     2881 ctcaatgcgt gcggctgcct ttgacggacc tacacatccg atacctgctg caaccagtgc
     2941 atcagagaca cctgccgcga gcggggcttc aggacccaca accgcataat caatgttgtg
     3001 ctcttttgca aatgcgagca cagcatcggc atctgtttcg ggatgaatca gtacttcgcg
     3061 gcagagcttt gcgattcctg ggttcttctt acccattacg cagtacagat ctgtttttgt
     3121 attcttcgag agtgcgagtg tgattgcgtg ttcccgtcct cctccaccca caacaagtat
     3181 tctcatgttc atatctgtct cctgttttgc aaataattca gcggtttaac agctcacttg
     3241 ctctgaccag aggatgcaga gttacgccct ctgctgcaag cagtgcttcc gccccgctgt
     3301 cacgatcgac gacaacgaca actgagtcaa caatagcgcc cgctttgcgc agctcatcaa
     3361 ttccatactt tgaggaacca cccgatgttg ttacatcctc aattaagagg acatgctttc
     3421 cggcaacatc accgatgacc atctggcttt ttccgtgatc ctttgctgcg gcacggatga
     3481 cagcacaggg ttttccgctt gcaagcgata ctgcaaccgc gagcggtact cctcccacgg
     3541 caacaccggc aacagcatca aatgtgtagc gttcggcaac ctcttttgca atctcgccaa
     3601 gcagtgcagg ctgcatgatt gctgtcttta catcgatgta atatttgctt tttacaccgg
     3661 aagcaagagt aaaatctccg aactccacgg ctttatattg gattaacaga tctaataccg
     3721 gattttccat gatctccgca aaactcctga tgaaatattg tgtactatag gaggttgaag
     3781 aacattaaat attcccagga ttttctggta tcgttttcta catctcttga tctgtgggtg
     3841 ctcttacctc tgtaaaatat ggataaccgg aaattccggt taccagggaa cctgtttgat
     3901 acctatcagg tatccgataa tgtttgcccc gcggtggaga agcggtgtcc agatgagaat
     3961 tgcaataaag atccagatgg tcatgtttgc agcaatccac ggccaggcaa agactgcaag
     4021 aagcacgagg gatcctgcga ccagatcgta catatccgca attggccact tttccccgcg
     4081 atcttttcct gcgcggcgtt taaagaaact ttttaccatg tctccaagga gcgcacctgc
     4141 ggcaagggcg gttatggtta caatggtctg ttccggcaac cagtcccagt gaaatgtctg
     4201 tgcaagaaat atctgcagaa gaccaaacag aattccaaca acgattcctc cgatgagtcc
     4261 gcgccatgtt tttccgtccc caagatatct gcgtccgtct tttgcacatt ttccaaagtc
     4321 caccggagta ccgccgccga atgggacggc tcctgcattt gcaacatacg cgggaaccat
     4381 tatccagaat gctgcaacaa acgtcgtcac aactgcaaga attgtctcca cgatcatcag
     4441 atcacctgat ccatagttta tttaatagaa agcagaaaga tataataact tacattttgg
     4501 cggtctgatc cgccaatacc caaggagata gcgggtccat gaaaatcatc aaagaaacaa
     4561 aaggcatgtg tccggtctgc ggcaaactgc tccccgcaac agttactgaa gaggatggag
     4621 ctatctggat tcgcagaacc tgcccggacc atggagagtt ccgatccctt tactggtcgg
     4681 atgcagagat gtaccaccgg tttgagcagt atgaccgtga aggccgtggt gtctataatc
     4741 cgaacaccaa tactcccggt gagtgtaccc agcgctgcgg tttatgttcc caccataaat
     4801 ccggtaccct tcttgcaaac atcgatctca caaaccggtg taatctgaac tgtgaattct
     4861 gtttcgccaa cgctcgtgcc tgcggatata tatatgagcc gacgtttgac gaaattgtct
     4921 caatgctttc actgctcaga aatgaggaac cggtgccgtg tccggcagtt cagttcgcag
     4981 gcggcgagcc aacgatgcgc gatgatctgg tagagattat caagaaagca aaggagttcg
     5041 gattttctca ggtccagatg gcatcaaacg gtatcaagct tgcacgcaat cctgatcttg
     5101 ccaaagagct gcgggaggcg ggtctttcaa cggtgtatct gcactttgac ggaaccacca
     5161 aagagacgaa tccgcttctg gaaaaaacca gtcttccggc gattgaaaac tgccggaaag
     5221 caggtcttgg aattgttctt gtaccaacag tcatcaacgg caaaaatgat caccagattg
     5281 gcgacattat ccggtttgcc gcaaagaaca ttgatgtgat tcgcggtatc aacttccagc
     5341 cggtggcgtt taccggtgct gccaagaatg atgatgtgga gcgggaacgt gtaaccgttc
     5401 cggatctggt cgaacgtatg gaggaacaga ccggcggcgc attaaggagg actgacttct
     5461 acccggtacc gtccatgatg tccgtctgtg atctgatcga gtcgtacacc ggtgaagcac
     5521 agattatgtt ctgcgcccac cctcactgcg gtgctgcaac ctatggtttt gtcaatgaaa
     5581 aaggtgacat tgtcccggtc aatcgtttca ttgatgtgga taagttcctg agtgaagttg
     5641 ccaagatggc agagaagttc cggagtgcag ggaagaccgg cagatacctt gctatggtta
     5701 ccggtattaa caacatgcga aaaattgtgg agaagggaga ggtcgagggt attaagatcg
     5761 acatgaacaa aatgatctac aatgccctga tcaaacacga ctacaacagt atcggtcagt
     5821 tccacaagaa tgccctgttt atcggaacca tgcactttat ggactgcttc aactacgaca
     5881 ccgatcgtgt tgagcggtgc tgtatccact atgcaacacc tgacggccgc ctgattccgt
     5941 tctgtacata caacagcggt cctgtgtatc gcgaacaggt ctggaagaag tacgctaaac
     6001 cgatgccggc caaaaagaac gcatctgata cctcctgtgc aggatgtgat gcgtgcggtt
     6061 ccgtagatac tacggaataa tcttttttca tctgttagcc tgtctggttt catcttgagg
     6121 gaaaatgatg ataccagagg atccatcccg gaaactttat tcctgcacct catgcaagta
     6181 ttacacaata tatgatcctc tcaaccggtc ctggttccac ccgtctctgc cgaaaagttg
     6241 cagacgctct ctcccttcca gaagagtcag ttgcagctgt tgcatctctg tttggca
//
LOCUS       DALTZZ010000079         2919 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_11596,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000079 DALTZZ010000000
VERSION     DALTZZ010000079.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11596"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="O0X12_01355"
     CDS             complement(<1..737)
                     /locus_tag="O0X12_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HJK52840.1"
                     /translation="MYIGIDHGTTSLRFAADNGKRFKMSREAAKEFTIEDLQKLGPVE
                     DIAGIALCYSMGDNFTQITPVDKLKNRGVITRDGAGEHIGGGTRVFDAVRESGIPAIA
                     IPGIHRGSDTDPRFKIYSHQTSPEKLGIAYLVSETLGDTFIVSDISSNTVSLLIANGK
                     VIGAFDACVFAPGTRHGALDVDAIRKVDAGEITANEAFTTAGVMYHLEEKYQNPTVAM
                     WAAMECASLSLLAPDASIALAGSLAPEL"
     gene            832..1602
                     /locus_tag="O0X12_01360"
     CDS             832..1602
                     /locus_tag="O0X12_01360"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HJK52841.1"
                     /translation="MAETRTHYTILGVPQNASPEMIKKAYVLLVKQYHPDRAGSDEAK
                     QEEYNERLLEIMASYEILSDPVKRAEYDAELSGTESASGTPRGKKMCGMPVKGGDIET
                     DYPISMAIAAYGKGKIPMKVAGERVVVKVYPGVRRYRIEGYGVPVEPGKPRGDLYVNL
                     KVIPQENWEIDDATNNLIHTLQITQKQAERGGQVSVKLLFNKVIKITVPAGVKTGDRF
                     TPPEGKGLGVMSPKKRGDLVVAVEVVEKKGFLSGLFGK"
     gene            1610..2326
                     /locus_tag="O0X12_01365"
     CDS             1610..2326
                     /locus_tag="O0X12_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52842.1"
                     /translation="MAEKQRGNLTAADFCHALYRRFDAAAADGEPDLVVSAGDLHESL
                     KAASRLSLCCNCMYDTQNIGDSIVNVPSGGVGATLSIRYALPREKGLLLEKSGYAAAA
                     VKSGAQLRTRRFEELARVHPVLRDLAVIARQKKSETATRKLCGITSAVAELVCRTQHI
                     RIDNKKFGTLSGAIEKADIFTPAGLHAMNFVRIVGNTHARKIPDAYLMTPNVFAYTGH
                     AFLIFADEVVDRRLIWKKSE"
     gene            complement(2349..>2919)
                     /locus_tag="O0X12_01370"
     CDS             complement(2349..>2919)
                     /locus_tag="O0X12_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HJK52843.1"
                     /translation="GARVIVTEIDPRRALQAHFDGFDVMSMNEAAKIGDLFITTTGNC
                     SIITGRHFPLMKSGAILSNAGHFNNEIEIPWLAEHATVVEHRDGIDTYVIDGKKIHLL
                     AEGRLVNLATPKGMGHPVEVMDLSFAVQALSVEYVAKHGRELAAGVHDVPTEIDEYIA
                     RLKLAAVGATIDELTDAQKEYMCSWNHGT"
BASE COUNT          678 a          734 c          802 g          705 t
ORIGIN      
        1 agttcgggtg caagactccc ggcaagggca atggaagcat ccggtgcaag cagggaaagc
       61 gatgcacatt ccattgctgc ccacatggcg acggtcgggt tttggtattt ttcctccaga
      121 tgatacatca cgcctgcggt ggtgaaggct tcgtttgcgg ttatttcgcc tgcatcaacc
      181 tttctgatag catcaacgtc aagggccccg tgacgagtgc cgggcgcaaa cacacaggca
      241 tcaaaggcac cgatcacttt tccgttcgcg atcagcagtg agacggtgtt tgagctgatg
      301 tcggatacga tgaaggtatc accgagtgtc tcggaaacaa ggtatgcgat accgagtttt
      361 tccgggctgg tctggtgcga gtaaatcttg aaacgcggat cggtgtcaga cccacggtga
      421 atgcccggga tggcaattgc cggaatgcct gattcccgta ccgcatcaaa tacacgtgtt
      481 cccccgccga tatgctctcc cgcaccgtcg cgggtaatga cgccgcggtt tttcagttta
      541 tcaaccggcg ttatctgcgt gaagttgtca cccatcgagt agcagagggc aatgcctgca
      601 atatcctcaa caggtccgag tttctgcaaa tcctcaattg taaactcttt tgctgcttca
      661 cgggacatct tgaaccgttt cccgttgtct gcagcaaaac ggagcgaggt tgtcccgtga
      721 tcaatcccaa tgtacatggg aattatattt ggaggaagtc ctgataatgg ttggtctcct
      781 gtattttccc agtaagatta tgattttgtc ccaccaacat gatctcattt catggccgaa
      841 accagaactc actatactat cctgggtgtt ccgcagaatg caagcccgga gatgatcaaa
      901 aaggcctacg tcttgttggt gaagcagtat catccggatc gtgcgggatc tgatgaggca
      961 aaacaggagg agtacaatga gcgtctgctt gagatcatgg caagctatga gatcctctct
     1021 gatccggtga agcgtgccga gtatgatgcg gagctgagcg gtaccgagtc cgcatccgga
     1081 accccgcgcg gaaaaaagat gtgcggtatg ccggtgaagg gcggggatat tgaaacggac
     1141 tatccgatct ccatggctat tgctgcctat ggcaaaggca agatccccat gaaggttgct
     1201 ggtgaacgcg tcgtcgtcaa ggtctatccg ggagttcgcc gctaccgcat cgaagggtac
     1261 ggcgttcctg ttgaaccggg aaaaccccgc ggtgatctgt atgtgaacct gaaagtaatt
     1321 ccgcaggaga actgggagat tgatgacgca accaacaacc tgatccatac actgcagatt
     1381 acgcaaaaac aggcggaacg cggcgggcag gtttctgtga agctgttgtt caataaagtg
     1441 attaaaatca cggttcctgc gggggtaaag accggcgatc gctttactcc tccggaagga
     1501 aaaggtcttg gcgtaatgtc gccgaagaaa cgcggggatc tggttgtcgc ggttgaggtt
     1561 gtggaaaaga aaggattcct ttccggtctc tttgggaagt gaacgctctg tggcggaaaa
     1621 acagcggggc aacctgaccg cagcagactt ttgccatgca ctctaccgca gatttgacgc
     1681 agccgctgca gacggtgaac cggatcttgt ggtgagtgca ggagatctgc acgaatccct
     1741 taaagcagca agccgccttt ctctttgctg taactgcatg tatgatacgc agaacatcgg
     1801 tgacagtatt gtgaatgtcc cttccggcgg tgtgggtgca accctgtcca ttcggtacgc
     1861 attgccgcgc gagaaagggc ttctgctgga aaagagcggg tacgccgcag ctgcggtgaa
     1921 atccggtgca cagctgcgga cccggcggtt tgaggaactt gcccgcgtgc atccggtctt
     1981 acgtgatctt gcggtgattg cccggcagaa aaagtccgag acagccacac gaaaactctg
     2041 cggcattacg tccgcggttg cggagcttgt atgccggacc cagcacatcc ggatcgacaa
     2101 caaaaagttc gggacgctct ccggtgcgat cgaaaaagct gatatcttca cacccgcggg
     2161 actgcatgcg atgaattttg tgcggattgt cggcaacacg catgcacgaa aaattcctga
     2221 tgcgtatctg atgacaccga atgtgtttgc ctataccggt catgcgtttc tgatctttgc
     2281 agatgaggtt gttgaccgtc ggctcatctg gaaaaaaagt gaataaaaaa aatatttttt
     2341 ttatttcgtt atgttccgtg gttccagctg cacatgtact ctttctgcgc atctgttaac
     2401 tcatcaatcg tcgcaccgac agctgcaagt ttcaggcggg caatgtactc atcgatctcg
     2461 gtcggcacat catgcactcc ggctgcaagt tcgcggccgt gctttgccac gtactcaaca
     2521 gacagtgcct gtaccgcaaa gctgagatcc attacctcaa ccgggtgacc catacctttc
     2581 ggggttgcaa ggttgaccag tcttccttcc gcaagcagat ggatcttctt tccgtcgatc
     2641 acgtacgtgt caataccgtc gcggtgctca actactgttg catgttctgc aagccacggg
     2701 atctcaatct cgttgttgaa gtgtcctgca ttggaaagaa ttgcacctga cttcatcagc
     2761 gggaagtgac ggccggtgat gatactgcag ttaccggttg ttgtgatgaa cagatctccg
     2821 atctttgctg cttcgttcat actcattaca tcaaagccgt caaagtgtgc ctgcagtgcc
     2881 cggcgcggat cgatttcggt gacaatcacg cgtgcaccg
//
LOCUS       DALTZZ010000080         4096 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_11844,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000080 DALTZZ010000000
VERSION     DALTZZ010000080.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11844"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="O0X12_01375"
     CDS             <1..319
                     /locus_tag="O0X12_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52844.1"
                     /translation="PLAGQEVTYGYKIEEIVEDPIAKLAGTIRLFTGREMKVSNAHKN
                     FVSVEVPPMMAMYNQNWMMTEYMITQEAFGLFPEIESVKFVETFPRPNLKVDEPAEEK
                     KEE"
     gene            612..1079
                     /locus_tag="O0X12_01380"
     CDS             612..1079
                     /locus_tag="O0X12_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HJK52845.1"
                     /translation="MKKGFAFLLAIICLIAFCMVPASAATVDAADTVDDDLVISALEI
                     PVYGPKGSATGTIVQGERKTYSFEVTPGDTEMDIQLSWESTSNNLALIFTSPSGVIYG
                     AYDDYYDSSTPNARFGIHIQSAGAVLPHGTWLFDVSGRSVSGSETFTLIINSY"
     gene            1276..1941
                     /locus_tag="O0X12_01385"
     CDS             1276..1941
                     /locus_tag="O0X12_01385"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HJK52846.1"
                     /translation="MVDEAQYNIIDSYSSLIGLPSTFITEILATIFILLGAGACTLLL
                     TRWRLPEDPNSRPRVLLNTIREHPGATQSELQKLTGHSRGSVSVNLHHLSMTSKIRKR
                     VQNGKTRYYLACTPDDEMKGFLRKLTTQENPQKILEAIISNPGISQKELHETTGIPKT
                     TLQWHLSQLAKYEAIQSTRERNTVHYNVIPDLVLLYNNALEETKQNNTSSEEKEPDNN
                     KES"
     gene            2029..2844
                     /locus_tag="O0X12_01390"
     CDS             2029..2844
                     /locus_tag="O0X12_01390"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HJK52847.1"
                     /translation="MNKQHLLIILLLLISLASTGAAADEGQYYPLDGIHITPQNEPIE
                     NNPDDTTPLVEKTGLFSIKDALHRTFITTYNQIFGFPPAIITELGSLFFLLLGGGALT
                     GLIIRRTQKPHANPRTQTIYSLIQKKPGITVAELQDNTGYSRGSINYSLTRLILADRI
                     KKIPLKKTNHYYHIEDKEDRSLEFMKHLIQQERPQKIFRTIMNTPGITQKELAETTDI
                     PTTTLQWHLTQLAKYEAITTSRRKNTISYTVLPEYIWLYHHLTKETETTSTAE"
     gene            complement(2870..3067)
                     /locus_tag="O0X12_01395"
     CDS             complement(2870..3067)
                     /locus_tag="O0X12_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52848.1"
                     /translation="MASGDPIWTAVFVVVAVMIVILFVRMVKNHLCDAGSCCGNCSRS
                     GGKSGGCSHCKGASRHSSLKK"
     gene            complement(3054..>4096)
                     /gene="feoB"
                     /locus_tag="O0X12_01400"
     CDS             complement(3054..>4096)
                     /gene="feoB"
                     /locus_tag="O0X12_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HJK52849.1"
                     /translation="VFVGEVLGPSMPDWAVSFICDGVIAGVGSVVVFLPNILLMFLLL
                     AILEDSGYLARVAVIMDQIMHKIGLHGKSFIPMILGFGCSVPAVMGARTLETERERKL
                     TVLMTPYMACSARLPVFVLLVGAFFAPQMQGAVMFSIYLLGIIVALIVGLIMQKTVMK
                     GESSSFVIEMPPYRIPTIKGVILHAVEHGGLFLRKAGLIIFPAVLLIWLLATLPYGVE
                     YGSSESIIGMIGEAIAPIFAPLGWGIPEAAVAVIMGLLAKETVVASLGTLFGVGEEML
                     GETLVNVFTPLSAYSLMVFVLLYMPCLAAMLTIKQETSWRFATGAAVLMCVVAWVVSC
                     IVYQGGVLLGFG"
BASE COUNT         1290 a         1157 c          830 g          819 t
ORIGIN      
        1 cccgcttgca ggtcaggagg tcacctatgg gtacaagatt gaggagatcg ttgaggatcc
       61 gatcgcaaag cttgcaggaa cgatccgtct cttcaccgga cgcgagatga aggtcagcaa
      121 tgcacacaag aactttgtct ccgttgaggt tccgccgatg atggcgatgt acaatcagaa
      181 ctggatgatg accgagtaca tgatcacgca ggaggctttt ggtctcttcc cggagattga
      241 gtcggtcaag ttcgtggaga ccttcccccg cccgaatctc aaggtcgatg agccggccga
      301 agagaagaag gaagaataat accgatttag ccgccggtca tccggcacat ttttttcatt
      361 tccccgttga tttcatccaa agctgacctt ttttgataat atataaatct gttgagcgcg
      421 aattaggttt tgtcagacaa aaacgtatag atcgaagatc tagaggcagg ggatgcgaat
      481 tcctccggca aaccggaaat ggatagaata tgtgcggagg ttttggatcg taaattgtat
      541 gatttcggaa tacctaaacc taatttcagc tcatacagat tataaccggt aaacgagaac
      601 aaacgtattg tatgaagaaa ggatttgctt tcctgcttgc gatcatctgt ctgatcgcat
      661 tttgtatggt acctgcttcc gcagcaacgg ttgatgcggc agatactgtc gatgatgacc
      721 tcgtaatttc agcactggag attccggtgt atggaccaaa aggttctgct accggaacga
      781 tagtacaggg agagaggaaa acgtactcct ttgaagtgac accaggagat actgagatgg
      841 atatccagct ttcatgggaa tcaacatcaa acaatcttgc ccttatattt accagtccaa
      901 gtggagtaat ctacggtgcg tatgatgatt actatgactc ttctaccccc aatgcaagat
      961 ttggaattca tatacagtca gcaggtgcag tgctgccaca tggaacgtgg ttatttgatg
     1021 tgagtggaag aagtgtgagc ggatcggaaa catttacgct gattataaac tcctattaat
     1081 ggaatgtata tgctaaaaag aacactcctt atctcttttt tgcttcttct gtgtatttca
     1141 ggaataagta ctgccgatac accagatgga gcgacatact acccaaaaga taacctgttt
     1201 atatcccaag gattagatga aattccaagt gatgataccc aaccagttgt cagaaggcag
     1261 acagacatca gatcattggt agatgaagcg caatataaca ttatagattc atacagttct
     1321 cttataggcc ttccctccac attcatcaca gaaatcctcg ccaccatctt catcctcctt
     1381 ggtgcaggcg catgcacact tcttctcacc cgctggagac ttcccgaaga tccgaactct
     1441 cgtccaaggg ttctgctcaa caccatccgg gaacatcctg gtgcaaccca atcagaactc
     1501 cagaaactta ccggtcactc acgcggctcc gtctccgtca atctgcatca cctgagcatg
     1561 acatcaaaga tccggaaaag agtacaaaac ggcaagaccc gctactacct tgcatgcact
     1621 cctgatgacg agatgaaagg atttctgcgc aaactcacca cccaggaaaa cccgcagaag
     1681 atcctggaag caatcatcag caaccccgga atctcccaga aagaactgca cgaaacaaca
     1741 ggaatcccca agacaacact ccagtggcat ctctcacagc ttgcaaagta cgaggcaatc
     1801 caaagtacac gggaacgaaa caccgtccac tacaacgtca tcccggacct tgtactcctc
     1861 tacaacaacg ctctcgaaga aacaaaacag aacaacacat catcagagga aaaagaacca
     1921 gacaacaaca aagaatcata accacatctc ttttttccag aactcaccat tcacagaaac
     1981 tctcatctac gcaaacgagc aacacaatag tactcgggaa agccactcat gaacaaacaa
     2041 catctcctca tcatccttct cctcctcata tccctcgcat ccaccggagc cgcagcggat
     2101 gaaggccagt actatcccct ggacggaata cacatcaccc cgcagaatga acccatcgaa
     2161 aacaaccctg acgacaccac acccctcgtg gaaaaaacag gactgttctc gatcaaagac
     2221 gctctgcacc ggaccttcat caccacatac aaccagatct tcggctttcc tcctgcaatc
     2281 atcactgaac ttggatcact gttcttcctc ctcctcggcg ggggagcact caccggcctt
     2341 atcatccgca gaacacaaaa accgcacgca aacccgcgta cacagaccat ctacagcctc
     2401 atccaaaaaa agcccggcat caccgtcgca gaacttcagg ataacaccgg atactcccgc
     2461 ggatccatca actacagtct taccagactc atccttgcag acaggatcaa aaaaattccg
     2521 ctcaaaaaaa caaaccacta ctaccacatc gaggacaaag aggacagatc acttgagttc
     2581 atgaaacacc tcattcagca ggaaagaccg caaaagatct tccggacaat catgaacacc
     2641 cccggcatca cgcagaaaga acttgccgaa acaacagaca tcccgacaac aacccttcag
     2701 tggcacctta cccagcttgc aaaatacgaa gcaatcacaa ccagccgcag aaaaaatacc
     2761 atatcctaca cagtacttcc cgaatacatc tggctctatc accatctcac caaagaaacg
     2821 gaaaccacaa gtactgccga ataacacaaa acggaatcaa aaaagaaact tactttttca
     2881 aagaagagtg gcgggatgcc cccttacagt ggctgcatcc cccactcttc cctccagatc
     2941 tggaacaatt gccgcagcag gaacctgcat cacacagatg atttttcacc atccgcacaa
     3001 acaggatgac aatcatcacg gcaaccacga caaacactgc cgtccagatc ggatcacccg
     3061 aagccaagaa gaacccctcc ctgatacaca atacaggaca ccacccaggc gacaacacac
     3121 atcagaacag ctgctcctgt ggcaaacctc caggaagtct cctgcttgat cgtaagcatt
     3181 gcggcaagac acggcatgta gaggagaaca aacaccatca gagagtatgc cgacagcggt
     3241 gtaaacacat tcaccagcgt ctcgccaagc atctcctcgc caacgccgaa gagtgtacca
     3301 agggatgcga ccaccgtctc ctttgcaagc agacccatga taaccgcaac cgcggcttcc
     3361 gggatacccc agccaagcgg tgcaaagatc ggcgcaatag cctcaccaat cataccaatg
     3421 atcgactcag aggaaccata ctcaacaccg tacggaagag ttgcaagcag ccagatcagc
     3481 agaaccgccg ggaagatgat aagtccagcc tttctcagga acagaccgcc gtgttcgacc
     3541 gcatggagaa taacaccctt aatggtcggg atacggtagg gcggcatctc aataacaaac
     3601 gatgacgact cacccttcat aaccgtcttc tgcataatga gaccaacaat cagcgcaaca
     3661 atgatgccga gcagatagat cgaaaacata accgcaccct gcatctgcgg agcaaagaat
     3721 gcaccaacca gcaggacaaa caccggcagc cgtgcagaac aggccatata cggcgtcata
     3781 agaacggtaa gcttacgctc acgctcggtc tcaagagttc ttgcacccat aacggccgga
     3841 acactgcacc cgaatccgag aatcatcgga atgaacgact ttccatgcag accgattttg
     3901 tgcatgatct gatccataat aactgccaca cgtgcaagat accctgaatc ctccagaatt
     3961 gccagaagca ggaacatcag cagaatgttt ggcaggaaca caacaacgga accgacaccg
     4021 gcaataactc catcacagat aaacgaaact gcccagtcag gcatgcttgg accaagaacc
     4081 tctccgacaa acacag
//
LOCUS       DALTZZ010000081         2121 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_11849,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000081 DALTZZ010000000
VERSION     DALTZZ010000081.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11849"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="O0X12_01405"
     CDS             complement(<1..618)
                     /locus_tag="O0X12_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HJK52850.1"
                     /translation="MREVAVIGAGITAFGERWDTSFRDLCTEAGVAALEDANVAGEQI
                     DAMFVGSMSAGRFIGQEHVGALVADYVGLGGELHVPATRVEAACASGGLAFRQAVAAV
                     ASGMSDVVVAAGVEKMTDVSDATDVLAGAADREWESFAGATFPGLYAMIACDYMNKYG
                     LTREQLAQVAVKNHYHGARNPYAQFRSEITQSTVIKSTLVASPLRL"
     gene            complement(628..1671)
                     /locus_tag="O0X12_01410"
     CDS             complement(628..1671)
                     /locus_tag="O0X12_01410"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HJK52851.1"
                     /translation="MVGIITYGAYIPRYRIKVEEIARVWGANAAEIKGGLGVFEKAVP
                     DYDENTATIAVEAARSALARRAVDPAEIKAVYVGSESHPYAVKPTAVTVGEAIGATPV
                     MTAADYEFACKAGTAGIQTAMGLVSSGMMKYAVAIGADTAQGAPGDALEYTAAAGGAA
                     YVIGNDDPIAVINHTCSFSTDTPDFWRREGEDYPRHGGRFSGEPGYFKHVTGAARMML
                     EHMGTKPADYDYAVFHQPNAKFPQKGAAMLGFTHDQIKTGLLAPRLGNTYSGAVPLGL
                     AAVLDIAKPGDRIFITSYGSGSGSDAFDLTVTDAILDKIDRTKGPSVEAMLAAGKYLD
                     YAVYARHKGKIKV"
     gene            complement(1665..>2121)
                     /locus_tag="O0X12_01415"
     CDS             complement(1665..>2121)
                     /locus_tag="O0X12_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HJK52852.1"
                     /translation="YIQKYGKLLFSDYDDEVIYDIHDFASSVPIRELSELIECTLLYG
                     SEEHQIFGYTVVNSQNAILNLSPNEFNRIYGWSTERALIFTDVSTGKSPMIAIRVTPF
                     KPPYVILQGIDMDNVHPLVKQLAEKDRITVLCTSLDVQEIITRLRNTEW"
BASE COUNT          488 a          571 c          515 g          547 t
ORIGIN      
        1 aagccgcagc ggtgatgcaa caagcgtgga ttttatgact gttgactgtg tgatctctga
       61 tctgaactgt gcatacggat tacgtgcgcc gtggtagtgg ttctttaccg caacctgtgc
      121 cagctgttca cgggtaaggc cgtacttgtt catgtagtca caggcgatca ttgcataaag
      181 acccgggaat gttgctcctg caaaactctc ccactcacgg tcggctgctc cggcgaggac
      241 atcggttgca tccgaaacat ccgtcatctt ctcaacacct gcggcgacaa ccacatcgga
      301 catgccggac gctactgctg caactgcctg gcggaatgca agacctccgg aagcacatgc
      361 tgcttccaca cgggttgccg gtacatggag ttctccaccg agacctacgt aatcggcaac
      421 aagagcacct acatgttcct ggccaataaa tcttccagca gacatcgatc cgacaaacat
      481 tgcatcgatc tgttcgcctg caacatttgc atcctcaagg gctgcaacac ctgcttccgt
      541 acacagatct cggaacgagg tgtcccaacg ctctccaaat gccgtaattc cggcaccaat
      601 aactgctact tctctcattt ttgcacctca caccttgatc tttcctttgt ggcgtgcgta
      661 caccgcataa tcaagatact ttcctgcggc aagcattgcc tcaacgctcg gtcctttggt
      721 tctgtcgatc ttgtcaagaa tcgcatccgt tacagtcaga tcaaatgcat ctgatcctga
      781 accgctgccg taacttgtta tgaaaatacg atctccgggt tttgcaatat caagtactgc
      841 tgcaaggcca agcggtactg caccactgta ggtgtttcca agtcttggag caagaagtcc
      901 ggtcttgatc tgatcatgtg tgaaaccaag catcgcagca cctttctgag ggaactttgc
      961 gtttggctgg tggaacacag cataatcgta gtccgcgggc tttgtaccca tgtgttccaa
     1021 catcatgcgt gcagccccgg ttacgtgctt gaagtatcca ggttcaccgg agaaccgtcc
     1081 accgtggcgg gggtaatcct caccttcacg gcgccagaag tcaggcgtat cggttgaaaa
     1141 tgaacaggtg tggttaatca ctgcaattgg atcgtcgttt ccaatcacgt atgctgcgcc
     1201 gccggcagct gcagtgtact ccagagcatc gccgggagcg ccctgtgccg tatccgcacc
     1261 gatcgcaacc gcatacttca tcataccgga agataccaag cccattgctg tctgaattcc
     1321 ggcagttcct gctttacagg caaactcgta gtcggctgcg gtcataaccg gtgttgcacc
     1381 gatggcttca cctaccgtta ctgcggttgg tttgactgca taggggtgtg actcggaacc
     1441 tacatagact gctttaatct ctgcgggatc aactgctctg cgtgcaaggg cgctgcgggc
     1501 tgcttccacc gcaattgttg ctgtgttttc atcatagtcg ggaacggctt tttcgaaaac
     1561 accaaggcca cccttaattt ctgcggcatt tgcaccccag acacgtgcga tctcctcgac
     1621 cttaattctg taacggggga tgtatgcccc ataggtaata attcctacca ttctgtgttc
     1681 ctcaggcggg taataatttc ctgcacatca agcgatgtac ataatacggt tatgcggtcc
     1741 ttctctgcaa gctgttttac cagcgggtga acattgtcca tatcaattcc ctgtaagatg
     1801 acatacgggg gtttgaacgg cgttacgcga atggcaatca ttggcgactt tccggtggaa
     1861 acgtccgtaa agatcagtgc tcgttcggtg ctccatccgt aaatgcggtt gaactcattt
     1921 ggagaaaggt tcagaattgc attttggctg ttcaccacgg tatacccaaa gatctgatgt
     1981 tcttcggatc cgtaaagaag ggtgcattcg atcagttccg acaactctcg aatcggaact
     2041 gatgaggcga aatcatggat gtcatagatg acttcatcat cgtagtcaga gaagagaagc
     2101 ttgccgtact tctgaatgta c
//
LOCUS       DALTZZ010000082         1757 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_12168,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000082 DALTZZ010000000
VERSION     DALTZZ010000082.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12168"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="O0X12_01420"
     CDS             complement(<1..1217)
                     /locus_tag="O0X12_01420"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HJK52853.1"
                     /translation="MTTKMDEGYRLYGATVLAQLAADMAGESAGVKAGTDIEYIHRMR
                     VASRRLRAALPLFAGCFPKKEYKHWENEIKQITRSLGRARDLDVQIAFLREYAEKQAC
                     AALPPGTPRFLPEAFRIQEKQEDTAPALPPAEPEPQPKEKPGLFLRIKQFFAGTPEVP
                     AEPEPIRPREKKTPGTDQLSGIECLTLRLMQERTALQPDVVRAVERFEQSKTLPSLQK
                     ACMEIIVRGRLDRTETTTTASYNAAYYAISLAAEQVKLYAKALADPERVTAHHEMRIA
                     AKKLRYTMETYRGLYGDGLAPAIRMVKDLQDYLGDMHDCDVWIEFLPRFLEKEEETCT
                     AYFGNTDFMAVLRPGIAALEENRKAKRAKLHAEANDFWKKNETQWQALEQNLTKPLLD
                     LRLKTLTIPDSVSA"
     gene            complement(1207..>1757)
                     /locus_tag="O0X12_01425"
     CDS             complement(1207..>1757)
                     /locus_tag="O0X12_01425"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285671.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HJK52854.1"
                     /translation="GTMRKEIIARIDREIERQKTHGDGYLAFKLNALVDEACIVALYR
                     ASQAGVKIDLVVRGVCCLRPEVPGVSDNIRVISIVGRFLEHTRIYYFRNGGDEEVLLG
                     SADLMPRNLSRRVEILFPVENPHLRDMIINTILKTHIKDTAQAHVLHRDGSYEKVLPA
                     EGEAPLNSQMWMMEHRGIWHDY"
BASE COUNT          283 a          457 c          582 g          435 t
ORIGIN      
        1 gccgagaccg agtcggggat ggtgagggtc ttgagccgga ggtcaagcag cggttttgtc
       61 aggttctgtt ccagggcctg ccactgggtt tcgttctttt tccaaaaatc gtttgcctct
      121 gcatgcagtt ttgcacgttt ggccttccgg ttctcttcga gtgctgcaat gcccggtctg
      181 agcaccgcca tgaagtcggt gttgccgaag taggcggtgc aggtttcctc ctccttttca
      241 aggaaccggg gcaggaactc tatccagacg tcgcagtcgt gcatgtcgcc gagatagtcc
      301 tgaagatcct ttaccattct gattgcgggc gcaaggccgt cgccgtacag accgcggtag
      361 gtctccatgg tgtagcggag tttctttgcg gctatccgca tctcatggtg cgcagtgacg
      421 cgttcggggt cggcgagggc ttttgcatac agtttcacct gttctgcggc gaggctgatc
      481 gcgtagtatg cggcgttgta ggaggcggtg gtggtggtct cggtccggtc gagtctgccg
      541 cggacgatga tctccatgca ggctttctgg agggagggaa gggttttgga ctgctcaaac
      601 cgttcaactg cacggacgac gtccggctgg agtgcggttc tctcctgcat cagccgcagg
      661 gtgagacact caattccgga gagctggtcg gtgcccggcg ttttcttttc gcggggacgg
      721 ataggttccg gttctgcggg gacttccggg gttccggcaa aaaattgttt tatgcgaagg
      781 aatagtcccg gtttttcctt tggctgcggt tccggttctg cgggcggcag ggcgggtgct
      841 gtgtcctctt gtttttcctg aatgcgaaag gcttccggaa ggaaccgggg ggttccggga
      901 ggaagagcgg cgcaggcctg tttttctgcg tactcccgca ggaatgcgat ctggacgtca
      961 aggtcacggg cacggccgag ggaacgggtg atctgtttga tctcgttctc ccagtgtttg
     1021 tactcctttt tcgggaagca tccggcaaaa agagggaggg ctgcccggag tctgcgggag
     1081 gcaacccgca tgcggtggat gtactcaatg tcggttcccg ctttgactcc tgcactctca
     1141 cctgccatgt cggcggcgag ctgtgcgagg acggtcgccc cgtacagccg gtatccctca
     1201 tccattttag tagtcatgcc agattccgcg gtgttccatc atccacatct gggagttgag
     1261 cggcgcttcg ccttctgcgg gcagcacctt ttcataactg ccgtcgcggt gcagcacgtg
     1321 cgcctgggcg gtgtctttga tgtgggtttt gaggatggtg ttgatgatca tgtcccgcag
     1381 atgcgggttt tcgaccggga agaggatctc gacgcggcgt gagaggtttc tcggcataag
     1441 gtcggcactt cccagaagca cctcttcgtc gccgccgttg cggaagtagt agatgcgggt
     1501 gtgttcaagg aaccggccca caatggagat gacgcggatg ttgtcggaga cgccgggaac
     1561 ctcggggcgg agacagcaga cgccgcggac gacgagatcg atcttgacgc ctgcctggct
     1621 tgcccggtag agggcaacaa tacaggcctc gtccacaagg gcgttgagtt tgaaggcgag
     1681 gtatccgtcg ccgtgggtct tctgccgctc gatctcgcgg tcgattcttg cgatgatttc
     1741 ctttctcatg gttccca
//
LOCUS       DALTZZ010000083         4757 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_12372,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000083 DALTZZ010000000
VERSION     DALTZZ010000083.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12372"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="O0X12_01430"
     CDS             <1..1196
                     /locus_tag="O0X12_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HJK52855.1"
                     /translation="IKGAYPQTQVIARAVDLFSADQLVDHGADVVLYPQQVVAKSAVN
                     HMNNLVASRNAQKLFSLLSSWSGTLGIITHKNPDPDAISSAMALSAIAANASGGKLAT
                     RILYEGNIGHQENRAFVNLLEIKMERLTPEILGECNYLALVDCVAPGMNNDLPLDTPI
                     NIVIDHHSTEGVVRLRKPEYLDSRPNIGATASIMTQYLQELYLPIDKKVATALFYGIR
                     ADTREFQRNLSPQDLHNAAYLLPLSDRSLLEVIMAPSVSQETLDVLGHAIVNREVRHG
                     YLFSNVGYVRNRDAIPQAADMLLNLEGVSTAMVYGITDTSITLSARNKDIRLHVGDVM
                     KEAFSPIPGASAGGHATMAALSIPLNAFSLVKNKDELLNMIIDPVLSNFMRIVGISEA
                     EGDES"
     gene            1186..1815
                     /locus_tag="O0X12_01435"
     CDS             1186..1815
                     /locus_tag="O0X12_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HJK52856.1"
                     /translation="MKAEEWEPYYSEILDFFGFSREDDEHAAHVLAELLPRDDIAVLR
                     ETISGQDCIVCGNAPSLADEIRKTDFSGKVIIAADAAARVLFHAGHRPDVILSDIDGM
                     DDDFLVMNDLGTILVLHAHGDNIPLVRSWVPKVRGPIVGTTQATPLAHVYNFGGFSDG
                     DRCVFCAYELGAARVSLIGFDLDDRSVDPVKHGKLMIARKLLKLCGHDI"
     gene            1805..3463
                     /locus_tag="O0X12_01440"
     CDS             1805..3463
                     /locus_tag="O0X12_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJK52857.1"
                     /translation="MTSDAVLIDGYVDEPACLGVPPYISPYIRTVAGVFAEHGITTDY
                     VTIDQLRRDPLRVATLSSYKYVVMIAGVTVPGKYLSGTPATLTELQQIGFTLRGRSVS
                     LLGGPIGFGYSPQGGAKAVAQAVSGWTAMLHGDPAAALDAYLSGGEPDGVCRYQDFDR
                     WAVLGAEIIRKHPFYPFVMCELETAKGCSRAVSGGCSFCTEPFYGLPKQRDIGAIAEE
                     VRALAAAGAAHFRLGRQPDLLAFGSFGGGEFPTPNPDALKSLFEEIRSAAPSLRTLHI
                     DNVNPGTIARHEEASREALAEIVAGHTAGDIAAFGMESADPAVVAANNLKGDADAVFR
                     AVEIVNEVGAKRRDGIPELLPGLNFIAGLAGETAETFAMNEAFLQRILSSGLMVRRVN
                     IRQLMPFEGTRAYEDNTLGQHDRLFHQFKERVRETFDVPMLTRVFPVGTVLRDVLIEV
                     SGTVSFGRQMGTYPILCGIPMQVPVGTVMDVAVVSYGQRSVTALPVPIEINRLNAAAL
                     KWLPGVSKKAAAQIAAKKPYADAAAFRSAVSSDAVAPLIELMRF"
     gene            complement(3460..3912)
                     /locus_tag="O0X12_01445"
     CDS             complement(3460..3912)
                     /locus_tag="O0X12_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJK52858.1"
                     /translation="MESIRVRPYSSADYPSICELDAPLFAGMGGPVLFRHIEELFPSL
                     FFVAENSEDEIIGYILGGIHLDDPNTGKLIRIGVVPNYQRKECGTKLTEALFSAMKER
                     NVTAIHLTVAETNKPAIAFYQKIGFMQKERIQNYFYPTTPRLVLWKTL"
     gene            4019..4288
                     /locus_tag="O0X12_01450"
     CDS             4019..4288
                     /locus_tag="O0X12_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HJK52859.1"
                     /translation="MKWQISDLFGMSVYSDKAVFLGKVEDVVLDVTNKKISGLALTSV
                     NQNVLDIKNYQGVVIPYRIVKEVGDIILVRHIPGAFKVSEPLFGE"
     gene            4304..>4757
                     /locus_tag="O0X12_01455"
     CDS             4304..>4757
                     /locus_tag="O0X12_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5'-guanylyltransferase"
                     /protein_id="HJK52860.1"
                     /translation="MKDREIFSGLITTVPFVLRLDGRSFHHLSRSNGYAKPYDPVFSR
                     CMVQTAQAMLENSGLAPSFAYTFSDEISLYVTSPVFDCRVEKLVSVAAGFASSAFTLF
                     SRSDVPLSFDCRVVPVDEGHLAEYLAWRQAEAWRNHINGYAHKLLMDTG"
BASE COUNT         1070 a         1140 c         1253 g         1294 t
ORIGIN      
        1 acattaaggg cgcttatccg cagacccagg tgattgcccg tgctgtggat ctgttctcgg
       61 cagatcagct ggtggatcac ggtgcggatg ttgtgctgta tccgcagcag gttgttgcta
      121 agtctgccgt caatcacatg aataatctgg ttgcgtcaag aaacgcccag aaacttttct
      181 cccttttaag ctcgtggagc gggacccttg gcattatcac gcacaaaaat cctgatcccg
      241 atgcaatctc cagcgcaatg gcactttccg caattgctgc aaatgcttcc ggcggaaagc
      301 ttgcaacacg catcctctat gaaggaaaca tcggtcatca ggaaaaccgt gcgttcgtta
      361 atcttcttga aatcaagatg gaaagactca caccggagat tctcggtgaa tgcaactatc
      421 ttgccctggt ggactgtgtt gctcccggca tgaacaatga cctcccgctg gatacgccga
      481 tcaatatcgt catcgatcac cacagcaccg aaggtgtagt gcgtctgcgg aaaccggagt
      541 accttgattc ccgccctaac attggcgcaa ctgcatccat tatgacgcag tatctccagg
      601 agttgtatct gccgatcgat aaaaaggttg caacagccct gttctatggt attcgtgcag
      661 atacccggga gttccagcgg aatctttctc cgcaggatct gcacaacgct gcttatcttc
      721 ttcctctgtc ggatcgatct cttcttgagg ttatcatggc accatctgtc tcacaggaaa
      781 cgctggatgt tctcggtcat gcaatcgtta accgtgaggt cagacacggg tatcttttct
      841 ccaatgttgg atacgtccgc aatcgtgatg caattccgca ggcagctgat atgctcctga
      901 atcttgaagg tgtgagtact gcaatggtgt atggtatcac tgacacctct atcacgcttt
      961 cagcccgcaa caaggacatc cgtctgcatg tcggtgatgt gatgaaagaa gcgttctctc
     1021 caattcctgg ggcatctgca ggaggtcatg caacaatggc agcactgtct attccgttga
     1081 acgcgttctc tttagtgaag aacaaagacg aactgctcaa tatgatcatt gatccggttc
     1141 tgtccaactt catgcggatt gtcggtatct ccgaggctga aggggatgaa agctgaggag
     1201 tgggaaccct actactctga aatcctcgac ttttttggat tttcacgtga ggatgatgag
     1261 catgcagcac atgttcttgc agagcttctg ccccgcgatg atattgcagt tctgcgtgag
     1321 acaatttctg gtcaggactg tatcgtttgc ggaaacgcac cgtctcttgc tgatgagatt
     1381 cgtaagactg acttttccgg taaggtcatt attgctgccg atgctgcggc gcgtgttctt
     1441 ttccatgccg gtcatcgtcc ggatgtaatt ctctctgaca tcgacgggat ggatgacgac
     1501 tttcttgtta tgaatgatct tgggacgatt ctggtactcc atgcgcacgg tgacaatatt
     1561 ccgcttgtac ggtcatgggt cccaaaggtc cgagggccga ttgtcgggac aacgcaggca
     1621 acgccgcttg cacatgtgta caacttcggc ggattttccg acggggatcg gtgtgtgttt
     1681 tgtgcctatg agcttggtgc tgctcgagtc tctctgattg ggtttgatct ggatgatcgc
     1741 tcggtggatc cggtaaagca cggaaaactg atgatcgcac gaaaacttct gaaactttgc
     1801 ggtcatgaca tctgatgcag ttctcattga cgggtatgtg gatgagcccg cctgtcttgg
     1861 tgttccgccg tacatctctc cgtacatcag aactgttgct ggtgtgtttg ctgaacacgg
     1921 cattaccact gattatgtaa cgattgatca actgcggcgt gatcctctga gggttgcaac
     1981 cctctcctcc tacaagtacg tggttatgat cgctggcgtc accgttcccg gcaaatatct
     2041 ttccgggaca cctgcaacac taaccgagct gcagcagata ggtttcacgc tgcggggaag
     2101 atcagtatca ctgcttgggg gacctattgg gtttggttac tctccacagg gtggagcaaa
     2161 agctgttgcg caggctgtct ctggatggac tgcaatgctg cacggggatc ctgcagccgc
     2221 acttgatgca tatctgtccg gtggagaacc ggatggtgtc tgccggtatc aggactttga
     2281 ccgatgggca gtgcttggag cagagattat ccgtaaacat ccgttctatc cgtttgtgat
     2341 gtgtgagctt gagaccgcaa agggatgttc ccgtgcggtt tcagggggat gttccttttg
     2401 taccgaaccg ttttacggac taccgaaaca gcgcgacatc ggtgcaattg ccgaggaggt
     2461 cagagcactt gctgcggcag gtgctgcaca tttccggctt ggtcgtcagc cggatctcct
     2521 ggcgttcgga tcatttggcg gcggtgagtt tccaaccccc aatccagacg cactgaaaag
     2581 tttgtttgag gagatccgat ctgctgcacc gtcacttcgg acgctccaca tcgacaatgt
     2641 caatccggga accattgccc ggcatgagga agcatcccgc gaagcactcg cggaaattgt
     2701 tgcagggcac acggcaggag atattgcagc gtttgggatg gagtctgccg atcccgcggt
     2761 ggttgcggca aataatctga aaggtgatgc ggatgctgtt tttcgggcag ttgagatcgt
     2821 taatgaggtt ggtgcaaagc gcagggacgg aattcctgaa cttctgcccg gtcttaactt
     2881 tattgcgggt cttgccggtg aaactgcaga gacgtttgcc atgaatgagg catttttaca
     2941 gcggatcctt tcttccggac ttatggttcg ccgtgtgaac atccgccagc tgatgccttt
     3001 tgaaggaaca cgcgcatatg aggataacac gcttggacaa cacgacagac tgttccacca
     3061 gtttaaagag cgtgttcggg aaacgtttga tgtgccgatg cttacacgtg tgttcccagt
     3121 tggtaccgtt ttgcgtgatg ttctcattga ggtgtcgggc actgtgtcat ttggcagaca
     3181 gatggggacg tacccgattc tctgcggtat tccaatgcag gttccggtag gtactgtgat
     3241 ggatgtcgcg gttgtttcct atggtcagcg gtcggtgact gcacttccgg tcccaattga
     3301 gatcaaccgg ctgaatgctg ctgcgctgaa atggctgccg ggcgtatcca aaaaagctgc
     3361 ggcacagatt gctgcaaaaa aaccgtatgc ggatgcagct gcatttcgct cagcggtttc
     3421 ctcagatgct gttgcccctc ttattgagct gatgcggttt taaagcgtct tccagagcac
     3481 aagccgagga gtagtcggat aaaagtagtt ttgaatgcgt tccttttgca tgaatcctat
     3541 tttttggtaa aacgcaattg ccggtttgtt tgtctcagcc accgtcagat ggatcgcagt
     3601 aacatttcgc tctttcattg cagaaaacag tgcctcggta agttttgtac cgcactcttt
     3661 gcgctgatag tttggaacaa ccccaatcct gatcagtttt ccggtattcg gatcatcgag
     3721 atgaatgcca ccaagaatat agccgatgat ctcatcttca gaattttctg caacaaagaa
     3781 gagagagggg aacagttcct caatgtggcg gaacagtacc ggtcccccca tacctgcaaa
     3841 cagcggcgca tccagttcgc agatggatgg ataatctgca gaggaataag ggcggacacg
     3901 gatcgattcc atatattcac aagtatatgt ggtttggaga tgttgaacat atctcatagc
     3961 ttgtcccgcg acatgcaaac atacttatgc ttgcgggagt ataattttca aaagtatgat
     4021 gaagtggcag atctcggatc tctttggcat gagcgtctat tcagacaaag ctgttttcct
     4081 tggcaaggtt gaagatgttg tcttggatgt tactaacaaa aaaatcagtg gtcttgcact
     4141 gaccagtgta aaccagaatg tacttgatat caagaactat cagggtgttg tcatacctta
     4201 tcgtatcgta aaagaggtag gagacataat tcttgtccgt catattcccg gagccttcaa
     4261 agtatccgag ccgctctttg gggaataatc tgtaaataga ataatgaagg atcgcgagat
     4321 tttttccggc cttatcacca ccgttccttt tgttttgcgg ctggacggaa gatccttcca
     4381 ccacttatcc cgcagtaacg gatatgccaa accgtatgat ccggtgtttt ccagatgtat
     4441 ggtacaaacc gcgcaggcaa tgcttgagaa cagcggcctt gcaccatcct ttgcctatac
     4501 attttcggat gagatcagcc tctatgttac gtccccagtt tttgactgtc gggttgagaa
     4561 acttgtctcg gtggccgcag gttttgcttc ttcggcgttc acattgtttt ccagatcaga
     4621 tgttccgctt tcctttgact gccgcgtagt cccggttgac gaaggtcacc ttgccgagta
     4681 tcttgcctgg aggcaggctg aggcttggcg aaatcacatt aacggctatg cgcataagct
     4741 gctcatggac acgggac
//
LOCUS       DALTZZ010000084         5405 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_12474,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000084 DALTZZ010000000
VERSION     DALTZZ010000084.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12474"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(183..437)
                     /locus_tag="O0X12_01460"
     CDS             complement(183..437)
                     /locus_tag="O0X12_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52861.1"
                     /translation="MTEPKTIRERYNDTQSRILGYLKAGVAKGSRFFKAKYIAKDLGL
                     SSKEVGTNLAILSEICDELDIQRWSYSNSTTWMVTSKTIA"
     gene            complement(614..2458)
                     /locus_tag="O0X12_01465"
     CDS             complement(614..2458)
                     /locus_tag="O0X12_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HJK52862.1"
                     /translation="MGTIDMVYRLGPGCEVDDIVEGQVYLGKVQGFATFGTFVLLNDR
                     VKGLLHKSNVKTEKKERDQILVQVNQIRPNGNIDLREVQLPEGSYETQLVTKKITLSR
                     LSDLKNKIGRNVTIEADVVQIKQTSGPTIFTVCDDSGVEDAAAFTEAGVRSYPEVNLG
                     DIVRIFGEATRRNNQIQVEVSDMHVLKGTEADAVRARINKALEARAEPPEVTPLIESP
                     VLDALWPEMRKLAKIVRRAVLTQQPIILRHHADADGICAAVAVETAVLQLIRDTGGDP
                     DQDNFLFRRSPSKAPFYEIEDVTRDLDMMLKDNVRFGQKLPLILLMDNGSTEEDMPSY
                     KMTEVYQLDVVVADHHHPDETIDKYLLAHVNPYHVGGDFGVTAGMLGTEIARLINPAV
                     EARILHFPSVAGVADRSEAPELDAYLALIEGKHSRDECKDMALALDYEQYWLRFNDGR
                     EIVKDILNLNNAPDRHNRLVALLVTEANAAIEDQMNTMMPHVKDKILASGANLFLADV
                     EMFAHRFTFPPPGKSSGEVHDILCKRYENQPVVTLGLGPDFAVIRSRGVKMNIPQMVR
                     AMRDEMPGAGISGGGHLVVGSIKFVEGMRSEVIAKMIEKISEFPVDTA"
     gene            complement(2465..3679)
                     /locus_tag="O0X12_01470"
     CDS             complement(2465..3679)
                     /locus_tag="O0X12_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HJK52863.1"
                     /translation="MMERDNQPINLKDVLIEMKDISELMVDLAYSAVLFESNAIADEV
                     IELEERMNDLVYQARITSMMSVRRLEDTEPMSGLLQLAEASERISNQAADIAKNIMRH
                     VSFPAKLRKALPEAEEATHRTVVAAGSTLDCARLGDIKLQSATGIRIIMIRRMRQRIY
                     DPDKHTVLRAGDVLVGRGPEYGFPALCELAGQPAPNDDDDVATAISDLDRAAALMIEM
                     KNISELSVGLAYTALLHENMDLANEVVALEEELDEMRLKLDLWTLEAAKRTEDVASLR
                     GMLYMSSFAESISDAASSIADVVLREIDVPPIIKRIVRESDEIISWVTVHPGSPLDKK
                     SLADSHVGTVTGMVIFAIKHENRWTYRPARSVVLHAGDLLVARGRRDGEEKLYGLCGA
                     DTDEIEDFEDSE"
     gene            complement(3795..>5405)
                     /gene="ilvD"
                     /locus_tag="O0X12_01475"
     CDS             complement(3795..>5405)
                     /gene="ilvD"
                     /locus_tag="O0X12_01475"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HJK52864.1"
                     /translation="SLFYAMGYTEEELSRPLIGVVSAHSEIVPGHIHLDKIAEAVKAG
                     VRMAGGTPILIPAIGVCDGIAMGHIGMKYSLASRELIADSVETMAMAHCLDGLVLIPN
                     CDKIVPGMLMAAARLDIPAVVCSGGPMLAGVDNGEDISLSKLFEAVGARKINAITDAD
                     LQRIEMDACPGCGSCAGMYTANSMNCLAEALGIALPGNGTIPAVMAARIQLAKHAGMK
                     VMDLVQRGITARQIITPAAIRNALACDMALGCSTNSVLHLLAISREAGVPLDLATIAD
                     VSAKTPNLCHLAPAGPAHIQDLHAAGGIPAVLTELAKGGFIDTSLLTVTGKTVAENIA
                     GSVNKNPAVIRPIENPYSPTGGIAVLWGNIAKEGCVVKRSAVADEMLVHSGPARVFDS
                     EDAAIAAIYAGKIVPGDVVVIRYEGPKGGPGMREMLNPTSALAGMKLDRQVALITDGR
                     FSGASRGASIGHVCPEAAAGGEIGLLEEGDTILIDIPNGRVNALVSDEEFAVRRSRQV
                     MPAPSVTSGWLARYARGCAGANDGAVMK"
BASE COUNT         1286 a         1416 c         1368 g         1335 t
ORIGIN      
        1 ccttttcact taccataagt aaatgtttca ttttataata tatatacttc ctctttgact
       61 cctaaaatga tacagttgga acaaaggcaa aataccgcag ggatagacaa tcgcgcagag
      121 aatatattgc tgatattggt tacaatgcgt aagggagcaa aaaagaaaga ggggtgaagt
      181 atttatgcta ttgttttgct cgttaccatc caggtggtgc tgttggagta actccagcgc
      241 tggatgtcca gttcatcaca gatctcggaa aggatggcaa ggtttgttcc aacctctttg
      301 gaggaaagac caagatcttt ggcaatgtac tttgccttga agaaacggct tccttttgcc
      361 actcctgcct tcagatagcc aaggatccga gactgggtgt cgttatatct ttcacgaatt
      421 gttttaggtt cggtcataat gacttacctc ttgtaagtat aggatgtttt acattataaa
      481 gatagtgctt gacatacagg cttgataatg aattcagcgg gaacgaccgc tgttatgaaa
      541 aagaatccac ataaccaaag gttgtggatt acctcggagt ctctgcggct ccgagacagg
      601 cagttgaaca gaatcaggct gtgtcgaccg ggaactcgga gatcttttcg atcatctttg
      661 caatgacctc agaccgcatt ccctcaacaa acttgatgct accaacaacc agatgaccgc
      721 cgcctgaaat tcctgctccc ggcatctcat cgcgcatggc acgaaccatc tgggggatgt
      781 tcatcttcac accgcgggaa cggataaccg caaagtctgg accaaggcca agggttacga
      841 ccggctgatt ctcataacgc ttgcagagaa tatcatggac ctcaccggag gatttgcccg
      901 gcggcgggaa ggtgaagcgg tgggcaaaca tctcaacatc ggcaaggaag aggttggccc
      961 ctgatgccag aatcttatct ttgacatgcg gcatcatcgt gttcatctga tcctcaattg
     1021 ctgcatttgc ttcggttaca agaagcgcaa ccagccggtt gtggcggtct ggtgcattgt
     1081 tgaggtttaa gatgtctttt acaatctcac ggccgtcatt gaacctgagc cagtactgct
     1141 cgtagtccag tgcaagtgcc atatccttac actcatcccg gctgtgttta ccttcaatga
     1201 gtgccagata tgcatcaagc tcgggagcct cactgcggtc tgcaacaccg gcaaccgagg
     1261 ggaagtgaag gatccgtgct tcaacagccg gattgatcag gcgggcaatt tcagttccaa
     1321 gcattcctgc ggtgacacca aagtcaccgc caacatgata ggggttgaca tgggcaagca
     1381 gatatttgtc aatggtttcg tccggatggt ggtgatcggc gacaacgaca tcaagctgat
     1441 acacctcggt catcttgtag gagggcatat cctcttcggt tgaaccgttg tccatcagaa
     1501 gaatcaacgg caacttttgg ccaaaccgga cgttatcctt taacatcatg tcaagatcgc
     1561 gggttacatc ctcaatctca tagaatgggg ctttggaggg ggacctgcgg aaaaggaagt
     1621 tgtcctgatc cggatcgccg ccagtgtcac ggattaactg aagaactgcc gtctcaacag
     1681 caacagcggc acagataccg tcagcatccg catggtggcg gagaatgatc ggctgctggg
     1741 tcagtaccgc acggcggacg atctttgcaa gcttacgcat ttccggccag agtgcatcaa
     1801 gcaccgggct ttcgatgagc ggggtaactt caggaggttc tgcacgggct tcaagggctt
     1861 tgttgatacg tgcacggaca gcatctgcct cggttccttt gaggacatgc atgtcggaga
     1921 cctcaacctg gatctgattg tttctgcggg ttgcctctcc aaagatacgg acgatgtcac
     1981 cgagattgac ctctggataa gatctgacac cagcttcggt gaatgctgca gcatcctcaa
     2041 caccggagtc atcacaaacg gtgaaaatgg taggtccaga tgtctgctta atctgcacga
     2101 catcagcctc aatggtgacg tttctgccga tcttgttttt caggtctgaa agacgggata
     2161 gtgtgatctt tttggtgacg agttgcgttt catagcttcc ttcgggcagc tgcacttcgc
     2221 gcagatcgat gtttccgttc gggcggatct gatttacctg tacaagaatc tgatcacgct
     2281 cttttttctc ggtcttgaca ttgcttttat gcagcagtcc tttgacgcga tcgtttaaga
     2341 gaacaaacgt accgaatgtt gcaaacccct gaacttttcc aagatatacc tggccttcta
     2401 cgatgtcatc cacttcacaa ccggggccta gccggtatac catatcaatt gtacccatgt
     2461 aaatttactc cgagtcttca aaatcttcta tttcatcggt gtctgcaccg cagagaccat
     2521 acagtttctc ttctccgtca cgccggccgc gtgctaccag caggtcgcct gcgtgcagca
     2581 caacactgcg tgcaggccgg taggtccagc ggttttcatg tttgatggca aagatgacca
     2641 taccagtgac cgttcccaca tgcgagtcgg caagggattt tttatccagc ggggaacctg
     2701 gatggaccgt gacccaagag atgatctcat ccgattcacg gacaatcctt ttgataatcg
     2761 gcggaacgtc gatctcccgg agaactacat ctgcaatcga acttgccgca tcggagatgg
     2821 attcggcaaa cgaagacatg tagagcattc cgcgaaggct tgcaacatcc tccgtacgct
     2881 ttgcagcttc aagtgtccag agatcaagtt tgaggcgcat ctcatcaagt tcctcttcca
     2941 gagcaacaac ctcgttggcg agatccatgt tttcatgaag gagtgccgtg tatgcaaggc
     3001 cgacggagag ttcactgatg tttttcatct cgatcataag ggctgccgcc cgatcgaggt
     3061 cgctgatggc agtcgcgaca tcgtcatcat catttggcgc aggctgaccg gcaagttcgc
     3121 agagtgcagg gaaaccatac tcaggccccc tcccaaccag cacatcccct gcgcgaagca
     3181 cggtatgttt gtcaggatcg tagattctct gccgcatccg gcgtatcatg ataatacgaa
     3241 taccagttgc ggactggagt ttaatgtcgc caagacgcgc acagtcaagg gtactgcccg
     3301 ccgcaacaac ggtacggtgg gttgcctctt ctgcctccgg cagtgctttt ctgagctttg
     3361 ccgggaagga aacgtgccgc atgatatttt ttgcgatatc cgcagcctgg ttggagatac
     3421 gctccgaggc ttctgcaagc tgcaggagac cgctcatcgg ttcagtgtcc tccagacggc
     3481 gtacgctcat catactggtg atacgtgcct gatatacgag gtcgttcatc cgctcttcaa
     3541 gttcgatgac ttcgtctgca atggcgttgc tctcaaagag taccgcagag taagccagat
     3601 caaccatcag ttccgagatg tccttcattt caatgaggac atccttgagg ttgatgggtt
     3661 gattatcgcg ttccatcatg ccttactatt ataatggaga cgtctgacta cttaagtcat
     3721 agtttcccac agggatttcg gttctacctg cacaaatcca cagatacctg cacatatttt
     3781 tcaaaaaagg gagattattt cattacggca ccgtcatttg ccccggcaca tccccgtgca
     3841 tatctggcaa gccagccaga ggtgaccgag ggcgcaggca tgacctgccg gctcctccgt
     3901 acggcaaact cttcgtcact gacgagagca ttgactctgc cgtttggaat gtcgatgaga
     3961 atggtgtctc cttcttcaag aagaccgatc tcaccgcccg cagctgcttc aggacagaca
     4021 tgtccgatag aggccccgcg gcttgcacct gaaaagcgtc cgtcggtgat gagcgcaacc
     4081 tgcctatcca gcttcatgcc tgcaagggcc gatgttggat tgagcatctc ccgcatgccg
     4141 ggtcctcctt tgggcccttc gtagcggatg acaacaacat cacctggaac gatctttcct
     4201 gcatagattg ccgcaattgc cgcatcctcc gagtcaaaca cacgggcagg accgctgtga
     4261 accagcatct catccgcaac ggcacttcgt ttgacgacac agccttcctt tgcaatgtta
     4321 ccccagagaa cggcaattcc tccagtggga ctatagggat tttcaatcgg tctgatgaca
     4381 gccgggtttt tgttgactga tccagcaatg ttttctgcca ctgtctttcc ggtaacagtg
     4441 agaagagatg tatcaataaa tcctcccttc gcaagctcgg tgagaaccgc aggaattccg
     4501 ccggcagcat gcagatcctg gatgtgtgcc ggacctgccg gtgcaaggtg gcagaggttg
     4561 ggggttttgg cactgacatc cgcaatcgtt gcaagatcca gcggaacacc tgcctcgcgg
     4621 gagattgcca gaaggtgaag gacactgttg gtactgcagc caagcgccat atcacaggca
     4681 agggcatttc ggatcgccgc aggggttatg atctgccggg cagttatacc ccgttgaaca
     4741 aggtccatta ctttcatacc tgcgtgtttt gccaactgaa tacgggcggc catgaccgcg
     4801 gggatagtac cgttaccggg aagggcaatg ccgagagcct ctgccagaca gttcatactg
     4861 tttgcggtgt acatcccggc acatgatccg cacccgggac aggcatccat ctcgatccgt
     4921 tgcagatccg catcggtgat cgcgtttatt tttcgtgcac caaccgcttc aaacagtttt
     4981 gaaagactga tatcctcacc attgtcaaca ccggcaagca tcggtccgcc gctgcagaca
     5041 accgcaggga tatcaaggcg tgctgctgcc atgagcatgc cgggcacaat cttgtcgcag
     5101 tttggaatga gtacaagtcc gtcaagacag tgtgccattg ccatcgtctc cacactgtcg
     5161 gcgatcagtt cacggcttgc aagcgagtac ttcatgccga tgtgtcccat cgcaatacca
     5221 tcgcagacac cgattgccgg aatcaggatc ggagtaccac ccgccatgcg gacacctgct
     5281 ttaacagctt ccgcaatttt gtccagatga atgtgaccag gaacaatttc gctgtgtgca
     5341 gataccacac cgattaaagg acgggacagc tcttcttcgg tatagcccat tgcatagaac
     5401 agtga
//
LOCUS       DALTZZ010000085         2889 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_12712,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000085 DALTZZ010000000
VERSION     DALTZZ010000085.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12712"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1096
                     /locus_tag="O0X12_01480"
     CDS             <1..1096
                     /locus_tag="O0X12_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJK52865.1"
                     /translation="YKELASAGESSSAKQKAGEIAVLYKKLSTMNPASREEYALRAVK
                     WAEIAAGKTGTSPQHPKNTAQEENSPAQTDALVTTSNVRWSDIGGLETVKQLIMETVV
                     IAGLKRPESIKPWKGILLFGPPGTGKTLLAAAAAGSLDAAFYNVKASDLLSKYFGESA
                     KLISELYESARCSAPSIVFIDEFDSLTRTRDTERSEASRTVLASLLTELDGLADKKSD
                     QLLLTLAATNSPWDMDSAVLSRFPRRIYVTLPDRRACSEILQIQLKGMDVSALSFDSL
                     AEMCVENRYAGRDLAALAQQAVWTMIHDANPNLHTLAELPFRELKERCLVTRPLQMED
                     FRPAFAKIRSPLTKDTIDQYEQWNKEYGSV"
     gene            1117..2538
                     /locus_tag="O0X12_01485"
     CDS             1117..2538
                     /locus_tag="O0X12_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HJK52866.1"
                     /translation="MKNNISKCLLAAVIIVVLMAVPAAAWVGTSSERIYPEGDLMPGE
                     SVFGVISIHFLPNSDVDRIDLYTDLASAVWTGSLRASDGSPITEFPAGKNYIDGYSLS
                     GLPQAVVLTIYLDGVVPENKAGSRISIMTIEQVSNSGEVISSYYSPRVLVSGSNRNTE
                     PLTASFTVSPRESDSLTIHFQGDSDTARTWFWDFDDGTYSSSQSPTHTFPKAGTYAVS
                     LTVEDAAGQSYTTTQWIEVTGNKDAAHLTVAFVANPDERNPLKVYFTGISELADTWYW
                     NFGDGSYSYEQSPIHTYTAPGSYTVSLTGVTPDKQTQIVTHTVEVGISATPTIQTPTT
                     KATTATVTPSQTATVDQGIPKNMVPLLIGGLAGLIIFIILLYILLRPKKKNHAGEQQN
                     TRSQNAATKPVTNQVIYQYTIQGDVHSEKIGILAKDDAVVNRSAVGGKTETQATTRFC
                     PSCWKQVPADAKYCLFCGRQLRP"
     gene            complement(2570..2883)
                     /gene="rnpB"
                     /locus_tag="O0X12_01490"
     ncRNA           complement(2570..2883)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="O0X12_01490"
                     /product="RNase P RNA component"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00373"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="archaeal type; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00373"
BASE COUNT          766 a          755 c          715 g          653 t
ORIGIN      
        1 gtacaaagaa ctggcctcag caggagagag cagctctgcc aaacaaaaag caggagagat
       61 tgcagtactc tacaaaaaac tcagcacgat gaaccccgca tcccgggaag agtatgccct
      121 gcgtgccgta aaatgggcag agatagcagc gggaaaaacc ggcacatctc cgcaacaccc
      181 aaagaacact gcccaggaag aaaactcccc tgcccaaaca gacgcactcg ttaccacctc
      241 caatgtacgc tggtcagaca ttggcggtct tgaaacggta aaacagctca ttatggaaac
      301 ggttgtaatc gccgggctca aacggcctga gtcgatcaaa ccctggaaag gcatcctcct
      361 ctttggcccg cccggaaccg gaaaaacact tcttgccgca gcagcggcag gcagtcttga
      421 tgcagcattc tataatgtga aagcaagtga tctcctctcc aaatactttg gcgagtccgc
      481 aaaactcatc tcggaactgt atgaatcagc ccggtgctcg gcaccgtcca tcgtgttcat
      541 tgacgagttt gattccctga cacggacacg cgatacagaa agaagtgaag catcacggac
      601 agttcttgca tccctcctta ccgaactgga cgggcttgcc gacaaaaaaa gcgatcagct
      661 ccttctcacc cttgctgcaa cgaactcccc atgggatatg gacagtgcgg tcctgtcccg
      721 gtttccccgg cgaatctatg tgacactccc ggatcgcagg gcgtgttctg agatcctgca
      781 aattcagctg aaaggcatgg atgtctctgc cctctcattt gattcccttg ccgagatgtg
      841 tgtggagaac cggtacgccg gccgtgatct ggcggctctg gcccagcagg ctgtatggac
      901 aatgatccat gatgcaaatc caaatctgca caccctggct gaacttccgt ttagagagtt
      961 gaaggagcgc tgtcttgtga caagaccatt acaaatggag gactttagac ctgcatttgc
     1021 aaagatccga tcccctctaa caaaagatac tattgatcag tatgagcaat ggaataagga
     1081 atatggatcg gtgtaactca tgaggaggat gatactatga agaataatat ctctaaatgc
     1141 ctgctggcgg cagtaattat cgtggttctg atggcagtgc ctgctgcggc atgggtggga
     1201 acatcctctg agaggatata tccggaggga gatctcatgc ccggagaatc ggtgttcgga
     1261 gtaatttcca tacatttcct ccccaacagc gatgtggata ggatagacct ctatacggat
     1321 ctggcgtcag cggtctggac gggaagtctt agggcatcag atggatctcc gataacagaa
     1381 tttccggcgg gaaaaaatta cattgacggt tactctctca gtggtcttcc ccaagcggtt
     1441 gttttaacca tttatctgga tggggttgtt ccggaaaaca aagccgggag tcgtatatcc
     1501 ataatgacca ttgaacaggt gagcaattcg ggtgaagtta tttcgtctta ctactctcca
     1561 agagtactcg tgtctggttc aaaccggaat acagaacccc tgactgcttc ctttactgtc
     1621 tctccgaggg agtcagattc tttgacaata cacttccagg gagactccga tacagcaaga
     1681 acatggttct gggattttga tgatggaaca tattcctcat cacagtcacc aactcacaca
     1741 tttccaaagg ccggcacgta tgcggtgagt ctcacggtag aggatgcagc ggggcagagt
     1801 tacacaacaa cacaatggat tgaagtaacg gggaataaag atgctgccca tctgactgtt
     1861 gcgtttgtcg caaatccgga tgaaagaaat cccctaaaag tgtatttcac cgggatctcg
     1921 gaactggcgg atacctggta ctggaatttt ggggatggat catactcata tgagcagtca
     1981 ccgatacata cctatacagc accaggcagc tacactgtca gccttaccgg ggttactccc
     2041 gacaaacaga cccagatagt cactcacacc gtcgaggtgg gtatatctgc aacgcccaca
     2101 atacagacgc caacaaccaa ggcaacaaca gctactgtca ccccgtctca aacagcaacc
     2161 gtagatcagg gaatcccaaa gaatatggta cctctgctga ttggaggtct ggcaggtctg
     2221 ataatattca ttatcctgct gtatattctg cttcggccga aaaagaagaa ccatgccggt
     2281 gaacaacaga atacccgttc acaaaatgcg gcgacaaagc cggtgaccaa tcaggtgatc
     2341 tatcaataca cgattcaggg ggacgtacac tcggaaaaga tcgggattct tgcaaaagac
     2401 gatgcggttg tgaatcgttc agcggttggg ggaaaaacag agacacaggc gactacgcgt
     2461 ttctgcccgt catgctggaa gcaggtaccg gctgatgcaa aatactgtct gttctgcggt
     2521 agacaactca gaccctgaac ttttttttca aaacaccttt caaaaacgtg agtcggagta
     2581 gtaagcctcc tcctgttcgt tgcggcattc agctaagcgt cgtacccgga tcatggcgtt
     2641 caggcaccgc acgcagatcc tgtttcgact tgcacccgca gggattcgcc gttccatcgg
     2701 tccctctgca tcaacgctcg acgggttacg taccgtctca ttgctccgca gaggacgtgt
     2761 cgtttctgtt ccagagccgt gactctcgcc acccgtgctt gcacacggct gcgttacctg
     2821 aatggtggga ggactttcct cagcgatccc gtaatgatca ccgtcggccg cacgctccct
     2881 ctcacgcac
//
LOCUS       DALTZZ010000086         2942 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_12950,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000086 DALTZZ010000000
VERSION     DALTZZ010000086.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12950"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(77..1993)
                     /gene="pstA"
                     /locus_tag="O0X12_01495"
     CDS             complement(77..1993)
                     /gene="pstA"
                     /locus_tag="O0X12_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HJK52867.1"
                     /translation="MTEPTHTAAHTRLLREKIIKSIWLLTALISAAAVVFILGYLIYT
                     ALPAFMEVGIPGFLFNTEWNPTGSDPSYGVAALIVDTLLVTIGALIFAVPLGIASAVY
                     LSYLAPSRIRDTVKPIIELLAGIPSVVYGFFGMVVLCSFLRTTLDIPSGFSWLAASII
                     LGIMALPTIVSVSEDALFAVPKDYKEASLGLGTTYWHTISRVLLPAAASGISAAVVLG
                     MGRAIGETMAVMMVAGNAAIIPDPIWNVLSPVRTLTATLGIEMAEVATGSMHYYALFG
                     VAVILLIIALLVNIASSWIIKRTQTPRSSDSRLSAQSRKWLKYILAAVLCVLFFCFLV
                     AALGVFLAVIVAVLLAVWYFGRAKISSSLAERCSFGVIYLCTGVVILTLAIILWDIFS
                     NGIPAISWEFLTASPSDLGRSGGIYPAIIGTLQLVGGAILVALPIGIGAAIYLIEYSR
                     ENLLTRALRTGNDLLNGTPSIVFGLFGFAFFVIYLNWGICMLAGQICLALMILPTIVR
                     TTEEALKSVPNSLREASLGLGATKWQTIKKVVLPSAAPGILTGAILSIGRAAGETAPI
                     MFTAVVFTKRFVTMDVFDPVMALPFHLYVLSTSVPGATAQQYGTAVVLIVLVMLIYLA
                     AILLRRHFQQKLLR"
     gene            complement(2023..2901)
                     /locus_tag="O0X12_01500"
     CDS             complement(2023..2901)
                     /locus_tag="O0X12_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HJK52868.1"
                     /translation="MITDGEATGLAKSYLDFILSAEGQQILSDNGFVPVSGGSTTVTP
                     TSAVTSGTISVAGSTTVLPVAQAVAEVYMDEHLSADIQISGGGSGVGVTSVSSGTADI
                     GMLSRDLKDSEKEGHDFKEYVIGRDGIALIVHPTNTDTELTLSQVKDIYQGTITNWKE
                     VGGSDSEIVLVGRDSASGTREFFTEFVLNDEDAAKTMQELNSNGAVQQSVAQTPGAIG
                     YVSLEYVDDTVKALTIDGVTPSVATVLDGTYTINRPLLMITDGEPTGLAKSYLDFILS
                     AEGQQILSDNGFVPVA"
BASE COUNT          863 a          721 c          757 g          601 t
ORIGIN      
        1 agtccacgtc aaagagagcc tgtttagaac catacgaaag gttcagatga tgagtatgaa
       61 taacgcttgt cataatttat ctcaggagtt tctgttggaa gtgtctgcgg agaagaatcg
      121 ccgcaagata gatgagcatc acaagaacaa tcagcacaac agcagtacca tattgctgtg
      181 cggttgcacc cggaacactg gtcgaaagaa catacagatg gaacggaagt gccatcaccg
      241 gatcaaagac atccatcgtg acgaaccgtt tggtaaacac caccgcagta aacataatcg
      301 gagctgtctc accagcggct ctgccgatgg aaagaattgc accggttaaa atacccggag
      361 cagcggacgg cagaacaact tttttgatcg tctgccattt ggtcgcacca agaccaagcg
      421 atgcctcgcg aagcgagttt ggaacggatt taagggcttc ctccgtggtt ctgacaatcg
      481 tcggcagaat catcaaagca agacagatct gaccggcgag catacagata ccccagttaa
      541 gatagatcac aaagaaggca aacccaaaca gaccaaacac aatcgacggg gttccgttta
      601 agagatcatt tccggtccgc agtgcacggg tcagcagatt ttccctagag tactcaatca
      661 ggtaaatcgc agcaccaata ccgatcggaa gtgcaacaag aattgcaccg cccaccagct
      721 gaagcgtacc gataattgcc ggataaatcc ctcccgaacg gccaagatca ctgggactcg
      781 ccgtcaggaa ctcccagctt attgcaggaa taccattgga gaagatatcc cagagaataa
      841 ttgcaagggt caggataaca acaccggtac agagataaat gacaccaaac gaacatcttt
      901 cggcaagact gctggaaatt tttgcacgcc cgaagtacca caccgcaagc agtactgcaa
      961 cgatcaccgc aaggaaaacg ccaagggctg caacaaggaa acagaagaac aacacgcaca
     1021 aaacagccgc gaggatgtac ttcagccatt ttctgctttg agcagaaagc cgagaatctg
     1081 aagatcgggg cgtctgggta cgcttgatga tccatgaaga ggcaatgttt accagaagtg
     1141 caataatcag cagaataact gccacaccaa agagtgcata gtagtgcata ctgccggttg
     1201 caacctcagc catctcaata ccaagggttg ctgtcagagt tcggaccggt gacaggacgt
     1261 tccagatagg atccggaatg atcgcggcat ttccggcaac catcatgacg gccattgttt
     1321 cgccgattgc acgacccatt ccaagaacaa cagcagcgga gataccggac gctgcggccg
     1381 gaagcagaac acgggagatg gtatgccagt atgtggtacc aagaccaagg gaagcctcct
     1441 tatagtcctt aggaacagca aaaagtgcat cttccgacac agaaacgatc gtcggcagag
     1501 ccataatacc aagaatgatt gatgcagcaa gccaggaaaa accgctcgga atatcgaggg
     1561 tcgtccgcag gaaactgcaa agaacaacca taccaaagaa accgtacacg acagacggga
     1621 tgccggcaag gagttcgatg atgggcttga cggtgtcacg aatcctggac ggggcaagat
     1681 aggaaagata tactgcactg gcaattccca gcggcacagc aaagatcagt gctccaattg
     1741 tgacaagcag agtatcaaca attaaggcag ctacaccata agagggatct gatccggtcg
     1801 ggttccactc ggtgttgaac aaaaatcccg gaatacccac ttccataaat gcaggaagag
     1861 cagtatagat cagatatccg aggatgaaga caacggcagc agcagaaatc aatgctgtga
     1921 gaagccagat ggatttgatt attttttccc gcaacagacg ggtatgagct gcggtatgtg
     1981 tgggttctgt cattgtttcc tcaaaaaaag attgtgtgag tcttatgcga ccggaacaaa
     2041 tccgttgtca cttaagatct gctgaccttc tgcagagagg ataaagtcaa gataactctt
     2101 tgcaaggccg gtcggttcac cgtcggtgat catcagaagc gggcggttga tggtataggt
     2161 tccgtcaagg accgtagcaa ctgaaggagt aactccatcg atggtgagtg ctttaacggt
     2221 gtcgtccaca tactcaagag agacataacc gattgcacca ggggtctggg caacactctg
     2281 ctgaactgcg ccgttggagt tgagttcctg catcgtcttt gcagcatctt catcatttaa
     2341 gacaaactca gtgaagaact cccgagtacc ggaggcactg tcacgaccga caagaacaat
     2401 ctcactgtcg gagccgccaa cctctttcca gttggtgatt gtaccctgat agatgtcttt
     2461 aacctgggaa agggtcagct cagtatcggt gtttgtcggg tgaacgatca gtgcgatccc
     2521 gtcacgtccg atgacatatt ctttgaagtc gtgtccttct ttctcagaat ctttgaggtc
     2581 acgagacagc ataccgatgt ccgcggttcc ggaagagacc gaggttacac caacaccgga
     2641 gcctcctccg ctgatctgaa tatcagcaga cagatgttcg tccatgtaca cttctgccac
     2701 agcctgagcc accggaagaa cagtcgtgga tccggcaacc gagatggtac cggaggttac
     2761 ggcagaggtt ggggttacag tagtgctgcc gccgctgaca gggacaaatc cattgtcgct
     2821 taagatctgc tgaccttctg cagagaggat aaagtcaagg tagctctttg caaggccggt
     2881 cgcctcaccg tcggtgatca tcaggagcgg acggttgatg gtataggttc cgtcaaggac
     2941 cg
//
LOCUS       DALTZZ010000087         2506 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_13131,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000087 DALTZZ010000000
VERSION     DALTZZ010000087.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13131"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(140..214)
                     /locus_tag="O0X12_01505"
     tRNA            complement(140..214)
                     /locus_tag="O0X12_01505"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(177..179),aa:Arg,seq:tct)
     gene            complement(289..684)
                     /locus_tag="O0X12_01510"
     CDS             complement(289..684)
                     /locus_tag="O0X12_01510"
                     /inference="COORDINATES: protein motif:HMM:NF016229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF473 domain-containing protein"
                     /protein_id="HJK52869.1"
                     /translation="MMQMVGIAGIAPEVIGELKKGRARTVEFVNAQNVISAASVSVGD
                     PIFVSTVPHEDLTTGDAGIVATVTAVSVTMQRMSSAAPGIYYEERERLSVRLQMKFAC
                     TSHIRKVVERRNCHVLTVEVAECSCPRVR"
     gene            complement(687..1424)
                     /locus_tag="O0X12_01515"
     CDS             complement(687..1424)
                     /locus_tag="O0X12_01515"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HJK52870.1"
                     /translation="MDITDAVRVESLAPTAKEITLLTGFPGSGLVGSIALQYLVENAG
                     FTHLGNITSRFLPQISLATNGLAQAPIRLYEKDNFVAVLADVPIPPQVSYEISAGLIE
                     WLSGKSVITELVVIGGVTTGGDGERVFGVATTTDGLETIKEQSIILPALSITGISGSM
                     IIEAQLRGIPAAGFLVETNFNVDPRAAIEALKILNTRYGFGIDTQPLMEQADQVEAMM
                     HQLANDVQEQENAEQKPFSSAEQVMYG"
     gene            1526..1870
                     /locus_tag="O0X12_01520"
     CDS             1526..1870
                     /locus_tag="O0X12_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5611 family protein"
                     /protein_id="HJK52871.1"
                     /translation="MQEYEVKRGMTKDLPDRIAAGFPDIFQIEPKVEDGHYSISYGSM
                     LRLEVWAGEKNKTVFVDTETNKDVLAMPDEEADVIILDTNKRFRQYLDLVTGFTTKER
                     VKRAKKAAEKGE"
     gene            complement(2008..>2506)
                     /locus_tag="O0X12_01525"
     CDS             complement(2008..>2506)
                     /locus_tag="O0X12_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HJK52872.1"
                     /translation="FVALTPGYVPDIESYLFGNILLVTSGDLILAGVFAVVITAVVAL
                     LYQPLLAVTFDEEYATVMNLPVKMLNLLLLILIALAVVMLIQVVGIILVIALLTLPAA
                     TCRMLTSRLWVMMIGSVVLCLLVSFGGIFLSYFWDIPSGATITLLAAGVFVLVMGVQR
                     CVKGI"
BASE COUNT          731 a          635 c          560 g          580 t
ORIGIN      
        1 atgcagatga ggtcccgtac aacccttgca gctttatcac gtttttttgc agttcacctc
       61 cgtaaactgc ttacattcat aaatcaactt aagagtaaat aaatgtatcg gtgttggaaa
      121 agagaaaaac actgaaaaac gggcctggag ggattcgaac ccccggcatt cgggttagaa
      181 gcccgacgct atatcctggc taagccacaa gcccatgact taatgcctta ttctatttgc
      241 ggttccagat aataaactct cctcaaaaat atttctgaaa aagggggatt atcttacccg
      301 cggacagctg cattccgcaa cttccacagt taaaacgtga cagtttctac gttcgaccac
      361 ctttctaatg tgggatgtac aggcaaattt catctgtaag cgcaccgaaa ggcgttcgcg
      421 ttcctcgtag taaatgcccg gagccgcgct tgacatccgc tgcatggtaa ctgaaactgc
      481 cgtgacggtt gcaacaatac ctgcgtcacc agtggtgaga tcctcatgcg gcacagtgga
      541 aacaaaaatg ggatcaccta cggaaacact tgcagcagag ataacattct gagcattgac
      601 gaactctacc gtccgtgccc gccctttttt cagttcgcca ataacctcag gggcaatgcc
      661 cgcaatacct accatctgca tcatgatcac ccgtacatca cctgctctgc agaagagaac
      721 ggcttttgtt ctgcattttc ctgttcctgt acatcatttg ccagctggtg catcatggcc
      781 tcgacctgat ccgcctgttc catgaggggc tgtgtatcaa tcccaaagcc gtagcgggtg
      841 ttaagaattt tcagtgcttc aattgctgca cggggatcaa cattgaagtt tgtctcaacc
      901 agaaaacctg ctgccggaat accgcgaagc tgtgcctcaa taatcatgct cccagatatc
      961 ccagtaatgc ttaatgcggg aaggatgatg gactgctctt tgatcgtttc gagaccatca
     1021 gtggttgttg caaccccaaa gacgcgctca ccatcaccac cggttgtaac accgccgata
     1081 accaccagtt cagttattac agatttaccg gagagccact caatgagacc tgcagagatt
     1141 tcataggata cctgcggggg gatcgggaca tccgcaagaa ctgcaacgaa gttatccttt
     1201 tcgtagagtc ggatgggagc ctgggcaaga ccgtttgttg caagggatat ctgcggaaga
     1261 aatcggctcg taatgtttcc gagatgtgta aatcctgcat tttccaccag gtactgtaag
     1321 gcgatgctgc ccaccagccc tgaacccgga aacccggtta gaagggttat ctcttttgca
     1381 gtgggagcaa gggattctac acgcaccgcg tctgtgatgt ccataaggat agatacatgc
     1441 ctgcaccaat aaatgttctt attgtgacgg tttttaccaa ttgacaatta cttaaattcc
     1501 ggcatcccat agatctgtag tagttatgca ggagtatgaa gttaagcgcg gaatgacaaa
     1561 ggatctccct gaccggattg ctgccgggtt ccccgatatc tttcagatag aaccgaaggt
     1621 cgaggatggg cattactcaa tctcatatgg gagtatgctg cgcctagagg tatgggcagg
     1681 cgaaaagaac aagacggtgt ttgttgatac cgagacaaac aaggatgttc tcgcaatgcc
     1741 tgacgaagag gccgatgtaa tcattcttga caccaacaaa cgcttccgcc agtatcttga
     1801 tctggttacc ggctttacca caaaagagcg ggtaaaacgt gcaaagaaag ccgctgaaaa
     1861 aggagaataa tactttttta cacgttttgc ggtgcaggca agatttctcc ctgcatccag
     1921 caaaaatttg acaaagatga tcacatcatc ttgcagagca agtatcaatc tctgcaaaaa
     1981 ctgaacgttc aaagttgagt gaaaaaatca gatacctttc acgcaccgct gaacacccat
     2041 caccagtaca aacacacctg ccgcaagaag ggtaattgtt gcaccggacg gaatatccca
     2101 gaagtatgac aggaaaattc ctccaaacga tacaagcaga cagagaacaa ccgatccaat
     2161 catcattacc cacagccgtg acgtcagcat acggcatgtt gccgcaggca aagtaagtaa
     2221 tgcaataaca agaataatcc caaccacctg aatcagcatc accaccgcca gtgcgatcag
     2281 gatcagcaga agaagattca gcatcttcac cggcaggttc atcaccgttg catactcttc
     2341 atcaaacgtc accgcaagga gcggctgata caacagtgca acaaccgctg taatcacaac
     2401 cgcaaaaaca ccggcaagaa taagatcgcc gctggtcaca agcagaatat taccaaacag
     2461 gtacgactca atatccggaa catatccagg cgtcagtgca acaaac
//
LOCUS       DALTZZ010000088         1731 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_13274,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000088 DALTZZ010000000
VERSION     DALTZZ010000088.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13274"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            5..>1731
                     /locus_tag="O0X12_01530"
     CDS             5..>1731
                     /locus_tag="O0X12_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJK52873.1"
                     /translation="MTLPLIVQEADSSDVGRGYARINNDVMAKLGVDSGDFVKITGKR
                     TGVAKVMRSSVSGSGGIAIDGDTRRAAGAGIGDTVMVEKTEPQTASKITIQPASQSIK
                     LDSRQLEQFIVQQYAGKPVAKGQVIQIPLATQTRNEDPFFSGWGGFSSYSTEYYAFVI
                     SDVSPGDIAIIGSQTSVHYKDSVYKGEDAPKGKSAGNIHYEDIGGLGRELSQVREMIE
                     YPLRHPEVFEKLGIEPPKGVLLYGPPGTGKTLIARAVANEAGAYFDTISGPEIVSKYY
                     GDSEEKLREIFQKAEENAPAIIFIDEIDSIAPKRDESKGEMERRVVAQLLSLMDGLKN
                     RGKVIVIAATNLPDSIDPALRRGGRFDREIEIGVPDKEGRKEILQIHSRNVPLSENVD
                     LNKYANTTHGFVGADLALVVKEAAMHALRREFPGMNPDETISKEKLEGLKVTAEDFES
                     ALKMVQPSAMREVLVEVPDIHWSDVGGLDSVKEELQQAVEWPLKYADVYKQFATKSPK
                     GFLMFGPPGTGKTLLAKAVANESECNFIAVKGPELMSKWVGESEKGVREIFRKARLAA
                     PSIIFFDEI"
BASE COUNT          429 a          436 c          511 g          355 t
ORIGIN      
        1 tccaataact cttccattaa tagtacagga ggcagacagc agcgatgtcg gtcgcggata
       61 cgcaagaatc aataatgatg tcatggccaa actcggtgtt gactctggtg attttgtcaa
      121 gatcactgga aagcgtaccg gcgttgcaaa ggtcatgcgg tccagtgttt ccgggtcggg
      181 cggaattgca atagatggtg acacccggcg tgcagcaggg gctggtatcg gtgacaccgt
      241 catggtggaa aagactgagc cgcagaccgc atccaagatc acgatccagc cggcatccca
      301 gagtattaaa ctcgacagcc gtcagctgga gcagtttatc gtacagcagt atgccggaaa
      361 accggttgcc aaagggcagg tcatccagat tccgcttgca acccagacac gcaatgagga
      421 tccgttcttc tccggatggg gaggattctc cagctacagc actgaatact atgcattcgt
      481 gatctccgat gtctctccgg gcgacattgc catcatcggt tcgcagacct ccgttcacta
      541 taaagactcg gtctataaag gtgaggatgc gccgaaagga aaatccgccg gaaacatcca
      601 ctacgaggat attggcggtc tgggacgtga actttcccag gtccgggaga tgattgagta
      661 cccgcttcgt caccccgagg tgtttgagaa gctcggcatt gagccgccaa agggagtgct
      721 gctctacggc ccgccgggaa ccggcaagac actgattgcc cgtgcggtag caaacgaggc
      781 aggcgcctac tttgacacaa tctcaggtcc tgaaattgtg tcgaagtact atggtgactc
      841 tgaggagaaa ctccgtgaga tcttccagaa agcagaggag aatgccccgg caatcatctt
      901 catcgatgag atcgactcca ttgcaccaaa gcgtgatgag tccaagggcg agatggaacg
      961 ccgtgttgtt gcccagctgc tttccttaat ggacggctta aagaaccgcg gcaaggtgat
     1021 cgtgattgca gcaaccaatc tgccggactc aattgatcct gctctgcggc ggggaggcag
     1081 gttcgatcga gagattgaga tcggtgtgcc ggacaaagag ggacgcaaag aaattctcca
     1141 gatccactcc cgcaatgttc cgctctccga gaatgttgat ctcaacaagt acgcaaacac
     1201 gacgcacgga tttgtcggag cagaccttgc ccttgtggtc aaagaggctg caatgcatgc
     1261 actgcgccgc gagttcccgg gcatgaatcc ggatgagacc atctcaaagg agaagctcga
     1321 gggtctgaag gttaccgccg aggactttga gtcggcactg aagatggtgc agccgtctgc
     1381 gatgcgtgag gtgctcgtcg aggtgccgga tatccactgg tcggatgtcg gaggacttga
     1441 cagcgtaaaa gaggaactcc agcaggcggt ggagtggccg ctgaagtacg cggatgtgta
     1501 caagcagttc gccaccaaat caccaaaggg attcctgatg ttcggaccgc ccggaaccgg
     1561 taagacactg cttgcaaagg cggttgcaaa cgagtccgag tgtaacttca ttgcggtcaa
     1621 aggtccggaa ctgatgtcga agtgggtcgg ggagtccgaa aaaggcgtgc gggagatctt
     1681 ccgcaaggca cggcttgcag caccgtcgat catcttcttt gatgagatcg a
//
LOCUS       DALTZZ010000089         5082 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_13287,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000089 DALTZZ010000000
VERSION     DALTZZ010000089.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13287"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            167..1270
                     /locus_tag="O0X12_01535"
     CDS             167..1270
                     /locus_tag="O0X12_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52874.1"
                     /translation="MTTQDLLITRISDQIREEQDYTKILEKGGLRLARTKYWVPVPVL
                     LFLIWAVVCITNGISALTILMLWIVGSFFIYMFYFFLMMIPSLGSDSSSHLTSTHTQI
                     QIREFLRLANLLKTVRKNKVTFIEIFWNAFLINAKPLAKGFAIIYLIDMVCAVILYAE
                     EMIDLHLLIIVCFQILLIFAFYAKIVRSKPDTPGFFVPRNQENVSPETETAKIKIWLY
                     ISLFAMITGLLVVGAMLFPGITLNNYLSVISILPSEYPMILILVLLVQAVIIRYFQGI
                     ESRTLMETLNASHIAALKDDLLPRVKAAGPEQLNDLQREFLILRMNKLMVQEFFHRFP
                     VYAIVPNFLLIADPEAQEVLDSTGDEKSIKDLL"
     gene            complement(1297..1797)
                     /locus_tag="O0X12_01540"
     CDS             complement(1297..1797)
                     /locus_tag="O0X12_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HJK52875.1"
                     /translation="MPILYDTPRLKIETKPVHLPNGRDREYLFVQPVSAVCILPTDAE
                     SVYLIRQYRAVIDSYIYEVPAGGMESDDADPITAARRELAEEARFSAGELIPRGFVYS
                     SPGFCTEKLWLFEARDLSPCEEFARDEDEIIEVVRVAKSEAVAMVMDGRICDGKTIAL
                     LMRCLM"
     gene            1908..2288
                     /locus_tag="O0X12_01545"
     CDS             1908..2288
                     /locus_tag="O0X12_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52876.1"
                     /translation="MTAEILTTEQILFALICWICGGIFLIVSIVAYRSKRAVSFYAQM
                     PVAENRITNIPAYNRENAVLWAAYSTPYFLIGFVGTTADLSISGILLVIVCVGSIPAL
                     ILAYQRIYRKYSGEPSVKKDQRRA"
     gene            complement(2273..2944)
                     /gene="queC"
                     /locus_tag="O0X12_01550"
     CDS             complement(2273..2944)
                     /gene="queC"
                     /locus_tag="O0X12_01550"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HJK52877.1"
                     /translation="MKKAVCLLSGGMDSTTLAYVAKDMGYEILALHMNYGQRTERKER
                     ECAKKIANRLNAVDFVEISLDYFTKFGASSLTDMRIPVEEGTVGKADHPNTYVPFRNA
                     NLIAIATSYCEARDGDAVFIGVQSGDHIGYPDCTPQFIDAMQKVISIGTQTDKEIRLL
                     TPFVSMTKTDILREGLRLGVPYEDTWSCYDTEETSCGVCSSCLSRLKAFSDLGVEDPI
                     PYARR"
     gene            complement(2941..3558)
                     /locus_tag="O0X12_01555"
     CDS             complement(2941..3558)
                     /locus_tag="O0X12_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJK52878.1"
                     /translation="MRVTETFVSLQGEGLRQGMPCMFVRFAGCNLHCAWCDTRYSLND
                     SQGVEMTPEEIMQAALESGMRYVCITGGEPLLQKEDLVPLLAAMHEADVLVDIETNGT
                     VPFDDVQEFASVCMDVKCPSSGEMSDLSLLDQLRPTDSVKFVIGDEADYLYMVEVLAA
                     HPHLSAPVCITPVFGTDVQWLIETIISEKLPVRFQLQLHKAVNIP"
     gene            complement(3555..3962)
                     /locus_tag="O0X12_01560"
     CDS             complement(3555..3962)
                     /locus_tag="O0X12_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HJK52879.1"
                     /translation="MSTRIYKETHFDASHRLMHYEGKCSRLHGHRWEVEVWMEGTIDA
                     TSKILLDYNIIKQVVEVYDHQVILNKDDPMVKALSPFQLPVLTDGDPTSELLAEDIRE
                     RLNRFCGEAGVDARVTKIRVWESDGCYAEVDAA"
     gene            4334..4762
                     /locus_tag="O0X12_01565"
     CDS             4334..4762
                     /locus_tag="O0X12_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HJK52880.1"
                     /translation="MGQGKFAARKLVRSAKKFRWSDSVYARRALKLKLKADPLEGAPL
                     GRAIVLEKVGVEAKQPNSAIRKCVRVQLIKNGRQVTAFAVGDGAINFIDEHDEVTICG
                     IGGRAGRSMGDIPGVRFVVIGVNGVTLNELVIGRKEKARR"
     gene            4768..>5082
                     /locus_tag="O0X12_01570"
     CDS             4768..>5082
                     /locus_tag="O0X12_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449775.1"
                     /note="binds directly to 16S rRNA where it nucleates
                     assembly of the head domain of the 30S subunit; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HJK52881.1"
                     /translation="MAEEQTSTSKILLFNKWDTSEVVVKDQGLVRYVTVTSTEVPSSC
                     GRLTQQQFTKSQMAIVERLINRLMQTEANTGKKQLCTRIVMDAFDEINAKTKQNPLQV
                     LVD"
BASE COUNT         1342 a         1469 c         1164 g         1107 t
ORIGIN      
        1 gatcgagata ccggtgataa tttttgcaac cgtgagaaac agattggaaa acaccgacag
       61 acgggcaaca ttctgtttct tggtattatc tgcatccgcg cccataccgc tcacaatgga
      121 agaaactata gggtggcggc acgtaaataa cttaagcggt ctagacatga caacccagga
      181 tctactgatt acccgcatct cagatcagat cagagaagag caggattaca ccaaaatcct
      241 cgaaaaggga gggctcagac ttgccagaac aaaatactgg gttccggtac cggtcctcct
      301 cttccttatc tgggcggtcg tgtgtattac aaacgggatt tccgcgctga ctattcttat
      361 gctgtggatc gtcgggagtt ttttcatcta catgttctac ttcttcctca tgatgatccc
      421 gtctcttgga tctgattcat caagtcatct taccagtacg catacccaga ttcagatccg
      481 cgagttcctg cggcttgcca acctgctgaa aacggtccgg aaaaacaagg ttaccttcat
      541 tgagatcttc tggaatgcat ttctcatcaa tgcaaaaccg ctcgcaaagg gatttgccat
      601 catctatctc atcgatatgg tctgtgcggt catcctgtat gcagaagaga tgatcgatct
      661 gcacctgctg atcatcgtct gtttccagat tctgctcatc tttgcattct atgcaaaaat
      721 tgtccggtca aaacccgaca cgccgggatt ctttgtcccc cggaatcagg agaacgtatc
      781 cccggaaaca gagacggcaa agatcaagat atggctctac atcagtctct ttgccatgat
      841 caccgggctt ctggttgtgg gggcaatgct gtttccggga attacactca acaactatct
      901 ctcggtcatc tccattcttc caagcgagta tccgatgatt cttatccttg tactgctggt
      961 acaggcggtt atcatccggt acttccaggg tattgaaagc cggactctca tggaaaccct
     1021 caacgccagc cacattgcag ccctcaaaga tgatctgctc ccccgggtga aagcggcagg
     1081 accggaacag ctgaacgatc tgcaacggga gtttttgatt ctccggatga acaaactgat
     1141 ggtacaggag ttctttcatc ggttccccgt ctatgcgata gtccccaact tcctgctgat
     1201 tgcagatccg gaagcacagg aggtactcga cagcaccggt gatgaaaaaa gcattaagga
     1261 tctgctctga gcagaataga aaaaagattc accgcatcac atcagacacc gcatcaaaag
     1321 cgcaatcgtc ttcccgtcac agatacgccc gtccatcacc atggcaaccg cctcgctttt
     1381 tgccacacgc accacctcaa taatctcatc ctcatcacgg gcaaactcct cacagggaga
     1441 aagatcccgg gcctcaaaca gccacagttt ctcagtacag aatccgggtg atgagtacac
     1501 aaatccccga ggaatcagtt caccggcaga aaaacgagct tcctccgcaa gctcgcgccg
     1561 ggctgcagtg atcggatcag catcatccga ctccatcccg cctgccggaa cctcgtaaat
     1621 gtacgaatcg atcaccgccc ggtactgacg aatcagatac accgactcgg catccgtcgg
     1681 cagaatacac accgcagata ccggctggac aaagagatac tcccgatcgc ggccgttcgg
     1741 gagatgtacc ggttttgttt cgatcttcag acgaggggta tcatacagaa tcggcatgga
     1801 aaatgctcac tgaaactcca ttggttgtgg agaaataaaa accctcagac agaataaagt
     1861 agcagacccg ccatacacat aaccagaaac ccgtccgcga ggcccagatg acagcagaaa
     1921 tcctcacaac cgaacagatc ctatttgccc tcatctgctg gatctgcgga ggaatctttt
     1981 tgatcgtgtc catcgttgcc taccgcagca aacgggcagt atccttttac gcacagatgc
     2041 cggttgcaga aaaccgcatc accaacatac ctgcatacaa ccgggaaaat gcggtcctct
     2101 gggcggcata cagcaccccg tacttcctca tcggatttgt tgggaccact gccgacctct
     2161 caatcagcgg aatactcctt gtgatcgtct gtgtcggaag tatcccggca ctcatccttg
     2221 cctaccagag aatataccgg aaatactccg gcgaaccatc cgtaaaaaaa gatcagcggc
     2281 gggcataggg aatcggatcc tcaacgccca ggtcggaaaa cgccttcagc cgtgacaaac
     2341 acgaactgca tacaccgcag gaggtctctt ctgtatcata gcacgaccag gtatcctcgt
     2401 acggaacgcc aagccgcaga ccctcacgca ggatgtccgt ctttgtcatc gacacaaacg
     2461 gcgtcagaag ccggatctcc ttgtcagtct gcgtcccgat ggagatcacc ttctgcatcg
     2521 catcaataaa ctgcggcgta cagtcaggat acccgatgtg atcaccggac tgaaccccga
     2581 taaacaccgc atcgccgtcg cgtgcctcac agtacgacgt tgcgatcgca atcagatttg
     2641 cgttccggaa cggtacatag gtgttcggat gatccgcttt cccgacggtt ccctcctcca
     2701 ccggaatacg catatccgtc agactgcttg caccaaactt cgtaaaataa tccagggaga
     2761 tctccacaaa gtccaccgca ttcagacggt ttgcaatttt ttttgcacac tcccgctcct
     2821 ttcgctctgt ccgctgaccg tagttcatgt gcagggccaa aatctcatag cccatatcct
     2881 ttgcaacgta tgcgagagtc gtcgaatcca tcccgcccga aagcagacac acggctttct
     2941 tcatggaata ttcaccgcct tgtgcagctg cagctggaat ctgaccggaa gtttttctga
     3001 aatgatcgtc tcgatcagcc actgcacatc cgttccgaac accggcgtaa tacagaccgg
     3061 cgccgaaaga tgtggatggg ctgcaagaac ctccaccatg tacagatagt ccgcctcatc
     3121 cccaatcaca aacttcaccg agtccgtggg cctgagctga tccagcagag acagatcact
     3181 catctccccg gacgacggac acttcacatc catacagaca gaagcaaact cctgcacatc
     3241 atcaaacgga accgttccat tcgtctcaat atccaccagg acatccgcct catgcatcgc
     3301 cgcaagcagc ggtacaagat cctccttctg aagcagcggc tctcctcccg taatacagac
     3361 ataccgcatc ccagactcaa gtgccgcctg catgatctcc tcgggcgtca tctcaacacc
     3421 ctgtgaatcg ttcagcgagt atcgggtatc acaccaggca cagtggagat tgcaccctgc
     3481 aaaccgcaca aacatacacg gcatcccctg ccgcagtccc tcaccctgca agctcacaaa
     3541 cgtctcggtg actctcacgc tgcatccacc tctgcatagc agccgtccga ctcccagacc
     3601 cgtatctttg tcacccgggc atcaacgccc gcctcgccgc agaaccggtt cagccgctcg
     3661 cgaatatcct ccgcaagcag ctcactggtc ggatcgccgt ccgtcaaaac cggcagctgg
     3721 aacggagaga gggcttttac catcggatca tccttgttta agatcacctg atgatcatac
     3781 acctccacca cctgtttgat gatattatag tcaagaagga tcttcgacgt cgcgtcaatc
     3841 gttccttcca tccagacctc aacctcccag cggtgaccgt gcagccgaga acattttccc
     3901 tcgtaatgca tcagccggtg agacgcatca aaatgtgttt ccttatagat ccgggtactc
     3961 atagttgacc acatattgct cgcgggaaag tataataata gaggtaaaga gaagaaagga
     4021 gaacaaacgg gacagtaaag acggatgaca acaaacgatc ccgcagcttt gctgaagccg
     4081 gaaaaaccgg cgggagtact gctctcttcg ggcacaatct cccattaata ttattaggac
     4141 agcctgctca taagtatagg aacatgcaag ggaaggctat ctctcaagac cctgccctcg
     4201 cattcacaat attccaacag aggaatagtt tcccgtaaat ctacgtgcac gtttttttcc
     4261 gggaaacaca gcacaggaga ctgtgttaca agattcatct atccaaaaaa tctactgtaa
     4321 ctgaggtaat ttaatgggac agggaaaatt cgcagcaaga aagcttgtcc gttccgccaa
     4381 gaaattccgg tggagcgact cagtttacgc gcgcagagcg ctcaaactga agctgaaggc
     4441 agacccgctt gagggcgcac cgctcggacg cgcaatcgtc ttagagaagg ttggtgttga
     4501 agcaaaacag ccgaactccg caatcagaaa gtgcgttcgt gttcagctga tcaaaaacgg
     4561 ccgtcaggtc actgcattcg cagtcggcga cggtgcaatc aacttcatcg atgagcacga
     4621 cgaagtcacc atctgtggta tcggcggtcg tgcaggacgt tccatgggag atattccggg
     4681 agtccgtttc gtcgttatcg gcgtcaacgg tgtcacgctc aacgagcttg ttatcggacg
     4741 caaagagaag gcacggaggt aaatagtatg gcagaagaac agacttcaac cagcaagatc
     4801 ctgctgttca acaagtggga taccagcgaa gtcgtcgtca aggatcaggg tctcgtccgt
     4861 tacgtgaccg ttacctccac cgaggtccca agctcctgcg gcagactcac ccagcagcag
     4921 ttcaccaaga gccagatggc aattgttgag cggctgatca accgtctgat gcagacggaa
     4981 gcaaacaccg gcaagaaaca gctgtgcacc cgtatcgtca tggatgcatt cgacgagatc
     5041 aacgcaaaga ccaagcagaa cccgcttcag gtccttgttg ac
//
LOCUS       DALTZZ010000090         2790 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_13412,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000090 DALTZZ010000000
VERSION     DALTZZ010000090.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13412"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="O0X12_01575"
     CDS             complement(<1..281)
                     /locus_tag="O0X12_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32"
                     /protein_id="HJK52882.1"
                     /translation="MPSVPELLAPAGSPEALKAAVAAGADAVYLGGKSFGARRYAANF
                     DREELGQAVLYAHKNGVKVYVTVNTLATDSELPGIAEYLIFLSEIGVDAV"
     gene            380..988
                     /locus_tag="O0X12_01580"
     CDS             380..988
                     /locus_tag="O0X12_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HJK52883.1"
                     /translation="MITLGEDFKPALKYKPYLAIYTMVAVAIIWAACFGWIVFLGETD
                     IPQTIVVAIIAVLVICLLFVLLWTVLYYKSVVYHLNATEMTWKRGVWFRKTGIVPYNR
                     ITNVDIVQGPVMRLFGISNLKIQTAGYSGGNGSAEIALEGIEEPEPLRALIMDFVRSG
                     SAGVAATGVDVAEETQAVSGDADIKALLAEVAAIRKLLEERK"
     gene            1159..1380
                     /locus_tag="O0X12_01585"
     CDS             1159..1380
                     /locus_tag="O0X12_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52884.1"
                     /translation="MVVPCLTCTALDKHEYTLNQATGFYEYHITCSRGISTPDPMPKE
                     GFTCEQYENKLAVLPRVRRSSCAIEVHRD"
     gene            complement(1489..>2790)
                     /locus_tag="O0X12_01590"
     CDS             complement(1489..>2790)
                     /locus_tag="O0X12_01590"
                     /inference="COORDINATES: protein motif:HMM:NF021370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HJK52885.1"
                     /translation="GIIFGVYLFGTGYLIPAVLSILVFSLAIITFLLLFDRPLRKAHG
                     ISAMQFINAFLAHLTDGSKGLEEYFRTIGEAVTVPETSFFFRREGKKDVLFVVPNLHP
                     GPLSEVGGGNYPKHLHDMFSPQETVFVSHGCATHDFNLVSESECEKIAEAIDETRADL
                     SYTPLAGRPMRNRFGTVSVLSQRFGRSILLVTTRSPEMTEDLDYAVGAIVMAEGHRWY
                     ENVGFVDAHNCMVEVTSVVLPASHTGSEYIRASTEAMEQQADAELSEFSIGVSQKVLP
                     FSREQGFGDMGVQVLVTEVAGTKTAYVLFDGNNVHMGAREVLRDAVIATGVTDAEILT
                     TDSHVVNTISGRNPVGMAVPAEEILPYLLDAVAEAVADLSPAHVASATGMCRDVVVFG
                     PSRTVQLSATVNAMVINLLPLSVLLLLASILITLLIFLFFS"
BASE COUNT          703 a          737 c          686 g          664 t
ORIGIN      
        1 actgcgtcca cgccgatttc agagaggaag atgagatatt cagcgatccc cggaagttca
       61 ctgtcggttg caagagtgtt gacggtgaca tagaccttaa caccgttttt gtgtgcgtag
      121 agaacggcct gaccgagctc ctcgcgatca aagtttgcag cataccggcg ggcgccaaac
      181 gactttcctc cgagatagac cgcatcagcc cctgcggcaa ctgctgcttt gagagcttca
      241 ggggatccgg cgggtgcgag gagttcggga acagaaggca tagatgtgat gtagtattgg
      301 ttgtgcgaga ggttatagat taaggagact atgcttgagg aagaaagttt ttcttatgac
      361 agtgagatgt tacttagcaa tgatcacatt aggtgaagat ttcaaaccgg ctctgaaata
      421 caagccgtat cttgccatct acacaatggt ggcagttgcc attatctggg cagcctgttt
      481 cggctggata gtcttcctcg gcgaaacaga cattccccag acgatcgttg ttgcgatcat
      541 cgctgtcctt gtcatctgtc tgctgttcgt tctcctctgg acggtgctgt actacaagtc
      601 cgtcgtgtat catctcaatg caaccgagat gacctggaaa cgcggggtgt ggttccgcaa
      661 gacgggaatt gttccctaca accgaatcac caatgttgat atcgttcagg gtccggtgat
      721 gcgcctcttt ggtatctcca acctcaaaat ccagacggca ggctactcag gcggtaacgg
      781 atccgcggag attgcccttg aaggaatcga agagccggaa cccctccgtg ccctgattat
      841 ggactttgtc cgcagcggtt ctgcgggtgt tgcggcaacc ggcgttgatg ttgcggaaga
      901 gactcaggca gtgtctggag atgcagatat caaagccctc cttgcagagg ttgcggcaat
      961 ccgtaaactc cttgaggaga gaaaataatt cttttctttt ttcagaatct tttttggatt
     1021 tttgtttcgg tatggtccat ggttttttgc catactgcac acccgatcgc acacaaagaa
     1081 tcctgttcac gcttttcgga aatccgcaag aaaaaacact tcgttatctt catacgctcc
     1141 gcagtacatc tatgtatcat ggtagttccc tgtctcacct gtactgcact tgataaacac
     1201 gagtacaccc tcaatcaggc aaccggtttc tatgaatatc atataacctg tagccgtggg
     1261 atttccacac ctgaccccat gcccaaagag ggatttacct gcgaacagta cgaaaacaaa
     1321 cttgcagtcc tgccgagggt gcgccggagc agttgtgcca tagaggtaca ccgcgactaa
     1381 tgagaaaaaa acaccaggca ctcctgcagt atcatgcagg atcatttttc gttttttgtc
     1441 gggagcacag ctcccgaaaa ctaatgagag atcgggttac ccccgaactc agctgaagaa
     1501 cagaaagatc agcagtgtta tgaggatgga tgccagcagc agaagaaccg aaagcggcag
     1561 aagattgatt accattgcat ttaccgttgc agacagctgc actgtcctgc tcggcccaaa
     1621 caccaccaca tcgcggcaca taccggttgc ggaagcaaca tgtgcaggtg acagatccgc
     1681 aaccgcctct gcgaccgcat caagcaagta gggaaggatc tcctctgcgg gaaccgccat
     1741 gcccacagga tttcttccgg atatggtgtt taccacatgc gaatcggttg tcagaatctc
     1801 tgcatcggta acacctgttg caataactgc atcccgcaga acctcacgcg cacccatgtg
     1861 cacattgttt ccgtcgaaaa gtacgtaggc tgtttttgtt ccggcaacct ctgtcaccag
     1921 tacctgaacg cccatgtcgc cgaaaccctg ttcacgggaa aacggcagaa ctttctggga
     1981 aacaccgatg gaaaactctg agagctctgc atccgcctgc tgctccatcg cctctgtgga
     2041 cgcacggatg tactcgcttc cggtatgtga tgccggcagc acgactgatg ttacctcgac
     2101 catacagtta tgcgcatcca caaacccgac attctcatac cagcggtgac cttcagccat
     2161 tacgattgcc ccgaccgcat aatcgagatc ctctgtcatc tccggggacc gcgtcgtcac
     2221 cagcagaatc gatctaccaa acctttgtga cagaacagaa acggttccaa atctgttcct
     2281 catcggtctg cctgcaagag gtgtatagga aagatccgcc cgtgtttcat caattgcttc
     2341 tgcaatcttt tcgcactcgc tttccgaaac aaggttgaaa tcatgtgttg cacagccgtg
     2401 tgagacaaac actgtctcct gtggggagaa catatcatgc agatgtttgg gataatttcc
     2461 gccgccgacc tcggagagcg ggcccggatg cagattcggc accacaaaca ggacatcctt
     2521 ttttccctct cgccggaaga agaaacttgt ctcagggacc gtcaccgcct ccccaatcgt
     2581 ccggaagtac tcctccaggc ctttggatcc atccgtcaga tgtgcaagaa acgcattgat
     2641 aaactgcatc gcagatatcc cgtgtgcctt gcgcagcgga cgatcgaaga gaagcaggaa
     2701 ggtgatgatt gcaagggaaa agacaagaat tgataagaca gccggaatca gatatcctgt
     2761 tccaaacaga tacaccccga aaattattcc
//
LOCUS       DALTZZ010000091         4377 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_13422,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000091 DALTZZ010000000
VERSION     DALTZZ010000091.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13422"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(21..590)
                     /locus_tag="O0X12_01595"
     CDS             complement(21..590)
                     /locus_tag="O0X12_01595"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HJK52886.1"
                     /translation="MIIVGVGAGSGMLTEDGISRIRTARVIYGSGRALDLARGYAAAD
                     AKLILITDYKALRSLPDDAVVLSTGDPLMAGLGYLPGEVVPGISSMQVAFARLKVPWT
                     NVAVVNAHGKDHTVAMERAVRDVAAGHVVFIIADPDFSLAALGSALPPDVRLAVCEDL
                     GYPSERVAEGTAACPPEVRSRLFCVVAGY"
     gene            complement(587..1609)
                     /locus_tag="O0X12_01600"
     CDS             complement(587..1609)
                     /locus_tag="O0X12_01600"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HJK52887.1"
                     /translation="MIDPVSGFLYPEAWVRLCTDDAERELVLSGFGVLTANGSVLRRG
                     FTTGTTAAAAAFAAVASLRMTQVSEVEILLPCGITAQVPVLGRNGHGEARKFSGDYPD
                     DVTAGILIVAEAQVSDEISLVAGEGIGRFVRETPRYGLGEPAISPPARAEILGAIEAA
                     CRMAGISGAAVVLRIPEGERVGSQTLNPKVGVCGGISVVGTTGFVEPWDDHLTDTLGE
                     RIAAASDVVITTGRTGLRFSRLLFPEFEVVLAGSKLSEALSAAKNCRHVVVCGLPGLI
                     LRFFHPEVATSRGFSTVEELAASAEGPAVLKAEVTDAVQRFPGVRIVLLDRDGEVLVD
                     SEEGVR"
     gene            complement(1606..2265)
                     /locus_tag="O0X12_01605"
     CDS             complement(1606..2265)
                     /locus_tag="O0X12_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HJK52888.1"
                     /translation="MSKELLRPAVTTESTYTDIGADTPEGFSISSRSRSLAREAVGNS
                     TPEDRIRQRCSIAVGDWAMADLLRFSHDPVAAGLAALDAGAPIFTDIRMVLTGIQKRG
                     HTCPVSCALDFGADISRNQGITRSSAGFVALRDELEGSIVVIGNAPSALLTVCGFVDE
                     GICPALIVGTPVGFVNAAESKEVLRTKSVPSISNVGTRGGTPVAVASLNEIITMYAEQ
                     V"
     gene            complement(2213..2974)
                     /gene="cobJ"
                     /locus_tag="O0X12_01610"
     CDS             complement(2213..2974)
                     /gene="cobJ"
                     /locus_tag="O0X12_01610"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HJK52889.1"
                     /translation="MGTLWIVSSGPGSLQQLTPAAMKAIAAADVVIGNAFYLDMLEVL
                     LKGKQVIRSSMGKEVDRAKEAARLAADCNVAMITGGDAGVYGMASIVLEVVEHEFPGT
                     DVEVLPGVTAATAAASRLGSPLSGDYVTLSLSDLLTPWELIEKRLHLAFQMGVPVALY
                     NPKSRGRPLNLGKAIQIALQYRSPETPVGVVRNVFRSGEETFCVTLASLGENDELVDM
                     HSILIIGGDETRFWEENGDVKGIITPRGYHRKYVY"
     gene            complement(2977..3846)
                     /gene="cbiG"
                     /locus_tag="O0X12_01615"
     CDS             complement(2977..3846)
                     /gene="cbiG"
                     /locus_tag="O0X12_01615"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HJK52890.1"
                     /translation="MKTAVVVLDRFRSEGERVAEFLDAELVPYSDHVFADLYSRVEVI
                     VAVMSAGIAVRGCAPHLSDKWHDPAVVVVTPDLRYAIPVLGGHHGGNVCAKRLAALGI
                     EPVISTATETRGRPSVEETARTGGFVVVNRSSTREVNGAVLDGDVPVVRVDPPHIVVA
                     NPGVSVLVNESRYVIGVGCRLGTTAEEIVAAVNAACSAAGISHEVVKMYATTIKKFHE
                     KGLHEAVRSLSGNLIFLDDETINAQVPRTPSRAGMLGLAGVAEPCALALAKSGTLILN
                     KTVYGRVTIAIGK"
     gene            complement(3843..>4377)
                     /locus_tag="O0X12_01620"
     CDS             complement(3843..>4377)
                     /locus_tag="O0X12_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt-precorrin-4/precorrin-4
                     C(11)-methyltransferase"
                     /protein_id="HJK52891.1"
                     /translation="ITSVISENVRAGKLVVRLHSGDPAIYGSIVEQIAPLEEDGIFAE
                     IVPGVSSMFGAAAALLTEYTLRGVSESVIVTRSAGETLDSDQLAELSEHETTMVIFLS
                     TGHIDSVMKKLRRSPDTPVAVVYHASWPDQQVIRGTIADIAEKVHAAGIERSALIIVG
                     DVVRGVKAAYTNSHLYG"
BASE COUNT         1175 a         1406 c          931 g          865 t
ORIGIN      
        1 caaaataaga aaaagaaaaa tcagtatcct gcaaccacac aaaacagcct gcttcggacc
       61 tccggcggac acgcggctgt cccctccgca acacgctcgg acgggtaccc aagatcttca
      121 cagactgcaa gccgtacatc cggagggagc gcggagccga gtgccgcaag agaaaaatca
      181 ggatccgcaa ttataaacac aacatggccc gcagccacat cccgcaccgc acgttccata
      241 gcaacggtat gatctttccc atgggcattc accaccgcaa catttgtcca cggaaccttc
      301 agtctggcaa acgccacctg cattgacgaa attcctggca ctacttctcc aggaagatat
      361 ccaagtcccg ccatcagggg atcgcccgtt gataatacaa ctgcgtcatc aggaagagat
      421 cgcaacgcct tataatcggt aatcaaaatc agctttgcat ccgccgctgc atatcctctg
      481 gccagatcaa gcgcacggcc cgaaccgtag ataacccgtg cagtacgaat gcgggaaatt
      541 ccatcttcag tcagcatccc ggaaccagca ccaacaccga caatgatcat cttacaccct
      601 cctcggaatc aaccagtacc tctccgtcac gatccagcag tacgatacgc acccctggaa
      661 accgctgcac cgcatctgtc acctcggcct ttaatacagc cggaccctca gcggaagccg
      721 ccagctcctc caccgttgaa aaaccccggg acgttgcaac ctctggatgg aaaaaccgca
      781 gaataagacc cggtaggccg cacacaacga cgtgacggca gttctttgcc gcagacagag
      841 cctctgaaag cttagatccc gcaagcacca cttcaaactc cgggaacaac agtcgcgaaa
      901 accgcagacc tgtccttccc gttgtaatca ccacatccga agctgcggca atccgttcac
      961 caagcgtatc tgtcagatga tcatcccacg gctccacaaa ccctgttgtt cccacaactg
     1021 aaattccccc acacacgcca acctttgggt tcagcgtctg gctgccaact ctctcaccct
     1081 caggaatccg taaaacaacc gctgcaccgg agatcccagc catacgacaa gctgcctcaa
     1141 ttgctcccaa aatctctgca cgtgccgggg gactaattgc aggttcacca agtccatacc
     1201 gcggggtttc acggacaaaa cgcccgatac cctctcctgc gacaagagag atctcatcag
     1261 acacctgtgc ttcggctaca atcaaaatcc ccgcagtcac atcatccgga taatcacctg
     1321 aaaactttcg cgcctcacca tggccgtttc tgccgagtac cggaacctgt gccgttatac
     1381 cacatggaag cagaatctcc acctcagaca cctgagtcat tcgcaatgat gcaacagcag
     1441 caaatgctgc tgctgccgca gtcgtacccg tcgtaaatcc acgacggagt accgaaccat
     1501 ttgcagtcag tacaccgaaa ccagacagca ccagctcgcg ttccgcatca tcggtacaca
     1561 aacgtaccca cgcttcagga tacagaaaac cgctcacagg atcaatcata cctgctcagc
     1621 atacatcgta atgatctcgt tcagcgatgc aaccgcaacc ggcgttcccc cgcgggtacc
     1681 tacatttgag atcgacggaa ccgatttcgt acgaagaacc tcttttgact cagcagcatt
     1741 tacaaatccg acaggggtac ccacaatcaa cgcagggcag atgccctcat ccacaaaacc
     1801 acagaccgtc agaagagcac tcggtgcatt tccgatcacc acgatcgaac cctccagttc
     1861 atcccgtaga gctacgaacc ctgctgaact cctggtaata ccctgatttc gggaaatatc
     1921 cgcaccaaaa tcaagagcac aggaaactgg acaagtgtgc ccgcgcttct gaataccagt
     1981 gagaaccatc cggatgtccg tgaaaatcgg ggcacctgca tcaagggcag caagacctgc
     2041 cgcaacagga tcatgcgaaa accgcagaag atccgccata gcccagtcac ctactgcaat
     2101 cgaacatcgc tgacgaatcc tgtcttcagg cgtcgagttt ccgacagcct cccgtgccag
     2161 gctcctgctg cgcgacgaaa tcgaaaaacc ttccggcgta tcagcaccaa tatcagtata
     2221 cgtactttct gtggtaaccg cggggcgtaa taattccttt gacatctccg ttttcctccc
     2281 agaacctggt ctcatcaccg ccaataatca ggatcgagtg catatcaacc agctcatcat
     2341 tttctccaag cgatgcaaga gtcacacaga acgtttcctc accagatcgg aacacattcc
     2401 gcaccacacc aaccggcgtc tccggggaac ggtactgcaa agcaatctga attgcttttc
     2461 ccagattaag tggtcggcca cggctctttg gattgtacag agcgaccggg acccccattt
     2521 gaaaggcaag atgcagacgc ttctctatca gctcccatgg tgtaagcaga tccgacagac
     2581 tcagggtcac ataatctcct gaaagagggg agccaagacg cgatgctgcc gcagttgcag
     2641 cagtaacacc cgggagcacc tcgacatccg ttcccggaaa ctcatgttcc acaacttcaa
     2701 gcacaatgct ggccatgccg tacacgccgg catcaccacc cgtgatcatt gcaacattgc
     2761 agtcagcggc aagtcgtgcc gcctcttttg cgcgatcaac ctctttcccc attgaactgc
     2821 gaataacctg cttgcctttc agcaggacct ccagcatatc cagataaaac gcattaccga
     2881 taacaacatc tgccgcggca attgctttca ttgcagctgg ggtcaactgc tgcagactgc
     2941 ccggtccaga actaactatc caaagagtac ccatgattat tttcctatgg caatcgtgac
     3001 acggccatag acggtcttgt tcagaatcaa cgtgcctgac tttgcaagtg ccagagcaca
     3061 cggctctgca acgccagcca gacccagcat ccctgcacgg gagggggtcc ttggcacctg
     3121 tgcattaata gtttcatcat ccaaaaagat gagattgccg gataaggacc tcacagcttc
     3181 gtgcagcccc ttctcatgaa acttcttgat cgtcgtcgca tacatcttaa caacctcatg
     3241 cgagatacca gccgcagaac atgcggcgtt cactgccgca acaatctcct cagcagtcgt
     3301 acccagacga catccgacac ctatcacata tctggattca ttcaccagaa cagacacccc
     3361 cgggttggca acaacaatat gaggcggatc aacccggaca accggcacat ctccgtcaag
     3421 aaccgccccg ttcacctcac gggtcgatga ccggttcacc accacaaaac ctccggttcg
     3481 ggcggtctcc tccactgaag ggcgcccacg tgtttcagtc gcagtagaga ttaccggctc
     3541 aatgccgagt gcagcaagac gctttgcaca gacattccct ccatgatgcc ctccaagaac
     3601 aggtatcgca taccgaaggt ccggtgttac cacgacaacc gcaggatcat gccacttatc
     3661 agacagatgc ggcgcacaac ctcggaccgc aatcccagcc gacatcaccg caacaatcac
     3721 ctctaccctg ctgtacagat ccgcaaacac atgatccgaa tacggcacca gctccgcatc
     3781 aagaaattcc gcgacacgtt ctccctcgga acggaatcga tccagaacaa cgacagcagt
     3841 tttcatccgt agagatgaga gttcgtgtac gcggctttga caccccgcac cacatcaccc
     3901 acaataatca gcgcagaacg ttcaattcct gccgcatgta ctttttcagc aatatctgca
     3961 atagttcccc ggataacctg ctgatccggc cacgacgcat gatacaccac cgcaaccggt
     4021 gtgtcaggac tccggcgaag ttttttcatc accgagtcta tgtgaccggt tgaaagaaaa
     4081 atcaccatgg ttgtttcatg ctccgacagt tctgcaagct gatcagagtc aagagtctct
     4141 cctgcggatc gggtgacaat cactgactcg gacacgcctc taagtgtgta ctctgtcagg
     4201 agcgccgctg ctgcaccaaa cattgaagaa acacccggta caatctccgc aaatatcccg
     4261 tcctcttcaa gaggggcaat ctgttccaca atcgatccat agattgctgg atcaccagag
     4321 tgcaatcgta ccactagttt tccggcccgt acattttccg aaatcactga ggtgatc
//
LOCUS       DALTZZ010000092         1855 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_13459,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000092 DALTZZ010000000
VERSION     DALTZZ010000092.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13459"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="O0X12_01625"
     CDS             complement(<1..810)
                     /locus_tag="O0X12_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HJK52892.1"
                     /translation="MYNETLLMMPGPVPMPESVRNAMTKQAINHRSKEFGDCYADIVR
                     TLKPVFGTKNDILVLSGSGTAGQEAAIGSFAKGKKVASLVNGKFGERLGQIAAIYGES
                     VMIESEWGHPLNLEGLKEALENGAEVVTLVHNETSAAILNPAEEVGKLARKYDALFIL
                     DAITSIGGDVVEADKWGADVAIVGSQKCLAAPAGLAAVSVSQRAWDRLSETRPFYLDL
                     KKAKKSADGNPMETPTTPAVPLFLALREACRLIEEEGIEHRIARHHKMSAAV"
     gene            860..1252
                     /locus_tag="O0X12_01630"
     CDS             860..1252
                     /locus_tag="O0X12_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HJK52893.1"
                     /translation="MADVAVIAGSASDQAIIDKVVNTLAQYSISYDMQILSAHRDAEK
                     LDNYVKTSDAKVFICIAGMSAALPGVVAARTPKPVIGVPVSGKIAGGLDALLSIAQMP
                     KGVPVACMAVDGGENAAHMAHRILSLLP"
     gene            complement(1386..>1855)
                     /locus_tag="O0X12_01635"
     CDS             complement(1386..>1855)
                     /locus_tag="O0X12_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HJK52894.1"
                     /translation="GVQRSCSADQWMQKSEDEGGLFGEEKYNQLVKDGNIKLYDSFPM
                     SMVALEQGLVDVVIFDDANVENHIKGKSQFAIVGVIETGEYYAVAVRKDDNDLRELMN
                     EGLTELMASDKWDELIAQYIVEDVAEPTVEPTTNETIVENVTAVETTPAETTA"
BASE COUNT          402 a          470 c          466 g          517 t
ORIGIN      
        1 gaccgcggca gacattttgt ggtggcgtgc aatgcggtgt tcgattccct cttcctcaat
       61 aagtctgcat gcttcacgga gcgcgaggaa gagcggaact gccggcgttg tcggtgtttc
      121 catcgggtta ccgtcggctg atttctttgc cttctttaag tcgagataga agggacgtgt
      181 ttcagaaagg cgatcccatg cacgctggga tacggaaact gcggccagtc ctgcaggagc
      241 cgcaaggcat ttctgggatc cgacgattgc aacatccgct ccccatttat ccgcctcgac
      301 aacatctccg ccgatcgagg tgattgcatc aaggatgaag agtgcatcgt atttacgtgc
      361 gagtttaccc acctcttcag cagggttgag gattgcggca gaggtctcat tgtgaacaag
      421 ggttacaacc tctgcaccgt tttccagggc ttccttgagt ccttccaggt ttaacgggtg
      481 tccccactcg gactcgatca tcacggattc tccgtagatt gcggcaatct gtccgagccg
      541 ctctccgaac tttccgttta cgagggatgc gactttcttg cctttggcaa aggatccgat
      601 tgcggcttcc tgtccggcgg ttcctgatcc tgagagaaca agtatatcgt ttttcgtacc
      661 gaacaccggt tttaaggtcc tgacaatgtc tgcgtagcag tcaccgaact ccttgctgcg
      721 gtgattgatt gcctgctttg tcattgcgtt tctgacggac tccggcatcg gaaccggtcc
      781 cggcatcatg agaagtgttt cgttatacat ggtatcaggg atgtttgtac atatatgtga
      841 agagacaata cttatattca tggcagatgt tgcagttatt gccggatccg catcagatca
      901 ggctattatc gataaagttg tgaatacgct cgcgcagtat agcatctctt atgacatgca
      961 gattctctct gcccaccgtg atgcggaaaa gctggacaac tatgtaaaga caagcgatgc
     1021 aaaagtgttc atctgtattg caggaatgtc tgcggcactt ccgggtgttg ttgcggcaag
     1081 aacaccaaag ccggtgatcg gtgtgccggt gtcgggaaag attgccggag gccttgatgc
     1141 cttgctttct attgcgcaga tgccgaaagg tgttcctgtt gcctgtatgg ccgttgatgg
     1201 cggggagaat gctgcacaca tggcccaccg gatcttatcg cttcttccct gataatctta
     1261 catcactttt ttcctgaatg gtcagctatt attgatcaag ctgaacacat tttctcttgg
     1321 aatagctgtc ctgatcttgt ggacaggtaa aaaaagaaaa tctgcgaagg cagattatct
     1381 cacgcttatg cggtagtctc tgctggagtt gtctcgacag cagttacgtt ttccacaata
     1441 gtctcgtttg tcgtcggttc aacggtcggc tcagctacat cctctacgat gtactgtgca
     1501 atcagttcat cccacttgtc ggatgccata agttcagtga gaccttcgtt catcagctca
     1561 cggagatcgt tgtcatcctt acggactgca actgcgtaat actcaccggt ctcaatgacg
     1621 ccaactatgg cgaactggct ctttcccttg atgtggttct cgacatttgc atcgtcgaag
     1681 ataaccacat caacaagtcc ctgctcaagt gcgaccattg acatcgggaa ggagtcatac
     1741 agcttaatgt tgccgtcctt taccagctgg ttgtacttct cctctccgaa aagtccgccc
     1801 tcatcttctg atttctgcat ccactggtcg gcggaacagc tgcgctgtac accaa
//
LOCUS       DALTZZ010000093         2769 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_13685,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000093 DALTZZ010000000
VERSION     DALTZZ010000093.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13685"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="O0X12_01640"
     CDS             complement(<1..416)
                     /locus_tag="O0X12_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HJK52895.1"
                     /translation="MTKVMICGALGRMGTTIANMVVENPDLELVGGVDIRPGQILGKP
                     VVESSCLAAFIEEQKPDVMIDFTVAAATMVNAKIAAAHGVALVIGTTGFTPEQDAELL
                     DAVKGVPVVKTTNFSVGVNIFWELVREAAKRLGDYD"
     gene            complement(409..1302)
                     /gene="dapA"
                     /locus_tag="O0X12_01645"
     CDS             complement(409..1302)
                     /gene="dapA"
                     /locus_tag="O0X12_01645"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HJK52896.1"
                     /translation="MFEGVIPALITPFREDPSQSLDIEGLRSCIEHVISGGVHGLLPC
                     GSTGESATMTHAEHIRVIEEAVSAANNRIPVIAGTGSNNTEEALLMTRAAKIAGADGA
                     LLISPYYNKPNRSGLLKHYKKVADVGLPVVVYNIPGRTGQNLPPDLIIEMAKTIPNIV
                     AVKEASGNIDQMMAIIHGLEGVEREYPFVLTSGDDSLTLPVLSIGGQGCISVTANIEP
                     KRMVAMYDAFVHGDMDKARKIHYELMELSKALFMDVNPVPVKRAAEIRGLIASGSVRL
                     PLDSLNPQMTEKLREVLSHYD"
     gene            complement(1506..1700)
                     /locus_tag="O0X12_01650"
     CDS             complement(1506..1700)
                     /locus_tag="O0X12_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HJK52897.1"
                     /translation="MGIKPSYIKTMGTALMEQHGASFNNNFDDNKRVVSEVTTIESKI
                     IRNRVAGFITRKVNTNNRRR"
     gene            complement(1711..2223)
                     /locus_tag="O0X12_01655"
     CDS             complement(1711..2223)
                     /locus_tag="O0X12_01655"
                     /inference="COORDINATES: protein motif:HMM:NF021625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52898.1"
                     /translation="MSAKEQSVILKALQQAHGRVMNIPRYLLPSGGVALAYAVPNPRD
                     IKDIAVVRSNGIGFGTDDTLVRVLLTVVRFDAEVRAVGCIRYTEEIADVVRETLRDVA
                     EYDPSKFPPGISSMDWGIASCCRDGVPLVIIDSGTKDTEPLIRILGTTPEEVANRILI
                     ISERLTYTDI"
     gene            complement(2226..>2769)
                     /locus_tag="O0X12_01660"
     CDS             complement(2226..>2769)
                     /locus_tag="O0X12_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HJK52899.1"
                     /translation="DKALSLLYEHVKADSLRGHCIATAAVMKGLAEELSEDAGLWETI
                     GILHDIDFEEIGEDMTRHGLAGYDILKAAGVGDEIAEPIKRHNHMLFSGEYTTPVEIC
                     LQVADSVSGLVIACAYVKGGKITDVTPKTVTKKYKTASFAAGCDRNRIALGDALVPRE
                     RMYEIAIREISAVRDQLGLA"
BASE COUNT          653 a          768 c          703 g          645 t
ORIGIN      
        1 atgtcatagt cgccaagtct ctttgcagct tcacgcacca gttcccagaa gatgttgaca
       61 ccgacactga agttcgtcgt cttcacaacc ggcacaccct tgacagcatc aagcagctcg
      121 gcatcctgct cgggcgtaaa accggtcgtt ccgataacaa gagccacacc gtgggcagcc
      181 gcaatctttg cattcaccat ggttgccgca gcaaccgtga agtcgatcat cacatccggc
      241 ttctgctcct cgatgaatgc agcaaggcag gacgactcca ccaccggttt tcccagaatc
      301 tgcccaggac ggatatcaac accgccgacc agttcaaggt ccggattttc cacaaccatg
      361 ttggcgatgg ttgtgcccat gcggccaagt gcgccgcaga tcattacttt agtcatagtg
      421 ggaaagcacc tcccgaagtt tctcggtcat ttgcggattc agcgagtcca gcggcaggcg
      481 gacactgccg gaggcaataa ggccgcggat ctctgccgca cgtttgaccg gaacagggtt
      541 gacatccatg aacagtgctt tggacagctc cattagctcg tagtggattt tgcgtgcctt
      601 gtccatatcg ccgtgcacaa aggcgtcata catggcaacc atccgtttcg gctcaatgtt
      661 tgcggtaaca gagatacatc cctgaccacc gatggaaagc actggaagcg tcagcgaatc
      721 atcaccggag gtaagaacaa acggatactc gcgctccaca ccctcaagac cgtgaatgat
      781 tgccatcatc tgatcaatgt tgccgcttgc ttctttgacg gcaacgatgt tggggatggt
      841 ctttgccatc tcgatgatta agtccggcgg caggttctga ccagttctgc ccgggatgtt
      901 gtacaccacc acgggaagac cgacatctgc gacctttttg taatgcttga gaagaccgct
      961 ccggtttggt ttattgtagt aggggctgat cagaagggcg ccgtctgctc cggcaatttt
     1021 tgcggcgcgg gtcatgagaa gtgcctcctc agtgttgttg gacccggttc cggcaataac
     1081 aggaatacgg ttgtttgccg cggataccgc ttcttcaatg acacggatgt gttcggcatg
     1141 tgtcatcgtt gccgactcgc cggtcgagcc gcagggcaga agaccgtgaa cgcctccgga
     1201 gatcacatgc tcaatacagc tccggagtcc ctcaatatca agagactgtg aaggatcctc
     1261 acggaacgga gtgatgagtg caggaatcac accttcaaac atagtactta tacgtaggac
     1321 gcaccagcta catgtaactt cgcttggaca tgcacaaaaa accgaactga ccgtagcagt
     1381 atgatgcgct gatcctgaaa actatccggt acagtacaga cagaaaaaat gttttttttc
     1441 gccgggaggc ggaaccccac gggggaacgg acacatcccg cacgagtatt tgcgggggtg
     1501 ataatttacc ggcggcggtt gttggtattg accttgcggg taatgaatcc ggcaacacgg
     1561 ttgcggatga tcttactctc aatggtggtg acctcggaaa caactctctt gttgtcgtca
     1621 aagttgttgt tgaagcttgc tccgtgctgt tccatgaggg cagtgcccat agtcttgata
     1681 tagcttggct taattcccat gcgatattcc tcagatgtct gtatatgtaa gtcgttcaga
     1741 tatgataagg attctgtttg caacctcttc aggagtggtc ccaagaatcc ggatcagcgg
     1801 ctcggtatcc ttggttccgc tgtcgataat gaccagaggg acaccgtcac ggcagcagga
     1861 ggcaatcccc cagtccatac tcgagatccc cggtggaaac tttgacggat cgtactcggc
     1921 aacatcacgc agcgtttccc tgacaacgtc cgcgatctcc tcggtgtacc ggatacagcc
     1981 aaccgcgcgg acttcggcgt caaatctgac gaccgtcagc agcacgcgga caagtgtgtc
     2041 gtcagtacca aacccgattc cgttcgacct gactaccgcg atgtctttta tgtcacgggg
     2101 atttggtacg gcatacgcaa gtgccacccc gccggaagga aggagatagc gtggaatatt
     2161 catcacacga ccgtgtgcct gctgaagagc cttcagtatt acagactgct cttttgccga
     2221 cataattatg caagcccgag ctgatcccgt accgcggaaa tctcgcgaat tgcaatctca
     2281 tacatccgct cacgtggaac aagcgcatcg ccaagggcaa ttctgttccg gtcgcatccc
     2341 gcagcaaaag aggccgtttt gtacttcttt gtcaccgtct ttggcgtgac atccgtaatt
     2401 ttgcctcctt tgacgtaggc acatgcaatc accagaccgg agacactgtc ggcaacctgc
     2461 agacagatct cgaccggagt tgtatactca ccagaaaaca gcatatgatt gtggcgtttg
     2521 atcggctctg caatctcatc gccgactccg gcggccttta aaatatcata tccagcaagg
     2581 ccgtgccggg tcatatcctc gccaatttct tcgaaatcaa tgtcatgcag aataccgatc
     2641 gtctcccaca gacctgcatc ctccgaaagc tcctcggcaa gtcccttcat gacagcagcg
     2701 gtcgcaatac agtggccgcg aagcgaatcc gcctttacat gttcatacag aagggataac
     2761 gccttatcc
//
LOCUS       DALTZZ010000094         8271 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_13802,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000094 DALTZZ010000000
VERSION     DALTZZ010000094.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 8271)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8271)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8271
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13802"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="O0X12_01665"
     CDS             <1..679
                     /locus_tag="O0X12_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HJK52900.1"
                     /translation="VAGGIIGVAVGFASQNTLSNVVAGILLMFERPVNLGDNISVKGT
                     EGYVEDIGLMSTTIRTYKGVYVRVPNQAMFTSDITNYVAHVARRFDYKITIRYKDDAD
                     KAVKIIKETIEKHPYALKNPAPSVFVDDVGPNGSNLNVRIWSPSGYWWDAKTELLWKI
                     VRELRSAGIVIPFDQNVIWFGHDQTKSTDRAMYGRTASPSNPDEYLQSAAPGRDQQSP
                     ENVPQVK"
     gene            679..1161
                     /locus_tag="O0X12_01670"
     CDS             679..1161
                     /locus_tag="O0X12_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HJK52901.1"
                     /translation="MTDLNVYFNSLSDASLDVRHAAVEKIAAAGRADPGTVVPAVVSE
                     LRSGTLDTRWYLGRSLVKMGPDIIPLILEYAEVEKNMDIQKYFGAVLASFGEIAVPPL
                     VSLFSSSNPMARGMASAALERLEEKAVPALIEAALGDDPQVKLCAELTLTKLNIFEYK
                     "
     gene            1161..4595
                     /locus_tag="O0X12_01675"
     CDS             1161..4595
                     /locus_tag="O0X12_01675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HJK52902.1"
                     /translation="MAELATSPRYRAYLDHLISELDRAMEIAQTAKAKGFDPRAEVEI
                     PIASDLAGRVEALLGYKGVAARIRELEERMSREEASLKIGDAFVSREFGETTREEILD
                     HAIRASMALLTEGVVAAPTEGIGKVGIKKNDDGTEYLVIYYAGPIRSAGGTAQALSVL
                     VGDYVRRLLNLDRYKPREEEIERYVEELKQYNSIQSMQYLPKDDDIRLIIRNCPVCID
                     GEPTEKEEISGYRNLERVETNVVRGGMCLVIAEGIGLKAPKIQKNVAKMHLDGWEWLE
                     TLISGAASTSSEEEEPGIHPKDKYMRDMLAGRPPFSYPMRKGGFRLRLGRGRNTGLAT
                     CGFNPATLHVLGDYLAVGTQMKVERPGKACGVVPCDTIEGPTVRLRSGEVRRIDTLAE
                     ANKYVDAEEIEYILDVGEILISYGEFLENNHVLAPPSYCEEWWIQEGGPRHPESEAEA
                     ISFVADGAYLHPDYTWFWDDCTEDQIRFLSDKVSETGSVRDGVLYIPVDPQVKAVLEE
                     LLVPHTVEEGHYVIRTPLVLIAGLGLSYDLRKNATWNGLPPFTNGLVMAEHLSGIKMR
                     SKAGTRIGGRMGRPGKSAPRKMKPPVHVLFPIGESGGMKRSIDNAAKVCNANLSGGVF
                     SGTAVTTGQVEGMIHVETGERKCPSCGTVTYKSRCPECGTHTEAVYRCPRCNTISPPN
                     EELCPRCGAPVVCQRDSVLSLRQEYDAALAVTGLSASDIPELKGVRGLISKERVVEPL
                     EKGILRAANDVYVFRDGTVRYDMIDLPLTHFRPAEIAVSVAKLREIGYTKDMHGEELV
                     SPDQVVELNPQDILVSEDCGEYLVRVAKYMDQLLEKLYGLPAFYHAEVPEDLVGQLIL
                     GLAPHTSAGVLARLIGFTKAKAGYAHPFYHAAKRRNCDGDEDCVMLLMDGLVNFSRSF
                     LPSTRGGTMDAPLVLTTTLNPKEVDKETLNVDVMDHYPLEVYEACLTYTPPKNLEKIV
                     DHVESRVGTPGQFEGFSFTHDTRDISEGPLDTMYTNPILKGTTDKIKAELELADRIRA
                     VETDDLAERIINSHLMPDMIGNLRSFSKQSFRCPRCKTSFRRIPISGKCTKCGAVLKA
                     TMHKGNVTKYLEIAKYMAEHYQLSEYTNQRIAVTEMNIWSTFGQEEKQQMDLSDFF"
     gene            complement(4696..5985)
                     /gene="aspS"
                     /locus_tag="O0X12_01680"
     CDS             complement(4696..5985)
                     /gene="aspS"
                     /locus_tag="O0X12_01680"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HJK52903.1"
                     /translation="MRIPIKDVTPDCERAEIAGWVHEERDLGGLTFLLVRDRTGILQV
                     TIPKKKVSPEVLAAVKEVSRESVISCEGVVKATEKAPGGRELVPDTLKVISRADTPLP
                     LDVSEKVPADLDTRLDNRYLDLRKPRVNAIFLIRNAALRAINEFLWDHHFTQVQTPKI
                     VAAATEGGTELFPLAYFDKEAFLNQSPQLYKQMLMSAGFDRVFEIGPIFRAEEHNTVR
                     HLNEATSIDIEMSFADEQDAMKVLEETVSYAYRKVADTCSNELAVLGLNDFEVPATPF
                     PKITYQEAIDIATAKGEALVFGDDLTTAAEKIVGDEMGTMYFITEWPSSTRPFYTMPF
                     EDRPEVCRAFDMMHPRMELSSGAQRCHIYDLLVNQIKAKGLNPDAFAFYLNPFRYGMP
                     PHAGWGLGAERLIMTMLDLPNIREGVLFPRDRHRVSP"
     gene            complement(6150..6401)
                     /locus_tag="O0X12_01685"
     CDS             complement(6150..6401)
                     /locus_tag="O0X12_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK52904.1"
                     /translation="MTMPKMTISTPKEGAMGQTGSWRTFRPVVNREICNHCGICAMYC
                     PDAVIDPDNEEIDLVYCKGCGICANECPKKCIEMVREEH"
     gene            complement(6402..6929)
                     /locus_tag="O0X12_01690"
     CDS             complement(6402..6929)
                     /locus_tag="O0X12_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HJK52905.1"
                     /translation="MYEIRIHSRGGQGGVTAARMMAAAAVKDGKFATACPFYGAERRG
                     APIVSFVRIDDHPVRIYSQIKKPDMIIVLDPTVMETVNVLDGLKDGGAIFINTHENVQ
                     FPDKFTVHKADLTGIALSENLVVAGSPILNTPVIGALAKLGLFTPESGEAAITDTFTD
                     TRNVTVAKKAYDEVI"
     gene            complement(6930..8060)
                     /gene="porA"
                     /locus_tag="O0X12_01695"
     CDS             complement(6930..8060)
                     /gene="porA"
                     /locus_tag="O0X12_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HJK52906.1"
                     /translation="MSDKLVMSTGNKAMASAVKMAKPTVVAAYPITPQTEVIESIADY
                     VESGAMDAKYIPVESEHSAMTACIGASITGVRVFTATSSHGLLYMHEMLHWAAGARLP
                     IVMGQVNRTLAPGWNIWAEHSDALSQRDTGWLQVYVSTVQEAYDATLMAFRIAEDNRV
                     LLPVMINMDGFLLSHIMQPFEMTEPGDFIPPINLPHKIDVNNPMGYGAMSPSDIHFTF
                     RHDMEKAMKNARTVIAETEAEFAKRFGRSYAPVEEYLCDDADVVIVAMGTLGKEAEVA
                     ADLLRKEGIKAGVMRIRWLRPFPLDMKIEGKELVVIDRDYSFGFGGVIAGEIQARYPD
                     AKIARVIAGLGGQEVTYEDMAELVRTRKIGTEFWFGIPEEEQ"
     gene            complement(8057..>8271)
                     /locus_tag="O0X12_01700"
     CDS             complement(8057..>8271)
                     /locus_tag="O0X12_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate synthase subunit beta"
                     /protein_id="HJK52907.1"
                     /translation="WVLWEKEYDKFTISAPSRAAMKKPAPVIDYLKAQGRFRKVDEAT
                     AARIQAQVDKNLKKVAAEAAYSEENA"
BASE COUNT         1859 a         2059 c         2292 g         2061 t
ORIGIN      
        1 ggttgcagga gggatcattg gtgttgcagt aggttttgcc agtcagaaca ccttgtccaa
       61 tgtggtcgct ggaatcttgc tgatgtttga gcggccagtg aaccttggcg acaacatcag
      121 tgtcaaagga accgaagggt atgttgaaga catcggcctg atgtcaacaa cgatccgtac
      181 ctacaaggga gtgtacgttc gtgtcccaaa tcaggcaatg ttcacctccg acattacgaa
      241 ttacgttgct catgttgccc gcaggtttga ttataagatt accatccggt acaaagatga
      301 tgctgataaa gcggtgaaga tcatcaagga aaccatcgaa aagcatcctt atgctctgaa
      361 aaatccggct ccgtccgtgt tcgtggacga tgtggggccg aacggttcaa atctcaatgt
      421 ccgtatctgg tcgccgtcgg ggtactggtg ggatgcaaag accgaacttt tgtggaaaat
      481 tgtccgggaa cttcgttctg cggggattgt tatcccgttc gatcagaatg ttatctggtt
      541 tggacatgat caaacaaagt caacagacag ggcaatgtac ggtcgtactg cctctccatc
      601 aaatcccgat gaatatcttc agtccgcagc tcctggacgg gatcagcagt ctccggaaaa
      661 tgttcctcag gtgaagtaat gacagatctt aatgtgtatt tcaattctct cagtgatgcg
      721 tctcttgacg ttcgtcatgc cgcagttgaa aagattgccg cagcaggccg tgccgatccc
      781 gggacggttg tcccggctgt agtatccgag ctccggtccg gtacgcttga tacccgctgg
      841 tatctcggac gatcgctggt gaagatgggg ccggacatta ttcctctcat tcttgagtac
      901 gccgaggtgg agaagaatat ggatatacag aagtactttg gggcagtcct tgcttcattt
      961 ggtgagattg cagttccgcc tcttgtgtca ttattctcct cctccaatcc gatggcccgc
     1021 ggcatggcat ccgcagccct cgagaggctt gaggaaaaag cagttccggc actgattgaa
     1081 gctgctctgg gtgatgatcc gcaggtgaaa ctctgcgctg aactgacgct gacgaaactc
     1141 aacatctttg agtacaaata atggcagaac ttgcaacatc tccccggtat cgtgcatatc
     1201 tggatcattt gatcagtgaa ctcgatcggg cgatggagat tgcacagacc gcgaaggcga
     1261 agggttttga tccgcgtgca gaggtggaga tcccgatcgc aagcgatctt gcaggccgtg
     1321 tcgaggcatt gctgggttac aagggtgttg cagctcgtat ccgagaactt gaggagcgga
     1381 tgagccgtga agaggcgtct ttgaagatcg gagatgcgtt tgtgtcccgc gagtttggcg
     1441 agactacccg tgaagagatt ctggatcatg cgattcgtgc atcgatggca ctcctaaccg
     1501 agggtgtggt tgccgctccg acggaaggta tcgggaaggt tggtatcaaa aagaatgatg
     1561 acggtaccga gtatcttgtc atctactatg cagggccgat ccgatctgcg ggtggtactg
     1621 ctcaggcatt gtcggtactg gtcggtgatt atgttcggcg tctcctcaat ctcgatcgtt
     1681 ataagcctcg ggaggaggag attgagcggt atgtggagga gctaaagcag tacaacagta
     1741 ttcagagtat gcagtatctt cccaaggatg atgatatcag gctcattatc cgtaactgcc
     1801 cggtctgtat tgacggcgaa ccgactgaaa aagaggagat ctccgggtac cggaatcttg
     1861 agagggtgga aacaaacgtc gtccggggcg gtatgtgtct tgtgattgcg gaaggtatcg
     1921 gtctcaaagc tccgaagatc cagaagaacg tagctaagat gcatctcgac ggctgggagt
     1981 ggctggagac actgatctcg ggagcggcat cgacatcctc tgaggaggaa gaacctggca
     2041 ttcatccaaa ggataagtac atgcgggata tgcttgcagg ccgcccgccg ttttcgtacc
     2101 cgatgcgcaa agggggtttc cgcctgcggc ttggccgcgg gagaaacacc ggccttgcaa
     2161 cctgcggctt taatccggcc acgctccatg ttctgggtga ttaccttgcg gttggtacgc
     2221 agatgaaagt ggaacggccg ggaaaagcgt gcggtgttgt tccctgtgat acgatcgaag
     2281 ggccgacggt ccgtctcaga tcgggagagg tccggcgcat cgacacactc gcggaggcga
     2341 acaagtatgt tgacgcagag gagattgagt acatcctcga tgtcggcgaa attctgatct
     2401 catacggaga gtttctcgaa aacaatcatg tacttgctcc gccgagttac tgtgaggagt
     2461 ggtggattca ggagggcggc ccccgccatc cggagagcga ggccgaggcg atctcttttg
     2521 ttgcagatgg tgcatatctg catcccgact acacctggtt ctgggacgac tgcaccgagg
     2581 atcagatccg gttcctctcc gacaaagttt ccgagaccgg ttcggtacgg gacggtgttt
     2641 tgtatatccc tgtggatccc caggttaagg ctgttcttga agaactgctt gttccgcaca
     2701 cggttgagga agggcattat gtcatccgga caccgcttgt actgattgcc ggtcttggtc
     2761 tttcctatga cctgcggaaa aatgcaacat ggaatggcct ccctccgttc acaaacggcc
     2821 tggtaatggc agagcatctt tccggcatta agatgcgctc aaaggcgggg acccgcattg
     2881 gcggcaggat gggccgtccg ggaaagtccg ctccccgcaa aatgaaaccc ccggtacatg
     2941 tactgtttcc gattggtgag tccggaggaa tgaaacgatc cattgacaac gccgccaagg
     3001 tctgtaatgc aaatctatcg ggcggcgtgt tttcaggaac cgcggtgacc accggtcagg
     3061 ttgaaggcat gattcacgta gagaccggtg agcggaaatg tccgtcatgc ggtacagtga
     3121 cctacaaaag ccggtgtccg gagtgcggca cacatactga agctgtgtac cgctgtccgc
     3181 ggtgcaacac gatatctccg ccaaacgaag agttgtgtcc gcggtgtggt gctccggtgg
     3241 tctgccagag ggacagtgtc ttaagcctcc ggcaggagta tgacgctgca cttgcagtaa
     3301 ctggtctttc tgcaagtgat atccctgaac tgaaaggtgt ccgcggtctc atctcgaaag
     3361 agcgtgtcgt tgaaccgctg gaaaaaggaa tccttcgtgc agcaaatgat gtgtatgtgt
     3421 ttcgggacgg aactgtgcgc tatgacatga tcgatctgcc cctgacgcat ttccggcctg
     3481 cggagattgc agtttcggtt gcaaaacttc gggaaattgg ctacacaaaa gacatgcatg
     3541 gtgaggaact tgtctcaccc gaccaggtgg tggaactgaa tccgcaggat attctcgttt
     3601 ctgaggactg cggggagtat ctggtacggg tggcaaagta tatggaccag cttctggaaa
     3661 agctgtatgg tctcccggca ttttatcatg cggaagtacc cgaggatctg gtaggtcagc
     3721 ttattcttgg tcttgctccg cataccagtg ctggagttct tgcacggctg atcggtttca
     3781 ccaaagcgaa ggcggggtat gctcatccgt tctaccatgc ggcaaaacgg cggaactgtg
     3841 acggtgatga ggactgcgtg atgctgctga tggacggtct tgtaaacttc tcgcgttcct
     3901 tccttccgag tacccgtggc ggtacgatgg atgccccgct tgttctgaca acgacactca
     3961 acccaaagga ggtggataag gagacgctga acgtggatgt gatggatcat tatccacttg
     4021 aggtgtatga ggcgtgtctt acctacaccc cgccaaagaa tctggaaaaa atcgtggatc
     4081 atgtggagag cagggtcggg acgccgggac agtttgaagg attttccttt acgcacgaca
     4141 cccgtgacat ctcggaaggt ccgctggaca ccatgtacac gaatcctatt ctgaaaggaa
     4201 ccacggacaa gatcaaagcg gaactggaac ttgcggatcg aattcgtgcg gtggagacgg
     4261 atgatcttgc ggaacggatc atcaacagcc atctgatgcc ggacatgata ggaaaccttc
     4321 gttcgttttc caagcagtca ttccgctgtc cgcggtgtaa gacgagtttc cgcagaattc
     4381 caatctcggg aaaatgtaca aagtgcggtg cggttctcaa ggcaacaatg cacaaaggaa
     4441 acgtcacgaa gtatctggag attgcaaagt acatggccga acactatcag ttatcggagt
     4501 acacaaacca gcgtattgcg gtgacggaga tgaacatctg gtcaacattc gggcaggaag
     4561 aaaagcagca gatggatctg tcggacttct tctaatcaga gtttctcggt acggaatgtt
     4621 gcgtgatcag atcgtctatt tttagaattt ttttgaaaaa tgtgttgaga aaaaatatag
     4681 gaggttttat tccggttacg ggctgactct gtgccggtca cgggggaaga gtactccttc
     4741 acggatgttg gggagatcga gcatcgtcat gataagtctc tcagcaccca gtccccatcc
     4801 tgcgtgcggc ggcattccgt agcggaacgg gttgagatag aacgcgaatg catccggatt
     4861 gaggcccttt gctttgatct ggtttacgag cagatcatag atgtggcaac gctgggcacc
     4921 ggaggaaagc tccattctcg ggtgcatcat atcaaatgct ctgcagacct caggtctgtc
     4981 ctcaaacggc atggtgtaga acggtctggt tgaggacggc cactcggtga tgaagtacat
     5041 cgtgcccatc tcgtcaccga cgatcttctc tgcggcggtg gtgagatcat caccgaagac
     5101 aagggcttca ccttttgctg ttgcaatgtc gattgcttcc tgataggtga tcttggggaa
     5161 gggagttgct ggaacctcga agtcgttgag accaaggacg gcaagttcgt tgctgcacgt
     5221 atcggcaacc ttccggtatg cgtatgagac ggtctcttcg agtactttca tggcgtcctg
     5281 ttcgtcggca aaggacatct caatatcaat cgaggttgct tcgtttaagt gacggacggt
     5341 gttgtgctcc tctgcgcgga agattggacc aatctcgaag acacggtcga accctgcgct
     5401 catgagcatc tgcttgtaga gctgagggct ctggttgagg aatgcctcct tgtcgaagta
     5461 tgcaaggggg aaaagctctg ttccgccttc ggttgcggct gcgacgatct ttggtgtctg
     5521 cacctgggtg aagtggtggt cccagaggaa ctcattgatc gcacgcaggg ctgcgttacg
     5581 gattagaaag attgcgttga cgcggggctt gcgcaggtca agataacggt tgtcgaggcg
     5641 ggtgtcaaga tctgccggaa ctttttcgga gacatccagc ggcagcgggg tgtctgcgcg
     5701 ggagataacc ttgagggtat cggggacaag ttcccgtcct ccgggtgctt tttcggttgc
     5761 cttgacaact ccttcgcagg agataacaga ctctctgctt acttctttga ctgcggcgag
     5821 cacttcgggg ctgacttttt tcttggggat ggttacctgt agaattcctg ttctgtcgcg
     5881 gaccaggagg aaggtgagac cgccgagatc acgctcttcg tgaacccatc cggcgatctc
     5941 tgcccgctca cagtcggggg tgacgtcttt tattgggatg cgcataaaat ctctatgtta
     6001 tttgcggtct ggatagatta gtgtttgtcc tgcctcatgc tgttgtaaag ttacccgtct
     6061 ttctgtgata atggcatcag gggagaactc ttttcccttg atacattttc agaacacaac
     6121 tgagaaaaaa agaaaatcag aaagaagcct tagtgctcct ctcgtaccat ctcgatgcac
     6181 ttcttcgggc actcgtttgc acaaatcccg cagcccttgc agtacaccag atcgatctcc
     6241 tcattgtcgg gatcgatcac cgcatcagga cagtacatcg cacagatacc gcagtggtta
     6301 cagatttcgc ggtttaccac tgggcggaat gttctccagg aaccggtctg tcccattgcc
     6361 ccttcctttg gagtactgat tgtcatcttc ggcattgtca tttagatcac ctcatcgtat
     6421 gccttctttg caaccgtcac attccgggta tccgtaaatg tatccgtgat agctgcctct
     6481 cccgactcag gcgtgaacag accaagtttt gcaagagctc cgatcaccgg cgtgttcaga
     6541 atcgggcttc cggcaacaac aagattctct gaaagcgcga ttcctgtcag atccgctttg
     6601 tgcactgtga acttgtcagg gaactgcacg ttctcatgcg tgttgatgaa gattgctcct
     6661 ccgtctttga gaccgtccag tacgtttacg gtctccatca cggttggatc gagaacaatg
     6721 atcatatccg gctttttaat ctgtgagtag atccggaccg ggtgatcatc aatccgcacg
     6781 aaggagacga tcggcgcacc acggcgttct gcaccgtaga acgggcatgc tgttgcaaat
     6841 tttccatctt taactgctgc ggctgccatc atgcgtgcag cggttacacc accctgtccg
     6901 ccgcgtgaat ggatacgaat ctcgtacatt tactgttcct cctcggggat gccaaaccag
     6961 aactcggttc caatcttgcg tgtgcggaca agttctgcca tatcctcgta tgttacctcc
     7021 tgaccgccaa gaccggcaat gacccgtgca atctttgcat ccggatatct tgcctggatc
     7081 tctcctgcga tcacaccgcc gaatccgaac gagtaatcac gatcgatcac aaccagttcc
     7141 tttccttcga tcttcatgtc aagcgggaac ggccggagcc agcggatgcg catcacacct
     7201 gctttgatac cctctttgcg gagaagatcc gctgcaacct cagcctcttt tccaagagtt
     7261 cccattgcaa cgatcacaac atctgcatca tcgcagagat actcttcgac cggtgcatag
     7321 cttctgccaa accgctttgc aaactctgcc tcagtttcgg caatcacggt tcgggcgttc
     7381 ttcattgcct tctccatatc atgccggaag gtgaagtgaa tatcagacgg cgacattgcc
     7441 ccgtatccca tcgggttgtt cacatcaatc ttgtgcggca gattgattgg cgggatgaaa
     7501 tctcccggct ctgtcatctc gaacggctgc atgatgtgag agagcaggaa tccgtccatg
     7561 ttgatcatca ccggcagcag cactctgtta tcctcggcaa ttctgaaggc catcagtgtt
     7621 gcatcgtatg cttcctggac cgttgaaaca tacacctgta gccatccggt atcacgctgg
     7681 gagagagcat ccgagtgttc agcccagatg ttccatccag gcgcaagtgt acggtttacc
     7741 tgtcccatca caatcggaag ccgtgcacct gctgcccagt gcagcatctc atgcatgtac
     7801 agaagaccgt gcgaacttgt tgccgtaaac acacggacgc ctgtgattga tgcaccgata
     7861 caggcggtca ttgcagagtg ttccgattcg accgggatat acttggcatc cattgcaccg
     7921 ctctcgacat aatctgcaat cgactcaata acctcggtct gtggtgtgat cggatatgcc
     7981 gcaaccacgg tcggctttgc catcttcact gcagatgcca ttgccttgtt accggttgac
     8041 attaccagtt tgtcgctcat gcgttctcct ccgagtatgc cgcttctgct gcaactttct
     8101 tcagattctt gtcaacctgt gcctgaatcc gtgcagcggt tgcctcatcc accttgcgga
     8161 atcttccctg ggctttcagg tagtcaatca ccggtgcagg ttttttcatc gctgcacgcg
     8221 acggggcact gatggtaaat ttgtcgtact ccttttccca gagcacccag a
//
LOCUS       DALTZZ010000095         1993 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_13935,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000095 DALTZZ010000000
VERSION     DALTZZ010000095.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13935"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="O0X12_01705"
     CDS             complement(<1..176)
                     /locus_tag="O0X12_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor 6"
                     /protein_id="HJK52908.1"
                     /translation="MDHTLSLNGDPNIGVFARVFEDIAFVPLDSPEEFRTKIAEALDV
                     EVIPTFIQGSSVIGL"
     gene            complement(177..440)
                     /locus_tag="O0X12_01710"
     CDS             complement(177..440)
                     /locus_tag="O0X12_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HJK52909.1"
                     /translation="MVEAQKEQVYVIPLRDVKRVPCYKRANAAIKDIRSYLEHHMKSD
                     DVKLDKSINEAVWARGSQKPPRRIRVRAMKFEDGQVQAELAEE"
     gene            complement(445..597)
                     /locus_tag="O0X12_01715"
     CDS             complement(445..597)
                     /locus_tag="O0X12_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HJK52910.1"
                     /translation="MSKLTKARKIRLAKATQQNRRVPAWVMIKTKRNVVSHPCRRNWR
                     RSTLKV"
     gene            complement(600..1193)
                     /locus_tag="O0X12_01720"
     CDS             complement(600..1193)
                     /locus_tag="O0X12_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha hydrolase"
                     /protein_id="HJK52911.1"
                     /translation="MKAGVLYSGGKDSTLAAVLLARDYEVELITFVFDPNHAVPSIEA
                     AAKATGFPWKKQVFAPGFLDEVVNMIVEDGHPANAINEIHRRSLCALAQEYEVVADGT
                     RRDDRVPMRTQSEVQSLEMKYGVSYVRPLLGIGKREIIRLCERSFEIAYGETGTIPNG
                     DFESEIRAEMVRRGIDYVPLFPKNHEQSLIIRKKNGD"
     gene            complement(1190..1522)
                     /locus_tag="O0X12_01725"
     CDS             complement(1190..1522)
                     /locus_tag="O0X12_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HJK52912.1"
                     /translation="MGDDELAELRRQRMMQLQQQQMAEQEQAQRQQQMQAQIQSVLMQ
                     VMEPEARERLNTIKLTKPEFAAAVEQQIVALAQSGRLRQKITDDQLKQLLSQIVPQKK
                     EFNIRRVG"
     gene            complement(1556..>1993)
                     /locus_tag="O0X12_01730"
     CDS             complement(1556..>1993)
                     /locus_tag="O0X12_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HJK52913.1"
                     /translation="FDIPANALIAKVAEELKNESEIKAPEWSEYVKTGVHKQMPPENP
                     DWWYIRSAAVLRRIYVDGPIGVERMRSVYGGMQDRGSKPSHFRKGSGSIARKVMQQLE
                     AAGYIEKVTGGRSVSAKGRKFLDNIAHSLKEEAVKEAPGLAKY"
BASE COUNT          411 a          543 c          495 g          544 t
ORIGIN      
        1 agaccgatca cggatgatcc ctgaataaag gtaggaatga cctctacatc aagagcttcc
       61 gctatctttg ttctgaactc ctccggagag tcgagcggaa caaatgctat atcttcaaag
      121 acacgggcaa agacaccgat gttcgggtct ccattcagtg acagggtgtg gtccatttat
      181 tcctcagcaa gttctgcctg gacctgtccg tcttcgaact tcattgcacg aacacgaatg
      241 cgtcttggcg gtttctggga tccacgtgcc cagactgctt cgttaatgct cttgtcaagc
      301 ttaacgtcat cacttttcat gtgatgttca aggtagctgc ggatgtcttt gattgcagcg
      361 tttgcacgct tgtagcacgg gacacgcttg acatcacgga gcggaataac atatacctgt
      421 tccttttgtg cttctaccat tttcttacac cttcagagtg ctgcgtctcc agttgcgtct
      481 gcacgggtga gacacaacgt tacgcttggt tttgatcatg acccaggccg gaacacggcg
      541 gttctgctgg gttgcctttg caagacgaat cttacgagct ttagtaagtt tgctcatggt
      601 taatcaccat tcttttttcg aatgatcaac gactgttcat ggtttttcgg gaagagcggc
      661 acatagtcaa ttccccggcg aaccatttca gccctgatct cactttcgaa atcaccgttt
      721 ggaatcgtcc cggtttcgcc gtaggcgatc tcaaaggacc gttcacacag acggataatt
      781 tctctttttc caatgccaag cagaggccgc acatagctga ccccgtactt catctcaagg
      841 ctctgtactt cggactgtgt ccgcatcggg acccggtcat cacgacgggt tccgtcggcg
      901 accacctcat attcctgtgc aagtgcacag agagaccgtc ggtggatttc attgatggca
      961 tttgcaggat gcccgtcctc aacaatcatg ttgacgactt catcaaggaa acctggcgca
     1021 aagacctgtt tcttccaggg gaaaccggtt gcctttgccg ccgcctcaat ggaagggaca
     1081 gcatggtttg gatcgaatac gaaggttatc agctccacct catagtcccg cgcaagcaga
     1141 actgcggcaa gcgtactgtc tttgccgccg ctgtagagca cacccgcttt catccaaccc
     1201 tgcggatatt gaattccttt ttctgcggta caatttgcga aagcaactgc ttgagttggt
     1261 catcagtaat tttctgacgc aggcgtcctg actgggcaag agcgacaatc tgctgctcga
     1321 ctgctgcggc aaactcaggt ttggtcagct tgatagtgtt cagccgttcg cgggcttcag
     1381 gttccattac ctgcataaga acagactgga tctgtgcctg catctgctgc tggcgctggg
     1441 cctgttcctg ttcagccatc tgctgctgct ggagctgcat catgcgctgc cggcgaagtt
     1501 ctgcgagttc atcgtctccc atttctggtc accccattct gttgtcagat attctttagt
     1561 actttgcaag acccggagct tccttgacag cctcttcctt taaggagtgg gcaatgttgt
     1621 cgaggaactt gcggcctttt gcggagacac tacggccgcc ggtaactttc tcaatgtagc
     1681 cggctgcttc gagctgctgc ataacttttc gtgcaatgct gccgcttccc tttctgaagt
     1741 gggacggctt agagccgcgg tcctgcatac caccatagac cgagcgcatg cgctcgactc
     1801 caatcgggcc gtcaacatag atacgacgca gcactgctgc ggaacggatg taccaccagt
     1861 ctggattctc tggcggcatc tgcttgtgta ctcccgtttt tacgtactca gaccactcgg
     1921 gtgccttgat ctcggactcg ttcttgagtt cctcggcaac ctttgcgatg agtgcattgg
     1981 ctgggatgtc aaa
//
LOCUS       DALTZZ010000096         3334 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_13966,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000096 DALTZZ010000000
VERSION     DALTZZ010000096.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13966"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="O0X12_01735"
     CDS             complement(<1..233)
                     /locus_tag="O0X12_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52914.1"
                     /translation="MYQFMDPLTVLFAYGATWVDTLNYTLGAAVIALAFVYFTRNLSF
                     VQRVAAPAIVGVLVSVLGTGVVISASALPQLGYP"
     gene            complement(309..1970)
                     /gene="thsA"
                     /locus_tag="O0X12_01740"
     CDS             complement(309..1970)
                     /gene="thsA"
                     /locus_tag="O0X12_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HJK52915.1"
                     /translation="MSAQLGGQPILILKEGGSRTRGRDAQSMNIAAAKAVAGAVRTTL
                     GPKGMDKMLVDTIGDVVITNDGVTILKEMDIEHPAAKMMVEVAKTQDDEVGDGTTSAV
                     VIAGELLKKSEELLEQDVHPTVITLGYRQAAEKAQELLQTIAVDVKAKDKEILSKIAG
                     TAMTGKNAEASKEKLCDLIVRAITLVADADGTVDTENVKVEKRVGGAIEDSEIVEGMI
                     IDKERVHPGMPKTVKAAKILLLNAAVEYKKTEVDAEISITSPDQLQLFLDEEERMIKS
                     IVEKIKASGANVLFCQKGIDDIAQHYLSKAGILAVRRVKKSDMEKLARATGGALISSI
                     DAISADELGVAGLVEERKVGGEEMIFVEKCKNPKAVSIIIKGGTEHVVDELGRALEDA
                     LRVVGVVVEDKKIVAGGGAPEVELSLRLREYAATQGGRIQLAIEAFASALEVIPRTLA
                     ENAGLDPIDKLVELRAAHEKGEKTYGLDVFEGKPVDMLAAGVVEPLRVKTQAIASAAE
                     AAVMILRIDDVIASAKSAGPSPDEMAAMGGAGGMGGMPGGMPGMM"
     gene            complement(2290..3237)
                     /locus_tag="O0X12_01745"
     CDS             complement(2290..3237)
                     /locus_tag="O0X12_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HJK52916.1"
                     /translation="MSNDRLLQNVVSILIMAGYNVSERCEIRPRSFDLMTSDGKHLLV
                     IKVVSQIDSVNEDIAWDLDKIARHLGAVPLIIGERARDAPLERGAIYLRYGINAVSSA
                     TLYDYLAEGELPLVYASPGGLYVNIDADRLRELREEHSMSLGDLAHALGVSRRTISKY
                     EGGMGTTLDVAMRLEELFNDDIVMPIDLLSYTPAAEERSPASLPSGNSTDADPHQLPA
                     DRLRSIGISVQELRRAPFHAFAVYEDETILTCYGTAQKTVRRAELVGNISQISGTHSL
                     CVVSDYRKEKKIGKTLVIGEERLKHVSDGSDLLEMVTEK"
BASE COUNT          776 a          891 c          859 g          808 t
ORIGIN      
        1 ggatacccaa gctgcggcag ggcggatgca gagattacaa caccggttcc aagaaccgac
       61 acaaggaccc cgacaatcgc aggggcagca acccgctgaa caaaggaaag attccgggta
      121 aagtacacaa atgccagggc gatcaccgcc gcaccaagtg tgtagttgag agtatccacc
      181 caagtggcac catatgcgaa aagaacggtc agcggatcca taaactggta catgtgtttg
      241 ctttgggaga atatagcgtt tcagggagaa agagaacgtt tcaaaaaaga gattgatatt
      301 ggggagattt acatcatacc gggcatgccg ccgggcatac cgcccatgcc gcctgcaccg
      361 cccattgctg ccatctcgtc cggagacggg cctgcggact ttgcggatgc aatcacatca
      421 tcgattctga gaatcatgac tgctgcctct gctgcggatg caattgcctg tgtctttaca
      481 cggagcggct caacaacgcc tgctgcaagc atgtcaaccg gcttaccctc gaagacgtca
      541 agaccgtagg tcttctcgcc tttctcatgg gctgcacgaa gctcgacaag tttgtcgatt
      601 gggtcaagac ctgcattctc tgcaagagtt ctcgggatga cctcaagtgc ggatgcgaat
      661 gcctcgattg cgagctggat acgtccgccc tgggttgctg cgtactcacg aagtctgagg
      721 gagagctcga cttccggtgc tcctccgcct gcaacaatct tcttgtcttc aacaacaaca
      781 ccgacaacac ggagtgcgtc ctcaagtgca cggccaagct cgtcaaccac gtgctcggtt
      841 ccgcctttga tgatgatgga gactgccttc gggttcttgc acttctcaac gaagatcatc
      901 tcttcgccgc cgaccttgcg ctcttcgaca agaccagcga caccaagctc atctgcgctg
      961 attgcgtcaa tggaggagat cagtgcgccg ccggttgcac gtgccagctt ctccatgtcg
     1021 gacttcttga cacggcggac tgcgaggatg cctgccttgg agaggtagtg ctgagcaatg
     1081 tcgtcgatac ccttctgaca gaagagaacg tttgcaccgg atgccttgat cttctcgacg
     1141 atgctcttaa tcatgcgctc ttcctcatca aggaacagct ggagctggtc ggggctggtg
     1201 atgctgatct ctgcgtcaac ttcggtcttc ttgtactcga cagctgcgtt caggaggaga
     1261 atctttgcag ctttaacggt cttcggcata ccggggtgga cacgttcttt gtcgatgatc
     1321 ataccttcga caatctcgga gtcctcgatt gcgccaccga cacgcttctc gactttgacg
     1381 ttctccgtgt caacggttcc gtctgcgtca gcaacgaggg tgattgcgcg gacgattaag
     1441 tcgcagagct tctctttgga tgcttcggcg ttcttgccgg tcattgcagt acctgcaatc
     1501 ttggagagga tttccttgtc ctttgccttt acatcgactg caatggtctg gagaagttcc
     1561 tgtgccttct ctgctgcctg gcggtatcca agggtgataa cagtcgggtg gacatcctgc
     1621 tcgagaagtt cttcggattt cttcaggagt tcgcctgcga taacaacggc agaggtggtt
     1681 ccgtctccga cttcgtcgtc ctgggtcttt gcgacctcaa ccatcatctt tgctgccggg
     1741 tgctcaatat ccatctcttt gaggatggta acgccatcgt tggtgatgac aacatcgccg
     1801 atggtgtcga cgagcatctt gtccatgccc ttcggaccaa gggtagttct gactgcacct
     1861 gcaactgcct ttgctgcagc aatgttcatg ctctgggcat cccgtccacg ggtacgggaa
     1921 ccgccttctt taagaatcag aataggctgt ccgccaagtt gtgctgacat gatttctatc
     1981 tccaaatact ctgtggcata cagagtaatg tatgcagatt aggttgcttt gttgttctat
     2041 ataaatttaa ttgtattgtg aatccatcgg gacaaaaatg taagaaaatt taccaagccc
     2101 agcacaaccc cgcaggggcg cgcctgttga gttaggagat gtcttgttta tcggtgatga
     2161 atgatctcct actgactgct tttctggatg gatgacagat gaaatctgcg ggattcaata
     2221 ttatcagtac gggatcaatc agacggcgca ttaacaaaca gcggcccaaa aaatcagagg
     2281 aataaaaaat tatttttctg tcaccatctc cagcagatcc gaaccatcag agacatgctt
     2341 cagccgctcc tccccgataa caagcgtctt gccaatcttc ttttccttac gatagtcgga
     2401 taccacacat aatgagtgtg ttccagaaat ctgcgagatg ttaccaacca gctcggcacg
     2461 acggaccgtc ttttgtgcag taccgtaaca ggtgagaatc gtctcatcct catacacagc
     2521 aaacgcatgg aacggagcgc ggcgaagttc ctgtacgctg ataccgatcg atctcaggcg
     2581 gtcagcggga agctgatgcg gatcagcatc agtactattg ccggatggga gagatgccgg
     2641 ggatcgttcc tctgctgcgg gcgtgtagct gagaagatcg atcggcatca caatatcatc
     2701 attgaacagc tcctcaagcc gcattgcaac atcaagcgtc gtacccatac ccccctcata
     2761 cttgctgata gtacggcggg agaccccgag tgcatgagca agatcaccga gcgacatgga
     2821 atgttcctcg cgcagctcac gaagccggtc agcatcgatg ttcacgtaga gaccacccgg
     2881 cgaggcatag acgagcggca actctccttc cgcaagatag tcatacagtg ttgccgagga
     2941 cacggcattg attccgtagc gcagataaat tgccccgcgc tccagtgggg catcgcgtgc
     3001 ccgttcgccg ataatcaacg gaacagcacc aagatgccgg gcaattttgt ccaggtccca
     3061 ggcaatgtct tcgtttacac tgtctatctg ggacacgacc ttgatgacga gcagatgttt
     3121 cccatcagat gtcatcagat cgaaactccg cggacgtatc tcgcagcgtt ccgatacgtt
     3181 gtaacctgcc atgatcagga tgctgaccac gttctgaagc agccggtcgt tggacatgat
     3241 cgtataatcg taatagtaat gcagttcaat aaaatagtat cgatagtatg ttccttggta
     3301 tggacgacac ggactcccca gacgggatgt gtac
//
LOCUS       DALTZZ010000097         1664 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_13974,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000097 DALTZZ010000000
VERSION     DALTZZ010000097.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13974"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="O0X12_01750"
     CDS             complement(<1..424)
                     /locus_tag="O0X12_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HJK52917.1"
                     /translation="MAQYTCGVCGYTYDEAAGVLFSELSDSWLCPVCSAGKQQFTLTS
                     SPAKETGYTGTSVMDEIHYMAETGRSITEPMETLMPVPSFDEILLLGAQLSQLPLDDD
                     APVNVRTVIGKHAKQPMVLETPVFVSHMSFGALSREAKI"
     gene            complement(446..988)
                     /locus_tag="O0X12_01755"
     CDS             complement(446..988)
                     /locus_tag="O0X12_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HJK52918.1"
                     /translation="MANLKGTKTEANLQAAFAGESQARNKYNYYASKARKDGYEQIAE
                     LFEETANNEKEHAKIWFKLLHGGAVPDTLTNLADAAAGENYEWTDMYAGFAKEARAEG
                     FEDIAKLFEMVADIEKHHEERYLKLLANINEGIVFSRDGDMIWQCGNCGHLVIGKSAP
                     EVCPVCDHPKAYFRIEARNY"
     gene            1156..>1664
                     /locus_tag="O0X12_01760"
     CDS             1156..>1664
                     /locus_tag="O0X12_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJK52919.1"
                     /translation="MSELMQKIERATSSTFFDQTGRNEGSGRCCKIMRILLEGTCSFD
                     CAYCGVCTKKNGISFTPQELARGFLSLHNEGRVGGLLLSTGIPRGDVDLGMEKLIETA
                     RLIRSSGYRGYLHLKVLPGAARSDITELAKYATRLSINLEAPSASYLSELATVKNYRS
                     DLLTRHQWLA"
BASE COUNT          334 a          429 c          473 g          428 t
ORIGIN      
        1 caatctttgc ctcccgggag agggcaccaa agctcatgtg cgaaacaaac accggggttt
       61 ccagaaccat cggctgcttt gcatgctttc cgatcaccgt ccgcacattc accggtgcat
      121 catcgtccag cggcagctgg gaaagctggg cgccgagcag cagaatctcg tcaaatgacg
      181 gtaccggcat cagtgtctcc atgggctcgg taatgcttct tccggtctct gccatataat
      241 gaatctcatc cattacgctg gttccggtgt atccagtctc tttggcggga gaggatgtca
      301 gagtaaactg ctgttttccc gcggaacaga cgggacagag ccaggagtcg gaaagttctg
      361 aaaacaagac gcctgctgct tcatcatagg tatagccgca gaccccgcag gtgtactgtg
      421 ccataataaa atctccaaaa aaaagttagt agttccgggc ttcgatccgg aagtatgcct
      481 tcgggtgatc gcagaccggg cagacctcgg gagcggattt gccgatgacc aggtggccgc
      541 agttgccgca ctgccagatc atatcaccgt cacgggagaa gacgatgccc tcattgatgt
      601 ttgccaggag tttcaggtac cgctcttcgt ggtgcttctc gatgtctgca accatctcaa
      661 agagtttggc gatgtcctca aagccttctg cacgtgcttc ctttgcaaag cctgcataca
      721 tgtcggtcca ctcgtagttt tcgcctgccg cagcgtcggc aaggttggtc agcgtgtccg
      781 gaactgcacc gccgtgcaga agtttgaacc agatctttgc gtgttccttc tcgttgtttg
      841 cggtctcctc aaacagctcg gcaatctgct catagccgtc tttgcgtgcc ttggatgcgt
      901 agtagttgta cttgtttctt gcctgggact cgccggcaaa tgcggcctgg agatttgctt
      961 cggtcttggt tcctttcaga tttgccatag tgatacaacc tcggaaatga ttggtatccc
     1021 ttatgttagg gagtgcgtgg taatgaatgt gacgagtgtc cggagagccg ggtcttctgc
     1081 ggtgtttttg cgggagggca ttttcactcc gcacggtagg gggtttaagt agtgtcctgc
     1141 cgacagtatg gtgtaatgtc ggagttgatg cagaagatcg agcgggcgac aagctctacg
     1201 ttctttgatc agaccggccg caacgagggt tcgggacggt gctgcaagat catgcggata
     1261 ctgcttgagg gtacctgttc ctttgactgt gcttactgcg gtgtctgtac gaaaaagaac
     1321 ggtatcagtt tcacacccca ggagcttgcc cgaggctttc tttccctgca caatgagggg
     1381 agggtcggcg gtcttctgct tagtaccggt attccccgcg gcgatgtgga tcttggtatg
     1441 gaaaagctga tcgagactgc caggctgatt cgttcttccg ggtaccgcgg gtatctgcat
     1501 ctgaaggtgc ttccgggcgc cgcccgatcc gacattactg aacttgcgaa gtatgcaacc
     1561 cgtctgagta tcaatctgga agctccgagt gcgtcgtatc tttcagagct tgccacggtg
     1621 aagaactacc ggtctgatct gctgacccgt catcagtggc ttgc
//
LOCUS       DALTZZ010000098         9196 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_14086,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000098 DALTZZ010000000
VERSION     DALTZZ010000098.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 9196)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9196)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9196
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14086"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..2249)
                     /locus_tag="O0X12_01765"
     CDS             complement(<1..2249)
                     /locus_tag="O0X12_01765"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HJK52920.1"
                     /translation="MVFRVFVEKKPALANESHALLRELSTLPGVQGVSSLRIINRYDV
                     DNIDADLFAYAKSTVFSEPQIDIVTDTPALAEDERMFAVEYLPGQFDQRADSAAQCIQ
                     LISQKERPIVRTARVYVLGGNISDEVLDRVKKYVINPIESREADLAMPTTLVMSCAAP
                     AKVAVLEGFRELDAAGLADFSARYGLAMDTADLAFCQKYFLSENRDPTITEIRMIDTY
                     WSDHCRHTTFLTTIDSVRTESPLLQKAFDDYLAVRQSIGRTKPVNLMDIGTIAVKYLR
                     SQGKLDKLDESEEVNACTVKITVSVDGKDEDWLLLFKNETHNHPTEIEPFGGAATCIG
                     GAIRDPLSGRAYVYAAMRLTGAGNPLAPISDTLPGKLPQKKIVTTAAAGYSSYGNQIG
                     LATGMVDELYHPGYIAKRMEVGAVIGAAPANNVRREVPAAGDVVILLGGRTGRDGCGG
                     ATGSSKSHTAASLESCGAEVQKGNAPEERKIQRLFRNHDAAVLIKRCNDFGAGGVSVA
                     IGELADGVLINLNAVPKKYDGLDGTELAISESQERMAVVVAAADAQKFISLAHAENLE
                     ATIVAEVTAEPRLVMVWNGDKIVDISREFLASNGVEKHTDVEMADPQPFSREISGDFA
                     ENYRNLAEDLNVCSKRGLSERFDSTIGAGAVLMPFGGKYQQTPVQAMVTKISVEFGDT
                     DDCSLMAWGCNPQILEMSPYHGAYLAVVESVSKLVATGAEFSDVYLSFQEYFEKLGSD
                     AQRWGKPAAA"
     gene            complement(2364..2726)
                     /locus_tag="O0X12_01770"
     CDS             complement(2364..2726)
                     /locus_tag="O0X12_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HJK52921.1"
                     /translation="MPENPSECPKGRCGRPRVKRCIDLDGASGCYAPICPHRQETVTS
                     VVVFPEEIAVLKLVDLDNLSQEEAAGMLGVSRKTAWRDLHEARRKIADALVNGKAIRV
                     SCCPHENMCSSLSPDREK"
     gene            2900..4564
                     /gene="atwA"
                     /locus_tag="O0X12_01775"
     CDS             2900..4564
                     /gene="atwA"
                     /locus_tag="O0X12_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HJK52922.1"
                     /translation="MTTPFITIENLCIDFPADADNPAGEQVRILNDINLEIAEGEIVG
                     VIGRSGCGKTVLIHLLRGIEQPPTAGRIIYHISRCPACGRIEFRSSAGKKCPACGAVL
                     EAQDVDFWAPENAAIKTKIMARTSLMFQRTFALYGDDRVIENVLRALDDIDYPAEKAI
                     NRAADLIDEVRLTHRMMHVARDLSGGEKQRVVLARQLAREPLFLCADEPTGTLDPKTA
                     TIVHGLLKHAAKSTNMGMVITSHFSQVLEDVCDRAVLLDDGVITKIGEPAEIVAEFMK
                     DCEDDLEYSDQEIGNPIVRADKLYKKFIAVDRGVIKAVDNITFEINEREIFGVIGVSG
                     GGKTTLSKMIAGLYEPTAGKLDVRIGDEWIDMTKPGYQFRGRAKQYIGLLHQEYDLYP
                     HRTIIDNLTDAIGLEFPKELAVRKAIITLRMAGFTDKKAKDVLNRYPSSLSAGEKHRV
                     ALAQVLIREPRIILLDEPTGTMDPITKVDVKHSIIHAREEMDETFIIVSHDMEFVRDV
                     CDRCMFIRGGKIIDIGPTHEVLAKLSEQEISTMAKAVAEERAGAEQ"
     gene            complement(4595..5434)
                     /locus_tag="O0X12_01780"
     CDS             complement(4595..5434)
                     /locus_tag="O0X12_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52923.1"
                     /translation="MKSVAEMLELDSLRFLEPDYASLVNNGEPGILICEAEPTPGGAR
                     MLIDEEENPVLFAVRAGDEWVGTSWNFRSPMEQEFALFTAADGDLYQEPRAAIDDAVR
                     AHFSQILREEIAPAREDLPPDRIEKVRDLLHEVWGASAPGLCLDACAGSGIGSMIIRE
                     MGGRPIAYDNDPALLALGLASGRLAPEDTICIDGTVASAYLPDAERGLGIMFGQMYVY
                     TQNLWQPIVEELAGITAETLITVATEEEAHWVREWAKGVDRDLEIWENDRDPIYDRWV
                     CFG"
     gene            complement(5431..6456)
                     /locus_tag="O0X12_01785"
     CDS             complement(5431..6456)
                     /locus_tag="O0X12_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HJK52924.1"
                     /translation="MAEGKKRVIVAEPIPENVLIEAEEIPVEEVEQIRDVPPSPAAET
                     EELPAEEPQRSEDASAVATMATAELFKKCILPDKTELQERILKTKNDILVGDHCDIGF
                     GLYGNDVVVCEFCNLTGDVVAEGDLRIDNFCEINGTVICNGDAYLGEGVKVHGKLTVG
                     GNLDIGDNVTIDKEFKAFGDISIRNPMPVILYLLLYVMTMLHLDNEEEVEKTVNAIIS
                     EANSEPLVLPPRTTLDLRYFSVSTPMEVGANCRLHGNIKARAITLKNDVTLFGGIHAT
                     RRVKIGERTAVHGDVIGQNIRIERGADILGDVSGDKVWLHEDALVSGIIRSPGGLTIG
                     RFEGKKE"
     gene            complement(6487..7710)
                     /locus_tag="O0X12_01790"
     CDS             complement(6487..7710)
                     /locus_tag="O0X12_01790"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HJK52925.1"
                     /translation="MTASKILLLIIDGVGDRPCDCLGGKTPLQAANISNLDELARTGI
                     CGIMDPVAPGVRAGSDTSHLSLLGYPPQKYYTGRGPLEAAGCGIRMEPGMIGFRANYA
                     TLDDAGNVIDRRAGRISDTTEISAAIRDGVDLSKYGVTIEFEPGTGHRAALALKGAGL
                     SAAVSSNDPKTTGVPPKMIHPEGEDTPEAKFTAEVCNEFCRQAEEILKNHPVNLARKA
                     AGQNPANVVLIRGAGAMGVYEPFEERHELSGSVVAAAALIAGIGSSVGLRRIPVKPDT
                     PLAEQVSLVKREFETQDFVLFNIKNADEYGHDGKAMEKKEFLEKIDKELRPLMEMPEL
                     LIVVCGDHSTPCTIKEHSADPVPLIIHGNGARIDQVSSYDEISCAAGALCRIRGGDLM
                     PILLDLIDKTHKYGA"
     gene            complement(7778..7852)
                     /locus_tag="O0X12_01795"
     tRNA            complement(7778..7852)
                     /locus_tag="O0X12_01795"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7815..7817),aa:Met,seq:cat)
     gene            7949..8359
                     /gene="trxA"
                     /locus_tag="O0X12_01800"
     CDS             7949..8359
                     /gene="trxA"
                     /locus_tag="O0X12_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HJK52926.1"
                     /translation="MEDDELEQLRKKRFEQLAAKIPPPEGWPVIELTEYNFNEVTLGR
                     PKVVIDFWAEWCGPCRYFSPIFEEMSVEYPEVQFCKCDTDRNHGIAKQLGITSIPRVL
                     YVINGTAVRLHVGAMSAEKFREELNAVFRSNDTV"
     gene            8346..>9196
                     /locus_tag="O0X12_01805"
     CDS             8346..>9196
                     /locus_tag="O0X12_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJK52927.1"
                     /translation="MTLFESFHPSLQEVLLSGLGWSGLRPVQEETYRTVAGGADVVVL
                     APTAGGKTESAFIPVIDALLKTGSRGLGAIYLSPLKALINDQQDRILLMCGRAGLTVA
                     SQHGDVAARDRWKFSPEADTPNLLLTTPESLEVLLGDPNSRSAFSSLRFVVIDEIHAF
                     METDRGVHLRCLLDRLERITKNTVVQRIGLSATVGNPEELLAWMSGPNRKKQLVQIPS
                     PATPKQFSFVVEEEFTAQIRAVADAVRGKKALVFVDSRSFAERLMAPLSDLVPGVYLH
                     HSSVSAE"
BASE COUNT         2293 a         2356 c         2383 g         2164 t
ORIGIN      
        1 gcagcagcgg gttttcccca acgctgtgca tcagaaccga gcttttcgaa gtactcctgg
       61 aaggaaagat acacatccga aaactcggca ccggtagcaa ccagcttgga tacgctttca
      121 actactgcaa gatatgcccc gtgataagga ctcatctcca gaatctgtgg gttgcatccc
      181 catgccatca gagaacagtc atcggtatca ccaaactcaa cggaaatctt ggtgaccatt
      241 gcctgcaccg gtgtctgctg gtattttccg ccgaacggca tcagcacggc tccggcacca
      301 atcgttgaat caaaccgctc ggagagtccg cgcttggaac agacattcag atcctctgca
      361 agattccggt agttctccgc gaagtccccg gaaatctcgc gggaaaacgg ctgcggatct
      421 gccatctcca catcagtgtg cttttcaaca ccgtttgatg caaggaactc acgtgagatg
      481 tcgacaatct tgtccccgtt ccagaccatc accagacgcg gttcagcggt gacctctgcc
      541 acaatcgtag cctcaagatt ttccgcatgt gcaagcgaga taaacttctg agcatcagca
      601 gccgcaacaa caacagccat acgctcctgc gactcactaa ttgcaagctc cgttccatcc
      661 agaccatcat actttttggg gaccgcatta agattgatca agacaccgtc ggcaagttct
      721 ccaatagcga cagaaacccc accagcacca aaatcattac agcgtttgat caaaactgcg
      781 gcatcgtgat tacggaacag ccgctggatc ttccgctctt caggtgcatt tcctttctgc
      841 acctcagcac cgcaggactc aagggacgcc gcggtgtgag acttggatga acctgttgct
      901 ccgccgcacc cgtcacgtcc ggttctacct ccaaggagaa tcaccacatc tcctgcggca
      961 ggaacctcgc ggcgcacatt gttggctggt gccgcaccaa taacggcacc aacctccatt
     1021 cgttttgcga tgtaaccagg atgataaagt tcatccacca taccggtggc taggcctatc
     1081 tggtttccat aggaactgta accggcggca gcagtggtaa cgattttctt ctgaggaagt
     1141 tttccgggaa gtgtgtcact gattggggca agcgggtttc cggcaccggt cagccgcatc
     1201 gcagcataca cgtaggccct tccagagagc ggatcacgaa ttgcaccgcc gatacaggtt
     1261 gctgcccctc caaacggctc gatctcagta ggatggttgt gtgtctcgtt tttgaacaaa
     1321 agcagccagt cctcatcttt cccatcaaca gagacagtaa tcttcacagt acaggcgttg
     1381 acctcctcgg actcatcgag cttgtcaagc ttaccctggc tccgcagata tttgacagca
     1441 attgttccaa tgtccatcag gttgacaggt tttgttctgc cgatagactg ccggactgca
     1501 agataatcgt caaaggcttt ctgcaggagg ggactttcgg tccggacact atcaatagtg
     1561 gtaaggaacg ttgtgtggcg gcaatgatcc gaccagtagg tatcaatcat cctaatctca
     1621 gtaatagtcg gatcacgatt ctcactaagg aagtacttct gacagaaggc gagatctgct
     1681 gtgtccatgg caagaccgta acgtgcggaa aagtcggcga gacctgcggc atcaagctca
     1741 cggaacccct caagcacagc aacctttgca ggagcagcac aactcatcac aagagttgtc
     1801 ggcatcgcaa gatcggcctc acggctttcg atcgggttaa taacatattt tttgacccta
     1861 tcaagcacct catccgagat gtttccgcca agaacataga cgcgggccgt acggacaatg
     1921 ggacgttctt tctgggaaat aagctgaata cactgtgcag cactgtcagc acgctgatca
     1981 aactggcccg gcagatactc aacggcaaac atccgctcat cctctgcgag agcgggagtg
     2041 tctgtgacaa tatcaatctg cggctcagaa aacacagtgc tctttgcata cgcaaaaagg
     2101 tcggcatcga tgttatcgac atcgtagcgg ttgatgattc ggagagagga aactccctgc
     2161 actcccggca gagtggaaag ctcacggagg agcgcatgcg attcatttgc aagagcaggt
     2221 ttcttctcga caaatactct gaaaaccatt gtaaaatccc tacctgtcag cccggttagc
     2281 tgtatagtat ataggaggaa tgggtttttt agtttgctgt actgtgagag gaacaaatta
     2341 caaaaaagta caaagtaaaa agatcacttc tcccggtccg gggagaggct gctgcacatg
     2401 ttctcatgcg ggcaacagga gacacgaatg gctttaccgt tcactagtgc atcagcaatc
     2461 tttctccgcg cttcatgaag atcacgccat gcggttttgc gggacacacc aagcataccc
     2521 gctgcctcct cctgggagag attatccaga tcaacaagct tcaggaccgc aatctcttca
     2581 ggaaacacaa caaccgatgt cacggtttcc tgacggtggg gacagattgg ggcataacaa
     2641 ccggaggcac catcaagatc gatgcaccgt ttcacccgcg gtcggccgca tcttcctttt
     2701 ggacattcac tgggattttc cggcatgcta tctttcctgt atcatttttt tctctgtcgc
     2761 gtaaaagtag ttttgtccgt caacaaagac tgcttaaatt actcatatgc gccaaagtat
     2821 ataatagacc ctgataaaat actatgagag tattttccgg cagacgaaac tgcctgccgg
     2881 tattcgaggt agtgtgcaaa tgacgacgcc atttatcacc attgaaaatc tctgcatcga
     2941 ttttcccgca gatgcagaca atccggcagg cgagcaggtc aggattctta atgatatcaa
     3001 tctggaaata gctgaaggag agattgtcgg tgtgatcggc agatccggct gcgggaaaac
     3061 ggttctgatt cacctgttga gaggaattga acagccgcct accgcaggaa ggatcatcta
     3121 tcatatttcc cgctgtcctg catgcggcag aattgagttc cgcagcagtg caggcaagaa
     3181 gtgtccggcc tgcggtgcag tgcttgaagc acaggatgtg gacttctggg ctccggagaa
     3241 cgcagccatt aagacaaaga tcatggcaag gacttcgctg atgtttcaac ggacatttgc
     3301 cctctacggt gatgaccggg taattgaaaa tgttctgcgg gcactggatg atattgatta
     3361 ccccgcagag aaggcgatca accgtgcggc tgatctgatt gacgaggtca ggctgacaca
     3421 ccgtatgatg catgttgcgc gtgatctttc cggtggggaa aaacagcgtg ttgttcttgc
     3481 ccgtcaactg gcacgcgaac cgctattcct ctgtgcagat gaaccaaccg gaacacttga
     3541 tcccaagact gcgacgattg tgcatggtct tctaaagcat gcagcaaaga gcacaaacat
     3601 gggtatggtg atcacctctc acttttcgca ggtgctggag gatgtctgcg atcgtgcagt
     3661 gcttctggat gacggagtaa tcacaaagat cggtgagcct gccgagattg ttgcagagtt
     3721 catgaaggac tgtgaggatg atctggagta cagcgatcag gagatcggaa atccgatcgt
     3781 gcgggcagac aagctgtaca agaagttcat cgcagtggac cgcggcgtca tcaaagcagt
     3841 ggacaacatc acatttgaga tcaatgagcg ggagatcttt ggtgtcattg gtgtatcagg
     3901 cggcggaaaa acaacgcttt caaagatgat tgcaggcctg tatgaaccaa cggcgggaaa
     3961 actggatgtg cggattggtg acgaatggat cgatatgaca aaaccagggt accagttccg
     4021 cggtcgggct aaacagtaca taggtctgct gcatcaggag tatgatctct atccgcacag
     4081 gacgatcatc gataatctga ccgatgcaat cggcctcgag tttccaaagg agctggcagt
     4141 ccgtaaggcg atcattacac tgcggatggc aggttttacc gataaaaagg caaaagacgt
     4201 gctgaaccgc tacccgtcaa gcttgtcggc aggagagaaa caccgtgtgg cactggcaca
     4261 ggttctcatc cgtgaaccga gaattattct tcttgacgaa ccgaccggca ccatggatcc
     4321 gatcacgaag gtggatgtga agcactcgat cattcacgca cgcgaggaga tggatgagac
     4381 gttcatcatc gtgtcgcatg atatggagtt cgtccgtgat gtctgcgatc gctgtatgtt
     4441 catccgcggc ggaaagatca tcgatattgg tccgacgcat gaggtacttg caaaactttc
     4501 cgagcaggag atctcaacga tggccaaggc agttgcagag gaacgtgccg gtgctgagca
     4561 gtaacaatcc ctgaaaaata tttttttgaa aaaattatcc aaagcagacc cagcgatcgt
     4621 agatcgggtc acgatcgttt tcccagatct caaggtcgcg gtcaacacct tttgcccact
     4681 cacggaccca gtgtgcctcc tcctcggttg caaccgtgat gagtgtctcg gcggtgatcc
     4741 cagcaagctc ctcaacaatc ggctgccaga ggttctgcgt gtagacatac atctggccga
     4801 acatgatacc aaggccgcgt tccgcatccg gcagatatgc agatgcgaca gttccatcaa
     4861 tgcagatggt gtcctcagga gcaagacgtc cggatgcaag acccagtgcg agcagggcag
     4921 gatcgttgtc ataggcaatc gggcgaccac ccatctcgcg gataatcatg gaaccgatac
     4981 ctgatcctgc acatgcatca agacagagac ccggagcgga tgctccccag acttcatgca
     5041 ggagatcccg aaccttttcg attctgtcag gcgggagatc ttcccgtgca ggggcaattt
     5101 cctcacgaag tatctgtgaa aaatgcgcac gcacggcgtc atcaattgcg gcacgcggct
     5161 cctgatacag gtccccgtcc gcagcagtaa agagcgcgaa ctcctgttcc atcggactgc
     5221 ggaagttcca tgaagttccg acccactcat cgcccgcacg gaccgcaaac agcactggat
     5281 tctcctcctc atcaatcagc atccgggcac cccctggcgt tggttcggcc tcacagatta
     5341 gaataccagg ctcgccgttg ttcacaagtg aagcataatc aggttctagg aagcggagag
     5401 agtccagctc cagcatctct gcaacagatt tcattctttt ttaccctcaa accgtccgat
     5461 ggtcagacca cccggggaac ggatgatacc acttaccagt gcatcctcat gcagccatac
     5521 tttgtctccg gagacgtcac cgaggatatc ggcaccacgc tcgatcctga tattctgacc
     5581 aataacatca ccgtggactg cggtacgttc accgatcttg acacggcggg ttgcgtgaat
     5641 accaccaaag agggtcacat catttttcag ggtaatagcc cgagctttga tgtttccgtg
     5701 aagacgacag tttgcaccaa cttccattgg ggtggaaacg gagaagtaac ggagatccag
     5761 cgtggtgcgc ggaggaagaa cgagcggttc agagtttgct tctgatatga tggcattaac
     5821 agtcttctcc acctcttctt cattgtcaag gtgaagcatc gtcatgacat agaggagaag
     5881 atagaggatg acaggcatcg ggtttctgat actgatgtca ccgaacgctt tgaactcttt
     5941 atcgatggtg acgttgtcac cgatgtcaag gtttccgccg acggtcagct tcccgtgcac
     6001 tttgacacct tctccgagat aggcgtcacc gttgcagatg acagttccat tgatttcgca
     6061 gaagttgtca atgcgaagat cgccttcagc aacaacatct ccggtcagat tgcagaactc
     6121 acagacgacc acatcgtttc cgtacagccc aaacccgatg tcgcagtgat caccgaccag
     6181 aatatcattt tttgtcttga gaatgcgttc ctgcagttcg gttttgtccg gcaggataca
     6241 tttcttaaag agttcggcag ttgccatagt ggcaacggca gaggcatcct cgctgcgctg
     6301 tggttcttcg gcggggagtt cctctgtttc tgctgcggga gacggaggaa catcacgtat
     6361 ctgttccacc tcttctacag ggatctcctc cgcttcaatc aagacattct caggtatcgg
     6421 ctctgcaacg atgacccgtt tttttccctc tgccatactc caccctgtgt atatatctaa
     6481 taatcgttat gcgccgtact tatgggtttt gtcgatgagg tctaaaagga tcggcatcag
     6541 atcaccacca cgaatacgac aaagtgcacc agcggcacag gagatctcat cataagagga
     6601 tacctgatcg atccgtgctc cgtttccgtg aatgatcagc ggaacgggat cagcactgtg
     6661 ctctttgatg gtacacgggg ttgagtgatc accgcagaca acaatcagca actccggcat
     6721 ctccatgagg gggcggagct ccttatcgat cttttccaga aactcctttt tctccattgc
     6781 ttttccgtca tgtccatact cgtcggcatt tttgatgttg aagagtacaa agtcctgtgt
     6841 ttcaaattca cgcttgacca gcgaaacctg ttccgcaagc ggagtatccg gttttacggg
     6901 aatacggcga agaccaacag aactgccgat tcctgcaata agggcggctg cagctacaac
     6961 actcccagac agttcatgac gttcctcaaa cggttcgtac acccccattg ctccggcacc
     7021 acggatcagg acaacgtttg ccgggttttg cccggccgct ttgcgtgcaa ggttgaccgg
     7081 atggttcttg aggatctcct cggcctgacg gcaaaattcg ttacagactt cggcggtgaa
     7141 ttttgcttcg ggtgtatcct caccttcggg atgaatcatc tttggaggaa caccagttgt
     7201 tttaggatcg ttactggaaa ctgcagcaga cagtccagcc cctttcaagg cgagagcagc
     7261 acgatgaccg gttcccggct cgaactcaat ggtgacaccg tacttgctga ggtcaacacc
     7321 gtcacggatt gcggcagaga tctcagtagt atcggagatt cttcctgcac gccgatcgat
     7381 aacatttccg gcatcgtcaa gggttgcata gtttgcacgg aatccgatca tcccaggttc
     7441 catcctgata ccacatccgg cagcctcaag aggaccgcgg ccggtgtagt acttctgtgg
     7501 cggatagcca agcaggctta agtgtgaggt atctgatcct gcacggacac cgggtgcaac
     7561 cggatccatg attccgcaga tacctgttct tgcaagttca tcaaggttcg agatgtttgc
     7621 tgcctgaagc ggggttttgc cgccgagaca atcacatgga cggtcaccca caccgtcaat
     7681 gatgagaagg agaattttag atgctgtcat tacctgatag attggacggg taagactaag
     7741 tgtattgtgc ttttggaaaa atctacagta aaaacagtag cgaggaatgg atttgaacca
     7801 ttggtctacg ggttatgagc ccgtcgggat ctcctgacta ccccacctcg cttctttaag
     7861 aggtataata tgtatgacaa gtgcacataa ataaattccg atatgcatct tgcataccct
     7921 cattaggttg ttattcccat attcaggtat ggaagatgac gagttggaac agctgcgaaa
     7981 gaaacggttt gaacaactgg cagcaaagat accaccgccg gaagggtggc cggtcataga
     8041 actgactgag tacaacttca acgaggtaac acttggtcgg ccgaaagtgg tgattgattt
     8101 ctgggccgag tggtgcgggc catgccgata tttcagccca atatttgagg agatgtcggt
     8161 cgagtatccc gaagtacagt tctgtaaatg cgataccgat cgtaatcacg ggattgcaaa
     8221 acaacttggc attaccagta ttccgcgggt actgtatgtt ataaacggaa ctgcggtgcg
     8281 gctgcatgtg ggtgcaatgt ctgcggagaa gtttcgcgag gagttgaacg cagtgttccg
     8341 atccaatgac actgtttgag tcctttcacc caagtctgca ggaagttctg ctttcgggtc
     8401 tgggctggag tgggctgcgg ccggttcagg aggagacata ccggacggtt gccggaggcg
     8461 cggatgtggt ggtacttgcg ccgacagctg ggggaaaaac agagtcggca ttcattccgg
     8521 tgattgatgc tctgctgaag acaggttcca gaggacttgg agcgatctat ctatcgccgt
     8581 tgaaagccct gattaatgat caacaggaca ggattctgtt gatgtgcggg agggcaggac
     8641 tgactgtggc atcccagcac ggtgatgtag ccgcacgcga ccgatggaaa ttttctccag
     8701 aggcagacac cccgaatctt ctgctcacaa cacccgaatc actggaagta ctgcttggag
     8761 atcccaattc acggagtgca ttctcctcac tccggtttgt cgtgatcgat gagattcatg
     8821 catttatgga aacggatcgt ggcgtacatc tccggtgcct tctggatcgc ctggaaagga
     8881 tcacaaaaaa tactgttgtg cagaggatcg ggctctcggc aacggttgga aatccggagg
     8941 aactgcttgc ttggatgtcc gggccaaatc gaaagaaaca actagttcag attccgtccc
     9001 ctgcaacacc gaaacagttc tcttttgtag tggaggagga gtttactgca cagatacggg
     9061 cggtagcaga tgcagtacgg ggaaaaaagg cactggtgtt tgtagacagc cggagttttg
     9121 cagaacggct gatggcaccg ctcagtgatc tcgtccccgg agtgtatctt catcactcgt
     9181 cggtgtcagc agagga
//
LOCUS       DALTZZ010000099         2230 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_14107,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000099 DALTZZ010000000
VERSION     DALTZZ010000099.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14107"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="O0X12_01810"
     CDS             complement(<1..225)
                     /locus_tag="O0X12_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52928.1"
                     /translation="MNNEEPTRSDEWSGCTRESTTDWALYHMLTEDKPVMISELVERS
                     GWNRDVVAASLSRLEDACLIAVVGEAVSLLS"
     gene            315..1082
                     /locus_tag="O0X12_01815"
     CDS             315..1082
                     /locus_tag="O0X12_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52929.1"
                     /translation="MTDIDMELTVVVAFTSLVVSIISSIYGFTQNRKLQEFEHRTRED
                     TKEDLVKLDTALKSIIIKSSYRNDMRNLVFDAEKNIISDFLLSPTWLAIEYLINQNGD
                     KTVLHVELLNILNRQDGITIGFAAHNAERELSRISSQYQEELLNLKDNIYTMLPEISG
                     DSVDRLSKTAVSAVKDRITYNDDYVKDAFRYLKEVKGVTDPNIDLFLAVLDNNEELLK
                     QSIDAGGNLAMDDKELIKKYEKLLKDHKFVRKQELPA"
     gene            1159..1572
                     /locus_tag="O0X12_01820"
     CDS             1159..1572
                     /locus_tag="O0X12_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52930.1"
                     /translation="MAQPCSYAELTLLADDLYNRCNDDDAELARLMHELDPDTAVQLC
                     TSDLSNAAQAYMYAFNEIPDDDVYDILLLEPSSQLLHGIKIQPVELAELIMGFDKYKK
                     IFFIGVWDGEKIVAAFTGDGAYDRAAAFAREHCSA"
     gene            complement(1575..>2230)
                     /locus_tag="O0X12_01825"
     CDS             complement(1575..>2230)
                     /locus_tag="O0X12_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HJK52931.1"
                     /translation="PAHIAAAKAGMLSGRADLESYLRTDPFFGTSYSPVPAEPDAPLI
                     VHRMADAAEEACVGPMAAVAAAIAWAGVESMVASGASFGLIDNGGDIVFTAPDRSVRV
                     GLYAGDAPVSGKYAFVLQPAPGIRGVCTSSATVGPSVSFGVADAVVVFSHNPAKADAW
                     ATGLCNELRPTDVGICIPEGTAVDGVYAVIGEWTSVWGTVPEIVPATVRYDMITKGE"
BASE COUNT          638 a          596 c          506 g          490 t
ORIGIN      
        1 acttagaagg ctcaccgcct cgcccacaac cgcaataagg caggcatcct caagccggga
       61 cagagatgcc gctacaacat cacgattcca cccggatcgc tccaccagtt cggaaatcat
      121 caccggctta tcctctgtaa gcatatggta gagtgcccag tcagtagtac tttcgcgtgt
      181 acagccactc cactcatcgg agcgggtcgg ctcctcattg ttcatgagat aacagtttgc
      241 acgcggaaga gattaaggat gtatgcttag gatcacgcat cttgtcacaa acaattcctt
      301 tatcactgga aaagatgaca gacatagata tggaacttac cgtcgttgtc gcatttactt
      361 cccttgtggt cagcatcatc tccagtatct acggattcac ccagaataga aaactccagg
      421 agtttgaaca ccggacacgc gaggacacaa aagaggatct agtaaaactt gataccgcac
      481 tcaaatccat catcatcaaa tcctcctacc gaaacgacat gagaaatcta gtatttgatg
      541 cggagaagaa tatcatcagt gatttcctct taagtcccac ctggcttgca atagagtatc
      601 taattaacca aaacggtgac aaaaccgtac tgcacgtaga actcctgaac atcctcaaca
      661 ggcaggacgg tatcaccata ggctttgccg cccacaacgc agaacgggag ctcagcagaa
      721 tcagcagtca gtaccaggaa gaactgctaa atcttaagga taacatctac accatgcttc
      781 cggagatttc cggagacagt gtggatcgtc tgtcaaagac agctgtgagt gcagtaaaag
      841 acagaatcac ctacaacgat gactacgtca aagatgcgtt ccgctatctc aaagaggtaa
      901 aaggggtgac tgatcccaac attgatctgt tccttgcggt tctggacaac aacgaggagc
      961 tgctcaaaca gtccatcgat gctggaggaa accttgcaat ggacgacaag gagctgatca
     1021 agaagtacga gaagcttcta aaagatcata aatttgtcag gaaacaggag ctgcctgcat
     1081 agattattca tctgagagat acgtatacta gacagaaatg actgcgatga gtggtgaccg
     1141 caaaaggagg ggaaatgtat ggctcaaccg tgcagctatg cagaactgac gcttttggca
     1201 gatgatctct acaaccgctg taatgatgac gatgcggagc ttgcacggct gatgcacgaa
     1261 cttgacccgg acaccgcagt acaactctgt acatcagacc tgtcaaacgc ggcacaggcc
     1321 tatatgtatg ccttcaatga aattcctgat gatgatgtct atgacatttt gttgcttgaa
     1381 ccatcatccc agcttctgca tggcatcaaa atacagccag ttgaacttgc ggaactgatc
     1441 atgggatttg acaaatacaa aaaaatattt ttcattggtg tgtgggacgg ggaaaagatt
     1501 gtggcggcct tcaccggaga tggtgcatac gatcgtgccg cagcttttgc acgtgagcac
     1561 tgctcggcat aattctactc gccttttgta atcatatcat accgaacagt agccggaaca
     1621 atctccggta cagtacccca taccgaagtc cactcaccaa taactgcata cactccgtca
     1681 acagccgttc cttccggtat gcagattccc acatctgttg gtctaagttc attacataac
     1741 cccgttgccc aggcatccgc ttttgccggg ttatgcgaaa acaccaccac tgcgtccgca
     1801 accccaaacg aaaccgacgg tccgaccgtt gcggacgatg tacatacacc acgtatacca
     1861 ggggcaggct gcagtacaaa cgcatactta ccggacaccg gagcatcccc tgcatacaga
     1921 cctacccgta cagatcggtc aggtgctgtg aacacaatgt caccaccgtt gtcaatcagg
     1981 ccaaacgaag ccccggaagc aaccatactt tccactcctg cccacgcaat cgccgcagca
     2041 acagcagcca ttggcccaac gcacgcctct tctgcggcgt ccgccatcct atgtacaatt
     2101 agcggggcat ccggctccgc cggtaccggt gagtaggatg ttccaaaaaa aggatcagtc
     2161 cggaggtagg actcaaggtc agctctgcct gaaagcatcc ctgcttttgc cgctgcgata
     2221 tgggcaggat
//
LOCUS       DALTZZ010000100         4983 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_15022,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000100 DALTZZ010000000
VERSION     DALTZZ010000100.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15022"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="O0X12_01830"
     CDS             complement(<1..430)
                     /locus_tag="O0X12_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HJK52932.1"
                     /translation="MAENGCANPVWPCAMTGAVATLAGFPDLCVIIHGSSGCYYYPRS
                     LLKVPLFSTYLLESEIVFGTIDRLHEVVTSVAATGKPIAVVNSCVPALTGEDLKTALS
                     GETAIFVDAPGFCGNAEEGAEKVFSTVNPVVDASAAGVNID"
     gene            complement(423..1469)
                     /locus_tag="O0X12_01835"
     CDS             complement(423..1469)
                     /locus_tag="O0X12_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HJK52933.1"
                     /translation="MPDNIYEARMAGCTLGGALSVACFIPGAVAVVHAPQGCVHQTFS
                     MLHAMANESEIWTVPEILVSDIGDREVIFGGEERLSEVLDVAAEKNPDLILVVTSCVP
                     ETIGDDCGAVCARHPYADRIVYVPTSGFLGGSAKDGENAVLAALAVRAPVCDPVPGTV
                     ALVGEKNLESEADQNYAEVERLLARLGLRVCVRFCRNCTYATLMQLGTAACFVLRDER
                     AADAGKVIAERFGRPVIPEFPRGLSGCLAFLRAVGTACGVSEEKIEAAVSAESAYQDA
                     MLAKFSPLSGTTVFLGAEPFAGTLAVAREAAARLGMREDANGTEVRLPFYLPVGTAGV
                     EKMLYLWRRSVRYG"
     gene            complement(1450..2256)
                     /gene="cfbC"
                     /locus_tag="O0X12_01840"
     CDS             complement(1450..2256)
                     /gene="cfbC"
                     /locus_tag="O0X12_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase ATP-dependent reductase subunit"
                     /protein_id="HJK52934.1"
                     /translation="MRQIALYGKGGIGKSTTSANLSAAFSEMNLDVMQIGCDPKHDST
                     RMLMHGRWIPTVLEQMYERKDLSADDVIFTGFGGVRCVEAGGPEPGIGCAGRGIIATF
                     QLLEKMKALYGDVVVYDVLGDVVCGGFAMPMRDGYAQEVYLVTSGELMSLYAANNICK
                     AVARLSQRSTARCRLGGVICNSKNMDNERELVAEFASRIGSNLVQFIPRDKAVQAAEV
                     NQMTVIEHAPQSPQAAVYRSCAKTILENEDLRIPKALELDELEDLARQYL"
     gene            complement(2329..3816)
                     /locus_tag="O0X12_01845"
     CDS             complement(2329..3816)
                     /locus_tag="O0X12_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HJK52935.1"
                     /translation="MGIVRELASVGHDLGAVFLLIGLATILPLGVGAYYQEWDAFSVM
                     GQTTLLYLILGGALRFLPKGDTKSRNSLSIAATALVWVFVSFLGCLPFLFTGMNLLDA
                     AFESMSAWTGTGFSLATNIEEWPKTLLLWRSFMQWIGGLGIIALTLTVVNKAGLVTRG
                     LYRSEGRSEAFMPSVIATAFQMWKIYVVLTVISLLAIMATGLNLWDAVNVAFCAISTG
                     GMSIYANGIMHFQNLGLEMVLIPIMLAGALPFRLYYLAYTNHSFKEILSDRVLHLLVT
                     VFLLVAAFLTCDLVLFNDYTLLDAVREGFFMAGTAISSTGFQNTTLAGWEMASILFLA
                     VFLLIGGAPGSTAGGIKLDRVQVMFEAMIWWFRRTVVSSRAYILMRHDGKTVAGKDAD
                     MLIAKSLLLIILYILLVVATLVILLHDPYFSKDVMGTVYDVFSCVGNNGSSAGIVGAG
                     MPDYAKVVMYVVMWISRLEIIPVIILVWGLLRKFEWHLGSGPSKR"
     gene            complement(3910..4623)
                     /locus_tag="O0X12_01850"
     CDS             complement(3910..4623)
                     /locus_tag="O0X12_01850"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HJK52936.1"
                     /translation="MKQGDLLYLGKAKSVYLTENPDELLVVFRDDITAFDGQKKNVLS
                     GKGGYNAKVTAYFYPLLEAAGIPTHFLAAESANMHRVRKLAMIPLEVIVRNRAAGSLV
                     KNYGFTEGQPLSPPLIVMDLKDDSRHDPMVNDEIILALGLATAEELAEIKRLALAINA
                     VLKENLAAKDLDLIDMKFEFGRDAAGTIRLGDEISMDSLRMWDHGTIGASLDKDVYRK
                     ELGDVMETYAAVARRICGE"
     gene            complement(4670..>4983)
                     /gene="cofG"
                     /locus_tag="O0X12_01855"
     CDS             complement(4670..>4983)
                     /gene="cofG"
                     /locus_tag="O0X12_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833956.1"
                     /note="7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase
                     subunit 1; catalyzes radical-mediated transfer of
                     hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to
                     5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form
                     7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO); functions
                     in F420 biosynthesis along with cofH; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofG"
                     /protein_id="HJK52937.1"
                     /translation="ADTIRLAAEILPNDISIQIPPNLADAGKLLSLGVTDLGGVSPVT
                     IDYINPEHPWPALDALADIAAGYELRERLCVYPQYCNAEWISPELLPLVKRLAERVYS
                     E"
BASE COUNT         1230 a         1553 c         1167 g         1033 t
ORIGIN      
        1 cgtcaatgtt tactcccgca gcactcgcat ccaccaccgg gttcacggtg gaaaacacct
       61 tctccgctcc ctcctccgca tttccgcaga acccaggagc gtccacaaag atcgccgttt
      121 caccggaaag cgccgtcttc agatcctcgc ctgtcagcgc aggcacacag gagtttacca
      181 ctgcaatcgg ctttcccgtt gctgccacac ttgtcaccac ctcatgcagc cgatcgatcg
      241 tgccaaacac aatctccgac tccagaagat acgtactaaa tagcgggact ttcagaagcg
      301 accgcgggta atagtaacag ccggatgacc cgtgaatgat cacacagaga tccggaaaac
      361 ccgcaagcgt tgcaaccgcc ccggtcatcg cacacggcca caccggattt gcacaaccgt
      421 tctcagccat acctcacgct cctccgccac aaatacagca tcttctccac tcctgccgtt
      481 cctaccggca gataaaacgg cagccggacc tcagttccgt ttgcatcctc cctcatccca
      541 agccgtgcag cagcctcacg ggcaaccgca agcgttccgg caaacggctc ggccccaaga
      601 aacaccgtcg ttccggaaag cggtgaaaac tttgccagca tcgcatcctg atatgccgac
      661 tccgctgaaa ccgctgcctc tatcttctcc tccgaaacac cgcaggccgt gccgaccgcc
      721 cgcagaaacg caagacagcc ggaaagaccg cgggggaact ctggaataac cggacgcccg
      781 aaccgctcgg caatcacctt tccggcatcc gccgcccgtt catcccggag aacaaaacac
      841 gcagccgtac cgagctgcat cagcgttgca tacgtacagt tccgacagaa ccgcacgcaa
      901 acccgcaggc ccagccgggc aagcagccgc tccacctccg catagttctg atccgcctca
      961 gactccagat tcttctcccc aaccagcgcc accgtccccg gaaccggatc acaaaccggt
     1021 gcacggacgg caagtgccgc aagcaccgca ttctccccgt cctttgcact cccgcccaga
     1081 aacccggacg tcggcacata cacaattctg tccgcatacg gatgacgggc acacaccgcc
     1141 ccgcagtcat ccccgatcgt ctccggcaca caggacgtca ccaccaaaat caggtcagga
     1201 tttttctccg ccgcaacatc cagcacctcc gacagccgct cctcaccgcc gaagatcacc
     1261 tcgcggtcgc cgatatcgga gacaagaatc tccggcaccg tccatatctc gctctcattc
     1321 gccatggcat gcagcatgga aaacgtctga tgcacacacc cctgcggtgc atgcaccaca
     1381 gctaccgctc cgggaataaa acaggcaacc gacagggcgc cgccaagcgt acaccctgcc
     1441 atccgtgctt cataaatatt gtctggcaag atcttccagc tcgtcaagtt cgagagcctt
     1501 tggaatgcgc aaatcctcgt tctccagaat cgtttttgca cagctccggt ataccgcagc
     1561 ctgcggtgac tgcggtgcgt gttcgatcac cgtcatctga ttcacctcgg cagcctgtac
     1621 tgccttgtcg cggggaataa actgcacaag attcgatccg atccggcttg caaactctgc
     1681 aacaagttca cgctcattat ccatattctt tgagttgcag ataacgccac cgagcctgca
     1741 ccgtgcagtg ctccgctggg acagacgggc aaccgcttta cagatgttgt ttgcggcata
     1801 caaactcatc agctcaccgc ttgtcaccag atacacctcc tgtgcatacc cgtcccgcat
     1861 cggcatcgca aacccgccgc agaccacatc gcccagcaca tcgtacacaa cgacatcacc
     1921 gtacagtgcc ttcatcttct ccagcagctg aaacgttgca ataattcccc ggcctgcaca
     1981 cccgatcccc ggctccggtc cgcctgcctc cacacaccta acaccgccaa atccggtgaa
     2041 gataacatca tcggcactga gatcctttcg ctcatacatc tgctccagaa ccgtcggaat
     2101 ccagcgtccg tgcatcagca tccgggtact gtcatgcttg ggatcacagc caatttgcat
     2161 gacatccaga ttcatctccg aaaatgccgc tgaaagattt gcggatgtcg tggatttccc
     2221 gatacctccc ttgccgtaga gggctatctg tctcattcac tacctatttg actgcatgcc
     2281 acatatattt tgagttgtgg caatattcct ttctctacgg gggactcttc accgctttga
     2341 cggtccgctt cccagatgcc actcgaactt acggagcagg ccccagacaa gaataataac
     2401 cgggatgatc tccagtctgc tgatccacat gaccacatac atcaccactt ttgcatagtc
     2461 cggcataccg gccccgacaa ttcctgcgga acttccgttg tttccgacac aactgaaaac
     2521 atcgtacacc gttcccatga catctttgga gaagtaggga tcatgcagca ggataaccag
     2581 cgttgcaaca accagcagaa tatacaaaat gatcagcagc agggactttg caatcagcat
     2641 gtcagcatcc tttcctgcaa cggtctttcc gtcatggcgc atcaggatgt atgcccgtga
     2701 actgacaact gttctcctaa accaccagat cattgcctca aacatcacct ggacacggtc
     2761 cagtttgata cctccggcag tacttccggg cgcaccgccg atgagcaaga acactgccag
     2821 gaacagaatc gatgccattt cccatcccgc aagggtggtg ttctggaatc cggtcgaact
     2881 gattgcagtt cccgccataa agaatccttc ccggacagca tcaagcagcg tgtagtcgtt
     2941 gaacagcacc agatcacacg taaggaatgc agcaaccaga aggaataccg taacaagcag
     3001 gtgaagaacg cggtcggaaa gaatctcttt gaaggagtga tttgtgtagg caagataata
     3061 gagacggaac ggcagggcac ccgcaagcat gatcgggatg agcaccatct ccagtccgag
     3121 attctggaag tgcataattc cattcgcata aatggacata cctcccgtgg agattgcaca
     3181 gaacgcaaca ttcaccgcat cccacagatt caggccggtt gccatgatgg caagaagcga
     3241 gatgaccgta agaacgacat agatcttcca catctgaaag gcggttgcga tcacacttgg
     3301 cataaacgct tccgaccgtc cttccgaccg gtagaggccg cgggtgacaa gacctgcttt
     3361 gttgaccaca gtaagggtaa gtgcaatgat tcccagtcct ccgatccact gcataaagga
     3421 acgccacaaa agcagtgttt tgggccactc ctcaatgttt gtagcaagcg agaatcccgt
     3481 tcccgtccat gccgacatcg actcaaacgc cgcatccagc agattcattc cggtgaacaa
     3541 aaacggcaga cacccaagaa aactgacaaa aacccagaca agtgctgttg ctgcaatgga
     3601 caaactgttt cgcgacttgg tatccccttt gggaagaaaa cgcagggcac cgccgagaat
     3661 gaggtacagg agtgttgtct gtcccatcac agagaatgcg tcccactcct gataatatgc
     3721 cccgaccccg agcggcagaa tggttgcaag gccgatcagg agaaaaaccg ctcccagatc
     3781 atgtccgaca cttgcaagct cacgaacgat gcccatttga tatatgatga tcgaacctgc
     3841 tcaaatataa tgatacggga tcgattgtct gcatcacccg aatttgttta caaaaaagaa
     3901 aaaatgaaat tattcgccgc agatgcgccg ggcaaccgct gcataggtct ccatgacgtc
     3961 gccaagttcc ttgcggtaca cgtccttgtc cagcgatgca ccaatggtac cgtgatccca
     4021 catccgcagg gagtccatgc tgatctcatc acccagccgg atcgttccgg cagcatccct
     4081 gccgaactcg aacttcatgt cgatcaggtc caggtctttt gctgcaagat tctcctttag
     4141 tactgcattg atggcgagtg ccagccgttt gatctctgca agttcttctg cggtggcaag
     4201 gcccagtgcc aggatgatct catcgttgac cattggatca tgccgggagt cgtctttgag
     4261 atccattaca atcagggggg gagataacgg ctgcccctcg gtaaatccgt agtttttcac
     4321 cagcgatccc gcggcacggt tgcggacaat tacctccagc gggatcattg caagttttcg
     4381 gacccggtgc atgttcgctg attcggctgc aagaaaatgc gttgggattc cggcagcctc
     4441 cagaagggga tagaagtatg ccgtaacctt tgcattgtac ccgcccttgc cggaaaggac
     4501 gttcttcttc tgtccgtcaa acgcggtaat atcatcccgg aacactacca gaagctcgtc
     4561 tgggttctcg gtcagataca cggactttgc cttacccaaa tagagaagat cgccctgttt
     4621 catgatgcca gatctattgc acggcaacca tgataagtgt tagcatgaat cactcggaat
     4681 aaacccgttc tgcgaggcgt tttacgagcg ggagaagttc cggcgaaatc cactcagcgt
     4741 tgcagtattg gggatacaca cacagtcgtt cccgcagttc gtatcctgcg gcgatgtctg
     4801 caagggcgtc cagtgccggc caggggtgtt ccgggttgat gtagtcgatg gtaaccggcg
     4861 aaacaccgcc aagatcggtg actccgaggg agagcagttt tcctgcgtcc gcaaggttcg
     4921 gcggtatctg aatcgagata tcgtttggca gaatctcggc tgcaagcctg atggtgtcgg
     4981 caa
//
LOCUS       DALTZZ010000101         1580 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_15529,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000101 DALTZZ010000000
VERSION     DALTZZ010000101.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15529"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="O0X12_01860"
     CDS             complement(<1..863)
                     /locus_tag="O0X12_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LarC family nickel insertion protein"
                     /protein_id="HJK52938.1"
                     /translation="MRLLVIDPGFGAAGDMMCGALLAAGADRNTVLCAMRSVAPDLSV
                     SQVVRCGMPALYVQTHTGPAHRTLDEVLAIVRAADAPAAAVSLAERVFSRTAAAEEVV
                     HGTHHVHFHEVGADDAIADVLGACTALVTLGVDAVHILPLSVGSGTIVCAHGTMPVPA
                     PATAEILRNSDLQVMIGGFAGELCTPTGAALLAEFSASFGTKDHVGKIVSTGCGAGTR
                     DPAEYPNVLRAMVLVTEARFFAPAVDVLETNVDDVSGELLSDTVSSLMSAGARDACLV
                     PVVMKKGRPGY"
     gene            983..>1580
                     /locus_tag="O0X12_01865"
     CDS             983..>1580
                     /locus_tag="O0X12_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HJK52939.1"
                     /translation="MPEISLKVDSAYPEDQGSGKARLDPETMQKLGILPGQLVVIEGK
                     RKTVAKVWRAMTADWKQEKIRIDKFTRINAGVNPGDRVLVRPVEEQIEADRVCLIPPI
                     RMPQNYDAEPEEIVESLINFPITEGDILPIITNLPNTYLEFKIASIEPEGACIISRNT
                     DIEISDEGDLEGFEGTKQISYEDIGGLKRELRRVREMIE"
BASE COUNT          459 a          436 c          369 g          316 t
ORIGIN      
        1 atatagccag gccgtccctt tttcatcacc accggaacca gacatgcatc tctggcacct
       61 gcactcatca gagatgacac agtatctgag agaagttctc ctgacacatc atccacgttt
      121 gtttccagca catcaacggc gggggcgaag aaccgggctt ctgtaacaag taccattgcc
      181 cgcaggacat tcgggtactc tgccggatca cgggtacctg ccccgcaccc agttgaaaca
      241 atctttccaa catgatcctt cgttccaaaa gacgcggaaa actctgcaag aagtgctgca
      301 cctgtcggcg tgcacagttc accggcaaaa ccgccgatca ttacctggag atccgagttt
      361 cgtaaaatct cggcggttgc aggagcagga accggcatag tcccgtgggc acaaactatt
      421 gtaccggatc caaccgacag cggcagaata tgtactgcat ccaccccaag tgtcacaagt
      481 gccgtacatg ccccaagcac atctgcaatc gcatcgtcag caccaacttc atggaaatgc
      541 acgtggtgtg taccatgaac aacctcttca gcagcagcag tcctggaaaa tacccgttcg
      601 gcaagagaaa cggcagcggc aggagcatct gctgcacgga caatcgccag tacctcatca
      661 agtgtcctgt gcgcaggtcc agtgtgggtc tggacatata gtgcaggcat accacaccgc
      721 acaacttgtg agaccgatag atctggagct actgaacgca tggcgcaaag aacagtattc
      781 cgatcagcac ctgcagcaag aagtgcgccg cacatcatgt caccggcagc accaaaccct
      841 ggatcaatca ccaacagacg catagaagaa tcttgaataa tactcttgca ttcccggaat
      901 aaaattctcc ccattagaca gatgaaatac tgctccctcc agaatattat taatcaattc
      961 cggaacactg tactactcaa ttatgccgga aatatcactc aaagtcgaca gtgcctatcc
     1021 tgaggatcag ggatcaggaa aagctcggct cgacccagag actatgcaga aacttgggat
     1081 actccccggc cagcttgtcg tcatcgaagg aaagagaaaa acggttgcca aagtctggcg
     1141 tgccatgact gcggactgga aacaggaaaa gatccgcatc gataagttca cgcggataaa
     1201 cgcaggggtc aaccctggag accgtgtact tgtccgaccg gttgaagaac agatcgaagc
     1261 agaccgtgtg tgtctcattc ctcctatccg catgccccaa aactatgatg cggagccgga
     1321 agaaatcgtt gagtctctta tcaacttccc gataacagaa ggagacattc taccgatcat
     1381 caccaacctc cccaacacct accttgagtt taaaatagca tcaattgaac cggaaggtgc
     1441 gtgcatcatc tcccgtaata cggacatcga gatatccgat gaaggagatc tagaagggtt
     1501 cgaagggaca aaacagatca gctatgagga cattggcgga cttaaaagag aactgcgccg
     1561 cgtccgtgag atgattgaac
//
LOCUS       DALTZZ010000102         2863 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_15622,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000102 DALTZZ010000000
VERSION     DALTZZ010000102.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15622"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="O0X12_01870"
     CDS             complement(<1..366)
                     /locus_tag="O0X12_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52940.1"
                     /translation="MEEDMITSCRGYRYQNGNVTEIDDVVVREDTITLFLNGREYLRM
                     AATCDMLTELGAGFFTAAGIAERILSGRVEGTKVFVEAELRCDVTPDGFAPAKPARCI
                     TTEGVQIAPNEIFTLREALN"
     gene            503..1720
                     /locus_tag="O0X12_01875"
     CDS             503..1720
                     /locus_tag="O0X12_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-related McrA-glycine thioamidation protein"
                     /protein_id="HJK52941.1"
                     /translation="MIELQPSPKTYCGQTERSKTPEETLAFIEPLTKIAGITRVADIT
                     DLDRIKIPVYSCIRPTAADGAISVYNGKGGTEAEARVAGIMEGIERYSAEAMPRDLTL
                     VSYDMLSLTEDALNPVDLILPKNIDPHAELPWVDAWDITNNRSIKVPLCAVMHPNPHY
                     MPSLFRSSSNGIASGNTREEAIFYALTELIERDAWSLVETTRITGPAVTGLTGAAAEM
                     LAKFTAAGVEVTLKDITSDIGIPTIAAVADDVTLKDPRLLTIGMGTHTNPEIAVIRAL
                     SEVAQSRATQIHGAREDATIAAFREMMGYDRVKRMNAYWFRVEETRDFSEIPGCATSD
                     FRTDILAIIDRLRAAGLNRVLVVDLTDPELGVPVVRVVVPGLECFTIDNERRGKRCLD
                     AERRRLHRSKSSA"
     gene            1677..2315
                     /locus_tag="O0X12_01880"
     CDS             1677..2315
                     /locus_tag="O0X12_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-related McrA-glycine thioamidation protein"
                     /protein_id="HJK52942.1"
                     /translation="MPNAAVFIGPSLPLEEAKQILLDAEYLPPAKRGDLTEAVRAGAK
                     IIVLIDGVFFQDRAVGHREILSALRAGVAVYGSSSMGALRASEMDTLGMVGVGSIYCW
                     YRDGIIIADDEVGLVYDPETHVALSEPMVNIRATFAKARASDIIDDAEEKELLRACKA
                     IYYQDRTYRRVLRDAEISADKKTTLAAWIKTNAVDQKRLDAIACLNTVRDAL"
     gene            2312..>2863
                     /locus_tag="O0X12_01885"
     CDS             2312..>2863
                     /locus_tag="O0X12_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HJK52943.1"
                     /translation="MTERYLRQIPVIGDDGQKKLEEATVFLAGCGGLGSPVAFYLAAA
                     GVGHLRIADCDVVEETNLNRQILHRPDRIGMPKAASAKLTLEAFNPDIDVTAFSAFID
                     RNTVRRMIGDADIIVDAMDNFSARYILNEISQTLDIPLMHGGVAGLTGQVTLIIPRKT
                     ACLSCIFPDAKTTTVTPILGAAAG"
BASE COUNT          674 a          741 c          714 g          734 t
ORIGIN      
        1 gttcagtgct tctcgaagtg taaaaatttc gttgggtgca atctgtaccc cttctgtcgt
       61 aatacaacgg gctggttttg ctggtgcaaa cccgtccggt gttacatcac accgcaactc
      121 cgcctccaca aacacctttg ttccctctac acggcccgat aaaatacgtt ccgcaattcc
      181 tgctgccgtg aaaaatcctg caccaagctc tgtgagcatg tcacaggttg ctgccatccg
      241 cagatactct cttccgttca gaaacagtgt aattgtatct tcgcgtacca caacatcatc
      301 aatctctgtg acatttccgt tctgatatcg gtatcctctg caggaagtaa tcatatcctc
      361 ttccatgttt cccctctttt cacggcgtga atgcccatat gtttatacga acaggatata
      421 aattcagcga acgaactcaa aaactgaact ggcgtacgac aacgaaaccc tcaatagcct
      481 tcgcgcccga acaatgatga taatgataga gcttcagccg tctcccaaaa cttactgcgg
      541 acagaccgag cgaagcaaaa ctcctgaaga gacgcttgca ttcatcgaac cgctgacaaa
      601 gatcgcgggc atcacccgtg ttgccgatat cactgacctt gatcggatta aaatcccggt
      661 atactcctgc atacgtccga ccgctgcaga cggtgcaatt tcggtataca atggcaaagg
      721 aggaactgaa gcagaagcac gtgttgccgg tattatggaa gggattgagc gttactcagc
      781 tgaggccatg ccacgcgatc tgacgctggt gtcgtatgat atgctctcgc tcactgaaga
      841 tgcccttaat ccggttgatc tcatactccc caagaatatt gacccccatg cagaacttcc
      901 ctgggtggat gcgtgggata ttacaaataa ccgttccatc aaagtcccgc tctgtgcggt
      961 tatgcatcca aaccctcact acatgccgtc gctgtttcgt tccagttcga acggtattgc
     1021 atccggcaat accagagaag aagcaatctt ctatgctctt actgagctta tcgaacgtga
     1081 tgcctggtcc cttgtggaaa ccacccgtat cactggtccg gctgttaccg gccttaccgg
     1141 agctgcggca gagatgcttg caaagtttac tgctgcaggg gtggaggtca ccctcaaaga
     1201 catcacttca gacattggta ttccgaccat tgccgcggtt gcagatgatg tcaccctcaa
     1261 agatccgcgt cttctgacta ttggaatggg cactcacacg aaccctgaaa ttgctgtcat
     1321 acgcgcgtta tccgaggttg cccaaagccg tgcaacccag attcacgggg cacgggaaga
     1381 tgcaaccatt gctgcatttc gcgaaatgat gggatatgat cgcgttaagc gcatgaacgc
     1441 atactggttc cgcgtggaag aaactcggga cttctctgag atccctggct gtgcaacgtc
     1501 agacttccgg acagatattc tggcaattat cgatcgtctg cgtgcagcgg gacttaatcg
     1561 cgtactggtt gttgacctca ctgatccaga gcttggtgtg ccggtagtac gtgtggtcgt
     1621 cccggggctt gagtgtttca caattgataa tgaacgcaga ggaaagaggt gccttgatgc
     1681 cgaacgccgc cgtcttcatc ggtccaagtc ttccgcttga ggaggcaaaa cagattttgc
     1741 tggatgccga gtatctgcct ccggcaaagc gcggtgatct caccgaggct gtcagggcag
     1801 gtgcaaagat tatcgtctta attgacggtg ttttcttcca ggatcgggcc gttggccacc
     1861 gcgagatcct cagtgctctg cgggcaggtg ttgccgtgta cggttcctct tcaatgggtg
     1921 ctttacgggc gtctgagatg gacactctag gtatggttgg cgttggttcc atttactgct
     1981 ggtatcgcga cgggattatc atcgctgatg acgaagtggg tctcgtctat gatcctgaaa
     2041 cccatgtggc actttctgag cccatggtca atatcagggc aacatttgca aaagcccgtg
     2101 catcagatat tattgatgat gccgaagaga aggaacttct tcgcgcctgt aaagcgattt
     2161 attatcaaga tcgcacttat cggcgtgttc tccgtgacgc agaaatttct gctgataaaa
     2221 aaacgactct tgccgcttgg atcaaaacga atgcggtgga tcagaaacgg cttgatgcca
     2281 ttgcctgcct gaatacagtg agggatgcct tatgacagag cgctatctcc gtcagatccc
     2341 tgtgattggg gatgacggac agaaaaaact tgaggaggca acagtgtttc ttgccggatg
     2401 tggtggactt ggatctcctg ttgcctttta tcttgcagct gcaggggttg gtcatctccg
     2461 cattgctgac tgtgatgttg tggaggagac caaccttaac cgccagatcc tgcaccggcc
     2521 cgacagaatt ggtatgccga aagctgcgtc cgcaaagcta acgctggaag ctttcaatcc
     2581 ggacattgat gtaacggcat tttctgcctt cattgataga aacactgtcc gccgtatgat
     2641 tggggatgct gacatcatcg ttgatgcaat ggacaatttt tctgctaggt acatcttaaa
     2701 cgagatctcc cagacgcttg atatcccgct catgcacggt ggtgttgccg gccttaccgg
     2761 tcaggtaact ctgatcattc ctcggaaaac tgcctgtctg tcctgcatct tcccggatgc
     2821 gaaaacaaca acagttacac cgattctggg tgccgctgcc gga
//
LOCUS       DALTZZ010000103         2182 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_15775,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000103 DALTZZ010000000
VERSION     DALTZZ010000103.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15775"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="O0X12_01890"
     CDS             <1..422
                     /locus_tag="O0X12_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52944.1"
                     /translation="IKYYHDTEAVDIPEDVTVLPVTVDSSVYLPELHGIARGEELIGV
                     PLWLISYKVKHNIYMIEVEAHSGLVLPEWHPIKEPVNWKKTAMTAFIPMFLLSLVAIY
                     FNPWIFVIVVILLFIFLYQSEMLGLITMKEREEPHGP"
     gene            412..1908
                     /locus_tag="O0X12_01895"
     CDS             412..1908
                     /locus_tag="O0X12_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52945.1"
                     /translation="MGLERSVEDRVGRVMYRSKVTKGVADRTIRDWFTKGIKAPDLPN
                     KAVMTELYMIYIPFWRFVAQGKAVACGYSEYNEKTGNTIRNIYKELIDDEFVWTEVAC
                     DTGKYGLTKLWLEPGGEVPYTPGAVVSMEPGGSAVEASRRGREAVRQMIDELAAKRID
                     TLTLEKSFLLPKVFELVYAPIWVAHYEYQGGDYTAVLDAVRGEILGGTAPINLTARTR
                     MMILSLAAGGIMIGSSLAMLLHAGAHPLSELFQVILLLLGVAMTMAAYPAFKEGRTFV
                     STGTMKHISSLRPAVRIPKKLTDHEILKRDSTILLCPVCGEEVEQPWGEVISVCKKCH
                     RLLNVTSNEVNVVPYDVAKPDMLAQAALESEPEYIPFWMFEVENKVTDLLRTGDTETG
                     LPEISGRRNYYICAADIPRYLAERWEIDLTIRNPEFSELPLNEHVEMKPAILNQNTAK
                     ELSEFLFLRYEEEKPGILQVLRYDFAVKSSRIVYVPYYVEDKTYIPGV"
     gene            complement(1959..>2182)
                     /locus_tag="O0X12_01900"
     CDS             complement(1959..>2182)
                     /locus_tag="O0X12_01900"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834027.1"
                     /note="catalyzes the formation of
                     pyridine-2,3-dicarboxylate and 5-phospho-alpha-D-ribose
                     1-diphosphate from nictinate D-ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylating.nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HJK52946.1"
                     /translation="VDILMFANMTPDAVREAIAVLAGMGLRDRLVLEVSGGITPETIH
                     LWAGVDVDRISMGSLTHSVRCADVSLDIE"
BASE COUNT          549 a          484 c          630 g          519 t
ORIGIN      
        1 atataaaata ctatcatgac accgaggcag ttgatatacc ggaggatgtg actgtgctgc
       61 cggtgaccgt ggactcctcc gtgtatctgc cagaacttca cgggattgca cgcggcgagg
      121 agctgatcgg ggtgccgctg tggttgatca gttacaaggt gaagcataac atctacatga
      181 ttgaggttga ggcacactcc ggtctggttc tgccggagtg gcatccgatc aaagagccgg
      241 tgaactggaa gaagacagcg atgacagcat ttattccgat gtttctgcta agtctcgttg
      301 cgatttactt caatccgtgg atttttgtga ttgtggtaat actactgttt atcttccttt
      361 atcagagtga gatgcttggc ctgattacga tgaaagagag ggaggagccg catgggcctt
      421 gagcgttcag ttgaggatcg cgtcggacgg gtaatgtacc ggtcgaaggt gacgaaaggc
      481 gttgccgacc ggacgatccg cgactggttt actaagggca tcaaagcacc ggatcttcca
      541 aacaaggctg tgatgacgga actgtatatg atctacattc cgttctggag atttgttgcc
      601 caagggaaag cagtcgcctg cggttattcg gagtataatg agaagacagg caatacgata
      661 cgcaacatct acaaagaact gatcgatgac gagtttgtgt ggacggaggt tgcctgtgat
      721 acagggaagt atggccttac aaagctctgg ctggaaccgg gaggggaagt cccctataca
      781 ccgggagcag tagtctcgat ggagccaggg ggatcagcag ttgaggcaag caggcgggga
      841 cgggaagcag tgcgacagat gatcgatgaa ctggcagcaa agcggattga tacgctgaca
      901 ctggaaaagt cgttcctttt gccaaaggtc tttgaactgg tatatgcacc catctgggtt
      961 gcccattacg agtatcaggg cggggattat acggcggttc ttgatgcggt gcgcggagag
     1021 atccttggcg gcacagcacc gatcaatctg actgcaagaa cacggatgat gattctgtcg
     1081 cttgcagcgg gcggtattat gataggatca tcgcttgcaa tgctgctgca tgcaggagca
     1141 cacccactct cggagctgtt tcaggtaata ttactgttgc tcggtgttgc gatgacaatg
     1201 gcggcgtatc ctgcctttaa ggaaggaagg acgtttgtga gtaccggtac aatgaagcat
     1261 atttccagtc tgcggcctgc ggtgcggata ccaaagaagc tgacagatca tgagattctg
     1321 aaacgagact caacgattct tctctgtcct gtatgtggcg aagaggttga gcagccgtgg
     1381 ggtgaagtaa tttcagtctg taaaaagtgt caccgattac tgaatgtgac aagcaacgag
     1441 gtgaacgtgg taccgtatga tgttgcaaag ccggacatgc ttgcacaggc agccctggaa
     1501 agtgaacctg agtatattcc gttctggatg tttgaggtgg agaataaggt gacggatctg
     1561 ctacggaccg gtgatacaga gacagggcta ccggaaattt ccggaagacg gaattactat
     1621 atctgtgcgg cggatattcc ccggtacctt gcggagcggt gggagattga tctgacgatc
     1681 aggaacccgg agttttctga gctgccgctg aatgagcatg tggagatgaa gccggcgatt
     1741 ctgaatcaga atacggcgaa ggagctgtcg gagtttttat tcctccgtta cgaggaggaa
     1801 aaacctggaa tattgcaggt gcttcggtat gattttgcgg tgaagagttc gcggattgtg
     1861 tacgtgccgt actatgttga ggacaaaacg tacattccgg gagtctgaat ctttcctttt
     1921 ttgatgtttg ggatatccag ctataccaca aaccagatct actcaatatc caaactcacg
     1981 tcggcacacc gtaccgaatg cgtcagagac cccatgctga tccgatccac atcaactccc
     2041 gcccacagat gaattgtctc cggcgtaatc cccccggaca cctcaagcac cagccggtca
     2101 cggagaccca ttcctgcaag gaccgcaatc gcctcccgta ccgcatcagg cgtcatatta
     2161 gcaaacatca gaatatcaac tc
//
LOCUS       DALTZZ010000104         5560 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_16265,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000104 DALTZZ010000000
VERSION     DALTZZ010000104.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16265"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="O0X12_01905"
     CDS             complement(<1..648)
                     /locus_tag="O0X12_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HJK52947.1"
                     /translation="MPETLTPSQKRALDVTKSTCVTAGAGTGKTFILSQRYRSVLLQN
                     HANKQFSPANILALTFTEKAAAEMRERIAADLSEDIRACNDPTVQQGLLRMQDEFYRA
                     SVTTFHSFASSILREFAVEAGLDPSFGIMDAGSQSDLVHTTVTALLTHPPEDLRQDVL
                     CLLQYLKSDTLSSLLAYALDDWRKAEPWFQQLEENPDAVRELWETTSADFLVLVQD"
     gene            complement(648..3593)
                     /locus_tag="O0X12_01910"
     CDS             complement(648..3593)
                     /locus_tag="O0X12_01910"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HJK52948.1"
                     /translation="MSVSHTIRYALFGELLTETVAAYQDAASSDPFGTWFVLPTRNLA
                     DVILRHPIAKERLILPDHMLTARDFAQYICRETDPDLYILSSGEQDLLIRRILTSPDL
                     RKSTASLFGEVTHTTCSSLRTLITGLEQNLTDLSVIHSSTRKIETLSAIHQEYTRILK
                     EEHLADKETVYETAVVALGSGKIPFRTVFIYGMYSPQPNEQAFINALIAAAESVHEVR
                     PAVNNPAIFPGFTGKPIHGDPAKAAFAEGLFAGHEHRIPASLPVKLTTVIYPDTTAEV
                     SSVLDQVSGLFEDGVSAEDILILSPAVRETVRLIDDIIDDFSIRNPDGTRTRISYTAA
                     TARPISNYPETTALLAVLKAPVADYPLATLLQLLTLPQIRKKFGIHTSQLFSLSQTLG
                     ITRGKSNWITIADQRIPFLLQKIEDAGTDGQSAKFYQVQIDELQKLQQGLISLIEEIT
                     PKNQKKQSVRQHCRDSLDLFIKLGLLEPAECEMPTEQQLRQKAFHDLISGLAASPVAQ
                     TISLTHAEFCDTLSELCSSTPAPLSPRTAKHTITVAGLRESALTSANHVFILGLSDKS
                     IPRIQFTLPYLTVKETAKIKNTSFETDILREKYYFAAACLVAGESLHLSCAERDSSGA
                     LSPSSFFTRFPEQYKTQAPDFLSHSQTDNQMTAGLAVLNNTLADHPDLFGLPPYPPRK
                     QETLQVCFGDLPDLAERFSSAYNDQTTFAPTMLETYASCPFKWYLEKHLRLIPPVSSH
                     SEERSMLGLVLHRIMERFFTEWTDPITLENKEDAKLCLTEIAAEEFDRPGFTSPEWEA
                     EKNLYLTLRLFGVIDKELDPKLIHQPAAFRRLEQPLADTTAVSVPDAAPLRISGRPDR
                     IDFFPDNSFTVYDYKSGNIEKKNKYEVKGLKDGISLQLPLYVAAVEAKDPNRSGDAAF
                     YQIHPRGVKLRRFSKDVLYKHLPDILSYCKSYRSGMQNGVCSPNPNSSDCSYCPWRFV
                     CRVTTGGEE"
     gene            3818..5524
                     /locus_tag="O0X12_01915"
     CDS             3818..5524
                     /locus_tag="O0X12_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HJK52949.1"
                     /translation="MTETASHNMTDYEETYSTFSIAVPEYYNFGFDVVDAWAKKDRNK
                     LAMIWTNQKGEEKYFTFRHMMNLSNQIANMMFKQNIGKGDRVMIMLPRVPEWWTFAIA
                     AIKIGAVFCPSPCILTPHDLKYRINQGKFKMIVTDMENSWKIEEILSECPTLQIKFLT
                     DGGLPGWINYQTELVHPAPASTKLIPLARSVRTKATDPMLIFFSSGTTGDPKMVLHTH
                     AYPLGHIVTGRFWYDLTENDLHFTVADTGWGKSSWGKFYGPWMQGACVFVYDYRGKFN
                     ATELLPLIEHYEITTFCAPPTIYRMLILADLKAFDFSELRHCVSAGEALNPEVTRVWE
                     EATGKTIYEAYGQTETVMVIGTFPCIPNKPGSIGKPAPGWCVQLHDEQGNEVPVGEEG
                     KIAIKTKDPSPVGLFKEYLDNPEATSAVFVNGWYYTGDKAVKDEDGYFWFMGRDDDII
                     KSSGYRIGPSEVESALIEHPAVKESAVVGSPDPIRGVIVKAFVILKDGWKPSDELVKE
                     LQNHVKKTTAPYKYPRAIEFVEDLPKTISGKVRRVELRDREMKRYQALHHNDLESDSG
                     SQ"
BASE COUNT         1404 a         1233 c         1593 g         1330 t
ORIGIN      
        1 atcctggact aacaccaaga aatctgcact agttgtctcc cagagttccc ggacggcatc
       61 agggttctcc tccagctgct ggaaccaggg ttccgctttc cgccagtcat ccaatgcata
      121 tgcaaggaga gaactgagag tatcgctctt cagatactgc agaagacaga gaacgtcctg
      181 ccggagatcc tctggcggat gagtgagaag agcagttact gttgtatgaa caagatccga
      241 ttgtgatcct gcatccatga taccaaacga cggatcaaga cctgcttcga cggcaaattc
      301 cctgagaatg gaggatgcaa aactgtggaa ggtggtgacg gatgcacggt agaactcatc
      361 ctgcatccgc aaaaggccct gctgcacagt tggatcattg cacgcacgga tatcttccga
      421 aagatcggct gcgatgcgtt cccgcatctc ggctgcggct ttctcggtaa aggtaagagc
      481 aaggatattt gccggactga actgtttgtt tgcatgattc tgcaaaagaa cagaacgata
      541 gcgctggcta aggatgaagg tcttaccggt acctgctccg gcagtgacac aagtactttt
      601 ggtaacatca agtgcccgct tctgagaagg ggttagtgtt tcgggcatca ctcctcacct
      661 cccgtagtta cgcggcagac aaatcgccat ggacagtatg aacagtcaga actgttcgga
      721 tttggagaac aaacgccgtt ctgcatcccg gaccggtagg atttgcagta ggagaggata
      781 tccgggaggt gcttgtaaag tacatctttc gaaaacctcc gcagttttac cccgcgtgga
      841 tggatctgat aaaatgcggc atcaccagat ctgttgggat cttttgcctc aaccgcagca
      901 acgtagagcg gaagctgaag cgagatgccg tctttgaggc ctttgacttc gtacttattc
      961 tttttttcga tattacctga tttgtagtca tagacggtga agctgttgtc agggaaaaaa
     1021 tcgatgcggt caggccgtcc tgaaatacgc agaggggcgg catccggcac agatactgca
     1081 gtggtgtctg caagaggttg ttcaagacgg cggaatgcgg ccggttggtg aataagtttc
     1141 ggatctagtt ccttatctat tacgccaaag agccggaggg tgaggtagag attcttttca
     1201 gcctcccact caggactggt aaagccagga cgatcaaact cctcggctgc gatctctgta
     1261 aggcatagct ttgcatcttc tttattttcc agggtgatcg gatctgtcca ttcagtaaag
     1321 aaccgttcca tgattcgatg aagaacaaga ccaagcatgc ttctttcctc cgagtgagaa
     1381 gacaccggcg gaatgagacg gaggtgtttt tcgaggtacc atttaaacgg acaggaggca
     1441 taggtctcaa gcatggtagg agcaaaggtg gtctgatcat tgtaggcaga agaaaaccgt
     1501 tcggcaagat caggaagatc accaaaacaa acctgcaagg tctcctgttt ccgcggagga
     1561 tacggaggga gaccaaatag atcaggatgg tcagcgaggg tattgttcaa aacagcaagc
     1621 cctgctgtca tctgattatc agtttgggaa tgggaaagga aatctggtgc ttgggttttg
     1681 tactgttccg ggaaccgggt aaaaaatgag gaagggctaa gggccccgga ggagtcgcgt
     1741 tcagcacagg agagatgcag gctctctcca gctacaaggc aggctgcggc aaagtagtac
     1801 ttttcacgga ggatatcggt ttcaaaggag gtatttttga tttttgcagt ctctttgacg
     1861 gtcagatacg gaagagtaaa ttgaatgcga gggatggact tgtcggaaag accgagaata
     1921 aagacgtgat ttgcagaggt gagggccgac tctctgagtc cggcaacggt gatggtgtgt
     1981 ttagcggtac gaggagagag cggggcagga gtgctgctgc acagttcgga aagtgtgtca
     2041 cagaactcgg catgagtgag actgatggtt tgtgcaaccg gtgatgcagc aagacccgag
     2101 atgagatcat ggaacgcttt ctgcctgagc tgctgttctg ttggcatctc acactcagca
     2161 ggctcaagca gacctagctt aataaaaaga tcaagactgt ccctacagtg ctgcctaaca
     2221 gactgttttt tctgattttt tggagtgatt tcctcaataa gactgataag accctgttgg
     2281 agtttctgaa gttcatctat ctgtacctga tagaatttgg cagactgacc gtccgtgcct
     2341 gcgtcctcga tcttttggag aaggaacgga atccgttggt cagcaatagt gatccagttg
     2401 gattttccgc gagtgatacc gagcgtctgt gagagagaga acagttgtga tgtgtggatg
     2461 ccgaactttt tccggatctg cggaagcgta aggagctgga gaagagtggc caggggatag
     2521 tccgctacag gggctttgag aactgcgaga agggctgtcg tttccggata gttactaatc
     2581 ggccgtgcgg ttgcagcggt gtacgagatg cgtgttctgg taccgtctgg attccggata
     2641 ctaaagtcat caatgatatc atcgatcagc cggacagtct cccttacggc tgggctgagg
     2701 atgaggatat cctctgcact gacaccatcc tcaaaaagac cggatacctg atcaagaacc
     2761 gaggaaactt ctgcagtggt gtccggatag atgacagtgg taagcttgac cggaagggat
     2821 gcaggaatac ggtgttcgtg tccggcaaac agtccttcag caaatgcagc ctttgctggg
     2881 tccccgtgaa ttggttttcc agtaaatccc gggaaaatag cagggttatt cactgcggga
     2941 cgcacttcat gcacactctc agcagcagcg ataagagcgt tgatgaaagc ctgttcgttg
     3001 ggctgcgggg agtacatccc gtagataaag accgtcctga aaggaatttt tcctgaacca
     3061 agggcaacca cggcagtttc gtagacagtc tctttatctg caagatgttc ttcctttagt
     3121 attctggtgt attcttggtg gattgcagag agtgtttcta tctttcgggt tgaagagtga
     3181 ataacagaga gatcggtcag attttgctca agaccggtga tgagtgtccg gagactgctg
     3241 caagtggtgt gggtgacctc gccaaaaaga gaggcggtac ttttgcgaag atcagggctt
     3301 gtgagtattc tgcggatgag aaggtcctgt tctccagagc taagaatgta gagatccgga
     3361 tctgtttcgc gacagatgta ctgggcaaaa tctcttgcgg taagcatgtg atccgggagg
     3421 atcagtcgtt ctttggctat tggatggcgg aggatcacat cagcaagatt ccgcgtggga
     3481 agaacaaacc atgtaccgaa cgggtcactg gacgcagcgt cctgataggc agcaacggtt
     3541 tcagtaagaa gctctccgaa aagagcgtaa cggatagtgt gtgatacaga cattggtgtg
     3601 aaagatgatg gatattcagg aggttggcgg actataacag tccatttgtg tagttaccgg
     3661 tacgtttcac taactgattt gactcttgtt atttatattg tacttctata tggtagtttg
     3721 cctggggaca ggagagccag caccaccaga ataccgggca ggatcaccta tccatcacga
     3781 gagaatttat catctgtcgc gtgcaacatg tgagcgcatg accgaaacgg catcacacaa
     3841 tatgacagat tacgaggaaa cctattccac gttttctatt gccgtgccgg agtactacaa
     3901 cttcggattc gacgtagtcg atgcatgggc gaagaaagac agaaacaagc tcgccatgat
     3961 ctggacgaat cagaaagggg aggagaagta ctttaccttc cgccacatga tgaatctgtc
     4021 caatcagatt gcaaatatga tgttcaagca gaacatcggc aagggcgacc gggtaatgat
     4081 tatgctaccg cgggttccag agtggtggac ctttgcgatt gccgccatta agattggtgc
     4141 agttttctgt ccgtccccct gcattcttac tccgcacgat ctgaagtacc gtattaacca
     4201 gggcaagttc aagatgatcg taacggacat ggagaactcc tggaagattg aggagatcct
     4261 gtccgagtgt ccgactctgc agattaaatt cctgacagac ggtggactgc ccggctggat
     4321 taattaccag acagaactcg ttcacccggc tcctgcgtca acgaagctga ttccgctggc
     4381 acgcagtgtc cgcacaaagg caaccgatcc gatgctgatc ttcttctcgt cgggaacgac
     4441 cggtgatcca aagatggtgc tgcatacgca cgcttatcct ctgggacaca ttgtaaccgg
     4501 aaggttctgg tatgatctga cggaaaatga tctgcatttc acggtcgcag acaccggatg
     4561 gggaaagtcc tcctggggta agttctacgg cccctggatg cagggtgcct gtgtgtttgt
     4621 gtacgattac cgcggaaagt tcaatgcaac cgaacttctg ccgctgattg aacactacga
     4681 gatcacgacg ttctgtgctc cgccgacgat ttaccggatg ctgattctgg cggatctgaa
     4741 ggcgtttgac ttctcggaac tgcggcactg tgtgtctgcc ggtgaggcac tgaacccgga
     4801 ggttacccgt gtctgggaag aggcaaccgg aaagacgatc tacgaggcat acggccagac
     4861 tgagactgtg atggtcattg gtaccttccc ctgtattcca aataagccgg gatccatcgg
     4921 caagcccgct ccaggatggt gcgtgcagct gcatgatgag cagggcaatg aggtgccggt
     4981 cggtgaggaa ggtaagattg cgatcaagac aaaggatcca agtccggtcg gtctctttaa
     5041 ggagtatctg gacaaccccg aggcaacgtc ggcggtgttt gtaaacggct ggtactacac
     5101 gggagataaa gcggtaaagg acgaggacgg ctacttctgg tttatgggcc gtgatgatga
     5161 tatcatcaag agttccggct accggattgg tccaagcgaa gtggagtcgg cactgatcga
     5221 gcacccggcg gtgaaagagt cagcggttgt gggaagtcca gatccgatcc gcggcgtgat
     5281 tgtgaaagcc tttgtgattc tgaaggatgg ttggaagccg tcggatgagc ttgtcaaaga
     5341 gttgcagaat catgtgaaga agacgaccgc accgtacaag tacccgcgtg cgatcgagtt
     5401 tgtggaggat ctaccgaaga cgatctccgg caaggtccgg agagttgagc tccgcgaccg
     5461 cgagatgaag cggtaccagg ctctgcacca caatgatctg gagtcagact ccggatccca
     5521 gtaacttttt ctgtgtgtta aatagaacag gcacaatcca
//
LOCUS       DALTZZ010000105         1529 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_16524,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000105 DALTZZ010000000
VERSION     DALTZZ010000105.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16524"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /gene="sepF"
                     /locus_tag="O0X12_01920"
     CDS             complement(<1..368)
                     /gene="sepF"
                     /locus_tag="O0X12_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HJK52950.1"
                     /translation="MGFLDGVFGSKKDTLTEDEYMKLDLASYEGVAGAEEPAVMYVKV
                     ASIADIKDCPRVKDEVYNNNIVIVDITKLKLDKIMFDRVMGDLRAVSKDVNGDIIGLG
                     DQRYVIITPTGVRISREKIGG"
     gene            complement(420..917)
                     /locus_tag="O0X12_01925"
     CDS             complement(420..917)
                     /locus_tag="O0X12_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HJK52951.1"
                     /translation="MVELTIKKRHAIKKSQLASLMQKLTDTIGSDAALYNAPMIEIAE
                     TASKFNIYIIDKKPLIMEQDTWAFPTLRGAVLRPFSGHRIVVDMGAVPYMINGADVMR
                     PGIVSVTDDVVAGKPALVVDESHDKPLAVVIPLYDAAGIIALEKGKAAKNMHYIGDEL
                     WNLEL"
     gene            1178..>1529
                     /locus_tag="O0X12_01930"
     CDS             1178..>1529
                     /locus_tag="O0X12_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HJK52952.1"
                     /translation="MKILKSDDPKISLIRDILSVVIVVAIIGGVLFGVSGTWPAIVAV
                     ESGSMVPNMNVGDLIFVVEENRFGPLLTAEEAEAAGVTSFNGYGDVIIYRPNGNTQVT
                     PIIHRALTEINASTA"
BASE COUNT          412 a          351 c          375 g          391 t
ORIGIN      
        1 cccccaatct tctcacggga gatgcggaca ccggtcggtg tgatgataac gtaccgctga
       61 tctccaagac caatgatgtc accattcaca tctttggaaa cagcccggag atcgcccatc
      121 acgcggtcaa acatgatctt gtcaagcttg agtttggtaa tatcaacgat cacaatattg
      181 ttattataga cttcatcctt aacacgcggg cagtccttaa tgtcggcaat acttgcgacc
      241 ttcacataca tgaccgccgg ttcctcggca cctgcaactc cttcgtagga ggcaaggtca
      301 agtttcatgt attcatcttc agtaagggtg tccttttttg atccgaaaac tccgtcgaga
      361 aatcccatat aaaaaatagt taatttgttg gttatttaat gctttggtat gatcacagat
      421 cagagttcaa gattccagag ctcgtcaccg atgtagtgca tattctttgc ggcctttcct
      481 ttttccagcg cgatgatgcc ggcagcatca tacagaggaa taacaacggc aagcggttta
      541 tcgtggcttt cgtccacgac aagggcaggt tttcctgcaa caacatcatc agtcacagag
      601 acaattccgg gccgcattac atctgcgccg ttgatcatgt agggaactgc gcccatatcc
      661 acaacgatcc gatgaccgga gaaggggcga aggacagcac cccgaagtgt cgggaaggcc
      721 caagtgtcct gttccatgat aagcggtttt ttgtcgatga tatagatgtt gaactttgag
      781 gcagtctcgg caatctcaat catcggggca ttgtaaagtg cagcatcaga accgatggta
      841 tcggtaagtt tctgcatgag agatgccagc tgactttttt tgattgcgtg acgctttttg
      901 atcgtgagtt ctaccatttg tgcatactcc gttggttaca ggaaactata atggttcagt
      961 ttgccaggca aaaggtccct tgagaaaaac agaaaaaaac agatcccaaa gacagaacaa
     1021 ggacaggtat gatccagtac tgaggattga cgaacaacag ggtattacgg gaaacttcta
     1081 taccagtgag gacttagtta gatacagcat tatgtcgaag tcgcccaaaa aagcagaaag
     1141 taatgcagac gcggacgatg tccaaaaatc ctctcttatg aagattctga aatcggatga
     1201 tccaaagatc agtcttatcc gtgatattct ctcggtggtc atcgttgttg ccataatcgg
     1261 cggtgtgctg ttcggtgtgt cgggaacctg gcctgcgatc gttgcagttg agtcagggag
     1321 tatggtgccg aacatgaatg tcggagatct gatctttgtg gtcgaagaga accggttcgg
     1381 acctcttctg actgcagaag aggctgaagc cgccggagta acatctttta acggatacgg
     1441 cgatgtgatc atctaccggc cgaacgggaa tacacaggtg acgccgatca ttcaccgggc
     1501 acttacagag atcaatgcat caaccgcag
//
LOCUS       DALTZZ010000106         2634 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_16663,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000106 DALTZZ010000000
VERSION     DALTZZ010000106.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16663"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="O0X12_01935"
     CDS             <1..582
                     /locus_tag="O0X12_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HJK52953.1"
                     /translation="DKTREIAEKYADKVFIQTSKKVGGARNDGAMAAKYDILATTDAD
                     CFLPRDWLEKILADFAKYPDASTIYGIVYPLEPGIKHKLSLLGYNIVSRIGYWTGTIY
                     MTLGCNTAFRKDLFVGAGMYITADAGDDFEIARRMKKYGRVRLDTNLKVGFSMRRYIE
                     FGAIKSIYQWLYIVAHGGSSEKYQYAGKEYGKK"
     gene            complement(590..1243)
                     /locus_tag="O0X12_01940"
     CDS             complement(590..1243)
                     /locus_tag="O0X12_01940"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HJK52954.1"
                     /translation="MITASPKSILKRTILLCIALGVMALGVALSIKADLGTSPISSVP
                     YVVSLISAMSVGTATILVNAILLVGEILIMRRQFQPIQLLQLAVVTLFGWLIDLCLWG
                     LDGLTYADYSQQWLICIAGIIILGIGVSLEVTANLIPLPGEGLILSICTVLPIKFGNM
                     KVITDVSLVVAAVLLSFIFLADLYGVREGTIAAAICVGLVSKMVIRLIGPYQRWLTS"
     gene            complement(1394..2539)
                     /locus_tag="O0X12_01945"
     CDS             complement(1394..2539)
                     /locus_tag="O0X12_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HJK52955.1"
                     /translation="MTVVGAACCGSYDRDAVLTAVEKAVNAAGGLPPVRGKRVLIKPN
                     LLSDLGADSAVTTHPEIVRAVCRMVLDAGGQTVIADSPGAGHLYTPKTLRRIYERSGM
                     AAVADETGAELSFDTGSAEVPYPDGTVMKRFTVITPAQNADVIISVCKLKTHMFTKFS
                     GAVKNTFGVVPGLDKPVFHSRFVDQQDFSEMLVDLNELIGVDFVIMDAVVGMEGNGPM
                     GGDPKFCGYVLASNSVYGLDYSAQRLIGMEPDQIATTAAARRRGLLDMVEERGDTIVP
                     VQNFVLPAIYAGPQRNTRLRRMILSQMQKAGRVYAPAPRVIQELCIGCGQCVRICPKK
                     AVRLTNGKAVFDLSRCIRCYCCHEMCQARAIDLKRGVVGELLHTLIK"
BASE COUNT          659 a          703 c          663 g          609 t
ORIGIN      
        1 gacaaaaccc gtgagattgc ggaaaagtac gcggacaagg tgtttattca gacgagcaag
       61 aaggtcggtg gtgcccgcaa cgacggtgcg atggctgcga agtatgatat ccttgcaacc
      121 accgatgcag actgcttcct tccccgtgac tggctggaaa aaattcttgc tgactttgcc
      181 aagtatccgg atgcatcaac gatttacggc attgtgtatc cgcttgagcc ggggatcaaa
      241 cacaagcttt cgctgctcgg ctacaacatc gtgtcccgta tcggctactg gacgggaacg
      301 atctatatga cgcttggatg caacacggcg tttcggaagg atcttttcgt gggggccggg
      361 atgtacatta cagcggatgc gggagatgac tttgagattg cacgccggat gaaaaagtac
      421 ggacgtgtca ggctggatac aaatctgaag gtcgggttct cgatgcggcg gtacattgag
      481 ttcggggcga taaagtcgat ttatcagtgg ctgtatattg tggcgcacgg aggatcttcg
      541 gagaagtacc agtacgccgg aaaggaatac gggaaaaaat aatctttttt tagctggtca
      601 gccatctctg gtatggtccg atcagtctga tgaccatttt tgaaacaagg ccgacacaga
      661 tcgcagctgc gatggttcct tcccggacac cgtagagatc ggcgaggaag ataaatgaga
      721 gcagtacggc agcgaccaca agggagacat cggtgattac tttcatgttg ccgaatttga
      781 tcgggagaac agtgcagatg gagaggataa gcccttcacc tgggagcggg atgaggtttg
      841 cggtgacctc aaggcttacg ccgattccga gaatgatgat acctgcaata cagataagcc
      901 actgctggct gtagtctgcg taggtcaggc cgtcaagtcc ccagagacac agatcaataa
      961 gccagccgaa gagtgtgact accgcaagtt gtaagagctg gattggctga aactgccgtc
     1021 gcatgatgag gatctcaccg acaagcagaa tggcattgac gagaatggtt gcggtaccta
     1081 cgctcatcgc agatatgagg ctgacaacgt agggaacact ggagatggga gaggttccaa
     1141 ggtctgcttt gatggaaagg gcaacaccca gagccatgac gcccaaggct atgcagagaa
     1201 gtatagttcg tttgaggatt gattttggtg aggcagtgat cattgaggat ttcgcccgct
     1261 gtgtgataaa tatatttgtg gtttggggat ttacgtgttg tttctccaaa acacagattt
     1321 cctgcggaaa tgtatggtct ctgccccaaa gaccgaacat cccacaacac atgtgatttc
     1381 ccggtgcttg tcttcactta attagagtgt gaagcagttc cccaacaaca ccccgtttca
     1441 gatcaattgc ccgtgcctga cacatctcat gacagcagta gcagcggata cagcgggaaa
     1501 gatcaaacac agcttttccg tttgtaagcc gaaccgcctt tttcgggcag atacgaacgc
     1561 actgcccgca cccgatgcat agttcctgta tcacacgcgg tgccggtgca tatactcttc
     1621 ctgccttctg catctggcta agaatcatcc tgcggagccg ggtattcctc tgtggtccgg
     1681 catatattgc aggcagaaca aaattctgta ccggcacaat cgtgtctcct ctctcctcca
     1741 ccatatccag cagaccacgc ctgcgtgccg cagcagttgt tgcaatctga tccggctcca
     1801 tcccgatcag ccgctgtgca gaatagtcca gtccgtacac cgaattagat gccagtacat
     1861 acccgcagaa ctttggatca ccccccatcg gtccgttccc ttccattccg accaccgcgt
     1921 ccattatcac aaaatccaca ccgatcagct cgtttaaatc caccagcatc tccgaaaaat
     1981 cctgttgatc cacgaaccgc gagtgaaaca ccggcttgtc cagacctggt accaccccga
     2041 atgtgttctt cactgcgccg gagaactttg taaacatatg cgtcttcagc ttacaaacgg
     2101 agataatcac atccgcattc tgggcagggg taataacggt aaaccgtttc attactgtac
     2161 cgtccggata cgggacctcc gcagatccag tgtcaaacga aagttctgct cctgtctcgt
     2221 ctgccactgc cgccatcccg gaccgttcat aaatacgccg gagggtcttt ggcgtgtaca
     2281 agtgtcccgc tcccggactg tctgcaataa ctgtttgtcc ccctgcatcc agaaccatcc
     2341 ggcacactgc acgcacaatc tcgggatgtg tcgtcaccgc cgagtcagct cccagatccg
     2401 acaacaggtt cggtttgatc aaaacacgtt ttccccgaac cggcggaagc cctcctgcgg
     2461 catttactgc tttttcaacc gctgtcaaaa ccgcatcacg atcgtacgaa ccacagcacg
     2521 ccgcaccaac aaccgtcatt gcagttctcc ccgtttttgt tcacgtacca ctaccgacag
     2581 aaccactgca agtactccaa gcactatccc tgaaagaatt gtgaaatgga atcc
//
LOCUS       DALTZZ010000107         1692 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_16689,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000107 DALTZZ010000000
VERSION     DALTZZ010000107.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16689"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            149..976
                     /gene="dapF"
                     /locus_tag="O0X12_01950"
     CDS             149..976
                     /gene="dapF"
                     /locus_tag="O0X12_01950"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HJK52956.1"
                     /translation="MTIAFTKLHGNGNDFILIDEMAGTVIPDDMKAQFAVSYCDRRFG
                     IGGDGVLFISPSDIADVRMRLFQPDASEAEMCGNGIRCLAKYAFDNNYTKTKLFKVET
                     LAGVLTVRAGYDNEGNFSATIDMGVPAYDAASIPATGSGDFVAEIEGMTVYAANTGVP
                     HAVIFVDDVEALDLDAVAPKIRHNSVFPKGTNVNFVQVTGPSAITIRTFERGVEGETL
                     SCGTGSTASALISAKIGKVSGDVVHVDTVGGPLDIGVGERATMTGPAETVFVGEIPF"
     gene            complement(1056..>1692)
                     /locus_tag="O0X12_01955"
     CDS             complement(1056..>1692)
                     /locus_tag="O0X12_01955"
                     /inference="COORDINATES: protein motif:HMM:NF012998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52957.1"
                     /translation="LITQCNLYVVGEHIIRVRHNLLGVPGASTENVREVYSHPQAISQ
                     CRDFLRHHSEINAYPYANTALAAKFVAETHDPAKASIGSLRAAELYGLDILEKNIQTA
                     KNNYTRFVILSRHLEITRQSDKISLVFSTAHKSGALYNVLAHFAYNGLNLLKIQSRPK
                     RGTPWEYIFFLDVAGNLEDANVLIALGKVREQSSWFKLIGNYPHFHHTEKE"
BASE COUNT          378 a          376 c          481 g          457 t
ORIGIN      
        1 gcgcgtactc cgccgtgttc cggtcattga tgagaatctg cctcttttca agacaccaga
       61 gggtgtatcc tggcttgcgg cccgtctcac atccgccggt tctgatgtgt gatcttcaag
      121 ttttctgaag agcaatactg tgagtacaat gacgatcgca tttacgaagc ttcacggcaa
      181 cgggaatgat ttcattctga tcgatgagat ggcaggcacg gttatcccgg atgatatgaa
      241 agcgcagttt gcagtgagct actgtgaccg caggtttggt atcggtggtg acggtgttct
      301 gttcatctct ccgtcggata tcgcggatgt acgtatgcgc ctgttccagc cggatgcaag
      361 cgaagcggag atgtgcggca atggtatccg gtgtctggca aagtatgcgt ttgacaacaa
      421 ctatacaaag acgaaactgt tcaaagtaga aacgcttgca ggtgttctga ctgttcgtgc
      481 cggatatgat aacgagggca acttctctgc cacgattgac atgggtgtcc ctgcctacga
      541 tgcagcatcg atccctgcaa ccggctctgg tgatttcgtt gccgagattg aaggaatgac
      601 tgtgtatgct gcaaacacag gtgttccgca tgcggtcatt tttgtggacg atgttgaggc
      661 gcttgacctt gatgcagttg cgccaaagat tcgccataac tccgtattcc cgaagggaac
      721 aaatgtgaat tttgtacagg tgactgggcc ttctgcaatt acgatccgga catttgagcg
      781 gggagttgag ggagaaacac tctcgtgcgg taccggttca accgcatccg ccctcatctc
      841 tgcaaagatt ggaaaagttt cgggagatgt ggtgcatgtt gacacagttg gcggccccct
      901 ggacatcggt gtcggtgagc gtgcaactat gaccggacct gctgaaacgg tatttgtcgg
      961 tgagatcccg ttctaacttt tttttgaaaa aattattttt tggatgggtc gttgccaaac
     1021 tgcatgatta gagcgtatgc agcgcagata ttgcatcact ctttttctgt gtgatggaag
     1081 tgaggatagt ttccgatcag tttgaaccag ctgctctgtt cgcggacttt tccaagagca
     1141 atcaatacgt ttgcgtcctc aagatttcct gcaacatcaa ggaagaagat gtactcccat
     1201 ggtgtacctc gtttgggacg ggactggatc tttaacaggt tcagcccgtt gtaggcaaag
     1261 tgagcaagta cattgtagag tgccccgctt ttgtgggctg tggagaagac aaggctgatt
     1321 ttatctgact gtctggtaat ctccagatgc cgcgaaagga tgacaaatcg cgtgtagttg
     1381 ttctttgcag tctggatatt tttttccaga atgtcaagac cgtagagttc tgcggcgcgc
     1441 aggctgccga tggaagcctt tgcgggatcg tgtgtctctg caacaaactt tgcggcgagt
     1501 gctgtgttcg catacgggta ggcgttgatc tcactgtgat gccggaggaa gtcgcggcac
     1561 tggctgatgg cctgcggatg ggagtacacc tcgcgaacgt tttcggttga tgcacccggg
     1621 acaccaagca gattatgacg aacgcggatg atgtgttcgc cgaccacata cagattgcac
     1681 tgtgtgatca ga
//
LOCUS       DALTZZ010000108         2412 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_17082,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000108 DALTZZ010000000
VERSION     DALTZZ010000108.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17082"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            85..357
                     /locus_tag="O0X12_01960"
     CDS             85..357
                     /locus_tag="O0X12_01960"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HJK52958.1"
                     /translation="MKLKIIELEKGKIRLSLEGEGHTFMNALTEEILIDPEVDVAKYV
                     IEFQFSDPELFITMKEGSKKDPVTVIREACDRISTRCDDLLGCLKN"
     gene            432..1154
                     /locus_tag="O0X12_01965"
     CDS             432..1154
                     /locus_tag="O0X12_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJK52959.1"
                     /translation="MLDEIRYARSGDAKIAYRITGSGMPLVIVSGLGDSMKDWKESII
                     RTLAERFCVILPDNRGLGLTGMGSIPPQKMTIIQYAEDIHAVVEAEGLSEIFLLGHSM
                     GGMIAQEFAVLYPEMVKKLMLFATDYGPESSYRAHLMNRCVLPLQALCVVAPWHTKGF
                     RAGACAIASWPGSADRLGDIRCRTFLLFGDADFLMHPDVAHEMERMIPDAELKMIPGG
                     THRMHDLYPEEFANTVLEFFGT"
     gene            complement(1201..1815)
                     /locus_tag="O0X12_01970"
     CDS             complement(1201..1815)
                     /locus_tag="O0X12_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HJK52960.1"
                     /translation="MVDSYEDMLKAAYSGMTEPTSTGERFVMPKTKVYIEGKTTVLEN
                     FADIAGTLNRDKAHFRKFILGELGTAGKIDGTRGVFNGKFEESQFEAIVTTYVNDYVI
                     CSECGRPDTKLVKDDRVLMLLCEACGSKRPIRKRKTKTEVQGPAIEEGKELEVQIESI
                     SKKGDGVAHVGKYILYVSGAKAGQTVKVRITRISGSVAFTQKIL"
     gene            1912..>2412
                     /locus_tag="O0X12_01975"
     CDS             1912..>2412
                     /locus_tag="O0X12_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HJK52961.1"
                     /translation="MLAEHDEEYIRSVLGRNLKPLEAAAFEKVWSEDCSYRITRKLLD
                     TLPTIGGSVILGPGDDAAIVKFSDELSLAIAMGSQNRSSCTNPYQGAATGVGSVVRDV
                     LSMGAQPIGIQTPWFFGDISDEKTQWIIRNATAGAADYSNTINVPVLPGYQMFDESFR
                     GNPLINM"
BASE COUNT          587 a          520 c          670 g          635 t
ORIGIN      
        1 atataatact tatccaagat cggatcgttt gatgacgtat cctgcgtcat ctatatagtc
       61 tcatccgcca aataggtaat ccgcatgaag ctgaagatca tcgagcttga gaaaggcaag
      121 atacggctga gccttgaagg ggaaggacac acatttatga atgcactgac cgaggaaatt
      181 cttatagacc ccgaggttga tgtggcaaag tatgtgattg agttccagtt ctccgatccg
      241 gagctcttta ttacgatgaa ggaagggtca aagaaggatc cggttacggt tattcgtgag
      301 gcatgtgacc gcatctcgac gagatgtgat gatctgcttg gatgtctgaa aaactgaaca
      361 aacaggatat gaccagttcc ccagtgatgg catgtttatg ggggacaaaa ccaataatac
      421 ttttttgaac catgcttgat gagatacggt atgcgagatc aggcgatgca aaaattgcat
      481 accggattac cggttccgga atgcctcttg tgatcgtcag tggtcttggc gactcgatga
      541 aggactggaa ggagtcgatc atccggacac tggcagagag gttttgtgtg attctgccgg
      601 acaaccgtgg tcttggtctg accgggatgg gatcgattcc tccccagaag atgacaatta
      661 ttcagtacgc ggaggatatt cacgcggtgg tagaggcaga agggctctcg gagattttcc
      721 tgctcgggca ttccatgggc gggatgattg cccaggagtt tgcggttctg tatccggaaa
      781 tggtaaaaaa gctcatgctg tttgcaacgg attacggacc ggagtcgtca taccgtgcac
      841 atctgatgaa ccgttgtgtg ctcccgctcc aggcattgtg tgtggttgca ccgtggcaca
      901 cgaaagggtt ccgggcaggt gcatgtgcga ttgcgtcgtg gccgggatcg gcagaccggc
      961 ttggtgacat tcgctgccgg acatttttgc tgtttgggga tgcggatttt ctgatgcatc
     1021 ctgatgtggc gcatgagatg gagagaatga tccctgacgc agaactgaag atgattcccg
     1081 gcggaacaca ccggatgcat gatctctatc ccgaagagtt tgcaaatacg gtactggaat
     1141 tttttgggac gtaagagttt ttcaaaaaaa agtgtaccgg gagattccgg aacagatatt
     1201 ttagagaatt ttctgggtga atgcaacgga gccggagatt ctggtgatcc gtactttgac
     1261 ggtctgaccg gcttttgctc cggagacata caggatatat ttgccgacgt gggcaacgcc
     1321 gtctcccttc ttgctgatgg attctatctg aacttcgagt tcttttcctt cctcgatcgc
     1381 cggaccctgg acttcggtct ttgtcttacg ctttcggatc gggcgtttgg atccgcatgc
     1441 ttcgcagaga agcatcagta cacgatcgtc tttgacgagt tttgtatccg ggcggccgca
     1501 ctcggaacag atcacatagt cgtttacgta ggtggtgaca attgcttcaa actgtgactc
     1561 ttcgaacttt ccattgaaga caccgcgggt accgtcgatc ttgccggcgg ttccaagctc
     1621 acctaagatg aacttcctga agtgggcctt gtcacggttg agtgttccgg cgatgtcggc
     1681 gaagttttcc aggacggtgg tcttaccctc aatatagacc ttggtcttgg gcatgacgaa
     1741 ccgttctccg gtgcttgtcg gttccgtcat acctgagtat gccgccttca gcatatcctc
     1801 atatgaatca accatactcc tatattttag cgctggaggc ttaaagttct ggcgtcaccc
     1861 ggatacatac aggtgttttt ctttgcagcg atcaaatagt ttggataagt tatgctggca
     1921 gaacatgatg aggagtatat tcgatccgtt ctcggacgga atctgaaacc gctggaagcg
     1981 gctgcatttg aaaaagtgtg gagtgaagat tgtagctacc ggattacaag gaagcttctg
     2041 gatacactgc cgacaatagg aggaagtgta attctcggac ccggagatga tgcggcgatc
     2101 gtgaagttct cggacgaact ttccctggca attgcaatgg gatcacaaaa ccgttcaagt
     2161 tgtacaaatc catatcaggg ggcagcaaca ggagtgggaa gtgttgttag agatgtgctt
     2221 tcgatggggg cgcagcctat cggaatacag actccctggt tctttggaga catttcagac
     2281 gaaaaaacgc agtggataat tcgtaatgca accgcgggag ctgcggatta cagtaacaca
     2341 atcaatgtgc cggttcttcc gggataccaa atgtttgacg aaagtttcag aggaaacccg
     2401 ctgattaata tg
//
LOCUS       DALTZZ010000109         1994 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_17634,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000109 DALTZZ010000000
VERSION     DALTZZ010000109.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17634"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(110..1732)
                     /locus_tag="O0X12_01980"
     CDS             complement(110..1732)
                     /locus_tag="O0X12_01980"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HJK52962.1"
                     /translation="MDSADRPVYNVGDNVPPSVLVLSILQHFFVLAVYMTYPVIITKA
                     INGGEDLSTFLISATLIGSGIATILQAIRYTGCGYIFPMVPNSSYLPASMLAATAGGL
                     PLLYGMMIVSGFLEMILSRLTRYFRVLFPGEVVGVVMFMLGLAIIPFSFPLFFGSGDT
                     GSLDPAATAVGIITLGSMIILGIQHQKIFRFYAVLIGIIIGFASSVLFGVLTLADIES
                     AGSTSLFSIPNPVGIVSYSFDVGLLIPFAIGMLCVMLKSAGNIALLDEYTGETGRGNL
                     RRGILSEGFGAAVCSAIGGIGIGSSASNTGLIPGTGIASRKIGLGLGLFLIICGFLPA
                     IGWFFHIMPEPIMGAVVIYAIAFIMLGGVQSISSRVLDNRRTFVVILPIMIGVSSVMC
                     PNLYTALPETIQLFFASPLTSGAIFVVVLGLLFKIGLPTHNTIRFGDHPEADVSRLLL
                     DCGRLWTMDKTQMFQIIHHIQALIRAMPEGMHPEQLTLTLKNSTGALTAELIVPGPVD
                     ETAMKSAGHYPATVTIATDAGKTIIRSGYLMI"
     gene            complement(1789..>1994)
                     /locus_tag="O0X12_01985"
     CDS             complement(1789..>1994)
                     /locus_tag="O0X12_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HJK52963.1"
                     /translation="SGPNRFVEFHLMMPRATPLDAAHAVLKEIENELVAEMPRTSVIV
                     HLDPCDGRCGKMECSFTCKKAEK"
BASE COUNT          576 a          464 c          562 g          392 t
ORIGIN      
        1 gcttacatct ccgcaatcat cgtgattgtg atcatcctct acaagatgaa gacccgcaag
       61 ttcaagcggg aagagtaact ctttttttat acagattccg gacattccat cagatcataa
      121 gatagccgga acggatgatc gtttttcctg catcagttgc aatggtaaca gttgcaggat
      181 aatgcccagc agacttcatg gcagtttcat cgaccggacc tggaacgatg agttcggcag
      241 taagggcacc ggtgctgttt ttgagggtga gggttagttg ttcaggatgc ataccctccg
      301 gcatggcacg aataagggcc tggatatgat gaatgatctg aaacatctgg gtcttgtcca
      361 tggtccagag gcgtccgcag tcaagcagga gacgggagac atcagcttcc ggatgatccc
      421 cgaatctgat ggtattgtgt gtcggaagac cgattttaaa gagaagacca agcacaacta
      481 caaagatcgc cccggaggta aggggggatg caaagaaaag ctgaatggtc tcgggaagtg
      541 cggtgtaaag attcgggcac atgacagagg agacgccaat catgattggc agaatcacca
      601 caaaggtccg gcggttgtcc aggacacggg atgagatgct ctgtacacct cccagcataa
      661 tgaaggcgat cgcatagatg acgactgctc ccataatagg ttcgggcatg atgtggaaga
      721 accagccgat tgcaggaaga aatccgcaga tgattaagaa gagaccgaga ccaagaccga
      781 ttttacggga agcaataccg gtcccgggga taagtccggt gttggatgca gacgacccga
      841 ttccaatacc gcctatagca gaacagacag cagccccgaa cccttcggag aggatcccgc
      901 gacggagatt cccacggccg gtttcaccgg tgtactcgtc aagaagtgcg atgtttcccg
      961 cggacttcag catgacacag agcatcccga tggcaaacgg gatgagaaga ccaacatcaa
     1021 aactgtagct gacaattccg acagggttcg gaatggagaa cagagacgtc gatcctgcgg
     1081 actcgatgtc tgcaagcgtg agaaccccaa agagaacaga agaggcaaaa cctatgatga
     1141 tgccgataag aaccgcatag aaccggaata ttttctgatg ctggatccca aggatgatca
     1201 ttgagccgag agtgatgatg ccgaccgcag ttgccgcggg atcgagcgag cctgtatcgc
     1261 cgctgccgaa gaaaagcggg aacgagaacg gaatgattgc aagacccagc atgaacatga
     1321 cgacgccaac aacctcaccg gggaagagca cacggaagta ccgcgtaagc cggctgagaa
     1381 tcatctccag aaatccggaa acgatcatca taccgtaaag gaggggaaga cctcctgcgg
     1441 ttgcggcaag catggatgca ggaagatagg aggagttggg aaccatcggg aagatgtacc
     1501 cgcagccggt atagcggatt gcctgaagaa tcgtagcaat accggaaccg ataagggttg
     1561 cactgataag aaacgtcgag agatcctcgc cgccgttgat tgccttggtg atgatgacag
     1621 ggtatgtcat gtaaacggcg agaacaaaaa aatgctgaag aatactcagg acaagaacgg
     1681 acgggggaac attgtcgccg acattgtaca ccggacggtc tgcagagtcc ataattaaag
     1741 agataggttt gtctggcgga tattagaaag tttggagaga ggcaaaaatt atttttcagc
     1801 cttcttgcac gtaaacgaac actccatctt tccgcagcgt ccgtcgcagg gatccagatg
     1861 cacaatcacc gaagtcctcg gcatctctgc aacaagttcg ttttcaattt ctttgaggac
     1921 tgcgtgggct gcatcaagcg gagttgcacg cggcatcatc agatggaact caacaaaccg
     1981 gttcggtccg gacc
//
LOCUS       DALTZZ010000110         3405 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_17640,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000110 DALTZZ010000000
VERSION     DALTZZ010000110.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17640"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(89..802)
                     /locus_tag="O0X12_01990"
     CDS             complement(89..802)
                     /locus_tag="O0X12_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HJK52964.1"
                     /translation="MKTVLTLLVDCAGVFEGSRTAAANMPVKKFIGCLPPSLPVRILR
                     YSDTAMWHIGPEPVLAGDLEWTDIPSGGYLSSLAHAANLAGQSLQIAPDVQNIVLLIS
                     DGALSDPEEIVQAVLAKRFDPSVIRAAVPLTTDADSAVLRMFAGDNVYDPSVLLDPRP
                     FLSSFGESPAPSVSHPATGQIVLSCTIALDGGDSFSLSVAGMDLDQVKEEMKTSLAGF
                     GTRDDQIRQKIAEYISRVL"
     gene            complement(799..3402)
                     /locus_tag="O0X12_01995"
     CDS             complement(799..3402)
                     /locus_tag="O0X12_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HJK52965.1"
                     /translation="MYWCPHCNVPLVAKTCSCKTETKKIPLQQPYDIRPVLKADHDLL
                     LSLIRDRFGPRVTLPHVMIFNKAGGLDRNDLIIANGVRFAWLWFDPVTHRFRLDIEAE
                     ALPYLVGKADKNIIDLEASASSLPSGRLGGKKIAVTAPDATDGVVILKYKSKYGTGIL
                     KDGSVRIKELVSVQPLLGMANPTWGDVVEKNAFHLKNMERTAVREIKQNLGLAPAANC
                     SFSGGKDSTAVWHIAQKAGVTDAFFIDTGLEFPETIEFVKSQNVRLIQKAGNFWQAVE
                     KAGPPGKDHRWCCKLLKLNPLKVHLAETGPCVTVQGNRWYESWSRASLDAVSRNPNNP
                     LQINLSPLRSWRALDVFLYLWLREVPYNPLYERGYERIGCYLCPAMLESELATMHETH
                     PAMAARWDEFLNRWAADRGLPPEFISWGLWRWKDLPPKMKELVREAGLDLSEKPVKRS
                     TPSSVAHLMPEGKAGDIVDVESSVPAPDWDALRSQFPMIGDLIYLDHAATSFAPECVL
                     AAMDQFERTYRANVGRGVHRLTRIASQKYWHSHEKVSAFIHGEGGTTVFVKNTTEAVT
                     MIARGLAWKPGDVIVTTVLEHHSNLLPWRALEKYGVTLRVVGLRPDLTLDMDAFAAAM
                     DDSVRLVAVTQASNVTGTIVPVAEIAELCRRYGALLAVDAAQSAPHMQIDVRKLGVDF
                     LSFSGHKMCGPTGTGVLWMKEPILEPLFLGGGMVEHVTDDGFTPAEGFHRYEAGTPNI
                     AGGIGLGEAAEFLQEIGMPQIEARERELSCRLLEKLSAIPGVRVYAPSDPVMRIGVVS
                     FTIDGISPHEVAQYLDDEGGILVRSGMHCAEPLMRYLGCPEGTLRASLAFYNTESEID
                     TLAATVREMVS"
BASE COUNT          851 a          919 c          935 g          700 t
ORIGIN      
        1 gaggggttga gtgttctttg ggcaggagta atacaggatt tacacaagat acaggcaccc
       61 aaaaaagtga aaacaaaaag gggaaaggtt acagtacccg cgagatgtac tccgcgattt
      121 tctgtctgat ctgatcgtca cgggtaccaa agcctgcaag agatgtcttc atctcctcct
      181 tgacctgatc aagatccatc ccggcaacag aaagggaaaa cgagtccccg ccgtcaagcg
      241 caatagtaca ggaaagaaca atctgcccag tagccgggtg tgaaaccgac ggagccgggg
      301 actctccaaa tgaggagagg aagggacgcg ggtcaagaag aactgacgga tcatagacgt
      361 tgtctcccgc aaacatacgg aggacggcac tgtccgcatc cgtggtaaga ggaacggcgg
      421 cgcgaatgac ggatggatca aagcgttttg caagcacagc ctgcacaatc tcctcaggat
      481 cggagagggc gccgtcagag atgagaagaa caatattttg tacatcaggc gctatctgaa
      541 gactctgtcc cgcaagattt gccgcatggg caagggaaga gagatatcct cctgacggga
      601 tgtccgtcca ctccagatcc ccagctagaa ccggctcggg accaatgtgc cacatggcag
      661 tgtccgagta acggaggata cggaccggca gggagggagg aaggcatccg atgaattttt
      721 tcaccggcat gtttgcagcg gcggttctgc tgccctcaaa cacaccggca cagtccacaa
      781 gaagggtcag gacggttttc atgaaaccat ctcccgaaca gttgccgcaa gggtgtcaat
      841 ctcactttca gtgttgtaga aagcaagact tgcacggagg gttccttccg gacagccgag
      901 ataccgcatc agcggttcag cacagtgcat tccggaccgc accagaatgc cgccttcgtc
      961 atccaaatac tgtgcaactt catgcgggga tatgccgtca atggtaaagg agacaacacc
     1021 tatgcgcatg acaggatctg acggtgcata gacccggacg ccgggaattg cagagagttt
     1081 ttcgagcagc ctgcaggaga gttcccgttc ccgcgcctcg atctgcggca taccaatctc
     1141 ctgcaagaat tcggcagcct ctccaagacc aatgccgccc gcgatgtttg gtgttcccgc
     1201 ctcgtaccgg tggaaaccct ccgccggtgt gaaaccatca tcagtgacat gctcgaccat
     1261 cccacctcca agaaacagtg gctccaggat aggttccttc atccagagta caccggttcc
     1321 tgtcgggccg cacattttgt gaccggagaa ggagagaaaa tccacaccaa gctttcgaac
     1381 gtcaatctgc atgtgggggg cagactgtgc ggcatcaaca gcaagaagtg caccatacct
     1441 ccggcacagc tctgcaatct ccgcaaccgg gacaatggta ccggtcacat ttgatgcctg
     1501 tgttacggca accagccgga cggagtcatc cattgctgct gcaaacgcat ccatatcaag
     1561 cgtaagatcc ggccggagac caaccacccg cagcgtaaca ccgtactttt ccagtgcacg
     1621 ccagggaagg aggtttgagt ggtgctcaag tactgtggta acaatgacat ccccgggttt
     1681 ccacgcaagt ccgcgggcga tcatcgtgac agcttcagtg gtgtttttca caaataccgt
     1741 ggtgccccct tccccgtgga tgaaggcgga aaccttctcg tgggaatgcc agtacttctg
     1801 gcttgcaatc cgtgtcaggc ggtgaacacc cctgccgaca ttggcccggt atgtccgctc
     1861 aaactgatcc attgcagcaa ggacacattc cggagcaaag gacgttgcag catggtcaag
     1921 ataaatcaga tcgcctatca tcgggaactg actgcgcagc gcatcccagt caggagcagg
     1981 aacagagctt tcgacatcca caatgtctcc tgcttttcct tccggcatga gatgggcaac
     2041 agacgaggga gtactgcgtt tcaccggttt ttcggagaga tcaagacccg cttcacggac
     2101 cagttccttc atcttgggag ggagatcctt ccaccgccac aaaccccagc tgataaactc
     2161 aggcggaagc cctcggtctg ccgcccagcg gttgaggaac tcatcccatc gtgcagccat
     2221 cgcaggatgg gtctcatgca tcgttgcaag ctccgactcc agcattgcag ggcagagata
     2281 gcagccgatg cgttcatacc cgcgttcata cagcggattg tacggcacct cacgcagcca
     2341 gagatagagg aagacatcaa gggcacgcca ggaacgaagt ggagagagat tgatctgcag
     2401 cgggttgttc gggtttcggg agacggcatc aagagaggcg cgactccatg actcatacca
     2461 gcggttcccc tgaacagtga cacagggacc ggtctcagca agatgcacct tcagcggatt
     2521 gagtttgagg agtttacagc accatcggtg atcctttccg ggaggtcctg ctttctcaac
     2581 agcctgccag aagttgccag ccttctgaat aagacggaca ttctgacttt tgacgaactc
     2641 gatggtttcc ggaaactcaa ggccggtatc gatgaaaaac gcatcggtaa ctccggcttt
     2701 ctgtgcaata tgccagacag cggtactgtc tttgccgccg gaaaaggaac agtttgccgc
     2761 aggtgcaaga ccgaggttct gcttgatctc acggaccgcg gtacgttcca tgtttttgag
     2821 atggaaggca ttcttctcca ccacatcccc ccaggtggga tttgccatgc caagcagcgg
     2881 ctgcacacta acaagctctt tgatgcggac ggacccgtcc ttcaggatac cagtgccgta
     2941 cttggatttg tatttgagga taacaactcc gtccgttgca tcaggtgctg tgactgcaat
     3001 ttttttgccg ccaagtctgc ctgaaggtaa ggaggaggca gaggcttcaa gatcgatgat
     3061 gttcttatcg gcttttccga cgagatacgg caaagcctct gcctcaatat caagccggaa
     3121 ccggtgggta accggatcaa accagagcca ggcaaacctg acaccgtttg caatgatgag
     3181 atcattccgg tcaaggccgc ctgccttgtt gaagatcatc acatgcggaa gggtaacgcg
     3241 gggaccaaaa cggtcacgga tcagagagag caggagatcg tgatccgctt taagcacggg
     3301 gcggatatca tagggctgct gaagcggtat ttttttggtc tctgtcttgc acgagcaggt
     3361 ttttgcaaca agcgggacat tgcagtgcgg acaccagtac agata
//
LOCUS       DALTZZ010000111         2612 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_17893,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000111 DALTZZ010000000
VERSION     DALTZZ010000111.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17893"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="O0X12_02000"
     CDS             complement(<1..311)
                     /locus_tag="O0X12_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HJK52966.1"
                     /translation="MNGLLYKDLLNLTPTLKYLVFMALIFCVVFIPLGNELPVYIILI
                     MFGALLPTTAISFDTAARWDKYAVSLPVTRREIVAARYQLMVGSICVAGVISLIIAVA
                     M"
     gene            complement(308..1168)
                     /locus_tag="O0X12_02005"
     CDS             complement(308..1168)
                     /locus_tag="O0X12_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK52967.1"
                     /translation="MEYAINVTGLCKSYQDFALDNVSFAVPKGSIMGFIGENGAGKST
                     TIKAMLNLIHRDAGTVEILGMDLDGHEKEIKERIGVVFDECCFHDNLTPADISKILGN
                     IYQNWDGKLYQKYLVQFGLDEKKKIKEFSRGMKMKLGIAAALAHRPDVLILDEATSGL
                     DPIVREEILDIFLDFIQDENHAILISSHITSDLDKIADYLTFIHHGRIILSDGRDDLM
                     DSMGIVKCGIEDLAKIEKDQIIRYRKNQFGCEVLVRNKRSFAAGHPNMVIDPVTTEAI
                     MLFYSRGEEL"
     gene            complement(1172..1564)
                     /locus_tag="O0X12_02010"
     CDS             complement(1172..1564)
                     /locus_tag="O0X12_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HJK52968.1"
                     /translation="MTQEVLVNIIISNASDKPIYEQITSQIKRMIISGELPEGTALPS
                     MRLLAKELRISVITTKRAYTDLEHDGFIETVPGKGSFVAGSNLEIIKEEQMRLCEEHL
                     QKAVDIATQSGITYEELRESLELLYNGE"
     gene            1703..2020
                     /gene="eif1A"
                     /locus_tag="O0X12_02015"
     CDS             1703..2020
                     /gene="eif1A"
                     /locus_tag="O0X12_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor eIF-1A"
                     /protein_id="HJK52969.1"
                     /translation="MGRIENENQDVAEDGSIIRVKLPNRRAKEQFAQAELMLGSNHIK
                     VRCSDGVTRLGRIKGKIKKRVWIREGDILIVIPWEFQDDKCDIVYRYTTPQVDWLRAH
                     KYL"
     gene            2053..2499
                     /locus_tag="O0X12_02020"
     CDS             2053..2499
                     /locus_tag="O0X12_02020"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HJK52970.1"
                     /translation="MVQIPAFETVAQAHGHTCPGIALGYKIAVVAARWVGDDDDVRIV
                     AHTTRCPLDALKVTFDVRNHPERMVVEDTNTVSFVITKPNGKKLFIDELPGTHLTSDE
                     LTEIKKKVEAGTATADDLRRMNEIKNELFRIMQAIPDEKLFAVREE"
BASE COUNT          731 a          662 c          548 g          671 t
ORIGIN      
        1 atcatagcaa ctgcgatgat aagtgagatg acaccggcaa cacaaatgct gccaaccatc
       61 agctgataac gtgctgccac aatctcccga cgggtcaccg gcagactcac cgcgtactta
      121 tcccaccggg cagctgtatc aaaactgatc gcagttgtcg gcaacagcgc accaaacata
      181 atcaggataa tatacaccgg cagctcattt cccagcggaa taaacaccac gcagaagatc
      241 agtgccatga ataccaggta cttcaacgtc ggtgtcagat tcagcagatc cttgtacagc
      301 agaccgttca cagctcctca ccccgcgagt agaacaacat aatcgcctcg gtcgttaccg
      361 gatcaatcac catgttcgga tgcccggcag caaaggatct cttgttgcgg accaaaacct
      421 cacaaccgaa ctgatttttg cggtagcgga tgatctgatc cttctcgatt tttgccagat
      481 cctcaatgcc gcacttcaca atacccatac tgtccatcaa atcatcccga ccatctgaaa
      541 gaatgatacg gccgtgatga atgaacgtca gatagtctgc gatcttatcg agatcactgg
      601 tgatatggga tgagatcaga atcgcatggt tttcatcctg aatgaaatca aggaagatat
      661 caaggatctc ctcgcggaca attggatcaa gaccacttgt tgcctcatcc agaattaata
      721 catccggcct gtgagcaaga gccgccgcaa tgccgagttt catcttcata ccgcgggaaa
      781 attctttaat tttctttttt tcatcaagac cgaactggac aagatacttt tgatatagtt
      841 tcccgtccca attctgatag atatttccta ggattttgga aatgtcagca ggagtaagat
      901 tgtcgtggaa acagcactca tcaaacacca cgccaattct ctccttaatc tccttttcat
      961 gaccgtcaag atccatgcca agaatctcaa cggtacccgc atcacggtga atgagattca
     1021 gcattgcctt gatcgtcgtt gattttcccg caccgttctc cccgatgaat cccatgatag
     1081 atccttttgg tacggcaaag gaaacattgt ccagtgcaaa atcctgatac gatttgcaaa
     1141 gacctgttac gtttattgca tactccattt cttactctcc attgtaaagc agctcaagag
     1201 actcccgcag ctcctcatac gtgataccgc tttgggttgc gatgtccact gccttttgca
     1261 gatgctcctc acacagtcgc atctgttcct ctttgataat ctcaagattt gatcccgcca
     1321 caaagctgcc tttcccggga actgtttcaa tgaatccgtc atgttccaga tcagtatagg
     1381 cacgttttgt ggtgatgaca ctgatgcgca gttcctttgc aagaagacgc atacttggca
     1441 aggcagttcc ctccggaagt tcgccgctga tgatcatgcg ttttatttgc gaggtaatct
     1501 gctcgtaaat gggtttatca ctggcattgc tgatgatgat gttcacgagg acctcctgcg
     1561 tcacattgta tatattgtat atacacatta atacttttgc atggaagaaa gactcagcat
     1621 ctggagattt tccaaaaacc tatcttcagg catatttatc agatgacaca accaatgtat
     1681 tacccaaaca ggggtagata ttctgggtcg aatagagaac gaaaatcagg acgtagcaga
     1741 agatggaagc attattcgtg taaagcttcc gaatcgtcgt gcaaaagaac agtttgccca
     1801 ggcagagctt atgcttggtt caaaccatat caaagtccgt tgttcagacg gtgtcacacg
     1861 tctcggtcgg attaaaggta agattaagaa acgggtctgg atccgcgaag gagacattct
     1921 cattgttatt ccctgggagt tccaggacga caaatgtgat attgtctacc gctacactac
     1981 cccgcaagtg gactggctcc gcgcacacaa atacctctaa acacatcatc accatttttt
     2041 ctcagaacaa tcatggtaca gattccagca tttgaaacag tagcacaagc tcacggacat
     2101 acctgtcctg gtattgctct tggttacaaa attgccgtcg ttgcagcaag atgggttggt
     2161 gacgacgacg atgtacggat tgtcgcccac actacccggt gcccactgga cgctctcaaa
     2221 gttacctttg atgtaagaaa ccatcctgaa cgcatggttg tcgaggatac caacaccgtc
     2281 agttttgtaa ttacaaaacc aaacggcaaa aaactcttta tcgatgaact gccagggaca
     2341 caccttacta gcgatgaact caccgagatc aagaaaaagg tagaggcagg tactgcaacc
     2401 gcagacgatc ttcgccgcat gaacgagatc aagaacgaac ttttccgcat catgcaggca
     2461 atcccggacg agaaattatt cgccgttcgc gaagaataac ttcgtcctaa cgatatttat
     2521 tttacgtcac aaccccaatg tgaaattgaa ttatgatgga ggatatcaca tccaccgtcg
     2581 agttccagat gcgtttgctg ctcttcgtgg cc
//
LOCUS       DALTZZ010000112         6350 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_17994,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000112 DALTZZ010000000
VERSION     DALTZZ010000112.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17994"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(267..1175)
                     /locus_tag="O0X12_02025"
     CDS             complement(267..1175)
                     /locus_tag="O0X12_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52971.1"
                     /translation="MQKKYILMILVICLLAAGISAAPAVARDVSPGSTIFVYEDGLRF
                     TDDLSDAAQLQYKSNNNVLNMISVQDPNNFNLLASSVGDYTGSWDVINSSGTKLGTVL
                     IYYPDLSIDIVLAKDGVSSVQEWGFLDTEYYRIKINSPHVGPSGIGAKVNVIFKGADG
                     SETTYVSGNSFADIAMNSVQVLTPQSFRPKDLGKTTTIYAEWSSPTSFHNYAATSNSI
                     TMEYNDVTDPGVSITTNPTSTPTTATPSVTVTTIPPTTVATTPIPVETTSDPTTEIPT
                     PSPTESSLPVYLVTLSLLGAFVLFRR"
     gene            1368..1937
                     /gene="trmY"
                     /locus_tag="O0X12_02030"
     CDS             1368..1937
                     /gene="trmY"
                     /locus_tag="O0X12_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HJK52972.1"
                     /translation="MLRFAILGHKATTSPDFSLNDMPGGAGRMDVLCRCINSSFFLSH
                     DLRRNTECYLILEGGEPHKTLRFSGETIRSLNPDERSAGALIKKGLSTPAEPEFRKAA
                     DGIAIRSAGLAELLQEFSFAVLDENGKDIRETETLPENFILSDHMNFTEEEEELLKDL
                     PRYSVGPRVLHADHTITVILNEFDRRERS"
     gene            1934..3175
                     /locus_tag="O0X12_02035"
     CDS             1934..3175
                     /locus_tag="O0X12_02035"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HJK52973.1"
                     /translation="MTDILEKVHQILEYGDICDHCLGRLFAKRSFGLTNEQRGHALRV
                     AHALAYNIPFVPYEKGTCWICNDLFDTVPAWAEKVATAMQDIEYSTFVIGTRVPPMMS
                     ESEEMIWSDLSLEQPEPIKSEMNREVGKAVSALTGKRGDPKNPEITAVLNIADDCVEL
                     QIASIYFYGRYLKYERGIPQTHWDCRACRGKGCEKCNFTGKQYPTSVEELIAEVPKQM
                     FAAENGVLHGSGREDIDALMIGTGRPFVMEMQNPKIRNINLQELESAINTSAAPRVQV
                     KLERWSDKKTVEMLKSHKGHKTYRILVDIDDSISLETVQNAVSKLKGALIHQRTPERV
                     AHRRADLIRERRVVDIECLGIEDSLFRIEVVGEGGLYIKELVSGDGGRTTPSLAEILA
                     VPAKVVALDVVQVDGINNGDE"
     gene            3178..3471
                     /locus_tag="O0X12_02040"
     CDS             3178..3471
                     /locus_tag="O0X12_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HJK52974.1"
                     /translation="MAKHNGIKKRTRYKLQKGLRERGMPNVTSVIQHFEEGQKVHIVI
                     EPSVQKGMPHPRFHGKTGTVLGQRGRAWLLEIKDGNATKVVISRPQHLKAQKY"
     gene            3514..3885
                     /locus_tag="O0X12_02045"
     CDS             3514..3885
                     /locus_tag="O0X12_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4 family protein"
                     /protein_id="HJK52975.1"
                     /translation="MKVKKVISEEMMSLPELREVLIAIRDQRAGGDSETETSARTMSY
                     ELRKSIDHADSLGKCDPATAKALVDELNGLEKIRPEIAYRIVNIMPESRDELRAIYAK
                     ERYTLLPEDLDQILDILRQHA"
     gene            3900..4478
                     /locus_tag="O0X12_02050"
     CDS             3900..4478
                     /locus_tag="O0X12_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HJK52976.1"
                     /translation="MPPKTERADKKEVEAIVLDFLQWGYADDKRPLNQREPVILAVGT
                     DQFKLLELIPKRNYAINLHDKVYIGDGERKVVERVKRRISYDELTNTAKGELEPVISM
                     IIAETEPRFVKFYNEAVPISLKLHMLNLLPGFGKKTLTDTLTERQKKPFESFEDIRSR
                     VKTLAKPEKFILERIMLELENPDEKYHLFTSK"
     gene            4475..5254
                     /gene="rsmA"
                     /locus_tag="O0X12_02055"
     CDS             4475..5254
                     /gene="rsmA"
                     /locus_tag="O0X12_02055"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HJK52977.1"
                     /translation="MKAPKDQHFLVDADAVALIADTVPVSGRKVLEIGPGGGVLTDAL
                     LQRGAVVRAVELDGTLIPNLNRRFSNQIASGQLEIIRGDASKVPLPEFDLVVANLPYS
                     ISSKITFRLLEIGFESAVLMYQLEFGKRMIAPPGNSEYGRLSVMAQTYAEVEMVLELP
                     PEAFSPPPEVWSIVVKITPHEPPTPICDRTVHATLVRELFSHRRKTIRNGLRGMRSIY
                     GEQPVTNLIATLPDELLEMRPEMLSITDFIDLANRLSALIP"
     gene            5251..5826
                     /locus_tag="O0X12_02060"
     CDS             5251..5826
                     /locus_tag="O0X12_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylase"
                     /protein_id="HJK52978.1"
                     /translation="MIPDTSQIYFPAEDTHLLIRAALAEVKESDRVLEVGTGSGAVAK
                     ALMTIAKKTAATEINPHAAAYAASEGVAVVRGNLLDPIRGEFDLILFNAPYLPTTPEE
                     RMNDWLEFALDGGETGRKVIEWFLPAAIDHLSLFGRILLLVSSSTGVPELLSLCKQYR
                     MIGIVADTERMEDGEMLYVLRISRDLCCIER"
     gene            5823..6110
                     /locus_tag="O0X12_02065"
     CDS             5823..6110
                     /locus_tag="O0X12_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HJK52979.1"
                     /translation="MTATLTEEISYQGHHSVGMTIQKIEFGVSGMHCGGCSGHLTTML
                     SELPGVDDVKVDHVAGTASLSVDYDQTTFDDITDCVLDAGFDVVPDSLKTL"
     gene            6165..>6350
                     /locus_tag="O0X12_02070"
     CDS             6165..>6350
                     /locus_tag="O0X12_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52980.1"
                     /translation="MVIMARTLSEHDIALLLKLAPECDTLICKGSENAYRSILPPVAN
                     HYAKDADDFAMRLNRLTD"
BASE COUNT         1793 a         1453 c         1636 g         1468 t
ORIGIN      
        1 acattgaaat gtatctttgt gtcgggatat aagtattctc tttaatagga gagaagggag
       61 atatctgcgt tgtttgtgac ttcaaggcag aaaatgcgaa attggtaaat actttctgga
      121 atttttcggg aaagttgcgg ccaagtgtga cgagacatac aggcaggtaa tactttgtgc
      181 gttcgataaa agggacagtg ataacacacc atgcgcccca ataaatcacg gtttctttga
      241 ccgcgcacgg gaaaaaggag gtttttttat ctccggaaca gaacaaatgc tccaaggaga
      301 gacaaagtaa ccaggtaaac agggagagat gactctgttg gagaaggtgt agggatctct
      361 gtagtcggat ctgatgttgt ttctacgggt attggagtgg tcgctaccgt agttggggga
      421 atggtcgtta cggtaacaga aggagttgct gtagttggag ttgatgtcgg gttagtggtg
      481 atgctcacac caggatcagt tacatcatta tactccatgg tgatggagtt ggatgttgcc
      541 gcatagttgt ggaaggaagt cggagaactc cactcagcat agatcgtggt ggtctttccc
      601 agatcctttg gacggaatga ttgaggtgtc aggacctgaa cactgttcat agcgatgtca
      661 gcaaaactat tgccggagac atatgtggtt tcagaaccat ctgccccttt aaagataaca
      721 ttcaccttag ctccaatgcc gctcggacct acatgcggcg aattaatctt gatccggtaa
      781 tactcagtat caaggaagcc ccactcctgc actgaagaaa caccgtcttt tgcaagaacg
      841 atgtcaatgg agagatcagg ataatagatc agaactgtac caagctttgt ccccgaactg
      901 ttaatgacat cccatgaacc ggtgtaatca ccaacagaag atgcgagcag gttgaaatta
      961 ttaggatcct gaacagaaat catgttgagg acattattgt ttgacttgta ctgcagttgt
     1021 gcagcatcag aaagatcatc ggtgaacctt agcccatcct cgtaaacaaa gatagtactc
     1081 ccgggggaga catcacgggc aacagccgga gctgcagaga tccctgcagc aagaaggcag
     1141 attacgagga tcataagaat gtattttttc tgcataaaat actcctcaaa cgtataaggt
     1201 gtatgtgggc tctcacgcta tagattattt gtggagaaaa agagcaacag aaagtacctc
     1261 tctacagcaa tcacttcact aactctgcaa atacaccaaa aaagaattcg gaacaaaccc
     1321 accgggcgaa cgtcttatgc cgattaacag ccaatgtata ttctcacatg ctccggtttg
     1381 caatcctcgg ccacaaggct acaacatccc cggacttctc cttaaacgac atgccgggag
     1441 gtgccggaag aatggacgtt ctctgccgtt gtatcaacag ttcattcttc ctcagtcacg
     1501 atctccgccg caatacagag tgttatctca tcctcgaagg aggcgagccg cacaagacac
     1561 tccggttctc cggagagaca atccgctcac tgaaccctga cgaacgaagt gccggtgcac
     1621 tgatcaagaa aggattgagt actcctgcag aaccagagtt caggaaagcc gcggacggaa
     1681 ttgcaattcg atctgcaggc cttgcagaac tgttacagga attttccttt gcagttcttg
     1741 atgagaacgg taaggatatt cgggaaacag agacactgcc ggaaaatttc atcttaagcg
     1801 atcacatgaa cttcactgaa gaggaggaag aactactgaa agatcttccg cggtactcgg
     1861 tcggcccccg ggtactgcat gctgatcaca caattacagt gatcttaaat gaatttgatc
     1921 ggagggaaag atcatgacgg atattctgga gaaggtacat cagattctgg agtacggtga
     1981 tatctgtgat cactgtctgg gaagactgtt tgcaaaacga tcgtttggcc taacgaacga
     2041 acagcgcgga catgcactgc gggttgctca cgctcttgca tataatatcc cgttcgttcc
     2101 ctacgagaaa ggtacctgct ggatctgtaa cgatctcttc gacactgtac ctgcatgggc
     2161 agaaaaagtg gcaaccgcca tgcaggacat tgagtacagt acctttgtga tcggtacacg
     2221 cgtcccgcca atgatgagcg aatcagaaga gatgatctgg tcggatcttt ctctggaaca
     2281 gcccgaaccg atcaaatcgg aaatgaaccg tgaggtgggt aaagcagtat ccgctctaac
     2341 cggaaaacga ggagacccaa agaaccctga gataacagct gtactgaaca tcgctgatga
     2401 ctgtgtggag ctgcagattg catccatcta cttctacggc agatacctca agtatgaaag
     2461 aggaatccca cagacgcatt gggactgccg cgcatgccgg ggaaagggat gtgaaaagtg
     2521 taatttcacc ggcaaacagt atccaacttc cgttgaagaa ctgattgcag aggttccaaa
     2581 acagatgttt gctgcggaaa acggagtatt acacggatcc ggcagagaag acattgatgc
     2641 actgatgata ggtaccggca gaccgtttgt gatggagatg cagaatccaa aaatccgaaa
     2701 tataaatctc caggaactgg aatcagcgat taatacatcc gccgcacccc gtgtccaggt
     2761 aaagctggaa cggtggtccg ataaaaagac ggtggaaatg cttaaatcac acaaagggca
     2821 taaaacatac aggattctgg tcgatataga cgacagtatc tctcttgaaa cggttcaaaa
     2881 tgcggtatca aagctaaaag gagctttgat tcaccagcgc acgcctgaac gcgtagcaca
     2941 tcgcagagcg gatcttatca gagagagaag ggtcgttgat atcgagtgtc tcggaataga
     3001 ggattccctc ttccgcatcg aggttgtagg agaaggagga ctctatatca aagaactcgt
     3061 atcaggagac gggggaagaa caactccgag tctcgctgaa atcctcgccg tccccgccaa
     3121 agttgtcgca ttagacgtgg tgcaggtcga cggaattaat aatggagatg agtaaaaatg
     3181 gcaaaacata acggtattaa gaaacggaca cggtataagc ttcagaaggg tcttcgtgag
     3241 cggggaatgc cgaacgtaac ttcggttatc cagcacttcg aagagggtca gaaggttcac
     3301 atcgtcattg agccgagcgt gcagaaaggc atgccgcacc cgcgtttcca cggcaagacc
     3361 ggtacggtcc ttggccagcg cggccgtgcc tggcttcttg agatcaaaga cggcaatgca
     3421 accaaagtgg tcatctcccg tccacagcac ctcaaggcgc agaaatatta acccccaaat
     3481 actaatttta catttatttc aggagtcaca ggcatgaaag tgaaaaaagt tatcagcgaa
     3541 gaaatgatgt cgctgccgga acttcgtgag gtactgatcg caattcgtga tcagcgtgct
     3601 ggcggagaca gcgagacgga aacgtctgca cggacgatgt cgtacgagct gcgcaagagt
     3661 atcgatcatg ccgacagtct cggaaagtgt gatcccgcaa ccgcaaaagc tcttgttgat
     3721 gagttaaacg gtcttgaaaa gataagacca gagattgcct accggattgt caatatcatg
     3781 ccggagagcc gtgacgaact tcgcgcaatc tacgcaaagg aacggtacac acttctcccg
     3841 gaggatctgg atcagatcct tgacattctc cgtcagcatg cgtgagtgag gtagaaataa
     3901 tgcctccaaa aaccgagaga gccgacaaaa aggaagtcga agctatcgtg ttagacttcc
     3961 ttcagtgggg atatgctgac gacaaacgtc cgttaaatca gcgtgagcca gtcatccttg
     4021 cagtcggtac cgaccagttt aaactgctgg agctgattcc gaaacggaac tatgccatca
     4081 atctgcacga caaagtgtac atcggagatg gcgagcgaaa ggttgtcgaa cgggtaaagc
     4141 gccgtatctc ttacgatgaa cttaccaata ccgcaaaagg tgaacttgag ccggtgatct
     4201 caatgatcat cgcagagacg gaaccacggt tcgtgaagtt ctataatgag gcagtgccca
     4261 tcagtctgaa actgcacatg ttaaatcttc ttcctggttt tggaaagaaa acactgactg
     4321 atacacttac cgagcggcag aaaaaaccgt tcgaaagctt tgaggatatc cgctcccggg
     4381 taaaaacgct tgcgaagccg gagaagttta ttcttgaacg gattatgctg gagctggaaa
     4441 atccggacga gaagtaccat ctcttcacgt caaaatgaag gctcccaaag atcaacattt
     4501 tttggtagat gcagatgcgg ttgcgctgat tgcagatact gttccggtat ccggcagaaa
     4561 agtccttgag atcggacccg gtggcggcgt actgacagat gctctgttac agcggggtgc
     4621 tgttgtcagg gcggttgagc tggacggaac actgatacca aatcttaacc gtcgtttttc
     4681 caatcagatt gcatcaggtc agctggagat aattcgcggc gatgcatcaa aagttcctct
     4741 gccggagttt gacctggttg ttgcaaacct gccgtactca atctcctcaa aaatcacctt
     4801 ccgattgctg gagatcgggt ttgagtcagc agtgctgatg tatcagctgg agttcgggaa
     4861 acgaatgatt gcacctcccg gtaacagtga gtacggaaga ctctcggtga tggcacagac
     4921 atatgcggag gtggagatgg ttctggaact cccgcctgag gcattctccc cgccaccgga
     4981 ggtgtggtca attgtggtga agatcacacc gcatgaacca ccaacaccaa tttgtgatcg
     5041 aacggttcac gcaacactcg tcagagaact gttctcccac cggagaaaga caatcagaaa
     5101 cggactgcgc ggcatgagga gcatctacgg agaacaaccg gtgacaaatc ttattgcaac
     5161 tcttccagac gagctgctgg agatgcgccc cgagatgtta tcgatcacag atttcattga
     5221 tcttgcaaac agactctctg cactgattcc atgataccgg atacctcgca gatctatttc
     5281 ccggcagagg atacacacct gctgatccgc gctgcccttg cagaagtcaa agagtctgac
     5341 cgggttttgg aggtaggaac cggttccggt gcggtcgcca aggcactaat gaccattgcc
     5401 aagaaaacag cagcaacgga gatcaatccg catgccgcag catatgccgc aagcgaaggt
     5461 gtagctgttg tccggggaaa cctgctggac cccatccgcg gcgagtttga tctgattctg
     5521 tttaatgcac catacctccc gacaacgccg gaagagagaa tgaatgactg gctggagttc
     5581 gcattggacg gaggtgagac gggacgcaag gtaattgaat ggtttctgcc tgcggcaatc
     5641 gatcatctct cactgtttgg cagaatcctt ctcctggttt cgtcatcaac aggagttccg
     5701 gaacttcttt cgctgtgcaa acagtaccgg atgataggaa ttgtggcaga tacagagcgg
     5761 atggaggacg gcgagatgtt gtatgttctg cggatctcac gggacctctg ctgcatagag
     5821 agatgacggc aaccctaaca gaagagataa gttatcaggg acaccattct gttggtatga
     5881 caatccagaa gattgagttt ggtgtctccg gtatgcactg cggagggtgt tccggccatc
     5941 tgacaacgat gctttcggaa ctgccgggtg ttgacgacgt aaaggtcgat cacgttgcag
     6001 gtactgcatc gctttccgtt gactacgatc agacaacatt tgatgacatc acagactgcg
     6061 ttctggatgc agggtttgat gttgtacctg actcactgaa aactctctaa ccatttttct
     6121 tttcagtttt gtatccgaca agtaattacg cgagtatcat aataatggta attatggcca
     6181 gaacactctc cgagcatgac atcgcactac tcctaaaact ggcacccgag tgtgataccc
     6241 tgatctgtaa aggatcagag aacgcctacc gttcaattct cccaccggtt gcaaaccatt
     6301 atgcaaagga tgctgacgat ttcgcaatgc ggctgaatcg tttgacagac
//
LOCUS       DALTZZ010000113         1835 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_18226,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000113 DALTZZ010000000
VERSION     DALTZZ010000113.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18226"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(133..399)
                     /locus_tag="O0X12_02075"
     CDS             complement(133..399)
                     /locus_tag="O0X12_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HJK52981.1"
                     /translation="MIELFPSYYSILHVLFLAAMILSAVAVFYLKDLIAAAIGFAAFS
                     FLLALEFFILQAPDVAIAEAAIGAGISTAILIIAIRGTTREESE"
     gene            complement(396..776)
                     /gene="mnhG"
                     /locus_tag="O0X12_02080"
     CDS             complement(396..776)
                     /gene="mnhG"
                     /locus_tag="O0X12_02080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HJK52982.1"
                     /translation="MIETAIIILVVLSLFFSILGVIGLFRFPDFYTRIHAAGLVGSFG
                     VLFAGFAVLLYAYTLWAAGEEAWFNYGAHVLLALVVVVVTATTSTHAIARSAYRSGNT
                     PKVHVIDALEEDEPKMMAQEAARK"
     gene            complement(773..1033)
                     /locus_tag="O0X12_02085"
     CDS             complement(773..1033)
                     /locus_tag="O0X12_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HJK52983.1"
                     /translation="MIDIFMIAAIIFVLLIFACGVRMWLGPTTADRMLALDVINALVV
                     IIMIILSIRFEQPGFIDVAIVYALLSFVGTLYVAKLIRGDLE"
     gene            complement(1030..1569)
                     /locus_tag="O0X12_02090"
     CDS             complement(1030..1569)
                     /locus_tag="O0X12_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HJK52984.1"
                     /translation="MKSIVPFISATVAAFIIYLVLSVGSAGETNSILLWSVPELIIGL
                     ILSIITGLLCRKLWQGKRYTMANPLRWLLLAVYIIPFVIELIIANLTVSWKIITGKNI
                     RPGIVKLTPGLKTDAGALLLSASITFQPGTATVDINEKTREMYIHCIDVGEAPVKCME
                     PGKIFAKLDLVKWIRRITE"
BASE COUNT          455 a          416 c          559 g          405 t
ORIGIN      
        1 atatagtatt gatcggtgct tgactccagc ggatggccga aggtgaggcc gaacgccggc
       61 agaagcagga taagggtgac tgcaatgatc aaaaagatcg caagctttgg gggaacggaa
      121 caacgtgcca tcttactcac tctcctcgcg ggttgttccg cggatggcga tgatgagaat
      181 ggcggtggag attccggctc cgattgcagc ctccgctatt gcaacatcag gggcctgcag
      241 gatgaagaac tcaagcgcca gaaggaagct gaacgcggca aatccgattg ctgcggcgat
      301 gaggtctttt aagtagaaga cggcgaccgc cgagaggatc atggcagcaa ggaagagaac
      361 gtgcagtatc gagtagtagg atgggaaaag ctcgatcatt tgcgtgcggc ctcctgtgcc
      421 atcatctttg gttcgtcctc ttcgagggca tcgattacat gtactttcgg ggtgtttccg
      481 ctccggtatg cactgcgggc gatggcatgg gttgaggtgg ttgcggttac gaccacgacg
      541 acgagtgcca gaagaacatg ggctccgtag ttgaaccagg cttcctctcc tgcggcccag
      601 agcgtgtagg catacagcag tacggcgaat ccggcaaaca gtacaccgaa acttccgacg
      661 agtcctgcgg catggattct ggtgtagaag tccgggaacc ggaagagacc aatcacgccg
      721 aggatactga agaacagcga cagcacaaca aggatgatga ttgctgtttc aatcattcga
      781 gatcacctct gatgagtttt gcgacgtaga gtgtgccgac aaaggagagc agggcataca
      841 cgatggcaac atcgatgaag ccgggctgct caaagcggat ggaaaggatg atcatgatga
      901 tgacaacaag agcgttgatg acatcaagtg cgagcatccg atcggcggtg gtcgggccaa
      961 gccacatccg gactccgcat gcaaagatga ggaggacgaa gatgatggcg gctatcatga
     1021 agatgtcaat cattcggtaa tcctccggat ccatttgacg agatcgagct tggcaaagat
     1081 tttgccgggc tccatacact tgaccggtgc ctcgccgaca tcgatacagt ggatgtacat
     1141 ctcgcgggtt ttttcgttga tatcaacggt tgcggttccg ggctggaagg tgatggaggc
     1201 cgagagcagg agtgctccgg catccgtttt caggccgggt gtgagtttga cgatacccgg
     1261 acggatgttt ttgccggtga tgatcttcca cgaaactgtc aggtttgcaa taatcagttc
     1321 gattacaaac ggaatgatat agacggcaag cagaagccag cggagagggt tggccatggt
     1381 gtagcgtttt ccctgccaga gcttgcggca tagcagcccg gtaataatcg aaaggattag
     1441 tccaatgatg agttcgggta ctgaccagag aagaatcgag ttggtttctc cggcacttcc
     1501 aacggataat accagataaa tgataaatgc agcaacggtt gctgagataa acgggacgat
     1561 gcttttcatg ggcagagcag tccccctgtt gttgtgttca tctgtttgtt catacacacc
     1621 tccctacggc agtagggtat tgcacgccac catcggtcgt gccaggttct tcccgtcgga
     1681 agaacggttg cgggtcacct ccggacctgc atctattata atggtgtgta tgagcaatta
     1741 agatgtgctc cctgacgagg aggatgtggg aatttccggg tgggttattc ccaggatgag
     1801 gagagtgatt ccggcaagga tgcttacgac aaagg
//
LOCUS       DALTZZ010000114         4299 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_18284,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000114 DALTZZ010000000
VERSION     DALTZZ010000114.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18284"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="O0X12_02095"
     CDS             <1..712
                     /locus_tag="O0X12_02095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HJK52985.1"
                     /translation="IPGAVPALQELKRRGIPFRFVSNNTHRSRETIQARLARFGVDIP
                     VEWIFTPLTAAIAYLRQAGATSCWLLGSPDAAKELTNAGINPADPNASHVLIGDLSDV
                     LGYEMFVAGFRVLMRNHAELLALEHDRYFKGSDGLLLSAGAFVAALEFAADTSAKLLG
                     KPSAAFFQAALSSLGLPPEEVVMIGDDPRSDIGGAATVSVRGVLVLTGKFDGVLPDGV
                     VAPWKILPGLADALDLTQ"
     gene            complement(702..857)
                     /locus_tag="O0X12_02100"
     CDS             complement(702..857)
                     /locus_tag="O0X12_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52986.1"
                     /translation="MTSIALTSMAERAAQYTVIQRKNQLSKLCWYAAGTPGNMIPSLK
                     DPTYLIA"
     gene            986..1714
                     /locus_tag="O0X12_02105"
     CDS             986..1714
                     /locus_tag="O0X12_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK52987.1"
                     /translation="MLEISNLHVEVGGREVLHGVNLKINDGETHVLLGPNGSGKTTLL
                     RTIMGFGSSQVTSGKILFNGVDVTEKPVHERAQLGMGIMFQRPPTISGLKLGKFLTAT
                     TKIDEERIPSLAERMHMTKFLDRDVNAGFSGGEIKRSEILQLMVQNPSFVMLDEPESG
                     VDVENMALLGNAAASLLEKDQHMINRTKSGLIITHTGYILDYIEADVGHVLIDGVMRC
                     SGNPREILRSVKTSGYKECIACQK"
     gene            1702..2811
                     /locus_tag="O0X12_02110"
     CDS             1702..2811
                     /locus_tag="O0X12_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HJK52988.1"
                     /translation="MSEMKGFDSISTEDKERLALTGIQTDSMEGRAGSFLLVNDHVLH
                     AGSNAEGVEVMMIDKALEKYAWLADYCWNVVPKDKDQYTQYVADHPQRGYVIIARKGA
                     KTTFPLQSCMFLAGDAIQAVHNIVIAEEGAEVHLIAGCASSMKTKEGAHYGINEIYVG
                     KNAKVTSTMIHTWGEEIEVFPRTSSVVEEGGTFISNYVCMKPTKMVQMYPTAHLRGKG
                     AVARFSSVIVAGPGSHIDAGSRAVLEAPETSAELITRAITNGGTIISRGAIVGEAPQT
                     KGHIECRGLILKDGIMHAIPEIDGRVVDVELSHEAAVGKIARDEIEYLMARGLSEEEA
                     TATIIRGFLDVRIEGLPDALQKQIDDAIDSADHGF"
     gene            2811..3371
                     /locus_tag="O0X12_02115"
     CDS             2811..3371
                     /locus_tag="O0X12_02115"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HJK52989.1"
                     /translation="MRIYDVSRTLAPGMYVYPGDPEFSMTPLRSGESYISALSFGTHT
                     GTHIDAPAHYFSGGAGIDKQPLSTFITTVELLPFGEQISGTAPAVLLRSGYKEGDSAY
                     PQLSEKEACSLVESGIEIVGCDTPSIGNDAVHRILLAADVVVIELLDFTDVPDGQYNM
                     VALPLKIAGADAAPARVVLIEEEERV"
     gene            3368..3958
                     /locus_tag="O0X12_02120"
     CDS             3368..3958
                     /locus_tag="O0X12_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52990.1"
                     /translation="MILHDAIVRLREVSTGAGCEDGDLRYAPLPAHHVCRYCRGRCLG
                     VEYGGRVAEISSPEPFSARMLLEHLFDAPLKSEKTRAAAAGALTTAAGFLMLTRKLAP
                     CPTVNFDDCLEELVARCAGQQVYVIGDDIPGVRQALFVEEADLVIATGDALLTDDCLE
                     EIEEARSLGKEILLLGPEWAGTAALLGMKHWCPYGT"
     gene            complement(3962..4204)
                     /locus_tag="O0X12_02125"
     CDS             complement(3962..4204)
                     /locus_tag="O0X12_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52991.1"
                     /translation="MYVITPTLFFIGAGVFIVGIFFTNPLYLLLLTLALVFVSLVWKG
                     NLLTSFVTNQVYLLAGLYSPRKDAVIWESTSKKVGE"
BASE COUNT         1038 a         1067 c         1200 g          994 t
ORIGIN      
        1 gattccaggc gcggttcccg ctctgcaaga gttgaagcgg cgcggcattc ctttccggtt
       61 cgtttccaac aacacgcaca gaagcaggga aaccattcag gcacgtcttg cacgattcgg
      121 cgtggacatc ccggttgaat ggatctttac cccccttact gccgcaatcg cctatctccg
      181 gcaagcagga gctacctcat gctggctgct tggaagtccc gacgccgcaa aggaacttac
      241 aaacgctgga attaatcccg cagacccaaa cgcgtcacac gttctgatcg gcgacctgag
      301 cgatgtgctg gggtatgaaa tgtttgttgc aggcttccgt gtattgatgc gtaaccatgc
      361 cgaactgctt gcccttgaac acgatcgcta cttcaaagga agtgacgggc ttctgctctc
      421 ggcaggtgca ttcgtagcag ctctcgagtt tgccgcagac acatccgcca aactgctggg
      481 aaaaccatcc gcagcatttt tccaggcggc actctcttcc cttggtcttc ctccggaaga
      541 agtggtcatg ataggtgatg atccgcgttc ggacatcggc ggtgcagcca ctgtatccgt
      601 gcggggtgtt ctcgttctca ccggcaagtt tgacggagtg cttcccgacg gtgtagtagc
      661 tccctggaaa atactcccgg gacttgcgga tgctcttgat cttacgcaat aaggtaggtc
      721 ggatccttca gagacggaat catgttgccg ggagttcccg cagcatacca gcagagcttg
      781 gataactggt tcttgcgctg aattacagta tactgcgcag cacgttctgc cattgaggtt
      841 aatgcgatgc ttgtcatgat caatcatcag aatggaggga tataaacccc ggaccgcgcg
      901 tactgaaagt gccctcccat ctggaaaacg taatcaaaca cgtatcttta caaccctcca
      961 caacaaatta catagcagat tttccatgct tgagatcagc aatcttcatg tggaagttgg
     1021 cgggcgtgaa gtccttcacg gcgttaatct caaaattaac gatggtgaga cccacgtact
     1081 tctcggaccg aacggctcgg gaaaaaccac ccttctgcgt accatcatgg gatttggcag
     1141 ttcccaggtc accagcggca aaatactttt taacggcgtt gatgtcaccg aaaaaccagt
     1201 gcacgaacgg gctcagcttg gtatgggtat tatgtttcag cgtcccccca ctatctccgg
     1261 cctgaaactt ggtaagtttc tgaccgcaac aaccaagata gacgaggagc gtatcccttc
     1321 gctggcggaa cgcatgcata tgacaaagtt cctcgatcgt gatgtcaatg ccggattctc
     1381 cggcggcgag atcaaacgca gtgaaatcct ccagctcatg gtgcagaacc cctctttcgt
     1441 catgctggac gagccggaaa gcggtgttga tgtcgagaac atggcactgc tcggaaatgc
     1501 tgcggcaagt ctgctggaaa aagatcagca catgattaac cgcaccaagt ccggcctcat
     1561 tatcacgcac accggctaca tcctcgacta catagaagcc gatgtcggcc acgtcctgat
     1621 cgacggcgtg atgcggtgca gcggaaaccc gcgtgaaatt ctccggagtg taaagacatc
     1681 cggttacaag gagtgtatcg catgtcagaa atgaaaggat ttgactccat cagcaccgag
     1741 gataaagagc ggctggcact gacaggcatt cagacagatt ccatggaggg acgtgccggc
     1801 agtttccttc ttgttaatga ccatgttctc cacgcaggct caaatgccga gggcgttgag
     1861 gtcatgatga tcgacaaagc actggagaag tatgcatggc tggcagacta ctgctggaat
     1921 gttgtaccaa aagacaaaga tcagtacacg cagtacgttg ccgatcatcc ccagcgcggc
     1981 tatgtaatca tcgcccgcaa gggtgccaag accaccttcc ccctgcaaag ttgtatgttc
     2041 cttgccgggg acgccattca ggcagtgcat aatattgtaa ttgccgagga aggggccgag
     2101 gtccatctga ttgccggatg tgccagttct atgaagacga aagagggtgc acactacggt
     2161 atcaatgaga tctacgtcgg caagaacgca aaggtgacct caaccatgat ccacacatgg
     2221 ggtgaggaga tcgaggtgtt tccgcggaca tcttctgttg ttgaagaggg aggaacgttc
     2281 atctcaaact atgtctgcat gaagccaacc aagatggtgc agatgtatcc taccgcacat
     2341 ctccgcggga agggagcggt cgcccgtttc tccagcgtga ttgtggcagg gcccggatca
     2401 cacattgatg cgggttcgcg tgccgttctg gaagccccgg agacaagcgc tgagttgatc
     2461 acccgtgcga ttacaaacgg cggtacgatt atctcccgcg gagcaatcgt tggtgaagct
     2521 ccgcagacaa agggacacat tgagtgccgc ggtctgattc tgaaggacgg aatcatgcat
     2581 gcaatcccgg agattgacgg gcgtgtggtg gatgtggagc tctctcatga ggcagcggtc
     2641 ggaaagattg cccgcgatga gattgagtac cttatggcgc gcggtctttc tgaggaggag
     2701 gcaactgcaa cgatcatccg cggattcctt gatgtgcgca ttgagggtct gccggatgca
     2761 ctgcagaagc agatagatga cgcgattgat tccgcagatc acggattctg atgagaatat
     2821 atgacgtgag ccggacgctt gcgcccggga tgtatgtgta tccaggcgat ccggaattca
     2881 gcatgacacc tctgcgctca ggggagtctt acatttccgc gctgtcgttt gggacacata
     2941 cagggacaca tattgatgcc cccgcacact atttttctgg gggagctggg atagacaaac
     3001 agccactcag tacgttcatt acaactgtcg agctgcttcc ctttggtgaa cagatatcag
     3061 gaacagcacc tgcggtacta ctcagatcag gatacaagga gggagattct gcatacccgc
     3121 aactttcaga gaaagaggcg tgttccctcg tagagtcagg aatagagatt gtcgggtgtg
     3181 ataccccgtc gattggtaat gatgcggttc accgcattct cctggcagca gatgttgtag
     3241 tgattgagct gctggatttt acggatgtac cggacggtca gtacaacatg gttgcactgc
     3301 cgctgaaaat tgctggagct gatgcggctc ctgctcgggt tgttttaatt gaggaggaag
     3361 aaagagtatg attttgcatg atgcaattgt gcgcctgcgt gaggtttcaa ctggtgccgg
     3421 atgtgaggac ggggatttgc ggtatgcacc cctgccagca catcatgtct gccgctattg
     3481 tcgcggcaga tgtctgggtg ttgagtatgg cggacgggtt gcggagatca gctccccaga
     3541 gccgttttcg gcacggatgc ttcttgagca tctgttcgat gccccgctaa agtcagagaa
     3601 aacacgagcg gcggctgcag gcgcactgac tacggcggcg ggttttctga tgttgacccg
     3661 taagcttgcg ccgtgtccga cagtgaactt tgatgactgc ctggaagagt tggtcgcccg
     3721 ctgtgcagga cagcaggtct atgtgattgg tgatgatatt cccggagttc gccaggcact
     3781 atttgtcgag gaggcagatc ttgtgatcgc aaccggtgat gcgcttctga ctgatgattg
     3841 tctggaagag attgaggaag cacgctccct tggaaaagag attctgctgc tcggaccgga
     3901 atgggccgga acagcagcat tgctggggat gaagcactgg tgcccgtacg ggacgtgaaa
     3961 cttattctcc cacttttttg gaggtgctct cccaaattac agcatcctta cgtggagaat
     4021 agagacctgc aagcaggtac acctgattgg taacaaagga tgtcaagaga tttcctttcc
     4081 aaacgaggct gacaaagacc aatgcgagag tcagaagcag aaggtaaaga ggatttgtga
     4141 agaagatccc tacaatgaac acaccagccc cgataaagaa cagcgttggt gttattacat
     4201 acatccagat cctcaaggga taaaatattc tggaaaaggg tcgttttaac atcagcaaat
     4261 cacggtttgc gagggttgct tgaattaatc cctttgcac
//
LOCUS       DALTZZ010000115         1557 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_18347,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000115 DALTZZ010000000
VERSION     DALTZZ010000115.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18347"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="O0X12_02130"
     CDS             <1..334
                     /locus_tag="O0X12_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833710.1"
                     /note="Shwachman#Bodian#Diamond syndrome protein family;
                     SBDS; similar to eukaryotic proteins involved in RNA
                     metabolism or binding; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome assembly factor SBDS"
                     /protein_id="HJK52992.1"
                     /translation="RIEIALEEVRVNFDPFKSVDELVKETVKALRPILPIRFEERKIA
                     VRFPMDCAARAFGAVSGAAYVTMEKNEWQNDGSWICVVKIPAGMQEEFFSLANHVAKG
                     DAQIKILE"
     gene            385..669
                     /locus_tag="O0X12_02135"
     CDS             385..669
                     /locus_tag="O0X12_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37ae"
                     /protein_id="HJK52993.1"
                     /translation="MASKSKHVAKGRVTGSAGRFGPRYGRFNRKMVNESEKISRAKHL
                     CPMCDTVALKRVGTGIWECKKCGYKYAGGAYVPQTPALKVVLRSIETKGV"
     gene            674..805
                     /locus_tag="O0X12_02140"
     CDS             674..805
                     /locus_tag="O0X12_02140"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit P"
                     /protein_id="HJK52994.1"
                     /translation="MVSYKCARCKQTVEIDTNVRCPYCGHRILFKARGAGMKVLKAR"
     gene            802..1203
                     /locus_tag="O0X12_02145"
     CDS             802..1203
                     /locus_tag="O0X12_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK52995.1"
                     /translation="MTFVTSSRKPSPEVRRLAKEIAFALDLPYVQRGKAGLRTMDNED
                     PVIIFLSGAKRGGQIFDLTVSGKIVFSMLITNVLTSERTGPFHHGFVTRERELYDALS
                     PHLPMMFDAEAPGPIVFSGTQKMQYILQVMV"
     gene            1200..1451
                     /locus_tag="O0X12_02150"
     CDS             1200..1451
                     /locus_tag="O0X12_02150"
                     /inference="COORDINATES: protein motif:HMM:NF020899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HJK52996.1"
                     /translation="MTGEQSQPTHTAEFRFVCPYAEKLYAVLAPEAGSDPGEKSRVRI
                     SRDGDAVTLFVEAEDASALRASLNMWLRLVNVCKEVLEL"
BASE COUNT          393 a          362 c          449 g          353 t
ORIGIN      
        1 ccgtattgag attgcccttg aggaagtacg cgtgaacttt gatccgttta agtcagtgga
       61 cgagctggtc aaagagacgg taaaggcact gcgcccgatt cttccaatcc ggtttgagga
      121 gcgcaaaatt gcagtccggt tcccgatgga ctgtgcggca cgtgcgttcg gcgcggtttc
      181 gggtgccgca tatgtgacga tggagaagaa cgagtggcaa aatgacggat catggatttg
      241 tgtcgtcaaa atccccgcag gaatgcagga ggagtttttc agcctggcaa accatgtggc
      301 aaagggcgac gcccagatca agattttaga ataagattaa aacatctgag aaccaataat
      361 agtagatatt tctgaggaat tactatggca tccaagagca agcacgtagc aaagggacgc
      421 gtaaccggca gtgccggacg ttttggcccg agatatggcc gtttcaaccg taagatggtc
      481 aatgagagcg agaagatctc ccgcgcaaag cacctgtgtc cgatgtgtga caccgttgcc
      541 ctgaagcgtg ttggaaccgg aatctgggag tgcaaaaagt gcggatacaa gtacgccggt
      601 ggcgcatacg tcccgcagac tcccgcttta aaggtcgttc tccgttctat cgagaccaaa
      661 ggagtataac taaatggtca gttataagtg cgcccgctgt aaacaaacag ttgagattga
      721 cacgaatgtc cgctgcccct actgcgggca ccgtattctc tttaaggcgc gcggtgccgg
      781 aatgaaagtg ctgaaagccc gatgacgttc gtaacttcat cgcgcaaacc gtcaccggaa
      841 gtgcgcagac tcgcaaagga gattgctttt gcacttgatc tcccgtatgt tcagcgggga
      901 aaggcaggtc ttcgaacaat ggataacgaa gacccggtaa taatttttct gtccggcgca
      961 aaacgcggtg gtcagatatt tgatctgacc gtgtcaggga agatcgtttt ttccatgctg
     1021 attaccaatg ttctgacaag tgaacggaca ggcccattcc accacgggtt tgtgaccagg
     1081 gagcgcgagt tgtatgatgc attgtcaccg catcttccca tgatgtttga tgccgaggca
     1141 ccaggaccga tcgtgttttc cggaacccag aagatgcagt atattctgca ggttatggta
     1201 tgaccggtga gcaaagtcag ccgacgcaca ccgcagagtt ccggttcgtg tgtccttatg
     1261 cggagaaact gtatgcggtt cttgcgccgg aggcaggatc ggatccggga gagaagtccc
     1321 gtgtacggat ttcccgagat ggagatgcgg tgacactctt tgtggaggcg gaggacgcat
     1381 cggctctccg tgcatcactg aatatgtggc ttcggttagt gaacgtgtgc aaagaagtat
     1441 tggagttgta aaaatggcaa acccacaggc gggcattccc ccgcagattc agcagcagct
     1501 ggcaatgttt cagcagattc agcagcagct gcagcaggta tcttcccaga agatgca
//
LOCUS       DALTZZ010000116         3432 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_18354,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000116 DALTZZ010000000
VERSION     DALTZZ010000116.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18354"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="O0X12_02155"
     CDS             <1..601
                     /locus_tag="O0X12_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HJK52997.1"
                     /translation="LYPCIALSDVVISCTAAPHAIITGEPLAATMNERLWPLDPEPRK
                     LILIDIAQPRDTEDSCRNIPGVHLFTIDDLRSVSEKNMEHRKDEAAHAGEMIDEYLPE
                     FIRLYNRTAAGDLIAGLYTWAESIRVRERDKALGKLGSPDPHTLAVIDDLTRVLTKKL
                     LADATLSIRASAECTDITTARKLVDAITRGEQVCFLKHD"
     gene            580..1554
                     /gene="hemB"
                     /locus_tag="O0X12_02160"
     CDS             580..1554
                     /gene="hemB"
                     /locus_tag="O0X12_02160"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HJK52998.1"
                     /translation="MFPQTRLRRLRRQSLRPLFTETHVTINDLIMPLFFVEGAAEKVP
                     IASMPGQFRWPLSAAADVAKDLAADGIKAVILFGIPKTKDAVASSAFASDGVIQEATR
                     AIKAAVPEMVVITDLCACEYTDHGHCGIIHESCDGPDLDNDASLELMQKIAVSQAEAG
                     ADMVAPSCMLDGMVTAIRTALDEAGYEYIPIMSYSTKFASALYGPFREAAASGFSFGD
                     RSTYQMNPANGREAFRESLLDMEEGADILMVKPASFYLDVLAQVRELGLPTAAYQVSG
                     EYSMIKAAAQNGWIDEKRIVMESLTSIKRAGADLIITYYAQDVARWLA"
     gene            1551..2792
                     /locus_tag="O0X12_02165"
     CDS             1551..2792
                     /locus_tag="O0X12_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HJK52999.1"
                     /translation="MNQNEALFHKAQTLLPGGVSSPVRAIKPYPFYVKSAKGAVLTTV
                     DDTRLVDCCLGYGPLILGHANPVIKAAISSQLDNGWLYGTPTELEIDLAERICNDHPS
                     MDMLRFVSTGTEATMAALRLARGFTGKTDIVKIEGGFHGAHDAVLIAAGSGATTHGTP
                     DSAGVLPDFAAHTRQVPYNDPEALETLLSKNDNIAAFIIEPVLGNIGPVLPDDGYLKA
                     VREITAAHDVLLIFDEVITGYRLGIGGAQVKFDIKPDLTTLGKIAGGGLPIGVFGGRR
                     EIMEMISPAGPVYNAGTFNANPATMAAGIAMNTYLHSHEDIYAKLDEKTRTIEESLPA
                     GASGTFVRLGSMFKYFFRSSAPRNYTEAKQCDTGAFRVFWEKALAAGVFLPPSQFETN
                     FLSAAHGDAEMTKLVQVYGQC"
     gene            2789..>3432
                     /gene="hemC"
                     /locus_tag="O0X12_02170"
     CDS             2789..>3432
                     /gene="hemC"
                     /locus_tag="O0X12_02170"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HJK53000.1"
                     /translation="MNVRIGTRGSKLALAQTNNVLRLLTDAGISAESQIITTSGDAVL
                     DRGLHQIGGQGVFVRELDNAILRGEIDAAVHSMKDIPAERPEGLITAAILPRDPPYDF
                     FVFNCPVEKIFSVGTSSTRRRAQLLRYYHCMPQVRVEPLRGNVDTRLAKLNDGMYDAI
                     VLAEAGLVRLGYHVNGVRLPAEHFVPSPNQGTVAVVCRDTPELREAFASLNDAQT"
BASE COUNT          787 a          932 c          925 g          788 t
ORIGIN      
        1 gctctacccc tgtatcgcct tgtctgatgt tgttatctcc tgtactgcag ccccgcatgc
       61 catcatcacc ggtgaaccgc ttgctgcaac catgaatgaa cggctctggc cgcttgatcc
      121 cgaaccgaga aaactaatcc tcattgacat tgcacagccg cgtgataccg aggactcctg
      181 cagaaatatt ccaggtgtcc accttttcac catcgacgat ttgcgttctg tttccgagaa
      241 gaacatggag caccgcaaag atgaggcggc tcatgcaggt gaaatgatag atgagtacct
      301 gcctgagttc atccggctct acaaccgcac agccgcaggt gatctgatcg caggacttta
      361 tacttgggca gagtcaatcc gtgtacgaga acgggataaa gccctcggaa aactgggatc
      421 tcccgacccc cacacactcg ccgtgatcga cgatctgacc cgtgtcctga ccaaaaaact
      481 ccttgccgac gcaacccttt caattcgggc gagcgccgaa tgtaccgata tcacaacagc
      541 ccggaagctg gttgatgcaa ttacccgtgg tgaacaagta tgtttcctca aacacgatta
      601 agacggctga gaagacagag tctgcgtcct ctgtttactg agacccatgt aacgatcaat
      661 gatctcatta tgccgctgtt ctttgtagaa ggagcagcgg aaaaagttcc tatcgcctcg
      721 atgcccggtc agttccgctg gccgctctct gctgccgctg acgttgcaaa agatcttgca
      781 gccgacggta tcaaagcagt gattctcttt ggtatcccca agaccaaaga tgccgttgcc
      841 agctcggcct ttgcttctga cggtgtcatt caggaggcaa cacgtgccat taaggcggcg
      901 gttccggaga tggtggtgat tactgatctt tgtgcatgcg agtacaccga tcacggacac
      961 tgcggcatca tccatgagtc ctgtgacggt ccggatctgg acaatgatgc atcgcttgaa
     1021 ctgatgcaga agatcgccgt cagtcaggca gaggcaggtg ccgacatggt tgcaccgtcc
     1081 tgcatgctgg acggcatggt gacggcaatt cgtacggcat tggatgaagc aggatatgag
     1141 tatattccaa tcatgtccta ctcgacaaag tttgcatccg ccctgtatgg tccgttccgt
     1201 gaagcagcag cctccggctt ctcgttcggc gaccggtcca cgtaccagat gaacccggca
     1261 aacggccgtg aggcgttccg cgagtcattg cttgacatgg aagagggtgc agatattctg
     1321 atggtcaaac ctgcaagttt ctatctggac gttcttgccc aggtcaggga actcggtctt
     1381 cccaccgccg cctaccaggt gtcaggagaa tactccatga tcaaagccgc agcccaaaac
     1441 ggatggattg atgagaaacg gattgtgatg gagtccctga caagtatcaa acgtgcgggt
     1501 gcggatctga tcatcaccta ctatgcacag gatgttgcga ggtggcttgc gtgaatcaaa
     1561 acgaggcttt gttccacaag gcacagactc tgctgccagg cggtgtttcc agtccggtcc
     1621 gtgcaatcaa accgtatccg ttctatgtga aatccgcaaa gggtgccgtg ctgacaaccg
     1681 tcgatgacac ccgtcttgtt gactgctgcc ttggatacgg tccgctgatc ctcggacatg
     1741 caaaccccgt gattaaagct gccatctctt cacagcttga caacggctgg ctgtatggca
     1801 cgcctaccga gctggagatt gatcttgcgg agcggatctg taatgatcat ccgtcaatgg
     1861 atatgctccg ttttgtctca accggtaccg aggctacgat ggctgccctg cgtctcgccc
     1921 gtggatttac cggtaagacg gacatcgtca agatcgaggg cgggttccac ggtgcgcacg
     1981 atgcagtgct gattgccgca ggttccggtg caaccacaca cggtacccct gactcggcag
     2041 gagttcttcc cgactttgca gcacatacgc gacaggttcc ctacaatgat cccgaagcac
     2101 ttgagaccct gttgtcgaaa aacgacaaca tcgcagcgtt catcattgaa ccggtgctgg
     2161 gaaacatcgg tcctgttctt cccgatgacg gatacctgaa agcagttcgt gaaattactg
     2221 cggcgcatga tgtcctcctc atcttcgacg aggtgatcac cggctaccgt cttggtatcg
     2281 gcggtgcaca ggtgaagttt gacatcaaac cggatctgac cactcttgga aaaattgccg
     2341 gaggcggtct gccgattgga gtatttggtg gtaggcgcga gattatggag atgatctctc
     2401 ccgcaggccc ggtgtacaat gcaggtacgt tcaatgcaaa cccggcaaca atggcggctg
     2461 gtattgcgat gaacacatat ctccatagcc atgaggacat ctacgcaaaa ctggatgaaa
     2521 aaacacggac aatcgaggag tctcttccag ccggggcatc cggaacgttt gttcggctcg
     2581 gttcgatgtt caagtacttc ttccgttcat ctgcaccaag gaactacacc gaggcaaagc
     2641 agtgtgatac cggtgcgttc cgtgttttct gggaaaaagc acttgcagcc ggtgtgttct
     2701 tacctccatc acagtttgag acgaacttcc tctctgctgc ccacggtgat gcggagatga
     2761 caaaacttgt gcaggtgtac ggacaatgct gaacgtacgg atcggcaccc gcggcagcaa
     2821 actggcactt gcacagacaa ataatgtgct ccgtcttctc accgatgccg gcatttctgc
     2881 agagtcgcag atcatcacca cgtccggtga cgcggtgctt gaccgcggcc tgcaccagat
     2941 cggcggacag ggtgtgttcg tccgcgaact ggacaatgca atcctgcgtg gcgaaatcga
     3001 tgcagcggtg cacagcatga aagacatccc ggcagaacgg cctgagggat tgatcacagc
     3061 agcaattctt ccccgtgatc cgccgtatga tttttttgtg tttaactgtc cggtagagaa
     3121 gatcttctcg gtgggaacgt cgagtacccg ccggcgggca caacttctcc ggtattatca
     3181 ctgtatgccg caggtgcggg ttgaaccgct gcggggaaac gtggacacac ggcttgcaaa
     3241 actgaatgac ggcatgtatg atgcgattgt acttgcagaa gcaggacttg tacgtctcgg
     3301 ctatcatgtg aacggtgtcc ggcttcctgc ggaacatttt gtaccgtccc cgaatcaggg
     3361 aacggttgcg gtggtctgcc gcgacacgcc ggaacttcgc gaggcatttg catccttaaa
     3421 cgatgcccag ac
//
LOCUS       DALTZZ010000117         4440 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_18393,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000117 DALTZZ010000000
VERSION     DALTZZ010000117.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18393"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(28..933)
                     /locus_tag="O0X12_02175"
     CDS             complement(28..933)
                     /locus_tag="O0X12_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HJK53001.1"
                     /translation="MEHAEIITNAYNFTKNAFFSIAQFPRWLVLFFYIIGPVIVGFIL
                     ATIVLQLIVLPILVSLLVGPDFGFTQEVLSGLLQFLALLIGISCIFFVPLMQGYCYRL
                     FRSDDTMPDTGNLWGLFFNGWRINITVLIYAIPLMLISIIYMLVFMYLFPDTGMYSTA
                     DVLDMEGMMYVGTVFSYAAIQFVTTLVVLLFAFIGITHLCRTGSIREAVSLRTITGII
                     SRIGWYDYILSLVVMSILYLMVTFVVVSLSQLFSNNLVIMTVLYVLYIFALVPITVFF
                     IKYLSEVYDTAFLPDERDDEEFDFF"
     gene            1037..1513
                     /locus_tag="O0X12_02180"
     CDS             1037..1513
                     /locus_tag="O0X12_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53002.1"
                     /translation="MGILGQLERALGVWYPDPDADPDDTNPVVSVAFREYVMNRLGSA
                     YSVADREERAVGEPDVVCVRNEDDMRFDIITCFRSRMFVGEDGEAYLPWVTPETYTAY
                     LSYAETERNPCYVIFGLHGFADEPKFLFTVPLAEAVPNLPRTVLQKYEIKKDAALL"
     gene            1517..2611
                     /locus_tag="O0X12_02185"
     CDS             1517..2611
                     /locus_tag="O0X12_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK53003.1"
                     /translation="MKLQIIFRISLFLGAFATMALSNAVVPVLNSIASDPAIQGAVYS
                     AYFLGAFLMVFPAGWMSDRVGRTPLVRIGLCGTLIAAAVLWLSYPDPTAAVLLRFAEG
                     LFTGMFVSAAMAFVNSAADHRKLAGGFVALMNIGMVAGLVVSGWLAAWQAYAGILVFG
                     ILTGVSVLLSLGPADETPALQTVLPVAEVTEIAVYHKWLWAALFVFCGTTGVVISAYP
                     ELSGSSAEMNGVITALMSVATAVLVYAASRMRTADSLSVVRGAGIFLGVSVPIVLVHP
                     VGMIFVGAAFGVITAAILNYLAETKKPQGVMNGMFNMTQYAGMAALPFAAGLLVLPIG
                     YVAVFLIVAVLNAAVGLLVVRCPCYVHHIQ"
     gene            complement(2646..3653)
                     /locus_tag="O0X12_02190"
     CDS             complement(2646..3653)
                     /locus_tag="O0X12_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53004.1"
                     /translation="MMKKSPSLMTRTVLGLCVLLFLAVLVIPVSAGVSQSTIAQGDTL
                     YVSGQAPGSQQVALYFFGPNYFKYDTVQVNDGSYTYQLTTTSDMSPSQYYCVVQSPGT
                     GSRFSVGPLTIGQTTYITVNPGSGVPADGSSFVVKGPNALQGSQAAYALEQMIQSPNI
                     PDLSQTFTFTIAYPTITINPIGTQYMGVPFTVSGTTNLAAGDTLLVSVQIATFWPMGK
                     EEANAEDAESWSQGDSGSVTVQQGASSSQNYWSFSVNPLHSSQYDVTVTGIKTGATAS
                     QEFSVSNQSKPTTVPTTAVTTAVTTTATTVPTPTQSPGFGLAAAAGALGAVVFLTYRR
                     H"
     gene            3778..>4440
                     /locus_tag="O0X12_02195"
     CDS             3778..>4440
                     /locus_tag="O0X12_02195"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HJK53005.1"
                     /translation="MPSKEKDRDNFIVIEELSPQYTVQTTFQPQNRYYPRIPIKPFVK
                     WAGGKGQLLPYIRRAYPKGFGTEITRYAEPFIGGGAVLCDVLSNFSLDEIFISDINAE
                     LINTYTCIRDNCTSLIEILDDWERTYLAASPELRKEIFSAQRDRYNSLKMNDRQDSIL
                     LAALFIFLNKTCFNGLYRVNRKGFFNVPIGAYKKPKICDEANLRNLSKVLQPVTILCG
                     DYR"
BASE COUNT         1186 a         1019 c         1144 g         1091 t
ORIGIN      
        1 gttcagatgt gaacggaaaa attggcgtta gaagaagtcg aactcctcat catccctctc
       61 gtcgggaagg aatgcggtgt cgtaaacctc cgagaggtac ttgatgaaaa agacggtaat
      121 tggcactaac gcaaagatgt agagaacata cagtaccgtc ataataacca ggttgtttga
      181 aaagagctgc gacagcgaca ctaccacaaa cgttaccatc aggtagagga tactcatcac
      241 caccagtgag agaatgtagt cataccagcc gattctgctg ataattccag tgatcgtccg
      301 caggctcacc gcttcacgga tagaacctgt cctgcaaaga tgcgtaatcc cgataaaggc
      361 aaacaacagg acaaccagtg tagtaacaaa ctgaatggca gcataggaga agaccgtccc
      421 cacatacatc attccctcca tgtcaaggac gtcagcagtt gagtacattc ccgtatccgg
      481 gaacaggtac ataaacacca gcatgtagat gattgatatc agcatcagcg gaatcgcata
      541 gataagaacc gtgatgttga tccgccagcc attgaagaac agaccccaga ggttgccggt
      601 gtccggcata gtatcatcgg aacggaacag ccggtaacag tatccctgca tcagcggcac
      661 aaagaaaata caggaaatac ctatcagcag agcaaggaac tgcagaagac ccgaaaggac
      721 ctcttgggta aatccgaagt caggaccaac aaggaggctc accagaatcg gcagaacaat
      781 cagctgcagt acaattgttg cgagaatgaa tcccacaata accgggccga tgatatagaa
      841 gaacagtacc agccaccgtg gaaactgcgc aatgctgaag aaagcatttt tcgtaaaatt
      901 ataggcattc gtgatgatct ctgcgtgttc catgctcgct gatctctctt agaatactat
      961 tatgcagttt tcttataaga taattggcac gctgacgtac tgctttttta agaggacaga
     1021 ggttcaagaa gtaagtatgg gcattttagg ccagcttgaa cgggcacttg gtgtctggta
     1081 ccctgatccc gatgcggatc cggacgacac aaatcctgtc gtgtccgtag cattccgcga
     1141 atatgttatg aaccggctgg gttcggcata ctctgttgcc gatcgtgaag agcgtgccgt
     1201 aggtgagcct gatgttgtct gtgtacgcaa tgaagatgat atgcggtttg atatcatcac
     1261 ctgtttccga agcagaatgt tcgtcggtga ggacggagaa gcctatctgc cgtgggttac
     1321 tccagagacc tataccgcat acctgtccta cgcagaaacc gaaagaaatc cctgttatgt
     1381 catattcggt ctgcacgggt ttgcagacga accaaaattt ctgttcaccg tcccgcttgc
     1441 agaagcggtc cccaatctcc ccagaacagt tttgcagaag tatgagatca aaaaagatgc
     1501 agcccttctc tgattcatga aactgcagat catcttccgt atatcactgt ttctgggtgc
     1561 gtttgcaacg atggcactct ccaacgcggt tgtgccggtt ttaaacagca ttgcatctga
     1621 tcctgcaatc caaggtgcgg tgtactcggc atacttcctc ggtgcatttc tcatggtgtt
     1681 tcccgcagga tggatgtcgg acagagttgg aaggacaccg ctggtacgga taggtctctg
     1741 cggaacactg atagccgcgg ccgtcctgtg gctctcctat ccggatccga cggcggcagt
     1801 tttactccgg tttgcggaag gtctgtttac cggtatgttt gtttctgctg caatggcgtt
     1861 cgtgaactcg gcagcggatc accggaaact cgccggcggg tttgttgcac tgatgaacat
     1921 tgggatggtt gcaggactcg ttgtgagcgg ctggcttgca gcgtggcagg cctatgcagg
     1981 tattctggtg tttggcatac tgacgggtgt ttcggtcctg ctgagcttgg gacctgctga
     2041 cgagacccca gcacttcaga cggttctgcc ggttgcagag gtaacggaga ttgccgtgta
     2101 tcacaagtgg ctgtgggcag cactctttgt gttctgcgga acaaccggcg tggtcatctc
     2161 cgcctatccg gaactctccg gatcatcagc cgagatgaac ggagttatta cagcactgat
     2221 gagtgttgca acggcggtgc ttgtgtatgc cgcatcccga atgcgaaccg cagactccct
     2281 ttccgtagtg agaggcgcag gcatttttct tggggtgtcc gttccgatag tattggtaca
     2341 cccggtcgga atgatctttg tgggagcagc atttggtgtg attactgctg caattttaaa
     2401 ctatcttgcg gagacaaaaa aaccgcaggg agtgatgaac gggatgttca acatgacaca
     2461 gtatgcgggg atggctgcac tgccgtttgc cgcagggctg ctggtcctgc cgatcgggta
     2521 cgttgcggtg tttctgattg ttgcagtact gaatgcagcg gtcgggctgc tggtcgtccg
     2581 ctgtccctgt tatgtacacc acatacaatg aaagacccgt ttgggaacga ccctgaaaaa
     2641 aggagttagt gtcttctgta tgtgaggaaa acaacagcac caagtgcccc tgctgcggca
     2701 gcaagaccaa atcccggtga ctgggttggg gttgggacag tggtcgcagt cgtggttaca
     2761 gcagtcgtta ccgcggttgt tggaacagtg gttggtttac tctggttaga aaccgaaaac
     2821 tcctgcgatg cggtcgcacc ggttttaatg ccggttaccg tgacatcata ctgtgatgag
     2881 tgcagcgggt tgacggagaa gctccagtag ttctggctgc ttgaggctcc ctgctgaacg
     2941 gttacacttc cggaatctcc ctgggaccag gattcagcat cctctgcatt agcttcctct
     3001 tttcccatag gccagaacgt ggcaatctga acgctgacaa gaagtgtatc acctgctgca
     3061 aggttggtgg ttccggaaac cgtgaaaggt actcccatgt actgagttcc tatcgggttg
     3121 atggtaatcg tcggataagc aatcgtaaag gtgaaggtct gggaaagatc cggaatgttc
     3181 gggctttgga tcatctgctc cagagcatat gctgcctgtg aaccctgaag tgcgttggga
     3241 cccttcacaa caaaactgct gccgtcggca ggaaccccgc ttcccgggtt gactgtaatg
     3301 taagtggttt gaccaatggt cagtggacca acactaaacc ggcttccagt acctggactc
     3361 tgaacaacac agtaatactg ggaaggggac atatcgctcg tggttgtgag ctgataggta
     3421 tatgatccgt catttacctg aacagtatca tatttgaagt agttaggacc gaagaagtac
     3481 aaggcaacct gttgggaacc gggtgcctga ccgcttacat aaagcgtgtc tccctgagca
     3541 attgtactct gggaaactcc cgcagagacc ggaatcacga gtactgcaag aaacagaagg
     3601 acacagagac caagaacagt acgggtcatg agggatggag attttttcat catagacaag
     3661 gtatatgtcc tcccgccatc acattaatta taccgttgat gacaaaaatt acatggagga
     3721 accaaaaata gaacgattac ggaacaacag aataaaaagg aaaaattagg attttacatg
     3781 ccatcgaaag aaaaagatag agataatttc attgttattg aagaattatc tccccaatat
     3841 acagttcaga ctacatttca accccaaaat agatactatc caaggattcc gataaaacca
     3901 tttgtaaaat gggcgggagg aaaaggtcag cttcttccat atatccgccg tgcctaccct
     3961 aaaggtttcg ggacagagat tactcgatat gccgagccat tcattggggg aggggcagtt
     4021 ttgtgtgatg tcctgagtaa tttttcattg gatgaaattt tcatttccga tataaatgct
     4081 gagcttatca atacttatac ctgcattaga gataactgta cctctttgat agaaatcctt
     4141 gacgactggg aacggaccta ccttgcagca agcccagaac tgaggaaaga gattttttct
     4201 gcccaaagag acagatacaa cagtcttaaa atgaatgaca ggcaagattc aatattactg
     4261 gccgctctat ttatttttct caataagacc tgtttcaacg ggttataccg ggtaaatcgg
     4321 aaaggtttct tcaatgttcc gatcggggca tataagaaac cgaaaatctg tgacgaagca
     4381 aacctcagaa acctttccaa agtcctgcaa cctgtaacta tcctttgcgg agattacaga
//
LOCUS       DALTZZ010000118         3281 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_18407,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000118 DALTZZ010000000
VERSION     DALTZZ010000118.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18407"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /gene="xseA"
                     /locus_tag="O0X12_02200"
     CDS             <1..783
                     /gene="xseA"
                     /locus_tag="O0X12_02200"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HJK53006.1"
                     /translation="PLPVLLAHTAVQGEGAHIQIAAAIRSLQKKVDVIIVARGGGSFE
                     DLFEFNHPDVVRAIAESEVPVISAVGHETDTTLADYAADIRVPTPSAAAETVVPDRTV
                     LLQQLEEMRRTIRDTVVGRFAAERSTLADLAERVDPSRLSRKLDQMHQQTADFAERIT
                     SALLRRISAEHILCETLRETVAKSARTRITTARLEFLALKEIVVGRDPYKPLELGYAL
                     VWKDGTVVRSAKNISTGDHLSLRLSDGDAVTVVENVTYDRDT"
     gene            767..967
                     /gene="xseB"
                     /locus_tag="O0X12_02205"
     CDS             767..967
                     /gene="xseB"
                     /locus_tag="O0X12_02205"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HJK53007.1"
                     /translation="MTETPEAQTYESMIAELKAIAKQLDDPETPIEEAVRLHQRGLAL
                     IRSCEEFLQTAELTITEVQPEE"
     gene            complement(942..1367)
                     /locus_tag="O0X12_02210"
     CDS             complement(942..1367)
                     /locus_tag="O0X12_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HJK53008.1"
                     /translation="MEIIETIHCRGHKNVRALHKSTFEITKETDLSPNGDCIIAVGAD
                     RGAADLSPAFRDALSRDGTILTTTLSCGGITATVRSEGCPGITLTHGTDLVWRRSTFL
                     CPRTIAIYSDHTACQLPRELIEKLQNEEEMIVTLRAELP"
     gene            1467..2048
                     /locus_tag="O0X12_02215"
     CDS             1467..2048
                     /locus_tag="O0X12_02215"
                     /inference="COORDINATES: protein motif:HMM:NF039705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HJK53009.1"
                     /translation="MDTDMIILDCPVCGEETDFEILKEPPEAVVRCTECGHTMRVTLK
                     EPRIITVKTIVSYGTESHTGTVELMEGDVCSVGDFLVAEVGDESYGVEVMSIERDNAR
                     RAKLPVEEIDVLWTRLVDKVIIRASLNKGAVTIPLYEQVPGDKVYTVDHITSVGGKQF
                     RITRIKLRKGNIIQRKDKTAQAHEIKRIYGERS"
     gene            complement(2076..2522)
                     /locus_tag="O0X12_02220"
     CDS             complement(2076..2522)
                     /locus_tag="O0X12_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HJK53010.1"
                     /translation="MTNEYIIKQLSIFATNTPGTLAEIEKIFQDNGVNISAFCIAEAK
                     NFGVVRAVVNQPDAAFAAFEKKNYLLKFTDVLAVKMSDVPGGLYEVASKFGEAGLNIE
                     YAYASRSAAVPTLVVYVSNPDIPLETAVERILHSGLSLVPAAEMQV"
     gene            complement(2595..3032)
                     /locus_tag="O0X12_02225"
     CDS             complement(2595..3032)
                     /locus_tag="O0X12_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HJK53011.1"
                     /translation="MTRNKYFIKQLSIFSENKSGKLAAIAKVFEECKVNIHAFSIAEA
                     NSFGVVRAIVDKPELAFDAFSKQNYALQYTDVLGIKMNDVPGGLYHVANILGSIGINI
                     EYAYAFRTGTFGTLIVKVSNPDDAVEKILDAGLELVPATDYNF"
     gene            complement(3033..>3281)
                     /locus_tag="O0X12_02230"
     CDS             complement(3033..>3281)
                     /locus_tag="O0X12_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HJK53012.1"
                     /translation="ELTSHFMLEVTRTGPLDDMLVKVELKPDAMTDSINGLIGLQARV
                     ERTLRDALNVSAKVELCPPNSLPRFEGKAKRVVDHRVF"
BASE COUNT          893 a          797 c          802 g          789 t
ORIGIN      
        1 cccctgccgg tccttcttgc gcacaccgca gtacagggag agggggcgca tatccaaatt
       61 gctgcagcaa tcagatcact tcagaaaaaa gtggatgtga tcatcgtggc aaggggagga
      121 ggaagttttg aggatctgtt cgagtttaat catcctgacg tggtgagagc aattgcagag
      181 tcggaagttc cggtaatcag tgccgtcggt catgaaaccg acacaacact tgcagattat
      241 gcagcagata tccgtgttcc aacaccatcg gcagcagcgg agacggttgt cccggatcgg
      301 acagtacttt tgcaacagct ggaagagatg agacgtacaa tccgtgacac tgtagtcggg
      361 agatttgcag cagaacgaag tacgcttgcg gatcttgccg agcgggttga tccttcaaga
      421 ctttcgcgga aactggatca gatgcatcag cagacagcag actttgcgga aagaattacc
      481 tcagctctcc ttcgaagaat ttctgccgag catatattgt gtgaaacgct tcgagaaact
      541 gtggcaaaat ctgcccgaac gaggattaca accgcacgac tagagtttct tgccctaaaa
      601 gagatcgttg ttggaagaga tccgtacaaa ccacttgaac ttggttacgc ccttgtatgg
      661 aaagacggga cagttgtccg ttcggcaaaa aatatcagta ctggagacca cctgtcactc
      721 cggctctcgg atggagatgc ggtcaccgtc gtggagaatg ttacttatga cagagacacc
      781 tgaagctcag acgtatgaat cgatgattgc cgagttaaag gcaattgcaa agcagttgga
      841 tgaccctgaa accccaatcg aggaggcagt gcgcctgcac cagcggggac ttgcactgat
      901 ccgcagttgt gaagagttct tgcaaacagc agagctgaca atcacggaag ttcagcccga
      961 agagtaacga tcatctcttc ctcattctgc aacttctcga tcagctcgcg cggaagctgg
     1021 catgcggtat gatcactgta aatggcgatg gtccggggac aaagaaaagt actgcgtctc
     1081 cagacgagat ccgttccgtg cgtgagcgta ataccaggac acccctcgga tctgacagtc
     1141 gccgttatac cgccgcagga taacgtagtc gtgagaattg ttccgtcgcg agagagtgca
     1201 tcgcggaacg caggagaaag atctgcggca ccacgatcgg caccgaccgc gatgatgcag
     1261 tcaccgttgg gagagagatc tgtttctttg gtgatctcaa aagtgctttt gtgcagtgcc
     1321 cggacatttt tatggccgcg gcagtggata gtttcgatga tctccattgt ttttgctatg
     1381 atcttgttgt gaagggacaa ataaattgtc ttgatgctca catcagcagt agcttaacta
     1441 tctcaaaaag caacatgatt agcacaatgg ataccgatat gataattctt gactgtcccg
     1501 tctgcggtga ggagacggat ttcgaaattc ttaaagaacc acccgaggca gttgtacgct
     1561 gtaccgaatg tggccacacc atgcgggtca cgctgaaaga accaaggatc attaccgtca
     1621 aaacaatcgt cagctacgga acagagtcgc acacgggaac tgttgaactt atggaaggag
     1681 atgtctgttc cgtcggtgat ttccttgtag cagaagtggg tgacgagtcc tacggtgttg
     1741 aggttatgtc catcgaacgc gacaatgccc gaagagcaaa acttcctgta gaagagattg
     1801 atgttctctg gacacggctc gtagataaag tcatcattcg cgcttcctta aacaaaggcg
     1861 cggtaaccat accgctctat gaacaggtac cgggagacaa agtgtacaca gtggatcaca
     1921 tcacctcagt tggcggaaaa cagttccgga ttacccgtat caaactgcgg aaaggaaaca
     1981 ttattcagcg gaaagacaaa actgcccaag cacatgagat caaacggata tacggcgagc
     2041 ggtcctgaaa tctatcactt ttttcaaaaa tatgattata cctgcatctc tgcggcaggc
     2101 acaagggaaa gacctgagtg aagaatcctt tcaacggctg tctctagagg aatatcaggg
     2161 ttggaaacat acacaaccag tgtcggaaca gccgcactgc gtgatgcata tgcatactca
     2221 atattcagac cagcttcacc gaactttgat gcaacctcat acaggccgcc aggtacatcc
     2281 gacatcttca ctgcaagaac gtccgtaaac ttcagcagat agtttttctt ctcaaatgcc
     2341 gcaaacgcag catccggctg attcacaacc gcccggacca ctccgaagtt cttcgcttct
     2401 gcaatgcaga acgctgagat gttcaccccg ttgtcctgga atatcttttc aatttctgcc
     2461 agggtccccg gcgtattcgt agcaaagatc gaaagttgtt tgataatata ttcatttgtc
     2521 atgacgcaat cactcctact gtctatctcc cactggatag cgtattaagt ttttgatgta
     2581 ctcaaaaaaa gaaattagaa gttataatct gtggccggaa caagttcgag tcctgcgtcg
     2641 aggatcttct ccactgcatc atcgggattt gataccttca cgatcagggt accaaacgtt
     2701 cctgttctga atgcatatgc atactcgata ttgatgccga tactgcctaa aatatttgcc
     2761 acatgataca gaccaccggg aacatcattc attttgattc ccagaacatc agtgtactgc
     2821 agagcatagt tctgttttga gaatgcatca aaggcaagtt ccggcttatc gacaatcgcc
     2881 cgcaccacac cgaagctgtt cgcctcggca atgctgaacg catgaatatt gaccttacac
     2941 tcctcaaaga ccttggcaat tgctgcaagt ttcccggact tgttctcgga gaagatcgaa
     3001 agctgtttga tgaagtactt atttcgcgtc atttagaaca cccggtgatc cacaacacgt
     3061 tttgcttttc cttcaaaccg cgggagagag tttggcggac acagctcaac ttttgcggag
     3121 acgttcagag cgtcacgcag ggtccgctca actctcgcct gaaggccaat caggccgttg
     3181 atactgtcag tcatcgcatc cggttttaac tcaaccttca ccagcatatc atcaagcggt
     3241 ccggtacggg taacttccag catgaaatgg cttgtcaact c
//
LOCUS       DALTZZ010000119         2854 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_18479,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000119 DALTZZ010000000
VERSION     DALTZZ010000119.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18479"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="O0X12_02235"
     CDS             <1..237
                     /locus_tag="O0X12_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HJK53013.1"
                     /translation="RKYCLDNAEVEVVRPMHNLEFCANCNRLRVTSDGKLKPCLLRTG
                     NEVDIRGLHGEDLKAAIAKAVQNRAPYFTPAPKV"
     gene            332..1834
                     /locus_tag="O0X12_02240"
     CDS             332..1834
                     /locus_tag="O0X12_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HJK53014.1"
                     /translation="MNDSEVLMNPNELVQFLKKNPADFTKDDIIRFCEENGIEMVNFR
                     YVAGDGKLKTLNFVISSKEYLDTILSDGERVDGSNIFPFIEAGSSDLYVVPRYGTAFV
                     DPFAEVPTLDILCSYYDNEGTPLASSPEYILRCARDTFKKNTGMDFKCLGELEYYVIC
                     PEEDLYPGLDQKGYHASGPFCKFEEMRTEAMLMVARAGGQIKYGHSEVGCFTKDGYYY
                     EQHEIEFLPTDPILAVEQIVIAKWVLRMLGYKYGVEVSYAPKITVGKAGSGLHFHMLA
                     EKNGKNMMVKDGQLSDIAKRMIAGILDVGDAVTAFGNPIPTSYLRLVPHQEAPTYICW
                     GDRNRSVVVRVPLGWTGSTDMAAEANFGIRRKVNKASKQTFEYRVADGSADIYETVAC
                     LIIGAMHGLAMPDALEQAQKLYASGNIFNAEYAAFLKTLRQLPTCCDESADVLLAKRA
                     LFEADGIFPAGVIDAQAKKLKAFGDKGLSDSLLGNTDAFAELVARYLHVA"
     gene            1860..>2854
                     /locus_tag="O0X12_02245"
     CDS             1860..>2854
                     /locus_tag="O0X12_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53015.1"
                     /translation="MQRAKNVQYPVISPSSTEGNVCVRLTFPFRHLHHTFTIAVSRAL
                     YDGARTSGKTARVPARTGDAWLAGYYTAFTTDPALVPFYQTILAKLRKIRSSQNYDDD
                     SYLELIAAFVQSIPYDTEKVASINRAPRFPVETVVDKKGICSDKSLLLAGLLAHEGYA
                     VAVLHFSAENHLAVGIPVQDGYDFRGCGCAVIESTALGYVGDAEGVFPAGDGKTRKLA
                     SRPKVFFVGHGSKRYGSISQVARILATRSALLEKLAEDGPFTQQIRSKESSVSLIKEE
                     LSVSRNKIEMLEENTKKLAEDTEGFAVAYTEYREAIMCHNKRVTLLAEEIQRYNQL"
BASE COUNT          763 a          702 c          721 g          668 t
ORIGIN      
        1 agaaaatact gcctggacaa cgccgaggta gaagtcgtac ggccaatgca caatcttgag
       61 ttctgtgcaa actgcaaccg cctccgggta acctcggacg gtaaactcaa gccatgcctt
      121 ctccgtaccg gaaatgaagt ggatatccgg ggactgcacg gagaggactt aaaggcagca
      181 attgccaagg cagtacagaa ccgtgctcct tacttcactc ctgcacccaa agtctgaaac
      241 ttctgccaag tacctttttt cctttgatat acatcatgtc acacaatcgc atggttgggt
      301 atttatgccg tcccttcgaa tagttacggc aatgaatgat tcagaagtac tgatgaatcc
      361 taacgagctg gtacagttcc tgaagaaaaa ccctgctgat tttacgaagg acgatatcat
      421 ccggttctgc gaggaaaacg gaatcgagat ggtgaacttc cgctacgttg caggcgatgg
      481 aaaactcaag acactgaatt ttgtaatctc ttccaaagaa tacttagaca ctatcctctc
      541 ggacggcgag cgcgttgacg gcagtaacat cttcccgttc atcgaggccg gcagttccga
      601 tctgtatgtt gtaccgcgct acggcacagc attcgtagac ccgtttgcag aggtgccgac
      661 gcttgacatt ctctgttcct actatgacaa tgaaggtacg cctcttgcat cctctccaga
      721 gtacatcctg cgctgtgcgc gcgatacgtt taagaaaaac accggaatgg acttcaaatg
      781 tctgggtgaa cttgagtatt atgtaatctg ccccgaggag gatctgtatc ctggccttga
      841 tcagaaaggc tatcatgcat ccggtccgtt ctgcaaattt gaggagatgc gtaccgaagc
      901 tatgctgatg gttgcacgcg ccgggggaca gatcaagtac ggtcactctg aagtaggctg
      961 cttcaccaag gacggctact actacgagca gcacgagatt gagttcctgc cgaccgatcc
     1021 aattcttgcc gttgagcaga ttgtgatcgc aaaatgggta ctgcggatgc ttggttacaa
     1081 gtacggcgtc gaggtttcct atgcaccgaa gatcaccgtc ggcaaggcag gatcaggtct
     1141 gcacttccac atgcttgcag aaaagaacgg caagaacatg atggtaaaag acggtcagct
     1201 ttccgacata gcaaagagaa tgattgcagg tattctggac gtcggtgatg cggttaccgc
     1261 attcggcaac ccgattccga cctcttacct gcggctggtt ccgcaccagg aagcaccgac
     1321 ttacatctgc tggggagatc ggaaccggtc ggttgttgtc cgtgtcccgc tgggatggac
     1381 tggttctaca gacatggcag cggaggcaaa ctttggtatc cgccgcaagg tgaacaaagc
     1441 atccaaacag acgttcgagt accgcgtggc tgacggatcg gctgacatct acgagaccgt
     1501 tgcctgcctg attatcgggg caatgcatgg tcttgcaatg ccggacgcac ttgagcaggc
     1561 acagaaactg tatgcaagcg gcaacatttt caatgccgag tatgcagcct tcttgaagac
     1621 gctccgccag ctgccgacct gctgtgacga atctgcagat gtacttcttg caaaacgcgc
     1681 actctttgaa gcggacggca tcttcccagc aggagtgatt gatgcacagg caaagaaact
     1741 gaaagcattc ggtgacaagg gattatccga cagtctgctc ggtaatacag acgcattcgc
     1801 cgagcttgta gcacgctacc tccacgtggc ataatccctt ctcttttttt ttgaaaaata
     1861 tgcagcgtgc gaaaaatgta cagtacccgg taatcagccc cagcagtaca gaaggcaatg
     1921 tttgcgtcag gctcacattt ccgtttcgcc acctgcacca tacttttacg attgcagtat
     1981 ccagagcact ctatgatgga gcgcgcacat ccggaaaaac agcacgtgtc cctgcaagaa
     2041 caggagatgc gtggcttgca ggatactata cagcattcac cactgatccg gcacttgtcc
     2101 cattttacca aacgatactt gcaaaactgc ggaagatccg ttcatcgcag aactatgatg
     2161 atgattcata ccttgagcta attgcagcat ttgtacagtc catcccgtat gatacagaga
     2221 aggttgcctc aattaaccgc gcaccgcggt ttcctgtgga aacagtagtg gacaaaaaag
     2281 gcatatgcag cgataaatca ctattgcttg caggactcct ggcacatgaa ggatatgcag
     2341 ttgcggtact gcacttttcg gcagaaaatc atcttgcagt aggaattccc gtgcaggatg
     2401 gatacgactt tcgcggctgc gggtgtgccg tgatcgaatc aacggccctc gggtatgttg
     2461 gagatgcaga aggagtgttc ccggcaggag atggaaaaac cagaaaactt gcatcccgtc
     2521 cgaaggtatt tttcgtagga cacggttcaa aacggtatgg aagtatttct caagttgcta
     2581 gaattcttgc aacgcggtca gccctgctgg aaaaacttgc agaagacgga ccgtttacac
     2641 agcagatccg atccaaagag tcatctgttt ccctcatcaa agaggaactt tcagtatcac
     2701 gaaacaaaat tgagatgctt gaagagaaca cgaaaaagct tgctgaagac actgagggtt
     2761 ttgctgttgc atatacagag tacagggaag caatcatgtg ccataataag agagtgacct
     2821 tacttgcaga ggagattcag cgatacaacc agct
//
LOCUS       DALTZZ010000120         2077 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_18643,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000120 DALTZZ010000000
VERSION     DALTZZ010000120.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18643"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /gene="purH"
                     /locus_tag="O0X12_02250"
     CDS             <1..675
                     /gene="purH"
                     /locus_tag="O0X12_02250"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843907.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HJK53016.1"
                     /translation="TQLESYIKAREVDPVSAYGSVVAMNSPVGKDVAEEICSTFVEVL
                     IAPSFTDEAREIMKRKENMRLLILPPEVPADELRTIDGGVLVQRTPAYEEGWKVVSKR
                     APTEAEAEAMKFAWKIVKYAKSNAIVYANATELVGIGVGQMNRVDSAKIAVMKAAEFG
                     RTMKDTVVASDAFLPFPDTLEVAAAAGATALIQPGGSIRDAEVIAKADELGVAVVFTG
                     TRHFRH"
     gene            757..1623
                     /locus_tag="O0X12_02255"
     CDS             757..1623
                     /locus_tag="O0X12_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HJK53017.1"
                     /translation="MAIGIVENLRSSYDYTNKSLNSFTDWVILITITMLMLAGVVLVV
                     LGILGMAVTSITTVTPDAVTVTQVPLFDPVGATTVTGTETTVSGSVPLAELFGGAFSL
                     VAMGIGAVLSLVFGILWTGFILRVYRGGELKLGSWGRMFLDGLLATIISLLYCIPYVV
                     ICILLAFGPMTNPTYALIALIVEFIVILITSMVLLMGLIRFAKEQRFGAAFQIKELFS
                     IIGTIGWLRYLANIIVIGIIMLVISVILLLILGIGWVLLLVLVPFLSIWEAKFFANLY
                     ESALPAPAAVEE"
     gene            1735..>2077
                     /locus_tag="O0X12_02260"
     CDS             1735..>2077
                     /locus_tag="O0X12_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HJK53018.1"
                     /translation="MFLPVSVTENLGRSFQYAKDTLCRTWIEYVIVFVCALIPVVNLL
                     TLPGFVVRVFRNDFALGNWLMLVIDSVKALIITVVYLIIPTVISVVFGAVLGPRNIVT
                     LAVSFVVCLVFV"
BASE COUNT          450 a          474 c          574 g          579 t
ORIGIN      
        1 acccagcttg agtcctacat caaggcccgc gaggtagatc cggtctctgc ctacggctcg
       61 gttgttgcga tgaacagtcc ggtcggaaag gatgtggcag aggagatctg ttccacgttt
      121 gtggaggtgc tgattgcccc atccttcacc gatgaagcac gcgaaattat gaagagaaag
      181 gagaacatgc gtctcctgat cctcccgccc gaggttccgg cagatgagct gcggacgatc
      241 gacggcggtg tgctggtgca gagaacaccg gcatatgaag aagggtggaa ggttgtctcc
      301 aaacgtgcac cgaccgaggc tgaagcggaa gccatgaaat ttgcctggaa gattgtgaag
      361 tatgcaaaga gcaatgcgat tgtgtacgca aacgcaaccg agctggtggg tatcggtgtc
      421 ggccagatga accgtgtgga ctctgcaaag attgcggtga tgaaggccgc agagtttggc
      481 agaacaatga aggacacggt tgtggcatcg gatgcgttcc tgccgttccc ggacacgctg
      541 gaggttgccg cggctgccgg agcaaccgca ctgattcagc cgggcggatc gatccgtgac
      601 gccgaagtta ttgcaaaggc agatgagctg ggagtggccg tggtgttcac cggaacccgg
      661 catttccgcc actaattttc ttttttccga tttacacaaa ctatatatgc cgttgggaac
      721 aactttcgat attcccaaaa agaggtgtat acatcaatgg ctattggtat tgtagaaaat
      781 ttgcgtagtt cctatgacta cacaaataag tcactaaact ctttcaccga ttgggtaatc
      841 ctgatcacca tcaccatgct gatgctggca ggtgtggtct tggtggtcct gggtatcctc
      901 ggtatggctg taacctcaat cacaacagtt actccggatg cagtaaccgt gacacaggtt
      961 ccgctctttg atccagtagg agcaacaacc gtcaccggaa ctgaaacaac agtgtcagga
     1021 agtgttccgt tggctgaact gttcggcggg gcattttcac tggttgccat gggtatcgga
     1081 gcagtacttt ccctggtctt tggtattctc tggaccggat ttatcctccg tgtgtaccgc
     1141 ggcggtgaac tgaagcttgg atcgtggggc agaatgttcc ttgacggtct gctcgcaacg
     1201 atcatctcgc ttctgtactg tatcccgtat gtggtaatct gtatcctgct ggcgttcggc
     1261 ccgatgacca acccgacgta cgctcttatc gccctgattg tagagtttat agtgatactg
     1321 atcacctcca tggtactgct catgggactg atccggtttg caaaggaaca gcggttcggt
     1381 gcagcattcc agataaagga gctgttcagc atcattggca caatcggatg gctgcggtat
     1441 ttagcaaaca ttatcgtgat tggcattatt atgctggtca tcagcgtaat tctgctgctg
     1501 attctcggaa ttggatgggt gctgctgctt gttctggtac cattcctgag catctgggag
     1561 gcaaagttct tcgccaacct gtatgagtca gcacttcctg cacccgcagc tgttgaggaa
     1621 taaaatcctc atttttgttt ttggcatttt ttgtaaatga tttttgttta cttccccctg
     1681 ttgcggcaac tatatatctg gcacggcaga atgttttcta cgtaatcgag gtggatgttt
     1741 ttgccggtat ctgtaaccga aaatcttggc aggtcctttc agtatgcaaa ggatacgctg
     1801 tgcagaactt ggatagagta tgtgattgtg tttgtgtgtg cactgattcc ggttgtgaat
     1861 ctgctgacac tgccggggtt tgtggtccgt gtgttccgga atgattttgc cctgggaaac
     1921 tggctgatgc tcgtgatcga cagtgtgaag gcattgatca tcaccgttgt gtatctgatt
     1981 attcctacag tcatctccgt tgtattcgga gccgttcttg gaccgcggaa tattgttaca
     2041 cttgccgttt cctttgtggt ctgtctggtg tttgtac
//
LOCUS       DALTZZ010000121         3830 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_18849,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000121 DALTZZ010000000
VERSION     DALTZZ010000121.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18849"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="O0X12_02265"
     CDS             complement(<1..181)
                     /locus_tag="O0X12_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HJK53019.1"
                     /translation="MPSGSRSDLTEPPFYLPQFEQAYRYIIDEYLVQPRETAIFIPCA
                     VRKPYSQSPSHKLFHG"
     gene            complement(188..445)
                     /locus_tag="O0X12_02270"
     CDS             complement(188..445)
                     /locus_tag="O0X12_02270"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HJK53020.1"
                     /translation="MEKPLYRSANDRWLAGVCGGIAEYAKIPSILVRLIFIALCLVLL
                     PFWTIILILFYIVASFYLPEAPSRKTIEDPNVIDAEFEVKE"
     gene            481..1056
                     /locus_tag="O0X12_02275"
     CDS             481..1056
                     /locus_tag="O0X12_02275"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HJK53021.1"
                     /translation="MKRELPAYAVLPPCPVTVVCTYDADEKPNGLAVSWTGIAVSEPP
                     HVMIAVRPGRYSHAALCERGEFTMNIPSEDQMCQADYFGITSGRKTNKFEDLDLTPMP
                     AEHVHAPMIAEFPLSLECRVVSRQEIGSHSVFIAEILAVHADERILDAEGKIDVVLLR
                     PLAYDHAKYLYLAQGQVLGRAFSAGKILKKS"
     gene            complement(1148..1588)
                     /locus_tag="O0X12_02280"
     CDS             complement(1148..1588)
                     /locus_tag="O0X12_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJK53022.1"
                     /translation="MSGMSVTNGNTIRVHYTGTLTDGTQFDSSEGRDPLEFVVGSGMV
                     IPGFDKGVVGMEIGETRTLEIPAKDAYGPRSDDMTVAIPRSEFGSDFTAEIGEQLLIQ
                     LGDGNQIPVTITQIDDKTVTLDANHKLAGKDLIFTVTLVEIVAE"
     gene            complement(1679..3193)
                     /gene="rpoA2"
                     /locus_tag="O0X12_02285"
     CDS             complement(1679..3193)
                     /gene="rpoA2"
                     /locus_tag="O0X12_02285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HJK53023.1"
                     /translation="MDPVEEEDRFGDSLEDAVIGEYEAGIPESEEEVEEIEEAAESED
                     ESDAENAAKSRKGKKAAGAEEEIPESEIAKLLHTWDLPKTLFDQIRVWLEAKTPEEME
                     TVDLDRLKTRVCRLVDEDMPVSTTTELTKILIAKHDAGHDVTNEQFEEIVSRINAEYE
                     RTRVQPCEAVGINAAHSIGEPGTQMTMRTFHYAGVAEINVTLGLPRLIEIMDARKEPS
                     TPTMTIFLEKGYREDRDKAREVSWKIEAAPLHEFGDIETDIADMCVIVQINREVCKKR
                     KISVAEILERAPQKIKDKRHYRDFEVEVDEKEAILRFLPKNEESYQNLFQLAEHVRQV
                     IVQGIDDIRRVVVRKENDEYILHTEGSNLKDVFEIDGVDCTRTRTNNIAEIANTLGIE
                     AARSAIIYEAFSTLKEQGISVDLRHIMLVADIMCMDGEVKQIGRHGIAGEKESVLSRA
                     SFEVTVNHLLDAAVAHEFDELSGVTENVIVGQPILLGTGDVKLMVRRVNPTKSA"
     gene            complement(3274..>3830)
                     /locus_tag="O0X12_02290"
     CDS             complement(3274..>3830)
                     /locus_tag="O0X12_02290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313770.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A'"
                     /protein_id="HJK53024.1"
                     /translation="EMQVMKELGKARDRTGEIASRHLGFENSAVVMAVSGARGSMLNM
                     AQMAGCIGQQAVRGERIVRGYEDRTLPHFKRGDKGSDAHGFVRNSYKSGLTPTEFFFH
                     AIGGREGLVDTAVRTSQSGYLQRRMINALQDLKVAYDGTVRSTGGKIIQFKYGEDGTD
                     PAKSASGLPVDVKGIAESVLKEAV"
BASE COUNT          942 a         1034 c          924 g          930 t
ORIGIN      
        1 tgccatgaaa cagtttgtgg ctcgggcttt gactgtaagg ttttcgaaca gcacacggaa
       61 taaaaatcgc ggtttcgcgc ggctgcacca aatattcatc gatgatgtag cggtaggcct
      121 gctcgaactg cggaagataa aacggtggtt cggtaagatc actgcgggag ccggacggca
      181 tgcgggatta ctcctttacc tcgaactctg catcgataac attgggatct tcaatggttt
      241 ttcgcgaagg tgcttcaggg agatagaagg atgccacaat gtaaaacagg atcagaatga
      301 tggtccagaa cgggagcaga acaaggcaca aggcaataaa gatcagcctg acaagtattg
      361 acggaatttt tgcgtattca gcaatgcctc cgcagactcc tgcaagccag cggtcgtttg
      421 cagaacggta gagaggtttt tccatactaa catattgtcc tgctgcaaaa atatagttgt
      481 atgaagcgtg agttgcctgc ctatgcggtt ctgcctccat gcccggtaac cgttgtctgt
      541 acgtatgatg ccgatgaaaa accaaacggc cttgcagtgt catggacagg gattgccgtg
      601 tccgaaccgc cgcatgtgat gattgccgtg cgtcccggcc gctacagtca tgcagcactc
      661 tgtgagcggg gagagttcac aatgaatatc ccctcagagg atcagatgtg ccaagcggat
      721 tatttcggaa tcaccagcgg cagaaagacg aataaattcg aggatctcga tctgacgccg
      781 atgcctgctg agcacgtgca cgcccccatg attgcagagt tcccgctctc tcttgaatgc
      841 cgagtcgtct cacgacagga gatcggatca cactcggtgt ttattgcaga gattctcgca
      901 gtacatgccg acgagcgcat tctggacgcg gaagggaaga tcgatgtggt gttactgcgg
      961 cctctggcat atgatcacgc aaaatatctt tatctcgcgc aggggcaggt actgggacgg
     1021 gcattttccg cgggaaagat actgaaaaaa tcatgacagg atctgtaact cacgaaaaaa
     1081 acggatgagg ttgacaggat gaaatgaatt gtcttcatca cccggaaaaa tatttttgaa
     1141 ggatatctta ttctgcaacg atctccacaa gcgtcacggt aaagatcaga tcctttcctg
     1201 cgagcttgtg gtttgcatca agcgttacgg ttttgtcatc aatctgggta atggtaacag
     1261 gaatctgatt tccgtcaccc agctggatta acagctgctc accgatctct gcggtaaagt
     1321 cagaaccaaa ctcgctgcgc gggatggcga cggtcatatc atcagatctt ggcccatagg
     1381 catcctttgc agggatctcc agagtacggg tctcccctat ttccataccg acaacacctt
     1441 tgtcgaatcc ggggatcacc atgccgctac cgacaacaaa ctccagcggg tcacggccct
     1501 ccgaagagtc aaactgtgta ccgtcggtga gagttccggt ataatgaacg cgtatcgtat
     1561 tgccgtttgt aacagacata ccgctcatgt gggttgtgcg aagttaagta atttcggaaa
     1621 agaaaccgtt tcgcgtgaac gctacacgac aggaaaacat caaaaaagaa aagaagtatt
     1681 atgctgattt cgtcgggtta acccggcgaa ccatcagctt cacatcgcct gtaccgagaa
     1741 gaatcggctg accaacgata acgttctccg tcacaccgga cagctcatca aactcatggg
     1801 caactgcggc gtccagcagg tggttaactg taacctcgaa ggatgcacgg gagagaaccg
     1861 actctttttc tcctgcaata ccgtgacgac cgatctgttt gacctcacca tccatgcaca
     1921 taatatctgc aacaagcatg atgtggcgga gatccacaga gataccctgt tccttcaggg
     1981 ttgagaatgc ctcatagata attgcggaac gtgctgcctc aataccaagc gtgtttgcaa
     2041 tctctgcaat gttgtttgta cgcgttctgg tacagtccac accatcaatc tcaaagacgt
     2101 ctttcaggtt agaaccttcc gtatggagaa tgtactcatc attctccttt ctgaccacca
     2161 cacggcggat atcatcaata ccctgcacaa ttacctggcg gacatgctcg gcaagctgga
     2221 acaggttctg gtatgactcc tcgtttttcg ggaggaaacg gaggatagcc tccttctcgt
     2281 caacctccac ctcgaaatca cggtagtggc gcttgtcttt aatcttctgc ggagcgcgct
     2341 ctaagatctc ggcaacggag atctttctct tcttgcacac ctcgcggttg atctgcacaa
     2401 tcacacacat atccgcaatg tccgtctcga tatcaccgaa ctcgtgcagc ggagcagcct
     2461 cgatcttcca tgaaacctca cgggccttgt cacgatcctc acggtacccc ttctcaagga
     2521 agatggtcat cgtcggtgtg gacggttcct tgcgtgcgtc cataatctca atgagacgcg
     2581 gcagacctaa ggtaacgtta atttcagcca cacccgcata gtggaacgta cgcatcgtca
     2641 tctgcgtacc cggttcaccg atggagtgag cggcattaat acccacagct tcgcacggct
     2701 gaacacgggt acgttcatac tccgcattga tacgggacac gatctcttcg aactgttcat
     2761 tcgtgacatc atgtcctgcg tcgtgcttgg cgatcaggat cttcgtcagc tcggtcgtgg
     2821 ttgagaccgg catgtcctca tccaccagac ggcagacacg ggtctttaac cggtcaagat
     2881 caacagtctc catctcctca ggagtcttgg cctcaagcca gacacggatc tgatcaaaca
     2941 gggtttttgg aagatcccag gtgtgcagaa gctttgcaat ctcgctttca ggaatctcct
     3001 cctcggcacc tgcggctttc tttcctttgc gggattttgc tgcattctct gcgtcggact
     3061 catcctcgga ctctgctgcc tcttcgatct cctcgacttc ctcttccgac tcaggaatac
     3121 ctgcctcgta ctcaccaatc actgcgtctt ccagagagtc accaaaacgg tcttcctcct
     3181 cgaccggatc catgagaaca tcctcctcaa taaaggaagg ttctgcatac ggtgccattt
     3241 catcatcaga ctttttcttt cttggcgcca tagttacaca gcctccttta acacggactc
     3301 agcaataccc ttcacatcaa ccggtagacc agatgcggat ttagccggat ccgttccatc
     3361 ttcaccatac ttgaactgaa tgatcttgcc tccggtactg cggacagtac cgtcgtatgc
     3421 gaccttcaga tcctgcagag cattaatcat acgccgctgg agataaccgg actgggatgt
     3481 acggactgca gtatccacaa gaccttcacg accaccgatt gcatggaaga agaactcggt
     3541 tggcgttaaa ccggacttgt aggagttgcg gacgaaaccg tgtgcatccg atcctttgtc
     3601 accgcgtttg aagtgcggca gagtacggtc ctcgtatcca cgaacgatac gctcaccacg
     3661 cactgcctgc tggccgatac aaccggccat ctgtgccatg ttcaacatag aaccacgggc
     3721 acctgacaca gccatcacca ctgcggagtt ctcgaatcca aggtgacggg atgcaatttc
     3781 tccggtacgg tcacgagcct tacccagttc cttcatcacc tgcatttcaa
//
LOCUS       DALTZZ010000122         3852 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_18924,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000122 DALTZZ010000000
VERSION     DALTZZ010000122.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18924"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..2487
                     /locus_tag="O0X12_02295"
     CDS             <1..2487
                     /locus_tag="O0X12_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HJK53025.1"
                     /translation="SDARTKLFARLGISETEDSTFAEQRKRCDLWSSDVDGVVLWSKF
                     LTYTDALGETVAAPLLELLFADKLGPGELVPAFLTGYADSLLREAMNERPLLARFSQI
                     PHEQKIAAFAACDRDAISENCKRIIKILDEKIPELYTGASRDSEMGVLTGEFNRKRGH
                     MSIRMLMSKAGGLVQQIKPCFMMSPLSVAQYLDPRSIQFDVIIFDEASQVRPEDALGA
                     LMRGRQLVVMGDSRQLPPTTFFDQIASSDEEDEVESVASITDMESLLNVCKQSYETKR
                     LRWHYRSRHESLIAVSNQEFYDGNLMVFPSPMHETPDLGLSFVYLPDTVYERGKTGVN
                     RGEARAVAEAVSEYYRRFPDKTLGVATFSTRQQEVIRHEVELLLRENPDVESLMRPEN
                     GENFFVKNLETVQGDERDTMLISIGYGFDENHKLSRNFGPLNQDGGERRLNVLITRAR
                     ERCVVFANFRGSDLAVEPGSASGISALATFLTYAADRSTPLGAGGEAPDDVAGLFGDT
                     IARLLEDNGYHVAQNVGCAGFRIDIAIEDPNEPGVYLAGILCDGPYYWSSEVARDRDR
                     LRAQVLEGLGWNLIRIWATEWYQHPASCTKTLLDAVEAAKSAPKEKPAPKIISPEKPS
                     AKKPSAKKTEEEPAASAPPASSAAPVSLSLVPYTCCSECSLDSYHQFASVPDSVLGTA
                     IVQIVAIEGPISPSVLAARVKELGRVPRMTAAVRNRIASAAETEVSEGRLSVDEEGFL
                     TVPECVISPRERPAKWSAADISLAEISAAAVSVLEKQFSTPRDDLIRQTAVVLGFKAT
                     AAVKERIASGIDAGISDGRILLEGNICKAA"
     gene            2556..3242
                     /locus_tag="O0X12_02300"
     CDS             2556..3242
                     /locus_tag="O0X12_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HJK53026.1"
                     /translation="MSEAVSAVLTCAPYLLEGMCVTLALVALALLIGFLCGIVLTIGQ
                     VYGPRYIKRLVDIYVWFFRGLPNIVLLFLFYFALFPMTGFDVPAFAVAVVVLGLRSAA
                     YQSQIFRGAIESIADGQMLAARSLGMTKWQSIRTVILPQALRLSLPGWSNEYPVLLTD
                     SAVAYVIGVAELLTRTAQVISKTGEPMVLYLTCAVIFIILNYGGMMLIQRVEKKVRIP
                     GFGNNEVTET"
     gene            3239..>3852
                     /locus_tag="O0X12_02305"
     CDS             3239..>3852
                     /locus_tag="O0X12_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HJK53027.1"
                     /translation="MSDSPILRVENLSKSYGDLEVLKSVSFDVYKGETKAFIGPSGTG
                     KSTLLRCINQLTVPDDGHVYLNGEEITHSGSKINEYRQKMGMVFQNFNLFDHLTAVRN
                     VEIALLKVKKMTKEEARKKAMFELERVGMADRADYYPAQLSGGQAQRVSIARALAMDP
                     DVMLFDEPTSALDPELKREVLEVMRKLAADGMAMLIVTHEMHFAT"
BASE COUNT          820 a         1037 c         1028 g          967 t
ORIGIN      
        1 agtgatgccc gcacaaaact ctttgcccgt cttggtatct ccgagactga ggattcaacg
       61 tttgccgagc agcgcaaacg ctgcgatctc tggtcatcgg atgttgacgg tgttgttctg
      121 tggagtaaat tcctcaccta taccgatgcc cttggagaaa cggttgcggc cccgcttctg
      181 gagctgctct tcgcagataa acttgggcct ggtgaacttg ttcctgcctt ccttaccggc
      241 tatgccgact ccctgcttcg cgaagccatg aatgagcgtc cgcttcttgc acgcttctcc
      301 cagattccgc acgaacaaaa aattgcggca tttgccgcct gtgatcgtga tgcaatctcg
      361 gaaaactgca aacgcatcat aaaaattctc gatgaaaaaa taccggagct ctacaccggt
      421 gcatcccgcg actccgagat gggtgttctc accggtgagt tcaacagaaa gcgcggtcac
      481 atgtcgattc gtatgctcat gagcaaagcc ggtggtctgg ttcagcagat caagccgtgc
      541 tttatgatga gccccctttc cgttgcccag tatcttgatc cccgttcgat acagtttgat
      601 gttatcatct ttgatgaggc aagtcaggtc agaccggagg atgcactggg agcactcatg
      661 cgtggacgtc agcttgtggt tatgggtgat tcccgtcagc ttccgccgac caccttcttt
      721 gatcagatcg catcctctga cgaggaggac gaggttgagt cggttgcaag catcactgat
      781 atggagagtc tgctgaatgt ctgtaagcag tcttacgaga cgaaacgtct gcgctggcac
      841 taccgctcac ggcacgagtc attgattgcg gtctccaacc aggagttcta tgacggtaac
      901 ctcatggtct tcccgtcccc aatgcatgag acgcctgatc tcggtctctc ctttgtttat
      961 ctgccggata cggtgtatga acgcggtaag acaggcgtca accgcggaga ggcacgggcg
     1021 gttgccgagg cggttagtga gtactaccgt agattccctg ataagacact gggtgttgcc
     1081 accttctcta cccgtcagca ggaagttatc cgtcatgagg ttgagcttct gctccgcgaa
     1141 aatccggatg ttgagtctct gatgcgtccg gaaaatggcg agaacttctt tgtcaagaac
     1201 ctggaaaccg tgcagggaga tgagcgtgac acgatgctta tcagtatcgg gtacgggttt
     1261 gacgaaaacc acaaactgag cagaaacttt ggtcctctca atcaggacgg cggtgagcgg
     1321 cggctgaatg ttttgatcac ccgtgcccgt gaacgctgtg tggtgtttgc caacttccgg
     1381 ggatctgatc ttgctgttga gcctgggtct gcctccggaa tttccgctct tgcaacattc
     1441 ctgacctatg ctgctgatcg cagtactccg cttggcgcag gtggggaagc tcctgatgat
     1501 gtggccggac tgttcggtga caccatcgcg cggctgctgg aggacaacgg ttaccatgtt
     1561 gcgcaaaatg tcggatgtgc cggattccgg attgacattg caatagaaga tccgaatgag
     1621 cctggcgtgt accttgccgg tattctctgc gacggcccgt actactggtc ctcggaggtt
     1681 gcccgcgatc gggaccggct ccgagcacag gttctggagg gactcggctg gaatcttatc
     1741 cgcatctggg caaccgagtg gtaccagcac cccgcatcct gtaccaaaac actgcttgat
     1801 gcagttgagg ctgcaaaatc tgcacccaag gaaaaacctg ctccaaaaat tatctctccc
     1861 gaaaagccat ccgcaaagaa accatctgcg aaaaaaactg aggaagaacc tgcggcttct
     1921 gcaccacccg catcgtctgc cgcaccggtt agtctcagtc ttgtacctta cacctgttgc
     1981 agcgaatgct cccttgacag ttaccatcag ttcgcctctg tacctgactc ggtccttgga
     2041 accgccatcg ttcagatcgt cgcaattgaa ggtcccatct ccccgtctgt tcttgcagcc
     2101 cgggttaagg aactgggccg cgtgccccgc atgaccgcag cggttcggaa ccgtattgct
     2161 tccgctgctg aaacagaggt ttccgaggga agactttccg tggatgagga aggattcctg
     2221 accgttcccg agtgcgttat ttctccgcgt gaacgaccag ccaagtggtc tgctgcagac
     2281 atttcccttg ccgagatctc tgctgcggcc gtttcggttc tggaaaaaca gttctccact
     2341 ccgcgtgatg atctcatccg acagacggct gttgttctcg gcttcaaagc aaccgctgcg
     2401 gttaaagaac ggattgcatc aggtattgac gcaggtattt ccgatggccg tatccttcta
     2461 gaaggaaata tctgcaaagc tgcctaacga atccatccct acactttttt ctgatatcgc
     2521 atcacagtat atactacttc tttgggggaa gtcatatgtc tgaggcagta tccgcagtac
     2581 ttacatgcgc tccctatctt ctggaaggaa tgtgcgtaac ccttgcactg gtagcactgg
     2641 cgctcctgat tgggttcctt tgcggcattg ttctcaccat tggacaggtg tatggtcccc
     2701 gctacattaa gcgtctggtt gacatctacg tctggttctt ccgcggcctg ccgaatattg
     2761 tacttctgtt tctgttctac tttgcgctct tcccgatgac cggttttgat gtgcctgcat
     2821 ttgcagttgc ggttgtagta cttggtctca gaagtgccgc atatcagtcc cagatcttcc
     2881 gcggggccat tgagtcaatt gccgacggtc agatgcttgc cgcacggtcc cttggcatga
     2941 caaagtggca gagtatcaga acggtgatcc ttccccaggc cctccggctc tccctgccgg
     3001 gatggtccaa tgaatacccg gtccttttga cggactccgc cgtcgcctat gttatcggtg
     3061 ttgcagagct tcttaccaga actgcacagg tcatcagtaa aaccggtgag ccgatggttc
     3121 tgtatcttac ctgtgcggtt atcttcatca ttctgaacta cggcggtatg atgcttatcc
     3181 agcgggtgga aaagaaagtc cggatccccg ggttcggcaa caatgaggtg acagaaacat
     3241 gagtgactct ccaattctcc gtgttgaaaa cctctccaaa tcctatggcg atctggaggt
     3301 tctcaagtcg gtatcgtttg atgtctataa aggcgagacc aaggcgttta tcggcccctc
     3361 gggtacggga aagagtacgc ttctccgctg tatcaatcag ctgaccgttc cggacgatgg
     3421 tcacgtgtac ctcaacggcg aggagatcac ccactccgga tccaaaatca atgagtaccg
     3481 ccagaagatg ggcatggtgt ttcagaactt caacctgttt gatcacctga ccgcggtccg
     3541 caacgtggag attgcccttc tcaaggtcaa aaagatgacc aaggaagagg cccgcaagaa
     3601 ggctatgttc gaactggagc gggtgggtat ggcggatcgt gccgactact acccggcaca
     3661 gctttccggc ggtcaggccc agcgtgtctc cattgcccgg gcgctcgcaa tggatccgga
     3721 tgtcatgttg tttgatgagc cgacttcggc acttgatcct gagttgaaac gcgaagtgct
     3781 ggaggtaatg cgaaaacttg ctgcagacgg tatggcgatg ctgattgtca cgcatgagat
     3841 gcactttgcc ac
//
LOCUS       DALTZZ010000123         3210 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_19122,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000123 DALTZZ010000000
VERSION     DALTZZ010000123.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19122"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="O0X12_02310"
     CDS             <1..854
                     /locus_tag="O0X12_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar-specific transcriptional regulator TrmB"
                     /protein_id="HJK53028.1"
                     /translation="DDVEIFHECLSSGCAGFCEFHHRKAGGATLSVTRDGMLLLERSR
                     IGMPVSEPDLTNAAVGLVSVRREGDTIVQTIRSVYGGPAYSLSSMMGQAKGVCGVSIS
                     SADGLVTGEVRTRALVHVTVGIDDTDRKGCGGATFALSQALMKYLTESGDAIGIRHQV
                     AVLSQDIEEKTAGNSCSVLELAVDPETLAGLEAKISRFVEEESVSDNWGVAISQGQHV
                     APDILALASRIRSERVTQEEVLTIAKKHGIATFGGRGVIGAVAAVALRTQPQEVLLDL
                     SYPVMDS"
     gene            891..1436
                     /locus_tag="O0X12_02315"
     CDS             891..1436
                     /locus_tag="O0X12_02315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HJK53029.1"
                     /translation="MNSKITGALLALAALTVVVFAAGCIGTADQTAQVNDTVNVYYTL
                     TLEDGTVRETNVGESPLTFVLGSGQMIKGFDEAVVGMKPGETKTVTLTPDQAYGEYSY
                     DTYADIPVSLAESYNGGPISIGSTFLMYDYSTGSIQVVQGEVMIIDNESNMTRVVINP
                     PLAGKTLTFEITLDSIGEKTA"
     gene            1525..1851
                     /locus_tag="O0X12_02320"
     CDS             1525..1851
                     /locus_tag="O0X12_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53030.1"
                     /translation="MGYTEDKRFQIGWLSVVLMLGIAILVGYSGLGLLAAAGVFLLGV
                     GLIAVALSFAIGKKEPLITGSGALFAIIGAVLMLIYAGAELLLVIGGALAGIALAAIV
                     YVVAKK"
     gene            1879..2415
                     /locus_tag="O0X12_02325"
     CDS             1879..2415
                     /locus_tag="O0X12_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53031.1"
                     /translation="MTDLRNKVEDDRGLLKKIQLGIPGFRGYRQKEDLRIADSLLRMQ
                     IADLLQSDVLGPLEQIRERAGNALELDLMNDIATVISAAKTAESRVRHAEQGYSGISP
                     AYRVSDEQLNTLYEFDLSLVDGIKGLGTLCRTALGSADTGDFGLVKADLRNIRTELSE
                     LLAVFEKRIETMAGLGAF"
     gene            2415..>3210
                     /locus_tag="O0X12_02330"
     CDS             2415..>3210
                     /locus_tag="O0X12_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HJK53032.1"
                     /translation="MALFSKVNKKIGSGSDIEGADSRKGFYWVEDQKGDNVMWRLPRN
                     VMWNDNVLVREDEYGIFFRDGKALAVFDRPDRYALTTQNIPVLKDIAGTVLGNVQIGE
                     FFWVQKREFRDKFGTSQPLAFRDVDFGVVQLRIFGQFSYKVTEPLQLITEFVGTKGLT
                     KSEEIVEWLKGQIVAVLNDTLGELKAKKQMGVLDMPAYLQEIEQLCLAKLTRETEVYG
                     LKIMKFAGLNINMPEEVLEAINKRGAMSALGVNYIQYQSGKAIEGIG"
BASE COUNT          802 a          666 c          888 g          854 t
ORIGIN      
        1 gggatgatgt tgaaattttt catgagtgcc tgagcagcgg atgtgcagga ttctgtgagt
       61 tccatcaccg aaaagcagga ggtgcgaccc tctctgttac acgggatggt atgcttcttt
      121 tggaacgcag cagaataggg atgcctgttt ccgaaccgga tctcaccaat gcggcagtag
      181 gtcttgtttc ggtcaggcgg gagggagata cgatcgttca gacgatccgt tccgtgtatg
      241 gtggtcctgc ttattctttg tcatccatga tggggcaggc aaagggtgtt tgcggagtat
      301 ccatttcttc agctgatggt ctggtcaccg gggaagtgcg gacacgcgcc ctcgtacatg
      361 taacggtggg tattgatgat accgatcgaa aagggtgcgg cggagctacg tttgccctgt
      421 cccaggcact gatgaagtat cttaccgaaa gtggagatgc aataggaatt cggcatcagg
      481 tggctgtttt gtcacaggat attgaggaga aaactgcggg gaactcttgc agtgtccttg
      541 aacttgcagt tgatccggaa actcttgctg ggcttgaggc aaagattagc cggtttgtag
      601 aggaggaaag cgtttctgac aactggggtg ttgcaatttc ccagggtcag catgtcgcac
      661 cggatattct cgcgcttgct tcgcgtatcc gatctgaacg ggtgacgcag gaggaagtgc
      721 tgaccattgc aaaaaagcat ggaattgcga catttggtgg tcgaggagtg attggtgccg
      781 ttgcagctgt tgcacttcgt acccagccgc aggaggtact gttggacctt tcgtatcctg
      841 ttatggatag ctgaacactt ttatacaatt gagtgtaaaa tatattttac atgaactcaa
      901 aaattaccgg ggctttgctg gctcttgcag cccttactgt tgtggtgttt gccgcaggat
      961 gtattggaac tgcagatcag actgcacagg tgaacgatac ggtaaacgtg tattatacgc
     1021 tgacgctgga agacgggacc gtacgtgaga caaatgttgg cgaatctccc cttacttttg
     1081 tattagggtc tggtcaaatg attaagggat ttgatgaggc tgtagttggt atgaaaccag
     1141 gtgaaacaaa gacggtgact ttgactcctg atcaggcgta cggtgaatac agttatgata
     1201 catatgctga cattccggtt tcccttgcag aaagctacaa tggaggtcct atctcaattg
     1261 gttctacctt cctgatgtac gattactcaa ctggttcaat tcaggtagtg cagggagagg
     1321 tcatgatcat cgataatgag agcaatatga cccgtgttgt catcaatcca ccacttgcag
     1381 gaaaaacgct gacctttgag atcacgcttg attccatcgg tgagaagacc gcataatctt
     1441 tttttgaaca gaatgttctg gaaaaagttc tttgcctcat gatgtccaca tctccgaata
     1501 agtacatacg taccacgaat agctatggga tatactgaag ataaacggtt ccagattgga
     1561 tggctgagtg ttgttctgat gctcggcatc gcaattctgg ttggatattc tggcctcggc
     1621 ctgctggctg ccgcgggtgt gttcctgctt ggtgttggac tcatcgcggt tgccctgtcc
     1681 tttgcaatcg gcaaaaaaga accactgatc accggttctg gtgcattgtt tgccatcatc
     1741 ggtgcggttc tgatgctgat ttacgcagga gcagaacttt tactggtcat tggcggagca
     1801 cttgccggta ttgcacttgc tgcaatcgtc tatgttgtag caaagaaata atttgatttt
     1861 tcaaaaataa gaggaaatat gacggattta cgaaacaaag ttgaggatga tcgcggactt
     1921 ttgaaaaaga ttcagctggg aattcccgga ttccgcggat atcggcagaa agaagatctg
     1981 cgtattgcag actcccttct tagaatgcag attgctgatc tgttgcaatc ggacgttctt
     2041 ggtccccttg aacagatccg tgagcgtgcc gggaatgcgc tggaactgga tctgatgaat
     2101 gatattgcaa cagttatctc tgcagcgaaa accgctgagt cacgcgttcg tcatgcagag
     2161 cagggatact caggaatctc tccggcatac cgcgttagtg atgagcagct gaacacactg
     2221 tatgaatttg atctctctct tgttgacgga attaagggac taggtacact gtgccgtaca
     2281 gcactcggtt ctgctgatac cggtgatttt gggttggtaa aagcggatct ccgcaacatc
     2341 agaacagaac tttcagaact tctggcggtg tttgagaaac gtattgagac aatggcagga
     2401 cttggtgcat tctaatggcg ttattctcca aagttaacaa aaagataggc tccggatccg
     2461 acattgaggg agcagactcc cgaaaaggat tctactgggt tgaggatcag aaaggcgaca
     2521 atgtgatgtg gcgccttcca agaaatgtga tgtggaatga caacgtactg gttcgtgagg
     2581 atgagtatgg tatcttcttc cgtgacggaa aggcccttgc cgtctttgat cgtcccgacc
     2641 ggtatgcact gacaacacag aacattcctg tcctaaagga tattgcagga acagttctgg
     2701 gaaacgtcca gataggtgaa tttttctggg tacagaagcg tgagttccgg gacaaattcg
     2761 ggacgagcca gccccttgca ttccgcgatg tggacttcgg tgttgtacag ctgagaattt
     2821 ttggacagtt ctcctacaaa gtaaccgaac ccctccagct gattaccgag ttcgttggaa
     2881 cgaagggtct tacgaaatct gaagagatcg ttgaatggct gaagggacag attgttgcgg
     2941 tgttaaacga cactctcggt gaactgaagg caaagaaaca gatgggtgtt ctggacatgc
     3001 ctgcatacct gcaggagatt gagcagctct gtctcgccaa actgacccgc gaaaccgagg
     3061 tctatggact gaagatcatg aagtttgccg gcctgaacat taacatgcct gaggaggttc
     3121 tggaggcaat caacaagcgc ggtgcaatgt cggcacttgg tgtgaactac atccagtacc
     3181 agtccggtaa ggcaattgag ggtattggaa
//
LOCUS       DALTZZ010000124         3395 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_19252,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000124 DALTZZ010000000
VERSION     DALTZZ010000124.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19252"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /gene="metG"
                     /locus_tag="O0X12_02335"
     CDS             complement(<1..1858)
                     /gene="metG"
                     /locus_tag="O0X12_02335"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HJK53033.1"
                     /translation="MTNKPTVVTCGLPYTNGLCHLGHLRTYIPGDFYVRYLRRLGDDI
                     VFICGSDNHGTPIVVTAEAEHTTPRAVSEYYHAHFDEVFKEMGICFDRFGMTDDPTNH
                     HRTTSIIKTLIEKGYVYEKTIQQSYCPSCQRFLPDRYVEGTCPYCGKHARGDECDQGC
                     GRHLEPGEVLDPVCATCGTKAELREQKHYYFKLSEFRNYLLEYLPTLGGTINARNYAI
                     GWVENELKDWCITRMMDWGVAFPGKDAEGLVTYVWVDAPIGYIAFTEEWAKAAGTSWQ
                     KYWCDGDRVHFIGSDIIYHHCVFWPAMLHGAGYQPPSAVVASGMVTIDGQKFSKSRGY
                     VVWTKEDYLDKGLPADYLRYYLLSYTSHTRELDFSWKEFQARINNELVNTFGNFANRS
                     MTLVWTKLGEVPDVPAQQEIFDEISKTLCSVEEAVRSFEFKAAVDGILLLAGYGNSYI
                     SNAAPWKLIKEDPAAASQVLKNCLQIVKACALLIQPVMPESAQKLWKMLGYTDKVEDH
                     PIGDALVPFENKTLGEVKPLFARIEDKQREELEATLAKRAVEAQNKAAGKNTMEAEID
                     PFLEDIVTIDDVTKLDLRVGLVVKAEKVPKTEKLLRLQVDVGKEVRQIISSIA"
     gene            2121..2912
                     /locus_tag="O0X12_02340"
     CDS             2121..2912
                     /locus_tag="O0X12_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53034.1"
                     /translation="MRELSFRVRSLGSAKPAVPAPEVLGDWIRKHTGTTADLITWEVE
                     TTVLSQGSEVDFPAAGGMWYCDRICAGFPNVSDHQISGEFDFVSADLVSDCGIVSKMR
                     KGCWWAVPSPQALHLTDGYFRDEEYASEEVADALTRVCRVMRDAGATGHILLYDDAPD
                     AIDLEHFSGKRFLRYVPDTFLEDVLEVQRDLILSADAVPHLADLADSYDIRHVYIVEP
                     TVDVLAEVCRLFDCEDVFCAGIAPESDQNAYWQSLAELRIQVRDV"
     gene            complement(2924..>3395)
                     /gene="rpiA"
                     /locus_tag="O0X12_02345"
     CDS             complement(2924..>3395)
                     /gene="rpiA"
                     /locus_tag="O0X12_02345"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HJK53035.1"
                     /translation="YGIPLTTLTLHPKLDIAIDGADQVDPKKQLIKGRGAAHVREKIV
                     ADAAKQFVVVIDSGKEVTGLDAAVPIEILPFAYGSVAKKLQELGGEPVMRNGVKKDGP
                     VISDNGNYVIDCSFGAITDPSALEATINNIPGVVGNGIFASMTEKTIVIVGGRS"
BASE COUNT          778 a          820 c          878 g          919 t
ORIGIN      
        1 gcgcaatact ggagatgatc tgacggacct cttttccgac atccacctgt aatcgcagaa
       61 gcttctcggt cttagggacc ttctctgctt ttaccaccag acccacgcga aggtcaagct
      121 ttgttacgtc gtcaatcgtt acaatatctt ctaagaacgg atcaatttct gcttccattg
      181 tattctttcc tgcagcctta ttctgtgctt caacagcacg ttttgcaagg gttgcctcaa
      241 gttcttcacg ctgtttgtcc tcaattcttg caaacagcgg ctttacctca ccaagtgttt
      301 tgttctcaaa cggaaccagt gcatcaccaa tcggatgatc ttcaacctta tccgtgtagc
      361 cgagcatctt ccagagtttc tgggcagatt caggcatcac cggctgtatc aggagtgcac
      421 aggctttgac gatctgcaga cagttcttca gtacctgtga tgcggccgcg ggatcttctt
      481 taatcagttt ccagggtgca gcgtttgaga tgtaactgtt accatatccc gctaacagca
      541 ggatgccgtc aaccgctgct ttgaactcaa atgaacgtac agcctcctcg acggaacaaa
      601 gggtctttga aatctcatca aagatctcct gttgggcggg tacatccggt acttcaccaa
      661 gcttcgtcca gacaagtgtc atactgcggt ttgcaaagtt accgaaggtg tttacgagtt
      721 cgttgttgat gcgtgcctgg aactccttcc atgagaagtc aagttcgcgg gtgtggctgg
      781 tgtaagagag cagataatac cgcagataat ctgccggaag tcctttgtca aggtaatctt
      841 ctttggtcca cacaacgtat ccacgggact tggagaactt ctggccgtca atcgtgacca
      901 taccgcttgc aacaacagct gatggcggct gatagcctgc gccgtgcagc attgcaggcc
      961 agaacacaca gtgatggtag atgatgtcag aaccaatgaa gtgtacacga tccccgtcac
     1021 accagtactt ttgccagctg gtacctgcgg ctttcgccca ttcctcggta aaagcaatgt
     1081 agccgatcgg tgcatcaacc cacacgtagg tgactagacc ctctgcatct tttcctggga
     1141 acgcaactcc ccagtccatc atccgggtaa tgcaccagtc cttcagttca ttctcgaccc
     1201 acccaatggc atagttccgg gcattgatgg ttccgccaag tgtcggaaga tactcaagca
     1261 gatagttgcg gaactcactc agtttgaagt agtagtgttt ctgttcgcga agttcggctt
     1321 ttgttccgca ggtggcgcaa acgggatcaa gaacctctcc aggttcaaga tgtctgccgc
     1381 agccttgatc gcattcgtca ccgcgggcat gctttccgca gtagggacat gttccttcca
     1441 cataccggtc ggggaggaac cgctgacaac ttgggcagta actctgctgg atagtctttt
     1501 catagacgta acccttttcg atcagggttt tgatgattga ggtggtccgg tggtgatttg
     1561 ttggatcatc ggtcataccg aaccgatcga aacagattcc catctctttg aaaacttcat
     1621 cgaagtgggc atgataatac tcggagacgg cacgcggagt tgtgtgctcg gcttctgctg
     1681 tgacaacgat cggagttccg tgattgtcgg aaccgcagat aaacacgatg tcatcgccaa
     1741 gcctgcgcag gtagcggaca tagaagtcgc cgggaatgta ggtcctcaga tggccaagat
     1801 ggcacagacc attggtataa ggaagaccac aggtgacgac tgtcggctta ttggtcatag
     1861 tggttattat ttttgcatgg atgaggtatt agggtttgta agaggtagtt gtgcagatag
     1921 taacgagcgt tggctccgtc tctaaaccca tccagatttc ttttacctcg tcgtctgaga
     1981 tgtactggtt agctgggtgt tttctccatt cgctagggat acagacgatt ggaatccata
     2041 ggatcttttt tggttcctcg gatttggagg ataacagcat tccatagttt caatacgcat
     2101 ctgctgaata atgaatactc atgcgtgaac tgtcgtttcg ggtaaggagt ctcggatcgg
     2161 caaaacctgc agttcctgcg cccgaggtgc ttggagactg gatacgaaag cataccggaa
     2221 ccactgcgga tctgattacc tgggaagtag aaaccactgt cctgtcgcag ggatctgagg
     2281 tggattttcc tgcagctgga gggatgtggt actgcgaccg gatctgtgca ggatttccga
     2341 atgtttccga tcaccagatc tccggagagt ttgattttgt atccgctgat ctcgtatcag
     2401 actgcgggat tgtttcaaag atgcggaaag gatgctggtg ggcagttccg tctccacagg
     2461 ctctgcatct tacagacggt tatttcaggg acgaagagta cgcatccgag gaggttgcgg
     2521 atgcgctaac gagggtctgt cgggttatgc gggatgcagg tgcaacaggg catatactgt
     2581 tgtatgatga cgctcctgat gcaattgatc tggaacactt ctctggaaaa cgatttctcc
     2641 ggtatgtgcc ggatacattt cttgaggacg ttttagaggt gcagcgggat ctgattcttt
     2701 ccgcggacgc ggtaccgcat cttgcagacc ttgctgacag ttatgatatc cggcatgtgt
     2761 atattgtaga acctactgtg gatgtccttg cagaggtatg tcgtctgttt gactgcgagg
     2821 atgtgttttg tgcagggatt gctccagaga gcgatcagaa cgcctactgg cagagccttg
     2881 ctgagctgcg gatacaggta cgggatgttt gaaaaaagag gagttaggat cttccgccaa
     2941 caatgacaat tgtcttctct gtcattgatg caaagatgcc atttcctaca acacctggga
     3001 tgttgttgat tgtcgcttca agggcagaag gatcagtaat tgcaccgaag gaacaatcga
     3061 tcacatagtt gccgttgtcg gagattaccg ggccgtcttt tttcacaccg ttccgcataa
     3121 ccggctcacc tcccagctcc tgaagttttt ttgccacact gccgtaggca aagggaagga
     3181 tctctatggg tacagctgca tcaaggcctg taacctcttt tccggaatcg atcacaacaa
     3241 caaactgctt tgctgcatca gccacaattt tttcccggac atgcgctgcc ccgcggcctt
     3301 tgatcagctg ttttttggga tcaacctgat ctgcaccgtc gattgcgatg tcaagctttg
     3361 gatgcagcgt cagcgtggta agcggaatac catac
//
LOCUS       DALTZZ010000125         1745 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_19284,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000125 DALTZZ010000000
VERSION     DALTZZ010000125.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19284"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="O0X12_02350"
     CDS             complement(<1..784)
                     /locus_tag="O0X12_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase subunit beta"
                     /protein_id="HJK53036.1"
                     /translation="MNPIRLRDFVMTGDGCLYAVSGYENDERAECVLRYVPTPDGDRT
                     APWGTRYKKYDFADAFAWVKEHKPEYLDLVHRVPLTDIVRVLKPEEQAASIAARNPRV
                     ARLFSHFDLPPMTWGCTGSLLAGLENDNSDIDMVVYGSAWFTARQQLMDAVAKGKIPA
                     MSEEMWQKVYRKRVPDISFDDFVLHESRKFNRGEFEGTYFDLLYSRGYDNLHSVPPIT
                     LGEKTGKMTIEATVTDASLAFDSPGIYRVDHEEIDLVLSFTHT"
     gene            complement(798..882)
                     /locus_tag="O0X12_02355"
     tRNA            complement(798..882)
                     /locus_tag="O0X12_02355"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(844..846),aa:Leu,seq:taa)
     gene            982..1054
                     /locus_tag="O0X12_02360"
     tRNA            982..1054
                     /locus_tag="O0X12_02360"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1016..1018,aa:Gln,seq:ttg)
     gene            1120..>1745
                     /locus_tag="O0X12_02365"
     CDS             1120..>1745
                     /locus_tag="O0X12_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HJK53037.1"
                     /translation="MKVLIAEYTASRDPTLAPEGKAMVSVLQESFERLGHTVVLPESG
                     DFDAEIARLAPQCDYGLVIAPDEMLSKFTHTLELATHNIGSDSTSIAVCANKRLTGKI
                     LSGIGIAVPQEVGADYTGKRVIKPVKGAGSVGVRIAKEGEVPGDGEMAVEYLEGEHYS
                     VSIVGSRVVGEACGYYSGLPPVFLTINRQYSSADENGVFTYRGGETPV"
BASE COUNT          393 a          458 c          513 g          381 t
ORIGIN      
        1 aggtgtgggt gaaggagagg acgaggtcga tctcttcgtg atccacgcgg tagatgccgg
       61 ggctgtcaaa ggcaagggag gcatcggtga cggttgcttc gatggtcatc ttaccggttt
      121 tttctcccag ggtaatcggc ggtacggagt gaaggttgtc gtaaccgcgg gagtagagaa
      181 ggtcaaagta ggttccttca aactcgccgc ggttgaactt ccgtgattcg tgcaggacga
      241 agtcgtcaaa ggagatgtca ggcacgcgtt tgcggtagac tttctgccac atctcctcgc
      301 tcattgcggg gatttttcct ttggcgaccg catccatgag ctgctggcgc gcggtaaacc
      361 aggcacttcc gtacaccacc atgtcgatgt cggagttgtc gttttccaga ccggcaagga
      421 gagagcccgt acagccccag gtcatcggcg gcaggtcaaa gtgggagaac agccgcgcaa
      481 cacgcgggtt tctggctgcg atggatgcgg cctgctcctc gggtttgagg acacgtacga
      541 tgtctgtgag gggcacacgg tgtacgaggt cgaggtactc gggtttgtgc tcctttaccc
      601 aggcgaatgc gtcggcaaag tcgtattttt tgtagcgcgt gccccacggc gcggttctgt
      661 cgccgtccgg ggttgggaca taccggagaa cacactctgc acgttcgtcg ttttcatacc
      721 cggatacggc atacagacat ccgtcgccgg tcattacaaa atcgcgtaaa cgaatcggat
      781 tcatagaaaa gacctgatgc gagggaaggg attcgaaccc ttgaatcgct accgaactgg
      841 atcttaagtc cagcgccgtt ggcctggctt ggctaccctc gcaaactacc ttattagata
      901 gcacttccaa cataaaaaaa cgaccgcaga attggcaacc gatatatgcc aacagaacag
      961 aatgatataa gaacagcccg gactctcgta gtgtagtggt caatcattcc ggcctttgga
     1021 gccggagacg gcggttcgaa cccgcccgag agtatgaaat ctctttttgt tcccctatcg
     1081 gcagcctgat gtaatgccgt cttcaaatac attagtcaca tgaaggtcct catcgctgaa
     1141 tatacggcaa gccgtgatcc gacccttgcc ccggaaggaa aggcaatggt ctctgtactg
     1201 caggaaagct tcgagcgttt aggccatacc gtggttctgc ccgagtcagg tgatttcgat
     1261 gcagagattg cgcggcttgc gccgcagtgc gactacgggc tcgtcattgc cccggatgag
     1321 atgctctcca agttcaccca caccctggag cttgcaaccc acaatattgg ttctgacagc
     1381 accagcatcg ccgtctgtgc aaacaaacgc ctgaccggaa agattctctc cggaatcggt
     1441 attgcagtcc cgcaggaggt tggagctgac tacaccggaa aacgcgtgat aaaaccggtc
     1501 aaaggagcag gttccgtcgg agtccgtatc gcaaaagagg gagaggtccc cggcgacggc
     1561 gagatggctg ttgagtatct cgaaggagaa cactacagcg tcagtatcgt gggcagccgt
     1621 gtggttggcg aggcctgcgg atactacagc gggcttccgc cggtgtttct gaccattaac
     1681 cgccagtatt catccgccga tgagaacggg gtcttcacct accgcggagg agagactccg
     1741 gtgca
//
LOCUS       DALTZZ010000126         6705 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_19616,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000126 DALTZZ010000000
VERSION     DALTZZ010000126.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19616"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="O0X12_02370"
     CDS             complement(<1..200)
                     /locus_tag="O0X12_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833886.1"
                     /note="the function of this ribosomal subunit is unknown;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HJK53038.1"
                     /translation="MVDFKVVLSDPKTGLSYKIDATGAAAGALLGKKIGTEVDGAPFG
                     LNGYKITITGGSDKTGTPARPDL"
     gene            complement(272..2044)
                     /gene="infB"
                     /locus_tag="O0X12_02375"
     CDS             complement(272..2044)
                     /gene="infB"
                     /locus_tag="O0X12_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HJK53039.1"
                     /translation="MAAEHIRTPIVCVLGHVDHGKTSLLDRIRGSRVVAGEAGAITQH
                     IGATMIPIDSIQKMSGELGKLKTTVPGLLFIDTPGHHAFTTLRARGGALADIAILVVD
                     VTEGFKQQTIEALQILRNCKTPFVIAATKIDRIPGWRPMENASFLKSYKNQNERVQNE
                     CENRIYELVGKLSEMGFNSERFDRVSDFQRNLVIVPVSSMTGEGIGDLLMVMIGLAQR
                     FLTEGLKTTVKGPGVGTVLEVKEEKGLGTTLDVILYDGIINVGDEIAIAGTEGAIATK
                     VRALLQPRPMKEILIEDRFQRVKSVTAAAGVKITAPNLEDIIAGSPVRVIRGDRDEML
                     AKIEEEMREINVKLSEIGITVRADTIGALEALSKELEEKNIPIMRAEVGPVSRRDLIE
                     ISVMKDELYKTVLCFNVPLLPDAEAMVRDEEVDVKIFSNRVIYKLIDDYIDWRDELTR
                     AREAKQFETVVLPAKFSILPDCVFRQSGPAVVGVRVLGGILRPHVGVATRDGKVVGEI
                     KQIKLNKENIQEAKEGLEVAVSIEGVTIGRQIDVGETLYVAVPERHVKVLETEMLSHL
                     NTGTQEALEEYTNIFRKTQPFWGK"
     gene            2244..2316
                     /locus_tag="O0X12_02380"
     tRNA            2244..2316
                     /locus_tag="O0X12_02380"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2278..2280,aa:His,seq:gtg)
     gene            complement(2336..2695)
                     /locus_tag="O0X12_02385"
     CDS             complement(2336..2695)
                     /locus_tag="O0X12_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJK53040.1"
                     /translation="MDEFCTVNLTVRYLTKKWTLLIILELFKGDNYTRRFSELKERLP
                     DITAKVLSERLQELEAEGIVSKTIDATVVPIRSEYTLTESGIELMEVIRGIKYWALKW
                     KIDNIPCGSQECKLCKL"
     gene            complement(2759..4645)
                     /locus_tag="O0X12_02390"
     CDS             complement(2759..4645)
                     /locus_tag="O0X12_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK53041.1"
                     /translation="MHPGQFAGGRGPSDAGKTIKRLFGYLTGMDRVRFAVVLICIVIS
                     ALAGVVSSLFIEVLIDNYITPLIGVENPVFTGLLSAILFMAVIYTAGVAATFVYTRLM
                     VVIAQGMLKTIRDRMFSHMQKLPVSYFDTHTFGDVMSRYTNDTDTLRQMLSQSVPQMF
                     SSVVTIVVVLAAMIYTSGYLTVLVLVMVGVLFFVTKYIGGKSATYFIQQQESLGEVNG
                     YIEELMGGQKVVKVFCHEDAAKAEFDSMNDKLCGHATSANTFANIFMPVVANIGNIQY
                     VLVGIFGGALALLGVGGLTLGGIAAFLLLSRSFMMPISQVSQQISAVVMALAGAERIF
                     QLIDEPVEADTGTIRLVNVKREGDSLVETTSETTTWAWKEETDEGTVYTELTGDVQFV
                     QVSFGYTPEKEVLHDISLYAKPGQKVAFVGATGAGKTTITNLINRFYDISAGEILYDG
                     IDVTRMKKSDLRWSLGMVLQDTNLFTGTVMENIRYGKLNATDEEVYAAAKLANADEFI
                     RMLPEGYATMLEGDGGSLSQGQRQLLSIARAAVANPPVMILDEATSSIDTRTEAIVQA
                     GMDSLMKGRTVFVIAHRLSTIHNADVILVLENGKIIERGNHETLIAQKGKYYQLYTGA
                     FELE"
     gene            complement(4669..6405)
                     /locus_tag="O0X12_02395"
     CDS             complement(4669..6405)
                     /locus_tag="O0X12_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK53042.1"
                     /translation="MNRIVERLSGSIREYRRDSILAPVFVALEVVMEVLIPLVLADLI
                     DDGITAGNMDIIIKLGLVLLLFAIMSLVFGVFAGKYAASASAGFAKNLRHDLFYAVQN
                     FSFANIDKYSTASLITRMTTDVTNVQNAYQMIVRIAIRCPLMLIFAFLMVVGIHAQLA
                     LIFLVLLPILGIGMYLIITRAQPLFELVFKTYDSLNKVVRENLRGIRVVKSYVREDYE
                     IKKFRNVSGSIYGYFSRAEKILAFTSPLMQAVVYASILLISWFGAQFIISGSLTRGDL
                     ISLISYSMQILMSLMMLAMVFVMITMAQASSRRIAEVLEEEVDLTNPSDPVFTVADGS
                     VEFRDVGFQYTKDAEQEILKDINLKIRSGETVGILGGTGSAKSTLVQLIPRLYDTTKG
                     EVLVGGVNVREYNLTTLRDAVAMVLQKNVLFSGTIRDNLKWGNPDATDEEIARACRLA
                     KADEFIETLPEGFDTKVEQGGANFSGGQKQRLCIARALLKKPKILILDDSTSAVDTRT
                     DALIRQAFRTEIPDTTKIIIAQRIASVQDADNILVMDAGRICAMGTHEELLQNNAIYQ
                     EMYRSQMKGDDA"
     gene            complement(6402..>6705)
                     /locus_tag="O0X12_02400"
     CDS             complement(6402..>6705)
                     /locus_tag="O0X12_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HJK53043.1"
                     /translation="PERDIFQKDIESILSIRRSTATGILQLMEKNGLIIREPVPYDAR
                     LKKLVPTQKALAVNRRLEEEFKRINAQLIHGLTKEELDIFCSVAEKMQANLNGGES"
BASE COUNT         1551 a         1766 c         1534 g         1854 t
ORIGIN      
        1 aggtccgggc gtgccggagt tccggttttg tccgatccac cggtgattgt aatcttgtaa
       61 ccattcagac caaacggagc gccgtcaacc tcggtgccga tcttctttcc cagaagtgca
      121 cccgcagctg cgcccgtggc atctattttg tacgaaagac cagttttcgg gtctgacagt
      181 acgaccttaa agtctaccat tgtattagca catctcctcg atatcaatag gatgccgaag
      241 tacccatata gttagtctct tcccatttat actacttccc ccagaagggt tgggtcttgc
      301 ggaagatgtt ggtgtactct tccagtgcct cctgcgttcc agtatttaaa tgagacaaca
      361 tttctgtttc cagaactttc acatgccgct ctggcacggc aacgtacaat gtctcgccaa
      421 cgtcgatttg ccgcccgatg gtgacgcctt caattgaaac agcaacctcg agaccttcct
      481 ttgcttcctg aatattttcc ttgttgagtt tgatctgttt gatctctcct actacttttc
      541 cgtcgcgcgt cgcaacgccg acgtgaggtc ggagaattcc tcccagaacc cgtacaccca
      601 ctaccgcggg tccgctctga cggaacacgc agtcgggcag gattgaaaac ttcgccggaa
      661 gaaccactgt ttcaaactgt tttgcttccc gtgcgcgggt aagctcgtcg cgccagtcga
      721 tatagtcgtc gatgagtttg tagattacgc ggttggagaa gatctttaca tcaacttcct
      781 catcgcggac cattgcctcc gcatcgggca gaagcgggac attgaaacag agcaccgtct
      841 tgtagagttc gtccttcatc acactgatct cgatcaaatc acggcggctg accggaccta
      901 cctctgcacg cataatcggg atgttcttct cttccaattc tttggagagg gcttcaagcg
      961 cgccgatggt gtctgcgcgg accgtgatgc cgatctcaga aagtttcacg ttgatctcac
     1021 gcatctcctc ttcaatcttg gcaagcattt catcacggtc tccgcggatt acacggactg
     1081 gtgatcccgc aatgatgtct tccaggtttg gcgcggtaat cttcacacct gcagctgccg
     1141 tgaccgactt tacacgctgg aaccgatcct cgatcagaat ctctttcatc ggccgaggct
     1201 gcaacagtgc ccgaactttt gtggcaattg ctccctcggt tcctgcaatt gcaatctcat
     1261 caccgacgtt gataataccg tcatagagga tcacgtcaag tgttgttcca agacctttct
     1321 cctctttaac ctccaatacg gtacccacac cgggcccttt tactgttgtc ttcagccctt
     1381 cggttaagaa ccgctgcgca agaccgatca tcaccatcag gagatcgccg ataccttccc
     1441 cggtcatgct gctgaccgga acaatcacca ggttgcgctg gaagtcactg acacgatcaa
     1501 accgctcgga gttgaatccc atctcggaaa gttttccgac taactcatag atccggttct
     1561 cacattcgtt ttgtacgcgt tcgttctggt ttttatagga cttcagaaac gatgcattct
     1621 ccattgggcg ccagccggga atacggtcaa tctttgttgc cgcaattaca aatggtgtct
     1681 tgcagtttct aagtatctgg agtgcttcaa tggtctgctg tttgaatcct tcagtgacat
     1741 ccaccaccag aattgcaatg tcggcaagtg caccgccacg ggcgcggagt gttgtgaagg
     1801 catgatgtcc gggtgtgtcg ataaagagaa gacccgggac tgttgttttc agttttccca
     1861 gttcaccgct catcttctga atcgaatcga tcgggatcat cgttgcgccg atgtgctggg
     1921 taatcgctcc agcctccccg gcaacaaccc gcgatccccg gatccgatcc aggagcgatg
     1981 ttttcccatg gtccacgtgc cccaacacgc agacaatggg agtacggata tgttctgctg
     2041 ccatttctct ttctctcctc tctgatttta aaaatcagag taaaaactac gtaagtattg
     2101 gcagtggtca ttaataaacg taaatggcgg ttggctcgcc atctaaagga tatgttaata
     2161 tttgggaaca ccctatgtat tagagtgttt catacaatgg tatgaagcaa gatcgtaact
     2221 ggtccagatt tgctggttct caggctaagg tagggtagct ggacatccta taggactgtg
     2281 gatcctttga cccgggttcg agtcccggcc ttggcccttt tgtattttat ttttattaca
     2341 gtttacagag tttgcattcc tgactgccac agggaatgtt gtcaatcttc cattttaatg
     2401 cccagtattt gatgccccgg ataacttcca tcagttcaat gccgctctca gtcagggtgt
     2461 actcggagcg aattggaaca acggttgcat cgatcgtttt tgatacaatt ccttctgctt
     2521 ctagttcctg aagccgttcg gagagaactt ttgccgtgat atccggcagc ctctctttca
     2581 gttccgagaa tctgcgggta tagttgtctc ctttgaaaag ttcgagaata attaatagtg
     2641 tccatttttt tgtcaggtac cggactgtca ggtttacggt acagaactca tccataaaaa
     2701 tagatgggga gtgtactcct atctactttc gtatcttttg gatactacgt atgtattgtc
     2761 actcaagctc aaatgcgccc gtgtacagct ggtagtactt tcctttctgt gcaatcaggg
     2821 tctcatggtt gccgcgctca attattttgc cgttttccag aacgagaatc acatccgcat
     2881 tgtgaatcgt cgacagccgg tgggcaatca cgaacactgt ccggcctttc attagtgagt
     2941 ccatccctgc ctgcacgata gcctctgttc tggtgtcaat actggaggtc gcctcatcca
     3001 gaatcattac cggcgggttt gccaccgctg cacgtgcaat ggagagtaac tggcgttgtc
     3061 cctgtgaaag actgccgcca tcaccctcca gcattgttgc atatccttcc ggaagcatgc
     3121 ggataaactc atccgcgttt gcaagctttg ctgctgcata tacttcctca tcggttgcgt
     3181 tcagtttacc gtaccggatg ttttccatca cagttcccgt gaagaggttt gtatcttgaa
     3241 gtaccattcc aagtgaccac cgcagatcgc tttttttcat tctggtcaca tcgatgccgt
     3301 catagagaat ctctcccgca gaaatatcgt aaaaccggtt gatcaggttt gtgatagttg
     3361 tctttcccgc accggttgct ccgacaaacg caactttctg tcctggtttt gcatacaggg
     3421 aaatgtcgtg cagaacttcc ttctccgggg tgtagccaaa actcacctgc acaaactgta
     3481 cgtcgccggt caattccgtg tagaccgtac cttcatcagt ctcctctttc catgcccagg
     3541 ttgttgtttc actcgtcgtt tccacaaggc tgtctccttc acgtttgaca tttaccagcc
     3601 gaatggttcc ggtgtctgcc tcaaccggct cgtcgattaa ctggaagata cgctctgcac
     3661 cggcaagagc cattaccacc gcgctgatct gctgtgagac ctggctaatc ggcatcatga
     3721 aacttctgct cagcagcagg aatgctgcaa ttcctccaag cgtcagacca cctaccccga
     3781 ggagtgcaag tgcccctcca aagattccca ccagtacata ctggatgttg ccgatgtttg
     3841 ccacaaccgg catgaagatg ttggcaaagg tatttgctga tgttgcatgg ccgcagagtt
     3901 tgtcgttcat cgaatcaaac tcagcctttg ctgcatcttc atgacagaac accttcacta
     3961 ctttctgacc tcccatgagc tcttcgatat agccgttcac ttcaccgaga gactcctgct
     4021 gctggataaa gtaggtcgca ctttttccgc cgatgtattt ggttacaaag aacaacacac
     4081 ctaccatcac gagaaccagg actgttagat atccgctggt gtatatcatc gcggctaaaa
     4141 ccactacgat tgtcactacg gatgagaaca tctgcggaac actctgtgaa agcatctgcc
     4201 ggagagtatc tgtatcattg gtgtaacggc tcattacatc tccgaaggta tgcgtgtcaa
     4261 agtagcttac cggcagcttc tgcatatgcg aaaacatccg gtcacggatt gttttcagca
     4321 tcccctgagc aataaccacc atcagccgcg tatagacaaa cgttgctgca actcctgcgg
     4381 tatagattac ggccatgaag agaattgccg agaggagtcc ggtgaatacc ggattttcca
     4441 ccccaataag cggcgtgatg taattatcga taagtacctc aataaacagg gaactgacaa
     4501 cacctgcaag ggcactgata acaatacaga tcagaacaac agcgaaccgt actctgtcca
     4561 tgcctgtcag gtacccaaac agcctcttga tcgtctttcc cgcatcactg ggtcccctgc
     4621 ctccggcgaa ctgtccagga tgcatacgtc ttcctcccat cggcggcatt atgcatcatc
     4681 tcctttcatc tgcgaccggt acatctcttg gtatatcgca ttgttttgta acagttcctc
     4741 atgtgtcccc atggcacaga tacggccggc atccataacc aggatattgt ctgcatcctg
     4801 taccgacgca attctttgag cgatgatgat ctttgttgtg tcgggaattt ctgttctgaa
     4861 tgcctgccgg atcaaagcgt ctgttcttgt atcgactgca cttgttgagt catccagaat
     4921 gagaattttt ggttttttca gtagtgcccg tgcaatacaa agcctctgtt tctggcctcc
     4981 ggagaaattt gcccctccct gttccacctt tgtgtcgaac ccttccggta gtgtttcaat
     5041 gaactcgtcc gcctttgcaa gtctgcatgc acgggcaatt tcttcatcgg tcgcgtcggg
     5101 atttccccat ttgagattat cacgaatggt tccggagaac agtacattct tttgtaaaac
     5161 cattgcaacc gcatcccgca gggtggtgag attatactcc cgtacgttta ctcctccgac
     5221 gagaacctcg ccttttgttg tatcatataa ccggggaatc agctgtacca gggtcgactt
     5281 tgcactcccg gtacctccaa gaattccaac cgtttctcca gatcggattt tcagattaat
     5341 gtctttgagg atctcctgtt cggcatcttt tgtgtactgg aatcctacat cacggaactc
     5401 cacgctcccg tctgcaactg tgaaaaccgg atctgatggg ttggttaagt ccacctcttc
     5461 ttcaagtacc tcggcaatcc tccgggatga cgcctgtgcc attgtgatca tgacaaagac
     5521 catggcaagc atcatgagac tcatcagaat ctgcatagag tatgagataa gacttatcag
     5581 gtcgccccgt gtcaggcttc cagaaatgat gaactgcgcc ccaaaccagg atatgagaag
     5641 aatacttgca tagacaactg cctgcatcag aggactcgta aatgcaagga tcttttctgc
     5701 acgcgagaag tacccgtaga tggatcctga gacgttgcgg aactttttga tctcataatc
     5761 ttcccgtaca taggacttta ccacacggat cccgcgaagg ttttcccgca ccactttgtt
     5821 gagcgaatca taggtcttaa acaccagttc aaacagcggc tgtgcacgtg tgatgatcag
     5881 atacatcccg ataccaagga ttggcagaag tacaaggaag atcagtgcca gctgtgcgtg
     5941 aattccaacc accatcagga acgcaaagat cagcatcagc ggacagcgga tggctatcct
     6001 aacgatcatc tgataggcat tctggacgtt ggtgacatca gttgtcatac gggtgatgag
     6061 acttgccgtt gagtacttgt cgatgtttgc aaacgaaaag ttctgaactg cgtagaagag
     6121 atcatgccgg agattttttg caaatcctgc agacgcggat gctgcgtact ttcccgcaaa
     6181 gacaccgaag acgagtgaca ttatcgcaaa cagcagcaga acaagtccga gctttatgat
     6241 gatgtccatg ttgccagcgg tgattccgtc atcaatgaga tccgcgagaa ccagaggtat
     6301 cagaacctcc ataacgacct ctagtgcgac aaataccggg gcaagaatac tgtcacgtcg
     6361 gtactcgcgg atagatccgg atagtcgttc aacgattcgg ttcacgattc tcctccattg
     6421 agatttgcct gcatcttttc ggcaaccgaa cagaagatat ccagttcttc cttggtaaga
     6481 ccgtgtatca gttgtgcgtt aatccgtttg aattcttctt cgaggcggcg gtttactgcc
     6541 agtgctttct gtgttggaac cagtttcttg agccttgcat catatggtac tggttcacga
     6601 atgatgaggc cgtttttttc cataagttgg agtattcccg ttgctgttga tcgccggatg
     6661 gagagaatgg attcaatgtc tttctgaaat atatcacgtt cgggg
//
LOCUS       DALTZZ010000127         5966 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_19650,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000127 DALTZZ010000000
VERSION     DALTZZ010000127.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19650"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="O0X12_02405"
     CDS             <1..418
                     /locus_tag="O0X12_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJK53044.1"
                     /translation="SRTLMQLLAELDGFDNRGDVKVVGATNRPDILDRALLRPGRFDR
                     IIEFPLPDEEGRRTILAIHTRKMNLRKTVSLAEIAKETEGMNGSELMAVCVEAGMNAI
                     RSRRTTITQDDFDKALAAVKTGRSGVVVQAPDAMYS"
     gene            569..3178
                     /gene="mutS"
                     /locus_tag="O0X12_02410"
     CDS             569..3178
                     /gene="mutS"
                     /locus_tag="O0X12_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HJK53045.1"
                     /translation="MVPQKKLTPAMEQVRMFKEQYPDCILFMRMGDFYETFWEDAEIC
                     ARELDIVQTSRSKDPDGNPIPLAGIPYHALDLYLPRMIRKGYKIAICEQIEDPKTAKG
                     CVKRDVIRVVTPGTAIDSGIVDTSAAHYLMAVAADTKGRAGLAFLDITTGEFFVEDIP
                     LEENNASLATEIERYTPAEILVPQQIPANLLTFLAGCGRVITPGRAGLFDDGEQALCT
                     AFGVASLDGYDCADSPLCISAAAAALRYAKETQKTALPHISGFFRRHSGDAMVLDAIT
                     LRNLEILSPLRGDRNDTTLFGFLNRTKTPMGSRTLRSTVTVPLLDAATINRRLDAVEF
                     FTNRPVLCKTLDGVLAKVADIERIAGRIAYGNASPRDLLSLAASLSILPEIEKEIGKT
                     SGLLAEQLARIPDFSGVAGLITSAIIEDPPAVYRNGGVIRAGYNADLDELRTVVATGR
                     DWIAELQQSERDRTGIKSLKIAYNNVFGYYIEVTKANISKVPPEYERKQTTVNGERFT
                     LPALREREALMAQADDRILALEVALYEALLATLRDHVPELKEAAEGIGTIDMLVSFAE
                     LASANRYVRPEFSAGADLLIRDGRHPIVEDSVPGGYVPNDTEMNASGDQILILTGANM
                     AGKSTYMRSVALICIMAQIGSFVPARYARIGMVDRIFTRVGASDDLAGGQSTFMVEML
                     ELANILNNATDRSLILLDEIGRGTSTVDGYAIARAVLEFLHGKGASGPRTLFATHFHQ
                     LIGMEQEFRRMKNYHFAVKEDQHDITFLRKLIPGATDRSYGIHVARIAGVPKKVLTRA
                     EAILNQALREDAGSGGRRYYTQMLLVDTTAEPLTPEVSAVEERVREANINEMTPMQAL
                     MFINELKSMLERK"
     gene            3178..4992
                     /gene="mutL"
                     /locus_tag="O0X12_02415"
     CDS             3178..4992
                     /gene="mutL"
                     /locus_tag="O0X12_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HJK53046.1"
                     /translation="MGVITVLDEETISHIAAGEVVERAASVVKELVENAVDAGAKRIK
                     IDLAADKTAVTRIAVTDDGCGMDDEDALLAFRQHATSKISRPEDLASIGTLGFRGEAM
                     ASIAAVSQVTLTTKKQGAKTPEATRVVVHGGELVEHTATGAPEGTTVIVEGLFYNTPA
                     RRKFQKTVATELAHIYDMVERMALAHRDISFVLSYQGKERFRTFGNGSFEDVIAAVFG
                     QSFAKELVPVKGTYGIVTVSGFVTRPGSEMKSTPSRFYLSINRRQISSRPLQWAVREG
                     YGTLLPKGMYPAAFLDLEINPSDVDVNVHPTKKEVRLSREREVQTGVQDAVYCALHET
                     RVFAAADQQSHTEPRKDSGKNVVSMLPFSVLGESQSPYERRITPQKKETAAPKIQAAA
                     LRQTDRQLRRTERFDPPETAEFVPEVLGQIADTYILARNETGDLVVIDQHAAHERVMY
                     DLLLARQEAGLSGQELLVPVQLKLTKKEAAAVPDLLPVLADAGYTLEPFGKDVWMVRT
                     VPVVSASLGDPAVIHEIIAQALDKTSSGGEESVLDRVLKTAACRAVVKGATPMTTEQM
                     QRLLRQLMATRSPYTCPHGRPTTIVLTKERLAAMFLRT"
     gene            4996..>5966
                     /locus_tag="O0X12_02420"
     CDS             4996..>5966
                     /locus_tag="O0X12_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HJK53047.1"
                     /translation="MPEQKIVELYHTVQRRKVLVFGALLVLLLITSVLACGIGTVAIP
                     PADVLAAFGHAILPGLIDAPATPNAEFIITGYRLPRILLAVLAGVSLAVAGAVMQGLL
                     RNPLVSPFTLGLSSAASFGAAFMIVLGPLLFGAAFAGAVTVAGISFSLDTALLILSAF
                     LFGWLSVVLVYLISRTKQTSQAIMILAGVVIGYLFQAGLLALQYVSDDEALRDIVTWL
                     MGGMWGANWQAVLVLLPIVLICFILVERRAWDLNTLSGGDDVAKNLGINVPQFRIRGL
                     VIVSFAASACLAFTGVIGFIGLMAPHICRMIVGNDYRYLLPASAVMGA"
BASE COUNT         1310 a         1609 c         1794 g         1253 t
ORIGIN      
        1 cagcagaacc ctgatgcagc tgcttgccga gctggacggg tttgacaacc gcggcgatgt
       61 gaaggtggtg ggtgcaacga accggcccga tattcttgac cgggcgcttc tgcggccggg
      121 acggtttgac cgcatcattg agttccctct gccggatgag gagggacgac gaacgattct
      181 tgcaattcac acacgcaaaa tgaatctgcg aaagacggtg tccctggcag agattgcaaa
      241 ggagaccgag ggaatgaacg gctcggagct gatggcagtc tgtgtggagg cgggaatgaa
      301 tgcgatccgc agcagacgga cgacgattac gcaggatgac tttgacaagg cacttgcagc
      361 ggtaaagacc ggcaggtccg gtgttgtggt tcaggcaccg gatgcaatgt actcctgatt
      421 tttgggagac accaaaaaac cctcggtttt tgggcaggat cggaaaaaat gattctgttt
      481 tgtgggacag cagcggatgc gggaggggcg gatccggtcc aaaccccacc ttttcaatag
      541 cagaaaaacc catacatact actcatctat ggttccccag aagaagctca ctcctgcaat
      601 ggaacaggtc agaatgttca aggaacagta tcctgactgt attctcttta tgcggatggg
      661 tgacttttac gaaacattct gggaggacgc cgagatctgt gcacgcgagc tggatattgt
      721 ccagacctcg cggtccaagg atccggacgg caacccgatt cctcttgcag gtattcccta
      781 tcatgcactg gatctgtatc ttccccggat gatccgcaag ggatacaaga ttgccatctg
      841 cgaacaaatc gaggatccca aaacagcaaa aggctgtgtg aagcgggatg ttatccgtgt
      901 tgtgactccg ggaacggcga ttgacagcgg tatcgttgac accagtgcgg cacattattt
      961 aatggcggtt gcggctgata caaaaggcag agctggactt gcatttctgg atattaccac
     1021 cggcgagttt ttcgtggagg atatcccgct ggaggagaac aacgcctcgc ttgcaaccga
     1081 gatagaacgt tacacgccgg cggagattct ggtgccgcag cagattccgg caaatcttct
     1141 cactttcctt gccggatgcg gccgggtcat cacccccgga cgggctggcc tgtttgacga
     1201 cggagaacag gcgctgtgta ctgcattcgg cgttgcgtcg cttgacgggt atgactgtgc
     1261 cgattctccg ctctgcatca gtgcagcagc agctgctctc aggtatgcaa aggagacaca
     1321 aaaaaccgct cttccgcaca tcagcggatt tttccgcagg cattcaggcg atgcaatggt
     1381 gctggatgcg atcaccctgc gaaatcttga gatcctctcg cctctccggg gagaccggaa
     1441 cgatacaacg ctgttcggct ttctgaaccg caccaagaca ccaatgggca gccggacgct
     1501 gagaagtact gttaccgtgc cgctgctgga tgcggctacg atcaaccggc ggctggatgc
     1561 ggtggagttt ttcacaaacc ggccggttct gtgtaagact ctggacgggg tgcttgccaa
     1621 agttgccgac attgaacgga ttgcgggaag gattgcctac ggaaacgcat ctccgcggga
     1681 tctgctctcg cttgcggcaa gtcttagcat cctgccggag atcgaaaagg agatcggtaa
     1741 aacctccggc cttctggcag aacagcttgc ccgcatccct gatttttccg gggttgccgg
     1801 actgattact tccgcaatca tcgaggatcc gcctgcggtg taccgcaacg gcggcgtcat
     1861 ccgtgcggga tacaatgcag atcttgacga gctcaggacg gtggttgcaa ccggacggga
     1921 ctggatcgca gagcttcagc agagtgaacg cgaccggacc ggtatcaagt cgctgaagat
     1981 cgcctacaac aatgtcttcg gctactacat tgaggtgacc aaggcgaaca tcagcaaggt
     2041 cccgcccgag tacgaacgca aacagaccac ggtaaacggc gaacggttca ctcttccggc
     2101 actccgcgag cgggaagccc tgatggcgca ggcggacgac cggatccttg cactggaggt
     2161 tgcactgtat gaggcgcttc ttgcaacact gcgtgaccat gtaccggaac tcaaggaggc
     2221 ggcggaagga atcggaacga tcgacatgct cgtctcgttt gcagagcttg cgtccgcaaa
     2281 ccggtatgtg cggccagagt tctccgcggg agccgatctg ctgatccgtg acggccgcca
     2341 cccgatcgtc gaggactcgg ttccgggcgg ctacgtcccc aacgacaccg agatgaacgc
     2401 gtcaggggat cagattctga ttctgaccgg cgcaaacatg gccggaaagt caacctacat
     2461 gcgaagcgtc gcgttaatct gcatcatggc acagatcgga tcttttgttc cggcacgcta
     2521 tgccaggatc gggatggtgg atcggatctt cacccgcgtc ggggcgtcgg atgatcttgc
     2581 cggaggccag agtacgttca tggttgagat gctggagctg gcaaacattc tcaacaatgc
     2641 aacagaccgg agtctgatcc ttctggacga gatcggacgc ggcacaagta cggtggacgg
     2701 gtatgcgatc gcccgtgcgg tgctggagtt cctgcacggc aaaggagcat ccggtccccg
     2761 gacgctgttt gccacccact tccaccagct gatcggcatg gagcaggagt tccgcagaat
     2821 gaaaaactat cacttcgccg taaaggagga tcagcacgat atcacgttcc tgcgaaagct
     2881 gattccagga gcaacggatc gaagttacgg tattcatgtg gcacggattg cgggcgttcc
     2941 gaagaaggtg ctgacgcggg cggaggcgat tttgaatcag gcactccgtg aggatgccgg
     3001 ttccggcggc aggaggtact acacgcagat gcttctcgtg gacacgaccg cagaaccgct
     3061 gacgccggag gtgtccgcgg tggaagagcg ggttcgcgag gcaaacatca acgaaatgac
     3121 gccgatgcag gcgctgatgt ttatcaatga actcaaatcc atgctggaga ggaagtaatg
     3181 ggcgtaatta ccgttcttga tgaggagacg atcagccaca ttgccgcagg cgaggtggtg
     3241 gagcgggcgg cgtcggtggt aaaggagctg gtggagaatg cggttgacgc cggagcgaaa
     3301 cggatcaaaa tcgatcttgc tgcggacaaa acagcggtaa ccagaattgc ggtcaccgac
     3361 gacggctgcg gaatggatga cgaggacgca ctgctggcgt tccgccagca tgcaacaagc
     3421 aagatatcgc ggcccgaaga cctcgcatcc atcggaacgc tcgggttcag gggcgaggca
     3481 atggcaagta ttgcggcggt ttcacaggtg acgctgacca ccaaaaagca gggtgcaaaa
     3541 acgccggagg caacccgtgt ggtggtgcac ggcggcgaac tggtcgagca caccgcaacc
     3601 ggcgcaccgg aaggaacgac ggtcattgtt gagggactct tttacaacac acctgcacgg
     3661 cgtaagtttc agaagacggt tgcaaccgag cttgcccaca tctatgatat ggtggaacgg
     3721 atggcgctgg cgcaccgcga catctccttt gtactgtcct atcagggaaa ggaacggttc
     3781 cgcacctttg gcaacggcag ttttgaggat gtgattgcag cggtgttcgg gcagtcgttt
     3841 gccaaagaac tggtgccggt gaaaggaacc tacgggattg taacggtttc tgggtttgtg
     3901 acaagaccgg gttctgagat gaagagtacc ccaagccggt tttatctttc gattaaccgg
     3961 cggcagatat cctcgcgtcc gctacagtgg gcggtgcgtg aaggatacgg gacgcttctt
     4021 ccaaaaggga tgtatcctgc ggcatttctg gatctggaga tcaacccgtc cgatgtggac
     4081 gtgaacgtgc accctaccaa aaaggaggtg cggctgtccc gcgaacggga ggtgcagacc
     4141 ggtgtgcagg atgcggtgta ctgtgccctg catgagacgc gggtgtttgc agcagcggat
     4201 caacagagcc acaccgaacc acggaaagat tccgggaaaa atgttgtatc aatgctgccg
     4261 ttctcggttc tgggcgagtc gcagtccccg tacgagcggc ggattacccc gcagaaaaag
     4321 gagaccgcag caccaaagat tcaggcagcc gcactccgcc agacggaccg gcagctgcgg
     4381 cggacagagc ggtttgatcc tccggagacc gcggagtttg tgcccgaggt tctgggacag
     4441 attgcggaca cctacattct tgcacgcaac gaaaccggcg atctggtggt gatcgatcag
     4501 catgcagcac atgaacgggt gatgtatgat ctcctgcttg cccggcagga ggcaggactc
     4561 tccggtcagg aactgcttgt tcccgtgcag ctgaaactca caaaaaaaga ggcggcagca
     4621 gttccggatc tgcttccggt tctcgcggat gccggataca ctcttgaacc gttcggaaag
     4681 gatgtctgga tggtacgtac cgttccggtg gtctcggcat cgcttggtga tcccgcggtg
     4741 attcacgaga tcattgccca ggcactggac aaaacatcct caggcggcga ggaaagtgtc
     4801 cttgaccgtg tgttaaagac cgctgcctgc cgggcggtgg tgaagggggc aaccccgatg
     4861 acaaccgaac agatgcagcg tctgctccgc cagctgatgg caacccgatc tccgtacacc
     4921 tgtccgcacg gcaggccgac aacgattgta ctgacaaaag aacgacttgc tgcaatgttt
     4981 ctgcggacgt gaaccatgcc ggaacaaaag attgtcgaac tctatcacac cgtccagagg
     5041 agaaaggtcc ttgtttttgg agcactgctt gtccttctcc tgattacctc tgttctcgcc
     5101 tgcggaatcg gaacggttgc catccctccg gcagatgtac tggcggcctt cggccatgcg
     5161 attcttcccg gactcataga cgcacccgcc acacccaatg ccgagttcat catcaccggc
     5221 taccggctcc cccgtatcct gctcgccgtt cttgcaggtg tgagtcttgc cgtcgcagga
     5281 gcggtcatgc agggactgct ccgcaaccct ctggtcagcc cgttcaccct tggtctctcc
     5341 tctgcggcat cgttcggtgc ggcgttcatg atagtactcg ggccgcttct cttcggcgca
     5401 gcatttgccg gagccgtaac cgttgcaggg atcagcttct cccttgacac cgcacttctc
     5461 atcctctccg catttctctt tggctggctg agcgttgtac tggtatatct catctcccgt
     5521 acaaaacaga cctcccaggc catcatgatc ctggcaggcg ttgtcatcgg ttatctgttc
     5581 caggcaggac tgcttgcttt gcagtatgtc tccgatgatg aggcactccg cgacatcgtt
     5641 acctggctta tgggcggcat gtggggagca aactggcagg cagtacttgt tcttctgccg
     5701 attgtgctca tctgtttcat cctggtcgaa cggcgggcat gggatctcaa caccctttcc
     5761 ggcggcgacg atgtcgcaaa gaatctcggt atcaatgtcc cgcagttccg gatacgcggt
     5821 ctggtgatcg tcagttttgc cgcgtccgcc tgtcttgcat tcaccggcgt gatagggttt
     5881 atcggtctca tggccccgca tatctgccgg atgattgtgg gaaacgatta ccggtatctc
     5941 cttccggcct ccgcagtgat gggagc
//
LOCUS       DALTZZ010000128         3175 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_19656,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000128 DALTZZ010000000
VERSION     DALTZZ010000128.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19656"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            124..585
                     /gene="ribC"
                     /locus_tag="O0X12_02425"
     CDS             124..585
                     /gene="ribC"
                     /locus_tag="O0X12_02425"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HJK53048.1"
                     /translation="MIIGVVDTTFARANMGAFAVDELKKHTSARIIRVTVPGVKDLPV
                     AAKKLIEEERCDIVMALGMPGGKDQDKICAHEASQGIMMAQLMTNKHIIEVFVHEDEA
                     KDDAELAWLLEQRTREHAVNAVLMLTRPQDMTKLAGTGQRQGFADVGPARR"
     gene            642..827
                     /locus_tag="O0X12_02430"
     CDS             642..827
                     /locus_tag="O0X12_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53049.1"
                     /translation="MKNVEMNLEGNILTIKVDITKEFGVSGSGKSIIIASTEGNQSIA
                     PDHDVKIGLNVYRKNTN"
     gene            920..2317
                     /locus_tag="O0X12_02435"
     CDS             920..2317
                     /locus_tag="O0X12_02435"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HJK53050.1"
                     /translation="MRLVMKFGGTSVGDEKCIANVVNIIKESREAGNEIAVVVSAMTR
                     VTDQLIATAEEVINCKQKPALDAFITSIRSRHMKVLEAVAPDYVDEIGEYLDSRLNKL
                     SNILLAVHNLRELTPRSKDYIISFGERLSAPIISAALRQAGIPSCYMSGCDAGILTDG
                     VHNGATALPESYARIQARVGALLTEEVPVVMGYMGCAADGAVTTLGRSGSDYSGAIVG
                     AGIDADEILIWTDVDGVMTTDPRIIPEARVINSISFLEMMEMSYFGAKVIHPRALVPA
                     MQKNIPVRVKNTFNPSHPGTVVIREPHADKRIVKAISLIQNSCLIKVGGAVMAGRPGV
                     AGEIFTTLAEAGVNVMLISQGSSEMTISMIINEEQLDAALSALAEVKQRGCIREFGFD
                     RDVAVVSVVGAGMAGTYGTLGRIFHGLGVAGINVMMLSQGSEVNVSFVVKRADGVKAV
                     RAIHEEYHLEEEEEA"
     gene            2314..>3175
                     /gene="purM"
                     /locus_tag="O0X12_02440"
     CDS             2314..>3175
                     /gene="purM"
                     /locus_tag="O0X12_02440"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HJK53051.1"
                     /translation="MSTKYSYKDAGVDINLEADGVKALIHQLSYKRSGEHGMLGGVGH
                     FAGLIDFGSKVLSLCTDGVGTKMRVADDLGDWTTVGIDCIAMNVNDMYVMNIEPVAFV
                     DYIATEGINTEQMIQIGIGLNEGAKRANMNIVGGETATLKGMINGLDLAGTCLGVQDR
                     DRVVTGEKVVPGDVIIGVASTGVHSNGYTLARKIATENGGYDVRLPSGKTVGEALITP
                     TRIYSEVLDVCRAAEVHGMCHVTGGGLLNFLRISDYGFSIEDPMPVPEILQWIKEKGE
                     LETNELYRTFN"
BASE COUNT          822 a          790 c          874 g          689 t
ORIGIN      
        1 catgggcatc attgatacgc cggaaattct tgcaaccgtt gcagcagtac agatggcatt
       61 caagaagacc ggatacaaac tcgaaggcga cggtcttgcc gctgcagtcg aggttctgtc
      121 cgcatgatca tcggcgtagt cgacacaaca ttcgcccgcg ccaacatggg cgcgtttgcc
      181 gttgatgagc tgaaaaagca cacaagtgcc cggatcatcc gtgtgacagt gccgggagtg
      241 aaagatctcc cggtcgccgc aaaaaaactc attgaagagg aacgctgcga cattgtcatg
      301 gcgcttggca tgccgggcgg aaaggaccag gacaaaatct gtgcccatga agcctcgcag
      361 ggtatcatga tggcacagct gatgacaaat aagcacatca ttgaggtgtt cgttcacgag
      421 gacgaggcaa aagatgatgc agaacttgcg tggctgctgg aacagcggac ccgcgagcac
      481 gcggtcaacg ctgtcctgat gctgacacgc ccgcaggata tgacaaaact cgccggaacc
      541 ggccagcgtc agggatttgc tgacgtgggt cctgcccgga gataactttt tttttgaaaa
      601 atttcaggaa aggctatata atttcctgca gaggtactct tatgaaaaat gtagagatga
      661 accttgaagg aaatatcctg accattaaag tagatatcac caaagagttt ggtgtatccg
      721 gttccggaaa gtccatcata attgcatcca ccgagggaaa ccagtcgatt gcccctgacc
      781 acgatgtcaa aatcggctta aacgtgtacc gcaagaacac caactaactc ttttttcaga
      841 caccacactc ccgtggacaa tttatatcac ctcacaacac ccatctgata gaacagtatt
      901 atcataggtg ctaaatccga tgcgactcgt aatgaagttt ggaggaacat ccgtcggcga
      961 tgaaaaatgc attgccaatg tggtcaacat catcaaggaa tcccgcgaag caggaaacga
     1021 aatcgcagta gtggtctctg ccatgactcg cgtaaccgat cagctgatcg caaccgccga
     1081 agaggtcatc aactgcaaac aaaagccagc ccttgatgcg ttcatcacat cgatccgcag
     1141 ccggcacatg aaggttctgg aagcggttgc cccagactat gtggatgaga ttggggagta
     1201 tctcgattcc cggcttaaca aactttccaa catcctgctg gcagtccaca acctccgtga
     1261 acttaccccg cggtccaagg actacatcat ttcgtttggt gagcggttgt ccgctccgat
     1321 catcagcgca gcactccggc aggcaggtat tccaagctgt tacatgagcg gctgtgatgc
     1381 aggaattctg acagacggtg tgcacaacgg cgcaactgca cttccggaaa gctatgctcg
     1441 gattcaggca cgcgtcggtg ctcttctgac cgaggaagtt cctgttgtga tgggatacat
     1501 gggatgtgcg gcagatggtg cagtcaccac tctgggacgc agcggttccg actactcagg
     1561 agcaattgtg ggtgcaggca tcgatgccga tgagattctc atctggacag atgttgacgg
     1621 tgttatgacc acggacccac gcatcatccc ggaagccagg gttatcaact cgatctcttt
     1681 ccttgagatg atggagatgt cctacttcgg cgcaaaggtc attcacccgc gggcactcgt
     1741 accggcaatg cagaaaaaca tcccagttcg tgtcaagaac accttcaatc catctcaccc
     1801 tggtacggtt gtcattcgtg agccgcacgc agacaagagg attgtaaagg caatctcctt
     1861 gatacagaac agctgtctga tcaaagtcgg cggagcggtc atggcaggac gcccaggtgt
     1921 tgccggagag atcttcacga cgcttgctga agccggtgtc aatgtaatgc tgatctcgca
     1981 gggatcatcc gagatgacca tctcaatgat catcaacgaa gagcagttgg acgccgcact
     2041 ctcggcactt gcagaggtga agcagagagg atgcatccgt gagtttggat ttgaccgtga
     2101 tgttgccgtc gtttcggttg tcggtgccgg aatggcagga acctatggaa cgctgggacg
     2161 tatcttccac ggccttggcg tcgccggtat caacgtgatg atgctttcac aaggatcgga
     2221 agtgaacgtt tccttcgtgg tcaaacgtgc tgacggagtg aaagcggtcc gggcgatcca
     2281 cgaagagtac cacctggaag aagaggaaga agcatgagta caaaatattc ctataaggac
     2341 gcaggtgttg acatcaacct tgaagccgac ggcgtcaagg cactgatcca ccagctgagt
     2401 tacaaacgct ccggcgagca cggtatgctt ggcggagtgg gacactttgc cggtctgatt
     2461 gattttggat ccaaagtcct ctccctctgt accgatggtg tcggaacaaa aatgcgggtt
     2521 gccgatgatc tcggtgactg gacgacggtc gggattgatt gtattgcgat gaacgtcaac
     2581 gacatgtacg tgatgaacat cgaaccggtg gcattcgtcg actacatcgc aaccgaaggc
     2641 atcaacaccg aacagatgat ccagatcggt atcggattga acgaaggtgc gaaacgtgca
     2701 aacatgaaca ttgtcggcgg ggagacggca acgctgaaag gaatgatcaa tggtctggat
     2761 ctggcaggaa cctgtcttgg cgtgcaggat cgcgaccgag tggttaccgg tgagaaggtt
     2821 gttccgggag acgtaatcat tggtgttgca agtaccggtg ttcactcaaa cggctatacc
     2881 ctggcaagaa agatcgcaac cgaaaacggc ggttacgatg tcagacttcc ttccggaaag
     2941 acagttggcg aagcactcat tacgccaacc cgcatctact ctgaggttct ggacgtctgc
     3001 cgtgcggccg aggtccacgg catgtgccat gtaaccggcg gcggtctctt aaatttcctg
     3061 cggatcagtg actacggatt ttccattgag gatccaatgc cggttccgga gattttacag
     3121 tggatcaaag agaagggcga acttgagacg aacgagctgt accggacgtt taaca
//
LOCUS       DALTZZ010000129         2591 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_19759,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000129 DALTZZ010000000
VERSION     DALTZZ010000129.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19759"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1168
                     /locus_tag="O0X12_02445"
     CDS             <1..1168
                     /locus_tag="O0X12_02445"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HJK53052.1"
                     /translation="AEDIRRRAAYARSRVETLRCQLPPGYSSSSDLEASVRKMEEEIT
                     LHRKRETDLIAACDAAKTAMYDAVSACTAAERIRSEAKMLCAADDEALSHAVAERGLD
                     PLNLRTDLMTEEEYSRAEQMLEQYRKDCSSTDAEVVLLRKSTEGYISVDISALSSALS
                     GVEEEYAKLQGQEKAIEYRILQNTTAISDISSLLEQYRAAAAEYTELSDLSRAANGDA
                     AGTAVRMKFEEYVQSAYLSRILEKANIRLHAMTDGRFSIVQRSSSTDLRKKEGLEMDV
                     IDCYTQKQRPTSTLSGGESFKAALSLALGLSDVIMESSGGIELDALFIDEGFGSLDAV
                     SLDQAIETLATLALPRSGSRLIGIISHVEDLRQRIEKKILVVKKPDGSSAKVLV"
     gene            complement(1233..2444)
                     /locus_tag="O0X12_02450"
     CDS             complement(1233..2444)
                     /locus_tag="O0X12_02450"
                     /EC_number="4.1.2.43"
                     /EC_number="4.2.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,6,7,8-tetrahydromethanopterin
                     hydro-lyase/3-hexulose-6-phosphate synthase"
                     /protein_id="HJK53053.1"
                     /translation="MFLIGEALVGDGAELAHIDLMIGDKMGPVGTAFANGLTQLSAGH
                     TPLLGVIRPNLLPKPAVLIVPKVTLKHTEQVTQIFGPAQAAVSKAVADALEDGVFTGM
                     DVDDVVIVASVFISPEAKDFNKLYRFNYGATRLALSRALDKFPDTATVLKEKDRAAHA
                     VMGFKVQRLWNPPYLQVAMDLVDMKQVERVLREVPQNDHVIFEAGTPLIKQFGLHVIG
                     EIRKIRPNCFIVADLKTLDTGNLEARMVSNAGGDAAVVSGLAPIETIAAFIKEAKKCG
                     IYAIIDMLNVAEPAKLIENLAKMGGAALLPQYVEMHRAIDKEASGEYSWGDIKKIKAV
                     AAKHNAKILVATAGGIRVPVVKKAIAAGADVVVVGRAITASKDIKNAAESFLAELDSE
                     EIDQFRVMTDF"
BASE COUNT          591 a          681 c          678 g          641 t
ORIGIN      
        1 ggcagaggat attcgcagac gtgccgcata tgcaagatcc cgtgttgaaa cgctgaggtg
       61 tcagcttccc cctggctact cttcctcatc cgatcttgaa gcatcggttc ggaaaatgga
      121 ggaagagatc actctccatc ggaaacggga aacggatctt atcgccgcct gtgatgcagc
      181 aaagaccgcc atgtatgatg ccgtatctgc ctgcaccgct gccgaaagga ttcgcagtga
      241 ggcaaaaatg ctgtgtgccg ccgatgatga ggcactttcc cacgctgttg cggaacgcgg
      301 gctggacccc ctgaatctcc gtacagacct catgaccgag gaggagtact cccgtgctga
      361 gcaaatgctg gagcagtacc gcaaggactg ttcctccacc gatgctgagg ttgtcctcct
      421 tcgaaaaagt accgagggtt acatatcagt agatatttct gccctttctt ctgctttatc
      481 tggggtcgaa gaggagtatg ccaaacttca ggggcaggaa aaagcgattg agtaccggat
      541 tttacagaac accaccgcaa tatctgatat ttccagtctt ctggaacagt accgggctgc
      601 tgccgctgaa tatactgagc tgtctgatct ttccagggct gcaaacggtg atgccgcagg
      661 cacagccgtt cggatgaagt ttgaggagta cgtgcagtcc gcgtacctct cccgcattct
      721 ggaaaaggca aacattcgtc tgcatgccat gaccgacggc cgtttctcca ttgtgcagcg
      781 cagcagttcc actgatctcc ggaaaaagga ggggcttgag atggacgtca tcgactgcta
      841 cacccagaag cagcgtccga cttccaccct ttccggcggc gagtcgttta aagcagcact
      901 atctctcgcc cttggtctgt cggatgtgat catggaaagt tccggcggca ttgagcttga
      961 tgccctcttc attgatgaag gattcggatc tttggatgct gtatctctgg atcaggcaat
     1021 tgagacactt gcaacccttg ccttgccaag aagcggaagc aggcttattg ggatcatctc
     1081 acatgttgag gatctgcggc aaaggattga aaagaagatt cttgttgtta aaaaaccgga
     1141 tgggagctct gccaaggttc tggtctgaac ctttttttcc ttttgtcagc gtttaaaagt
     1201 ttagactaaa aaagaaaaag aggaaatctg ttttagaagt ctgtcatcac gcggaactga
     1261 tcaatctctt ctgagtcaag ttctgcaagg aaggactcag ctgcattctt gatgtctttg
     1321 cttgcagtga tagcgcgtcc gaccactaca acatcagcac ctgctgcaat tgccttcttc
     1381 acaaccggca cacggatacc gccggcggtt gcaaccagaa tctttgcatt gtgctttgcg
     1441 gcaactgcct tgatcttctt gatgtcgccc cagctgtact cgccgcttgc ctctttgtcg
     1501 attgcgcggt gcatctcaac atactgcgga agaagtgctg ctcctcccat ctttgcaaga
     1561 ttctcgatca gctttgccgg ttctgcaaca ttgagcatgt cgatgattgc atagattccg
     1621 cacttctttg cctctttgat gaaggctgcg attgtctcga tcggtgcaag gccggagacc
     1681 actgctgcat cgccgccggc gttggagacc atacgggcct caaggttgcc ggtgtccagg
     1741 gtcttcaaat ctgcaacaat gaagcagttc gggcggatct tgcggatctc accaatcaca
     1801 tgtaagccga actgtttgat aagcggggtt cctgcctcaa agatgacgtg atcgttctgc
     1861 ggaacctcac gcaggacacg ctccacctgc ttcatatcga caaggtccat tgcgacctgc
     1921 agatacggcg ggttccagag ccgctgaact ttgaatccca taactgcgtg ggctgctctg
     1981 tccttctcct tcaggacggt tgcagtgtcc gggaacttat caagcgcacg ggagagtgca
     2041 aggcgggttg ctccgtagtt gaagcggtag agcttgttga agtcttttgc ctccggagag
     2101 atgaaaacac ttgcaacaat gaccacatca tccacatcca taccggtaaa gacgccgtct
     2161 tccagtgcat cggcaactgc tttggagact gcggcctgtg ccgggccgaa gatctgggtt
     2221 acctgttcgg tgtgcttcag cgtaaccttc ggaacgatca gaactgccgg ttttggcaga
     2281 aggttcgggc ggataacgcc aagcagcgga gtgtgtccgg cggagagctg ggttagaccg
     2341 tttgcaaaag cggttccgac cggccccatc ttgtcgccga tcataagatc gatgtgagcg
     2401 agttctgctc catcgccaac gagtgcttca ccaatcaaaa acatagaaaa tctcttctgt
     2461 aactttggtc ttgcaacact ataaagacga cgtggcattt ccgccaagtc ggactactgc
     2521 cgatccgttt ttgccagttc cgacaatgta tcgccacaga tgcggtacac agtccagtcg
     2581 ctcatcggta c
//
LOCUS       DALTZZ010000130         1746 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_19867,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000130 DALTZZ010000000
VERSION     DALTZZ010000130.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19867"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            216..1169
                     /locus_tag="O0X12_02455"
     CDS             216..1169
                     /locus_tag="O0X12_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53054.1"
                     /translation="MWHHEYIDKYVDTLVFDPAENTVSWVLDGICAADSISDEVLLAY
                     CRRSPLWFYSPLLRHDDGTVRFSDIHALAVHEAETNDILMTTFGRYRCCTINLALHPC
                     ETPEGVVDELREDWDHVALSLMNAENSKTMNQNYGLLYLMPGRLMTNEPYQDGIPLAP
                     KPVYTDRDKYIETLIFDPSNNTVSWMQDGICTADSVSEEALRECFRSHSLPFNSPRLS
                     DKDTFSFAALLEWGRQSAGKNMNEYALHIHTSTVGGLIFCTLNVLLHPIDGTNAFDPV
                     LDDWNQVKQIVHEADKKKTLAKNLPLLLTFAGYDAEGGRSL"
     gene            1200..>1746
                     /gene="cofG"
                     /locus_tag="O0X12_02460"
     CDS             1200..>1746
                     /gene="cofG"
                     /locus_tag="O0X12_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofG"
                     /protein_id="HJK53055.1"
                     /translation="MRSAPNLRTAMSRTITFSRNVFLPLTNVCANACGYCSFKAPVTD
                     GCVMPKTEVLATLRRGAEFGCTEALFTFGERPENEPGFRRYIEAAGYSDILSYCEAMS
                     RAAIDLGMLPHTNAGILTYEELEHLRCVNASMGLMLETTAEIPAHRHSPGKDPAVRLE
                     MMENAGRLKIPFTTGLLLGIGE"
BASE COUNT          415 a          434 c          472 g          425 t
ORIGIN      
        1 cggttatgtg aagcagtttc ccttgcaaga ggtggatctg gctttgttac tgcaagtccc
       61 aacgaggatc cagtcgtatc aggtcatccg caagtcagac attgtcatcc gagaaatctt
      121 gatggaactc agatgcgtga tctaaatggc gatagtccac attgtcacta tctggtgtat
      181 ccaatgaatc ttgcaggaag tatgagtgtc tgagtatgtg gcatcacgag tatattgaca
      241 agtatgtgga cacgctggtc tttgatccgg cagaaaacac ggtgtcctgg gtgctggacg
      301 gcatctgtgc agcagattcc atctcggatg aggtgttgtt ggcgtactgc cgcagatctc
      361 cactgtggtt ctactcaccg cttctccggc atgatgacgg gactgtccgt ttttcggata
      421 ttcacgcatt ggcggtgcat gaagccgaaa cgaacgatat actcatgaca actttcggca
      481 gataccggtg ctgtacgatc aatctcgccc tgcatccgtg tgaaacgccg gagggggtcg
      541 ttgatgaact ccgggaagat tgggatcatg ttgctctgtc actcatgaat gccgagaaca
      601 gcaaaaccat gaaccagaac tatggtttgc tgtatctgat gcccggcaga ctgatgacca
      661 atgaacccta tcaggacggc attccccttg caccaaagcc ggtttacacc gatcgcgaca
      721 agtacattga aacactgatc tttgatccgt cgaacaacac cgtctcatgg atgcaggacg
      781 gcatctgtac cgcagattcc gtctccgagg aagcactgcg ggagtgcttc cgcagtcatt
      841 cactgccgtt caactctccg cgtctctccg acaaagatac ttttagtttt gcggcactgc
      901 ttgaatgggg caggcagtcg gcgggaaaaa acatgaatga gtatgcgctg catattcata
      961 cgtcaactgt ggggggcctt atcttctgca cgctgaatgt acttcttcat cccatagacg
     1021 ggacgaatgc atttgatccg gttctggatg actggaatca ggtgaaacag attgtgcatg
     1081 aagcggacaa aaagaaaacg cttgcaaaga atctcccctt gcttctgacc tttgccgggt
     1141 acgacgccga aggcggacgt tctctctaat cttttttttg ggaaaccgga caggttcata
     1201 tgcgttcagc acccaatctt agaacagcta tgtcccggac gattacgttt tcccggaatg
     1261 tattccttcc tctgacaaat gtgtgtgcaa atgcctgcgg atactgttcg tttaaggcac
     1321 ccgttaccga cggctgtgta atgccaaaga cggaggttct cgccacactg cgccgcggtg
     1381 ccgagttcgg ctgtaccgag gcactgttca cttttggaga gcggccggag aacgaaccgg
     1441 gatttcggcg gtatatcgaa gcggcaggat attctgacat cctttcctac tgcgaagcaa
     1501 tgagccgggc agcgattgat cttggaatgc tgccgcacac caatgcagga attttaacgt
     1561 acgaggagct ggagcatctc cggtgcgtaa acgcgagcat gggactgatg ctggagacaa
     1621 ccgcagagat tccggcacac cgccacagtc cgggaaagga tccggcagtc cgccttgaga
     1681 tgatggaaaa cgcaggtcgg ctgaagattc cgtttacgac cgggcttctg ctcggcattg
     1741 gcgaga
//
LOCUS       DALTZZ010000131         1866 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_19905,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000131 DALTZZ010000000
VERSION     DALTZZ010000131.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19905"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="O0X12_02465"
     CDS             complement(<1..451)
                     /locus_tag="O0X12_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HJK53056.1"
                     /translation="MQSFSNTLFADAETEYNDARYVIFGVPFDGTTSFKAGARDAPLA
                     VRAASYNLETYLPYYDIEMPEIGFHDMGDLYCDCLPDLVVDQVADAVSDLVKDEKIPV
                     MIGGEHSVTIGAVQTLAPAWYVVCDAHLDMQDEFRGSPYNHDCVTARV"
     gene            complement(469..849)
                     /locus_tag="O0X12_02470"
     CDS             complement(469..849)
                     /locus_tag="O0X12_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HJK53057.1"
                     /translation="MKEQTEVGKLKEGRYVVVDDEPCKIQSIAISKPGKHGAAKARID
                     VVGLFDGQKRSVVSPTSATIYAPIVERKTGQILTIAGNVVTFMDMKDFNNFELVVDDD
                     IVATFQPAQEVPYIESLGKRKLDI"
     gene            971..1762
                     /locus_tag="O0X12_02475"
     CDS             971..1762
                     /locus_tag="O0X12_02475"
                     /EC_number="3.1.3.11"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     fructose-bisphosphatase/inositol-phosphate phosphatase"
                     /protein_id="HJK53058.1"
                     /translation="MVTPFQFLSVCDEVGKMMVQELSPLVGTAYGGEELCIGADNTPT
                     ERIDRVAEDLVLSVFREKKICRSLLSEEAGMVDIGGDFGIAYLDPVDGTFNAVSGIPF
                     YALSIALSDGKQVVAGYVKNLANGETFTAILGEGAYLDGRPIQVSTETMLDHSAMSVY
                     AKKFDVTRMMQLGHKIRRWRLLGASALELCYVACGRLDGFVDLRNTLRVTDAAAGILI
                     CREAGGRVTLPDGSPVEFPDNVAAGRCLVATNRVIHRKVIEYLRS"
BASE COUNT          388 a          440 c          558 g          480 t
ORIGIN      
        1 caacccgtgc agtgacacag tcatgattgt agggggagcc acggaactca tcctgcatat
       61 ccagatgtgc gtcgcagacg acataccagg cgggggcgag cgtttggact gccccgattg
      121 ttaccgagtg ttcgccgccg atcatcaccg ggattttttc atccttgacg agatctgata
      181 ctgcatccgc cacctgatct accaccaggt ccggcagaca gtcgcagtag aggtcgccca
      241 tgtcgtggaa cccaatctcc ggcatctcga tgtcgtagta cggcagatac gtctcaagat
      301 tgtaggatgc ggcacggact gccagcggag catcacgcgc acctgcctta aacgatgtcg
      361 tcccgtcgaa gggaactcca aaaattacgt agcgtgcgtc gttgtattca gtttctgcat
      421 ccgcgaaaag agtgttagaa aaagactgca tggatgtatt gtgcagtctt agatgtcaag
      481 cttacgcttg ccaagggatt caatgtaggg aacttcctga gcaggctgga aggttgcaac
      541 gatatcatcg tcaacaacaa gttcgaagtt gttgaagtcc ttcatgtcca taaaggtcac
      601 aacgtttcct gcgatggtca ggatctgtcc ggtcttgcgc tctacgatcg gtgcgtagat
      661 ggttgcggac gtcggggaga cgacggaacg cttctgaccg tcaaagagac cgactacatc
      721 gattcttgcc tttgctgctc cgtgctttcc cggcttggag attgcaatgc tctggatctt
      781 gcacggctcg tcgtcaacaa caacgtaacg gccttccttg agcttaccaa cttctgtctg
      841 ttccttcatg ataattaact ctgactaatt atgtgaggta attgcttatg agtgttgcga
      901 cagatacgcg ggtgcctttt cgttcacccg ttgatttcat gaggctgccg cactaataca
      961 aatgtatcgt atggtaacgc catttcagtt tctttccgtc tgcgatgagg tcggaaaaat
     1021 gatggtgcag gagctttccc cgctggtagg tactgcctat ggaggtgagg agttgtgcat
     1081 cggtgcggat aatactccga cagaacgcat cgaccgcgtg gcagaggatc tggttctgtc
     1141 ggtgtttcgt gagaagaaga tctgccggtc gcttctttca gaggaggcgg gaatggttga
     1201 tatcggcggt gatttcggga tagcctatct cgatcctgtg gacgggacgt ttaatgcagt
     1261 ctccggaatt cccttctatg cgttgtcgat tgcgctttcc gacggtaagc aggtggttgc
     1321 agggtatgtg aaaaatcttg caaacggcga gacgttcacg gccattcttg gtgagggggc
     1381 ttatctggac ggcaggccga tccaggtctc gaccgagacg atgctggatc actcggcgat
     1441 gagtgtgtat gcaaagaagt ttgacgtgac gcggatgatg cagctcggcc ataaaatccg
     1501 ccgatggcgt ctgttagggg cgtctgcact tgagctctgc tatgttgcct gcggacggct
     1561 ggacgggttt gtggatctgc gcaataccct gcgggtaacc gatgctgcgg cgggtattct
     1621 gatctgccgt gaggccggcg ggcgtgtgac gcttccggac ggaagtcctg ttgagtttcc
     1681 ggataatgtg gcggcaggca gatgtctggt tgcgacgaac cgtgttattc accgcaaagt
     1741 gattgagtat ctgaggtcat gatgaaggtc tgtattgttt ccagagtgga tttccgggag
     1801 ccggttgaga ctgcccagtc gcttggctgg atgctggctg atgcggggca tgaggtggtc
     1861 tctgag
//
LOCUS       DALTZZ010000132         1993 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_19993,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000132 DALTZZ010000000
VERSION     DALTZZ010000132.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19993"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1727)
                     /gene="atwA"
                     /locus_tag="O0X12_02480"
     CDS             complement(<1..1727)
                     /gene="atwA"
                     /locus_tag="O0X12_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase system, component
                     A2"
                     /protein_id="HJK53059.1"
                     /translation="MTTPFITIKDLCMDFPANPEGPSDTTPCAAAQESVRVLDNINLE
                     IAEGEIVGIIGRSGCGKTVLIHLVRGIDQPPTSGQIIYHVAVCPKCGRIELPGHAGTA
                     CSNCGAVLEAQDVDFWAPENETLKTRIMARTAIMFQRTFALYGDDRVIENVLRALDDI
                     DYPSEKAISRAADLLDEVRLTHRMMHIARDLSGGEKQRVVLARQLARDPMFLFADEPT
                     GTLDPKTAKVVHDLLKNASREKNMGMLITSHFSQVLEDVADRAVLLDDGKITKIGTPE
                     EIIAAFMEGYHDDLVYADQEIGTPIVRAENLSKKFIAVDRGVINAVDKISFEINEREI
                     FGIIGVSGGGKTTLSKMIAGLYEPTAGKLEVRIGDEWIDMTKPGYLYRGRAKKYIGLL
                     HQEYDLYPHRTIIDNLTDAIGLEFPKELATRKAMITLQMAGFTEKKAKDVLHRYPASL
                     SEGEKHRVALAQVLIREPRIILLDEPTGTMDPITKVDVKHSIIHAREEMDETFVIVSH
                     DMEFVRDVCDRCMFMRGGKIIKIGPTHEVLAELTDAEKDIMARAVANERERAADNTPK
                     SAPTQADPKG"
     gene            complement(1847..>1993)
                     /locus_tag="O0X12_02485"
     CDS             complement(1847..>1993)
                     /locus_tag="O0X12_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53060.1"
                     /translation="VQYLQPKPERVFTIHGEESKTIDLASSIYKKYHIQTVAPQNLET
                     YRLV"
BASE COUNT          476 a          477 c          503 g          537 t
ORIGIN      
        1 cctttaggat cagcctgggt cggtgcagat ttcggagtgt tgtctgcagc gcgttcacgt
       61 tcatttgcaa ccgcacgtgc catgatatcc ttttctgcgt cagtgagttc tgcaagtact
      121 tcatgggtcg gtccaatctt gatgatcttt cctccccgca taaacataca gcggtcacag
      181 acatcacgga caaactccat atcatgtgaa acgatgacaa aagtttcatc catctcttcc
      241 ctggcatgaa tgatagagtg tttgacatcc accttggtga ttgggtccat cgtgcctgtc
      301 ggctcatcca aaaggataat gcggggttca cggatgagta cctgcgccag tgcgacacgg
      361 tgcttttcac cttccgaaag gcttgcagga tagcggtgaa ggacatcttt tgccttcttt
      421 tcagtaaatc ctgccatctg aagagtgatc atggccttac gggtggcaag ctcttttgga
      481 aattccaggc cgatcgcatc agtcaggttg tcaatgatgg tccggtgagg atagagatca
      541 tactcctgat gcagaagacc gatgtacttc tttgcccggc cgcgatagag gtatccgggt
      601 ttggtcatat cgatccactc atctccaata cgtacctcaa gctttcctgc agtgggctcg
      661 taaagtcctg caatcatttt tgacagagtg gtttttccgc caccggaaac accaatgatt
      721 ccgaatattt cgcgttcgtt aatctcaaaa ctgattttat ccacagcatt gatgacacca
      781 cggtcaaccg cgatgaattt tttggagagg ttttccgccc ggacgattgg agtaccgatt
      841 tcctgatcag cgtaaaccag atcgtcatga taaccttcca taaatgcggc aatgatctct
      901 tcgggtgtgc cgattttggt aatctttccg tcatcaagca gcacggcacg atcggcgaca
      961 tcttctaaaa cctgtgagaa gtgggatgtg atcagcatac ccatattctt ctcgcgggat
     1021 gcattcttga gaaggtcgtg gacaacctta gcggtttttg gatccagagt accggtcggt
     1081 tcatctgcga agaggaacat gggatcgcgg gcaagctggc gggcgaggac gacacgctgt
     1141 ttttctccac cggaaaggtc gcgggcgata tgcatcatgc ggtgggtgag acggacttcg
     1201 tcaagaagat cggctgccct tgagatggct ttttcagacg gatagtcgat atcatccaag
     1261 gcccggagga cgttttcaat gacgcgatcg tctccgtaca gggcaaatgt ccgctggaac
     1321 atgatcgcag tccttgccat gatacgggtt ttgagtgttt cattttccgg cgcccagaaa
     1381 tccacatcct gtgcttcaag cacagcaccg cagttactgc atgctgttcc cgcatgaccg
     1441 ggaagttcaa ttctgccgca tttgggacag acagcaacgt gataaatgat ctgtccggat
     1501 gtagggggct ggtcaatacc acgaacaaga tggatgagta cggtcttacc acatccggat
     1561 cgtccgatga taccaacgat ctctccttcg gctatctcaa gattgatgtt gtccaaaacc
     1621 cttactgact cctgcgcggc tgcacagggc gtagtatcgg aaggaccttc aggatttgca
     1681 ggaaaatcca tgcagaggtc ctttatggtg ataaacggtg tcgtcatgca aacatcctct
     1741 ataatgctaa atgtatgtgc tttgaggtgt tattagtgtt tagaagaatg cattctggcg
     1801 ggattattca caatgtgaca tcccagcctt gaaaaagaaa aaaagttcaa accagacggt
     1861 aagtctcaag attctgtggt gcaaccgtct gaatgtggta tttcttgtag attgaacttg
     1921 ccagatcaat tgtctttgac tcttcaccgt gaatggtgaa cacccgctca ggtttgggct
     1981 ggaggtactg cac
//
LOCUS       DALTZZ010000133         2593 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_20027,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000133 DALTZZ010000000
VERSION     DALTZZ010000133.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20027"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="O0X12_02490"
     CDS             <1..225
                     /locus_tag="O0X12_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJK53061.1"
                     /translation="KNNRGTISMANAGPNTGGSQFFINLVNNNYLDKAHPAFGTVVEG
                     MEVVDAIGKVKTNFADKPLEDVVIKKATLL"
     gene            346..972
                     /locus_tag="O0X12_02495"
     CDS             346..972
                     /locus_tag="O0X12_02495"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HJK53062.1"
                     /translation="MALKHEWRKEDKTLYLPKTQPELITIPAQKFFQLSGSGDPNNNP
                     TFAENIETLYALSYAVRMMPKQGYTPEGFEPYTVYPLEGVWGLVDPNSESKNKNNYSY
                     TLMIRQPEFVNEDVARLAIETVRKKKPHLSVNKVVFGAIEDGLSVQMMHVGPYDDEAA
                     SFVQMTTFLKENNLERTSPQHREIYLSDARRCTPEKQKTVLRWTVRHV"
     gene            complement(1007..2548)
                     /gene="hcp"
                     /locus_tag="O0X12_02500"
                     /gene_synonym="priS"
     CDS             complement(1007..2548)
                     /gene="hcp"
                     /locus_tag="O0X12_02500"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HJK53063.1"
                     /translation="MYCQQCQESKTPACTLGGACGKNKSVAEAQDRVLYSLSCLAFRA
                     KSANADLQPCVLEALFATLTNVSFDEERFEAYLSCITGHYNSLPPAAGEPAFDADAGL
                     DAISDANTRSLFSLLLYGVKGLAAYYSHALVLGKTDDTILPFIRDALASRFEDLSIDQ
                     RTALVLECGKQGVSVLALLDAANLTYGVPEITKVSTSSGVRPGILVTGHDLKDLAMLL
                     EATKDAGVDVYTHGEMLPAHAYPAFKKYPHLRGNFGGSWAEQRSDLAKFHGPILFTTN
                     CLVPPAPAYADKVFTTGAVGFPNCPHIDAKPDGSKDFSALIAAAKTCQPPEELGRPDL
                     ITGCAHDAVLALAPKVIELINEGKIRRFVVMAGCDGRDKRRSYYTEFAKVLPHDTIIL
                     TAGCAKYRYNALALGDIDGIPRVLDAGQCNDSYSLVVIALALADAFGCGVNDLPISYN
                     IAWYEQKAVLVLLALLHLGVKNIMLGPSLPEFVSPDVLNVLVKTFGLQPSSTVEEDLK
                     KLSCV"
BASE COUNT          747 a          609 c          662 g          575 t
ORIGIN      
        1 aagaacaacc gcggtacaat ctccatggca aatgccggtc caaacacggg aggcagccag
       61 ttcttcatta acctcgtcaa caacaactat ctcgacaaag ctcaccctgc ctttggtacc
      121 gttgtggaag gtatggaggt tgttgacgcc attggaaaag ttaagaccaa ttttgccgac
      181 aagccgcttg aagacgtggt gattaaaaag gcaaccctcc tctaaacaca tcagacagaa
      241 tatagtccct gaaaaggggc gcaaaaacta ttttttcatc acagttgcat tttcacaccg
      301 catggttggc tatttatatt cccaaacagg taatagagca aaacaatggc tctcaaacac
      361 gagtggcgga aagaggacaa aacactctac ctcccaaaaa cccagccgga actcataaca
      421 attcctgcac aaaaattttt tcagctatcc ggtagcggag acccaaacaa taatccgaca
      481 tttgctgaaa acatagaaac cctgtatgca ctttcttatg cagtccgcat gatgccaaaa
      541 cagggttaca caccggaagg gtttgagccc tacacggtgt atccgctgga aggtgtgtgg
      601 ggtctcgttg atcctaattc ggaaagtaaa aacaagaaca actattccta cacactgatg
      661 atacgccaac ctgagtttgt aaacgaagat gttgcaagac ttgcaattga aacggtcaga
      721 aaaaagaagc cacatctatc ggtgaacaaa gtggtgtttg gagcaataga agacggtcta
      781 tcagttcaga tgatgcatgt cggaccctat gacgatgagg ccgcaagttt tgtacagatg
      841 acaacgtttt tgaaagagaa caatctggaa cgaacatcac cacagcaccg cgagatctat
      901 ctgtcagatg cacggcgctg tactcctgaa aaacaaaaga cggtgcttcg ctggactgtc
      961 cggcatgtgt aagaattaat ggataaaaat gaagagggaa gggagatcac acacaggaca
     1021 gctttttcag atcctcttca acagtggagc ttggctgaag cccgaaggtc tttaccagta
     1081 cattcagcac atccggagaa acaaactctg gcagggaagg accaagcatg atgttcttta
     1141 cgccgagatg cagcagggca agaagtacga gtaccgcttt ctgctcatac caggcaatgt
     1201 tgtaggagat ggggagatca ttgacaccac aaccaaaggc atccgcaagt gcaagtgcga
     1261 tgacaacaag actgtaggag tcgttgcact gacccgcatc cagcacgcgg gggattccat
     1321 cgatgtcacc gagggcaaga gcattgtagc ggtacttggc acagccggca gtgagaatga
     1381 tggtgtcatg gggaagaact ttggcaaact cggtgtagta actgcggcgt ttgtcgcgac
     1441 catcacatcc tgccataacc acaaacctcc ggatctttcc ctcgttgatc agttcaatga
     1501 ccttgggggc aagggcaaga actgcgtcat gagcacaacc ggtaatcaga tctgggcgac
     1561 cgagttcttc aggaggttga caggtttttg cagctgcaat aagtgcagag aagtctttgc
     1621 taccatccgg ttttgcatcg atgtgggggc agttcgggaa gccgaccgca ccggtagtga
     1681 aaactttgtc cgcgtaagcc ggagctggcg gtactagaca gttggtggta aaaagaatcg
     1741 gaccatggaa ctttgcaaga tctgatctct gctcagccca cgaaccacca aagtttccgc
     1801 gaagatgcgg atacttcttg aacgctgggt atgcatgggc tggcagcatt tcaccgtggg
     1861 tgtaaacgtc aactcccgca tccttcgttg cctcaagaag catagcaaga tccttgagat
     1921 catgaccggt tactaaaata ccggggcgaa ccccagaact tgtagagacc tttgttatct
     1981 ccgggacccc gtaggtcagg ttcgctgcat caagaagtgc aaggaccgaa acaccctgtt
     2041 taccacactc aagaacaagt gcggtgcgct gatcgatgga gagatcttca aaccgcgatg
     2101 caagcgcatc ccggataaac ggaagaatag tatcatcggt ttttccaagg acaagtgcgt
     2161 gcgagtagta ggcggcaagg cctttcacac catacagcaa cagcgagaag agggaacggg
     2221 tgttggcatc actgatagca tcaagacctg catccgcatc aaaggcaggc tcacctgctg
     2281 ccgggggaag actgttgtag tgaccggtga tgcaggagag atatgcttca aaccgttctt
     2341 cgtcgaagct gacattggtc aatgttgcaa atagtgcttc cagaacgcac ggttgcagat
     2401 cagcgttagc ggattttgca cggaatgcaa gacaggagag ggagtagagc acacggtcct
     2461 gtgcctccgc aacacttttg ttctttccac aggctccacc aagggtacag gcgggggttt
     2521 ttgattcctg acattgttgg cagtacatgg attatcacca aggtttcttt tggatagtaa
     2581 gtatattgta aat
//
LOCUS       DALTZZ010000134         5363 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_20128,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000134 DALTZZ010000000
VERSION     DALTZZ010000134.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20128"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="O0X12_02505"
     CDS             <1..545
                     /locus_tag="O0X12_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HJK53064.1"
                     /translation="CGGLALGYKACELAAEKLGLHFSEDEEIVAVTETDSCTVDAIQV
                     LLGCTAGKGNLFVNNWGKTAFSFYRRDNNTSIRLVAVPDAVPKNARMVELRPKIMDGS
                     ATPAEDEEYHRLLHEHVHEILATPAEKLFIIKDAVQPLPKEAKIYNNVVCSVCGEQVA
                     DGLAQQSEGKYICIPCQMRK"
     gene            complement(834..1748)
                     /gene="cofD"
                     /locus_tag="O0X12_02510"
     CDS             complement(834..1748)
                     /gene="cofD"
                     /locus_tag="O0X12_02510"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HJK53065.1"
                     /translation="MITVLSGGTGTPKLIRGLRQILRDNEITVVVNTAEDLWMSGLYV
                     SPDIDTVQYLFSGLLNTDSWWGIRGDSFETFHAMEKLGYTEPLPLGDRDRATNITRAE
                     FLRQGITLTKATDKIAKAYGVQATILPMSDQEVTTYVRTEDGSLMHYQEYWVGRRGNV
                     PITGIVRKTAGDAPLTATPEVIAAIEESEGVIIGPSNPVTSIGPILECAGVREALRKK
                     FTIAVSPFIGSRPVSGPAAALMQAWGYEPTSYGTWEVYKDVVSMFIQDKRDTEIDVPG
                     AHRLDTMMTNEKKAESLAWDLLSYFPRK"
     gene            complement(1838..3175)
                     /locus_tag="O0X12_02515"
     CDS             complement(1838..3175)
                     /locus_tag="O0X12_02515"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HJK53066.1"
                     /translation="MNERGLFSSRMGFILAAAGAAVGLGNIWRFPYLAAEYGGGIFLL
                     VYLILVVTLGFTIMITEIAVGRSTGTSVVTAFAKLNRKYTFIGYLSLLVPLIILPYYS
                     LIGGWVMKYAAVYFMGQTDTAAADGFFSGFTSLTVEPILWTLLFVALACIVVVFGVEK
                     GIERANKILMPTLIVMLIGLTAYCLSLPGGMDGLWYYIYPDISKFSAETVLAAMGQVF
                     YSLTLGFGVMITYGSYLAKKTDMEKSVRTIEIFDTGVAFLAGLLIIPAVFVFSGGSPE
                     ALGAGPGLMFQALPKVFAMMPFGSAVAAIFFVLVAVAALTSCICMLEVPVAVLGDRFG
                     VSRKTASLLVFLFTMALAVPISLGFGMLDWISVAGMSLLDMFDFFSNNILLPIVAILT
                     CIFIGWIVKPKVVIDEVKSSSPFKAERMYVVMVKFIAPICIAAILIYGLLSVL"
     gene            complement(3226..4626)
                     /gene="purF"
                     /locus_tag="O0X12_02520"
     CDS             complement(3226..4626)
                     /gene="purF"
                     /locus_tag="O0X12_02520"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HJK53067.1"
                     /translation="MSGIAGIVDSSDVAYPLYYALHALQHRGQEAAGISTFHEKTLLI
                     HKGPGQLSEVFNEQILSKLPGTVGIGQVLYTQKAHRGKTENIQPLKFSFQGHELAITV
                     SVALVQDNRESLRAEYEGKGHIFSTTTNAELIAAMIAHELISGADAEDAFVNAIRRLK
                     GAYAGVAILDGVLYAFRDPLGTKPLCLGKLDTGYIVASESVAIDTLSGEFLRDVTPGE
                     LVIITDNGISSRQVLTADHKAYCVFEYVYTARPDSVIDGVLVYDARRRIGEKLAKHPV
                     KADLVSPVPDSGTAFATGFADASGIPYMEGLLKNRYVGRTFIMPAQSLRENAVRMKLN
                     PVRRHISGKSVILVDDSIVRGTTSLHIVDMVREFGAAEVHMRIGSTPIVAPCYFGVDL
                     PTREELIANGRTVEEIRDMIHATSLEYISTEDLVESVGIPSEDLCMACSCGAYPLEIP
                     NESCCACRRITPAKDD"
     gene            complement(4639..4812)
                     /locus_tag="O0X12_02525"
     CDS             complement(4639..4812)
                     /locus_tag="O0X12_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L37e"
                     /protein_id="HJK53068.1"
                     /translation="MTKGTPSMGLRNKHSHIICRRCGKQSFHARHGVCSSCGFGKSSK
                     IRGYKWTKKAADN"
     gene            complement(4830..5057)
                     /locus_tag="O0X12_02530"
     CDS             complement(4830..5057)
                     /locus_tag="O0X12_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HJK53069.1"
                     /translation="MTKRPLEILDQVLNRQPVIISLKGGREIRGVLQGYDVHMNLVLD
                     KAEEEGENGTVQLGTLIVRGDNVIYISPSVE"
BASE COUNT         1326 a         1490 c         1286 g         1261 t
ORIGIN      
        1 cctgcggagg gcttgcactc gggtacaaag cctgcgaact tgccgcagag aaacttggcc
       61 tacacttctc agaggatgag gagattgtag cagttaccga gaccgattcc tgtaccgttg
      121 atgcaattca ggtactgctc ggctgtaccg ccggcaaagg gaatctgttc gtaaacaact
      181 ggggtaagac ggcgttctcg ttctatcgcc gcgacaacaa cacctccatc cgccttgttg
      241 ccgttccgga tgctgttccc aaaaatgcac gcatggttga gctccggccg aagatcatgg
      301 acgggtcggc aacgcctgcc gaggatgagg agtaccaccg ccttttgcat gaacatgtgc
      361 acgaaattct tgcaacgcct gcggaaaaac tgttcatcat caaagatgct gttcagcctc
      421 tgccaaagga ggcaaagatc tacaacaacg tcgtctgttc cgtctgcggt gagcaggtgg
      481 cggatggtct tgcacagcag agcgagggaa aatacatctg catcccctgt cagatgcgga
      541 aataactttt acaatttttt tacctgggct gtactttctc cgcagaactg cacagaccat
      601 catgcagaga gattacccca ccgcagccac accgtcactt tggctgttct tcatcatgaa
      661 aattctcaac gcccgcacaa aaagcaaaag aaacctccct tttcaccggc cacacagcaa
      721 catgcaatct ttgaagggtg ccggttttcc cggttcattt gatctacaac cgggaccagg
      781 gatttttctt tttcagatgc tggaacagat catcccacag atcgcacgtg atctcatttc
      841 cgcgggaagt aggagagcag atcccaggcg agggactcgg ccttcttctc gtttgtcatc
      901 atcgtatcca gccggtgagc accgggaaca tcgatctccg tatcgcgttt gtcctgaata
      961 aacatactga cgacatcttt gtacacctcc cacgttccat aggaggtcgg ttcatagccc
     1021 cacgcctgca tcaaagcagc cgccggacca ctgaccggtc tgctgccgat aaagggactt
     1081 accgcaattg taaacttctt tctcaacgct tcccggacac ctgcacactc caaaatagga
     1141 ccgatgcttg ttaccggatt tgacggtccg ataatcacac cctcgctctc ctcaattgct
     1201 gcaatcacct ctggcgttgc tgtgagcggg gcatcacctg cggtttttcg gacaattcct
     1261 gtaatcggca cattgccgcg gcgtcccacc cagtactcct ggtagtgcat caggcttccg
     1321 tcctcggttc ttacgtatgt ggtaacctcc tgatcggaca tcggcagaat cgttgcctgc
     1381 accccgtatg cctttgcaat tttgtccgtc gcttttgtca gagttatccc ctgtctcaga
     1441 aactctgcac gggtaatgtt cgtcgcccga tcgcgatccc caagcggcag gggctcagtg
     1501 tatccgagtt tctccatcgc atgaaatgtc tcaaacgagt cgccgcggat gccccaccag
     1561 ctgtccgtgt tcagcagtcc ggaaaacaga tactgcacgg tatcaatatc gggcgagaca
     1621 tacagccctg acatccagag atcctcggcg gtattgacca ccactgtgat ctcattatcc
     1681 cgcagaattt ggcgaagtcc acggataagt ttcggtgtgc cggttccgcc ggacagtacc
     1741 gttatcatga tactaagtag aagggtgctg catcgtaatc aagatgccgt gttttgaccc
     1801 tgccggtaca ctggcaaaaa atatgatacg gctgctttca gagaaccgaa agcagaccgt
     1861 agatcagaat cgccgcaata cagattggtg cgatgaactt taccatcacc acatacatac
     1921 gttccgcttt aaacggcgac gaactcttca cctcatcaat aaccactttt ggttttacaa
     1981 tccagccgat gaagatacag gtcagaattg ccacaatcgg caggaggata ttgttactga
     2041 agaagtcgaa catatccagc agcgacatac cggcaacaga gatccagtcc agcattccaa
     2101 acccgagcga gatcggaacg gcaagtgcca tcgtgaacaa aaacaccagt aacgatgctg
     2161 tctttcggga cacaccgaac cggtcaccga gcacggctac cggaacctca agcatacaga
     2221 tacaggaggt gagtgctgcg accgccacca gcacaaagaa aattgccgca accgccgagc
     2281 cgaacggcat catagcaaac actttgggca atgcctgaaa catcaggccc ggccccgcgc
     2341 caagcgcctc ggggcttccg ccggaaaaca caaacactgc cggaatgatc aacagacccg
     2401 caagaaacgc aacgccggta tcaaagatct caatggtccg cactgacttt tccatatccg
     2461 ttttctttgc aagatatgac ccgtaggtga tcatcactcc gaatccgagt gtcagcgagt
     2521 aaaacacctg gcccattgcc gcaagcactg tctctgcgga gaacttggaa atgtccggat
     2581 aaatatagta ccacaggccg tccatacctc ccggaaggct gagacagtag gctgtaagtc
     2641 ctataagcat cacgataagt gtcggcatca gaattttgtt tgcccgctca attcccttct
     2701 ccacaccaaa cactacgaca atgcacgcaa gcgccacaaa gagcagtgtc cagaggatcg
     2761 gttcaaccgt gagcgaagta aacccgctga aaaatccgtc cgctgctgcg gtatcggtct
     2821 gccccatgaa gtagaccgca gcatacttca tcacccaccc gccgataagc gagtagtacg
     2881 ggagaatgat cagcggaacc agcagcgaca ggtacccgat gaacgtatat tttcggttca
     2941 gtttggcaaa tgccgttact acacttgtcc cggttgatct gccgactgca atctcggtta
     3001 tcatgatggt aaatccgagc gttaccacga gaatcagata aaccagaaga aaaatgccgc
     3061 cgccgtactc agctgcaaga taggggaacc tccagatatt tccaagacct accgcggccc
     3121 ctgccgctgc aagaatgaat cccatcctgc tggaaaacaa accacgttca ttcatttgca
     3181 tcacaccgat tgaaaaaata aatatttgca gcaggtatct gcagcttagt catcttttgc
     3241 aggagttatc ctgcggcacg cacagcatga ctcattgggg atctcaagcg gatatgctcc
     3301 gcaggaacag gccatgcaca gatcctcact gggaatgccg accgactcca ccagatcctc
     3361 ggttgagatg tactcaaggc ttgttgcatg aatcatatcg cggatttcct cgacagttct
     3421 gccgtttgcg atgagttcct cccgtgtcgg cagatccaca ccgaagtaac agggagcaac
     3481 aatcggtgtt gaaccgatcc gcatatgcac ctcggcagca ccgaactcac gcaccatgtc
     3541 cacaatatgg agcgaggttg tgccgcggac aatgctgtcg tccacgagga taaccgactt
     3601 tccgctgata tggcggcgta ccggattgag tttcatccgt actgcgttct cccgcagact
     3661 ttgggcaggc atgatgaatg ttctgccgac ataccggttc ttcaggagtc cttccatata
     3721 cggaataccg gacgcatccg caaatcccgt tgcaaatgcg gttcctgagt ccggcaccgg
     3781 cgagacaaga tccgccttga ccggatgttt tgccagtttc tcaccgatcc tccggcgtgc
     3841 gtcatacacc agcaccccgt caatgaccga gtctggccgc gcggtataga catactcaaa
     3901 cacacagtat gccttgtgat ccgcagtcag cacctgacga ctggagattc cgttatctgt
     3961 aatgatcacc agttctcccg gtgtcacgtc gcggaggaac tccccggaca gcgtgtcaat
     4021 tgcaacactt tccgatgcta caatgtagcc ggtgtcaagt tttcccagac acaggggttt
     4081 tgttcccagc ggatcacgga acgcatacag cacgccgtcc agaattgcaa cgcccgcgta
     4141 tgctcccttc agtcgtcgaa ttgcattgac aaacgcatcc tctgcgtctg cacctgagat
     4201 gagctcatgg gcaatcattg ccgcaataag ctctgcattt gtagtcgttg aaaagatgtg
     4261 ccctttcccc tcatactctg cacgaagaga ctcgcggttg tcctgcacca gtgcaacact
     4321 cactgttatg gcaagctcat gaccctggaa cgagaacttc agcggctgaa tgttttccgt
     4381 tttaccgcgg tgcgccttct gtgtgtaaag aacctgtcct atgccaacgg ttccaggaag
     4441 tttggataaa atctgttcgt taaaaacttc ggaaagctga ccgggaccct tgtggatcag
     4501 aagagtcttt tcgtgaaaag tagaaatacc tgcagcctcc tggcctctgt gttggagcgc
     4561 atgcagggcg taatataggg ggtaggcgac atcggatgaa tcaacgatgc cggcaatacc
     4621 actcatacaa caaccttttt agttatctgc tgcttttttc gtccatttgt atccgcggat
     4681 cttggagctc tttccaaaac cacatgatga acatacaccg tgccgggcgt ggaatgactg
     4741 ttttccgcag cggcggcaga tgatgtggga gtgcttgttg cgcagaccca ttgatggtgt
     4801 gcctttcgtc attgtttact tcacctcatt tattctacag acggagagat gtagatgaca
     4861 ttgtcaccgc ggacgattaa cgtgccgagc tgaacggtgc cgttctctcc ttcttcctca
     4921 gccttatcaa gaaccaggtt catgtgaacg tcatagccct ggagaacccc gcggatctcc
     4981 cttcctccct tcagtgagat gatcaccggc tggcggttaa ggacctgatc taaaatttca
     5041 agcggtctct ttgtcataag gatacctcgt ttttctccgg tagagtcatt aggacccatg
     5101 gaagagtgct taatgtattc gtctccgaca cacataaaga tagcgtcaga cgaagtgatg
     5161 tgtctctccc gacggcatgg cagataaccg ttcgcatctg aaataactga tgattgcagc
     5221 gtcagatact atgcaaaagt atattgtgat ttttgggaat gttctgctat gtcgtcatat
     5281 gcggatgatg ggatctccga agttgtcgga ttcatctgca ttcttgtact gataacagtt
     5341 gttcttgctg tgtgggccgc agg
//
LOCUS       DALTZZ010000135         1648 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_20300,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000135 DALTZZ010000000
VERSION     DALTZZ010000135.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20300"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="O0X12_02535"
     CDS             <1..408
                     /locus_tag="O0X12_02535"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HJK53070.1"
                     /translation="TVVKDALTAYAAGGVTGMHDATEGGVLGGLYEIAEASGVGMEVW
                     EDKCILPPDVLALCKQFDLDPFGVIAEGCLLITAEADAAPNIIAALTREQIPASVIGT
                     ILADPAVRIMHRSNGQDDVLVVPPEEPFWKIYP"
     gene            complement(463..>1648)
                     /locus_tag="O0X12_02540"
     CDS             complement(463..>1648)
                     /locus_tag="O0X12_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HJK53071.1"
                     /translation="DPAVVEEHIAEFGREDRWLVSRVNSLAAQVTKEMEVCNLHRATR
                     PIATFVLDELSRWYVQLVRPRMWLEEDSVSKKQAYDTMYYVLRRLLTILAPFAPHITE
                     EMYQNLRLSGDVESVHMQDWFVGKADLIDSALEEEMEIVQEFDEAVANARQNGKRKGR
                     WPVGEIVVATSSPKVADAISVMNDMCCDRANARSVKTVTGVWDRVEWTALPVMKAIGK
                     QFGRDGPKVKAFIEESDGTVLKAALARDGKVAMERDGFAAELTEEHMVFEERMPENVF
                     SSPLGEGSTVYVDVSLTPELESEGYAREVIRRIQEMRKQAGLAVDAKISCEVVVMDTR
                     VAELVDRLHDLIADEVRAACLKIRSPEGKTCSCRAVTEPLLKTEWEIDDLKVSISIAQ
                     EN"
BASE COUNT          351 a          582 c          388 g          327 t
ORIGIN      
        1 accgtcgtca aagacgccct caccgcatat gcagcgggcg gtgttaccgg catgcacgac
       61 gcaaccgaag gcggcgttct cggcggcctc tacgagatag ccgaagcatc cggcgtcggc
      121 atggaggtct gggaggacaa atgtatcctg cctccagacg tccttgccct ctgcaaacag
      181 ttcgatctcg acccgttcgg cgtcatcgcc gaaggctgtc tcttgatcac cgcagaagca
      241 gacgccgcgc caaacatcat cgccgcccta accagagaac agatcccggc atccgtcatc
      301 ggaaccatcc ttgccgatcc cgccgtacgg atcatgcacc gcagcaacgg acaagacgac
      361 gtccttgtcg ttccgccgga agaaccgttt tggaagatat atccctgaag tgggactctc
      421 ccttttttct cacgcgaacc gggacatccg gtaaaaaaga agttagtttt cctgagcgat
      481 gctgatcgac actttcagat catcgatctc ccactcggtc ttcagcagag gctctgttac
      541 cgcacggcag gaacaggtct tcccttccgg cgaccggatc ttcagacacg cagcccgtac
      601 ctcatcggcg atcagatcat gcagccggtc aaccagctct gcaacacggg tgtccatcac
      661 cacaacctcg caggagatct tcgcatccac cgcaagacct gcctgtttgc gcatctcctg
      721 aatccggcgg atcacctcgc gggcataccc ctcggactcc agctccggtg taagcgacac
      781 atccacatac accgtggatc cctcgccgag cggtgacgaa aacacattct ccggcatccg
      841 ctcctcaaac accatgtgct cctccgtcag ctccgctgca aaaccgtcac gctccattgc
      901 aacctttccg tcacgggcaa gagccgcctt cagcaccgtt ccgtccgact cctcgatgaa
      961 cgccttcacc ttcggaccat cgcggccgaa ctgcttacca atagccttca tcaccggcag
     1021 agccgtccac tccacccggt cccagacacc cgtcaccgtc ttcacggaac gtgcattcgc
     1081 ccggtcgcag cacatatcat tcatcaccga gatcgcatca gcaacctttg gagaactcgt
     1141 cgccaccacg atctcaccga ccggccagcg tcccttgcgc tttccgttct gccgggcgtt
     1201 tgccaccgcc tcgtcgaact cctgcacaat ctccatctcc tcttccagtg ccgagtcgat
     1261 cagatctgct ttaccgacaa accagtcctg catatgcacc gactcaacat ctccggaaag
     1321 ccggaggttc tgatacatct cctcggtgat gtgcggcgca aacggtgcaa gaatcgtcag
     1381 cagtctgcgc aggacatagt acatcgtatc atatgcctgc ttctttgaca ccgaatcctc
     1441 ctccagccac atccggggcc gcaccagctg cacataccac cgcgacagct catcaagaac
     1501 gaacgttgcg atcggacggg ttgcccggtg aaggttgcag acctccatct ccttggtaac
     1561 ctgtgccgca agcgagttca cgcggctgac cagccagcgg tcctcacggc cgaactctgc
     1621 gatgtgttcc tcaaccactg cggggtcc
//
LOCUS       DALTZZ010000136         2166 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_20870,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000136 DALTZZ010000000
VERSION     DALTZZ010000136.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20870"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="O0X12_02545"
     CDS             <1..330
                     /locus_tag="O0X12_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53072.1"
                     /translation="VQSLRLNQYDDCVLHFTGRDKPWQCYVSDADLYYWYYLLLTPWG
                     KDEHSFFGYMNRAFSTRSESILHVPYWILDYSFKDTLKFTFYLIGHTIGNLICYYTKE
                     ILGKLKR"
     gene            338..1606
                     /locus_tag="O0X12_02550"
     CDS             338..1606
                     /locus_tag="O0X12_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HJK53073.1"
                     /translation="MHITIIGGGYVGLVTAACLSHLNHNTTVIEIFPEKVAAINRAEP
                     PIYEDGLDALLKTHISSNLRATTQYTPVQTSDVIFIAVGTPPNPDGSANLSYITAAAE
                     TIADELAKSHAEYPVIVVKSTVPPGTTKNVVEPIIRRKNPDISFGICMNPEFLREGRA
                     VEDFLHPDRIIVGSSDPKTIRIMHELYTGIAAPIIDVDPTAAEMIKYTSNALLATKIS
                     FANEIGNLCKQLGVNVYEVMAGVGMDSRVSPKFLNAGCGFGGSCFPKDVLALASIARE
                     HGVTPTLLNAVLAVNDSQPLRMVELLETRLGSLSGKRIAVLGLAFKDNTDDVRESRSI
                     PVIEALRRKGAVPVLFDPMAMDTMKAVFPDEEYAPSAAAALTHADGCLVMTEWPEFAK
                     LSSEFDAMRERVIIDGRHILTIDDAEGICW"
     gene            1603..>2166
                     /locus_tag="O0X12_02555"
     CDS             1603..>2166
                     /locus_tag="O0X12_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HJK53074.1"
                     /translation="MSGVKKAVIPAAGWGTRFFPITKAQPKEMVPVVDKPVIQYVVEE
                     AVASGCDDILIITGRNKRAIEDHFDVSAELNEHLRRSGDLALLSESEKLSDLANIHFI
                     RQKEQRGLGDAVLCARQHVGEEPFAVLLGDTICMPDPGEKPCTAQLTDVYLRYHAPVI
                     GVETVPEKKIQDYGIIAGDLVADRVYRI"
BASE COUNT          529 a          473 c          555 g          609 t
ORIGIN      
        1 gtacaatcct tgcggttgaa tcaatatgat gattgtgtcc ttcatttcac tggtagagat
       61 aagccttggc aatgttatgt ttctgatgcg gatctgtatt actggtatta tcttttgtta
      121 accccatggg gtaaagatga gcatagtttt tttggttata tgaatcgtgc cttttctacg
      181 cgttctgaat caattcttca tgttccttac tggattctag attattcttt caaggatact
      241 cttaaattta cattctacct tattggacat accattggta acctgatttg ttattataca
      301 aaagaaatat taggtaaact aaaaagataa tgttataatg catattacca tcattggagg
      361 aggttatgtc ggtcttgtca ctgcagcctg cctctcccac ctcaaccaca atacaacagt
      421 catcgagata ttcccagaga aggttgctgc tattaatcgc gctgaaccgc cgatttatga
      481 agatggattg gacgcacttc tcaaaactca catttcctca aaccttcggg caacaacaca
      541 gtacacccca gtacagacaa gtgatgtcat ctttattgca gttggcaccc cgccaaatcc
      601 ggacggctct gccaacctct cgtacatcac cgctgcagcg gaaacaatcg cagatgaact
      661 tgcaaaatcc catgcggaat atccggtcat tgttgtcaaa agtaccgtcc ctccgggaac
      721 aaccaaaaat gtcgtggaac ccataattcg gagaaaaaat cctgatatct cctttggtat
      781 ctgcatgaat ccggagttcc tgcgggaagg tcgggcagtc gaggatttcc tgcatccgga
      841 cagaatcatt gttggaagct ctgatccaaa aacgatccgg attatgcatg agctctacac
      901 cggtattgct gctccaatta ttgatgtgga tcccaccgct gcggagatga tcaagtacac
      961 ctccaatgcc cttcttgcaa caaagatctc ctttgcaaac gagattggaa acctgtgcaa
     1021 acagctcggt gtaaacgttt atgaggtaat ggcaggtgta ggaatggatt ctcgtgtgtc
     1081 gccaaagttt ctgaatgcgg gatgcggatt tgggggtagc tgcttcccaa aggatgtttt
     1141 ggcacttgca tcaattgcgc gggaacatgg ggtaacaccc acgcttctca atgctgttct
     1201 tgctgtgaat gactcccagc cgttgcggat ggttgaactg cttgagacgc gactcggttc
     1261 cctttcggga aaaaggattg ccgtgcttgg tcttgcgttt aaggacaata ctgatgatgt
     1321 acgcgagtcc cgatccattc cggtgattga agcacttcgc aggaaaggtg ctgtccctgt
     1381 tctttttgat ccaatggcga tggacacgat gaaagctgtt tttccggatg aagagtatgc
     1441 tccctcagct gctgccgccc tcactcatgc ggacggatgt ctcgtgatga cagaatggcc
     1501 ggagtttgca aagctcagtt ctgagtttga tgcaatgagg gaaagggtca tcattgacgg
     1561 gcgtcatatt cttactatag atgatgcaga ggggatttgc tggtgagtgg tgttaaaaaa
     1621 gcggtgattc cggcagctgg ctggggtacg cgtttttttc cgataaccaa ggcgcagccg
     1681 aaggagatgg tgccggttgt tgacaagccg gttatccagt acgtggtaga ggaggctgtc
     1741 gcttccggtt gtgatgatat tctgattata accggtcgga acaaacgtgc gattgaggat
     1801 cattttgatg tttccgcaga gctgaatgag catctgcgcc gcagcggcga tcttgctctt
     1861 ctttccgaga gtgagaaact ttcggatctt gcaaatatcc attttatcag gcagaaggaa
     1921 cagcgcggac ttggtgatgc cgttctttgt gcgagacagc atgtggggga ggagccgttt
     1981 gcggttctct tgggcgatac aatctgcatg ccggatccgg gggaaaagcc gtgtactgcc
     2041 cagctgactg atgtatatct ccggtatcat gctccggtta tcggggttga gacggtgcct
     2101 gagaaaaaga ttcaggacta tggtattatt gccggtgatt tggttgcgga tcgggtgtac
     2161 cggata
//
LOCUS       DALTZZ010000137         2294 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_21199,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000137 DALTZZ010000000
VERSION     DALTZZ010000137.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21199"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            100..1293
                     /gene="ilvA"
                     /locus_tag="O0X12_02560"
     CDS             100..1293
                     /gene="ilvA"
                     /locus_tag="O0X12_02560"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HJK53075.1"
                     /translation="MLTLDRIYHAAYTLRPVIRKTDIIYAPNLSKDAYIYLKTENLQI
                     TGSFKVRGAYYKISRLSPEEKARGVIACSAGNHAQGVALAAAKNGINSLICIPDNAPI
                     SKIEATKSYGAEVELVKGDYDEAYARACELQQKEGRTFIHPFDDDDVIAGQGTIGLEI
                     LDQLPNVDAVIVPIGGGGLIAGIAFAIKQLRPECKVYGVQTEGAPAMKQSFEAHQKMP
                     TSSVSTFADGIAVKCPGDITFDLISKYVDEIVTVTDDEAASAVLELIEHQKLIAEGAG
                     AVAVAAVMFGKLPLNGKNVVCVLSGGNIDVTILSRVITRGLLKSGRVANLTIPLIDKP
                     GELQSISEIIAARGGNVVSVHHDRGEANSAINSCIIKIGMETRDQHQIDEIKAALLDA
                     GYQLI"
     gene            1599..2120
                     /locus_tag="O0X12_02565"
     CDS             1599..2120
                     /locus_tag="O0X12_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53076.1"
                     /translation="MASEKSKGDNNVIKLNGEKKIVIVTSSSKGKGQNGNSKQKGKAA
                     KKAAGSQKKLAKQTKAAKKTLKELKKEKQAAEILMNYCTSMGKYFADQSAIDTLMDVA
                     IDSQNTVDEKAAEIIPDLAAEASVATGETTDEFLKGFHESEEYAAALVAYRNRLETES
                     LILEETDPETPDE"
BASE COUNT          611 a          510 c          653 g          520 t
ORIGIN      
        1 atattaagct tcttttttga aaaaatctac aggaaatccc gctgctgtga ggaatacttt
       61 tttccaaaga ggcagtaatc taacttggga tatcagcaca tgctcaccct tgacagaatt
      121 taccacgcag catatacgct ccggccggtc atcagaaaga ccgatatcat ctatgcgcca
      181 aacctcagca aagatgccta tatttacctg aaaacggaga atctccagat taccgggtcg
      241 ttcaaagtcc ggggagccta ctataagatc tcacggcttt ctccggagga aaaagcgcgc
      301 ggggttatcg cctgctcggc aggaaaccat gcgcagggcg tggcacttgc cgccgcaaaa
      361 aacggaatca actctctgat ctgtattccc gacaacgctc ctatctcaaa gattgaggca
      421 accaagtcgt acggtgccga ggtggaactg gtcaaaggcg actacgatga ggcgtacgcc
      481 cgtgcctgtg agttacagca gaaggaaggc cggacgttta ttcacccgtt cgatgatgat
      541 gatgtgatcg caggtcaggg gacgatcggg ctggagattt tggatcagct gccgaacgtt
      601 gacgcggtga ttgtgccgat cggcggcggc gggttaattg ccggaattgc gtttgccatc
      661 aagcagctgc ggcccgagtg taaggtttac ggggtgcaga ccgagggtgc accggcaatg
      721 aagcagtcct ttgaggcgca tcagaagatg ccgacatctt ccgtctcaac gtttgcggac
      781 ggtattgcgg tgaagtgtcc gggtgacatc acgtttgatc tcatcagcaa atacgtggat
      841 gagattgtaa cggtgaccga tgatgaggcg gcaagtgcgg tgctggagct gatcgagcac
      901 cagaagctga ttgcggaagg agcgggggct gttgcggttg cggcggtgat gtttgggaag
      961 ctgccgctga acgggaagaa tgtggtgtgt gtgctgtccg gcggcaacat cgatgtgacg
     1021 attctgagcc gcgttatcac ccgcggtctg ctgaagtccg ggcgtgttgc aaatctgaca
     1081 attccgctga tcgataagcc cggcgagtta cagagtatct ctgagattat tgcggcacgg
     1141 ggcggaaatg tggtgagtgt ccaccatgac cggggagagg cgaactctgc cattaacagc
     1201 tgtatcatta aaatcggaat ggagacccgc gatcagcatc agatagatga gataaaggca
     1261 gctctccttg atgccggata ccagctcatc tgatactttt tttccggcgg ttctgcgcgg
     1321 gaacaagggt ttggtgatgc tgcggtgcgg aaaggatacg gatatttctc cgctgaaata
     1381 cattctgatt tgaaatccga tagcggtatg cgggaattta cggactgaga caggatacgg
     1441 gcgtatccgt gatgatattg aaaacggcaa aaaaatactc ttgcgataga aaaaatctgc
     1501 aattttttcg ggaaaaagtc cgatccgttc tggtatcacc taaagactaa tatcattaca
     1561 gaatcaacgc agtagtacac aaggagttgt gtactttcat ggcctcagag aaatcaaaag
     1621 gcgacaataa tgtcattaaa ctaaacggcg agaagaaaat tgttattgta acatcctcat
     1681 cgaagggaaa gggccagaac ggcaacagta aacagaaggg aaaggctgca aagaaggcgg
     1741 caggttccca gaagaagctt gcaaagcaga cgaaggctgc aaagaagacg ctcaaggagc
     1801 tcaagaagga aaagcaggcg gcagagattc tgatgaacta ctgtacgtcg atggggaagt
     1861 acttcgcgga tcagagtgcg attgatacgc tgatggatgt tgccatcgat tcgcagaaca
     1921 ctgttgacga gaaggccgca gagatcatcc ccgatcttgc ggcagaggcg agtgttgcaa
     1981 ccggcgagac aaccgacgag ttcctcaaag ggttccacga gtccgaggag tatgcggcgg
     2041 cactggttgc ataccgcaac agactggaga cggagagcct gattcttgag gaaacggatc
     2101 cggagactcc ggacgaataa tattttttta ccagggtaaa tcctgcagat ttgccattca
     2161 ttcttccggc gttttttttt catcatctct tctggcagat acggatcagg gcagacaacg
     2221 gactgttact tcgatgcacg ctttgatata gattcttccc aaaatatttt tgcatgcacg
     2281 aagccctgct gttc
//
LOCUS       DALTZZ010000138         4986 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_21369,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000138 DALTZZ010000000
VERSION     DALTZZ010000138.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21369"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(57..725)
                     /locus_tag="O0X12_02570"
     CDS             complement(57..725)
                     /locus_tag="O0X12_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HJK53077.1"
                     /translation="MTDIVFITDIHGKYEVIPKIFEEEEPDYVLIGGDLTDFGPLDGV
                     IPALEEIPAPTFVIPGNCDPKEIVQVIDASDAISLHKKSIDLGNITITGLGGSNTTPF
                     NTLFELSEEEIAAALSEVLAKTKKNRWNLLVTHAPPQGALDGIGEDGSVHVGSASVAA
                     VVREFDIICCGHIHEQKGITEFERRICVNPGPASEGNYAVITLTDEDEPVIELRNVNE
                     REEA"
     gene            complement(829..2010)
                     /locus_tag="O0X12_02575"
     CDS             complement(829..2010)
                     /locus_tag="O0X12_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK53078.1"
                     /translation="MKNTFVPTKWTLLFLLLAAMLTLMGGAAVAPALPLISKVFSDSP
                     EYLVSMIITLPSLAVACTGYLIGIACDRFGRRPVLVFSLVVFAVAGSAGFYLDSLWAI
                     LVSRIVLGVGIAGLTTATTALITEYYTGASRAKVLGYQSAAMGLGILILETGGGSLAE
                     ISWREPFLIYLVGFVILAGVLLFIHEPSREPRQARHDGTVKLNMGALLPIYASIFVGM
                     AIAFLLPTKLPYLVSEVGTASTTGTGLLLGLMGCCSALAGLFYGRLAARMPRMQVMCL
                     CFFLMGLGCCLLGFAASVTAIAASVIFVGFGQGALIPTIVSWISNKAPAQAMGKATGI
                     FSVALNLGQFGSSLMVVPILAAVGSYSNLFLAGGVCSVLIAVVYAAAWMRERRGTANT
                     V"
     gene            2150..3541
                     /locus_tag="O0X12_02580"
     CDS             2150..3541
                     /locus_tag="O0X12_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HJK53079.1"
                     /translation="MAERENIVTEGSITKALFIIALPIIISNILQSVLEVVDMYFIGH
                     LGDDAIAGGTMSLSIIMVLTTVMFGVVTATAAFISRAYGSKQYERIQVILAHALYLAL
                     IISAIIAVIGLFWSEELLLLMGAEPGALAEGAKFLRPMLMGLFGMIILMTLVTAFQSS
                     GDSRTPMYVMIAVNIVNIILNPTLIQGLAGLPAFGIAGSAYASILSRTAGIVLLLGVM
                     YLHPAFKNSPVRLPVKLTFEPQLVKNIIVIAIPSALQSGIRSFGFLMMTSLITIGYGT
                     VAVAAYGIAIRLDMLGFIIVMGLCTAIAVMVGQNLGAGKVERAEMAVKYAVILNAIFM
                     ACVAVFYYLNAEMLLAFFGAEGETLKDGVIFMHIVPFSYFISAAAMTLGFAMNGAGMT
                     RPGMYSAIAGQICTQVALSAMFAYLGYPFEYVCYAIAFGSVIMCLCDYYFYHKGDWKR
                     KKLNLGGDTAVDG"
     gene            3534..4283
                     /locus_tag="O0X12_02585"
     CDS             3534..4283
                     /locus_tag="O0X12_02585"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53080.1"
                     /translation="MDEELVRRLKKFGMNEYEAKVYAALFGLRVASAREIHEFTQIPR
                     GRVYETLNALMEKHFVLSAGGSPARYHVCDVSRTFDQIKKDTMASLNDLSEYLIELER
                     DRPERLMQAFELRSPWAIDSQVHQMFRRVRSEMLIICDDAEFLKRYGSDLEKLDKRIS
                     LYVIVRTPELAEHLRIRCYLGGKDIEYGMFSPPIPDGRTLLRKIAIYADRQDSLSVVD
                     ENGSLEGVFLSNDFFSGYLYHCILNEIQPIR"
     gene            4421..>4986
                     /locus_tag="O0X12_02590"
     CDS             4421..>4986
                     /locus_tag="O0X12_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53081.1"
                     /translation="MTSPAHLLHSLVLFRNLLRLPVYQKLLALLDDDQSDISRLLDRY
                     AEFVAELYCHTDNLSEYIQKSVYEDDNVYVRLVAEGKTIRPAVAETLQSELRTLEMLS
                     RLTPADLKGDYSGYLPSWQTAKIDLVGGYAERMANIQTTGYGIFAAHHVFIIKNEIIT
                     PLKHPDTQKLSDLYGYEAERRKVIANTKA"
BASE COUNT         1277 a         1311 c         1195 g         1203 t
ORIGIN      
        1 gagaaaaacg cagcaaaata atcttttttg ttcagcaaaa aattccgaag aggggattat
       61 gcttcctctc tctcatttac attccgcagc tcaatcaccg gctcatcctc atcggtcagc
      121 gtgatcactg catagtttcc ttccgatgcc ggacccggat tcacacagat acgccgctca
      181 aactctgtga tacccttctg ctcgtgaata tgtccgcagc agataatgtc aaactcgcgg
      241 acaaccgctg caaccgaggc agaaccaaca tgtacagatc cgtcctcgcc gatgccgtcc
      301 agtgccccct gcggcggtgc gtgtgtcacc agaagattcc accggttctt ctttgtcttt
      361 gccagaacct ctgaaagagc cgctgcaatc tcctcttcag acagctcgaa caacgtatta
      421 aacggcgtcg tgttcgaccc acccaggccg gttattgtga tattcccaag atcaatactt
      481 tttttgtgca gagaaattgc atcggaggca tcgatcacct gcacaatctc ctttggatca
      541 cagttaccgg ggataacaaa ggttggtgcc ggaatctcct caagtgctgg aatcacacca
      601 tcgagcggtc cgaaatccgt caggtctccg cctatcagaa catagtcagg ctcctcctcc
      661 tcgaatattt tggggatcac ctcatacttc ccgtggatat cggtaataaa cacaatatcg
      721 gtcataaata ctatatttcg cggctaagaa tatgaaggtt cagagacctg caaacaaaaa
      781 aactgtacca aaaaagtatg ggaaccctgg gatcagaatc cctgtgtttc agacggtgtt
      841 cgctgtcccg cgtctctccc gcatccatgc cgctgcatac acaaccgcaa tcagtacact
      901 gcagacaccg cctgccagga acagattact gtacgaaccg accgcagcaa gaatcggcac
      961 aaccatgagg gacgatccaa actggccaag gttcagtgcc accgagaaga taccggtagc
     1021 ttttcccatc gcctgagctg gggctttgtt tgagatccag ctcacaatcg tcgggatcag
     1081 tgctccctgt ccgaaaccga caaagatcac ggaagcggcg atcgctgtaa ctgatgcggc
     1141 aaaacccaga agacagcagc ccagtcccat caaaaagaaa cagagacaca tgacctgcat
     1201 ccttggcatc ctggccgcaa gccttccgta gaacaaacct gcgagtgccg aacagcatcc
     1261 catcagtcca agcaggagac cggttccggt cgttgatgct gtaccaacct ctgataccag
     1321 atacggaagt tttgtcggca gcaggaatgc aatcgccatt ccgacaaata ttgatgcgta
     1381 aatcggcagg agtgccccca tattcagttt caccgtaccg tcatgccgtg cctgcctcgg
     1441 ctcacgtgat ggttcgtgga tgaagagcag aacacctgcc aagatcacaa atccaaccag
     1501 atagatcagg aacggttcgc gccaggagat ctctgcaaga cttcctcccc cggtttcaag
     1561 aatcagaatc ccaagaccca ttgcagccga ctgataccca agaacctttg ctctcgacgc
     1621 accggtataa tattcagtga taagtgccgt cgtagcagtc gtcaaacccg caataccaac
     1681 tccgagaaca atgcggctca ccagaattgc ccaaagggaa tccagataga atccggcaga
     1741 tccggcaaca gcaaagacca caagagagaa tacgaggacc ggtcgtctgc cgaaccgatc
     1801 acaggcaata ccgatcagat atcctgtaca tgccactgca agcgacggaa gtgtaatgat
     1861 catcgagacc agatactccg gtgagtcgga gaacaccttg ctgattagcg gcagtgctgg
     1921 agcaaccgct gcacctccca tcaacgtcag catagctgcg agaagcagga acagcagtgt
     1981 ccattttgta gggacaaacg tatttttcat ctctattcat tcatgccaca caccataaat
     2041 aagtggtgca cctgcataca ggagcacttt tagagactca ttgtggaaat ctcccgatag
     2101 aaaaataaca gcactcttat ttcctctcag cccctatata taaacttcaa tggcagagag
     2161 ggagaatatt gttactgagg gcagtataac aaaagccctt ttcatcatcg ctctcccgat
     2221 tattatcagc aacattcttc aaagtgttct tgaagtcgtg gatatgtact tcatcggcca
     2281 ccttggagat gatgcgattg cgggaggaac gatgagtctc tcgatcatca tggtgctcac
     2341 caccgtgatg ttcggagttg ttaccgctac tgctgcattc atatctcggg catatggatc
     2401 gaaacagtac gaaaggattc aggtaattct tgcgcatgcg ttgtatcttg cattaattat
     2461 ctctgcaatc atcgcagtca taggactctt ctggtctgag gaactgcttc tcctcatggg
     2521 agcagaaccc ggggcacttg ctgagggagc aaagttcctg agaccgatgc tgatgggact
     2581 gttcggcatg attattctca tgacgctggt taccgcattt cagagcagcg gtgactcccg
     2641 gacaccaatg tacgtgatga ttgcggtaaa catcgtcaat atcatcctca acccgacact
     2701 catccagggt cttgccggtc ttccggcatt tggtattgcg ggatcagcat atgcatcgat
     2761 tctgtcgcgt acggcgggaa ttgttctgct gcttggagtc atgtacctgc atccggcgtt
     2821 caaaaactcg ccggtccggc tccctgtcaa actgacgttt gaaccgcagc tggtgaaaaa
     2881 catcatcgtc attgcaattc caagtgcact tcagagcggt atcagaagct tcggtttcct
     2941 gatgatgacc tcgctgatta caatcggtta cggaaccgtt gcagttgcgg catacggcat
     3001 tgctatccgg cttgatatgc ttggattcat cattgtaatg ggtctgtgta cagcgattgc
     3061 tgtcatggtg ggtcagaacc ttggtgcagg taaagttgaa cgggcggaga tggctgtaaa
     3121 gtatgcggtc attttgaacg caatcttcat ggcatgtgtt gcggtcttct actacctgaa
     3181 tgctgagatg ctcctggcat tcttcggtgc agaaggcgag acactgaagg acggcgtcat
     3241 ctttatgcac attgtgccgt tctcctactt catcagcgct gctgcaatga cgctcggatt
     3301 tgcaatgaac ggtgccggta tgacgcggcc aggcatgtac tcggcaattg cgggacagat
     3361 ctgtacgcag gttgcacttt ctgcgatgtt tgcgtatctt ggctatccgt tcgagtatgt
     3421 ctgctatgcg attgcctttg gatcggtcat catgtgcttg tgtgactact acttctacca
     3481 caaaggcgac tggaagagga agaagctgaa ccttggtgga gataccgcag tagatggatg
     3541 aggaactggt tcgccgtctg aaaaagttcg gcatgaacga gtatgaggca aaggtgtatg
     3601 cggcactgtt cggcctgcgg gtcgccagtg cccgggagat tcatgagttc acccagattc
     3661 cgcgcgggcg ggtgtatgag acgctgaatg ctttgatgga gaagcatttt gttctgtcag
     3721 cgggaggctc tcccgcccgc taccatgtct gcgatgtgtc gcggacattt gatcagatca
     3781 aaaaggacac aatggcctca ctcaatgatc tgagtgagta cctgatcgaa ctggaacgtg
     3841 accgccctga acggctgatg caggcctttg aactgcggag tccgtgggca attgattctc
     3901 aggtacacca gatgttccgg cgtgtccgtt cagagatgct gatcatctgc gatgatgcgg
     3961 agttcttgaa acgttatgga tccgatctgg aaaagctcga caaacgaatt tctttgtatg
     4021 tgatcgtcag aacaccagaa cttgcagagc atctaagaat ccgctgctat ctcggcggca
     4081 aagacatcga gtacggaatg ttctccccac cgatcccgga cggcagaact cttctgagga
     4141 aaattgccat ctatgccgat cggcaggatt ccctgtctgt ggtagacgag aacgggtcat
     4201 tggaaggcgt gttcctctcc aatgattttt tctcagggta tctgtatcac tgtatcctca
     4261 atgaaattca gccgattcgc taacacgtga ccttttttca gggcagattc atcaacgata
     4321 ccgtttcctg tagcagaaaa aagaacgaaa tccctgtttt caaattccgc caaaacctat
     4381 ttcctctctc acctggaaac ataaccagca cataccagcc atgacatctc ccgctcatct
     4441 tctccacagt cttgtcctgt tccgcaacct cttgcgtctg cccgtttatc agaaactcct
     4501 tgcccttctt gatgacgacc aaagtgatat ctcacggctc cttgacaggt atgcagagtt
     4561 tgttgccgaa ctctattgcc acacagacaa tctcagtgag tacatccaga aaagtgtcta
     4621 tgaggacgac aacgtctatg tcaggcttgt tgctgaagga aaaaccatcc ggccggcagt
     4681 tgcagaaacc cttcagtcag aacttcggac actggagatg ctcagccgtc ttactcccgc
     4741 agacctgaaa ggagattaca gcggatacct tccttcctgg cagaccgcaa agatcgatct
     4801 tgtcggcggg tatgcggaac gaatggcgaa cattcagaca accggctacg gaatttttgc
     4861 agcccatcat gtctttatca tcaaaaacga gatcatcacc cccttaaaac accctgacac
     4921 gcagaaactt tccgaccttt atggatatga ggctgagcgg aggaaggtca ttgcaaacac
     4981 gaaagc
//
LOCUS       DALTZZ010000139         3536 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_21429,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000139 DALTZZ010000000
VERSION     DALTZZ010000139.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21429"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="O0X12_02595"
     CDS             <1..1416
                     /locus_tag="O0X12_02595"
                     /inference="COORDINATES: protein motif:HMM:NF036590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HJK53082.1"
                     /translation="LDLYHRKFAGRNASPNDWLETMGSIGKIDFSTMAGRWLTQTGYP
                     TVTATASYNPEEQTAEITVEQTGFGDQDPWIFPLVGTLLTDKGNTVAEIFEKIDGAHQ
                     TFSVPCAGAFAAASWNPGHAAYIRINSTASDDELYLLLKYDTDTVSRFLAWQTLVDRE
                     MYKLCSNPAAVTDPRLVNWYIETLTNNTKMQSTGVLPLTIFEYTSDPAYSYCYTTLHA
                     AKQRILRSVSETLGKTRLNVLYTTYNLPERQTTTPAELARIFKARAVKNLILTHLAAF
                     DTPDVHEILKTACQTATCATDRTHALTLYLSSSAPDRLDLLKTELTRAKADPVAWENF
                     LAAVAASSSPDTVRYLRYIEQSGNLHIEQAGESRSLYLRFAANRKISLETEEGREFLS
                     DSLTTLARVNEYISTGILNVFSHLDSYEDTVRDACFAILTSLINTAPETEAPSVIRTA
                     RRILQKSPKAKEAYDQAHRST"
     gene            1743..2159
                     /locus_tag="O0X12_02600"
     CDS             1743..2159
                     /locus_tag="O0X12_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HJK53083.1"
                     /translation="MKQMIANGMTDTEVIAKFKEENCWGLCTSIDLKDCDPAAIRDAD
                     KIHQFVLELADVIEMHRFGEPQIIHFGASDRVAGYSMTQLIETSLLSAHFVNETNSAY
                     IDIFSCKEYPPSVAAEFSKKFFGAKDMNTTVFFRTI"
     gene            2223..2711
                     /locus_tag="O0X12_02605"
     CDS             2223..2711
                     /locus_tag="O0X12_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53084.1"
                     /translation="MVPGLSLHLAKGYLSTMTEVMRMIHTYRPMRARSRRTRRVSVFS
                     NVYRLPDGRIMYIWYNKNPITGTARPYIAADDGTTHQAYIPPLPDQIRQKYPALNPSD
                     TRMAAAMQIKLAKLLRLPKTDPEPTAEERLSQLADWLAVMYRLTDTVPTAAEQQPVSY
                     PN"
     gene            2874..3143
                     /gene="gatC"
                     /locus_tag="O0X12_02610"
     CDS             2874..3143
                     /gene="gatC"
                     /locus_tag="O0X12_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HJK53085.1"
                     /translation="MVSENDVLHIAELADIGIATSELGKFTEQFNAILEYFDILDTLA
                     AEDELDRPLVNVFREDEVRPSLTQEEALANSHNPEEGYIRAPKVI"
     gene            3144..>3536
                     /locus_tag="O0X12_02615"
     CDS             3144..>3536
                     /locus_tag="O0X12_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HJK53086.1"
                     /translation="MTDPYNAFLSDIEIPSSAAGPLAGVRVAVKDNISTKDIPTTCAS
                     KILLGYIPPYDAHAVELLKAAGATIAGKTNMDEFGMGTTTENSAFGPALNPRDTTRVT
                     GGSSGGSAAAVAAGLVPMALGSDTGGSIR"
BASE COUNT          973 a         1045 c          758 g          760 t
ORIGIN      
        1 cttgatctgt atcaccgtaa gtttgcgggc aggaatgcat ccccgaacga ctggcttgaa
       61 accatggggt ccatcggaaa aattgacttc tccaccatgg caggccgctg gctcacacag
      121 accggctacc caaccgtcac cgcaacggcc tcatacaacc ccgaggaaca aactgccgag
      181 attacagttg aacagaccgg attcggggat caggatccct ggatcttccc tctcgtagga
      241 accctgctca ccgacaaagg aaacaccgtt gcagagatct ttgaaaaaat cgacggggcg
      301 caccagacat tttctgtccc gtgtgcagga gcttttgctg ctgcatcctg gaaccccggc
      361 cacgctgcct acatccgcat caacagcacc gcatcagacg acgaactgta cctcctgctc
      421 aagtacgata cagataccgt cagcagattc cttgcatggc agacactggt agatcgtgag
      481 atgtacaaac tctgcagcaa tcctgcggca gtcactgatc cgcgtcttgt caactggtac
      541 attgagacat tgaccaataa taccaaaatg caaagtaccg gtgttcttcc cctgaccatc
      601 ttcgagtaca caagcgatcc cgcctactcc tactgttata ccaccctcca cgcagcaaaa
      661 cagcgtatcc tacgatcggt gtccgaaacc ctgggaaaga cacgcctcaa tgtactctac
      721 accacctaca acctcccgga acgtcaaacc acaacaccgg cggaacttgc acgtatcttc
      781 aaagcacggg cagtgaagaa tctgatcctt acccatctcg cagcctttga cacacctgat
      841 gttcacgaga ttctcaaaac agcctgccaa accgcgacct gcgctacaga tcggacgcat
      901 gccctcacgc tgtacctctc aagttccgca cctgacaggt tggatctcct gaagacagaa
      961 ctcacccggg caaaagccga cccggttgcc tgggagaact tccttgcagc cgttgccgcg
     1021 tcctcctcgc cagatacagt acgctatctc agatacatcg aacagagtgg aaacctccac
     1081 attgaacagg caggagaaag cagatccctc tacctccggt ttgctgcaaa ccgcaagatc
     1141 tcccttgaaa cagaagaggg acgcgagttt ctttcagact ctctcaccac ccttgcccgg
     1201 gttaacgagt acatctcaac cggcattctc aacgtcttct cccatcttga cagctatgaa
     1261 gacaccgtcc gtgatgcgtg ttttgccatt cttaccagcc tgataaacac cgctcccgag
     1321 accgaggctc cgtccgtcat ccgtaccgca cgccgcattc ttcagaaaag tccgaaagcc
     1381 aaagaagcat atgatcaggc gcaccggagt acgtaagcag cagaacgccg catatggctt
     1441 gcccgtataa atatgaaagg gcgcagcgtc cctgaaaacc gtattttttt attcacgtca
     1501 caccctgcca attttgattt tgaatgatat actcatttaa aattgaaatt tattagcaaa
     1561 taggcagcag aattgaaatt taagcaaata aagtttgtag aaatcatttt taacaatttt
     1621 acaaggatat tttcagcagg tatctttaaa tatactgggg cagacctatt attatccaaa
     1681 gcatcccggc aggaaactgt gtgggccgga actttgagaa gtatggagaa tagccctgat
     1741 ctatgaaaca gatgatagca aatggcatga ctgacaccga ggtcattgca aagtttaagg
     1801 aagagaactg ctgggggctc tgtacctcaa tcgatctcaa ggactgtgat ccggcagcga
     1861 ttcgcgatgc agacaaaatc catcagtttg tccttgagct cgcagatgtt atcgagatgc
     1921 accggttcgg cgagccccag ataattcatt tcggcgccag tgaccgcgtt gccggctact
     1981 caatgaccca gttaatcgaa acctctcttc tctctgcaca cttcgtaaac gaaaccaact
     2041 ccgcatacat cgacatcttc agctgcaaag agtatccgcc aagtgttgcc gcagagttca
     2101 gcaagaaatt cttcggtgca aaagacatga ataccacagt attcttccgc acaatctgat
     2161 tttcacggga gaatcttttt cttttttgta caactgttac aggggaagca ctttcagacc
     2221 gcatggtccc cggtttatcc cttcaccttg caaaagggta tctgagtact atgacagagg
     2281 tgatgcgcat gatacacaca tatcgcccaa tgcgggcccg ctcccgcaga accagaagag
     2341 tgtccgtctt cagcaatgtt taccgtctcc ccgacggcag aatcatgtat atctggtaca
     2401 acaaaaaccc gatcacagga acggcacgtc cctacattgc agcggatgac gggacaacac
     2461 accaggcata catacctccg ctgccggatc agattcggca gaaatatcct gctctgaacc
     2521 cttcagacac ccgcatggcc gcagcaatgc agatcaagct tgccaaactt ctcagactgc
     2581 caaagacgga tccagaacca accgccgagg aacgactatc ccaacttgcc gactggctag
     2641 ctgttatgta ccgtctcacg gatacagtac ccacagcagc cgaacagcag ccagtcagct
     2701 atccgaacta aagaccacat ccgcactata ataatacacc ccaactgctc tgcgccggac
     2761 caaggatcac cggaactttt tccaaaaaac ggatcgatcc tgccggaaga gtagtaactc
     2821 tttttaccca aaacaacatc atcactaaac tactattcac aggtcgcaga aatatggtct
     2881 ctgaaaacga tgttctccat atcgcagaac ttgccgatat cggtattgca acgtccgaac
     2941 tcggcaaatt caccgaacag ttcaatgcaa ttcttgaata cttcgatatc ctcgacaccc
     3001 ttgccgcaga agacgaactt gaccgcccgc tggtcaatgt attccgcgaa gatgaggtca
     3061 gaccctctct tacacaggag gaggcccttg caaactcgca caatcccgaa gagggataca
     3121 tccgcgctcc caaggtgatc taaatgaccg acccgtacaa cgcattcctc tcagacatcg
     3181 agatcccctc ctcagccgcg ggaccacttg ccggtgtccg cgtcgctgtc aaagacaaca
     3241 tctccaccaa agatatcccc actacctgcg cctctaaaat ccttctcggc tacatcccgc
     3301 catatgatgc ccatgcagtt gaactgctca aggcggcagg ggcgaccatc gccggaaaaa
     3361 ccaatatgga cgagttcggt atgggaacca ccacggaaaa cagtgctttc ggtccagcat
     3421 taaacccccg cgacacaaca agagtcaccg gaggatcctc tggaggttcc gcagccgcag
     3481 tggccgcagg tcttgttccg atggcacttg gatcagatac cggcggatca atccgc
//
LOCUS       DALTZZ010000140         2224 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_21460,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000140 DALTZZ010000000
VERSION     DALTZZ010000140.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21460"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="O0X12_02620"
     CDS             <1..683
                     /locus_tag="O0X12_02620"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HJK53087.1"
                     /translation="AAIAGTLTPKFSNWSAKGDLTQIPNILTKSITYGLLLAVPTAVG
                     GILLSDRLLYFFYGAEFAAGGTACAILLLLQIVNVFMVFLGTTLSAIDHARQSFYASG
                     SAALLNVALNVLLIPIIGIEGAAVASLVSITLNAVLLRYYLGQYLTVRCDLRSAGHIL
                     LSAFLMTAVILVYMQFVPLTNIFLVLVPVAIGAVLYFVVLFRLNREIHDEIADLAKQF
                     GLPWPHWL"
     gene            complement(688..1626)
                     /locus_tag="O0X12_02625"
     CDS             complement(688..1626)
                     /locus_tag="O0X12_02625"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJK53088.1"
                     /translation="MKKYTWDDPRLNEEARAFLKTRPENLGDVWERIVRDAVSGDTAE
                     LFRVRNRRSAETPAPEGVEIADADANGVPVRLYIPVSKNRPKKIILYFHGGGWVIGSI
                     QSCSRFCGELAKAADVIVAAAAYRLAPEFPYPAAVNDCREAYLWIQRHAAEYGGDDGC
                     IYAAGDSAGGNLAIVLALQERLCGLMLFYPVTTVVPGSYGESWEEFSHGYALDAKLME
                     AFSDAYVPEILRDNPLVSPLESADLSRLPQTLCITAECDILRDQGRRFCDLLRKAGVS
                     VTELCFPGAVHLFVTVAGMDGDFRKGVFECTAFLQK"
     gene            1625..>2224
                     /locus_tag="O0X12_02630"
     CDS             1625..>2224
                     /locus_tag="O0X12_02630"
                     /inference="COORDINATES: protein motif:HMM:NF017515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJK53089.1"
                     /translation="MIFFIYLQFYNVAIIMTSCQDRNRDSIPKVIHHTWFGEDPYPKL
                     VRKCIASWKKHLPDYDIITWTPDNFDMDICPWVRESYEAKKYAYTADYVRYYVLHNYG
                     GIYLDADVEVKKNFDPLLNSAGFIAFLHDHLTGVVLGSVKGLSYFGDVIDSYNNTSFL
                     KGAVSEVEFLESTLIRDGLLLNGKFQEINGFCVYPYTYFP"
BASE COUNT          521 a          620 c          484 g          599 t
ORIGIN      
        1 ctgccgcaat agccggaacc ctcaccccca agttcagcaa ctggagtgca aaaggcgatc
       61 tcactcagat tccgaacatc ctgacaaaaa gtatcaccta cggacttctg cttgccgtac
      121 cgactgccgt aggcgggatc cttctgtctg accgtctgct ctacttcttc tacggtgcag
      181 agttcgccgc tggcggaacc gcctgtgcca tcctgcttct gctccagatc gtaaacgtgt
      241 tcatggtctt ccttggtaca accctcagcg ccattgacca tgcccgtcag tccttctatg
      301 caagcggctc tgccgcactc ctcaacgttg ccctcaatgt actgctcatc ccaatcatcg
      361 gtattgaagg ggctgcggtt gcgtctcttg tcagcatcac cctcaacgct gtccttctca
      421 gatactacct gggccagtat cttactgtcc gctgcgacct ccgctccgca ggccatatcc
      481 tccttagtgc cttcctgatg acggcggtta tcctcgtcta catgcagttc gttccgctca
      541 ccaacatctt ccttgttctt gttccggttg cgatcggtgc tgtcctctac tttgtcgtcc
      601 tgttcagact aaaccgggag attcacgacg agatcgctga ccttgcaaaa cagttcgggc
      661 ttccctggcc gcactggctg taatgagtta tttctgtaaa aatgcggtgc actcaaaaac
      721 acctttccgg aaatctccgt ccataccggc aacagttaca aacagatgta ctgctccggg
      781 aaaacagagt tccgtaactg atactccggc tttccggagc aggtcacaga acctcctccc
      841 ctgatcccgc agaatatcac actctgcagt aatacacagc gtctgcggta gccgggaaag
      901 atccgcggac tcaagcggcg acacaagcgg attatcccgc agaatctctg gaacgtatgc
      961 atcggagaat gcctccatca gttttgcatc cagggcatat ccgtgagaaa actcctccca
     1021 cgactcaccg taacttccgg gtacaaccgt cgtcaccgga taaaagagca tcagaccgca
     1081 caaccgctcc tgcagcgcca ggacaatcgc aaggtttcct cccgcactgt ctcccgcggc
     1141 atagatacat ccgtcatccc cgccgtactc tgcagcatgt ctctgtatcc agagatatgc
     1201 ctcccggcag tcatttactg cggcaggata cggaaactca ggcgcaagcc ggtatgcagc
     1261 cgcagcgaca atcacatccg cagcttttgc aagttcaccg cagaaccgtg aacagctctg
     1321 gatgctcccg ataacccagc cgccgccgtg aaaatagagt atgatctttt ttggtctgtt
     1381 tttggataca ggtatataca accgcacggg aaccccgttg gcatctgcat ccgcaatctc
     1441 aaccccttcc ggtgcggggg tctcagcaga cctcctgttt cgaacgcgga aaagttctgc
     1501 agtatcaccg ctgacggcat cccgcacaat tctctcccac acatccccaa gattttccgg
     1561 tcgcgtcttt aggaacgctc ttgcctcctc attcaaccgt ggatcatccc aggtgtattt
     1621 tttcatgata ttttttattt atttgcagtt ctataatgtt gctataatta tgacctcctg
     1681 ccaagatcga aatcgggatt cgataccaaa agtaattcat catacgtggt ttggagagga
     1741 tccttatccc aaacttgtac ggaaatgtat tgctagttgg aaaaagcatc tgcctgatta
     1801 tgatataatc acatggacac ctgataattt tgacatggat atctgcccat gggttcgtga
     1861 atcatatgag gcaaaaaaat atgcgtacac tgcagactat gttcgctact atgtactcca
     1921 taattatggt ggaatatatt tggatgcgga tgtggaagtc aaaaagaatt ttgacccgct
     1981 gttaaattca gctgggttta ttgcatttct acatgatcac cttacgggag tcgtgttggg
     2041 atctgtcaaa ggtttatcct attttgggga tgtaatcgac tcctataaca atacatcgtt
     2101 tttgaagggg gctgtttccg aggttgagtt tcttgaatcc actttgattc gcgatggact
     2161 tttactaaat ggtaaatttc aggaaataaa tggtttttgt gtgtatcctt atacctattt
     2221 tcca
//
LOCUS       DALTZZ010000141         2468 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_21544,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000141 DALTZZ010000000
VERSION     DALTZZ010000141.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21544"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="O0X12_02635"
     CDS             complement(<1..436)
                     /locus_tag="O0X12_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53090.1"
                     /translation="MDEIVEQGFDRLQTLISETKQKQDDAYRELCNQSAALLSRMIEA
                     VAPIAGEIGSEFLLKAKQDTKGELYDQKYYDEKMIILGKTDSPMEYRPDDPKKKVTQQ
                     FCVLSEKGVLYELMFSNDGFVVDTFASPLTPEDALVFYGYDIM"
     gene            518..1447
                     /locus_tag="O0X12_02640"
     CDS             518..1447
                     /locus_tag="O0X12_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HJK53091.1"
                     /translation="MDYFIALNQILILFLLIGVGFFCRRVGILNETSVASLSKFLLHI
                     CIPAMIIYSMQIPFTSELLQNGELLILAAFGYYAVSLIVAWFAPILLRAKHEEYGVFR
                     FMLVFSNSMFMGFPVLSMIFGSEAIFYAAIFNIPFTILAYSLGIWLLTVHGGGEGRPV
                     FQPKLLLNAAFLSTFVGLALFLTSTTIPDPINGSLELLDSVTTPLSLVVTGGFLAQLD
                     LSRIFGNVRQYFVAGIRLLILPALVYVIFSPFIEDPLILGVVVVTAGMPAATNTVMLA
                     SEHRVHPDIAAQGVFITTLVSVFTLPLLAMFLS"
     gene            complement(1561..1644)
                     /locus_tag="O0X12_02645"
     tRNA            complement(1561..1644)
                     /locus_tag="O0X12_02645"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1610..1612),aa:Ser,seq:gga)
     gene            complement(1678..>2468)
                     /locus_tag="O0X12_02650"
     CDS             complement(1678..>2468)
                     /locus_tag="O0X12_02650"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HJK53092.1"
                     /translation="VGSMLKSSVGHSGTLDPMVSGVLVVMLGKAVRLAPLLLLHEKEY
                     VACLRLHADVPRERVEEAVKQFTGRIYQRPPRRSAVKRALRIRVIHRIELLDMQDRLV
                     LIQVRCEAGTYIRSLCVHIGRILGCGSQMIELRRTRSGGFSVEDTHTLHEIRDAVERG
                     DEDLIRSMLLPPEKAITDIPKIVVTPKAADAVAHGAVLAGVGVLQCDPFVQKQNVAIV
                     TEEGKLIALAEAVRDSAKFVCGEPGFVARSTRVFVSPNDVNKKY"
BASE COUNT          561 a          625 c          608 g          674 t
ORIGIN      
        1 acataatatc atagccgtag aacacaagtg catcttccgg agtaagtggt gatgcaaagg
       61 tgtcaacaac gaatccgtca ttggagaaca tgagttcata taaaactcct ttttcagaga
      121 ggacgcagaa ctgctgtgta acttttttct tcggatcatc cggccggtac tccatcgggg
      181 agtcggtttt tccaagaatg atcatctttt cgtcgtagta tttctgatcg tagagttccc
      241 ctttggtatc ctgttttgct ttgagcagga actctgatcc aatctcacct gcgatcggtg
      301 caacagcctc aatcatccgc gagagaagtg ctgcgctctg gttacagagt tcacgatatg
      361 cgtcatcctg cttctgtttt gtctcgctaa tgagtgtctg gaggcggtca aatccctgtt
      421 cgacgatttc gtccatacca taggtatgga cgggtccgat agtttaacta tcggttcggt
      481 tttatattgt aaataatagt aaaggaggcc atagactgtg gattatttta tcgccttaaa
      541 ccaaatttta attctgttcc tccttatcgg ggtgggattt ttctgcaggc gtgttgggat
      601 tttgaatgag acgtcagttg catccctttc gaagtttttg ctgcatatct gtattccggc
      661 aatgatcatc tactcgatgc agattccgtt tacatcagaa ctcttgcaga acggtgagct
      721 gttgatcctt gcggcatttg ggtattatgc ggtttcactg attgttgcat ggtttgcacc
      781 cattctcctg cgtgcaaaac acgaggagta cggagtattc cggttcatgc tggtgttttc
      841 gaactcgatg tttatgggat tcccggttct gtcgatgatc ttcggatcag aggcaatatt
      901 ttatgcggcg atctttaaca ttccgtttac gattctggca tactcactag gtatctggct
      961 cttgaccgtt cacggaggag gagaaggacg cccagtgttt cagccgaaac tgctgttgaa
     1021 tgcggcattc ctctcaacgt tcgtgggtct tgcactgttt ctgacatcaa caacgatccc
     1081 ggatccgatt aacggatctc tggagctgct ggatagcgtg acgacaccgc tgtccctggt
     1141 tgttacggga ggttttcttg cacagctgga tctttcgcgg atctttggaa atgtacggca
     1201 gtattttgtc gcggggatcc ggctgctgat tctgccggcc ctggtatatg tgatcttctc
     1261 gccgttcatt gaagatccgc tgatcctggg tgtggtggtg gtgacagcag gtatgccggc
     1321 ggcaacaaac accgtgatgc ttgcatcaga acatcgtgtg catccggata ttgcggcaca
     1381 gggcgtgttt atcaccacgc tggtgagtgt atttaccctg ccgcttcttg cgatgttcct
     1441 gtcgtgaaac acagccgcaa ttcatttcca cttttttaga gaacaaaact cctgttcagt
     1501 acgatactta tcggaaaacg gatccaactc tatacagaaa ttacacaaga aaaacaaaag
     1561 cgctgagggg gagatttgaa ctcccgagag actttcgcct cacgggcttt ccaggcccgc
     1621 gccctaccgc tagactacct cagcaaacta gcatattacg tgcgttgtct gtgtttatta
     1681 atatttcttg tttacatcat tgggcgagac aaaaacccgg gtagatcgtg ccacaaatcc
     1741 cggctctccg cagacaaact ttgccgagtc acgtactgcc tctgcaagcg caatcagttt
     1801 tccctcttcg gtaacgattg caacattttg tttctgtaca aacggatcac actggagtac
     1861 gcctacaccc gcgagaacgg cgccgtgtgc aacggcatcc gctgctttgg gcgtgacaac
     1921 aatcttggga atatctgtga tggccttctc aggggggagc agcatggaac gaatcagatc
     1981 ttcatcaccc cgctccacgg cgtcacgaat ctcatgcaga gtgtgtgtgt cctccaccga
     2041 aaatccgccg gaacgtgtac gccgcaactc gatcatctgc gaaccacacc caagaatccg
     2101 gcctatgtgg acgcacaacg atcgtatgta ggttcctgcc tcacagcgaa cctggattag
     2161 gactagccga tcctgcatat ccagaagctc gattcggtga attacccgga tgcgcagtgc
     2221 ccgcttcacc gccgaacgcc gcggcgggcg ctgataaatg cggcccgtga actgctttac
     2281 agcttcttca acacgttcgc ggggaacatc ggcatggaga cggagacatg ccacgtactc
     2341 tttctcatgc agaagcagta agggtgcaag ccgcacggct tttccgagca taaccaccag
     2401 cacacctgag accatcgggt caagggtccc cgaatgtccc acactacttt tgagcatact
     2461 gccaaccc
//
LOCUS       DALTZZ010000142         4711 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_21615,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000142 DALTZZ010000000
VERSION     DALTZZ010000142.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4711)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4711)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4711
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21615"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="O0X12_02655"
     CDS             complement(<1..1052)
                     /locus_tag="O0X12_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HJK53093.1"
                     /translation="MDFDDLIISPDGTHHLYEGTPIYEHRFQSVGPFCFPGIAAVCDN
                     TGAYHINLLGEPAYDERYVWAGDFAEGVAPVRNKEGRYLFIDEAGKPIAFETYLYATG
                     FSEGSAVVYHETLGATHITTAGELLYGDWYYDARPFAGGRAYVRDDDGWMFITQDGSA
                     LERTEEPNMPFPLRGTVRYVPPKNPIPETLRSSEWDAAAVLMRHGEREPFIKGQPGST
                     KVLTARGKRQSRAFGGALPKVPIRTYASPMIRCVQTGDQILVGAGVSGETQKSLMLGT
                     PSAYVADDDIVKEFYVVNPVKIMSLRYVAGETLPGHYPVKEGTERMFSFVESTLKDGE
                     ISVCVTHDAWIVPFVSV"
     gene            complement(1062..2087)
                     /locus_tag="O0X12_02660"
     CDS             complement(1062..2087)
                     /locus_tag="O0X12_02660"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HJK53094.1"
                     /translation="MIKVAVNGHGTIGKRVADAVSAQPDMEIIGVSKTKPGAEAFVAK
                     ERGYPLYIADITKKEAFEKAGIPVAGSVEEMIQKADIVVDATPGGVGVGNKALYEKYG
                     KKAIWQGGEDHEVAGFSFNASCNYKDAIGRQYARVVSCNTTGLCRIINLIDSAFGVKK
                     VRATMVRRGGDPGDIKRGPIDAIVLNPVTIPSHHGPDVQSVLPHINIVTSAMIVPTTL
                     MHMHFLNMELKNPATKEQVIELIKAHSRLGLIEKSTGITSTAQLKEYTSDLLRPRGDL
                     WENGIFKDSISVLDGTDLYLFQAIHQEADVVVETVDCIRAMAGDVTSAAESIAITNKA
                     VGLTAIR"
     gene            complement(2727..4355)
                     /gene="putP"
                     /locus_tag="O0X12_02665"
     CDS             complement(2727..4355)
                     /gene="putP"
                     /locus_tag="O0X12_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HJK53095.1"
                     /translation="MYLYPAARCGAGGRNTTAGGIFVLDWNLIGIGAAFIIYFAAMIY
                     IGFFYFKKTKTTSDYVLGGRKLHPFVAAMSAEASDMSGWLLLGLPGLAYATGMSAAGW
                     TAIGLAIGTYLNWKFIAKRLRIYTHKAKDSLTIPEFITNRFQEKKGFITAIASIFILI
                     FFIVYTSSQFKAGGVLFSSLMTIYGVDLTSIGITDASSLYIAGVLICALIVVCYTFTG
                     GFKAVCITDTIQGMLMLFALILVPITVCIILFGEGVSFSSIDPQMFSLFHEYNAASGI
                     FEFVSVFTIVSGLAWGFGYFGQPHILPRFMAIENPEEIPKARMAAMIWVILSLAAAIF
                     VGLVGQLLFPNPAVVGDSEQIFIQMTGSVFGAVPFIAGIIYCGILGAIMSTASSQLLV
                     ASSAISQDLYKNLINKEAPDKRLLWISRVTVLIVSVVAIALSLDQASSVFNIVSNAWA
                     GFGCTFGTVILLGLFWKRMNWQGALAGIIVGGIVSLGWGTYLTAVTNLYEMVPGVIAS
                     LLAIYLVSRFTAEPEKEVTDMYDEYVAAVGDRST"
     gene            4511..>4711
                     /locus_tag="O0X12_02670"
     CDS             4511..>4711
                     /locus_tag="O0X12_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HJK53096.1"
                     /translation="MCEDTNCLCGAFEDECGCEGFYHEMPRLGEPAPDFVAETTHGQI
                     KLSDYKDKKWVVLFSHPADFTPV"
BASE COUNT         1162 a         1207 c         1145 g         1197 t
ORIGIN      
        1 acagagacga acggcacaat ccatgcatcg tgggtaacac acacggatat ttccccgtct
       61 ttaagggtac tttccacaaa agaaaacatt cgttccgttc cctctttaac aggataatga
      121 ccggggaggg tttcaccggc aacgtaacgg aggctcatga tcttcaccgg atttactacg
      181 tagaactcct tgacaatgtc atcatctgcg acgtaggcac tgggtgttcc aagcatcaga
      241 cttttttgtg tctctccgct gacacctgcc ccgaccagaa tctgatctcc ggtctgcacg
      301 caccgtatca tcggactggc atatgtccgg ataggtacct tgggaagtgc tccaccaaaa
      361 gcacgcgact gccgttttcc ccgggccgta aggacttttg tggatcccgg ctggcctttg
      421 atgaacggtt cgcgttctcc gtgccgcatc agaacagctg ccgcatccca ctctgaactc
      481 cgcaatgttt ccggaatagg atttttcggc ggaacatacc gtaccgttcc ccgcagagga
      541 aacggcatgt tcggctcttc tgttcgttcc agagccgaac cgtcctgcgt gatgaacatc
      601 catccatcat cgtcgcggac atatgccctc cctcctgcaa acggtcgggc atcgtagtac
      661 cagtctccat acaacagttc tcctgcggta gtgatgtggg ttgcaccgag cgtctcatgg
      721 tagaccaccg ctgatccttc cgaaaatccg gtcgcataca gatacgtctc aaaggcgatc
      781 ggttttcctg cttcatcaat gaacaggtat ctcccttctt tgttgcgaac gggagcaaca
      841 ccctcggcaa agtctcctgc ccatacgtac cgttcatcgt atgcaggttc accgaggagg
      901 tttatgtgat aggctcccgt gttatcgcag accgcagcaa tcccgggaaa acagaacggg
      961 ccgacgctct gaaaacggtg ctcgtagatc ggtgtcccct cgtaaaggtg atgtgttccg
     1021 tccgggctaa tgatcagatc gtcaaagtcc ataaaaaaag attagcggat tgcagtaaga
     1081 ccaaccgctt tgttggtgat cgcaatcgat tcggctgcag acgttacatc gcctgccatc
     1141 gcccggatac agtccaccgt ctcaaccacg acgtctgctt cctggtggat tgcctggaag
     1201 agatagagat cggttccgtc aagcaccgag attgagtcct tgaagatacc gttttcccaa
     1261 aggtctccgc gtggacgaag gagatcagac gtgtactcct tgagctgggc tgtactcgtg
     1321 ataccggtac tcttctcaat taaaccgagg cgggagtgtg ctttgataag ctcgatcacc
     1381 tgctctttgg ttgcgggatt tttcagttcc atgttgagga agtgcatgtg catcagagtt
     1441 gtcggcacaa tcattgctga ggtaacgata ttgatgtgcg ggagaacact ctgtacatca
     1501 gggccgtggt gcgaagggat tgtgaccgga tttaagacga tggcatcgat aggtccgcgt
     1561 ttgatatctc ccggatctcc tccgcggcgg accatggttg cacgaacttt tttcacgcca
     1621 aaagcagagt cgataagatt gatgatgcgg caaagaccgg ttgtgttgca ggagacaacg
     1681 cgggcatact gtctgccgat tgcgtctttg tagttacagg atgcgttgaa ggaaaatccg
     1741 gccacctcat gatcctcgcc accctgccag atcgcttttt tgccgtactt ttcgtagagt
     1801 gccttgttcc cgactccgac accaccgggt gttgcatcga cgacgatgtc agctttctga
     1861 atcatctcct caacacttcc agcaacagga attcccgctt tttcaaaggc ctcttttttg
     1921 gtgatgtctg ctatgtaaag cggatatccc cgttcctttg caacgaacgc ttctgcaccg
     1981 ggttttgttt tggagacacc aatgatctcc atgtcgggct gtgcggagac tgcatcggcc
     2041 acacgttttc cgatggttcc atggccgttg actgcgacct tgatcataat acagtatgct
     2101 ttctcttcgg tatcataaaa gcatatccag taatccattc gagagcagca gatcggtttt
     2161 cttcttcttg gaaaaatcct tagagtaatt tttccatctg cgcgatgctt tccttctact
     2221 gtactttttg ctgtttccat gaagatccaa gcatcatttt tctttgatgc atcgcagcat
     2281 tgtatggtcg cgggaaggaa acctagtttg tgctcaagag gtttattagt atcatcattc
     2341 agattattac attgtaatat ttcgatggtc gtgcatcacc ggtacagtga aaaagtacat
     2401 ccggcacctg aaggaagtat catttttcct gtctcggtgc ggcacgtatg ttgttggcat
     2461 ttcgacccat cggatattac tctaaaacca tcgcggtcat aatgataccc ggtatcgagg
     2521 ccgcggagaa cttcagcctt gtacctaact ccccgacctg gtgcagaaat gcagtggcca
     2581 ggttggctga aggacacgtt ttggatctgt tttacatgtt ttttgcagat gtgtacaccg
     2641 tattcgcatt gccacatctt tttcactaat gggtgtcttt gtcaaaagcg attgaagaga
     2701 taatctgaaa aaagaaacgg gagtttttag gtactccggt caccaactgc tgctacgtac
     2761 tcatcgtaca tgtcggtgac ttccttctcc ggctcggcgg tgaaccgcga gacaaggtag
     2821 attgcaagca gacttgcaat tacacctggc accatctcat acagattcgt gaccgcggtc
     2881 aggtaagtac cccagccaag agagacgata cctcccacga tgattccggc aagtgcaccc
     2941 tgccagttca tccgtttcca gaagagacct aaaaggatca ctgtaccgaa cgtacaaccg
     3001 aatcctgccc atgcgtttga tacaatattg aacactgagc ttgcctgatc cagtgagagg
     3061 gcaattgcaa ctactgagac gatcagaacc gttacacggg agatccagag cagccgcttg
     3121 tcaggagctt ctttgttgat caggttcttg tagagatcct gtgagattgc cgatgatgcc
     3181 acaagaagct gggatgatgc ggtactcatg atcgcaccga ggataccaca gtagatgata
     3241 cctgcgatga atggaactgc accaaacaca cttccggtca tctgaataaa gatctgttca
     3301 gagtcaccga caactgccgg gttcgggaac agaagctgtc ccacaagacc tacaaagatt
     3361 gcagcggcaa gagagagaat cacccagatc attgctgcca tacgtgcctt gggtatctct
     3421 tcggggttct cgattgccat gaaccgtggg agaatgtgcg gctgaccgaa gtagccgaat
     3481 ccccaggcaa ggccggaaac gatcgtaaac actgatacga actcaaagat accgcttgct
     3541 gcattgtact cgtggaacag tgagaacatc tgcggatcga tcgaggagaa gcttaccccc
     3601 tcaccgaaca gaatgataca aacggtgatc ggtacaagaa tcagggcaaa cagcatcagc
     3661 attccctgaa tggtgtcggt gatacagact gctttgaaac ctccggtaaa ggtgtagcag
     3721 accacaatca gtgcacagat caatacccct gcaatgtaga gcgaggatgc gtccgtgatt
     3781 ccgatggagg ttaaatcaac accataaatc gtcatcagag agctgaacag cacacctccc
     3841 gccttgaact gtgatgaggt atagacgatg aagaatatca gaataaagat agatgcaatt
     3901 gcggtgatga accctttctt ttcctggaaa cggttcgtga taaactcggg aatcgttagt
     3961 gagtcctttg ctttgtgcgt gtaaattctc aggcgctttg caatgaactt ccagttcaga
     4021 taggttccga tggcaagacc gattgcggtc cagccggctg ccgacatacc ggttgcgtac
     4081 gcaagacccg gcagaccaag gagcagccat ccgctcatat cggatgcttc ggcggacatt
     4141 gctgcaacaa acggatgcag tttacgtcct ccaagcacgt agtcacttgt ggttttggtc
     4201 ttcttgaagt agaagaaccc gatatagatc attgcagcga agtagatgat gaacgccgct
     4261 ccaatgccaa ttaagttcca gtctaacaca aatattcccc cggcagtggt atttctcccg
     4321 cctgccccac accgggctgc cgggtacaaa tacacaaagt tactgcaact tacttaattc
     4381 gttaacattc tatttatgtg ctcttacaca gacttcgaaa cggtttttcc gatccgaatc
     4441 cgtcattttg ccgttaactg aacacttatt ccttcatgga ttcaacagac atacaaggtg
     4501 aaccacaaaa atgtgtgaag ataccaactg tctttgcggc gcattcgagg atgagtgcgg
     4561 ctgcgaaggt ttctaccatg agatgccccg tctcggagaa ccggctcctg attttgttgc
     4621 agagactact cacggccaga tcaaactctc agactataag gataagaagt gggtcgtgct
     4681 gttctcccat ccggcagact ttaccccggt c
//
LOCUS       DALTZZ010000143         2642 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_21659,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000143 DALTZZ010000000
VERSION     DALTZZ010000143.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21659"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="O0X12_02675"
     CDS             <1..589
                     /locus_tag="O0X12_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HJK53097.1"
                     /translation="TGAADAVAAMIREGYIDVLFAGNALATHDLEYSIFGTSLGMDLR
                     SGELVSGGHRHHLYTINKIMDAGSIYAAVQTGIVKSGIMYECIKNNIPFVLAGSIRDD
                     GPLPDVIQNVIEAQDAMRRHIPELGMVLMVATLLHSVAVGNLLPSHVKTICVDINPAS
                     VTKLMDRGTSQAIGMVSDAGTFMPLLLAELRELSA"
     gene            complement(631..1608)
                     /locus_tag="O0X12_02680"
     CDS             complement(631..1608)
                     /locus_tag="O0X12_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine biosynthesis radical SAM protein
                     RaSEA"
                     /protein_id="HJK53098.1"
                     /translation="MSAPIKPLASWTGQDRYFGEVLPSLTGIFLSGGCSWNRCRMCGY
                     KNDRNTCGSRQDLIDHMTAQIAWLAENYASDTYGLAKIFTSGSVFDPVEVPREVLDGF
                     GSLFAGKPVIAESRAEYVTEDALSRFLSTLDQGQSHPLTVAMGLETTNDAIREKSIDK
                     GFSFADFIRASEICHDAGVGVKAYLMMKPLFLTEQEAMEDMQKSIAEAAPYADMISMN
                     LCTVQGRTELEQYWQRGSFRPPYLWSALKVLLDAEVSVACDPVGGGFRRGPHNCGICD
                     KEIVAAINEYSLTADKSVLQAVWDAGCSCRKEWEFVLDNETSWNMPLTE"
     gene            1770..>2642
                     /locus_tag="O0X12_02685"
     CDS             1770..>2642
                     /locus_tag="O0X12_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53099.1"
                     /translation="MNNGTRKTRILLLASGLLVLLRCAGTVSAYSSDFVTPDSQTATL
                     KMGVGSPIVVECVTDNIPGGATVRIVLAKSGGSVVESKSVVIQSDGRFGATFDTWDLA
                     GGMYTVTVQEPKDYPFGSKRNWFSVQLVDRTGELTSTSPRVQYSTDEVLVAGKASETG
                     SRGVELTMYEGTTTDGRVVYGPTWVGTNENGQFSETLPATGAGVYQVVVKDKDGYIGL
                     LTYTLMSGSATSTSTTTATPTSSGMTLTANAEASHDSPAYFAVTTGTGTATITAFRGV
                     DWTVEYTVNNGAVVK"
BASE COUNT          686 a          759 c          661 g          536 t
ORIGIN      
        1 caccggagcc gccgatgcag tcgccgcaat gatccgcgaa ggatacatcg acgtactctt
       61 cgccggaaac gcacttgcaa cccacgacct tgagtacagc atcttcggca cctccctcgg
      121 catggatctc cgcagcggtg agctggtcag cggcggtcac cgccaccacc tctacaccat
      181 caacaagatc atggacgcag gttcaatcta cgccgcggtt cagacaggga ttgtaaaaag
      241 cggtatcatg tacgagtgca tcaaaaacaa catcccgttc gtcctggccg gatccatccg
      301 tgacgacgga cctctgccgg acgtaatcca gaacgtcatc gaggcacagg acgccatgcg
      361 cagacatatc cccgaactcg gcatggtctt gatggttgca acacttttgc actccgtagc
      421 cgtcggaaac ctcctcccgt cccatgtaaa aaccatctgc gtggacatca accccgcatc
      481 ggtcaccaaa ctgatggacc gcggaacctc ccaggcaatc ggtatggtca gtgatgcagg
      541 aacgttcatg ccgctgctgc ttgcagaact gcgggaactt tccgcataat cttttttcaa
      601 aacagaagtt acagaaaaac cgcgaaaaaa ttactcggtt aagggcatgt tccacgacgt
      661 ttcattgtca agaacaaact cccactcctt cctgcaggaa cagcccgcat cccagaccgc
      721 ctgaagaacg ctcttgtcag cagtgagcga gtactcattg atcgcagcaa cgatctcttt
      781 gtcacagata ccgcagttgt gcggaccccg gcggaacccg ccgccaaccg gatcacaggc
      841 aacactgacc tcggcatcca gcagaacctt cagggcagac cacagatacg gcgggcggaa
      901 cgaaccccgc tgccagtact gctcaagctc ggttctcccc tgcaccgtac acagattcat
      961 cgagatcata tccgcatagg gggcagcttc cgcaattgac ttctgcatat cctccattgc
     1021 ctcctgttcc gtcaaaaaca gcggtttcat catcagatac gccttcacgc cgacacccgc
     1081 atcatgacag atctcggatg cacgaatgaa atccgcaaag gaaaaaccct tgtcgatgga
     1141 cttctcacgg atagcatcat tcgtcgtctc aagacccatt gcaacggtca gcggatgaga
     1201 ctgcccctga tccagagtgg acaaaaaccg tgaaagggca tcctcggtca cgtactcggc
     1261 gcgcgactcg gcaatcaccg gttttccggc aaagagactg ccaaatccgt ccagaacctc
     1321 gcggggaacc tcgactggat caaatacact gcctgaggtg aatattttgg caagcccgta
     1381 cgtatcggag gcgtagttct ccgcaagcca ggcaatctgg gcggtcatat gatcgattag
     1441 gtcctggcgc gaaccgcagg tgttgcgatc atttttgtaa ccgcacatcc tgcagcggtt
     1501 ccagctgcat cccccggaaa gaaaaatgcc ggttaatgac ggcagaacct cgccaaaata
     1561 tctgtcctgt cccgtccagg aggcaagagg ttttattggt gctgacatga ctcttatctc
     1621 atggtattgc aggcattatc ttagtttccc agaacacagg atggagtcca gaaaaatccg
     1681 ggaggagatg cagaaacgac ctggtttcag gataaaacag agaacgcgga caacaaagcc
     1741 tatctatata gaaaacaact atttagatta tgaataacgg tacgagaaaa acaaggattc
     1801 tccttctcgc gtccggtctg ctggttctgc tgcgttgcgc cggaaccgta tcggcgtact
     1861 catcagattt tgtcacgccg gacagccaga ccgccacgct caaaatggga gtgggatctc
     1921 ccattgttgt tgaatgtgta accgacaata tcccaggagg tgccaccgta cgtattgttc
     1981 tggcaaaatc aggcggtagt gtcgttgaat caaaatcagt ggttattcag tcagacggcc
     2041 gcttcggagc aacctttgat acctgggatc ttgccggagg tatgtataca gtaaccgttc
     2101 aggaaccgaa agattacccg tttggaagca aacgtaactg gttcagcgta cagctggttg
     2161 acagaaccgg tgaactgacc agcacctcac ccagagttca gtactccacc gacgaggtac
     2221 tggtcgcagg caaagcaagt gagaccggat cacgcggtgt ggaactgacg atgtatgagg
     2281 gaaccaccac cgacggtcgt gtagtatacg gccctacctg ggttgggaca aacgagaacg
     2341 gccagttctc tgagacactc ccggcgacag gagccggtgt ctatcaggtt gttgtcaaag
     2401 acaaagacgg ctacattgga ttactgacct acacgctgat gagcggaagt gcaacctcca
     2461 cctcgaccac aactgcaacc ccgacctcct caggaatgac gctgaccgca aatgcagagg
     2521 catcccatga cagcccggca tactttgcgg taacgaccgg aacagggacc gcaaccatca
     2581 ccgcattccg cggcgttgac tggacggtcg agtataccgt taacaacggt gcagttgtca
     2641 ag
//
LOCUS       DALTZZ010000144         2850 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_21721,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000144 DALTZZ010000000
VERSION     DALTZZ010000144.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21721"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(235..1863)
                     /gene="arcS"
                     /locus_tag="O0X12_02690"
     CDS             complement(235..1863)
                     /gene="arcS"
                     /locus_tag="O0X12_02690"
                     /EC_number="2.6.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002948 - archaeosine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002927 - archaeosine-tRNA biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine synthase subunit alpha"
                     /protein_id="HJK53100.1"
                     /translation="MSTFEVRGRDGLARLGLLRLEETEIQTPAAVEMQKLFPSLEDRA
                     FTNLPLSAPASDAERYYVPGSNPVLFHPQAACPAVSGDVVMVSGWRQALGDAKRAAEY
                     LGAVLAGTPPDTARYAPAAALPSNAAMLIATGFDLFDYTAVDLASVQGKFCLPEGEFD
                     ASYMEKGVCGCEGCRTQNLFLHNRLALDREIAFVKTWISAGQMRELMEQRCRLNAEQV
                     ALLRRLDRSAFAAAALPVVRSSRFLANSAESMTRQEIAFFEDRVVNRFVPSRNDVCVL
                     LPCAARKPYSLSKTHQKFQRVIEGRAHEVIITSPLGVVPRELELVYPAGHYDVPVTGY
                     WDREESAILVGYVAAYLAKHRYDRVICHLEGGAKAVAVAAAEAAGVTLEFTCTDDRPT
                     SPESLRALNAALKDCRKRRADPIRGTLAWQFGEIVDTKGWITKGRYPNQKVYEKKTQM
                     FSIDPLTGLMRPTMDGWRLLSGYRVRISEGFVPQGDILAPGVVWADPAIREGDEVLVE
                     GPGYLATGKAAMSADEMVRSSRGVAVRVRKTRKS"
     gene            complement(1860..>2850)
                     /gene="tgtA"
                     /locus_tag="O0X12_02695"
     CDS             complement(1860..>2850)
                     /gene="tgtA"
                     /locus_tag="O0X12_02695"
                     /EC_number="2.4.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(15) transglycosylase TgtA"
                     /protein_id="HJK53101.1"
                     /translation="EIFGPDAPLSGPVQGAVFEDLRETAGRTVSEMGFAYCPVGAVVP
                     LMEAYRYRELVDVIIAAKKGLNPGACVHLFGAGHPSMFALAVALGCDVFDSAAYALYA
                     KEGRYITPSGTLKLDEMSELPCACPVCRSHTADELRKSPDRQKLLAYHNLAVTMAEIS
                     RVRAAVQEGTLWELVDERCRAHPRLLDGYRRLLERSGELEHLDRATKRRFFYRGSESC
                     ERTEVRRYHEMIPRVKLSEVSLIDAGGPVPSRFSEVIGFKPPFGPVPYELAETFPVGP
                     CEIPSWDETMVSYGIRGLAALFAANPQTKVTISTTEQWAPMFREAFPECEVIT"
BASE COUNT          663 a          912 c          738 g          537 t
ORIGIN      
        1 accctacctc tcgtgaaaaa gacgctgaag cacctaaaat gccggaaata aataacagaa
       61 tcaaaacgga ttatctgcgt aacgagcacc ggatccgtca gaactccccg aaaatcggga
      121 tccaatactc gttgctataa tatatgtttc aatcctataa agcagtacag atagtactac
      181 attttcttcc caaaaaaata aaaaaaataa tcccggtgct ccggcacaaa ccgtttaact
      241 ctttcttgtt tttcgtaccc ggaccgcaac accgcgggat gatctgacca tctcatctgc
      301 actcatcgcc gcctttccgg ttgcaagata ccccggcccc tcaaccagca cctcgtcacc
      361 ctcccggatg gcaggatccg cccacacaac acccggcgca agaatgtcgc cctgcggtac
      421 aaaaccctcg ctgatccgca cgcggtagcc ggagaggaga cgccacccat ccatcgtcgg
      481 ccgcatgagg ccggtcaaag gatcgatcga gaacatctgg gtcttcttct cgtacacctt
      541 ttgatttgga taccttccct ttgtaatcca tccctttgtg tcaacgatct cgccgaactg
      601 ccaggcaagt gttccgcgga tcggatcggc ccggcgtttg cggcagtcct tcagggctgc
      661 attcaatgca cgcagcgact caggagaggt gggacgatca tccgtgcagg taaactccag
      721 tgtgactccc gcagcctcgg cagccgcaac cgcaaccgct tttgctccgc cctccagatg
      781 acagatgacc cggtcatacc ggtgtttggc aaggtacgca gcaacatacc cgaccagaat
      841 cgccgactcc tccctatccc agtaccccgt taccggcaca tcataatggc ccgccggata
      901 caccagctcc agctcccggg gaacaacgcc aagaggcgac gtgataatga cctcatgggc
      961 tcttccctcg ataacccgct ggaacttctg atgcgtcttg gaaagggagt acggttttcg
     1021 tgcagcacac ggcagcagca cacacacatc gtttcgcgac ggaacaaaac ggttcaccac
     1081 acggtcctcg aagaatgcaa tctcctgacg cgtcatcgac tcggccgagt ttgccaaaaa
     1141 ccgcgacgac cgcacaaccg gcagtgccgc agcagcaaac gccgaccgat cgagtctccg
     1201 gagaagagca acctgctccg cattcagtct gcaccgctgt tccatcagtt cccgcatctg
     1261 ccctgccgaa atccaggtct tcacaaatgc gatctcccga tcaagtgcaa gccggttgtg
     1321 gaggaaaagg ttctgcgttc tgcatccttc acagccgcag acgcctttct ccatgtacga
     1381 ggcatcaaac tccccttccg gaagacagaa ctttccctga accgatgcaa gatccaccgc
     1441 cgtgtaatca aacagatcaa aaccggttgc aatcagcatc gcagcattcg acggaagggc
     1501 ggctgccgga gcgtatcgtg cggtatccgg cggtgttccg gcaagaaccg caccaagata
     1561 ctctgcggcc cgctttgcat cccccagagc ctgccgccat ccggagacca tcaccacatc
     1621 gccggagacc gcgggacatg ccgcctgcgg atgaaacaga accggattgg atcccggtac
     1681 ataataccgc tccgcatccg atgcgggggc ggagagcgga aggtttgtga acgcccggtc
     1741 ctccagcgaa ggaaagagct tttgcatctc caccgccgca ggagtctgaa tctccgtctc
     1801 ttcaagacga agcagaccca gacgggcaag tccgtcccgg ccgcggacct caaacgtact
     1861 catgtgatga cctcacactc cgggaacgcc tcccggaaca tgggtgccca ctgctctgtc
     1921 gttgagatcg tgacttttgt ctgggggttt gccgcaaaaa gtgctgcaag gccccggata
     1981 ccgtaggaga ccatcgtctc gtcccaggag ggaatctcgc acggacctac cgggaacgtc
     2041 tccgcaagct catacggaac cgggccgaac ggcggtttga acccgatgac ctcagaaaac
     2101 cgtgacggaa ccggtccgcc tgcatcgatc agcgaaacct cggagagttt tacgcggggg
     2161 atcatctcat ggtaccgccg cacctcggtc cgttcgcagg actcggatcc gcggtagaag
     2221 aaccggcgtt tcgttgcacg gtcgagatgt tccagttcac cggaccgttc cagcagacgg
     2281 cggtaaccgt ccagcagccg cggatgagcc cggcaccgct catccaccag ttcccagagg
     2341 gttccttcct gcaccgctgc acggacccgg gatatctcgg ccatcgtaac cgcaagattg
     2401 tggtaggcga gcagcttctg gcggtcgggg gatttgcgga gttcatccgc cgtgtgcgac
     2461 cggcagaccg gacaggcaca gggaagttcg ctcatctcat ccagcttcag cgttcctgac
     2521 ggcgtgatgt agcgtccttc ctttgcatac agtgcatacg ccgccgagtc aaagacatca
     2581 caaccgagag caaccgcgag tgcaaacatc gacggatggc ctgcaccgaa cagatggaca
     2641 caggctcccg gattgagacc tttctttgcc gcgataatga catccacaag ttcgcggtac
     2701 cggtatgcct ccatcaaagg gacaacggca cccaccgggc agtaggcaaa acccatctcg
     2761 gagacggtcc ggcctgcggt ttcccggaga tcttcaaaga ctgctccctg cacggggccg
     2821 gataggggtg catcggggcc gaagatctcc
//
LOCUS       DALTZZ010000145         1749 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_21777,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000145 DALTZZ010000000
VERSION     DALTZZ010000145.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21777"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(6..1319)
                     /locus_tag="O0X12_02700"
     CDS             complement(6..1319)
                     /locus_tag="O0X12_02700"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJK53102.1"
                     /translation="MIIVIGGGPAGRMVAVRLASAGRKVTLLERRALGGQCVHDGCML
                     ICGLNDVARSIRNTEFLRGCGVIDGSVSVRYPEVVRNLEKVQARLSQILERETLDAGV
                     KIEYGVTAEVRDGKVIVDGSVREAEAVVIACGAEMNIPDVPGAGLPGTYTARTIRSMP
                     ELPKRMAIIGGGITAAEFAYIFSAFGVEVLLFARHKLLSILPDRMQKAVRRDLSSVEI
                     YEDRTLDAVLGEESVCGVVAGGETFACDAVLFATGMRPSSPHVSGVLKREDGSIVVNE
                     RMETSVPGVYACGDVTGAPYFTPVSRLQGFAAADAILGNPRTVDLSRIPFTVVLGLDY
                     TVCPCADESGATFSSPNIAGPGSVWHVSDGSVGSMELTVVPDSGKIVGFSSSGPGTGI
                     VGTYLGYLVRKGVTVHEFSPMLEVHPISDGLYSMIRFAKDHLGND"
     gene            complement(1320..>1749)
                     /locus_tag="O0X12_02705"
     CDS             complement(1320..>1749)
                     /locus_tag="O0X12_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53103.1"
                     /translation="YTSRGEANYKGKMVSFDDEGSGWVNAINGLETDFGDAAPVLGSI
                     PTDADVMSPVNTKAETEERVRADLVQKLTKRVRIKTTSGDAIFAEEKEFRPSPDNIEI
                     TVEKVYVPVWQVRGKKDIVEVNAYTGEELSLPSDEGCEVF"
BASE COUNT          475 a          555 c          341 g          378 t
ORIGIN      
        1 gaaactcagt cattcccgag atgatccttt gcaaaccgta tcatcgaata caagccatct
       61 gaaattgggt gaacctcaag cataggggaa aactcatgca ccgttactcc ttttcgcacc
      121 aagtatccaa gatacgttcc cacaatacct gtacccggcc cagaactgga aaaacccaca
      181 atctttccgc tatccggtac aacagtaagc tccattgatc ccacagaccc gtctgaaaca
      241 tgccacacag atcctgggcc tgcaatattc ggagaggaaa atgttgcacc ggattcatca
      301 gcacacgggc aaaccgtgta atccaacccg agcacaacgg taaacggaat acgggacaga
      361 tccaccgtcc gcggattgcc aagaattgca tcagctgcag caaacccttg caaccgtgac
      421 accggtgtaa agtacggagc accggttaca tcaccgcagg catacacacc gggaaccgac
      481 gtttccatac gctcattcac cacaatagat ccatcttccc gtttcagaac accagataca
      541 tggggactgg acggacgcat tcccgttgca aacaacacgg catcacacgc aaacgtctca
      601 ccccctgcaa caactccgca aaccgattcc tcacccaata ctgcatcaag tgtacgatcc
      661 tcgtaaattt caaccgatga caggtcacgc cgtacagctt tctgcatccg atcgggaagg
      721 attgacaaca gtttgtgccg ggcgaaaagc agaacctcaa caccaaatgc tgaaaagata
      781 taggcaaact ctgcagccgt aatacctcca ccaatgattg ccatacgttt cgggagctct
      841 ggcattgatc gaattgtccg ggcggtataa gtccccggca accctgcacc tggaacatcc
      901 ggaatattca tctctgcacc gcatgcaata accaccgcct cagcctcacg tacagatcca
      961 tccacaatca ctttaccgtc gcgaacctcg gccgttactc cgtactcgat cttcactccg
     1021 gcatcaagcg tctcacgttc aagaatctgg gataaccgtg cctgaacttt ctcaagattg
     1081 cgcaccactt cgggatacct gacagagacc gaaccgtcaa tcactccaca gccgcgtaag
     1141 aattcagtat ttcggatcga tctggcgaca tcattcaacc cacagatcag catacacccg
     1201 tcgtgcacac actgccctcc tagtgcccgc cgttccaaaa gagtaacctt tcgaccggca
     1261 gaggcaagac gcacagctac catcctgccc gccggacctc caccgataac gataatcatt
     1321 taaaacactt cacatccttc atccgacggc agtgataact cctcgccggt gtacgcattc
     1381 acctcaacga tatctttctt gccgcggacc tgccacacag gaacatacac tttctcaaca
     1441 gtaatctcaa tattatccgg tgacggacgg aactcctttt cttccgcaaa aattgcatcg
     1501 ccggaggttg tcttgatccg cacgcgtttt gtcagtttct ggacaagatc agcgcggaca
     1561 cgctcctcgg tttccgcctt agtatttacc gggctcatta cgtctgcgtc tgtaggtatc
     1621 gaacccaaaa ccggtgctgc atcaccaaaa tcagtctcaa gaccattgat tgcattcacc
     1681 catccagacc cctcatcatc aaagctcacc atctttccct tgtagtttgc ctcaccacgg
     1741 cttgtgtac
//
LOCUS       DALTZZ010000146         1681 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_21805,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000146 DALTZZ010000000
VERSION     DALTZZ010000146.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21805"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="O0X12_02710"
     CDS             <1..563
                     /locus_tag="O0X12_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53104.1"
                     /translation="PYCGGTPAPYDTKEKIYATLHTREGTQKITVFVRRYHCRDCGRL
                     LYADEPFYPDTRLGSTIVDLATALSLLNSFSHTAEIINAMGIEIDRGSVRKYALSDLP
                     VPKINFFYGFPLPFSLFSLIGRPLGLKSGAAAGAEVLAASGFPSAYRAPPDGTGTSRA
                     RQEQRKEQKQKEKREAQKPADRSKQE"
     gene            complement(596..1519)
                     /locus_tag="O0X12_02715"
     CDS             complement(596..1519)
                     /locus_tag="O0X12_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H4MPT-linked C1 transfer pathway protein"
                     /protein_id="HJK53105.1"
                     /translation="MIGIDVGGANLKVVDEEGVHIHYCPLWRESNLAEILAEYKGADA
                     AVVMSGELADGFFNKAEGISYIVGAVQRTFPNARFYGTDGRFHSSACPELAAANWLAS
                     VDYLRRSYPAGMMLDIGSTTADIVPFARFDELRGLTDTLRLQRGYLVYTGMLRTPVAT
                     LASSAVINGKVTPFSTEYFACSGDAHYVLGNITEAEYTAATPDGKEVSREACLRRLAR
                     TVCADLEEIGEDGAVAVAQAFWDAQRTLVCSAVEKAAADAQTREILVGGIGSPVFAPL
                     VGGTDLTAAVGIPADALPAFAVKELAEAGAE"
BASE COUNT          379 a          553 c          430 g          319 t
ORIGIN      
        1 gcccctactg tggcggaact cccgcaccct acgacacaaa agaaaagatc tacgcaaccc
       61 ttcacacccg tgaaggaacg caaaagatca cggtatttgt gcggcggtac cactgccgcg
      121 actgtggaag actgctgtat gccgatgagc ccttttatcc tgataccaga cttggctcaa
      181 cgattgtgga ccttgcaaca gctctctcgc ttctcaactc cttttcccac accgccgaga
      241 tcatcaacgc catgggaatt gagattgacc ggggttcggt gagaaaatat gcgctctccg
      301 accttccggt tccaaaaatc aacttcttct acggattccc gctgccgttc tcactcttct
      361 cccttatcgg ccgacccctg ggtctcaagt cgggcgccgc agccggggca gaagttctcg
      421 cggcctctgg tttcccatcc gcataccggg caccgcctga cgggacggga acctcccgtg
      481 cccgccagga acagcggaaa gagcagaaac aaaaagaaaa acgggaagcc caaaagccag
      541 cagaccgcag taagcaggag tgaccctgca agaagggcaa ccgtccagga aagcttcatt
      601 cagccccggc ttccgcaagt tccttcaccg caaacgccgg aagcgcatca gcaggaatac
      661 caaccgccgc ggtgagatcc gttcccccga ccagcggggc gaaaacgggc gatccgattc
      721 cgcccacaag aatctcccgg gtctgtgcat cagccgcggc cttctccacc gcactgcaca
      781 caagcgttct ctgtgcatcc cagaacgcct gggcaacggc cacggctccg tcctcgccga
      841 tctcctccag atccgcacag accgtccgtg ccagccgccg gaggcacgcc tccctgctca
      901 cctcttttcc gtccggcgtt gctgcggtgt actcggcctc ggtgatgttc ccaagaacat
      961 agtgggcatc gccgctgcat gcaaagtact cggtggaaaa cggcgttacc tttccgttga
     1021 tcaccgcaga acttgcaagc gttgcaaccg gcgtccggag catgcccgta tagacaagat
     1081 atccccgctg aagtctgagc gtatccgtca gcccccgcag ctcatcgaac cttgcaaaag
     1141 gcacaatatc tgcggtggtg ctgccgatat caagcatcat tcccgcagga tacgaccggc
     1201 gcagataatc aacggacgca agccagttcg ccgcagcaag ctctgggcag gctgaggaat
     1261 ggaacctccc gtcggttccg tagaaccggg cattggggaa tgtcctctgc acagcaccga
     1321 caatgtagga aatcccctcg gctttgttga aaaaaccgtc ggcaagctcg ccgctcatga
     1381 cgaccgcggc atccgctccc ttatactccg caagtatctc ggcaaggttt gactcgcgcc
     1441 agagcgggca gtaatggatg tgaactccct cctcgtccac cactttgagg tttgccccgc
     1501 cgacgtcgat gccgatcatg aaaaggtaac tccgccgacc tcgtcgaaca ccgctttttt
     1561 gccgttgtag tgaacaccct ccggcggaag tccgacggtt gccttgagaa gaacatcggc
     1621 tatctcctcg tcaatgacac cgacaacacc gatgatgctc atcgtcggac ggggattgac
     1681 a
//
LOCUS       DALTZZ010000147         2268 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_21870,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000147 DALTZZ010000000
VERSION     DALTZZ010000147.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21870"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            23..553
                     /locus_tag="O0X12_02720"
     CDS             23..553
                     /locus_tag="O0X12_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53106.1"
                     /translation="MTLGSSGDSPNKKEENPTSPSETSSRFKQIICPGCFEEMAKEEL
                     IGNKCPLCGYRLSPEEMDDEILGSGDEELTWMLTQNLQRTLLSWLMDLGAAPLAAYRI
                     ASRVCEQENIPVKSGRTTAFSFTARMTPEEKAAEKQCKVCGRTFTTGGRKIVSGDLFE
                     PEPSVEYFCDDCIPQR"
     gene            688..1047
                     /locus_tag="O0X12_02725"
     CDS             688..1047
                     /locus_tag="O0X12_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HJK53107.1"
                     /translation="MGDFLGDLKKGINKSVEDLNKSVKTLEIQSKISSLKKEADAVYA
                     EIGKRAVQENGLEKYGELGDKLQVLLTDIKAEEEKLPAEASDVKKTKICPGCSTENPV
                     DARFCSCCGEMLEDQSP"
     gene            complement(1035..1826)
                     /gene="surE"
                     /locus_tag="O0X12_02730"
     CDS             complement(1035..1826)
                     /gene="surE"
                     /locus_tag="O0X12_02730"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HJK53108.1"
                     /translation="MRPKILLTNDDGVNSGGLWAAYEALSAFADVTVVAPAVQQSAVG
                     RSISIFEPLRMNEVWIGDRSAYSVEGRPTDALLLGLYGLNLKPDLVVSGINLGENISY
                     ESITTSGTVGAAMEAANQGVPALAYSLQMSDQGNKFTDPRCHTQDFSQAKEVVAKLTR
                     IFLASGLPKNCHLVNINIPACRMEGYEVTTLGTRLFDTYVEKRIDPRGKPYYWINGDV
                     IYTPEPHSDVTALRNNRVSVTPLTLDNTAFAACGDLKKLLADQGL"
     gene            complement(2039..>2268)
                     /locus_tag="O0X12_02735"
     CDS             complement(2039..>2268)
                     /locus_tag="O0X12_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HJK53109.1"
                     /translation="AMVCGNVTDSRADGEVGSPGRALAEVMERNNLGSHVLMVSRIFG
                     GIKLGPAGVARAFREAGAGAVAEYQKTRKKL"
BASE COUNT          620 a          601 c          545 g          502 t
ORIGIN      
        1 acaatggaga cacaaaacat acatgacact tggcagcagc ggtgactccc cgaacaaaaa
       61 agaggaaaac cccacatcac cctctgaaac cagcagcaga ttcaaacaga tcatctgccc
      121 cggctgcttt gaagagatgg caaaagagga actcatcgga aacaaatgtc ccctctgcgg
      181 ataccggctc tcaccggagg agatggatga tgagatactt ggatcagggg atgaggaact
      241 cacatggatg ctcacacaga atctccagcg aacgcttctc tcctggctga tggatctggg
      301 agcagcacca cttgcagcgt atcggattgc atcaagggtc tgtgaacagg aaaatatccc
      361 ggtgaaaagc ggcaggacaa ccgcgttctc ctttactgca cggatgacac ccgaagaaaa
      421 agcagcggaa aaacagtgca aagtctgcgg cagaacattt accacaggag gcagaaaaat
      481 tgtctcgggt gatctctttg agccggaacc atccgttgag tacttctgtg atgactgcat
      541 cccgcaaaga taatattctc tttttccttt ttctcccaca gaggaattag acttgatccg
      601 tgttctacaa aactggttct gaacatcagg atcatacaga gataaaagta tatattcgca
      661 gatttcccaa gttgagagaa gatagtcatg ggcgattttc tgggtgattt aaagaagggc
      721 atcaacaaat ccgttgaaga cctgaacaaa tcggtgaaaa cactggagat tcagtcaaag
      781 atcagcagtc tcaaaaaaga ggcggatgcg gtatatgccg agataggaaa acgtgcagtt
      841 caggaaaatg gtctggaaaa atacggtgaa ctcggcgaca agctgcaggt actcttaact
      901 gacatcaaag cagaagaaga aaaactaccc gcagaggcct cagatgtaaa gaaaacaaaa
      961 atatgcccgg gatgcagcac agaaaacccg gttgatgcac ggttttgttc ctgctgcggt
     1021 gaaatgcttg aagatcagag tccctgatcc gcaagcagtt ttttgagatc cccacacgca
     1081 gcaaaggctg tgttgtccag tgtcagcggg gtcaccgaca cacgattgtt ccggagcgct
     1141 gttacatccg aatgcggctc tggcgtataa atcacatccc cgtttatcca gtagtacggc
     1201 ttaccgcggg gatcgattcg tttttccaca tacgtatcaa acagacgcgt tccaagtgtg
     1261 gtaacctcat agccctccat acggcaggca gggatattga tatttaccag atggcagttt
     1321 ttcggaagac cggatgcaag aaagatccgc gtcagctttg ctacaacctc tttggcctgg
     1381 gaaaagtcct gcgtgtggca gcgcggatcg gtaaacttgt ttccctgatc actcatctgc
     1441 agcgagtagg caagtgcagg aaccccctgg tttgcggctt ccatcgcagc gccgaccgtt
     1501 ccggaggtag taattgattc gtacgaaata ttttcaccga gattgatccc gctcaccacc
     1561 aaatcaggtt tgagattcag accgtaaagg ccaagcagca gcgcatcagt aggtctgcct
     1621 tccacactgt acgccgaccg gtcaccgatc catacctcat tcatcctgag cggttcaaag
     1681 atcgagattg atctgccgac cgcactctgc tgcacagcag gcgctaccac tgtcacatcc
     1741 gcaaacgctg acagtgcctc atacgctgcc cacagacctc cggagttcac accgtcatcg
     1801 ttagtgagaa gaatttttgg ccgcatgtat ttcctgctgc cttcaaggaa ctcagcagga
     1861 accgcatgat caaaggttgc acgaacatcc tgtgcccgca gataacggac catcacattc
     1921 actgcacagt ccttccactc atcgttccac cactggcagt tctccgcagt acaggtactg
     1981 ccgttcgcca tcgggcatcc gcgtaattga tccatgctac tatgttttct ttttggagct
     2041 ataacttctt ccgcgtcttc tggtactccg ccaccgcacc tgcaccagcc tcacggaatg
     2101 cacgggcaac acccgctggt ccgagtttga tgccgccgaa gatgcgggaa accataagaa
     2161 catgcgatcc gagattgttt ctctccatca cttcggcaag tgcgcggccg ggagaaccaa
     2221 cctctccgtc ggcacgactg tcggtaacat ttccacagac cattgcat
//
LOCUS       DALTZZ010000148         3974 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_21926,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000148 DALTZZ010000000
VERSION     DALTZZ010000148.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21926"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /locus_tag="O0X12_02740"
     CDS             <1..1133
                     /locus_tag="O0X12_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53110.1"
                     /translation="VGVIIVLAGLENVPRVKELDEIRAIYESEQSKEYEESDKTEKSP
                     KAQIANDELLFEDEMVMPIGVANRSAVPEVEDDIFELDDFTMQGTGDDVFEDEPRRVQ
                     YSADDADDVFEEVVSSPSQQRYMPPPQYTPQYVQQTQTGTIFDEPLSEDRPVIPKGKP
                     KAKEMPKITIAGPKKQPQMTGSTIMMPKRQKRVDNVLAGMADVGGKDKPKDSDNVLSG
                     KTRVGGNNRPKETITTNVLEGSVKVGNQRPKETDGGVRMSGGQRPKETMVPGVLEGSV
                     TVGTQRPKETDGHIRMAGSQRPKETDGHIRMASSQRPKETDGVVSGGSAQRPKEIGEY
                     PKVGDVQRPRELNKGIRVTSIPLPKNPSGKAVTKKSEKDKWL"
     gene            complement(1141..1572)
                     /gene="dcd"
                     /locus_tag="O0X12_02745"
     CDS             complement(1141..1572)
                     /gene="dcd"
                     /locus_tag="O0X12_02745"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HJK53111.1"
                     /translation="MLLSSSAIRARIGDGTLGITPFAEESLQPASYDLRAAEDTVMKT
                     GELSLIATMEFVSLPADLAATLRGRSSFGRKGVLLGAGYIDPGFRGNLTLCMVNMGKE
                     DIAVEKGTRIVQMLIHPVLGEVENVYNGNYQDSYGVVKSKI"
     gene            1678..1749
                     /locus_tag="O0X12_02750"
     tRNA            1678..1749
                     /locus_tag="O0X12_02750"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1711..1713,aa:Arg,seq:tcg)
     gene            complement(1777..2946)
                     /locus_tag="O0X12_02755"
     CDS             complement(1777..2946)
                     /locus_tag="O0X12_02755"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HJK53112.1"
                     /translation="MSIKHLVAVLLLASLLFCGIAAAAEATSLTINVYDNSAAHDPVK
                     DAKVVITKSGTSLSQTLYTTSDGIVEFPSVEYKSTYSVTVTKDGFDEQKFSIDVNTMG
                     KEYTVYLQKSNLVQVKVLNTDKSTPVAGAEISVDGLAMGTTNSAGVLHVSMEKGVYHN
                     IQVTADSYETYTSSQYIETDQTSLTITLSKSYFSPRILVYDADNDMKPVSLATVIIDG
                     KTVGTTDEYGRATLNDLTAGTYTLEVTKPNYNSYKNTVTFTEDSSDVIVELTYAVVPL
                     TVLVVDGNNPVAGATIYIDNLVTGLTDTTGKFTKDVEPGKTILITASKDGYATQSISW
                     SVTADQNNTVTVPITQNFPVALVGGIIAVIIIIGVVVFLVKAKGSSDNRGRKGSI"
     gene            complement(3091..>3974)
                     /locus_tag="O0X12_02760"
     CDS             complement(3091..>3974)
                     /locus_tag="O0X12_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HJK53113.1"
                     /translation="FTVQEIQQMTLKAGGVIVWGGATNIAPADDMIITYEYPLKIDAR
                     GQMLASIMAKKMAVGSDTCVIDIPVGPGTKVSDEAEGRVLAGELIELGRRLGIRVECA
                     ITYGGAPVGRNIGVNLEVAEALRILEGDSGPNSLMQKSAALAGMALEMTGKTAYGYGS
                     EAAIDLIRSGKALAKMRDIIEVQGGDPKIKSSDLLFGSNVFDVPAPNDGYVVSVKNRE
                     LINIARTAGSPADHGAGLHLHKKPGEAVKKGEPILTIYAERGWRLQRAIEEARVSMPV
                     VVEGMLLDRVPPIRRVA"
BASE COUNT         1021 a          957 c         1025 g          971 t
ORIGIN      
        1 atgtcggagt gatcatcgtt cttgccggtc tggagaatgt accgcgggtg aaggagttgg
       61 acgaaatacg agccatttac gaaagcgaac agagcaaaga gtacgaggaa agcgacaaaa
      121 ccgaaaagag tccaaaggct cagattgcga acgatgagct tctgttcgag gacgagatgg
      181 tgatgccaat cggtgttgcc aacagatctg ctgttcccga agtagaggat gatatctttg
      241 agctggatga cttcactatg cagggaactg gggatgacgt ctttgaagat gaaccgcggc
      301 gggtgcaata ctctgctgac gacgcagacg atgtctttga ggaggtggtc tcttctcctt
      361 cccagcagag atacatgcct cctccccagt atacgccaca gtatgttcag cagacacaga
      421 ccggaaccat ctttgatgaa cctctctctg aagatcgccc ggtgatccca aaagggaaac
      481 ctaaggcaaa agagatgccg aagatcacca tcgctggacc gaaaaaacaa ccgcagatga
      541 ccggcagtac aatcatgatg ccgaaacggc agaagagggt ggacaatgta cttgccggaa
      601 tggcagatgt tggcggcaaa gacaaaccaa aagactcgga caatgtcctc tccggtaaaa
      661 cccgtgtcgg cggtaacaac cggcctaaag agacaatcac gacaaacgta ctggaaggtt
      721 cggtgaaggt cggaaaccag aggccaaaag aaacggacgg cggtgtccgt atgtcagggg
      781 ggcagcgacc gaaagagaca atggttcccg gcgttcttga gggttcggtg actgttggta
      841 cacagcgtcc gaaggaaact gacggccata tcagaatggc aggaagccag agaccaaaag
      901 aaacggatgg tcacatcaga atggccagct cccagcgtcc aaaggaaaca gacggagtcg
      961 tgagcggggg ttctgcacag cgtcccaaag aaattggcga gtatcccaaa gtgggtgatg
     1021 tacaaagacc ccgcgaactg aacaaaggta tccgggtcac ctcgatacct ctgccaaaaa
     1081 atccctcagg aaaagcggtc acaaagaaaa gtgaaaagga caagtggctc taaaagccgt
     1141 ttatattttt gattttacca ctccgtaact gtcctgatag ttcccgttgt aaacattttc
     1201 cacttctccg aggacggggt gaatcagcat ctgtacaatg cgggtacctt tctcaaccgc
     1261 gatgtcctcc ttacccatgt taaccataca aagtgtcagg tttccccgaa agcccggatc
     1321 gatgtaacca gcaccaagca gcactccttt cctgccaaaa gaggaacggc cccggagtgt
     1381 tgcagcaaga tctgcgggaa gactgacaaa ctccattgtt gcaataaggg agagctcccc
     1441 ggttttcata accgtatcct cggctgcccg aagatcatag gatgctggct gaagtgactc
     1501 ttcagcaaag ggagtaatcc caagggtccc gtcgccgatg cgtgcacgga ttgcagagga
     1561 agaaagcaac atatcttttt tgttcgtttt cagtggtaat tgtattttgt gttgcgggga
     1621 tccggaaaaa tacgatagag taaaaaaggg ataaagatag atgaataata tcacattgga
     1681 tctgtagggt agtggatatc cttccgggct tcgaacccgg agatctgggt tcgatcccca
     1741 gcaggtccgt actgtgtctg ttttgaaaaa agttggttag atggatcctt ttctgccgcg
     1801 gttatctgac gatccttttg cttttacaag gaatacaaca accccgataa taatgatcac
     1861 cgcgatgatt ccgccgacaa gagcaactgg gaagttctgg gtaatcggca cggtcacagt
     1921 attgttttga tccgcagtta ccgaccagga gatactttgt gtggcataac catccttgga
     1981 tgcagtaatc agaatggttt ttcctggctc tacatcttta gtgaactttc ctgttgtgtc
     2041 ggtgagaccg gttacaaggt tgtcaatgta gatcgtagct ccggcgaccg gattgttacc
     2101 atcaacaacc agaacagtca gcggcacgac tgcataggta agttcaacga taacatcgga
     2161 ggagtcctcg gtgaaggtaa cggtgttttt gtaggagttg tagtttggtt tggtgacttc
     2221 cagtgtatat gttccggcag tgagatcatt gagggtggct cttccgtact catcggttgt
     2281 tccgacggtt ttaccatcaa taatgacggt tgcaagggag accggcttca tatcattgtc
     2341 agcatcatag acgaggatcc tcggggagaa gtacgacttg gaaagcgtga tcgtaagcga
     2401 ggtttgatct gtttcgatgt actgggagga ggtgtaggtc tcgtaagagt ccgcagttac
     2461 ctgaatgtta tgatacacgc ctttttccat agagacatga agtactcctg cgctgttggt
     2521 ggttcccatt gcaagtccgt caacggagat ctcagcaccg gcaaccggag ttgatttgtc
     2581 ggtgttgaga acctttacct ggacgagatt tgatttctgg agatagactg tgtattcttt
     2641 tcccatcgta ttgacgtcaa tggagaattt ttgctcatcg aatccgtctt tagtaacggt
     2701 tacactgtac gtggatttgt actcgacgga gggaaactct acaataccat cggaggtagt
     2761 atagagtgtc tggctcagag aggtcccgct tttggtgatt accacctttg cgtcttttac
     2821 aggatcgtgc gctgcactgt tatcatagac gttaatggta aggcttgtag cttctgcagc
     2881 tgcagctatt ccacagaata gtagggatgc cagcagaaga acagcaacaa ggtgtttgat
     2941 tgacatagat atattctgtt ttgggggtga tcgggtatta tttattcggt cggatggaat
     3001 gggctgaggt acgtgatttc gggccacaaa tctggtgggc agagattgtt tgtatgcttt
     3061 gaaaatagag ttgtgtggga aatacacata ttatgcaaca cgccggatcg gcggtacacg
     3121 atccagaagc atgccctcca ccacaaccgg catgctaact cttgcctcct caatcgcacg
     3181 ctgaagccgc catccccgtt ctgcatagat ggtcagaatc ggctctccct tctttaccgc
     3241 ctcccccggc tttttgtgca gatgcaggcc ggctccgtga tccgcaggcg aacctgcagt
     3301 cctggcgata tttatcagtt cacgattctt cacagagaca acatatccgt cgtttggtgc
     3361 cggaacatca aacacatttg aaccgaacag aagatccgag gacttgatct tgggatcgcc
     3421 tccctgtacc tcaatgatat cccgcatctt tgccagagct tttccgctcc ggattagatc
     3481 aatcgctgct tccgatccgt atccatatgc tgtctttccg gtcatctcaa gcgccatacc
     3541 cgcaagagcg gcactcttct gcatcagtga attgggtccg ctatcgcctt caagaattct
     3601 gagggcctcg gccacctcca ggttcacccc aatgtttctg cccaccggag caccaccgta
     3661 ggtaattgca cactcgacac gaatgccaag cctccgtcca agttcgatca actcacctgc
     3721 aagcactctt ccttcagcct catcggaaac ttttgttccc ggaccgaccg gaatatcgat
     3781 cacacaggtg tcagagccca ctgccatttt cttcgccata atgcttgcaa gcatctgtcc
     3841 gcgtgcatca atcttcagcg ggtactcata cgtgatgatc atatcatcgg cgggagcaat
     3901 gttggtagct cctccccaga cgatcacacc accggctttc agtgtcatct gctggatctc
     3961 ctgcaccgta aagg
//
LOCUS       DALTZZ010000149         3717 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_22101,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000149 DALTZZ010000000
VERSION     DALTZZ010000149.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22101"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            6..2213
                     /locus_tag="O0X12_02765"
     CDS             6..2213
                     /locus_tag="O0X12_02765"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HJK53114.1"
                     /translation="MADSVVDSILAARYYRVGEKSFEDVCRRVANALGETPEETKEYF
                     EAMMSLEFLPNSPTLMNAGTPLGQLSACFTLPVNDSLPEIFDAIRWGAIIHQSGGGTG
                     YNFSHLRPEGSPVRSTDGVASGPVSFMRVFNAATDVIKQGGRRRGANMGILNVWHKDI
                     MKFIKSKAKEGDFSNFNISVMVNDEFMKAVASGDLDREWLKTTDGETVTVREIWDGIV
                     EGVWKNGEPGILFYDTINRKNPTPQLGNIDTTNPCGEQPLLPFESCVLGSINLAKFIR
                     ADGVNYDALDKMTRMAVRFLDAVITKNVFPIEQIKEATNRTRKVGLGLMGVHDAMLML
                     RIPYDSEAGRNFCEEVMARINDVATEESERLGKEKGTFPAYKGSVWDKQGKIMRNAAL
                     TTIAPTGTISLLAGCSSGIEPVFSYAYTRRNTVGKTFIMVHPYFESELRRVIAGLGFT
                     GKEAEAKRDEVISHVHETGTVQDVLWLPDTFRAVFKTALDIQWKDHVLMQAVFQKHVH
                     ASISKTINMPFSATKEQVADAVLLAWKEGIKGMTLYRTGSREDVVLALERKEEKKEEP
                     AAAAEPKVVQQLPFSRPRELDGRTFLAQSGCCRLYITVNTMNGKPMEVFIRTVGAGCE
                     ASSNALGRSISTGLQNGVPYEKFVKQFAKVSCISALRNKNSEGMSCADVVGKCIELAA
                     TNQAITTLDNWTVQTVTPGAKKGNPCPECGEPLDFGEGCNMGICKHCGWSGCS"
     gene            complement(2302..3531)
                     /locus_tag="O0X12_02770"
     CDS             complement(2302..3531)
                     /locus_tag="O0X12_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53115.1"
                     /translation="MRPEVLYEYDLPLEVLDEIPADEEVLEVLYARSSENYHLPILIT
                     RLRIVCAYPETSVVYRVFQIDYVDLAEVHVKFTRGLSTVLTFTRLDGEMNVFDRVRNT
                     HEEIRAALLTLRDLMIEKVGGEWQFQHRQNLLTDEYAVKESDPVLSSVPTIPAEDLFE
                     DKEMPGLGCYEPTEKLFEHFPQDAEDGDVFEESAEILPETDAELKSVAMNSRIQRMIE
                     TAEIEAEHPEITDTGEVSVNDATVLESGPAVTDSQWANEDGGDAVILPDQHAGSRQSS
                     NGTRAAVPLVPADEDDSEVYVTPKPSPKKQKEEEAAAMIAEELDKLPVPEAKEPAKSP
                     LPALLPRAVPVQVPAVLPADKGVIPMSITLEPQDSFEEETVDACLESLKLLRDTGVIS
                     EEEYKDRCLRLFKTSSE"
BASE COUNT          941 a          966 c          988 g          822 t
ORIGIN      
        1 attttatggc ggactcagtt gtagacagca ttcttgccgc acgctattac cgcgtcggtg
       61 agaaatcatt cgaggatgtt tgcaggcgtg tggcaaatgc cctcggagaa actccggaag
      121 agacgaaaga atattttgag gcgatgatga gccttgaatt tctcccgaac tcaccaacac
      181 tgatgaatgc gggaaccccg ctcggacagc tgtccgcctg ttttacactc ccagtaaacg
      241 actctctgcc ggagattttt gatgcaattc gctggggtgc aatcatccat cagtcaggcg
      301 gcggtaccgg ctacaacttc tcccacctca gaccggaagg atcccctgtc cgctcaacag
      361 acggagttgc atccggccct gtgtcgttta tgcgtgtctt taacgcggca acagatgtta
      421 tcaaacaggg aggacgcagg cgcggcgcaa acatgggtat cctgaacgtc tggcacaagg
      481 atatcatgaa gttcatcaaa agcaaagcaa aggagggaga cttttcgaac tttaacatct
      541 ctgtgatggt gaacgatgag ttcatgaaag ccgttgcatc aggcgatctg gatcgcgagt
      601 ggctgaaaac aaccgacggt gagacggtga ctgttcgcga gatctgggac ggtattgtgg
      661 aaggtgtatg gaagaacggg gaaccgggaa ttcttttcta tgatacaata aaccggaaaa
      721 acccgacacc gcagctgggt aatatcgaca cgaccaaccc gtgcggagaa cagccgctgc
      781 tgccgttcga gtcctgtgtt ctgggaagta tcaacctcgc aaaattcatc cgtgccgatg
      841 gggtaaacta cgatgcactg gataagatga cgcggatggc ggtgcggttc ctggatgcgg
      901 tcatcacaaa gaacgtgttc ccgatcgagc agatcaagga ggcaacaaac cgtaccagaa
      961 aagtcggtct tggactgatg ggagtacacg atgccatgct gatgctccgc attccctacg
     1021 actcggaagc tgggcgcaac ttctgtgaag aggtcatggc acgtatcaac gatgtggcaa
     1081 cggaagagtc cgagcggctt ggaaaggaga aaggaacatt ccccgcatac aaaggatccg
     1141 tctgggacaa acagggaaag atcatgcgca acgcggcact tacgactatt gccccgaccg
     1201 gaacgatctc actgcttgca ggatgttcat caggaattga accggtgttt tcgtacgcct
     1261 acacccgccg gaacaccgtt ggaaaaacgt ttattatggt tcacccatac ttcgagtccg
     1321 agctgcggcg cgtaattgca ggccttggat ttaccgggaa agaagcagag gcaaagcggg
     1381 acgaagttat cagccatgtg catgagacag gaaccgttca ggatgtactg tggcttccgg
     1441 acacattccg tgctgtgttc aagactgcac ttgatatcca gtggaaggat catgtgctga
     1501 tgcaggctgt attccagaag cacgtgcatg catccatctc aaaaacgatc aacatgccgt
     1561 tctctgcaac aaaggaacag gtggccgatg cagttctgct cgcctggaag gaaggcatca
     1621 aaggcatgac gctgtaccgc accggctcac gggaggacgt ggtactggca cttgagagga
     1681 aggaagagaa aaaagaggag cccgcagcag cggcagaacc gaaggttgta cagcagctgc
     1741 cgttctcacg tccccgtgag ttggacggaa gaacattcct tgcccagtcg ggatgctgcc
     1801 gtctctacat tacggtaaac acgatgaacg gaaaaccgat ggaggtgttc atccggaccg
     1861 taggtgcggg atgcgaagca agcagcaatg cgcttggcag aagtatcagt actggcttgc
     1921 agaacggagt gccgtacgaa aagtttgtca agcagtttgc aaaggtgagc tgtatttctg
     1981 ccctgcgcaa taagaactcc gagggtatgt cctgtgcaga tgttgtcgga aagtgcattg
     2041 agcttgcggc aacaaatcag gcaatcacca cgctggacaa ctggacagta cagaccgtaa
     2101 caccaggagc aaagaaagga aatccctgcc cggagtgcgg tgaaccgctg gactttggcg
     2161 agggatgcaa catgggaatc tgcaaacact gcggatggag cggctgcagt taacccatcc
     2221 ctcctttccc atctttccag tagttgtcga accgatcaac tgcgaaaaac acttttttga
     2281 aatattgcaa ataaaaaaag attactcgct gcttgtcttg aacagccgga ggcagcgatc
     2341 tttgtactcc tcttctgaga ttacacccgt atcccgcaga agtttcagtg actcaagaca
     2401 ggcatcaacc gtttcctcct caaaggaatc ctgcggctca agagtgatcg acataggaat
     2461 cactcctttg tctgccggca ggacagcagg tacctgaaca ggcactgcac gcggcagcag
     2521 tgccggaaga ggagacttcg ccggttcttt tgcctccgga accgggagtt tgtcaagttc
     2581 ctcagcaatc attgctgctg cctcttcctc cttttgcttc ttcggcgaag gtttcggggt
     2641 aacgtacacc tcggaatcat cctcgtcagc cggtaccagc ggtacggcag cccgcgttcc
     2701 attactggat tggcgggaac cagcgtgctg atccggtaga atcaccgcat cgcctccatc
     2761 ctcgtttgcc cactgggaat cggtgacagc gggaccactc tcaagaaccg ttgcgtcgtt
     2821 gacggatacc tcccccgtat ctgtaatctc aggatgttct gcctcgatct ctgcagtttc
     2881 aatcatccgc tgaatccgcg agttcatagc aacggatttg agctcagcat cagtctctgg
     2941 aagaatctct gcggactcct caaacacatc tccgtcctca gcatcctgcg ggaaatgctc
     3001 gaacagtttc tccgttggtt cgtagcatcc aaggcccggc atctctttgt cctcgaagag
     3061 atcctctgca ggaattgttg ggaccgatga caaaacagga tcgctttcct ttactgcata
     3121 ctcatccgtc agcaggttct gcctgtgttg gaactgccat tcaccgccga ccttttcgat
     3181 catcagatcg cgcagagtca gaagggctgc ccggatctcc tcatgggtgt tgcgaacgcg
     3241 gtcgaagacg ttcatctcgc cgtcaagacg ggtgaaggtc agaaccgtgg aaagacctcg
     3301 ggtaaacttc acatgaacct ccgccagatc cacgtaatca atctggaaca cacggtacac
     3361 caccgatgtt tccggatatg cgcagacaat ccgcagacgt gtaattaaga taggcagatg
     3421 atagttttca ctactgcggg cgtagagaac ctccagcacc tcttcatcag ccggaatctc
     3481 atccagtact tcaagcggga gatcgtattc atagaggact tcaggtcgca cagttaccgt
     3541 ctcagatgtt gttatagttt gatgttattt cttagttaaa gttcgtaaac aggacgcaga
     3601 cagttactcc ttcttcctgc gaagtttccg gaaaaacgca gcccggccct ccgcattccc
     3661 agatatccac cacggcgtcc atgcaggatt ccagtgcaga acacaccagg tggcaac
//
LOCUS       DALTZZ010000150         4481 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_22530,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000150 DALTZZ010000000
VERSION     DALTZZ010000150.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22530"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1411)
                     /locus_tag="O0X12_02775"
     CDS             complement(<1..1411)
                     /locus_tag="O0X12_02775"
                     /inference="COORDINATES: protein motif:HMM:NF016219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HJK53116.1"
                     /translation="MSSLIFGIDIIRGSVRSGTIRPHYALVRVEDGEVISEEKNVTRF
                     RLMRYIRTELPEILAVDSIQEIAQGTADLYTFLAELPSGTKLVQVTGDGTKMETLPRV
                     AARYNLSFDKQNPMEEAKASALLASFGAGYEVLAYTGITDITVSRCRSPGRGGWSQNR
                     YVRKMHGAVRTHARDIEMKLISANLEYSMTVRRAFGGESRAVFTVYAPREQIPISPSK
                     GGDVQIRIAGRRRDKIAFQPLTKKPRYLIVGIDPGTTIGIAALDLDGTLIYLASTRLF
                     SAADLTREIATLGKPLIIASDKAEMPFGVEKIRRAFSAIAWNPKKDILIKEKYALAAG
                     YDFKNDHERDSLSAALYAYRTYKNKFESILKRAPAGVDTDELRAQIARGLSLEQALSQ
                     ISGTIPATTETQKETDTVTEIIFDERDERITRQEETIRRLRALVETLSRESTGKDKTI
                     SSLHRKLNAEREMQHTELLA"
     gene            complement(1408..2277)
                     /locus_tag="O0X12_02780"
     CDS             complement(1408..2277)
                     /locus_tag="O0X12_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HJK53117.1"
                     /translation="MPLSAEDIKNLHKYEVHILRALERMMHRYAWVPEDDLKAAVHLS
                     AAETKYRVGNLIHRDMVRSDAVPYKGYALVFKGYDALALSALAQKKTISALGTMIGVG
                     KESEIYEALGFGVVVLKLHKIGQRSFQTVRTNREYMPEKTHCPWIFASAKSAEREYEA
                     LKALNGKVNVPVPIDMNRHVIVMSFIPGVNLHRCRLEDPDKIWQEILSQVKSAYDAGF
                     IHGDLSEYNIMYDDTAVWIIDWPQWIPPDHVNADAVMRHDIETVAAFFAKKYQREYDI
                     DDAIRYVTGREVE"
     gene            2371..2964
                     /locus_tag="O0X12_02785"
     CDS             2371..2964
                     /locus_tag="O0X12_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53118.1"
                     /translation="MQCSRCGKEAVIVQPYSGVSLCAVHAARDLEAKAKRRIRKCGSI
                     SSGERLYVQESFGPESFALSVLLSRILAGRRDVSLVSDPSDATAVVSAETLDSSACCF
                     LQQIISGTTPEILSLPSRKVLDPLAVVPRVEVLQYARHHGWDGVDDRPRLSDPFTADI
                     CAFLEHFSVDHPSAAYALMQVRDALPQLYMERTNHAL"
     gene            2954..3694
                     /gene="nadE"
                     /locus_tag="O0X12_02790"
     CDS             2954..3694
                     /gene="nadE"
                     /locus_tag="O0X12_02790"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HJK53119.1"
                     /translation="MLCKDIGCAITRIKDLIRQTLWASGAKGVVVGISGGIDSAVAAA
                     IAVKALGPEHVLGVHMPSASSSPLDIADAKDFCDKFGIELLTVPLGDVVSAAFSDPYL
                     MDTPILRGNYTSRLRMATLYNIAAARGFLVCGTSNKTEYMIGYSTKWGDAAADIQPLL
                     HLWKKDVYTMADELDIPRSIIEKAPSAGFWEGQSDEDELGFSYQELDAALMSLEDHGF
                     VPQNALEEQVLALVTKSSHKRTSAVSLL"
     gene            complement(3896..>4481)
                     /locus_tag="O0X12_02795"
     CDS             complement(3896..>4481)
                     /locus_tag="O0X12_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HJK53120.1"
                     /translation="TKTVTFKVSVANNAEVNEYPVGLQIVYQDNYGVDQVSSPVIVGV
                     PVDGKVEFKVTNVESSLGPGDKGLIKVTYTNTGNIKVYNAEARLSAVDPFSSNDDSAY
                     LGDMEPGESVVGSYEVTVDSSATEKEYGLNTEIRYYDANDNSILSKSMKAVVDIKDQS
                     GPLAFFTSPIFIIIVVAIVLIGGYYVFMKRKGKV"
BASE COUNT          995 a         1088 c         1151 g         1247 t
ORIGIN      
        1 gtgcaagcag ttctgtgtgc tgcatctctc gttccgcgtt gagttttcta tgcagggacg
       61 agattgtctt gtcctttcct gtggattccc gtgaaagtgt ctccaccagt gctctcagcc
      121 tcctgatggt ctcttcctgt cgtgtaatcc gttcatcacg ctcgtcgaag ataatctcag
      181 taacagtgtc cgtttctttt tgtgtctctg tcgttgccgg tatagttccg ctgatctgag
      241 atagtgcctg ttcaagtgag agtccccgtg caatctgcgc ccgcagttca tcggtatcaa
      301 ctcctgcagg tgccctcttg aggatacttt caaacttgtt tttgtaagtc cggtacgcat
      361 acagtgctgc ggacagcgag tcacgttcat gatcgttttt gaagtcataa cccgctgcaa
      421 gggcatactt ttctttgatc aggatatctt ttttcgggtt ccatgcgatt gcggaaaatg
      481 cacggcggat cttctccacc ccaaacggca tctctgcttt gtcggatgcg atgatgagcg
      541 gctttccgag tgttgcaatc tcgcgggtca gatcagccgc ggagaataac cgcgtgcttg
      601 caaggtagat gagggttccg tcgagatcaa gggctgcaat gccgatggta gttccgggat
      661 cgattccgac gatcaggtac ctgggtttct ttgtcagcgg ttggaatgcg atcttgtcgc
      721 gtctgcgccc tgcgatgcgt atctgtacgt caccgccttt ggaaggagaa attggaattt
      781 gttcacgcgg tgcatagacc gtgaagactg cccggctttc tcccccgaaa gcacggcgga
      841 ccgtcatgga gtactcaaga tttgcagaga taagtttcat ctcaatgtca cgagcatgag
      901 tacggacggc accgtgcatc tttcggacgt atcggttctg actccatcct ccgcgccctg
      961 gtgaccggca gcgggagact gtgatgtcag ttataccggt gtatgcaagg acctcgtatc
     1021 ccgcgccgaa tgatgcgagg agtgctgaag cttttgcctc ttccattgga ttctgcttgt
     1081 caaatgagag gttgtaacgg gctgcaacac gcggcagtgt ttccattttt gttccgtccc
     1141 ccgtaacctg tacaagtttt gtaccagagg gcaattcggc aagaaatgta tacagatctg
     1201 cggttccctg tgctatctcc tgaatactat caactgcaag aatctccggc agttctgtac
     1261 ggatgtagcg catgagccgg aaccgtgtga cgtttttttc ttcagagatg acctctccgt
     1321 cctctacacg gacaagtgca tagtgcggcc ggatggttcc ggacctgacc gatcctcgaa
     1381 tgatatcaat tccgaatatg aggctgctca ttccacctcc ctgccggtaa cgtatcggat
     1441 tgcatcatca atgtcatact cccgctggta tttttttgcg aagaatgctg cgacggtttc
     1501 gatgtcatgg cgcataaccg catctgcatt cacatgatct ggcggaatcc actgcggcca
     1561 gtcaatgatc cataccgctg tgtcgtcata catgatgttg tactcggaga gatccccgtg
     1621 gatgaaccct gcatcatatg cggactttac ctgggagagg atctcctgcc agatcttatc
     1681 cggatcttcg agtctgcacc ggtgcagatt tactcccgga atgaaggaca tgacaattac
     1741 atgccggttc atatcgatgg ggaccgggac gtttactttc ccgttgagtg ctttaagtgc
     1801 ctcatactcc cgctcggcgg attttgcaga ggcaaatatc caggggcagt gggttttttc
     1861 cggcatgtac tcgcggttgg tacgaacggt ctgaaacgat ctctggccga tcttgtgaag
     1921 tttcagcaca accacaccaa atccaagagc ctcataaatt tcggactcct taccgacacc
     1981 gatcatggtg ccaagagcag atatggtttt tttctgtgct agggcagaga gtgcgagagc
     2041 atcgtatcct ttgaagacaa gtgcatatcc tttgtagggg actgcatcag atctgaccat
     2101 gtcccggtga atgaggttac caacacgata tttggtctct gctgccgaga ggtggacagc
     2161 ggccttaagg tcgtcttcag gaacccaggc ataacggtgc atcatcctct ccagtgcacg
     2221 aaggatgtgg acttcatatt tatggaggtt tttgatgtct tcggctgaca gaggcatgag
     2281 atattggttg actttgcagg gataaaagta tcatgtccga tgtgccgaag aacgaatcta
     2341 catatttgct gaacaccaac tgagtagtat atgcagtgtt cgcggtgcgg gaaagaggca
     2401 gtgattgtac agccctactc aggcgtatcc ctctgtgccg tacatgcagc ccgcgatctt
     2461 gaagcgaagg caaaacgaag gatccggaag tgcgggagta tctcgtctgg ggaacgactg
     2521 tatgttcagg agtcatttgg cccagaatca tttgcactct ccgttcttct ctcaaggatt
     2581 cttgcaggcc gccgtgatgt ttcgttagtg tcagatcctt ctgatgcaac tgcggtggtg
     2641 tcagcggaaa cactcgactc ctccgcctgc tgctttcttc aacagatcat ttcgggaacg
     2701 acgccggaga tactctccct cccttccagg aaagtacttg atcctctggc cgtagtccct
     2761 agggttgagg tgttgcagta tgcccgtcac catggatggg acggtgtgga tgatcgtcca
     2821 cgtttgtccg atccgtttac tgcggatatc tgtgcgtttc ttgaacactt ctcggtggat
     2881 catccttctg cggcctatgc actgatgcag gtccgggatg ccctgcccca actgtatatg
     2941 gagagaacca atcatgctct gtaaggatat cgggtgtgct atcacccgca taaaagatct
     3001 gatccgtcag accctctggg cttcgggtgc gaaaggtgtt gttgtcggaa tttccggggg
     3061 tattgactcg gcggtcgcag cggcgattgc agtaaaggcc cttgggccgg agcatgttct
     3121 tggtgtacat atgccgtctg cgtcaagcag tcccttggat attgcagatg ccaaggattt
     3181 ttgtgacaaa tttggtattg aactgctgac tgttcctctg ggagatgttg tttcagcggc
     3241 gttttctgat ccatacctga tggatactcc gatacttcgc gggaattaca cttcccgcct
     3301 gcgaatggca actctctata atattgcggc agcccggggc tttctggtct gtgggacctc
     3361 aaacaagacc gagtacatga tcggttacag tacgaaatgg ggtgacgcag cggcagatat
     3421 tcagccgctt ttgcaccttt ggaaaaaaga tgtatacacg atggccgatg agctggatat
     3481 tccacgttca attattgaga aggctccgag tgctgggttc tgggaaggcc aaagtgatga
     3541 agacgagctg ggtttttcct atcaggaact agatgcagca ctgatgagtc tggaggatca
     3601 tgggtttgta ccacagaatg ctcttgaaga acaggtcctt gcgctggtga ctaaaagttc
     3661 ccacaaacgg acttcggcag taagtctgct gtaagcactg ctttcgttca tttttttgtt
     3721 tctgttttgt caaggagcat tgttccttat ccctacggat agactctagg agataatgtg
     3781 gcatcgctcc ttagcgtaat cttatgcgaa cagatccgcc agtgatcttt ctgaaaaaca
     3841 gtgccaccac agcacttcaa aaattactgt tttaacaaaa aagattgggg gaggattata
     3901 ccttaccctt acgcttcatg aacacatagt agccgccgat taagacaatt gccacaacga
     3961 taatgatgaa gatcgggctg gtaaagaatg ccagcggtcc tgactgatct ttgatgtcaa
     4021 cgacagcttt catcgatttg gagagaatgc tgttgtcatt tgcatcatag tagcggattt
     4081 cggtgttgag accatactct ttttcggttg cagaactgtc aaccgttact tcatagcttc
     4141 ccactacaga ctctcccggt tccatgtcgc caagatacgc tgagtcatcg ttggaagaga
     4201 atggatccac tgcactcaga cgggcttctg cattgtatac tttgatattt cctgtattcg
     4261 tgtaggtgac tttgattaag cctttgtctc ccggtccaag cgatgactct acattagtta
     4321 ctttgaactc aacctttccg tcaaccggta ccccaacaat tactggggaa gagacctgat
     4381 caacaccata attgtcctgg taaacaatct gcaaaccaac cgggtattca ttcacctctg
     4441 cattgtttgc aacagatact ttgaaggtga ctgtcttggt a
//
LOCUS       DALTZZ010000151         1822 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_22889,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000151 DALTZZ010000000
VERSION     DALTZZ010000151.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22889"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="O0X12_02800"
     CDS             <1..882
                     /locus_tag="O0X12_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HJK53121.1"
                     /translation="ICSFTHSSTFCQGVEGLMGIEIPERANYLRTMWGEYSRIHSHLL
                     WLGLFADALGFESLFYNAWKIRESILNELEATTGGRVIQGVCKVGGVRRDVTNEFLSG
                     MDKRLESIEAEMQEMVNVFLNDYTMKKRLIGKGILKPEDAYNLGAVGPVARGSGLAQD
                     VRTSEYLAYQDIGFKPVTANGCDGYARCEVRCKELFQSAEIIHKVIAAMPDGEVAVPV
                     KGNPDGEYFSRSEQPRGEVIHYLRGNGTKYLTQFRVRTPTLTNVPSLVAMLAGAELAD
                     VPQIVLTIDPCIGCMER"
     gene            888..1268
                     /locus_tag="O0X12_02805"
     CDS             888..1268
                     /locus_tag="O0X12_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK53122.1"
                     /translation="MKMLKTILKQVTHKPVTTTFPAEPAKHFDATRGHIVFDPSKCRS
                     CTICMKRCPSQAIVVDRAKNTWTIDRFRCIICSQCVELCKFGGLSLATEYSACATPEE
                     RGTETFAITYVKPERPKKDAECGE"
     gene            1435..>1822
                     /locus_tag="O0X12_02810"
     CDS             1435..>1822
                     /locus_tag="O0X12_02810"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HJK53123.1"
                     /translation="MGERYWEVDAIRGIALIGMIVYHLLACMVIFHMIVEDEEFLTYY
                     GTINIASASFVLIAGVALILRHARKKGRTTQEYYRSIIIKALFLFAVAIGITIATWVG
                     AALVLHSDVYVKFGFLHMLSISILLSL"
BASE COUNT          498 a          411 c          477 g          436 t
ORIGIN      
        1 atctgcagct tcacccactc ctccacgttc tgtcagggtg tagagggact gatgggtatt
       61 gagattccag agcgtgccaa ctatctcagg acaatgtggg gggagtactc gcgtattcac
      121 agccacctgc tctggttagg cctcttcgca gatgcacttg gatttgagag cctcttctat
      181 aatgcatgga agattcgcga atcgatctta aacgagctgg aggcaaccac cggcgggcgg
      241 gtaattcagg gagtctgcaa agtgggaggt gtccgccgtg atgtgacaaa tgagttcctg
      301 tccggaatgg acaaacgact cgagagtatt gaggcggaga tgcaggaaat ggtaaatgta
      361 ttcctgaacg actacacaat gaagaaacgg ctgatcggga aaggcattct aaaaccagaa
      421 gacgcataca atcttggagc ggttggaccg gtcgcacggg gatcaggcct tgcacaggac
      481 gtccggacat ccgaatatct ggcgtatcag gacatcggat ttaagccggt tactgcaaac
      541 ggatgtgacg gatatgctcg ttgcgaagta aggtgcaaag aactgttcca gtccgcagag
      601 atcatccaca aagtgattgc cgcaatgccg gatggcgagg tcgcggtacc ggtgaaaggt
      661 aatccggatg gggagtactt cagtcgttca gaacagccac gcggcgaagt gatacattac
      721 ctccgtggaa acggtacaaa gtacctgaca cagttccgtg tgcggacacc gaccctgaca
      781 aacgttccgt cactggtcgc catgcttgcc ggggcagaac ttgcggatgt cccgcagatt
      841 gtactgacaa tcgatccgtg catcgggtgc atggagaggt gagactgatg aagatgctga
      901 aaaccatcct gaagcaggtt acacataaac cggtgacaac aacatttccg gcagagcctg
      961 caaaacattt tgatgcaacc cgtggccata ttgtattcga tccgtcaaag tgccggtcat
     1021 gtacaatctg tatgaaacgc tgcccgtcac aggcgattgt cgtggaccgt gcgaaaaaca
     1081 catggacgat tgataggttc cggtgcatca tctgtagcca gtgcgtggag ttgtgtaaat
     1141 ttggaggact gtcacttgca acagagtact cagcctgtgc aacaccagag gaacgcggga
     1201 ccgaaacgtt tgctatcaca tacgtaaaac cggaacggcc caaaaaagat gctgaatgtg
     1261 gagaataagt attcctgata catcaaaatg aactgatgtt actcagcatc attgtatatt
     1321 tttcccacat caaatttcaa aaatattttt tctgatctga catctaacaa tggatggaga
     1381 acttcgtcaa tatacaaaat atataatgat caaaaccaaa taggacggga gactatggga
     1441 gaaagatact gggaggtgga tgcaatccgg ggtattgccc tcatcgggat gattgtatac
     1501 catcttcttg cctgtatggt aatcttccat atgattgttg aggacgagga gtttctcacc
     1561 tattacggga ctatcaatat tgcatcagca tcattcgtcc tcatcgctgg tgtggcactg
     1621 atcctgcggc atgccagaaa aaaagggaga acaacccaag agtattaccg ttccattatc
     1681 atcaaagccc tgttcctgtt tgctgtcgca ataggaataa cgatagcaac ctgggttggc
     1741 gcagcccttg ttcttcacag cgatgtgtat gtgaagtttg ggttcctgca tatgctaagt
     1801 atctcaatcc ttttgtccct gc
//
LOCUS       DALTZZ010000152         1599 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_23105,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000152 DALTZZ010000000
VERSION     DALTZZ010000152.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23105"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="O0X12_02815"
     CDS             <1..206
                     /locus_tag="O0X12_02815"
                     /inference="COORDINATES: protein motif:HMM:NF017485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF815 domain-containing protein"
                     /protein_id="HJK53124.1"
                     /translation="IIITFQKPRADAYLEIVDSLAREYNLSVGKDQLHAGAEKYALRA
                     GGRTPRAAKQYIELLAANVLDTV"
     gene            407..>1599
                     /locus_tag="O0X12_02820"
     CDS             407..>1599
                     /locus_tag="O0X12_02820"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJK53125.1"
                     /translation="MTKERKRSLWNEKNMLLTGAEIIAEVLIEQGATTIFGYPGGAVL
                     NIYDALYEYRDKIRHIMTAHEEGASHAADGYARATGKTGVVLATSGPGATNLVTGIAT
                     AYMDSVPLVALTGNVGTSLIGRDSFQEVYIAGITMPITKHNFVVRDVSQLADILRDAF
                     RIARTGRCGPVLVDIPKDVTAAKTEFERAGPVEIPEPPMPVQEDILHAAEIINSAKRP
                     IIYFGGGVVSSGASEKLAALIHTANIPACHTIMGTGVLADDDPLNLGMIGMHGCFTTN
                     KAIDEADVVVAVGTRFSDRVALNTKKFAGRATIIHIDIDPSEISKNVPADCSLIGDVD
                     RILTELLPAISAAEHREWKDQIAEWQKADYVPEDSDTTLKPHQIIHTISRLAGEDAIF
                     VTDVG"
BASE COUNT          414 a          451 c          394 g          340 t
ORIGIN      
        1 gtattatcat caccttccaa aaacccagag ctgatgcgta tctggagatt gttgactctc
       61 ttgcccgtga atacaatctg tccgtgggaa aagatcagct tcacgcaggt gctgaaaagt
      121 acgcccttcg tgcgggcggc agaacaccgc gtgccgcaaa acagtacatt gaactccttg
      181 cagcaaatgt tttggatact gtataatccc catccctgtt tttcaaactt ctgtcagcgc
      241 gtccattgcg cgtctgtcca tctgtattcg accggacact ccgcgtaatc ggcggataga
      301 ttgttcagat gatgtggtgt gtcagtgtgt gccatgccaa aggtatacat acctcggacg
      361 aactacatat aagagcacgc gcaactcaaa aacagcgtga taggagatga ccaaagagag
      421 gaagagatct ctttggaatg aaaaaaacat gttactaact ggtgcagaga tcatcgcaga
      481 agttctcatt gaacagggag ccaccaccat cttcgggtat cctgggggtg cggttctcaa
      541 catctacgat gctctctatg aataccgtga caaaatccgt cacatcatga cagcccacga
      601 ggaaggagcc tctcacgcgg ctgacggata cgcacgcgca accggcaaaa ccggcgtcgt
      661 acttgcaaca tccgggcccg gagcaaccaa cctcgttaca ggaattgcta ccgcatacat
      721 ggacagcgtc cccctcgttg cactaaccgg caatgtcgga accagtttga tcgggcgtga
      781 cagcttccag gaggtgtaca tcgccggcat caccatgccc atcaccaaac acaactttgt
      841 agtgcgtgat gtatcccagc ttgcagacat cctccgggac gctttccgga tcgcccgtac
      901 cggccgctgc ggtccggtac tcgttgacat tcccaaggat gtcaccgccg caaaaaccga
      961 gtttgaacgg gccggaccgg tcgagattcc ggaacctcca atgcccgtac aagaggacat
     1021 cctgcatgcg gcagagatca tcaacagtgc caaacgtccg atcatctact ttggaggcgg
     1081 cgttgtgagc agcggagcat ccgaaaagct tgcagccctc attcataccg caaacattcc
     1141 ggcatgccac actattatgg gaaccggtgt tcttgcagac gatgatccgc tcaaccttgg
     1201 tatgatcggc atgcacggct gctttaccac caacaaagcc atagacgagg cagatgttgt
     1261 cgttgctgtt ggaacacgct tcagcgatcg ggtcgcactc aacaccaaaa agttcgccgg
     1321 acgtgcgacc atcattcaca ttgacatcga cccaagtgaa atcagcaaaa acgttcctgc
     1381 ggactgcagc ctcatcggtg acgtggacag aatcctcaca gaacttctgc ccgcgatctc
     1441 agctgccgaa caccgcgaat ggaaagatca gattgccgag tggcaaaagg ccgattatgt
     1501 acccgaggac agtgacacta ccctcaaacc gcatcagatt atccatacga tctcgcggct
     1561 tgcaggtgag gatgccatct tcgttaccga tgtgggtca
//
LOCUS       DALTZZ010000153         4258 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_23193,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000153 DALTZZ010000000
VERSION     DALTZZ010000153.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23193"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="O0X12_02825"
     CDS             complement(<1..1335)
                     /locus_tag="O0X12_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HJK53126.1"
                     /translation="MEIKYVTTTCPYCGAGCTFNLVVKDGKISGVQPCQRGPVNEGKL
                     CPKGIFGWEFIVSEDRLKTPLIKDKATGEFKPATWDEALTVIAENFKKYSPEEIAVIS
                     SARCCNEDNYAMQKFARIVLKTPNVDHCARLCHAPTVAGLNMVFGSGASTNSFDDLAI
                     ADFVFIIGSNNFEAHPLAGRRIMQAKEKGAHILVCDPRVTPTAKQADLHIQHYPGTDI
                     QLLNCLMKQIIERGWVDEEFVKNRTNGYEELKACVTQDKYSIENTSVVCGVPAEKIEQ
                     ALEWLHECQHKTAFVHCLGITQHTVGVDNVRSIAFVQTLLGNIGKPGCGVNALRGQNN
                     VQGSCDMGALPNVYAGYLKVTDPEAAKKVAAYWGVDEIPNGKLGLHIPEMLETFEEHP
                     DKLKCLYLMGENPVMSDPNSKGVEQAMKNCEFLVVQDIFETETTKYADVVLPG"
     gene            complement(1616..2974)
                     /gene="cfbB"
                     /locus_tag="O0X12_02830"
     CDS             complement(1616..2974)
                     /gene="cfbB"
                     /locus_tag="O0X12_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide synthase"
                     /protein_id="HJK53127.1"
                     /translation="MRSLLISGDRSGAGKTSITLAIAGLLAQESPVQTYKVAMDYIDT
                     SYLAGVTGRPSYNLDTFVQTPEETSGLFSYGSVGAEYGIVEGVRGLYEGVDALTDVGS
                     TASVAKMFDLPVILVINARSITRSAAALVKGFAAFDPDIRIEGVILNNVMGERHIGKA
                     TKAVEHYCGIPVLGAVPRRPEMELAMRHLGLVPYREGSDNAEFHDRISFITNVIGEYV
                     DIDRIRETAQDRTPRPNAVCEMLTAPSIPTMRIAVAYDEAFNFYYGELTAVLRSLGAE
                     VVHFSPIHDRLPDADGYIFGGGYPELFLEELEHNDVMREAVRETAENNVPIYAECGGL
                     MYLTRSLTLETGWQGRDDRISCEMCGVFAGDTCMPVRKALGYVIGTASLPSGDFAFRG
                     HEFHYSGISMDAGVHYAYRLSRGTGIQNGCDGALVHRTLGAYTHLMPVSSRSMLKSFF
                     GL"
     gene            complement(2971..4035)
                     /gene="cfbD"
                     /locus_tag="O0X12_02835"
     CDS             complement(2971..4035)
                     /gene="cfbD"
                     /locus_tag="O0X12_02835"
                     /EC_number="6.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HJK53128.1"
                     /translation="MRYVQPRPSSIVASLYTLRDLDVDLAILHGPSGCSFKHARLLEE
                     DGIRVLTTSLGDEEFIFGGQRILEDVLRYAEEEFAPKKIAVVGTCVSMIIGEDMEAAI
                     EASGISTPAIAVNIHAGFRENIDGVIAALEPAAAAGWISPEELERQRRVLAAANATER
                     ARGAACKTYIPPSRGDLKHVAATELLALARSGKRGLAIMNAKKETAYMFADELLALHD
                     AVPDANITYVANLEERGLPKVRGDAAAIFAEMRGRGVDPVLCGALDEYGENGSAIAEI
                     VAEDHPDFVLLVGVPHAVVPEALQGITVFSVTNGPRQVSPLKEQGHAYVMVEIDLHPK
                     TLGVHEIVESEFGAVLRSVA"
     gene            complement(4043..>4258)
                     /locus_tag="O0X12_02840"
     CDS             complement(4043..>4258)
                     /locus_tag="O0X12_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53129.1"
                     /translation="VCEGFPVNEIRRAVSLVSPAHLVIITGSDEYADQFPDADTICTT
                     LSAATDSALDLAKTSGGSVLLAAKTWR"
BASE COUNT         1080 a         1158 c         1005 g         1015 t
ORIGIN      
        1 gccggggaga acaacatcag cgtacttcgt ggtttcagtc tcaaagatat cctggacaac
       61 aaggaactca cagttcttca ttgcctgttc gacacccttg gagttcgggt cggacataac
      121 cgggttttct cccatgagat agagacactt cagcttgtca gggtgctcct caaaggtctc
      181 aagcatttct gggatgtgta atccaagttt gccgttcggg atctcgtcaa cgccccagta
      241 ggctgcaacc ttcttagctg cttcggggtc ggtgacttta aggtaaccag cgtaaacgtt
      301 cgggagtgca cccatatcgc aggaaccctg cacgttgttc tgaccgcgga gtgcgttgac
      361 accacagcct ggcttgccga tgttaccgag aagggtctgg acaaaggcaa tggaacggac
      421 gttgtcgaca ccaacagtgt gctgcgtgat accaagacag tggacaaagg cagtcttgtg
      481 ctggcactcg tggagccact caagggcctg ctcgatcttt tctgccggta caccacatac
      541 gacagaggtg ttttcgatgc tgtatttgtc ctgagtaacg caggctttga gctcctcgta
      601 accgttggtg cggttcttca cgaactcttc gtcaacccag ccgcgctcaa tgatctgctt
      661 catcaggcag ttaaggagct ggatatcggt tcctgggtag tgctgaatgt gcaggtcagc
      721 ctgtttggcg gtcggagtga cacgcgggtc acagaccagg atgtgggcac ccttttcctt
      781 agcctgcatg attctgcggc ctgcaagcgg gtgggcttcg aagttgttcg aaccaatgat
      841 aaacacgaag tctgcgattg caaggtcgtc aaaagagttg gtcgatgcac cggatccaaa
      901 gaccatgttc agaccggcga cggtcggtgc gtggcacaga cgggcacagt ggtcgacgtt
      961 cggggtcttt aagacaatac gggcgaactt ctgcattgcg tagttgtctt cgttacaaca
     1021 gcgggcagag gagataactg cgatctcttc ggggctgtat tttttaaagt tctcagcaat
     1081 gacagtgaga gcttcgtccc aggttgcggg cttgaattca ccggttgctt tgtctttaat
     1141 cagaggggtc ttaagacggt cttcggagac gatgaactcc cagccgaaga tacctttcgg
     1201 gcagagttta ccttcgttga ccggaccgcg ctggcacggc tggacaccgg agatcttacc
     1261 gtcctttacc accaggttaa aggtacagcc tgcaccacag taggggcagg tcgtggttac
     1321 atacttgatt tccatggata aacctcggat actataacct ctgtaaagag gacataaata
     1381 cctatccaga tttttctgat ttttgtgtat tattggtggg ttgaatgttt ttcatcagaa
     1441 ctgagaattc aatatgttgt gttaataaga ctgtgagatt aaacagatgt gaacaataag
     1501 cgcgcatttg cggcagatga gcgcagattt atccaaaagc aggagtgtct ataaacgaat
     1561 aggaacctga tctgttctaa aaaccggagg agaaagtggg cactccgaac agatctcaca
     1621 gtccaaagaa actcttcagc attgaccggg acgataccgg catcaaatgt gtatatgcac
     1681 ctaatgtccg atgtaccaat gcaccatcac atccgttttg aatacccgtt ccgcgggaaa
     1741 gtcggtacgc atagtgtacc cctgcgtcca ttgaaatacc actgtaatgg aactcatgcc
     1801 cacggaatgc aaaatcacca ctcggaagtg atgcagtccc tataacatag cctaaagcct
     1861 tacgaacggg catgcatgta tctcccgcaa atactccgca catttcgcac gaaatccgat
     1921 cgtcgcgacc ctgccagcct gtctcaagcg taagcgaccg cgtcaagtac atcagacctc
     1981 cgcactccgc gtagatgggt acattgttct ccgcagtctc gcgtaccgcc tcacgcataa
     2041 catcattgtg ttcaagctcc tccaaaaaca actcgggata cccacctcca aaaatataac
     2101 cgtccgcatc aggtaagcga tcatgaatgg gactgaaatg cacgacctct gctcccagac
     2161 ttcgcaagac cgcagtcagt tctccataat agaaattaaa cgcctcatca tatgcaactg
     2221 caatgcgcat tgtgggaatt gatggggcag tgagcatttc gcacaccgca ttcggacgcg
     2281 gcgtcctgtc ctgtgcagtt tcgcgaatcc tgtcaatatc cacatactcc ccaatcacat
     2341 ttgtaataaa ggaaatccta tcatggaact ccgcattgtc cgaaccctca cggtagggaa
     2401 caagacccag atgccgcatt gcaagctcca tctccggcct tcgcggaaca gcccccagga
     2461 ccggaatacc acagtaatgc tcaaccgcct tcgttgcttt tccgatgtgg cgttcaccca
     2521 ttacattatt caggataact ccctcgatac gaatgtccgg atcaaatgcg gcaaaccctt
     2581 tcaccagtgc agcagcactg cgtgtaatac tcctggcatt gatcacaaga atcaccggca
     2641 gatcaaacat ttttgcaacc gatgccgtac tgccgacatc agtcagtgca tccacccctt
     2701 catacagacc gcgtactcct tctacgattc cgtactccgc tcccaccgaa ccataactga
     2761 acagaccaga tgtctcttct ggagtttgca caaatgtatc cagattatag gaaggacgac
     2821 cggttacccc tgcaagatac gatgtatcga tataatccat tgcgactttg tacgtctgta
     2881 ctggagattc ctgagcaagc agacctgcaa ttgcaagtgt tatacttgtc ttccccgcac
     2941 cggaacgatc acctgaaatc agcagagacc tcatgcaaca ctccggagca cagccccgaa
     3001 ctcactttcc acaatctcat ggacaccaag cgtttttgga tgcagatcga tctcaaccat
     3061 cacatacgca tgtccctgct ctttcagggg ggaaacctgc cgcggtccat ttgtaacaga
     3121 aaacactgtt attccctgaa gtgcctcagg aacgacggca tgtgggacac ccaccagcag
     3181 aacaaaatca gggtgatcct ctgcaacaat ttctgcaatc gcagagccgt tttctccgta
     3241 ctcatccaga gctccgcaga gaacaggatc cacaccacgc ccacgcatct ctgcaaaaat
     3301 tgctgcagca tccccccgga cttttggaag tccccgttcc tcaaggtttg ccacatatgt
     3361 gatgtttgcg tcaggaactg catcatgcag cgcgaggagt tcatccgcaa acatgtaggc
     3421 ggtctccttt tttgcattca taatagcgag accccgtttg ccggatctgg caagtgcaag
     3481 aagttccgtt gctgccacgt gtttcagatc gccgcgtgac ggtggaatgt acgtcttaca
     3541 ggcagccccc cgtgcccgtt ccgttgcatt tgctgccgca agtacacgcc gctgccgctc
     3601 aagttcttcg ggactgatcc agccagccgc ggccgcaggt tcaagagcgg caattactcc
     3661 atcgatgttc tcacgaaatc ccgcatgaat gtttaccgca attgcaggag tagagatccc
     3721 ggatgcttcg atggctgcct ccatatcctc tccgatgatc attgagacac aggtacccac
     3781 aacagcaatc ttctttggag caaactcctc ttctgcatac ctgagtacat cctcaagaat
     3841 ccgctgaccg ccaaagatga actcttcatc accaagtgac gtggtcagaa cacgaatacc
     3901 gtcctcttcc agaagtctgg catgtttaaa tgaacaccct gacgggccgt gtaaaattgc
     3961 aagatccaca tccaagtccc gcagcgtata caatgatgca acgatcgaac tcggacgggg
     4021 ttgaacatat ctcattgtca tattatctcc atgtttttgc tgcgaggaga acagaacctc
     4081 cggatgtttt ggcaagatcc agtgcagaat ccgttgcagc agaaagggtt gtacatattg
     4141 tatccgcatc ggggaattga tcagcgtact catcagaacc ggtaatgatc accagatgtg
     4201 cgggactgac caaagaaacc gcccgacgaa tctcatttac aggaaaacct tcacagac
//
LOCUS       DALTZZ010000154         3520 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_23271,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000154 DALTZZ010000000
VERSION     DALTZZ010000154.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23271"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /gene="frhG"
                     /locus_tag="O0X12_02845"
     CDS             <1..610
                     /gene="frhG"
                     /locus_tag="O0X12_02845"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit gamma"
                     /protein_id="HJK53130.1"
                     /translation="EMDVALVEGSVCLNDECSMEEILETREKAKIVVALGGCACYGNT
                     TRFCRGGQQNQPQHEAFLPIGDLIKVDVYIPACAPTPQQIRNVCLMAYLLLKGNDEQK
                     ALATAYLTPLMNLAAAGTEACGCDLMTEVINQGLCCGCGSCAAACTVRAITMQYGRPQ
                     IERDLCIKCGACYAQCPRSFFSFDVCGQYEAITNLIGEVMKP"
     gene            631..1503
                     /gene="frhB"
                     /locus_tag="O0X12_02850"
     CDS             631..1503
                     /gene="frhB"
                     /locus_tag="O0X12_02850"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit beta"
                     /protein_id="HJK53131.1"
                     /translation="MEYLGQYKAVYKAKAGCEDILSKAQDGGIVTAMFAYALETGIID
                     GAIVAGPGEEAYKPEPMIATTVDELLAARGTKYSISPNMSLIKEATRSYGLDKIGIVG
                     TPCQIQAVRKAQLYPVGLRDVPDKIALALGIFCMENFPYQGLYQMVEDHCATKIDNVT
                     KMDIGKGKFSVYTERGVNAQIPLKVTHKYEQPGCHVCLDYVANLADISTGSVGTPDGW
                     STVFVRSAAGEAAFNKAVEAGWIIAESMDGVKPGLELVTKLATEKITKNQKYIEHRAH
                     ADHPALKPLRNPYI"
     gene            1625..2512
                     /locus_tag="O0X12_02855"
     CDS             1625..2512
                     /locus_tag="O0X12_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53132.1"
                     /translation="MTEETDRAADITPEKTNPPKRRRVTKKKTETETVQAPTETPEKQ
                     TENKIELAAIEATTETEEKATEEAERPHKAVPEQFLNDLAQFADILEKTPALLDPIRE
                     VFEYHLVDPIDRKTFPRMVNAMSMLLGPSATMVVMTACHVNSAAFFEDLLYAIKDEKS
                     RGAVWIIQHLTALYGNRVQQAYTLSSGTMDEDWHTVDVNTYKREGENAYWIVDVKMML
                     YSGKESQIKMTPDSIFQLVEILAAELGRNIPIENVDPGLVKKCEENFTIFYEKFYGAK
                     KCEETEKTDGEEEHPAGYA"
     gene            complement(2565..>3520)
                     /locus_tag="O0X12_02860"
     CDS             complement(2565..>3520)
                     /locus_tag="O0X12_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HJK53133.1"
                     /translation="GLVSCVSVAEYKDDTIKKHELTRYDKEEDRTRHIDAVSAHTGQV
                     FLLYKDPGNIHAYISGLADAMQPFGEYRSEGGNLHQIYRVTDHADIAKLETMFAEVPN
                     LYIADGHHRTKSSVNVYDRRKAEGRCTPDAERFMSVLFAHDKVRIYGYHRLVRDLAGM
                     NADQFLSDLALRFTVTPIKKVDITKNTVPPLADTDGSTHIIHLYLESQWYELTRPVDP
                     KADAIARLDVSVLQESVLDDMLGIIDPRGDPRISFVGGIVPLSDVTREVDAGEFSAAF
                     IMQPVTAQGVIDVADNAMIMPPKSTWFEPKLLSGMVIHEIH"
BASE COUNT          970 a          888 c          910 g          752 t
ORIGIN      
        1 agagatggat gtcgcacttg tcgagggttc cgtctgttta aacgacgagt gctcaatgga
       61 agagattctg gaaacccgtg agaaagcaaa gattgttgtt gctctcggag gctgtgcatg
      121 ttacggcaac accacccgct tctgccgtgg tggccagcag aaccagcccc agcacgaggc
      181 attcctgccg atcggtgacc tgatcaaggt tgatgtatac atcccagcat gtgcaccaac
      241 cccgcagcag atccgcaatg tgtgcctgat ggcatacctg ctgctgaaag gcaacgatga
      301 gcagaaagca ctcgcaactg catacctgac cccgctgatg aaccttgcag cagcaggaac
      361 tgaggcatgc ggatgcgacc tgatgaccga agtcatcaac cagggcctct gctgcggatg
      421 cggcagctgt gctgcagcct gtactgttcg tgcaatcaca atgcagtacg gcagaccgca
      481 gatcgagcgc gatctgtgta tcaagtgcgg cgcctgctat gcacagtgcc cgagaagctt
      541 cttcagcttc gacgtgtgcg gacagtatga agcgattacg aacctcattg gagaggtcat
      601 gaaaccctga gggtcccgga ggagaaaaaa atggaatatc ttggacaata caaggcagtc
      661 tacaaagcca aagccggctg tgaagacatt ctctcaaagg cccaggacgg aggaatcgtc
      721 actgcaatgt ttgcctacgc acttgagaca ggcattattg acggagcaat cgtcgcagga
      781 ccaggagaag aggcatacaa gcccgagccg atgatcgcaa ccaccgttga cgagcttctc
      841 gcagcacgtg gaacgaaata ctcgatctct ccgaacatgt ccctgatcaa ggaggcaacc
      901 cgcagctacg gtcttgacaa gatcggtatc gtcggtaccc cctgtcagat tcaggcagta
      961 agaaaagcac agctctaccc ggtcggtctc cgtgacgttc cggacaagat tgctcttgct
     1021 cttggtatct tctgtatgga gaacttccca taccagggtc tctaccagat ggttgaggac
     1081 cactgtgcaa ccaagatcga caatgtcacc aagatggaca ttggaaaggg caagttctct
     1141 gtgtacaccg agcgcggtgt gaacgcacag attccgctga aggttaccca caagtatgag
     1201 cagccaggat gccacgtatg tcttgactac gtcgcaaacc ttgcagatat ctcaaccggt
     1261 tccgtaggaa ctccggatgg atggtccact gtcttcgtcc gctccgcagc aggagaggca
     1321 gccttcaaca aggcagtcga agcaggctgg atcattgctg agtcgatgga cggtgtcaaa
     1381 ccaggtctgg aactcgtgac caaactcgca accgagaaga tcaccaagaa ccagaagtac
     1441 atcgagcacc gtgcccacgc ggaccacccg gcactgaaac cactgcgcaa cccatacatc
     1501 taaccgtgtc ctaagggttg tacatgtacc cacccctctc gggtacaacc tttttttgaa
     1561 aatctgatga ctgaagttct gcagaccgat gaggtttttg tatatcggat ccaaatgagt
     1621 tagtatgact gaggagacgg accgagcagc agatattacc ccggaaaaaa caaacccacc
     1681 aaaacgacga agggttacaa agaaaaaaac tgaaacggaa acggttcagg ctcccacaga
     1741 aactccagaa aaacagactg aaaacaaaat tgagcttgcc gcaattgagg ccacgactga
     1801 aacagaggaa aaagcgacag aagaggcgga gagaccgcac aaagcagttc ctgaacagtt
     1861 cctgaacgat cttgcacagt ttgccgatat tctggaaaag acacctgcac tgctggatcc
     1921 aatccgcgag gtgtttgagt atcatttggt ggatccgatc gatcgcaaaa cattcccccg
     1981 catggtaaat gcaatgtcaa tgctgcttgg tccgtcagca acaatggttg tgatgacagc
     2041 ctgccatgta aactcagcag cattctttga agatctgctg tatgcaatca aggatgagaa
     2101 aagccgcggt gctgtgtgga tcatccagca cctgactgca ttatatggaa accgtgtaca
     2161 gcaggcatac accctgtcat caggaacaat ggatgaggac tggcatacgg tggatgtcaa
     2221 cacctacaag cgcgaaggag agaacgcgta ctggattgtt gatgtaaaaa tgatgctcta
     2281 ctcagggaaa gagagccaga tcaaaatgac gccggactca atcttccagc tcgtggaaat
     2341 tcttgcagca gagcttggaa gaaacattcc aatcgaaaat gtggatccgg gtcttgtgaa
     2401 aaaatgcgag gagaacttta caatctttta tgaaaagttc tacggcgcaa aaaaatgtga
     2461 agagacggaa aaaactgacg gggaagagga acatcccgca ggatatgcat aatttttttc
     2521 aaacgagacg ctaatcagcg ggaattctat cccattaaaa aatgttagtg tatttcatgg
     2581 atgaccatgc cggagagaag ttttggttca aaccacgtgg acttcggcgg cataatcatc
     2641 gcattgtctg caacatcgat aactccctga gcggtcaccg gctgcataat gaatgcagct
     2701 gagaactcac cggcatcaac ctccctggta acgtccgaca ggggaacaat accgccgaca
     2761 aaggagatgc gggggtcccc gcggggatca atgatgccga gcatatcatc gagtactgat
     2821 tcctgcaaca ctgaaacatc tagccgcgca atcgcatccg cttttggatc aaccggacgt
     2881 gtcagttcat accactggct ctccagatag agatgaatga tatgtgttga tccgtcagta
     2941 tctgcaaggg gtggaactgt atttttcgtg atgtcaacct tcttaatcgg cgttactgta
     3001 aaccgcaggg caagatcgct gaggaactga tcggcattca tgcctgcaag atcacgaacc
     3061 agccggtggt acccgtagat ccgcactttg tcatgagcaa acaaaacgct catgaaccgt
     3121 tcggcatccg gcgtgcatct tccctcagcc ttcctgcgat cgtacacatt gacagatgac
     3181 ttggtacggt gatggccgtc tgcgatgtag aggttgggta cctcggcaaa cattgtctca
     3241 agttttgcga tatcagcgtg atctgttacg cggtagatct ggtgcaggtt cccgccctca
     3301 ctccggtact caccaaacgg ctgcatggca tccgcaagac ccgagatgta ggcatgaata
     3361 tttcccggat ctttgtagag caggaacacc tgaccggtgt gtgctgagac ggcatcgatg
     3421 tgacgggtac gatcctcttc tttgtcgtag cgggtcagtt catgcttttt gatcgtatca
     3481 tccttgtact cggcaacact cacacaggag acaagaccgg
//
LOCUS       DALTZZ010000155         5008 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_23387,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000155 DALTZZ010000000
VERSION     DALTZZ010000155.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23387"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            178..861
                     /locus_tag="O0X12_02865"
     CDS             178..861
                     /locus_tag="O0X12_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HJK53134.1"
                     /translation="MMDQDAIAHLLDILGNRNRRRILDLLRQKPCFVTEISERLVINP
                     KAVIEHLAIMQKEDVISFYQDEKRRKYYYLVQDFELSVQMTKPEQPPQSIAAASDQTG
                     SDASAPPLTDKILMIRRLLDSREKLIENLEAVEKDIDEMMNDVIVSGRSVFQNSIESE
                     ILLALIYSPLTPIELSDTIGHPIPEVTGSLRTLASKGYVEAEGGRYRIKGTQKAITLA
                     AGPALPMKI"
     gene            905..975
                     /locus_tag="O0X12_02870"
     tRNA            905..975
                     /locus_tag="O0X12_02870"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:937..939,aa:Gly,seq:ccc)
     gene            985..1659
                     /locus_tag="O0X12_02875"
     CDS             985..1659
                     /locus_tag="O0X12_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5612 domain-containing protein"
                     /protein_id="HJK53135.1"
                     /translation="MDESHNYAISLITENQVGILRDIGSVCAEHNANIVLTQQETVSR
                     GPDSGYAWVDLEITACGRIQDLVEALQQIPGVRDVKLQDTFRTIFGKRVIIMGGGAQV
                     AQVAMGAVNEADRHNIRGERISVDTIPLVGEENLASAVSAVARLPRASILVLAGSIMG
                     GEITEAIHAVQAEGIPVICLKMVGSASAAADLIVTDPIQAGVMAVMHISSIGVFDLNR
                     VRGREF"
     gene            1676..2332
                     /locus_tag="O0X12_02880"
     CDS             1676..2332
                     /locus_tag="O0X12_02880"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HJK53136.1"
                     /translation="MTKPTESSNPCCSSPYSYERAEVTPALEAVIEKAVRVLARDGLV
                     VYPTETIYGLGADALSEVAVYKVYEAKQRPMGKPISVAVSDIEMIYGVAYVDEFAERF
                     IQKFLPGPVTVVLPVKSCLPPDLSGGTGTIGIRYPDHAVAQAIIAGLDSPITSTSANI
                     SGEVSPVTSDQINVPHDYLIDGGKLPGTPSTVINLATKKIERPGAMLEEIASFLKEEL
                     "
     gene            2387..2881
                     /locus_tag="O0X12_02885"
     CDS             2387..2881
                     /locus_tag="O0X12_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HJK53137.1"
                     /translation="MTCTEYRKPSLPILIGSVVLCFIAAALGSLVTITGTDSWFMTEL
                     IKPEWQPPSYLFGPVWTVLYLLMGIALALILAQGAERREVRVAAGVFGVQLVLNVLWS
                     YLFFGWQMIGAAAVEIILLWIVIGVTMYLFYRIRPAAAYLLIPYIAWVTFAAVLTATI
                     WTLN"
     gene            complement(2891..3358)
                     /locus_tag="O0X12_02890"
     CDS             complement(2891..3358)
                     /locus_tag="O0X12_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HJK53138.1"
                     /translation="MSLNVNDIIPNFCLQDASGREVCMPDPSAKLVVLYFYPKDNTSG
                     CTLEAKEFSELLPEFTKLGVSVYGISQDSVKSHEKFVQKQELTVPLLSDPDHVAIEGF
                     GVWVQKKLYGREYMGVDRSTFIIDGSGKILAVWNKVKAKGHAAAVLQKVQELR"
     gene            complement(3450..4184)
                     /locus_tag="O0X12_02895"
     CDS             complement(3450..4184)
                     /locus_tag="O0X12_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJK53139.1"
                     /translation="MIGPNRVIVRGHGREYYVRAGEGKLSTDLGMIDLVVVAELESGD
                     TIQTHLGQSFTVLVPRPTDFFSHGKRTGAPMMPKDIGMVIAYTGMSRRDRVLDAGTGS
                     GIAAIFFGGVADSVVTCEAREDFSKTAAGNIRDAGLSNVECRACDVLDVSDGPFDVVH
                     LDMMIEPKHVEHAYGLLRCGGYFATYTPFLEQTFCVMDTARRLFGDEAVMTFECMERE
                     LTRSARGTRPSTKVSHTGYLTIARKF"
     gene            complement(4181..4522)
                     /locus_tag="O0X12_02900"
     CDS             complement(4181..4522)
                     /locus_tag="O0X12_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HJK53140.1"
                     /translation="MMPGINPKQMKAAMKKMGMKMEEIEDVQKVVVYTSSGNYVFEPA
                     EVVGITMQGQTSYQLSGTPRFEPAKVEIPESDVELVASQTSVSPEAARAALEECNGDI
                     AEAILKLTAVE"
     gene            complement(4580..>5008)
                     /locus_tag="O0X12_02905"
     CDS             complement(4580..>5008)
                     /locus_tag="O0X12_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HJK53141.1"
                     /translation="DFQFGRGAGDALFPDETTFSYSNTKRIRYANLGKTRLVTVRAGD
                     GRLTLGYAAAERLHAFLPAPKNRVVVMADAVPFILDGKNAMAKHVIASDPDIMAEDEV
                     FVVDENDNLLATGMAILSGTEMLGFSYGTAVKVRQGKNRQ"
BASE COUNT         1312 a         1291 c         1203 g         1202 t
ORIGIN      
        1 cggttggtct gctcggaaaa gatgtgcaga acgagttcgg ctgctgcggc aaaggggatg
       61 gaagcggaac cggctgccgg taatccacac tattttttta cgttcagtcc agtattacta
      121 tcactttgga gagaatcttt tttacgagta aaacacttac aatatgtaag ctgcagtatg
      181 atggatcagg acgcaatagc tcatcttctg gatattctcg gtaacagaaa cagacggaga
      241 atactagatc ttcttcgcca aaagccatgc tttgtaactg agatatctga acgtctggtg
      301 atcaacccga aggcggtaat tgaacacctg gcaatcatgc agaaagagga tgtaatctcc
      361 ttttatcagg atgaaaagcg tcggaaatac tattatctgg tacaggactt tgaactatcc
      421 gtgcagatga caaaacccga acagccgccg cagagcattg ccgcagcatc cgatcaaaca
      481 ggaagtgacg cgtcagcacc acctctcact gacaaaatac tgatgatacg cagactactc
      541 gattcacggg aaaaactgat cgagaacctt gaggcagtgg agaaggacat cgacgagatg
      601 atgaacgatg tcattgtcag tggcagaagt gtgttccaga atagtattga atcagagatt
      661 ctccttgccc tcatctactc cccgctgacc ccgatagaac tctccgatac cattggtcac
      721 ccaatccctg aagtgacagg atcacttcgg acacttgctt caaaaggata tgtagaggcg
      781 gaaggtggtc gttacaggat caagggaaca caaaaggcga ttacacttgc agcaggtcct
      841 gcacttccaa tgaaaatatg agggataact tctatatcca cggactgcaa aaaagatact
      901 gcacgcggta atggtctaat ggcatgacag gggcttccca agcctctaat ccgggttcga
      961 tccccggtta tcgcattcac ttttatggac gagagtcaca attacgcaat cagccttatc
     1021 accgaaaacc aggtaggaat cctccgggac atcggttctg tttgtgcaga gcataatgca
     1081 aacattgtac taacgcagca ggaaaccgta agtcgcggac ctgactccgg ctatgcatgg
     1141 gtcgatctcg agatcactgc atgcggccgc atacaagacc tagtggaagc tcttcaacag
     1201 attcccggag tccgagacgt gaagctgcag gatacattcc gaaccatctt cggcaaacgt
     1261 gtgatcatta tgggaggagg agctcaggtt gcacaggttg ccatgggcgc ggtcaacgaa
     1321 gcagaccgtc ataacatccg gggtgagaga atctcagtgg atacaattcc cctggtaggt
     1381 gaggagaacc ttgcatccgc agtatcggcg gttgcacgcc tgccgcgtgc atcgattctg
     1441 gtcctcgcag gatccattat gggaggagag ataacagaag caatccacgc agtacaggca
     1501 gaaggcattc cggttatctg tctgaagatg gtcggcagtg cctcagccgc agcggatctg
     1561 atagtcaccg atccgattca ggcaggtgtt atggcagtaa tgcacatcag ctccatagga
     1621 gtctttgatc tcaaccgtgt ccgaggaaga gaattttagt tgcaggagat aaattatgac
     1681 aaaacctacc gaatccagca atccgtgctg tagtagtccc tacagctatg aacgtgcaga
     1741 ggtaacgcct gcactggaag cagtgattga gaaagcagta cgcgttcttg cacgtgacgg
     1801 actggtggtg tatcctaccg agaccattta tggacttggg gcagatgcac tgtccgaggt
     1861 tgctgtctat aaggtgtatg aagccaaaca acgccccatg ggaaaaccga tctcagttgc
     1921 agtctcagat attgagatga tctacggcgt cgcttacgtg gacgagttcg ccgagaggtt
     1981 tatccagaaa tttttgccgg gaccggtaac ggtagtatta ccggtgaaaa gctgcctccc
     2041 gcccgatctc tccggtggaa ccggcacgat tggaattcgg tatccggatc atgcggttgc
     2101 acaggcaatc attgcagggc ttgacagtcc aatcacctca acatctgcaa acatctccgg
     2161 tgaagtgtca ccagtgacct ctgaccagat aaatgtcccg cacgattatc tgattgacgg
     2221 aggtaaactt cccggaaccc caagcacagt gattaatctt gccacaaaaa agattgaacg
     2281 ccccggagca atgcttgagg agatcgcctc tttcctgaaa gaggaactct gatctttttt
     2341 ggtatcatca gcccgtattt atctgttggg cacaaagata ctgcatatga cttgcacaga
     2401 ataccgaaaa ccgtctctcc cgatactgat cggatcagtt gttctctgtt ttattgccgc
     2461 tgcacttggg tcactggtaa caattacagg aaccgactcc tggttcatga cagaactcat
     2521 caaacctgag tggcaaccac caagttacct gttcggcccc gtctggacgg ttttgtatct
     2581 tctgatggga attgcacttg ctcttattct ggcacaaggc gcggaacgca gagaggtgcg
     2641 ggttgcggcc ggggtgtttg gagtgcagct tgttctaaat gtactctggt cgtatctgtt
     2701 cttcggctgg cagatgatcg gtgccgcagc agttgagatt atcctgctct ggattgtaat
     2761 aggcgtaaca atgtatctct tctacagaat tcggccagcc gctgcatacc tgctgattcc
     2821 ctatattgca tgggtgacgt ttgcagcggt cctcactgct acaatttgga cgttaaactg
     2881 aactgaggag tcacctcagt tcctgtactt tttgaagaac agcagctgca tgacctttag
     2941 ccttgacttt gttccagaca gcgagaattt ttccagaacc gtcaatgatg aaggtgctgc
     3001 gatcaacgcc catgtactcg cgcccataga gctttttctg aacccacacg ccaaatccct
     3061 caatcgccac gtgatcggga tcggaaagca gcgggacggt cagctcttgc ttttggacaa
     3121 atttttcgtg gcttttcacg ctgtcctggc tgataccgta aacggaaaca ccgagcttgg
     3181 taaactcagg cagaagctcg gagaactctt ttgcctccag cgtacatccc gaagtattgt
     3241 cttttggata aaagtagagc actacaagct tcgcggatgg gtccggcata cagacttcgc
     3301 ggccggaggc gtcctgcagg caaaagttag ggatgatgtc atttacattc agtgacatgc
     3361 tttcaacgta tgcaggagac agacataaag tatcaggttg tggaaagtca gtacaaatac
     3421 cgcacaacat tagcagacaa aaaaaatatt cagaatttcc gtgctattgt cagatagccg
     3481 gtatgagata cctttgtcga tggccgggtt ccccttgcac tccgcgtcag ttcacgttcc
     3541 atacactcaa aggtcattac agcttcgtca ccaaacaacc gccgcgccgt atccatcaca
     3601 caaaatgtct gctccaaaaa cggagtgtac gttgcaaagt acccaccgca gcgaagcaga
     3661 ccataagcat gctccacatg ctttggctcg atcatcatgt cgaggtgcac cacatcaaac
     3721 gggccgtcag agacatccag tacatcacac gcccggcact caacattgga cagccccgca
     3781 tcacgaatat ttcctgcagc cgttttggaa aaatcttcac gtgcctcgca ggtcaccaca
     3841 ctgtctgcaa cgccaccaaa gaagattgcc gcaatcccag aaccagtacc cgcatcaagt
     3901 acgcgatcac ggcgagacat ccccgtatag gcaataacca taccaatatc ctttggcatc
     3961 atcggggcac cggtacgttt tccatgagag aagaagtccg tcggacgcgg taccagaaca
     4021 gtaaatgact gccctagatg ggtctgaata gtgtcgccgc tttcaagctc agcaaccacc
     4081 acaagatcga tcatcccgag atctgttgag agttttccct ctcccgcacg cacatagtac
     4141 tcgcgcccgt gcccccggac aatcactctg tttggtccga tcattctact gccgtcagtt
     4201 tcagaattgc ctcggcaatg tctccgttac actcctcaag agcagcacgg gcagcttccg
     4261 gggacacaga agtctgggag gcgaccagtt ccacatctga ctcggggatc tccacttttg
     4321 caggttcaaa ccggggggta ccggacagct gataggaggt ctgcccctgc attgtaatac
     4381 caacaacctc ggcaggctcg aagacgtaat ttccggagga agtgtagaca acgactttct
     4441 ggacatcttc aatctcctcc atcttcatgc ccatcttctt cattgcagcc ttcatctgtt
     4501 tcggattaat acctggcatc atagttatca accaattctc gttactgttt ttattcaaag
     4561 attcttaatt ccatcgggat tactgccggt ttttgccctg acgtaccttc actgctgttc
     4621 cgtaggagaa gccaagcatc tcggttccag aaaggatcgc cataccggtt gcaagcagat
     4681 tatcattttc atccacaacg aacacttcat cctctgccat aatatccgga tcacttgcaa
     4741 tcacatgttt tgccatcgca ttctttccgt caaggataaa cggcacagca tctgccatca
     4801 ccacaacacg gttctttggg gcagggagaa atgcatgaag acgctccgct gcagcataac
     4861 caagggttag tcgaccatct cccgcacgca cggtgacaag tctggtttta ccaagattcg
     4921 catatcggat acgctttgta tttgagtagg aaaacgttgt ttcatcggga aacagcgcat
     4981 ctcccgcccc cctgccgaac tggaaatc
//
LOCUS       DALTZZ010000156         2058 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_23985,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000156 DALTZZ010000000
VERSION     DALTZZ010000156.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23985"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="O0X12_02910"
     CDS             <1..496
                     /locus_tag="O0X12_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HJK53142.1"
                     /translation="SELKSAGLRVKTDSRDMRPGAKYYHWELHGVPLRVELGPRDLDN
                     NQLVCVNRLGVKTVIPRENAAESVKKLLDEAHDQILERAENHLESHLMTAVTPEECNE
                     KLDGNVVVVHWCGCRDCADKLEELTNSSLLGTEVRSKYVVDDEGPCIICGKKGKAALV
                     GRSY"
     gene            594..1043
                     /locus_tag="O0X12_02915"
     CDS             594..1043
                     /locus_tag="O0X12_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HJK53143.1"
                     /translation="MERTIGFKERRYIEELRILTKATAIDCLIDDRFDRIIYVIKQGD
                     MGLAIGKSGDNIKKMSRVLGKRIEMVEFSEEPDHFIANMFKPAEILDVQFGGDDGPVT
                     VLVPEKSDVGLAIGKGGSTIEKARQLVRRFYCKDIGNIAVPGENEIA"
     gene            1040..1354
                     /gene="hisE"
                     /locus_tag="O0X12_02920"
     CDS             1040..1354
                     /gene="hisE"
                     /locus_tag="O0X12_02920"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HJK53144.1"
                     /translation="MTDAQVFDELWQVICQRATEDPGKKSYVRHLLFHEKGIDKPLEK
                     VGEESCEFVLAVKNGREEAIVGEAADLLFHLMVALKAADVDFAKVEEELEIRRAGMHL
                     HD"
     gene            complement(1369..>2058)
                     /locus_tag="O0X12_02925"
     CDS             complement(1369..>2058)
                     /locus_tag="O0X12_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJK53145.1"
                     /translation="AEAGAKVAVVDIDETEGKKTSDEIIKAGGEACFIHCDVGSESDV
                     LQMIAEVTQTFGTIDILINDAALQLNKGLLDTSTEEFQRVMDVNVTGTFLCTREVAKL
                     MIEQNKGGAIVNFSSTFAVVGSPGYLAYHASKGAIASFTRAAAVALLPYGIRVNAVAP
                     GTTETPGLYDGARDTGNLETGLAGFLALQPLKRFGRPEEIAEVVLMLASDEASFVYGA
                     VWMADGAYTII"
BASE COUNT          519 a          451 c          561 g          527 t
ORIGIN      
        1 gagtgaactg aaatctgccg gtcttcgcgt gaagactgat tcccgtgaca tgcgtcccgg
       61 tgcaaaatat taccactggg aactgcacgg tgtcccgctc cgtgttgagc tcggtccccg
      121 tgatcttgac aacaaccagc ttgtctgtgt gaatcgtctt ggtgtgaaaa cggttattcc
      181 acgtgaaaat gctgcagagt cggtaaagaa actccttgac gaagcccacg atcaaatcct
      241 tgaacgtgcg gaaaatcatc tggaaagcca tctgatgact gctgtaactc ctgaagagtg
      301 taacgagaaa ctcgatggaa acgtagtggt tgttcactgg tgcggctgtc gtgactgtgc
      361 agacaagctc gaggagctga cgaactcaag tctgttagga actgaggtcc gcagcaagta
      421 cgtggtcgat gatgagggcc catgtatcat ctgcggtaag aagggtaaag cggctcttgt
      481 aggaagatcc tactaaccca aaatcattct cgaatactgc ggggcatgtc cccgcatcaa
      541 ttgttgcaat aggtaaattt ctgatacgta tggatattat tgacaaatat gcaatggagc
      601 ggactatcgg atttaaagaa cgccggtata tcgaggagct ccggatttta acaaaggcta
      661 ctgcgattga ctgcctgatt gatgatcgat ttgatcggat catctatgtg attaagcagg
      721 gtgacatggg acttgcaatc ggaaagagcg gtgataatat caaaaagatg tcacgggttc
      781 tcggaaaacg gatcgagatg gtcgagtttt ccgaggagcc ggatcacttt attgccaaca
      841 tgttcaagcc tgctgagatc ctggatgtac agttcggcgg agatgacggt ccggtaacgg
      901 tccttgttcc tgagaaaagt gatgttggtc ttgccatcgg taaaggtgga tcaaccatcg
      961 aaaaggcgcg tcagctggtt cgcagatttt actgtaaaga tatcggaaac attgcagttc
     1021 cgggagaaaa tgagatagca tgacggatgc acaggtgttt gatgagttgt ggcaggttat
     1081 ctgccagcgt gcaacagagg atccgggcaa aaaatcgtat gtccgtcatc ttttgtttca
     1141 tgagaaaggt attgacaaac ctcttgaaaa ggttggtgag gagtcctgtg agtttgttct
     1201 tgcagtgaaa aacggacgtg aggaggcgat tgttggtgag gctgcggatc tgctgttcca
     1261 tttgatggtt gccctcaagg ccgcggacgt ggattttgca aaggtggagg aggagcttga
     1321 aatccggcgt gccggaatgc acctccacga ctaacttttt tttgaggttt agattatagt
     1381 gtaggcaccg tcagccatcc atactgctcc gtagacaaag gatgcctcat cacttgcgag
     1441 cataagtacc acctctgcga tctcttccgg tctgccgaat cttttgagtg gctgaagtgc
     1501 aaggaacccg gcaagccctg tttccagatt accggtatct cgtgcaccgt catagagacc
     1561 gggagtctcg gtagttcccg gtgcaactgc attaacacgg attccgtacg gcaggagtgc
     1621 aacagctgct gcacgggtga atgaggcaat tgctccttta gaagcatggt aagcaaggta
     1681 gcctggggaa ccgactacgg cgaaggttga ggaaaagttg acgatcgcac cgcctttgtt
     1741 ctgttcaatc atgagttttg ctacctcccg ggtacagaga aatgtgccag taacgtttac
     1801 gtccataaca cgctggaact cttccgtgct cgtatccaga agacctttgt tgagctggag
     1861 cgcagcatcg tttatgagga tgtcaatggt cccaaaggtc tgtgtgacct ctgcgatcat
     1921 ctggagaaca tcggattcgg atccaacatc acagtgaatg aaacaggcct ctcctcctgc
     1981 tttgatgatc tcatctgagg tctttttccc ttcggtttca tcaatgtcaa caactgcaac
     2041 ttttgcacca gcttcagc
//
LOCUS       DALTZZ010000157         2080 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_24315,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000157 DALTZZ010000000
VERSION     DALTZZ010000157.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24315"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(127..714)
                     /locus_tag="O0X12_02930"
     CDS             complement(127..714)
                     /locus_tag="O0X12_02930"
                     /inference="COORDINATES: protein motif:HMM:NF016166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF432 domain-containing protein"
                     /protein_id="HJK53146.1"
                     /translation="MLIHPVEPLYVPDAVTDFLEIRFEEIMIEPNGKTVLFLTFPIEI
                     GVFIDSKGKADVIDVFTFKPPKYSLYGASNRGVITRWHQSKVYYYPPQVKNYEEGVLR
                     LTIENSSGDWVYVSRVILYEKGMYVYYDDQVVSMAAEMTIVNRETADVTGVDRPLHED
                     MTRSLRMYRPRRTTSFANIPGAVIDTTFTMDSGLI"
     gene            complement(1092..>2080)
                     /locus_tag="O0X12_02935"
     CDS             complement(1092..>2080)
                     /locus_tag="O0X12_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HJK53147.1"
                     /translation="IQPYQKGLAIRLGTYVGQMNPGFKWVVPFITTVIKLDLRTQVID
                     VPSQEVITKDNSPTDVDAIIYVRVMDPERAYFEVSNYRQATVALAQTSLRGIIGDMEL
                     DEVLYNRDMINRRLRDILDKETDQWGVKIERVEIKEVNPIGAVKQAMTEQTAAERERR
                     AAILRADGEKRAAILKAEGLRQSMILESEGERQSKILRAEGERQSKILQAQGEAQSLR
                     IVALGSRPLDKRAITVLSLNALEKMADGQATKIIFPFEVSSLIKQGAKFLGAEDLVDN
                     DEALQIELDESILGDLPDPEEIAKAVESVKPPESPTADPASADDVPLGGSTR"
BASE COUNT          515 a          520 c          529 g          516 t
ORIGIN      
        1 atcttaatcc cgaagttgag aaacggctgc gtacccagga atcccggatc gtctatctca
       61 ctgaacgggg aacctgtgga attaaacaga ctgtccgcaa taccactgag attgatgtca
      121 gccatttcag atcagccccg agtccatggt aaatgtggtg tcaatgaccg cacctggaat
      181 gttggcaaat gatgtggtcc tccgcggacg gtacatccgc agcgatcggg tcatatcctc
      241 atgcagaggg cgatcaacac cggttacatc agcagtctcc cggttgacaa tggtcatctc
      301 agcagccatg gaaacaacct gatcatcata atagacatac atgcctttct catagaggat
      361 aacgcgtgaa acatataccc agtctccgga tgagttctct atggtcagac gaaggacacc
      421 ttcctcatag tttttgacct gcggaggata gtaatagacc tttgactgat gccagcgggt
      481 aataacaccg cggtttgatg ctccgtacaa cgagtacttc ggcggcttga acgtgaacac
      541 atcaatgaca tcagcctttc ctttcgaatc gatgaagaca ccgatttcaa tcgggaacgt
      601 caggaaaagc acggtctttc catttggctc gatcataatc tcctcaaacc gtatctccag
      661 aaagtccgtg accgcatctg gaacgtacag cggctcaacc ggatgtatga gcattctccc
      721 gttcaaagaa gagatgtttg cctcacgaac ccaactacca agggcacggc gatagtgata
      781 gagtccctgt gtctgctcaa gcgagatgga aagtccggca aagttgacgg cataatcaaa
      841 tgtgaatctg ccgtagttaa aggaagggat agattcggag gatgcatctc ccgtcggcgg
      901 agcagctgac ggaaatttta cgggtgccgg attggggcgg ctgctgcggg taattcgtct
      961 gagtggtatt gtcgttccct ccgtgtctga tgtgatttta ggtaggtggg aaaagtataa
     1021 tgtagtttct attgaacgaa tatacggagc aggcagagga accctctgta aaatgaggcg
     1081 gaaaaaagag attatctggt tgatccacca agcggaacat catccgcaga ggccggatca
     1141 gctgtcgggc tctccggagg tttgacggac tcaacagctt ttgcgatctc ttcgggatca
     1201 ggaagatcgc caagaatact ttcatcaagt tcaatctgca gtgcctcatc attatcaaca
     1261 agatcctcag cgccgaggaa ctttgcaccc tgtttgataa gacttgaaac ctcgaacggg
     1321 aagatgatct ttgttgcctg accatccgcc atcttctcca aagcattcag ggaaagaaca
     1381 gtgattgcac gtttgtccag cgggcgggat ccaagtgcca caatccggag actctgtgcc
     1441 tctccctgcg cctgcaggat tttactctgc cgttcaccct ctgcgcggag aatcttactc
     1501 tgccgttcac cttcagattc aagaatcata ctttgacgca gaccttctgc tttgagaatt
     1561 gccgcacgtt tttctccgtc tgcacggaga atcgccgcac gcctttcacg ttctgctgcg
     1621 gtctgttcag tcatggcctg tttgacagcg ccgatcgggt tgacctcttt gatctcgaca
     1681 cgttcaattt tgacgcccca ctgatcagtt tccttatcga ggatgtcccg cagacgacga
     1741 ttgatcatat cgcggttata gagaacttcg tcgagctcca tgtcgccgat gatgccgcgg
     1801 agacttgtct gcgcaagggc tacggttgcc tggcgatagt tggatacctc aaagtaggca
     1861 cgttcgggat ccataacacg gacgtagata atggcatcca catcggtagg agagttgtct
     1921 ttggtgataa cctcctgtga tggaacatcg ataacctggg ttctgagatc gagtttgatg
     1981 acggttgtga tgaacggtac aacccacttg aaccccgggt tcatctgacc gacatatgtt
     2041 ccgagacgaa tcgcgagacc tttctggtac ggctggatga
//
LOCUS       DALTZZ010000158         2038 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_24631,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000158 DALTZZ010000000
VERSION     DALTZZ010000158.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24631"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="O0X12_02940"
     CDS             <1..467
                     /locus_tag="O0X12_02940"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HJK53148.1"
                     /translation="HDLGKKFSIFFAPDKTDREAKLPNVLPAHLLPEMRLRLVHNADD
                     LREKIFIFRDLLDDRIVETVKDSILREMDARHEEKMPDALYYAQDMFGCEGRSLIFVP
                     KKGEEYLEPIKIPFETYEKIKMLMHGIWERPVEGYTVVDKLWVQSAAKKEQA"
     gene            464..1186
                     /locus_tag="O0X12_02945"
     CDS             464..1186
                     /locus_tag="O0X12_02945"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HJK53149.1"
                     /translation="MITDEDVIICPECDHKQKITICPSVNVTTDPEMREKVLSGELFL
                     FTCEKCGYTGFAGYPFVYEDKETQGGFLIYLEPDCPDREVGIDGDMADQVVYRERPMR
                     LVANLNALKEKIFIFEAGLDDRVMELFKMLTLTKLHGDNPDQVPDVLLFTKVDEDEDG
                     KKIFLAAFRDNKYLGIVELPYSLYQTCVVTGEPIWDTPVTECAAIDQEWIADRLKSGG
                     VEQCSCDTEEDKHTCECDHDRN"
     gene            1173..>2038
                     /gene="argH"
                     /locus_tag="O0X12_02950"
     CDS             1173..>2038
                     /gene="argH"
                     /locus_tag="O0X12_02950"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HJK53150.1"
                     /translation="MTGTDQIRKGRLGGDRSNAVAEFLSSAAADRRIAYCDLRVDMAH
                     ILMLAKQNLIDPASAKTLLSHLHRYMEEGLPEAVFDPAHEDIHAGIEAQLIADCGADV
                     GGRMHLGRSRNDEVATCLRMQTRLDILEILGEIATLRRALIEQAAQHTHSIMPGFTHF
                     QHAQPTTLAHYLLAYESVFARDAGRLFDAYARLNVSPLGSAAFAGTGFALDREATAAY
                     LGFDRAMTNSMDGVASRDFMLEVLAALSILMTNVSRLCEELILWSSAFIRFVELDDAY
                     CSTSSIMPQKKNP"
BASE COUNT          524 a          485 c          549 g          480 t
ORIGIN      
        1 atcatgatct tggcaaaaaa ttctcgatct tttttgcacc ggacaaaaca gaccgtgaag
       61 caaaacttcc aaacgttcta ccggcacatc tgctccccga gatgcgtctg cggttggttc
      121 acaacgcaga tgacctccgg gagaagatct tcatcttccg cgatctgttg gacgaccgga
      181 ttgttgagac cgtgaaggat tccattctgc gcgagatgga tgcgcgccac gaggagaaaa
      241 tgcccgatgc cctgtactat gcacaggata tgtttggctg cgaaggacgt tccctcatct
      301 ttgttccaaa aaaaggagag gaatatcttg aaccgatcaa gattccgttc gagacctatg
      361 aaaagatcaa gatgctgatg catggtatct gggagaggcc ggtcgaaggg tacaccgttg
      421 ttgacaaact ctgggtacag tcagctgcaa agaaggagca ggcatgatca cagatgaaga
      481 tgtgatcatc tgccccgagt gtgatcataa acagaaaatt accatctgcc cgtccgtgaa
      541 tgtaacaaca gatccggaga tgcgtgaaaa ggttctctcc ggggaactgt tcctgttcac
      601 ctgtgagaaa tgcggttaca ccggatttgc cggatatccg tttgtctatg aagacaaaga
      661 aacgcaaggc ggatttctga tctaccttga gccggactgc cccgatcgtg aggtgggcat
      721 tgacggcgac atggcagatc aggttgtgta ccgggaacga ccgatgcggc ttgttgcgaa
      781 tctgaatgcc ttaaaggaaa agatcttcat cttcgaagcg ggccttgacg atcgggtaat
      841 ggaactgttc aagatgctga cactcacaaa actgcacggc gacaaccctg accaggtccc
      901 ggatgttctt ctgtttacca aagtggacga ggatgaggac ggaaagaaga tctttcttgc
      961 agcattcaga gacaacaagt atcttggcat tgttgaactg ccttactcac tctatcagac
     1021 ctgcgtagta accggtgagc cgatctggga tacgccggtc acagagtgtg ctgcaatcga
     1081 tcaggaatgg attgcagacc ggctgaaatc aggcggtgtc gagcaatgca gttgtgatac
     1141 agaagaggac aaacatacct gcgagtgtga tcatgaccgg aactgatcag atccgcaaag
     1201 gacggcttgg aggagatcga agtaatgcag tcgcagagtt tctgtcctca gcagctgctg
     1261 accggcgtat tgcctactgt gatcttcgtg tagacatggc acatattctg atgctggcaa
     1321 agcagaacct gatcgatcct gcttcagcaa aaacgcttct gtcacacctt caccggtaca
     1381 tggaggaagg acttcctgag gctgtgtttg atcctgcaca tgaggacatc cacgcaggaa
     1441 tcgaggcaca gctgatagca gactgcggtg cggatgttgg cggaagaatg catctcgggc
     1501 gcagccgcaa tgatgaggtg gcaacatgcc tgcgaatgca gacacgtctt gacattctgg
     1561 aaattcttgg cgagatcgcc acactgcgtc gtgcattgat tgagcaggca gcacagcata
     1621 cccacagtat tatgcctggc tttacccatt tccagcacgc ccagccgaca acgcttgccc
     1681 actacctgct tgcatacgaa tcagtgtttg ccagggatgc gggacggttg tttgatgcat
     1741 atgcccgcct taatgtatca ccgctcggct ctgctgcatt tgcaggaacc ggttttgctc
     1801 ttgaccggga ggcgacagca gcatatcttg gatttgacag agccatgaca aactcgatgg
     1861 acggagttgc atcccgcgac tttatgctgg aggtacttgc agcactttcg attctgatga
     1921 caaacgtcag tcgtctgtgt gaagagttga tactttggag cagtgcattc attcggtttg
     1981 tagagttgga tgatgcatac tgttcaacca gttccatcat gccgcagaag aaaaatcc
//
LOCUS       DALTZZ010000159         1649 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_25053,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000159 DALTZZ010000000
VERSION     DALTZZ010000159.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25053"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            247..876
                     /gene="cbiM"
                     /locus_tag="O0X12_02955"
                     /pseudo
     CDS             247..876
                     /gene="cbiM"
                     /locus_tag="O0X12_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329798.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt transporter CbiM"
     gene            892..1230
                     /locus_tag="O0X12_02960"
     CDS             892..1230
                     /locus_tag="O0X12_02960"
                     /inference="COORDINATES: protein motif:HMM:NF024588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HJK53151.1"
                     /translation="MPATTFLVVGLIIALVIGCGAVFFASGDPDGLDSTLLVSGGAKD
                     LFSPAHGEIEEVKDPIGWESPMPDYVLGGEDGGTLGGIIALIIGICVALVVILVAARM
                     MYASSDKKSP"
     gene            1279..>1649
                     /locus_tag="O0X12_02965"
     CDS             1279..>1649
                     /locus_tag="O0X12_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJK53152.1"
                     /translation="MHLIETRNLCHAYSTSTEDALKNVHFIADERQRIAVLGPNGAGK
                     STLFRHFNGILMPRSGEVLIRGEPITKANLSEVRRFVGLVFQNPDDQVFSTTVEQDIA
                     FGPYNLGLDADSVAKRVDRVC"
BASE COUNT          371 a          360 c          427 g          491 t
ORIGIN      
        1 acgagatcga tgcagcgatc gatgcactga acacacccga aacccttgaa ctcattacgc
       61 aggcaggtga tatggatcgt ccatcctctc ttcttctcaa actgatgaaa aaaccagaac
      121 ttatcagcac attcggcatt cttgggatga aatcaatgat acggagtatg atcatttaac
      181 gttttgcgaa caattggtaa tacttattac ttgtaaagta ttactatatc gtggaagtga
      241 tgtgacatgc atatccctga ttttctgttt ggatacagtt ccacaggtat tccaattggt
      301 attctctttt ggattattgc actggtgttc attgtactcg cgattaaatg ggcgcgtgac
      361 aatcttgatg aatcaaaaat cccaatcctt gcagtgattg ctgcgggaat ttttgcactg
      421 caggcattca atctcccgac cggttttgga gtgagcgggc atcttgtcgg cggagccctc
      481 gctgcaattg tacttggttc accgtttgcc gctgtgtttg ttctggcaat cgttcttgtg
      541 attcaggcac tggtctttgg tgacggcggt gttcttgcgc ttggagcaaa tatcatcaac
      601 atgggtgtca tcgcagggtt tgtaggtttt tactcgtttt cagctctcaa agagaggatg
      661 ggtactcccg tttctgcagc ggttgccgga tggcttgcat gtgttattgc cgcgggtgcc
      721 tgtgctgttg agattgcggt tcttggtgct gttccaataa gtgtcggcct tcccacgatg
      781 cttttgtatc atgcacttat tggcgtcatt gaagcagtta ttacagggat aattgttgtt
      841 cttatcttta gtgttcgtcc ggaactttcc gggaatgctg agaaaaaagc aatgcctgcc
      901 acaacatttc tggttgtcgg cctgataatt gctcttgtca tcggttgcgg ggctgtcttt
      961 tttgcgtccg gtgatccaga cggacttgac agtactcttc ttgtgagtgg cggcgcaaaa
     1021 gatctcttct cccctgcaca cggtgaaatc gaagaggtta aggatccgat tggatgggaa
     1081 tccccaatgc cggactatgt tctcggcggt gaggatggag gaacgcttgg cggaatcata
     1141 gcactgatta ttggtatttg tgtggcactg gtggtaattc tggtcgcagc acgtatgatg
     1201 tatgcatcgt ctgacaagaa atcaccataa tacggatcgc cacttcttta tctttttttc
     1261 accaaacgaa acataacaat gcacctgatt gaaacccgta acctttgtca tgcatacagc
     1321 acctccacag aggatgcgct gaaaaatgtt catttcattg cagatgagcg gcagcggatt
     1381 gcggtgctcg ggccgaacgg tgccggtaag agtacattgt tccgtcactt caacggtatt
     1441 ctcatgccac ggtccggaga ggtgctgatc cgaggcgagc ccataaccaa agcaaacctc
     1501 tcggaggttc ggagatttgt gggactggtg tttcaaaatc cggatgatca ggtgttttcc
     1561 acaacggtgg aacaggatat tgcatttggt ccgtacaatc tcgggctgga tgcggactct
     1621 gtggcaaagc gggttgatcg tgtctgcca
//
LOCUS       DALTZZ010000160         1974 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_25119,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000160 DALTZZ010000000
VERSION     DALTZZ010000160.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25119"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="O0X12_02970"
     CDS             complement(<1..619)
                     /locus_tag="O0X12_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HJK53153.1"
                     /translation="MSAAPAPGILAIGFAVLAAVLYGISSPVSKVLLDSVPPALLAAL
                     LYLGAGVGMAVVSLVQTSRRQTVREAPLGKKDLPYVIGMIVLDIAAPILLMFGLTMTT
                     AANASLLNNFEIVTTSVIALLVFREAIDRRLWFAILLIVCASIILTVEDMSSFSFSAG
                     SVLVLLACVAWGFENNCTRMLSLKDPVQIVTIKGFGAGGGALLIAL"
     gene            complement(768..1379)
                     /locus_tag="O0X12_02975"
     CDS             complement(768..1379)
                     /locus_tag="O0X12_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53154.1"
                     /translation="MYRDDAVSEVVGFSLILGMVILSLGLLSVHAAPQITEDLEYSQN
                     DNVLRQFAAFKYDLDLFLAAENTGVYGRTVFTFVPEQTSFSVFSFSDLTPGRISMERG
                     VPAFQNNGTDYYPVVFTYQSANRYAENILLELRAGTLTVDPSGLTIPAFGTEDSASSI
                     VVTDSSFVPWVVSGVEQVIVEYRLEHVLHSDSGTLYVFDFSMR"
     gene            complement(1379..>1974)
                     /locus_tag="O0X12_02980"
     CDS             complement(1379..>1974)
                     /locus_tag="O0X12_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53155.1"
                     /translation="AGFFPAGELWEDQISDETAAVQFSDMKLAMDLLWITGDPGDLRS
                     VILDAGSFSVGTSDAVIHVSRNDTAAASYTPLKLVYLPGPAATNRFALAVDSGAVIQM
                     TRETTLMLLPPSVVADRDTVCITVPVLTSPSVEIVKAYPLAVTFYLQNIRENRFDNAT
                     ISVTGDPLLWNALSRELGDAVRYNLTVREVLYRVEVA"
BASE COUNT          589 a          479 c          504 g          402 t
ORIGIN      
        1 caagagcgat cagaagtgct ccgccccctg caccgaaccc tttgatggtt acaatctgga
       61 ccggatcctt taacgagagc atccgcgtac agttgttctc aaaaccccag gcaacgcagg
      121 caagcagaac gagaaccgaa cccgcagaga aggagaaact gctcatatcc tcaacggtga
      181 ggatgatact tgcacagacg atgagaagaa tggcaaacca gagtctccga tcaattgctt
      241 cccggaacac aaggagagcg ataacagatg tggtgacaat ctcaaagttg tttaagaggg
      301 acgcgtttgc cgcagtggtc atggtaagac caaacatcag cagaatggga gcagcaatat
      361 cgagaacaat catcccgatg acgtacggga ggtccttttt tccaagagga gcttcacgga
      421 ccgtctgtct gcgcgatgtc tgaaccagac tgaccaccgc catcccaaca cctgccccaa
      481 gatagagaag agctgcaagc agtgccgggg gcacactgtc aagcagtact ttggagacgg
      541 gggagctgat accgtagaga acagcggcaa gtactgcaaa tccaatggca agaatccccg
      601 gggcaggtgc agcagacata ttctgctaat actatttgca acgattacat ataatcccct
      661 gttagaatgg atttttcaat aattctgcgg atttcttacc aaaaatttca gaacatgcag
      721 cacgtattac gaacgaaaaa aatgtgtgaa aagagtaaac gatttcatca ccgcatgctg
      781 aaatcaaaca catagagcgt gccggaatcg ctatgcagaa cgtgttcgag gcggtactca
      841 acgatcacct gttcgacccc ggagacaacc catggaacaa aggaggagtc agtaacaaca
      901 atggatgatg cagagtcctc cgttccaaat gcaggaatgg taagaccgga cggatctaca
      961 gtgagtgttc ccgcacgtag ttcaaggaga atattttccg cataccggtt ggcagattgg
     1021 taggtgaaga ccaccggata gtaatcagta ccattgtttt gaaatgcagg aacaccacgc
     1081 tccatactga tgcgtccggg agtgagatca gagaaggaaa atacagagaa cgaagtctgt
     1141 tccggtacaa aagtaaagac ggtccggccg taaacaccag tattttctgc tgcaagaaaa
     1201 agatccagat catacttgaa cgctgcaaac tgccggagaa cattatcatt ctgggagtat
     1261 tccagatcct ctgttatctg cggcgcggca tgaaccgaca ggagcccaag ggaaaggata
     1321 accataccaa gaatcaggga aaacccgacc acctctgaaa ctgcatcgtc ccggtacatc
     1381 aggcaacctc aactcgatag agaacttccc ggacggtcag gttgtatctg acagcatcgc
     1441 caagttcccg ggacaatgcg ttccaaagaa gggggtctcc ggttacagag atggttgcgt
     1501 tgtcaaaacg gttttcacgg atgttttgta gataaaaggt aaccgcaaga ggatatgctt
     1561 tgacgatttc caccgacgga gaggtaagta ctggaactgt gatacaaaca gtgtccctgt
     1621 ctgcaacaac ggacggagga agaagcatca acgttgtttc gcgtgtcatc tgaattacag
     1681 ccccgctgtc aaccgcaaga gcaaaccggt ttgtcgctgc gggtcctggc agatatacca
     1741 gtttgagcgg ggtgtaggac gcagccgcag tatcattgcg gctgacatgg atcactgcgt
     1801 ccgaagttcc aacactaaag gatcctgcat cgagaatcac tgaacggaga tctccgggat
     1861 ctccagtaat ccacagaaga tccatggcga gtttcatatc agaaaactgt accgcagcgg
     1921 tttcgtcact gatctgatct tcccagagtt caccggcggg gaaaaaaccc gcgg
//
LOCUS       DALTZZ010000161         2700 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_25236,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000161 DALTZZ010000000
VERSION     DALTZZ010000161.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25236"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(190..1053)
                     /locus_tag="O0X12_02985"
     CDS             complement(190..1053)
                     /locus_tag="O0X12_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53156.1"
                     /translation="MARKKEEFDPLAGPIGGGGIRKKKSKEKTFDVLGGGFGGDHPVE
                     KESFDSYSDVSFGGSSDALMETGGSSSSNAKPKKKGLFASIFGGGRKKKQKKSPAKSQ
                     PLEPETEKPESYVPKHLLDHELEIHDGVMETGIVDRMVDPAYDRRPASQGRYEDPVSD
                     SLGGEVSEEDLFEGEETRPRPVQGLAEKKAAPAPVSADVTYASSSVMEDSHVCYLCGA
                     EFSAALPPFELAPGKYVPVCRTCSHAVKTLKTFRDPSDEREIKLEWYTLCPNLDSDRA
                     DVIIAEARRDS"
     gene            1133..1699
                     /locus_tag="O0X12_02990"
     CDS             1133..1699
                     /locus_tag="O0X12_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJK53157.1"
                     /translation="MSGEDGINNGEYWFNRGENERDCGNLRQAQLCFERAVELDAEVP
                     KYWAELGLTFSECGEHEIAIKCLRRATDLDPKCAESWCGRGVAHCRAGDIDAALFAFD
                     RAVRLNPEISDYWLNLGLMFAELGEHTKAVTAFECGLEINLYDEELWRQKAKCLIALG
                     EDIRARHCYERAGEIEMERATRPDTGNV"
     gene            1696..2061
                     /locus_tag="O0X12_02995"
     CDS             1696..2061
                     /locus_tag="O0X12_02995"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HJK53158.1"
                     /translation="MTSFQEIFLWIGSLYIPILLAVLGAAFWKHPQKTISGNIGYRTR
                     RSRQSQAAWDAAQKLSGKYLCILGIAQMIAVPVVLLLASPVCDPFSLSMICMTAEVLL
                     IAVPVIAVEHYLKIHFPKQ"
     gene            complement(2083..2156)
                     /locus_tag="O0X12_03000"
     tRNA            complement(2083..2156)
                     /locus_tag="O0X12_03000"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2120..2122),aa:Val,seq:tac)
     gene            complement(2234..>2700)
                     /locus_tag="O0X12_03005"
     CDS             complement(2234..>2700)
                     /locus_tag="O0X12_03005"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328025.1"
                     /note="catalyzes the reversible formation of
                     methenyl-H(4)methanopterin from
                     N(5)-formyl-H(4)methanopterin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HJK53159.1"
                     /translation="STQRPATWMEPTSIVGSIQVAGRCVETAVYKLNELGFDTRKILT
                     AAGTAPIPPVRGPKLAMGVTNDATIYHGQINLTMNAPEIVDYLEKIPSCASKGYGKPF
                     NEIFKEAGYDFYKIDTSLFSPAEVVINEVSTGKVYQVGKVDVDVTLKSFGLA"
BASE COUNT          646 a          699 c          699 g          656 t
ORIGIN      
        1 tatcagggac ggaagtgcaa tgtttgtcgc ctctccaata aacggcggca ggaaacatgc
       61 aatcgcaacg gtaaccgcca ccagatactg ctgacggttc agacgcagag aatccatact
      121 aatccattag agagaggtgt gctattaagg tgccgaaatg ataagcaact caaatatgag
      181 taaaaaagat taggagtccc ttctcgcttc ggcaataatc acatcggcac gatccgagtc
      241 gagattcggg caaagtgtat accactccag cttaatctcc cgttcatccg acgggtcgcg
      301 gaaggttttc agcgttttca ccgcatgcga gcaggtccgg cacaccggca cgtattttcc
      361 gggggcaagt tcaaacggag ggagtgctgc ggagaattca gcaccacaga ggtaacacac
      421 atgggagtct tccattacag atgaggaggc gtaggttaca tcagcagaca ctggggcagg
      481 tgccgccttt ttctccgcca atccctgtac cggtcggggc ctggtttcct ctccttcaaa
      541 gagatcctcc tcagacacct ctccacccag ggagtcggag acaggatctt catatcgtcc
      601 ctgagacgct ggtctccgat catatgcagg atcgaccatc cggtcaacaa taccggtctc
      661 cattacaccg tcatgaatct caagttcatg atcaaggaga tgctttggca cgtaagactc
      721 cggcttttca gtctccggct caaggggctg ggatttcgca ggtgattttt tctgtttctt
      781 tttcctgccg ccgccaaata ttgaagcaaa cagccccttc tttttgggtt tggcattcga
      841 tgaagaactg cctccggttt ccatcagtgc atcggatgaa ccgccaaacg atacgtcgct
      901 gtagctgtca aacgactcct tttcaaccgg atgatctccg ccaaatcccc cgccaagtac
      961 atcaaacgtt ttctccttgg acttcttttt ccgtattccg ccgcctccaa tcgggccagc
     1021 aagcggatcg aactcctctt ttttgcgtgc catagtgctt cccaaccggg tctatttcta
     1081 ctttcatatg caacgcccac ctattaatac ctctggagtg atctctgtat agatgagtgg
     1141 tgaagacggg ataaacaacg gggaatactg gtttaaccgc ggagagaacg aacgtgactg
     1201 cggtaacctc cggcaggccc agctgtgttt tgaacgggcg gttgaacttg acgcagaggt
     1261 tcccaagtac tgggctgaac tgggtcttac cttcagcgag tgcggcgaac atgaaattgc
     1321 catcaaatgt ctcagaaggg caacggatct cgatccaaaa tgcgccgaga gctggtgcgg
     1381 cagaggagtt gctcactgcc gggcaggaga cattgatgca gcactattcg catttgaccg
     1441 ggcggtccgg ttaaatccag aaatttctga ttactggctg aatctgggtc tgatgtttgc
     1501 cgagcttggc gagcatacaa aagccgtaac tgcattcgag tgcggacttg agatcaatct
     1561 ttatgacgaa gagctctgga gacagaaggc aaaatgcctc attgcacttg gggaggatat
     1621 ccgggcacgg cactgttacg agcgtgccgg agaaattgag atggaacggg caacacgacc
     1681 ggatacaggg aatgtatgac ctcatttcag gagatttttc tctggatagg atcgctctat
     1741 atcccgatcc ttctcgcagt cctcggtgca gcattctgga aacatcctca aaagaccatc
     1801 tccggaaata tcggctaccg tacccgcaga tcgaggcagt cacaagcggc atgggatgct
     1861 gcacagaaac tttcgggaaa atatctctgt atactcggaa ttgcccagat gattgcggtc
     1921 cctgtcgttc tcctcctcgc aagtccggtc tgtgatccgt tttcgctgtc catgatctgc
     1981 atgaccgccg aggttctgct gatagcagtt ccggtcattg ctgttgaaca ctatctgaag
     2041 atacatttcc ccaaacagtg aaaacaattt ttcaaaaacg agtggacccg aagggattcg
     2101 aacccctgac ctccgccatg taagggcgac gtcataacca cctagaccac gagtccgcta
     2161 atgctctatt ctatagtcgc tgtatcctaa ttaattctgc ggaaatagaa ggggaagaga
     2221 tccccatctt ttcttatgca agaccgaagg acttcagggt cacatcaaca tcgactttac
     2281 cgacctggta gaccttaccg gtggaaacct cgttgatgac gacctctgcc ggggagaaca
     2341 ggctggtgtc gatcttgtag aagtcgtagc ctgcttcctt gaagatctcg ttgaacggct
     2401 tgccgtatcc cttggatgcg cagcttggga tcttctcaag gtagtcaacg atctccggag
     2461 cattcatcgt taagttgatc tgtccgtggt agatcgttgc atcgttggtg acgcccatgg
     2521 caagcttggg gccgcggacc ggcggaattg gggcggtacc tgctgcggta aggatcttgc
     2581 gggtgtcaaa gccgagttcg ttcagcttgt acacggcagt ttccacacag cgtcctgcaa
     2641 cctggatgga accgacgatg gaggtcggtt ccatccaggt tgcaggacgc tgtgtggaaa
//
LOCUS       DALTZZ010000162         3180 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_25309,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000162 DALTZZ010000000
VERSION     DALTZZ010000162.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25309"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="O0X12_03010"
     CDS             complement(<1..268)
                     /locus_tag="O0X12_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HJK53160.1"
                     /translation="MRLAHLFSALEMSDSSWDTALIFGRINMYYLTGTIQDGVLVLRR
                     NGDVLFFVRRSFERAQAESPLGTVYPMKSYRDMRDYLPSDLGRIF"
     gene            complement(414..992)
                     /locus_tag="O0X12_03015"
     CDS             complement(414..992)
                     /locus_tag="O0X12_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HJK53161.1"
                     /translation="MLSDRCLYIIALAVVVVVFFGSCIYGYAESIEMPAMADEIVVTF
                     QSSTTELDSSMPALLSWQIFFNNLKVCLILFIGGVTFGVVTLFVLVSNGYVIGSISSV
                     MLRGYDVSVFAATLVPHGIFEIAAILMAAALGLQTGRSLYLDAQGRDNAGKTCLWYGT
                     RFLLVVVPLLVIAALVESFVTPEIAMLVLSGV"
     gene            complement(992..1849)
                     /locus_tag="O0X12_03020"
     CDS             complement(992..1849)
                     /locus_tag="O0X12_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HJK53162.1"
                     /translation="MFNEIGAFIQKYYIDAVLEDQPYNIYLTITCAILLLVTLYLLYR
                     WARANNIRVDTQLILASMPFVIFGGVLRVVEDTGFIPEPWWVLFVTPPIFFLVLLYTI
                     FVLVLSRTLENHGIVASYTKPYFWVGVISNLACLVFLCWWGITQTELALWVAGCILVM
                     ATVVASLFWALLRYGFSWEYVTHPLYKILIFGQLLDASATSFALDLHPMHYIEQHVLG
                     DALIQLTGTAFVMFPLKMVVLIPAIWVLEKFRNEEGMDEVWHLVLLAMITVGFAPGVR
                     DILRMVLFI"
     gene            1865..2947
                     /locus_tag="O0X12_03025"
     CDS             1865..2947
                     /locus_tag="O0X12_03025"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HJK53163.1"
                     /translation="MNVDNNRILKDKTFDKSRWIQMPRNVVVGHDALLQIPGIIDDLG
                     VAGPIMLLSGPTTMQTVGARVAELLVGHSITTCLVGRITYDEIQRIEDEAKKTGVVLI
                     IAVGGGRVIDTAKVVSYNLDIQFVSVPTAASHDGIASSRASVVTDSGNVSVAAQPPLA
                     IVADTGIIATAPHRLLAAGCADIIANYTAILDWELSHRLTGETISEYALTLSKITAEI
                     LVNNTDLVSHNDETAVWIVVKALFSSGIAMSIAGSSRPASGGEHKFAHMLERLAPGSA
                     LHGEACGLGTIIGMYLHGGDWKGIRTSLKRIGAPTTPQDLGISNEICVEAVLRACEIR
                     PERFTIFDTGITREAAVAAVEALYEV"
     gene            2954..>3180
                     /locus_tag="O0X12_03030"
     CDS             2954..>3180
                     /locus_tag="O0X12_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HJK53164.1"
                     /translation="MDDTEKIVTIVGSVLAHEGAEFVYAGKITECDNCKVGKVCHNAK
                     LREGRRYRVIAVRPTKHECFVHAGGAVAVEVA"
BASE COUNT          942 a          804 c          744 g          690 t
ORIGIN      
        1 caaagattct ccccaaatcc gaggggagat agtccctcat atcgcggtag cttttcattg
       61 gataaacagt accaagcggg gactcagcct gtgcccgctc gaaactcctt cgaacaaaaa
      121 acagcacatc cccgtttctg cgtaaaacaa gaacaccgtc ctgaatcgtt ccagtgagat
      181 agtacatatt gattcttcca aaaatgaggg ctgtgtccca ggacgagtca gacatttcaa
      241 gtgcggagaa gaggtgcgca agccgcatct ccgactcaga tttaggaagt ttcagataca
      301 cgaaaaatct ccatccgtaa aattactgat gtgatatgtt gcaccagaga ttaatactga
      361 gggattaggg agatgtcctc agaaattacc cactctatgg tttgtagacg aaatcagaca
      421 cctgaaagaa cgagcattgc aatctccggc gtcacaaagg actctaccaa tgctgcaatg
      481 accaaaagcg gaacaacaac aagcaaaaaa cgtgtcccat accatagaca tgttttccca
      541 gcattgtctc ttccctgtgc atccagataa agagaacgtc ctgtctgcag accaagtgct
      601 gcagccatga gaattgctgc aatctcaaaa attccgtgag ggacaagtgt tgccgcaaaa
      661 actgatacat catacccgcg cagcataaca cttgagatgc taccgatcac atagccattt
      721 gacaccagaa caaacagggt gacgacccca aacgtcactc caccgataaa caggattaag
      781 cagaccttca gattattgaa aaagatctgc caggacagca atgcaggcat cgaggaatcc
      841 agttctgtag tagagctctg gaatgtcaca acaatctcgt cagccatagc cggcatctcg
      901 atggactcgg catacccata tatacaggag ccaaagaata ccaccacaac aaccgcaagt
      961 gcaatgatat agagacatct atctgacaac atcagatgaa cagcaccatc cgcagaatat
     1021 cccgaacgcc cggtgcaaat ccgaccgtga tcatagcaag cagtaccaga tgccagacct
     1081 catccatacc ctcttcgttc cggaacttct ccagaaccca gatggcaggg atcaggacta
     1141 ccattttgag aggaaacatc acaaatgccg ttcccgtaag ctggataagt gcatctccca
     1201 agacgtgctg ttcaatatag tgcatcggat gcagatccag tgcaaaactt gtggccgacg
     1261 catccagaag ctgaccaaat atcagaatct tgtacagtgg atgtgtcacg tattcccagg
     1321 aaaaaccata gcggagtaag gcccagaaca gagacgccac aaccgttgcc atcaccagaa
     1381 tacatcctgc aacccacagc gcaagctccg tctgagtgat tccccaccag caaaggaaca
     1441 ctaaacacgc aagatttgat atgacaccaa cccagaaata cggttttgta taggaggcga
     1501 caatcccgtg attttccaag gtgcgggaga gtaccagaac gaaaatcgtg tacaacaaca
     1561 ccaggaaaaa gattggaggt gtaacaaaca acacccacca tggctcgggg ataaatccgg
     1621 tatcctccac aacccggagt actccgccga agatgacaaa cggcatcgat gccaagataa
     1681 gctgcgtatc aaccctgatg ttattcgccc gtgcccagcg gtaaagaaga taaagggtga
     1741 cgagtaacaa gattgcacac gtaatcgtca gatagatatt gtacggctgg tcctcaagga
     1801 ctgcatcgat atagtatttt tgtatgaacg cacctatttc attaaacata tgggggtccc
     1861 gaccgtgaac gtagacaata ataggatact aaaggataaa acctttgaca aatcccgttg
     1921 gattcagatg ccacgcaatg tcgtggtcgg ccatgacgca ctactgcaaa tcccaggaat
     1981 tatcgacgac ctcggtgttg caggacccat catgctcctg tcaggcccta caactatgca
     2041 gaccgttggc gcacgggttg cagagctcct agtggggcac tctatcacca cctgccttgt
     2101 tggacgaatc acctatgatg agattcaacg cattgaagac gaggcaaaga aaacaggagt
     2161 cgttctcatc atcgcagtag ggggaggacg tgtcattgac actgcaaaag ttgtttccta
     2221 taatcttgac attcagtttg tcagtgtccc tacggcagca tcccatgatg gtattgcatc
     2281 ttcccgcgct tcggttgtaa cggattctgg gaatgtcagt gttgcagccc aaccaccgct
     2341 tgcaattgta gctgatacag gtatcattgc aaccgccccg caccgactgc ttgctgcagg
     2401 atgtgcagac atcattgcaa attatactgc tattcttgac tgggagctct cccatcgtct
     2461 tacgggtgag acaattagcg agtatgcgct gaccctctca aagatcactg cagagattct
     2521 ggtaaacaac acagatcttg tcagccacaa tgatgaaact gctgtgtgga ttgtagtaaa
     2581 ggcacttttt tcctcaggga ttgccatgag cattgccgga agttcccgcc ctgcatccgg
     2641 cggtgaacac aagtttgccc acatgcttga acgtcttgca ccgggatcag ctttgcacgg
     2701 tgaggcttgt ggactcggta ctatcattgg catgtatctt cacggagggg attggaaagg
     2761 tatccggacc tcactcaaac gcatcggggc cccgaccaca ccacaggacc ttggtattag
     2821 taatgagatc tgcgttgagg cagtcctcag ggcctgtgag atccgacccg aacgattcac
     2881 aatctttgac acaggaatca cgagagaagc agcggttgcg gcagtagaag cattgtatga
     2941 ggtgtaatac agaatggacg atacagaaaa aatagtcacc atcgttggat ccgtgcttgc
     3001 ccacgaggga gccgagttcg tctatgcggg aaagataacc gaatgtgata attgtaaagt
     3061 cggcaaagta tgccacaatg caaaactccg ggaaggacgc agataccgtg tgattgcagt
     3121 tcgtccaaca aaacacgaat gctttgttca tgccggcgga gcagttgcag tggaggtggc
//
LOCUS       DALTZZ010000163         2764 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_25368,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000163 DALTZZ010000000
VERSION     DALTZZ010000163.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25368"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="O0X12_03035"
     CDS             complement(<1..750)
                     /locus_tag="O0X12_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HJK53165.1"
                     /translation="MTKEQWCLRVPVQDGESARRTAIAENILDRTLRPRAENGFLLIP
                     VVSPRDGEFRAEFAENHVMEELPRHEQIGGIVVLQDDDPAGAEKILAARPSAHTALFA
                     TSPVEGEFRTKTFKVLAGVPTTETMYHEYGRRMVIDLTAAYFSARLANERQRILSQMR
                     GDETVLDMFAGVGPFPVMLGGSASFVIANDINPGAVLLMQKNIRLNHLTNVVPMLGDA
                     RNLARILAPMKFDRIIMNLPMDAAEFLPEAAK"
     gene            complement(747..1376)
                     /gene="thiE"
                     /locus_tag="O0X12_03040"
     CDS             complement(747..1376)
                     /gene="thiE"
                     /locus_tag="O0X12_03040"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HJK53166.1"
                     /translation="MYSLYVVTDEKLSRGLTHAEIARRAVAGGANVIQLRDKEKSSRE
                     LYEIACEIREICRDRAIFFVNDRLDIALAAKADGVHLGQDDLPIEAARQIVPENFLIG
                     ISVGNVDEARAAETAGADYVAVSPVFATASKTDAGVGHGIKAIRDIRAAVKCPVIGIG
                     GINADNAAKLIDAGLDGVAVISAVVSAPDVTAAAENLSAIVRNAKEMRR"
     gene            complement(1381..2175)
                     /gene="thiM"
                     /locus_tag="O0X12_03045"
     CDS             complement(1381..2175)
                     /gene="thiM"
                     /locus_tag="O0X12_03045"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HJK53167.1"
                     /translation="MQKYAEILESVRTASPLVHQITNYVTVNDCANITLCIGASPVMS
                     HAPEDVIDMTKIANALVLNIGTLDPGQIEGMLVAGRVAAERNIPIILDPVGAGATPYR
                     TKTAQQLIEELPIAVIKGNAGEIGTLAGVVATVRGVDSGDVAGDKKTIVKNLAAELGC
                     VVIMSGEEDLISNGTRVAGVRNGVPLMGRISGTGCMASAVTGAFAAVAPDTMDGCIAA
                     MVSLGIAGETAANTVAGPGSFKPAFLDAVAALTPEQVAGHAKVTEY"
     gene            2250..>2764
                     /locus_tag="O0X12_03050"
     CDS             2250..>2764
                     /locus_tag="O0X12_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA reductase"
                     /protein_id="HJK53168.1"
                     /translation="MDEQLAKIRSGDLKLYALEKLMPADEAVALRRKYIEEETSTDLT
                     PIGKYTIPIDRVVTRNVENMIGCVQIPVGVAGPVVVNGEYAKGEFWLPLATTEGALIA
                     SINRGCGAIRKAGGAEVRILRDGMTRAPVFAAKSVAHATEVARWAEDNLLLLKVAAEE
                     TTHHGELIGLTT"
BASE COUNT          585 a          718 c          746 g          715 t
ORIGIN      
        1 ctttgctgct tcaggcagaa actccgccgc gtccatcggg agattcataa tgatacggtc
       61 gaacttcatc ggtgcaagaa tcctcgcaag gtttctggca tcaccaagca taggtacaac
      121 attggtcaga tgattcagcc ggatattttt ctgcatcaaa agaacggcac ccggattaat
      181 gtcgtttgcg atcacaaacg atgcggaccc gccaagcatc acagggaatg gtcccactcc
      241 tgcaaacatg tcaagaactg tttcgtcacc ccgcatctgt gataaaattc tctgccgctc
      301 attggccaga cgtgccgaaa agtatgcggc ggtcagatcg attaccatgc gtcgtccgta
      361 ctcgtggtac attgtttcgg ttgttggaac acccgcaaga accttgaacg tttttgtacg
      421 gaactcccct tccactggtg atgtagcaaa aagagctgtg tgtgccgatg ggcgtgcggc
      481 aagaattttt tccgcaccgg ctggatcatc gtcctgcaaa acaacaatgc cgccgatctg
      541 ctcatgccgc ggtagttctt ccatcacatg gttttctgca aactctgcac ggaactctcc
      601 atcacggggg gaaacaaccg gtatcagcag aaatccattt tccgcacggg ggcgaagtgt
      661 ccgatcaaga atgttttctg caattgccgt tcgtcttgct gactcaccat cctgtaccgg
      721 cacgcggaga caccactgct cctttgtcat ctccgcatct cctttgcatt acgcacgatc
      781 gctgaaaggt tctctgccgc tgctgttaca tccggggcac tgaccaccgc ggagataact
      841 gcaactccgt caagtccggc atcaattaat tttgcggcgt tgtctgcgtt gattccaccg
      901 atgccgatca ccggacattt gaccgcggca cgaatatccc tgatcgcctt aataccgtgt
      961 ccaactcctg cgtccgtttt tgaagcagtt gcaaacaccg gactcacagc aacgtagtcc
     1021 gctcccgcag tctctgccgc tcttgcctcg tcaacgtttc ccacagagat tcctatcaaa
     1081 aaattctctg gtacaatctg ccttgccgcc tcaatcggca gatcgtcttg accaagatgc
     1141 acgccgtctg cttttgcggc aagggcaatg tcaaggcgat cgttcacaaa gaatatggca
     1201 cggtcacggc agatttcccg tatttcacag gcgatctcgt acagttctcg cgaggatttt
     1261 tccttgtccc gcaactgaat tacgtttgct cctcctgcaa cagcacggcg tgcgatctcc
     1321 gcatgtgtga gaccgcgtga cagtttttcg tcggtgacta cgtagaggct gtacattggt
     1381 ttagtactcg gtgacttttg cgtgaccggc aacctgttcg ggtgtgagtg cagcgactgc
     1441 gtcgaggaat gccggtttaa atgatcctgg acctgcaaca gtgtttgccg ctgtttctcc
     1501 ggcaatgccg agtgagacca tggcggcaat acagccgtcc attgtgtcgg gtgcaaccgc
     1561 agcaaatgct ccggtaaccg cggacgccat acatccggta ccggagattc ttcccatcag
     1621 gggaacacca ttcctgacac ctgcaacacg ggtcccgttg ctgatcagat cctcttcccc
     1681 gctcatgatt acgacgcacc cgagttcagc tgcaaggttt ttgacgattg ttttcttatc
     1741 tcctgctaca tcgcctgagt ccacgccgcg aaccgttgca acgacacctg cgagggttcc
     1801 gatctctccg gcgtttcctt tgataacggc tattgggagc tcttcaatca gctgctgtgc
     1861 ggtttttgtc cggtacgggg ttgcgcccgc tccgaccggg tcgaggatga ttggaatatt
     1921 tcgttcagcg gcaactctgc ctgcaacgag cataccttct atctgtcctg ggtcgagggt
     1981 accgatgttg agtacgaggg cgtttgcgat ctttgtcatg tcgatgacgt cttcgggggc
     2041 gtgagacata actggggatg ctccgataca gagcgtgatg ttggcacagt cgttgacggt
     2101 gacgtaattc gtgatctggt ggacgagcgg tgatgctgtt ctgacggatt caagaatttc
     2161 tgcgtatttc tgcatggaac acctttttgg taatattagg tctgcggtca gaaaaatgtt
     2221 ttagtgtgcg tgctgaatgg tatattatta tggatgagca gcttgcaaag atccgctccg
     2281 gcgatctgaa actgtacgct ctggagaaac tgatgccggc agatgaggct gtggctctgc
     2341 gcaggaaata tattgaggag gagacttcca ccgatcttac tccgattgga aagtatacta
     2401 ttccgattga tcgtgtggtt acgcggaatg ttgagaatat gatcggttgt gtgcagatcc
     2461 ctgtcggtgt tgcaggaccc gttgttgtta acggcgagta tgcaaagggt gagttctggc
     2521 tgccgcttgc aacgacggaa ggagcactta ttgcgtcgat taaccgaggc tgtggtgcga
     2581 ttcggaaggc tgggggtgcg gaggtgcgga ttctgcgtga cggtatgacg cgagcgccgg
     2641 tgtttgcggc aaagtcggtt gcacatgcta ctgaggttgc ccggtgggca gaggataacc
     2701 tgttgctgct gaaggttgca gctgaggaga caacgcatca tggtgagttg atcggtctta
     2761 cgac
//
LOCUS       DALTZZ010000164         3808 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_25821,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000164 DALTZZ010000000
VERSION     DALTZZ010000164.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3808)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3808)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3808
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25821"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            394..966
                     /locus_tag="O0X12_03055"
     CDS             394..966
                     /locus_tag="O0X12_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53169.1"
                     /translation="MSNRETPNLIAFFAVLIAIGVCIVFFCVAWDQNERSTLPDDPTL
                     SRLGIPDKDGLSIVSHGNEITVDNETAVIELIEQFVQENYDYWFAMGSIQVREAAQNG
                     VAIQIHYNLARELICPPAYDRPNATVLIDRIYVTIPDQPDKKNKLYYYVSPVRDGDLD
                     HTILLPIETANELLKLIGEDPIPQNPLFGL"
     gene            complement(1012..1509)
                     /locus_tag="O0X12_03060"
     CDS             complement(1012..1509)
                     /locus_tag="O0X12_03060"
                     /inference="COORDINATES: protein motif:HMM:NF037716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HJK53170.1"
                     /translation="MICLRPLLLVFVIIILTTAGCIGPEQPGDTQYPMTEMTVSPDSE
                     EESSVLIRISFDDHEVIAELYDNAAANDLRSMLPLTLIFRDYNGAEKIAYPPETLDTE
                     NAPKGYDPEAGDVALYAPWGNIAVFYRDSPYASGLVPLGHVVSGLESLAAVSGEFSAT
                     IDVLE"
     gene            complement(1518..2054)
                     /locus_tag="O0X12_03065"
     CDS             complement(1518..2054)
                     /locus_tag="O0X12_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HJK53171.1"
                     /translation="MKHIVVLSGSPRKGGNSDILCDEFIRGAVDAGNTAEKITISEKK
                     IGYCTACDYCQANGGECIQDDDMTEILEKILACDVLVLATPVYFYSVNAQMKTVIDRV
                     YARYTEISGKEMYYILTAADTSVANMQRTVECFRGFADCLPDAKEKGVIYGTGAWAKG
                     DIKTLPAMQQAYEMGKSV"
     gene            complement(2315..3439)
                     /locus_tag="O0X12_03070"
     CDS             complement(2315..3439)
                     /locus_tag="O0X12_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJK53172.1"
                     /translation="MNGKKLGFGCMRLPLLDENDEASIDIEQTKKMVDVFLERGFTYF
                     DTAWMYCGFRSENAVKEALTDRYPRDRYTIATKLHADFLKSKEDRDAIFYQQLEKTGA
                     QYFDYYLLHDAGRDLYKIYTEMDCFRWLAEKKEQGLIKHIGFSCHDNAEMVDRVFTEH
                     PEMEFVLLQINYLDWENDGIQSHKCYETAVKHGKDVIVMEPVKGGTLARVPEEVERMF
                     KECHPDWSVPSWGIRFAASLEHVKVVLSGMSSLDQMLDNTGYMAEFTPMTEKEIAIVE
                     KAVDLINANITVACTGCSYCTGGCPENIPIPKYFSLYNADLQETKEKIWLPQNQYYAR
                     LAKTFGKASDCIECGQCEQICPQQLPIIQYLKDVAKHFEK"
     gene            complement(3436..>3808)
                     /locus_tag="O0X12_03075"
     CDS             complement(3436..>3808)
                     /locus_tag="O0X12_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HJK53173.1"
                     /translation="FYTDCGKNIHVGKDVFINSGCHFQDQGGIYIGDGTLIGHSVVLA
                     TINHEAEPTMRASMHTKPIVLGKNVWIGSHATILPGVTVGDGAIVAAGAVVTKDVPPG
                     AVVGGVPAKIIKNSVTAGEEK"
BASE COUNT          951 a         1058 c          782 g         1017 t
ORIGIN      
        1 gaacaaaaca agtaccacga gtatgacaat gccgatctcc cgcagcagtt ctctctccat
       61 atttatcagt tgggtcttca gtgttgtacc ggttttgtga tctcccgaca actctcaaat
      121 gagatgaaca cctttttccg ggacctcatg aacgaatgtt ttctcgtgtt ttgttataga
      181 tgtattgacc aaagtgttat accctgccaa ccgatgcaga ttttatttca ccgttttgtt
      241 acgaaacatg aacgggtgta acagtttggc caatctattc atatatgata ccattgatag
      301 atatggatat gaaccgtggg ctccctcatc tcataaatta gcagatatga tctcagttta
      361 tttcattgga gtctcacgag gagacgatct cacatgtcca accgcgaaac ccccaacctc
      421 attgcattct tcgcagttct gattgctatc ggtgtctgca tcgtcttttt ctgtgtagcg
      481 tgggaccaga acgaaagaag tacactccct gatgatccca ctctgtcacg tctgggaatc
      541 ccggacaagg acggcctctc cattgtatcc cacggcaacg aaatcactgt ggacaatgaa
      601 accgccgtca ttgaactgat agaacagttc gttcaggaaa attatgatta ttggtttgcc
      661 atgggcagca tacaagtacg ggaagcagcc caaaacggag ttgcaataca gattcattac
      721 aacctggcac gcgaactaat ctgccctccg gcatacgatc ggccaaacgc caccgttctg
      781 atcgaccgga tctacgtaac cattccggac cagccggata aaaagaataa gctgtactac
      841 tatgtctccc cggttcgcga tggcgatttg gatcacacca ttcttctgcc gatagaaaca
      901 gccaatgaac ttctgaagct gatcggagaa gaccccattc cgcaaaaccc tctgttcggg
      961 ctgtaatccc gaacagagag acactcattt ttcacaatac tgttcttttc gttactccag
     1021 cacgtcaatc gttgcggaaa actccccgct tactgcggca agactttcca gaccggatac
     1081 aacatgtccg agcggaacaa gcccggaggc atacggggaa tcacggtaga agactgcaat
     1141 gtttccccag ggagcataca gtgcaacatc tcccgcttcc ggatcgtatc ctttcggagc
     1201 gttctctgta tccagtgtct ctggaggata ggcaatcttc tctgctccgt tgtagtcccg
     1261 aaatatcagc gtcagcggaa gcattgatcg cagatcgttt gcggccgcat tatcatacag
     1321 ctctgcaatg acttcatgat catcgaagct tatccgtatc agcactgatg actcttcctc
     1381 tgaatccggg gacaccgtca tctccgtcat cgggtactgg gtatcacccg gctgttctgg
     1441 accgatacat ccggcagtag tcaggatgat aatcacaaac accagcagaa gaggacggag
     1501 gcagatcatt gtctcactca gacggacttt cccatctcat acgcctgctg cattgccgga
     1561 agtgttttga tatctccctt tgcccatgca ccggtaccat agatgactcc cttctctttc
     1621 gcatccggca gacagtctgc aaaaccccgg aaacactcaa ccgttctctg catattggca
     1681 accgacgtat ctgccgcggt caggatgtaa tacatctctt taccggagat ctcagtatac
     1741 ctcgcataaa cacgatcgat caccgtcttc atctgggcat tcaccgagta aaagtacacc
     1801 ggagttgcca gaacaagcac atcgcatgca agtatcttct caagaatctc cgtcatatca
     1861 tcatcctgaa tacactcgcc gccgttcgcc tggcagtaat cacacgcagt acaatagccg
     1921 atcttcttct ccgagattgt gatcttttct gcggtgtttc ctgcatcaac cgccccgcgg
     1981 ataaactcat cacaaagaat atccgagtta ccgccttttc ttgggcttcc tgaaagtact
     2041 acaatatgtt tcataacttc tgtttttgtg caaaagtatc cacctaaact ttgaacctgt
     2101 gcctgtatgg ggatactggc aaaaaatggt tctcttatcc ggtgtactgc tgcaccctcc
     2161 ttttctcctg gtatttttgt attctgccaa aatataatcc acagaaacag atctgttcca
     2221 tgcacacagc aatgatacca gctgcatgcc gtacctgacg tgtaaggaaa aaagataatt
     2281 tttttgttgt ctcttcgatt ggttttacca aactctactt ctcaaaatgt tttgcaacgt
     2341 ctttgaggta ctggataatc ggcagctgct gcggacagat ctgctcacac tggccgcact
     2401 caatacagtc gcttgctttt ccgaacgtct tggcaaggcg ggcgtagtac tgattctgcg
     2461 gcagccagat cttctctttc gtctcctgaa ggtccgcatt gtacaaagag aagtacttgg
     2521 ggatgggaat attttccgga catcctcctg tacagtagga acaaccggtg caggcaaccg
     2581 taatgttcgc atttatgaga tcaaccgctt tttccacgat cgcgatctcc ttctcagtca
     2641 tcggcgtgaa ctcagccatg taaccggtgt tgtcaagcat ctgatccagc gaactcatgc
     2701 cgctcaggac cacctttaca tgctccaaac ttgccgcaaa acgaatgccc caggaaggaa
     2761 ccgaccagtc gggatgacac tctttgaaca tcctctcaac ctcttccgga acccttgcaa
     2821 gagtaccgcc tttcaccggc tccatcacga tcacatcctt cccgtgcttt accgcagtct
     2881 cataacattt atgcgactgg attccgtcat tctcccagtc aagataattg atctgcagca
     2941 gtacaaactc catctccgga tgttcggtaa agacgcggtc aaccatctcc gcattgtcgt
     3001 gacaggagaa tccaatgtgt tttatcaggc cctgctcctt cttctccgca agccaccgga
     3061 aacagtccat ctccgtataa atcttgtaaa gatcccgccc cgcatcatgc agaagatagt
     3121 aatcaaagta ctgtgcaccg gtcttttcca gctgctggta aaatatcgca tcccggtcct
     3181 ccttgctttt cagaaaatcc gcgtgcagtt ttgttgcaat cgtgtatctg tcccgcggat
     3241 accggtcggt aagtgcctct tttacagcat tttcactgcg gaacccgcag tacatccatg
     3301 ccgtatcaaa gtaggtaaat ccccgttcga ggaaaacatc aaccatcttc tttgtctgtt
     3361 caatgtcaat actcgcctca tcattttcgt ccagaagagg cagacgcata catccgaatc
     3421 ctaacttctt cccgttcatt tctcttcacc tgctgtcaca gaatttttga tgatctttgc
     3481 cggaaccccg ccgacaactg ctcccggcgg aacatccttc gttaccactg caccggccgc
     3541 aacaatcgca ccgtcgccaa cggtaactcc aggcagaatc gttgcatgag atccaatcca
     3601 gacgttcttt ccaagaacga tcggttttgt gtgcatactt gcccgcatcg ttggctctgc
     3661 ctcatggtta atggtggcaa gaacaaccga atgcccgata agtgttccat ccccgatgta
     3721 gatcccgccc tgatcctgaa aatgacatcc tgagttgatg aacacatctt ttcccacatg
     3781 gatgtttttt ccgcagtccg tataaaac
//
LOCUS       DALTZZ010000165         4336 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_26003,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000165 DALTZZ010000000
VERSION     DALTZZ010000165.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26003"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="O0X12_03080"
     CDS             <1..978
                     /locus_tag="O0X12_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HJK53174.1"
                     /translation="IIPWIGGYATSLFFNMDFYTAIFIGTALTATSIAITANVLREMG
                     KLHQPFAKAIIGAAVIDDIVSLIVLSICKDLGSTGELVPMAVLLTVIKSIGFVVIAAV
                     VGIKVIPPLISKMDTTKLARQFPEFVFIFAMMVAFFYAMMAESVGVSAIVGAFLAGVC
                     VNRVSLKHSLDIKLGAEYLYIIFAAIFFVSLGIIVDLQYLFEKPEMLWFIIALIVVAI
                     ITKVIGCGLPARILGMNTRDSLIIGFGMAPRGEVAMIVGLIALTHFQEMAEAATDVVQ
                     KEYLLQLGNEVFIAIVLMSLVTTVIVPLVYRGWFFKAEKKPDPAVGIQQ"
     gene            complement(1050..2837)
                     /locus_tag="O0X12_03085"
     CDS             complement(1050..2837)
                     /locus_tag="O0X12_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HJK53175.1"
                     /translation="MTPKELNACGITQPDIILVSGDAYVDHPSFAAALIGRALRDAGF
                     TVGIIAQPDWRDHKFKSFTQLGEPRLFFAVVPGAVDPLVNAYTPALKKRSKDSYSPGG
                     KLTRPERTTIVYVNALHRQYPKTPIIIGGIEASLRRFAHYDYWSNSVRQGILADAPAS
                     LLVYGMGELALIEIAKRMRNGDPAERLRDIPGTCWTMSVGEFRKNPLDDAVLLPSYPD
                     VIDKRKFANAYAVMYRDGHKQLVQPHPKTVIIANPPMRPMTTDELDHLYELPFTRRQH
                     PSYQEPVPALEPVQFSLVTHRGCFGGCSFCAITHHQGRIITSRSEESLIREAKRITEM
                     PEFRGTIQDVGGPTANMYGCSCKIWETGTPCPDKNCMKCGKFTNGTERQLALLERLRK
                     LPKVKHVFISSGIRYDLIPKDDQGDRYLLELREHYISGHLKVAPEHISTHVTELMNKP
                     PGRVFSDFYTRFEQLQTRETPKGKNRQYLLPYLMSSHPGCRIEDMIDLALYLQKYHLY
                     TEQVQDFTPVPMTLSTAMYYSGIHPLTQKKVYVPVGGEKRIQRALLQWKDPKQYDLAI
                     AGLMQAKRSDLIGDLIPAAHGRSFKEKKR"
     gene            3362..3994
                     /locus_tag="O0X12_03090"
     CDS             3362..3994
                     /locus_tag="O0X12_03090"
                     /inference="COORDINATES: protein motif:HMM:NF021463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HJK53176.1"
                     /translation="MKQNTFYIIIGIIGLIEVLLFWESIQLSNPFIIGVGFFVGAVLY
                     FVLRRKVTDCYADERQNLIDMKTASATLKAFWVSFFSVNLATVVYVFSASLGFQDFTV
                     SISQQESTNVIPEIGDRLLHGLNVTQQHIDGSVAITIHQNATDVLTSASTTPMEIINI
                     FPAPPETIAISHLGVFGVIQILLLVLMLFIYVAFRIYYAHKFGEWDDDEE"
     gene            3984..4178
                     /locus_tag="O0X12_03095"
     CDS             3984..4178
                     /locus_tag="O0X12_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HJK53177.1"
                     /translation="MKNKIKVYRAMNDLTQEGLAQALGVTRQTILAIEKGKYDPSLEL
                     AFRMARYFNTTIEEIFTFEA"
BASE COUNT         1095 a         1043 c         1050 g         1148 t
ORIGIN      
        1 atcatcccct ggataggagg atatgcaaca tccctgtttt tcaacatgga tttctacacc
       61 gcaatcttca ttggaacagc actcactgca acaagtattg cgattacggc gaacgttttg
      121 cgggagatgg gaaaactgca ccagccgttt gcaaaggcta ttatcggtgc tgcggtgatc
      181 gacgatattg tcagtctgat cgtactgtcc atctgtaaag atcttggttc aaccggcgaa
      241 cttgtgccga tggcagttct tctgacggta attaaatcga tcggatttgt ggtgatcgca
      301 gcagtcgtcg gaatcaaagt aattccgccg cttatctcca agatggatac aaccaaactc
      361 gcccggcagt tcccggagtt cgtgttcatc tttgcaatga tggttgcgtt cttctatgca
      421 atgatggcag agtcagtagg tgtctctgca attgtcggag cattccttgc cggtgtctgt
      481 gtcaatcgcg tgagcctgaa acacagtctt gacatcaaac tcggtgcgga gtatctgtac
      541 atcatctttg cggcaatctt ctttgtgtcg ctgggtatta tcgtggatct tcagtatctg
      601 tttgagaaac cggagatgct ctggtttatc attgcactga tcgtggttgc catcatcaca
      661 aaagtgatcg ggtgcggtct tccggcacgg atacttggca tgaacacccg agactcgctg
      721 atcatcggat ttggcatggc gccacgcgga gaagtggcaa tgatcgtcgg tctgatcgca
      781 ctgacacact tccaggagat ggccgaagcg gcaactgatg tggtccagaa agagtacctc
      841 cttcagctgg gtaacgaggt tttcatcgca attgtgctga tgtcgcttgt tacaactgtg
      901 attgtgccct tggtctatcg gggatggttc ttcaaggccg agaaaaaacc ggatcctgcg
      961 gtgggaatac agcaataata cattttttcc aacagaagtt tgtgacgttg cagtagagtc
     1021 ctactgacaa cctcccgccg agcgttattt tatctcttct tttctttgaa agatcgcccg
     1081 tgcgcagcag gaatcagatc gccaatgaga tcacttcttt tcgcctgcat cagtcctgca
     1141 atggcaagat catactgctt gggatctttc cactgcaaaa gggcacgctg aatacgtttt
     1201 tcgcccccaa ccggaacata gaccttcttc tgcgtaagag ggtgaatgcc gctgtagtac
     1261 attgccgtag acaaagtcat cggaaccggt gtaaagtcct gtacctgctc agtgtaaaga
     1321 tggtactttt gcaggtacaa tgcaagatca atcatatcct caatccggca tccgggatgt
     1381 gaggacatga gatacggcag caaatactga cggttctttc cctttggtgt ttcgcgcgtt
     1441 tgtaactgtt caaaacgcgt ataaaagtca gaaaacacac ggccaggcgg tttgttcatc
     1501 agttcggtca cgtgcgtgga gatgtgttcg ggtgcgacct tgagatgtcc ggagatataa
     1561 tgttcccgta attccagcag gtatcggtct ccctgatcat cttttggaat gagatcatag
     1621 cggatgccgg agctgatgaa cacatgtttg acctttggaa gtttacgcag acgttccaga
     1681 agtgccagct gccgttcggt cccgttggtg aactttccgc atttcataca gtttttgtca
     1741 gggcagggcg ttcccgtctc ccatattttg caggaacaac cgtacatgtt tgcggttggt
     1801 ccgccaacgt cctgaattgt tccccggaac tccggcattt cggttatccg ctttgcttca
     1861 cgaataagcg actcctcgct ccgagaggtg ataatccttc cctgatgatg cgtgatggcg
     1921 cagaacgaac agccgccaaa gcagccgcgg tgagttacca aggagaactg aacaggctca
     1981 agagcgggaa ctggttcctg atacgaggga tgctgcctgc gggtaaacgg gagttcatag
     2041 agatgatcca gttcatcggt ggtcatcggc ctcatgggag ggtttgctat gatcacggtt
     2101 ttcggatgag gctgtactag ttgcttgtgc ccatcacgat acatgactgc ataggcgttt
     2161 gcaaacttcc ttttatctat aacatccgga taggacggga gcagtacggc atcatccagc
     2221 ggatttttgc ggaactcgcc cacactcatt gtccagcagg taccgggaat atcacgaagt
     2281 ctctctgcgg gatctccatt ccgcattcgt tttgcaattt cgatgagagc aagttctccc
     2341 attccataca cgagaagcga tgccggtgca tcagcaagga ttccctgccg gacactgttg
     2401 ctccagtaat catagtgcgc aaaacgccgg aggcttgcct caatgcctcc aatgatgatc
     2461 ggggtcttag gatactgccg gtggagagcg tttacataca caattgttgt ccgctctggg
     2521 cgggtgagtt ttcctccggg tgagtaactg tcctttgatc gttttttcag cgctggcgtg
     2581 tacgcattga ccagaggatc caccgcaccc ggaacgaccg caaaaaacag gcgcggttct
     2641 cccagctgtg taaaactttt gaacttgtga tctctccagt ccggctgggc tatgatccct
     2701 acagtaaaac ccgcatcccg caaagcccga ccgatcaatg ccgccgcaaa cgaaggatga
     2761 tctacgtagg catcaccgga aacgagaatt atatcgggtt gggtaatacc acacgcattc
     2821 agttcctttg gcgtcatcgg taaaaattcc ggctgcggta agggactttg agggagaggc
     2881 atagttgttc tctatgattg taccggaggg gtgataagtg tcgcgtctgt ccttgattac
     2941 gggaaactat atattacata atgtaaaata tatcatacac tgaaggaatg aggaaacaaa
     3001 ggcggtgcct tcaggcacag cagtagtatt ccctttcctc caggaagtga gatgaaaatt
     3061 cctcctctaa tcgtagacac gaaaaacaca ttcagcaaga aaaacaacag cacacaccat
     3121 ccaaacaggt aagaatcagt cacatacaca cccatacata ataccacaca caggtagact
     3181 caaaaagcat cagccggtaa aacccccatg agacggctga tgcaactggg ttctctcttt
     3241 ttctcaagct acaactaccg gatatgagaa aggtataata tccctctaca caattgtata
     3301 atgtaaaaga ttgcatacat catcgtaaaa gatgagggga gtctcggcag ggttgacagt
     3361 catgaaacaa aataccttct atattataat cggcatcatc ggcctcattg aggttctgct
     3421 tttctgggaa tccatccagt tgtccaaccc gtttattatt ggagtgggtt tctttgtcgg
     3481 ggcagtactg tattttgttc tgcgcagaaa agtgacggac tgctatgcag atgagcgtca
     3541 gaatctcatt gatatgaaaa cggcatcagc aacgctgaaa gcgttttggg tcagtttttt
     3601 ttccgtaaat cttgcaacag ttgtctatgt tttcagtgct tctcttggtt ttcaggattt
     3661 tactgtctct atttcccaac aggaaagtac aaacgtcatc ccggagattg gggatcgttt
     3721 actccacgga ctgaacgtta cccagcaaca catagacgga tccgttgcaa ttacaatcca
     3781 ccagaatgca acagatgttc tcacctccgc aagtaccacc ccgatggaga tcatcaatat
     3841 cttcccggca ccgccggaaa cgatcgccat atctcatctc ggcgtttttg gtgttatcca
     3901 gatcctcctg cttgtattga tgctgttcat ctatgtcgca ttcagaattt attacgctca
     3961 taagttcggg gagtgggatg acgatgaaga ataagatcaa agtctatcgg gcaatgaacg
     4021 atctcacaca ggaagggctt gcgcaagctc ttggcgttac ccgccagacg atcctcgcca
     4081 ttgagaaagg gaaatatgat ccttcacttg agcttgcatt tcgaatggca agatacttca
     4141 acacgaccat tgaggagatc ttcacgtttg aggcgtgaag tacttcaatt ctgttttaaa
     4201 aatatacctg caagacacgt tcacatacac gatcgtgccg gcagtcagat aatctaaaaa
     4261 taaaaaaaag tttaggctgc ggccctgcgc cgctcccact catcgagcac ttcctgaccc
     4321 tcaataaata ccagtc
//
LOCUS       DALTZZ010000166         3472 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_26161,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000166 DALTZZ010000000
VERSION     DALTZZ010000166.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26161"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="O0X12_03100"
     CDS             <1..457
                     /locus_tag="O0X12_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HJK53178.1"
                     /translation="GTTAALALLNDQVKKGGVMASSFVGGLSGAFIPVSEDQGMIDAV
                     NRGALTLEKLEAMTCVCSVGLDMIAVPGNTPATTIAGIIADEAAIGMINQKTTAVRLI
                     PVIGKDIGDTVEFGGLLGYAPIQPVNRFSCAAFVNRGGRIPAPIHSFKN"
     gene            complement(501..929)
                     /locus_tag="O0X12_03105"
     CDS             complement(501..929)
                     /locus_tag="O0X12_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53179.1"
                     /translation="MGSDYTPERVFYGNHADPPLMKDFLSAAMYRTADRLLRDGGVPD
                     TAQNGSYGQKLYYCPQCRTLKVGFFCTIIHDDRCWTPQYTCDLCRHPLVLVPNQQNGD
                     DPSRDQMAGPDGAVLRICCKKCGRVYFAPESGCTSVFLWD"
     gene            complement(1235..3301)
                     /locus_tag="O0X12_03110"
     CDS             complement(1235..3301)
                     /locus_tag="O0X12_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HJK53180.1"
                     /translation="MSTHANTKMLSEADGYNLLRRFNVPAPLFEITTSPEAAAAAAAK
                     IGYPVVMKIVSPQIIHKSDAGGVIVGVKTDEEAKTAYNTIISSVKAYNPDAEIKGIIV
                     EEMAKPGLELIIGGKIDPAFGRVITFGLGGTLVEFHKDVGIRILPCNDEEIHSLIKQI
                     KGYKLISGYRGEAPKDEEFLFQTLKNACEFFEKNENVVEFDINPLRLYEKGGCAVDAR
                     VIVQDGPVELPPHYDPSKIVPIDYYKPRSVAVIGASDDKTKMGYAVFHNLLQFPGKVY
                     PVNNKRSEIQGVKCYPTLSAIPGPVDMVAITVPAQLVPGIMEECGQKGVKMAVVITAG
                     FKEMDEDGRALENRMVEIAKSYGIRIVGPNCLGLILPPYKLDTTYVSTSPLPGDLAFI
                     SQSGAIVNAVVGISLSEGSEMGYSEVVSVGNQSDLDFLDYMSYAQRDPHTKAIILYIE
                     EIKNGVAFMEMAKEITKTKPIVAIKAGSSQRGQAAAASHTGSLSGAYEVYMEAFRKCG
                     VTPVKTLPGAFKVAKILSDVTRAPKGRRAVVITNAGGFAVLSNDYAETWGIEIIDLPK
                     ELIDEMNTFLPPFWNKNNPIDLLGDADEARFRGVFDVLCRNPDLWDMAILVNFPNKVL
                     SPEQVARVLIDYSKKTENLLVGTLVGGDCMKPGVSLLTQHNIPVFEELEFTYRTLGHL
                     SWTADR"
BASE COUNT          864 a          877 c          879 g          852 t
ORIGIN      
        1 cggaacgacc gcagcacttg cactgctgaa cgatcaggtg aaaaagggag gagtgatggc
       61 aagttcgttc gttggaggac tttccggggc atttattccg gtcagcgagg atcagggcat
      121 gatcgatgcg gtcaaccgcg gtgcactgac actggaaaaa cttgaggcaa tgacctgtgt
      181 ctgttcagta ggtcttgaca tgattgcagt tccgggtaac actcctgcaa caacgattgc
      241 aggaatcatc gcagatgaag cagcaattgg gatgatcaat cagaaaacaa cagctgttcg
      301 tctgattccg gtcatcggaa aagatattgg tgatacggtg gagttcggcg gtcttctcgg
      361 ctatgcacca attcagccgg tcaaccgctt ctcctgtgcg gcgttcgtga accgcggcgg
      421 aagaattcct gcaccgattc acagcttcaa gaattaattt ctttttttac cctggttttc
      481 tctgaaaaga tcctacagaa ttaatcccag agaaacacgg atgtacagcc gctttccggt
      541 gcaaagtata cgcgtccgca tttcttgcag cagattcgaa gaacggcacc atcaggaccg
      601 gccatctgat cccgggacgg atcatcaccg ttctgctgat ttggcacaag taccagagga
      661 tgtcggcaga gatcacaggt atattgtggt gtccagcacc gatcatcgtg tataattgta
      721 cagaaaaaac ccactttcag ggttctgcac tgcgggcagt agtaaagttt ctgaccataa
      781 ctcccgttct gtgcagtatc cgggacaccg ccgtcccgaa gcagacgatc cgcagtacgg
      841 tacattgccg cggaaagaaa atccttcatc agcggcggat ctgcatgatt cccgtaaaag
      901 accctctctg gtgtgtagtc agaacccata cctagagcaa tatatgtcgc ctctccacag
      961 tcaggacaat gataaacata ccccgaaccc atacacctca cctgtggtat catattgatt
     1021 agcagatgta gtaaaatggt tagatagtac cactaaattg ttgcagacct aaacggatca
     1081 acggtaaaat atctgtactg tccagatgaa acgctgagat atgagaaaaa acaaacggca
     1141 caaaaactcg ttctacagct ttcaggagta gtaacacaaa aaaagaaggt tccagggttt
     1201 cggaggtaaa acccggatat ttggcacagg agggttaacg gtccgcagtc cagcttaagt
     1261 gaccaagtgt tctgtaagta aactccagtt cctcaaacac cgggatattg tgctgggtaa
     1321 gaagcgatac accgggtttc atgcagtctc cacctaccag agttccaacg aggaggttct
     1381 ccgtcttctt cgagtaatcg atcagaacac gggcaacctg ctcggggctt aacaccttgt
     1441 tcgggaagtt gacaagaatt gccatgtccc agagatccgg gttacggcag agcacgtcaa
     1501 acacaccccg gaaccgagct tcatccgcat cgcccagaag atcgatcggg ttgttcttgt
     1561 tccagaacgg cgggaggaaa gtgttcatct catcgatcag ttctttggga aggtcgatga
     1621 tctcaatgcc ccatgtctcg gcgtagtcat tggagagcac cgcaaatcct ccggcgttgg
     1681 tgataaccac cgcacgacgt cccttcgggg cacgggttac atcagagaga atctttgcca
     1741 ccttgaatgc tcccggcagt gtctttaccg gagtcacacc gcacttgcgg aacgcttcca
     1801 tgtacacctc gtaggcaccg gagagagaac cggtatgcga agctgccgcc gcctgtccgc
     1861 gctgggagga accagccttg attgccacga tcggtttggt cttggtgatc tcctttgcca
     1921 tttccatgaa tgccacaccg tttttgatct cttcgatgta gaggatgatg gcctttgtgt
     1981 ggggatctct ctgggcatag ctcatgtagt caaggaagtc aaggtcagac tggttaccaa
     2041 ccgatacaac ctccgagtat cccatctcgg atccttccga gagcgagata ccgacgaccg
     2101 cattgacaat tgctcccgac tgggagatga acgcaagatc tcccggcaga ggagaggtgg
     2161 acacataggt cgtatccagt ttgtacggcg gaaggatcaa accgagacag ttcgggccga
     2221 cgatacggat accgtagctt ttcgcaatct ccaccatccg gttctcaagg gcacgaccgt
     2281 cctcgtccat ctccttgaat cctgcggtga taacgaccgc catctttaca cctttctggc
     2341 cgcactcttc cataataccc ggaacaagct gtgccggaac ggtgattgcc accatgtcaa
     2401 ccggaccggg aatggccgaa agggtcgggt agcatttcac accctgaatc tcgctgcgtt
     2461 tgttgttgac cggatagact ttgccgggga actggagcag gttgtggaac accgcatagc
     2521 ccatcttggt tttgtcatcg gatgcaccga taacggcaac ggaacgcggt ttgtagtagt
     2581 cgatcggcac aatcttggat ggatcatagt gcggaggaag ctcaactgga ccgtcctgca
     2641 caattacgcg tgcgtcaacg gcacagcctc ccttttcata gagacgcaga gggttgatat
     2701 cgaactcaac aacgttctcg ttcttttcaa agaactcgca ggcattcttt aatgtctgga
     2761 agaggaactc ttcgtccttg ggtgcctcgc cgcggtaacc ggagatgagt ttgtatcctt
     2821 tgatctgttt gattaagctg tgaatctctt catcattgca cgggagaata cggatgccga
     2881 catccttgtg gaactcaaca agagtgccgc caagaccaaa cgtaataacg cgaccaaaag
     2941 ccggatcgat ctttccgcca atgatcagct caaggccggg ctttgccatc tcttcaacga
     3001 tgattccctt aatctctgca tcggggttgt aggccttcac cgaggaaata atggtattgt
     3061 atgcggtttt tgcctcttca tccgttttaa caccgacaat cacacctcct gcatcacttt
     3121 tgtgaatgat ctgggggctg acgatcttca taaccaccgg atagccgatc tttgcggcag
     3181 ctgccgcggc ggcttcagga ctggtagtaa tctcgaagag cggggccggc acgttgaatc
     3241 tccgcagaag gttatatccg tctgcttcgg agagcatttt ggtgttggca tgtgttgaca
     3301 tagttctgtc tccattcgtt cacggttcaa gaaccgtgtt tccaccatgg tggaaaggat
     3361 taacattatt tatcatgtag atttattaaa taccctgtgg tctgagggat tgttctgtga
     3421 tcgttaatat gaagttctgc atttgtcgaa aataacaatc cggctttact gc
//
LOCUS       DALTZZ010000167         2364 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_26337,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000167 DALTZZ010000000
VERSION     DALTZZ010000167.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26337"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="O0X12_03115"
     CDS             complement(<1..388)
                     /locus_tag="O0X12_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HJK53181.1"
                     /translation="MRILILGAGAVGLTVAAKLSAHAEVYAVCRKRYADAIAVDGFVM
                     TGIWGEGTYHFPWGECVPSGSWDYIIISTKSAATREICESYQHLFGDAEVVSLQNGIG
                     NEEIIREYTNHVIGAMIITGFEWREGD"
     gene            598..1827
                     /locus_tag="O0X12_03120"
     CDS             598..1827
                     /locus_tag="O0X12_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJK53182.1"
                     /translation="MHKKHLLSVAGLLVVCVALVFAAGCVSDPADSDKTPTVEILYTG
                     AGTMPGLLATGQVDGYIIWQPFVAVGVEGDVGKVVAYSQDLPPEGMWKEHTCCAIGAN
                     SEALKNTDLTTSIVALMILGDKYINDYPDKAAELTADWLFSKQNMVYGNITVNSVDAM
                     NASIPTIMFSSTVTDAWIASNEEFIKSQRELNLITGKLASTTAEESNELLYDFGPYEA
                     ATAQIEAGTLNTPAPVENTISIGYLPSDHDAPLFVLLKDWQYFKDTYNSYLKPTTEST
                     GKVSEAELFINGEKIADVTFVEGSGGPQLMTLLSQNTIQYAVAGTPPYISAIDKSTGS
                     VSLKILAPIQLEGSGLVASMNSPANDWESFVQWVTDRSAAGDNVVIADPQLGSIQDVQ
                     LKAALKDAGITAITMQQ"
     gene            2020..>2364
                     /locus_tag="O0X12_03125"
     CDS             2020..>2364
                     /locus_tag="O0X12_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HJK53183.1"
                     /translation="MATKKSGVYDAILKQINAEMYSAYLYLQFSADASAMKWNGAAAW
                     LRAHASEELTHATRFYDYLVRKGVDVKFDAIEKPATGAKDLLEIFKAVYGHEIKVTAM
                     INKIMETAVAEKD"
BASE COUNT          682 a          628 c          531 g          523 t
ORIGIN      
        1 catccccctc ccgccactca aatccggtga tgatcatagc cccaatcaca tgatttgtat
       61 actcgcggat aatctcctcg ttacctatgc cgttttgcag actcacgacc tcagcatccc
      121 cgaatagatg ctggtaactc tcacagatct cacgcgtcgc cgcagacttt gtggagatga
      181 taatataatc ccatgatccg gatggtacac attcccccca gggaaagtgg taggtcccct
      241 cgccccagat gccggtcata acaaatccgt caacagctat agcatccgca tatcttttcc
      301 ggcagaccgc atacacctcc gcatgcgccg aaagttttgc cgcaacggta agaccaaccg
      361 ccccggcacc gagtatcagt atccgcataa tcgcggaatg atattcgtcc tgcgactaca
      421 ttaagtaaaa ccattggcgc gccagaacag cgcggcaaca tggtttcgga aacgatgttc
      481 tggaacttag acagtataca atacatcact tgtaaacatc atcagtaaca ataactgaac
      541 aaaatatata caatacatta cgatgagtta aatactcttg taagcataca ggaatgtatg
      601 cacaaaaagc atctgctctc agttgcaggt cttcttgtcg tgtgtgtcgc actggttttt
      661 gctgccggat gcgtatcgga tccagcagac tccgataaga ctccgaccgt agagattctc
      721 tacaccggag caggcaccat gccgggactt cttgcaacag gacaagttga cggatacatc
      781 atttggcagc cgttcgttgc tgtcggtgtt gaaggggatg tcggaaaggt tgtagcctac
      841 agccaggatc ttcctccgga aggaatgtgg aaagaacaca cctgctgtgc aattggtgca
      901 aactccgaag cactgaagaa cacagatctc acaaccagca ttgttgctct gatgatcctt
      961 ggtgataagt acatcaacga ctatcccgac aaagccgcag aactgacggc tgactggctg
     1021 ttctccaaac agaacatggt gtacggtaac atcacggtta actcagtcga tgccatgaat
     1081 gcctcgatcc caacgatcat gttctcaagc accgttactg atgcatggat tgcaagcaac
     1141 gaggagttca tcaagtccca gcgtgagctg aatctgatca ccggaaaact tgcatccact
     1201 accgcagagg agtcaaacga gctgctctat gacttcggtc cgtacgaagc agcaactgca
     1261 cagattgaag caggcaccct caacactccc gcaccggtag agaacacaat ctcgatcggc
     1321 tacctcccaa gcgatcacga tgcaccactg tttgttctgc tgaaagactg gcagtacttc
     1381 aaagacacct acaactccta cctcaaaccg accacggaaa gtaccggaaa ggtgtccgaa
     1441 gccgaactgt tcatcaacgg tgagaagatt gctgacgtca cctttgtaga aggttccggc
     1501 ggaccgcagc tgatgaccct gctttcacag aacaccatcc agtacgctgt tgcgggaaca
     1561 ccgccttata tcagtgcaat cgataagagc actggttctg tcagcttaaa gatcctcgct
     1621 ccgattcagc ttgaaggatc cggactcgtg gcaagcatga actccccggc aaatgactgg
     1681 gaaagctttg tacagtgggt caccgaccgc agtgcggcag gcgacaacgt tgtgattgcc
     1741 gacccgcagc ttggatcaat tcaggatgta cagttaaagg ccgcactcaa agatgcaggt
     1801 atcactgcca tcacaatgca gcagtaaaac ggtttgatta caaccgtttc tttttttcaa
     1861 aacattatcc tcggaatatc agatccatct tacatcttga aacaaaagaa ccataaaaga
     1921 tctaaccttg ccaccaccct taggttaata cactcgcgtg aagaatatgg taatatccaa
     1981 caccggaatg aaacgttccg gcaggagatc tgaaagacca tggcaacaaa aaaatcaggc
     2041 gtatatgatg caattttaaa gcagatcaat gcagagatgt actccgcata cctgtacctg
     2101 cagttctccg cagatgcaag tgcaatgaaa tggaatggtg cagcagcatg gctgcgtgca
     2161 cacgcatccg aggaactgac ccacgcaacc cggttctacg actacctcgt ccgcaaagga
     2221 gtcgatgtga agtttgatgc aatcgaaaaa ccggctaccg gtgcaaaaga tcttcttgag
     2281 atcttcaaag cagtctatgg acacgagatc aaagtcactg caatgatcaa caagatcatg
     2341 gaaacggctg ttgccgagaa agac
//
LOCUS       DALTZZ010000168         2122 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_26681,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000168 DALTZZ010000000
VERSION     DALTZZ010000168.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26681"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..>2122
                     /gene="alaS"
                     /locus_tag="O0X12_03130"
     CDS             <1..>2122
                     /gene="alaS"
                     /locus_tag="O0X12_03130"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HJK53184.1"
                     /translation="IRLNDLDNVGRSGRHFTCFEMMAHHAFNTDDHPVYWKDHCVELC
                     SGFVESIGGDLANLTFKENPWFGGGNAGASVEVLMGGLEVATLVFMNLSRKNSGKPQV
                     MIDGKDYYEMPLRIVDTGYGLERFAWASQGTPTAYDAVFPEMIPRLLNAAGMEDRLDN
                     PEFAHILGMNAKFAGLMDIRGEKLHDLRQKVADATGVSLAKLEEMIVPMENIYALCDH
                     TRCLAYMLGDLIVPSNVREGYLARLVLRRSIRMMQEAGVDEDLGDLVVAQMEKIGLSQ
                     FEQEPAIVREIISREVEKYDATIERGTRTVQRVGQTYVKKNQPVPLEELITLYDSHGI
                     PVDLMGRILTETGAQFEIPDDFDSQIADMHSENEGEKPVSPLSKYADRIAGLPATRKS
                     YYERPTDMEFEATVVDVFDDYVILDHTLFYPEGGGQPSDTGMFVTMDAMIRVEEVVKS
                     GDVILHKVRGPGLKRGDRVKGVVDEERRWALMRHHSGTHVILRAAKEVLGPHIHQAGS
                     QLSTDTARLDIRHYTHITPEQLKQIEIVANRLVMENLPVMIKVESRTKAEQKFGFALY
                     QGGVPPGKELRIVQMGAEVQACAGTHCQSTGEIGPIKLLRLEHIQDGVERVEFAAGFA
                     ALEAMQHIQTLLNTSADTLSVQTENLPASVERFFTEWKDQRKEIERLRAKIADLELSR
                     LEGVMVNGTEVVIKQVDVSRKELVT"
BASE COUNT          497 a          508 c          605 g          512 t
ORIGIN      
        1 gtatccggct caatgacctc gataatgttg gtcggtctgg ccgtcatttc acctgctttg
       61 agatgatggc gcatcatgcc ttcaacaccg atgaccaccc ggtttactgg aaggatcact
      121 gtgttgaact gtgcagtggt tttgttgagt ccattggcgg tgatcttgcc aatctcacgt
      181 tcaaggagaa cccgtggttc ggcggaggaa atgcgggtgc aagtgttgag gttctgatgg
      241 gcggacttga ggttgcaacg ctcgttttca tgaacctctc acggaagaac tccggtaaac
      301 cgcaggttat gatcgacggc aaagactact atgagatgcc gcttcggatt gtggataccg
      361 ggtatggtct tgagcggttt gcctgggcat cacagggaac cccgaccgca tatgatgcag
      421 tcttccctga aatgatccca cgtcttctga atgccgcagg aatggaagat cgtcttgaca
      481 acccagagtt tgcccacatc ctcggaatga acgcaaagtt tgccggactc atggacatcc
      541 gcggtgaaaa gctgcatgat ctccggcaga aagttgccga tgcaaccggt gtctcgcttg
      601 cgaaactgga agagatgatt gttccaatgg aaaacatcta tgcactgtgc gatcacaccc
      661 gttgccttgc ctacatgctc ggcgacttaa tcgttccttc caacgtccgc gaaggatatc
      721 ttgcacgtct ggtcctgcgt cgcagtatcc ggatgatgca ggaggcaggc gttgatgagg
      781 atcttggtga tcttgttgtg gcccagatgg agaagattgg tctctctcag tttgagcagg
      841 aaccagcaat tgtacgtgag atcatcagcc gtgaggttga gaagtatgat gcaaccatcg
      901 agcgcggaac ccgtaccgtg cagcgggtcg gtcagaccta tgttaagaaa aatcagccag
      961 tcccgcttga ggagctcatc acgctttatg actctcatgg aattccggtt gatctgatgg
     1021 gcaggatcct gaccgagacc ggtgcacagt tcgagattcc ggatgacttc gactcccaga
     1081 tcgcagatat gcactctgag aatgaaggag aaaaaccggt gtccccactt tccaagtatg
     1141 ctgaccggat tgcaggcctg cccgcaaccc gtaagagcta ctatgagcgg ccgactgaca
     1201 tggagtttga ggctaccgtt gtggacgtct ttgacgatta cgtcattctc gaccacacgt
     1261 tgttctaccc cgaaggagga ggccagccgt ccgataccgg aatgtttgtc acaatggatg
     1321 caatgatccg tgtggaggaa gtcgttaagt caggtgatgt gattctccat aaggttcgcg
     1381 gtcccggtct gaaacgcggt gatcgggtaa agggtgtggt tgatgaggag cgccgctggg
     1441 cccttatgcg tcaccactct ggaacccatg taattcttcg cgcagcaaaa gaggttcttg
     1501 gtcctcacat tcaccaggct ggttctcagt tatccactga tactgccagg cttgatatcc
     1561 ggcattatac ccacatcaca ccggaacagc tcaaacagat tgagattgta gcaaaccgtc
     1621 tggtgatgga aaatctccct gtcatgatca aggtggagag ccgtaccaag gctgagcaga
     1681 agttcggttt tgctctgtat cagggaggag ttccaccagg aaaagagctg cggattgtgc
     1741 agatgggtgc tgaggtgcag gcatgcgcag gaacgcactg tcagagtacc ggtgagatag
     1801 gtcccatcaa gcttttgcgt cttgagcata ttcaggacgg tgtggagcgt gttgagtttg
     1861 cagcaggatt tgccgcactt gaagcaatgc agcacatcca gacgcttctg aatacctctg
     1921 ccgatacgct gtctgtgcag accgaaaacc tcccagcgtc tgttgagcga ttctttaccg
     1981 agtggaagga tcagcgcaag gagattgaac gtctgagggc caagattgca gatctggaac
     2041 tttcgcgatt ggagggtgtt atggtaaacg gcactgaggt tgtcatcaaa caggtggatg
     2101 tctcacgcaa ggaacttgtg ac
//
LOCUS       DALTZZ010000169         1973 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_26714,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000169 DALTZZ010000000
VERSION     DALTZZ010000169.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26714"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="O0X12_03135"
     CDS             <1..264
                     /locus_tag="O0X12_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53185.1"
                     /translation="ILGELIQIEERAQSEVLPDEISATLPFVRVKNPVFDVTPAEYID
                     MIITEAGALPPHLAYTIMREYLGWGFDELQNQFLGTLTGSEFR"
     gene            280..1500
                     /locus_tag="O0X12_03140"
     CDS             280..1500
                     /locus_tag="O0X12_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HJK53186.1"
                     /translation="MDDLIATYYFRPKSGVTADFAAQAISEEQTTGTWTTLSTVNDHT
                     AYVHAYDGEVMSIDPAADGGYLTKIRYPYEIFEPGNIPQYLSVIAGNLFGLGKLEAVR
                     LLDIDIPDALRRHHTGPKFGIEGVRKIVGTEVSRRPHVGTIIKPKVGLTPKDTAAVAY
                     EAAVGGVDLIKDDETLTNQQFCPLMDRLQEVMDALDKAEQETGHTVLYALNITSGGDK
                     IVEIGEQAVKCGANMLMVDVLTAGFSAVQALAADPAITVPIHVHRTMHGALTRNPHHG
                     IAMRPIAKLVRLCGGDQLHTGTVSGKMGGSAAEILGDNTILTEPWDSIAPVFPVASGG
                     LHPGKVHAELATLGTEVVLQAGGGIHGHPDGTRAGAMAMRQAVDAFLAGVSADEYAKT
                     HAELGKALAFWGNT"
BASE COUNT          496 a          451 c          517 g          509 t
ORIGIN      
        1 atccttggcg agctaatcca gattgaggaa cgggctcaga gtgaggtgct cccagacgaa
       61 atttcagcga cacttccgtt tgtccgtgtc aaaaacccgg tctttgatgt gactcctgca
      121 gagtacatcg atatgattat cactgaagcc ggggctctcc cgccgcacct agcctacact
      181 attatgcgtg aatatctcgg atggggattt gatgagttgc agaaccagtt cctgggaaca
      241 ctaaccggtt ctgagttcag ataacaagga gttgatatta tggatgattt aattgcaact
      301 tactacttcc ggcccaagag cggtgtcacc gcggattttg cagcacaggc aatttctgag
      361 gaacagacga ccggtacgtg gaccactctt tctaccgtta atgatcatac cgcctatgtt
      421 catgcctacg atggagaggt gatgagtata gacccagctg ctgatggcgg atatctgaca
      481 aaaatccgct acccttacga gatatttgaa ccaggcaata ttccgcagta tctctctgtc
      541 attgcgggaa atctctttgg tcttggcaaa ttagaggcag tccggctttt ggatattgac
      601 attccggatg cattgcggag acatcatacc ggtccaaagt ttgggatcga gggtgtcagg
      661 aaaatagtgg gaaccgaagt atcacgccgt ccgcatgtgg gtactattat taaaccgaaa
      721 gtgggtttaa caccaaaaga cactgcagcg gttgcctatg aggcagcagt tggcggggtg
      781 gatctgatca aagatgacga aacactgacc aatcagcagt tttgcccgct gatggatcgg
      841 ctgcaggaag tgatggatgc tcttgacaaa gctgaacagg agactgggca tacggttctc
      901 tatgctctta atatcaccag tgggggggac aagatcgttg agattggtga acaggccgtg
      961 aaatgtgggg caaacatgct gatggttgat gttttgaccg ccgggttttc tgcggttcag
     1021 gcactcgctg cggatcctgc aatcacagtt ccgattcacg ttcaccgtac gatgcatggt
     1081 gcactcacca gaaatccgca tcatggtatt gccatgcggc cgatcgcaaa gcttgtccgc
     1141 ctgtgtggag gagatcagct ccatacaggt acggtctccg gtaagatggg aggaagcgct
     1201 gctgagattc tcggtgataa tacgatcctt actgaaccgt gggattcaat cgccccagtc
     1261 tttccggttg catcaggggg tcttcaccca ggcaaggttc atgccgagct tgcaaccctt
     1321 ggtacggagg ttgttcttca ggctggcggc ggcattcatg gtcatccgga cgggacccgc
     1381 gcgggtgcga tggcaatgcg tcaggcggtt gatgccttcc ttgctggtgt ttccgcagat
     1441 gagtatgcca agacccatgc cgagcttgga aaagcccttg cattctgggg caacacctaa
     1501 atcccttttt ttatgcgtct tcccctgtaa tggatcatac cgaatatgta tctgagttat
     1561 ccttccttac aacaattctg tcagttctga tctttaacag gacatattgc ccgcataaaa
     1621 tatgcagggg atgacctcgg tatttgtact gccaaagcca cagcttgttc atgtgcaaaa
     1681 catcatggga agtaccgatg ttaccatctt ttctttgggc accgaatagc tgatttgtga
     1741 tctgttttga ccaatgccac aaatctctta tagaataggc gcagaaaaac ctattaatta
     1801 attttcagga agaattgcgc tcatggatac caggatgaaa tttttagaac acgagcttgc
     1861 tgagcaggaa cgtgaaatga atattaaaca tgaagcaggt gcggaggcag ctccggctcc
     1921 tgtaaaatct gaaaataccg aggctcttga acgaaaagtt cgtgagcttg aag
//
LOCUS       DALTZZ010000170         5907 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_26727,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000170 DALTZZ010000000
VERSION     DALTZZ010000170.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26727"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(94..360)
                     /locus_tag="O0X12_03145"
     CDS             complement(94..360)
                     /locus_tag="O0X12_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HJK53187.1"
                     /translation="MNYEILAKNCSFKEAREIIRENCSEKYEVPAGFKLLDKPLLGIP
                     PILVGIKDEKLVYGYTKPCHGTFVVVVEDPEGMDNVRKNGKPVW"
     gene            complement(357..821)
                     /locus_tag="O0X12_03150"
     CDS             complement(357..821)
                     /locus_tag="O0X12_03150"
                     /inference="COORDINATES: protein motif:HMM:NF020570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 domain-containing protein"
                     /protein_id="HJK53188.1"
                     /translation="MTDEVLLLLGCPQIPVQSPIVLYVADLLQDLGKVPVVAANPSAK
                     QLIVASDPKKHYVAEYRDVDRTIADIADGKVRYPLIVSFIHNDAGLTYTATVAALAPE
                     SLLVTVLFGEHAYDIAAEIKFPAEKIVAPVTHNTRPLLAKIDEVMEWAVLKI"
     gene            complement(892..1776)
                     /gene="fhcD"
                     /locus_tag="O0X12_03155"
     CDS             complement(892..1776)
                     /gene="fhcD"
                     /locus_tag="O0X12_03155"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HJK53189.1"
                     /translation="MEFNGVILEDTYAEGFPVWVARVVITAVTKEWAYKAATEATGFA
                     TSTIGCPCEAGIEKFLPPAETPDNRPGFSILICCSKKALKEQVLERVSECVLTAPTTA
                     VFNGKAGSEEIIPIKLHFFGDGYESKVEVGGIQCWSIPIMGGDFIVEEEIGAVKGVAG
                     GNFLIMGDSQMSALIAAQAAVDAIKGVPGTITPFPGGVVSSGSKVGSKKYKFMGASTN
                     EKFCPTIREKVEETEIPENVKAVYEIVIDGVDEASVMAAMKAGVQAACRVPGVVKITA
                     GNYGGNLGPFKFHLKDCL"
     gene            1916..2827
                     /locus_tag="O0X12_03160"
     CDS             1916..2827
                     /locus_tag="O0X12_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53190.1"
                     /translation="MLSTSPERVKNADTASSVVIGFVLLLVLVAVVLGVWAAVGLPEQ
                     LKTQDEQTLEQLERSFLQYKLAADILRIHEESAADVGIILPNIQGLTYSVTKDTGGRL
                     SIIADDDVGNATVIPIGKVSAEFTGFSGERSAAAYEAGGVHLKTAGSNPAWITPAMLQ
                     ISPVDPTAVQIEINQISFADDTEISGSGHLPVTLSLKEVTTKVLYWKDNATLRFESPD
                     PEQRKLWAAVFFETKERTAKGTVYRTAHPDSFTLLVQTQERIPLFSIRQTDPLTLRIE
                     TSTNTTLSRVVLREAIYETGPAEIIYA"
     gene            2820..3248
                     /locus_tag="O0X12_03165"
     CDS             2820..3248
                     /locus_tag="O0X12_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HJK53191.1"
                     /translation="MHNDDAVTPVVTSVLLLLMVIGCCATIGVIVFHQMNTEETTVPN
                     VRIQTSAESTHYLYHAGGESLFRNDISIYNLDNDITHRTLVEGDKDWTVWKSGEYLYS
                     EQTLSDVYILWRNGEKEYIIYQYGVNRKTGVTPITDVIPD"
     gene            complement(3245..3973)
                     /locus_tag="O0X12_03170"
     CDS             complement(3245..3973)
                     /locus_tag="O0X12_03170"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HJK53192.1"
                     /translation="MPPRRTRFNQDQYALLRRGSELMDFTEWNRWYAKYLADNLFLRS
                     TDAYGAHLAGADLAYWYLEGADLRFAQLQNADLSYSYLKHANLANANLAGTNLWRAFL
                     AGAELSGANPDAAVYDPAGRIKFDPAQESRLRKAAESGDIAAWNAWYRTELSKEDSDV
                     RLYGAYLEEASLRNLDLSGAVLCYAHLEGADLRNVNLSGANLSHVHLEGADLWQADLT
                     NADLRHAELGGANLAGAKKDHALF"
     gene            complement(4046..5212)
                     /locus_tag="O0X12_03175"
     CDS             complement(4046..5212)
                     /locus_tag="O0X12_03175"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJK53193.1"
                     /translation="MTVPEITVYSTKQCMYCNNLKAWLAQNNIPYHSIDVGEDPAAAR
                     EMIDLTGQRGVPVIVIDGEVIVGFDRPRIEALLNIDKGKTIPADHELIIIGSGAAGLA
                     AALYAGRKGLETLVIGGVRGGMITRTADIENYPGFYRISGESLMRLFADQAEDAGAVI
                     LDDVVTDCSVRDGTFVVETLSGRVFTAKSVIAASGRTPKLSGVPGEAELFGKGVSICT
                     ICDGPLFKGKRVAVYGGGNTAAEMALEMSNIAAVVYLISRSPLKADLVTIDRLKEKKN
                     VVIMTGVVITGLHGEGVLTAVETAPRQDLANTTRLPIEGLFLGLGLTPNSVVFSGVVG
                     MNIVGEILVDENCRTDVPGLFAAGDVTSVKGKQLGIAVGDGIKASLAAYEYLRK"
     gene            complement(5304..5804)
                     /locus_tag="O0X12_03180"
     CDS             complement(5304..5804)
                     /locus_tag="O0X12_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HJK53194.1"
                     /translation="MDTKTCIADAFAGESQANRKYKSFSEAAADEGYDHVAKLFRATS
                     AAEEIHARRLLRVGGYIGTTVANLEAGMAGELHETNEMYPSFIKVAEAEGRQDAIITF
                     EHAMKAEAVHADLYKQALEAVKAGRDYEVKTVYLCPVGGNIESNKTSDRCPICGIPGA
                     SFKIVE"
BASE COUNT         1505 a         1554 c         1461 g         1387 t
ORIGIN      
        1 accgcacctc ccgcaagcga aacggagaat gcgtccattg caagaccgac agcgatgaaa
       61 aaagtggaaa gcagtgaggt gaacatgaaa aaactaccag accggttttc cgttctttct
      121 cacattgtcc atgccttccg gatcctctac gacaacgaca aaggttccgt gacagggttt
      181 cgtgtacccg tacaccagtt tctcgtcttt aattccgaca agaatcggcg gaattccaag
      241 cagaggtttg tccagcagct tgaagcccgc cgggacctca tacttttctg aacagttctc
      301 ccgaatgatc tctctcgctt ctttaaaact acagtttttt gcgagaattt catagttcat
      361 attttcaaga cagcccattc catcacctca tcgatttttg caagaagcgg tcgtgtgttg
      421 tgtgttaccg gtgctacaat cttttctgcc gggaacttaa tttctgcggc aatatcatat
      481 gcatgctctc cgaaaagaac agtcaccaga agcgactcag gagcaagggc cgcaacggtt
      541 gccgtatagg tgagtcccgc atcgttgtga ataaaggaaa cgatcagcgg ataacgcacc
      601 ttcccgtcgg cgatatcagc tatggtgcgg tcaacgtcac ggtactctgc cacataatgc
      661 ttcttgggat cacttgccac gatcagctgt tttgccgatg ggtttgccgc gaccaccgga
      721 acttttccga gatcctgcag gagatccgcc acatagagaa caatcggaga ctggaccggt
      781 atctgcggac atcccagcag gagaagcact tcatcggtca tacgtggagt acacgtcagt
      841 gctgaaaagg gataaacatt ggtcttgcgg attacacaaa aaagagagta tttagaggca
      901 gtccttcagg tggaacttga acggaccaag gttgccgccg tagtttcctg cggtgatctt
      961 gacaacaccc ggaactctgc atgctgcctg gacaccagcc ttcatagctg ccatgacgct
     1021 tgcctcgtca acaccgtcga taacgatctc gtagactgcc ttgacattct ccgggatctc
     1081 ggtctcttca accttctcgc ggatggtcgg gcagaacttc tcgttggtgg atgctcccat
     1141 gaacttgtac ttcttggaac caaccttgga accggacgaa acaacgcctc cggggaacgg
     1201 agtgatggtt cccggaacgc ccttaattgc atcgactgct gcctgtgctg caatgagtgc
     1261 ggacatctgg gagtcgccca tgatcaggaa gttaccgcct gcaacaccct tgactgcacc
     1321 gatctcttcc tcaacgatga agtcgccgcc cataattggg atagaccagc actggattcc
     1381 gccaacttcg accttggact catagccgtc accgaagaag tggagcttga tcgggatgat
     1441 ctcttcagaa cctgcctttc cattgaagac tgcagtggtc ggtgcggtca gaacacactc
     1501 ggatacacgc tcaagaacct gttccttgag tgctttcttg ctgcagcaga tcagaatgga
     1561 gaatcccgga cggttgtcag gagtctctgc cggcggcagg aacttctcaa tacctgcttc
     1621 acacgggcat ccgatagtgg aggttgcaaa tccggttgct tcggttgctg ccttgtaagc
     1681 ccattccttc gttactgcag tgattacgac acgggcaacc cacaccggga agccttctgc
     1741 atatgtgtct tcgagaataa caccgttgaa ttccatatgt tcaccagttt ttagtgatag
     1801 tatacaaatg ggacatcaaa gataaagagc attttctttc cacatgatat caccatcggc
     1861 agatcctgag gatcgattca aataatgtca gggaatatag tatcctgttg acacgatgct
     1921 atcaacctct ccggagcggg tgaaaaacgc ggatactgca tcatcggttg tgatagggtt
     1981 tgtcctgctg ctggttcttg tcgccgttgt tcttggtgtc tgggcagcag tagggcttcc
     2041 tgaacaactg aaaacacagg atgaacagac gttagagcag ctggaaagat catttctcca
     2101 gtacaagctt gcagccgata tcctcagaat acacgaagag tccgcagcag atgtcgggat
     2161 cattttgcca aacatacagg gactcacata ctccgtcacg aaagataccg ggggaagact
     2221 cagtatcatc gccgatgatg atgttggaaa tgctactgtg attccgatcg gaaaagtttc
     2281 agcagagttc accggtttct ccggagaacg atcggctgct gcctatgagg caggaggcgt
     2341 acatcttaaa accgcgggca gtaacccggc atggataact ccggcaatgc tgcagatatc
     2401 accagtcgat ccaactgcgg tacaaattga gatcaatcag atctctttcg cggatgatac
     2461 agagatcagc ggcagcggtc atctgccggt gacactaagc ctgaaagagg tcacaacaaa
     2521 agtcctgtac tggaaggaca atgcaacgct ccggttcgaa agtcctgatc ccgaacagcg
     2581 aaagctgtgg gcggcagtgt tctttgaaac aaaagaacgt accgcaaagg gaactgtgta
     2641 tcggacagca catccagatt catttacgct ccttgttcag acacaggaga gaatacctct
     2701 gttcagtatc agacaaaccg atccgcttac actcaggatt gagacgagta caaacacaac
     2761 actatccaga gtagtcctcc gcgaggcaat ctatgagaca ggacctgcag agatcattta
     2821 tgcataacga cgatgctgtt acacctgtag tgacatcagt tctcctgctt ctcatggtta
     2881 tcggatgctg tgcaacgatt ggcgtcatcg tttttcacca gatgaacaca gaggagacca
     2941 ctgttccgaa tgtaaggatc cagacatcag ccgaaagtac ccactacctg taccatgcgg
     3001 gaggagaatc gctgttccgc aatgacattt ccatctataa tcttgacaat gacattaccc
     3061 atcggaccct ggtagaagga gataaggact ggaccgtatg gaaaagcgga gagtatctct
     3121 acagtgaaca gaccctctcg gatgtgtaca ttctctggcg caatggggag aaggagtata
     3181 tcatctatca gtacggcgta aacagaaaga ccggtgttac cccgatcacc gatgtcatcc
     3241 ccgattaaaa caatgcgtga tcctttttcg cgcccgcaag gtttgcaccg ccaagctctg
     3301 cgtgccggag atctgcgttc gtaagatctg cctgccagag atcggcaccc tccagatgca
     3361 catgggataa attcgctccg gaaagattca cgttccggag atcggcacct tccagatgtg
     3421 cataacacag tactgcaccc gagagatcca gattgcgcag tgatgcttcc tcaagatatg
     3481 ccccgtacag ccgcacatcg ctgtcttctt tggaaagctc cgtccggtac cacgcattcc
     3541 atgccgcaat gtctccggac tccgcagctt tacggagacg tgattcctgt gccggatcaa
     3601 acttgattct gcccgcagga tcatacactg cagcgtcagg gtttgcaccg gagagttcag
     3661 cacctgcaag gaatgcccgc cagagatttg ttccggcaag atttgcattt gccagattgg
     3721 catgttttag ataggagtag gagagatcgg cattctgcag ctgggcaaac cgaagatcgg
     3781 caccttccag ataccagtac gcaagatccg ctcctgcaag atgtgcacca taggcgtcgg
     3841 tactgcggag aaagaggtta tctgcaagat actttgcata ccagcggttc cactccgtga
     3901 aatccatcaa ctcagaaccg cggcggagca acgcatactg atcctgatta aaccgggtgc
     3961 gacgtggcgg cataattcgg gcagacctcg ttttgtactg ttatgtgcgg gtactgtata
     4021 agaacatgtc cctggttaat gagatctact ttctcagata ctcataggcg gcaagcgatg
     4081 ctttgatacc gtctcccact gcaataccaa gctgtttgcc cttaacagag gtgacatccc
     4141 ctgcggcaaa cagccctggg acatctgttc tgcagttttc atcaacaagg atctcaccaa
     4201 cgatgttcat tccgacaaca cccgagaaga ccacagagtt cggcgtaaga ccaagtccga
     4261 ggaagagacc ctcgattgga agacgggttg tgtttgccag atcctgacgc ggtgcagtct
     4321 cgacggcggt aagtactccc tcaccgtgaa gaccggtgat tacaactccg gtcataatca
     4381 ccacattctt cttctcttta agacgatcaa tggtgaccaa atccgccttc aggggactgc
     4441 gggagatcag atacacgacc gctgcgatgt tactcatctc aagagccatc tccgcggcgg
     4501 tgtttccgcc cccgtacacc gcaacccgtt ttcctttgaa aagcggcccg tcacatatag
     4561 tacagatgga aactcctttt ccaaacagct ccgcctcacc cggtacaccg ctgagcttcg
     4621 gagttctgcc ggatgcagca atcacggatt ttgcagtgaa cacacgacca gagagtgttt
     4681 caacaacgaa cgttccgtca cgcacggaac agtccgtaac cacatcatcc aaaataacag
     4741 ccccagcatc ctctgcctga tctgcaaaca gacgcatcag agactctccc gatatccggt
     4801 aaaaaccggg atagttctca atatcagcgg ttcgtgtaat cattccgccg cggacacccc
     4861 cgataaccag tgtctcaaga ccttttctcc cggcgtacag tgcagctgca agtcccgctg
     4921 cacccgagcc gataataatc agttcgtgat ctgcagggat ggttttgcct ttgtcaatgt
     4981 tgagcagtgc ctcaattctc gggcgatcga atcccacaat cacctcgccg tcaataacga
     5041 tcaccggcac cccacgctga ccggtaagat caatcatctc tcgggcggca gcaggatcct
     5101 cacctacatc gatcgagtga taggggatat tgttctgcgc aagccatgcc ttcagattat
     5161 tacagtacat acactgtttg gtggagtata ccgtaatctc gggaacagtc atactccaac
     5221 atagggcgcg tgataattaa aatgatagga taggagcacg gattggagag atatgtggaa
     5281 aaagtgaaaa atggaaagtt ggattactcg acgatcttga aggatgcgcc gggaatgccg
     5341 cagatcgggc agcggtcaga ggtcttgttg ctctcaatgt ttccgccaac cgggcagagg
     5401 tacacggtct taacctcata gtcacggcca gccttgactg cctcaagcgc ctgcttgtag
     5461 agatctgcgt gaacagcctc tgctttcatg gcgtgctcaa aggtaatgat tgcatcctga
     5521 cggccttctg cctcggcaac tttgatgaag gaggggtaca tctcgttggt ctcgtgcagt
     5581 tcgcctgcca taccggcctc aaggtttgca acggtcgttc cgatgtaacc accgacacgg
     5641 agaaggcggc gtgcatggat ctcctcagct gcggaggttg cgcggaaaag ctttgcaacg
     5701 tggtcgtatc cctcatcggc tgctgcttcg gagaaggatt tgtacttacg gtttgcctgt
     5761 gactcaccgg caaaagcatc tgcaatgcat gtcttagtgt ccatgcaata ttatttgcag
     5821 aagttgggga ataaaggttt ggtatgggta cccgtcgagg gaaaaattcc aaaagttctt
     5881 gaaaaaaata ctgccgagat cggcgac
//
LOCUS       DALTZZ010000171         2402 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_26787,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000171 DALTZZ010000000
VERSION     DALTZZ010000171.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26787"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="O0X12_03185"
     CDS             <1..626
                     /locus_tag="O0X12_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HJK53195.1"
                     /translation="IPVAVRFCRDSWERLGRGAAPVTVSVNPSYASNVGECLGSVLFP
                     DIPKTVDRLCAAFPDADADAVITEWQKAEEQMYYVCDKYLRKYTPPVVAVAAQEGYVV
                     CAKRMMERYFGSDVPVVFARNRTAAEIPYSINSAEINAAVREAEPDLILGSTFEAAAC
                     RKAAFFGITPPDRSRVSVAARPFAGVEGGLMLIEGALNALMNRQKGT"
     gene            complement(672..1802)
                     /locus_tag="O0X12_03190"
     CDS             complement(672..1802)
                     /locus_tag="O0X12_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HJK53196.1"
                     /translation="MRSVSETIAAVAPSATMAMSNRSKEMIASGIDVISLAVGEPDFA
                     TPAHITQAAIDALNRGETHYAPSRGLPELTQAVAEKLNRDNHIAATQKQIMVTAGAKD
                     AIRITMMATLNKGDEVVVIDPSWVSYEPCVQIAGGKAVHYSLNADFQVDESIYDVITD
                     KTKMIIVNTPSNPTGSVLGRSSLRLIADVCTDHDLFCLSDEIYEKLVYGKEHVSIATI
                     GDMLDRTITINGFSKAYAMTGWRLGYLAAPLDVIPAMDKVMQHSVGCVNTFAMWGGVA
                     ALTGDQTCVEEMRKQFEQRRSFVIGRLAGMGLSTAPAEGAFYAFINVGGDDVATANKW
                     LDKAHVAATPGTAFGAPGWIRISYAASMERLAEALDRIEAVQ"
     gene            complement(1799..2218)
                     /gene="ribH"
                     /locus_tag="O0X12_03195"
     CDS             complement(1799..2218)
                     /gene="ribH"
                     /locus_tag="O0X12_03195"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HJK53197.1"
                     /translation="MTDKPVRLGFVVAEFNRDLTYMMEMEAEEHAKFLGASVSDRIYV
                     PGAYDMPLAIRKLLKKSDIDAVVTIGCVIEGATDHDQIVVQHAARKIIDLSLEFDKPV
                     ALGISGPGMTRLEAEDRIEYGKRAVESAVKMVRRLAE"
BASE COUNT          554 a          639 c          640 g          569 t
ORIGIN      
        1 gcattccagt tgcagtgcgg ttctgccgtg attcctggga acggctcggg cgcggtgcag
       61 caccagttac ggtttcagta aatccgtcct atgcttcaaa cgtaggagag tgtcttggca
      121 gtgtgctgtt tcccgacatt ccaaaaacgg ttgaccggct gtgtgcagcg tttccggatg
      181 cggatgcgga tgcggtgata accgaatggc agaaggcgga ggagcagatg tattatgtat
      241 gtgataagta tctgcgaaag tacacaccgc cggttgtggc agttgcggca caggaaggat
      301 acgtggtgtg tgcgaagagg atgatggagc ggtacttcgg gtcggatgtt ccggttgtgt
      361 ttgcccgcaa ccgtacggca gcggagatcc cgtactcgat aaactcggct gagatcaatg
      421 cggcggtgcg tgaagcagag ccggatctta tccttggttc aacgtttgag gcggcagcat
      481 gcagaaaggc ggcatttttc ggcattactc caccggacag aagccgtgtg tcggttgccg
      541 cccgaccgtt tgccggtgtg gagggggggc tgatgctgat agaaggggca ctgaatgcac
      601 tgatgaaccg gcagaaagga acctgagata caggtttttg gaaatatgct gttttaaaaa
      661 aaggaaacgg gttactgtac cgcttcgatc cggtcaagtg cttctgcaag ccgttccatt
      721 gatgccgcat acgagatacg gatccagccc ggtgcaccaa atgcagttcc cggagttgcc
      781 gcaacgtgtg ccttgtccag ccacttgttt gccgttgcca catcgtcccc gcccacattg
      841 atgaacgcat agaatgcacc ctcagccgga gcagtggaaa gtcccatgcc tgcaagacga
      901 ccgataacaa aacttctgcg ctgctcaaac tgtttgcgca tctcctcaac acaggtctga
      961 tcaccggtca gcgcagcaac gcctccccac atggcaaaag tattcacgca gccgaccgaa
     1021 tgctgcatta ctttgtccat tgccggaatc acatcaagcg gtgctgcaag ataaccgagc
     1081 cgccatcccg tcattgcgta tgctttggag aagccgttga tggtgatggt ccgatccagc
     1141 atatcaccga tcgttgcaat cgaaacatgc tctttcccat agacgagctt ctcatagatc
     1201 tcatcggaaa gacagaacag atcatgatcc gtacacacat ccgcaatcag ccggagtgat
     1261 gatctgccaa gaaccgaacc cgttggattg gacggcgtgt tgacgatgat catctttgtt
     1321 ttgtccgtga tcacgtcata gatcgactcg tccacctgaa aatccgcgtt cagcgagtag
     1381 tggaccgcct ttcctccggc aatctgcaca cacggctcgt acgaaaccca ggacggatcg
     1441 atgaccacga cctcgtctcc cttgttcagc gttgccatca ttgtaatacg gatggcatcc
     1501 tttgcaccag ccgttaccat gatctgtttt tgcgttgccg caatgtggtt gtcacggttc
     1561 agtttctcgg caaccgcctg cgtcagttca ggaagtccgc gggacggagc atagtgcgtc
     1621 tcaccgcggt ttaacgcatc aattgccgcc tgggtgatgt gagcgggcgt tgcaaagtcg
     1681 ggctcaccca ccgcaagact gatcacatca atacctgatg cgatcatctc cttggaacgg
     1741 ttgctcattg ccatcgttgc cgatggtgca accgctgcaa tcgtctccga aaccgacctc
     1801 attctgcaag tctccggacc atcttcaccg ctgactcaac tgcacgcttt ccgtactcga
     1861 tacggtcttc agcctcaagt ctcgtcatgc cgggtccgga gataccaagc gcaaccggtt
     1921 tgtcaaactc aagggaaaga tcgatgatct tccgggctgc atgctggaca acaatctgat
     1981 cgtgatccgt tgcaccctca ataacacagc cgatggtgac aactgcatcg atgtccgact
     2041 ttttcagaag tttgcgaatg gcaaggggca tatcataggc gcccggcaca tagatccggt
     2101 ccgaaactga cgcaccaagg aactttgcgt gctcctctgc ctccatctcc atcatatagg
     2161 taagatcgcg gttaaactct gcaacaacga atccgagtct gactggtttg tcggtcatga
     2221 taatctcagt acaattctgt cgcaaaagta tatagtggtt cacaaaaacg tcacaactca
     2281 aaaatgagtt gacaacatct cagcgggatt tgtgatcgtg cgtgtggtga aggtccggtg
     2341 cgtgcggatg actgtgttcc aggatttcat gcgtatgcat gtgcgaatgt tcacccgtta
     2401 cc
//
LOCUS       DALTZZ010000172         6983 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_26964,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000172 DALTZZ010000000
VERSION     DALTZZ010000172.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26964"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(111..428)
                     /locus_tag="O0X12_03200"
     CDS             complement(111..428)
                     /locus_tag="O0X12_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2551 domain-containing protein"
                     /protein_id="HJK53198.1"
                     /translation="MLSPSDLRKEIENRLRKYLARDKSGIRKELLSLFVRAKSLTVAQ
                     IHEKLAEKFSVSYHSVASMVGTIASKLGILSTRRNPSGTLGVYEVKEQYVDVIEQAIA
                     TAV"
     gene            533..1135
                     /locus_tag="O0X12_03205"
     CDS             533..1135
                     /locus_tag="O0X12_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HJK53199.1"
                     /translation="MLYPLYEYLVRRKLAPDLFPKEICFMISEVDLLADPGRLEMIAG
                     WCMEFSTIKRIIFHIASKDPAALEGKMPSLQSFGGSASVRLSMPSGETTFGSGTPEIL
                     IVLGRSGREEITEAIAAIAREGVNPETITEETIESHLLYQVNPDFVIKTGGSHLTDFL
                     IWQSVYSELFFTDINWCRFRRLDLLRALRDYQSRVRRYGQ"
     gene            1125..2021
                     /locus_tag="O0X12_03210"
     CDS             1125..2021
                     /locus_tag="O0X12_03210"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HJK53200.1"
                     /translation="MGSEMIISASRRTDIPAWFSEWFCNRLCEGFCYVRNPYSQKITR
                     VSLMREDTDVIVFWTKNAEPMMQRLSAVDASGIPYYFQYTVTPYGSDVEPGLTKRDCI
                     KNFLELSEMLGRDRVLWRYDPVLITEKYSVEFHADAFAKLCELFSEATCRCTISFVDA
                     YRTSPFRTCTFDEMIRLAEIFSATAKRFGLPLVSCAEEIDLDRFGISHASCVDKEICE
                     SLCGRPLPVKRDRNQRTSCGCVESIDIGAYGTCMCGCRYCYACGRRTVAAHDPASPLI
                     SGWPMDNEPVFSRSSGQTTLFR"
     gene            complement(2014..2781)
                     /locus_tag="O0X12_03215"
     CDS             complement(2014..2781)
                     /locus_tag="O0X12_03215"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HJK53201.1"
                     /translation="MNDLLPLAVPAVAVLLTFLYASWQDIKTRTVATATWYPAAVVGG
                     VVVLYFWHLQFQHPTNTAISVLVLSVVICILMWGCAKAGLFGLADAKAMILLAATVPV
                     NPFAVWLFPSLALSTLVNAGVIALLVPAALLIRNTIRKEHAPFWLMCSGMQVAGDSLT
                     RYFGFVAEEITDGDPISRRFLPAASSIRALKENSACSIRNLREHPERFEKELELYRKA
                     GRVWITYGIPFMIPITIGYILALAGFSLPDAALSLFI"
     gene            complement(2855..3232)
                     /locus_tag="O0X12_03220"
     CDS             complement(2855..3232)
                     /locus_tag="O0X12_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HJK53202.1"
                     /translation="MTEKTHTCGCHNGTGDMKELEKNIGHVPVFFRDLAESDPGMHEA
                     VLKLDNYIWADGALSRKQKKLMAIAIAAAMRDDHALHAQMQGAKKLGVTIEEVDEALR
                     VAFMLAGMPSYVYGKTEAAEIFK"
     gene            3423..4031
                     /locus_tag="O0X12_03225"
     CDS             3423..4031
                     /locus_tag="O0X12_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HJK53203.1"
                     /translation="MTKKVLALFGSNILGGNTDKLLSAAIKGVEDAGCTVDRINVAHI
                     HAGPCNQTYSCMIEPTCAIEDDMQDAYRKIKQADSIIIATPVMTYGIPGALKSFMDRC
                     QPFYMAKYYRKQPFITPEHAKIRRTLFLCIGGMNAPDVFTGPVLTTKAFCAIVDAKYA
                     DELLQNDMDTIVNLDNKPEILAEAYEKGKALGERIVKMRDGE"
     gene            complement(4040..4516)
                     /locus_tag="O0X12_03230"
     CDS             complement(4040..4516)
                     /locus_tag="O0X12_03230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJK53204.1"
                     /translation="MTAACVKAEGKNMECVIKHFSELSRDELFEIYKLRVAVFVVEQQ
                     SPYPEVDDADPVAYHIWFKDQEGILAYARVLPAHTVCETVSIGRIIAVKRRCGLGTKI
                     VAEAIRVAEEMFGAETITITAQTYAKEFYEKSGFVQCSEEYLDAGVPHIKMIRKRK"
     gene            complement(4491..5252)
                     /gene="uppS"
                     /locus_tag="O0X12_03235"
     CDS             complement(4491..5252)
                     /gene="uppS"
                     /locus_tag="O0X12_03235"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HJK53205.1"
                     /translation="MSIRSVAENLYAKRLRKSLMYIPNHVAIIQDGNRRYAKAHGKDT
                     GYGHRLGADTTERVLDWMGELGIRHVTFFAFSTENFRRPEEEKEELHQLFMEKFSALL
                     TDERVSQYGINVSVIGDRSLLDAELLQIIEKAEAATKNNSRYFVHIALAYGGRNEIVA
                     TARKIVAAVRDGSISADAITPRLVTENMYQGISMPPVDLIIRTANDRRTSNFLPWMAN
                     GNEAAVCFCTPTWPEFRYVDMLRALRVYDCRMRES"
     gene            complement(5249..6259)
                     /locus_tag="O0X12_03240"
     CDS             complement(5249..6259)
                     /locus_tag="O0X12_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HJK53206.1"
                     /translation="MAKAKDKLPKNLSEGCRLCYQGAKLVLFITGRCDRDCWYCPLSE
                     ERKNVDVIFANDEKITKPEEAIPEAEVMDALGTGVTGGEPLLELDRVVEFCTYLKNHF
                     GPNHHIHLYTGHAPTEEELAALAPCVDEIRMHPPHEEWSTIMDSKFPESIANAKKMGF
                     SVGIEVPSLPGLRHFFPLLDSLDFLNINQLEWGDSCADAMRERKLEPENPLCNAIKGS
                     TKWAGEINGHPKVHYCTSTFKDSVQLRERLKRIARNSARPFDMITEDGTVMYGSMDPG
                     DDLEMILSCLKAGSCYEEMPDGTIEFDWQQMRKVPRKFGGERKIIERYPNDGMIVEVI
                     PL"
     gene            complement(6262..>6983)
                     /gene="lysS"
                     /locus_tag="O0X12_03245"
     CDS             complement(6262..>6983)
                     /gene="lysS"
                     /locus_tag="O0X12_03245"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HJK53207.1"
                     /translation="PVTYEWISLKGRGEMHSSKGVVLTIRDMLDIVPPEVLRYMIAKT
                     KPDKTIAFDPGMGLLRLIDEYDRAAASGDSREYELSVIASPQTTIPFRHMVTVVQIAQ
                     NDEGIFDILKRSGYEIVDRNAVLDQANRARMWLERYAPEDVKFAIQPTLPEIAAAEPE
                     NVKAAVAAYATAIAAGEWKADVLHNAVYDAAASAGITGKELFTAIYRAFLGADRGPRL
                     GWFLEAIGREATLARLNEMTA"
BASE COUNT         1600 a         1764 c         1789 g         1830 t
ORIGIN      
        1 ggcgatcact tcatcagtga cggtgatggg agaaaaggga cggggcccgt cctgttgcat
       61 gtccattctg tattaattgg gccttgcccg tcaaaaaagt taagggaggg ctagaccgct
      121 gttgcgatcg cctgctcaat tacatcaaca tactgttctt tgacttcgta taccccgaga
      181 gttccggagg ggttgcggcg tgtagagaga attccgagtt tagaggcgat tgtgccaacc
      241 attgatgcaa ccgaatggta actgacactg aacttctccg ccagtttctc gtgaatctgc
      301 gcgaccgtca gtgattttgc acggacaaac aggctgagca gctctttgcg gattcctgat
      361 ttatcgcggg caagatactt tcgaagccgg ttctctatct cttttcgcag gtcagaagga
      421 gatagcatgt ctcttagttt attttgaata tataaataga tatcgttaat tcctgaaatt
      481 atccggaaaa agagcggatt catatgttct gaaatgcaaa atgtgtacta agatgctcta
      541 cccgctgtat gaatatctgg tcaggcgcaa actggctcct gatctgtttc caaaggagat
      601 ctgctttatg ataagcgaag tggatcttct tgcagatccc ggcagactgg aaatgattgc
      661 agggtggtgc atggagtttt ccacaatcaa acggattata tttcatattg catcaaagga
      721 tccggcagca cttgagggaa agatgccgtc tttgcagtct ttcggaggtt cggcctcagt
      781 ccgcctgagt atgccgtcgg gagagactac gtttggttcg gggacccctg agatcctgat
      841 cgttctcggg cgcagcgggc gcgaggagat cacagaggca attgccgcga tcgcacggga
      901 aggagttaat cctgagacaa tcacggagga gacgatcgag tcacatcttt tgtatcaggt
      961 gaatcctgac tttgtcatca agaccggcgg cagccacctg acggattttc tgatctggca
     1021 gtcggtgtac tctgagctgt ttttcacgga tatcaactgg tgcaggttcc gccgtctgga
     1081 tcttctgcgg gcactccgtg attaccagtc acgggttcgc cggtatgggc agtgagatga
     1141 tcatctctgc atcccggcgt acggacattc ctgcatggtt ttcggagtgg ttctgtaacc
     1201 gtctttgcga agggttttgt tacgtccgca acccctattc acagaagatc acacgtgtat
     1261 ccctgatgcg tgaggacacg gacgtgatcg tgttctggac aaaaaatgca gagccgatga
     1321 tgcaaagact ttctgcggtg gatgcatccg gaattcccta ctattttcag tacaccgtaa
     1381 cgccctacgg ttctgatgta gaacccggtt taacgaagcg tgactgtatc aaaaattttc
     1441 tcgagctttc ggaaatgctt ggaagagatc gtgtgctgtg gcgttacgat ccggttctta
     1501 tcaccgaaaa atactcggta gagtttcatg cagatgcgtt tgcaaagctg tgtgaactat
     1561 tttcggaggc aacctgtcgc tgtacaatca gttttgtgga tgcgtatcgc acctctccgt
     1621 ttcgtacctg cacttttgac gagatgatcc ggcttgccga gatcttttct gcaacggcaa
     1681 aacggtttgg tctgccgctt gtctcctgtg ctgaggagat cgatctggac agatttggta
     1741 ttagccatgc atcctgcgtg gacaaagaga tctgtgagtc tctctgcggc cgaccgcttc
     1801 cagtaaagcg tgatcgaaac cagcgtactt cctgcggctg tgttgagagt attgatatcg
     1861 gggcatacgg tacctgcatg tgcggatgcc ggtactgtta tgcctgcgga cgcaggactg
     1921 ttgcggcaca tgaccccgca tccccgctga tttccggctg gccaatggac aatgaaccgg
     1981 ttttttcgcg tagttctggt cagacaactc tctttagatg aagagggaga gagcggcgtc
     2041 cggcagactg aatccggcga gtgccagaat atagccgatg gttatcggaa tcatgaacgg
     2101 aattccgtag gtgatccaga ctctccctgc cttccggtag agctccaatt ctttttcaaa
     2161 tctttctgga tgttcacgga ggtttcgaat tgaacaggct gagttttcct tcagggcacg
     2221 aatcgaactt gcagcaggaa gaaatctgcg ggagatagga tccccgtcgg tgatctcttc
     2281 tgcaacaaaa ccgaagtacc gggtaagcga gtcacctgcc acctgcatgc cggaacacat
     2341 gagccagaac ggggcgtgtt ctttgcggat ggtgtttcgg atcagcagtg ctgcgggaac
     2401 gagaagtgcg ataacgcctg cattaacgag ggttgagaga gcaagcgagg gaaacaacca
     2461 tacggcaaac ggattgacgg gtacggttgc ggcgaggagg atcattgctt ttgcatccgc
     2521 cagaccgaac agaccagctt ttgcacatcc ccacatcagt atgcagataa caactgagag
     2581 tacaagtacg cttattgcgg tgtttgtcgg gtgctgaaac tgcaggtgcc agaagtacag
     2641 gactaccact ccgccgacaa ctgcggcggg gtaccaggtc gcggttgcta cagtccgtgt
     2701 tttgatgtcc tgccatgatg cgtaaagaaa tgttaggaga accgcgactg cggggactgc
     2761 aagcggcagg agatcattca tactatctgt ttaggacgga aaagatgatg aaagtttaga
     2821 gggggaggtg tgcaggctcc cgcaaaaatc tggtttattt gaagatctct gctgcttcgg
     2881 tcttgccgta cacataagac ggcatacctg cgagcatgaa tgcgacacgc agtgcctcgt
     2941 caacctcttc aatggtaacg ccgagtttct ttgctccctg catctgtgcg tggagtgcat
     3001 gatcgtcacg cattgcagct gcaatggcga ttgccatcag tttcttctgt ttgcgggaga
     3061 gtgccccgtc cgcccagatg tagttgtcaa gtttgagaac tgcttcatgc attccaggat
     3121 ccgactcggc aagatcacgg aagaataccg gaacatggcc gatgttcttt tccagttctt
     3181 tcatgtcgcc ggtcccgttg tggcagccgc aggtatgagt tttttctgtc atggatattt
     3241 tcactgatcc aatatatcag ccggttttgc tattagtctt ttaggtggtg acatcggaga
     3301 taaaatcctg tgggatctcc cacccgggag tttccaggtt tgttgtctgt tcttgatggc
     3361 ttttcccggt aatttggacc catcacctca tttttatatt tggctgacac ataaaccacg
     3421 taatgacaaa aaaagtgctg gcgctgttcg ggagcaatat cctcggagga aacaccgaca
     3481 aactgctgag tgcagcaatc aaaggtgtag aggatgccgg ttgtacggtt gaccggatca
     3541 atgttgccca tatccatgcc ggaccctgta accagacgta ctcctgtatg attgaaccga
     3601 cctgtgctat tgaggatgat atgcaggatg cctaccgcaa aatcaaacag gcggacagca
     3661 ttatcatcgc aaccccggtg atgacctacg gcattccggg ggcattaaag tcctttatgg
     3721 atcgctgcca gccgttctac atggcaaagt actatcgtaa acagccgttt atcacgccag
     3781 aacacgcaaa gatccgccgc acactctttc tgtgtatcgg aggaatgaat gcgcccgacg
     3841 tctttaccgg tccggtcctg accacgaaag cattctgcgc aattgtggat gcaaaatacg
     3901 cggacgaact gctgcaaaac gatatggaca ccatcgtcaa tcttgataac aaaccggaga
     3961 ttcttgctga ggcatacgaa aaaggaaaag cgctcggtga acgtattgta aaaatgcgtg
     4021 acggagagta atccctctct tactttctct tccgtatcat tttgatatgc ggaacccctg
     4081 catcaagata ttcttcggag cactgtacaa atcctgattt ctcgtaaaat tcctttgcat
     4141 aggtctgtgc agtgatcgtt attgtttctg ctccaaacat ttcctccgcc accctgatcg
     4201 cttcggcaac tatcttcgta cccagcccgc atctgcgttt aactgcaatt atcctcccaa
     4261 tagataccgt ctcgcacact gtatgtgcag gcagaacccg tgcatatgcc agaatccctt
     4321 cctgatcttt aaaccagata tggtaggcaa ccggatccgc atcgtccacc tcaggatacg
     4381 gagactgttg ttccacgaca aaaaccgcaa ccctgagttt gtagatctca aatagttcgt
     4441 cgcgggacaa ctccgagaaa tgtttgatca cacactccat gtttttccct tcagctttca
     4501 cgcatgcggc agtcatacac acgaagagca cgcagcatat ccacgtaccg gaactccggc
     4561 catgtcggcg tacagaagca gaccgccgcc tcgttgccgt ttgccatcca cggtaaaaag
     4621 ttcgaggtcc ggcgatcgtt tgccgtacgg ataattagat ctacaggtgg catagaaatt
     4681 ccctgataca tattttccgt taccagtctg ggtgttattg catccgcgga gatcgatcca
     4741 tcccgaacgg cagctacaat ttttctcgca gtcgccacaa tctcgttgcg gcctccgtat
     4801 gcaagagcaa tgtgtacaaa gtagcgggaa ttattctttg ttgctgcctc tgccttctca
     4861 attatctgaa gaagttccgc atccagcagt gatcgatcgc ctatcaccga aacattgatc
     4921 ccgtactggg atacccgctc atccgttaga agagcagaga acttttccat aaacagttgg
     4981 tgcagttcct ctttttcctc ttcaggacgg cggaagttct cggttgaaaa tgcaaaaaaa
     5041 gtcacatggc ggatgccaag ttctcccatc cagtcaagca cacgttctgt tgtgtcggca
     5101 ccgagtctgt gtccgtatcc cgtatctttt ccatgggctt tggcatacct tcggtttccg
     5161 tcctgaatta ttgccacatg gtttgggatg tacatgagac tctttctgag tctttttgca
     5221 tacagattct ctgcgaccga ccggatgctc acagcgggat cacctcaacg atcattccgt
     5281 catttggata tcgctcgatg atctttctct ctccgccgaa cttccgggga actttacgca
     5341 tctgctgcca gtcaaactcg atcgttccgt ccggcatctc ctcgtagcaa cttccggctt
     5401 tcagacagga gaggatcatc tccagatcgt ctccgggatc catggatccg tacatcaccg
     5461 ttccgtcttc ggtgatcata tcaaacgggc gtgcagagtt tcgtgcaatc cgtttcagcc
     5521 gttcacggag ctgtaccgag tctttgaatg tcgaggtaca gtaatgtact tttgggtgcc
     5581 cgttgatctc gcctgcccac tttgttgatc ctttaatcgc attgcaaagg ggattttccg
     5641 gttcgagttt tcgttcccgc atcgcatcag cacaggagtc accccactcc agctggttga
     5701 tgttcagaaa gtcaaggctg tcaagcagcg ggaagaaatg acgcagtccc ggaagtgagg
     5761 ggacttcaat cccaacagaa aatcccatct tctttgcatt tgcgatcgat tcgggaaact
     5821 tggagtccat aatggtactc cactcctcgt gcggcggatg catgcgaatc tcatcaacac
     5881 acggagcaag ggctgcgagt tcctcctcgg ttggtgcgtg accggtgtag aggtggatgt
     5941 ggtggtttgg gccgaaatga tttttcaggt atgtacagaa ctcaaccacc cggtcaagct
     6001 caagcagcgg ttctccaccg gtaacccccg ttcccagagc gtccataacc tcagcttcgg
     6061 ggattgcctc ctccggtttt gtgatctttt catcatttgc aaagattaca tccacgtttt
     6121 tccgctcctc tgagagcgga cagtaccagc agtcgcgatc acaccttccg gtaatgaaga
     6181 ggacaagctt tgctccttga tagcagagcc tgcatccctc actaagattt ttcgggagtt
     6241 tgtcttttgc ttttgccatt gttatgctgt catttcgttc agccgtgcaa gtgttgcttc
     6301 acgaccgatt gcttccagga accagccgag acgcggtccg cggtctgcac caaggaaggc
     6361 gcggtagatg gcggtgaaca gttcctttcc ggtgatacct gcggatgccg cagcatcgta
     6421 gactgcattg tgcaagacat cggccttcca ttcacccgca gcgatcgcag ttgcatatgc
     6481 agcaacggct gccttaacgt tttccggttc tgctgctgca atctccggaa gggtcggctg
     6541 gattgcgaac ttcacatctt ccggggcgta gcgttcaagc cacattcttg ccctgttcgc
     6601 ctgatccagt actgcattcc tgtccacaat ctcgtagccg ctgcgtttca ggatatcgaa
     6661 gataccctca tcgttctggg caatctggac taccgtgacc atgtgacgga atggaatcgt
     6721 ggtctgcggg gaggcgataa ccgagagttc gtactcgcgt gaatcaccag atgctgcggc
     6781 gcgatcgtac tcatcgatta accggagaag acccatgccg ggatcaaacg cgatggtctt
     6841 gtccggtttt gtctttgcga tcatgtaacg gagcacctct ggcgggacga tgtcaagcat
     6901 atcgcggatg gtcagaacaa cacctttgga ggagtgcatc tcaccgcggc ccttcaggct
     6961 gatccattca taggttactg gga
//
LOCUS       DALTZZ010000173         2365 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_27536,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000173 DALTZZ010000000
VERSION     DALTZZ010000173.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27536"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="O0X12_03250"
     CDS             <1..366
                     /locus_tag="O0X12_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HJK53208.1"
                     /translation="EEDLYADLIQEVNKLEMIVAIGSNATYLSDAATVLLPSAALNEY
                     PGTVTNWEGRVQKVRAASAVPEDAKCPCEIISLLSDGKYAWEDKAAIFADLAAAVPAY
                     TDIAYEEIEKPDGVFIKEE"
     gene            370..1626
                     /locus_tag="O0X12_03255"
     CDS             370..1626
                     /locus_tag="O0X12_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HJK53209.1"
                     /translation="MTAKGDMVYAWTTSSDIHGECGGAVTALLQHALASGMVDAVLAV
                     CRGADLYDAKLELITDPAKMPKSAGSLHCGTLLSAKMVKKYLGGAKDMKIAVPVKGCD
                     AMAFYELAKRQQINLDNVILIGLNCGGTVSPMIAREMIAEKYEVNPDAVFKEEIDKGQ
                     FIIEYTDENGEHQHKGISIEKLEEEPEGYGRRQNCQRCKCKVPRQADLACGNWGVIGE
                     KAGKATFVEVCSEKGAALLDAAVSAGAVATCAPEPKGLEIRGKVENAMLKLGDKNRAH
                     QFAELGEGRERLEKIMKLSSRCIACRACIENCPICYCVECSTAKPHLVRPGQVPPDFM
                     FHLIRFAHIADSCINCGQCQELCPMDIPNALFMHAQQVELEKMFGHKPGYDMTMPVLS
                     YAEEMEERARLNATGSDMIFENVFKE"
     gene            1726..2313
                     /locus_tag="O0X12_03260"
     CDS             1726..2313
                     /locus_tag="O0X12_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HJK53210.1"
                     /translation="MIDSFIEGNKIFLEQDFERKKDRYMTLVESQHPTVLWIGCSDSR
                     VNAERITHCRAGELFTHRNIGNIVPTHDWNFATVLEYAVRHLKVKDIVICGHSDCGAL
                     KALDADMTGDAYIPMWINNAREAQVRVDARRGKPATTPEEKAARKKEIEIENIRLQME
                     HLRTYPLVTEAEKNGKLEVHGLYYDLNTGVLSKIA"
BASE COUNT          641 a          528 c          638 g          558 t
ORIGIN      
        1 gaagaggacc tctatgcaga tctcatccaa gaagttaaca aacttgagat gattgtcgca
       61 attggcagca atgcaacgta cctttctgat gctgcaacgg tattactccc ctctgcagct
      121 ttaaacgaat acccaggaac agtcaccaac tgggaggggc gcgttcaaaa ggtacgggca
      181 gcatctgcgg tcccagagga tgccaaatgc ccatgtgaga tcatcagcct actctctgac
      241 ggaaagtatg catgggaaga caaagcagca attttcgcgg atcttgctgc agcagtccct
      301 gcatatactg atattgcgta tgaggagatc gaaaagcctg atggtgtatt cattaaggag
      361 gaatgagata tgacagcaaa aggtgatatg gtatatgcgt ggacaacgtc cagtgatatt
      421 cacggagaat gcggaggggc agtaacagct cttttgcagc acgcacttgc aagcgggatg
      481 gttgatgcgg tacttgcagt ctgccgcggg gcggatctgt atgatgcaaa actcgaactt
      541 atcacagatc ctgcgaagat gccgaagtct gcaggatctc tgcactgtgg aacacttctt
      601 tcagcaaaga tggtaaagaa gtatctcggc ggagccaaag atatgaaaat cgcagttcct
      661 gtaaaaggct gcgatgcaat ggcgttctat gagcttgcaa aacgccagca gatcaatctg
      721 gataatgtga ttttaattgg cctgaactgt ggaggtacgg tttctccgat gatcgcacgc
      781 gagatgattg cagagaagta cgaggtaaat cctgatgcag tcttcaaaga ggagatcgat
      841 aaaggacagt tcatcattga gtatacggat gaaaatggtg aacaccagca caaaggtatc
      901 tccattgaga agctggaaga ggaacctgag ggatacggcc ggagacagaa ctgtcagcgc
      961 tgcaaatgca aggttccacg gcaggcagat cttgcctgcg gaaactgggg cgtcatcggt
     1021 gaaaaggcag gaaaggcaac ttttgtcgag gtctgctcgg agaaaggtgc ggcactcctg
     1081 gatgcagctg tcagcgcagg ggctgttgca acctgtgcgc cagaaccaaa gggccttgaa
     1141 atcaggggta aagttgaaaa tgcaatgctg aagcttggtg acaaaaaccg tgcccaccag
     1201 tttgcagagc tgggcgaagg acgggagcgg ctggagaaga tcatgaagct ctcatcccgc
     1261 tgtattgcat gccgtgcttg tattgagaac tgtccgatct gttactgtgt cgagtgttca
     1321 acagcaaaac cgcatctggt gcggcccgga caagtgcccc cggacttcat gttccattta
     1381 atccggtttg cccacattgc tgactcatgt atcaactgcg gacagtgtca ggaactgtgt
     1441 ccaatggata ttccaaatgc actgtttatg cacgcccagc aggttgagct ggagaagatg
     1501 ttcggtcaca agccgggtta cgacatgacg atgccggtcc tctcctatgc tgaggagatg
     1561 gaagagcgtg cacgtctcaa tgcaaccggc tcggacatga tctttgaaaa cgttttcaaa
     1621 gagtaatctc tctcacaact ttttccggat gcgttgccgg tctgtcgtga gacagaccgt
     1681 gtgcgtctcc tctttttcac aatagacaac aaggtggtac tgaaaatgat tgatagtttc
     1741 atcgaaggca acaaaatatt tctcgaacag gattttgagc ggaagaagga tcggtatatg
     1801 actctggtcg agtcccagca cccgacggtt ctgtggatcg gttgttctga ttctcgtgtc
     1861 aatgccgaac gcattacaca ttgtcgtgca ggagagctgt ttacacacag aaacatcggc
     1921 aacattgttc cgacgcatga ctggaacttt gcaacggttc tggagtacgc ggtccgtcac
     1981 ctcaaagtca aagatattgt tatctgtggt cattccgact gcggtgcact caaagcactt
     2041 gatgcagata tgacaggtga tgcctatatt ccgatgtgga tcaacaatgc ccgggaagcc
     2101 caggtgcgtg ttgatgcacg gcgtggtaaa ccggccacca ctcccgagga aaaggccgca
     2161 cggaaaaagg agatcgagat cgagaatatc cgtctccaga tggaacatct cagaacgtac
     2221 cctctggtca ctgaagcaga gaagaacgga aagttagaag tccatggatt atactatgat
     2281 ctcaataccg gcgttctttc caagatcgcc tgagtgtttt tgatcatctt ctttttttat
     2341 cttgcatcgc cggtgaaaat attac
//
LOCUS       DALTZZ010000174         1862 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_27651,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000174 DALTZZ010000000
VERSION     DALTZZ010000174.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27651"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="O0X12_03265"
     CDS             <1..634
                     /locus_tag="O0X12_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53211.1"
                     /translation="DLLYVLQIDGWGRSVVRELADARIKAVILPRHVYTRAQEQHLIE
                     EFREAGLPVLSGADLSPRVKGKIGVADTAAVEEALAAWDASQTVYSKEKKAAVINSMV
                     KEYQVERVREVRELGIDPATVIPERYLRTAPEPKKKETIKTVVRKPVENKTKEVAGQQ
                     KPVPPKPLPPEEKEELPDETQTPDQNASDLFTAVLTEYREKRKKEISGDE"
     gene            634..1494
                     /gene="thiL"
                     /locus_tag="O0X12_03270"
     CDS             634..1494
                     /gene="thiL"
                     /locus_tag="O0X12_03270"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HJK53212.1"
                     /translation="MDDRQLLESIRDLIGRTETSDDCAAFPLDNGKKILVSTTDMLHE
                     TTDFPKGMTEWQMGWMSVAVSLSDVASCGAQPTQVLVAAGLDRAERLKPIMEGAVACA
                     KACDAKIAGGDIDSHTELTIVTTAFGVVDEEYYCTRSGAQLGDLVCITGRPGAAQAGL
                     DGYSGYWENLVMPKPQVFTGQRIAHAGATAMMDVSDGLAISLYDMSEASGVGFALREN
                     HLPLLPIADAKRYFLYGGGDYGLLFTIPQEKFVKMDVPVSVIGEVIEGDGVRMGNELV
                     EKRGYAHHIA"
     gene            complement(1600..>1862)
                     /locus_tag="O0X12_03275"
     CDS             complement(1600..>1862)
                     /locus_tag="O0X12_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53213.1"
                     /translation="VVSSLAVGDDEEIVVTTAAGVVMRTQAGDISIQKRSTQGVRIIR
                     VDDGDKVTSVAVVPAEEEEDVPSDPSGPADDAQKSLFTDIEE"
BASE COUNT          525 a          440 c          507 g          390 t
ORIGIN      
        1 ggatttactg tatgttctcc agattgacgg atggggaagg agcgttgtgc gggaactcgc
       61 cgatgcgcgg ataaaagctg ttatcctccc ccgccacgtc tatacccgtg ctcaggagca
      121 gcatctgatc gaggagttcc gggaggcagg actgccggtt ctttccggag cagatctttc
      181 cccccgagta aagggaaaga ttggtgtggc agacacagca gcggttgaag aggcacttgc
      241 cgcatgggat gcgtcacaaa cggtgtactc caaagaaaag aaagctgccg tcatcaactc
      301 catggtaaaa gagtatcagg tggagagagt acgggaggtg cgggagttag gaattgatcc
      361 tgccacggtc atccccgagc ggtatctgag aaccgctcct gaaccaaaaa agaaggaaac
      421 tatcaaaacc gttgtcagga aacctgttga gaacaaaaca aaggaagtcg ccggacaaca
      481 aaaaccagta cctccaaagc cactgcctcc cgaagaaaaa gaagaacttc ccgacgaaac
      541 ccaaacacct gatcagaatg catctgatct gttcactgcg gttcttacag agtaccgaga
      601 aaagagaaag aaagagatct ccggtgatga gtaatggatg atcgacaact gcttgaatca
      661 atccgcgacc tgatcggccg caccgaaact agtgacgact gtgcagcatt tccgctggac
      721 aacgggaaaa agatactcgt atcaacgacc gatatgctgc atgaaactac cgattttccc
      781 aaaggaatga cggagtggca gatggggtgg atgagtgttg cagtgagtct ttcggacgtg
      841 gcaagctgcg gcgcacaacc gacccaggta ctggttgccg caggtcttga tcgtgcagaa
      901 cggctcaaac cgatcatgga gggagctgtt gcctgtgcaa aggcctgtga tgcaaaaatt
      961 gccggaggag atatcgacag ccacacggaa ctgacgattg taacaaccgc ttttggagtg
     1021 gtagacgagg aatattactg cacccgttcc ggggcacaac taggagatct tgtttgtata
     1081 accggaagac ccggggcagc acaggcaggg cttgacgggt acagcggata ctgggaaaat
     1141 cttgtgatgc caaaaccaca ggtgtttacc ggacagcgga ttgcacatgc cggggctact
     1201 gcgatgatgg atgtctccga cggccttgca atctcactgt atgacatgag tgaggcatca
     1261 ggagtcggat ttgctctgcg ggaaaatcat ctgccgcttc ttccgattgc cgatgcgaag
     1321 agatatttcc tctatggagg aggagattac gggcttctgt tcaccatccc gcaggagaaa
     1381 tttgtgaaaa tggatgttcc ggtctctgtc atcggagaag taattgaggg agatggagtc
     1441 cgtatgggaa atgagctggt ggaaaagcgc ggatacgcac atcacatagc ctgaaagaat
     1501 tattcatatg ttgcgataat gaaaaattca tttttaaaaa tgggaatatt tccaaaataa
     1561 aaagcgagaa ctgcaaagat ttcagagaaa ttgaggagat tactcctcaa tatccgtaaa
     1621 caggctcttc tgggcatcat ccgcaggtcc agatgggtca gagggtacat cctcctcttc
     1681 ttctgccgga acgaccgcaa cactggtgac tttgtcgccg tcatcaacac ggatgatccg
     1741 aactccctgc gtactccgct tctggatcga aatgtcgccg gcctgcgtac gcatcacaac
     1801 accagctgcc gtggtaacca caatctcctc atcatcaccg acggcaagtg aagagacaac
     1861 ac
//
LOCUS       DALTZZ010000175         4712 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_27660,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000175 DALTZZ010000000
VERSION     DALTZZ010000175.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4712)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4712)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4712
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27660"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            1..1160
                     /locus_tag="O0X12_03280"
                     /pseudo
     CDS             1..1160
                     /locus_tag="O0X12_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
     gene            complement(1968..2231)
                     /locus_tag="O0X12_03285"
     CDS             complement(1968..2231)
                     /locus_tag="O0X12_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53214.1"
                     /translation="MDSYTIRKIDARRFTVTVTSPSKQSWRTLESAGLSVTDEKRASY
                     KGGECYSWTVQARQPGIYELKMIQQSDDGAYRKVVIPIIAEAS"
     gene            complement(2462..2545)
                     /locus_tag="O0X12_03290"
     tRNA            complement(2462..2545)
                     /locus_tag="O0X12_03290"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2509..2511),aa:Ser,seq:cga)
     gene            complement(2677..4263)
                     /gene="thsA"
                     /locus_tag="O0X12_03295"
     CDS             complement(2677..4263)
                     /gene="thsA"
                     /locus_tag="O0X12_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HJK53215.1"
                     /translation="MSSRSGQPVVILRDNVERVPGQEALRSNIMAAKVLGNTVRTTLG
                     PRGMDKMLVTQGSDDIVITNDGATILHQIHVQHPGAKLVVEVAETQDDECGDGTTTAV
                     VMVGSFMEQAENLIDAGVHPSVIAKGYNLGMMKALEILDTLAIEVTPKDTAMLKQIAK
                     TAMTGKSIEMIMDKACDVVVEAVSNVAVTSGKKTTVNEDDILVKTKRSETMDAEMIKG
                     VLIDKTRLDALMPKKIKGVKAAFIANPLEITKTQTKSKIKITSHEQLEAFSVAERETL
                     RAMAEKFVENGVNVVLCQKGIADPVQYYLAENGIYALEFVPEKDLKYAAKALGGQIVN
                     KPEDLSPDVIGTAGNLEMLEDIEMTKLSGCKNPQAVTILLRGSSQHLVDELERAIEDA
                     TRVVQDVVEDGAYLIGGGSVETELALRLREYAATEGGRVQLAIEGYAKAFEIIPKTLA
                     ENSGFDTVDKVIDLRQAHATGEKYAGLNVFTGKVVDMKSEGVVEPKRVKRQAIQSASE
                     TAMLLIRVDDMMISKGADQM"
     gene            complement(4343..>4712)
                     /locus_tag="O0X12_03300"
     CDS             complement(4343..>4712)
                     /locus_tag="O0X12_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase-like protein"
                     /protein_id="HJK53216.1"
                     /translation="FEAATGGEEEVDAIVGISISGVPLATLIAAEEGLNLSVYHPSKH
                     NTDSGTGCISGNFSKISGKRCILVDDVITSGNTLTELVGYLRRHNATPVAILVIFDKR
                     GMTEVDGVPVYSLLSIRLID"
BASE COUNT         1077 a         1190 c         1227 g         1218 t
ORIGIN      
        1 cctcggtaat gacgggtatc tcccgcatta atcttccgga gacaaggcct gtcggtaacc
       61 gcggggcggg tggcggcatt gtggtcggaa cgtatgaggg tgtggacaat cttctccgca
      121 aagggtccgg tcttcacaac atcggtacaa tcgttatcga tgaggtacag atgctcgagg
      181 atccggaacg cggacatcgt cttgacggcc ttattgcccg actgaagtat gctgccccca
      241 aagctcagtt tctgtatctt tcggcaacga ttggatcccc tcatctgctt gcgaaaaagc
      301 ttggagccac cctggtctcg tatgctgatc ggccggtcgc tcttgagcgg catctcatct
      361 tcacagaaaa ggatgcgaag gttccctaca ttaagaaact cgtgtttgag gagtacaata
      421 aaacttcatc aaaaggttac cgcggtcaga gtatcatctt cacgaatgcc cgtacacgct
      481 gccatacgat agctgatgcc attggaaagc ttgctgcccc gtaccatgcc ggtcttactt
      541 cgcaggaacg gcgtgcagtt gaaaaacagt tcgaggatgg taaacttgcc tgtgttgtaa
      601 caactgcggc gcttgcagcg ggtgtggact tccctgcatc acaggtgatc tttgacgcac
      661 ttgcaatggg gatcaactgg ctgacagtac aggagttcca tcagatgatg ggccgtgcag
      721 gtcgtcctga ttttcatgat attggccgcg tggttgttct tgcagagccg ggagcatcgt
      781 attcacgcga gacgaaactg actgaggaag aggtcgccat ccgccttctc aaaggcgaca
      841 tggaagaggt tgctcctgtc tacgatattg aggaggcctc ggaagagttt gcggcgaacg
      901 cgatcgtgtg ccgcggacgt gaagcagata ttgtgaaggt cggtgagagc atggtgggtt
      961 gtggtgagga tccactgccg gaacttttgc ggcacaagct tgtcaaaaag aatggaggtg
     1021 ttctggaact gtctccgctt gggcgtgtga tggccgaaca gttcatcggc atggagaagc
     1081 tccttgagat cgatcgtctt gtccgggtta tggatgatcc gctggaactg atcgcagaga
     1141 ttgagtgtgc cgaagaggaa cgtgataaag aggcacgacg tgccgatcgc aggatgaccg
     1201 agaaaaagga gtcagactgg atggctggta agaaaacttc gtctgccctg tcgtctgaga
     1261 agaagatacg cattgaggct ccacctgcct gggttgccga acggaagtct gcgatcgttg
     1321 cggcgaaacg ggaaaacgag agcgatcgta aacttcgctc cggcaagacc gaaccggagc
     1381 gaaaactccg gcgtgaacat gacaggggaa ccaccgaggt aaaagcacca cgtcgtgcaa
     1441 gtaaggaact agaggatcgg atcgaacgta agaagcgtga ggcggccgca tttatccctg
     1501 ctccgaaacg aaagaagact gatgagtggg atgcaattga ggctggtatt gatcagcgcc
     1561 gtgctggtaa atatgatgat gcactgaaga tcctcggcag ttacgttgcg gatcatccct
     1621 ccgacagtcg tgcactgacc gagcttggca aggtgtatga tctccgcggc gatcgcgaca
     1681 cggcttactc ctgctatcag cgtgcggcag cggctgacac tcggaaccgc gaggcgattg
     1741 atcggatgaa tgcctatctt attggaatta cggtggacat tgatgtggac gctcctgcca
     1801 agcgtcagcc gacaggtgtc gtgaagaaga aaaaacagtg aaacctcact tgtacttttt
     1861 atttttccct ctgcattctt ttgctcatgg gatctcctgg aaaaaatctt tgtcttccat
     1921 gaaatgcaca attcaaaaaa agatcaggtg aaagtcctaa taccacatca ggaagcttcc
     1981 gcaatgattg ggataaccac tttgcggtat gccccgtcat cactctgctg aatcattttt
     2041 agttcataaa tgcctggctg tcttgcctga acagtccagg aataacattc gccacccttg
     2101 tacgacgcgc gtttctcatc cgtcactgaa agtcccgcgg actccaatgt ccgccaagac
     2161 tgtttgcttg ggctcgttac ggtcaccgta aaccttcgcg cgtcgatttt tcggatcgta
     2221 tatgagtcca tgttctcttt ctccgttctg gattggtttt tttctgataa ttctgctgat
     2281 tagcaattga tgatatattg ttatcctata taaatgatgt cgtcatattt tgcgcataat
     2341 tttgcaaagg tcctgtatct tttcacagat attctgtcga atttttgttc gacaggtttt
     2401 cgtgataaca ctgggtaata acaggatccc gatgtactcg tagtattttg taaaataaca
     2461 acgccgaggg ggagatttga actcccgagg tgttaccacc agaggctttc gaggccaccg
     2521 cccttccgga ctagactacc tcggcacatc cgtttactgc tcaaaacagc atgcgcgaca
     2581 agatctccgc tgatgaaagc aaaacatgat ctctcgcagc accttactgc ctatacacat
     2641 cgacttgtgg tctaaaaaag atagtgtttt ttggtattac atctggtcgg cgcctttgct
     2701 gatcatcatg tcatctacac ggatcagcag cattgccgtc tcggatgcgc tctggatcgc
     2761 ctggcgtttt acacgcttcg gctccacaac gccttctgac ttcatgtcaa caacctttcc
     2821 ggtaaacacg ttcagacctg catacttctc gccggttgca tgtgcctggc ggagatcaat
     2881 gaccttgtca acagtatcaa aacctgagtt ttctgcaagt gtcttgggga tgatctcaaa
     2941 tgcctttgcg tatccctcga ttgcaagctg cacacggccg ccctcggttg ctgcgtactc
     3001 acggagacgc agggcgagtt ctgtctcaac ggatccgccg ccgatcaggt atgctccgtc
     3061 ttcaacgaca tcctgaacca cacgggttgc atcctcaatt gcacgctcca gttcgtccac
     3121 gaggtgctgg gaagatccgc gcagaagaat ggttaccgcc tgcgggttct tgcatccgga
     3181 gagcttggtc atctcaatgt cctcaagcat ctcaaggttt cctgcggttc cgatcacatc
     3241 ggggctgaga tcctctggct tgttgacgat ctgtccgccg agtgcctttg cagcatactt
     3301 cagatccttc tccggcacaa actcaagtgc atagatacca ttctcggcaa ggtagtactg
     3361 caccggatct gcaattccct tctggcagag gactacattg acaccgtttt ccacaaactt
     3421 ctcggccatt gcgcggagtg tctcacgctc tgcaacggag aatgcctcaa gctgctcgtg
     3481 ggaagtgatc ttgatctttg atttggtctg ggtcttggtg atctcaagcg ggtttgcaat
     3541 gaatgcagcc tttacgccct taatcttctt cggcatcagt gcgtcaagtc tggtcttgtc
     3601 aattaagaca cctttgatca tctcagcgtc catcgtctcg ctgcgctttg tcttgacaag
     3661 aatgtcgtcc tcgttgaccg tcgtcttctt gccggaggta accgcaacat tgctgaccgc
     3721 ctccacaaca acatcacagg ccttgtccat aatcatctca atggacttac cggtcattgc
     3781 tgtctttgca atctgcttga gcattgcggt gtctttcgga gtaacttcga ttgcgagggt
     3841 atcgaggatc tcaagtgcct tcatcatacc aaggttgtac ccttttgcga taactgacgg
     3901 gtgaacacct gcgtcgataa ggttctcggc ctgctccatg aaggatccta ccatgacaac
     3961 cgcggttgtg gttccgtcac cgcactcgtc atcctgtgtc tctgcgacct caacaacgag
     4021 ttttgcaccg gggtgctgta cgtgaatctg gtgaaggatg gttgcgccgt cattggtgat
     4081 gacgatgtca tcgcttccct gcgtaaccag catcttatcc ataccgcggg ggccaagagt
     4141 ggtccgaaca gtgtttccaa ggacctttgc ggccatgata ttggagcgga gcgcctcctg
     4201 tccaggcacg cgctcaacgt tgtcccgtaa aataacgaca ggttgtcctg atctgcttga
     4261 catgaaatct attatctcct gtactcgtgt gataaaaatg gaattgaact tctatataaa
     4321 atatgtgtat tcggatcttc cctcagtcga taagccgaat agagagcagc gagtacaccg
     4381 gtactccgtc gacttcggtc atgccgcgct tatcgaagat gaccagaatt gcgacaggcg
     4441 ttgcattgtg tctgcggagg tatccgacca gttcggtcaa ggtgttgcct gacgtaatga
     4501 cgtcatcaac gagaatacag cgttttccgc ttatcttgct gaagtttccg gagatgcatc
     4561 cggtaccgga gtctgtgttg tgtttggagg gatggtagac ggagagattg agtccctctt
     4621 ctgcggcaat cagcgttgca agcggtacac cggagatgga gataccaacg attgcatcca
     4681 cttcctcctc accaccggta gctgcctcaa ac
//
LOCUS       DALTZZ010000176         7036 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_27881,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000176 DALTZZ010000000
VERSION     DALTZZ010000176.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27881"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="O0X12_03305"
     CDS             <1..559
                     /locus_tag="O0X12_03305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK53217.1"
                     /translation="MHTALGLDSAVSGLLLMISSVITALIGIPVGAWCDRIGCRTPCI
                     LAAICRIAFCTVLLLIVPSYGILALLPALICMGLAFGISGGPATTRIVQHAPEGEAGT
                     GTSVMITSDFLGGVIGVAAYAVVFSLAVPASVGVAVSDVPASLFVEGFHATAVLGLLC
                     GIVTLILSAVVPNLVTKREDLVVSE"
     gene            625..1668
                     /locus_tag="O0X12_03310"
     CDS             625..1668
                     /locus_tag="O0X12_03310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJK53218.1"
                     /translation="MTAADISRIRVAGFRSIADAAFPLDRINILIGSNGSGKSNILGI
                     FELLHAVTTGNLQTYVTECGGAEILFRYGSGQTKTIMLRVEFGDSYYELHLLPSGNGC
                     RIDREHLTLITDDATLQLVSSDTAETGIFAGVQADASWESFRLRLQSWTTYRFQSRCT
                     GAEDERRLTEFLHRLQETAPEHYEKIVDTIRLVFPQFGTFQFREVNGVVKLFWTDALV
                     KDHMFGLGALSGGTARFINLAVLLLQPSPPHLILIDEPELGLHPYAILVLADLMKLAS
                     RTSQLIVSTQSVQLLNAFGPHEDNAGSRILIVEQDHGATAVTVPTPESLADWIEEYTI
                     GDLWQMNILGGNP"
     gene            1665..2363
                     /locus_tag="O0X12_03315"
     CDS             1665..2363
                     /locus_tag="O0X12_03315"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HJK53219.1"
                     /translation="MTLLLKIHVEGFTEASFASRILRPHFEEYDCSIAVIINKTSNSH
                     GIAHRGGLSHYEQFRVNTRRLLKNKNAVVTTMIDYYGLPTDFPGMTDCGKFSTAPDRV
                     SYLERMLDEDISGDWLSEENFIPYIQLHEFEALLFSDISVVDRVLSVGRESKYAELEE
                     IRRMYAPEEINTSPETAPSKRLKKLYPHYSKTVEGMAIAERIGLAKMRKCCPHFNAWI
                     TELEQVATSRREKE"
     gene            complement(2474..2947)
                     /locus_tag="O0X12_03320"
     CDS             complement(2474..2947)
                     /locus_tag="O0X12_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HJK53220.1"
                     /translation="MSEITISEDAMRMIAQFENLTGAGARDCVVDDKFGRILFVINPG
                     EMGLAIGKKGASIKKASDAFGKKVEVVEYNPDKVQFIRNCFLPVQVMTVTFEEDEDGE
                     IAYVEVRDDDRGLAIGKEGRNIIKAKKLAFRQFDVSNVELKQDEVEEEDEFDDEL"
     gene            complement(2949..3239)
                     /locus_tag="O0X12_03325"
     CDS             complement(2949..3239)
                     /locus_tag="O0X12_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30e"
                     /protein_id="HJK53221.1"
                     /translation="MDFNLSLRRAIKTGKVVLGQNSVEKVIAENKAELVIVAKNAPAK
                     VRAIINAADNLPLYEFNGSSRQLGKECGRDHMISVLAIVDAGESDILSLKRA"
     gene            complement(3464..4321)
                     /locus_tag="O0X12_03330"
     CDS             complement(3464..4321)
                     /locus_tag="O0X12_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HJK53222.1"
                     /translation="MRLAPECRACLLSRVVAEAKHAGADADTVSAALERCTELYDSRA
                     DEPIGASVIAGAVHRCCYAVIGSKDPYADLKVQDNAIAEKVVQDVQPMLSNVHDYIVA
                     AVIGNAMDYGVAGHEIAKDFTGYFRKMFEQGLAQDDTEKILALAKRVVYCTDNCGEAV
                     FDKLLCAELRKHGSHVTIVVKDQPMLNDVTMQEAKSLHLDEAADAVYHAGGGAQLGLH
                     PDLYPPEVVAAMQNATLIIAKGLANYESLTEYTGLPPIAYLMTVKCEPVARHVGAKKG
                     DLIAVLRHQ"
     gene            complement(4350..4422)
                     /locus_tag="O0X12_03335"
     tRNA            complement(4350..4422)
                     /locus_tag="O0X12_03335"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4386..4388),aa:Pro,seq:ggg)
     gene            complement(4509..4973)
                     /locus_tag="O0X12_03340"
     CDS             complement(4509..4973)
                     /locus_tag="O0X12_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HJK53223.1"
                     /translation="MMQDPEPFTRTRFALAQGCGAVTIAIYVLIAILLVVAALMGVAE
                     TVTLVMTALQDPTQHALSNVLQAILLIIVIATLVDMVASYVRAGRVLVRPILIAGITT
                     MVRRLLVSDLTFIDIIGTTIVILGLTVAMVYVGRDEMEMMEHLKDMKRSEDQ"
     gene            5267..5340
                     /locus_tag="O0X12_03345"
     tRNA            5267..5340
                     /locus_tag="O0X12_03345"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5301..5303,aa:Lys,seq:ttt)
     gene            5539..5787
                     /locus_tag="O0X12_03350"
     CDS             5539..5787
                     /locus_tag="O0X12_03350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HJK53224.1"
                     /translation="MTRLNVLLHEMVPDHQIMTPEEVAKLLADYDITEEHLPKMYHDD
                     PAVKACGAKPGMVIRITRKSQTAGEATSYRLVVRRPKK"
     gene            5803..>7036
                     /locus_tag="O0X12_03355"
     CDS             5803..>7036
                     /locus_tag="O0X12_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HJK53225.1"
                     /translation="MIDRSVLSGAYFSQEHVARHQLDSFNNFVEYNLQKVVNEQRIVE
                     TEIVNRGKNQEPVWVELGEIRVEKPIVREADGSQSKLYPTEARLRNLTYAAPMVLTMT
                     LHQGEKEFEPQETTIGQLPVMIGSKVCNLCGLSDAERTEQGEDPSDPGGYFIVNGTER
                     VLMTLEDLASNKIMTEYTERYNEQIHVSKVFSQFRGYRALVVVEKNKKNLLDVSFPSV
                     AGHLRFADLMRALGLETDEEIVSAVSLSEDILTYMMQNLEESECATSEEGIMYVGKKL
                     APNQTKDYQRKRAEFVLDSYLLPHLNYLIPSHLKPGDESYELYAKDVRVAKAEFLGRM
                     AEACFDLALNKRRIDDKDHYSNKRLKLAGDLMEDLFRISLNRLTRDVKYQLERASMRH
                     RELAIGTVVRADVLTERLI"
BASE COUNT         1734 a         1775 c         1674 g         1853 t
ORIGIN      
        1 catgcacaca gccctcggcc ttgactcggc agtgtctggt ctgctgctga tgatctcgtc
       61 cgtcattact gccctgatcg gtattcctgt tggcgcctgg tgtgatcgta ttggatgccg
      121 tactccgtgc attcttgcgg caatctgtcg aatagcattt tgtactgttc ttttgctgat
      181 tgttccgtcg tacggtattt tggcactgct tcctgccctt atctgtatgg gtcttgcatt
      241 cggtatctcc ggcggtcctg caacaacccg tattgtgcaa catgcaccgg agggtgaagc
      301 aggtaccggc acctcagtga tgataacttc ggattttctt gggggagtta tcggggttgc
      361 cgcatatgca gtggtgtttt cccttgcagt tccggcgtct gtcggcgttg cggtgagtga
      421 cgttcccgca tctctgtttg tggagggttt ccacgcaacc gcagttcttg gtctgctctg
      481 cggcattgta acactgattc tgtcagcagt tgtcccaaac ctggttacca agagagagga
      541 tctggtagtc agcgaataaa gatcttcttt gaatgacgta agatgctgta tctttaatac
      601 cgtttgcagc gatactgaca aataatgact gctgcagaca tctcaaggat acgggttgcg
      661 ggatttcgct ctattgcgga tgctgcgttt cctcttgacc gcataaatat cctgatcgga
      721 tcaaacgggt cggggaaaag caatattctt ggtatttttg agcttcttca cgcggtcact
      781 accggaaacc tgcagacata tgtcacagaa tgtggcggag cagagatact gttccgctat
      841 ggttctgggc agacaaaaac tatcatgctg cgtgtggagt ttggagatag ttattatgaa
      901 ctccatcttc ttccctctgg aaatggatgc cgcattgatc gtgaacatct tacccttatt
      961 accgatgatg caacgctgca gctggtgtca tccgatactg ctgagaccgg aatatttgca
     1021 ggtgttcagg ctgatgcatc atgggaaagt ttccggcttc gcctgcagag ctggacaacc
     1081 tatcggttcc agagcagatg tactggagca gaggatgaga gacgactgac tgaattttta
     1141 catcgattac aggagactgc cccagagcat tatgaaaaga ttgttgacac cattcggctt
     1201 gtctttccgc agtttggaac ttttcagttc cgtgaagtaa acggtgtggt aaagctgttc
     1261 tggacggatg ccctggttaa ggatcacatg ttcggccttg gtgctctctc tgggggaact
     1321 gcccgtttca tcaatctcgc agtccttctc cttcaaccct ctcccccgca tctgattctg
     1381 attgatgaac ccgaacttgg tcttcatcca tatgcaatac tggtgcttgc agatctgatg
     1441 aaacttgcaa gcagaacctc acaactgata gtctccacac agtcagtaca gcttctgaac
     1501 gcgtttggac cgcatgagga caatgctggt tccagaattc tgattgtaga acaggatcac
     1561 ggtgcaacgg cggttactgt tccaacccct gagagtcttg ctgactggat cgaagaatac
     1621 actatcggtg acctgtggca gatgaatatt ctgggaggaa atccgtgacc cttctgttga
     1681 agattcatgt ggaaggattc accgaggcat catttgcatc acgaattctg cggccgcact
     1741 ttgaggagta cgactgcagt attgcagtta ttatcaacaa gacaagcaac agccatggta
     1801 ttgcccatcg cggaggattg agtcattacg aacagttccg ggtaaacacc cgtcgcctgc
     1861 tgaaaaacaa aaatgccgtg gtgacaacaa tgatcgacta ttacgggctc ccgactgatt
     1921 ttcccggcat gacagactgt gggaaatttt ctacggcacc cgatcgagtt tcgtatcttg
     1981 agcggatgct tgatgaggat atcagcggcg actggctttc cgaggagaac tttattccat
     2041 atattcaact gcatgagttt gaagcactgc ttttttcgga tatttcagtt gtggatcgtg
     2101 ttttgtctgt aggtcgcgag tctaagtatg cagaacttga agagatccgg agaatgtacg
     2161 cacccgaaga gatcaacaca agcccggaga ctgctccttc aaaacggttg aaaaaactgt
     2221 atccgcacta ttcaaagacc gtggaaggaa tggccattgc cgaacggatc ggtcttgcaa
     2281 agatgcggaa atgttgtcca cactttaatg cgtggattac agagctggaa caggtcgcaa
     2341 catcacggcg cgaaaaggag taacctgttt ttttgtcgtg aaaactatct gttcacaaaa
     2401 atcaaactat tctgtgtgta ttacccagca gttcatttgc gggaatgtgt aaaaagagaa
     2461 aaacaagctg tgtttacagc tcgtcgtcaa actcgtcttc ttcctctact tcgtcctgtt
     2521 tgagctcaac gttgctgaca tcaaactgac ggaatgcgag tttctttgct ttgataatat
     2581 ttcttccttc tttgccgatt gcaaggccgc gatcatcatc acgaacctca acatatgcga
     2641 tctcaccgtc ctcgtcctcc tcaaacgtga cggtcataac ctgcaccggc aggaaacagt
     2701 tacgaataaa ctgaactttg tcagggttgt actccacaac ctcgaccttc ttgccaaacg
     2761 catcggatgc cttcttaatg cttgcaccct tctttccgat tgcaagcccc atctctcccg
     2821 ggttgataac gaagagaatt cggccgaatt tgtcgtcaac cacacagtcg cgtgcacctg
     2881 cgccagtcag attctcgaac tgcgcgatca tacgcattgc atcttcggaa attgttattt
     2941 cactcatatt atgctctctt caggctcaga atgtcggatt cgcctgcatc aacgatagca
     3001 agaacactga tcatgtgatc gcgaccgcac tctttaccga gctgacggga actgccgttg
     3061 aactcataga gcgggagatt gtctgctgcg ttaatgattg cccggacctt tgccggtgca
     3121 ttctttgcaa cgatgacaag ttctgctttg ttttctgcaa tgactttctc aacgctgttc
     3181 tgtcctaaaa cgaccttgcc ggtcttgatt gcccttctca gggataagtt gaaatccatt
     3241 tgtagattca ccagtttttg tggttagttc tgcactccga tccggcacca ggggaggtgg
     3301 cccccataaa atccgaatct tcccgcaggt cccgcagtaa agatacaact gcggcagtta
     3361 tccggtcagt caactgaact tcccgaggaa actctataca tattgtatct cgcagataat
     3421 gaatgtattc tacatctgaa aaataacgcg tgtagtctgg cttttactgg tgtctcaaca
     3481 ccgcaatcag atcgcctttc tttgctccca catgccgcgc aacaggttcg cattttaccg
     3541 tcatcaggta tgcaatcggc ggtagtcctg tgtactccgt taagctttca tagtttgcca
     3601 gaccctttgc aataatcagg gttgcattct gcattgcagc aacaacctca gggggataca
     3661 ggtccgggtg caggccaagt tgggctcctc ctcctgcgtg gtacaccgcg tctgctgcct
     3721 cgtccaggtg caggcttttt gcttcctgca ttgttacatc attgagcatc ggctgatctt
     3781 ttaccacaat cgtcacatgg ctgccgtgtt tccgaagttc cgcacatagc aatttatcaa
     3841 ataccgcctc cccacagttg tcggtacagt acaccacgcg tttggcgagc gcaagaattt
     3901 tttccgtgtc atcctgtgca agaccctgct cgaacatttt gcggaaatag ccggtgaagt
     3961 cttttgcaat ttcatgacct gccacaccgt aatccattgc atttccaata actgctgcaa
     4021 caatatagtc atgcacattc gaaagcattg gctgtacatc ttgtacaact ttctctgcaa
     4081 ttgcgttgtc ctgtaccttg agatcagcat acggatcctt cgatccaatc actgcatagc
     4141 agcaacggtg tactgcgcct gcaattactg atgcgccaat cggttcatcc gcgcgggagt
     4201 catacagctc cgtacagcgt tcaagcgcgg cagataccgt atctgcatcc gctcctgcat
     4261 gctttgcttc tgcaacaacc cttgagagca gacatgctct gcattccggt gcaagtctca
     4321 taacattatc acccaaacac aaaaacgaat ggggccgccg cgatttgaac gcgagtcaga
     4381 agacccccag tctcctagga ttccaggcta ccctacggcc cctcatacaa catcctatgg
     4441 atgaagtatc atacatatag gatgtacttc ccttattagt ttttactttg acatcggcaa
     4501 aaaaaggatt actgatcctc tgaacgcttc atatctttca gatgctccat catctccatc
     4561 tcatcgcgac caacgtatac cattgcaacg gtcagtccga gaattacaat cgttgttccg
     4621 atgatatcga taaacgtcag atcgcttacc agcagtcgcc ggaccattgt tgtgattcct
     4681 gcaatgagaa tcggccggac cagaacccgt ccggcgcgga cataggaggc aaccatatcc
     4741 accagcgttg caattacgat gatcaaaaga attgcctgca gcacatttga caacgcgtgc
     4801 tgtgtggggt cctgcagtgc tgtcatcacc agtgttacag tctcagccac gcccatcagt
     4861 gctgctacta ccagcagtat tgcgatcaac acatagatcg caatggttac tgcaccacaa
     4921 ccctgcgcaa gggcaaagcg agtgcgggta aacggttcgg gatcctgcat catgtctttc
     4981 cttccggtac gcagatgttt ctgcgtgatg tactcttact atggcggtcg cagtaatata
     5041 agaatgaggt tttgcgccaa attccctgtg ttcctttcgc cgcagacccg acactttatt
     5101 atttgcgaac gaacaaccta cttaacgcga aggcataatt gggggctgtg cagaagagat
     5161 tgtattctct tctcgaagca tgccgtgtag tcatctggaa tgactaaccg gtatgtatat
     5221 atatttttca atcccaataa tgatgctacc ttccagagga caacaagggc cagtagatca
     5281 gtcaggcaga tcgtcggact tttaatccgc aggtcggggg ttcaactccc tcctggcccg
     5341 cttacacgtc gccatgggtt tttaggatgt cgttggaatc aacaggacaa tcctatttac
     5401 ttttcggcag cgtgtattgg cgcgtccgtt ggacatctgt acaagcgtca ataaaaaact
     5461 ctgagacttt tatgtggtga gaactctgaa cacaagcaca gacatccgta tctatcaaga
     5521 tcttaaatta ggagctctat gacacgacta aacgtattac tccatgagat ggttcccgat
     5581 caccagatca tgactccgga agaagttgca aaactccttg ccgactatga cattaccgaa
     5641 gagcaccttc ccaaaatgta tcatgacgat cctgccgtca aagcctgcgg agcaaaaccg
     5701 ggaatggtca tccgcattac acgcaaaagc cagactgcgg gtgaagcaac atcttaccgc
     5761 cttgtggtca gacggccgaa aaaataacca gagtggatat acttgattga tagaagtgta
     5821 ctttccggag cgtacttctc ccaggagcac gttgcccgtc accagctgga ttcgttcaac
     5881 aactttgtgg aatacaacct tcagaaagtt gtcaacgaac agcgtattgt tgagacggag
     5941 atcgtaaacc ggggaaagaa ccaggaaccc gtatgggttg aactcggaga aatccgagta
     6001 gaaaagccga ttgtccgtga agcggacgga tcacagagta agttataccc aaccgaggca
     6061 cgtctgcgca accttaccta tgcagccccg atggttctta ccatgacgct ccaccagggc
     6121 gagaaagagt ttgaaccgca ggagaccacg attggtcaac ttccggtcat gattgggtcc
     6181 aaggtctgca acctctgtgg gcttagtgat gcggagagaa cagaacaggg tgaggatccc
     6241 agtgatcccg gaggatattt cattgtcaac ggaaccgagc gtgtgttaat gacgcttgag
     6301 gatcttgcct ccaacaagat catgaccgag tacaccgagc ggtacaatga gcagattcat
     6361 gtatcaaagg tattctcgca gttccgcgga taccgtgcgc tcgttgttgt cgagaaaaac
     6421 aagaagaatc tcctcgatgt cagcttcccg tccgttgccg gtcacctccg tttcgcagac
     6481 ctgatgcgtg ccttaggtct tgagactgat gaggagattg tgtccgcggt ctccctctct
     6541 gaagacattc tgacctacat gatgcagaac ctggaagagt ctgagtgtgc aacctccgag
     6601 gaaggtatca tgtatgtcgg taagaaactc gcaccgaacc agaccaagga ttatcagagg
     6661 aagcgtgcag agtttgttct tgacagctac ctcctgccgc acttaaacta cctcatcccg
     6721 tcccacttaa agccgggaga cgagagctat gagctgtatg ccaaagatgt ccgcgtcgca
     6781 aaggcagagt tcctcggcag aatggcggaa gcatgtttcg atctcgcctt aaacaagagg
     6841 agaatcgatg acaaagatca ctactccaac aagcgtctga agcttgcggg agacctgatg
     6901 gaagatctct tccgtatctc cttaaacaga ctcacccgcg atgtgaagta ccagcttgag
     6961 cgtgcaagca tgcgccacag agagcttgcc atcggtaccg tggttcgtgc tgatgtgttg
     7021 accgagcggt taattc
//
LOCUS       DALTZZ010000177         2321 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_27908,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000177 DALTZZ010000000
VERSION     DALTZZ010000177.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27908"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1244
                     /locus_tag="O0X12_03360"
     CDS             <1..1244
                     /locus_tag="O0X12_03360"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HJK53226.1"
                     /translation="MLIAAFGIIAAAIGTLFVRTKKNENSAIHKAFNTGTVIALIISV
                     IATYFLTSNLVGGQFGMGIWFATIAGLVAGFGIGLITEYYTSFDYKPTRAISESCQTG
                     AATNIINGFAKGMESTVWPVLIIAAATYVAYYFAGMYGIGIAAVGMLSTLGLTLAVDA
                     YGPVADNAGGIAEMSGQPHSVREITDTLDAVGNTTAAIGKGFAIGSAALTALALFSAY
                     ATAVGLEAIDLLNTNVFIGILIGGMLPFLFSALTMTAVGQAAQKIVIEVRRQFKEIKG
                     LMEGTAEPDYTSAIAIATDSAIHKMILPGVIAIVAPIIVGLVLGLLALGGLLVGSLVA
                     GVLLALTMANSGGAWDNAKKYIEIGNYGGKGSDAHKAAVVGDTVGDPFKDTSGPALNI
                     LIKLMSMIALVFVPVILAFAA"
     gene            complement(1425..1757)
                     /locus_tag="O0X12_03365"
     CDS             complement(1425..1757)
                     /locus_tag="O0X12_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJK53227.1"
                     /translation="MNYDPKSLIGTPVDLASLTEYQPGSVVSRMVINKKAGTVTAFAF
                     EAGEGLSEHTAPYDALVIVLEGEADIPVGGVLHHLKAGQMIIMPANVPHAVHPTTRFK
                     MMLVMIHE"
     gene            complement(1801..2283)
                     /gene="rimI"
                     /locus_tag="O0X12_03370"
     CDS             complement(1801..2283)
                     /gene="rimI"
                     /locus_tag="O0X12_03370"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HJK53228.1"
                     /translation="MVVVNGVGGTAAGCTLRKATLEDIPEIYRIELMCFSDPWDYKGM
                     IEMMGVFLTSFYVAEADGRIVGFSAGAVEETDEEKYGHICNIAVVPELRGFGIGRLLL
                     RKLERDFFLDGCCACSLEVRVSNTAAQAFYKKIGYEEVIVFGEYYADGEDAIVMMRWF
                     "
BASE COUNT          542 a          609 c          527 g          643 t
ORIGIN      
        1 cgatgcttat cgctgcattc ggtatcattg ccgctgcaat cggaacgctc tttgttagaa
       61 caaagaagaa tgagaactct gcaatccaca aggcattcaa cacgggaaca gttattgcac
      121 tgattatttc cgttattgca acctacttcc tgacctcgaa ccttgtcggc ggccagtttg
      181 gtatgggtat ctggtttgca accatcgccg gtcttgttgc aggctttggt atcggtctga
      241 tcaccgagta ctacacctcc ttcgactaca aaccgacgag ggcaatctcc gagtcctgtc
      301 agaccggagc tgcaaccaac atcatcaatg gttttgcaaa gggaatggag tcgaccgtct
      361 ggccggttct gatcattgca gccgcaacct atgttgcata ctacttcgcc ggcatgtacg
      421 gtatcggtat tgcagctgtt ggtatgctgt ctaccctcgg tctgaccctt gcagttgatg
      481 catacggtcc ggttgctgac aatgctggtg gtatcgccga gatgtccggt cagccgcact
      541 ctgtccgtga gattaccgat acgcttgatg cagtcggaaa cacgactgct gcaatcggta
      601 aaggatttgc aatcggttcc gctgcactga cagctcttgc tctcttcagc gcctacgcaa
      661 ctgcagtggg tcttgaggca atcgatctct taaacactaa tgtgttcatt ggtatcctga
      721 ttggcggtat gcttccgttc ctcttctctg cacttaccat gaccgcagtc ggtcaggcag
      781 cacagaagat tgtgattgag gttcgccgtc agttcaaaga gatcaagggc ctgatggaag
      841 gtactgcaga gcctgactac acgtctgcaa ttgctattgc aaccgactcg gcaattcaca
      901 agatgattct tccgggcgtt attgcaatcg ttgcaccgat cattgtcggt cttgttcttg
      961 gtcttcttgc acttggcggt ctccttgttg gttcccttgt tgctggtgtg cttcttgccc
     1021 ttactatggc aaactccgga ggagcttggg acaatgcaaa gaagtacatt gagattggca
     1081 actacggtgg taaaggctct gatgcccaca aggcagcagt tgtcggtgac accgttggtg
     1141 atccgttcaa ggatacatcc ggtccggctc tgaacattct gattaagctg atgagcatga
     1201 ttgccctggt gtttgtgccg gtaatcttag ctttcgctgc ataatcagag aaaaatcata
     1261 tccacatttt tttccaagac tgttttggta ctccttaata gtatgtacca gtcgtattca
     1321 aaaaagtgtg atgatggttt ttctggcaaa ataaaccagg tgttgttgaa tctttttccg
     1381 gtttgtgtac tgtttttgca acaacaaacc actaatggct tcttttattc gtgaatcatt
     1441 accagcatca tcttgaaccg ggttgtcggg tgcaccgcgt gtggtacatt tgccggcata
     1501 ataatcatct gtccggcttt caggtgatgt aaaacaccgc cgaccgggat atctgcctct
     1561 ccttcaagaa caattaccag cgcgtcatat ggtgcggtat gttcggaaag cccttcccct
     1621 gcttcaaatg caaatgcagt aacagttccc gcttttttat tgatgaccat ccgggacaca
     1681 accgatcctg gctgatactc cgtgagagag gcgagatcaa ctggagttcc gatcagggat
     1741 tttggatcat agttcatacc gagtacttgt tctctgaaaa gataaatctc tgtttcccgg
     1801 tcaaaaccac cgcatcatca caattgcatc ctcaccatcc gcgtagtact cgccgaacac
     1861 gatcacctct tcatacccga tctttttgta aaatgcctgt gcagccgtgt tacttacccg
     1921 gacctcaaga ctgcatgcac agcatccgtc cagaaaaaaa tcacgttcca gttttcggag
     1981 caaaagtctc ccaatcccaa acccccgcag ttctggaact accgcaatgt tacagatatg
     2041 gccgtacttc tcctcatccg tctcctcaac cgctcccgcg gaaaacccga cgatccgtcc
     2101 gtccgcctct gcaacataga aggatgtcag aaacactccc atcatctcaa tcataccctt
     2161 atagtcccat gggtctgaaa agcacatcag ctcgatccgg tagatctccg gaatatcctc
     2221 aagtgtcgcc ttacggagag tacagccggc ggcagttccg ccgacaccgt tcaccactac
     2281 cattgttatt ttagtattgt caccgaacta tattatagga g
//
LOCUS       DALTZZ010000178         1740 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_28370,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000178 DALTZZ010000000
VERSION     DALTZZ010000178.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28370"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="O0X12_03375"
     CDS             <1..605
                     /locus_tag="O0X12_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53229.1"
                     /translation="IWCLGAAEVSPDFYPRYAVTSRTYRYYFPYVADVAAMDAAARQF
                     VGKHDFTRFSKMEEGRDPNRTVTKANVFVSKDGCPIFEVSAKSFLWNMVRGMAGFLAS
                     VGAGVADPESVNDLLTTSGHRVHPAPAEALIFWDAECDVVFQPMRQARETMRMLGRAS
                     AAARAEMHVAEALMDETPEDIWRRRLIRAYPDLQNRSNQK"
     gene            complement(641..1225)
                     /locus_tag="O0X12_03380"
     CDS             complement(641..1225)
                     /locus_tag="O0X12_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HJK53230.1"
                     /translation="MTLITAVNGSPRKHGNTETVLDAFLRGAESTGAETNKLSLVDID
                     HKNCRGCNACHKKGVCILKDDLTPIFDAVLASDILVLASPIYSMTVTAEMKSFIDRGQ
                     FLWAQKFVTKTLAFSEEHLKHHIGVYLGTSGQEIPHIFDAAFPVVRAFFNDAGFSYTE
                     NVLFAGMDQHGGVKGWPESVAFAEAEGKRIAELI"
     gene            complement(1222..>1740)
                     /locus_tag="O0X12_03385"
     CDS             complement(1222..>1740)
                     /locus_tag="O0X12_03385"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HJK53231.1"
                     /translation="VLAEIGDRFPAEKVVLGDLAIAPCKGCGACERLGRCVQEDDFQA
                     LSQKILDADVLILATPVYSMSVCAQAKALIDRCQVFWSRKYVLHTFEEPAGNKLGLFI
                     ATAGQTRDNIFDHTVPVARFLFDVSGIKPKHTMLLLLKGLDKKNDFLEDASAVAKTKE
                     ITASLLASLEVV"
BASE COUNT          443 a          424 c          457 g          416 t
ORIGIN      
        1 acatctggtg ccttggagca gctgaggtat cacctgattt ttatccccgt tatgcggtta
       61 cttcgcgaac ctaccggtac tattttccct atgtagcaga cgttgctgca atggatgcgg
      121 cggccagaca atttgtggga aaacatgact ttactcggtt ttccaaaatg gaagagggac
      181 gcgatccgaa ccggacagta acaaaggcaa acgtgtttgt atcaaaggac gggtgcccaa
      241 tatttgaggt ttcggcaaag agttttctct ggaatatggt tcggggaatg gcaggatttc
      301 ttgcatcagt aggtgcggga gtggcagatc cggagtcagt gaacgatctc ctgacaacat
      361 ccggacaccg tgtacacccg gcacccgccg aagcgctgat cttctgggac gcagagtgtg
      421 atgtagtatt ccagccgatg cggcaggcac gggagacgat gcggatgctc gggagggctt
      481 cggctgcggc gcgggcggag atgcatgtcg cagaagcact gatggatgaa acgccggagg
      541 atatctggag aagacggctg attcgtgcat accctgacct tcaaaacaga tcaaatcaga
      601 aataattttt ttgaaaaaaa tagaattagc cggatgcaga ttagatgagt tctgcaatcc
      661 gtttaccttc cgcttcggca aatgccaccg actcaggcca gcctttcacc ccgccgtgct
      721 gatccattcc tgcaaagaga acgttctcgg tgtatgaaaa tcccgcatca ttaaagaatg
      781 cccgcacaac cgggaatgcc gcatcaaaga tgtgcggaat ctcctgcccg gacgttccca
      841 gatacacgcc gatgtgatgt ttgagatgct cctctgaaaa cgccaaggtt tttgtgacaa
      901 acttctgtgc ccaaagaaac tgtccgcgat caataaagga cttcatctct gcagttacag
      961 tcatcgagta gatgggggat gcaagcacga gtatatctga ggcaagcact gcatcaaaga
     1021 taggcgtgag atcatccttg agaatacaga cgcctttctt gtggcaggca ttacagcccc
     1081 ggcagttttt gtgatcgata tcaacaagag agagtttgtt cgtctcagct ccggtacttt
     1141 ctgccccacg gagaaaagca tcaagaaccg tctcggtgtt tccatgcttg cgtgggcttc
     1201 catttactgc ggttatcagg gtcatactac ctcaagggat gcaagaagag acgctgtgat
     1261 ctcttttgtt ttggcaactg ccgatgcatc ctccaaaaag tcgtttttct tatcaagacc
     1321 tttgagaagg agaagcattg tgtgtttcgg tttgattcca gatacgtcaa acagaaaccg
     1381 tgcgacaggt acggtgtgat caaagatgtt gtcacgtgtc tgtccggcgg ttgcgatgaa
     1441 aaggccgagt ttgtttccgg ctggttcctc gaaggtatgc agtacatatt tccgcgacca
     1501 gaacacctga cagcgatcga tcagggcttt tgcttgggca cacactgaca tggagtacac
     1561 cggtgttgcg aggatcagca catcagcatc aaggattttc tgcgaaaggg cctgaaagtc
     1621 atcctcctga acacagcggc caagccgctc acatgccccg catcctttac agggagcaat
     1681 cgccagatcg ccaagcacca ctttctctgc aggaaaacga tcacctatct cggcaagaac
//
LOCUS       DALTZZ010000179         2345 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_28409,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000179 DALTZZ010000000
VERSION     DALTZZ010000179.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28409"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            18..139
                     /gene="rrf"
                     /locus_tag="O0X12_03390"
     rRNA            18..139
                     /gene="rrf"
                     /locus_tag="O0X12_03390"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            471..1625
                     /locus_tag="O0X12_03395"
                     /pseudo
     CDS             471..1625
                     /locus_tag="O0X12_03395"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
     gene            complement(1622..2266)
                     /locus_tag="O0X12_03400"
     CDS             complement(1622..2266)
                     /locus_tag="O0X12_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HJK53232.1"
                     /translation="MRVKICGTATFADLGCAVAAGADAVGFLMGITHVTQDAVTPETA
                     AAMVATLPPFIVPVAVTHLTKPSDLIRIVELSRCTTLQIQDMVTPDDIAEVREVLPYL
                     RIMKAVHVMDESAITTTKYFSDTADAILLDTRTADRIGGTGITHDWNISAKIVKECSC
                     PVILAGGLTPENVTEAIIRVRPYAVDVHTGVKKNGVRDAERTRAFVANARTACD"
BASE COUNT          623 a          634 c          510 g          578 t
ORIGIN      
        1 gaacttggct tgggggattc tgcggccata gcagagaggt tccacccgga cccattccga
       61 acccggcagt taagctctct tacgtcggat attgtactga gatacgcgag ttctcgggaa
      121 ctattcaacg ctgcaccccc ccttcttctt attcttgatt cgattgattg agtgatttct
      181 tttattcggt tagtgtagtc attatttcag acaattcatc ttgcgcgttg tgctcctttt
      241 ttctgccata aatgtatata tgtgtgcatt gatgtttttt attggtttta aatgcacata
      301 ttataaatac ctctaagtaa caccttgatc tatgtttcca atagatggtc gtacgaccct
      361 taccgtggat gatattccca agaaatggta taacattgcc gcagatattg gttcgccaga
      421 aatactcaat cccgcaacaa tgaaaccggt aaccctctgt agagcaggaa cttgacacaa
      481 ctcaccggtt tattgacatc ccggaacccg ttcgtgaggc ttatgcgcag tttggcaggg
      541 ttacaccact gcagcgtgct tatcaccttg aacagtacct caaaactccg gcaaaaattt
      601 actttaaacg tgaagacgta agccccaccg gcagccacaa actgaacagt gctattgcgc
      661 aggcatacta caacaaaaag gatggagttg aacatcttac taccgaaacc ggagccggtc
      721 agtggggatc agcactttct ctctcctgca actacttcga tctcgactta atcgtcttca
      781 tggtcaaggt cagtttcgaa cagaaaccct tccgcagatc catcatggag acctatggag
      841 gaacagtcta tccctctccg agtaatgtaa ctgagtatgg aagatctgtt cttgccaaag
      901 atccttcata cggaggaact ctcggaaccg ctatcaccga agcagtcgaa atggcagtca
      961 aagatcagac cggtaaaacc aagtacagtc ttggcagtgt tgcaaaccat gtctgcatgc
     1021 atcagacggt gatcggtcag gaactcattg cccagatgga tgaactcgac atcgttcccg
     1081 actacatggt tggctgcatt ggaggcggaa gtaacttcgc aggtttcgtc ttccccatga
     1141 tcgaaaagaa actcaaagga aaagtagaca ccaccttcct tgcggtcgaa ccaagtgaag
     1201 ttccctctct caccaaagga gaattccgct atgactacgg tgatgccgga tgtatcacgc
     1261 cgatgtttaa gatgtacact ctcggccacg acttcactcc aaatgcactc catgcaggcg
     1321 gtcttcggta ccacggcatg aacccgctgg tctcagagct ctaccacaac aaaaccatca
     1381 gtgccgtatc ctacaacgaa acccagatat tccaggcagc agtaacattt gccagaaccg
     1441 aaggaatcat tcccgcaccc gaatcctcac atgcaattcg cggagcaatc gaacttgcac
     1501 ttgacgcaaa gaaaaagaat gaagaaaaaa ccattatctt caacctctcc ggtcacggac
     1561 ttcttgacat ggtcggctac ggaaacttcc tcaaaaactg tgaccaccca atgattgatg
     1621 tttaatcaca ggcagtcctg gcatttgcaa cgaatgcacg ggttcgttcc gcatcccgaa
     1681 caccattctt ttttacacca gtatgcacat ccacggcata cggacggacg cggatgatcg
     1741 cctcggtcac attttccggt gtcagacctc cggcaagaat caccgggcag gaacattcct
     1801 tcacaatttt tgcactgatg ttccagtcat gggtaatgcc agtccctcca atccgatccg
     1861 ccgtccttgt atcaaggaga attgcatccg ctgtatccga gaagtacttc gtcgttgtaa
     1921 tcgcactctc atccatcacg tgtacagcct tcatgatccg aagatatggc agtacctcgc
     1981 gaacctcggc aatatcatcc ggagttacca tatcctgaat ctgcaatgtt gtacagcggg
     2041 acaactccac aatacggatc agatcggacg gctttgtcag atgggttact gcaacaggta
     2101 caatgaacgg aggaagtgtt gcaaccatcg ctgccgcagt ttccggagtc accgcgtcct
     2161 gtgttacatg ggtaattccc atcagaaatc caaccgcatc cgctccagct gcaaccgcac
     2221 agccaaggtc tgcaaacgtt gctgtgccgc agatcttcac tcgcatgcgg caacctcaat
     2281 gtcgtacacc gcatgccagc tggtcggcga gtacgaacga aggacccgct cggtaatctt
     2341 tgcgc
//
LOCUS       DALTZZ010000180         6936 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_28450,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000180 DALTZZ010000000
VERSION     DALTZZ010000180.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28450"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="O0X12_03405"
     CDS             complement(<1..389)
                     /locus_tag="O0X12_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit gamma"
                     /protein_id="HJK53233.1"
                     /translation="MGLLDKFKSLLFGKGDVSEGTQDEKPVASKQEPVASKQEEPVSA
                     KIEAPVTKAEPADMKVEQKPIIQKPKEEKPVADKITVGHVHMSGCTGCLVSLADNYEG
                     LLTILDKYADLVYCLTLADVRHIPEMDV"
     gene            complement(392..874)
                     /gene="frhD"
                     /locus_tag="O0X12_03410"
     CDS             complement(392..874)
                     /gene="frhD"
                     /locus_tag="O0X12_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-reducing hydrogenase, FrhD
                     protein"
                     /protein_id="HJK53234.1"
                     /translation="MEGLFPEIIIAGVGNELFGDDGFGPAVIRALSEYELPDNVKAMD
                     VGLGGPHLVFSMIDPEETKKLIIVDCMDFGGKPGDLTKIPADLLPEGRQERYMDAHSG
                     NLLDPIGRLKGKMDIVILGCQPAYIPAPDSEDFALELSPEVQGAIPKTVNVILKEIGS
                     "
     gene            complement(883..2250)
                     /gene="frhA"
                     /locus_tag="O0X12_03415"
     CDS             complement(883..2250)
                     /gene="frhA"
                     /locus_tag="O0X12_03415"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit alpha"
                     /protein_id="HJK53235.1"
                     /translation="MSKVVEISPTTRHEGHSKLVLKVNDEGIIERGDWLSITPVRGVE
                     KLAIGKTYEQVPKIASRVCGICPIAHTLAATEAMEASMGVEIPDDAYLLRIILQCANR
                     LHSHALHDILALPDMYLPGTDSKINPFSPEEPVRSVAKRIQTLRMIGQTIGEIAGGEA
                     VHPSNPRVGGMYKNITPRARTKIYDLAKQALPLAREQMEFMIAVFENYAKRDWAEMCG
                     REVAIPKDLGFHQQGYMATDPCYGSSSLDEHPTWDPARWLEVRPWDWYMGEEEITLED
                     PTYPIGGTTKAGTKAWPQMQACTGVPLYDGQPVEVGPRARLAMYRNYDRKGAIGLNIA
                     REMEYMDSVYKIIEAADALNTSGKVVADVIPQGDGTIGWAANEAPRGADVHLAKVRNG
                     KVEWFSMLVPTTWNFPTCSRALTGAPWELAEVVVRAYDPCVSCATHMIVLDEDNRLVA
                     QKLLE"
     gene            complement(2648..3583)
                     /gene="mptA"
                     /locus_tag="O0X12_03420"
     CDS             complement(2648..3583)
                     /gene="mptA"
                     /locus_tag="O0X12_03420"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HJK53236.1"
                     /translation="MSLPDVQSTAPDVRISLTRVGVKNVKKLVEVGRPGKTRPAIFIS
                     DFDVFVDLPSSLKGANLSRNFEVIDEVLQQATSGDVRGIEDVCGIVSRKLLDHHEYAD
                     RTEVFMRSFYMINRDTPVSKTSCQEVVNVYAHAIAQRTDAEPIVRKSVGAEVTGITAC
                     PCAQNIMKDHAMHVMQKLDIPEDKIDAFFKEVPMASHNQRGRGFLCVETDDDIVVPLE
                     QIVTVLKESMSAKIYELLKRGDESYVVMAAHKNARFVEDCVREMARQVVNTFTYLPGD
                     THITIRQTNEESIHQHDAYAERKATLAELREELNV"
     gene            3714..4700
                     /gene="ilvC"
                     /locus_tag="O0X12_03425"
     CDS             3714..4700
                     /gene="ilvC"
                     /locus_tag="O0X12_03425"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HJK53237.1"
                     /translation="MLRKYYDADVDWSRIADKTVAVIGYGSQGRAQAANLRDNGLAVI
                     VGVRPGRSFEAASLEGFTTMPVADAVRKADVLAMLVPDESAAEIFRRDMLPWLHDGQT
                     IVFAHGFNIHYGQIVPPPNVDVVMVAPKGVGPQVRRLFLEDSGVASLIAVHQDVTGSA
                     KQMALAFAKGIGSARSVIMESTFADETETDLFGEQTVICGGVTHLIKAAYDTLVAAGY
                     EPEMAYYECMHELKLVVDLLYEGGFTKMHDSVSKTAGYGGLTRGPRVIGPETRAAMQE
                     VLGEIRSGVFAREWLLENQVNRPHYTSLVRLEKESELEKVGSEVRQIISAKK"
     gene            complement(4807..5181)
                     /locus_tag="O0X12_03430"
     CDS             complement(4807..5181)
                     /locus_tag="O0X12_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HJK53238.1"
                     /translation="MKFSMLHNNINVFDLDRSMKFYAEALGLFEVGRIAPETGDFIIV
                     YLGDGTSGHKLELTWLRDREEPYDLGDNEFHLAFGTDDYDGALAKHTEMGCVAFINED
                     MGIYFITDPDGYWLEILPPGRG"
     gene            5278..6771
                     /locus_tag="O0X12_03435"
     CDS             5278..6771
                     /locus_tag="O0X12_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HJK53239.1"
                     /translation="MTQDIQKTLETFFHHQTFRPNQEEIIQDVVAGRDVLAVMATGGG
                     KSLCYQLPAMMLDGMTVVISPLIALMKDQVDALSVQGVRVETLNSTQTYDERLRVEKE
                     ISAGLVRILYVSPERAVTPAFAAILSRCRVALFAVDEAHCISMWGHQFRPEYREIKTL
                     REKFPAVPVIALTATATERVREDIVRELGMKNPREYVGSFNRKNLRYEVYEEPTSSIR
                     VQRIVSYVVAHPNVSGIIYCFSRKSCEEIAERLRRSRILASPYHAGMPTPERSRIQEG
                     FLNNSIRVICATIAFGMGIDKPDVGYVIHAHLPKDLESYYQETGRAGRAGGRADCILY
                     YSSGDRAKISSMLEREYSQSEKLRIAKQKLADMYSYCVTPGCRRKVLLSYFGEEISDC
                     GNCDRCDREKVKQKKTAVPSGDLSRIVYDAASSLSGQMTVSSFVSFLLGLDRAHTKSS
                     GLTSHPFYGAAQNHERSEVMGVVQDLIAAKKLCLKGNAVMKLCRAGK"
BASE COUNT         1567 a         1713 c         1769 g         1887 t
ORIGIN      
        1 acatccatct ctgggatgtg acgcacatcg gcgagcgtca ggcagtagac gaggtcagcg
       61 tatttgtcaa ggatagtaag taatccttcg tagttatctg caagggacac gagacatccg
      121 gtacatccgc tcatgtgtac gtgtcctaca gtaatcttgt cagccactgg cttctcctcc
      181 tttggttttt gaataattgg cttttgttcg accttcatgt cagccggctc tgccttcgta
      241 acaggcgctt caatctttgc tgacacaggt tcttcctgtt tactggcaac aggctcctgt
      301 ttactggcaa caggcttttc gtcctgcgtt ccctcgctga catcgccttt tccgaacagt
      361 aagcttttga atttatctaa taatcccatc tttagcttcc tatctctttc aagatgacgt
      421 ttacagtttt gggaatggcc ccttgtactt ccggactcag ttcaagagca aagtcttcac
      481 tgtccggtgc aggtatatag gcgggctggc atccgagaat aacaatgtcc attttccctt
      541 tcagtctgcc gataggatcg agcagatttc cggagtgggc atccatgtaa cgctcctgtc
      601 tgccttccgg caggaggtcg gcggggatct tcgtcaggtc tccgggcttt cccccaaagt
      661 ccatgcagtc cacaatgatc agtttcttcg tttcttccgg atcgatcatg gaaaaaacca
      721 gatgtggacc tccgaggccc acatccattg cttttacatt gtcgggaagt tcgtactcgg
      781 aaagtgctct gatcactgcc ggaccaaatc catcgtcgcc aaacagctcg ttaccgacgc
      841 cggcgattat tatttctgga aacagtcctt ccatgtatat actcactcaa ggagcttctg
      901 tgccacaagc cggttgtctt catccagtac aatcatgtgg gttgcacagg atacacaggg
      961 gtcatatgca cggacaacaa cttctgcaag ctcccatggt gcgccggtaa gtgcacggct
     1021 gcaggtcggg aagttccagg tcgtcgggac gagcatggag aaccactcaa ctttgccgtt
     1081 gcggaccttt gcgaggtgaa cgtctgcgcc acgcggtgct tcgtttgctg cccatccgat
     1141 ggttccgtca ccctgcggaa tgacgtctgc gacgactttt ccggaggtgt tgagtgcatc
     1201 tgctgcctca atgatcttgt agacggagtc catgtattcc atttcacgtg caatgttgag
     1261 accaatggct cccttgcggt cgtagtttct gtacattgca agacgtgcac gtggtcctac
     1321 ctcaacaggc tgtccgtcgt agagcgggac accagtacat gcctgcatct gcggccatgc
     1381 ctttgtacct gctttggtgg ttccgccaat tgggtatgtc gggtcttcaa gagtgatctc
     1441 ttcttcgccc atgtaccagt cccaaggtct gacttcaagc cagcgtgctg gatcccaggt
     1501 cgggtgctcg tcaagagagc tgcttccata gcatgggtcg gttgccatgt atccctgctg
     1561 gtggaaacca aggtccttcg gaattgcgac ttcgcgaccg cacatctctg cccagtcacg
     1621 ctttgcatag ttttcgaaga ctgcaatcat gaactccatc tgctcacgtg cgagcgggag
     1681 tgcctgcttt gcaagatcat agatcttggt tcttgcacgc ggagtgatat tcttgtacat
     1741 accaccaaca cgcgggttgg acgggtgaac tgcttctcca cctgcgatct caccaatcgt
     1801 ctggccgatc atacgcagag tctggatacg ctttgcaaca gatctgaccg gctcctcagg
     1861 ggagaacggg ttgatcttcg agtcagtgcc cggcaggtac atatccggca gtgcaagaat
     1921 gtcgtgcagt gcgtgggagt gcagtctgtt tgcacactgg agaatgatac gcagaaggta
     1981 tgcgtcgtct gggatctcta cacccattga tgcttccatt gcctctgttg cggcaagagt
     2041 gtgggcaatc ggacagatac cacacacacg ggaagcgatc ttgggaacct gctcataggt
     2101 cttaccgatg gcaagcttct ctactccacg aaccggcgtg atggaaagcc agtctccgcg
     2161 ctcaatgatt ccttcatcgt ttacttttaa aacgagcttg gagtgacctt catgtctggt
     2221 ggtcggggaa atttctacta ctttcgacaa tgttatcgcc tcatcctaaa tattttaggt
     2281 tgttcgatta gctatacagg aaaattacca ttgtcctttt gggttaatga cacgtaccga
     2341 agtacgctat cttaactttc tcgcacggca tttattaaag attttctctc tttgtttcag
     2401 agaataacct ggctgtttca ggtctggaat caattggtta tacttagatc agatgaaatg
     2461 atcgtacgtg ttacttatca tctaaattgt gttacgagtg ttgaaaaaac gtttttttgc
     2521 ccttgttttt tgagagagat gccgtgtttt tttagcacta tcgagaatta tttatgcgct
     2581 ctgggaaaat tccgaaaatt gttcggataa tcttcattta atatgagaaa aaagtgtatg
     2641 ggatgtatta tacattcagc tcttcacgca gttcagcgag cgttgctttc cgctctgcat
     2701 aggcatcgtg ctggtgaata ctttcttcat tggtctggcg gatggtgata tgtgtatcac
     2761 ctggaagata ggtaaaggtg tttacgacct ggcgtgccat ttcacgaaca cagtcttcta
     2821 caaatcgtgc gttcttgtgt gctgccatga caacataact ctcgtcgcca cgcttgagaa
     2881 gttcgtaaat ttttgcactc atggactcct tgaggacagt gacgatctgt tccagaggaa
     2941 caacaatgtc gtcgtcggtt tccacgcaca ggaatccccg gccacgctgg ttgtggcttg
     3001 ccatgggaac ttccttgaaa aatgcatcaa tcttgtcttc ggggatatcg agcttctgca
     3061 tcacatgcat tgcatgatcc ttcatgatgt tctgggcaca ggggcatgca gtgataccgg
     3121 tgacctcagc tcccacactt ttccggacga ttggttctgc atctgttctc tgggcgattg
     3181 catgagcata gacatttaca acctcctggc aggaagtctt tgaaaccggg gtgtcgcggt
     3241 tgatcatata gaaactgcgc atgaacactt cggttctgtc tgcatactcg tgatgatcga
     3301 gcagttttcg tgagacgata ccacacacat cttcaatacc acggacgtcg ccggaggttg
     3361 cctgctgcag tacctcgtca atgacctcaa agttgcggga aaggtttgcg cctttcaggc
     3421 tgctggggag atcgacaaag acatcaaagt cggagatgaa aattgccgga cgtgttttgc
     3481 cgggccttcc aacttcaacg agcttcttga catttttcac accgacacga gtcaggctga
     3541 ttcgtacgtc aggagcagtg gactgcacat ctggtaatga cattatactg tatccttcgt
     3601 tcttctgccg gaaaattttg ctaaaggtac cgacagggta acgtgcatct atgtgtattg
     3661 ccccaacggt atataatttt atatgtgtct gggcctatct atcactgaca cgcatgctga
     3721 gaaaatatta tgatgctgac gttgactggt caaggattgc cgataaaact gtggcggtga
     3781 ttggttatgg atcccagggg cgcgcccagg cggcaaatct acgggacaac ggtcttgcgg
     3841 taattgtggg ggtccgtccg ggacggagtt ttgaagcagc aagcctcgaa ggatttacta
     3901 ctatgccggt tgctgatgcc gtccgtaaag cagatgttct tgccatgctg gtcccggacg
     3961 aatcagcagc ggagatcttc cgtcgtgata tgctcccgtg gctgcatgac ggccagacaa
     4021 ttgtctttgc ccatggcttc aacattcatt acggtcagat cgtacctccg ccgaatgtgg
     4081 atgtcgtgat ggtcgcaccg aaaggtgtcg gtccgcaggt aagacggctt ttccttgagg
     4141 attccggcgt tgcttctctt attgcggttc accaggacgt taccggttct gcaaagcaga
     4201 tggcgctcgc gtttgcaaaa ggtattgggt cagcccgttc ggtaatcatg gaatcaacgt
     4261 ttgccgatga gaccgagacg gatctgtttg gagaacaaac cgtgatctgc ggcggcgtta
     4321 cccatctgat taaggcagct tatgatacgc tagttgcggc aggatatgaa ccggagatgg
     4381 catattatga gtgtatgcat gaactgaaac tggtcgttga tcttctctat gaaggtggct
     4441 ttacgaagat gcatgactcc gtatcgaaga ccgctgggta cggcggtctg acccgcggtc
     4501 cgcgggtgat cggtccggag acccgtgctg cgatgcagga agttcttggg gagatccgtt
     4561 cgggagtatt tgcccgtgaa tggctcctgg aaaatcaggt aaaccgtccg cactacacat
     4621 cacttgtgcg gcttgagaaa gaatcggaac tggaaaaagt tggcagtgaa gtccgtcaga
     4681 ttatctctgc caaaaaataa tttttatggt tgtatctttt tcgccgctca atatcacaga
     4741 aaatctgtta ctgttctgac agagttgatc gtgtccgtgc ttctgttttc aaaaggggca
     4801 gtatttttat ccccgtccgg gcggaagaat ctccagccag tagccatccg gatcggtgat
     4861 gaagtagatg cccatatcct cattgatgaa tgcaacacag cccatctctg tatgttttgc
     4921 aagagctccg tcatagtcat ctgtaccaaa cgccagatgg aactcgttgt cgccgagatc
     4981 atacggctct tcgcgatccc gcagccaggt cagttccagt ttgtggccgg acgttccgtc
     5041 tccaagatac actataataa agtctcccgt ctctggggca atgcgtccca cctcgaaaag
     5101 gccgagtgct tcagcataga acttcattga ccgatccaga tcaaagacgt tgatgttgtt
     5161 gtggagcatg gaaaatttca tggaggatta ttggagtggt tatcatatct actcttgttc
     5221 ccaatcccac tagtttaata tgaactctgc cggataactt atccagaatt cagccatatg
     5281 acgcaggaca tccaaaaaac tctggaaacg ttcttccacc atcagacatt ccggcccaat
     5341 caggaggaga tcatccagga tgtggtcgcc gggcgtgatg tactggctgt catggcaacc
     5401 ggcggcggga aatccctttg ttaccagttg ccggcgatga tgctggacgg gatgacggtc
     5461 gtaatctccc ccctgattgc actcatgaag gatcaggtgg atgctctctc agtgcaggga
     5521 gtccgcgtgg agacactcaa cagtacccag acctacgatg aaaggctccg agttgaaaag
     5581 gagatttctg cagggctcgt tcgtatcctt tacgtatcgc ctgaacgtgc ggtaacccct
     5641 gcgttcgctg caatactttc ccgttgtcgt gttgcacttt ttgccgtgga tgaggctcat
     5701 tgtatctcca tgtggggaca tcagttccgt cccgagtacc gtgagatcaa aacactccgg
     5761 gaaaaatttc cggcagtacc ggttattgcc ctgaccgcga ccgcgaccga gcgtgtccgc
     5821 gaagacatcg tccgggaact tggtatgaag aatcctcgcg agtatgtcgg cagtttcaac
     5881 cggaaaaatc tccggtatga ggtgtatgag gagccgacca gctcgatccg tgttcaaagg
     5941 attgtcagct acgttgttgc ccatccgaat gtttcaggta ttatctactg tttctcccga
     6001 aaaagctgtg aggagattgc agaacgcctc aggcgttccc gcattctggc aagcccgtat
     6061 catgcgggaa tgccgacccc tgaacgcagc cgcattcagg aaggatttct caacaacagc
     6121 atccgcgtta tctgtgcaac gatagcgttc ggaatgggaa ttgacaaacc cgatgttggc
     6181 tatgtcattc atgctcatct ccccaaggat cttgaatcct attatcagga gactggtcgt
     6241 gcaggccgtg ccggtggtcg tgccgactgc attctctact actcttccgg tgatcgggca
     6301 aaaatatcct ctatgctgga gcgtgagtac tcccagtcgg agaagctgcg cattgcaaaa
     6361 cagaaactcg ctgacatgta ctcgtactgt gtaactcctg gatgccggag gaaagtttta
     6421 ctctcctact tcggtgagga gatctccgac tgcggcaact gtgaccgctg tgatcgggag
     6481 aaagtaaaac aaaaaaagac agctgtccct tccggggatc tctcccgcat cgtttacgat
     6541 gcagcaagca gcctttctgg gcagatgacg gtttcatcgt ttgtttcatt tcttctcggg
     6601 ctggatcgtg cccacaccaa aagttccggt ctgacctcgc atccgtttta cggtgctgca
     6661 caaaatcatg aacggtccga ggtcatggga gttgtgcagg atctgattgc cgcaaaaaaa
     6721 ctttgtctca aggggaatgc ggttatgaaa ctttgccgtg cggggaagta atttttcata
     6781 accgaacact tttccaacct cgagactgga tcacccggag attttcgttt ggaaaaatga
     6841 ggggatattg tctttaatgc tacccgtgtg tttctgtttc gcataacagc ggccgcatac
     6901 ttcctgcact gatacccata gtggatatgt tgtgga
//
LOCUS       DALTZZ010000181         2958 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_28612,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000181 DALTZZ010000000
VERSION     DALTZZ010000181.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28612"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(310..2358)
                     /locus_tag="O0X12_03440"
     CDS             complement(310..2358)
                     /locus_tag="O0X12_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HJK53240.1"
                     /translation="MDTETELIMSVVIVLACAIAILFVGRRLRMPFIIGYFITGIVVG
                     PAGLGLVTEEQVSLLAELGVILLMLTIGLEMSLKSLLSMKRIVLIGGTLQLVVTTVAV
                     WAVMVAVGFASNVALFIGFLVAHSSTAVIMNLYQNSGEIDTQHGKIALGLLIFQDLNV
                     IPMMLMVPILAGTSGTDLVGELISFVVGMVVLVIVLAAAIFLVPRFLTRIALTRSKEL
                     FIISIVVICFGIAWMMSLSGVSLALGAFLAGIAISESDYSHEAIGQILPFRDLLTSFF
                     FVSIGMMLNLVYVWDHLILIIAIAAVLLLGKTLINFVSVKAIGIASGAALLSAIGLSQ
                     VGEFSFILGSTGLEAGILTQDVYQVFLAITIVTMAVTPFAVNAGPAIAKKLIKPKVPG
                     ENIFGNDVSPCEVHPKEHVIIVGYGLAGQYVAKALKRVDLPYIILELNAETVNREKKQ
                     GERIVYGDATRESLLEYAGLMKARTIVITIPDTEAVKTIISTARRMNPRINIITRSRF
                     LADSEELYRLGADEIIVDEREAAIQIFRRILANEQVPQQDLDQYVKQIRNDLYDRYIE
                     TKEVPDAREAAKNGWFETIRLRAKSMDDKTAAARASVEQIHVCKNCEVAGKRLSEIHL
                     RANYGVSVIAVRRDGDAVVAPDGNTVLEEGDTAVVIGERAAITEILPLFTENRTEKEE
                     "
     gene            complement(2758..2943)
                     /locus_tag="O0X12_03445"
     CDS             complement(2758..2943)
                     /locus_tag="O0X12_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53241.1"
                     /translation="MTEELDSDTIQHELKRLEGLILILEKENAELRDRVSALELQTGQ
                     LTDRILRLGGNCSSCGV"
BASE COUNT          740 a          771 c          713 g          734 t
ORIGIN      
        1 cattatatac aattatgact tactattagt aatcaactct gaatgagttg cagcgaaaga
       61 aaaaaactac ggagatgtga taaccagaag ataagataaa agactgcaga gtaattctca
      121 cctgggttgc cggtctctga acacacatca ggatattacg ttaccacgca acaaccggcc
      181 ataatttcat ccagcacata ctattccacc acacactctc aatatgcggc agtcacctcc
      241 actcacacga ctgcccaaat aaaaatactc tccggcgggt ttttccacac cctcccgccg
      301 gaaaaaagat tattcctcct tttctgttct gttctctgta aacagcggca gtatctctgt
      361 aattgctgca cgttctccaa tcaccacagc ggtgtcacct tcctccagca cagtatttcc
      421 gtccggggca acaaccgcat caccatcgcg acggaccgca ataaccgaga caccatagtt
      481 tgcccgcaga tggatctctg acaaccgttt acctgcaacc tcgcagtttt tacagacatg
      541 aatctgttcg accgaagcac gtgccgcagc ggttttgtca tccatgctct ttgctctcag
      601 acgtattgtc tcgaaccatc cgttctttgc ggcctcgcgt gcgtccggaa cctcctttgt
      661 ttcaatgtag cggtcataga ggtcgttccg gatctgcttg acgtactgat ccagatcctg
      721 ctgcggcacc tgctcgtttg ccagaattct ccggaagatc tgaatggcag cctcacgttc
      781 gtccacgatg atctcgtctg ccccgagacg gtacagctcc tcactgtctg caaggaatct
      841 tgaacgggtg atgatgttga tccgcggatt cattctgcgt gcggtggaga tgattgtttt
      901 gaccgcttct gtgtccggaa tggtgatcac gatcgttctg gccttcatca ggcctgcgta
      961 ctcaagaagc gactccctgg tcgcgtctcc gtacacaatt ctctccccct gctttttctc
     1021 ccggttcacg gtctcggcat tgagttcaag aataatatac gggagatcca cacgtttgag
     1081 tgcctttgca acatactggc ccgcaagccc gtatcctaca atgatcacat gctcttttgg
     1141 atggacctcg cagggggaga catcattgcc aaagatattc tctcccggaa ctttgggttt
     1201 aatcagtttc tttgcaattg ccggacctgc attgaccgcg aacggtgtga ccgccatggt
     1261 tacaatggtg attgcaagga acacctgata gacatcctgc gtgagaatac ctgcttccag
     1321 tccggtcgat ccgagaatga acgagaactc tcccacctgg gaaaggccga tggcagagag
     1381 aagggcagca ccgcttgcaa taccaatcgc tttcacgctg acaaagttga tgagggtttt
     1441 tcccaggagc aggacggctg caatggcgat gatcaggatc agatgatccc acacatagac
     1501 aaggttgagc atcattccta tcgagacaaa gaagaaactt gtcagcagat cacggaacgg
     1561 cagaatctgc ccgatagcct cgtggctgta gtcggattcg gagattgcaa tgccggcaag
     1621 gaatgcgcca agtgcaaggg aaacaccgct taaggacatc atccatgcaa taccgaaaca
     1681 gatgacaaca atggagatga tgaaaagctc cttgctgcgt gtaagggcaa ttcttgtgag
     1741 gaaccgcggc accagaaaaa ttgcggcagc cagcacaatg accaaaacca ccatacccac
     1801 tacaaaactg ataagctctc caacgagatc cgtaccggag gttccggcaa gaatcggtac
     1861 catgagcatc atgggaatga cgtttaagtc ctggaaaatc aggagaccga gtgcgatctt
     1921 tccatgctgc gtgtcaatct ccccgctgtt ctgatacagg ttcatgataa ccgcagtgga
     1981 ggagtgtgca acgaggaatc ctatgaaaag tgcaacgttt gacgcaaacc cgacagctac
     2041 catgaccgcc catactgcga cggttgttac gacaagctgg agtgttccgc ctatcagcac
     2101 gatccgtttc atggacagga gacttttcag cgacatctcc agtccgatgg tcaacatcag
     2161 gagaatgaca ccgagttctg ccagtaatga tacctgctcc tcggtcacca gacccaggcc
     2221 cgccgggccg acaacgatac cggtgatgaa gtacccgata atgaacggca tacgtaagcg
     2281 tctgccgaca aagagaatgg cgatggcaca cgcaagtacg atcaccaccg acatgataag
     2341 ttctgtttct gtatccacgt gcaacactcc cgttgatttg tgtatttttc acggtttaat
     2401 gagagaatgc actacacaga caactgtgtg gagctttctg tgttatgtat tctgtcatta
     2461 caaaggaaaa tacccggagc tgatcctttc ggtggtgttt tttatgcgtg taatgggtaa
     2521 aaaatgctcg gtaaggtaca catgtgcgga aaaatagagc tgttttgtgc ctggacttta
     2581 tggccgggct tgggtgaatt atgaagcagc cttcctactc tgagtatgcg ggttcaacgt
     2641 catgacgtcg ctgaaccatc ttgaacactc tgcttttctt gccgcagggt ttgttcttgt
     2701 gttcctcctc ataggatccg acatatggac aacagcatat gggggatgtt tggcggttca
     2761 gaccccgcat gaggaacagt tgccgccaag gcgcagtatg cggtccgtga gttgtcctgt
     2821 ctggagttcc agcgcggaga cgcggtcccg cagttcggcg ttctcttttt cgaggatgag
     2881 aatcagtcct tcgaggcgtt tgagttcatg ctggatcgta tcactgtcaa gttcttcagt
     2941 catgtatctc tattttgc
//
LOCUS       DALTZZ010000182         3748 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_28677,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000182 DALTZZ010000000
VERSION     DALTZZ010000182.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28677"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            67..540
                     /locus_tag="O0X12_03450"
     CDS             67..540
                     /locus_tag="O0X12_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53242.1"
                     /translation="MNYKLVTVCVLLLAALVFVAGCVDSNDPSETPTPTPTVTPTVNP
                     DFSLTPGPTDEFPAGKDVTVQIGRDSNKPTITATYAGGKGTNQVSRIEVTLYRADDSQ
                     IITQQFGKEPKIGTELVFEGSSTPSTSDRVKVVAYYYSGEVVVVSDDLYTYKNRT"
     gene            complement(558..1235)
                     /locus_tag="O0X12_03455"
     CDS             complement(558..1235)
                     /locus_tag="O0X12_03455"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HJK53243.1"
                     /translation="MRPYVIINAAMSADGKISTKEHRQTKISGDQDFARVDRLRADCD
                     AVMVGIGTILADDSSLRLKNPDLIAERAAAGKPEQPMRVVIDSQARMPPDGDMFKKGT
                     GQRVIFVAKSAPADRIAALAKKATVITAGTNKVDLTMVLDKLGEMGVRKLMVEGGATL
                     LWSFTSQRLFDEIRIYVGALIIGGADAPTFVDGFGFTRSEGFTRLTLKDVEKIDDGLL
                     ITWLKKE"
     gene            1435..2400
                     /locus_tag="O0X12_03460"
     CDS             1435..2400
                     /locus_tag="O0X12_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HJK53244.1"
                     /translation="MPNLTVAVLAPNGYARDIGKKGTSTDITFYNLKKGHDTVTLLEP
                     TRYPDRLAPLFYVVSMAHEAIVVVEEINPMFAEWVLILDCAGISKGTIILKNYIQPSD
                     IAPLIKGTVLKVYEICDEDFITLSANLLARAAAQPEIEPKEGSVGTVPVDHHFNVRGI
                     GTVILGCVADGWIKKHDKVRVEPIGKTAQIRSVQKHDDDFAWAYTGDRVGCALKDIEA
                     EDLDRGFVLTTDPQVKSSTTITVPAELVKYWPTAVKEQMVISVGHWMQFLAARVTAVD
                     NGSDWHKPVLTIEMETPLVYKPGDKAVLHYLDGGKLRIMGTIILP"
     gene            2486..>3748
                     /locus_tag="O0X12_03465"
     CDS             2486..>3748
                     /locus_tag="O0X12_03465"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HJK53245.1"
                     /translation="MSTAEYRYHLAEFPAPNVSVRHITLTFDITETQTRVIAETTFLS
                     LVNSLSTLTLNAKNLEILKVRINGRTARYRYEGDILEVTLPHHAHRGAHITLWTETIC
                     RPTTNILEGLYYDTTPKGLPKTQITQCQQWGFQRIAPCIDDMRAKSTWTTTIIADNRY
                     TNLISNGNIKTPRAPYDKNRDIITYQNDHPMPPYLFFLGVGTWDTFTRILEYPDGKTV
                     RLELLVPQGTALENAAASLEILADSILWTYLFTGPERYESTDVRLEIYRLCKTRDKIV
                     ADNPEDPEAALTPIRRQISRLAADLIFGYQYPYEVYREISMQNSDFGGMENTGNTTII
                     QSRIVPGPEITDPAYEYLFGVKQHEFYHNLNGSSVTGDTPFSIWLNEAVTVMIEDDYL
                     SFLFGADYIRLQNILQIYTPGTGTFSLDT"
BASE COUNT          980 a         1065 c          852 g          851 t
ORIGIN      
        1 aaataaccct tttttctttc cggttcccag cgtaaattat aaacgtggac aggacgattt
       61 tagagtatga actacaaact tgtgaccgtg tgtgtcctgc ttcttgcagc tctggttttt
      121 gtagcgggat gtgttgattc caacgaccct tcagaaacac ctacaccaac accaacggta
      181 acgccgacgg tcaacccaga cttcagtctg acccccggac ccaccgatga gttcccggca
      241 ggaaaggatg taacagtaca gattggccgt gactcgaaca aaccaaccat caccgcaacc
      301 tatgccggcg gtaaaggtac aaaccaagtc agcagaatcg aggttaccct ctaccgtgcg
      361 gatgatagcc agatcattac ccagcagttt ggcaaagagc caaagatcgg aactgaactg
      421 gtctttgagg gatcctccac accctctaca agcgaccgtg taaaggttgt tgcctattac
      481 tactccggcg aggttgtggt tgtaagcgat gatctctata cctacaagaa cagaacgtaa
      541 aacaaaaaaa attattttta ttcttttttg agccaggtta tcagaaggcc gtcatcgatt
      601 ttctcaacat ctttgagagt aagacgggtg aatccttcgc tgcgggtaaa tccaaagccg
      661 tccacaaagg tgggggcatc tgcaccgccg atgatcagtg cccctacata gatgcggatc
      721 tcatcaaaga gccgctgact ggtaaatgac cacagaagcg tggccccgcc ttccaccatg
      781 agttttcgaa cacccatctc cccgagtttg tccagaacca tggtgagatc cactttgttg
      841 gttccggcag taatcacggt tgccttcttt gcaagagctg cgatacgatc tgcaggtgcg
      901 gactttgcaa caaaaatcac ccgctgaccg gtccctttct tgaacatgtc cccgtccggc
      961 ggcatccgtg cctgactgtc gatgacaaca cgcatcggct gttccggttt tccggcggca
     1021 gcacgctccg caatcagatc tggatttttg agacgaagcg aggagtcatc agcaagaatg
     1081 gttccgatac cgaccataac agcatcgcag tcagcccgca gacggtccac acgtgcgaag
     1141 tcctgatcgc ctgagatttt ggtctgccgg tgctctttgg tggatatttt tccgtcagca
     1201 ctcattgcgg cattgatgat gacgtaggga cgcatgtatg tccctttgcg tttgagggat
     1261 atatacgttg caggtttggg aaacggtggt atcaggatct aagaagaaaa aagagagaca
     1321 ccatgtatgc tactctctgt tttagaggaa cgtagtctgt tccaactgga ttttcaggta
     1381 tcgaaaacga tcattgaaag agttaatccc ccgcaacgtc caatacatac ggatatgcca
     1441 aacctgacag ttgcagtcct tgctcccaac ggatatgccc gggatatcgg gaaaaaggga
     1501 acaagcaccg atattacctt ctataacctg aaaaaaggac acgataccgt tacactgttg
     1561 gaaccaaccc gctatccgga cagacttgcc ccgctgtttt acgtggtctc catggcacac
     1621 gaggcaattg tcgtcgtgga agagatcaat cccatgtttg cggagtgggt gctgattctt
     1681 gactgtgcag gcatctccaa agggaccatc atcctgaaaa actacattca gccctcagac
     1741 attgcgccgc tgatcaaagg tacagttctt aaagtatacg agatctgtga tgaggatttc
     1801 attaccctgt ccgcaaacct tcttgcccgt gctgcagcac agccggagat cgagcccaaa
     1861 gagggatcgg tcggaacagt accggttgat caccacttca atgtgcgggg tatcggaacc
     1921 gtcattctcg gttgtgtggc agacggctgg atcaaaaagc atgacaaagt acgggttgag
     1981 ccgatcggca aaaccgcaca gatccgatca gtacagaaac acgatgatga cttcgcctgg
     2041 gcatatactg gagaccgtgt cggctgtgca ctgaaggata tcgaagccga ggatctggac
     2101 cgcggatttg ttcttacaac ggatccgcag gtgaaaagct ccacaacgat cacagtaccc
     2161 gcagagctgg tgaagtactg gcccacggca gtaaaagagc agatggtcat ctcggttggt
     2221 cactggatgc agttccttgc cgcacgggtg accgcagtgg acaatggttc tgactggcac
     2281 aaaccagtgc ttaccattga gatggaaacc ccgcttgtct acaaaccggg agacaaggcg
     2341 gttctgcact atctggacgg aggaaaactc cggattatgg gaacaattat tcttccctaa
     2401 cctttttttc tactctaaaa atagtcggcg tcctccgcac ccacacactc atattctcct
     2461 ggcaacaaac taacagacac aacacatgtc caccgccgaa taccgctatc atctcgcaga
     2521 atttcccgcg ccgaatgttt ctgtccgcca catcaccctg acctttgaca tcaccgaaac
     2581 ccaaacccgc gttatcgccg aaaccacttt tctctccctc gtaaacagtc ttagcaccct
     2641 caccctcaac gcaaaaaact tggagatcct taaagtccgc atcaatggaa gaaccgcccg
     2701 ttaccggtat gaaggcgata ttcttgaagt gacgctcccc catcacgctc accgaggtgc
     2761 acacatcaca ctatggaccg aaaccatctg ccgtccgacc accaacatcc tcgaaggact
     2821 ttactatgat acaaccccga aaggacttcc caaaacccag attacccagt gccagcagtg
     2881 ggggttccag cgcattgccc cctgtattga cgatatgagg gcaaaaagta cctggaccac
     2941 caccatcatc gcagacaacc ggtacaccaa cctcattagc aacggaaaca tcaaaacccc
     3001 gcgtgccccg tacgacaaaa accgtgacat catcacctac caaaacgatc atcccatgcc
     3061 cccgtacctg ttcttcctcg gcgttggcac ctgggatacc ttcacccgca tcctcgaata
     3121 ccctgacggt aaaacagtac gtctcgaact cctcgtaccc cagggaactg ccctcgagaa
     3181 cgccgcagca tcccttgaga tccttgccga cagcattctc tggacatatc ttttcaccgg
     3241 cccggaacgt tacgagtcca ctgacgtcag gcttgagatc taccggctct gcaaaacccg
     3301 tgacaaaatt gttgccgaca acccagaaga tccggaagct gcactgacac ccatccgccg
     3361 ccagatttcc cgtcttgcag cggatcttat cttcggatac cagtatccgt atgaggtgta
     3421 ccgtgaaatc tccatgcaga actcagactt tggcggcatg gaaaacaccg gcaacaccac
     3481 gattatccag agccgcatcg ttccgggtcc agagattacc gatcccgcgt atgagtatct
     3541 cttcggggtc aagcagcacg aattttacca caacctcaac ggatccagcg tcaccggcga
     3601 tacccccttc tccatctggc tcaatgaggc tgtcacggtt atgattgagg atgactatct
     3661 ctccttcctt tttggtgcag attacatccg cctgcaaaat atccttcaga tctatacccc
     3721 aggtaccggt accttttccc ttgatacg
//
LOCUS       DALTZZ010000183         2452 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_28719,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000183 DALTZZ010000000
VERSION     DALTZZ010000183.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28719"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="O0X12_03470"
     CDS             <1..265
                     /locus_tag="O0X12_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53246.1"
                     /translation="YDQFAIRIFGWESPDAAERTGYLLREVSHTDTAVLAEEMPELPE
                     GTWKFAAMLPKKMLHEMAVDEYYRLPEVLAGIRTIRVRDASHR"
     gene            complement(256..1029)
                     /locus_tag="O0X12_03475"
     CDS             complement(256..1029)
                     /locus_tag="O0X12_03475"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HJK53247.1"
                     /translation="MGERYWEVDAVRGISLIGMIIFHITSLMVIFHMIFTLDWYYEVC
                     RYIHLGTSIFVIISGVALVLRYRRMAGHSKREYHVAILKRGIEIFLIGIAIAVIASVA
                     IHFFVRDGNYMLFNFLQMMGISMIICIPFLWLGKWNIIPAAILIWAGFAIKSIHTGPI
                     WLLPLGVLPDGMFYPRDYFPLLPWVGVMLLGVAIGSVLYPNGVRRFHIPDAGSLGKAF
                     SLVGRHTLAIYLLHLPIIAAILYVIMVVSNALGMPFGYL"
     gene            complement(1173..1397)
                     /locus_tag="O0X12_03480"
     CDS             complement(1173..1397)
                     /locus_tag="O0X12_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53248.1"
                     /translation="MTTSAAKKAEIVAYLEQIVAIQNEGDNDVVEVRIAKLGDGIHHS
                     KELNHHEKHTAGEILKIRDWNALKKYLENM"
     gene            complement(1481..2098)
                     /locus_tag="O0X12_03485"
     CDS             complement(1481..2098)
                     /locus_tag="O0X12_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HJK53249.1"
                     /translation="MNGLLLKDWISLRTAIVLVIIVFVFCTALFVPWGVSSVAFLIGI
                     AMALLLPVNAMTLDTASRWERYVLALPLSRREILAARYQFAYLCLGVVALICGIAAVV
                     SVLFLDGYDLLGVSPLIWWTGCMGVALLILDLMLAVYFVYRMNIGILLFVVVCFVPTL
                     AVYVQDLMVPALLSPLALVLLLAGLLGLWISWRVALQAFECWDAA"
     gene            complement(2112..>2452)
                     /locus_tag="O0X12_03490"
     CDS             complement(2112..>2452)
                     /locus_tag="O0X12_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HJK53250.1"
                     /translation="MILMPGEGIFLPYFSPLMLMVLFVACGLLLGSIALPLTLKFGVE
                     KMRYIIMVIALTPVVVIFGMSFFADLSGITPTDPVLMPVIIGVLLAVTALFVYVSFWL
                     SVRIYAKKEF"
BASE COUNT          767 a          606 c          598 g          481 t
ORIGIN      
        1 gtatgatcag tttgccatac ggatctttgg atgggagtca ccggatgcag cggaacggac
       61 agggtatctc ctgcgcgaag tatcgcacac agatactgca gtacttgccg aagagatgcc
      121 ggaactacca gagggaacct ggaagtttgc agcgatgctg ccgaaaaaga tgcttcatga
      181 gatggcagtg gatgagtatt accgtctgcc ggaagtactt gcgggcatca ggaccatacg
      241 ggtacgggac gcgtctcaca gataaccaaa cggcatacca agggcattcg agacaaccat
      301 aatgacgtaa aggatagcag caatgatggg gaggtgcagg agatagatcg cgagcgtatg
      361 tctgccgaca agagagaagg ctttgccgag tgatcccgca tccggaatat ggaaacgacg
      421 gacaccattg gggtacagga ccgagccgat agcgacacca aggagcatca cacccaccca
      481 gggaaggagc gggaagtagt cacgaggata gaacatgccg tctggaagaa cgccgagcgg
      541 aagcagccag atcgggccgg tatggattga tttgatcgca aatcccgccc atatgaggat
      601 agcagcaggg atgatgttcc atttgccaag ccagagaaac gggatacaga tgatcatgga
      661 gatgcccatc atctgcagga aattgaagag catgtagttg ccgtcacgaa caaagaaatg
      721 aattgcaact gaggcaataa cagcaatggc aatcccgata aggaagattt caatgccacg
      781 cttgagaatt gccacatggt actcacgttt tgagtgtcct gccattctcc tgtaccgaag
      841 gacaagggca acaccggata tgataacaaa aatacttgtt ccaaggtgaa tgtagcggca
      901 gacctcatag taccagtcca gggtaaagat catgtgaaag attaccatca gggaagtgat
      961 gtgaaaaatg atcatcccga tgagagagat accccgaact gcatcgactt cccagtaccg
     1021 ctcgcccatt gcctcaacgt ttgtgttata tgataatggc ttttgtgctt gtgggattgg
     1081 cgtaaaaatg ctggcattca caggcaggca tgctaaccaa ggcagaacaa catcagaaaa
     1141 gagatgagat gacggatttc gaaaaaaaga gattacatat tctcaagata ctttttcagc
     1201 gcattccagt cgcgaatctt cagaatttca ccggctgtat gtttttcatg atggttcagc
     1261 tcctttgagt ggtggattcc gtcgccaagt tttgcgatac gtacctctac cacatcatta
     1321 tcgccttcat tctggattgc aacaatctgt tcaagatatg cgacaatttc tgcctttttt
     1381 gcagcgctgg ttgtcataaa aattaatcat ccctcatagg atatacacac tttgatagcg
     1441 gcagagaaag aacactggat cccaacagta ctgaaagaga tcatgccgca tcccaacatt
     1501 caaacgcctg caatgcaaca cgccatgaga tccataatcc caggagaccg gccaggagaa
     1561 gtaccagcgc taatggtgaa agcagagcag ggaccatcag atcctgtaca tagaccgcga
     1621 gggttggaac aaaacacaca acaacgaaca gcagaattcc gatgttcata cgatacacaa
     1681 aatacaccgc aagcatcaga tccagtatca gcaacgcaac acccatacac ccagtccacc
     1741 agatcagtgg cgacaccccc agcagatcat atccatccaa aaaaagtacg gatacaactg
     1801 ctgcaatacc gcagatcaag gccactaccc ccagacacaa atatgcgaac tgatatcggg
     1861 cggccagaat ttcccggcgg gagagtggca gtgcaagcac ataccgctcc caccggctgg
     1921 ctgtatccag ggtcattgcg ttgaccggga ggagaagagc catcgcgatc ccgataagaa
     1981 atgcgacaga actgacaccc cacggtacaa acagtgcagt acagaaaacg aacacgataa
     2041 tcacaagcac aatggcagtg cgcagggaaa tccagtcctt cagcagcaat ccgttcatat
     2101 gttacccaac tttagaactc cttttttgcg taaatccgaa ctgacaacca gaaggataca
     2161 tagacaaaaa gtgctgttac agcaaggaga acgccaataa taacaggcat cagtaccgga
     2221 tcagttggag ttatgcctga caggtccgca aagaatgaca tcccgaatat caccacaacc
     2281 ggtgtcagcg caatcaccat gatgatgtag cgcatcttct ccaccccgaa cttcagcgtc
     2341 agcgggagag caatgcttcc cagcagcaga ccgcaggcaa cgaacagcac catcaacatc
     2401 agcggactga agtagggaag aaaaatacct tcacccggca tcagtatcat ca
//
LOCUS       DALTZZ010000184         5339 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_28931,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000184 DALTZZ010000000
VERSION     DALTZZ010000184.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28931"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="O0X12_03495"
     CDS             <1..921
                     /locus_tag="O0X12_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJK53251.1"
                     /translation="IAVDSQDGKTTQTSDIRPYGLANPELRELPTLGSTKGQIDPERL
                     LAVSPDLILMSTSGADLAGEADQLTEKTGIPVVLFAQYDPGTKPDEFAENLRMLGKIL
                     GKEVRAEEILEYFDTIKMDLDNRTKDIPDDGKPTLYVGGVSYSGSHGFYSTQPNYLPF
                     RYLHANNAAAGADIGTSTVENAKIAKEQIVAWDPDILFVDLGTLTAAGGGSIVELSTD
                     PSYNSMTAVQTGEVYAVLPHTSMGANYETILANAYYIGKVLYPEQFADIDPAEKADEI
                     YEFVVGVPVYDQLNANVNNLSFTKLEIPLL"
     gene            932..2002
                     /locus_tag="O0X12_03500"
     CDS             932..2002
                     /locus_tag="O0X12_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HJK53252.1"
                     /translation="MNPENGSAPEHYRAYVGRKIALILGGGILAVIMFFVSVSVGSVS
                     IPIPDIIATLLGWQGTGLYNTIIWNIRIPQALTAVVAGAGLAIAGVAMQSVLRNPLAS
                     PFTLGLSNAAAFGAAIGILMFGAGTTGSSIADAVVVNNPYLTTICAFLFSMLTTVIIL
                     LVAKMRSATPETMVLAGVAISSLFSAGLMAIQYFVDDTKLASIVFWQFGDVSRSSWME
                     LGIITVVVAAAFAYILFKRWDYNAIDAGDETARGLGVNVEKTRLFGMIAASVISAVVV
                     AFLGVIGFVGLVCPHMVRRVIGDDHRFLIPGSFVCGAVLLLISDTVARTIIAPHVLPV
                     AVLTAFLGAPVFLYLIIRGRHM"
     gene            1999..2889
                     /locus_tag="O0X12_03505"
     CDS             1999..2889
                     /locus_tag="O0X12_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK53253.1"
                     /translation="MKDEYPEHKEHHGHHIHEHSHIEGYTHTHEHSHDVPHEHHGETP
                     GKAILNVEGVSFEYRSDPVLQNITVGVARGEILAILGPNGVGKSTLLKCMNLILKPKM
                     GTILLEEMDLTKMSGSEIARNVGYVAQRNESARMTVFDTVLLGRKPHLGWRVTEKDYQ
                     IVDAALKRLGLADMQLRYIDELSGGELQRVCICRAIVQEPSVLLLDEPTSALDLCRQM
                     AILRVIRDVVDHHNVATIMTMHDLNLALRFADRFLFMKDGKVYAACDRSGVTEDMIEA
                     VYGIAVDIVMHKDMPMVIPR"
     gene            complement(2911..5079)
                     /locus_tag="O0X12_03510"
     CDS             complement(2911..5079)
                     /locus_tag="O0X12_03510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HJK53254.1"
                     /translation="MSESAGDFSDILSLLKDNPRGMSVTEIAEAAHLNRNTVARYMDT
                     LLVSGQVEMRTFGKAKVFFISKRVPVSAMLNLSSEMVLLVGGDLRIIQVNEALLSFLS
                     VTSDEIIGSLIYDGPAKPLCSDMLTDQIRYAMHGEIVRGELRIFRNSREYYLDQKIYP
                     MVLADGKPGVTVVLDDVTEHVHAEAALEQSEAKFRRLVETVQDCIWSVDENSVITYIS
                     PQIAAICGYTPDEMLGKRFSDFMPSGADARFSWELTAEISRESGFTLLEFPFICKDGS
                     RIYCEFSGTPIVLDEASNMFLGYNGALRNVTDRRNAEQGVKRWKLFLDGVMDNIPGII
                     IVTDMKTDVTVYSNLAAEQFLHMTRAEIRSLPSSALLARLGSAHLIDAHDQVKAFRRR
                     INVPEDRVEVEGDVHYISARVLPMVLSADREYLLTMATDISDEVADRNRQLLTRELAF
                     ILEGISSTQEIWEPAMEMLPKISGFEAVAIYQRSIFDDYILFMSKGGGFAPAIPCDSI
                     VDRIIRKGEPAIFDKYRMGLFLEGTLSTMDHAASLILMPIVHESRTVACIVLGSTTPQ
                     SPDTVLRGIVMSTAFQISTVASRCLLQEKLQRERDRTRSYLDVAGVMLVVVRRDGIIE
                     MINRYGAKLLGYTENDLIGRNWFDTIVPAEFREERLQSFELMISGMMNVEDRVYHGPV
                     LCSDGTEKSIRWRNSLLREECGSVSGVVSSGEIISEEEKE"
BASE COUNT         1409 a         1367 c         1348 g         1215 t
ORIGIN      
        1 atcgcggttg atagtcaaga tggaaaaact acacagacat ctgacatacg gccatacgga
       61 cttgcaaatc cagaacttcg agaactgccc acactcggaa gtacaaaagg ccagatcgat
      121 cctgaacggc tacttgcagt atcaccggat ctgatcctca tgagtaccag tggagcggac
      181 ctagcgggag aagcagatca acttactgag aaaaccggga ttcctgtggt actgtttgca
      241 cagtatgatc caggtacaaa accagatgag tttgcagaaa atctccgtat gttaggaaag
      301 atattgggaa aagaagtacg ggctgaagaa atccttgaat actttgacac aatcaaaatg
      361 gatctcgaca atcgtacaaa agatattccc gatgatggaa aaccaacatt gtacgtgggg
      421 ggagtctcgt actccggatc acacggattt tattcaacac agccgaacta tctgccgttc
      481 cgctacctcc atgccaacaa tgccgccgca ggggcagaca ttggtacaag tactgttgaa
      541 aacgcaaaaa ttgccaagga acagatagtc gcatgggatc cagacatact gtttgttgac
      601 ttgggaaccc tcactgcagc cggaggggga tcgatcgtcg aactttcaac cgatccatct
      661 tacaactcaa tgaccgcggt tcagaccggt gaggtgtatg cagttcttcc ccacacctcc
      721 atgggagcaa actatgaaac aattcttgcg aacgcgtatt acatcggaaa agttctgtac
      781 ccagaacagt ttgccgacat cgatccggca gaaaaagcgg atgagatcta cgaatttgtg
      841 gtaggtgtac ctgtgtatga tcagctgaac gcaaatgtga acaacctgtc gttcacaaaa
      901 ctggagatcc ctctcctgtg atggaacaaa catgaatccg gaaaatggat ctgccccgga
      961 gcattatcgt gcctatgtgg gaagaaaaat tgcactgatt ctcggaggag gtatccttgc
     1021 agtcatcatg ttctttgtat ccgtgtcggt cggatcggta tccattccaa tccctgacat
     1081 tatcgcaaca ctgttggggt ggcagggaac tggactgtat aataccatca tctggaacat
     1141 cagaatccca caggcactga cagcggttgt tgcgggtgca ggtcttgcga ttgcaggtgt
     1201 tgccatgcag tcagttctcc gaaacccgct tgcctcacca ttcacactcg gcctctccaa
     1261 cgccgcagca tttggtgctg ctatcggtat tctgatgttt ggcgcaggaa caaccggcag
     1321 cagcatcgct gatgcggttg tcgtcaacaa tccgtacctg acaaccatct gtgcatttct
     1381 gttcagcatg ctgacaacgg tcatcattct cctggttgca aaaatgcgtt cggcaacacc
     1441 tgaaacgatg gtactcgcag gagttgcgat cagttctctc ttcagcgcag gactgatggc
     1501 catccagtac ttcgtggatg atacaaaact tgcgtccatt gtgttctggc agtttggcga
     1561 cgtatcacga tcaagctgga tggaactggg catcatcacc gtcgtggttg ccgcagcatt
     1621 cgcctacatt ctgttcaaac gctgggacta caatgcaatt gatgcaggag acgagaccgc
     1681 acgcggactc ggagtaaacg tagaaaaaac aagactcttc ggcatgatag cagcatctgt
     1741 aatcagtgcg gtggttgtgg cattcctcgg ggtcatcgga tttgtgggac tggtctgtcc
     1801 ccatatggtg cggcgggtaa tcggtgatga ccaccggttc ctgattccgg gaagttttgt
     1861 ctgcggggca gtgcttcttc tcatctcaga caccgttgca cgtaccataa tcgcaccgca
     1921 cgtcctgccg gtagcagttc tgacagcgtt tcttggagca ccggtgtttc tgtatctcat
     1981 catcaggggg cggcacatgt gaaagacgaa tacccggaac acaaagaaca tcacggccac
     2041 cacatccacg aacactcaca catcgaagga tatacgcaca cgcacgaaca ttctcatgat
     2101 gtcccgcacg aacatcacgg tgagaccccc ggaaaagcca tactcaatgt agagggagtg
     2161 agttttgagt accggtccga tccggtactg cagaacataa ctgtgggtgt tgcccgcgga
     2221 gagatcctcg caatcctcgg accgaacggt gtcggcaagt ccacactgct caagtgcatg
     2281 aacctgatcc tgaaaccaaa gatgggaacc attcttctgg aggagatgga tctcacaaag
     2341 atgtcaggat cagagattgc acgaaacgtg ggatacgttg cccagcgcaa tgaatcagca
     2401 cggatgacag tgtttgacac ggttcttctc ggaagaaagc cgcatctggg atggagagtc
     2461 acagagaagg actaccagat cgtggatgcc gcactcaagc gtcttggact ggctgatatg
     2521 cagctccgct acattgatga actatccggc ggtgagcttc agcgggtatg catctgccgg
     2581 gcaattgtcc aggaaccatc ggttcttctg ctggatgaac cgacatcagc actcgatctc
     2641 tgccggcaga tggcaattct ccgcgtcatc cgcgacgtgg tggatcatca caatgtggca
     2701 acgatcatga cgatgcatga tctcaatctt gccctgcggt ttgccgaccg gttcttgttc
     2761 atgaaagacg gaaaagtcta tgcggcatgt gacaggagcg gtgtgacaga ggatatgatc
     2821 gaggcagtgt acggtattgc ggttgacatt gtgatgcaca aagatatgcc gatggtcatt
     2881 ccgcgttaac tccctcaccg ccaagaaaaa ttactctttt tcctcttccg aaatgatttc
     2941 cccggaagag acaaccccgg agacactccc gcactcctca cggagaagcg agttgcgcca
     3001 ccggatcgat ttttccgtac cgtcactgca cagtaccggg ccatgataca cccgatcctc
     3061 gacattcatc atccctgaga tcatcagttc aaaggactga agacgttctt cccggaattc
     3121 agcagggaca attgtatcaa accagtttct gccgattaga tcgttttcgg tgtatccgag
     3181 aagttttgca ccataccggt tgatcatctc gataatgccg tcgcggcgaa cgacaaccag
     3241 cataactccc gcaacatcca gataactccg ggtacgatca cgttcccgct ggagtttttc
     3301 ttggagaaga cagcgcgaag caaccgtact aatctggaat gccgttgaca tgacaatccc
     3361 gcgaagtacc gtgtccggcg actgcggcgt ggttgaacca agaacaatac aggcaacggt
     3421 tctgctctca tgcacaatcg gcatgaggat gagggaggca gcatggtcca tggtggagag
     3481 ggttccttca aggaacagcc ccatccggta cttatcgaag attgccggtt cacctttccg
     3541 gattatgcga tccacaatcg agtcacaggg aatggccggg gcaaatccac cccctttgga
     3601 catgaagaga atataatcgt caaagatact ccgctggtat atggcaactg cttcaaatcc
     3661 gctgatcttt ggcagcatct ccattgccgg ctcccagatc tcctgcgtac tggaaatccc
     3721 ttccagaata aatgcaagct cccgcgtcaa cagctgacgg tttctgtccg caacctcatc
     3781 agagatgtca gttgccatgg tcaggagata ttcacgatcc gcggaaagga ccatcgggag
     3841 aacacgtgca gagatgtaat gaacgtcacc ctctacctcg acacgatcct ccgggacgtt
     3901 gatccgtctg cgaaaagcct tcacctgatc atgtgcatcg atcaggtggg cggaaccaag
     3961 acgagcaaga agtgcggagg agggaagact ccggatctcg gcgcgggtca tgtggagaaa
     4021 ctgctctgct gcaagattgg agtaaacggt aacatctgtt ttcatatcgg ttacgatgat
     4081 gatgcccggg atgttatcca taacaccatc gagaaacagt ttccaccgtt tgacaccctg
     4141 ctcggcgttc cgacgatccg tgacgtttct caaagctccg ttgtagccga ggaacatgtt
     4201 tgacgcttcg tcaagaacaa tcggagtccc agaaaactca cagtagatgc ggcttccgtc
     4261 tttgcagatg aaaggaaact caaggagtgt gaatccgctt tcacgagata tttctgcggt
     4321 gagttcccag gaaaaacgtg cgtcggcacc tgacggcata aagtcagaaa accgtttgcc
     4381 gagcatttcg tccggggtat aaccgcagat ggcagcaatc tgcgggctga tataggtgat
     4441 gaccgaattt tcgtccaccg accagataca gtcctgcaca gtttcaacaa gtctgcggaa
     4501 ctttgcttca ctctgttcca gagccgcctc agcgtggaca tgctcggtaa catcatcaag
     4561 gacaacggta acgcccggtt ttccatcggc gagaaccatc ggatagatct tctgatccag
     4621 atagtactcg cggctgttcc ggaatatgcg cagttcaccg cggacaattt cgccgtgcat
     4681 ggcataacgg atctgatccg tgagcatatc gctgcaaagc ggttttgccg gaccatcata
     4741 gataagggaa ccgatgatct catcggaagt tacggataag aaggagagca gtgcttcatt
     4801 cacctgaatg atacgaagat ctccgccaac cagaagcacc atctccgagg agaggttcag
     4861 catcgcggat accggaacac gtttggaaat gaagaacact tttgctttgc caaacgtacg
     4921 catctctacc tgtccggaga cgagaagcgt gtccatgtaa cgggcgacgg tgttgcggtt
     4981 gaggtgagct gcttctgcga tctccgtgac tgacattccg cgcggattat ccttgagaag
     5041 agaaagaata tcggaaaaat cgcctgcaga ctcactcatt actcattaat ttgcccttga
     5101 tgactataat gctttcttca ttgcacaacc attgtgccaa acatcaatgc cgtgcaatga
     5161 gagataattt tatatacaat acatgagaat agtatagtag cacccggtgt gggcaacacc
     5221 gggggcagac aggtctgtgg aacaaacgca aaaacgtaca gattccatgc agtcaaaagt
     5281 cgggcaaatc acaccacaga tgtccgaacc gaacggcaac acacctcaca cacctacac
//
LOCUS       DALTZZ010000185         3744 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_29220,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000185 DALTZZ010000000
VERSION     DALTZZ010000185.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29220"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="O0X12_03515"
     CDS             <1..821
                     /locus_tag="O0X12_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HJK53255.1"
                     /translation="TALLLAADGIWNKLMEVDPYWAGYCAILVNVHDIAAMGGRPVAM
                     VDVLSASNDELMEEVSRGMRDAATAFDVPVVGGHLHPDAPYNVIDVSILGVAELENII
                     YSSKAEPGNAIIVAIDLNGRIHPSAPMNWDSVTMKTPEVLRAQIGVMKDLGEHHHLTA
                     GKDISNPGVIGTLGMLIESSGVGAVVDLTAVPHPDLDALGITFEQWVRMYPGMGFIMT
                     ADQSQVDIVCKKFRAVGMAAQVIGTVTNTKRLTLVKGDSETVLFNFCAEGITNI"
     gene            818..1585
                     /gene="mtxX"
                     /locus_tag="O0X12_03520"
     CDS             818..1585
                     /gene="mtxX"
                     /locus_tag="O0X12_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker protein Mmp4/MtxX"
                     /protein_id="HJK53256.1"
                     /translation="MITIGIGCGADAWKVVQSANETATEDLHIRCYCCPGAVDDLSSG
                     PFVELAVCSLPYQHIVDDLLAGKINGAVRGTLPANETLRYLKKACGVDRLERIALLET
                     VDHQCFFLAPVGVDEGWTIQEKISLINDGRELARRFGLSEKTAVLSGGRIGDIGRHPI
                     VDKTISDAQEIAQLTGAVHGEILIEDAVRDCGVIIAPDGISGNLIFRTLTFLGHGVGH
                     GAPVVNISPVFVDSSRASADYGGSLRLAAALSTGKKH"
     gene            1757..1960
                     /locus_tag="O0X12_03525"
     CDS             1757..1960
                     /locus_tag="O0X12_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone family protein"
                     /protein_id="HJK53257.1"
                     /translation="MADLPKAAVVRLAKKAGAERVGEDAADALVAKAEAYIEEIAKKA
                     YEYAQHAGRKTIKAEDIDLATKE"
     gene            complement(2323..2667)
                     /locus_tag="O0X12_03530"
     CDS             complement(2323..2667)
                     /locus_tag="O0X12_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53258.1"
                     /translation="MKKLLLAAVLCAALVLVSAAGCIGSDPIVGTWETKPVLGIYISA
                     EFANDGTGTITLHSDLIESGESVTFAWEKTADNTYSITSSDKTLPGGTYKLSDDGKTL
                     SSLAGAVVLNKV"
     gene            complement(2729..>3744)
                     /locus_tag="O0X12_03535"
     CDS             complement(2729..>3744)
                     /locus_tag="O0X12_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HJK53259.1"
                     /translation="TAFGALIPVGILVVLMILWVTGGNPSAIGEFSFDRLIPQFDLST
                     VTFAASLILMFAGMEMAGYHARETANPRRDYPIAMFLAAAIICIVSIVGTLAVALVVP
                     ADHIGLNAGIVQTFQVIFTEFQLGWLIVPVSIMLAVGIIAQLSVWLVGPAKGMLPAAM
                     SGDLPPIFRKMNKYGMPVGVLIVQGIVSSAFALSMVLVPSINQGYWVLTAITALINAV
                     MYMFMFAAFVKLRKTKGNVTRPYKVQGGKVGMWLVAGIAYASLIFSFVVGLFPSGTYT
                     LTETVLYVIGMLAAVLLIILLPPYLLKKFRKESWKPTKQEFRDWYCEEHVCETDTPIP
                     YDD"
BASE COUNT          989 a          863 c          874 g         1018 t
ORIGIN      
        1 gtactgcact tctgcttgca gcagatggaa tctggaacaa actcatggaa gttgacccgt
       61 actgggcagg ttactgtgcc attctcgtta acgttcacga tattgctgca atggggggga
      121 gacccgttgc gatggttgat gttctctccg catcaaacga tgagcttatg gaggaagtca
      181 gccgcggtat gcgcgatgcg gcaaccgctt ttgatgttcc tgttgtcggg ggtcacctcc
      241 accccgatgc tccgtataac gtcattgatg tctcgattct tggtgtggcc gaacttgaaa
      301 acatcatcta cagcagcaaa gcagaaccgg gtaacgccat catcgttgcc attgatctca
      361 acggtcgtat ccatccctca gcacccatga actgggactc tgtcaccatg aaaactccgg
      421 aagtccttcg cgcccagata ggtgtcatga aagatcttgg tgaacaccac catcttaccg
      481 ccggaaaaga tatctccaac ccaggtgtca ttggaaccct tggaatgctc attgagtcca
      541 gtggtgttgg tgccgttgtt gatcttaccg cagtacctca ccctgatctt gatgctcttg
      601 gcatcacctt cgaacaatgg gtgcgtatgt accctggaat gggttttatc atgactgcgg
      661 atcagtccca ggttgacata gtctgtaaaa aattccgtgc agttggtatg gctgcccagg
      721 tcatcggtac tgttaccaac accaaacgac tcacgcttgt caaaggggat tctgagactg
      781 tactcttcaa tttctgtgca gagggtatca ctaatatatg atcacgattg gaatcggttg
      841 tggtgcggat gcttggaaag tcgtccaaag tgcgaatgaa accgcaaccg aggacctgca
      901 tatccgctgt tactgctgcc ccggggctgt tgacgatttg tcctccggtc cctttgttga
      961 gcttgctgtc tgctctttac cctaccaaca catcgttgat gatctccttg ctgggaagat
     1021 caatggtgct gttcgcggga ctcttcctgc caatgagaca ttgcggtatc tcaaaaaagc
     1081 ctgtggtgtc gatcgtctcg agcggatagc tcttcttgaa acggttgatc accagtgctt
     1141 tttccttgca cctgttggtg ttgatgaggg atggaccatt caggaaaaaa tctctttgat
     1201 caatgacggt cgggagcttg cccgccggtt tggtctctct gagaagaccg ctgttctttc
     1261 tggcggcaga atcggtgata ttggtagaca tcctattgtg gataaaacca tctctgatgc
     1321 ccaggagatc gcacagctta ccggtgctgt tcatggagaa attcttattg aggatgcagt
     1381 aagggactgc ggtgttatca ttgctcctga tggtatctct ggaaatctga tctttcggac
     1441 cctcacattc cttggtcatg gagtaggaca tggtgcacct gttgttaaca tatctcccgt
     1501 ttttgtggac agttcgcgcg catctgcgga ctatggagga tctctccgcc ttgcagcagc
     1561 cttgtcaact ggaaaaaagc attaaatttt gttatttttc aaatctggtc cgttaccata
     1621 attaatttca aatatcgtcc atattctttg caaaaagtca tattcggttc accaaagccg
     1681 tcaaactgag aaaaacaccc catatatgag atatatttat atagtatttt gaactatttt
     1741 ttcattggta ataattatgg cagacttacc aaaagcagca gttgtcagac ttgcaaagaa
     1801 agcaggcgca gaacgcgtcg gtgaagatgc agcagatgcc ctcgtagcaa aggcagaggc
     1861 ctacattgag gagatcgcaa agaaagcata cgagtatgca cagcacgcag gtcgtaagac
     1921 catcaaggca gaggatattg acctcgccac taaagaataa actgattttc ctgaaaaatc
     1981 aggggtgtgg aaaaatcgtt ggatcatagt ctggcgattt tgggatgctg gtatccttgg
     2041 gataccacaa taaatccttt cacgagtatt gtagatattt tttcaaaaca gagtttttaa
     2101 ggtattcgtg tagtcttttt ctgaacggtc tgtctgatac tttttgctat cctctctttt
     2161 tctgttttct gttcgttcat aggatgtatc ctgatactac atatgccata tgtaggtaga
     2221 ttcatcaatc gtctctgaac acacttttac tgatacaagc atacaaaaaa acatgcgtgt
     2281 ttcttgtaac tggataaaaa gaaaaaaatt gaaagggact gattatacct tgttcagtac
     2341 aacagctcct gccaaacttg acagggtctt tccgtcatcg gaaagtttgt aagttcctcc
     2401 cggcagggtt ttgtctgagc tggtgattga ataggtgttg tctgcggtct tttcccaggc
     2461 aaaagtaact gattcgccag attcgataag gtcactgtgg agcgtaatgg ttcctgtacc
     2521 atcgtttgca aactcagcag aaatgtagat gccaagcaca ggttttgtct cccatgtgcc
     2581 aacgattgga tcagatccaa tgcaaccggc agcagaaaca agcaccagcg ccgcacacag
     2641 aacagctgct aataatagct tcttcataat attaacctct tcgtggaaat ataaaattgt
     2701 ttcgaaaaaa agagtgatcc ggaatggatc agtcgtcgta ggggattggc gtgtctgttt
     2761 cacatacgtg ttcctcacaa taccagtcgc ggaattcctg ttttgtcggc ttccacgatt
     2821 cttttctgaa ttttttcagc aggtatggag gcaggagtat aatcaacagt actgcagcaa
     2881 gcattccaat gacatagaga acagtctcag tcagtgtgta ggttccgctt gggaacagac
     2941 ctaccacaaa ggaaaatatc agcgaggcat atgcgattcc tgcaaccagc cacatcccca
     3001 cttttccacc ctgaactttg tagggacggg taacgtttcc tttggttttg cggagtttta
     3061 cgaacgctgc aaacatgaac atgtacatca ccgcattgat caatgctgta attgctgtaa
     3121 gtacccagta tccctgatta attgagggaa ccagtaccat tgaaagagca aatgcagacg
     3181 aaacgatacc ctgtacaatc agaacaccga ccggcatgcc gtacttattc atcttacgga
     3241 agattggcgg cagatcccct gacattgctg caggcagcat accttttgca gggccaacca
     3301 gccacacgga aagctgggcg atgattccaa ctgcaagcat aatggagact ggtacaatca
     3361 gccacccaag ctggaactcg gtaaagatta cctggaatgt ctgtacaatc cctgcattta
     3421 gcccaatatg atctgcgggt actaccagtg ctactgcgag tgtacctacg atagagacaa
     3481 tacagatgat ggcggctgca aggaacattg ctattggata gtcacgtctt ggatttgctg
     3541 tctcacgggc atgatatcca gccatttcca ttccggcaaa catcagaata aggcttgctg
     3601 caaaggtaac tgtactcaga tcaaactgcg gaataagacg atcgaacgaa aactcaccaa
     3661 tagcgcttgg gtttcctccg gtgacccaga ggatcatcaa gacaaccaga ataccaaccg
     3721 ggatcaaagc accaaatgca gtac
//
LOCUS       DALTZZ010000186         1803 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_29356,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000186 DALTZZ010000000
VERSION     DALTZZ010000186.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29356"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="O0X12_03540"
     CDS             complement(<1..1350)
                     /locus_tag="O0X12_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HJK53260.1"
                     /translation="MNDQIRNISDTEWEIPRDFVPGMRVPGTFFLSHHLAEGLEEGAL
                     TQLANVATLPGILRCSLGMPDIHWGYGFPIGGVAAFDEDEGIISPGGVGFDINCGVRM
                     ITTPLTASDIPDMKGLIEDLFAAVPTGVGSKSPVRFSQNDLSDILTDGAANVVEMGYG
                     VEEDVRRCEENGAMTGAELEFVSKKARARGIPQCGTLGSGNHFLEVQAIDEIADPKTA
                     EIFGVKEGQICVMIHCGSRGLGHQVCTDHLKVLEEAVKKYNISLPDRQLACAPLHSPE
                     GEAYFGAMAASANYAWANRQIITHLVRELLEKKFGIAYEEMPLVYDVAHNVAKWEEHE
                     IPGDGKGRRRVCVHRKGATRAFGPGRNEIVQEFRDAGQPVIIPGSMGTSSYLLAGTTT
                     AMERTFGSTCHGAGRVQSRSSAKRSLTGTAVAEDLARRGIIVRAPNAAAIAEEAPDVY
                     "
     gene            complement(1354..>1803)
                     /locus_tag="O0X12_03545"
     CDS             complement(1354..>1803)
                     /locus_tag="O0X12_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HJK53261.1"
                     /translation="CGLFHGFCTPKTMTFEELEHTADVRMKITAPTLPELFAESGFAL
                     AETLYGEYQREPAAVSFPIESEGKDREEGIVNFLSELLFLTETEYLVPVSFDLCLEGS
                     VVRGTVAGVLFDRAKHAGGIGVKGISYSGLSFRETAAGYELIIIFDI"
BASE COUNT          410 a          541 c          422 g          430 t
ORIGIN      
        1 gtacacgtcc ggtgcctcct ctgcaattgc cgcggcgttt ggagcacgga caataatccc
       61 gcgtcttgca agatcctcgg ctactgccgt acctgttaaa ctccgctttg cagatgatct
      121 gctctgtact cttcctgcac cgtgacaggt actgccaaac gttcgttcca ttgcggttgt
      181 tgttcccgca agcagatacg aagatgttcc catgcttcct ggaataatca ccggctgacc
      241 tgcatcccgg aactcctgca caatctcgtt tctccccgga ccaaaggcgc gtgttgctcc
      301 cttgcggtga acacagacac ggcggcggcc ttttccgtca ccggggatct cgtgttcctc
      361 ccactttgcc acattatggg caacatcata cacgagcggc atctcctcat aggcgatacc
      421 gaacttcttt tccagaagtt ctcggacaag atgtgtaatg atctgacggt ttgcccaggc
      481 atagtttgca gaggccgcca ttgcgccaaa gtatgcttca ccctccggcg aatgcagagg
      541 ggcacaggca agctgtcggt ccggcaggga gatgttgtac tttttcaccg cctcttccag
      601 cactttcaga tgatccgtac agacctggtg gccgagtccc cgcgacccgc agtggatcat
      661 cacacagatc tgcccttcct tcacgccaaa aatctccgcc gtctttggat ctgcgatctc
      721 atcaatcgcc tgaacctcaa ggaagtgatt gccggaacca agcgttccac actgaggaat
      781 tccccgtgcc cgggcttttt tactgacaaa ctcaagctct gccccggtca tcgcaccgtt
      841 ctcttcgcac cggcggacat cctcctccac tccatatccc atctccacca catttgccgc
      901 accgtctgta agtatatcgg agagatcatt ctgactgaac cgcaccggac ttttcgatcc
      961 gactccggtg ggtaccgctg caaacagatc ctcaatgagt cctttcatat cgggaatgtc
     1021 tgatgcagtc agcggtgtgg tgatcatccg gacaccgcag ttgatgtcaa acccgacacc
     1081 gcccggagaa atgattccct catcctcgtc aaatgcggca actccgccga tgggaaatcc
     1141 gtatccccag tggatatcgg gcataccaag cgaacagcgg agaattcccg gaagtgttgc
     1201 aacatttgca agctgggtca gtgctccttc ctcaagtcct tcggcaagat gatgggagag
     1261 gaaaaaggtt cccggaaccc gcatgccggg cacaaaatcc cgcgggatct cccactcggt
     1321 atctgaaata tttcgtatct gatcgttcat ggtttagata tcaaagatga tgatgagttc
     1381 ataacctgcg gcggtttctc tgaaggacag tcctgagtat gaaatacctt tcacaccgat
     1441 gccgcccgca tgttttgctc gatcgaacaa tactcctgca acagttcccc taaccacaga
     1501 tccctccaag caaagatcaa aggagaccgg aaccagatac tctgtttcgg tgaggaacaa
     1561 cagttctgac aggaaattta cgatgccctc ctctctatct tttccctcgg attcgatagg
     1621 aaaagacaca gcagccggtt cccgctggta ctcgccgtac aaggtttcag caagtgcaaa
     1681 cccgctttcg gcaaacagtt ccggaagtgt cggtgcggta attttcatcc gcacatcggc
     1741 ggtatgctca agttcctcaa acgtcatcgt tttcggtgta cagaatccgt ggaacaagcc
     1801 gca
//
LOCUS       DALTZZ010000187         1605 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_29504,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000187 DALTZZ010000000
VERSION     DALTZZ010000187.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29504"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="O0X12_03550"
     CDS             complement(<1..903)
                     /locus_tag="O0X12_03550"
                     /inference="COORDINATES: protein motif:HMM:NF014610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HJK53262.1"
                     /translation="METNTAVMVRIGELWLKSEQVKKQFMASLMRNIRASLDAAGIEY
                     KTEEYRGRILIYGDAERIAAVVSRVFGIVDVSICTTCGNSPEEIAVAAVAVADGKLRP
                     GMRFAVRARRQHVGGFTSQELARIVADAVWDQVPDFVVDLEHPEYEIFVEAREYGGIV
                     YDSRIPGQGGLPLGTAGKAMLLLSSGIDSPVAAWLMMRRGVVISGVFMDGGRWAGPAT
                     KDLALDNARILSTWSPGKNFAVWVVDMEPFFAAMTTSCDRHYTCIFCKRFMMRVADAL
                     ASRGKFDALVSGENLGQVASQTIQN"
     gene            complement(882..>1605)
                     /locus_tag="O0X12_03555"
     CDS             complement(882..>1605)
                     /locus_tag="O0X12_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HJK53263.1"
                     /translation="MIALSGGQDSLVLLAAAKALDLSVDAATIVSEFSVPGEMERASE
                     MCRRLGVCWHPIEVSLLDNPAIRSNPVDRCYLCKQQLMYPLVVLANELGSTVCDGTHS
                     DDLPEERPGYAVLKQLGIRSPFADAGIGKSEIQALGDILDIPRIPPSSCLATRIPFGV
                     PITAERLKLIAAAETLLRDQGISGILRVRLIADEAVVEVEAWEMQKALALLGQIEDLG
                     FSHVRVAGYTAGGVERWRQTQQ"
BASE COUNT          423 a          484 c          360 g          338 t
ORIGIN      
        1 attctggatc gtctgtgatg caacctgccc aagattttcc ccggaaacaa gggcgtcaaa
       61 cttgccccgc gatgcaagtg catctgcaac ccgcatcata aaccgtttgc agaagataca
      121 cgtgtaatgt ctgtcacagg atgtagtcat cgctgcaaaa aacggttcca tatcaacaac
      181 ccagactgca aaatttttcc cgggtgacca ggtcgaaaga atgcgtgcat tatcaagtgc
      241 gagatccttt gtggcaggcc ctgcccatct tccgccgtcc atgaaaacac cggaaataac
      301 aacgccacga cgcatcatca gccatgctgc aaccggagag tcgattcccg aggaaagaag
      361 aagcattgcc tttcctgccg ttccgagagg cagaccaccc tgaccaggaa tacggctgtc
      421 atagacaatt ccaccgtact cacgtgcttc aacaaagatc tcgtactcag gatgttccaa
      481 atccaccacg aaatctggga cttgatccca gacagcatcc gcgacgatcc gcgcaagctc
      541 ctgactggtg aacccgccaa catgctgacg gcgtgcccgt accgcaaacc gcattcccgg
      601 acgcagtttt ccgtcggcca cggctaccgc cgctacagca atctcttccg gagagtttcc
      661 gcaggttgta cagatactca catccacaat gccgaagaca cgtgacacaa cagctgcaat
      721 gcgttccgca tcgccataga tcaaaatccg gccacgatac tcttcggtct tgtactcgat
      781 ccccgctgca tcgagtgacg cacgaatgtt gcgcatcagc gatgccatga actgtttctt
      841 tacctgctca ctcttcagcc agagttcccc aattctcacc attactgctg tgtttgtctc
      901 catctctcca cacctcctgc tgtgtatccg gcaacccgta catgagaaaa accaagatct
      961 tcgatctgac caagaagagc caaagctttc tgcatctccc acgcctcaac ctccacaacc
     1021 gcctcgtccg cgatgagacg tacacgaaga atcccagaga tcccctgatc acgcagaagg
     1081 gtttctgctg cggcaatcag tttcagacgt tctgcggtaa tcggtacacc aaaaggtatc
     1141 cgggtcgcaa gacatgatga cggcggaata cggggaatat caagtatatc tccaagagcc
     1201 tggatttcgg attttccaat accagcatcg gcaaacgggc tacggatccc aagctgtttc
     1261 agaaccgcat aacctggtct ctcttccgga agatcgtcag aatgcgtacc atcgcagaca
     1321 gtactcccca gctcattggc aagtacgaca agaggataca tgagctgctg tttgcagaga
     1381 taacaacggt ctacaggatt tgatcggatc gctggattgt cgagcagcga aacctcgata
     1441 ggatgccaac atactccaag ccttcggcac atctcagaag cccgttccat ctcacccgga
     1501 accgaaaact ccgacacgat cgtggcagca tcgaccgaaa ggtcaagagc ttttgctgct
     1561 gcaagaagga caagactgtc ctgaccgccg gagagtgcta tcatc
//
LOCUS       DALTZZ010000188         1701 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_29620,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000188 DALTZZ010000000
VERSION     DALTZZ010000188.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29620"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(44..1606)
                     /locus_tag="O0X12_03560"
     CDS             complement(44..1606)
                     /locus_tag="O0X12_03560"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HJK53264.1"
                     /translation="MTNDDSTVSSGHQSDRVYIFDTTLRDGEQSPGATMTLQEKVRLA
                     RQLELLGVDIIEAGFPAASPGDFEAVRAVSQTVKNCQVAGLCRCVAADIDRAWEALAD
                     AANPRIHVFVATSPIHMEYKLRKTPEQVLEMAVAGVRHAAALTKNVEFSAEDASRSEP
                     AFLAKVVEAVIDAGATTVNIPDTVGYSQPAEYGALIRYLKEHVNNIDKAVISVHCHDD
                     LGLAVANSLAAVEAGARQIECTINGIGERAGNTAMDEVVMNLNVRRDHYKCTCGIVTE
                     QIYPTARTLSHIIGMPIPANKAIAGANAFAHESGIHQDGVLKCRETYEIMDAATIGKT
                     GNDMVLGKHSGRHAIKAKVEDLGYKLTDEQIAVVADAVKKLADIKKEIFAEDVEAIIL
                     EEIFRMPDKFKLKYLQVHAGNGPIPPNAVVIMDVDGEEKQLHNFGVGPIDAVFNTIGT
                     IAGCKPDLEQFAVNAITGGTDAQGEVTVRLRRNSYSAIGRGADPDILVAAGKAYVNAL
                     NRLAKKVEENHA"
BASE COUNT          342 a          524 c          432 g          403 t
ORIGIN      
        1 cttctgatcc gtatgtgcct gaagaatctt ctctgcaatg gttctatgca tggttttcct
       61 ccaccttctt tgccagacgg ttgagcgcgt tcacataggc ttttcctgcg gcaaccagaa
      121 tatccggatc cgctccgcgg ccgatcgcag agtaactgtt tctccggagc ctaacagtaa
      181 cctcgccctg tgcatccgtt ccgcccgtaa ttgcgttcac cgcaaactgc tccagatccg
      241 gcttacaccc ggcaatggtg ccgattgtgt taaacaccgc atcaatcgga ccgacgccga
      301 agttgtgcag ctgcttctcc tcgccgtcca catccataat aacaaccgcg ttcggcggaa
      361 tcggaccgtt acctgcgtga acctggagat acttcagctt gaacttatcc ggcatccgga
      421 agatctcctc cagaatgatc gcctccacat cctcggcaaa gatctccttc ttgatatctg
      481 cgagcttctt caccgcatcc gcaaccaccg cgatctgctc atcggtcagc ttatagccga
      541 gatcctccac cttcgccttg atcgcatggc ggccggagtg tttgccgagc accatatcat
      601 ttccggtctt accgatggtg gccgcatcca taatctcata ggtctcacgg cacttcagca
      661 caccgtcctg gtgaatcccc gactcgtggg caaacgcatt tgccccggcg attgccttgt
      721 tcgccggaat cggcatacca atgatgtggg acagcgtccg ggcggtcgga tagatctgct
      781 cggtcacaat gccgcaggta catttatagt ggtcacggcg gacgttcagg ttcattacca
      841 cctcatccat cgccgtgttt ccggcacgct cgccgatacc gttaatggtg cactcgatct
      901 gccgggcgcc cgcctcaacc gccgcaagcg agtttgcgac cgcaagaccg agatcatcat
      961 ggcagtgcac cgagattacc gccttatcga tgttgttcac atgctctttg agatagcgga
     1021 taagagcacc gtactccgca ggctggctgt atcccacggt atcagggatg ttaacggtcg
     1081 tggcaccggc atcgatcacc gcctccacca cctttgccag aaatgcaggc tcgctgcggg
     1141 acgcatcctc tgctgaaaac tccacattct tcgtcagggc cgcagcatgc cggacaccgg
     1201 caaccgccat ctccagaacc tgttccggcg ttttgcggag cttgtattcc atgtgaatcg
     1261 gacttgtcgc aacgaacaca tggattctcg ggttcgccgc atccgccaga gcctcccagg
     1321 cacggtcgat atccgcggca acacagcggc acagacctgc aacctggcag ttcttcaccg
     1381 tctgggacac cgcccggacc gcctcgaaat cgcccggact tgcggcaggg aatcctgctt
     1441 cgatgatatc tacgccgagt agctcaagct ggcgggcgag acgcaccttc tcctgtaggg
     1501 tcatggttgc gccgggggat tgttcgccgt cacggagcgt tgtgtcaaag atatagacac
     1561 gatccgactg gtgtccgctg gacacggtag agtcatcgtt tgtcatgtta tttcaccgga
     1621 gacggggttg atactgtccc gtctcaattt gcagtatcat ctttgagttg aggggattta
     1681 taggtagata ctttttaaaa g
//
LOCUS       DALTZZ010000189         3401 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_29766,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000189 DALTZZ010000000
VERSION     DALTZZ010000189.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29766"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1355
                     /locus_tag="O0X12_03565"
     CDS             <1..1355
                     /locus_tag="O0X12_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HJK53265.1"
                     /translation="LGVMLPYTGLHHLLFTHLQHLLLVMTSANAPGTPMITKTSYISE
                     RMGDVAEYILSHDREIINRCDDSVVRDGYIIRLSRGLAPLRTAMDLGDKQILGVGPEL
                     NANATIYKGGFVVTSPHIGNIRNPPTVAYLEETIEKLTSLTGAKPGIIAHDLHPQFLS
                     TRLAHTLAEEWGAVLCPVQHHRAHIASVTTEEVVGIAIDGVGYGDDATIWGGEILTGS
                     PGEGYTRTGHLEQVLMPGGDLATKFPERMLYGILQDAETLSLLSERGWDDTSLRVLEQ
                     QTEKRFNCPVTTSTGRLLDAAAALLGVCRERTYDGEPSMVLEAYAARGTPQPLEVRIV
                     SGRNGADVLLTSEILREGRVMIKRGVSVPDTAATMQTALAKGIAELALRSAERIGIST
                     AALSGGVAINRSIRETIIAELADAEVRCLTNPRYPFGDGCISCGQVITAGILAKEGKL
                     "
     gene            complement(1447..1620)
                     /locus_tag="O0X12_03570"
     CDS             complement(1447..1620)
                     /locus_tag="O0X12_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HJK53266.1"
                     /translation="MAKYLCIFCAYIYDEDLGDPKHGIPAGTKYEDIPDSWKCPTCMI
                     PKSKPGLFRKIED"
     gene            complement(1659..2234)
                     /locus_tag="O0X12_03575"
     CDS             complement(1659..2234)
                     /locus_tag="O0X12_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53267.1"
                     /translation="MTKHSEYMEAFFGVELYKKFEDVLGDLENIELDLKDISKEVGRL
                     GGKLEDEDRIGTAREMRAATYESAQQVRDVRTFLDFYFTQSQELSQVILERDAYMLLY
                     QIFKWDMNDVRDLRGWIRDFNHVCKTIGYRPEDLLNMNRLTAVPVPEDVVRYPVYAVD
                     KHGYCLCGKHYDDIMYIDEIREEMEEKAKSA"
     gene            2380..>3401
                     /locus_tag="O0X12_03580"
     CDS             2380..>3401
                     /locus_tag="O0X12_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HJK53268.1"
                     /translation="MPSFDPDTIRCDFPILERLIYLDNAATSLSPRQVIEAQVTAEYN
                     YRANVGRGIHRLSRMATHQYQEARETLKKFLGGEHGTLAFTKNTTESANIVARGIHWK
                     KTDRIVTVLQDHHSNLLPWYRLKNDGLIEDIDAVTTVSGILDPAEIEACITRNTRLVA
                     LGHASNVFGTIAPVKEIADICKDKEVLLHIDGAQTAPHLPLELEKIGCDYFSFSGHKM
                     LGPMGTGGLWISPDVSGDDVPVPLCAGGGMVQSVRGTTFTTVDGPGRYEAGTPNVIGA
                     IGLAEAAQYLTRLGMKNVADHSAALARRMIEGLAEIPGVRVYTPADAPLIGTVSFTVD
                     GVHPHEV"
BASE COUNT          939 a          862 c          796 g          804 t
ORIGIN      
        1 accttggagt gatgctcccc tatacaggcc tgcatcatct gttgttcacc catttgcagc
       61 atctgctgct ggtgatgacg agtgcaaacg caccgggaac accgatgatt acgaagacct
      121 catacatttc cgagcgaatg ggagatgttg cagagtatat cctgtcacat gatcgggaga
      181 ttataaaccg gtgcgatgat tctgtggtga gggacggata cattattcgc ttgtcacggg
      241 gtcttgcgcc acttcggacg gcaatggatt tgggagataa gcagatcctt ggagtcggac
      301 cggaactaaa tgcaaacgca acaatctata agggagggtt tgtggtgaca tcaccacata
      361 tcggaaatat ccgcaatcca cctactgttg catatctgga agagacaatt gaaaagctta
      421 cgtccctgac aggagcaaaa ccggggatta ttgcccatga tctgcatcct cagtttctca
      481 gtacccggct tgcccacacc ctcgcagagg agtggggagc ggttctctgt ccggttcagc
      541 accacagggc tcacattgca tcggtgacca ccgaagaggt ggttggtatt gcaatcgatg
      601 gtgtgggtta cggtgatgat gcaacaatct ggggaggaga aattttaacg ggatctcccg
      661 gagagggata tacgagaaca ggacatctgg aacaggttct gatgccgggc ggtgatcttg
      721 caacaaagtt cccggagcga atgctgtatg gtatccttca ggatgcagaa acactatccc
      781 tgttgtcgga acgcggatgg gatgatacat cgcttcgtgt acttgaacag cagacagaga
      841 agcggttcaa ctgcccggta acaacatcaa caggccgatt actggatgcg gcagcggcac
      901 ttctcggagt ctgccgtgaa cggacctacg atggtgagcc gtcgatggtt cttgaggcat
      961 acgccgcacg cggaacccca caaccacttg aggtacggat tgtatccggc aggaacgggg
     1021 ctgatgttct gctgacctcg gaaattcttc gggagggacg ggtaatgatc aaacgcgggg
     1081 tttcggttcc cgacactgcg gctacaatgc agacagcact cgcaaaggga atcgcagaac
     1141 tcgcactccg atcggcagag aggataggca tttcaacggc tgcattatcc ggaggtgttg
     1201 ccattaaccg atcgatccgc gagacaatta ttgcggaact ggcagatgca gaagtgcgtt
     1261 gcttgaccaa tccccgatac ccgtttggtg acgggtgcat ctcctgcggc caggtaataa
     1321 cggcaggtat cctggcaaaa gaaggaaaat tgtgagcgaa ttaacgtcca gaaagcagga
     1381 cccacgtgaa aagatcgaat tgaactaaac caaaagagaa tatgttccag aaaaaaccaa
     1441 gagaagttaa tcctcgattt ttctgaaaag acctggtttg ctctttggaa tcatacaggt
     1501 tggacacttc cagctgtccg gaatatcctc atattttgtt cctgccggaa tcccatgctt
     1561 tggatcgccc agatcctcat catagatata cgcacagaaa atgcacagat attttgccat
     1621 atactcatat cacctgacag aaataaatat cctcggtttc aggcactctt tgccttttcc
     1681 tccatctcct cacggatctc atcaatatac ataatatcat catagtgctt accgcacaga
     1741 caatacccat gtttgtccac agcatacacc ggataacgga caacatcctc tggaactggc
     1801 actgcggtca ggcggttcat gttcagaaga tcctcgggac ggtagccaat cgttttacac
     1861 acatgattga agtcacgaat ccaaccacgg agatcacgca cgtcattcat atcccactta
     1921 aagatctgat acagcagcat gtacgcatca cgctcaagaa tcacctgcga taactcctgg
     1981 ctctgggtga aatagaaatc aaggaatgtt ctcacgtccc gtacctgctg tgccgactca
     2041 tatgttgcag cccgcatctc gcgtgcagta ccaatgcggt cttcatcttc cagctttcct
     2101 ccaagacgcc caacctcttt ggaaatatcc ttcagatcca actcaatatt ctccaaatcc
     2161 ccaagaacat cttcgaactt tttatacagc tcaacgccga agaatgcctc catgtattca
     2221 ctgtgcttcg tcattgtaga gatatttaac tcataattct atataaacca ttacttcgga
     2281 gatatcggca gaaataaata tagattcagg gattattacc taacatataa gataagttgt
     2341 aacctttata gagaagaaaa accaaaatca ccactactaa tgccttcctt tgatcccgac
     2401 acaatcagat gcgacttccc tatcctcgag agactaatat acctcgataa tgctgcaact
     2461 tccctctccc cacgtcaggt aattgaagca caagtaacgg cagagtacaa ttaccgcgcc
     2521 aatgtaggcc gaggtattca tcgcctctcc cgtatggcaa ctcaccaata ccaagaagca
     2581 cgcgagacgc tcaaaaaatt tctcggcggt gaacatggaa cccttgcatt cacaaaaaat
     2641 actactgaat cagcaaacat tgttgcacga gggatccatt ggaagaaaac cgatcggatc
     2701 gtcaccgttc ttcaggatca tcactcaaat ctgcttccgt ggtacaggtt aaaaaatgat
     2761 ggtctcattg aagacattga tgctgtaacc accgtctccg gaatcctcga tcccgcagag
     2821 attgaagcct gtatcaccag aaatacacgc ctcgtcgccc ttggccatgc ctcaaatgtc
     2881 tttggcacca ttgcaccagt aaaagaaatt gcagacatat gcaaagacaa agaagtactg
     2941 ctccacattg acggcgcgca aaccgcaccc caccttccac ttgaacttga aaagatagga
     3001 tgcgactatt tctcattctc cggccacaag atgctcgggc ccatgggaac cggtgggctc
     3061 tggatcagtc ccgatgtttc tggagacgat gttccagtac cactttgtgc cgggggtggg
     3121 atggtacaaa gtgtcagagg tacaaccttc actacagttg acggacctgg acgttatgaa
     3181 gcaggaactc caaacgttat tggtgcaatt ggattagccg aggctgcgca gtatctcacc
     3241 aggcttggta tgaaaaatgt tgcagaccac agtgcagcac ttgcacgccg aatgatagag
     3301 ggtcttgcgg agatccccgg tgtccgagtt tatacaccgg cagatgcacc gctcatcgga
     3361 acagtctctt ttaccgttga cggtgtacat ccccatgaag t
//
LOCUS       DALTZZ010000190         3869 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_29978,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000190 DALTZZ010000000
VERSION     DALTZZ010000190.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29978"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /locus_tag="O0X12_03585"
     CDS             complement(<1..1030)
                     /locus_tag="O0X12_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HJK53269.1"
                     /translation="MNKSIGLILGIAAFIALLLIPIDPEVLPTAARYAAAVTVLMAIF
                     WITQPIPIEATSLIPIVAFPLLGILTPAEACAPYADKVIFLFLGGFIIAMSMQRWGLH
                     KRIALKIIEFTGTSPRRLILGFMIATAFLSMWMSNTATAMMMVPIAIAIIATVLPTKV
                     YKDMPDEHKAFAGCIVLAVAYAASIGGIATLIGTPANGILVAQMQTIFPDAPAVDFFT
                     WLKFGIPLLLIFIPVTWAWLTHVAYRKMPKTLEHAKEALAAEVAALGPMSRGEKNTLA
                     VFLLTAFLWIFEKNKDFGTFTIPGLEMIFPGIDDCTVAIFGALLLFLLPVNWKKQEFT
                     MNWQWAVRI"
     gene            1171..2457
                     /gene="prf1"
                     /locus_tag="O0X12_03590"
     CDS             1171..2457
                     /gene="prf1"
                     /locus_tag="O0X12_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor aRF-1"
                     /protein_id="HJK53270.1"
                     /translation="MAEEIPQAQIEKDEARKRYEFKKALEKLEEKEGSGTELISLYIP
                     PDKQIYDVTAQLRDEYGQCANIKSKQTRTNVQSAISSILARLKYYKTPPENGMAVFCG
                     AINVGGDKTDLESIIVEPPEAIRTYSYRCSSNFELEPLKAMLDDKFVYGLLVIDRREA
                     YWGFLRGTHIEPINGTTSTVPGKQRKGGQSSIRFERLRLIAIHEFYKKVGERASEAFL
                     AEPNFFEKFKGVLIGGPMPTKEEFAEGDFLHHEVKKRIIGLFDASYTNEFGLRELVDN
                     AQDALRGVDIMDEKKEMTRFFKELIKENGCAAYGEESVRANLEAGAVDTLLLSEKLRK
                     ARLTIECGNCGYQEVKTLQVEAGKKFKDLPFGHCPKCQSPLYLADETDIIEELTSLAD
                     ISNTRVEIISDDFEEGSMLFSAFGGIAAILRYPTGM"
     gene            2463..>3869
                     /gene="argS"
                     /locus_tag="O0X12_03595"
     CDS             2463..>3869
                     /gene="argS"
                     /locus_tag="O0X12_03595"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HJK53271.1"
                     /translation="MYREYIHKVEHLLREITGENDVLLTDGGDHADIASTVAFALAKK
                     ERKNPSVIAAEIVAKITEHPDAVGLDVSAKGPYINFIFGGDYVAASVRAARHPDYGQL
                     PARTERVILEHTSANPNGPLHVGHIRNTVIGDTLVRVFRKAGYPVEAQYYLNDMGRQI
                     AIVAWGVKHLHYERQPNEKGDEFIVRHYVEANKIAKEKPEIEPEFDEMMEKIEAGDPE
                     TVKLFHDSVDICAEGIKQTLAAMNVHHDRFVRETTFLWNGDMQNVLARVEALPEAQHD
                     GLMMYLDLSDKGFGNRYVLRRSNGTSVYAARDLAFHLWKNGQCDRSIDVLGADHKLIG
                     AQLQTTLSMIGERPPEIVHFEFVSLPEGSMTTRGGVFITADELLAETKKRALEEVTIR
                     RPELDEAERERIASAVAVGAVRYDIVKVSAEKSTVFDWKEALDFERQSAPYIQYAHAR
                     ACSILEKAKAEGGFAENYT"
BASE COUNT         1047 a          890 c          998 g          934 t
ORIGIN      
        1 ggatccgtac tgcccactgc cagttcatgg taaactcctg tttcttccag ttgacaggga
       61 gcaggaacag tagaagagca ccaaaaatgg caacggtaca atcatcaatg ccggggaata
      121 tcatctcaag tcccggaatc gtgaatgttc caaagtcttt gttcttctcg aaaatccaca
      181 aaaatgctgt cagcagaaag accgcaagtg tattcttttc gccgcgtgac atcgggccaa
      241 gagctgcaac ttcagcagca agcgcttcct ttgcgtgttc aagcgtcttt ggcatcttac
      301 gatatgcaac atgcgttaac catgcccagg taaccgggat gaaaatcagc agcagcggaa
      361 ttccaaactt cagccaggta aagaaatcaa ccgcaggtgc gtccgggaaa attgtctgca
      421 tctgtgcaac gagaattccg tttgcaggag ttccgatcag tgtcgcaatt cctccgatac
      481 ttgccgcata ggcaactgca agaacaatac atccggcaaa tgctttgtgc tcgtcgggca
      541 tatctttgta aaccttggtt ggaagcaccg ttgcaatgat cgcaattgcg attggcacca
      601 tcatcattgc ggtagccgta ttggacatcc acattgacaa aaatgcagtt gcgatcataa
      661 acccaagaat gaggcggcgc gggctggtcc cggtaaattc gataatcttt agtgcaatcc
      721 gtttatgcag accccagcgc tgcatcgaca ttgcaataat aaaaccgccg aggaagagga
      781 agatgacttt gtccgcatac ggcgcacagg cctctgcggg agtgagaatc cccaggagcg
      841 ggaacgcaac aattgggata agggaagttg cttcaatagg aatgggctgg gtgatccaga
      901 aaatagccat cagcaccgta actgccgcag catatcgtgc agcggttggg agaacctcag
      961 gatctatcgg gattaacaac aaggcaatga atgccgcaat gccaagtatc agcccaatac
     1021 tcttgttcat aacttagatc ttacgcatga cacaaatttg agtgttcctt tttgtatcgg
     1081 gtggttgcta cctcatcatt aatcaggttg cccgaccaat ggtacaccac attctataaa
     1141 cgaataagat tttgaggatt tgtatataca atggcagagg aaatacccca ggcacaaatt
     1201 gaaaaggacg aagcgcgaaa gcgatatgaa ttcaaaaaag cactggaaaa gcttgaggaa
     1261 aaagaaggat ccggtactga gttaatttcg ctgtatattc cgccggacaa acagatctat
     1321 gacgtgactg cccagctgag agatgagtat ggtcagtgcg ctaacattaa gagcaaacag
     1381 acgcggacaa acgtacaaag tgcgatctcc tcaattcttg cacggctcaa atactataag
     1441 acgccgccgg aaaacggaat ggcagtcttt tgcggagcaa tcaacgttgg tggagataaa
     1501 accgatctcg aatccattat tgttgagccg ccggaagcca tacgtaccta cagttaccgg
     1561 tgcagttcca actttgaact tgagccgctt aaggcgatgc ttgatgacaa gttcgtttat
     1621 ggtctgctgg tcatcgaccg ccgtgaagca tactggggtt tcctgcgcgg aacccacatc
     1681 gagccgatca atggtacaac ttcgaccgtt cccggaaagc agcggaaggg tggtcagtct
     1741 agtattcgtt tcgaacgtct ccggcttatt gcaatccatg agttctacaa aaaagtcggt
     1801 gagcgcgcat ctgaggcatt tttggcagag ccaaacttct ttgaaaagtt taagggagtt
     1861 ttaattggtg ggccgatgcc gaccaaagag gagtttgctg agggagactt cctccaccac
     1921 gaggtaaaga aacgtatcat cggtctcttt gatgcatcct acaccaatga gttcggtctt
     1981 cgtgaactgg tggataatgc ccaggatgcg ctccgcggcg ttgacatcat ggacgaaaag
     2041 aaagagatga cacggttctt caaagagctc atcaaagaga atggatgtgc agcatatggt
     2101 gaggaaagtg ttcgtgcgaa ccttgaggcg ggagccgttg atactcttct tctttcggag
     2161 aagttacgca aagcccggct taccattgaa tgtggtaact gcggttatca ggaagtaaag
     2221 actctgcagg ttgaagcagg gaaaaagttc aaggatcttc cgtttggtca ctgtccaaaa
     2281 tgtcagtctc cgctttatct tgcagatgag acggatatca tcgaggagtt gaccagcctt
     2341 gcggatatct ccaacacccg tgttgaaatc atctctgatg acttcgagga gggttccatg
     2401 ctctttagtg catttggtgg tattgccgca atcctgcgct atccaacggg gatgtaacaa
     2461 tcatgtatcg ggaatatatt cacaaagttg aacatcttct ccgtgagatc acgggagaaa
     2521 atgatgtact gctcacagac ggcggagatc atgcagacat tgcctcaacg gttgcatttg
     2581 ccctggcaaa aaaagaacgg aagaacccca gtgtcattgc agctgagatt gttgccaaga
     2641 ttactgagca tccggatgct gttggtcttg acgtctcggc aaaaggccca tacatcaatt
     2701 tcatctttgg aggggattat gtagctgcat ctgttcgtgc tgctcggcat ccggattacg
     2761 gtcagcttcc ggcaagaacc gaacgggtta ttcttgaaca taccagtgcc aacccgaatg
     2821 gtcctttgca tgttggtcat atcagaaata cggtcattgg cgatacgctg gtccgggtgt
     2881 tccgtaaggc cgggtaccca gttgaagcgc agtattacct gaatgatatg gggcgccaga
     2941 ttgcaattgt tgcgtggggt gttaaacacc tccactatga gcgacagcca aatgaaaagg
     3001 gcgacgagtt cattgtgcgc cattatgtcg aggcaaacaa aattgcaaag gaaaagccgg
     3061 agattgaacc tgagtttgat gagatgatgg aaaagatcga agccggcgat ccggaaacgg
     3121 tcaaactttt ccacgactca gtggatattt gtgccgaagg tatcaaacag actcttgctg
     3181 cgatgaacgt tcatcacgat cggttcgttc gcgagaccac tttcctctgg aatggtgaca
     3241 tgcagaatgt tcttgcaaga gttgaagctc ttcccgaggc ccaacacgat gggctgatga
     3301 tgtaccttga tctttcggac aaaggtttcg ggaaccgcta tgtactgcgc agaagcaacg
     3361 ggacttctgt ttatgcagca cgcgaccttg cattccatct ctggaagaac ggtcagtgtg
     3421 ataggagtat tgacgtcctt ggtgccgatc acaaactgat cggtgcacag ctccagacga
     3481 cactttccat gattggtgag cgtcctcctg agattgtaca ctttgagttt gtctcccttc
     3541 cggaagggtc gatgaccacc cgtggtggtg tgttcatcac tgcagatgag ctccttgctg
     3601 agaccaaaaa gcgggcactt gaggaagtta ccatccgccg ccccgaactt gatgaggccg
     3661 agcgtgaacg gattgcatct gctgttgcgg tcggagccgt gcggtatgat atcgttaaag
     3721 tctctgctga aaaatcaaca gtctttgact ggaaagaagc acttgatttt gaacgacaga
     3781 gtgcacccta tatccagtat gcacacgccc gtgcctgctc tatccttgag aaggcaaaag
     3841 ccgagggtgg gtttgcagag aactatacc
//
LOCUS       DALTZZ010000191         2342 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_30024,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000191 DALTZZ010000000
VERSION     DALTZZ010000191.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30024"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="O0X12_03600"
     CDS             complement(<1..275)
                     /locus_tag="O0X12_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJK53272.1"
                     /translation="MRPMRRKAAQLHDSDAREILQTGIWGTLATADSGGQPYAVPMNY
                     AVMKDTVYLHSAQEGHKLENLRENPLVSFSVVGKAEVLADTFDMAYRS"
     gene            complement(332..937)
                     /locus_tag="O0X12_03605"
     CDS             complement(332..937)
                     /locus_tag="O0X12_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HJK53273.1"
                     /translation="MQYFSYGTTETNALKEADPILGAAIDRLGHPNRPLMPDIFTALV
                     SSVIAQQISNKAAETVQGRVTDRCGGEVTPDRIAKLTEEEIQSLGMSRRKAGYIRGIA
                     LAFVSGQINPQTLRKADENQIVRELTALPGIGVWTAEMLMLHSLQRPDICSWHDLGIR
                     RGMMRLYGLDTLTKAEFDEHRRRYSPYGSVASIYLWALAHE"
     gene            1066..1695
                     /locus_tag="O0X12_03610"
     CDS             1066..1695
                     /locus_tag="O0X12_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HJK53274.1"
                     /translation="MGLKSWVVPQDKFFFELFEEQAEIVCEAADLLVNIFADYTNIQE
                     KYRQMKDLEHRGDAMAHRAYDELNRTFITPIEPEEISRLVTALDDVIDFIDSGANMLM
                     IYEIEKPDAFMVEFASVIRKAAEEIRTGVAGLRTLNDPETIKGCCIEINRLENQGDDI
                     LSNALCSLFKTKDPVEIIKFKDIYEHFEIATDKCEDVADVFAAILIRHT"
     gene            1701..>2342
                     /locus_tag="O0X12_03615"
     CDS             1701..>2342
                     /locus_tag="O0X12_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HJK53275.1"
                     /translation="MDANTVIIIAGIVIALAFNFSNGRNDASNTVATVIATRALAPRT
                     AILIASICCLAGPFIYSTAVAKTIGKGIVDPGIFTPVLLFIGLVGAVIWVNACSHYGI
                     PVSSSHALVGGLMGAGLAAGGISAVFWPAPELVGWTILYLALGAVLGAIVMVILALIF
                     KDSLKFYAVLGAAVGAIIAIPTLMILDLFVIQGLLAIVLFICVSPMLGLIVSYI"
BASE COUNT          535 a          575 c          581 g          651 t
ORIGIN      
        1 gaccggtatg ccatgtcaaa tgtatctgca agaacctctg cttttccgac aactgaaaag
       61 gacaccagag ggttttctcg taaattttcc agtttgtggc cttcctgtgc tgagtggagg
      121 taaaccgtat ctttcatcac cgcataattc atcggaaccg catagggttg tcctccgctg
      181 tctgccgttg caagtgtgcc ccagatccct gtttgaagta tttcccgtgc gtcgctgtca
      241 tgcagctgtg ctgctttcct cctcataggc cgcatgaata tctgataggg cgggaggata
      301 attaataccg ctgagcaggt tccagacaag gttattcatg tgcgagtgcc cagaggtaga
      361 ttgacgcaac gcttccatat ggtgagtaac gacggcggtg ttcgtcaaac tctgctttcg
      421 tcagtgtatc cagtccgtac agccgcatca tcccccgccg tattccaaga tcgtgccaac
      481 tgcagatgtc cggccgttgt aacgagtgga gcatcagcat ctcagctgtc cagacaccaa
      541 ttcccggtag cgctgtgagt tccctgacga tctgattttc atcagctttc cggagtgttt
      601 ggggattgat ctgcccggaa acaaaagcaa gagcgatacc ccggatatat cctgcctttc
      661 tcctcgacat cccaagcgac tgaatctcct cttcggtaag tttcgcaatt cgatccgggg
      721 ttacctctcc gccgcaccgg tcagtgaccc tcccctgcac agtctctgct gccttgtttg
      781 agatctgctg ggcaatcaca ctgcttacaa gagctgtaaa gatatccggc atcagcggtc
      841 ggttggggtg accaagacgg tcaattgcag ctccaagaat gggatctgct tctttcagtg
      901 catttgtttc cgttgtgccg taagagaagt actgcattgt tttgtactcc gtcagatatg
      961 gacgcagatc tcataagtag tatttggctt tggaaaatac cttcttcaaa ggcaaagatt
     1021 catgtaacat accttaccat gtatctgtaa agaggttacg agaggatggg gttgaaaagc
     1081 tgggtggtac cacaggacaa attctttttt gaactgttcg aagagcaggc ggagatcgtc
     1141 tgtgaagcag cagatctctt agtgaacatc tttgccgact atacaaatat ccaggaaaaa
     1201 taccggcaga tgaaggatct tgagcaccgc ggcgatgcca tggcacaccg tgcttatgac
     1261 gaactgaacc ggacatttat cacgcccatc gagccggaag agatctcccg gctggtgacc
     1321 gcacttgatg atgtgattga cttcatcgat tcgggcgcca atatgcttat gatctacgag
     1381 atcgaaaagc ccgatgcctt catggttgag tttgcttcgg ttatccgaaa agcagcagag
     1441 gagatccgca ccggtgttgc cggacttcga accttaaacg atcccgagac catcaaagga
     1501 tgctgtattg agattaaccg tctggaaaac cagggggatg acatcctctc gaatgccctg
     1561 tgcagtctgt tcaaaacaaa agatcctgtc gagatcatta agttcaaaga tatttacgag
     1621 cattttgaga ttgcaaccga caaatgtgaa gatgtggccg atgtctttgc cgcaattctg
     1681 attcgccaca cgtgagttca atggacgcaa acacggttat tatcatcgca ggcattgtga
     1741 tcgcgcttgc gttcaacttt tcgaacggaa gaaacgacgc atccaacaca gtggcaaccg
     1801 tcattgcaac acgtgcactc gcaccccgta ctgcaattct gattgcgtcc atctgctgtc
     1861 ttgcaggacc ctttatctac tcaacagccg ttgcaaaaac cattggaaaa ggtatcgttg
     1921 atccgggtat ttttacaccg gtactgctat ttatcggtct tgtcggtgcg gtaatctggg
     1981 taaatgcctg ttcgcattac ggaatcccgg tttcctcctc acatgctctt gttggaggtt
     2041 tgatgggtgc aggtcttgca gcaggaggaa tttccgctgt gttctggccg gcaccggagc
     2101 ttgttggctg gacgattctg tatcttgcct taggtgcagt cttaggagcc attgtcatgg
     2161 tgattcttgc cttgatcttt aaggactccc tgaagtttta tgctgttctt ggtgctgcgg
     2221 tcggggcgat cattgcaatt cctactttga tgattctgga tctgtttgtc atccaggggc
     2281 tgcttgccat agttctgttt atctgtgtct caccgatgct tggcctgatt gtctcctata
     2341 tc
//
LOCUS       DALTZZ010000192         3075 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_30100,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000192 DALTZZ010000000
VERSION     DALTZZ010000192.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30100"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /locus_tag="O0X12_03620"
     CDS             complement(<1..709)
                     /locus_tag="O0X12_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HJK53276.1"
                     /translation="MYFDNIPTIPTADELLDRSFRRAAKKMREKNNKNRANEDFVRAI
                     TQATHDKLVSIIQSFPEFEQLPPFYRDLCDILFGMDNLRQALGMVGWAAKNVRDVGGE
                     ISRGMRRADPLTERKRAVARIASIVHRADDALRYLNDVRNVLRKLPVISTDEFTIVVA
                     GYPNVGKSSFIRLVSSAEPEIASYPFTTKGVIVGHRNAERRKRIQFIDTPGLLDRTDD
                     ERNAIEKQALNALVYVAD"
     gene            763..1641
                     /locus_tag="O0X12_03625"
     CDS             763..1641
                     /locus_tag="O0X12_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HJK53277.1"
                     /translation="MDLSLIQKDILITLISLYHQYSHPIKGEDIADIIKRNPGTVRNQ
                     MQALKALGLVDGVPGPKGGYHPTSRAYSELNVTADDSDSEVAILRNGESVDGVRVNEI
                     DFTTLTHADLCHALIKVIGNVRVFEVGDKVTIGPTPVNKLLIKGEVFGKDEINSALLI
                     STSEMTSLPKLPIREYMTTPLVSLEADMTVAYVMKTLLQRRIHGAPVIEGSRLLGIVT
                     QTDIVSAIDRGVPMDGQIGDIMVRNVVTIPGDMRLYEVIRRFKEQDIGRVIVTEEGKP
                     VGILTHSDIIRVFPTL"
     gene            1703..1954
                     /locus_tag="O0X12_03630"
     CDS             1703..1954
                     /locus_tag="O0X12_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HJK53278.1"
                     /translation="MKSEQFTHSIRRKRVMSTDGKIIGQIKNITFDSATGQLLNLIVR
                     PDQTFDASGYKLDGDAITLPFEAVKDITDFIVVDRYMLR"
     gene            complement(2010..2693)
                     /locus_tag="O0X12_03635"
     CDS             complement(2010..2693)
                     /locus_tag="O0X12_03635"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HJK53279.1"
                     /translation="MPKAFITDIDGTLTDDRRRLSMHAVEEIRRLVDAGIPVILASGN
                     TVCFIDALSKMIGTSGDIIAENGGVYRHSYTGMRHAEGDRSICLAAYQKIVEHFQPKG
                     EELRLYSNEYRYSDVAFARDVEAEDVSRLLAGMPVQVIDTGFAIHLQPLGLSKGAALA
                     KLAELMGLTPSDFLAAGDSINDVSMLNAAGIAVVPANASPEARAAADHIMDKPFGEGT
                     AEALAQYFP"
     gene            complement(2796..>3075)
                     /locus_tag="O0X12_03640"
     CDS             complement(2796..>3075)
                     /locus_tag="O0X12_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53280.1"
                     /translation="PYGVPFICAFWCLQRVPYVRVMGTVKWKTLSNPKDPFPENYYNK
                     TLYSFVIVHALRPSPHSVRGDLGRYYTTPHNVVVWLIIIPKPHNDDEP"
BASE COUNT          663 a          723 c          867 g          822 t
ORIGIN      
        1 gatctgcaac atacaccagc gcattgagtg cctgtttttc gattgcgttc cgctcatcat
       61 cggtccggtc cagaagacct ggcgtgtcga tgaactgaat ccgttttctc cgctccgcat
      121 tccggtggcc gacgatgaca ccttttgtgg tgaagggata agatgcaatc tccggttctg
      181 cactggagac aagtctgata aaggacgact tgccgacgtt cgggtacccg gcaaccacga
      241 tggtgaactc atctgtgctg atgaccggaa gtttgcgcag aacatttcgc acatcgttta
      301 agtagcggag tgcatcatcc gcacggtgaa cgatggatgc aatccgggcg accgcacgct
      361 tgcgttcggt taaggggtcg gcccgccgca tgccgcggga gatctctccg ccgacgtccc
      421 gcacattctt cgccgcccag cccaccatgc cgagcgcttg gcggagattg tccatcccga
      481 aaaggatgtc gcagaggtca cggtagaacg gcggaagctg ttcaaactcc gggaagctct
      541 ggatgatgct taccagtttg tcatgagttg cctgggtgat tgcccgtacg aagtcctcgt
      601 ttgcgcggtt tttattattt ttttcccgca tcttttttgc ggcacgccgg aaactgcggt
      661 ccagcagttc gtcggcggtt ggtattgtgg gaatattgtc aaagtacata gttgcctgaa
      721 tcactcgatt tctttattag ggtggcagtc tgaacaatca gtatggattt gtcgctgatc
      781 caaaaagata tccttattac acttatctcg ctttatcatc aatattcgca tccgataaaa
      841 ggggaagata tcgccgatat tatcaagcgt aaccccggta cggttcgcaa tcagatgcag
      901 gccctcaaag ctctgggtct ggttgacggc gtcccggggc cgaaaggggg gtatcacccc
      961 acctcccgcg cctacagcga actcaatgtt actgcggatg attcggacag cgaggttgca
     1021 attctccgca acggggagtc tgtggacggc gtgcgcgtga atgagatcga ttttaccacc
     1081 ctgacccacg ccgatctctg tcatgctctg atcaaggtga tcggcaatgt ccgggttttt
     1141 gaggttggcg acaaagtgac catcggtccc acaccggtga acaagctgct tatcaaaggg
     1201 gaggtgttcg gaaaagatga gataaacagt gctcttctca tctcaacgtc ggagatgaca
     1261 tctctgccga agcttccgat ccgcgagtac atgacaaccc cgcttgtttc tcttgaggcg
     1321 gatatgacgg ttgcctatgt gatgaagacg cttttgcagc ggaggattca cggtgcgccg
     1381 gtgattgagg ggtcgcggct tttggggatt gttacgcaga cggatattgt ttccgcgatc
     1441 gatcgcggtg ttccaatgga cgggcagatt ggggatatta tggtccgcaa tgttgtcacc
     1501 atccccgggg atatgcggct gtatgaggtt atccgcaggt tcaaggagca ggatatcggg
     1561 cgggtgattg tgaccgagga gggtaagccg gttggtattc ttacccattc tgatattatc
     1621 cgggtgtttc ccaccctttg aaccgtctgt tttattcaaa acggatatat acttttgtta
     1681 tcaatagatt ctactgatta ctatgaagag tgagcagttt acccattcta tccgcagaaa
     1741 gagagtgatg agcaccgacg gaaagattat cggacagatc aagaacatca cttttgacag
     1801 tgcaaccggg cagctgctga atctgatcgt gcgtccggat cagacgtttg atgcgtcggg
     1861 ctacaagctt gacggcgatg caatcactct gccgtttgag gcggtcaagg atattacgga
     1921 ctttatcgtg gttgaccgct atatgctgcg gtaaaaatag ttacaggttc tcctgccggg
     1981 gcaggggaat actcttcaga atcttttttt cagggaaagt attgggcaag agcttcggct
     2041 gttccttctc cgaagggttt gtccatgatg tggtctgctg ctgcacgtgc ttcggggctt
     2101 gcgtttgccg gaacaacggc gattcctgcg gcattaagca tgctgacgtc gttgatcgag
     2161 tcgcctgcgg caaggaagtc cgacggggtg aggcccatga gttctgcaag ttttgcaagt
     2221 gctgctcctt tggagagtcc caggggctgg aggtgtatgg caaagccagt gtcgatgacc
     2281 tgaaccggca ttcctgcaag aagtctgctc acgtcttctg cttccacgtc acgggcaaat
     2341 gcgacgtctg agtaccggta ttcgttgctg tagagcctga gttcttctcc tttcggctgg
     2401 aagtgttcca cgatcttttg gtaggcggca aggcagatgc tgcggtctcc ttctgcgtga
     2461 cgcatgccgg tgtagctgtg ccggtagact ccgccgtttt ctgcgatgat gtcgccagag
     2521 gttccgatca ttttggagag tgcgtcaata aaacagacgg tgttgccgga tgccagtatc
     2581 accgggatgc ctgcatccac caggcgacgg atctcttcta cggcgtgcat ggagaggcgc
     2641 cgccggtcat cggtgagtgt tccatctatg tcggtgatga aggctttagg catgaaaaat
     2701 atatttgcgt tggcagggtt tagtgtttgt gcgggaaatg ttatcccgcg gtttttcttc
     2761 ttatattcct cttttgttca ttctgttgct ctgttttatg gctcatcatc attatggggt
     2821 ttgggtatta tgatcagcca aactacaacg ttatgtgggg ttgtgtagta tcttccaaga
     2881 tctccccgaa cggaatgcgg ggagggacgc agagcatgaa caataacaaa ggaatagaga
     2941 gtcttgttgt aataattttc tggaaatggg tcttttgggt tggaaagtgt tttccacttc
     3001 acagttccca ttacacgtac atatggaact cgctgtaagc accaaaaagc gcatataaac
     3061 gggacaccat atgga
//
LOCUS       DALTZZ010000193         2340 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_30375,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000193 DALTZZ010000000
VERSION     DALTZZ010000193.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30375"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            97..570
                     /locus_tag="O0X12_03645"
     CDS             97..570
                     /locus_tag="O0X12_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HJK53281.1"
                     /translation="MAETVEVLVPGGRATAGPPLGPALGPLGINVKAVVDDINKKTAE
                     FNGMSVPVTVTVDDKKNVTLTVGIPPTTALVMKEAGIEKGSGTPNTQAVGNLPLEAVI
                     RIAKMKMESMLSYDLKNAVKEVMGTCVSVGVTIDGKTAKAAIAAVNAGEYDAQLA"
     gene            693..1334
                     /locus_tag="O0X12_03650"
     CDS             693..1334
                     /locus_tag="O0X12_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HJK53282.1"
                     /translation="MVDKTQIISAVTAAMEQAPKRKFQESIDITINLKHVDMAQPKNR
                     IDETILLPQAIGVKKIAVLGKGDIVSQARNAGVDIIIGPEEIERLGGAPREARKLAGQ
                     YDYFLAETAVMPLVGRWLGQRLGPRGKMPQPIPMGQDITPIVERLRNSVKIRSKDRLN
                     MSVKIGNTAMSAEAVAENVDAVLKRVLGRLENGELNIRSVYVKTTMGPAVKVM"
     gene            1337..2182
                     /locus_tag="O0X12_03655"
     CDS             1337..2182
                     /locus_tag="O0X12_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HJK53283.1"
                     /translation="MAIYTHHLPAWKREEVESIVENAGKHELIGLVDVYGIPARQFQQ
                     IRRNLHGNAVVKVARNTLVEHAFNELGGHFLDLNGHVSEHSALIFADGNPFKLFKSLE
                     QTKTKRSAKAGEVAPEDIVVPAGPTSFKPGPIVGELQQAGIPAAIDGGKVKIKETKTV
                     VKAGQAISKKQADVLAKLDIKPMPVGLSLLAVVYEGEIYLPDVLGIDEEAYKAKITLA
                     AQQVFNLAVNAAIPTPCAQITEAQIAKAVREARNLGVEAPIYEKGVIEMIISKAYRQA
                     NALKA"
     gene            2210..>2340
                     /gene="rpl12p"
                     /locus_tag="O0X12_03660"
     CDS             2210..>2340
                     /gene="rpl12p"
                     /locus_tag="O0X12_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866086.1"
                     /note="similar to eukaryotic L12; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HJK53284.1"
                     /translation="MEYIYAALLVHSAGKTVDEESVKAVLSAAGIAVDDSRVKALIAA
                     "
BASE COUNT          637 a          578 c          632 g          493 t
ORIGIN      
        1 ccctatcacc cactaccttt atgttctcat acgtcgacct tattgaacca atgttaggca
       61 gaccttcgaa ggtctgtact gacatacggt gatacaatgg cagaaactgt cgaggtactg
      121 gtacctggcg gtagagcaac tgcaggacca ccacttggtc cagcactggg tccccttggg
      181 atcaacgtga aggcagtcgt tgatgacatc aacaagaaga ctgctgagtt caacggtatg
      241 tctgttccgg taacggttac agtcgatgac aaaaagaacg tcacgttaac agtcggtatt
      301 cccccgacaa ccgcacttgt tatgaaggaa gcgggaatcg agaagggatc cggcactccg
      361 aacactcagg cagttggtaa tctgccgctt gaagctgtca ttcgcattgc aaagatgaag
      421 atggagagca tgctttccta cgacctgaaa aacgcggtca aggaagtcat gggcacctgc
      481 gtttccgttg gtgtgaccat cgacggcaag acggcaaaag ccgcaattgc cgcagtcaat
      541 gcaggcgagt acgacgcaca gcttgcataa agttctgtgt ccggcttcaa acgggtagcg
      601 ggtgaaagaa tcccgcgaaa aatgtacagt agtagagaac aaccatagga gatcggacaa
      661 attccggtcg agaactatgg aggcttcatc aaatggttga caagacccaa attatcagcg
      721 ctgttacggc agcaatggag caggcgccca agaggaagtt ccaggagagt attgatatta
      781 ctatcaatct caagcatgtg gacatggcgc agcccaaaaa ccgtattgat gagacgattc
      841 ttctgccaca ggcaataggc gtcaaaaaga tcgctgtctt gggtaaggga gacattgtgt
      901 cccaggctcg caacgcaggt gtcgatatca ttatcggtcc tgaagagatt gagcgtctgg
      961 gtggtgcccc gcgtgaggca cgtaagctcg caggtcagta tgactacttc cttgcagaga
     1021 ctgcggtcat gccacttgtt ggtcgctggc tcggtcagag actcggtccc cgcggcaaaa
     1081 tgccgcagcc gatcccgatg ggtcaggaca ttactccgat cgttgagcgt cttcggaact
     1141 ccgtcaagat ccggtccaag gacagactga acatgtctgt aaagatcgga aacaccgcca
     1201 tgagcgcgga ggcggttgcc gagaacgtcg atgcggtctt aaagagagta ctgggaagac
     1261 tcgaaaacgg cgaattaaac attcgttccg tctacgtcaa gactacgatg ggtcccgctg
     1321 tgaaggtgat gtaaacatgg caatttatac ccaccacctt cccgcgtgga aacgcgaaga
     1381 agttgaatcg attgtggaga acgccggaaa gcacgagctt atcggcctag tggatgtcta
     1441 cggaattccc gcaagacagt tccagcagat ccgcaggaac ctgcacggca atgcagttgt
     1501 gaaggttgca agaaacacgc tcgttgagca tgcattcaat gagcttggcg gtcacttcct
     1561 tgaccttaac ggccacgtaa gtgaacactc tgcactgatc ttcgccgacg gaaacccgtt
     1621 caagctgttc aagtcccttg agcagacgaa aacaaagcgt tccgcaaaag caggcgaggt
     1681 cgctcctgag gatatcgttg tccccgcagg accaacttcc ttcaagcccg gaccgatcgt
     1741 cggagaactc cagcaggcag gtatcccggc agctattgac ggcggtaagg tcaaaattaa
     1801 agagaccaag accgttgtaa aagcaggcca ggctatcagc aagaaacagg cagacgttct
     1861 ggcaaagctt gatatcaagc cgatgccggt tggtctctca cttctggccg tcgtttacga
     1921 aggcgagatt tatctgccgg atgttcttgg tattgacgaa gaggcataca aggcaaagat
     1981 tactcttgca gcacagcagg tgttcaacct cgcagtcaac gcagcaattc cgaccccatg
     2041 tgctcagatc actgaggctc agattgcaaa ggctgtccgt gaggcacgga accttggtgt
     2101 ggaggcccca atctacgaga agggcgtcat cgagatgatc atcagcaagg catacagaca
     2161 agcaaacgcc ctgaaggcat aattatcata caaaataggt gaagataaaa tggagtacat
     2221 ttacgcagct ctgttagttc actccgcagg aaagactgtg gatgaagaat cggttaaggc
     2281 agttctctct gccgcaggta tcgcagttga cgactcccgt gtaaaggcac ttatcgctgc
//
LOCUS       DALTZZ010000194         2672 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_30581,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000194 DALTZZ010000000
VERSION     DALTZZ010000194.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30581"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /gene="dcd"
                     /locus_tag="O0X12_03665"
     CDS             complement(<1..447)
                     /gene="dcd"
                     /locus_tag="O0X12_03665"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HJK53285.1"
                     /translation="MILVDWEIADHIKRGFIGVDPYDPALVQPNSLDIRLGNHFVWYE
                     PCNDVIDPYKKETILSHTSERKSSYFDIMPGQFVLAETLETITLPDNIVSSIEGKSSV
                     ARLGIELHQTGGWIDAGFSGTITLEMCNVNCRPVRVYAGMPIGQLVF"
     gene            complement(544..1089)
                     /locus_tag="O0X12_03670"
     CDS             complement(544..1089)
                     /locus_tag="O0X12_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HJK53286.1"
                     /translation="MIRLSTPLGAEVLDLHAGDRVLLSGTIYTARDEAHLKIQKDGFP
                     FDPVGAVLYHCGPVVDTKRNVIVAAGPTTSARMNGLTAPMIDAGVRALIGKGGMSAEV
                     REILSRRGVYFAFTGGCAALAASCMGLVACHYPELGMAEAVWEIRVRDMPLVVGIDAH
                     GGDLFADVACEVRERYTKLPL"
     gene            complement(1086..1910)
                     /locus_tag="O0X12_03675"
     CDS             complement(1086..1910)
                     /locus_tag="O0X12_03675"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HJK53287.1"
                     /translation="MDSLLFERLAQAVEDAVREAEIILPPDVEVALRRAANRETNPVA
                     RGEFTNILDNLSKARDLRVPICQDTGIPVIYLTVPPTVPVSCDLYDAVAEGVRRATRS
                     VPLRPNGVDPISRENSGDNTGAGLPPVHIVPGDVLRVTVLPKGAGSENMSQIRMMLPS
                     EVSNIPRFVVDVVMAAGARPCPPIVVGVGIGGTFDGAAALAKEALLEPIDTMDAYEQK
                     ICDVINSLGIGPMGLGGDTTALAVKVKRGFCHTASLPVAVNIQCWCCRRGVREVAL"
     gene            complement(2147..2647)
                     /locus_tag="O0X12_03680"
     CDS             complement(2147..2647)
                     /locus_tag="O0X12_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HJK53288.1"
                     /translation="MVRKPARMYRDLAKKAYCRREYMGGVPGVKVVQFDMGNLSADFP
                     VAIHLEVLEACQIRHTAMEAARINMNRRLMKDVGRNNFHLKIRAYPHHVIREHKQATG
                     AGADRVSEGMRLAFGKAVGTAARVAPRQRIFTVWTTEQYINQAKDALLHSGYKLPTPT
                     RVVVEN"
BASE COUNT          725 a          761 c          609 g          577 t
ORIGIN      
        1 gaagacgagc tgacctatgg gcatccctgc atagacacgg accggacggc agttaacatt
       61 gcacatttca agagtaattg tcccggaaaa tccggcatcg atccagccgc cggtctgatg
      121 gagttcaata ccgagacgtg cgacactgct ttttccttca atggaggata caatgttgtc
      181 ggggagggtg atggtttcaa gggtttctgc aaggacaaac tgtcctggca tgatgtcaaa
      241 ataggaactt tttcgctcgc tcgtgtgaga gagaatggtc tcttttttgt aaggatcaat
      301 cacgtcgtta cagggttcgt accagacaaa gtgatttcca agacggatat ccagtgagtt
      361 cggctgtact agcgcaggat catacgggtc cacaccgata aatccgcgtt tgatgtgatc
      421 cgcgatttcc caatctacca gaatcatatc ttatctatac gaaggcaaca gtaataaacc
      481 ctgtatgaat ttgtgggatt gtcccattga gtttacacag atatttacca gctttgaaaa
      541 atattagaga ggtaattttg tatatcgttc acgaacctca cacgcgacat ccgcaaacag
      601 atcaccgccg tgggcatcaa tgcccaccac cagcggcata tcccgtacac ggatttccca
      661 caccgcctca gccataccca gctcagggta atgacacgca acaagtccca tgcatgatgc
      721 agcaagagca gcacaaccac cggtaaacgc aaagtacaca ccccgtcttg acagtatctc
      781 acgtacttcg gcagacattc cgccttttcc aatcagagcc ctcaccccgg catcgatcat
      841 cggcgcagtc agaccattca tccgtgccga cgtcgtcgga cctgccgcaa cgatcacatt
      901 tcgcttggta tccaccaccg gtccgcagtg atacaacacg gcccccacag gatcaaacgg
      961 aaaaccatcc ttctgaatct tcaaatgggc ctcatcccgt gcggtgtaaa tcgttccgga
     1021 cagaagcacg cgatcccctg catgcagatc caaaacctca gcacccagcg gagtactcag
     1081 acgaatcaca gtgccacctc ccggacccca cgcctgcagc accagcactg gatattcacc
     1141 gcaaccggca gagaggcagt atggcagaat ccgcgcttga cctttaccgc aagtgctgtc
     1201 gtatcacctc caagacccat aggacctatc ccaagtgaat taatcacatc acaaatcttc
     1261 tgctcatatg catccattgt gtcgatcggt tccagaagcg cctcctttgc aagcgcagcc
     1321 gcaccatcaa atgttcctcc gataccaaca ccaaccacta tcggcggaca aggcctcgct
     1381 cctgccgcca tcaccacatc caccacgaac cgcgggatat tcgaaacctc cgacggcagc
     1441 atcatacgaa tctgtgacat gttctctgaa cccgccccct ttggaagcac tgttacacga
     1501 agtacatcac ctggaacaat gtgtaccggt ggaagacccg cgcccgtatt gtccccggag
     1561 ttttcccgag agatgggatc aactccattt ggccggagag gcacagaccg cgtcgcccgc
     1621 cggacacctt cagcaacagc atcatacaga tcacaactta ccgggacagt tggcggcact
     1681 gtcagatata tcacaggaat acccgtatcc tgacagatcg gaacacgcag atccctcgcc
     1741 ttcgaaaggt tatccagaat atttgtaaac tcaccacgcg caacaggatt tgtctcacga
     1801 tttgctgcac gccgaagagc cacctcaaca tcaggaggaa gaataatctc cgcttcgcgc
     1861 accgcatcct ccacggcctg ggccagacgt tcaaacagca gggaatccat gaaaagagta
     1921 ttgcaggaga tatggattaa gactgtcgtt tgaacagacc ccttcacaaa gacgggaaaa
     1981 agaaacatca gggatgttct ttgtttgaaa cagacagaaa gtacattctg cgatggtaaa
     2041 tatcacgaaa aacgccggac gaacaacgag caaatcgaaa aagacacgaa cactaacaaa
     2101 aaactgcaaa aggaaaatat agtattggtt gagagccagg gtaggcttag ttctcaacta
     2161 caacacgggt cggcgtcgga agcttgtatc cgctgtgcag aagagcgtcc tttgcctggt
     2221 taatatactg ctcggtcgtc cagacggtga agatgcgctg tctcggagca acacgtgcag
     2281 cagtacctac agccttaccg aatgcaaggc gcataccctc agaaacacgg tctgcaccgg
     2341 caccggttgc ctgcttgtgc tcacggataa cgtggtgcgg gtatgcccgg atcttcaggt
     2401 ggaagttgtt tcttccaaca tccttcatca gacggcggtt catgttgata cgtgccgctt
     2461 ccattgccgt gtggcggatc tggcatgcct caagaacttc aaggtgaatt gcaaccggga
     2521 aatcagcaga gaggtttccc atatcaaact gtacaacctt aacaccaggc acaccaccca
     2581 tgtattcacg gcggcagtat gccttcttag caaggtcgcg gtacattcgt gctggttttc
     2641 tgaccatttt gaataactcc tcgctctcta aa
//
LOCUS       DALTZZ010000195         2313 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_30604,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000195 DALTZZ010000000
VERSION     DALTZZ010000195.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30604"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="O0X12_03685"
     CDS             <1..1538
                     /locus_tag="O0X12_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HJK53289.1"
                     /translation="STEYFISTLADRWNAGIIMQVLVIGALIALITRMGGMRAVAEAV
                     AKRAKGPRTAQLATWIMGFVIFFDDYANALIVGPIMRPVCDKFRISRERLAYIVDSTA
                     APIAGIFVISTWIGTELVNINEGLAIAGITGVSAYSIFIDTIPYRLYNILALFFVFAS
                     AMLLREYGPMLTAELRARRTGETIRPGSEVEQDDDAVPVEEQEICKEDHAVVATSKKA
                     APANIWNAIIPVGVLIVSSLILFFTNGADAIMAGNGLISAEEFVQLGFIDSVREAYSS
                     SDASIVLFQAALLACIVSIVMGFAQRIFSLKTGIETWAHGMKSMLYVCVILILAWSIG
                     SIIGDLGTAHYLVGALSDSLPIWIVPALIFVIAGVISFATGTAYGTMAILLPLVIPLS
                     AAIAGFSTGTPDASIYSYIVVCSSGVLTGAIFGDHCSPISDTTILSSMGSGCSLIDHV
                     DTQLWYALTIAAVVIIGYIIIGLGINVWITLLITMALLVGVLFVFGKKVPTWKPDEEK
                     LVE"
     gene            complement(1617..>2313)
                     /locus_tag="O0X12_03690"
     CDS             complement(1617..>2313)
                     /locus_tag="O0X12_03690"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HJK53290.1"
                     /translation="LINAIPRHATAVFGVPAKRTAEVEQIFAQFAEEMRERWSSTDPS
                     ISLALLPESPRDEMIPSATKIISALDACPNGVLAMSSTIPDLVQTSTNLSSVRMDGRS
                     LIVATFQRSVSQNEKREASEMVAKTFQSCGAEIRTSSEYPGWMPDPSSPLLQTAITVY
                     TNLFGETPKITATHGGLECGLFRRNYPALDIISIGPNILGAHSPKERLQISSVEKVRL
                     FLAELLKAIGTAA"
BASE COUNT          498 a          577 c          577 g          661 t
ORIGIN      
        1 gttctacgga gtacttcatc tccacccttg cggaccgctg gaatgcaggt attattatgc
       61 aggtactcgt gattggtgca ctgattgctc tcattactcg tatgggaggc atgcgtgcag
      121 ttgccgaggc tgttgcaaag cgggcaaaag gtccgcgtac cgcacaactc gcaacctgga
      181 tcatgggatt tgttatcttc ttcgacgatt acgcaaatgc cctgatcgtc ggtccgatta
      241 tgcgtccggt ctgtgacaag ttccggattt cccgtgaacg tcttgcctac attgtggact
      301 caacagcagc accgattgca ggtatttttg tgatctccac atggatcgga actgaacttg
      361 tgaacatcaa tgaaggtctt gccattgccg gaatcactgg tgtttcagca tacagtatct
      421 tcatcgacac aatcccatac cgcctgtaca acattctggc actcttcttc gtgtttgcct
      481 cagcgatgct tctccgtgag tacggtccga tgcttaccgc cgaacttcgt gcccgccgga
      541 caggcgaaac catccgcccg ggttctgagg tggagcagga tgatgacgct gttcctgtcg
      601 aagagcagga gatctgcaag gaggatcacg ccgttgttgc aacatccaag aaagctgctc
      661 cggcaaacat ctggaatgct atcattccgg ttggtgtgct gatcgtctct tccctgattc
      721 tgttcttcac aaacggtgcc gatgcaatca tggctggaaa cggattaatc tctgctgaag
      781 agtttgtcca gctcggattc attgactcgg tgcgtgaagc ttacagttca tcggatgcaa
      841 gtattgtact gttccaggca gcacttcttg cctgtatcgt ctccattgtg atgggctttg
      901 cccagaggat cttctcctta aagaccggta ttgaaacctg ggcgcacggc atgaagtcga
      961 tgctgtatgt gtgtgttatc ctgattcttg cctggtcgat tggttcgatc attggtgatc
     1021 tgggaactgc acactacctc gttggtgctc tgtccgactc cctgccgatc tggattgttc
     1081 cggctctcat ctttgtcatt gccggggtta tctcgtttgc gacaggaact gcatacggaa
     1141 caatggcaat tcttctgccg cttgttattc cgctttccgc agccatcgcc ggtttctcaa
     1201 caggtacacc ggatgcctct atctactcgt acattgttgt ctgttcatca ggtgtgctga
     1261 ccggtgcaat ctttggagat cactgttcac caatctccga taccaccatt ctctcatcaa
     1321 tgggttcggg ttgtagtctg atcgatcacg tggacacgca gttgtggtac gccttaacga
     1381 ttgcagcagt tgttatcatc ggctacatta tcatcggtct cggcatcaat gtgtggatta
     1441 ctctcctgat tacgatggca cttctcgttg gtgttctatt tgttttcgga aagaaagttc
     1501 cgacctggaa gccagacgag gaaaaactgg tggagtaaat ctccaaaaat atttttttta
     1561 ttttatccgt ttcaggaata attttgtctt tacctttgtt gatgcagaac cgtcctttac
     1621 gccgctgttc cgatcgcttt gaggagttca gcaagaaaca ggcggacctt ttcaaccgat
     1681 gatatctgca gccgttcttt tggggaatgt gcaccgagaa tgttcgggcc aatcgagatg
     1741 atgtcaagtg caggatagtt tctgcggaac agcccgcatt caagtcctcc atgggttgca
     1801 gttatcttgg gtgtttctcc aaagagattt gtatacaccg ttattgccgt ctgcaggagc
     1861 ggagaggaag gatcaggcat ccagccggga tattcactgc tggtacgaat ctccgccccg
     1921 caggattgga acgtctttgc caccatctct gatgcctcgc gtttttcatt ttgactaaca
     1981 ctccgctgaa atgttgctac aatcagggaa cgaccatcca tccgaaccga ggataggttt
     2041 gtagaggtct gtacaagatc cggaattgta ctgctcatgg caagaacacc attcggacat
     2101 gcatcaagag cgctgataat cttcgttgcg gaaggaatca tctcatccct tggagactcc
     2161 ggcagaaggg caagagaaat gctgggatct gtactgctcc acctctccct catctcttct
     2221 gcaaactggg caaatatttg ctcaacctcg gcggtccgtt ttgcgggaac tccaaacact
     2281 gcggttgcgt gacggggaat ggcgtttatc aga
//
LOCUS       DALTZZ010000196         1786 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_30668,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000196 DALTZZ010000000
VERSION     DALTZZ010000196.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30668"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            8..1384
                     /gene="glmM"
                     /locus_tag="O0X12_03695"
     CDS             8..1384
                     /gene="glmM"
                     /locus_tag="O0X12_03695"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HJK53291.1"
                     /translation="MELAKLQKQYFGTNGVRGVTGIDMTPKLALEIAEAFGTMLGPGK
                     TIGIGRDTRTSGPALAAAVRAGLLSCGCNVIDFDIVPTPCLQYLVLDHHLDGGVMITA
                     SHNPPEYNGIKIIEADGTEMGDERTIQLEQTMIHQQFTFAPWNKLGTAATEPNARELY
                     ISAIVAQLPKDCGRGLTVVVDPGNGPAAATTPEILRRLGATVHVINEPFDGTFPGRMP
                     EPTAEGLAPLSKKVVETGAAFGVAHDGDADRAIFVDEHGTFMDGNITLGLVASYFCRK
                     HPGGIVVTPVSTSGVVEAVAESHGCTTTYTVVGSIYAARTMRSLMEEGKPVIIGGEGN
                     GGIIYPAHQFCRDGGMSAATMLSLIAERTMPLSSLIAELPVFTMYQEKRKTGRAAEIT
                     AHMKDYFKDCPVDDRDGIRITRGGAWALIRPSGTEPLVRVYTESKDPAEAKQLMDEIL
                     GEISGYLE"
     gene            1438..>1786
                     /locus_tag="O0X12_03700"
     CDS             1438..>1786
                     /locus_tag="O0X12_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53292.1"
                     /translation="MKIMGFSGKTLRLTAVLALICIAATLSAGCIGVANPTPNQAMVR
                     ENLIFLDPDYVDANFIWNLLLDPHSYDQELINAVDSWLSPTDPVIELGAGVGILSTYI
                     NDKLAIPSQQVSVE"
BASE COUNT          463 a          606 c          411 g          306 t
ORIGIN      
        1 acacgctatg gaacttgcaa aacttcagaa acagtacttc ggcaccaacg gcgtccgcgg
       61 cgtaaccggc atcgacatga cgccgaaact cgccctcgaa atagcagaag ccttcggaac
      121 catgcttggc cctggaaaaa ccatcggcat cggccgtgac acccggacat ccggccccgc
      181 cctcgccgca gccgtccgtg caggcctcct ctcctgcgga tgcaatgtca tcgacttcga
      241 catcgtccca accccctgcc tccagtacct cgtactcgat caccacctcg acggcggcgt
      301 catgatcacc gcatcccaca acccgcccga gtacaacggc atcaaaatca tcgaagcaga
      361 cggaaccgaa atgggagacg aacggactat ccagctggaa cagaccatga ttcaccagca
      421 gttcaccttc gccccctgga acaaactcgg aaccgcagca accgaaccaa acgcccgtga
      481 actctatatt agtgcaatcg tcgcacagct ccccaaagac tgcggccgcg gcctcactgt
      541 cgtcgttgat ccaggaaacg gcccggcagc cgcaacaacg ccggaaatcc tccgtcggct
      601 cggtgcaacc gtccatgtca tcaacgaacc cttcgacgga acattccccg gacgcatgcc
      661 cgaacctacc gccgaaggac ttgcacccct ctccaaaaaa gtcgtcgaaa ccggagccgc
      721 attcggagtc gctcatgacg gagacgccga ccgtgcaatt ttcgttgacg aacacggaac
      781 attcatggac ggcaacatca cactcggcct tgtcgcctcc tacttctgcc gcaaacatcc
      841 gggaggcatc gtagtaacac cggtcagcac ctctggagtt gtagaagcgg ttgcagaaag
      901 ccacggctgc accacaacct acaccgtcgt cggcagcatc tacgccgcaa gaaccatgcg
      961 cagcctcatg gaagaaggaa aacctgtcat catcggcgga gaaggaaacg gcggcatcat
     1021 ctaccctgct caccagttct gccgtgacgg cggcatgagc gctgcaacca tgctcagcct
     1081 catcgccgaa cgaaccatgc cgctttcctc actgatagca gagctccccg tattcaccat
     1141 gtaccaggag aaacggaaga ccggccgagc cgcagagatc accgcccaca tgaaagacta
     1201 cttcaaagac tgtcccgttg acgaccgcga cggcatccgc atcacccgcg gaggagcctg
     1261 ggcactcatc cgcccgtccg gtaccgaacc ccttgtccgt gtctacaccg aatccaaaga
     1321 cccagccgaa gcaaaacagc tgatggatga aatactcgga gaaatctccg gatacctcga
     1381 ataacttttt ttccacaaca acagttacaa atacctcaac atcaagaaac aatactcatg
     1441 aagatcatgg ggttttcggg caaaacgctc cggctgaccg cagtgcttgc cctcatctgc
     1501 attgcagcaa cactctctgc cggatgtatc ggcgttgcca acccgacacc aaaccaggcc
     1561 atggtccggg aaaacctgat atttctggat ccggactacg tggatgcaaa ctttatctgg
     1621 aatctgctcc ttgacccaca cagctatgac caggaactga taaacgccgt ggacagctgg
     1681 ttaagtccca cagacccggt aatcgaactg ggcgcaggcg tcggcatcct ctccacctac
     1741 atcaatgaca aactcgccat cccctcccaa caggtctccg tagaac
//
LOCUS       DALTZZ010000197         3546 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_30689,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000197 DALTZZ010000000
VERSION     DALTZZ010000197.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30689"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="O0X12_03705"
     CDS             complement(<1..803)
                     /locus_tag="O0X12_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HJK53293.1"
                     /translation="MEFPRYDINKYDPKRMMIIPILIFLVAAAIVGFTFINTGMPVTP
                     GIDFAGGTAVTIHTTDTKEQIQAFYAGYPLTSIDEGVGNAGYYIKFGPMSNEEMMKFN
                     EETLAKYPDASIDQIGANFGATLQSQAMVALLFAFIGMAVVVFIAFRKLIPAATVVFA
                     GIADITITAGVMNIIGIELSLATTAALLMLIGYSVDSNILLTSKVLKRQGKLADKMAG
                     AFHTGFIMTTTTLAAIVAMLVVALIGQVPTLYNIAAVLVIGLVCDMIFT"
     gene            complement(877..1695)
                     /locus_tag="O0X12_03710"
     CDS             complement(877..1695)
                     /locus_tag="O0X12_03710"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HJK53294.1"
                     /translation="MKTILYYFTGTGNSLAVARAIAERLPETELVPIPMLLLKGETVR
                     VPKDTNIGIVYPLYAMGLPNIVIRFFGILDLSDAGYVFSVVTEGGKYGSPTKQIAALA
                     KQSGHDLDAAWWIQMPDNYIPLSAPPAKTEQKTIREDALRKVAVLVEAVQERRPRHID
                     LTATGKLLRMVMYKPFMKKIPAFGKKFVVSSNCNGCMVCVDVCPVNNIQVLPKGKKEW
                     LNHCEGCLACLQFCPVEAISCGGKTDERPRYHHPNITAADMKAQKGAAETGRTE"
     gene            complement(2285..3376)
                     /locus_tag="O0X12_03715"
     CDS             complement(2285..3376)
                     /locus_tag="O0X12_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HJK53295.1"
                     /translation="MAHADPNSPYNTMPHLPPEADIETIRIWRACAEAKTSLARMREA
                     GKLIPNQRILISSIPLLEATASSQIENIFTTSNALYTALSVPEERIDPATKEVLRYRE
                     ALSVGVGELKNGKKLGEILLTRVCSVLLDKETDRRGTNAGTYIKGPEGRVYTPPDDPI
                     VLEDLIHHLEDYINGGREPDPLIRLALIHYQFEAIHPFSDANGRTGRILNILYLIDQD
                     LLDIPILYLSRYIIQHKSEYYLRLRRVTENEEWEEWIIFMLNAIQETAEWTYERISAI
                     KCLMDETIEVCKEKLPKIYSYDLVEEIFLQPYCKPAYLVERGIGNRQTAMKYLKALED
                     IGILTSIRIGKERIYINVRLFELLKFDDE"
BASE COUNT          818 a          798 c          901 g         1029 t
ORIGIN      
        1 caggtgaaga tcatatcgca gacaagaccg atgacaagga cggctgcgat gttgtaaagg
       61 gttggaacct gaccgatgag ggcaaccacc agcatggcaa cgattgcggc aagggttgtt
      121 gtggtcatga tgaatccggt gtggaatgca cctgccatct tgtcggcaag ttttccctga
      181 cgtttgagga ctttgctggt caggagaatg ttgctgtcca cagagtaacc gatgagcatg
      241 agaagtgctg cggttgttgc aagtgagagt tcaattccga tgatgttcat gactcctgcg
      301 gtgatggtga tgtcggcgat tcctgcgaag acgacggttg ctgcgggaat cagtttgcgg
      361 aatgcaataa acacgacgac cgccataccg ataaatgcga agagcagggc aaccattgcc
      421 tgtgactgaa gcgttgcacc aaagtttgct cctatctgat cgatacttgc atcagggtat
      481 ttggcgaggg tttcttcatt gaacttcatc atctcttcat tggacatcgg gccgaacttg
      541 atgtagtatc cggcgttacc gactccttcg tcgatggagg tcagggggta ccctgcataa
      601 aatgcctgta tctgttcttt ggtatcggtg gtgtggattg tgaccgcggt acctccggcg
      661 aagtcaatgc cgggggtgac gggcatgccg gtgttgatga acgtgaaccc gacgatggcc
      721 gcggcaacaa ggaagatcag aatcgggatg atcatcatcc gtttcggatc gtatttgttg
      781 atatcatacc ggggaaattc catggtttac ttatattgat tttcaaagga tataataagc
      841 catgcctgtc cggaaatccg gattgaaaaa gaggggttat tcggtgcggc cggtttctgc
      901 tgcgcctttc tgggctttca tgtcggctgc ggtgatgttt gggtggtggt atctggggcg
      961 ttcatcggtt ttgccgccgc aggagatggc ttcaacggga cagaactgca ggcaggcaag
     1021 acagccttcg cagtggttaa gccactcttt tttgcctttg gggagtacct ggatgttgtt
     1081 gacgggacag acatcgacgc agaccatgca cccgttacag tttgagctga cgacgaattt
     1141 tttgccgaac gcggggatct ttttcatgaa cggtttgtac attaccattc tgaggagttt
     1201 tccggttgcg gtgaggtcaa tgtgccgcgg gcgccgttcc tgaactgctt caaccaggac
     1261 tgcaactttt ctgagtgcgt cttcgcggat agttttctgc tcggtttttg cgggcggtgc
     1321 ggataggggg atgtagttgt ccggcatctg gatccaccat gcggcatcga ggtcatgtcc
     1381 gctttgtttg gcgagggctg ctatttgttt ggtgggtgat ccgtattttc ccccttcggt
     1441 gacgacggag aatacgtatc ctgcatccga gagatcaagg ataccaaaga atctgatcac
     1501 aatattcggc agtcccattg catacagcgg ataaacgatg ccgatgttgg tatcttttgg
     1561 tacgcgaaca gtctcccctt tgaggagaag catggggatg gggacgagtt cggtttccgg
     1621 gagccgctcg gcgattgccc gggctacggc gaggctgttg cctgttccgg taaagtagta
     1681 gagtattgtt ttcatggcgg tgatcgaccg gactttttgg tattggtata gagtccggcg
     1741 ttcctcctgt gcatatattt gtcgggcgtg tggttataga ttgtgttaga taaaagggga
     1801 atgtgtttct gttgtatgat gatgtttggg tgtgtctcat gcaggaaaaa aagatcagct
     1861 atcaggaact ggacagcaaa cagcagatca ccatctgcac cctgtctgcc ataccagcca
     1921 aataataatt gatctttctt cttcttttct tcttgtactg tactaataag tacagtacat
     1981 agtagtagta ataggtaata gaaaatgaaa aaaaatcccg gacggtctgc ggtcacatcc
     2041 cctactcttt caggatatcg ctcttatttg ccctgtttag cggatgttgt tgtttatcat
     2101 ctctgttttt tgacttgctc cactacccga attttcctta tccgaatgct gaaattatat
     2161 cagattattt cagaaatctg gaatttgata caccttctct tttatcaaac accctcaaca
     2221 aattcatact ggttgcctgt acgtaacaag caacctctag tttttccaga ccttatcagg
     2281 cgttctactc atcatcaaac ttcagcagtt caaacagcct gacgtttata taaatccgct
     2341 ccttccctat tcgtattgat gtaagaatcc cgatatcctc taaagccttc agatacttca
     2401 ttgctgtctg ccggttccca atcccccgct caaccagata ggctggctta caatatggct
     2461 gtaaaaatat ctcctcaacc aaatcatagg aatagatctt cggcagtttt tctttgcaga
     2521 cttcaatcgt ttcatccatc aggcatttta ttgcagaaat tcgttcatat gtccactctg
     2581 cggtttcctg aatggcattc agcataaaaa tgatccactc ctcccactcc tcattttcag
     2641 taactctccg gaggcgtaag taatactcgg atttatgctg gataatatag cggctgagat
     2701 agagtatcgg aatatcaagc agatcctgat caatcagata gagaatgttc aggattcgtc
     2761 cggttctccc gtttgcatcg ctgaagggat gtatggcctc gaactgataa tgaatcagtg
     2821 ccagacggat cagcgggtct ggttctcttc caccatttat gtagtcttcc agatgatgaa
     2881 tcagatcttc cagaacgatc ggatcgtctg gtggtgtata tacgcgtccc tccggtcctt
     2941 tgatgtaggt cccggcattt gttccccgcc gatccgtctc tttatccagc agaaccgagc
     3001 aaacacgggt aagcaggatt tccccaagct ttttcccgtt cttcaattct ccaactccaa
     3061 ctgacagtgc ctcccggtac cggaggactt cttttgttgc cggatcgatc ctctcttcag
     3121 gaacagagag agcagtataa agggcattgc tggttgtaaa gatattctca atctgggaac
     3181 ttgcggttgc ttccagcagc gggatcgaac tgatgagaat tctctggttg gggatgagtt
     3241 ttcccgcttc ccgcatccgt gcaagcgatg tttttgcctc ggcacaggct ctccatatcc
     3301 ggatagtttc aatgtctgcc tctgggggaa gatgcggcat ggtattgtag gggctgtttg
     3361 gatccgcgtg tgccatggtc tctatgaata ttctatctgt catccatatc tatctgtcga
     3421 aaaaatctaa aaatttcgac acgatgtgtc tgacgtgttc acggagtata caggtgtctt
     3481 attatctgtc gaaaaaatct aaaaatttcg acacgtcacg tatgacatgt tcacggagta
     3541 tacaga
//
LOCUS       DALTZZ010000198         3979 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_30692,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000198 DALTZZ010000000
VERSION     DALTZZ010000198.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30692"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(27..1061)
                     /locus_tag="O0X12_03720"
     CDS             complement(27..1061)
                     /locus_tag="O0X12_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HJK53296.1"
                     /translation="MKATVAEPLRRDGFGEYKLIPESLDDLWHLSHLISWGDTVFAVT
                     LRTVDGPNDKLRAEKLEKRPVRIGVKCEKVEFLPAVGRLRVFGVIVFGPDTGQHHTLN
                     VETGYEISVVRQWRQVDLERLERAVAASVHGVVHIVAVEDGEAEVYRVRQYGPERIAT
                     LTIGSGKTAEIDSRQSLFEELLRALAKVTGPVVVAGPGFVKEDFVKFARSSAPETAER
                     MLLADTRRTGYGAVQEAIGNGVLTRIAEDLQLAREVQVMDEVFLRIGQNGAVAYGPAE
                     VREAIDYGAAETVIVADALVRENRTSKMIEDAERLGAGVVVLSTEFEPGKRLAGLGGV
                     AALLRYKIAT"
     gene            complement(1058..2962)
                     /gene="rqcH"
                     /locus_tag="O0X12_03725"
     CDS             complement(1058..2962)
                     /gene="rqcH"
                     /locus_tag="O0X12_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HJK53297.1"
                     /translation="MATLAGMSGTDVTAMTAELSALLPLWIGKIYQYDNATFGIRLNG
                     EDKARYLLYVVKGVRAHLVSSLPDAPKNPSGFSMYLRKFIDGGKVLAIEQKTVERVLI
                     ISIGKGPREFRLIIELFDEGNLILTDEKYVIQNALVQKRFRERDIVGGAVYSMEGMDP
                     VRLSYEEFAEQMSQDEADVVRALATRMQLGGPVSEEVCALAGVSKSMPAKFATEVQLR
                     PVYEAMVSYLGRLRREMEPVIDEKGAFPLPSVLREPKEHYPTFSAALEAWFPKPVAEA
                     VVEAKVKLSREERIRKQQEEALVKFERKISEAMESSEIIYSHYGEVQETINVLSQASE
                     RMSWQDIAKVLKGSDMPAARRIVSVNPADASVVLDLGEKHRVTIFVHESLEANVGRYY
                     QVAKKFRAKKEGALRAMERAIVHPVKPKSHGPGKMKAKWYHRFRWMETSDGVVVIGGR
                     NADQNEELVKKYMEGKDTFLHADVFGASVVIVKGTAEHVDEAVQFAASYSRLWGSGAA
                     AGDVIAAAPSQVSKTAESGEFVAHGSFVIRGERKIFRNVPMEVAIGVQMEPVVAVLGG
                     TPSAIEPRCQVAVRLRPGTFEGNDVAKKVLRRLKEGLPESDQKLLKAVLNTEAVAAFV
                     PPGGSDLVES"
     gene            3065..3706
                     /locus_tag="O0X12_03730"
     CDS             3065..3706
                     /locus_tag="O0X12_03730"
                     /inference="COORDINATES: protein motif:HMM:NF039857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK53298.1"
                     /translation="MQVARVKKEKCSTADCNRCIRFCPVSRKDQPVIYIGRNKKATVN
                     EELCNGCGKCVRICPEKAIEMITIPDPVDETASTDSGIQSSSPSEVETPKQTLPPKKK
                     ETPEEKTARKKSEHGERVIRTLIACVLGMIAGITSYFIAGTPSAETGVQADPFIGILI
                     LLVAIVLQRTIFILLKIDTAKLGKKDWFYQAFMTFAMWYLSWTIILSTSLLAE"
     gene            3711..>3979
                     /locus_tag="O0X12_03735"
     CDS             3711..>3979
                     /locus_tag="O0X12_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK53299.1"
                     /translation="MRIAIVHKDRCHSRKCGQECIAYCPRVRTGDETIQIGENGRAEI
                     SEELCVGCGICIKKCPFEALDIIQLPEELDTPVHRYGPNAFVLYGL"
BASE COUNT          965 a         1241 c          794 g          979 t
ORIGIN      
        1 aaaaaaattc tttatctcac gtacacctat gttgcgattt tataccgaag cagtgcagca
       61 actccgccca gacctgccag acgctttccc ggctcaaact cagttgaaag cactacgact
      121 cctgctccaa gccgctctgc atcttcaatc atcttggatg ttctgttctc ccgtaccagt
      181 gcatctgcca caatcactgt ctctgccgca ccgtaatcaa tcgcctcccg cacctccgca
      241 ggaccatatg ccaccgcacc attttgtccg atccgcaaaa acacctcatc cattacctgt
      301 acctcccgtg caagctgcag atcctcggca atccgtgtca gaacaccatt cccaatcgct
      361 tcctgtactg ccccatagcc cgttcgccgc gtatccgcaa gtaacatacg ttctgcagtt
      421 tccggtgctg acgaacgtgc aaacttcaca aaatcctcct tcacaaatcc tggacctgca
      481 accaccaccg gtccagtcac cttcgcaagt gctcttagca gttcttcgaa caaactctgc
      541 cttgagtcga tctctgctgt cttcccactg ccaatcgtca gtgtagctat ccgttctggc
      601 ccgtactgtc gaacacgata cacctctgct tccccgtcct ccacggccac aatatgtacc
      661 acaccatgta cagacgctgc tactgcgcgt tccagccgct ccagatccac ctgcctccat
      721 tggcggacta cactgatctc atatcctgtc tccacattca gcgtgtgatg ctgccctgta
      781 tccggtccga atacaatcac gccgaatacc cgaagcctcc caaccgcagg caggaactct
      841 accttctcac actttacccc tatgcgtaca ggtcgcttct ccagtttttc tgcccgaagt
      901 ttgtcatttg gcccgtccac cgttcgcagt gttactgcaa aaacagtatc tccccacgaa
      961 atcagatgtg ataagtgcca cagatcatcc aggctttcgg gtatcagttt atactcgcca
     1021 aacccgtcac gtctcagcgg ctcagctacc gtcgccttca tgactccacc agatcagatc
     1081 cgcccggcgg cacaaatgca gccacggcct cagtattcag gacagctttc agcaactttt
     1141 gatccgactc gggaagtccc tctttcagcc gccgcagtac cttctttgct acatcatttc
     1201 cttcgaaagt ccctggccgc aggcgaaccg ccacttggca ccgtggctcg atcgcagacg
     1261 gtgttccgcc gagaaccgca acaacaggct ccatctggac tcctattgcg acctccatcg
     1321 gtacgttccg gaaaatcttc cgttccccgc gaatcacgaa tgatccgtgc gccacaaact
     1381 cacctgactc tgcagtttta ctcacctggg acggcgctgc tgcaatcaca tcgcctgctg
     1441 cagcacccga tccccataaa cgcgagtatg acgcagcaaa ctgcacagct tcatccacat
     1501 gttctgctgt ccctttcacg atcaccactg atgctccaaa gacatctgcg tgcaggaacg
     1561 tgtcctttcc ctccatgtac ttctttacca gctcctcatt ctgatccgca tttctacctc
     1621 cgataacaac cacaccgtca gatgtctcca tccaccggaa ccgatgatac cattttgcct
     1681 tcatctttcc gggaccatgt gacttcggtt ttactggatg tacaattgcc cgctccattg
     1741 cccgaagtgc tccctccttt tttgcacgga acttttttgc cacctgataa taccgcccta
     1801 catttgcttc aagactttcg tgcacaaaaa tcgtcactcg gtgcttctcg cctaagtcca
     1861 gcacaactga tgcatctgcc ggattcactg atacgatccg ccgtgctgca ggcatatcag
     1921 atccttttag gacttttgca atatcctgcc aagacatccg ttctgaagcc tgagagagta
     1981 cgttgatcgt ctcctgcacc tcgccgtaat gcgaataaat tatctccgag ctctccatcg
     2041 cctcggaaat tttccgctca aactttacga gtgcttcctc ctgctgttta cggatacgtt
     2101 cctcccgaga cagtttcact ttcgcctcaa ccacagcctc cgcaacaggt tttgggaacc
     2161 atgcttcaag agccgctgaa aatgtaggat aatgttcctt tggctctcgc aggacagacg
     2221 gcagcggaaa tgctcctttc tcatcaatca cgggttccat ctcgcgccgc aaccggccaa
     2281 gatacgaaac catggcttcg tagactggtc gcagttgtac ctctgttgca aactttgcag
     2341 gcattgattt tgacacaccg gcaagagcgc agacctcttc tgaaacaggc cctccaagct
     2401 gcatccgcgt tgcaagtgcc cgcaccacat ccgcttcgtc ctgtgacatc tgttctgcaa
     2461 actcctcgta tgaaagacgg acaggatcca ttccctccat tgaatacact gcaccgccca
     2521 caatatcccg ctcacggaac cgcttctgca ccagcgcatt ctggatcaca tacttttcat
     2581 cggtaagaat cagatttccc tcatcaaaca gttcaataat gagccggaac tcccgtggtc
     2641 cctttcctat tgaaatgatc aaaacccgct caactgtctt ctgctcgatc gccagaacct
     2701 ttccgccgtc gataaacttc cgcaggtaca ttgaaaatcc ggatggattt ttcggggcgt
     2761 ctggaaggga actcaccaga tgcgcacgaa ccccttttac cacatacaac aaataacgag
     2821 ccttatcctc accatttaaa cgaattccga atgttgcatt atcatattgg tatattttcc
     2881 ctatccaaag cggcagaaga gcagaaagct ccgccgtcat tgcagtcaca tccgtcccgc
     2941 tcatacccgc aagtgttgcc attgatgata ccaagaatag ataagacctg agaactaaaa
     3001 aaataaagca tctatgccgc agctgtctgc agcggcagac cctcccctcc ggagaatacc
     3061 aaatatgcag gtagcacgag tcaaaaaaga aaaatgcagc actgcagact gcaatcgctg
     3121 catccggttc tgtcccgtct cacgaaaaga tcagccggtt atctacatcg ggagaaacaa
     3181 aaaagcaacg gtcaacgaag agctttgcaa cggatgtggt aaatgtgtcc gcatctgtcc
     3241 tgaaaaagcg atcgagatga ttactattcc tgatcctgtt gacgaaactg cttcaacaga
     3301 ttctggcatc cagtcatcct ccccttcgga agtagaaacc cctaaacaga ctctccctcc
     3361 aaagaaaaag gaaactcctg aagagaaaac tgcccgcaaa aaatcagaac atggcgagcg
     3421 tgtcatacga acgcttatcg cctgtgttct cggaatgatc gcaggtatca cctcctattt
     3481 cattgcggga accccgagtg ctgaaaccgg tgtccaggca gatcctttca ttggtatcct
     3541 gatcctgttg gttgcaattg ttctccagag aacaatcttt atcctcctca agatcgacac
     3601 cgcaaagctc ggtaaaaagg actggttcta tcaggcattc atgacctttg ccatgtggta
     3661 cctttcttgg accatcattc tgagcacatc actcttagcc gagtaatatc atgaggatcg
     3721 ccatcgttca caaagaccgg tgtcactccc ggaaatgcgg gcaggagtgc attgcctact
     3781 gcccgcgggt acggaccgga gatgaaacta ttcagatcgg tgaaaacggt cgtgccgaga
     3841 tctcggaaga gctctgtgta ggttgtggta tctgcatcaa aaaatgtccg tttgaagccc
     3901 ttgatatcat tcagctccct gaagaactgg acacgccggt tcatcgttac ggtccgaacg
     3961 cctttgtcct ctatggtct
//
LOCUS       DALTZZ010000199         4041 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_30859,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000199 DALTZZ010000000
VERSION     DALTZZ010000199.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30859"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="O0X12_03740"
     CDS             <1..1758
                     /locus_tag="O0X12_03740"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HJK53300.1"
                     /translation="VADGYLVDFVSIETKLKFLTEGIIYDDLSTEEKEEYENLFLDEN
                     GDLPKTIDSTALNDWIFNTDTIKEVLAALLKHGIKTDYGSKIGKTIIFAKNHAHAEKI
                     YEIFGKEYPSCPPDYIRVIDNYTNYADSLIDEFKKPNENPRIAISVDMLDTGIDIPEI
                     VNLVLFKKVLSKAKFWQMIGRGTRLCPKLIDGADKEKFYIFDYCANFEFFRINAHGKE
                     PSIVPTVQERIFSLQTEMAYKLQRYEYQTQPLRSFRRELITDLLTKIRELNRDNFAVR
                     QHLRYIDRYRDETAWQALTYEDTLKISEEIAPLIYPYSDEAAAVRFDALMYGLELAWL
                     INKPYPKAKKDLLNKVRAIADISTIPEILAQKELIGKILHTDYIKHADLGDFEHIRKN
                     LRDLMKYLPAGTRTIYETNFSDEILQTEWHISDLTEDYLRTYKEKAAAYLREHQNIPV
                     IEKLRTNIPLNSEDIASLEKILWNDIGSREQYEAECGNKPLGIFVREITGLEMNAAKK
                     AFSTYLQDSRLNPQQIYFLNRIVEYIVKCGIMTDLSVLQATPFTDRGNVVELFSENKE
                     IWYGIRRTIEQINENAKTA"
     gene            1805..2572
                     /gene="larB"
                     /locus_tag="O0X12_03745"
     CDS             1805..2572
                     /gene="larB"
                     /locus_tag="O0X12_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HJK53301.1"
                     /translation="MPDHHTLPEILEHVRDGTLDPAAAEQLISSLPVDEVANLDLNRV
                     ERCGVPEVVLAEGKDTDSLVKIMYSYVRAAGRVLASRISPEQVNAVLADLPEGITADY
                     REICRILILSDGTVPEPSGGTVAIVTAGTSDIRVAEEARAIAEEMGAEVLTAYDVGVA
                     GIHRLFPALEKLRNADIYIVCAGREGTLPSVVAGLVNKPVIGVPVSTGYGYMGQGEAA
                     LASMLQSCAAITVVNIDAGFTAGAVAARTAALIGGKP"
     gene            2569..3075
                     /locus_tag="O0X12_03750"
     CDS             2569..3075
                     /locus_tag="O0X12_03750"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide adenylyltransferase"
                     /protein_id="HJK53302.1"
                     /translation="MKRGLYVGRFQPYHNGHNSVIERIADEVDELIIGIGSAEISHDL
                     RHPFTAGERVLMISRALKNIRIPVYIIPLEDVKRNALWVAHVKSMTPPFDVVYTSNPL
                     VIELFREAGIRVTSPPMYRREPLSGTAIRARMVAGEAWEECVPCEVADVIREIRGIER
                     MQQIAKTD"
     gene            3159..>4041
                     /locus_tag="O0X12_03755"
     CDS             3159..>4041
                     /locus_tag="O0X12_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK53303.1"
                     /translation="MNIIENPLYQKLLLTAVAIGVVMDGLDGSIVNVVLPVIAADFGT
                     DTGTIAWVVITYLLMMAGFLLVFGKLADRGHIRKIFLGGFVIFTAGSAACGLSPDLYC
                     LLGARMFQGIGAAMIAAAAPMLCVKCLPARMLGLALGVLTMASSIGFAAGPALGGILT
                     HYLSWHWIFLINVPIGIAAVPFALRVIPKDGTAEKKPFDLTGAALLFGMMTFGIYALE
                     RLPHLGVSNPQILACTVLCIVFAVLFVFRELRTPTPLINIRVFAAWQFSAVVIAFLII
                     NVVYMGVLYLLPFYLSTG"
BASE COUNT         1126 a         1205 c          898 g          812 t
ORIGIN      
        1 gttgccgacg gctaccttgt tgacttcgta tccatcgaaa caaaactcaa attcctcaca
       61 gaaggaatca tctacgatga tctctcaacc gaagaaaaag aagaatacga aaacctcttc
      121 ctcgacgaaa acggagacct cccaaaaacg atcgacagca ccgcactcaa cgactggatc
      181 ttcaacacag acaccatcaa agaagtactc gcagccctcc tcaaacacgg cataaaaaca
      241 gactacggaa gcaaaattgg aaaaaccatc atcttcgcga aaaaccacgc acacgccgaa
      301 aaaatctatg aaatcttcgg caaagaatac ccaagttgcc ctccggacta catacgtgtc
      361 atcgacaact acacaaacta cgcagacagc ctcatagacg aatttaaaaa accaaacgaa
      421 aatccccgaa tcgcaatctc ggttgacatg cttgacaccg gcatcgacat ccctgaaatc
      481 gtcaacctag tcctcttcaa aaaagtcctc agcaaagcaa aattctggca gatgatcggt
      541 cgcggaacac gcctctgccc aaaactcatc gacggtgcag acaaagaaaa attctacatc
      601 ttcgactact gtgcaaactt cgaattcttc cgcatcaacg cccatggaaa agagccctcc
      661 atagtcccaa cagtacagga acgcatcttc agccttcaga ccgaaatggc ctacaaactc
      721 cagaggtacg aataccagac acaaccactc cgcagcttcc gccgcgaact catcacagac
      781 cttctcacaa aaatcagaga actcaaccgg gacaacttcg ccgtccggca acacctgcgt
      841 tacatcgacc gctacagaga cgaaaccgcc tggcaggccc tcacctacga agacacactc
      901 aaaatctcag aagaaatcgc cccacttatc tacccttaca gcgacgaagc agcggcagtc
      961 cgctttgatg ccctgatgta tggactagaa ctcgcctggc tcataaacaa accttacccc
     1021 aaagcaaaaa aagacctcct gaacaaagtc agggcaatcg ccgacatctc caccataccg
     1081 gaaatccttg cgcaaaaaga actcatcggc aaaatccttc ataccgacta catcaaacac
     1141 gcagacctcg gtgactttga acatatccgg aaaaacctcc gtgacctcat gaaatacctc
     1201 cccgcaggaa cacgaaccat ttacgaaaca aacttcagcg acgaaatact gcaaactgaa
     1261 tggcacatct ctgatctcac tgaagactat ctcagaacat acaaagaaaa agccgccgca
     1321 tatcttagag aacatcaaaa tatcccagtc atcgaaaaac tgcggacaaa cataccccta
     1381 aacagcgaag acattgcatc tctggaaaaa atactctgga acgacatcgg aagccgtgaa
     1441 cagtacgaag cagaatgcgg aaacaaaccg cttggaatct tcgtccggga aatcaccggc
     1501 cttgagatga acgccgcaaa gaaagcattt tccacctact tacaggactc acgcctcaac
     1561 ccgcagcaga tctacttcct caacaggatt gttgaataca tcgtcaaatg cggaataatg
     1621 accgacctct ctgtcctaca ggcaacacca ttcaccgatc ggggaaacgt cgtcgaacta
     1681 ttttctgaaa ataaggaaat ctggtatggc attcgtcgga ccatcgagca gatcaacgaa
     1741 aacgccaaaa ctgcatagcc catcacccta atatcccccg ccgccctact atacaagtac
     1801 aacaatgccg gatcatcaca cactcccgga aatccttgag cacgtgcgag acggcacgct
     1861 tgaccctgcg gctgccgaac aactcatcag cagcctccct gttgacgaag ttgcaaacct
     1921 tgacctcaac cgagtagaac gctgcggtgt acctgaagtc gtcctggccg aaggaaaaga
     1981 cacagactcg ctggtcaaga tcatgtacag ttacgttcgt gctgcgggca gagttttggc
     2041 aagccgcatc agccccgaac aggtaaacgc agtcctcgca gatctcccgg aaggcatcac
     2101 cgcagactac cgtgaaatct gccgtatcct gatcctctca gacggcaccg tcccggaacc
     2161 ctcaggagga accgtggcaa tcgtaaccgc aggaacctcc gacatccgcg ttgcagaaga
     2221 agcccgggcc atcgcagaag agatgggcgc agaagttctg acagcatacg acgtcggcgt
     2281 tgccgggatt caccgcctct tcccggcact tgaaaagctc cggaacgcag atatctacat
     2341 cgtctgtgca ggacgtgaag gaacactgcc ttcggtcgtc gcaggccttg tcaacaaacc
     2401 ggtcatcggc gtcccggtca gtaccggcta cggatacatg ggacagggag aagcagcact
     2461 cgcctccatg ctccagtcat gcgccgcgat aacggtcgtc aacatcgacg caggattcac
     2521 cgcgggggca gtcgcagcac ggacagcagc actcatcgga ggaaaaccat gaaacgcgga
     2581 ctttacgtcg gcagattcca gccgtaccac aacgggcaca attcagtcat cgaacgcatc
     2641 gcagacgaag tggatgaact catcatcggc atcggcagcg cagaaatcag ccatgatctc
     2701 cgccacccat tcaccgcagg agaacgggtg ctcatgatca gccgggccct taaaaatatc
     2761 agaatccccg tctacatcat accattagag gatgtcaaac gaaatgccct ctgggttgcc
     2821 cacgtcaaat ccatgacacc cccgtttgat gtcgtttaca cctcaaaccc gctcgtcatc
     2881 gaactgttcc gcgaagcagg catccgtgtc acctcacctc ccatgtaccg gcgggaacca
     2941 ctctcgggta ccgccatccg tgcccgcatg gtcgcaggag aagcatggga agagtgcgtg
     3001 ccctgcgaag ttgcagacgt tatccgggaa atccgcggca tcgaacgcat gcagcagatt
     3061 gcaaaaaccg actaaccttt tttttcttgg agtactgacg aaaagatata tgatgatgca
     3121 ggcagcacta tatctcaaaa atccggtggt cacctcctat gaatatcatt gaaaacccgc
     3181 tttaccagaa acttctcctc accgccgttg cgatcggtgt cgtcatggac ggccttgacg
     3241 gatccatcgt caatgtggtt ctgccggtca ttgccgcaga cttcggtacc gacaccggaa
     3301 ccatcgcctg ggttgtcatc acctacctgc tgatgatggc agggtttctg ctggtgtttg
     3361 gaaaacttgc tgaccgcggt cacatccgaa agatcttcct cggcggattc gtcatcttca
     3421 ccgcagggtc agctgcctgc ggtctctccc cggatctgta ctgtctcctt ggcgcacgaa
     3481 tgtttcaggg aatcggtgca gccatgatcg ccgcggctgc tcccatgctc tgcgtcaaat
     3541 gtcttccggc aagaatgctc ggtcttgctc tcggagttct cacgatggca agctcgatcg
     3601 gatttgccgc gggtcctgcg ctcggcggga tcctcacgca ttacctctcc tggcactgga
     3661 tctttttgat caacgtcccc atcgggattg cagcagtccc gtttgcactg cgggtaattc
     3721 ccaaggacgg tacggccgag aagaagccgt tcgatctcac gggagcggca ctgctgtttg
     3781 gcatgatgac ctttggcatt tatgcactgg aacggctgcc gcatcttggc gtatccaacc
     3841 cgcagatact ggcatgtact gtcctctgca ttgtgtttgc ggtactgttt gtgttccggg
     3901 agctgagaac accaacaccg ctgattaaca tcagggtatt tgccgcatgg cagttttcag
     3961 cggttgtcat agcgtttctg atcatcaatg tggtgtatat gggagtactg tatcttctgc
     4021 cgttctatct cagtacagga a
//
LOCUS       DALTZZ010000200         7911 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_31349,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000200 DALTZZ010000000
VERSION     DALTZZ010000200.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 7911)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7911)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7911
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31349"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="O0X12_03760"
     CDS             complement(<1..890)
                     /locus_tag="O0X12_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HJK53304.1"
                     /translation="MDSKQKKWLWISIGLSMAILLAVIVLTFNENTIESLKNLNPWFL
                     LLAFCLHMTAMCIWALRIRVMCRSLGYLVPLKHCLNMVCAGQLIASITPSQVGGEPVR
                     IHELYKAKMPVADATAVVLVERLMEAVLLVLGVIIGMSLFSIVYNNNGEVPEMVIAAA
                     WVGTGFFVILLIILMVLLSRPHLIRKIVFKVAGLFTKKWDGERLAKLTIQIDEAIDRL
                     YLTFELFAGRAKQGLVFGFLLTILFWVCEYAIASVIMIGLGYPPNFLLSLVFQLIIAV
                     ILMVPSTPGGAGVAEISYAA"
     gene            complement(948..1988)
                     /locus_tag="O0X12_03765"
     CDS             complement(948..1988)
                     /locus_tag="O0X12_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HJK53305.1"
                     /translation="MNKAQKKWLYISVGISVIALVAMLLLTFDANTIVALEKCNPWYI
                     LLGFLLHILSIVFWALRIKLMCWSLGYHVPFFHSVNLICANMLVAAVTPSQVGGEAVR
                     VYELYKSDVPTADATAVVLMERVFDGIVLGIGTIIGVYLLGKMFSHLDFPAVYMGVAY
                     FATFFFIGALLLFAVLVKRPQWTKIIVAKASGLFTRRWDSQRIERLLGMLDENIDRFY
                     VTIGHFGGRSKVGMALGLLMTVAFWVSEFVIASVLMVGLGLEPMFLISFIFQLLIAMI
                     MMIPLTPGGVGVAEVSIAAFYSIIIPLPLVGVFVLLWRLILYYFNVVVGLIASLRIVR
                     REARVSLTKTQE"
     gene            complement(2109..2501)
                     /locus_tag="O0X12_03770"
     CDS             complement(2109..2501)
                     /locus_tag="O0X12_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0146 family protein"
                     /protein_id="HJK53306.1"
                     /translation="MSVYQIERYVGTYIRDHYSSAVEIGFGGKTVAAEIIQDAGIPIL
                     CTDIHSYQTTVVPAVVDDVFSPTHSYYQGKDVLYAIRPGCEMIPPMIALAEQIGAELI
                     VYHLGFELYENGGEVLDLGDILLHRYVK"
     gene            2567..3172
                     /locus_tag="O0X12_03775"
     CDS             2567..3172
                     /locus_tag="O0X12_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HJK53307.1"
                     /translation="MGVHVFWDSRVPIGLSRPVTEELSGVLEMPVSRIDNGTFPLEGF
                     DPLRQQCDAVKILTKLDIFRRRYPQLFKPDDVDIEFYNKFNHIHEKVLLVTPVDLFEP
                     LADFVFGLAYPALGVAVVSPARLTNEYYGRHPDDDALIDRIVKESAHEIAHLFGLEHC
                     SNSNCIMYCPRNLDDLDRKRKYFCGKCRIQLNGQNEGDLFQ"
     gene            3169..4104
                     /gene="cas1"
                     /locus_tag="O0X12_03780"
     CDS             3169..4104
                     /gene="cas1"
                     /locus_tag="O0X12_03780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HJK53308.1"
                     /translation="MSDAVPWVTVWGYGADIRATRRTLTIREGPVRRSYPLSSIRHLL
                     IAGGHTLETAAVSHLMDNNVPVSFFDIHGEPVGTIRPSGHSAYPLRPAQKSIPVHRFA
                     MSVIITSLKSRMGYLHELGSRRDDGLYYKGELEILTDASHELEYLITLPELSRVFLLT
                     RTMYYEILSRVADPRLGYRRREKPPYLDPINAMLAHGYAVLYASTSVSVAGAGLDPEI
                     GSLYGNVIPPGKNRSACVMDIMEPLMTPMVDRVVMDMAQEGVIAGRYEISSRCLLSEK
                     LMEECNRRFAESINSDDIDRAVSEYAAAITTSTSA"
     gene            4358..5530
                     /locus_tag="O0X12_03785"
     CDS             4358..5530
                     /locus_tag="O0X12_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HJK53309.1"
                     /translation="MLLLALAGKPNCGKSTFFKSLTLANVEIANYPFTTIDANKGVAY
                     VRSPCPCKELGLDNCTSCVDGVRFIPVELLDVAGLVPDAHLGRGLGNQFLDNLREADG
                     IIQVVDASGSTDAEGNPVDIGTRDPREDVEFLRYEFAMWMAGIVDKHLAKLVRQAQGK
                     EAVLIDLLAGALAGLRINAIQIREAVDETGINLAKATPEEIEQMCEVLLRVSKPMIIA
                     ANKADQASEANLASLKELGAVPTIAAGELALKSAAQAKILRYLPGDGTFAPIEGAKLS
                     APQLKALTMIAENMKKFGGTGVQEALNTLVFEEIGMIVVYPVEDDNKCCNAKGVVLPD
                     AFLMPKGSTPKDLAFRVHTDIGNGFLYAVDARTKMRIKDSTELKSGDVIRIVSTAK"
     gene            5573..5890
                     /locus_tag="O0X12_03790"
     CDS             5573..5890
                     /locus_tag="O0X12_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53310.1"
                     /translation="MGSEVYQAQVLRAFFDTITGTDRNLTRIYMCVISLAKLRGEDPE
                     KLRFLVEQMRASKEKRELSIDILDYMAEAANSLEPWAGQSAFGITTPVKSEDFGGISM
                     DSF"
     gene            5983..7452
                     /locus_tag="O0X12_03795"
     CDS             5983..7452
                     /locus_tag="O0X12_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HJK53311.1"
                     /translation="MSVFETVNAVLTSAVAVVNEYIWYLAFIMLGCMGLYFTWKTKGV
                     QLSMLKESCRLSFHGMKASDVLGGKNAVSSFQAFCVSMGARVGVGNISGVGMAFIMGG
                     PGAIFWMWVFALIGAATSFVECTIGQIYKEKGADGLFHGGPAYYIKNALGKGKFAIFV
                     ALLVIITYPICFTQVQANTITDAFVTAFNIDPLIMAVIVTVISAIVILGGIARVARVS
                     SWLVPIMAVVYLIVALIVIAMNFTKIPEMFSVILGCAFGFQQIGGAALGTAIIWGVKR
                     GAFSNEAGIGSIPNVTSAAKVKHPARQGLMQSIGVLIDTLVVCSATAFMVMLLGDYTQ
                     YVAAAGGPDNISGVALVQNMLMQESILGSAAPIIIAVFILVFAFSSMIGYYSMGEANT
                     RFITQNKTVIWVFRIVVIAMIFVACIANFALVWAMADVFMAVMCLVNLGALIFIGKHA
                     FEALADYKKQRAAGIDEPVFDPSILSSQKGISCWPDKDD"
     gene            7535..>7911
                     /locus_tag="O0X12_03800"
     CDS             7535..>7911
                     /locus_tag="O0X12_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJK53312.1"
                     /translation="MECVLLASGSKGNSIYIGNDTDAVVIDAGIGYLKRTLEDLSLDT
                     SRVRALCVTHEHSDHVRSAKAFVKALKIPVYGTGGTLDASVRGGFLPQTAKLIVCRDK
                     LPETCGGLSITTFRTWHDAAEPSG"
BASE COUNT         2020 a         1919 c         1863 g         2109 t
ORIGIN      
        1 aatgcggcat agctgatctc cgccactcct gcaccgccgg gcgttgacgg aaccatcaga
       61 atcactgcaa taatcagttg aaacacaaga gacaacagga agttcggagg gtacccaaga
      121 ccgatcataa tcacggatgc gattgcatac tcgcacaccc agaagagaat tgttaacaga
      181 aatccaaaca caagtccctg ttttgcccgg cctgcaaaca actcaaatgt caggtacagc
      241 cggtcgattg cttcatcaat ctgaatggta agttttgcga gccgttctcc gtcccatttt
      301 tttgtaaata gtcctgcaac cttaaacaca attttccgga taagatgggg ccgtgatagc
      361 agcaccatga gaattatcag tagtatcaca aaaaacccgg taccgaccca ggccgctgca
      421 atcaccatct ccgggacttc gccgttatta ttgtagacga tagagaacaa actcatgcca
      481 atgataacac ctagtacgag cagcacagcc tccatcagtc tttccaccaa gacaactgct
      541 gttgcatccg caacaggcat ctttgctttg tacagctcat ggatacgcac cggttctcca
      601 cccacctgtg acggggtgat tgatgcaatc agctgtccgg cacagaccat attcagacag
      661 tgtttcagcg gaacaagata tccgagcgat ctgcacatta cccggatgcg tagtgcccat
      721 atacacattg ccgtcatgtg cagacaaaat gcaagaagaa ggaaccaggg gttcagattt
      781 ttcaatgatt caatggtgtt ctcgttaaac gttagaacga taactgcaag gagaatcgcc
      841 atcgagagcc cgatagagat ccataaccac tttttctgct ttgaatccat acgggtagtc
      901 aaacctcttt acttactgaa ttgtccgctg gcaatttaaa tctagagtta ttcctgcgtt
      961 ttcgttaagg agacacgggc ttcacgccgc acaatccgca ggcttgcgat aagaccgaca
     1021 accacattga agtagtacag gatcagccgc cacagaagta caaatacacc aaccagcggt
     1081 agcggaataa ttatcgagta gaatgcagcg atactcacct cggcaacacc tactccgcct
     1141 ggtgttaagg gaatcatcat gatcattgcg atcagcagct ggaagatgaa cgagatcaaa
     1201 aacattggtt caagaccaag acccaccatc aggactgaag ctataacgaa ctctgacacc
     1261 cagaaggcaa cggtcatcag cagacccagt gccatcccaa cctttgaacg ccccccaaag
     1321 tgtccgatgg ttacatagaa tctatcgatg ttctcatcaa gcattccaag caatcgttcg
     1381 attctctgtg aatcccagcg gcgggtaaaa agaccagagg cttttgcaac aataatcttt
     1441 gtccactgcg gccgctttac cagaacagca aacagcagga gtgcccctat gaaaaagaac
     1501 gttgcgaagt aggcaacacc catgtatact gctgggaaat caagatgact gaacattttt
     1561 ccaagcagat atacaccgat gatcgtccca atcccaagta caattccgtc aaagaccctc
     1621 tccatcagaa ccactgcggt tgcatcagcg gttggaacgt cggatttgta taattcatac
     1681 acccgtactg cctcaccgcc gacctgtgag ggggtaacag ctgcaaccag catatttgca
     1741 cagatcagat tcactgagtg gaaaaacggc acatgatatc caagcgacca gcacatcagt
     1801 ttgatgcgca gtgcccagaa tacgattgac aaaatatgca aaagaaatcc aagcagaatg
     1861 taccaagggt tgcacttttc taaggcaaca attgtgttgg cgtcaaacgt caaaagaagc
     1921 atagcaacaa gtgcaatcac actgatgccg actgatatat acagccattt cttttgcgcc
     1981 ttattcatgg aaaaaacctg aaaatcatat ggtattggac atttgcaaat aaatagtttt
     2041 tagcttgtct cccgtgtaat ggttttagtg ttgccaaaag aaggtgcggg tatcatgttt
     2101 cgtttggttc atttcacata ccggtgcagc aaaatatctc caagatccag aacttctcct
     2161 ccgttttcat acagctcaaa cccgagatga tacacaatca gttcggctcc gatttgttct
     2221 gcaagtgcta tcatgggagg aatcatctcg catcccggtc ggatagcata cagtacatct
     2281 tttccctgat aataggaatg tgtcggggaa aacacgtcat ctacaacagc aggtacaacg
     2341 gtcgtctgat atgagtgaat gtcagtacac agaatcggaa ttcccgcatc ctgaattatc
     2401 tctgctgcga ctgttttccc cccgaatccg atctcaactg cggatgagta atgatcgcgg
     2461 atgtaggttc ctacgtatct ttcaatctga tatactgaca cacgccttct ccttttgata
     2521 cgtgttcaat atgtttatgt cccgtcacaa tgaaattatc taccacatgg gagtacacgt
     2581 tttttgggac agccgggttc cgattggtct gagcagacct gtcacagagg agctctcagg
     2641 tgtactggag atgcctgtgt cccgtatcga caacggtact tttccgcttg aaggatttga
     2701 tccgctccgc cagcagtgtg acgcggtaaa aattctcacg aaacttgata tttttcgccg
     2761 tcgatatccc cagctcttta agcctgatga tgttgatatt gagttttaca ataaattcaa
     2821 ccacattcat gaaaaagtcc tgctggtaac tccggtagat ctgtttgaac ctcttgccga
     2881 ttttgtattt ggtcttgctt atcctgctct cggagttgct gtggtgtctc cagcccgact
     2941 gacgaatgag tattacggcc gccatcctga tgacgatgca ctaattgatc gtatcgttaa
     3001 ggagagtgcc catgagattg ctcacctctt tggtctggag cactgcagca actctaattg
     3061 tatcatgtac tgtccgcgca atcttgatga tcttgatcga aaacgaaaat atttctgtgg
     3121 caagtgcaga atccaactga acggtcagaa cgagggagat ttgttccaat gagtgatgca
     3181 gttccgtggg ttactgtctg gggatacggt gcagatattc gtgcaactcg gagaaccctc
     3241 actatccgcg agggacctgt gcggagatcc tatcctcttt cctccatccg ccatctgctc
     3301 attgcaggcg gtcatacact tgagacagct gcggtttcac atctgatgga taataatgtc
     3361 cccgtttctt tctttgatat tcatggagaa ccggttggaa caatccgtcc gtctgggcat
     3421 agtgcatatc cccttagacc cgcacagaaa agtatcccgg ttcaccggtt tgcaatgtcc
     3481 gtcattatca cttcactgaa atcgcgtatg gggtatctcc acgaactcgg ttcgcgccgt
     3541 gatgacggcc tgtactataa gggagaactg gaaatactca ctgatgcatc ccatgaatta
     3601 gagtatctta ttactctccc tgaactatcc agagtctttc tgcttactcg taccatgtac
     3661 tatgagattc tctcgcgtgt tgcagatcct aggcttggtt accggcgcag ggaaaaacct
     3721 ccgtatctgg atcccataaa cgcaatgctt gctcacgggt atgctgttct ctatgcaagt
     3781 acttctgtat ctgttgccgg tgctggactc gatccggaga tcggttctct ttacggaaat
     3841 gtcattcccc cggggaaaaa ccgcagtgca tgtgttatgg acatcatgga gcctttgatg
     3901 acaccgatgg tcgatcgtgt tgtcatggat atggcacagg aaggtgtcat cgccgggaga
     3961 tatgagataa gttcgcgctg ccttctttcg gaaaaactga tggaagaatg taaccgccgt
     4021 tttgcagagt ctattaactc agatgatatt gaccgtgcgg tttcagagta tgcggctgcg
     4081 atcacaacca gtaccagtgc ctaagcttcc cggttcttca tgcagtgcga ctgagtttgc
     4141 aggtctttta aacagtcatc agagcggagc caactgcaag acctcctatt cgcaaaccca
     4201 tctgcaatca tcggtggttc ctgatcaaaa tgtgtcggat cattgtcccc aggaattccg
     4261 aaggatacat ctgtctgcat aacctaatca tttcttgaaa atatgcccgg caggaaaaat
     4321 atcgtactcc gccggaaaag ttcaggagat tctactcatg ttgcttcttg cccttgcggg
     4381 aaaaccaaac tgcggaaaat cgacattttt taagtcgctg actcttgcaa acgttgagat
     4441 tgccaactac ccgttcacca ccattgacgc aaacaaaggt gtcgcctatg tgcggtcccc
     4501 ctgcccctgt aaagaacttg gtctggacaa ctgcacctcc tgtgttgacg gtgttcgatt
     4561 catccctgtt gaactcctgg atgtagcagg tcttgttccc gatgcacacc ttggcagagg
     4621 tcttggcaat cagttccttg ataatctccg ggaggccgac ggtattatcc aggttgttga
     4681 tgcatcggga agtactgatg cagaaggtaa cccggttgat attggtaccc gcgatccccg
     4741 tgaagatgtg gagttcctca gatacgagtt tgctatgtgg atggcgggga ttgttgacaa
     4801 acatcttgcc aaacttgttc gtcaggcaca gggcaaagag gctgtgttaa tcgatcttct
     4861 ggcaggagca cttgccggtt tacgaatcaa cgcaatccaa atccgtgaag cggtggatga
     4921 aaccggcatc aaccttgcaa aagcaacacc tgaggagata gaacagatgt gcgaagttct
     4981 gcttcgtgtt tcaaaaccca tgatcatcgc tgcaaacaaa gcggatcagg catccgaggc
     5041 aaaccttgca tcactcaaag aacttggtgc tgtccccact attgcagcag gtgagcttgc
     5101 ccttaagtct gcagcacagg caaagatcct ccgttatctc ccgggagatg gtacgtttgc
     5161 gccgatagaa ggtgcaaaac tttcggcacc gcaactaaaa gcgcttacca tgattgcgga
     5221 gaacatgaaa aagttcggtg gaaccggtgt gcaggaggca ctcaacactc ttgtctttga
     5281 ggagatcggt atgattgttg tctatcccgt tgaggatgac aataaatgct gcaatgcaaa
     5341 aggtgttgtt ctgcctgatg ccttcctcat gccaaaaggc agtaccccca aagatcttgc
     5401 attcagggtg catactgata tcggaaacgg attcctctat gctgtcgacg cccgtacaaa
     5461 aatgagaatc aaagacagta ccgagctgaa atccggtgac gttattcgga tcgtcagtac
     5521 cgcaaaatga cggcaatcta tattacctat cagccgcaaa gttagagtaa ttatgggaag
     5581 cgaggtgtat caggcacagg tccttcgggc attctttgat accattaccg gtaccgacag
     5641 gaatctgacc cgtatctaca tgtgtgtgat atcccttgct aaactccggg gtgaagaccc
     5701 tgaaaagctc agatttttag ttgagcagat gcgggcaagc aaagaaaagc gcgaactctc
     5761 catcgatatt cttgattata tggcagaagc agccaactct cttgagcctt gggcggggca
     5821 gtccgcattc ggcatcacaa cccctgtgaa gtccgaggat ttcggcggaa tctcaatgga
     5881 ttccttctaa atagttccat acgttaggaa cttcttttca ttttttgttg tggcgatacg
     5941 ttacaacacg ttaatatgtt acgatgagaa aagatttatc tcatgagtgt gtttgagact
     6001 gtcaacgcag tactcacctc agcagtagcg gtggtgaacg agtacatctg gtacctcgca
     6061 ttcatcatgc tcggttgtat ggggttgtac ttcacttgga aaacaaaggg tgtgcagctt
     6121 agtatgctca aagagtcctg ccgtctttcc ttccatggaa tgaaggcaag tgatgttctt
     6181 ggtggtaaaa atgctgtatc ttcttttcag gcgttttgtg tcagcatggg cgcccgtgtt
     6241 ggtgtaggta acatttctgg tgtgggaatg gccttcatca tgggtggacc tggtgctatc
     6301 ttctggatgt gggtctttgc tctgattggc gcagcaacga gttttgttga gtgtacaatt
     6361 ggtcaaatct acaaagagaa aggtgccgat ggtctgttcc acggtggacc tgcctattac
     6421 atcaagaatg cccttggtaa aggtaagttt gctatctttg ttgcacttct tgtcattatc
     6481 acgtatccga tctgctttac gcaggtgcag gcaaacacca tcactgatgc gtttgtcaca
     6541 gcgttcaaca ttgatcctct gattatggca gttattgtga cagtgatctc tgcaatcgtc
     6601 attcttggtg gaattgctcg tgttgcacgg gtatcctcat ggcttgtccc aattatggcg
     6661 gttgtgtacc tgatcgttgc actgattgtc attgcaatga acttcaccaa gattccggaa
     6721 atgttctccg tcatccttgg ttgtgcattt ggcttccagc agattggtgg tgcagccctt
     6781 ggtacggcca ttatctgggg tgttaagcgc ggtgcattct ctaacgaagc tggtattggt
     6841 tccatcccca atgtcacctc tgctgccaaa gtcaaacacc ctgcacggca gggtctgatg
     6901 cagtcgattg gtgttcttat cgatacgctt gtcgtctgtt ctgcaaccgc cttcatggtc
     6961 atgcttcttg gcgactacac ccagtatgtt gcagctgccg gtggaccgga caacatcagt
     7021 ggtgtggcac tagtacagaa tatgctgatg caggaatcaa tcttaggttc agcagcaccg
     7081 atcattatcg cagtcttcat tctggtcttt gcattcagca gtatgatcgg ttattactcg
     7141 atgggtgaag ccaacacgcg tttcatcaca caaaataaga ctgtcatctg ggtcttccgg
     7201 atagtggtta ttgctatgat cttcgttgcc tgtattgcca acttcgcgct ggtctgggca
     7261 atggccgatg tcttcatggc tgttatgtgt ctggtgaacc ttggagctct catcttcatt
     7321 ggcaaacacg cctttgaggc tctggctgac tacaagaaac agcgtgcagc aggcattgat
     7381 gaaccggtgt tcgatccgtc tatcttatcc tcccagaaag gtatctcctg ctggccggac
     7441 aaggacgact aatctccctt tgggagacca tccttttttc gactgccaag tcgggtgaat
     7501 atatcttgca gcactcccta cctactacgt gaatatggag tgcgttctgc ttgcaagtgg
     7561 aagcaaagga aacagcatct acatcgggaa tgatactgat gcggtggtaa ttgatgcagg
     7621 aatcggttat ctcaagcgta cgctggagga tttgtctctt gatacctcac gcgttcgtgc
     7681 attgtgtgtt acacacgagc attcggatca cgttcgctcc gcaaaggcgt ttgttaaggc
     7741 actgaaaatt ccggtatatg gcactggtgg tacgctggat gcctccgttc gtggaggttt
     7801 cctcccgcag actgcaaaac tgattgtctg ccgagataaa cttccggaaa cctgcggcgg
     7861 cctttcgatc accacatttc gtacatggca tgacgctgcc gaaccctccg g
//
LOCUS       DALTZZ010000201         2932 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_31468,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000201 DALTZZ010000000
VERSION     DALTZZ010000201.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31468"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            143..1237
                     /locus_tag="O0X12_03805"
     CDS             143..1237
                     /locus_tag="O0X12_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJK53313.1"
                     /translation="MNILFAVCGEGLGHASRSTKLAAYLEQHGHSCSFASYGKAYDFI
                     QKQGFSVHEISREVTLGGDEGLFSLAKTLWLSKSVPASLLNSYRDTRKLLEGQRVDIL
                     VADTMYAAGYAAKSLGIRNAFITNQNTFASAAAPDAVYWNGLGKMVQTYLRKVPDVVI
                     VPDFPPPHTISGYNLEIPQSETDHYRFVGPIFDRDPSAVALEKETIFASFGGEPFKLP
                     LYAMLREIADEMPDLRFEVFSTTPGLPESTENFQTFGYVPDLQPYLAKSRAAILHGGL
                     TSLMEALSFGKPVVMIIDPYHPEQWNNARKIEEMSAGIMIRGDAVTREKLRDAIRQAV
                     SLTPPDLREMFSSVNGRESVRTILEELHTA"
     gene            complement(1285..2673)
                     /locus_tag="O0X12_03810"
     CDS             complement(1285..2673)
                     /locus_tag="O0X12_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HJK53314.1"
                     /translation="MSEKKCESSFKKLILIGVTVGIISGFGAYLFFKGLEYGTAFVME
                     YLFGAYLPLEGQTATEVAAWTPPPVIWLILPIICAGALLSGLIVYFFAPEAEGHGTDA
                     AIRAYHGDGKIRWRVPIVKAVASIITISTGGSAGREGPTAQIAAGFGAFIADIFHLSP
                     RERKIAIATGIGAGIGTIFKAPLGGAILAAEILYLRDFEADVVMPSFLASIIGYSIFG
                     FFEGYAPIFGTTSIVWSVAQIPLFLILGALAAGIGIFYVKTFYGTKDLFDRFAVRYHI
                     PKYCKPVLGAFLIGVLIIVLSYLSPETMIVGMASLGSGYGFVQMAMYNMLPLSVLILL
                     PFVKTITTSLTIGSGGSGGVFAPGLAVGGFAGGAFGMFLHLILPDLVPLATVPAFVIV
                     GMLALFGGIAHAPIAIMIMVLEMTGDFSLFVPAMGAVAVAYLLIGKQTIFHEQRLCRE
                     LPEEYWDGPHTK"
     gene            complement(2743..>2932)
                     /locus_tag="O0X12_03815"
     CDS             complement(2743..>2932)
                     /locus_tag="O0X12_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53315.1"
                     /translation="CYSILFLQLGIPIEALGIAVVLEILLDFLSTALNMLAVPIDMVQ
                     VAAKLDMLDEEKLKSSSA"
BASE COUNT          740 a          733 c          821 g          638 t
ORIGIN      
        1 cctccgcact tctttttttt tggatatgaa ccggaacatc acgttcccac ccgaaaagat
       61 atctgttcac aaatgaaatg agatttcatc gaaaaccggg ggacgaatac ataaccattt
      121 acatctactg ccggagcgtc cgatgaacat cctgtttgcc gtctgcgggg aaggactcgg
      181 tcatgcctcg cggtcaacaa agcttgcagc atatcttgaa cagcatggcc actcctgttc
      241 gtttgcctct tacggaaaag cctacgattt tattcagaag caggggttct ctgttcacga
      301 gatcagccgt gaggttacac tcggcggaga tgaaggactc ttcagccttg caaaaaccct
      361 ctggctctcc aaaagtgttc ctgcatctct tctgaactct taccgtgata cccggaagct
      421 gcttgagggg cagagggtgg atattcttgt tgccgacacg atgtatgccg caggctatgc
      481 ggcaaaatcc cttgggatca gaaacgcatt cattaccaac cagaacacct ttgcctctgc
      541 cgcagcaccg gatgccgtgt actggaacgg gctggggaag atggtgcaga catatctccg
      601 gaaagtcccc gatgttgtca tcgttccgga ctttccccct ccgcacacga tcagcggcta
      661 caatctggag atcccgcaat cggaaacgga tcattaccgg ttcgtcgggc cgatctttga
      721 tcgggatccg tccgcggttg cactggagaa ggagaccatc tttgcaagct tcggcggaga
      781 gccattcaaa cttccgctgt atgcaatgct gcgcgagatt gcggatgaga tgccggatct
      841 gcggtttgag gtcttctcca ccacgccggg tttgccggaa agcaccgaaa actttcagac
      901 attcggctat gttccagatc ttcagccgta tcttgcaaag tcgcgggcag caattctgca
      961 cggcggcctg acatcgctga tggaggcact ctcgtttgga aagccggtgg tcatgatcat
     1021 cgacccgtat catccggagc agtggaacaa tgcccgaaag atcgaggaga tgagtgccgg
     1081 gatcatgatt cggggggatg cagttaccag agaaaagctg agagatgcga tccggcaggc
     1141 ggtttcactg acgccgccgg atctgcggga gatgttttcc tctgtaaacg gcagggagtc
     1201 tgtccgcacc attctggaag agttacatac tgcataggat ctaggcaaaa aagagaaaaa
     1261 atttctccag gggtactgtg ccagctattt tgtgtgcgga ccgtcccagt actcctccgg
     1321 cagttcacgg cagagacgct gttcatggaa gattgtctgt ttacctatca gcagataggc
     1381 aacggcaacc gctcccattg ccgggacaaa cagcgagaag tcaccggtca tctcaaggac
     1441 catgatcatg attgcgatcg gggcgtgggc aatgccgccg aacagagcaa gcattccaac
     1501 gattacaaac gccggaacag ttgcaagcgg tacgaggtcc ggcaggatga gatggagaaa
     1561 catgccgaac gctccccctg caaagcctcc gactgcaagt cccggagcaa acactccgcc
     1621 gcttccgccg gatccgatcg taagagatgt ggtaatggtt tttacaaacg gaagaagaat
     1681 gagaaccgac agcggcaaca tgttgtacat cgccatctgc acgaaaccat acccgctgcc
     1741 gaggcttgcc ataccgacga tcattgtctc aggcgagagg taggagagga cgatgatgag
     1801 aaccccgata agaaatgcac cgagaaccgg tttgcagtac ttcggaatgt gataccggac
     1861 ggcgaagcgg tcgaagaggt cttttgttcc gtagaatgtt ttcacataga agataccgat
     1921 tcccgcagca agggctccga ggatgaggaa gagggggatc tgtgctaccg accagacaat
     1981 ggatgtggtg ccgaagatcg gggcgtatcc ttcaaaaaac ccgaagatgg agtagccgat
     2041 gatggatgca agaaacgagg gcatgacaac atccgcttca aaatcacgca gatacaaaat
     2101 ctcggcggca aggattgcac ctccaagcgg agctttgaag atggtaccga taccggcacc
     2161 gataccggtt gcaatggcaa ttttccgttc acgcggggag agatggaaga tgtctgcaat
     2221 gaatgcacca aagccagccg ctatctgggc ggtcgggcct tcccgtccgg cacttccgcc
     2281 ggttgagatg gtgatgatgg atgcaactgc tttgacgatc gggacccgcc accggatttt
     2341 tccgtcaccg tgatatgcac ggattgctgc atcagtaccg tgaccctcgg cttcgggggc
     2401 gaagaagtag acgatgagac cggagagaag ggcacctgcg cagatgatgg gcagaatcag
     2461 ccagataacc ggcggcggtg tccatgcggc aacttctgtt gcggtctgtc cttcaagggg
     2521 aagatatgca ccaaagaggt attccataac aaaagcggta ccgtattcga ggcctttgaa
     2581 gaagagatat gcaccgaatc ctgagatgat gccgacggtt acgccgatga ggatcagttt
     2641 tttgaaagaa ctttcacact ttttttcgct cacgtctcaa catccccgta tgtaatctga
     2701 tactgtccgc tgaatctata aaaaatgagc gggattgagg tttcaggcag aactgctttt
     2761 gagtttttct tcgtcgagca tgtcgagttt tgcggcgacc tgaaccatgt cgatcgggac
     2821 agcgagcatg ttgagggcag tgctgaggaa gtcaagcaag atttcaagga caactgcgat
     2881 tccgagggct tcgattggga tgccaagctg gaggaagagg atggagtaac ag
//
LOCUS       DALTZZ010000202         3850 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_32099,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000202 DALTZZ010000000
VERSION     DALTZZ010000202.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3850)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3850)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3850
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32099"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            4..417
                     /gene="rpsK"
                     /locus_tag="O0X12_03820"
     CDS             4..417
                     /gene="rpsK"
                     /locus_tag="O0X12_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HJK53316.1"
                     /translation="MSGTEGDLIRISFMAIGTTTKLLSTLSAGDCLRDVVGPLGMPSD
                     IAKGPETVVIVGGGVGVACTPIIAQAAKEKGITGLHIRVRAPGNGKQRSPGPGAQAAI
                     RALSRAGMRIGRIEDVTPVPHDSIRPAGGRRGRRV"
     gene            418..1257
                     /locus_tag="O0X12_03825"
     CDS             418..1257
                     /locus_tag="O0X12_03825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HJK53317.1"
                     /translation="MDLVFSRLDDSVARFTISGATPAFANALRRSMIGEVPTLAIEDV
                     RIYDNTSVLFDEILAHRLGLIPIKTDLSQFVPRSKCTCGGAGCPQCTVTFTLSVEGPG
                     MVTSGDLISMDPRTVPVHNNIPIVKLWEGQKIVLEATAELNTGLEHAKWQPTLACGYK
                     EFPVITIHDSCDACGKCVSECPRDVLEVADNMVHVRVVNGESKEKDCSMCRLCETACM
                     NTGIGENPAITIGADSTKFIFVVESDGSLPVKEIIERALLHIKSRSTDLTDALQDIST
                     EGL"
     gene            1258..1611
                     /locus_tag="O0X12_03830"
     CDS             1258..1611
                     /locus_tag="O0X12_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HJK53318.1"
                     /translation="MNKTNPRLESLIGMLKRASRENEANIWREIAGRLNTSSKNYAEV
                     NIGKISRYAKENEIILVPGKVLAAGVIAAPVKVAALNFSDAAREKIMEANGTCMTIEE
                     LVAANPKGSRVRILR"
     gene            1618..2040
                     /locus_tag="O0X12_03835"
     CDS             1618..2040
                     /locus_tag="O0X12_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HJK53319.1"
                     /translation="MVTVIDGENLLLGRMCSLVAQRVLAGEEIAIINAEKVIVSGSRA
                     MIMKEYYTKRVRGSVEGGPFYPRRPDQIVKRTVRGMIPYKTRPGAAAFRRVKAYVGVP
                     YEFKDAEAEVLEAAHRDRLSSARYVTVGAISTNLGAKY"
     gene            2054..2452
                     /locus_tag="O0X12_03840"
     CDS             2054..2452
                     /locus_tag="O0X12_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HJK53320.1"
                     /translation="MKIINTSGKRKTAIARATLKEGKGRVRINSVPLEVYGSEIIRMK
                     ISEAIALAPGALDNVDVDIDVSGGGVMGQAEAVRTALGRGILNWTNDPHIKEVYLSYD
                     RTLLVNDSRQKEAKKPHGRGARARFQKSYR"
     gene            2545..2733
                     /locus_tag="O0X12_03845"
     CDS             2545..2733
                     /locus_tag="O0X12_03845"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HJK53321.1"
                     /translation="MIPVRCFTCGKVISTVWEEYQQRKAAGEDPKEILDSLGLERYCC
                     RRMLLSHKETVDELYPYT"
     gene            2748..2822
                     /locus_tag="O0X12_03850"
     tRNA            2748..2822
                     /locus_tag="O0X12_03850"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2784..2786,aa:Pro,seq:cgg)
     gene            2860..3048
                     /locus_tag="O0X12_03855"
     CDS             2860..3048
                     /locus_tag="O0X12_03855"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HJK53322.1"
                     /translation="MIYTRYERARIIGARALQISMGAPILVRTTKIDPLEIALAEFAE
                     NMVPITVKRPIGDKVEVQ"
     gene            3050..>3850
                     /locus_tag="O0X12_03860"
     CDS             3050..>3850
                     /locus_tag="O0X12_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HJK53323.1"
                     /translation="MTTIDSITLRRILDSRGNETIEAEILTASGGYGRACAPSGASTG
                     IYEAKVRPCAEAIADAQARLIPELIDLDAQDQRTFDETLRNVDGTEDLSGIGANIAVA
                     LSLANAKAAASALNLELFQYLGGAFVDQTPLPLGNVIGGGAHAVDATDIQEFLIVPTG
                     AATAAEAVFTNARVHKTIKEILVARGKGCGKGDEGAWAPHISDAEAFEIVAEAVNKVQ
                     DATGIEVRMGLDVASSEMWNADLERYVYKNAKRTTEEQIAYIAELVDQY"
BASE COUNT          964 a          997 c         1057 g          832 t
ORIGIN      
        1 acgatctccg gaaccgaggg cgatctgatc agaatttcgt ttatggcaat cggcacaaca
       61 acgaaacttc tctcaacact ctccgcaggt gactgtctgc gggacgtggt cggacctctc
      121 ggaatgccaa gcgacatcgc caaaggcccg gaaactgtag tgattgtggg aggcggtgtc
      181 ggtgttgcct gtacgcctat cattgcccag gcagcaaagg aaaagggaat caccggactt
      241 cacattcgtg tccgtgcacc aggaaacggc aagcagcgct ctccgggacc gggagcacag
      301 gccgcaatcc gtgcgctttc ccgtgcaggt atgcgtatcg gcagaatcga agatgtaacc
      361 ccggtaccgc acgacagcat ccgcccagcg ggtggtagaa gaggcaggag agtctgaatg
      421 gatcttgtat tcagcaggct cgatgactct gtcgcacggt ttactatcag cggagcgact
      481 ccggcatttg caaacgccct cagacgctct atgatcggcg aggtgccaac ccttgccatc
      541 gaagacgttc gcatctatga caacaccagc gttctctttg acgagatcct tgcacacaga
      601 cttggactta ttccgattaa aaccgacctg tcccagtttg ttccgcgtag caaatgtacc
      661 tgtggtggtg caggctgtcc gcagtgcacg gttacgttca ctctgagtgt tgaaggtccg
      721 ggcatggtca cctccggtga tctgatctcc atggacccgc gcacagtccc tgtgcacaac
      781 aacattccaa ttgtgaagct ctgggaaggt cagaagattg ttctggaagc caccgccgaa
      841 ctcaacaccg gtcttgagca tgccaaatgg caaccgactc ttgcctgcgg ttacaaagag
      901 ttcccggtaa tcacgatcca cgacagctgt gatgcctgtg ggaaatgtgt ttccgaatgt
      961 ccgcgcgacg ttcttgaagt ggccgacaac atggttcacg tccgcgtagt aaacggcgaa
     1021 agcaaagaaa aagactgttc aatgtgccgt ctctgtgaga ccgcatgtat gaacaccgga
     1081 attggtgaaa accccgcaat caccatcggt gccgacagta cgaagttcat cttcgttgtc
     1141 gaaagcgacg ggtcccttcc ggtcaaagag atcatcgaaa gagcactgct gcatatcaag
     1201 tcacgatcca cagacctgac tgatgcatta caggacatat ccacggaggg actgtaaatg
     1261 aataagacaa acccccgcct cgaatcctta atcggtatgc tcaagcgggc atcccgggaa
     1321 aacgaggcta atatctggcg tgagattgca ggacgcctga acacgtccag caagaactac
     1381 gctgaagtta acatcggaaa gattagccgc tacgcaaaag agaatgagat catcctggtt
     1441 ccgggtaaag tcctggcagc aggtgtgatc gctgcacccg tcaaggttgc cgcacttaac
     1501 ttctccgatg ctgcgcgtga aaaaatcatg gaagccaacg gtacctgcat gacaatcgag
     1561 gagcttgtcg cagcaaaccc gaagggcagc cgcgtccgga tcctgaggtg aactgaaatg
     1621 gtaacagtta ttgatggaga gaatcttctg ctcggaagaa tgtgcagcct tgttgcccag
     1681 cgtgttcttg caggcgaaga gatcgctatt atcaacgccg agaaagtcat cgtctccggt
     1741 tcccgtgcga tgatcatgaa agagtactat accaagcgtg ttcgtggttc cgttgaaggc
     1801 ggtccgttct acccgagacg ccctgaccag atcgtcaagc gtaccgtccg cggcatgatc
     1861 ccgtacaaga cccggccggg agcagcagct ttccgccgtg tcaaggcata tgttggtgtt
     1921 ccttacgagt ttaaggacgc agaggcagag gttcttgagg ccgcacaccg cgaccgctta
     1981 agcagcgcac gttacgtcac cgttggcgca atcagcacca accttggagc aaagtactaa
     2041 gaggtggaga aagatgaaga tcattaacac cagtggtaag agaaagacgg caattgcacg
     2101 cgcaaccctc aaagagggta agggcagagt ccgtatcaac tctgtcccgc tcgaggtcta
     2161 cggaagcgaa atcatccgca tgaagatctc cgaggcaatc gctctcgcac cgggtgcact
     2221 cgacaacgtt gatgttgaca tcgatgtctc cggcggaggc gtcatgggcc aggcagaggc
     2281 tgtccgcacg gcactcggcc gcggcatcct gaactggacc aacgacccgc acatcaaaga
     2341 ggtttacctc agctacgacc gtacgcttct tgtcaatgac tcccgtcaga aggaagccaa
     2401 gaagccgcat ggacgcggtg cacgtgcacg gttccagaag tcgtaccgtt aaatctagct
     2461 ttcagaatat gggttctgca aaggggatga tatcctcacc cccatttgca aaacaacaga
     2521 ttaataacct tagaaggata actaatgata ccagttcgat gtttcacctg tgggaaagtg
     2581 atttccacgg tatgggaaga atatcaacag cggaaagctg ccggtgaaga tccgaaagaa
     2641 attctcgact ctctcgggct tgagcggtat tgttgcagac gcatgcttct ctcgcacaaa
     2701 gagactgttg atgagctgta tccctacacg tgatgcatga atgctgcggg gtcgtagggt
     2761 agcctggacc atcctattac gttcgggacg tagtgacctg agttcgaatc tcagcgaccc
     2821 catttttcca ttctttatgt tttatggatg tgattgccaa tgatatacac tcgttatgaa
     2881 cgggccagga tcatcggtgc gcgcgcttta cagatctcga tgggtgcccc tattcttgtc
     2941 agaactacta agatcgatcc gcttgagatc gcccttgccg agtttgcaga gaatatggtt
     3001 cctatcaccg ttaagcgccc cattggtgac aaagtagagg ttcagtaata tgaccacaat
     3061 tgacagcatt acgctccgga gaatccttga cagccgtgga aatgagacca ttgaagcgga
     3121 gatcctaact gcaagcggag gatacggccg tgcatgcgca ccaagcggtg catcgaccgg
     3181 tatctatgaa gcaaaagtgc ggccatgtgc tgaagcaatt gctgatgcac aggcacggct
     3241 gattccggaa ctgattgatc ttgatgcgca ggatcagcgt acctttgatg agacgctgcg
     3301 caatgtggat ggtaccgagg atctctccgg cattggtgca aacattgcag ttgcactctc
     3361 tcttgcaaac gcaaaggcag ctgcatctgc cctgaatctt gagctgttcc agtaccttgg
     3421 cggagcattt gtggatcaga ccccgcttcc gctcggcaat gtgatcggcg gcggtgccca
     3481 tgcggtggac gcaaccgata ttcaggagtt cctgatcgta ccgactggtg ctgcaaccgc
     3541 cgcagaggca gtctttacaa acgcccgtgt gcacaagacc atcaaagaga ttcttgttgc
     3601 gcgcggtaaa ggctgcggca agggtgacga gggtgcctgg gctccgcaca tcagcgatgc
     3661 agaagcattc gagatcgttg cggaagcggt aaacaaagtg caggatgcga caggaattga
     3721 ggtccgcatg ggtcttgacg ttgcatcttc cgagatgtgg aatgctgatc ttgagcggta
     3781 tgtgtataag aatgcaaagc gcactactga ggagcagatc gcatacatcg ccgagctggt
     3841 ggatcagtac
//
LOCUS       DALTZZ010000203         2145 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_32711,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000203 DALTZZ010000000
VERSION     DALTZZ010000203.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32711"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="O0X12_03865"
     CDS             complement(<1..1252)
                     /locus_tag="O0X12_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HJK53324.1"
                     /translation="MTEIHIVGTAHVSQKSIDEVREAVDTINPDVIAIELDPGRFAAL
                     KQQMKEAKDAADGTAPTPDNHESPEIRDLLKGNFTLMLVQWILAYVQRKIGMNVGIEP
                     GAEMKEAIRLAEERGIRLVLIDRDIRITLARFWGGMKILEKIKLIWALMQSIFAADDE
                     DDETAEGIDKVAIDELTNPDIIEMALEEFHKFSPNGAKALIDERDAYLAHGVIDLEKS
                     LYERAVVVVGAGHVPGITKYRENPSTLPPMAELLAKPKHYPWGKIIGGLFVAMFAVII
                     IAIAFSGATDLLVWAIIYWVLLHALFAGVATLLVRGHPFSAAVAAALAWATSLNPFLA
                     AGWFAAIVEAKMRPPTAGDLKAISKAESLGEMFSIPLFRILVVAAVANVGSSLATVCF
                     FVFLTPLLGVDLDMMTQILMTGLSN"
     gene            complement(1249..1797)
                     /locus_tag="O0X12_03870"
     CDS             complement(1249..1797)
                     /locus_tag="O0X12_03870"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HJK53325.1"
                     /translation="MHETERVKQQVLEAGFSCLLCGACCSGPDNEVMVSPPEIEMLAE
                     ATGMSCDEIAEPYPEWMQQDGCTFTFGWVLRRGEDGNCIFLENNRCRVYQNRPHICRT
                     YPFMLDGEELIVSECPGCSKERATEDAGQITKDLLRRRDAEDRELEKTEKQYQKHSIS
                     TGSTIVFDSNGAHEYSPTRKKP"
     gene            complement(1801..>2145)
                     /locus_tag="O0X12_03875"
     CDS             complement(1801..>2145)
                     /locus_tag="O0X12_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00303 family protein"
                     /protein_id="HJK53326.1"
                     /translation="GYSGKLFLAGGTQMLAAAAVIRALGNTVPDVVTTSYVYNDSSAT
                     FRETAAAIGCDAYYVDPEFDTLGHAGLGRYAEGEIKEGSGAGGAMFLASLLGYTPEQI
                     KDAIKNHIAAYD"
BASE COUNT          451 a          607 c          548 g          539 t
ORIGIN      
        1 gatttgacag gccggtcatg agaatctgtg tcatcatgtc cagatccacg ccgagcagcg
       61 gtgtcaggaa cacaaagaaa cagaccgtcg caagcgaact tccaacattt gccaccgccg
      121 caacgaccaa tattctaaac agcggaatgg aaaacatctc gccaagtgat tctgcttttg
      181 agatagcttt cagatcgcct gcggttggcg gccgcatctt tgcttcaaca attgcggcaa
      241 accatccggc tgcaaggaac ggattcagtg aagttgccca ggcaagcgct gcggcaactg
      301 ccgcggaaaa gggatgtccg cgaaccagaa gtgttgcaac cccggcaaag agtgcatgca
      361 gaagaaccca gtaaatgatt gcccatacca gcagatcggt tgccccggaa aatgcgatgg
      421 caatgatgat caccgcaaac atggcaacga acagcccgcc gatgatcttt ccccaggggt
      481 aatgtttcgg ctttgccaga agctctgcca ttggcggaag ggttgacggg ttctcccgat
      541 atttggtaat tcccgggacg tgtcctgcgc cgaccaccac cacggcacgc tcgtacagac
      601 tcttctccag atcgatcact ccgtgggcga gatatgcgtc acgctcatca atcagtgctt
      661 tcgctccgtt tggcgagaac ttatggaact cttcgagcgc catctcgata atgtccggat
      721 tggtcagttc atcaatggct accttgtcga taccctccgc cgtctcgtcg tcctcatcgt
      781 ctgcggcaaa tatagactgc atcagtgccc agatgagttt gattttttcc agaatcttca
      841 tcccgcccca gaacctggca agcgtaatcc ggatatcacg gtcgatgaga acgagccgga
      901 tgccgcgctc ttcggcaagt ctgattgcct ctttcatctc ggcgcccggt tcaatcccga
      961 cgttcatccc gatcttgcgc tggacatagg ccagaatcca ctgcacaagc atgagggtga
     1021 agttgccttt cagcaggtcc cgtatctcag gagactcatg gttatctggt gtgggggctg
     1081 ttccgtctgc cgcatctttg gcctctttca tctgctgttt cagtgcggca aagcgtccgg
     1141 gatccagctc gatggcaatg acatcggggt tgatagtgtc tactgcttca cggacttcgt
     1201 caatgctttt ctgcgaaacg tgggcagttc cgacaatatg tatttcagtc atggtttttt
     1261 tcgggtgggg gagtactcat gtgccccgtt gctgtcgaac acaatggttg atcctgtact
     1321 tatactatgt ttttgatact gtttctcggt tttttccagt tcgcggtcct ctgcatcgcg
     1381 tcttcgcaga agatcttttg tgatctgacc ggcatcctct gttgcccttt ctttactgca
     1441 tccggggcac tcggagacga tgagttcctc cccatcaagc atgaacggat atgtccggca
     1501 tatatggggg cgattctggt aaacacggca ccggttgttt tcaagaaaga tacagttccc
     1561 gtcctcccct ctccggagaa cccagccgaa tgtgaaggta catccgtcct gctgcatcca
     1621 ctcgggatac ggctcggcta tttcatcaca gctcattccg gttgcctctg caagcatctc
     1681 tatctccggc ggtgagacca tgacctcatt gtcaggtccg ctgcagcacg ccccgcagag
     1741 cagacaggaa aacccggcct cgagcacctg ctgtttgact ctttccgttt cgtgcatcgc
     1801 ttagtcgtag gcggcaatgt ggtttttaat tgcgtccttg atctgctccg gcgtgtaacc
     1861 gagaagactt gcaagaaaca tcgctcctcc tgctcccgat ccctctttga tctcgccttc
     1921 cgcgtatctt ccgagtcctg catggccgag tgtgtcaaac tcgggatcca catagtatgc
     1981 gtcgcatcct atggccgctg ctgtttcccg gaatgtggcc gaggaatcat tatagacata
     2041 ggaggtggta acaacgtcag ggactgtgtt tcccagagcg cgtatgaccg ctgctgccgc
     2101 aagcatctgc gttcccccgg caaggaagag cttgccggag taacc
//
LOCUS       DALTZZ010000204         2032 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_32783,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000204 DALTZZ010000000
VERSION     DALTZZ010000204.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32783"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            2..517
                     /locus_tag="O0X12_03880"
     CDS             2..517
                     /locus_tag="O0X12_03880"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HJK53327.1"
                     /translation="MNTAMEKIAAEGTPYQNINFGSFMFEVAAAEGLVQDRDQMRKLD
                     RTVQKRLQKLAAEKIAAIDGNVIIDTHASVKTPNGYLAGLPEWVITAIMPDVLVLVET
                     DNDQILVRRLSDETRVRDMEGAKSIAEHQEMNRAFAAAYAMLTGCTVKIIVNADFLLD
                     KAVEELTATLR"
     gene            524..1153
                     /locus_tag="O0X12_03885"
     CDS             524..1153
                     /locus_tag="O0X12_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HJK53328.1"
                     /translation="MEFGKKYGMYIALAVVFGMMLLYGWPEARTFISGIVDIVIGPFA
                     ALGLPFFALVLILATLTGLYSSLIQKYSIDYEKMRENQEKFKEFNVLFREAQRSGDEK
                     AIKKMQARQQAMMAEQMEMSQQQFKPMAYILVLTVPIFFWLIEHIPQNVDLTMAHADL
                     SNAIILPFAGLSSYFDIYLGFFPLWILWYMLCSITVSQVIRKALNIGGM"
     gene            1157..1693
                     /locus_tag="O0X12_03890"
     CDS             1157..1693
                     /locus_tag="O0X12_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJK53329.1"
                     /translation="MRITISGSPGSGTTSLGKALAEKYNLRYLSAGEVFRGLAKEHGM
                     DLAAFGKLAEENPEIDLKIDARQKEIGEASDDIILEGRLAGWMVENADLKILLYASPD
                     CRAERIAERENLTPEEACSATIEREACEAARYMEYYEIDIADPSPYDLVINSETFNQE
                     EVVAIAESVMAIISAKKN"
     gene            complement(1724..>2032)
                     /locus_tag="O0X12_03895"
     CDS             complement(1724..>2032)
                     /locus_tag="O0X12_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53330.1"
                     /translation="LLKLAPECDTLICKGSENAYRSILPPVANHYAKDADDFAMRLNR
                     LTDEEFVALVDLIREGKEGISCIPEECVGVLIEQTLRRVGQDAGNELYALYEAGAQCL
                     "
BASE COUNT          507 a          511 c          499 g          515 t
ORIGIN      
        1 aattaacacc gcaatggaaa agatcgccgc agaaggtact ccctaccaga atatcaattt
       61 cggcagtttc atgtttgaag tcgctgctgc ggaaggtctt gtccaggatc gtgaccagat
      121 gagaaaactt gaccgtacgg ttcagaagcg cctgcaaaag ctcgctgcag aaaagatcgc
      181 cgcaattgat ggtaacgtaa tcatcgatac ccatgcctcc gtcaagacgc cgaacggata
      241 tcttgccggt cttccggaat gggtcatcac tgcaatcatg ccggatgttc tcgttcttgt
      301 tgagacggac aacgatcaga tcctcgtccg cagactgtcc gacgagactc gtgtacgtga
      361 catggaaggt gcaaagtcca ttgcagagca ccaggaaatg aaccgtgcgt ttgctgcagc
      421 ctatgcgatg ctgactgggt gtacggtgaa aatcatcgtt aatgctgact tcctccttga
      481 caaagctgtt gaggaactta ctgcaaccct gaggtaacct ttcatggaat ttggcaaaaa
      541 gtacggaatg tacatcgccc tcgccgtagt gttcggcatg atgcttctct acggctggcc
      601 ggaagcacgt actttcatct ccggcattgt tgatattgta attggtccct ttgccgctct
      661 tggtcttccg ttcttcgcac ttgtcctgat ccttgcaaca cttacaggtt tgtactcctc
      721 gctgattcag aagtactcca tcgactatga aaagatgcgt gagaaccagg aaaaattcaa
      781 ggagttcaac gttctgttcc gtgaagcaca gcgttccggc gatgagaaag ccatcaagaa
      841 gatgcaggcg cgccagcagg caatgatggc tgaacagatg gagatgagcc agcagcagtt
      901 caaacccatg gcttacatcc tcgttctgac ggtgccgatc ttcttctggc tgatcgaaca
      961 catcccgcag aatgttgatc tgaccatggc acacgcagat ctgagtaacg ccattatcct
     1021 tccgtttgca ggactttcca gttactttga tatctacctt ggattcttcc cgctttggat
     1081 tctctggtat atgctctgtt ccattacggt cagccaggtc atcagaaaag ccctcaatat
     1141 tggaggcatg taatcgatgc ggatcacgat cagcggatcc ccgggatcag ggactacctc
     1201 tcttgggaaa gcccttgccg agaaatacaa tctccgctat ctctccgccg gtgaggtctt
     1261 ccgtggtctc gctaaagaac acgggatgga tcttgcagca tttggaaagc ttgctgaaga
     1321 aaaccctgag attgatctca agattgatgc ccggcagaaa gagattgggg aagccagtga
     1381 cgacattatc ctggaagggc gtcttgccgg atggatggtg gaaaatgctg atctaaagat
     1441 cctgctgtat gcatctccgg actgccgtgc cgagcggatc gctgagcgtg aaaatctgac
     1501 tcctgaggag gcatgttccg caaccattga gcgtgaagcc tgtgaagcag cacggtacat
     1561 ggagtactac gagattgaca tcgctgatcc ctccccctat gatctcgtaa ttaactctga
     1621 gacgtttaat caggaagagg tcgttgccat tgccgagtca gtgatggcaa ttatttctgc
     1681 aaagaaaaac tgagttgttc agagaccttt tctccaatta tttttacaga cactgggctc
     1741 ctgcctcata gagggcataa agttcgtttc ctgcatcctg ccccacacgg cgaagtgtct
     1801 gctcgattaa aacaccaaca cactcttccg ggatgcaact aattccctct tttccttccc
     1861 ggatcagatc aacaagagca acaaactcct cgtctgtcaa acgattcagc cgcattgcga
     1921 aatcgtcagc atcctttgca taatggtttg caaccggtgg gagaattgaa cggtaggcgt
     1981 tctctgatcc tttacagatc agggtatcac actcgggtgc cagttttagg ag
//
LOCUS       DALTZZ010000205         3310 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_32807,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000205 DALTZZ010000000
VERSION     DALTZZ010000205.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32807"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            198..1904
                     /locus_tag="O0X12_03900"
     CDS             198..1904
                     /locus_tag="O0X12_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HJK53331.1"
                     /translation="MTETQGSPKIGGNIGNYDETYRTFSLVTPKYYNFAFDVVDAWAK
                     KDRNHLAMIWVNQQGVEKKFTFWDMMIHSNEAANILMKFGIQKGDRVLLMLPRVPEWW
                     ILVLGIMKLGAVFCPSPHMLTVKDIAYRIKVGNFKMVITDSDNMDKVDEVAADCPNLQ
                     LRMLVDDKPGERLPTPWIGYQNELLYPAPVSTKLVSSVGRKMLSTDPMLIYFTSGTTK
                     DPKMVLHDYGHPLGQTVTAKFWHDLTEYDVHFTVSDTGWAKCGWGKIYGQWICGACIF
                     VYDYRTKFHATELLPLIERYGITSFCAPPTIYRMLIIADLKKFSFSELRTCTSAGEPL
                     NPEVIRIWKEGTGLTIREGYGQTETCCCIATMPGMEVKQGSMGKPVPGWHIQLHDDDG
                     NEVPQGETGRIAISLDPRPAGLIREYLDNPEENAAMFVNGWYYTGDKAYKDEDGYFWF
                     VGRNDDVIKSSGYRISPFEVESTLLEHPAVKESAVVGSPDSIRGMVIKAFIVLHDGYQ
                     PSEKLVKDIQDYVKRTTAPYKYPRQIEFVTELPKTISGKIKRAELRNRELQRFAEENG
                     EN"
     gene            2011..2901
                     /locus_tag="O0X12_03905"
     CDS             2011..2901
                     /locus_tag="O0X12_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HJK53332.1"
                     /translation="MTTLQEYLKNSGVYEDLSKIISLIADQAGPIRAAFISNQNYADS
                     KNASGEQQAAMDTWADSHITRVLAKSGLVRAVASEEQDDITTFSSSAKYSVVMDPLDG
                     SSLISVNLCVGTIVGIYDGDVLQPGKNLKAAFYILYGPMTTLTLSVGHGVAIFAQDSD
                     GVYQLLSDNVKMPEGNLYGSGGLRTEWLPEHAKFISDVEGAGGKNRYSGSFVADFHQV
                     LKYGGVYAYPPTTKSPDGKLRLVFEVNPIGFLAVQAGGAVSNGVSSTLTVVPKKVHQR
                     TPIYVGSRGMIEAIEKIHSA"
     gene            2905..>3310
                     /locus_tag="O0X12_03910"
     CDS             2905..>3310
                     /locus_tag="O0X12_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HJK53333.1"
                     /translation="MATTISVCALPLGGIAGSAAMFPDVLQKAAKVLKEAEGSLLTDS
                     LVTHVADWLVLVMVHGQGGASPLITALKDAAVAAARDTADKRRLFCRDIQPVMCGMTF
                     VERESEPFVLFLSSAASPAFWNKTLLSQFADPF"
BASE COUNT          872 a          791 c          854 g          793 t
ORIGIN      
        1 aatctcatct gcgattacgc cggacgcaac gatattttcg atcttcagcg aatcgtatgt
       61 tttcccatcc atctctattt aatacgagag actcaagcat aatacttcgt gtccggtgaa
      121 tcatcccaaa cggagcttga ctttcatatc cactcggatt aattacctca gacatcccat
      181 taatattgac aagaaccatg acagagacgc aaggttcccc aaaaatcggg ggcaacattg
      241 gcaactatga cgagacgtac cgtacgttct cccttgttac gccaaagtat tacaacttcg
      301 cgtttgatgt agtcgatgcc tgggccaaaa aggatcgcaa ccacttggcg atgatctggg
      361 tcaaccagca gggtgttgaa aagaagttca ccttctggga catgatgatt cactcaaacg
      421 aggccgcaaa tattctgatg aagttcggaa ttcagaaagg agacagagtt ctcctgatgc
      481 ttccgcgcgt tccggaatgg tggattttgg ttctcggcat tatgaagctg ggtgcagtgt
      541 tttgtccctc gccccatatg ctgacggtga aggatatcgc ctatcgtatc aaggtaggca
      601 acttcaagat ggttatcacc gacagcgata acatggacaa ggtcgatgag gtcgcagctg
      661 actgtccaaa cctccagctc cgtatgttgg tggacgataa gccgggcgaa cgattgccaa
      721 ccccatggat cggctaccag aatgaactgc tgtatcctgc tccggtctca acgaaactcg
      781 tcagcagtgt cggccgcaaa atgctttcga ccgatccgat gctgatttac ttcacctccg
      841 gaaccaccaa agatccaaag atggtgctgc acgactatgg ccaccccctg ggtcagacgg
      901 ttacagcaaa gttctggcat gatctaacgg aatatgatgt acactttacg gtatcggata
      961 ccggatgggc aaaatgcggc tggggtaaga tctacggtca gtggatctgc ggagcgtgta
     1021 ttttcgtcta tgattatcgt acgaagttcc atgcaaccga gcttcttcca ctgattgaac
     1081 gttacggaat tacatcgttt tgtgcaccgc cgacaatcta ccgtatgttg atcatcgcag
     1141 atctgaagaa gttctcgttt agcgagctgc ggacctgtac cagtgcagga gaaccattga
     1201 atcctgaagt aatccgtatc tggaaggaag gtaccggcct tacaattcgc gaaggatacg
     1261 gccagaccga gacctgctgc tgtatcgcaa cgatgccagg catggaggtc aaacagggtt
     1321 caatgggaaa accggttccc ggttggcaca tccagcttca tgacgatgac ggtaacgaag
     1381 tcccgcaggg agagaccgga aggattgcta tctcattgga tccaagacca gctggtctta
     1441 tccgcgaata cctcgataac cctgaggaga acgcagcaat gtttgtcaac ggctggtact
     1501 acaccggtga caaggcgtac aaggacgagg atggatactt ctggtttgtc ggaagaaacg
     1561 atgatgtgat caaaagttcc ggctaccgga ttagtccatt tgaagtggag tctacacttc
     1621 tggaacaccc cgccgtcaaa gagagtgctg tggttggaag ccccgattcc atccgcggta
     1681 tggtgattaa agcattcatc gtcctgcacg atggatatca gccatccgag aaactggtga
     1741 aggatattca ggattatgtg aagcgcacta ccgcaccata caagtatccg cggcagattg
     1801 agtttgttac agaactgccg aaaaccatct ctggaaagat taagcgcgcc gaactgcgaa
     1861 accgtgaact tcagcggttt gctgaagaaa acggtgagaa ctaatttttc ttttttcaag
     1921 cagtaagtga cactgcatcg catgcctcgg taaaacttgc gatgaatccc ttatctatcg
     1981 tcacgtccaa tatatattca gtgaatacgc atgacgactc tacaggaata tctcaaaaac
     2041 tcgggcgtat atgaagatct cagtaaaata atttcactga tagctgatca ggcaggccca
     2101 atacgtgcgg cgtttatcag taatcagaat tatgctgact caaaaaatgc ctccggagag
     2161 cagcaggccg caatggatac gtgggcagac tcacatatca cccgtgttct cgcaaaaagc
     2221 ggcctggtac gtgcggtcgc atcagaagag caggacgaca tcactacatt ctcatcatcg
     2281 gcaaagtact cggtggtgat ggatccgctg gatggctcct cccttatctc agtgaacctc
     2341 tgtgttggta caattgtagg catttacgac ggggatgtac ttcaacctgg aaagaatctg
     2401 aaagccgcat tctatattct ctacgggcca atgacgacgc ttacactttc ggttggtcat
     2461 ggagttgcaa tctttgctca ggacagtgac ggcgtgtatc agctgctttc agacaatgtc
     2521 aagatgccgg aaggaaacct ctatggatcc ggtggtctga gaacagagtg gctgccggaa
     2581 catgcaaagt tcatctctga tgtagaagga gcaggcggca aaaaccggta ctcgggctca
     2641 ttcgtcgcgg acttccacca ggtgttaaag tatggaggag tctatgcgta tcctccaacg
     2701 acaaagtctc cggatggaaa actgagactg gtgtttgagg tcaatccaat cggatttttg
     2761 gcagtccagg caggaggagc agtttcaaac ggagtttcga gtacacttac cgttgtcccg
     2821 aaaaaggtac accagagaac accgatctat gtcggtagcc gcggcatgat tgaggcaatc
     2881 gaaaaaattc acagtgcata acgtatggca acaacaattt ccgtgtgtgc gctgcctctc
     2941 ggcggcattg caggatctgc agcaatgttc ccggacgtgt tacagaaagc ggcaaaggtg
     3001 ttgaaagaag cagagggatc gcttctgacg gattccctgg tgacacatgt tgctgactgg
     3061 ctggtgctgg taatggttca tggtcaggga ggagcatccc ctctcatcac ggcgctgaag
     3121 gatgcagccg ttgcagcggc acgtgatacc gcagacaaac ggcgcttgtt ctgccgcgat
     3181 atccaaccag taatgtgcgg catgacgttt gtagagcggg agagcgagcc gttcgtgttg
     3241 tttctctcaa gtgcagcaag tccagcgttc tggaacaaaa ccctgctttc ccagtttgct
     3301 gatccgtttg
//
LOCUS       DALTZZ010000206         3967 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_33357,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000206 DALTZZ010000000
VERSION     DALTZZ010000206.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33357"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="O0X12_03915"
     CDS             complement(<1..700)
                     /locus_tag="O0X12_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HJK53334.1"
                     /translation="MGHRISTQARGKGGSTYRAPSHQYKAELKHFGSFTETVTATVVD
                     IEHDPARHTPIAVVKIDGKKEYVLITEGVGVGKEIAWGPEAKIENGNTLPLALIPTGM
                     AVCNIEARPGDGGKFVRASGVQAVVIGKSEGKVGVRMPSGKPKWFHEHCLATIGLVAG
                     GGRGDKPVLKAGKQYHKMKTSATRWPRVRGVAMNVIDHPFGGGGHQHPGKPKTVARGT
                     PAGKKVGHVAARRTG"
     gene            complement(712..960)
                     /locus_tag="O0X12_03920"
     CDS             complement(712..960)
                     /locus_tag="O0X12_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HJK53335.1"
                     /translation="MALAHPLVTEKAMVLLENSNQLSFIVRKEATKESIKAALEKNFG
                     KKVASINTMMTSKGTKKAIITFEENNAAEEILSQLGIV"
     gene            complement(963..1709)
                     /gene="rpl4p"
                     /locus_tag="O0X12_03925"
     CDS             complement(963..1709)
                     /gene="rpl4p"
                     /locus_tag="O0X12_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HJK53336.1"
                     /translation="MKANVKAINGSVLHEIELPAVFNEEFRPDLIKRAVLAYQSEQYQ
                     PYGANPYAGMRTSAEGWGSKRGESKIPRIKNGSRGAKVPQTKGGHPAHAPKVEKILVE
                     KINKKEKAKAIRSAIAATVNTELVTARGHKFEGDAAIIVEDAFETLAKTAEVKKALIA
                     LGVGADLERAKNSRAIRAGRGKTRGRKYKQAKSVLIVTAAPGFTAGANLAGVDVMPVT
                     SLNANVLAPGTHAGRLTVWTEAAVKKLGEA"
     gene            complement(1718..2719)
                     /locus_tag="O0X12_03930"
     CDS             complement(1718..2719)
                     /locus_tag="O0X12_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HJK53337.1"
                     /translation="MRSVRPRAGSLAYYPRKRAKSIVPKYQSWPEYNGQPTLQGFAGY
                     KAGMTHVIMIDDHKKSPTEGKEIMVPVTVIEIPAMKVAAIRVYVKDTYGKHPLTEVWS
                     EDVAALSGRITKAKAHNAAAATEKINAAMADVVEVMVLMYTQPETLTGVPKKVPDLME
                     IRVAGGSAQERFDYALSLLGTSVEMSSILSEGQFADITGITKGKGFQGAVKRFGITLR
                     KRKHARTKKTRHIGTLGPWTPHHVRWQVPMPGQMGFQQRTEFNKRIIKLGDNPDEINP
                     AGGFLHYGLVRGNYVLIKGSIPGPAKRLIRIRPATRMGEQTIQTPVVEFVSLQSKQG"
     gene            complement(3343..3747)
                     /locus_tag="O0X12_03935"
     CDS             complement(3343..3747)
                     /locus_tag="O0X12_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfx family DNA-binding protein"
                     /protein_id="HJK53338.1"
                     /translation="MKDTLLTDRQKEVIRYRKQGMTQQQIADRLGTSKANICTIEKSA
                     NENIRRAKETLEFLYTLDAADLCVLAAGTDLMEAPHLIYQAAAPLNIKIRYDSISLIN
                     RISSYMPEKIKGRHVREDIRVYLNTDGDLYFG"
BASE COUNT          847 a          988 c         1031 g         1101 t
ORIGIN      
        1 atccggttct ccgtgctgca acatgtccga ctttcttacc ggccggagtt ccacgtgcaa
       61 cggtctttgg cttacctggg tgctggtgtc ctccaccacc gaacgggtgg tcgataacgt
      121 tcatagcaac accacggaca cgcggccaac gggttgccga ggtcttcatc ttgtgatact
      181 gtttacctgc tttcagaacc ggcttgtcac cgcgtcctcc accggccaca agaccaatgg
      241 ttgcaagaca gtgctcgtgg aaccactttg gtttgccgga cggcatgcgg acaccgacct
      301 tgccctcgga tttaccgatg acaactgcct gaacaccgct tgcacggaca aactttccac
      361 cgtcaccagg gcgtgcctca atgttgcaga cagccatacc ggtcgggatt aatgcgagcg
      421 gaagagtgtt tccgttctcg atctttgcct caggtcccca ggcgatttct ttgccgacgc
      481 caacaccttc ggtgataagc acgtactctt tcttgccgtc gatctttaca actgcaatcg
      541 gggtgtggcg tgccgggtcg tgctcaatgt caacaacggt tgcagtgaca gtctcggtaa
      601 aagaaccgaa gtgtttcagt tctgccttat actggtgtga cggggcgcgg taggttgaac
      661 ctccctttcc acgtgcctgt gtactaattc tgtgtcccat gttgactcac cttacacgat
      721 tcccagctga gagagaatct cttcagcagc gttattctct tcgaaggtaa tgattgcctt
      781 cttagtaccc ttggaagtca tcatggtgtt gatggatgcg accttcttac cgaagttctt
      841 ttccagtgct gccttgatgg actccttagt tgcttccttt ctgacgatga aagagagctg
      901 attggagttt tccaggagaa ccattgcttt ttcagttaca agcgggtgtg cgagtgccat
      961 gattatgcct ccccgagctt cttgacagcg gcctcagtcc agacagtcag tctgcctgcg
     1021 tgagttccgg gtgccagaac atttgcgttc agagaagtga ccggcatgac atcaacacct
     1081 gcaaggtttg caccagcggt gaaaccgggg gctgcggtga cgattaagac ggactttgcc
     1141 tgcttgtact tgcgtccgcg tgtctttccg cgccctgcac gaatcgcacg ggagttcttt
     1201 gcacgctcga ggtctgcacc gacgccaagg gcgattaatg ccttcttgac ttctgcggtc
     1261 tttgcgagtg tttcaaatgc atcctcaacg ataattgctg cgtcaccctc gaacttgtga
     1321 ccgcgggcgg tgacgagttc ggtgttaacg gttgctgcaa ttgcagaccg gattgccttt
     1381 gccttctctt tcttgttgat cttctcaaca aggatcttct cgacttttgg tgcgtgtgcc
     1441 gggtgaccgc ctttggtctg cgggactttt gcaccgcggg atccgttctt gatacgcggg
     1501 atctttgact caccgcgctt ggaaccccag ccttctgcag aggttctcat acctgcatac
     1561 gggtttgcac cgtacggctg atactgctcg ctctggtatg cgaggacagc tctcttgatg
     1621 aggtccgggc ggaactcttc attgaagact gccggaagct cgatctcgtg gagaacagaa
     1681 ccgttaattg cttttacatt tgctttcatc ggtcagatca cccctgtttg ctctggagac
     1741 ttacaaactc aacaacagga gtctggatgg tctgttcacc catacgggtt gcgggacgga
     1801 tacgaatcag gcgctttgcc ggacccggga tggaaccttt aatcaggaca tagttgccgc
     1861 ggacaagacc gtagtggagg aagccccctg ccgggttgat ttcgtccggg ttgtcaccga
     1921 gtttaatgat tctcttgttg aactccgtac gctgctggaa tcccatctga ccgggcatcg
     1981 ggacctgcca gcgaacgtgg tgcggagtcc acggaccaag agtaccgatg tgacgggtct
     2041 tcttggttct tgcgtgcttt ctctttctga gcgtgatgcc gaatctcttg acagctccct
     2101 ggaatccctt tcctttggtg attccggtga tgtcagcaaa ctgaccttcg gagaggatgg
     2161 agctcatctc aacgctggtg ccaagaagag aaagtgcgta gtcaaagcgt tcctgtgcac
     2221 tgccgccagc cacacggatt tccattaagt ctggaacttt cttcgggaca ccggtaagtg
     2281 tctccggctg ggtgtacatc agaaccataa cctcgacgac atctgccatt gcggcattga
     2341 tcttctcggt tgctgcagca gcattgtgtg cctttgcttt ggtgattcta ccggaaagtg
     2401 cagcaacatc ctcagaccag acttcggtaa gcgggtgttt gccgtaggtg tctttgacat
     2461 agacgcggat tgcggcaact ttcattgccg ggatttcaat aacggtcaca gggaccataa
     2521 tttccttgcc ttctgtcggg ctctttttgt gatcgtcaat cataatgacg tgggtcatgc
     2581 cggctttgta gcctgcaaag ccctgaagcg tgggctgccc gttatactct ggccaggatt
     2641 ggtacttagg tacaatgctc ttggccctct tgcgaggata gtatgcgagg gaaccggcac
     2701 gcggtctcac agatctcatg tgttaatcag tccttaatac tgtggtctct tcgttccttt
     2761 gggaatgaga gacgtctgct gtggttgtca attattaaac aaccgttctt tcaccatcag
     2821 cctgcgatat tgcaaatcgc cttcgaccat gggacgtaca ttctagtgtc ggtctccgct
     2881 ctctgtgcag atgcacagat gcttagaaga cggaaagacg ggtagcaatt gatccattag
     2941 gatatccgca tgaacgacaa cgtcttgtct gttcttgtgg tttttatgcc cgaatgacgc
     3001 atgttatatg cgcgccgcac agttgcggct ttccaagtct cacgtaagag aacctgacag
     3061 ttcacgcgct ctttggtttt cacaaagaat gggctcgtcc tgctgacgtt cgccctgcgc
     3121 gcttcatcag acctattgag gtcttaatcc tttgcgggat ttcccacaaa agggttacac
     3181 gataatgaaa acatcgacag aatctgcgtg ttttcaaacc aactggatat ccacgataca
     3241 cagcagctat gctgatatca gcctatgaag gcttcactat tattggaaga gtgagataat
     3301 aaaggtaatg gaggtgaaat gaggacagag cgcaagtgag gattacccaa aatagagatc
     3361 gccatctgtg ttgaggtata cccggatgtc ttctctgaca tgtcggcctt tgatcttttc
     3421 aggcatgtag gagctgatgc ggttaattaa ggagatgcta tcataccgga ttttgatatt
     3481 cagaggggcc gctgcctgat agataagatg cggtgcttcc ataagatcag tgcccgcagc
     3541 aaggacacag aggtctgctg catcaagggt atagagaaat tcgagagtct cttttgcacg
     3601 ccgaatattt tcgttcgcag atttttcaat tgtacagatg tttgcttttg atgtcccgag
     3661 acgatcggcg atctgctgct gggtcattcc ttgcttgcga tatctgataa cttccttctg
     3721 gcgatcggta aggagagtat ctttcatggt tattaatata aggctgttaa ctataaacat
     3781 atttggttaa agtactgtac ttttccagta cccgactgtg taatcagaat ctcgccaatg
     3841 aattggtttc ggagattacg cgcacaaatt tacaggtatt ttgataactc ctaaagagag
     3901 aatagcagcg actgatgatg aatgggttct tgtgcgtaag agatggaagt gttccttcat
     3961 gtggtgc
//
LOCUS       DALTZZ010000207         3018 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_33440,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000207 DALTZZ010000000
VERSION     DALTZZ010000207.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33440"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="O0X12_03940"
     CDS             <1..455
                     /locus_tag="O0X12_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HJK53339.1"
                     /translation="KALFTAAENMDPDLAARLKNAVRIEGTGMQIDDALAAIGNSSSV
                     YHTLPLAVFLCKRYDVPDELLAYASACGGNADTLTLLCGAFAGARYGISALPQDLVAQ
                     LERRSEFELLAEKLLNPQKPEPPAPEKTVTEKSPEEPESIYDSGWEPE"
     gene            517..1509
                     /gene="argC"
                     /locus_tag="O0X12_03945"
     CDS             517..1509
                     /gene="argC"
                     /locus_tag="O0X12_03945"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HJK53340.1"
                     /translation="MDIAIVGASGYAGGDLIRLLLTHSQANIVCATSRKLAGTPVTKD
                     HIHLKNLIDLDYTNPDVNDIDADFAFLAVPHTAAMQYALQLRERGIKTVDLSADYRLP
                     QDIYEKTYGVKHTAYFKAPYGIPELHRNEVRGADFVANPGCFPTGATLAAAPVADIAA
                     TIIYDSKSGVSGAGDSVSETTHYPNVDENIAPYKITAHRHLPEMKQEAAFLGSSAKIY
                     FTPHLLPAIRGIITTAHILFDEPVSLEEVKRRYETFYKDEPFVRLQTAKLGGVRGSNF
                     CDINFELEADGTRLVAVSAIDNLVKGAAGQAIQNMNIMCGYAETDGLRMPGMFP"
     gene            1556..2707
                     /gene="argJ"
                     /locus_tag="O0X12_03950"
     CDS             1556..2707
                     /gene="argJ"
                     /locus_tag="O0X12_03950"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HJK53341.1"
                     /translation="MQSICAVEGVTAWGVKEGKYGLALIKASGTGAAVFTSNKVRAPV
                     VNLMAERIKRGKLAGIIVNSGCANAYTGKRGYEDAVTMASIGAEALGISEKETGVAST
                     GVIGRYLDLDLIRRQSADVAGKLAHSADAEKAAAAAIMTTDTVQKHALVQREGFTVAG
                     ICKGSGMIAPNMGTMLSFVYTDAEVSAEYLHDALKSAVRRSLNRVVVDGDESTNDSLF
                     CTATGEAGRVPKKEFAAALEECCVSLAKQIAADGEGATKLLEVRVKGARREKDAEKIA
                     RAVITSPLVKSAVYGEDPNWGRVVCAVGYSGVEFDIDELSLSIGEGESETSLVRKGEI
                     TADLTKAKAAMTGKHVVFTITLESGKKEATAWGCDLTEQYVEINGKYTT"
     gene            2704..>3018
                     /locus_tag="O0X12_03955"
     CDS             2704..>3018
                     /locus_tag="O0X12_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HJK53342.1"
                     /translation="MNRQSVLMEALPYIRKFHGKTIVIKLGGHAMVDPKIMNTVIEDA
                     VLLHYVGMRVVLVHGGGPEITEKMKALGKEPKFIGGLRVTDAETLEIAQMVLAGKISS
                     AIV"
BASE COUNT          745 a          737 c          836 g          700 t
ORIGIN      
        1 ataaggccct ctttactgcg gcagagaaca tggatccgga tctggctgcc cggctcaaga
       61 atgccgttcg tattgaagga accggtatgc agatcgatga tgcccttgcc gcaatcggca
      121 actcctcttc ggtgtatcat acactcccgc ttgcggtctt cctctgcaag cgctacgatg
      181 ttccggatga actccttgcc tatgcttcgg cctgtggtgg taatgccgac acgcttaccc
      241 ttctttgcgg tgcattcgcc ggtgcccgct atggaatctc agcacttccg caggatcttg
      301 ttgcccagct tgaacgaagg agtgagtttg aactcctcgc agaaaaactt ctgaaccccc
      361 agaagcccga accaccggca cctgaaaaaa ccgtcacgga gaaatcaccg gaagaaccag
      421 aaagtatcta cgactccggc tgggaaccgg aataaccctc ttcttttttc acattcataa
      481 cagaatttaa tctctttcac aacccatact gtagtcatgg acattgcgat cgtcggtgca
      541 tccggatatg ccggaggaga tctcatcaga ctgcttctca cccactcaca ggccaatatc
      601 gtctgtgcga cctcccgtaa actcgccgga acacctgtaa ccaaagacca cattcactta
      661 aaaaatctca tcgaccttga ctataccaac cctgatgtca atgacattga cgctgacttt
      721 gcattccttg cagtccccca caccgcagca atgcagtatg ctcttcagct tcgggaacgc
      781 gggatcaaaa ccgttgatct ctccgcggat taccggcttc cgcaggacat ctatgaaaag
      841 acctacgggg tcaagcatac tgcctacttt aaagctccgt acggaattcc cgagcttcac
      901 cgcaacgaag tacgcggtgc cgactttgtt gcaaatccgg gctgcttccc aaccggcgca
      961 acccttgccg cagctccggt agctgacatt gctgcaacaa ttatctacga ctcaaagagc
     1021 ggagtctccg gagcaggcga ttcagtttca gagacaaccc actaccccaa cgtcgatgag
     1081 aacattgccc cgtacaaaat tacggcacac cgtcatcttc ccgagatgaa acaggaagct
     1141 gccttcctgg gttcttctgc aaagatctac ttcactcccc atctgcttcc ggcaatccgg
     1201 ggaattatta ccacagcaca tatcctcttt gacgagccgg ttagtctgga agaggtaaaa
     1261 agacggtatg agaccttcta caaggatgag ccttttgtcc gtctccagac tgcaaagctg
     1321 ggaggagttc gcggatccaa cttctgtgat atcaacttcg agcttgaggc tgacggtacg
     1381 cggcttgttg cggtctcggc aattgacaac cttgtcaaag gtgcggcagg tcaggccatc
     1441 cagaacatga atattatgtg cggatatgca gagacagacg gtctgagaat gcccggaatg
     1501 ttcccgtgag aacattcaag atacccagaa caatatataa tagacaggaa ttcatatgca
     1561 gagtatctgt gctgttgaag gcgtcaccgc atggggcgta aaggaaggaa aatacggcct
     1621 tgcacttatc aaagcatccg gaaccggtgc agctgttttt accagcaaca aagtccgtgc
     1681 tccggttgtc aacctcatgg cggaacgtat caaacgcgga aagcttgcag gtattattgt
     1741 gaacagcgga tgtgccaacg cgtacaccgg aaaacgcggg tacgaggatg ccgttaccat
     1801 ggcatcgatt ggtgctgagg cacttggcat ctctgagaag gagacaggtg ttgccagtac
     1861 tggagttatc ggcagatacc ttgatctgga tctcattcgc cgtcagagtg cagatgttgc
     1921 aggaaaactt gctcactctg ctgatgcgga gaaagccgca gctgctgcaa tcatgacgac
     1981 tgatacggta caaaagcatg ctttagtgca gcgtgaaggg ttcacggttg ccggtatctg
     2041 taaggggtcc ggtatgattg caccgaacat gggcacgatg ctttcgtttg tgtacaccga
     2101 tgcggaggtc tctgctgagt atcttcatga tgcattgaag agtgcagtac ggcggagtct
     2161 gaaccgtgtg gtggtggacg gggatgagag tacaaacgat tcgctttttt gtactgcgac
     2221 cggtgaggct ggtcgtgttc caaagaagga gtttgccgca gcacttgagg agtgctgtgt
     2281 ttcgcttgca aaacagattg ccgcggacgg cgagggtgca acaaagctgc ttgaggtccg
     2341 ggtgaaggga gcacgccgtg agaaggatgc ggaaaaaatt gccagggcag tgattacgtc
     2401 accgctggtg aagagcgctg tctacggcga ggatccgaac tggggccggg ttgtctgtgc
     2461 cgtcggatat tctggtgtgg agtttgatat tgatgaactg tcgctttcga ttggtgaggg
     2521 cgagagcgag acatctcttg ttaggaaggg agagattacc gcggatctca cgaaggcaaa
     2581 ggctgcaatg accggtaaac atgtggtgtt tacgattacg cttgagtccg ggaagaagga
     2641 ggcaacggcg tggggctgcg atctgacgga acaatatgtg gaaatcaacg ggaagtatac
     2701 aacatgaatc gacagagtgt gttaatggag gcacttccgt acatccggaa gttccatggc
     2761 aagacgattg taattaagct cggcggccat gcaatggttg atccgaagat tatgaatacc
     2821 gtgattgagg atgcggtgct tctccattat gtggggatgc gtgttgtgct ggtgcatggc
     2881 ggcggtccgg agattacgga gaagatgaag gctctgggta aggagccgaa gttcattggc
     2941 ggtctgcggg tgacggatgc ggagactctg gagattgccc agatggtgct tgccggaaag
     3001 atcagtagtg caattgtg
//
LOCUS       DALTZZ010000208         2823 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_33793,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000208 DALTZZ010000000
VERSION     DALTZZ010000208.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33793"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(44..649)
                     /locus_tag="O0X12_03960"
     CDS             complement(44..649)
                     /locus_tag="O0X12_03960"
                     /inference="COORDINATES: protein motif:HMM:NF021561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HJK53343.1"
                     /translation="MKIYSIDEEVDRLTRFVREKGGDAVKISADQIVTGEWVRMKCLF
                     GCNGFGRRFSCPPYTPTPAETRAALDCYSCALLVRFEGDVGETTPERLVSREMTRYVQ
                     TVMFELEEMAFYDGFYKAFGYTGHQCGWCGKCVAKEKGAVITDCKNRRKMRPSMEAAG
                     IDVYATCANVGWNLDVLECTAVEGGSVLGSPMSTVMLLLIE"
     gene            complement(646..1071)
                     /locus_tag="O0X12_03965"
                     /pseudo
     CDS             complement(646..1071)
                     /locus_tag="O0X12_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617011.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthase"
     gene            complement(1068..1442)
                     /locus_tag="O0X12_03970"
     CDS             complement(1068..1442)
                     /locus_tag="O0X12_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HJK53344.1"
                     /translation="MFVRAIDREVLDLLLEMGRSSSPDEFIVMLGSEKGVITTVYPIA
                     GTTVTTDSATIFMDMVPLGMQIAGTAHSHPNGVLYPSAADLQTFAETGQCHIIVGDPF
                     EADSWRCFDREGHEKSLEVVSS"
     gene            1483..2220
                     /locus_tag="O0X12_03975"
     CDS             1483..2220
                     /locus_tag="O0X12_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HJK53345.1"
                     /translation="MRSGKVTCPGTVTCNGQEIPYTIIYSDRSRSWAIEVKADASVIV
                     RMPQNIPPEKAKTLVETKSEWIFQQVQKYTSRAQISRRYTDGETLPFLGQDYPSIRKT
                     GDYAKAEFTDGLFYITLPDGFTESDQTALARDLIIMLYRRVGAAPLEDIINHYSPLAG
                     VDPPKLRIRMQERKWGCCTPKNGIIINVRVLLAPKIVAEYIVVHELAHLRFRHHQKTF
                     WGEVERIMPTYRDAEIILKNDGWKFVF"
     gene            complement(2278..2610)
                     /locus_tag="O0X12_03980"
     CDS             complement(2278..2610)
                     /locus_tag="O0X12_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HJK53346.1"
                     /translation="MKMIVAVIRPEKVDDVVDALEAVNVPGVTITDVRGRGEQGGICL
                     QYRAGKMQIHTLPKTKLEIVVPDKDVETIIKTIREHARTGKKGDGRIFVLPVEAAAWV
                     RTDDFITG"
     gene            complement(2630..>2823)
                     /locus_tag="O0X12_03985"
     CDS             complement(2630..>2823)
                     /locus_tag="O0X12_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HJK53347.1"
                     /translation="GLLEGNVTQFGIQILDACVAVVYSFVVTFVLAWIINKVIGLRVT
                     DDEEYVGLDIAQHGEQLTN"
BASE COUNT          761 a          789 c          568 g          705 t
ORIGIN      
        1 attctgagta tcaaaaaatt tagagctgga aatagaggga ctctcactca atcagcagga
       61 gcatcactgt cgacattgga gaacccagca ctgatcctcc ttccaccgca gtacactcca
      121 gaacatccag attccatcct acgttggcac aggttgcata cacatcgatt cccgcagcct
      181 ccatgcttgg acgcatcttt ctgcggttct tacaatccgt aattactgcc cccttctctt
      241 ttgcaacaca ctttccacac cagccgcact gatgcccagt gtacccaaac gccttgtaaa
      301 agccatcata aaatgccatc tcttccaact caaacatcac tgtttgtaca tacctcgtca
      361 tctcccgtga cactaaccgc tctggtgttg tttctcccac atccccttca aatctcacca
      421 gaagggcaca agagtaacag tcaagggctg cccgggtttc tgcaggagtt ggtgtatacg
      481 gtggacagga aaatctccgg ccgaacccgt tacaaccaaa caaacacttc attctcaccc
      541 attcccctgt cacaatttga tcagcagaga tctttactgc atcaccgcct ttttcacgga
      601 caaaacgtgt cagtctgtca acttcctcat cgatagagta tattttcatt gtgaacctct
      661 ccgtttcaat gcttcttcaa taatccttgt tgatgaatta aaggcacaac cgcagaacgg
      721 cccgatccga acaacatccg cccgaatacc acgctcccgc atctgggcaa tcagcttctc
      781 ctcctaaaac ttctgattga acccaagcgt gactacctct gggtcaatat ctgcaatcgg
      841 ccggaacata tcctccagat ctcctaaaat tgcatgatcc acacatctga gaccggagat
      901 cattttcaga cgctgttcct ctgggacaat cggtcgcggt ttatggacga cgtttcgttc
      961 ccgggcgaca atcacccaca gctcatcacc cagcttcctt gactcctcaa gataaaacaa
     1021 atgtccggga tgcagaatat caaacgtccc ggttgcaacg atttttctca cgaagaaaca
     1081 acctccaaag acttttcatg accttcgcga tcgaagcagc gccacgaatc tgcctcaaac
     1141 ggatcaccca cgataatatg acactgaccc gtctcggcga atgtctgcag atccgcagca
     1201 gacggataca gtacaccgtt tggatgactg tgtgcagttc ctgcaatctg cattccaagc
     1261 ggtaccatat ccatgaagat agtggcactg tcggtggtaa ccgtcgttcc cgcaattgga
     1321 tacaccgttg taatcacacc tttttctgat ccgagcatca cgatgaactc atcgggagag
     1381 cttgacctcc ccatttcaag aaggagatcc agaacttccc gatcaattgc gcgaacgaac
     1441 atattctatt tatgttggcg tgacggagag ataatcattc acatgcggtc cggaaaagta
     1501 acctgccctg ggacggtcac ctgcaacggg caggaaatcc cgtataccat catatacagt
     1561 gaccgcagca gatcatgggc catagaagtt aaggccgatg catctgtcat agtccgcatg
     1621 ccacagaata ttcctcccga aaaagccaaa actcttgtgg aaaccaaaag tgaatggata
     1681 ttccaacagg tacaaaaata cacatcgcgt gcccaaatat cccgcagata cactgatggt
     1741 gaaaccctcc ctttccttgg gcaggactat cctagtatcc gtaagactgg tgactatgca
     1801 aaagcagagt ttacagacgg attgttctat atcaccctcc cagatggttt cactgagtct
     1861 gatcagacag ctcttgcacg tgatctgatt attatgctgt accggagagt tggtgctgcc
     1921 ccccttgaag atattatcaa tcactattcc cctcttgcag gagtggatcc tccaaaactt
     1981 cgcatacgga tgcaggaacg aaaatgggga tgctgtactc caaaaaatgg gatcattatc
     2041 aatgttcgcg tcctgcttgc tccaaagatt gttgctgaat atatcgttgt ccacgaactt
     2101 gcccatctta gattccgcca ccaccagaaa acgttctggg gcgaggttga gaggattatg
     2161 ccgacatacc gcgacgcgga aataattctc aaaaacgacg ggtggaagtt tgtattctaa
     2221 cgtcgcaaaa attagaaaat agagattgga gataactccc tcatcgagct aacggtatta
     2281 tccggtgatg aagtcatcgg tccgcaccca tgcagctgcc tcaacaggca gcacaaagat
     2341 acggccgtcc cctttcttcc ccgtccgtgc atgttcacgg atagtcttga tgatagtttc
     2401 aacatctttg tctgggacca cgatctccag ctttgttttg ggcagggtat gaatctgcat
     2461 cttgcctgca cggtactgca ggcagatacc tccctgttca ccacgtccgc ggacatcggt
     2521 aatggtaact cccggaacat ttactgcttc aagggcatca accacgtcat ctaccttttc
     2581 gggtctgata actgctacaa tcattttcat tctgcatcat ctcctccatt tagtttgtca
     2641 gctgctcacc gtgctgtgcg atgtcgagac caacatactc ctcatcatca gtaacccgca
     2701 ggcctataac tttgttgata atccatgcaa ggacaaaggt cactacaaag gagtagacaa
     2761 ctgcaacaca ggcatccaga atctgaattc caaactgtgt tacatttcct tcgagaagac
     2821 cac
//
LOCUS       DALTZZ010000209         2582 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_34129,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000209 DALTZZ010000000
VERSION     DALTZZ010000209.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34129"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="O0X12_03990"
     CDS             complement(<1..524)
                     /locus_tag="O0X12_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HJK53348.1"
                     /translation="MAYYNEKIETLPKAELEKLQYQELKVLVERLYATSVFYHDKMKV
                     AGVLPSDIKSIADIVKLPTMKKTDLRDNYPDKLVCCPKEDLVRYHVSSGTTGKPTVVA
                     YTKNDVELWSEAVARSLVSCGLGRGDVLQVAYGYGLFTGGLGLHYGGEMLGATVIPAS
                     TGNSERQIELIQDLG"
     gene            complement(527..1813)
                     /locus_tag="O0X12_03995"
     CDS             complement(527..1813)
                     /locus_tag="O0X12_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HJK53349.1"
                     /translation="MFWNEKMETLRGKELQELQLKRLKETVTRTQNIGFYQKMFAEAG
                     IRPEDIKTLDDLSKIPFTKKSDLRGGYPFGFLAVPMNRINRIHTTSGTTGKPTVVAYT
                     KNDLNSWSELIARNLTMVGLREGDMFQNASNYSLFTGGLGFHYGAEKIGCAVVPAGVG
                     NTKRQIEMIQDFGVNAIHCTPSYAMHLTEVAEDMHANLESLRIGVFGAEAWSENMRRD
                     LENRLNIKAYDSYGMSELFGPGVAFECPEQDGLHIWHDCYIVEIIDPKTGETLGPGEK
                     GEMVVTPLVKEAMPLLRYRTGDITMLMEDDCPCGRGQKIARLFGRSDDMLTVRGINVF
                     PSQIEHVLKNIPEVGDQFMVYIDRLNHLDEMTIEVEINKHLFSGELSDLAKLQNKIGK
                     ALHETLTLRANVQLVEPGSLPRFEGKAKRVVDRRKI"
     gene            2007..2186
                     /locus_tag="O0X12_04000"
     CDS             2007..2186
                     /locus_tag="O0X12_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53350.1"
                     /translation="MDKTGLATLILGIVLLIAGIILMWYWLPQFIQVLLGGIGIVLAV
                     AGLCGIVLGYLMIKE"
BASE COUNT          663 a          639 c          579 g          701 t
ORIGIN      
        1 cccagatcct gaatgagctc aatctgtctc tcagagtttc cagttgatgc ggggatcacc
       61 gttgcaccca gcatttctcc gccatagtgc aggccaagac cgccggtaaa cagaccatac
      121 ccataggcga cctggagaac gtcgccacga ccaagaccac aggaaaccag ggaacgtgca
      181 accgcctcag accagagttc cacatcattt ttcgtgtagg caaccaccgt tggtttaccg
      241 gttgtacctg aggagacgtg ataacgaaca agatcctctt tcgggcagca aacgagtttg
      301 tccggatagt tatcacgcag atccgtcttt ttcatcgttg gaagtttgac gatatctgca
      361 atggatttga tatcagacgg gagcacgcct gcaactttca tcttgtcatg atagaaaaca
      421 gatgttgcat aaaggcgctc aacaaggact ttcagttcct ggtactgcag tttttccagc
      481 tctgctttgg gaagcgtttc aattttttcg ttatagtatg ccataattag attttcctcc
      541 gatctacaac ccgctttgcc tttccctcaa accggggaag agaacccggc tctacgagct
      601 ggacgtttgc acgaagggta agagtttcat gcagggcttt accgattttg ttctggagct
      661 tggcgagatc actcaattca ccgctgaaga gatgtttgtt aatctcaacc tcaattgtca
      721 tctcatccag atggttcagg cgatcaatgt acaccataaa ctgatcgcca acctcgggga
      781 tattcttgag aacatgctca atctgggacg ggaaaacgtt aatgccgcgc accgtaagca
      841 tatcgtcgct tctgccaaac agacgggcaa tcttctgacc gcgaccgcag ggacagtcat
      901 cctccatgag catggtaatg tctcccgtgc gataccggag aagcggcatt gcctctttaa
      961 cgagaggtgt caccaccatc tcaccttttt caccaggacc aagcgtctca ccggtctttg
     1021 gatcgataat ctctacaatg tagcagtcat gccagatatg cagaccatcc tgttcgggac
     1081 actcaaatgc aacacccgga ccaaacagct cgctcatacc ataactgtcg taagctttga
     1141 tgttgagacg gttctccaag tcccggcgca tgttttcgga ccatgcctca gcaccaaata
     1201 cccctatccg gagtgactca agatttgcgt gcatatcctc ggcaacctcg gtgagatgca
     1261 tggcatagct tggagtacag tgaatggcat taaccccaaa gtcctgaatc atctcgattt
     1321 gccgttttgt attaccaaca ccagctggaa caaccgcaca accgatcttt tctgcaccat
     1381 aatggaatcc aagaccacca gtaaacagag agtagttgga agcattctgg aacatgtcgc
     1441 cttcgcggag tccgaccatc gtcagattgc gggcaatcag ttccgaccaa ctgttcagat
     1501 catttttggt atatgcaaca accgtcggtt taccggtagt accggacgtt gtgtgaatcc
     1561 gattgatgcg gttcatcgga accgcaagga acccaaacgg atatccgcca cggagatcgg
     1621 atttcttggt aaaagggatc tttgacagat catcgagtgt tttgatgtct tctggacgaa
     1681 tcccggcctc agcaaacatc ttctgataga aaccgatatt ctgcgttcgt gtaacagttt
     1741 ccttcaatct tttcagctga agttcctgca gctctttccc acgcagggtt tccatttttt
     1801 cattccaaaa cataaggcaa tccttacaat tcctgtgcag tcaggccccg tgacactcca
     1861 tgataccatg tatcatgtca gggcttagca tcccactatg tatgttttct gtaaacataa
     1921 ggattatcat aagcagacag gatctctccg atcaattctt ccaaaactaa aagtaaggtt
     1981 attaggattc acgggcaata tggtgtatgg acaaaacagg tcttgcaact ctgatccttg
     2041 gtattgttct actaattgcc ggtattattc tgatgtggta ctggctgcct cagtttattc
     2101 aggttctgct tggcggtatc ggaatcgttc ttgcagttgc cggtctttgt ggaattgtgc
     2161 ttgggtactt aatgatcaaa gaataacctc ttttttatgc agacatttcc gcttgatgga
     2221 cgttggttta acttggaatt aagcgtatcc tgtggccagg cgtttcgatg gagaaagtac
     2281 ggtaacacct ggtatgctcc atctccctat ggagaaaacg tagtctggaa aatacagcag
     2341 gaaaaggatc ttctcagata tgatggaatg agtgagagag aacttatcca ttacttttca
     2401 ctggatcata atcttgccgg aatcttatcc agtattgatc gcgatccgct gattcatgat
     2461 gcaatatccc gatgccgagg gttacgaatt atgcgccaag atccctggga gtgtctcata
     2521 tcctatatct gctccagctg tgccaacatc cctgggattc aaatgagaat agaaaatctt
     2581 gc
//
LOCUS       DALTZZ010000210         3052 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_34308,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000210 DALTZZ010000000
VERSION     DALTZZ010000210.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34308"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="O0X12_04005"
     CDS             complement(<1..809)
                     /locus_tag="O0X12_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HJK53351.1"
                     /translation="MEFGWVALVIICVIVYAVVCWEIKKRNFHPEIFAFMGPCLMVRT
                     SRTGIFDVLARFRRTFLVYGTLGVVVVAACSVLMTVMIIVTAYLTMLIQPDPSPIIQP
                     QNLLLIPGVNDFVPSTFAVWFALVFAVVIHEFGHGLLSRVEKIRVKYTGILALVIPIG
                     AFVEPDEEEIEKSPLATKLRMFAAGITNNLVVGAICILALILLLGMVVPGTVPFVQGV
                     YEGYPADLAGMQPGTVILAIDGVPVANTTDISTLLAGTIPGQTIQVLGEYKG"
     gene            complement(957..1598)
                     /gene="rnhB"
                     /locus_tag="O0X12_04010"
     CDS             complement(957..1598)
                     /gene="rnhB"
                     /locus_tag="O0X12_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HJK53352.1"
                     /translation="MKICGVDEAGKGSVLGPMVTAGVLVLDTAELVGLGLRDSKKLTP
                     HRREELYEEITCRWKTAVVVRTPAEIDARSGSMNEFTASCHAAVIQSLVPDVVYVDAC
                     DVNAVRFGSHVRELSGMTDLQMISEHKADDKFLIVGAASIVAKVTRDRLVTELSNEFG
                     DIGSGYPSDPVTIRFLETYLRENGCPPSCARKTWKTVDEISARMSQKALTDFI"
     gene            1658..2314
                     /locus_tag="O0X12_04015"
     CDS             1658..2314
                     /locus_tag="O0X12_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HJK53353.1"
                     /translation="MYLIIVGLGGIGRSLAGIASENGHSVVVIDRDEERCAEILTQYD
                     LLAIAGNATDKAVLEDAGVDRADALVATTSDDALNLMACWLAKRYNVRTLVSIVNQKE
                     HSELFKEVGVRISENPDEIVARSLYVWSENPNTQLLASIAGGSIFEIKVSEGAQGVNK
                     TVREVSDVKDMLYIAIQRNGKLIIPSGNVTFQPDDVITVFTKKEAEGRSVEYMDSLFH
                     "
     gene            complement(2381..2857)
                     /locus_tag="O0X12_04020"
     CDS             complement(2381..2857)
                     /locus_tag="O0X12_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane domain-containing protein"
                     /protein_id="HJK53354.1"
                     /translation="MSDVATATKELASASAALVNAEKEMVNAALIKTKSNLDTIDQNL
                     ANLNAKGWNDNRVILLSTSKETAQNLYNTAKSAYDASTSSVSSSLKSQSLEALNKSTS
                     TLETSNALWEEAQKSPLDAVGPYAMYIVIGVVAILVIVGAIIVIRRRNENDWDELG"
BASE COUNT          864 a          751 c          680 g          757 t
ORIGIN      
        1 cctttgtact caccgagtac ctgaattgtc tgaccgggga tcgtccctgc gagaagcgta
       61 gagatgtccg tggtatttgc aacagggaca ccatcaattg caagaatcac cgttccgggc
      121 tgcatacctg caagatctgc aggatatcct tcatacaccc cctgcacaaa cggcactgtt
      181 cccggaacaa ccattccaag caacagaata agcgcaagaa tacagatcgc accaaccacc
      241 agattgtttg taatgcccgc agcaaacata cgaagttttg ttgcaagagg agacttttcg
      301 atctcctctt cgtcgggttc aacaaaagca ccaatcggga ttaccagagc caggattcca
      361 gtatatttta cacgaatttt ttccacacgg gaaagcaaac cgtgaccaaa ctcatggata
      421 accaccgcaa acaccagtgc aaaccacact gcaaacgtgg aagggacaaa atcgttcacc
      481 cccggaataa ggagtaaatt ctgcggctga ataatcggag aaggatcagg ttgaatcagc
      541 atcgtcagat atgcggtaac gataatcatt accgtcatca ggacactgca ggctgcaaca
      601 acaacaacac caagcgttcc gtacaccaga aatgtcctgc gaaaacgtgc cagtacatca
      661 aagatacccg tccgagatgt ccgaaccatc aggcacggcc ccataaaagc aaatatctcc
      721 gggtggaaat tacgcttttt aatttcccag cataccactg cgtacacaat cacgcagatg
      781 atcaccaggg caacccatcc gaactccatt gttctgtatt gttctgcggt gaaaggctaa
      841 gaagttgatc gtaggtattc agctgaatac ccgtgaacga aaacagaaga cggagatgag
      901 aagagatcac atcctgcaat gctcatcgaa cagtttccaa tccaagctgt atccaatcag
      961 ataaaatccg tgagagcttt ctgagacata cgggcactga tctcatcaac cgtcttccat
     1021 gtcttgcggg cacacgacgg tgggcatccg ttctcacgaa ggtacgtttc cagaaaccgg
     1081 attgttacag gatcagatgg atagccgctg cctatatcac caaactcatt ggaaagttca
     1141 gtaacaagcc gatcacgagt aacttttgcc acaatacttg cagctcccac aatcagaaat
     1201 ttgtcgtccg ccttatgttc tgagatcatc tgaagatctg tcataccaga caattcacgt
     1261 acatgcgatc cgaatcttac tgcattgaca tcacaggcat ccacatatac cacgtccggc
     1321 acaagagatt gtatcactgc agcatgacag gaagcagtga actcattcat tgatcctgac
     1381 cgggcatcga tctctgccgg agttcgtacc acgacagccg ttttccaccg gcatgtgatc
     1441 tcctcataga gctcttcgcg acggtgaggc gtcagcttct tggagtcacg aagaccaaga
     1501 cccacaagtt ctgcggtgtc taaaaccagc acaccggcag ttaccattgg cccgagaacc
     1561 gaaccttttc ccgcttcgtc aacaccgcag attttcatca gatcaatatc tatcacaggg
     1621 aataggtata ataccccacg atcccaaacg attcagcatg tatctcatca tcgtaggtct
     1681 tggcggcatc ggcagaagtc ttgccggcat cgcctccgag aatggtcaca gcgtggttgt
     1741 cattgatcgt gacgaggaac gttgtgctga aatccttacc cagtatgatc tccttgctat
     1801 cgccggaaac gccacggata aagcagtact cgaggacgca ggcgttgacc gggcagatgc
     1861 tcttgttgca acaacaagtg atgatgccct taatttgatg gcttgctggc ttgcaaagcg
     1921 ctacaatgtg agaacgcttg tctccatcgt caaccagaag gaacattcgg agctgttcaa
     1981 agaggttggt gtccgtatca gcgaaaaccc tgacgagatt gttgcccgca gtctgtatgt
     2041 ctggtcggaa aaccccaata cccagctgct cgcatccatt gctggcggaa gtattttcga
     2101 gatcaaagtc agcgaaggag cacagggggt taataaaaca gtacgtgagg tatctgacgt
     2161 gaaagatatg ctgtatattg cgattcagag aaacgggaaa ctcatcattc cttccggaaa
     2221 tgtcaccttc cagccggatg atgtcatcac agtctttacc aaaaaagaag cggaaggaag
     2281 atctgtggag tatatggaca gcctcttcca ctgacgaaaa atcatttttt attagaactg
     2341 tatcaattgg aaaaaaaggt gtcagaggaa aatgggatga ttatccaagt tcatcccagt
     2401 cgttttcgtt tctgcggcga atgacgataa ttgcaccaac aattaccaaa attgcaacaa
     2461 caccgataac aatgtacatc gcatatgggc ctactgcatc aagtggggac ttctgcgctt
     2521 cttcccagag ggcatttgat gtctcaagcg tactggtcga tttgttcagt gcctcaagcg
     2581 actgactctt caaggagctg gatacagaac tggtcgaggc atcgtatgca gattttgcag
     2641 tgttatagag attctgcgca gtctctttag atgtggacag gaggattacc ctgttatcgt
     2701 tccatccttt tgcatttagg tttgcaaggt tctgatcaat tgtatcaaga ttactttttg
     2761 tcttgatcag agcggcattg accatctctt tttccgcatt cactaaagct gcacttgccg
     2821 acgcaagctc cttggtcgca gtagcaacat cactcatatg attttcagca gaatttagat
     2881 catctcttgc aatctccagt ttggagataa tgttagagac atcaacaccc agtttagaaa
     2941 tttcagtaat ttgggtatcg agatcattta ttgaggtact gatttgttct atctgttccg
     3001 gaaggttatc ggggttatat accaattgtg caggagattc atatgttgat ac
//
LOCUS       DALTZZ010000211         2319 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_34528,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000211 DALTZZ010000000
VERSION     DALTZZ010000211.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34528"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="O0X12_04025"
     CDS             complement(<1..643)
                     /locus_tag="O0X12_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HJK53355.1"
                     /translation="MKFSREIELRGHIVDSGILAKVMDCVVEYNGDFETEEFTLGRQK
                     ADPSYARMQISAETPEQLTQIISELRRLGVLVTGEAEVTLKNVIKAKVAPEGFYSTTN
                     HPTYIHYEGEWIPVENMKMDALIRVDTKNRTASCAVQGKLLPGDFVVVGEEGVRVDFP
                     ERPREIGVFEFMGGDVSSERPSKTMIRQIAKELLQIKASGKKVALVGGPAIIHT"
     gene            complement(807..2162)
                     /gene="gltA"
                     /locus_tag="O0X12_04030"
     CDS             complement(807..2162)
                     /gene="gltA"
                     /locus_tag="O0X12_04030"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HJK53356.1"
                     /translation="MDRDPKERIADFGEVDLGFTEEEVRAEAARCIQCKKPKCVNGCP
                     VGIDIPAFIHACEIGAFREAARIIKKDNMLPAICGRVCPQETQCQGECVLGNKDTPIS
                     IGHLERFVADWERREGIETPVRLPATGKKVAVVGSGPAGLSAAAELARLGHAVTVFES
                     LHEAGGVLMYGIPAFRLPKDIVQTEIEQVKKLGAEIKTNYVVGRSVPVAELLSYDAVF
                     LGTGAGLPYFMGLPGENLCGIYSANEFLTRVNLMGANKFPEKDTPVKKGKKVVVIGGG
                     NVAMDAARVARRMGSEVTLMYRRGEADMPARLDEVRHAKEEGIEFMCCTNPVRFIEGE
                     NKAVAGVEAVKMELGSPGDDGRRSFAVIENSNFTVEADVVIEAIGQGPNPLLLRMLPE
                     LARNKNGSVAVNCLGQTSIPKVYAGGDVATGAATVILAMGTAKDAARAIDKMLRGASQ
                     I"
     gene            complement(2163..>2319)
                     /locus_tag="O0X12_04035"
     CDS             complement(2163..>2319)
                     /locus_tag="O0X12_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HJK53357.1"
                     /translation="MKFACVDGPEFDAHQVDWDDLLARQRMYIAEEKQSLEYHEAHHE
                     CRCRKVE"
BASE COUNT          428 a          685 c          622 g          584 t
ORIGIN      
        1 cggtgtgaat gattgcaggt ccgccgacga gggcaacttt ctttccggat gctttgatct
       61 ggagaagttc ctttgcgatc tggcggatca tggttttgct cggccgctca gaggagacgt
      121 cgcctcccat gaactcgaag acgcctatct cacgcggtcg ttcggggaag tccacgcgga
      181 caccctcttc tccgacgacg acgaagtctc cggggaggag tttgccctgt actgcgcagg
      241 atgcggtccg gttctttgtg tcaacgcgga tgagtgcatc cattttcatg ttctctaccg
      301 gaatccattc cccttcgtag tggatgtagg tcgggtggtt agttgtggag tagaagcctt
      361 ccggtgcaac ttttgctttg atgacgtttt tgagggtgac ttctgcttct ccggtgacca
      421 gaacaccaag acggcgcagt tcgctgatga tctgcgtgag ctgttctggg gtttccgcgg
      481 agatctgcat ccgtgcgtaa ctcgggtcgg ctttctgcct gccgagggta aactcctcgg
      541 tttcaaagtc tccgttgtac tcgacaacgc agtccatgac tttggcaaga attcctgagt
      601 caacgatgtg acccctcagt tcgatctccc gcgagaattt catggatata catgggttgt
      661 gcgatgagat gaatttctcg cagtggttcc cgttgttttg gtgtggatgg gttcactctc
      721 tgatctgcgg acacctgttt cccgcatatc tgttacggtc tgggatggag acgggacgga
      781 cacctgccgc gaaaaaaaga attttgttag atctgtgatg cgccgcggag catcttatcg
      841 attgcccgtg cagcgtcctt tgcagtaccc attgcaagga ttaccgttgc ggcaccggtt
      901 gccacatcgc ctccggcata gaccttcgga atcgatgtct ggcccagaca gttcacggca
      961 acactgccgt tcttgttgcg ggcgagctcc ggcagcatgc gcaggagcag cgggtttggt
     1021 ccctgaccga ttgcttcgat gacaacatcg gcctcaaccg tgaagttgct gttttcgatc
     1081 accgcaaagg aacgtctgcc gtcgtcaccc ggactgccga gctccatttt gaccgcttca
     1141 acacctgcga ccgctttgtt ttctccttca atgaagcgga cagggtttgt gcagcacatg
     1201 aactcgatac cttcctcttt tgcgtggcgg acctcgtcca gccgtgcagg catgtcagcc
     1261 tcaccgcggc ggtacatcag cgtgacctct gaacccatcc ggcgtgccac acgtgccgca
     1321 tccatggcaa cgtttccgcc tccgatgacg acgaccttct tgcccttctt aacgggtgtg
     1381 tccttttccg ggaacttgtt tgcacccatc aggttcactc tggtcaggaa ctcgtttgcc
     1441 gagtagatgc cgcagaggtt ttcgccgggc agacccatga agtagggaag gcctgctccg
     1501 gttcccagaa acaccgcatc ataactgagc agctctgcga ccggtacact gcggccgacc
     1561 acatagttcg tcttgatctc tgcaccgagt tttttcacct gttcaatctc ggtctggacg
     1621 atgtctttgg ggagacggaa cgccggaata ccgtacatca gcacacctcc cgcttcatgc
     1681 agggactcaa acaccgttac cgcgtggccg agccgggcaa gctctgcggc tgcggaaaga
     1741 cccgcaggtc cggatccgac cactgccacc ttctttccgg tcgcaggaag ccgtaccggt
     1801 gtctcgattc cttctcttct ctcccagtcg gcaacaaacc gctccagatg tccgatggag
     1861 atcggggtgt ccttgtttcc aagcacacac tcgccctggc actgggtctc ctgcgggcac
     1921 acacggccgc agatggccgg aagcatgttg tcctttttga tgattcttgc agcctcgcgg
     1981 aacgcgccga tctcgcaggc gtggatgaac gccgggatgt cgataccgac cggacagccg
     2041 tttacacatt ttggtttttt acactgaata cagcgtgcag cctccgcccg gacctcttcc
     2101 tccgtaaatc cgagatcaac ttcaccgaag tctgcaatcc gttcctttgg atctctgtcc
     2161 atttactcca ccttcctgca gcggcactca tggtgcgctt cgtggtactc aagcgactgt
     2221 ttttcctctg ctatgtacat ccgctggcgt gccagcagat catcccagtc cacctggtgt
     2281 gcatcgaact ccgggccgtc aacacaggcg aacttcatc
//
LOCUS       DALTZZ010000212         1929 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_34662,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000212 DALTZZ010000000
VERSION     DALTZZ010000212.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34662"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="O0X12_04040"
     CDS             complement(<1..1089)
                     /locus_tag="O0X12_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HJK53358.1"
                     /translation="MGIENRSNCHVLVIGSGGAGIRAAVEAAASGETIIVSKTITGKG
                     GCTVMAEGGYNAVLRSADSVSTHYEDTMKGGAFLNDPALVEVLAREAPDRIRDLFAWG
                     AVFDLTCENEIAQRPFGGQRFPRTCYAGDHTGHEMVMTLLEKLRGSPVEIRDELTVID
                     LLKDGNTVSGAITCDREGELGVITADAVVLATGGAGQVYDTTTNSTAGTGDGYALGYR
                     AGAELIDMEQVQFHPTGAVYPYDTRGRLITEAVRGEGGILLNTKGERFMEKYDPARME
                     LSTRDVVARSIATEVLEGRGTEHGGVWLDVTHLPREQIETRLPLMLEQFLQFGVDIRT
                     QPMEVAPTAHHFMGGLRITPEAHTTLPHL"
     gene            complement(1177..>1929)
                     /locus_tag="O0X12_04045"
     CDS             complement(1177..>1929)
                     /locus_tag="O0X12_04045"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HJK53359.1"
                     /translation="DHKFVMNSPGTAEVQPGNHLVSIQSSEYFTWSDEVSVSSGETTM
                     VNAVMQFYKAPGSIGITSSMPEVDVYIDDMYYASVKSGTVTIPNLSPGEHDVRVVKAG
                     YHDFTTKVQVISEKIVGVYSNQVKDERQAGIRVHSEPAGAVVYLDDDYVGITQSGNEW
                     LQISGVYPGSHTLSFVKDGYTVSTITKDYSAGTVENIRVTLQPIPIETTPFPTETAVV
                     PTETVPPAPTKTPAPLMGVILLGAAGVLLSRR"
BASE COUNT          488 a          521 c          467 g          453 t
ORIGIN      
        1 cagatgcggc agcgtggtat gggcctcagg agttatccgc agacctccca tgaagtgatg
       61 ggcagttggt gcaacctcca ttggctgagt acggatgtcg acaccgaact ggagaaactg
      121 ttccagcatc aaaggcagac gggtttcaat ctgttctctt ggcagatggg ttacatccag
      181 ccacacacca ccgtgttccg tcccgcgacc ctcaagaacc tcagtggcga tcgatcgtgc
      241 aacaacatcg cgggtagaaa gctccattct ggctggatca tacttttcca taaaccgttc
      301 accctttgta ttcaaaagaa tgccaccctc cccacgcaca gcctcggtta tcagacgacc
      361 gcgggtatcg tagggataca ccgcaccggt cggatgaaac tgtacctgct ccatatcgat
      421 aagttcagca cccgcacgat atccaagagc gtaaccatca ccggttcctg cggtggagtt
      481 ggtcgtcgtg tcatagacct gacctgcacc gccggttgca agtaccacag catcggcggt
      541 gatgacaccg agttctcctt cccgatcaca ggtaattgct cctgaaacag tgttgccgtc
      601 tttgagaaga tcgatgacag tgagttcatc acgaatttca accggagaac cacggagttt
      661 ttcaagaagt gtcatcacca tctcatgacc cgtatgatct ccggcataac aggtacgggg
      721 gaatctctga ccaccaaatg gacgctgggc tatctcgttc tcacaggtga gatcaaacac
      781 tgccccccat gcaaacagat cacgaatacg atccggggct tcacgggcaa gaacctccac
      841 aagagccgga tcattcagga aggcccctcc tttcatcgta tcctcataat gcgttgaaac
      901 ggagtcagca gatcgcagca cagcgttgta tcctccttcc gccataaccg tacaacctcc
      961 ttttccggta atagtctttg atacgatgat cgtctcgcct gatgctgccg cctcaactgc
     1021 agcacgaatc cctgcaccgc cggatccgat gacaagtaca tggcaattac ttctgttttc
     1081 tatgcccatg gaacgtcccc tctgtgtgta cttatagtag tgcgggaaag ataaaatcgt
     1141 gtatggaggc ggagcacacg aaaaaagaag aggatcctat ctgcgggaaa gcaaaacacc
     1201 tgcagcaccg aggagaatta cacccataag cggggcaggt gtcttggtag gtgcgggagg
     1261 taccgtctca gttggaacaa cagccgtttc agtggggaag ggagttgtct cgataggaat
     1321 cggttggaga gtgacacgga tattttcaac agttcctgca ctgtaatctt tggtgatggt
     1381 tgaaaccgta taaccgtcct ttacaaaact cagcgtatga gaacccggat agacgcccga
     1441 tatctgcaac cactcattgc cggactgtgt aattccaaca tagtcgtcgt ccagatacac
     1501 cacagcaccg gcaggttcgg aatgaacacg aatacctgcc tgtcgttcat ctttcacctg
     1561 atttgaataa acgcctacga tcttttcgga aattacctga actttggtgg taaagtcatg
     1621 gtatcctgct tttaccacgc ggacgtcatg ttctcccggg gaaaggttcg gtatggtcac
     1681 cgtaccggac ttgacactcg catagtacat gtcatcaatg tacacatcaa cctccggcat
     1741 cgaggacgtt attcctattg aacccggggc tttgtagaac tgcattaccg cgttgaccat
     1801 cgttgtttca ccggaactta cagagacctc atccgaccag gtgaagtact ccgaggactg
     1861 gatactgacc agatgattac ctggctgaac ttcagcagta ccgggtgagt tcatcacaaa
     1921 tttgtgatc
//
LOCUS       DALTZZ010000213         4828 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_34988,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000213 DALTZZ010000000
VERSION     DALTZZ010000213.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4828)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4828)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4828
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34988"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="O0X12_04050"
     CDS             <1..584
                     /locus_tag="O0X12_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HJK53360.1"
                     /translation="WQGMGYNRRALNLQKLAVRVQEEFGGILPEDPKTLATLPGIGPA
                     TSCSIAAFAFNRPVVFIETNIRRVFIHYFFSDTAVVDDRDLFPLVEACLLPGRTREWY
                     WALMDLGTALKTSVPNPNRRSRQYVKQSTFDGSDRQIRGALLRLLLNGDAEDAETLAK
                     AAGASHDRVSRILEEMCREGFFVQEEGGAYRIR"
     gene            complement(606..1073)
                     /gene="pyrI"
                     /locus_tag="O0X12_04055"
     CDS             complement(606..1073)
                     /gene="pyrI"
                     /locus_tag="O0X12_04055"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HJK53361.1"
                     /translation="MKRSVSEGIVISPIKNGTVIDHITPGEGLTVIRILGIQDGTNVT
                     FTVATNVTSSRGGRKDMVKIENRELVKSEVDKIALIAPDAKISIIRDFKVIAKKPVEV
                     PSDIVGVIKCPNPNCISNTREPAQSKFVVHPRGFRCQYCDTIISREMDIGDYI"
     gene            complement(1070..1984)
                     /gene="pyrB"
                     /locus_tag="O0X12_04060"
     CDS             complement(1070..1984)
                     /gene="pyrB"
                     /locus_tag="O0X12_04060"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HJK53362.1"
                     /translation="MTHDQKNILSVRDMDREEIDALLTAAAKFDQREFTGHELDGKIL
                     AVLFFEPSTRTRMSFSSAMMRLGGKILNLGSVEASSIAKGETLSDTIRVISGYADAIV
                     LRHPKEGAARLASEVATVPVINGGDGAGQHPSQTLLDLFTIRKSMRLENIDVGLQGDL
                     RYGRTVHSLADALTKYNNIRLHTIAPEGLDLPNHLKQDLTDAGMEIIVHENIEEALPE
                     LDVLYVTRLQRERFPDPAAFANFAATYRITPDLLERAKPSMIVLHPLPRVDEIDPAVD
                     SLPCAKYFQQAHYGVPVRMAMLHEVMSS"
     gene            2101..2904
                     /locus_tag="O0X12_04065"
     CDS             2101..2904
                     /locus_tag="O0X12_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HJK53363.1"
                     /translation="MIHIIPKPTDTPDDNSTGMVMDELRRMQVPFSVLDLSAVDPFHL
                     PVEGETIWACGIRQDGVQFELLKALELCNHVINSPEAIATSASKVTTTAKLIHAGAPS
                     PDTCFTNDKRIVAEFLAAHGGKAVYKPVYGFDGNGIYLFNSVDQITEAAPYYVQEYVR
                     NDRDYRIFVIGNKAVGAIKRESAHLTHNIHQGGSGCAVEISPEMREAAESAAEAVGID
                     YCGVDLLPLDDGGFTVLEVNGTPNWHCMKAPIPRLLAEYLVEQEKEGRR"
     gene            complement(2927..3217)
                     /locus_tag="O0X12_04070"
     CDS             complement(2927..3217)
                     /locus_tag="O0X12_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0058 family protein"
                     /protein_id="HJK53364.1"
                     /translation="MHKEELIELHQIFYDIKEYFESINPDLKFPQYTALKIHPDQVNR
                     SKLEHKYAIFILGSELAASMKEFETISSRGIPTRMKELAEKTLKEMESEREH"
     gene            3363..>4828
                     /locus_tag="O0X12_04075"
     CDS             3363..>4828
                     /locus_tag="O0X12_04075"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HJK53365.1"
                     /translation="MKSIYVIGHRHPDTDSICSVIGYAAYLNKQGSGQYIAARCGDLN
                     AESRYALEKFGVEAPELILSVEPSVADIPFTYPQSATANTPTIDVIEAMDANDLRNLP
                     ITDGAGKLIGLVSEHGLARAYVSTIKRENLTMSPMPVDILARILSAKICVKKHDVLEG
                     SVYISIDALHVILSRITEKDIAIVGDDEPTQLALVSAGIAALIIADSAPVGERLLSAA
                     EARGVTVLSTDVDAFGVAKLIHLTLPAEMIMATDVPTVHMNDTLEYVKELVSNSKYRT
                     ACVVDDNGMLISTISRNSLMEEIAKSVILLDHNEYSQAVEGIETADIIEIIDHHRLGA
                     MATLRPIRFDMEPVGSTSTIVTRRFMESGIRPEKGVAGTLLSGILSDTLGLKMSTTTK
                     LDEEAVSFLAEIAEVDPVAYAQELIAEGMSLSGVSQKELLDRDTKEYTLSGKRVVVAQ
                     ILTPSYEYARSAVDDIYAALREKMQEPNAPDMYIALYT"
BASE COUNT         1188 a         1236 c         1273 g         1131 t
ORIGIN      
        1 cctggcaggg aatgggatac aaccggcgtg cactgaatct tcagaaactt gccgtccgtg
       61 ttcaagagga gttcggagga attcttccgg aagatccaaa aacccttgca acactgccgg
      121 gaatcggccc tgctacctcc tgttcaatcg cagccttcgc cttcaaccgg ccggtggtat
      181 tcatcgagac caacattcga cgcgtcttca ttcactattt cttctcagac accgcagtag
      241 tggacgaccg tgatcttttc ccgctggttg aggcctgcct tttgccggga agaacccgcg
      301 aatggtactg ggcactgatg gatctcggaa ccgcactcaa aacctctgtc cccaacccaa
      361 accgccggag caggcagtat gtaaagcagt ccacgtttga cggatcagac aggcagattc
      421 gcggagcact gctccgtctg ctgttaaacg gggatgcaga ggatgcagaa acacttgcaa
      481 aagcggcagg ggcatcccac gacagggttt ccaggatact ggaagagatg tgccgcgaag
      541 gattttttgt acaggaagag ggcggtgcct accggatacg gtaaccccgg cacagcaaaa
      601 aaagattaga tgtagtctcc gatgtccatc tcgcgggaga tgatcgtatc gcagtactga
      661 cagcggaaac cccttgggtg cacaacaaac ttgctctgcg ccggttcgcg ggtattgctg
      721 atacagttcg ggttcgggca cttaatcaca cccacaatat cagacggaac ctccaccggt
      781 tttttggcaa tcaccttgaa atcccggata atactgatct ttgcatccgg agcaatcaga
      841 gcaatcttgt ccacctcgga cttcaccagt tcgcggtttt caatctttac catatccttc
      901 ctgccgccgc ggctgctggt cacgtttgtc gcaaccgtaa acgtcacatt cgttccgtcc
      961 tgaataccaa gaatacggat aaccgtcagc ccctcaccag gggtaatgtg atcgatcacc
     1021 gtaccatttt tgatagggga gatcacaatt ccttccgaaa ccgatcgctt catgaactca
     1081 tcacctcatg caacattgcc attctcaccg gcacaccgta atgcgcctgc tggaagtact
     1141 ttgcacacgg caggctgtca accgccggat cgatctcatc cacccgggga agcggatgaa
     1201 gaacaatcat cgacggcttt gcccgctcca aaagatccgg cgttatccgg tacgttgccg
     1261 caaagtttgc aaacgcggcc ggatccggga accgctcacg ctgcagccgg gtaacataca
     1321 gcacatcaag ctcgggaaga gcctcctcaa tgttttcatg cacaataatc tccatgccgg
     1381 catcggtgag atcctgcttc aaatgattcg gcagatcaag gccttccgga gcaatcgtat
     1441 gcagacggat gttattgtac tttgtcagcg catcagcaag ggagtgaacc gttctgccgt
     1501 aacgaagatc accctgaagc ccgacgtcaa tattttcgag ccgcatcgac ttgcgaatcg
     1561 taaacagatc cagaagcgtc tgtgaggggt gctggccagc cccgtcacca ccgtttatca
     1621 caggaaccgt tgctacctcg cttgcaagcc gggcagctcc ctcctttggg tgcctgagga
     1681 caatggcgtc cgcatatccg gagatgacgc ggatggtgtc cgaaagtgtt tccccttttg
     1741 caatcgatga cgcctccaca ctgccaagat tcagaatctt tccgccaaga cgcatcatcg
     1801 ccgacgaaaa agacattctg gtccgggtac ttggttcaaa aaagagcaca gcaagaattt
     1861 ttccatcgag ttcatgcccc gtgaactcac gctgatcaaa cttggctgca gcagtaagaa
     1921 gagcgtctat ctcttcgcgg tccatgtccc gtacagacag gatatttttt tgatcatgtg
     1981 tcatgtgaga tcgaccaact ctggtgtatc tgtatatgtc cggtgaggga taaataatgg
     2041 tcgtctcgta cattcccaaa agaaaccctt atcgttgtcg ccctgcatac agaagtgaca
     2101 atgattcata taattcccaa accgaccgac acgccggacg ataactcaac cggcatggtg
     2161 atggatgagc tgcggcggat gcaggttccg ttctctgttc ttgatctttc ggcggttgat
     2221 ccgttccacc ttccggttga aggggagact atctgggcat gcggaattcg tcaggacgga
     2281 gtacagtttg agcttctcaa agccctggaa ctctgcaatc atgtgataaa ctcaccggaa
     2341 gcgattgcaa ccagtgcaag caaggtcacc accacagcaa aactgatcca tgcgggagcc
     2401 ccgagtccag atacctgttt tacgaacgat aagaggatcg ttgcagagtt tcttgccgca
     2461 catgggggca aagccgtcta caaaccggtt tacggattcg atggaaacgg aatctatctc
     2521 tttaactcgg ttgatcagat caccgaggcc gcaccatatt atgtacagga gtacgtaaga
     2581 aacgatcgcg actaccgcat ctttgtcata ggaaacaaag cggttggcgc aattaaacgg
     2641 gagtcggcac atctcaccca caacattcac cagggaggat ccggctgtgc cgttgagatc
     2701 tcgccggaga tgcgggaggc agcagagtct gcggcagagg ctgtcggcat tgattactgc
     2761 ggagtggacc ttctgcctct ggacgatggg ggatttacgg ttcttgaggt caacggaacg
     2821 ccgaactggc actgcatgaa agcaccaatc ccacggcttt tagcagagta cctggttgag
     2881 caggaaaaag aaggaagaag ataatactac tatttactac tattttttag tgttcgcgtt
     2941 ctgattccat ttctttgagc gtcttttccg caagctcctt catgcgtgtt ggaatgccgc
     3001 gggaagagat ggtctcaaac tctttcatgg atgcagcaag ctcagagccg aggataaaaa
     3061 ttgcgtattt gtgctcaagt ttgctacggt tgacctgatc cgggtggatc ttcagggcag
     3121 tgtactgcgg gaacttcaga tccggattga tagactcaaa gtattcttta atatcataaa
     3181 atatctggtg cagttcgatg agttcttcct tatgcattgt gccattatga taagaggtat
     3241 agcatcataa gcatttgccc gtcataaaag tccggatgcg ggaggtaaga tgcaggattt
     3301 tcgggcgtaa tacctatctt tcaatccctc ccatatgtat agaagaataa tcaggagcag
     3361 ctgtgaagtc catctatgtt atcggccacc gccacccgga tacggacagt atctgcagtg
     3421 tgataggata tgccgcctac ctcaataaac agggatccgg tcagtacatc gccgcccgct
     3481 gcggggacct gaacgccgag tcccggtacg cgctggagaa gtttggtgtg gaagcaccag
     3541 agctgattct cagtgttgaa ccgtccgttg cggatattcc gttcacatat ccgcagagtg
     3601 caaccgcaaa tacgccgaca atcgatgtga ttgaggcgat ggatgcaaat gatctgcgga
     3661 atctgccgat caccgacggt gcaggaaagc tgatcggtct ggtgagcgag catggtcttg
     3721 cccgtgcgta tgtaagtaca attaagcggg agaacctgac gatgtcgccg atgccggttg
     3781 atattctggc acggattttg tcggcgaaga tctgtgtgaa aaagcacgat gtgctggaag
     3841 ggtcggtgta catctcaatc gatgcactgc atgtgatact ttcccgaatc acggaaaaag
     3901 acattgcgat cgtcggggat gatgaaccga cacaactggc actggtgtct gcgggaattg
     3961 cggcactgat cattgcagac tctgcgccgg tcggcgaacg gctgctctcc gctgcggagg
     4021 cccgaggcgt tacggttctt tctacggatg tggatgcgtt cggcgttgca aagctgattc
     4081 acctgacgct ccctgccgag atgatcatgg caaccgatgt tcccaccgta catatgaacg
     4141 atacgctgga gtacgtcaaa gagctggtct ccaactccaa gtaccgtacc gcctgtgttg
     4201 ttgacgataa tggaatgctt atcagtacca tctcccgcaa ctcgctgatg gaagagatcg
     4261 caaagtcggt gattctgctg gatcacaatg agtacagcca ggcggttgag ggaattgaga
     4321 cggcagatat tatcgagatc atcgatcatc accgcctggg agcaatggca acgctgcggc
     4381 cgatccggtt tgatatggaa ccggtgggat ccacctcgac gattgtgaca cgccggttta
     4441 tggagtccgg gattcggccg gagaagggag ttgccggaac actgctttcc ggtattcttt
     4501 ccgatacgct cggccttaag atgtcaacaa cgacaaaact ggatgaagag gcggtctcat
     4561 tccttgcaga gatcgcggag gtggatcctg ttgcctatgc ccaggagctg attgcagaag
     4621 gaatgtccct ttcaggggtt tcccaaaaag agctgcttga ccgcgataca aaagagtaca
     4681 cactttctgg gaaacgtgtg gttgttgcgc agattctgac accgtcctat gagtatgcac
     4741 gttcggcagt ggacgacatt tatgcagcgc ttcgggagaa gatgcaggaa ccaaacgctc
     4801 cggatatgta tattgcactg tatacgag
//
LOCUS       DALTZZ010000214         3977 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_35169,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000214 DALTZZ010000000
VERSION     DALTZZ010000214.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35169"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="O0X12_04080"
     CDS             <1..1106
                     /locus_tag="O0X12_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HJK53366.1"
                     /translation="GVPVVGPKGKLVGIVSRRDVRGVIHKMSAETVDTIMTKRPITVK
                     EDITADEAINMMYAKKVERLPVVDEKGKLIGIITMQDLLEKQQFPNANRDKSGNLRVA
                     AAVGPFDMNRAMMLAEAGADAIIVDCAHGHNMHVVQGVKDIKGSVSCDVIAGNIATAA
                     AARELVDFVDGIKVGIGPGSICTTRIVAGVGVPQVSAIANVCDIADPAGVPVIADGGV
                     KYSGDVAKAIVAGASSVMMGSMFAGTDEAPGKIIVVKGRRYKQYRGMGSLGVMTSGQS
                     SDRYFQKKGIGATKYVPEGVEGATPYVGSVTDVIYQTIGGLKSAMGYTGSKDIESMRK
                     NARFVRITAAGLKESHPHDILITDEAPNYRMGL"
     gene            complement(1202..1714)
                     /locus_tag="O0X12_04085"
     CDS             complement(1202..1714)
                     /locus_tag="O0X12_04085"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HJK53367.1"
                     /translation="MKKYLITLTFLLAAVLFAVPATAAIVSDFTYTVDPSDPLTIHFT
                     EKSTGGAGNWFWMFNDGGAVSNERNPTHTFSEEGTYLVSCVAQSSDYSEENTMRKQLT
                     ITLGGVTDDSGGSGSSSGGSSSSGDNSGGGISLPDISLGGISIPNPLDIIGEYIKLIR
                     VMIIPSNYNI"
     gene            complement(1724..2752)
                     /locus_tag="O0X12_04090"
     CDS             complement(1724..2752)
                     /locus_tag="O0X12_04090"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HJK53368.1"
                     /translation="MRKKIVVILLICALVLGCIGVVSAEGTNNSSNTSTGDGNKNNPE
                     GSISGGTDNKTNTDPPANNEANTDPPANNETNTDPPANNETNTDPPANNETNTTTTTT
                     PIPKKDLEASISANVTKGSAPLTVNFTAEVDDSLVAKYKWEVHEDEGYMDGRSVEYTY
                     KEAGKYTVTLTLTPKDTTTYSTTEITKTITVTQEKLEAIISASEEEGVAPLKITFTGT
                     AKGNPVSWEWDFDDGSSLIKNQTKVEHSFTKAGVYNVRLTIRNETGSTASTTKQIEVT
                     EKPKTTTTTATPTKTTAPTTATTTVSAVKSASLSADDSPIPNPLDIIEELIRLLKVML
                     VPENYSLA"
     gene            complement(2764..>3977)
                     /locus_tag="O0X12_04095"
     CDS             complement(2764..>3977)
                     /locus_tag="O0X12_04095"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HJK53369.1"
                     /translation="QPGEKTYISSPGIRVKRASFTAYLTNTRFVLMSNTNGMLAVAGE
                     FAVNTLKKAVRIAGELGEPGIGLTIASRDGEKEMHLIFPSMDLREMWIGKFEEIIPPE
                     QAQMFGSAAAAQQYSVTTVTPPARGNAPVKYCTVCGARNHPDDRFCAMCGKPLGETGV
                     ATEPSVPDMFGGRDEGTRQRKGKVKRGRKSSSNDYEMPRERRVKERTPRRERVPKQPK
                     VKTPYDGSIIGFLTRPADAFEYYGRESPKEAVGLFLIAGIVWAVISVVMLAFVLPMLL
                     PITTADFPIIGALQNNVLAMVLLILMLFVLWVVFVMLQGVLSGVFARIFGEDASIGET
                     TAVVMRSSLPYAVLGWIPGFGVIIAAIWSLITVIKGFSTALDMRGGSAAGCGILGIVL
                     VAIILVVLGIL"
BASE COUNT          881 a          943 c         1024 g         1129 t
ORIGIN      
        1 gcggtgtacc tgtggtaggt ccgaaaggga agctggtcgg tattgtatcc cggcgtgatg
       61 tgcgtggtgt tatccacaag atgagtgcag agaccgtcga tacaattatg acaaagcggc
      121 cgatcactgt caaggaggat attaccgcag atgaagccat caacatgatg tatgcaaaaa
      181 aggttgagcg actgccggtt gttgatgaaa aaggaaagct gatcggtatt attacgatgc
      241 aggatcttct tgagaagcag cagttcccga atgcaaaccg cgataagagc gggaatctcc
      301 gtgtggctgc agctgtcggt ccgtttgata tgaaccgtgc gatgatgctt gcagaggctg
      361 gtgctgatgc aattattgtg gactgtgccc acggtcacaa catgcatgtc gtgcagggtg
      421 ttaaagacat caaaggttcc gtctcctgcg atgtgattgc aggaaacatt gcaacagctg
      481 cggcagctcg cgagctggtg gactttgttg acggtatcaa ggttggtatt ggtcccggtt
      541 cgatttgtac aacccgtatt gttgcaggtg ttggtgttcc gcaggtgtcg gctattgcaa
      601 atgtctgtga tattgcggat cctgctggtg tcccggtgat tgcggatggt ggtgtgaagt
      661 acagcggtga tgtggcgaag gcaattgttg ctggtgcatc gtcggtgatg atgggcagta
      721 tgtttgccgg aacagatgaa gcgcccggca agattattgt ggtaaaaggc cgccgctata
      781 agcagtaccg cggtatgggc tctcttggtg tgatgacttc cggtcagtca agcgaccggt
      841 acttccagaa gaagggcatc ggcgcaacga agtatgtgcc ggagggtgtt gaaggtgcga
      901 caccatatgt ggggtctgtg acggatgtta tctatcagac tatcggcggt ctgaagtctg
      961 cgatgggtta caccggcagc aaggacattg agtcaatgcg caagaatgca cgctttgtgc
     1021 ggattactgc ggctggtctg aaagagagtc acccgcatga tattttaatt acggacgagg
     1081 ccccgaacta ccggatgggt ctctgatttt tttcattttt tgtttgcacg tttctctgtg
     1141 atccattttc cctatgcttg cttatctgca tgtgtggaat gatggatcaa aaaaagagtg
     1201 tttagatgtt gtagttgctt gggatgatca ttacacggat gagtttgatg tactcaccga
     1261 tgatgtccag cgggttgggg atgctgatgc cacccaacga tatgtccggg agactgattc
     1321 caccacctga gttatcaccg ctggagctgc taccaccaga ggagcttcca gatcccccac
     1381 tgtcatcagt tacaccgccg agcgtgatag tgagttgttt tctcatggtg ttctcttcac
     1441 tatagtcaga tgactgtgct acacaggaaa ccagatatgt cccttcttcg ctgaaggtat
     1501 gggtgggatt gcgctcattt gagactgcac ctccgtcgtt gaacatccag aaccagttgc
     1561 ctgcaccgcc tgtggatttc tccgtgaagt gaatggttag agggtcagag ggatcaacgg
     1621 tatacgtaaa gtctgagacg attgcagcag tagcaggtac tgcaaagagt acagctgcaa
     1681 ggaggaaggt gagtgttatc agatattttt tcatattttt acctcaggca agtgagtaat
     1741 tttctggaac cagcatgacc ttcagcaggc gtatgagttc ctcaatgatg tccagaggat
     1801 tggggatggg actgtcatca gcagataggg atgctgattt cacggcagag accgttgttg
     1861 tagctgttgt tggtgctgtt gtttttgtgg gagttgccgt tgtggtcgtg gtctttggtt
     1921 tctccgtaac ctcgatctgc ttagtggttg atgccgtgct ccccgtttca ttacgaatgg
     1981 tgaggcgtac attgtacact cccgcttttg taaacgagtg ctctaccttt gtttgatttt
     2041 ttatgagaga actgccgtca tcgaagtccc actcccatga tactggattt ccttttgcag
     2101 tgcctgtaaa ggtaattttc aagggagcaa ccccctcctc ctctgatgct gagatgattg
     2161 cttcgagttt ttcctgagtt acggtaatgg tttttgttat ttcagttgtg gagtacgttg
     2221 tggtgtcctt tggcgttagg gtgagagtca cagtgtactt tcctgcttcc ttataggtgt
     2281 actccactga acgtccgtcc atgtaccctt catcttcatg cacctcccac ttatatttgg
     2341 ctaccaatga atcgtcaacc tctgcagtga aattgaccgt taatggtgca gatcctttgg
     2401 ttacatttgc actgatggat gcctcaaggt cttttttagg tatgggtgtt gtcgtcgttg
     2461 tcgtgtttgt ctcgttgttt gctggtggat ctgtatttgt ctcgttgttt gctggtggat
     2521 ctgtatttgt ctcgttgttt gctggtggat ctgtatttgc ctcgttgttt gctggtggat
     2581 ctgtgtttgt cttgttgtct gtccctccag atatagatcc ctcggggttg ttcttattcc
     2641 catcaccagt acttgtatta gatgagttgt tagtaccttc tgcactcacc actcctatac
     2701 aaccgagcac cagagcgcat atcaacagta ttacgactat cttctttctc atgttcatac
     2761 acctcagaga ataccgagta ccacaaggat gatcgcaacc aagacgatgc caagaatacc
     2821 gcatcctgcc gcactaccac cacgcatatc aagtgcagta ctgaatcctt tgatcacggt
     2881 aatcagcgac cagatagccg caataattac tccaaatccc ggaatccagc cgagtaccgc
     2941 atagggcaga ctgctccgca ttacgaccgc tgttgtctcg ccaattgatg catcctcgcc
     3001 gaaaatcctt gcgaagacgc cggaaagtac gccctgcagc atgacaaaca caacccagag
     3061 tacgaacagc atcagaatca gcagaaccat tgccagcaca ttgttctgta atgccccaat
     3121 aattgggaaa tccgcagtgg ttattggtag cagcatcggc agaacaaacg caagcatcac
     3181 aactgagata accgcccaga caattcctgc tatcaggaac aatccgacag cctctttggg
     3241 actctcgcgt ccgtaatact caaatgcatc tgcaggacgg gttagaaatc cgataatact
     3301 tccatcatag ggcgttttta cctttggctg ctttggcaca cgctcgcgtc tgggagttcg
     3361 ttcttttacc cgacgttcac gcggcatctc atagtcattg cttgaagatt tacgccctcg
     3421 ttttaccttt cctttccgct gccgtgtacc ttcgtcgcgt cccccgaaca tatccggaac
     3481 cgacggctct gttgcgacgc ctgtctctcc caggggtttg ccgcacatag cacaaaaacg
     3541 atcatccgga tggtttcgtg cgccgcaaac tgtacaatac ttcacaggag catttcccct
     3601 tgcaggaggt gtcactgttg tcaccgagta ctgctgtgcc gctgctgcac tcccaaacat
     3661 ctgtgcctgc tcgggtggaa taatctcttc aaatttccca atccacatct cccgcagatc
     3721 cattgacggg aagatcagat gcatctcctt ctccccgtca cgggaagcaa ttgtcagccc
     3781 aatcccgggc tcacctaact ctcctgcaat gcggactgct tttttcagtg tatttaccgc
     3841 gaactctccc gcaaccgcaa gcatcccgtt tgtgttactc atcaggacaa accttgtgtt
     3901 tgtcagatat gcggtaaatg atgcacgttt cacccggatt cccggagaag agatgtatgt
     3961 cttctcccct ggctgca
//
LOCUS       DALTZZ010000215         2821 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_35371,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000215 DALTZZ010000000
VERSION     DALTZZ010000215.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35371"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(28..327)
                     /locus_tag="O0X12_04100"
     CDS             complement(28..327)
                     /locus_tag="O0X12_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HJK53370.1"
                     /translation="MNRATTEIILGIAVIVIFVIGTLFLIPSGGEDGEGWGGADGGAA
                     DLIDSTGYVPWFNPIWEPPSGEIESLFFCVQTAIGAVIVGYFFGYWRGAKGRKEE"
     gene            complement(324..1019)
                     /locus_tag="O0X12_04105"
     CDS             complement(324..1019)
                     /locus_tag="O0X12_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048061969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HJK53371.1"
                     /translation="MHIMEGYLPIEWCVLWAVLSAPCVIYGLWRMKQMIQQDRRVLPL
                     MAVCGAFVFVLSALKIPSVTGSCSHPTGTGLSASFFGPWVTAVLGTIVLLFQAILLAH
                     GGLTTLGANVFSMAIAGPFVAWLVFVALRRYAHAGLGIAVFVTAAVANLVTYSITSLQ
                     LALAFPADGSVFAAFVAFAAIFAVTQIPLAVIEGIICGLVAKYIVRVKPDILKKLGII
                     EDAEIAKIQEEAA"
     gene            complement(1230..2171)
                     /locus_tag="O0X12_04110"
     CDS             complement(1230..2171)
                     /locus_tag="O0X12_04110"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HJK53372.1"
                     /translation="MDKNLYVKDITNGSDVSSLFLIKRSDLRSKRGEQFVHMTLADRT
                     GTIECKAYGNKAVEAARAVKTGMVYAISGVGKINQNGELHVFSDSPETLDSLLAGPVT
                     VMPGQETEFLFTPADIKGNAAEIQKVVASIGNNDLRLFVACCLDHRFYECPAAIRRHH
                     EYLGGLCEHSLETVRIALNLVETMPRTEINRDMVIAGAVLHDIGKIFCFDKVGYGYVP
                     NDTYNLIEHITMGIMFIDEYKSLISESQLLQLRHIIQSHHGIYGDVVPLTAEAWLVNQ
                     ADGASSMLRSIVDDLAETPKGQKKKGLRSEKFLWKAV"
     gene            complement(2228..>2821)
                     /locus_tag="O0X12_04115"
     CDS             complement(2228..>2821)
                     /locus_tag="O0X12_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HJK53373.1"
                     /translation="VQAGYAGLLVSFDEVETAQAFARPQREKGYINLRQIVDMIDRNE
                     LPNCFFLFAGTPALFDSAKGIRSLPPLYDRIGMIADDGFSNPMQTQIVLPKFDVKKLE
                     EVSLRVIDIYTEAYSEVDKTRVSIRFIRTMIDRVTGRFGGRVDVVPRLYLREFVDVLD
                     KCALYDSYNPMEKYAFVAKENDTSLKEEEKAVMEVSW"
BASE COUNT          747 a          742 c          643 g          689 t
ORIGIN      
        1 cggcttttcg tccacgtgtg tactcactta ctcttctttg cggccttttg ctcccctcca
       61 gtatccaaag aagtacccga caatcactgc accgattgcg gtctgcacac agaagaacag
      121 tgattcaatc tccccgcttg gcggttccca aattgggttg aaccagggaa catacccggt
      181 gctgtcgatc agatcggcgg ctcctccgtc tgcaccgccc cagccttccc catcttctcc
      241 tccggaagga atcaggaaca gcgtcccaat cacaaagatc acaatcacgg caatgccgag
      301 gatgatctcc gtcgtcgccc ggttcatgct gcctcctcct gaatctttgc aatttcagca
      361 tcctcgatga tcccgagctt cttgagaatg tcaggtttga cacggacaat atactttgca
      421 acaagaccgc agatgatacc ctcaatgact gcaagcggaa tctgggttac ggcaaagatt
      481 gctgcgaatg caacaaatgc tgcaaaaaca cttccgtcag cagggaatgc aagggcgagc
      541 tgcagggaag ttatcgagta agttaccagg tttgcaactg ctgcagtgac aaacactgca
      601 atgccgaggc ctgcatgggc ataccggcgc agtgcgacaa agaccagcca ggcaacaaac
      661 ggacccgcga ttgccattga gaacacgttt gctccaagcg tggtaagacc gccgtgcgca
      721 agtaaaattg cctgaaataa caatacaatg gttccaagaa ctgcggtgac ccacggaccg
      781 aaaaatgaag ctgacagacc tgttccggtg ggatgcgaac agcttcctgt cactgacgga
      841 atcttgagtg cggaaagtac aaacacaaac gcaccgcaga ctgccatcag agggagaacg
      901 cgtctgtcct gctgtatcat ctgtttcatc ctccacaagc cgtagatgac acatggtgct
      961 gagagcacag cccacagcac acaccattcg atgggcaaat acccttccat aatgtgcata
     1021 aagtgtactt gttctaatta ttatttatat gttatgaaat caaattataa tttatattag
     1081 aaaaataatt tgtgttgtgt gatcttataa ggacgagcca ttcagtaccg atgcagcgca
     1141 taatgatgga gtaccagacc agtactgctg cggaaaaata gtttttatcg gaaaatggag
     1201 gccgtatgta cctgaaaaag aaaaggagat cagacagcct tccagaggaa cttctcgctc
     1261 cggagaccct tcttcttctg accctttggt gtctctgcca gatcatccac aatgctgcgg
     1321 agcatcgaac tcgctccgtc tgcctgattc accagccacg cctcagcagt caggggcaca
     1381 acatcaccgt agattccgtg atgtgactgg ataatatggc ggagctgtaa aagctgtgac
     1441 tcggagatga gactcttgta ctcatcaata aacataattc ccatcgtaat atgctcgata
     1501 aggttgtagg tatcgttggg cacatagccg taaccgactt tgtcaaagca gaagatcttt
     1561 ccaatatcat gcagcaccgc accggcaatc accatgtcac ggttgatctc ggttctgggc
     1621 attgtctcca caagattcag cgctattcgt accgtttcaa gggagtgttc acaaaggccg
     1681 ccgaggtatt catggtgacg gcggattgct gccgggcatt cataaaaccg gtgatccaga
     1741 caacaggcaa caaacagcct gagatcattg tttccaatgc ttgcaacaac tttctgaatc
     1801 tcagctgcat tccctttgat atccgcaggc gtgaacagga actcggtctc ttgccctggc
     1861 atcacggtaa caggtcctgc aagaagtgaa tccagcgtct ctggagagtc cgaaaagaca
     1921 tgcagctctc cgttctgatt gatcttgccg acacctgata ttgcatacac cattccggtc
     1981 ttcaccgccc gtgcagcctc cactgcttta ttgccgtagg ctttgcactc aattgttccg
     2041 gtacgatctg caagagtcat gtgaacaaac tgttcaccgc gtttactgcg gagatctgaa
     2101 cgtttgatga ggaaaagaga cgatacgtct gatccgttcg taatatcttt tacatagaga
     2161 tttttatcca tggatacttc cgtggatact tataggcagt gaaaaaataa aggattgtag
     2221 gaatctctca ccagctgact tccatgacag ctttttcctc ctcttttaac gaggtgtcgt
     2281 tctcttttgc aacaaatgca tacttctcca tcggattata ggagtcatac agtgcacatt
     2341 tgtccaacac atccacaaac tcccgcaggt acaagcgcgg caccacatcc acgcggccgc
     2401 caaatcttcc tgttacccga tcgatcattg tgcggataaa ccggatggag acacgcgttt
     2461 tgtcaacctc ggagtatgct tcggtgtaga tgtcaatcac ccgcagagaa acctcttcaa
     2521 gttttttcac gtcaaacttc ggaagaacaa tctgtgtctg catgggattg gagaatccgt
     2581 catcggcaat cattcctatt cgatcataca gaggaggaag tgatcgaata ccttttgcac
     2641 tgtcaaagag tgcgggcgtt ccggcaaaca ggaagaaaca gttcggcagc tcatttcggt
     2701 cgatcatatc cacgatctga cggagattga tgtatccctt ctcccgttgc ggtctggcaa
     2761 atgcctgagc agtctccacc tcatcgaaac tgacaagcag ccccgcatat ccggcctgca
     2821 c
//
LOCUS       DALTZZ010000216         2196 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_35440,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000216 DALTZZ010000000
VERSION     DALTZZ010000216.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2196)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2196)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2196
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35440"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            40..318
                     /locus_tag="O0X12_04120"
     CDS             40..318
                     /locus_tag="O0X12_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HJK53374.1"
                     /translation="MSEITILAFASFREKFGEKNTVTISNGATILSVLHSFAESVPAA
                     RDELFDGDSLRGHVILMYNRERIDADDAKEIAVSDGDEIVLYPPVSGG"
     gene            322..723
                     /locus_tag="O0X12_04125"
     CDS             322..723
                     /locus_tag="O0X12_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HJK53375.1"
                     /translation="MVLSVQKEDIDIGALITASRTHADGAQLVFVGCVRDDGMDELEI
                     EAFVPVAEKDLADIAAEAKELFHLNSVDIVHRYGRLVLGETIVVIIVGASHRPEAYDG
                     SRYIIERLKERVPIWKQEISDGKKGAWVHGA"
     gene            705..1649
                     /locus_tag="O0X12_04130"
     CDS             705..1649
                     /locus_tag="O0X12_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HJK53376.1"
                     /translation="MGTRCMIDLPQSVRVAVCQLAPILFDKASTVQKTIEAIDEAARH
                     GAELVVFPESFIPAYPRGLSFGYVVGSRSMEGRYDWKRYYDNSMIVPSPDTDLIGSAA
                     AKAHVYVSIGVTERDATSATLYCTNLFFGPDGTLLGKHRKLKPTGTERCIWGEGDGST
                     LPIIRTPFGVMGSLICWENYMPLARAAMYQKGVSLYLAPTADSRDEWQATMRHIALEG
                     RCFVIGCNQYVTKSMYPPDLYCGGELKDLPENMCPGGSCIYDPFGRCVAGPVFGREEI
                     LYADLALDDIVLSRMDFDPSGHYSRPDVFVLKVCEPDR"
     gene            1839..>2196
                     /locus_tag="O0X12_04135"
     CDS             1839..>2196
                     /locus_tag="O0X12_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HJK53377.1"
                     /translation="MLVYVNGKFVPEEQATVSIFDHGFLYGDGVFEGIRAYNGRVFRL
                     MEHVDRLFDSAKAIDLIPPVTKDEMAEIIKETLRRNNLKNAYIRPIISRGIGSMGLDP
                     HHCEKPTVICAASEWGA"
BASE COUNT          511 a          496 c          576 g          613 t
ORIGIN      
        1 acgctgtcct gtctgttcca atatctgcga ggataaacca tgagtgaaat aacaattctt
       61 gcctttgcca gcttccgtga aaaattcggt gaaaaaaata ctgttaccat ctccaacggt
      121 gcaacaattc tgtcagttct tcacagcttt gcagagtctg ttccggctgc ccgtgatgaa
      181 ctgtttgatg gtgacagcct tcgcggacat gtcattctga tgtacaaccg tgaaagaatc
      241 gatgccgatg atgcaaaaga aattgccgtc tctgacggtg acgagatcgt gctgtacccg
      301 ccggtctcag gaggataaac aatggtcctg tctgttcaaa aagaggatat tgatattggc
      361 gccctgatta ctgcatctcg aacacatgcc gatggtgcac aactggtgtt tgtcggctgt
      421 gttcgtgatg atggcatgga cgaacttgaa attgaagcgt ttgtaccggt ggcggaaaag
      481 gatcttgccg acattgctgc agaggcaaag gaactgtttc acctgaactc tgtggatatt
      541 gtgcaccggt atggtcgtct tgtacttggt gagacgatcg tggtgattat tgtgggtgcg
      601 agtcaccggc ctgaggccta tgatgggtca cggtacatca ttgagcggct gaaggaacgg
      661 gttcctatct ggaaacagga aatctctgac ggaaaaaaag gtgcatgggt acacggtgca
      721 tgatcgatct tccgcaatct gttcgtgttg ctgtctgcca gcttgcccct attctgtttg
      781 acaaagcatc aacagtacag aaaaccattg aggccataga tgaggcagcc cgtcatggtg
      841 cggagcttgt ggtgtttcct gagtctttca ttcctgcata tccgcgcggt ctctcgtttg
      901 gatacgttgt cggtagccga agtatggagg gcagatatga ctggaaacgc tactatgaca
      961 actccatgat tgtgccaagt cctgatacgg atcttatcgg cagtgccgct gccaaagcac
     1021 atgtctatgt ttccatcggt gtgactgaac gcgatgctac ttctgcaacc ctctactgta
     1081 caaatctctt ttttggtccg gatggtactc ttttggggaa acatcggaag ctgaaaccaa
     1141 ccggaactga acggtgcatc tggggggaag gggacggaag cactcttccg atcattcgca
     1201 cgccgtttgg cgtgatgggt tctcttatct gctgggaaaa ttacatgccg cttgcgcgtg
     1261 ctgcgatgta ccagaaagga gtttctcttt atcttgcacc tactgctgat tcgcgcgatg
     1321 aatggcaggc aaccatgcgc cacatagcct tggaaggcag atgctttgtg attggttgca
     1381 atcagtacgt gaccaaatcg atgtatcctc ccgatctgta ctgtggaggt gaattgaagg
     1441 atctgccgga gaacatgtgt cccggaggta gctgtatcta cgatccgttc ggccgctgcg
     1501 ttgcaggtcc tgtttttggc cgtgaagaaa ttctgtatgc agatcttgcg ctcgatgaca
     1561 tcgttttatc ccgtatggat tttgatccct ctggacatta tagccgtcct gatgtttttg
     1621 tgctgaaagt ctgtgaacct gatcgataga tttcacgtac ctggctgtat tttccagtga
     1681 atggagtatg ggcactgact ctcatatttt tcagccgcga gttctcggga cttgggagta
     1741 atgtacgggt cttaattgtg tgtttctatg cgtttttcct atctcttccg atagtgagcc
     1801 gcacattaga tatagcaatc ccgacaaaca actcaatcat gttagtgtat gtcaatggaa
     1861 aatttgtccc tgaagagcag gcgactgtat ccatttttga tcacggtttt ctctacggcg
     1921 acggtgtgtt tgagggaatt cgtgcataca acggccgtgt gttccgttta atggaacatg
     1981 tggaccggct ctttgactca gcaaaagcaa ttgatcttat tccgccggtt accaaagatg
     2041 agatggccga gatcatcaaa gagactctgc gccggaacaa cctgaaaaat gcttacatcc
     2101 gtcctatcat cagccgtggt atcggatcaa tgggcctcga ccctcaccac tgtgaaaaac
     2161 cgacggtcat ctgtgcagca tctgaatggg gtgcga
//
LOCUS       DALTZZ010000217         2205 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_35443,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000217 DALTZZ010000000
VERSION     DALTZZ010000217.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35443"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            40..498
                     /locus_tag="O0X12_04140"
     CDS             40..498
                     /locus_tag="O0X12_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HJK53378.1"
                     /translation="MQQITIHLGATGITPTSTFSGGNISPKIDLTGVPAEVKYIAVIV
                     QNKSGEGKTHCIWSVWNIPSVGYIPPGYEQGPAPSFPFPAVQGLNDFGEHGWHGPESK
                     LGSQERMLFQVFGRAEPLSISADAKMDEVIEALRDGKTTAYGSLESTYFL"
     gene            1023..1427
                     /locus_tag="O0X12_04145"
     CDS             1023..1427
                     /locus_tag="O0X12_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJK53379.1"
                     /translation="MVLITEEIKEQIAKVGVCHLITASKAGVPNAAPMGGLWVMDNDT
                     IWISNNFMNKTAANVRENPQAALLVWSRELGNCIQLKGTATLESGTPEHAKMMEMMEA
                     KKPGLPKKELLKLVVKEIYTCMPGPAAGSKIA"
     gene            complement(1434..>2205)
                     /locus_tag="O0X12_04150"
     CDS             complement(1434..>2205)
                     /locus_tag="O0X12_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CCA-adding protein"
                     /protein_id="HJK53380.1"
                     /translation="AKTVRKKFSEPLVVIDPTDRDRNVAAALTPTRFAEFMELARDYC
                     AAPSPVYFLADPPTQMGRGEFAEVLEKRGTAMLAIRMKTPPYVADTVVPQLRKSMESI
                     RSMLVAEDFQIHRAEVSMGGEHSLIIFELLVDRLPEIVVRAGPPVWNHENADRFVDKY
                     LTKGEFAGPWIEEDRYYTEVRRKYTTARSLLEDAPKILSGALGKHVRQVMEESGYEVL
                     SGTDIWSAEFSFFLSAFFARSSHAIRKLREIGQTPAQK"
BASE COUNT          563 a          561 c          605 g          476 t
ORIGIN      
        1 aatctataca tctattaggt tctcgggtga gtgtactgta tgcagcagat aaccattcat
       61 ctcggggcga ccgggattac gccgacaagt acgttctccg gcggaaacat ctcgccaaag
      121 atcgatctga ccggcgttcc cgccgaggta aagtacatcg cagtgattgt tcagaacaag
      181 tccggagaag gaaagacaca ctgtatctgg tcggtctgga atattccttc ggtagggtac
      241 attccgccgg ggtatgagca gggccctgcg ccttccttcc ccttccctgc ggtccagggc
      301 ctcaatgact tcggcgagca cggctggcac ggaccggagt caaagcttgg cagtcaggag
      361 cggatgctgt ttcaggtctt cggccgcgcc gagccgttgt caatctctgc ggatgcaaaa
      421 atggatgagg taattgaggc tctccgtgac ggaaaaacca ctgcctacgg aagtcttgag
      481 tcaacctact tcctctaaac cccgttcttt tttgcggcgt gccgcagttt gaagcaaaaa
      541 aaatcctgat agatcgccgc ggttgtaccg gccttgtgcg cgaggagaaa tccaagtgtt
      601 ttacaggact aatttatcgc tagatttcaa ggtattttca aaactggcag aaaactgaga
      661 tttttcatta ccctgacaca cagctttata caccccaaaa gcagatgaaa atgtatgtca
      721 cacaaagcgg caaaaaagac agcagcaccg gttgcagaag aaaaagtcga agcaacgaag
      781 gcagctggaa agcactccaa gaagaactaa caacttcttt ggaacagttt gatgaactga
      841 tgagtttgta aaaaaatgtg tgacaccccc tgacatctga taaagatgtc tgagcctgcc
      901 acaggcaccc ggcctgtgtt cccggagttt ttccggaaaa cacaggccga tacaaagagg
      961 ctctttttct gggttgtgta ttttccgcaa agttgacatg caaagaaggc acagctacac
     1021 acatggtatt gattactgaa gaaataaaag agcagattgc aaaagtcggt gtctgccacc
     1081 tgattaccgc atcaaaggcc ggtgttccca atgcagcacc gatgggcggc ctgtgggtca
     1141 tggacaatga caccatctgg atctcaaaca acttcatgaa caagaccgca gcaaacgtac
     1201 gggagaatcc gcaggcggca ctccttgtct ggagccgcga gctgggtaac tgtatccagc
     1261 tgaagggaac ggcaacgctt gagtccggaa ccccggagca tgcaaagatg atggagatga
     1321 tggaggcaaa aaagccggga cttccgaaga aggaactgct gaaacttgtg gtaaaagaga
     1381 tctacacctg catgccggga ccggcagcag gatcaaagat cgcataacta tttttatttt
     1441 tgagcgggcg tctgtccgat ttcgcgcagt tttcgaatcg catgcgagga gcgggcgaag
     1501 aatgcagaga ggaagaagga gaactccgcc gaccagatgt cagtgccgga gaggacctca
     1561 tagccggact cctccatcac ctgccggaca tgtttgccca gagcgccgga gagaatcttt
     1621 ggtgcgtcct caagcaggga cctggcagtt gtgtacttgc ggcggacctc ggtgtagtag
     1681 cggtcttcct caatccaggg acctgcaaac tcgccttttg tcaggtattt gtccacaaac
     1741 cggtcggcgt tttcgtggtt ccagaccgga ggtcctgcac ggactactat ctccggcagc
     1801 cggtcgacaa gcagttcaaa gatgataagc gagtgttcgc ctcccattga gacctcggca
     1861 cggtgtatct ggaagtcctc ggcaacaagc atggagcgaa tcgactccat actcttgcgc
     1921 agctggggta caacggtgtc agcgacatat ggcggggttt tcatccggat ggcaagcatt
     1981 gcggttccgc gtttttccag aacctcggca aactcacccc ggcccatctg tgtcggcggg
     2041 tcggcaagga agtacaccgg ggacggggct gcacagtagt cccgcgcaag ctccataaac
     2101 tcggcaaacc gtgtcggtgt aagggcggca gcaacattcc ggtcccgatc ggtcgggtca
     2161 atcaccacca gcggttcgga gaactttttc cggacggttt ttgca
//
LOCUS       DALTZZ010000218         2353 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_35484,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000218 DALTZZ010000000
VERSION     DALTZZ010000218.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35484"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="O0X12_04155"
     CDS             complement(<1..1043)
                     /locus_tag="O0X12_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HJK53381.1"
                     /translation="MVGRHYDCIIIGAGPAGLFCASLLAPARVLVLEKMVLPGRKLLI
                     AGSGQCNITHTGDVKNFVSHYGEHGAFLRPALMAFPNTAVREFFERLGVSFVEKESGK
                     VFPESHSADDILDALLDACDAAGVEILSYTPAESVACENGMYAVKTLHTTYYAKILVI
                     ATGGMSYPQTGSTGDGFLFAKALGHSVTPPCPALTPVYAVRHQLADLSGISVSGATIS
                     IWRGGKKLLVRSGDLLITRFGYSGPVILDSARWMRTGDILRIGFCKKKPEELDTLIRA
                     ACSSAGTKQIQNLLDDAECPDRLVKHMVQESGIPEGTTGAQLTASQRSRLVENLTSYP
                     VEIDHLGDFRVAMA"
     gene            1343..1801
                     /locus_tag="O0X12_04160"
     CDS             1343..1801
                     /locus_tag="O0X12_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HJK53382.1"
                     /translation="MARIHARRRGIASSVRPFRQEVPEWSNTDVQEIEKKIIELRKAG
                     LSCAEIGLVLRDKFGVPNVKLVTGKRVNAIVRENDLDTDIPEDLRNLMHKALGMRKHL
                     GENKKDLHNKRQLQLTESKVRRLVKYYVGTGRLPQGWTYKPETAEILLSR"
     gene            1846..>2353
                     /locus_tag="O0X12_04165"
     CDS             1846..>2353
                     /locus_tag="O0X12_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HJK53383.1"
                     /translation="MAKHLASLEYVEIYSHYDADGIAAAAIMSLALSRADIAFKLRIL
                     PGISEDDVENPDISLLCDFGASCAGLEESTMIIDHHVPYNTSPYHVNPRLFGENGESE
                     LSGAGCAYVVANALGDNRDLAGLVMLGIIGDNQIISGRNQMIVGDAIANNLINPGRGI
                     LLSGRTTTE"
BASE COUNT          580 a          622 c          558 g          593 t
ORIGIN      
        1 gccattgcca cccggaaatc tcccaggtga tcaatctcta caggataaga cgtgagattt
       61 tctaccagtc tgctccgttg tgatgccgtg agctgggctc ctgttgttcc ctcaggaatt
      121 cctgattcct gtaccatgtg tttcaccaac cggtccgggc attcagcatc atccagcaaa
      181 ttctgtattt gtttcgttcc cgcagaagaa cacgcggcac ggatcagtgt atccagctct
      241 tccggtttct ttttacaaaa tccaatcctt agtatgtctc ctgttcgcat ccatcgggca
      301 gaatccagaa tcaccggtcc ggaataacca aatctggtga tcagcagatc tccggaacgc
      361 acaagcagtt tctttccccc gcgccagatt gagatcgttg ctccggatac agaaatcccg
      421 gacaaatcag ctaactggtg ccgtaccgca tagaccggtg ttagtgccgg gcagggtggt
      481 gtaacggagt ggccaagtgc tttcgcaaac aaaaatccgt ctcctgttga tcctgtctgt
      541 ggatatgaca tacctcctgt tgcaatgaca agtatttttg cataatatgt cgtatgcagc
      601 gtttttactg catacattcc gttttcacag gcaacgcttt ctgcaggcgt gtaactcaga
      661 atctcaacgc ctgctgcatc gcaggcatcc agcaatgcgt ctaaaatatc gtctgcggag
      721 tgtgattccg gaaacacctt tcccgattcc ttttccacga atgaaactcc taatctctca
      781 aagaattctc gcactgccgt gttggggaat gccattaatg caggccgcaa aaatgcaccg
      841 tgttctccat aatgggaaac aaaatttttc acgtccccgg tatgcgtaat attgcactgg
      901 cctgaccctg caatcagcag ttttcgtccc ggaagtacca tcttttccaa gaccaatacc
      961 ctcgcaggag caagaagtga tgcacagaaa agacctgctg gtcctgcccc gataatgata
     1021 cagtcatagt gccggcctac catacccagt atattttccc gcaaccctta tctcatcttc
     1081 tctcctatat agaagaagca ctttacagtg ctggctgaag taagtccgac gtgtctgtct
     1141 gttacgacac tgccattctt ggctttggga ttacgatgag tcagttcggc tccgcagaat
     1201 gtctgcggga atgcttgttt gcaacagatg tacttgttgc agctgctctt ttcgtgactt
     1261 acggctgttt ctctttgatt ccgcaaagga agcggttatc cgcctgtacg gggcggacag
     1321 tccttaatcg gaggtgaaaa caatggcacg catacatgca agaagaagag gaatcgcctc
     1381 ctcggtcaga ccattccgtc aggaagttcc tgagtggtcc aacaccgatg tacaggagat
     1441 tgaaaagaag atcatcgagc tccgcaaagc aggtctttcc tgtgcagaga tcggtctggt
     1501 tctgcgggac aaatttggcg ttccgaacgt aaaacttgtt accggcaaga gagtcaatgc
     1561 catcgtccgt gaaaacgacc ttgacaccga catccctgaa gatctgcgca acctgatgca
     1621 caaggcactt ggcatgcgca agcaccttgg cgagaacaag aaggatcttc acaacaagcg
     1681 tcagctccag ctgaccgagt cgaaagtccg cagactggtg aagtactacg tcggtaccgg
     1741 ccgcctgccc cagggctgga cctacaagcc ggagaccgca gagattctcc tgtccagata
     1801 agatttccat tccgccgtat gtcactagaa accgcagcag aatccttggc aaaacatctc
     1861 gcatcacttg agtatgtgga aatctactca cattacgatg cggatggcat tgcagctgct
     1921 gcgatcatgt cactcgccct gtccagggca gatatcgcct tcaaactccg gatccttccg
     1981 gggatatctg aagatgacgt ggagaacccg gacatatctc ttctgtgtga cttcggtgcg
     2041 tcgtgtgccg gtcttgaaga gagcacgatg attatcgatc atcatgttcc ctacaacacc
     2101 tctccctatc atgtcaaccc gcgcctcttt ggtgagaacg gtgaatccga actttccgga
     2161 gcagggtgtg catatgtggt ggcaaatgcc cttggagaca accgtgatct ggcaggtctt
     2221 gtaatgcttg ggatcattgg tgacaatcag atcatttccg gaaggaatca gatgattgtc
     2281 ggtgatgcaa tagcaaataa tctgataaat ccgggtcgcg gcattctcct ttcgggcaga
     2341 acaacaacag aac
//
LOCUS       DALTZZ010000219         9001 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_35700,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000219 DALTZZ010000000
VERSION     DALTZZ010000219.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35700"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="O0X12_04170"
     CDS             <1..416
                     /locus_tag="O0X12_04170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJK53384.1"
                     /translation="IRCREIGRRPSHAPVEEKSLIYECCGGKEHFLSTVSGEALIGFV
                     RLRFPGTVFRSELDGAALVRELHVYGSIVPLGERGEGEDRQHRSYGQKLLSRAEETAR
                     DAGYDKVAVMSGVGVRPYYRKQGYVRTGPYMIKNL"
     gene            413..1558
                     /gene="priS"
                     /locus_tag="O0X12_04175"
     CDS             413..1558
                     /gene="priS"
                     /locus_tag="O0X12_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase catalytic subunit PriS"
                     /protein_id="HJK53385.1"
                     /translation="MKPATYEFLRQRFSAYYNGEVQGAGAVYIPEALTEREWGFIFFS
                     SGSKTAGMRRHLSFSSPEDVHTYLKTMIPAHVYYSTAYYARPGAGQMSDKGWLGADLI
                     FDLDADHIVRGPYDVMLARVKEELFKLIDMLTSELGFFERDLRINFSGGRGYHIHIPT
                     LAVRSWDSTERRELVNYVSGTGLSAESMLTGPARNKGWQKRYRAALLAELDRIAALES
                     DAQAEYLTGLKGISDKFATGFLKNLDATRTTAATIPEKLLENKVIRAITNPENKPFQD
                     QILAMAAQADEPVTTDIKRLIRHPGSLHGGSGMRVTPLEVDRLDAFDPLLDAVVFGER
                     QVTVECSFPLTMPILGNSYSLSKGLNTVPEALAVFLCCRGIAEIGGV"
     gene            1560..2261
                     /locus_tag="O0X12_04180"
     CDS             1560..2261
                     /locus_tag="O0X12_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication complex GINS family protein"
                     /protein_id="HJK53386.1"
                     /translation="MPDRISISELHGYLIDERNSGSLTEIPATLYEEVHAELNALTSE
                     ARSMGDPFGEGVQILLKERESLREYIRDLYAERTRKIFSLALAKATGEEINREELRCM
                     VPGERSLYEVVADSATACRKALLDGKQILDTTTAFHFSPGEAGIVADEPNSTDAASFA
                     DAHADEVFQNGNADAETAPESYRVIMVQEKVEEFQDYSGRCYALSPGDIVSLPQPMAE
                     ILCNRNIALNIRLRK"
     gene            2303..2581
                     /locus_tag="O0X12_04185"
     CDS             2303..2581
                     /locus_tag="O0X12_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HJK53387.1"
                     /translation="MKKPVKFNTYCPYCRKHTEHEVEKVKKGKTTGLHWIDRQKARRS
                     SVGNRGKFGKVPGGDKPTKKINVRYRCKECGKAHLRAGYRAGKFELTE"
     gene            2588..2776
                     /locus_tag="O0X12_04190"
     CDS             2588..2776
                     /locus_tag="O0X12_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HJK53388.1"
                     /translation="MVKSTRENRSKFLKVKCPDCEHEQLVFEKASSKVECIVCGRVLA
                     EPTGGKADIKADVIASYE"
     gene            2794..3567
                     /locus_tag="O0X12_04195"
     CDS             2794..3567
                     /locus_tag="O0X12_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HJK53389.1"
                     /translation="MSERDWPIEGELVVCSVVEVKDFAAFVALDEYEGRQGLIPIAEI
                     ARGWIKYIRDYIREGQKVVCKVLHVDESRGHIDLSLKDVNEHQRREKIQDWKNEQKAH
                     KWIEFASKDSNVPVKDIEEAMYKEYSSLYAAFEDIAIYGDTTLEKFSLPEVVRNALAK
                     VASENVKVPRVTVSAILELTSNKPDGVNVIRRALRSAEPKVDGAEIELLYLGAPKYRV
                     KVVAPDYKTAEKALEKAANAAIGVMERADGTGKLVRKQK"
     gene            3579..3740
                     /locus_tag="O0X12_04200"
     CDS             3579..3740
                     /locus_tag="O0X12_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HJK53390.1"
                     /translation="MTGHIRQCPIDHTYTLLKICPKCGAATESVHPARYSPQDKYGKY
                     RRMIREWNK"
     gene            3728..4486
                     /locus_tag="O0X12_04205"
     CDS             3728..4486
                     /locus_tag="O0X12_04205"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HJK53391.1"
                     /translation="MEQVNVRWYVEDVSAHSASVVIAGLPGVGHVGKLVVDHLVRALS
                     TVKVAEITSTLFPPQMYLDCDAVLRLPRNEIFFVPGTDEHDPVLFLAGDCQSTSAEGH
                     YTLAEGYLRVFDLLGVRRIYTLGGYGVGRMVEHPRVLSALSSASLKEEVLAAGAVINP
                     EEPVGGIIGAAGLLITLGRLHGMEGVALLGETSGYLVDPVSSTAVLDVLERLTHIHAD
                     RTELTDLAEQMMTDIAAIANAATPKPPDDLSYIG"
     gene            4492..5610
                     /locus_tag="O0X12_04210"
     CDS             4492..5610
                     /locus_tag="O0X12_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HJK53392.1"
                     /translation="MQKNTDLHLHSCFSMATSPDMVPEHILAGCRIKGIDVIGSGDAL
                     HPEWRKMWEPYVDNEFGITVIPQTEVEDSSRVHHVILAETFDQFAELQDMFEPVCGHL
                     TTAGRPHLQLGGEEIATAVHDVGGMIGPAHAFTPWTSLFAAFTSPSECYGEETFEFCE
                     LGLSADSTYGAGIAEFADVPFLTNSDAHSPTPIKLGREFTRMDLSTDSPKSVLAGISS
                     GNIILNAGFFPEEGKYNRTACTRCYQQFSLAEAEHFHWKCPHDNGRIKLGVRERAEKL
                     STLAEPTQRPPYLKMIPLGEMIARVLGTASPNTKKCKELYNRFVETFDNEIAVLLEIP
                     KQDLCSVSCEVADAVIKMREGQVTLFPGGGGKYGSFAF"
     gene            complement(5651..6481)
                     /locus_tag="O0X12_04215"
     CDS             complement(5651..6481)
                     /locus_tag="O0X12_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HJK53393.1"
                     /translation="MSSLGIIHHCDCITGMNRLSAGSVDVIVTSPPYNLGKLYTTYDD
                     TVPRNGYLSWMKDVADAAIRVLSDEGSLYLNVGGSLKDPWIPMDVAQEFRRSGFILQN
                     MIHWIKSIALPKEDMGKFYNAKIPENGVAVGHYKPINSHRFHNDCHEFIFHFTKTGDV
                     PIDRLANGVPYQDKSNVRRWVQKKQDIRDRGNTWFIPYETVREERPHPATFPVQLPMM
                     CIRDHGVSRCRLVMDPFMGIGSTAVAAVRLGIPFIGFEIDEGYINIAEERIAAELLPQ
                     "
     gene            complement(6484..6909)
                     /locus_tag="O0X12_04220"
     CDS             complement(6484..6909)
                     /locus_tag="O0X12_04220"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HJK53394.1"
                     /translation="MSGVTAESTLLLHFTSRRPDGEIFEDTRSGKPVLVTLGKKQINP
                     AFEEALIGKSEGETVTVVLPPEKAYGRYHKKLVVTIKRHKLSLDHEPVAGEIIRVEVL
                     KKPCLVTVVGVTDKSITVDANHPLAGETITYDITIEKIF"
     gene            complement(6970..7920)
                     /locus_tag="O0X12_04225"
     CDS             complement(6970..7920)
                     /locus_tag="O0X12_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HJK53395.1"
                     /translation="MEIAYAVLVIVGLVAFETVASIDNAIINADILSTMKAWARRWFL
                     TWGIIIAVFGIRGVLPWLIIWFSTPSLGPIGALTATFSDDATAAAAIEASAPFLLLAG
                     GIFMIFLFLHWLMAEEKNCIVPGERAMSRQGPWFYAVAAVVLFLICYAAAQINALLAV
                     AAVGGSAIFFIMQGFRLFADKKSAELEAGTSTKSDMSKLMLLEVIDATFSIDGVIGAF
                     AFTMSVPLILIGNGIGAVVVRELTVRNIENIRRYAYLKNGAMYSIFTLGLIMCGEGFG
                     LHIPIWFAPLLTIGIVATFFWLSLRRIKKGDFGVCHIPRE"
     gene            8116..8247
                     /locus_tag="O0X12_04230"
     CDS             8116..8247
                     /locus_tag="O0X12_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53396.1"
                     /translation="MSVCAEKEMERTTHSEPAGATMTVSERQGGEMEWQSPRTELEE"
     gene            8255..8857
                     /locus_tag="O0X12_04235"
     CDS             8255..8857
                     /locus_tag="O0X12_04235"
                     /inference="COORDINATES: protein motif:HMM:NF021414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 family protein"
                     /protein_id="HJK53397.1"
                     /translation="MNVLSLSDDESAAVIDRTIQSLQKATSTADAVKRIGSALSQYTV
                     FDLQYIGGFIRREVNKLPNPYRRVYLPYSLDLLNQYHVFMQMYRTQKLPDEPIKDPTL
                     WSEYWEIARVQCFVRSGPSDDPFPQMEHPLSKFFYRLVYGYVMLIANGYGHPIGMPFP
                     GGATVHREGEQVLCPIRDKERDLPAALCNFCPAEQDPRYK"
BASE COUNT         2489 a         2153 c         2326 g         2033 t
ORIGIN      
        1 gcatcagatg ccgtgagatt ggacgaagac ctagccacgc accagttgaa gaaaaatccc
       61 tgatctatga atgttgcggc gggaaagaac actttctctc cacggtttcc ggagaagccc
      121 tcatcggatt tgtacggttg aggttccccg gcactgtctt ccgctcagag cttgatggag
      181 cagcactcgt tcgtgaactg catgtatacg gcagcattgt tccccttggg gagcgcggtg
      241 agggggagga cagacaacat cgatcctacg gccaaaaact gttgtcccgc gcagaagaga
      301 ctgcgagaga tgcgggatat gataaagtcg cggttatgag cggtgtaggg gtacgaccct
      361 actatcgcaa acagggatat gtgcggacag gcccatatat gatcaagaac ttatgaagcc
      421 agccacctat gagttcctca ggcagcggtt ctctgcctac tataatggag aggttcaggg
      481 agcaggagct gtgtacatcc ctgaggcact tactgaacgg gaatggggct tcatcttctt
      541 ttcaagcgga tcaaaaacag ccgggatgcg caggcatctt tcctttagtt caccagagga
      601 tgtacacacc tatctcaaaa ccatgatccc cgcgcatgtt tactactcaa ccgcgtacta
      661 cgcgcggcct ggagcggggc agatgagtga taaaggatgg ctgggagcag atcttatttt
      721 tgaccttgat gcggaccaca tagtcagagg accgtatgat gtcatgcttg cgcgggtcaa
      781 agaggagcta ttcaagctca tcgacatgct gacatcggaa ctcgggtttt tcgagcgcga
      841 cctcaggatc aatttctccg gtggccgcgg ttatcatatc cacataccaa cccttgcagt
      901 ccgcagttgg gacagtactg agcggcgaga actggtcaac tacgtctcag gaacaggtct
      961 ttccgcagaa agcatgctta caggtcctgc gagaaacaaa ggctggcaaa aacgttaccg
     1021 tgcagcactt ctcgcagagc ttgacagaat agccgcactg gagtctgatg cacaggcaga
     1081 ataccttacc ggactcaaag ggatctccga caagtttgcg acagggtttc tgaaaaatct
     1141 tgatgcaacc cgtacaaccg ctgcaacaat cccggaaaaa cttctcgaaa acaaagtcat
     1201 ccgggcgatt acaaatccag aaaacaaacc atttcaggat cagatcctag caatggcggc
     1261 tcaggcagat gaaccggtta cgactgacat taagcgtctc atccgccacc ccggatctct
     1321 tcacggcggc tccggcatgc gggtaactcc ccttgaggtt gaccgtcttg atgcatttga
     1381 tccgcttttg gacgccgtgg tctttggcga gcgacaggta acagttgagt gttccttccc
     1441 actgacaatg ccgatacttg gaaacagcta cagtctttcc aaaggattaa acacagtccc
     1501 cgaggcactt gcagtattcc tctgctgccg cggaattgca gagatcggag gagtgtaaca
     1561 tgccggatcg catcagcatc tcagaacttc acgggtacct gatcgatgaa cgcaacagcg
     1621 gatcgctcac cgagatacct gcaaccctgt atgaagaggt gcatgcggaa ctcaatgcat
     1681 taaccagtga agcgcgcagc atgggagatc cattcggaga aggagtacag attctcttaa
     1741 aagaacgtga gagcctgcgc gagtacatcc gggatctcta tgccgagcgg acccgcaaaa
     1801 tcttttcact ggccctcgcc aaagcaaccg gggaagagat taaccgcgaa gagctccgct
     1861 gcatggtacc tggagaacgt tcactctatg aggttgtcgc agactcagct acggcatgca
     1921 gaaaggctct tcttgacggt aaacaaatat tagataccac aaccgcattc cacttcagcc
     1981 ctggagaggc agggatagtg gcagatgagc cgaattcgac tgatgcagcc tcttttgcgg
     2041 acgcgcatgc agatgaagtg ttccagaacg gaaacgccga tgcagaaact gctcccgaat
     2101 cgtaccgcgt gattatggta caagaaaaag tagaggaatt tcaggattat agcggacgat
     2161 gttatgctct ttcgccagga gatatcgttt cgctgccaca acccatggca gaaattctct
     2221 gcaaccgcaa catagcatta aatatcaggc ttcgcaaata agtatggtat tcggtatcct
     2281 tgatttagag gggacaatat tcatgaaaaa accagtaaaa ttcaacacct actgcccgta
     2341 ctgccggaag cacaccgagc atgaggtgga aaaggtaaag aagggtaaga caaccggcct
     2401 ccactggatt gaccgtcaga aggcacgccg cagctctgtt ggaaaccgcg gtaagttcgg
     2461 taaggttccg ggaggagaca aaccgaccaa gaagatcaac gtacgttacc ggtgcaagga
     2521 gtgcggaaag gcacacctga gagcaggata ccgcgctggc aaatttgaac ttacggagtg
     2581 agcaagaatg gtaaaatcaa cccgcgaaaa ccggagcaag tttctgaagg ttaaatgccc
     2641 agactgcgag cacgagcagc tcgtctttga gaaggcatcc tctaaagttg agtgcatcgt
     2701 ctgcggacgt gtccttgcag agccgaccgg cggtaaggca gacatcaaag cagatgtcat
     2761 cgcatcttac gaataaattc ggaatcatca tcaatgagtg aaagagactg gccaattgaa
     2821 ggagaacttg tcgtctgcag tgttgtagag gtcaaggact ttgcggcatt tgttgccctg
     2881 gatgagtatg aaggacgtca ggggttaatc ccgattgcgg agattgcccg tggatggatc
     2941 aagtacatcc gggactatat tcgtgaagga caaaaagtcg tatgcaaagt ccttcatgtg
     3001 gacgaatccc gcggccatat tgatctttcc ctcaaagatg taaatgaaca ccagcgccgt
     3061 gaaaagattc aagactggaa gaacgaacaa aaagcccaca aatggataga gtttgcctca
     3121 aaggacagta atgtcccagt gaaggatatt gaagaggcaa tgtacaagga gtattcctct
     3181 ctctatgctg catttgagga catcgccatc tatggtgaca caacactgga aaagttctcc
     3241 ctgcctgagg tcgtaaggaa cgcactggca aaagttgcat cagaaaatgt gaaagttccc
     3301 cgggttacgg tttccgcaat cttggaactg acctccaata aaccggacgg agtaaacgta
     3361 attcgacgcg cacttcgttc cgctgagccc aaagttgacg gtgctgagat cgagttgttg
     3421 tatctgggcg cacccaaata ccgggtaaag gtcgttgctc cggactacaa aacagcagag
     3481 aaggctctgg aaaaagctgc aaatgctgcg atcggggtga tggagcgcgc cgatggtact
     3541 gggaaactgg tacgaaaaca gaaataacta ggtgtattat gactggtcac attcggcaat
     3601 gcccgattga tcacacctat actcttttga aaatttgtcc gaaatgtggt gctgcaactg
     3661 agtcagtgca ccctgcacga tattccccac aggataaata tggtaaatac cggaggatga
     3721 tacgcgaatg gaacaagtaa atgtgcgctg gtatgtagaa gatgtatctg cccactctgc
     3781 aagcgtggta atagctggtc ttccgggtgt tggccatgtg ggaaaactgg tggtggatca
     3841 tctggtccgt gcactttcca cagtgaaggt tgcggagata acttctacac tgtttccgcc
     3901 gcagatgtat cttgactgcg atgctgttct tcgccttccg cgcaacgaga tattttttgt
     3961 gccaggaacc gatgaacacg atccggtgct tttcctcgcg ggtgactgtc agagtaccag
     4021 tgccgaaggg cattacactc ttgcggaagg atatctgaga gtgtttgatc tgctgggcgt
     4081 ccgccgcatc tatacactgg gcggttacgg tgttggaaga atggtcgaac atccgcgggt
     4141 actttcagca ctgagttctg catccctcaa agaggaagta cttgcagccg gtgcagtgat
     4201 aaatcccgag gaaccggtag gaggcattat cggtgccgca ggattactta ttacgctcgg
     4261 ccgcctgcac gggatggaag gagttgcgct tcttggtgag acctccggtt acttggtaga
     4321 cccagtgagc tcaacagcag tactggatgt actggaacga ctaacacaca ttcatgccga
     4381 tcggacggaa ctgacggatc ttgctgagca gatgatgact gatattgcag caattgcaaa
     4441 tgctgcaacg ccaaaacctc cggacgatct cagctacatc gggtgagacc tatgcagaaa
     4501 aatacagatc tgcatctgca ctcctgtttc tccatggcaa cgtctccgga catggtgccg
     4561 gaacacattc tggcgggatg cagaatcaaa ggcatcgatg ttatcggaag cggcgatgca
     4621 ctgcatcctg aatggcgcaa aatgtgggag ccgtatgtgg acaatgaatt tgggattacg
     4681 gtgataccgc aaaccgaagt ggaggactcc tcacgtgtac atcacgtaat tctcgcagaa
     4741 acatttgatc agtttgcgga gctgcaggac atgttcgaac cggtatgcgg gcatcttact
     4801 accgcaggga gaccacatct tcagctggga ggagaagaga tcgcaacagc agtccatgac
     4861 gtgggaggaa tgattggtcc tgcacatgca tttactccgt ggacgtcatt gtttgcagca
     4921 ttcacatccc cttccgaatg ctatggggaa gagacgtttg agttctgtga gcttggactc
     4981 tcagcagatt ccacgtacgg tgcaggtatt gcagaatttg ccgatgttcc attccttaca
     5041 aactcagatg ctcacagccc gacaccaata aaactcgggc gcgagtttac acgaatggat
     5101 ctttccacag actcgcctaa atcagttctc gcaggaattt cctcaggaaa catcatcctg
     5161 aacgccggat tttttccgga agagggaaaa tacaaccgta cggcatgtac tcgctgttac
     5221 caacagtttt cacttgcgga ggcagagcac ttccactgga aatgcccgca tgacaacgga
     5281 agaatcaagc ttggtgttcg tgaacgcgca gaaaaactct ccacacttgc agaaccaaca
     5341 caacgtccgc catatctgaa aatgattcct ttgggagaga tgattgcacg cgtacttgga
     5401 acggcctcac caaacacgaa aaaatgcaaa gaactgtaca acagattcgt cgaaacattc
     5461 gacaatgaga ttgcagttct gctcgagatt cccaaacagg atctctgttc cgtatcatgt
     5521 gaagtggcag atgcagtgat aaaaatgcgg gagggacagg tcacactctt cccaggcggc
     5581 ggcgggaaat acggatcatt tgcattctga atatttacgg ctgacaaaat gaaaacaatg
     5641 ggcagggctc tcactgggga agcagctcag ctgcgatccg ttcctcggca atgttgatgt
     5701 atccttcatc gatctcaaac ccgataaacg ggatcccaag acgaactgcg gcaacagcag
     5761 tactgccaat acccataaac ggatccatga caaggcggca acggctgaca ccgtgatcac
     5821 ggatacacat catcggcagc tgaaccggaa aggttgccgg atggggacgc tcctcgcgga
     5881 cggtttcata ggggatgaac cacgtgttgc cgcgatcgcg gatatcctgt tttttttgta
     5941 cccatctgcg gacattgctt ttgtcctgat agggaacacc gtttgcgagc cgatcgatcg
     6001 gaacgtcacc ggttttggta aagtgaaaga tgaactcgtg gcagtcatta tggaaccgat
     6061 gactgttgat cggcttgtaa tgaccaaccg cgacaccatt ctcgggaatc tttgcattgt
     6121 agaactttcc catgtcctct ttaggaagag caatggattt gatccaatgg atcatattct
     6181 gaagaatgaa cccggatctc cggaactcct gcgcaacgtc catgggaatc cacggatctt
     6241 tgagactgcc tccaacattg agatacagag agccctcatc agagagtaca cgtatcgcag
     6301 catcggcaac atctttcatc caagagagat acccgttacg cggaacggtg tcatcatagg
     6361 tggtgtaaag cttgccaagg ttgtaaggtg gagaggtcac gataacatca acagatccgg
     6421 cagaaagccg gttcataccg gtgatacagt cacagtgatg aataattccc agagagctca
     6481 tggtcagaag atcttttcta tggtgatatc gtaggtgatc gtttctccgg caagcggatg
     6541 attcgcgtcc accgtgatgc ttttatcggt tacaccaacc accgtgacaa gacaaggctt
     6601 tttcaggact tcgactcgga tgatctctcc tgcaaccggt tcgtgatcaa gactgagttt
     6661 atgccgttta atggtgacaa cgagtttctt gtggtacctg ccgtaagctt tctccggagg
     6721 aagcacaacg gtgactgtct caccctcgga cttgccaata agagcctctt caaatgctgg
     6781 attgatctgt ttttttccga gcgtgaccag taccggttta ccagagcgag tatcctcaaa
     6841 gatttcccca tctggacgac ggctggtgaa atgaagaaga agcgtacttt ccgcagtgac
     6901 accactcatg attattctgt atgttttgaa tgtacattag tatttgtccg gacaaaaagg
     6961 agggattggt tactcccgcg gaatgtggca aacaccgaag tcacccttct tgattctccg
     7021 aagcgagagc cagaagaacg tcgccacaat tccaatggtc aggagcgggg caaaccaaat
     7081 tgggatgtgc aagccgaacc cttcaccgca catgataagg ccaagtgtaa agatagaata
     7141 catcgcacca tttttcagat aggcatatct tctgatattt tcgatgttcc ggacggttaa
     7201 ctcacgcaca acaactgcgc cgataccatt tccaatcaga atcagcggga cagacatggt
     7261 aaatgcaaag gcgccgataa caccatcaat agaaaatgtt gcgtcgatta cttcaagcag
     7321 catcaattta ctcatgtccg attttgttga agtaccggct tcaagctccg cagacttctt
     7381 gtcggcaaag agcctgaacc cctgcatgat gaaaaagatt gcagaacctc ccactgctgc
     7441 caccgcaaga agagcattga tctgtgctgc ggcatagcag atcagaaaca gaacaacagc
     7501 tgcaacagca tagaaccagg gaccctgacg ggacatggca cgctctcctg gaacaataca
     7561 gttcttctcc tcggccatca accagtggag gaaaaggaag atcatgaaga tccctcccgc
     7621 caaaaggagg aacggtgcac tggcctcaat tgcagccgca gcagtagcat cgtcggagaa
     7681 cgttgcagtc agtgctccaa tcggaccaag agaaggggtt gaaaaccaga tgataagcca
     7741 cggaagcaca ccgcggatac caaaaactgc aatgataata ccccaggtca ggaaccagcg
     7801 gcgtgcccac gctttcatgg tggacaaaat gtctgcattg atgattgcat tgtcgatact
     7861 agccacagtt tcaaatgcga cgaggccgac aatcacgaga acagcgtagg caatctccat
     7921 gctaactaac tgaggacatg taggggctga tagattatta atatcgctgt ttggtcaagt
     7981 gctgtatctt atctgatagc ccgcatgtgc tgatgaatat atactataga aacggattga
     8041 gataatggta tgaaaaagta tctccagacg gttctggttt gtctgtgcct aatttcagca
     8101 ggcggtgtga caacaatgtc agtatgtgca gagaaggaga tggaacggac aacccattca
     8161 gaaccagcag gagcaacaat gactgtttcc gagaggcagg ggggagagat ggaatggcag
     8221 tctcccagaa cggaactgga ggaatgattc agagatgaac gttctttccc tttccgatga
     8281 tgaatcggcg gcagttattg acagaaccat ccagtccctt cagaaggcaa cgagtactgc
     8341 tgacgcagtg aaaagaatcg ggtctgcttt atcccagtac acggtctttg atcttcagta
     8401 cataggagga tttatccgcc gcgaagtaaa caagctgcca aatccatacc ggagagtata
     8461 cctaccttac agtttggatc tgctgaatca gtatcacgtg tttatgcaga tgtaccgaac
     8521 gcagaagctt cccgatgaac caataaagga tcctacgctc tggagtgagt actgggaaat
     8581 tgcacgcgta cagtgttttg ttcgatccgg gccatcggat gatccgtttc cacagatgga
     8641 acatcccctg agcaaatttt tttaccggct cgtatacgga tatgtgatgc tgattgcaaa
     8701 tggatacgga cacccaatcg gtatgccgtt tccgggaggg gcaacagtcc atcgcgaggg
     8761 agaacaggta ctttgtccaa tcagagataa agagagggat cttcccgcag cattatgtaa
     8821 tttttgtccg gcagaacagg atccgcgata caaataattc ttccaagtaa agagaaaagc
     8881 aaactcagga atgtacggat atatagcagg cacacaccat acaatgatcg aattccgatg
     8941 tatgaactga tacacgcacg cgggaatacg tattatattg actgcccggc aaagatgggg
     9001 c
//
LOCUS       DALTZZ010000220         3277 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_35911,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000220 DALTZZ010000000
VERSION     DALTZZ010000220.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35911"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="fdhD"
                     /locus_tag="O0X12_04240"
     CDS             <1..843
                     /gene="fdhD"
                     /locus_tag="O0X12_04240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HJK53398.1"
                     /translation="LIGFVREGRFTVYTHPERITELNPEAGEPQPAKNCGVLAAPLRG
                     IRYREGKSIPVEDAAVAEDLVTLFLNGKEFIRTVASGEHLRELAVGFFVDAGLVCSAS
                     DILSVRTEGANVFVEASCTACVAGELESAGGFTPLHARGRAHPGGEITPEEIFVIRDA
                     INAEVWDATGGLHCAVLFHDHRVVFLASDIGRHNAVDKVIGYMILEGLNPQECVIGST
                     GRQPAGMVMKAANAGVPIVVSRAAATDAGIATAKECGVTLICFTRPPRFTVYAHPQRI
                     TGIL"
     gene            complement(946..2082)
                     /locus_tag="O0X12_04245"
     CDS             complement(946..2082)
                     /locus_tag="O0X12_04245"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004809 - tRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HJK53399.1"
                     /translation="MEYRQVTEGATTFEAPVQDEQTAFPPGTAPVFYNTKMEFNRDMT
                     VLLLSCISPENYLDAMAATGVRGLRVSHETGIPAIINDRDPAAVAMIEENAERIGGDI
                     RVICDDANRLMCTERFDAIDLDPFGTPAPFLDAASRSAKRYLFVTATDTAPLCGAHLK
                     AGLRRYFATPKNTEYHAEVGLRMMMGAMAKELVKYDRGMEPILSFAKNHYFRSHVRVL
                     GRVTAAEATMDQIGFVMQCPKCLYRAEQKGSLLPRSHTCPYCGAETEAIGPLWMGPLQ
                     DKKIISSLLEKLPSMQFGTAKQMDKLLSQLLREPDTCSFYDYHVVSRNLRVSPPAMDE
                     MLDGLNDLGYYTVRTHFSVTGIKTMAPLHVIEAWILAWNKERNL"
     gene            complement(2101..2175)
                     /locus_tag="O0X12_04250"
     tRNA            complement(2101..2175)
                     /locus_tag="O0X12_04250"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2138..2140),aa:Arg,seq:cct)
     gene            complement(2363..2623)
                     /locus_tag="O0X12_04255"
     CDS             complement(2363..2623)
                     /locus_tag="O0X12_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HJK53400.1"
                     /translation="MGEVAVILKVMPESPEVDMEKLQADIRAKVSGIQDMRVEPIGFG
                     LSAIKIAMITEDEEGSGDKIEGLFAGIAGIDRAEIESLNRML"
     gene            complement(2633..2794)
                     /locus_tag="O0X12_04260"
     CDS             complement(2633..2794)
                     /locus_tag="O0X12_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HJK53401.1"
                     /translation="MAATKCTSCNAPLAECGATEFKCPDCGEVIYRCAYCRKQSIKYT
                     CPKCGFQGP"
     gene            complement(2862..>3277)
                     /locus_tag="O0X12_04265"
     CDS             complement(2862..>3277)
                     /locus_tag="O0X12_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HJK53402.1"
                     /translation="MAVSAMDQYGWFLSTFGVAVDDVCSMPLAGAKILLPYRVLRDAD
                     PLPHSWDVTSDTISAWIAGKLGCPLILLKSVDGVFLGGDLLEEISADIETDVIDPCCR
                     DVLRRYAVPAHIINGRCPDRLSAYLQGDCVPGTRL"
BASE COUNT          880 a          781 c          824 g          792 t
ORIGIN      
        1 ctgatcggat tcgtacgcga aggcaggttt acagtataca cacatcctga gcgaatcaca
       61 gaactaaatc cagaggcggg agagccacaa cctgcaaaga actgtggagt acttgcagca
      121 ccactccgtg gtattcgata tcgagagggt aaatcaattc cggtagagga tgcagcagtt
      181 gcggaggatt tagtgacgct atttctgaat ggaaaagagt ttatcagaac agtggcgagt
      241 ggtgaacatc tgcgggaatt ggcagtgggt ttttttgtgg acgcaggcct ggtgtgctct
      301 gcctcagata ttttatcggt gcgcacagag ggagcaaacg tatttgtgga agcatcatgt
      361 acagcatgtg ttgccgggga gctggaatca gcaggagggt ttacaccgct gcatgcgcgc
      421 ggcagggcac atcctggagg agaaattaca ccagaggaga tctttgtgat ccgggatgca
      481 atcaatgcag aggtgtggga tgcaaccggc ggtcttcact gtgcagtcct gttccatgat
      541 caccgtgtgg tattccttgc atccgatatc ggacggcata atgcagttga caaggtaatc
      601 ggctacatga ttcttgaggg acttaatccc caggaatgtg taattggatc tactggaaga
      661 cagccggcag ggatggttat gaaggcagcc aatgcaggag ttccaatcgt ggtatcacgt
      721 gcggcagcaa cagacgccgg aatagcaaca gcgaaagaat gcggggtaac actgatttgt
      781 ttcacccggc caccgcgatt tacagtttat gcgcatccac agagaatcac agggatactc
      841 tgaggtagag agaaccgttc gcttcaggga tttgcagatc tttctttttg gaactgggaa
      901 tgtttgaata catcagctcc tggttgtact tcctctgcaa caaatctaca gattccgttc
      961 tttattccat gcaagtatcc atgcttcgat gacatgcagt ggggccatgg ttttgatacc
     1021 ggtgactgag aaatgagttc tgacagtgta gtatccaaga tcgttcaggc catccagcat
     1081 ctcgtccatt gcaggagggg atacacgaag gttgcgggag acaacatggt agtcgtaaaa
     1141 gctacaggta tccggctcac ggagaagctg actgagcagt ttgtccatct gctttgcggt
     1201 accaaactgc attgaaggaa gtttttcaag aagcgaggag ataattttct tgtcctgaag
     1261 cggacccatc caaagcggac ctatggcttc tgtttccgcg ccacagtaag gacaggtgtg
     1321 agagcggggg aggagagaac ctttctgttc tgcacgatag aggcatttgg gacactgcat
     1381 tacaaagcca atctgatcca tagtagcttc cgcagcggtg acacgaccaa gtacacgcac
     1441 gtgagatcgg aagtagtggt ttttggcaaa actaagaatc ggttccatgc cgcgatcata
     1501 tttaacgagc tcttttgcca ttgcacccat catcatgcga agtccgactt ctgcatggta
     1561 ttcggtattt tttggtgtgg caaagtaacg ccgcagacct gctttgagat gtgccccgca
     1621 aagaggggca gtatccgttg cagtgacaaa gaggtagcgt ttcgcggaac gtgatgcagc
     1681 atcaaggaag ggagcaggag taccgaacgg atcgagatca atcgcatcga agcgttcagt
     1741 acacatgagc cggttggcat catcacagat aacacggata tcaccgccaa ttctttctgc
     1801 gttctcctca atcattgcga cagcagcagg gtcgcgatcg ttaatgattg caggaattcc
     1861 ggtttcatgg gatacacgaa gaccgcggac accggttgct gccatcgcat cgaggtaatt
     1921 ttctggagag atgcaggaga ggagaagaac ggtcatgtca cggttgaact ccatctttgt
     1981 gttgtagaag acaggagcag ttcctggagg gaatgcggtt tgctcgtcct gtactggggc
     2041 ttcaaaggtg gttgcccctt ccgtgacctg tcgatattcc ataatgcatc tccaaaaaga
     2101 cggacatgta gggattcgaa ccctagatct tcagcttagg aggctgatgc catatcctgg
     2161 cttggctaca tgtccagaag gtgaggatat atgtttggag agacgcgggt atatctttta
     2221 cgtaagcatg atcctaaaat gaaccaaacc ttctgaaaca tacagacaat acacatggta
     2281 tcctccattt gttttgatgc tgcaaaaccc caaacacgta ccgcagcgaa acactatcga
     2341 agtgtgaaaa gaaaataagt atttagagca tccggttcag agattcaatc tctgcacgat
     2401 cgatgcctgc aattcctgca aagagcccct caatcttgtc gccggaacct tcctcatcct
     2461 cggtgatcat ggcgatcttg attgcagaga ggccaaatcc aatcggctca actctcatat
     2521 cctgaattcc ggaaaccttt gctctgatat cagcctgaag tttttccatg tcaacttccg
     2581 gagactccgg catgaccttt aaaattactg caacttcgcc catattattt cctcatggtc
     2641 cctggaatcc gcacttcgga cacgtgtatt taatgctctg cttacggcag tatgcacaac
     2701 ggtagataac ttcaccgcag tcaggacact tgaactcggt tgcaccgcac tctgcgagtg
     2761 gggcattaca tgacgtacat ttcgtagctg ccattcgatc aaactctcta gattattagc
     2821 tccctacaat tataaacgtg tggcctctgg cacgcacaat atcacaacct cgtaccggga
     2881 acgcaatctc cctgcaaata ggcagacagg cgatccggac atctcccatt gataatatgt
     2941 gctggaaccg catatctgcg cagaacatcc ctgcagcagg gatcaattac atcagtctca
     3001 atatccgctg agatttcctc aagcagatct cctcccaaaa aaactccatc aactgatttc
     3061 aaaagaatca gagggcagcc cagcttgcct gcaatccatg cagatattgt atccgacgtc
     3121 acatcccatg aatgaggaag aggatctgca tcccgcaaaa cccgatacgg aagaagaatt
     3181 tttgcaccag ccagaggcat tgaacaaaca tcatccactg cgacaccaaa cgttgacaaa
     3241 aaccacccgt actgatccat tgcagacaca gccatcc
//
LOCUS       DALTZZ010000221         4861 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_36042,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000221 DALTZZ010000000
VERSION     DALTZZ010000221.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36042"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(180..1598)
                     /locus_tag="O0X12_04270"
     CDS             complement(180..1598)
                     /locus_tag="O0X12_04270"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HJK53403.1"
                     /translation="MQKREIVARFLEAGLLVHPAVVTYISESGGSGIIEGIISSLPSS
                     VSVVTPKDVPGMTAARVDKLEVGVSALPEILAGRAGSSVPTATPEESFALFRDRYDAL
                     AAMMRPRVSPIPIEALVKQNNRFADTDIGAIGMVVESSTSAKGHRIVEIEDPTGTIKV
                     LFNKNREGFDEAEKIIPDEVIGVRGKLARDTGGGGGMIFSDTLFRPEIPLTHTISPSK
                     EPGKAVLISDVHVGSDTFLPDAWDRFASWLENTPEVKYLLIAGDVVDGIGVYPNQDKE
                     LHIKTIYEQYDAVGEMLSSLPSHLQIVLSPGNHDAVRAAEPQPVLPEEFRTRFPSNVT
                     FVENPAVVNIQSVNVLMYHGRSIDDLIKFIPGASYDHPGEIMEAMLKRRHLGPIYGQR
                     TPLLSTPTDHLVIRDVPDILHTGHVHITDVISYRGVLGVNSGTWQGQTSFQKQMNINP
                     TPGEAIVVDLFTMGYEKMCFHT"
     gene            complement(1655..2818)
                     /locus_tag="O0X12_04275"
     CDS             complement(1655..2818)
                     /locus_tag="O0X12_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJK53404.1"
                     /translation="MRSFVSRMAAEIPASGIRKCFDLLLTMGNVISLGVGEPDFDAPW
                     NVSQAAISSIEKGMTMYTSNRGLAELRELLSADLDQRYHTKYCPETEMIVTTGVSEAL
                     DVAVRAVVDPGDEVLVVDPCFVSYQPEVLMAGGKPKALPCRAQDQFKVTPEALMESVT
                     PKTKVLMMNFPNNPTGGVMGADDLKAIADIVIDHDLVVISDEVYSELTYDGHHVSTAS
                     IDGLWDRTITLNGFSKAYAMTGWRLGYICAPKEICDAALKIHQYVMMSAPTAAQFAAI
                     EALKNGEEAKNEMVAEYRMRRNLFVKGLNDVGLPCHLPKGAFYAFPSIEGTGLTDEEF
                     AEQLLKEQHVAVVPGSAFGESGRGHVRTCYAVDRSKLSEAVRRIGTFVESLHS"
     gene            complement(2831..3322)
                     /locus_tag="O0X12_04280"
     CDS             complement(2831..3322)
                     /locus_tag="O0X12_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HJK53405.1"
                     /translation="MDEKDTVILRELQKNSRISARDIAVMLEVDEREVEDRISALHAA
                     GVLRRCTAVIDYSAAGVDEVAVILSLKVTPERGLGYDGIAAQIARFPQVESILLLTGT
                     YDLQVIIRGQSMADVSRFVAEQIASIEGVRETMTQIVMRTYKEQGVEMSRPGSRDRQL
                     ITF"
     gene            complement(3352..4608)
                     /locus_tag="O0X12_04285"
     CDS             complement(3352..4608)
                     /locus_tag="O0X12_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HJK53406.1"
                     /translation="MSDEPFKSTGLFKKYTIKNKIFQNREVLRHSYSPRELPHRVDQI
                     DSIAVILAPALQGATPSNILIYGKTGTGKTATVKFVGSELENESSEFSPCRMVHLNCE
                     TIDTQYRVLAQIANHISGHDLKPSDRAKNTIPATGWHTDQVYSELKNVLEQAGGLQII
                     VLDEIDKLVKKSGDDTLYNLTRINSDLKNARVSIIGISNDLTFKDFLDPRVLSSLSEE
                     ELVFPPYDAVQLQDILHQRAEMAFLPDAVSEDVIALCAALAAQEHGDARRALDLLRVS
                     GELAERESSDKVLESHVRRAQENIETDTMSECVQTLPRQGKMVLCAMLLVSSSGQKVF
                     TSGSVINIYRELARELDIEPLTHRRVSDLINELNMLGIVTARVVSHGRHGRTTEIYFN
                     SPTNQIRTVVMNDPSLRECSILQPLL"
BASE COUNT         1272 a         1280 c         1165 g         1144 t
ORIGIN      
        1 atccatacga atcaaaaacc tccggatacc cggtttggtt tctctctata gtacaggaca
       61 gactacctaa taatactact gttaaaagag tttccggtat tcactgaaac aaccccgtac
      121 gaaagagttt cgattattga cgaacaggaa gcgcagaaag aacctaggaa ggtgatgggt
      181 tatgtatgaa agcacatctt ttcgtagccc atagtaaaca gatccaccac gattgcttcc
      241 cctggtgtcg ggttaatgtt catctgtttc tggaaagagg tctggccctg ccaggttccc
      301 gagttcacgc caagtacccc ccgataactg atgacatccg taatatggac atgaccggta
      361 tgaagaatgt ccggaacatc ccggatcacc agatgatccg tcggagtgga aagcagcggt
      421 gttctctgac catagatcgg accgagatga cgacgcttca gcatcgcctc catgatctct
      481 cccggatgat catagcttgc cccaggaata aacttgatca gatcatcaat actgcgtccg
      541 tgatacatca gaacattcac cgactggatg tttacaaccg cagggttttc cacaaacgta
      601 acattgctcg ggaaccgagt tcggaactct tcgggaagaa ccggctgggg ctctgcggcc
      661 cgaaccgcat cgtggttgcc gggtgaaaga acgatctgca gatgcgacgg aagggatgaa
      721 agcatctcac cgaccgcatc gtactgttca taaatcgttt tgatgtgcag ctccttgtcc
      781 tgattcgggt acacaccgat accatccacc acatctcctg cgatcaggag atacttgacc
      841 tctggcgtgt tctccagcca cgaagcaaac ctgtcccagg catccggaag gaaggtatcg
      901 ctcccaacat gtacatccga gatgagaaca gctttgcctg gctctttgga cggagagatc
      961 gtatgggtca gcggaatttc cggacgaaac agggtgtcag aaaagatcat gccgcccccg
     1021 ccaccagtat cgcgggcaag ttttccgcga acgccaatga cttcatcggg aatgatcttt
     1081 tccgcttcat caaaaccttc ccggtttttg ttgaacagta ccttgatcgt accggtagga
     1141 tcctcaatct ccacaatacg atggcctttc gcactggttg aactctcgac aaccataccg
     1201 atagcaccaa tatccgtgtc ggcaaaccgg ttgttctgtt tgaccagtgc ctcgatcggg
     1261 atcgggctga cacggggccg catcatcgca gcaagagcat cataccgatc acggaacaaa
     1321 gcaaaggact cctccggtgt tgccgtcgga acagatgacc ccgcccttcc cgcaagaatc
     1381 tccggtaaag cactcacacc aacctcaagc ttgtcaaccc tggccgctgt catacccggc
     1441 acatcctttg gtgtaaccac cgaaacggag gaaggaagcg aactgataat accttcaatg
     1501 ataccggaac cgccgctctc actaatatag gtaacaaccg ccgggtgaac cagcagtccc
     1561 gcctcaagga atcgtgcgac gatctctctc ttctgcatgg aaaatcagga ggagtatata
     1621 aactcctcca ctcgaaacaa aggggttaaa tacttcagct gtgcagcgac tcaacaaatg
     1681 ttccgatacg acggacagcc tcggaaagtt tcgatcgatc caccgcataa catgtccgta
     1741 catgaccgcg accgctttca ccaaacgcgc tgccgggcac taccgcgaca tgctgttcct
     1801 ttaacagctg ctcggcaaac tcctcatcgg taagacctgt tccttcaatg gaggggaaag
     1861 catagaatgc accctttggc agatggcagg gaagaccgac atcattgagg cctttgacaa
     1921 agaggttgcg ccgcatccgg tactctgcaa ccatctcatt ctttgcctct tcaccattct
     1981 tcagtgcctc aattgctgca aactgcgcag cagtcggtgc agacatcatc acatactgat
     2041 gaatcttcag tgcggcatcg cagatctcct tcggcgcaca gatgtagcca agccgccatc
     2101 cggtcatggc atatgccttg gaaaaaccat tcagtgtgat tgtccgatcc cacaggccgt
     2161 caattgaggc agtagagaca tgatgaccgt cataggtcag ctccgagtac acttcatctg
     2221 agatcacaac aagatcgtga tcaatcacaa tatcggcaat ggccttcaga tcatcagcac
     2281 ccattactcc gccggtagga ttgttcggga agttcatcat cagaactttc gtcttcggtg
     2341 taacagactc catcagggcc tcaggtgtta ccttaaactg atcctgtgcc cggcagggga
     2401 gagccttcgg ttttccgcct gccatcagca cttcgggctg atatgagaca aaacaggggt
     2461 ccacaacaag aacctcatca cccggatcaa ccaccgcccg aacagcaaca tcaagtgcct
     2521 cggaaacacc ggtcgtcacg atcatctcgg tctccggaca gtattttgta tgataacgct
     2581 gatccagatc ggcagaaaga agctcccgca gctcagcaag accgcggttt gacgtgtaca
     2641 tcgtcatgcc tttttcaatt gaggagatgg ccgcctgaga gacgttccaa ggagcatcaa
     2701 aatccggctc gccaacgcca aggctgatca cattgcccat ggtgagcagc agatcaaagc
     2761 acttccgaat gcccgacgca gggatctccg cagccatccg tgatacaaaa cttcgcatga
     2821 tcccccgcct tcagaacgtg atgagctggc ggtcgcgact acccggccgc gacatctcca
     2881 ccccctgctc tttgtatgtc cgcatcacaa tctgcgtcat cgtttctcga acgccctcaa
     2941 tggatgcaat ctgttcggca acgaaccggg agacgtcggc cattgactga ccacggataa
     3001 taacctgaag atcatacgtt ccggtaagga ggagaataga ctccacctgg gggaagcggg
     3061 caatctgggc ggcaatgcca tcataaccaa gtccgcgttc cggagtaact ttaagggaca
     3121 gaatgacagc cacctcgtca actccggcag cactgtagtc gataaccgca gtacagcgcc
     3181 ggaggacacc tgctgcgtgg agtgctgaaa tacgatcctc aacttcacgt tcatcaacct
     3241 ccagcataac agcaatatct cttgcagaga tacggctgtt cttttgcagt tcccgaagga
     3301 ttactgtatc cttttcgtcc ataatttttc accttgtgtg atgcagaggt ctcagaggag
     3361 cggctgtaag atactgcact ccctaagact tggatcgttc attactactg tacgtatctg
     3421 attagtggga ctgttgaaat agatttctgt tgtcctgcca tggcgtccgt gtgatacgac
     3481 ccgggcggta acgataccca gcatatttag ttcgttaatg agatccgaaa cccgcctgtg
     3541 tgtcagcggt tcgatgtcaa gctctctggc aagttcacgg tagatgttga tgacgctgcc
     3601 gctggtaaac actttctggc ctgaggagga gacaagaagc atggcacaca gtaccatttt
     3661 cccctgacga ggaagagtct gaacacactc actcatggta tcggtttcga tgttctcctg
     3721 ggcccggcga acatgggact caagaacttt gtccgaggac tcgcgctcgg caagttcccc
     3781 ggagacacga agaagatcaa gtgcacgtct ggcatcacca tgctcctggg cagcaagtgc
     3841 tgcacaaaga gcaatgacat cttcactgac agcgtcaggg aggaaggcca tttcggcacg
     3901 ctggtgtaaa atatcctgca actgaacggc atcataagga gggaacacaa gttcctcttc
     3961 agagagagag gaaaggactc tcgggtcaag aaagtcctta aaggtaagat cattggatat
     4021 accgatgata ctgacacggg catttttgag gtcactgttg atccgcgtca gattgtaaag
     4081 cgtgtcatca ccgcttttct tcacgagttt gtcaatctca tcaagtacga tgatctgtaa
     4141 acctccggcc tgttcaagaa cattcttgag ttcggagtac acctgatcgg tgtgccagcc
     4201 ggttgcagga atcgtgttct tggcgcgatc acttggtttg agatcatggc cgctgatgtg
     4261 attggcgatt tgtgcgagta cacggtactg agtgtcaatg gtttcacaat tgagatgaac
     4321 catacggcat ggagagaact cggaactttc attctccagc tcggatccaa caaatttgac
     4381 ggttgcagtt tttccagtac cggtttttcc ataaatcaga atgttagaag gagttgcacc
     4441 ctggagagct ggggcgagaa tgacagcaat ggaatcaatc tgatcaacac ggtgcgggag
     4501 ttcacgcgga ctgtaactgt ggcgaagaac ttcacgattt tgaaatattt tatttttaat
     4561 ggtatatttt ttgaaaagac cagtagattt gaaaggttca tcagacatga tacatcagat
     4621 ccgtaaagta ccttacgtct tttttcctat taataaaccg ctgtttccac tggagaatag
     4681 ttttatggta tcatgagaaa gggccataat tacaaacaaa gataggtcag aatactgact
     4741 gaaacttcag gaaaacgttg tacatgtcaa agtcagtccc ccaccccttt atttcgagtg
     4801 gaactttgat cgggtgtttg caaaaaacgt ggaaatcgcc aagatttacc ttgttacatc
     4861 c
//
LOCUS       DALTZZ010000222         2179 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_36045,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000222 DALTZZ010000000
VERSION     DALTZZ010000222.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36045"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="O0X12_04290"
     CDS             complement(<1..406)
                     /locus_tag="O0X12_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53407.1"
                     /translation="MKIAHPILIGALVLTLLCAGVTAPAAAEVTGQISVINLTMDPEV
                     LMPGDTATVTAVIKNSGTTSVSIGRAMIMGVNEVYSAEDKAYNSVGMLGPGDEMTFTF
                     PITSRGAVGTFYPMLYLDFLDAGLTSLKYTFAV"
     gene            608..1603
                     /locus_tag="O0X12_04295"
     CDS             608..1603
                     /locus_tag="O0X12_04295"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HJK53408.1"
                     /translation="MNSSASRITGSLLGGAIGDALGYPVKFTSLEEIERRYGTNGVPA
                     RLVPNPHTGLACFSDTTQLTLFTAEGLIYAAKTGMNPVKSVYRSYLQWLYTQDGGLIQ
                     SASTGLLAHHEMYVTRAPAATTLAALHSGAMGSIDMRINTSKAADGMMRTAPAGYIAE
                     HDSARILGERIAALTHGHPDGYRAAGAFSALISYLIDGTALWDAATTVRDETAKLSWG
                     TETAAKLSDAIDLAEKDIPDADAISQLGTGKTAERALSVGLFCGLRHEDNFPETVRAA
                     ANHGGDSSVTASVAGQIVGACIGREAIPAELTERLELSAMINDYGMQMGSLRGCL"
     gene            1600..2094
                     /locus_tag="O0X12_04300"
     CDS             1600..2094
                     /locus_tag="O0X12_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53409.1"
                     /translation="MTAADEYFQKGMQAYLVRNFAESIEWFTKALAENPENVNYYYYR
                     GTSYQELEQYSEAVADYSAALDRFSGCVPIRYNRAEICRKLGHADRAAKDLTYIIVHA
                     DRTKGEGHWLALAYLNRGLARIDCGDLETGLVDFGKAEALAKELGDKILLAQIADELE
                     KSGL"
BASE COUNT          580 a          468 c          593 g          538 t
ORIGIN      
        1 ggacggcaaa ggtgtacttg aggcttgtta atcctgcgtc gaggaaatca agataaagca
       61 tcggataaaa ggtaccaact gcaccacggg aggtgatcgg gaaagtaaag gtcatttcat
      121 ctcccggacc gagcatacca acactgttgt aggctttgtc ttcggcagaa tagacctcgt
      181 taacacccat tatcattgca cggccaatag agacggaagt tgttcctgag tttttgataa
      241 ccgcagtcac agttgcggtg tcgccaggca tgaggacttc agggtccata gtaaggttga
      301 tcaccgagat ctgtccggtg acttcagcag ctgcaggagc agtgacccca gcacacagca
      361 acgtgaggac gagtgctcca atcaggatgg gatgggctat tttcattatt ggtttacctg
      421 tttttgtgtg attgtataac ctctttccct ttgtcctttt tagaaggagg ggtacttcta
      481 ttctgtcgtg cagaagttat acaacagaat tatttatcgt ttatcgataa atagttatcg
      541 taaatcatac ttgttttcaa gtatcgctcc cgacaatcct aatagtaagc aggacaaaca
      601 ttccagtatg aattccagtg catctcgtat taccggctcg cttctcggcg gggcgatagg
      661 tgatgctctt ggctaccctg tgaagtttac gagccttgag gagatagaaa gaaggtacgg
      721 aacaaatggg gtcccggcac gtcttgtgcc aaatccacat acagggcttg cgtgtttttc
      781 ggatacaaca cagttgacac tgtttacggc ggagggactg atttatgcgg cgaagacggg
      841 aatgaatcca gtaaagtcgg tgtatcggtc ttacctgcaa tggctgtaca cacaggacgg
      901 aggactgatt cagagtgcat caacaggact tcttgcccat catgaaatgt atgttacccg
      961 tgcaccggca gcaacaacac ttgcagcact gcattctggt gccatgggta gtattgatat
     1021 gcggataaac acaagcaaag cagctgacgg gatgatgcgt acagcacctg cagggtatat
     1081 tgcagaacac gattccgccc ggattctcgg ggaaagaatt gctgcactga cgcacggaca
     1141 tccggatgga taccgtgcag caggtgcatt ttcggcactg atctcatacc tgattgatgg
     1201 tactgcgctt tgggatgcgg caaccacagt gagagatgaa actgcaaagc tgtcgtgggg
     1261 aacggagacc gcggcaaaac tttccgacgc aattgatttg gctgagaagg atatcccgga
     1321 tgcagatgcc atatcccaac ttgggaccgg aaaaacggcg gagagggcgc tttcagtcgg
     1381 gctattctgt ggacttcggc atgaggacaa ttttccagag acagtacggg ctgcagcaaa
     1441 tcatggtggt gattcaagtg tgaccgcgtc agttgcagga cagattgtag gagcttgcat
     1501 tgggagagag gcaattcctg ccgagcttac agagagactt gagctttcag caatgatcaa
     1561 tgactacggg atgcaaatgg gatctttgcg ggggtgtcta tgactgcggc agacgagtat
     1621 ttccagaaag ggatgcaggc gtatcttgtg cgaaactttg cagaaagcat tgagtggttt
     1681 acaaaggcac ttgctgaaaa tccggagaat gtgaactact actattatcg cggaacgtcc
     1741 tatcaggaac tggaacaata ttccgaagcg gttgcagact actctgcggc gctggacaga
     1801 ttttccggat gtgtcccaat ccgctacaat cgtgcagaga tctgcagaaa gcttggacat
     1861 gcagatcgtg cagcaaagga tctgacatac attattgttc atgccgaccg aacaaaaggt
     1921 gaggggcact ggcttgcttt ggcatatctg aacagaggtc tggcgcgtat tgactgcggc
     1981 gatctggaga caggactcgt tgattttgga aaagcggagg ctcttgcaaa agaactcggg
     2041 gacaaaattc ttcttgcaca gattgcagac gaactagaga agagcggatt ataactaact
     2101 ttcttgaaaa aagattattg ggaatcagaa tcttccgttt cgtcctcatc ctctttttct
     2161 gcatcggaga gagcggaca
//
LOCUS       DALTZZ010000223         3340 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_36148,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000223 DALTZZ010000000
VERSION     DALTZZ010000223.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36148"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="O0X12_04305"
     CDS             complement(<1..705)
                     /locus_tag="O0X12_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HJK53410.1"
                     /translation="MHDPTIPNVNIGVVGHVDHGKTTLVSQLTGSWTDRHSEELKRGI
                     SIRLGYADATFYKCPKCEGADSWSNADTCPICGEKLEPVRSVSFVDAPGHETLMATML
                     SGSAIMDGAMLVIAANEPCPQPQTKEHLMALELTGIKNIVIVQNKIDVVPQKQAIENY
                     KQIKAFVKGTVAENAPIIPVSAQKKINFGVLIDALNTTIPDTERDADVPPIMLIARSF
                     DVNRPGSSWREIKGGVI"
     gene            881..1300
                     /gene="nikR"
                     /locus_tag="O0X12_04310"
     CDS             881..1300
                     /gene="nikR"
                     /locus_tag="O0X12_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HJK53411.1"
                     /translation="MVGDSELSRIGISLPQNLLDKFDGIIGLRGYSSRSEGIRDAIRS
                     YITYYEWMSDVKGERQGVITMVYDHDHRGLMQTITEIQHENRDTIQSTLHAHVNDERC
                     LEVILVRGDGAKLKEVAEKLMALKGVESVKLTTIPVN"
     gene            complement(1325..1606)
                     /locus_tag="O0X12_04315"
     CDS             complement(1325..1606)
                     /locus_tag="O0X12_04315"
                     /inference="COORDINATES: protein motif:HMM:NF021397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2098 domain-containing protein"
                     /protein_id="HJK53412.1"
                     /translation="MSTPAIGQTVRYGRTGTVGKIVAFVEENGFTFAELDSTGLYYRV
                     DQLTAISEVAHKETRHRDFKKDLEEEQKRFREMNESAWQNTDQSCEGGG"
     gene            complement(1701..>3340)
                     /locus_tag="O0X12_04320"
     CDS             complement(1701..>3340)
                     /locus_tag="O0X12_04320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HJK53413.1"
                     /translation="LIVLTIIILGSISVAIPSLFASAIGSITQFFATSAAQSTIAEMA
                     AWTMEGAVSSFGWGLLLAAGGFIYLLYRVWKHEEPGALFVLIWSALMIFATMQHVRWE
                     YYVAANIALLAAVFVGWTITFAEKDLQQMIGSKKPEERDSTPTKKGKKAAAKSSSGPD
                     MLKVGTCVIVVLVALVFVGTSAATAVQTGESYGKYGGTEADWISECTWMLTGTPETGV
                     DYLKIYDEEGFEYPSEAYGVMSWWDYGHYITTIAERIPNSNPFQAGVAGTYGAAAVLT
                     STNESAVMEKLDYLGTRYIMTDYQMAGAKFGAMAIWNDSTAQLTPYYYTLLQNSNGNL
                     QIVQAYTPEYYNTLTVKLQYYDGSMTEPGDVAVVETDSSANYGYPVITNVQGYATAEE
                     AQAAADAFNENAPATKHAYVIANPSGVATAYLPSTTVPALQHFRLVHESPNYVLANSQ
                     YTNQPVEGGTAWVKSFEYVQGAVINGEGTIEVNVTTNNGRTFTYRQESIDGQFIVPYS
                     TYGSSYDVKTTGPYTIVETGETFEVSEEAVMQGLTIN"
BASE COUNT          745 a          832 c          843 g          920 t
ORIGIN      
        1 aataactcct cccttgatct ctctccagga acttcctggt ctgtttacat caaaggatcg
       61 ggcgatcagc ataatcggcg gaacatcagc atcccgctct gtgtctggaa tggtggtgtt
      121 cagcgcatca atcaagactc cgaagttgat ctttttctgt gcggaaaccg gaatgattgg
      181 cgcattttct gcaactgttc ccttcacgaa tgccttgatc tgtttgtagt tttcaattgc
      241 ctgtttctgg ggtacgacgt caatcttatt ctgcacaatg acgatgtttt tgataccggt
      301 cagctccagg gccatcaggt gttctttggt ctgcggttgc gggcacggct catttgcggc
      361 aatgaccagc attgctccgt ccatgatggc agagcccgag agcattgttg ccatcagcgt
      421 ttcatgtccg ggagcatcca caaaggaaac tgatctcacc ggttcgagtt tttctccgca
      481 gatcggacag gtgtctgcat tagaccagga atctgctccc tcacatttgg ggcacttgta
      541 aaacgtcgca tcagcatatc cgaggcggat ggagatcccg cgcttcagct cctcactgtg
      601 gcggtcggtc catgagccgg taagctgact gaccagggtg gtctttccgt ggtcaacgtg
      661 gcctaccacg ccgatattca cgtttggtat tgtaggatca tgcaaagaaa ccaaaacctc
      721 ctcaacccta tataatcgac ctccaaacag atatatttgt gtggtagtcc tctcccgtct
      781 tctttgcaaa cggacaacag acttcgtatg acaaactctc gctctgcata agattatgta
      841 ataagagtaa taaaggaaca gcctgatata atactatatt atggtaggag actcggagct
      901 ttcacgcatc ggcatatctc ttccgcagaa tcttctggat aaatttgacg gaattatcgg
      961 acttcgcggc tactcttccc gctcagaggg tattcgcgac gccatccgca gttacatcac
     1021 ctattatgag tggatgtccg atgtcaaggg ggaacggcag ggagtgatta ccatggtcta
     1081 tgatcacgac caccgcgggc tgatgcagac gatcaccgag attcagcatg aaaaccgtga
     1141 taccatccag tccactctgc atgcccatgt aaatgatgaa aggtgtcttg aagtgatcct
     1201 cgtccgcggc gacggcgcaa aattgaagga agttgcagaa aaactgatgg cgctcaaggg
     1261 tgttgagtcg gtcaaactga ccactatccc ggtaaactga tttttcaaaa aatgggagga
     1321 gagtttatcc tccgccttca caactttgat cggtattctg ccaggctgac tcattcatct
     1381 cacggaatct cttctgttcc tcctcaagat cctttttgaa gtcacggtgc ctggtctctt
     1441 tatgggcaac ctcactgatt gcagtgagct ggtcaacacg atagtacaga ccggtactgt
     1501 caagttctgc aaaggtgaat ccgttctcct caacgaatgc cacgatcttc ccgactgttc
     1561 ctgttctgcc gtaccgcacc gtctgcccga ttgcaggagt gctcatgcta ctatgttgga
     1621 tggtgccgga gtataaagat gtggattgga tatcttgttg tctgaaaaaa gtggatctgg
     1681 taggataacc agtgtccggt ttagttgatg gtaagaccct gcatcacggc ctcttcagag
     1741 acctcgaatg tctcgccggt ctctacaatg gtataggggc ctgtcgtctt cacatcgtat
     1801 gaactgccgt aggttgagta cgggacaatg aactgtccgt caatactttc ctgacggtag
     1861 gtgaaggttc tgccgttgtt ggtggtaacg ttgacttcaa tggtaccctc tccgttgatt
     1921 acggcaccct gcacgtactc aaaactcttg acccatgcgg ttccgccttc caccggctgg
     1981 tttgtgtact gactgttagc cagcacatag ttcggggact cgtggaccag gcggaagtgc
     2041 tgcagtgccg gaaccgttgt actcggcaga tatgcggtag ctacaccaga cgggtttgca
     2101 attacatagg catgttttgt tgcaggtgcg ttttcattga atgcgtcagc tgctgcctgt
     2161 gcttcttctg ctgttgcata tccctgtaca ttggtgataa ctggataacc gtagtttgcg
     2221 gatgagtctg tctcaactac tgcaacatct cccggttcgg tcatcgatcc atcgtagtac
     2281 tggagtttga cggtgagtgt gttgtagtac tcgggtgtgt acgcctgtac tatctggagg
     2341 tttccgttac tgttttgcag gagtgtgtag taatacggtg tcagttgtgc tgttgaatca
     2401 ttccagattg ccattgcacc gaacttagca cctgccatct ggtagtcggt catgatatag
     2461 cgggttccga ggtagtccag tttttccatt actgcggact catttgtact ggtcagtaca
     2521 gctgctgcac cgtaggttcc ggcaacgcct gcctggaatg ggttgctgtt tggaatacgc
     2581 tcagcaattg tggtgatata gtggccatag tcccaccatg acataactcc gtacgcttct
     2641 gacgggtact cgaacccctc ttcgtcatag attttgaggt agtcaacccc ggtttccggt
     2701 gttcctgtga gcatccaggt acactccgag atccagtctg cttcggttcc gccgtatttt
     2761 ccgtagcttt caccggtctg aacagcggtt gctgccgatg ttccaacaaa gacaagggca
     2821 accagtacga caataacgca ggttccgact ttaagcatgt ctggtcctga ggatgacttt
     2881 gctgccgctt ttttaccttt ctttgtaggt gtactgtcac gttcctctgg ttttttgctt
     2941 cctatcatct gctgaagatc tttttcagca aaggtgatgg tccatccaac aaacactgcg
     3001 gcaagcaggg cgatgtttgc tgccacgtag tactcccagc gtacgtgctg catggtggca
     3061 aagatcatca gtgccgacca gatcagaaca aagagtgccc ccggctcttc gtgtttccat
     3121 acacggtaga gtaggtagat gaaccctccc gcggctaaca ggagacccca tccgaaggaa
     3181 ctgaccgctc cttccatagt ccacgcggcc atctcggcga tcgtggactg ggctgcgctt
     3241 gtggcaaaaa actgggttat ggatcctatt gcagatgcaa acagggaggg aatggctact
     3301 gagatgcttc cgagaatgat aatggtcagc acaataagaa
//
LOCUS       DALTZZ010000224         3001 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_36270,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000224 DALTZZ010000000
VERSION     DALTZZ010000224.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36270"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            33..1328
                     /locus_tag="O0X12_04325"
     CDS             33..1328
                     /locus_tag="O0X12_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HJK53414.1"
                     /translation="MSGRDVVVIGAGVAGIQAAMDIANHDIHVWLVEREPTIGGHMGM
                     LDKTFPTNDCSMCILSPKMAEVGRHPNITLLTLSEVEKIEGTAGDFTVTVTKYPRYIR
                     EEDCTGCGDCVNICPVEVYNKFDAGLGVRKAIYKPHAQVVPNLVVKDNLHCIECGLCY
                     EVCGKNAVLHVDEDRVKTLTIPAAAVVIATGYTLFDAQKKGQFGYLRYPDVITSIEFE
                     RMINAGGPTLGEVRRLSTGKTPKSVVFVQCVGSRDVSIGRNLCSCVCCMYALKNSMLI
                     KEHYPETEVTILYNDLRAYGKGYEEYAERAKQMGVNIVRAFPGEVQQTRNGLVLPLED
                     TETGEFKNLEAELVILSVGMEPEPDTVRLAKSLGLPLDQNNFLSSADMKLDPAGTIRP
                     GIYIAGAAVAPKDIPDSVISGGAAAMKATIDSYTSEVKE"
     gene            1325..2350
                     /gene="mtnA"
                     /locus_tag="O0X12_04330"
     CDS             1325..2350
                     /gene="mtnA"
                     /locus_tag="O0X12_04330"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HJK53415.1"
                     /translation="MTDETKTIWWNDENNSIMLIDQTKLPVEFSIIEVTTVERLAEAI
                     RRLEVRGAPALGVAGAFGVALSAVSCVSDAEFAETVAADAELLRSTRPTAVNLSWGID
                     KVLRSMENLPPEMAKFLAITAAKNIAAEDEKCCMLLGHNGASLLPQIGTVLTHCNAGA
                     LACSTWGTALGVIRSAHKMGKKISVVSCETRPLLQGARLTAWELAHDNIPVTSIIDSE
                     AAYLMRQGKIDCVVVGADRITRDAVFNKIGTYMHAVCAKHHNIPFYVAAPISTFDVNA
                     VEADIVVEERTRDEVAAFWGKPTVPAGVPVINYAFDATPLDLVTAIITEKGVFYPPYD
                     FSSLGQM"
     gene            2354..>3001
                     /locus_tag="O0X12_04335"
     CDS             2354..>3001
                     /locus_tag="O0X12_04335"
                     /inference="COORDINATES: protein motif:HMM:NF014078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HJK53416.1"
                     /translation="MSVTLSLSIFNNPYWDSVALMLGDLALLVFFVWLIFLMITVGLF
                     VLSVRKKHLYCPWLLRPAYLLLKGAVRTGCRMFGIDDSEIITVMIRLENDVNRNAFAA
                     VPVEERVIFLPHCLRSAKCPAHLTPLGIKCLDCGKCGLGMATNALSDAGYKVFIIPGS
                     TYIKRLLKRFHPRAMIGVGCLMEIKQFQELARKIDMTAMGVVMTSDGCVETTVEWE"
BASE COUNT          628 a          753 c          906 g          714 t
ORIGIN      
        1 cacctatgtg gatgtcggca ggagataccg gaatgagtgg gcgtgacgtt gttgtaatcg
       61 gtgccggtgt tgcaggtatt caggcagcca tggatattgc aaaccacgac attcatgtct
      121 ggctggtgga acgcgaaccg acgatcggag gccacatggg aatgctcgat aaaacgttcc
      181 cgaccaacga ctgttcgatg tgtattcttt ccccgaagat ggccgaggtc ggcagacacc
      241 cgaatattac cctgctgacg ctgtccgagg tggaaaagat cgagggaacc gcaggcgact
      301 ttaccgttac cgtcaccaaa tatccccgct atatccgtga agaggactgt accggctgcg
      361 gcgactgtgt caatatctgc ccggttgagg tgtacaataa gtttgatgca ggtctcggcg
      421 tcagaaaggc gatctacaaa ccgcatgccc aggtggtgcc gaacctggtt gtgaaagaca
      481 atctgcactg tattgagtgc ggactctgtt atgaggtctg cgggaaaaac gctgttctcc
      541 atgttgatga agaccgtgtg aagaccctga ccattcccgc cgcggctgtg gtgattgcca
      601 ccggctacac cctctttgat gcccagaaga agggacagtt cggttatctg cggtatccgg
      661 atgttatcac aagtattgag tttgaacgga tgatcaacgc aggaggtccg acgctcggcg
      721 aagtgcggag actttccacc ggtaagacgc cgaagtctgt cgtgtttgtc cagtgtgtcg
      781 gcagccgtga tgtctcgatt gggcgaaatc tctgttcctg cgtctgctgt atgtatgcac
      841 tcaagaactc gatgctcatc aaagagcatt acccggagac cgaggtcact atattatata
      901 acgatctccg ggcctacggt aagggttacg aggagtatgc ggaacgggcg aaacagatgg
      961 gtgtcaacat cgtccgggcg ttcccgggtg aggtgcagca gaccagaaac ggcctggttc
     1021 ttccccttga agataccgag accggcgagt tcaaaaatct tgaggcagag cttgtcattc
     1081 tctcggtcgg tatggagccg gagcctgata cggtgcgtct tgcaaaatcc ctcggtcttc
     1141 cgctggatca gaacaacttc ctgtcgtccg ctgacatgaa gctggatccc gcgggaacga
     1201 tccgtcccgg tatctacatt gcaggggccg cggttgcacc aaaggatatt cccgactcgg
     1261 tcatctccgg cggagctgcg gcaatgaagg caacaatcga ctcttataca tcggaggtga
     1321 aggagtgacg gacgagacaa agacaatctg gtggaatgat gagaacaact cgatcatgct
     1381 gatcgatcag acaaagctgc cggtcgagtt ctccatcatt gaggtaacga ccgttgaacg
     1441 gctggcggag gcaatccgcc gtcttgaggt tcgcggggca ccggctcttg gtgttgccgg
     1501 agcgttcggt gtggctctgt ctgcggtttc ctgcgtctcg gacgcagagt ttgccgaaac
     1561 tgttgccgcg gacgcggagc ttctgcgtag cacccggccc acggcggtga atctttcctg
     1621 gggtattgac aaagttctcc gctcaatgga gaatctgccg ccggagatgg caaagtttct
     1681 tgcgattacc gcggcaaaga acatcgccgc agaagatgag aagtgctgta tgcttctcgg
     1741 ccacaacggg gcttcgcttc ttccacagat cggaacggtg cttacgcact gcaacgcggg
     1801 ggctcttgcc tgttccacct ggggaacggc tctcggtgtg atccggtcgg cccacaagat
     1861 gggcaagaag atctcggttg tctcctgcga aacacgaccg cttcttcagg gtgcacggct
     1921 gaccgcatgg gaacttgcgc acgataatat tccggtgacc tcgatcatcg actcggaggc
     1981 ggcgtatctg atgcggcagg gaaagatcga ctgtgtggtt gtgggtgccg accgcattac
     2041 ccgcgatgcg gtctttaaca agatcggtac ctacatgcat gcggtctgcg cgaaacatca
     2101 caacattccg ttctacgttg cagcaccaat ctcgaccttc gatgttaatg ccgttgaggc
     2161 ggacattgtg gtagaggagc ggacccgcga tgaggttgcg gcattctggg gcaaaccgac
     2221 cgtgccagcg ggagttccgg tgatcaacta cgcgtttgat gcaacaccgc ttgatcttgt
     2281 tacggccatt atcaccgaga agggtgtgtt ctatccgccg tatgatttct cttcgctcgg
     2341 gcagatgtaa ccaatgtcgg taactctgtc cctttccatc ttcaataacc cgtactggga
     2401 ctcagtagct ctgatgctcg gagatctggc gctcctggtg ttttttgtct ggctgatctt
     2461 tctgatgatc accgtcgggc tgtttgttct ttcggtcaga aagaaacatc tgtactgtcc
     2521 ctggcttctg cggcctgcct atcttctgct gaaaggtgcg gtgcggaccg gctgccggat
     2581 gttcgggatt gatgattccg agatcatcac ggtgatgatc cggctggaga atgatgtgaa
     2641 ccggaatgcg tttgcggcgg tgccggttga ggagcgggta atttttcttc cccactgtct
     2701 gcggtcggcg aagtgtccgg ctcatctgac accccttggg atcaaatgtc tggactgcgg
     2761 taagtgcggt ctgggcatgg cgacgaatgc tctttcggat gccgggtata aggtgttcat
     2821 tattcctggt tcgacctata tcaagcggct gctgaaacgg ttccacccgc gggcgatgat
     2881 cggcgtcggc tgtctgatgg agatcaagca gttccaggag cttgcacgaa agattgatat
     2941 gacggcgatg ggtgttgtga tgacgtcgga cggttgtgtg gagacgacgg ttgagtggga
     3001 g
//
LOCUS       DALTZZ010000225         1548 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_36676,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000225 DALTZZ010000000
VERSION     DALTZZ010000225.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36676"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            59..391
                     /locus_tag="O0X12_04340"
     CDS             59..391
                     /locus_tag="O0X12_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HJK53417.1"
                     /translation="MPESYSSIRSVVLATLEQQLPDLRDTYGIAEIGIFGSVSRGEDT
                     PDSDIDILYSFQPGKATLANLTGLHEHLEQTFGRKIDLVSRKWLSPFIRPSVERDAII
                     CTTGAEAA"
     gene            388..726
                     /locus_tag="O0X12_04345"
     CDS             388..726
                     /locus_tag="O0X12_04345"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HJK53418.1"
                     /translation="MKPSDVGIEGFLRHIYQETSFITTYLSTLTHEEYEQNRVSRYAL
                     VRSLEIIGEAAKHIPETYRSEHPEIPWKLLAGNRDTLIHGYFTIFSVSAICRRREGSS
                     LLSCHCKAIA"
     gene            complement(1106..>1548)
                     /locus_tag="O0X12_04350"
     CDS             complement(1106..>1548)
                     /locus_tag="O0X12_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53419.1"
                     /translation="RGATGSNTFTVKKQLCEDALKKNYVRVVSDKENIADGSEGIILE
                     SMLEGMAKYYAAELPVNIVRGHTENALKGKYNGGTPHFASPSTQTSTTCRTRSPPPVV
                     QDVFKMYNIGSTMKQIVDHMNDLGVTTVRGKSADLNFISGILHN"
BASE COUNT          353 a          422 c          362 g          411 t
ORIGIN      
        1 gaaattatca tatgattcca gtacaaatac ctccagtaca gaatccgggg aatgccgtat
       61 gcctgaatct tactcttcaa tacgatcagt cgtccttgcc actcttgagc agcagctgcc
      121 ggatcttcgt gatacctacg gaatagccga gataggtatc ttcggatccg tcagccgcgg
      181 cgaagacacc ccggattcag atatcgacat tttgtactct ttccagcccg gaaaagccac
      241 ccttgccaat ctcaccggtc ttcatgaaca tcttgagcag acgtttggca gaaagatcga
      301 tcttgtcagc agaaaatggc tcagtccttt tatccgcccc tccgttgagc gtgatgccat
      361 tatctgcaca accggagcag aagccgcatg aaacccagtg atgttggtat tgagggtttt
      421 ctccgtcaca tctatcagga gaccagcttt atcaccacgt atctttccac tctcacgcat
      481 gaggagtacg agcaaaaccg cgtcagcaga tatgcccttg tccgctccct tgagatcatc
      541 ggtgaagcag ccaaacatat ccctgaaaca taccgcagtg aacatccgga gatcccctgg
      601 aaactgcttg caggaaaccg cgataccctg atacacggtt atttcactat tttttctgta
      661 tccgccatct gccgacgcag ggagggcagc tcgttacttt cttgccactg caaggccatt
      721 gcctgatcgg taaaatactc aacaaaggaa tcatccatga tattccgcat gacggaactg
      781 accacggcct gctccaacgg ctctttccga acacctttgt gcttagcggt gcagattttt
      841 ctctttttgc tgttgacaca gcgataatag tggtatcttc tcccttgact gccgcttgtc
      901 ccactttcgc ccaccatcat ggccttacag gtgccgcaga atagtttggt tgtcagcagg
      961 tagtcgtcct cagccttatg acgggttggg gcctttttat ttttcttcat ctgttcctgc
     1021 acacgcgcaa acaggtcggc tgggatcaat gcagggtgca gttaggcacc acaatgtccc
     1081 gatactgata ctcgccgatg tactttcaat tatgcagaat cccagagata aaattcaggt
     1141 ctgccgattt gccacgtact gttgtaacac ccaagtcatt catgtgatcc acaatctgct
     1201 tcatggtgga accgatgtta tacatcttga agacgtcctg cacaacgggg ggcggtgatc
     1261 gggtccggca ggtagtgctt gtctgcgtcg atggcgaagc aaaatgcggg gtgccgccat
     1321 tgtacttgcc cttgagggca ttttctgtgt ggccacggac gatatttacg ggaagctcag
     1381 cggcatagta tttcgccatc ccctctagca tggattctag gatgattccc tcggatccgt
     1441 cggcaatatt ttctttgtcc gaaaccaccc gcacatagtt ctttttcagg gcgtcctcgc
     1501 agagctgctt tttgacggtg aaggtgttgc tgccggtagc gccacgac
//
LOCUS       DALTZZ010000226         1984 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_36787,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000226 DALTZZ010000000
VERSION     DALTZZ010000226.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36787"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="O0X12_04355"
     CDS             complement(<1..260)
                     /locus_tag="O0X12_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HJK53420.1"
                     /translation="MKVLLINGSPHKEGCTFTALSEVAGVLNRRNIGTEIFHIGTKPL
                     AGCIACGKCAETKRCVFHDSVNEVLDRIDEFDAIVVGSPVYYA"
     gene            complement(257..814)
                     /locus_tag="O0X12_04360"
     CDS             complement(257..814)
                     /locus_tag="O0X12_04360"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HJK53421.1"
                     /translation="MSKKILILNGSPRPKGNTVALIDAFVCGAESAGHSVTRFDLQKM
                     DVHPCCGCLGGGKDPRSPCVQKDAMEEVYPVYREADVVVFASPMYYWSVTAQLKTALD
                     RLFAVTECDPEYRTPQKECVLLMPAEGDSEENFAPMVSYYQSLLKNLGWKNRGMVLAG
                     GVLHTGDISGRPILTVAEELGRSLV"
     gene            919..1260
                     /locus_tag="O0X12_04365"
     CDS             919..1260
                     /locus_tag="O0X12_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJK53422.1"
                     /translation="MIKTYIENSNFEDTGFSYTLSLISGKYKMVILYCLMEFNVVRYN
                     ELKRYIKPISHKTLSNCLKELEKDKLIIRTEYPQIPPKVEYRLSAKGQSLMEILDQLC
                     IWGEAHKNLTE"
     gene            complement(1330..1518)
                     /locus_tag="O0X12_04370"
     CDS             complement(1330..1518)
                     /locus_tag="O0X12_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HJK53423.1"
                     /translation="MVEMMTTETAYAILGVPVGSDFATTAKAFRELRDKYNPETNPFR
                     RKEFAEVMAAFEFLQRQF"
     gene            complement(1564..>1984)
                     /locus_tag="O0X12_04375"
     CDS             complement(1564..>1984)
                     /locus_tag="O0X12_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HJK53424.1"
                     /translation="VDSSSSHLAWVSDIEANFGVHVPFPIIADLDMGVASLYGMIQPA
                     MSSTAACRAVFFIDPKGILRAMIYYPLTTGRCVAEILRVIDALQVNDEHHVATPANWK
                     PGDKVIVPAPKTQKEMDEREKITGPDKKTWYLLMKDL"
BASE COUNT          595 a          562 c          391 g          436 t
ORIGIN      
        1 gcatagtaca ccggagaacc cacaacaatc gcatcaaact cgtcaatacg gtccaaaacc
       61 tcattcaccg aatcgtggaa aacacaccgt ttcgtctcgg cacacttccc acaggcaata
      121 catcctgcga gcggtttggt accgatatga aaaatctctg tcccgatgtt gcgacggttc
      181 agtacccccg caacctcaga cagtgccgta aaggtacatc cctccttgtg tggactgccg
      241 ttgatcagca gaaccttcat accagtgacc tcccgagctc ctccgctacc gtgagaatcg
      301 gcctgccgga aatatcacca gtatgcagta caccgcccgc aagcaccata cctctgtttt
      361 tccatccgag attcttcaga agactctgat aataactcac catcggcgca aaattctcct
      421 ctgaatcccc ttccgcaggc atcagcagaa cacactcttt ctgcggcgtc cggtactccg
      481 gatcacactc cgtcaccgca aatagacgat caagcgccgt tttcagctgc gctgttacgc
      541 tccagtagta cattggagat gcaaacacca cgacatccgc ctcacggtac accggataaa
      601 cttcctccat tgcatccttc tgaacacatg gacttctcgg atccttaccg ccgccgagac
      661 atccgcaaca cggatgaaca tccatcttct gcagatcaaa ccgcgtaaca gaatgtcccg
      721 cagactccgc accgcataca aatgcatcaa tcagtgcaac cgtgttaccc tttggacggg
      781 gactaccatt taaaatcaaa atcttcttac tcatacgcga aacctcgaaa ggttatatta
      841 gaaaaataag aatacataca ataaatagag agtgcacaca ttttagtaat atacttacca
      901 tatagagagt gagaaaaaat gattaaaacg tacatagaaa attcgaattt tgaagacacg
      961 gggtttagtt ataccctgtc actgataagc gggaagtata agatggtaat tctgtactgc
     1021 ctcatggagt ttaacgtcgt ccgatacaac gaactcaagc ggtacattaa accaatctca
     1081 cacaaaacac tcagcaactg cctaaaggaa ctggaaaaag acaaattaat cattcgtaca
     1141 gaatatcccc aaatcccccc gaaagtagaa taccgcctat ccgcaaaagg gcagtcgctg
     1201 atggaaatcc tagatcagtt atgcatctgg ggagaggcac acaaaaatct caccgaataa
     1261 tacaggcagg acctgccgga ctcccaaata tacataacct cctttggaaa aaggagacac
     1321 gtagcgatat cagaactgcc gctgcaaaaa ctcaaaggca gccatcactt cggcaaactc
     1381 ttttctgcgg aaaggattcg tctccggatt atacttatca cgaagctcac gaaacgcctt
     1441 tgcagtcgtt gcaaaatccg agccaaccgg aacaccgaga atcgcatacg ccgtctcggt
     1501 cgtcatcatc tcaaccatac tacttacctc aggcggcaaa agagaaaaag tttgaggaag
     1561 gatctacaga tccttcatca gaagatacca ggtcttctta tccggacccg tgatcttctc
     1621 acgctcatcc atctctttct gtgtctttgg cgccggaaca ataaccttgt cccccggttt
     1681 ccagtttgcc ggtgttgcaa catgatgctc atcattcacc tgcaaagcat caatcacccg
     1741 gagaatctct gccacacagc ggcctgttgt cagcggatag tagatcattg cacggagaat
     1801 tcctttcgga tcaatgaaga acaccgcacg gcaggcagca gtcgaactca tcgccggctg
     1861 aatcataccg taaagggaag caactcccat atccagatcc gcaataatcg ggaacggtac
     1921 atgcacgcca aagttcgcct caatgtcact tacccatgca aggtgggaag aggaactgtc
     1981 aacc
//
LOCUS       DALTZZ010000227         2412 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_37297,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000227 DALTZZ010000000
VERSION     DALTZZ010000227.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37297"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /gene="htpX"
                     /locus_tag="O0X12_04380"
     CDS             <1..867
                     /gene="htpX"
                     /locus_tag="O0X12_04380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HJK53425.1"
                     /translation="VMGLIYVVLFSVIAWWLAGTGMFGTSYLYLIIGMAAVMALIQYF
                     LSDKLVLMSTRAKVVSEDEEPKLYAMVKKLADEADLPMPRVAIMPSPVPNAFATGRSP
                     KHAVVAVTQAIRGVLTDEELEAVLAHELAHVKNRDMLTMTIGSFLVSIAAMIINNAFI
                     MALLSSNRDSENGGGIIVFIVAMVVVVLVYIVGTLVTMAISRYREFAADRGSAYITRN
                     PDALISALKKISGKMDTVPPDKKREVSANNAFYIIPAISGESVMELISTHPTLEKRIA
                     NLEKVKQEMQGY"
     gene            939..1009
                     /locus_tag="O0X12_04385"
     tRNA            939..1009
                     /locus_tag="O0X12_04385"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:971..973,aa:Gly,seq:tcc)
     gene            1032..1103
                     /locus_tag="O0X12_04390"
     tRNA            1032..1103
                     /locus_tag="O0X12_04390"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1064..1066,aa:Val,seq:cac)
     gene            complement(1182..1964)
                     /locus_tag="O0X12_04395"
     CDS             complement(1182..1964)
                     /locus_tag="O0X12_04395"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HJK53426.1"
                     /translation="MPVFPGTNCEYDSAKAVSRAGAIPEIVIINNMTAQDTAESVDRV
                     ADAIRRSHAIFIPGGFSGGDEPDGSGKFITAFFRNAKIKDAVHDLLKNRDGLMCGICN
                     GFQALIKLGLVPYGEIVDVDEHSPTLTFNTIGRHQSRIVRTRIASVKSPWFACVNVDD
                     VFAVPISHGEGRFLADEEVLRSLIANGQVATQYVDLADNPTSDIHFNPNNSMLAVEAI
                     TSPDGRVLGKMGHSERVGEGLYKNVPDQFMMQLFESLVKYYQ"
     gene            complement(2145..>2412)
                     /locus_tag="O0X12_04400"
     CDS             complement(2145..>2412)
                     /locus_tag="O0X12_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53427.1"
                     /translation="LPTTDSLLSVFSQVTGLMREGRVLSCWTPTYGGVAEGVLKMGLG
                     NKIGFAYDASVRMDDIFGYRYGSFIRELADDEDVGVLLGRTTDA"
BASE COUNT          631 a          620 c          590 g          571 t
ORIGIN      
        1 gttatgggcc ttatctacgt tgtactcttc tcagtgatcg cgtggtggct ggcaggaacc
       61 ggcatgtttg gaacctccta cctctatctg atcattggaa tggcagcagt aatggcactg
      121 atccagtatt tcctttcaga taaactggta ctgatgtcaa ccagggcgaa ggttgtgtca
      181 gaagatgaag agccaaagct gtatgcaatg gtgaaaaaac tcgctgatga agcagatctc
      241 ccgatgccgc gggtggcaat catgccgtcc ccggtaccaa acgcatttgc aaccggtaga
      301 agcccgaagc atgctgttgt ggcagttacc caggcaatcc gcggagtact caccgatgag
      361 gaactggaag cagttcttgc tcacgagctt gcgcatgtca aaaaccgtga tatgctgacg
      421 atgaccatcg gcagtttcct ggtttccatt gcagcaatga tcatcaacaa tgccttcatt
      481 atggcactcc tcagcagcaa ccgtgacagc gaaaacggag gaggaattat tgtcttcatc
      541 gtggcaatgg tcgtggttgt tctcgtgtac attgtgggga cactcgtaac gatggccatt
      601 tcccggtacc gggagtttgc agcagatcgc ggcagtgctt acatcacccg caatccagat
      661 gcactgatca gcgcactgaa aaagatctcc ggtaaaatgg atacagtacc tcccgacaag
      721 aaacgcgagg tttctgccaa caatgccttc tacatcatcc ccgcaatatc cggcgaatca
      781 gtgatggagt taatctccac gcatccgacc cttgagaaac gtatcgcaaa cctggaaaag
      841 gttaagcagg agatgcaggg atactaatcc ctgattttct tcttctgagt acttcggcag
      901 atttaataac ttcaaagtga aatatatatg acgcaaaggc actgatggtc tagaggtatg
      961 actttggcct tccaagccaa tagcccgggt tcgaatcccg gtcggtgcac tccctttttg
     1021 tgggttttct tggattcgtg gtctagtggt atgacgtcgc cttcacacgg cgaaggtcag
     1081 gagttcgaat ctcctcggat ccactcattt ttagtatatt tattcagaat aacaaagaat
     1141 ttcaaaaaga gatctctgga aaaagagaga agaagagatc atcactgata gtatttgacc
     1201 agtgattcaa agagctgcat catgaactga tccggcacgt ttttgtacag cccctcaccc
     1261 acacgttccg agtgccccat cttaccaagg actcttccgt ccggtgaggt gattgcctca
     1321 accgccagca ttgagttgtt cggattgaag tgaatgtcag atgtgggatt atccgcaaga
     1381 tcgacatact gggttgctac ctgaccgttt gcgatcaggc ttcgaagaac ttcttcatct
     1441 gcaaggaagc gtccttctcc atgcgaaatg ggaacggcaa agacatcatc cacattcacg
     1501 catgcaaacc acggtgactt cacggatgca atgcgtgtcc gcacaatccg cgactgatgg
     1561 cgtccgatgg tgttgaaggt gagggttggc gagtgttcat ccacatccac aatctcacca
     1621 tacggcacaa gacccaactt aatcagcgcc tggaacccgt tgcagatacc acacatcaat
     1681 ccatcgcgat tcttcagaag atcatgtacc gcatccttga tctttgcatt gcggaagaac
     1741 gccgtgatga atttacccga gccatcgggt tcatcaccac cggagaaacc tccgggaatg
     1801 aagattgcat gcgaacggcg gatagcatcc gccacgcgat ctacggactc agcggtgtcc
     1861 tgagcagtca tgttgttgat gataacaatt tcaggaatcg caccggcacg cgagacagct
     1921 ttagcactgt cgtactcgca gtttgtaccc gggaagaccg gaatgagtac acggggatgg
     1981 gcaacacgga cacgggggtt tgcagtgttt cgttcggtat aggagatctt ctcaattaca
     2041 ccatctccct gggagatgtt gcaggcaaat accggttcaa gttttgcctc ataggcatcc
     2101 tgaatctcat cgatcgaaac cgtttcagaa ccgaaacgaa gctcctaggc atcggtagtt
     2161 ctgccaagca gaactcccac gtcctcatca tcggcaagct cacggatgaa tgacccatac
     2221 ctgtagccga aaatatcgtc catcctaacg gatgcgtcat aggcaaaacc gatcttattc
     2281 ccaagaccca tcttcagaac accttccgca accccgccat acgttggagt ccagcaggaa
     2341 agcacccggc cctcgcgcat cagacctgtc acctgtgaaa agacggaaag cagggagtct
     2401 gttgtcggaa ga
//
LOCUS       DALTZZ010000228         2991 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_37826,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000228 DALTZZ010000000
VERSION     DALTZZ010000228.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37826"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="O0X12_04405"
     CDS             <1..490
                     /locus_tag="O0X12_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HJK53428.1"
                     /translation="LDLGDGLMAHGSREKRVAAMTDRTTGAGALALGMMIVLITFAGF
                     ASLPPLWIAAAVLCGEVFGKMVMALFSALGKPFHEGIQSYIYDHSKRHFSVYAVILTL
                     PLFLLPEKIVVAAGLAASLLVFGVLWYLARRCFGGVNGDVTGAGNEITRAAVAVVFAL
                     LL"
     gene            complement(497..805)
                     /locus_tag="O0X12_04410"
     CDS             complement(497..805)
                     /locus_tag="O0X12_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53429.1"
                     /translation="MKTLPLNRKPNGKATVMCQDKEVSVHSTCVFCAHCAGIRVNRRV
                     TPNPYAQALRGSKGGLPMDQQLMEGMMLFNTVIEDANATDIECSDDAGCGFTTLSRRR
                     "
     gene            993..2270
                     /gene="tuf"
                     /locus_tag="O0X12_04415"
     CDS             993..2270
                     /gene="tuf"
                     /locus_tag="O0X12_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor EF-1 subunit
                     alpha"
                     /protein_id="HJK53430.1"
                     /translation="MAAEKPHMNLAVIGHIDHGKSTTVGRILFETGVVQQHILDAYKK
                     EAESKGKGTFEFAWVMDSLKEERERGITIDIAHKKFETPKYSFTVVDCPGHRDFVKNM
                     ITGASQADAAIIVVSGTEGPMEQTKEHVFLSKTLGINQIIVAINKMDAVNYSEEKYNE
                     AKEKMSKLIASVGFKPAETPFIPISAFAGDNIKAPSPNMPWYKGPVLLAALDDFKQPD
                     MPTDKPLRLPIQDVYTISGIGTVPVGRVETGVMKKGMKVSFMPANVEGEVKSIEMHHE
                     EIPQALPGDNVGFNVRGIAKNDVRRGDVCGPVENPPTVAEEFQAQIVVLQHPSVISVG
                     YTPVFHCHTSQTACMFISLDKKLDPRSGQVKEENPAFLKAGDAAICTIQPTRPLVIEK
                     AKELPQLGRFAVRDMGMTVAAGLVLNVKAKQMR"
     gene            2307..2615
                     /gene="rpsJ"
                     /locus_tag="O0X12_04420"
     CDS             2307..2615
                     /gene="rpsJ"
                     /locus_tag="O0X12_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HJK53431.1"
                     /translation="MQKARIRLTGTDYEKVETVCTRIREIAERTGVNLAGPIPLPTKR
                     LIVPIRKSPDGEGTATWDRWQMRVHKRLIDLDADERALRQLMRIQVPKDISIEIVLEN
                     "
     gene            2634..>2991
                     /locus_tag="O0X12_04425"
     CDS             2634..>2991
                     /locus_tag="O0X12_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HJK53432.1"
                     /translation="MPAPTFLSQIQNRVRVEHVFFAILIVAIILRFSFLDLKLFHHDE
                     AIHAWFSYQLLTEGTYIYDPVYHGPFLYYVTAGMFALFGDSDLVGRILPCIFGTALIP
                     LVYCLYRMGYLSGKTAI"
BASE COUNT          681 a          790 c          780 g          740 t
ORIGIN      
        1 gctggatctg ggagacggcc tcatggcgca cggcagccgc gaaaaacggg ttgccgcaat
       61 gaccgatcgg acgacgggcg caggcgcgct tgctctcggt atgatgatcg ttttgatcac
      121 ctttgcaggt tttgcctctc tacctccgct ctggattgcc gcagcagtcc tgtgcggcga
      181 agtgtttggt aaaatggtga tggctctctt ctctgctctg ggaaaaccct tccacgaagg
      241 aatccagagt tacatctatg accactcgaa gcggcatttc tccgtgtatg ctgtgattct
      301 tactctgccg ctttttcttc tgccggaaaa aatagttgtt gcagcagggc ttgctgcatc
      361 gcttctggtg tttggggtgc tgtggtatct tgcgcgccgg tgttttggcg gtgttaacgg
      421 cgacgtcacc ggtgcgggca atgagattac ccgtgcagcc gtggctgtgg tctttgcgct
      481 gctgctgtga tctgaattat cttctgcgcg acagcgtggt aaatccgcag cccgcatcgt
      541 ccgaacactc gatatcggtt gcgtttgcat cctcgatgac ggtattaaag agcatcatac
      601 cctccatgag ctgctgatcc atcggaagtc ctcctttgga tccgcggaga gcctgcgcgt
      661 acgggtttgg cgttacacgg cggtttaccc gtatccctgc acagtgcgcg cagaaaacac
      721 aggtgctgtg aacggatacc tccttgtcct gacacataac ggtcgctttt ccgtttggtt
      781 tcctattaag cggcaacgtc ttcataactc aaaatactgc ctcaaatccg tattagtcta
      841 tcgatatcat gtttccccga cagcgagggt gccacatcct gatctacaga ttattttata
      901 tggttccaca tcgaatctgt tatactcgta ttgagacagg tgacgtgcca gacacgaagc
      961 ctatcaatga gtcaaagtgg agataaatta ctatggcagc agaaaagccc cacatgaacc
     1021 ttgccgttat cggtcacatt gaccacggta agtccaccac tgtcggtcgt atccttttcg
     1081 agaccggtgt cgtacagcag cacattcttg acgcatacaa gaaggaagca gaatccaagg
     1141 gtaagggaac tttcgagttc gcctgggtaa tggactccct caaggaagag cgtgagagag
     1201 gtatcaccat cgatatcgcc cacaagaaat tcgagactcc gaagtactct tttaccgtcg
     1261 tggactgccc tggtcaccgt gacttcgtca agaacatgat caccggtgca tcccaggctg
     1321 atgcagcaat catcgtcgtg tccggaaccg agggtccgat ggagcagacc aaggaacacg
     1381 ttttcctgtc caagactctt ggtatcaacc agatcatcgt tgcaatcaac aagatggacg
     1441 ctgttaacta ctccgaggag aagtacaacg aagcaaagga aaagatgtcc aagctcattg
     1501 catccgttgg attcaagccg gctgagactc cgtttatccc aatcagtgca ttcgcaggcg
     1561 acaacatcaa ggcaccgtca ccgaacatgc cgtggtacaa gggtcctgtc cttcttgcag
     1621 cactcgacga cttcaagcag ccggacatgc cgaccgacaa gccgctcaga cttccgatcc
     1681 aggatgtgta caccatctcc ggtattggaa ctgtgccggt tggccgtgtt gagaccggtg
     1741 tcatgaagaa aggaatgaag gtctccttca tgccggcaaa cgttgagggt gaggttaagt
     1801 ccattgagat gcaccacgag gaaatcccgc aggcactgcc gggtgacaac gttggtttca
     1861 acgtccgtgg tatcgcaaag aacgatgtcc gccgcggtga cgtctgtggt ccggttgaga
     1921 acccgccgac cgttgcagag gagttccagg cacagatcgt tgttctgcag cacccgtccg
     1981 tcatctccgt cggatacact ccggtcttcc actgccacac ctcccagacc gcctgtatgt
     2041 ttatctccct tgacaagaag ctcgacccgc gctccggaca ggttaaggaa gagaacccgg
     2101 cattcctcaa ggctggcgac gcagcaatct gtaccatcca gccgacccgc ccgctcgtta
     2161 tcgagaaggc aaaggaactc ccgcagctcg gacgtttcgc agtccgtgat atgggtatga
     2221 ccgttgcagc aggtcttgtt ctgaacgtca aagcaaagca gatgagataa tctcatctat
     2281 cacgaatttt ttgtggtgat ttgacaatgc agaaagccag aatacgcctt actgggactg
     2341 attatgagaa agtggagacg gtttgtaccc gtatccgaga gattgcagag cgtaccggtg
     2401 taaatctggc aggacccatc ccgttgccga ccaagcgtct tatcgttcca atccggaaga
     2461 gtccggatgg agaaggtacc gcaacgtggg atcgctggca gatgcgcgtt cacaaacgcc
     2521 ttatcgacct tgacgccgat gagcgtgcgc tcagacagct gatgcgtatc caggttccga
     2581 aggatatcag catcgagatt gttctggaga actaaaatcc gaggtgaggg cctatgcccg
     2641 ccccaacctt tttgtcacag attcaaaatc gtgttcgtgt cgaacatgtc tttttcgcta
     2701 ttctgattgt tgccattatt ctgagattct cgtttttgga tctcaaactg tttcatcatg
     2761 acgaagcgat tcacgcatgg ttttcctatc aactgctgac agagggaacc tacatctatg
     2821 atccggtcta tcacggcccg ttcctgtatt acgtcacggc gggaatgttt gcactgtttg
     2881 gtgactcgga tctggtaggc cgtatcctcc cgtgcatctt tggtactgca ctgattccgc
     2941 tggtgtactg tctgtaccgg atgggctatc tttcgggaaa gactgctatc a
//
LOCUS       DALTZZ010000229         2027 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_37837,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000229 DALTZZ010000000
VERSION     DALTZZ010000229.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37837"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="O0X12_04430"
     CDS             <1..206
                     /locus_tag="O0X12_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase"
                     /protein_id="HJK53433.1"
                     /translation="VLASGANFVLFDVAPMTSNEAMEAFAKAGVLVRSCASFPGLGET
                     FVRVSVGDVWENERFLAAVKQLG"
     gene            203..712
                     /locus_tag="O0X12_04435"
     CDS             203..712
                     /locus_tag="O0X12_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HJK53434.1"
                     /translation="MIGITGTPGTGKSAAADELRRRGLPVTDLKTTVSPYIVEHDLVR
                     DADEVDVEAWADAFPYQDGYVEGAIAHFLACDKIIVLRCRPDVLKDRLSRRGYSAGKV
                     AENCQAEALDVILSETADMFASEQVYEIDTTNRDVISVADLIVSFAADEIPASFGTID
                     WSEFLDPML"
     gene            709..1344
                     /locus_tag="O0X12_04440"
     CDS             709..1344
                     /locus_tag="O0X12_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HJK53435.1"
                     /translation="MTLDSYRSKVAFIVTPFAKAVAALHLTPNACTVLALLAAAASGI
                     FFGLGETLIAVVLVLVSAFFDAIDGAVARLTGIASPAGDYLDHVFDRYADIFIITGIF
                     AWGTSAWTCEVPAWAIGVFAMTGVLMSSYLGTQAQAVGLKRNYGGILGRADRLVLLMV
                     FGAVEVVFPSPLFLGLPALGWLLVIFGVFGHVTAVQRFVSGWRELNAPRKE"
     gene            1380..1787
                     /locus_tag="O0X12_04445"
     CDS             1380..1787
                     /locus_tag="O0X12_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HJK53436.1"
                     /translation="MPVSVQTKNQITDLLERYQSAYNDHNADGLAVLLAEDVVMYGCR
                     TEEVIIGRESFLSAMRKNFERYKTGGIRFADAEIKSEGVIAWVSASCMLLSTAGSGAV
                     HETPCRFTAVLRGTGHAWEFAQIHMSLGYPYHA"
     gene            complement(1801..>2027)
                     /locus_tag="O0X12_04450"
     CDS             complement(1801..>2027)
                     /locus_tag="O0X12_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HJK53437.1"
                     /translation="REHPGITKQELAGELRISPSSCNWYLRRFRDDGVIMIIFDGVHN
                     HYRLTSEAEAAYKTILTDQAETEDPDITVS"
BASE COUNT          343 a          423 c          672 g          589 t
ORIGIN      
        1 cggttcttgc gagcggtgca aacttcgtgc tgtttgatgt tgctccgatg acgagcaatg
       61 aggcgatgga ggcgtttgca aaagcaggcg ttcttgtccg ttcgtgtgcg agcttcccgg
      121 gtcttgggga gacgtttgtc cgtgtctctg tcggggatgt gtgggagaat gaacggttcc
      181 ttgcggcggt gaagcagctg ggatgatcgg gattaccgga acgccgggta cgggtaagtc
      241 tgcggcggcg gatgagctgc gcaggcgtgg tcttccggtg accgatctga agacaaccgt
      301 ttctccctac attgtggagc atgatttggt ccgcgatgcg gatgaggtgg atgttgaagc
      361 gtgggctgac gcttttccct accaggatgg gtatgtggaa ggggcgattg cccattttct
      421 tgcgtgtgat aagatcattg ttctccggtg caggccggat gtgctgaagg acaggctttc
      481 ccggcgcgga tactctgctg ggaaggttgc ggagaactgt caggcggagg cgctggatgt
      541 gattttgtcg gagactgcgg acatgttcgc ttcggaacaa gtatatgaga tagatacgac
      601 aaatagagat gtaatttctg ttgccgatct gattgtctcg tttgcggcgg atgagatccc
      661 tgcttcgttt gggacaatcg actggtcaga gtttctggac ccgatgttat gacgcttgat
      721 tcgtaccgtt cgaaagttgc ctttattgtg acgccgtttg ccaaggctgt tgccgcactg
      781 catctgacgc cgaatgcctg tactgtgctg gcacttcttg cggctgcggc ttcaggtatc
      841 tttttcggtc ttggggagac actgattgcg gttgttcttg tgctggttag tgcgtttttt
      901 gatgccattg atggtgcagt ggcacgactg acaggtattg caagtcctgc gggtgattat
      961 ctggatcacg tgtttgaccg gtatgcggat attttcatta ttacgggtat tttcgcatgg
     1021 ggtacgtctg cgtggacctg tgaggttccg gcatgggcga tcggtgtgtt tgctatgacg
     1081 ggtgttctga tgtcttcgta tcttggaaca caggctcagg cggttggtct gaagcggaat
     1141 tacggcggga tcctcggcag ggccgatcgg ctggtgcttt tgatggtgtt tggtgcggtt
     1201 gaggttgtgt ttccgtctcc gctgttcctg ggtcttccgg cgctcgggtg gctgctggtg
     1261 atctttggtg tgttcggtca tgtgacggcg gttcagcggt ttgtgagcgg ctggcgtgag
     1321 ctgaatgctc cgaggaagga gtaacttttt tttggttttt gcggtaatat ctaacaggta
     1381 tgcctgtttc tgtacaaacg aaaaatcaga tcacggatct tctcgagcgg tatcagtccg
     1441 cctataatga tcataatgct gatggtcttg ccgttcttct tgcagaagat gttgttatgt
     1501 acggctgccg gaccgaggag gttatcattg ggcgggagag ttttctttct gcgatgagga
     1561 aaaattttga gcgttacaag acgggaggca tccggtttgc ggatgcggag atcaagagcg
     1621 agggtgtgat cgcctgggtt tcggcttctt gtatgctgct gagtactgcg gggtcaggtg
     1681 cggtgcatga gacgccctgc cggtttacgg cggttctccg cgggaccggt catgcgtggg
     1741 agtttgcaca gattcatatg tcgctcggtt atccatacca cgcatgagag ggatctcctg
     1801 tcatgataca gtgatgtcag ggtcttcggt ttccgcttga tcggtgagga tcgttttgta
     1861 tgcggcttct gcttcactgg tcagccggta gtggttgtgt actccgtcaa agatgatcat
     1921 gattacgccg tcgtcacgaa accgccggag ataccagttg caggaggacg ggcttatccg
     1981 gagttctccg gcgagctcct gtttggtgat ccccgggtgt tcgcgga
//
LOCUS       DALTZZ010000230         3778 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_38045,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000230 DALTZZ010000000
VERSION     DALTZZ010000230.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3778)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3778)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3778
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38045"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="O0X12_04455"
     CDS             <1..666
                     /locus_tag="O0X12_04455"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HJK53438.1"
                     /translation="VHDLVRCSICPLQVYLARSDPEEFQEPVNYSIAKQISLHFGDEL
                     KPDEIWDELETVLSVAGAEEYEQTLAMIDACQKTVWRPAVDTDVFVSSQKYGISGKVD
                     RLFDDGFAIVRSSKAPSSGIFAVDRLRLTAYWLCLYEEHKRSFDCSVEYLGSGTVRNL
                     ASPTPADRRAFLAALHSAESVFQGEVPRPRQGHHCLSCIFHERCDTVLRPKSLFERLN
                     LVR"
     gene            723..1373
                     /locus_tag="O0X12_04460"
     CDS             723..1373
                     /locus_tag="O0X12_04460"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HJK53439.1"
                     /translation="MTSVLSEDVLEKIFNTGTDEVPLHHLTFPHHSESEIENVLNILA
                     ERGFVCRGNNTVRLTGKGKEKAAQVARKHAVLETFLKDVLGKQPDAASKEACILEHNI
                     SSETIERLDNFLDQRKPEPVPSETTGFINHNNLTRRNPIVPLSECPEGSLLHVSMIRC
                     FAKHNRLIDLGVIPGELITLRRKLVNNAVVITVKGCDIALSPEVSANIMVERCETP"
     gene            1370..3238
                     /gene="feoB"
                     /locus_tag="O0X12_04465"
     CDS             1370..3238
                     /gene="feoB"
                     /locus_tag="O0X12_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HJK53440.1"
                     /translation="MMPRAVLIGNPSVGKSLIFNQLTGLGVEVSNYPGTTVGVMHGVV
                     RYDDAEFSLVDLPGIYSLSGDSIEEKMVREYLLAEELDLLIAVLDAKHLERNLYLLLQ
                     VAEIKKPLLVILNMMDEAEAAGKIIDTVDLSRKLGVPVIGSVATSGKNLDQIARFVLA
                     NDVPVPTLVVPYDHHIEAAVHSLQKYYGISQSEALWALENVSVSTITPEIHENALVIA
                     EEIERRHMMSPEQIIAANRHNLAKQLAASVTSTAPPRRRRDFDALLTRGFPGLPILAL
                     IMVSILLIVFTLGGILEETIVSLMTTYISDPFHALDLDPFVDTLGGAVILALMSGLGI
                     AFPYVFLFYLFISVLEDTGYLTRAAFLADRGMHKLGLHGQGLIPMVLSFGCSVPAIMS
                     TRLLPTRRERVIASVLVTMLPCSARTVVISGIVASFIGFGAALSIYAIVFVLVFVVGC
                     ILSRITAGEQYGMILEMAELRRPIASHVVRKAWMRVREFIYIAMPLLLVSSIFLGLFE
                     YYGLVAMFEEFIDPISTAVLGLPGFAFTALMFGILRKEMAFETLAVMAGTTDLAAVLT
                     SGQLYIFALVCALFIPCISTIAVLMRETGVRYAAMITVFTLCLGIGLGALTHLILL"
     gene            3282..>3778
                     /gene="dph5"
                     /locus_tag="O0X12_04470"
     CDS             3282..>3778
                     /gene="dph5"
                     /locus_tag="O0X12_04470"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HJK53441.1"
                     /translation="MLTFIGLGLCDEYDVSVRGLEAIRQADAVFLEVYTSVLTGTTIP
                     RLEEYYGKKITLLYREDVELHPEPILDAAVNGDAVFLTAGDSMVATTHADIRIRAAER
                     GIPTRIIHGASITTAVCGLSGLQNYRFGKSVSVPFPYGKWFPMTPIEVISANLSQNLH
                     TLVFL"
BASE COUNT          845 a          874 c          944 g         1115 t
ORIGIN      
        1 gtacacgatc ttgtccgttg cagtatctgt ccattacagg tgtatttggc ccgttctgat
       61 ccggaagagt tccaggagcc ggtaaactac agcatcgcaa aacagatatc cctacatttc
      121 ggggatgaac tgaagcctga tgagatatgg gatgaacttg aaaccgtttt gtctgttgca
      181 ggagctgaag agtatgaaca gactttggca atgattgatg catgccaaaa aactgtttgg
      241 cgtccagccg ttgacactga tgtctttgtg tcctcacaga agtatggtat ttccggaaaa
      301 gtggataggc tgtttgatga tggttttgca atcgttcgga gtagtaaagc acccagctcc
      361 ggtattttcg cagtagatcg gctccgcctg actgcatact ggctttgtct ctatgaggag
      421 cacaaaagat cgtttgactg ttccgtggag tatcttggtt ctggaaccgt acggaatctt
      481 gcgtctccta cccctgctga tcgccgtgcg tttcttgcag cacttcacag tgctgaatct
      541 gtttttcaag gcgaagtacc gcgtccccgt caagggcacc actgtctttc ctgtatattt
      601 catgaacggt gtgacacagt tttacgcccg aaatctctgt ttgagcgtct taatctcgta
      661 agatgacgat atgtagatat ttgttcagaa cagaaatggt aggtgcaatt gtgtgattct
      721 gcatgacctc ggtactctct gaagacgttt tggaaaaaat attcaatact ggcactgatg
      781 aggtgcctct tcatcatctt acttttcccc atcactctga gtctgagatt gaaaatgtcc
      841 tgaacatcct tgctgaacga ggttttgtgt gccgcgggaa caacacggtc aggcttactg
      901 ggaaaggaaa ggaaaaagct gcgcaggttg cacgcaagca cgcagttctt gaaacctttc
      961 tgaaggatgt tttgggaaaa caaccggatg ctgcgtctaa agaagcctgt attctggaac
     1021 acaatatctc atcagaaaca atcgaacggc tcgacaattt cctggatcaa cgtaaaccgg
     1081 aaccagttcc ttcggagacg accgggttca ttaatcacaa taatcttacc cgcagaaatc
     1141 cgattgttcc gctttccgaa tgcccggagg gttctcttct gcacgtttcc atgattaggt
     1201 gttttgcaaa acataacaga cttattgatc ttggggtgat tcctggtgag ctgattaccc
     1261 tgcggcgtaa acttgtcaac aacgctgtgg ttatcactgt gaaaggctgt gatattgcat
     1321 taagtccgga ggtatccgcc aacataatgg ttgaacggtg tgaaacacca tgatgccgcg
     1381 tgcagtactt attgggaacc cgagtgttgg aaaatcgctt atctttaatc aactgaccgg
     1441 tcttggcgtt gaggtgagta actatccggg aacaacagtt ggggtcatgc acggtgttgt
     1501 ccggtatgat gatgccgagt tctcccttgt tgatctcccg ggtatttact ctctgtcagg
     1561 tgactctatc gaggagaaga tggtccgcga gtacctgctg gcagaagaac tggatcttct
     1621 gattgctgta ctggatgcaa aacaccttga gcgcaatctc tatcttctct tgcaggttgc
     1681 cgaaatcaaa aagccgcttc ttgtaattct taacatgatg gacgaggctg aggctgccgg
     1741 aaagatcatt gacaccgttg atctttcccg gaaacttggt gtcccggtca ttggttctgt
     1801 tgcaacaagc ggtaagaatc tggatcagat tgcccgattt gttcttgcga atgatgttcc
     1861 agtcccaacc cttgttgtac cctatgatca ccacatcgaa gcggcagttc acagtctcca
     1921 aaaatactac ggcatcagtc agtctgaggc gctgtgggca ttggaaaacg tttcagtgag
     1981 cacaatcacg ccagaaattc atgagaatgc tctggtgatt gcagaggaaa ttgagcgcag
     2041 gcacatgatg tctccggagc agattatcgc tgcaaaccgg cataacctcg caaagcagct
     2101 tgccgcgtct gttactagta ctgctccccc gcgccgcaga cgtgattttg acgccctcct
     2161 tacacgcggt tttccgggac tgccaatcct tgccctcatc atggtctcga ttcttctgat
     2221 cgtgtttact cttggaggca ttttggagga gacaatcgtt tctttgatga caacgtatat
     2281 ctctgatccg ttccacgctc ttgatcttga tccgtttgtg gatacgcttg gtggtgcagt
     2341 tattcttgcc ctgatgtctg gtcttggcat tgcttttcca tatgtatttc tcttctatct
     2401 gttcatctcg gtactggagg atactggata tttaaccagg gcagcatttc tggcagatcg
     2461 gggtatgcac aaacttggtc tccatggtca gggcctgatt cccatggttc tgtcctttgg
     2521 gtgtagtgta cctgctatca tgagtacgcg actgcttccg acccgtcgtg agcgagtgat
     2581 tgcatcagta ctggttacca tgctcccatg ttcggcacgg actgttgtca tctccggaat
     2641 agttgcatct tttatcggat ttggcgcggc gctttccatt tatgcgatcg tgtttgttct
     2701 tgtctttgtt gtagggtgta ttctctctcg gattaccgcg ggtgagcagt atggaatgat
     2761 tcttgagatg gctgaacttc gccgtcccat tgcttctcat gttgtacgca aagcgtggat
     2821 gcgggtgcgt gagttcatct atattgcaat gccgctcctg ctggtgagca gtattttcct
     2881 cggtctcttt gaatattacg gccttgttgc aatgtttgag gagttcatcg atccgatttc
     2941 aacagcggtt cttggtcttc cgggatttgc gtttactgca ctgatgtttg gtatccttcg
     3001 caaagagatg gcatttgaga cacttgcagt aatggcggga acgacggatc ttgcagcggt
     3061 tttgacgagc ggccagctct acatctttgc gctggtatgc gctttgttta ttccctgcat
     3121 ttccaccatc gcagttctca tgcgtgagac cggtgtgcgg tatgcagcga tgattaccgt
     3181 ctttactttg tgtctcggca ttggtcttgg ggcactgacg catctgattt tgttgtaatg
     3241 agcaagaact cattacattc caaaccctac agaatacata catgctgaca tttatcggac
     3301 ttggactctg cgatgaatat gatgtctccg tacgaggact ggaagcaatc cgtcaggctg
     3361 atgcggtatt ccttgaggtg tacacctctg tattaacagg gaccaccatt ccgcggcttg
     3421 aggagtatta cggaaaaaag atcaccctgc tgtaccgtga ggatgtggaa ctccacccag
     3481 agccgattct tgatgcggca gtaaatggtg atgcagtatt tttaaccgct ggagattcga
     3541 tggttgccac aacgcatgct gatatccgta ttcgtgcggc agaacggggt atcccgactc
     3601 gcattattca tggtgcatcc attacaactg ctgtatgcgg tctgtccggc ctgcaaaact
     3661 accggtttgg caagtctgtt tcggttccgt ttccttatgg caaatggttc ccgatgacac
     3721 cgattgaggt catctctgca aatctctcac aaaatctgca caccttggtg ttccttga
//
LOCUS       DALTZZ010000231         1623 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_38141,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000231 DALTZZ010000000
VERSION     DALTZZ010000231.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38141"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /gene="pyrF"
                     /locus_tag="O0X12_04475"
     CDS             complement(<1..387)
                     /gene="pyrF"
                     /locus_tag="O0X12_04475"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HJK53442.1"
                     /translation="MADLLLALDVKGKSEAVRVANACTGEVDAIKIGYPLVLSTGLGI
                     VKDLAKANIPIIADFKVADIPNTNSLICDEVFGAGCSGIITHAFCGHDSLAACVDSAH
                     DHGGVCFVGCEMSHPGALEFLTGENAE"
     gene            complement(387..1325)
                     /locus_tag="O0X12_04480"
     CDS             complement(387..1325)
                     /locus_tag="O0X12_04480"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HJK53443.1"
                     /translation="MHKHCNDPVKQFIPRAGMTVNELVLEMGGAGAYNGGSLYHAVDI
                     YEKMLSDPDMTKFFGLSGAMVPAGMGGIVSTLIEKGHIDILTSTGANLTHDLIEAIGC
                     HHYHGQAECDDLELRKEEINRIYDVFLPNEAYEELESFIQDVYSSLPEQPITISKLLR
                     TIGEQLDTGILATAAKHDVPIYCPAFQDSVLGLQYWMYSQFHKKTILDAIGDMHDLLN
                     TAFSVKKAGAVLVGGGVPKNFTLQTMLMTENAFTYVVQLTGDRPDLGGLSGATLDEAK
                     SWGKIGEGGIGVTVYGDATITLPILTAATLERLERK"
BASE COUNT          395 a          439 c          381 g          408 t
ORIGIN      
        1 ctctgcattt tcgccggtaa gaaactccag cgcaccgggg tgactcatct cgcagcccac
       61 aaaacagacg ccgccgtgat cgtgtgcaga gtcgacacag gctgcaagag aatcatgtcc
      121 gcagaatgca tgcgtgatga ttccggaaca tcccgcacca aacacctcat cacagatgag
      181 tgagtttgtg tttggaatat cggcaacctt gaagtctgca atgattggaa tgttggcctt
      241 tgcaagatcc ttcacaatcc ccaggccggt tgaaagaacc agcggatacc cgatcttgat
      301 tgcatccacc tctccagtac aggcatttgc cacccggaca gcttcgctct ttcccttcac
      361 atccagagca agaagaaggt ctgccattat ttccgctcca gccgctcaag cgttgcggca
      421 gtcagaattg gcagtgtgat cgttgcatcg ccgtacacag ttacaccgat accgccttcc
      481 ccaatctttc cccaggactt tgcctcatcc agtgttgcac cggaaaggcc accaagatca
      541 ggacggtcac cggtcagctg cacaacatac gtgaatgcat tttccgtcat cagcatcgtc
      601 tgcagggtga agttctttgg aacgccgccg ccgaccagaa ccgcacccgc ctttttcacc
      661 gaaaatgctg tatttaacag atcgtgcatg tcgccgattg catccaggat tgttttcttg
      721 tggaactggg agtacatcca gtactgcaga ccaagaaccg agtcctgaaa cgctgggcag
      781 tagatcggca catcgtgttt tgccgctgtt gcaagaattc ccgtgtcaag ttgctcgccg
      841 atggtgcgca gaagtttact gatggttatc ggctgttccg gaaggctgga gtacacatcc
      901 tgaataaacg actccagttc ttcataggct tcgttgggga ggaatacatc atagattcgg
      961 ttaatctcct cctttctcag ttcaagatca tcacattccg cctgtccgtg atagtggtgg
     1021 caaccgatcg cctcaattaa atcatgtgtg aggtttgcac ctgtcgaagt gagaatatcg
     1081 atatgtcctt tctcgattaa tgtggagaca atacctccca ttcctgcagg aaccatggct
     1141 ccggacagac cgaagaactt cgtcatatcc ggatcggaca gcattttctc gtaaatatca
     1201 accgcatggt agaggctgcc gccattgtat gctcctgcac ctcccatttc cagaacgagt
     1261 tcgttcaccg tcatacctgc gcggggaatg aactgtttta ccggatcatt acagtgtttg
     1321 tgcatttatt gtacctgacc tgaagtaact accatagagt taggcgagat acagcaataa
     1381 gatattggaa aacagatttg actgtggtga gggcagcagt atccacactg ctttgtactc
     1441 cacatccccc acctaactat actattatgc gttagcaact tttaatcttg ccgtctctcc
     1501 ccttgagaaa acccagtttg cggaggtcgt ttatgactcc tgagaggtag tctgcggcaa
     1561 gatcaaagga gatcacatcc tcggtttcgc cgagcgggaa ggccggagct tccaaggcct
     1621 caa
//
LOCUS       DALTZZ010000232         2788 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_38207,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000232 DALTZZ010000000
VERSION     DALTZZ010000232.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38207"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="O0X12_04485"
     CDS             <1..425
                     /locus_tag="O0X12_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833625.1"
                     /note="Sliding clamp subunit. Responsible for tethering
                     the catalytic subunit of DNA polymerase to DNA during
                     high-speed replication. Proliferating cell nuclear antigen
                     homolog; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase sliding clamp"
                     /protein_id="HJK53444.1"
                     /translation="ITLLDTKTIRKDPNAPNLSLPATFEISGSMFYDAIKAAAMVSDK
                     ISLSVNPETQIFTMNAEGDSDRIKRELAGDDVHYLSCAAARSLFSLDYLKDMGKSISR
                     ADKVQIRLGNDHPVQFSFVYADGKGKIGYLLAPRIEAD"
     gene            428..1483
                     /locus_tag="O0X12_04490"
     CDS             428..1483
                     /locus_tag="O0X12_04490"
                     /inference="COORDINATES: protein motif:HMM:NF016030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase large subunit PriL"
                     /protein_id="HJK53445.1"
                     /translation="MRLAVEPRDLTHYPFLKESQEFLAARGIKMNDLAASALGRRYLD
                     AAVERVACAINGKEIYPESSDAVADVATYVLARVLVSCIKDRNLIERLARMEAKRVYF
                     YIQEETNEKLKSHVCETLGITMGIGKMPVIKYVELASTVREPKWRLINRDVDGGMVTI
                     SEDELDVLLRERIRKILVSQLPLTIPPSMEHEFVPWCEKITAALQERTLAEFGAIDES
                     AYPPCIQALIAGAAAGVNLSHSGRFAMTAFLNNIGMTPNQVAGIFARSPDFNPDMTMY
                     QVDHITTHEYTTPACQTMLTHGICVNRDSLCDKIAHPLNYYRAKKKLLERKRLRQEAQ
                     ALADAKKTVSEKPEKQL"
     gene            1599..>2788
                     /locus_tag="O0X12_04495"
     CDS             1599..>2788
                     /locus_tag="O0X12_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HJK53446.1"
                     /translation="MLRYGAERAGDDRPALAYYGNHISYGQLLADVHACAAGLRAHGV
                     KRGDFVTIFLPNIPQCVVAVYAVNLIGAVCNMVHPLSTTGETGYAVDLTESKYVLTCE
                     INEAVCSGMDVEIIRCRTPSYFPKTPKGYLMGWMYRRAVRKCLNGENVRRITEWDDLL
                     AEGRAVVAASGIPEMVGSPDDTAVIMYTGGTTGTAKGVMLSNFAVNTVAVQLVVGIGG
                     EDICVGDGFLAILPMFHAFGLAVTAHTPLSAGLKVVLLPRFNAKECAHAILSEKTAYI
                     AGVPAMYERMYSEFEGKDLSFIKLIVAGGDSVSGSLINRYNQLLRQGNCMVKFRPGYG
                     LTEACSVCALTEDGYRTVPTGCVGIPLMGNKVCTVEPGTINVLPDGEEGELCLFSGAV
                     MTGY"
BASE COUNT          658 a          695 c          746 g          689 t
ORIGIN      
        1 ccattacgct gcttgacacc aagaccatca gaaaggatcc gaacgccccg aacctttccc
       61 tccctgcaac atttgagatc tctgggtcga tgttctacga tgctatcaag gcagcagcca
      121 tggtttctga taagatctcc ttgtctgtta acccggagac ccagatattc accatgaatg
      181 cggagggtga cagcgacaga atcaaacgcg aactcgccgg tgacgatgtc cactatctct
      241 cctgtgctgc ggcacgttcc ctgttctccc ttgactatct gaaagacatg ggtaaatcaa
      301 tcagcagagc agacaaagtc cagatccggc ttggcaacga ccacccggtg cagttttcct
      361 ttgtctatgc tgacggcaaa ggtaagattg gctatctgct tgccccgaga attgaggcag
      421 actaaccatg cgtctcgccg ttgagccgcg cgatctaacc cactatcctt ttttaaaaga
      481 gtcccaggag tttcttgccg cacgcggcat taaaatgaat gatcttgctg catcagccct
      541 tgggcgacgg tatctcgatg cagcggttga gcgggttgcc tgtgctatca acggaaaaga
      601 gatctatcct gaatcatcag atgccgttgc cgatgttgca acgtacgtgc ttgcacgggt
      661 tcttgtttcc tgcatcaaag accgtaatct tattgaacgg ctcgcgcgaa tggaggcaaa
      721 acgggtgtac ttctacatcc aggaagagac gaatgaaaaa ctcaagagtc atgtctgtga
      781 gactcttggt attaccatgg gtattgggaa gatgccggtg atcaaatatg tggaacttgc
      841 ctcgaccgtc cgcgagccga agtggcgtct tatcaaccgt gatgtggatg gcgggatggt
      901 tacaatatcc gaggatgaac tggatgtcct gctgcgggaa cgcatcagaa aaatccttgt
      961 gtcgcagctc cctctcacca ttcccccgtc gatggagcac gagtttgtgc cctggtgcga
     1021 aaagattacc gctgctttgc aggagagaac acttgcagag tttggggcta tcgatgagtc
     1081 agcatatcct ccgtgtattc aggcactgat tgctggggct gctgccggtg ttaacctttc
     1141 ccactcggga aggtttgcaa tgacagcgtt tctgaacaac atcggcatga ctccgaacca
     1201 ggttgcggga atatttgccc ggagtcctga ttttaatccc gatatgacca tgtatcaggt
     1261 ggatcacatt accacgcacg agtacacgac gcctgcctgc cagaccatgc tgacacatgg
     1321 tatctgcgtg aatcgtgatt ctctgtgcga caaaattgcc catcccttaa actactatcg
     1381 tgcaaagaag aaacttctgg aaagaaaacg tctccggcag gaggcacagg cattggctga
     1441 tgcaaaaaaa actgtttcgg aaaagccgga gaaacagctg taatcccgta aatatttata
     1501 gacatcgtcg atacatcata tgagtaaact gtgtggtgtg gaggaacaac gtgccagtag
     1561 taattcatcc tgactcagcg aaacaaaccc tgtattctat gctcagatac ggagcagagc
     1621 gagcaggaga tgaccgccca gctcttgcct attatgggaa ccacatcagt tacggacagc
     1681 ttcttgctga tgttcatgcc tgcgctgctg gtctccgtgc ccacggggtt aaacgtggtg
     1741 attttgttac catctttctt cctaacattc cgcagtgcgt tgttgccgtc tatgcggtca
     1801 atcttatcgg cgcggtctgc aacatggttc acccgctgtc aaccaccggt gagactggat
     1861 atgccgttga tctgaccgaa agcaagtatg tgctgacctg tgagatcaac gaagcggtct
     1921 gttcaggaat ggatgtcgag attatccggt gcagaacccc ctcctacttc cccaaaaccc
     1981 cgaaaggcta tcttatgggg tggatgtacc gccgtgcagt ccggaagtgc ctcaacggtg
     2041 aaaacgtccg cagaataacc gagtgggatg atcttctcgc agaggggcgt gccgtggttg
     2101 ctgcatccgg tattcctgag atggtgggat cacctgacga caccgcagtt attatgtaca
     2161 caggcggaac gaccgggaca gcaaaaggtg tgatgctctc taactttgcg gttaataccg
     2221 tcgctgttca gcttgttgtc ggtatcggtg gtgaggatat ttgtgtgggt gacggattcc
     2281 tggccattct cccgatgttt cacgcattcg gtcttgccgt tactgcccat acaccgcttt
     2341 ctgccggtct gaaggttgtt cttctgccga ggtttaatgc aaaggagtgt gcgcacgcaa
     2401 ttctctcgga aaagaccgca tatattgcag gggttcccgc aatgtacgag cggatgtaca
     2461 gtgagtttga aggaaaggat ctctcgttca tcaaactgat cgttgctggc ggcgacagtg
     2521 tcagcggcag tctcatcaac cggtacaacc agcttctccg tcaggggaac tgtatggtga
     2581 agttccgtcc cgggtacggg ctgaccgagg catgcagtgt ctgtgcactg acagaggatg
     2641 gataccgtac tgttcccacc ggttgtgtcg gtattccgct gatgggaaac aaagtctgta
     2701 cggttgagcc gggaacaatc aatgttctgc ccgacggaga ggagggggag ctttgcctgt
     2761 tcagcggtgc agtaatgacc gggtacta
//
LOCUS       DALTZZ010000233         1513 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_38488,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000233 DALTZZ010000000
VERSION     DALTZZ010000233.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38488"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="O0X12_04500"
     CDS             complement(<1..368)
                     /locus_tag="O0X12_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HJK53447.1"
                     /translation="MKVFVKGTTYLAEPGSYFKDSVKIDGDFIVPPKTQFWKDLVVSG
                     HLSLGVGSRVGGNITCNGAVIGRGCLVEGELDAGSEQLTVCDGARVLVIRSEGNVLLR
                     PGIVSDEVHGENILVMGKIH"
     gene            complement(436..1296)
                     /locus_tag="O0X12_04505"
     CDS             complement(436..1296)
                     /locus_tag="O0X12_04505"
                     /EC_number="2.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HJK53448.1"
                     /translation="MSLSSYVTIIRPANAVVSGITAIIAYLIASGTNPLSAFLLFFVV
                     TVICGAGNVLNDYFDREIDAINRPDRPIPSGAVTPKNAAMWAGVLFVLGILASFATNL
                     WCLAIAVFNSVLLIAYAAKLKKMPLFGNLSVAYLSGSVFLFGGVLVGPESFAVTLPLF
                     AITFFGTLAREILKAAEDIEGDVAGGAKTLPMILGVQKSGYLAVILILCAIAASVLPY
                     SRWGVVYLVLIAVIDIFILYSAVKSVRCKTSAELIASKSTSLIKYGMFAALFLFLVME
                     VWYGILAPVL"
BASE COUNT          449 a          403 c          350 g          311 t
ORIGIN      
        1 cagtgaatct ttcccatcac cagaatattt tcaccatgca cttcatcaga aacaatcccc
       61 ggacgaagca gaacatttcc ctcggaacga ataaccagca cacgtgcacc gtcacaaacc
      121 gtcagctgtt ctgatccggc gtccaactca ccttccacaa gacagccacg accaataacc
      181 gcaccattgc aggtaatgtt gccgccgacc cgtgaaccaa caccaaggga aagatgaccg
      241 ctgaccacaa ggtccttcca gaactgggtt ttggggggca caatgaaatc tccgtcaatt
      301 ttaactgagt ccttgaagta ggatcccggt tctgccaagt aggtcgtgcc ctttacgaaa
      361 actttcattg gatacccctg ttacgtataa taaccttctt tctcacggta ttaatatgtt
      421 gtccatcacg gattttcaca ggaccggtgc aagaatgccg taccacactt ccatgacaag
      481 gaacaggaaa agtgccgcaa acataccgta cttaatcaga ctcgtggact ttgaagcaat
      541 cagctccgca gaggttttgc agcggacgga tttaaccgca gagtagagaa tgaagatgtc
      601 aattactgcg atcaaaacaa gatacacaac gccccagcgt gagtacggca gaacacttgc
      661 tgcaattgca caaagaatga gaattaccgc aaggtacccg cttttctgta caccaagaat
      721 cattggaaga gtctttgctc ctccggcaac atcgccctca atgtcctcgg cagccttgag
      781 aatttcgcgg gcaagagttc caaagaacgt aatggcaaac agcggcaggg tcactgcaaa
      841 cgactccggc cccacaagaa caccgccgaa gagaaaaacg cttccggaaa gatatgcaac
      901 cgaaaggtta ccaaacagcg gcatcttttt cagctttgca gcataggcta tcagcagaac
      961 tgagttgaac acggcaattg ccagacacca gagattggtg gcaaaacttg caagaatccc
     1021 aagaacaaac agaacaccag cccacatcgc cgcattcttg ggcgtaaccg caccggacgg
     1081 aatcggtctg tcgggacggt tgatggcatc gatctctctg tcgaagtagt cgttgagcac
     1141 atttcccgca ccgcagatta cggttacgac aaagaacagc agaaacgcgg acaacgggtt
     1201 cgttcccgat gcgatcagat aggcaatgat agcggtgata ccggaaacaa ccgcgtttgc
     1261 cggacggatg atcgtaacat aggaagaaag gctcatctta aaactaatat tggcgacaga
     1321 atgtaaaaag ggagatgtct cagaccgcag gtgtgagatc atccccgcgc cgcaggaacg
     1381 ggatataata cagttcatgc ccaacaacgg agaaggtgta ctccagctcc tgcagggttc
     1441 cggaaagatc gatctcatgt ttcaggaaca ggaactccgc atccttctcg gcatttttgg
     1501 aactcttcag tgc
//
LOCUS       DALTZZ010000234         1669 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_38531,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000234 DALTZZ010000000
VERSION     DALTZZ010000234.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38531"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            177..1667
                     /gene="cls"
                     /locus_tag="O0X12_04510"
     CDS             177..1667
                     /gene="cls"
                     /locus_tag="O0X12_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HJK53449.1"
                     /translation="MFDILLTLITEGVAWFLVLLVLLVDIGACITLLFLERRNPQVVA
                     GWLLLLIFLPIVGFVVYLFFGRHLYGEHIFSKKTAADESIAEIAGDQYQKILEKNMPL
                     PPEIERFDSTMALLLNQDNAAYSDNNAVDVYVHGEDKFAAFKEAIRSAQHHIHMEYYI
                     LRGDELGREIVDLLAERAAAGVEVRALFDAVGVQKVKREFFANLRAAGGEVAIFFPLK
                     IPFLNTRINFRNHRKILVVDGKTGFVGGFNIGDEYLGKGPLGYWRDTHLRLHGGSVAG
                     LQTRFIMDWNYAAPKSPVVVRDNSPYYPAETLSFPHGSSGVQIASSGPDSAGRAIYAG
                     FVSLIGHARKSIYIHSPYFIPDETIFTSLLMAIRSGIDVRIVIPCKPDHPFVYWASYS
                     YLGDLIRAGAKGYTYDNGFIHSKTAVIDGVACAIGTANWDIRSFKLNFETDAFVYDET
                     FGRKMDELMRKEMEEKCTLVTVEMYNNRPKWIKFKEGICRLVSPLL"
BASE COUNT          412 a          515 c          399 g          343 t
ORIGIN      
        1 gcatacccac ccactcaccg cctccgatca gaagaccggc aatgtcagca atgattgcgg
       61 caagtaaaaa tcccctgccc caaagattac agaactgttt gtaggataat gccatcgctg
      121 atccatacgt gtgcagggaa catatacttc tccgtgcata taatagaaca taacgcatgt
      181 ttgatatcct gctgaccctc atcaccgaag gagttgcctg gttcctcgtc ctcctcgtcc
      241 ttcttgtgga cattggtgcc tgtatcaccc tgctgtttct cgaacgaaga aacccgcagg
      301 tggttgccgg atggctgctt ctgctgatct ttttgccgat tgtaggattc gtagtgtacc
      361 tgttcttcgg ccgccacctc tacggagaac acatcttcag caaaaaaacc gccgccgacg
      421 aaagcattgc cgagattgcc ggggatcagt accaaaagat cctcgaaaaa aatatgccgc
      481 tgccgccgga gatcgaacgc ttcgacagca ccatggccct tcttcttaac caggacaacg
      541 ccgcctactc ggacaacaac gcagttgacg tctatgtcca tggtgaagac aagttcgccg
      601 cattcaaaga ggccatcaga tccgcacagc accacatcca catggaatac tacattctcc
      661 ggggcgacga actcggtcgt gaaatcgttg accttctggc tgaacgtgcc gcagcaggcg
      721 ttgaggtgcg ggcactcttc gatgcggtcg gcgtacagaa agtaaaacgc gagttcttcg
      781 ccaacctccg ggcagcaggc ggtgaggttg ccatcttctt cccgctcaaa atcccgttcc
      841 tgaacacccg catcaacttc cgcaaccacc ggaagattct cgttgttgac ggaaaaaccg
      901 gatttgtcgg cggattcaac atcggtgacg agtacctcgg caaaggaccg ctcggctact
      961 ggcgcgacac acacctgcgg ctccacggcg gatcggttgc aggcctgcaa acccgcttca
     1021 tcatggactg gaactacgcc gcacccaaat ctcccgtagt tgtcagggac aacagcccgt
     1081 actacccggc agaaaccctc agcttcccgc acggctcctc cggcgtacag atcgcatcct
     1141 ccggtcctga ctccgcaggc agagcaatct acgcaggatt cgtcagcctg atcggtcacg
     1201 ccagaaaatc catctacatc cactcaccgt acttcatccc ggacgaaacg atcttcacct
     1261 cactgctgat ggcaatccgt tccggcatcg acgtgcggat cgtcatcccc tgcaaaccgg
     1321 atcacccgtt cgtttactgg gcaagctact cctacctcgg tgacctgatc cgggccggag
     1381 caaaaggcta cacctacgac aacggattca ttcacagcaa aaccgcggtc atcgacggcg
     1441 tcgcctgtgc catcggtacc gcaaactggg acatccgcag cttcaagctc aactttgaaa
     1501 cggacgcctt tgtgtacgac gaaacattcg gcagaaaaat ggacgagctc atgagaaaag
     1561 agatggagga aaaatgcacg ctggtcaccg ttgagatgta caacaaccgg ccgaaatgga
     1621 tcaaattcaa agaaggcatc tgccgcctcg tctcgccgct gctgtaacc
//
LOCUS       DALTZZ010000235         3162 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_39022,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000235 DALTZZ010000000
VERSION     DALTZZ010000235.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39022"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="O0X12_04515"
     CDS             <1..830
                     /locus_tag="O0X12_04515"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HJK53450.1"
                     /translation="IPLRDRLRYILTKGDRTEDFYRYQYERFFARFYSPEERCLRIGP
                     LRLPRLSVSEHPTREDAYYAMEIGDILIPALFQRYGYVDEGPYEWGDVRIARDDVVFD
                     CGANLGVFTVLAAYRGAEVFAFEPIPEARTILRQTLKLNPALADRVHVVPCALGDEEK
                     TAEFTILADTLVGSSMVLPQTGRKMRVPVTTVDAFVESEGLDAVNFIKADVEGAERQM
                     LAGAVDTLRNYTPKVAICTYHLADDPLVIANLLRNADPRYQLTSRWKKMYGVVPSRR"
     gene            913..997
                     /locus_tag="O0X12_04520"
     tRNA            913..997
                     /locus_tag="O0X12_04520"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:949..951,aa:Leu,seq:cag)
     gene            1164..1994
                     /locus_tag="O0X12_04525"
     CDS             1164..1994
                     /locus_tag="O0X12_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HJK53451.1"
                     /translation="MKSIFSAPLHILFDDAACIRCGLCATMCTTGKIVASETKSPKIC
                     DDTPCLSCGQCIAVCPQAALSTDTPAFVTPADNSAYKTGINPDLLSTYLKSRRSVRIW
                     KEKEVAQKYLEQLIGIAAYAPSACNIHPVKWVVISDPATIRKFTDASITALKALPPDH
                     PLAQLSQMLIANADNGSDPICRSAPALLIAASDADQEYGLIDSIIALSYIDAFAPSIG
                     LGTCWVGYVMIMLRIKPELGEILGIPKNWVPQYAMLAGYPGVSFSQIPPREIPEVIWK
                     "
     gene            complement(2167..2787)
                     /locus_tag="O0X12_04530"
     CDS             complement(2167..2787)
                     /locus_tag="O0X12_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HJK53452.1"
                     /translation="MARKKQSGGRKVEGWKAKSWFKVYAPEFLGKQFIGEIVSSDTAN
                     LPGRVLSVSLGELIQDYSKQNVKISFKINDVAGDAAYTQFHGHEMTKDFIRAMVKRRA
                     SRIDSTITIQPLGSVRELQVTITAFTINHARLSQIHEIRAMMVKVVQDYAKEADFETF
                     VSAMVKGELSKKMLEACKPIFPIRKIEVIKSESVSSAAERAAALVR"
     gene            complement(2839..3045)
                     /locus_tag="O0X12_04535"
     CDS             complement(2839..3045)
                     /locus_tag="O0X12_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HJK53453.1"
                     /translation="MYISGTITSAHVHPEHVVSALSPDSDGFMTVEVLDGCIVARVEG
                     ESLRSVIATVDDYLMNLSVSEQLC"
     gene            complement(3036..3161)
                     /locus_tag="O0X12_04540"
     CDS             complement(3036..3161)
                     /locus_tag="O0X12_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HJK53454.1"
                     /translation="MKATAEACGGSGGGHQFRAGADIPLDREDEFLACLEAAACT"
BASE COUNT          831 a          805 c          733 g          793 t
ORIGIN      
        1 atattcccct gcgcgatagg ctccgataca ttctgacaaa aggagaccgc accgaggact
       61 tttaccgtta ccagtatgaa cggttttttg cacgattcta ctccccggag gagagatgtc
      121 tgcgtatcgg gccactccgt ctgccgcgtc tgtccgtatc ggaacaccca acacgcgagg
      181 atgcatatta cgcaatggag ataggagata ttctcatccc agctttgttt cagaggtatg
      241 ggtatgtgga cgaggggccg tatgagtggg gagatgtacg gattgcgcgt gatgatgtgg
      301 tgtttgattg cggtgcaaac ctcggtgtgt ttactgtact tgccgcatac cgtggagccg
      361 aagtgtttgc atttgaaccc ataccagagg caagaacgat cctaagacag acactaaagc
      421 tgaaccccgc ccttgctgac cgggtacatg tggtcccctg cgcactgggt gatgaggaaa
      481 aaactgcgga gtttactatt cttgcagata cactggtcgg atcgtcgatg gtactaccac
      541 aaacaggcag aaagatgcgt gttccggtaa caacagtaga tgcatttgta gaatcagaag
      601 ggctggatgc ggtgaacttt atcaaagcag atgtagaagg ggcggagcgg cagatgcttg
      661 ctggagcggt ggatactctg cgaaactaca ccccaaaggt agctatctgt acctatcatc
      721 ttgcggatga tccgcttgtg attgctaatc tgctccggaa tgcggatccc cggtaccaac
      781 tgacctcacg atggaaaaag atgtacgggg tggttccatc tagaagataa tatgcctaaa
      841 tttaagtatg agaatcacaa atatagtagg gcacgaagat aatcctgttt gtaggatgtt
      901 ttctattttc atgcgaaggt agccaagcca ggtcaacggc gccagattca gggtctggtc
      961 tcataggagt tctggggttc gaatcccttc cttcgcactt gtttgtcgta ccattttgaa
     1021 aaagtacaat acgcgatgat tttaccagtt tttttccaaa ccatgaacct tatgtaagta
     1081 tatcacaact aacagataga ccgaaagtcc agcgggtgtc agaacccaaa actctatcac
     1141 ccgcacgttc cagagtattt ctgatgaagt caatattttc cgcaccattg catatcctct
     1201 ttgatgatgc ggcctgtatc agatgcgggt tgtgcgcaac aatgtgtacg accggcaaga
     1261 tcgtcgcgtc agaaacaaag tccccaaaaa tctgtgatga cacaccctgt ctctcctgcg
     1321 gacaatgtat tgcagtttgc cctcaagcag cattatccac ggatactcct gcatttgtca
     1381 caccagcaga taattctgca tacaaaacag gaataaaccc tgatcttctg tcaacgtacc
     1441 tgaaaagccg cagatcagta cgtatctgga aagaaaaaga ggttgcgcag aagtatcttg
     1501 aacaactcat cggaattgca gcgtatgcac cgtcagcctg caacatccat ccggtaaagt
     1561 gggtggtgat ctctgatcct gcaacgatac ggaagtttac agatgcctca atcaccgcgc
     1621 tgaaagcact cccccctgat catcctctgg cacaactctc acagatgctg attgccaatg
     1681 cagacaacgg atccgatccc atctgccgca gcgccccggc tctcctgatc gccgcgtccg
     1741 atgcggatca agagtatgga ttaatcgaca gtatcattgc gctgtcctac attgatgcat
     1801 ttgcaccgtc aatcggcctt ggtacctgct gggtcggtta tgtcatgatc atgctgcgca
     1861 taaagcctga gttaggagag atacttggaa tccccaaaaa ctgggtaccg cagtatgcga
     1921 tgcttgcagg ataccccggt gtctcctttt cacagattcc tccgcgagaa atcccggagg
     1981 ttatctggaa ataatgcatt tacattttcc aacatgcaac aaatactact gcaaaaaaaa
     2041 caggacacat cggttcactc atcatttatc ctagaaatta tatttcagac tgagatcgcc
     2101 catctatcgt gaaaaaatgg gtctcggttg tcacaagaga attaaaaaag aaaagtatcc
     2161 gttgatttaa cggacaagtg ctgctgcacg ttctgctgca gaacttacag actcggactt
     2221 gatgacctcg atcttacgga tcgggaagat cggcttgcat gcctcgagca tcttcttgga
     2281 aagctcaccc ttaaccattg cggagacaaa ggtctcaaag tctgcttcct ttgcatagtc
     2341 ctgcacaacc ttcaccatca ttgcacgaat ctcgtgaatc tgggaaagtc tggcgtggtt
     2401 gatagtgaag gcggtaatcg tcacctgaag ttcacgaacg ctgccaagcg gctggatcgt
     2461 aatggtgctg tcgatacggg atgcccgcct cttcaccatt gcacggatga aatctttggt
     2521 catctcgtgg ccgtggaact gcgtgtatgc tgcatctcct gcaacatcat tgatcttgaa
     2581 cgaaatcttc acattctgct tggagtagtc ctgaattaac tcaccaagac taaccgacag
     2641 tacacgaccc ggcaggtttg ccgtgtcaga ggagacgatc tccccaataa actgcttgcc
     2701 gaggaactcc ggggcgtaaa ccttgaacca gctctttgcc ttccatccct caactttgcg
     2761 tccaccactc tgcttctttc ttgccatggt atcaccttaa tttttgaaat tgtatttttg
     2821 tacccggaaa ccgtgcatct aacacaactg ttccgagacg gataaattca tcagataatc
     2881 atccaccgtc gcaatcaccg atcgaagcga ctctccctct actcttgcca caatacaacc
     2941 atccagaacc tcaacagtca taaacccgtc cgagtcagga gacaatgccg agactacatg
     3001 ctctggatga acatgggctg acgtgattgt cccgcttatg tacatgctgc tgcctccaga
     3061 caggcaagaa actcatcttc acgatcaagc ggaatatctg ctccggcacg gaactgatgc
     3121 ccaccgccgg aaccaccgca agcttctgct gttgctttca tg
//
LOCUS       DALTZZ010000236         2412 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_39034,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000236 DALTZZ010000000
VERSION     DALTZZ010000236.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39034"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="O0X12_04545"
     CDS             complement(<1..851)
                     /locus_tag="O0X12_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJK53455.1"
                     /translation="MYDVIIAGAGPTGSAAARFCAKAGLSTLVLEEHAAIGYPVQCAG
                     LLSNSAFAECGVSDRSVLNTVRGARICGSAGHELSFTAKETKAYVVDRGRLDHEMAVR
                     AADAGAIFSLKTCVTGINPEKRTIRTTGVAGKQELSYNILIAADGPRSVIARGLGIPI
                     SRYIYAGIQAEVSWNGSSDLVELHPNASPDFFAWVIPLSSTRARIGLCGMRDVPERFA
                     AFVKQFSSSNIHEVTGTIPIGVRRRTYGAGCMLAGDAAGFPKPTSGGGVYTGVRSARH
                     AASVAVAA"
     gene            complement(900..972)
                     /locus_tag="O0X12_04550"
     tRNA            complement(900..972)
                     /locus_tag="O0X12_04550"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(936..938),aa:Glu,seq:ctc)
     gene            1085..2197
                     /locus_tag="O0X12_04555"
     CDS             1085..2197
                     /locus_tag="O0X12_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HJK53456.1"
                     /translation="MSLEEEIKAIEDEIRNTKYNKATSQHIGRLKAKLAKIKDDAVTR
                     AMKSAGSGEGYAVKKSGDGTIVLVGFPSVGKSTLLNKLTGAESETGAYAFTTLTVVPG
                     LMEYKGAKIQILDIPGLIAGAAMGKGRGKEVIAVVRTADLIVILGDVFNGVHVDVLMH
                     ELYDAGIRINKPKPDITIKKTGYGGVRLNTVGAVNIDIEEVRAILAESKVMNADVLVR
                     GSNVTQDDIIDAMMGNRMYIPAFIAINKVDLVTEKERQEVENEMREKYGVDPIMISAH
                     AGYNIDKLQEAIYEHLGFVRIYMKPYGEEADMEEPMIMRKGSTIEDICRRLHRDFVDQ
                     FRYAKIWGTSVKHDAAKVGLQHKVEDGDIVTIVTKL"
     gene            complement(2208..>2412)
                     /locus_tag="O0X12_04560"
     CDS             complement(2208..>2412)
                     /locus_tag="O0X12_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53457.1"
                     /translation="LIIRRVLAVRKVRPGMVDNIIFAGSVAALVAANIPALVDGVKQV
                     AAELRSLQQIIEKTDGGIEIPIA"
BASE COUNT          674 a          572 c          652 g          514 t
ORIGIN      
        1 gcagcaaccg caacagatgc cgcatggcgg gcggagcgta cgccagtgta tactccgccg
       61 cctgatgtgg gttttggaaa gccggcggca tcccctgcta acatgcagcc cgcaccatag
      121 gtacggcggc gtacaccaat cggaatggtg ccagttacct catggatgtt tgaggaagag
      181 aactgtttta caaatgccgc aaaacgttcc ggtacatccc gcatgccaca aaggccaatc
      241 cgtgcgcggg tggaggaaag cgggatcacc caggcaaaaa agtctggtga tgcgttcggg
      301 tgtaactcca cgagatcaga actcccattc caggaaacct ccgcctgtat ccctgcataa
      361 atataacgtg atatagggat tccaagtccg cgggcaataa cactacgcgg cccgtccgct
      421 gcaatcagaa tattgtagga aagttcctgt tttccggcaa caccggttgt gcggatcgtt
      481 cttttctcag gattgattcc tgtcacacag gttttcagcg aaaagatagc acctgcatct
      541 gctgcacgga ctgccatttc atgatccagc cgtccacgat ccaccacata cgctttggtt
      601 tcttttgcag taaaggaaag ttcgtgcccg gcggaaccac agatacgggc accgcgaacg
      661 gtgttcaaaa ccgatctgtc agacaccccg cactccgcaa atgccgagtt ggagagaaga
      721 cccgcacact ggactggata accgattgca gcatgttctt ccagtaccag agtggagaga
      781 ccagctttgg cacagaaccg tgcggcggct gaaccggtcg gaccggcacc ggcaatgatg
      841 acatcgtaca tggaaaagat acagagatgt tatcagtcag agaagataaa caaaaggagt
      901 gctgatatgg ggattcgaac cccagtcgtc ggcgtgagag gccgacatga ttggccacta
      961 cactatatca gcaagagaac ttgcgtaact aatgttgccg tatgaaatat ttatagatac
     1021 cgattgaaaa gcacacactc ccccatatgt tcatctaata ccaaaacgta taggtataga
     1081 caatatgagc cttgaagaag agatcaaagc cattgaggac gagatcagaa ataccaaata
     1141 caacaaggca acttcacagc acatcgggcg gttgaaagca aaactcgcca agatcaagga
     1201 cgatgccgtc acgcgcgcaa tgaagtctgc cggcagcggt gagggatacg cagtcaaaaa
     1261 atccggtgac gggacaattg tgctggttgg ttttccttca gtcggtaaat caacactgct
     1321 caataaactg accggtgcag aaagtgagac cggtgcatat gcgttcacca cactgacggt
     1381 tgttccagga ctgatggagt acaaaggtgc aaaaattcag atcttggata ttccaggtct
     1441 gattgcgggg gcagcgatgg gcaagggacg cggtaaagag gtaattgccg ttgtccggac
     1501 agcggatctg atcgtgatct tgggtgatgt gttcaacggt gtgcacgttg acgtgctgat
     1561 gcatgagctg tacgatgcag gaatccgcat caacaagcca aaaccggata tcaccattaa
     1621 aaagaccgga tatggcggtg tccgcctgaa tacagtcggt gcggtgaata ttgatattga
     1681 ggaagtccgt gcaattctcg ctgagagtaa ggtgatgaac gcggacgtac tggtccgcgg
     1741 cagtaatgta acccaggatg atatcattga tgcaatgatg ggcaaccgga tgtacattcc
     1801 ggcattcatt gcgatcaaca aggttgatct tgtaacggaa aaagagcggc aagaggtgga
     1861 aaatgagatg cgggagaagt atggtgtcga tcccatcatg atctcggcac acgcagggta
     1921 taacatcgac aaactccagg aggccatcta cgaacatctc gggtttgtgc gtatctatat
     1981 gaagccgtac ggagaggagg cggacatgga agagccgatg atcatgcgga aaggcagtac
     2041 gattgaggat atctgccgca gacttcaccg tgattttgtg gatcagttcc ggtatgcaaa
     2101 aatttggggc acatcagtaa aacacgatgc cgcaaaggtc ggccttcagc acaaggtgga
     2161 ggacggcgat atcgtgacaa ttgtcacaaa actctaaaaa atatttttta ggcgatcgga
     2221 atttcgatac cgccatcagt tttttcgatg atctgctgca ggctccgcag ttcggctgcg
     2281 acctgcttta caccgtccac taatgccggg atgtttgcgg caacaagcgc ggcaaccgat
     2341 cctgcaaaga tgatgttgtc aaccatacct ggacgaactt ttcgtacagc aagaacgcga
     2401 cggatgatca gc
//
LOCUS       DALTZZ010000237         1776 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_39056,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000237 DALTZZ010000000
VERSION     DALTZZ010000237.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39056"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            53..1063
                     /locus_tag="O0X12_04565"
     CDS             53..1063
                     /locus_tag="O0X12_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HJK53458.1"
                     /translation="MEIRKVQVTGGSSYVISLPKGWVRQQKIEKNDPVGVIAQADGTL
                     LLTPNLTYDSTTRIREFSLKDYPVPDMLLRSLIGAYISGFTTIRITSAGRIPPKVRQV
                     VRKFTQMTIGQEVAEESDNSIILKDILNPSEMPFENTIRRMYVIVKAMHEDALYALEQ
                     GRRELAEDVSARDTDVDRLHWLVHRQFSLIVTNPALSRRMEIAPAKAATYYQVSRIIE
                     RVGDHAVAISEAALVLIDMRVDHTVVAKITKAGEASLDIFNRSIKAIYANSIPEANAI
                     IEAADDLLPEYHTLSAMTQKMKVKEAVAMTTIANSMHRISEYSSDIAEILINQMVGTD
                     GT"
     gene            1195..>1776
                     /locus_tag="O0X12_04570"
     CDS             1195..>1776
                     /locus_tag="O0X12_04570"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HJK53459.1"
                     /translation="MFIMATGIALSIRADMGITPISSLPYVVSLGVPLTVGMLTILLH
                     AGFLLIEFLLMPREFEPIQLLQLGAAVVFGAFIDLVLAGLAWLVPVSYPEQILLVVLS
                     CALLGVGICFEVAPNVLMMASEGMLTAAVRRFHVEFGTIKVALDVTLVVLSIILSLAM
                     FSTVYGIREGTVIAAVLVGIFVKLFKLPVYAATQ"
BASE COUNT          457 a          399 c          469 g          451 t
ORIGIN      
        1 gaatctataa gtatcaataa atatttacta tgacatagag tattgcagat ctatggaaat
       61 tcggaaagtg caggttaccg ggggttcgtc atatgtgatc tccctgccaa aaggatgggt
      121 acgccagcag aaaatcgaga agaatgatcc tgtcggtgta attgcccagg cggacgggac
      181 actgcttctc acaccaaatc tgacttatga cagtacgaca cggatccggg agttttccct
      241 aaaagactat ccggttccag acatgctgct cagatccctg atcggtgcat atatttccgg
      301 atttaccacc attcgcataa catctgctgg aagaatcccg ccaaaagtgc ggcaggtagt
      361 acggaagttt acacagatga ccattggcca ggaggttgcc gaggagagtg acaacagcat
      421 catcctaaaa gatatcctga acccttccga gatgccgttt gagaatacga tccgccggat
      481 gtatgtaatt gtaaaggcga tgcacgagga cgctctgtat gcactggaac agggacgtcg
      541 tgagctcgcc gaggatgtct ctgcccgtga cacggatgtg gatcgcctgc actggctggt
      601 acatcggcaa ttttcgctga ttgtcacaaa tccagccctc tcgcgaagaa tggagattgc
      661 ccccgcaaag gctgcaacct actatcaggt ttcacgaatc attgaacgag tgggagatca
      721 cgctgtggca atctctgagg ctgcactggt gttaattgat atgcgggttg atcacacagt
      781 tgtcgcaaag ataacaaaag caggtgaagc atccttggac atcttcaaca ggagcatcaa
      841 ggcgatttat gcaaacagta tccctgaggc aaacgcaatc attgaggcag ctgatgatct
      901 gctgccggag tatcacacac tctctgcgat gacccagaag atgaaggtaa aagaggcggt
      961 tgcaatgacc acaattgcaa acagtatgca ccggatcagt gagtacagct cggacattgc
     1021 agagatcctg atcaatcaga tggtcggaac cgacggaacc tgagtacaca gcaaaagagt
     1081 agtactcccg cagcaagaaa aattcacaaa tactttcgcc gcaaatatag atccacgcaa
     1141 ccgaaatcag tatatttcaa attagcccga aaatatggtg ttttcatctt cgggctgttc
     1201 attatggcta ccggtatcgc cttatcgatt cgtgcagata tggggattac gcctatatcc
     1261 agtttgccgt atgttgtgag tcttggtgtt ccactgacgg ttgggatgct gacaattctg
     1321 ctgcatgccg ggtttctgct gattgagttt ctgttgatgc cgcgggagtt tgaaccgatc
     1381 cagttgcttc agctgggagc ggcagtggtg tttggggcgt ttattgatct ggttcttgca
     1441 ggtcttgcct ggctggtacc ggtcagttat ccggaacaga tactgcttgt tgtgctgagc
     1501 tgtgccctgc ttggtgtcgg tatctgtttt gaggtggcac caaacgtact gatgatggca
     1561 agcgaaggta tgcttaccgc agcagtccgc aggttccatg tggagttcgg gacaataaag
     1621 gtcgctctgg atgtaacgtt agtggtgctc tcaattattc tgtcacttgc aatgttttcc
     1681 acagtgtatg gcatccggga aggaacagtg atagccgcag ttctggtggg tatctttgtg
     1741 aaactgttca aactgccggt ctatgcggca acacaa
//
LOCUS       DALTZZ010000238         4913 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_39151,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000238 DALTZZ010000000
VERSION     DALTZZ010000238.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4913)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4913)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4913
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39151"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            130..522
                     /locus_tag="O0X12_04575"
     CDS             130..522
                     /locus_tag="O0X12_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HJK53460.1"
                     /translation="MILKGRSIAKGTGSGELLYTDTPISFLGGVDAATGIIIDEKHPL
                     HGQSVAGKVLAFPYGKGSTVGSYVMYGLARNNVAPAAIINTECETIIAIGAIISGIPM
                     VDRLDGDVSALSGTITVNGSTGEVSTAE"
     gene            519..1616
                     /locus_tag="O0X12_04580"
     CDS             519..1616
                     /locus_tag="O0X12_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HJK53461.1"
                     /translation="MKAVWRYIHAARNSYAALYAACEAYGFILEPVDAPEGDVVCYSL
                     NSIEFPRYRDEIAAAEQITIAGGPHASACWEEVAEVADYVIIGEGERTLPRLLSALGE
                     GESGALLPGVATREGGRNKIDHTVRLDGFPCFTRMKGYMEISRGCPFHCGYCQTPRLH
                     GTKMRHRSREAIVEAASHYRDARFVTPNAFAYGSADGKTPDVEKLERLLASMPDNNLY
                     FGTFPCEVRPEFVTQETVDLVVQYCANTKLHFGAQSGSDTVLAKMGRGHSLDDVYTAL
                     DVCRGAGLEPVVDVIFGFPFETDEDEEATLALVKEVVRCGKVHVHYLMPLPGTPLSRA
                     VPREVIPAVDRELGKLALSGRVTGAWHNKFD"
     gene            1672..2034
                     /locus_tag="O0X12_04585"
     CDS             1672..2034
                     /locus_tag="O0X12_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53462.1"
                     /translation="MTLEPKDALTQYHFAERLKLDIMMIAHQYLTIPSLKKEEKAGAK
                     RVLINITEALAADSHAAAAFTGCDDFNTAAGVLDEVMNLAESNQFGLASEKAGEAMVP
                     ITTAAAAAWEVLHNHGLL"
     gene            2021..2548
                     /locus_tag="O0X12_04590"
     CDS             2021..2548
                     /locus_tag="O0X12_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HJK53463.1"
                     /translation="MDSSEFLNFLATRVSVREYEEGCITEDDASYLLTAASKAPSAGN
                     LEAWDVVIVTDPGQLEMLADAAGNQHQIKDAGSVFCVCANYVRAMSRYGERGIMFAVE
                     DATIAATYMMLAAHARRLHTCWVGAFDDGEVKGILDLPAHIRPVALLCVGRGEAPSRG
                     PERMDPEGHAHYDIW"
     gene            2573..2932
                     /locus_tag="O0X12_04595"
     CDS             2573..2932
                     /locus_tag="O0X12_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF555 domain-containing protein"
                     /protein_id="HJK53464.1"
                     /translation="MPDYRVTLEAAWTVKDVARTQDAIGIAVSEAGKRLHPSAKFVDV
                     DVMVMPCPYCGEGINSALVVARTGIVGLLLSMKVFNAEGEEHAERIAKSVIGRAVRDI
                     PLATYSITTCEAEEETA"
     gene            2929..3828
                     /locus_tag="O0X12_04600"
     CDS             2929..3828
                     /locus_tag="O0X12_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HJK53465.1"
                     /translation="MNDPIISVCGHLCNDYIIAVEEYPAVGTSCRVTYRQNYYGGGAA
                     NIAVGIAKLGGTAELIAAVGRDYPGSAYEQHLKEMGVTTRLFRTDKENCSTAFMVNNA
                     AGDQITYFEWGASALFATADAPSLSFAHMATGDADFNVKVARQAEFATFDPGQDVKYY
                     EASHLREIFENIDMLICNNFEAEIMCGKLGWTEKELIASVPVAILTKGKDGSVLYQND
                     DVVEIPACPVTMVDPTGAGDAYRAGLLTAYRRGYALPVCCKIGAVTSSFAVEKVGTQT
                     NLANWEQMRERYAEQFGTLKKSE"
     gene            3825..4481
                     /locus_tag="O0X12_04605"
     CDS             3825..4481
                     /locus_tag="O0X12_04605"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR03679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HJK53466.1"
                     /translation="MTWAALTSGGKDSILALQKALDAGMDVSYMVTVVPENPDSYMFH
                     SANLAAVPVIAERCGMTYVGIPTLGEKEDELLDLEQGLAALPIEGVIVGAIESEYQRS
                     RVAALCDRLGLVMFAPLWHMDPLKLLYEVAERMDVRIVVTAADGLGENVLGKRIDADL
                     IDLLCRIAAKRRIHIAGEGGEYESLTLDAPCFSSLIETEGWTMTFSCGRGVAAIEKFW
                     "
     gene            4490..>4913
                     /locus_tag="O0X12_04610"
     CDS             4490..>4913
                     /locus_tag="O0X12_04610"
                     /inference="COORDINATES: protein motif:HMM:NF021370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HJK53467.1"
                     /translation="MATEAKTENLSKYLFSAPVWWQSLIIIIVLGLLVDGAVYVLSGG
                     TGTMMYGVAFAAAAVAAMLLTKPLVDLLGTEKLTWNRSALVALASMIFSLLWMAIGFA
                     LNAALAYVLGLGFILAIRLMVLTAICDYRIGRMFLPAAV"
BASE COUNT         1159 a         1245 c         1397 g         1112 t
ORIGIN      
        1 ggtcatagca gtccgtttgt tgcagagttt gtgaacgaaa atttcgttac cgaatttgtc
       61 ggttgtatag atataagagt gtaatggttt tgtgaggctt taatgccgga gaggcacata
      121 tatagaggta tgattctgaa aggccgcagc attgccaaag gtacgggatc cggcgagctt
      181 ctctataccg atacacccat ctccttcctt ggcggcgttg atgccgcaac cggcattatc
      241 atcgatgaaa aacatccgct gcacggacaa tccgttgcag gaaaggttct cgcattcccc
      301 tacggaaagg ggtccaccgt tggctcatac gttatgtacg gccttgcaag aaacaacgtg
      361 gcgccggctg caatcatcaa cacagagtgc gagaccatca tcgcaattgg tgcgatcatc
      421 tccggcattc cgatggttga ccggctggat ggtgatgtat cggccctctc cggaacgatc
      481 accgtcaacg gcagtaccgg tgaggtttcc accgcagaat gaaagcggtc tggcgctaca
      541 tccatgcggc acgaaacagt tatgccgcat tgtatgcggc gtgtgaggca tacggattca
      601 ttctcgaacc ggtggatgca ccggagggtg atgtcgtctg ctacagcctc aacagcattg
      661 agtttccgcg ctaccgtgac gagatagcag cagcagaaca gatcacaata gccggaggac
      721 cgcacgcctc cgcctgctgg gaagaggtcg ccgaggtcgc ggattatgtg ataataggtg
      781 aaggggaaag aacccttccc cggcttttgt ccgctctcgg ggaaggagag tcaggagctt
      841 tgctgcccgg cgttgcaacc agagaaggcg gcagaaacaa aattgatcac accgtccggc
      901 tggatggatt cccctgtttt acccggatga agggctatat ggagatctcc cgcggctgtc
      961 cgtttcactg cgggtactgc cagactccgc gtctccatgg aacaaagatg cgccaccgct
     1021 cacgtgaagc gatcgtggag gctgcaagtc actaccgcga cgcacgtttt gttaccccga
     1081 atgcctttgc ctatggatca gctgacggaa aaactcccga cgtggaaaaa ctggaacggc
     1141 tccttgcctc catgcctgac aacaacctct acttcgggac cttcccttgc gaagtacggc
     1201 ctgagtttgt cacacaggaa acagtggatc ttgtggtgca gtactgtgcc aacaccaaac
     1261 ttcacttcgg tgcacagtca ggatcagata ccgttcttgc aaaaatgggg cgcggccact
     1321 cccttgacga tgtgtacacc gcactggatg tctgccgggg tgcagggctt gagccggtgg
     1381 ttgatgtcat ctttggtttt ccgtttgaga cggatgagga cgaagaggca acgcttgcac
     1441 ttgtgaaaga ggtagtcaga tgcggaaaag tacatgtcca ttacctgatg ccgctgccgg
     1501 gaacaccact ctcccgtgca gttccccgcg aggttatccc ggcggtggat cgggaacttg
     1561 gaaaacttgc cctcagcgga cgtgttaccg gtgcgtggca caacaaattt gactgaaccg
     1621 gtttgcccga atactacatt tttattgatt ctccgcgcca actcgtaagg catgacactt
     1681 gaaccaaagg atgccttaac ccaataccat tttgccgaga ggttaaagct tgacatcatg
     1741 atgattgcac atcagtacct caccatcccc tcactcaaaa aagaggaaaa agcaggtgca
     1801 aagcgtgtcc tcatcaatat caccgaagcc ctcgccgcag acagccatgc agccgcggca
     1861 tttaccggtt gcgatgattt caatacagcc gcaggtgtcc ttgatgaagt aatgaacctc
     1921 gccgagtcca accagttcgg cctcgcttcg gaaaaagcag gagaagccat ggttccgatt
     1981 accaccgcag ccgcagccgc atgggaagtg ctccacaacc atggactcct ctgaatttct
     2041 gaactttctt gcaacccgtg tttcggttcg cgagtatgag gagggctgta tcaccgagga
     2101 cgatgcctca tatcttctga ccgcagcatc caaagcgcca tcagccggaa atctggaagc
     2161 gtgggacgtt gtgatcgtca cagatcccgg acaactggag atgctggctg atgctgccgg
     2221 aaaccagcac cagatcaaag atgcaggcag tgtgttctgc gtctgtgcaa actatgtccg
     2281 cgctatgtcc aggtacggcg agcgcgggat catgttcgcg gttgaggatg caaccattgc
     2341 ggcaacctat atgatgcttg ccgcccatgc acgccgactg cacacctgct gggttggtgc
     2401 gtttgatgac ggcgaggtaa aaggtattct ggatctcccc gcacacatcc gaccggtagc
     2461 actgctctgt gtcggccgcg gggaggctcc gtcacgcgga cccgaacgca tggacccgga
     2521 aggccatgct cactatgata tctggtagac accaataata caaataagat atatgccgga
     2581 ttatcgtgtg acactggaag ccgcatggac ggtaaaggac gtggcaagaa cacaggacgc
     2641 catcggtatt gccgtaagcg aggcaggcaa acgcctgcac ccctctgcca agttcgtgga
     2701 tgttgacgta atggttatgc cctgccccta ttgtggagag ggaatcaaca gcgcactggt
     2761 tgtggccagg accggtattg tcggactgct tctgtccatg aaagtgttca atgccgaggg
     2821 agaggaacac gcagagcgta tcgcaaaatc cgtaatcggc cgggcggtcc gcgacatccc
     2881 gctcgcaacc tacagcatca ccacctgcga agccgaggag gagacggcat gaatgatcca
     2941 ataatttccg tttgcggtca cctctgcaat gactacatca ttgcagtaga agagtatccg
     3001 gctgtaggaa cgtcatgcag ggtaacgtac cggcagaact actacggcgg aggagcggca
     3061 aacatcgctg tgggtatcgc aaaactcgga ggaaccgccg agctgattgc agcggttgga
     3121 agggattacc caggaagcgc ctatgagcag catctcaaag agatgggcgt tacaacccgc
     3181 ctctttcgta ccgacaaaga aaactgttca accgcgttca tggtgaacaa tgccgcaggg
     3241 gatcagataa cctattttga gtggggtgca agtgcacttt ttgcaacagc tgatgctcca
     3301 tctctttcct ttgcacacat ggcaactggt gacgcagact tcaacgtcaa agttgcccgg
     3361 caggcggagt ttgccacctt cgatcccggc caggatgtaa aatactatga agcatcccat
     3421 ctgcgagaga tctttgaaaa cattgacatg ctgatctgca acaactttga ggccgagatt
     3481 atgtgcggca aactcggctg gacggagaag gaattgattg catccgtccc ggttgcaatt
     3541 ctcacaaagg gaaaggacgg aagtgttttg tatcaaaacg atgatgttgt ggaaatcccg
     3601 gcatgtccgg tgacgatggt ggatccgaca ggtgcggggg acgcataccg tgcaggtctc
     3661 cttaccgcat accggcgcgg atacgcactg cctgtctgtt gtaagatcgg ggcagtgacg
     3721 tcgtcctttg cggtggaaaa agttggtacc cagacaaatc ttgccaactg ggaacagatg
     3781 agagaacggt atgccgagca gttcggcacc ctgaagaaga gtgaatgacc tgggcagcac
     3841 tgacatccgg gggtaaggat tccattctcg ccctgcaaaa agcacttgat gcagggatgg
     3901 atgtctccta catggtgacg gtggttccgg agaaccccga ctcctacatg tttcactcgg
     3961 caaacttggc cgctgttccg gtgattgcag agcggtgcgg gatgacctat gtagggattc
     4021 ccacgttggg agagaaggag gacgaactcc tggatctgga acagggtctt gccgcactgc
     4081 cgatcgaggg tgtaattgtg ggagcgattg agtccgagta tcagcgcagc cgggttgctg
     4141 cactgtgtga caggttggga cttgtcatgt ttgcgccgtt gtggcacatg gatccgctaa
     4201 agcttctgta tgaagttgca gaacggatgg atgtgcgaat agtggtgacg gctgctgacg
     4261 ggcttggcga gaatgtactt ggaaaacgga ttgatgcgga tctaatagat ctgttgtgcc
     4321 gcattgcagc aaaacggcgg atccatatcg ccggtgaggg cggggagtac gagtcgctaa
     4381 ccctggacgc cccgtgtttt tcctctctga ttgagaccga ggggtggacg atgacgtttt
     4441 cctgcggcag aggagttgct gcaattgaaa agttctggtg ataggtgtca tggctactga
     4501 agcaaaaaca gagaatcttt caaagtactt attttcggca ccggtctggt ggcagtcact
     4561 gattattata atcgtgcttg gcctccttgt ggacggtgct gtctacgttc tgtccggcgg
     4621 aaccggaacg atgatgtacg gagttgcatt tgctgcggca gcggttgctg caatgcttct
     4681 gacaaaaccg cttgtggacc tgctcggtac agaaaaactt acctggaacc ggtctgcact
     4741 ggtggcactt gcatccatga tcttttccct gctctggatg gcaattgggt ttgcactgaa
     4801 tgcagcactt gcctatgtac tcggcctcgg atttattctt gccatccggc tgatggtact
     4861 tactgccatc tgtgattaca gaattggacg gatgtttctt cccgcagcgg tac
//
LOCUS       DALTZZ010000239         1753 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_39184,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000239 DALTZZ010000000
VERSION     DALTZZ010000239.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39184"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..979
                     /gene="ftsZ"
                     /locus_tag="O0X12_04615"
     CDS             <1..979
                     /gene="ftsZ"
                     /locus_tag="O0X12_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HJK53468.1"
                     /translation="SNTVARIFEEGVEGAEIYALNTDAQHLSMLRGRVGKRILIGRQT
                     TKGLGAGAMPQRGEEAALESEDIIRQSLNGSNMVFVTAGLGGGTGTGSAPIVAKIAKE
                     VGALTIAIVTLPFTSESATRMENAEIGLERLREVADTVIVIPNDRILEVVPRLPLSQA
                     FKVADEVLMRAVKGITELITLPGLVNLDFADVRTVMEQGGIAMIGMGESDSEDKATDS
                     IKKALRSPLLDVDITGATAALINVIGGPDMTMFEAEGVVQEVYQRIDPGARIIWGVQV
                     DPEMEGRMRTMLIVTGVQSPQIYGKQEQTYTASQSSQVQKSKFEIDFLR"
     gene            1042..1227
                     /locus_tag="O0X12_04620"
     CDS             1042..1227
                     /locus_tag="O0X12_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HJK53469.1"
                     /translation="MPEVSKQSISEFFRKYLRVLKLARRPTKDEFFKISAVAAAGIAV
                     IGVLGFIIYLVFHYLLP"
     gene            1232..1708
                     /locus_tag="O0X12_04625"
     CDS             1232..1708
                     /locus_tag="O0X12_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HJK53470.1"
                     /translation="MTDSEFGNHYYIIKTTSKHERTVADNIREAIENNMTDVVVTAVV
                     VPEDIKGYVFVESPEEHARIEELVETIAHARVVLKSETSMEEIKHFLVPKPAVSGIEE
                     GTIVELIAGPFKGEKAVVKRVDHTKEEITVELYESIVPIPITVRGDNVRVIDRSHE"
BASE COUNT          475 a          426 c          463 g          389 t
ORIGIN      
        1 atccaacacc gttgcacgca tcttcgagga aggtgtcgaa ggcgcagaaa tttacgcact
       61 gaacactgac gcccagcacc tctcaatgct ccgcggccgg gttggaaaac gcatcctgat
      121 cggacgccag acaacaaaag gactcggtgc cggtgcaatg ccgcagcgtg gtgaagaggc
      181 agcactggaa agcgaggata tcatccgcca gtccctcaac ggttcgaaca tggtgttcgt
      241 caccgcaggt ctcggcggcg gaaccggtac cggcagtgca ccaattgttg cgaagatcgc
      301 caaagaagtg ggggcactaa caattgcaat cgtcacactt ccgtttacaa gtgaaagcgc
      361 aacccgcatg gagaatgcgg agatcggtct tgagcggtta cgtgaggttg ctgacaccgt
      421 catcgtcatt ccaaatgacc gcatccttga ggtagtcccg cgtctgcctc tctcacaggc
      481 attcaaagtt gccgatgaag tcctcatgcg tgccgtaaaa ggaatcaccg aactgattac
      541 cctgccgggt ctggtaaatc ttgactttgc cgatgtccgt actgtcatgg aacagggcgg
      601 aattgctatg atcggtatgg gtgaatccga cagcgaggac aaagcaaccg actctatcaa
      661 gaaagcactt cgttcacccc tgctggatgt tgacatcacc ggcgcaaccg ccgcactcat
      721 caatgtaatc ggcggtccag acatgacgat gtttgaggct gagggtgttg tacaggaagt
      781 ttaccagcgg attgatcccg gagcacggat tatctgggga gttcaggttg acccggagat
      841 ggaaggccgc atgcggacaa tgcttattgt aacgggtgtt cagtccccac agatttatgg
      901 taagcaggag caaacatata cggcatcaca atcaagtcag gttcagaaat ccaagtttga
      961 gattgacttc ctgaggtgaa tatattggca gatgaaaaaa atactgaaaa gaaggcagca
     1021 tcctctgctg taaacgtaaa gatgccggag gtctccaagc agagtatctc cgagttcttc
     1081 cgcaagtacc tgcgtgtatt aaaacttgca cgcagaccga caaaggatga gttcttcaag
     1141 atctccgcag tcgccgcagc cggtattgcg gtaatcggag ttctcggttt catcatttac
     1201 ctggtcttcc actacctgct cccgtgaaac tatgactgat tcagagtttg gcaaccatta
     1261 ctacataatt aaaacaacat ccaagcacga gcggacggtt gcagataata tccgtgaggc
     1321 aatcgagaat aacatgacgg atgttgtcgt gacagctgta gtggttccgg aggatattaa
     1381 gggctatgtc tttgtggaaa gccccgagga acacgcgcgc attgaagagc tggtagagac
     1441 aattgctcat gcacgtgttg ttctgaaaag cgaaacgtcg atggaagaga tcaaacactt
     1501 ccttgtaccc aaaccggcag tctccggtat cgaagaagga acaattgttg aactgatcgc
     1561 agggccgttc aaaggtgaaa aggcagtggt caagcgtgta gaccacacca aagaagagat
     1621 caccgttgaa ctgtatgagt caattgttcc tattccaatc accgtgcgcg gcgataatgt
     1681 gcgcgtaatc gatcgcagcc acgagtaaat cccaatccat tttttagatc ctgaaatagg
     1741 tctgcgcgat ctg
//
LOCUS       DALTZZ010000240         3411 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_39375,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000240 DALTZZ010000000
VERSION     DALTZZ010000240.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39375"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(300..1292)
                     /gene="radA"
                     /locus_tag="O0X12_04630"
     CDS             complement(300..1292)
                     /gene="radA"
                     /locus_tag="O0X12_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadA"
                     /protein_id="HJK53471.1"
                     /translation="MTATDIEDIPGVGPATADRLREAGYITVESIATAAPVDLSEAAE
                     IGESTAKKIIKAAREMADIGGFKTGTDVLARRQEVLKLSTFVPEVDELLGGGIETQAI
                     TEFYGEFGSGKSQLVHQLAVNCQLPQEVGGLGGSCLYIDTENTFRPERIEQMVEGLEF
                     EKPLPEGYEIPGPETFLANIHVARAHSSDHQMLLIDSARDLANELKETDMPVKLIVVD
                     SLTGLFRAEYAGRGTLAGRQQKLNRHLHDLFKLVDELNAVAIVTNQVMSNPGVFFGDP
                     TKPIGGNIVGHSATFRLYLRKSKAGKRIARLVDSPNLPEGEATFMVETAGIKPC"
     gene            1440..1628
                     /locus_tag="O0X12_04635"
     CDS             1440..1628
                     /locus_tag="O0X12_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53472.1"
                     /translation="MTPAEDSSFKSALISAAGDLAKNPVIGPVIEQVAYDFLSSPECR
                     EAFAAYAKRRIKESIGVQ"
     gene            complement(1633..2916)
                     /locus_tag="O0X12_04640"
     CDS             complement(1633..2916)
                     /locus_tag="O0X12_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53473.1"
                     /translation="MHYALVSDLLTKEEFDERVEKKCEELGGAVDEICAAMLVVEELG
                     RSHIKIGEIKDTATALVSFFGKILEITPPREFRREGAEDAEPGVVASVVLGDPTGTVR
                     MTLWDAMAAAVSELELGSVLEVIAKPRPGYWNEVTCMALRPSQVTIVETKKPPRSETM
                     KIPLAAKVLHIGPVREVTRRDGSVSELQEILVGDPSGTARIITWEPELFADLDEGISV
                     SFAGLTRKEDEDSVEYIAGESVMITPHPQPIEVLTTDAGEVSEGQTSIVTGVVRSVSP
                     VRTFTTRRGTEARVKNIKLGSEKGYGYVNVACWNEAADTAVLAGDKIEVINAPAKLNK
                     YGDVELSVGRGSVLREREEEGVYLEFEGFVIPRSEGLTIDNGTEALLLVTDQPLTPAQ
                     RIRAGGICKSSRLYAESIETVPVSAADLRERLKNL"
     gene            complement(2920..>3411)
                     /locus_tag="O0X12_04645"
     CDS             complement(2920..>3411)
                     /locus_tag="O0X12_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HJK53474.1"
                     /translation="FVDACKEAGIAPAFWVEPGRWMVGESTILLTGVNSVKTVHKTFI
                     NVDAGFNLLIRPAMYDSWHEVIVANKADAPASVTATVTGPICETGDILAADRKLPETV
                     TGDVIAVLDAGAYGYAMSSQYNSRPRCPEVLVNGDKAELMRRAETYEDMTATVVYPSW
                     HRK"
BASE COUNT          754 a         1053 c          821 g          783 t
ORIGIN      
        1 acggcgagga ggaagtatcc ggcaagaaga agccggagcg gtcctgtgag ttgactttca
       61 tgttcctttt cttcgcacat ttgatcaaca gatcataatg ttccggatta aacatcatct
      121 cacgagtatc gtcaccactc atacaccaat acatccctca cgaaaaacat acatatgacc
      181 tgactgttag actcaaatat ttctctattt ctgtctgatc ccatcccagg gacgggcatt
      241 ttgttaccac tcggcttcta aaaatagtga ttttcggggc attgcccccg tagggttgtt
      301 taacagggtt tgatacctgc ggtctcaacc ataaaggttg cctctccctc tgggagattc
      361 ggactgtcca cgagccgggc aatacgctta cctgccttac tctttctgag atacagccgg
      421 aacgtcgccg agtggccgac gatgttaccg ccgatcggct ttgtcggatc accaaagaac
      481 actcctggat tggacataac ctgattggtc acaatggcaa ccgcgtttag ctcatcaaca
      541 agcttgaaga gatcatgcag atggcggttc agcttctgct ggcgtccagc aagcgtaccg
      601 cggcctgcat actcggcacg gaacaggccg gtcagggagt ccaccacgat cagcttcacc
      661 ggcatatcgg tctccttaag ttcgtttgcc agatcacgtg ccgagtcgat cagcagcatc
      721 tggtgatccg aactgtgggc acgggcaaca tgaatatttg caagaaacgt ctccggcccc
      781 ggaatctcat acccttccgg aagcggcttt tcaaactcaa ggccttctac catctgctcg
      841 atacgctccg gccggaacgt attctccgta tcgatgtaca gacagctacc gccaagaccg
      901 ccgacctcct ggggaagctg gcagttcacc gcaagctggt gcaccagctg ggactttccg
      961 gaaccaaact caccataaaa ctcggtgatt gcctgcgtct caattccgcc gcccagaagc
     1021 tcatccacct cgggaacaaa agtactgagc ttgagaacct cctgtctgcg tgcaagaaca
     1081 tcagtaccgg tcttaaaccc gccgatgtct gccatctcac gggccgcctt gatgatcttc
     1141 tttgctgttg actcaccaat ctcggcggcc tcggagagat ccaccggtgc agcggttgca
     1201 atgctttcaa cagtaatgta tcctgcctcg cgaaggcggt cggcggtcgc cggtccgact
     1261 ccgggaatgt cctcaatatc agttgctgtc atatcttctg tctcctttgt attcttgaga
     1321 tatactatta catcgacatt tgcggcatat aaaccccgga ccacgcgtac tgaaagtgcc
     1381 cccagctatg actgtcagca tccatacaga tttatccaaa ccctgcccat gatacattca
     1441 tgacacccgc ggaggactca tcctttaaat cagcactgat cagtgcagcc ggagatcttg
     1501 ccaaaaaccc ggtcatcggt cctgtcatcg aacaggtcgc ctacgacttc ctctcctctc
     1561 ccgaatgccg cgaagccttc gctgcctacg caaaacgcag aatcaaggaa agcatcgggg
     1621 ttcaataaaa gatcacagat tcttcagccg ttcccgcaga tcagcagccg acaccggaac
     1681 agtctcgata ctctccgcat acagccggga acttttacag atacctccgg cacgaatccg
     1741 ctgtgccgga gtaagcggct gatccgtgac gagaagcagg gcctccgttc cgttgtcgat
     1801 ggtcagcccc tcggaacggg ggataacaaa tccctcaaac tcgagataca ctccctcctc
     1861 ctcccgttcc cgcagaaccg aaccacgtcc gacggacaac tccacatccc cgtacttgtt
     1921 cagctttgcc ggagcgttaa tgacctcgat tttatccccg gcaagcacgg ctgtatccgc
     1981 cgcctcattc cagcaggcga cgttcacata cccgtatcct ttctcactgc cgagcttgat
     2041 gtttttcacc cgggcctcag tgccccggcg ggtcgtaaac gtccggaccg gagaaacgga
     2101 acgaaccaca ccggtcacaa tcgaggtctg accttcggaa acctcgcctg catcggttgt
     2161 gagaacctcg attggctgcg gatgcggcgt aatcatcacg ctttcgcctg caatatactc
     2221 caccgagtct tcatcctctt ttctggtcag cccggcaaaa ctcaccgaaa tcccttcatc
     2281 cagatccgca aacagttccg gttcccatgt aatgatccgt gccgtaccgg acggatcgcc
     2341 cacaagaatc tcctgaagtt cagacaccga tccgtcacgg cgggtcacct cacggacggg
     2401 acctatgtga agcactttgg ccgccagcgg aatcttcatc gtctcggatc gcggcggttt
     2461 ttttgtctcc acaatggtga cctggcttgg cctgagtgcc atacaggtca cctcgttcca
     2521 gtaaccgggc cgcggctttg cgatcacctc aagaaccgat cccagctcca gctctgagac
     2581 ggctgctgcc attgcatccc agagtgtcat tctcaccgtc ccggtcggat cgccgagaac
     2641 aacggatgcc acaactcccg gttctgcatc ctccgcacct tcacgcctga actcacgggg
     2701 cggagtaatc tcaagaattt ttccgaaaaa agagacaaga gcggtggcag tatctttgat
     2761 ctcaccgatc ttgatgtgac tgcgtcccag ctcttcaacg acaagcatag cggcgcagat
     2821 ctcatccact gctccgccaa gctcctcgca tttcttctct acccgctcgt caaactcctc
     2881 ttttgtcagc agatccgaga cgagcgcata gtgcatgggt tacttcctgt gccatgacgg
     2941 gtacacgacg gttgcggtca tgtcttcgta ggtttcggca cggcgcatca gctctgcctt
     3001 atcgccgttg acgagaacct ccgggcaccg cgggcggctg ttgtactgtg aggacatggc
     3061 gtacccgtat gctcccgcgt ccagaaccgc gatgacatcg ccggtcacgg tttcgggaag
     3121 ctttctgtct gcggcgagga tatcaccggt ctcacagatc ggtcccgtaa cggttgcggt
     3181 gacgcttgcc ggtgcatcgg ctttgtttgc aacaatcacc tcatgccagg agtcgtacat
     3241 cgccgggcgg atcaggaggt tgaatcctgc atccacgttg atgaaggtct tgtgcacggt
     3301 cttcaccgag tttacgccgg tgagaagaat ggtggactcg ccaaccatcc agcgtccggg
     3361 ctcaacccag aacgccggtg caattcctgc ttccttgcat gcgtccacaa a
//
LOCUS       DALTZZ010000241         6555 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_39438,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000241 DALTZZ010000000
VERSION     DALTZZ010000241.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39438"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            320..1369
                     /locus_tag="O0X12_04650"
     CDS             320..1369
                     /locus_tag="O0X12_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53475.1"
                     /translation="MSCKIVFVLSLVSFIIILNIIPVTALDVNITDEPEIIHISSPES
                     FVRLTLSDGTYLVMSEYQPGIDEYGVVYLYDISKEAISVVPMADSYSKAIVANKTIFF
                     RYTDGFYSYHPSSDVLKKLNVPIQPILTGFVTDGIHFITMWGDYWPRDLTFMMYDLQT
                     GEQSQIYPGGYPDPSGPMFLSGDYFVYDDSGMLVADPDKRYLHAYNISSGETITLPKE
                     DGYSQYLANVWGNTIVYELFSLLDNAYPDPPQYRIFNLKTMESASLLLPDGIHPSQVY
                     PPYALQSEWDQETGVTTVKLVRVDLPPIVHPTTESSVIEQNPAPPTTPAGSSIVLILG
                     LVAFILAGVLTIFWR"
     gene            1620..2261
                     /locus_tag="O0X12_04655"
     CDS             1620..2261
                     /locus_tag="O0X12_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53476.1"
                     /translation="MTSAEYLEVVWPQVYTKISPEDRETLTGFTHIWELQELDRTGQG
                     TGFGLTINEAIGRERFALMDAIEELEITEGEYQGITQPELFLDLSEETRKILSSMVRA
                     QRGSGSGIRSGTSISEGETHWFSKNVTSNVTRLAVDLQWENPQDIENNLSITIYSPDR
                     CVFGPFTIAEPLYANPQKMQIYSYISRPGGVAEGEWWYCVKGVKIHGVQNYTI"
     gene            2236..3027
                     /locus_tag="O0X12_04660"
     CDS             2236..3027
                     /locus_tag="O0X12_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HJK53477.1"
                     /translation="MEFRITLFNLIVAVLLLCLLSVPVSADNASVPGEMPRNFADVPS
                     WIIDSIGIKTPVEGGYTVNEPLSDNLGGFSDINRADAARDESDVPVWILVCAGVSGIA
                     VIVCVTVQFLPILIGRTREASPSPVRDRVFAAITDIPGSSAAVLREKTGIHRATLRYH
                     LSILERENRIISRRAGHIYHYFPNGSSLSYTDTIRMTLCQNPTTQNILSIIEKEPGIS
                     GTELAARAGITPGTLTWHLRRLEKNDLITISRVGRCMQIFPLDNT"
     gene            3136..4122
                     /locus_tag="O0X12_04665"
     CDS             3136..4122
                     /locus_tag="O0X12_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HJK53478.1"
                     /translation="MLEIYRSADASPIPSRIDTIQSGCWVRMIHPTEDEIAYVCTTLS
                     IPREDIITLLDDEESPRIEHDDGRTLIIVDTPYVDTDDTDPTVSHPAKTNGTTSYTTV
                     PAGFIVMQDIIITVSLRTNPVLQTFIDGKVKNFSTVKKTRFLLQFLYRNARLFVQHLR
                     QIDNLTSTIEHTLYKSTRNEELIEMLRISKSLVYITHALKANEAVLEKLYRFPTPSVR
                     MYEEDEDLLEDTIIENRQALEMAQTYSSTLGHIMDTCASMINNNVNSIMKLFTVVTIL
                     LTIPTMIASFLGMNVIIPGYLSENPFAFHLLIVGSLSISATLLWLLFRRKII"
     gene            complement(4192..4911)
                     /locus_tag="O0X12_04670"
     CDS             complement(4192..4911)
                     /locus_tag="O0X12_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJK53479.1"
                     /translation="MEFAEIETITQGELEIMNPISEEKLLRAGKMAGLCPGKRVLDIG
                     CGNGTALSLWYKTFGITGTGIEIRPSSAARARNTLAGTPIEIRCVDASVLAPKEPYDV
                     VTALGTSFIFGGAEKALANLAEYADENGSVVLIGDRFWRTDRVPPEFAREWTEVPTAY
                     ELAVTARELGFTLTGMITASADEWDAYESAIWNSALAYIDLHENEPAADELAAYLEQI
                     QDEYLAYGREYIGWGLFVFRQ"
     gene            5050..6012
                     /locus_tag="O0X12_04675"
     CDS             5050..6012
                     /locus_tag="O0X12_04675"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HJK53480.1"
                     /translation="MDLKKVSGSVVLCSLLVLLAACCYGILSTIVKIAVGNGVGIPVI
                     LVGKFFYGLVILFVLVVLVHFLFPRKDKPPKSTLPPWKRGVILFVTGIVMCLVTVCYF
                     YSVSYLPVSVAVILLFQFSWMGVVIEAVANRRMPSRNQLIAVVIIFVGTILAGGTTGF
                     GMEINPFGVVLGLLAAFFYAMFVFLSGRVEPSMTPMNRSFLIALSGFIFVCGLIACID
                     SPAAIPAGIFSSDSLIYGVALGLFGVALPILFLAIGAPRISTGMATILNAAELPVEVL
                     AAAVVLVEAVSGLQWVGVLVILIGIAFPHLMEKRISSGLAEDAA"
     gene            complement(6061..>6555)
                     /locus_tag="O0X12_04680"
     CDS             complement(6061..>6555)
                     /locus_tag="O0X12_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HJK53481.1"
                     /translation="GSLLTGADGNAGEVGHIVVDTRYNILGGCGGTGHWEAYASGTGI
                     PKFFHAWYAEKCDGRCPAKRDITAGQILRAAEIGSPLCCEFVDVLAKINGRGLSAVIN
                     AYNPDLIILDGPIVRNYPSLVAGKMTAAIDRYLRMPEIRITTLEGRAPLIGAAAAAFR
                     ITNE"
BASE COUNT         1647 a         1622 c         1474 g         1812 t
ORIGIN      
        1 cttaatatct ctgaagctga gtatatgcag atcgtgtggc ctgaattatg gacagacatg
       61 agtactgcga cgaagacaca ccttgcaact cttccaaaca tgcattctac ggctatgaag
      121 ggaataatgg ttggggggtt ttagtcgctc ttgttcattg atggttaccg ttggaactac
      181 ttttgaaatt gatcgaatgt aatttaaatg aggatagtga gataaccgcc ttatttccag
      241 gagagagtga gatcaagaat ttgaacatgg ttgaacatct tcccgctgaa acacttagag
      301 taaattggat tgtctgagaa tgtcatgtaa aatagtattt gtactttcat tagtgtcgtt
      361 tataatcata ttgaacatta ttccagttac tgctcttgac gtgaacataa ctgacgagcc
      421 tgagatcatt cacatatcat ctccagaatc ttttgtacga ctaaccttat ccgatgggac
      481 ctatcttgta atgtcggagt atcaaccagg aattgacgaa tatggggtgg tttatcttta
      541 tgatatttcc aaagaggcga tctctgtagt ccctatggct gattcatatt ccaaagctat
      601 tgttgccaac aaaaccatct tcttcagata tactgatgga ttctattcgt accacccctc
      661 atcagatgta ttaaaaaaac tgaatgtacc tatacaaccg atactgacgg gttttgttac
      721 ggacggaatt cattttatca cgatgtgggg agactactgg ccaagggatc ttaccttcat
      781 gatgtatgat cttcaaacag gagaacagag tcagatatat ccaggaggat atcccgatcc
      841 gagcggcccg atgtttctct ctggagatta tttcgtgtat gatgattctg gaatgcttgt
      901 agcagatcca gataaacgat atctccatgc ttacaacatc tcatcaggag aaacgatcac
      961 tctcccaaag gaagatggat acagtcagta tcttgcaaac gtctggggaa acaccattgt
     1021 ctatgaattg ttcagtcttt tggataatgc atatccagat ccaccgcagt accgtatatt
     1081 caatctgaaa accatggagt cggcatcttt gctccttccg gatggaattc atccatctca
     1141 ggtctatccc ccttatgccc ttcagtctga atgggatcaa gaaactggtg taactacagt
     1201 aaaacttgtt agagttgatc tccctccgat tgtccacccg actactgaat catcagtaat
     1261 agagcagaat cccgctccgc caacaactcc ggctggttca tcgattgttc tgatccttgg
     1321 cctggtagca tttattctgg ctggcgttct tactattttc tggagataga aataatcaga
     1381 atccctacca cctttatttt gacgtaactg ccagaaatat tatatacatc tctctacgaa
     1441 tgaaagcaca tcgaacaaag gagatgctgt caatcaaaca catattcaga atcttttatc
     1501 tggcaggcat tgtgtttctg ctactggcaa tccctgttct cgcagcaact cctgctgaag
     1561 aaaactatga gcggatccaa aaaagccttg tcctgatgga gcagtacaac ggtaccgaga
     1621 tgacctctgc tgaatatctt gaagttgtct ggccacaggt gtacacaaaa atatcccccg
     1681 aggacagaga aacactcact ggtttcacgc atatttggga gttacaagaa cttgatagaa
     1741 ctggtcaagg aactggattt ggcctcacca tcaatgaagc aattggtagg gagcgcttcg
     1801 ctctgatgga tgctattgaa gaactggaga taaccgaggg tgaatatcag ggaatcaccc
     1861 aaccggaact ctttctagat ctttctgaag aaacacgcaa aatactcagt agcatggtac
     1921 gtgcccaacg cggttcgggt tctggtatcc gtagtggtac atctatcagt gaaggagaga
     1981 cacactggtt ttccaaaaat gtcacctcaa atgtaacccg acttgctgtt gatctccagt
     2041 gggagaatcc gcaggacatt gaaaacaacc tcagtattac catatactcc ccggacaggt
     2101 gtgtttttgg tcccttcacc atagccgaac cactttacgc caatccccag aaaatgcaaa
     2161 tttactccta tatatcacgt ccaggtggtg ttgcagaagg ggagtggtgg tactgtgtca
     2221 aaggagtaaa gatccatgga gttcagaatt acactattta atctgattgt tgcggtactt
     2281 ctcctctgcc ttctttccgt accggtttca gctgataatg cttcagtacc tggtgaaatg
     2341 cctcgaaact ttgcggatgt cccctcgtgg atcattgaca gtattggcat caaaactccg
     2401 gttgaaggtg gttatactgt caacgaacct ctctctgata accttggagg tttctcggac
     2461 atcaatcgtg cagatgctgc tcgtgatgag tctgacgttc cggtatggat ccttgtatgt
     2521 gcaggagttt ctggcattgc agttatagta tgcgttactg ttcagttctt gcctatcctg
     2581 atcggaagaa cccgtgaagc atcgccaagt cctgttcgag accgcgtgtt tgcagctatc
     2641 actgatatac ccggcagctc tgctgcagtt cttcgagaaa agactggaat ccatcgtgcg
     2701 acactccggt atcatctttc gattctcgaa agggaaaatc gaatcatctc ccgcagagca
     2761 ggacacatct accattattt cccgaatgga tcctctctta gctatactga taccatccgc
     2821 atgacactgt gccagaatcc aaccacacaa aacattctct caatcatcga aaaagagccc
     2881 gggatctccg gtactgaact tgctgctcgt gctggtatca ccccgggaac ccttacctgg
     2941 caccttagac ggcttgaaaa gaacgatctt atcacgatat ctcgtgtagg acggtgcatg
     3001 cagatctttc ccctggataa cacctgatgc tatctcacta ttttttcaag gttttcagta
     3061 cttcccgata ctctatatat tctccataca aacaaatcaa gtaacgtcgg cacaaatact
     3121 gccgaggaga tccgaatgct ggaaatctac cgatccgctg acgcttcacc aattccgtcc
     3181 agaatcgaca ccattcaaag cggctgctgg gttcgcatga tacatccaac cgaagacgag
     3241 attgcctacg tctgtacaac actgtccatt ccccgggaag acatcataac cctgcttgat
     3301 gacgaagaaa gtccgcgtat tgaacacgat gatggtcgaa ctcttatcat cgtggacaca
     3361 ccgtacgtgg acactgacga cactgacccg actgtatcac atcccgcaaa aacaaacggc
     3421 accacgagct acactacagt tccggctggc tttatcgtca tgcaggacat cattattacc
     3481 gtctctcttc ggacaaaccc ggttctccag accttcatcg atggaaaagt aaaaaatttt
     3541 tccacggtga aaaaaaccag attccttctt cagtttctct accgcaacgc tcggctcttc
     3601 gttcagcatc tccgtcagat cgacaacctg accagcacca ttgaacacac actctacaaa
     3661 tccaccagaa acgaagagct catcgagatg cttcgtatat caaaatccct tgtctatatc
     3721 acacacgccc tgaaggcgaa cgaagcggta ctagaaaaat tgtatcggtt cccaacgccg
     3781 tctgtccgga tgtatgaaga ggacgaggat cttctcgagg acaccatcat cgaaaaccgg
     3841 caggcactcg aaatggccca gacctacagc agtacccttg gccatattat ggacacctgt
     3901 gcctccatga tcaacaacaa cgtcaacagc atcatgaagc tgttcaccgt tgtaaccatt
     3961 ctcctgacca ttccgacgat gattgcaagc ttcctcggca tgaacgttat catccctgga
     4021 tatctctctg aaaatccatt cgcatttcat cttctcatcg tcggatcact cagcatctcc
     4081 gcaactctct tgtggttgct gttccggaga aaaatcatct gaaaaatagt ttgatgggaa
     4141 aatgatccta agttttttcg aatactacag aatcagcaat tcctgatccg gttactgtcg
     4201 gaacacgaac aatccccagc cgatgtactc acgtccgtag gcgagatact cgtcctgaat
     4261 ctgttcgaga taggctgcaa gctcatcggc tgccggttcg ttctcgtgca gatcaatgta
     4321 tgcaagggca ctgttccaga tggcggattc gtaagcatcc cactcatctg ctgatgcggt
     4381 gatcattccg gtgagagtaa acccaagctc ccgtgcagtt accgcaagtt cgtaggcggt
     4441 cgggacttcg gtccactcac gggcaaactc cggcgggacg cgatcggttc tccagaaccg
     4501 atctccgata agaacaacac tgccgttctc atcggcgtac tccgcaaggt tggcaagcgc
     4561 tttttctgcc ccgccaaaga taaaggaggt accaagtgcc gtaacaacgt cgtagggctc
     4621 tttgggtgca agtacagagg catccacgca gcggatctct atcggtgttc ccgcaagagt
     4681 gtttcttgcg cgggctgcgg aggacggacg aatctctata ccggttccgg tgatgccgaa
     4741 ggttttgtac cagagggaca gtgcagttcc gttgccgcat ccgatatcga gaacacgttt
     4801 tccggggcaa agccctgcca tctttccggc acgcagaagt ttttcctcgg agatcgggtt
     4861 catgatctca agttctccct gggtaattgt ctcaatttcc gcaaactcca tagtacacaa
     4921 tagatctttc ctgaaacaaa agtagttttt cacatttttt gttgctcatc gatccagttt
     4981 ttctgcgtta tcgcaactga atggataatt attataggat actccgacct accatagatt
     5041 cagattgata tggatctgaa aaaagtatcc ggatcagttg tgctgtgctc gcttcttgtg
     5101 ctgcttgccg cctgctgtta cggtatttta tcaactatcg taaagattgc cgtcggaaac
     5161 ggtgtgggta ttccggttat tctggttggc aagttcttct acggtctggt tattctgttt
     5221 gtgttagtgg ttcttgtcca tttcctgttc ccaagaaagg acaaaccgcc aaagagtact
     5281 cttcctccat ggaaacgcgg tgtgatcctt tttgttaccg gtattgtgat gtgtctggta
     5341 acggtctgtt acttctactc ggtaagttac ctgccggttt cggtcgcagt aattctgttg
     5401 ttccagttct cctggatggg tgtcgtgatc gaagcagttg caaaccgcag aatgccgtca
     5461 cgcaatcagc tgattgctgt tgtgattatc tttgttggta cgattcttgc cgggggtacg
     5521 accggttttg gtatggagat caacccgttc ggtgtggtac tcggtctgct tgcagcattc
     5581 ttctatgcaa tgtttgtgtt cctgagcgga cgcgtcgaac cgtcgatgac accgatgaac
     5641 cggagtttcc tgatagctct ctccggattt atcttcgttt gtggtctcat cgcctgtatc
     5701 gacagccccg cagccattcc cgcaggaatc ttttcgagcg actcactgat ctacggtgtt
     5761 gctctcggtc tgtttggtgt tgcactgccg attctgttcc ttgcaattgg tgccccgcgt
     5821 atcagtactg gtatggcaac gatcctgaat gcagccgagc tgccggttga ggttcttgca
     5881 gctgcggtgg ttctggttga ggctgtttcc ggtcttcagt gggtaggtgt gcttgtaatc
     5941 ctgatcggta ttgcattccc gcacctcatg gaaaagagaa tttcaagcgg ccttgcagag
     6001 gacgctgcat aatttttatt ttttaaaaag agaactcagt tttacaaact ctttgggcaa
     6061 tcattcgttg gtgatccgaa atgctgctgc ggcagcaccg atcagcggag ctctgccctc
     6121 cagtgtggta atcctaattt ccggcattcg cagatagcga tcaatggctg cggtcatctt
     6181 tccggcgacg agtgacggat agtttcggac gatcggtccg tcaaggatga tcagatccgg
     6241 attgtatgca ttgatcaccg cagacaaacc gcgaccgttg atctttgcaa gtacatccac
     6301 gaactcacag cagagaggac taccgatctc tgccgcccgc aaaatctgtc ctgcggtgat
     6361 atcccttttt gccgggcagc gtccgtcaca cttttctgca taccaggcat ggaaaaattt
     6421 cggaatcccg gtgccgcttg catatgcttc ccagtggccg gtaccgccgc agccgccgag
     6481 gatgttgtat ctggtatcaa caacgatgtg tcctacctcc ccggcgttcc catctgctcc
     6541 tgtaagcagg gatcc
//
LOCUS       DALTZZ010000242         2208 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_39681,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000242 DALTZZ010000000
VERSION     DALTZZ010000242.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39681"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            212..766
                     /locus_tag="O0X12_04685"
     CDS             212..766
                     /locus_tag="O0X12_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HJK53482.1"
                     /translation="MYGNEKRARLLAESAVFIALIAVGGWISIPILIIPFTLQNIFIL
                     LAAAVMKRYAVIPVGLYVLFGSLGLPIFHNGTAGLGVLLGPTGGFLIGFVIMGAVAGW
                     LFEKETIRYDILACAAFVTGLYVFGTAWFMISTGAELTAALLACAVPYIPGDIIKCVI
                     VEAVILRLRSAKNKTSQTNNDIHS"
     gene            750..1349
                     /locus_tag="O0X12_04690"
     CDS             750..1349
                     /locus_tag="O0X12_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK53483.1"
                     /translation="MISIADLRYGIVDIPHLSIPEGISAVTGDNGAGKTTLLRLAAGM
                     LLPETGTITINGTAPRACEVGCVSEFPDRHLIFSVVRDEIASPLRFAHRKPAEIDDAV
                     TSITDRLGIAHLLDRECRTLSGGEKMLVGIATALIAKPVLITLDEPDSHLDPQTTDEL
                     FQIIRNSGCPYVLWSTHSRSVQERADHRITLVYRKVSDL"
     gene            1346..1912
                     /locus_tag="O0X12_04695"
     CDS             1346..1912
                     /locus_tag="O0X12_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJK53484.1"
                     /translation="MKLIFDAVRFQRDHFTLSVDATFSCGIHLISGRIGTGKSTLALA
                     AAGILSPNSGEILYEEIRDTPLLLMQFPEYQVTGTTVSEEITSWNIIGNEEPFHRLNT
                     GPSSRDPLTLSRGELRRLELACILSRESDLLILDEPYASLDQAAKPELTELLEQRTGI
                     TLIFSHETEYLPTTAEHWTIQEGKLYRD"
     gene            1905..>2208
                     /locus_tag="O0X12_04700"
     CDS             1905..>2208
                     /locus_tag="O0X12_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53485.1"
                     /translation="MTDIRIRLIVVILLSLSAFTGVLGTLLAAACWIVFCAEETFSHN
                     SWKLFMPSAVLAAGFPGLILYLSGGDGGIYAAKIFVIFCLAFWLGVSHKPGEFLDLG"
BASE COUNT          550 a          600 c          513 g          545 t
ORIGIN      
        1 acattgatga acacggggca ctgctggtgc accgcgacgg cggagaggtc gaacgcgtga
       61 ttgcgggcga ctgcttcatg ctctaatcat tttctttttc agagtttacg catcttgtga
      121 aaactccttg gtaccatata cactgatttt ttatcccgtt tccaatcagc acccttattg
      181 tttaccaaaa tcaaacaagt agtttacaat catgtacggt aatgaaaaac gggcacgttt
      241 gctcgcagag tcagcggtat tcatcgccct catcgcagtt ggcggatgga tatccattcc
      301 aattctgata attcccttta ccctgcaaaa tatattcata ctgcttgccg cagccgtaat
      361 gaagcggtat gcagtaattc cggtcggact ctatgtcctc tttggatcac ttggtctgcc
      421 gatcttccat aacggaacag caggacttgg agtacttctc ggaccgaccg gaggatttct
      481 gataggattt gtgataatgg gtgcagttgc aggatggctg tttgaaaaag aaaccatccg
      541 ctatgacata ctcgcctgcg ctgcatttgt cactggcctt tatgtcttcg gaaccgcctg
      601 gtttatgatc tcgaccggag cagaacttac ggcagccctc ctcgcgtgtg ctgtaccgta
      661 cattccagga gacatcatca aatgcgtaat tgtcgaagca gtaattctcc ggctacgcag
      721 cgcaaaaaac aaaacatcac agacgaacaa tgatatccat agctgatctc aggtacggca
      781 ttgtggatat tccccacctt tccatcccag agggaatctc cgctgttacc ggagacaatg
      841 gtgcaggaaa aaccacactt ctccgtcttg ctgcaggaat gctgctgccg gaaacaggaa
      901 caatcaccat caacgggact gcgccgcgag catgtgaagt cggttgtgtc tccgagtttc
      961 cggaccggca tctgatcttt tcagtagtac gtgacgagat cgcatctccc ctccggttcg
     1021 cacaccgcaa acccgcagaa atagacgatg cagttacctc gatcacggac cggcttggaa
     1081 ttgcacatct gctggacaga gagtgccgga cactttccgg cggggaaaag atgcttgtag
     1141 gtattgcaac agcactcatc gcaaaaccgg tactgatcac gcttgacgag cccgattccc
     1201 atcttgatcc gcagaccaca gatgaactgt ttcagatcat cagaaacagc ggctgcccgt
     1261 atgtactctg gagtacacac tcacgcagcg tccaagaaag agcagatcac cggatcacac
     1321 tcgtgtacag gaaggtgagt gatctgtgaa acttattttt gatgcagtac ggtttcagcg
     1381 ggatcacttt acactatccg tggatgcaac attttcctgt ggcattcatc tcatctcagg
     1441 gaggattggt accggaaaat ccacactcgc acttgcagca gcaggaattc tctctccaaa
     1501 ctcaggagag atcctatacg aagaaatcag ggatacgccg cttctattga tgcagtttcc
     1561 cgagtatcag gtaaccggaa ccacggtatc tgaagagatt acctcctgga acattattgg
     1621 aaacgaggaa ccctttcatc gcctgaacac cggaccatcc tcccgtgatc cactcacctt
     1681 atcccgcgga gaacttcgcc ggctggagct cgcctgtatc ctctcccggg aaagtgatct
     1741 cctgatactc gatgaaccct acgcatcact ggatcaggcc gcaaaaccgg aactcaccga
     1801 actgcttgaa cagcgcaccg gcatcactct catattttct catgagaccg agtatctgcc
     1861 aacaacagcg gaacactgga caattcagga gggaaaactg taccgtgact gacatccgca
     1921 tacgcctcat cgtagtgatt ctcctctccc tatctgcatt caccggagta cttggaacac
     1981 tccttgccgc ggcctgctgg atagtattct gtgcagaaga aacattctct cacaactcct
     2041 ggaaactgtt catgccctcc gcagttcttg ccgcaggatt tccgggactc atcctgtacc
     2101 tttccggagg tgacggcggg atctatgccg caaagatctt tgtcatattc tgcctcgcat
     2161 tctggctggg tgtatcacac aaaccgggag agtttcttga tctcgggg
//
LOCUS       DALTZZ010000243         1791 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_39720,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000243 DALTZZ010000000
VERSION     DALTZZ010000243.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39720"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="O0X12_04705"
     CDS             complement(<1..232)
                     /locus_tag="O0X12_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJK53486.1"
                     /translation="MLSMIGIIIGVLAITSLGMMGGAFSAEMTSLLSSSTNSLTIMSV
                     EEKTADGIPVTGLSSKDLRDIEAAIRSATSDYI"
     gene            complement(327..1007)
                     /locus_tag="O0X12_04710"
     CDS             complement(327..1007)
                     /locus_tag="O0X12_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK53487.1"
                     /translation="MSEVPVVELVDVYKVYHMPAGDVHALNGVTFSVMKGEFVAIMGP
                     SGSGKSTLMNMIGCLDVPTAGDMYIDGRNVKEMTDDELTIHRRDRIGFIFQKFNLIGL
                     LTAYENVEYPMILKYGKRDDTGRPKELLASVGIDEAKMTHTPFELSGGQQQRVAIARA
                     LANDPALLLCDEPTGNLDSKMSEQIMALLQDLNRKGRTIVMVTHDPHTATYANRTIVI
                     RDGEIVNE"
     gene            complement(1011..>1791)
                     /locus_tag="O0X12_04715"
     CDS             complement(1011..>1791)
                     /locus_tag="O0X12_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53488.1"
                     /translation="AVRVDDTQLSVALIDQPDAFGQSRNEKVTIRVGNPRANSLNGIQ
                     ITVSGDGVSSKQTAYFVGKLESDQYIDANLTVVVDKPTDISFDVSYRNGQNLHTNSVQ
                     IPVELGERKVRAEMILNNIVVSNKGTYYQITGDVNNAGLEDAKSVMVTTAKATPTAPM
                     ETYVVGSLEPDGIAEFDVTFVNPESNIIDLVVSYKDSDGNPYSTTVPVTLQGTIVSDS
                     SSSPGDGSPLGIIVAAVIILLIAGCAAVAWRRGFLDDIRKK"
BASE COUNT          487 a          417 c          412 g          475 t
ORIGIN      
        1 ggatataatc agaggttgct gaacgaattg ctgcttcaat gtcacgcaga tcttttgagg
       61 ataaaccggt cacaggaata ccatccgccg ttttctcctc cactgacatg atcgtcagtg
      121 agttagtaga tgaggagaga agcgaggtca tctctgcgga aaatgcaccg cccatcattc
      181 caagagaggt gatcgcaaga acaccaatga tgattcctat catcgagagc atggatcgca
      241 ataagttcag tcgaagattc ctgacagaca gctcaaaaat gatgggagag aaaatcttac
      301 tgaaagatga ggtgttttga ttctttttac tcattcacaa tttctccgtc acggatgaca
      361 atcgtcctat tcgcataggt tgcagtatgc ggatcatgag ttaccatgac aattgttctc
      421 ccttttcggt tcagatcctg taataaggcc ataatctgtt cactcatttt tgagtcaagg
      481 tttccggtgg gttcatcaca gagaagaaga gcaggatcgt ttgccaaagc acgtgcaatg
      541 gccacacgct gctgctgacc accggagagc tcaaacggcg tatgagtcat cttggcttca
      601 tcgatgccga cacttgccaa tagttccttt ggcctgccgg tatcatcacg tttgccgtat
      661 ttgaggatca ttgggtactc aacattttca taggcggtca gaagaccgat caaattgaat
      721 ttctgaaaga tgaatccaat acgatcacgg cgatggatgg taagctcatc gtcggtcatc
      781 tctttaacat tacgaccgtc aatgtacatg tcacccgctg ttgggacatc aaggcaacca
      841 atcatattca taagagtaga ctttcctgag ccagatggac ccataatagc tacaaactct
      901 cctttcatta ctgaaaatgt gacaccgttt aaggcatgta catcacctgc tggcatgtga
      961 tagaccttat agacgtcaac caattctaca accggtactt cagacatggt ttattttttc
     1021 cttatatcat caagaaaccc gcgacgccat gcaactgctg cacatccagc tatcagaagg
     1081 atgatcactg cagctacaat gataccaaga ggcgagccat caccaggcga gctactgctg
     1141 tcagagacaa tggtaccctg aagggttact ggaacagtag tactgtacgg gttaccgtct
     1201 gaatctttat aggaaacgac aagatcaatg atattggatt ctggattgac gaacgtaaca
     1261 tcaaactctg caattccgtc cggctcaagg gatccaacaa cataggtctc catcggggca
     1321 gtaggtgttg ctttggctgt ggtaaccatg acggattttg cgtcctccag tcctgcgttg
     1381 ttgacatccc cggtgatctg ataataggtt cctttattag agacgacgat attattcagg
     1441 atcatctctg cacggacttt gcgttctccc agttcaaccg ggatctgaac agagtttgtg
     1501 tgcagattct ggccattgcg gtaacttaca tcaaaggaga tatccgttgg tttgtccacg
     1561 acaacggtaa ggtttgcatc aatatactga tccgattcaa gtttacctac gaagtaggcg
     1621 gtctgcttgg aagagacgcc atcaccgcta acagtaatct ggatgccgtt taagctgttg
     1681 gcacgcggat tgccgacacg gatagtgacc ttttcatttc ggctctgacc aaacgcatca
     1741 ggctgatcaa tcagagcaac tgagagctgg gtatcatcaa cgcggacggc a
//
LOCUS       DALTZZ010000244         4416 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_39993,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000244 DALTZZ010000000
VERSION     DALTZZ010000244.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39993"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(76..246)
                     /locus_tag="O0X12_04720"
     CDS             complement(76..246)
                     /locus_tag="O0X12_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HJK53489.1"
                     /translation="MAAKDNGKVQQKKYGRGANQCQLCGRKQGLVRRYNIYFCRQCFR
                     EWAPTMGFKKMN"
     gene            complement(248..763)
                     /locus_tag="O0X12_04725"
     CDS             complement(248..763)
                     /locus_tag="O0X12_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HJK53490.1"
                     /translation="MSDMQKPYVAKVVVHMGVGEAGERLVNAENIMSDLTQGSKPIRS
                     YAKNTLPAFGVRRGQPIGCKVTLRGEKAMTFLTTALKTYAVENVLHARQFDATGNFGF
                     GIEEHTDFPGQAYDPKIGIYGMDIIAVIEKKGTRTARRKIQQKKLNSKLHVTREESMA
                     FVTETFGIEVE"
     gene            complement(760..1479)
                     /locus_tag="O0X12_04730"
     CDS             complement(760..1479)
                     /locus_tag="O0X12_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4e"
                     /protein_id="HJK53491.1"
                     /translation="MTRTKRMTAPDAWQISRKESKYVVSTAHGPHNGSALPIGIWIRD
                     HMNLAQNTKEVKKILHDRNVVLNGNVVTDEHIGIDVFDIISFPKINKHYMILVDAKGR
                     HTEYEISPEAAKVQLVKVANKTTLVGGKTQINLTSGANFIGDNSYKGKDSLVIGLVGD
                     ERFVVQQHFPFAVGNMAVIIGGQHTMKTGKIVEIQIQQSSLPNRVILEDANGEKFETI
                     EDYVYMIGTSESFLSTWGVDA"
     gene            complement(1479..1844)
                     /gene="rplX"
                     /locus_tag="O0X12_04735"
     CDS             complement(1479..1844)
                     /gene="rplX"
                     /locus_tag="O0X12_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HJK53492.1"
                     /translation="MARIASIQPRKQRKFRYNAPIHVRGAFLHATLADDLRKKYGKRS
                     FRVVTGDTVKVLRGEFAGTEAVVDKVDVKNTKVLVHGVVVKKANGEDVPRPIDPSKVM
                     ITKLNLKDAKRVARLEVKA"
     gene            complement(1856..2254)
                     /locus_tag="O0X12_04740"
     CDS             complement(1856..2254)
                     /locus_tag="O0X12_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HJK53493.1"
                     /translation="MRGLTSKIPRALQTGSKMVCADNTGARVVQIVSVFGYHGVKNRQ
                     PKLGIGDLATVSVKKGTPDMKRKLVKAVVVRQKKEFRRPNGLRVSFEENAMILLNDNG
                     DPRGTDIKGPVAREVAERFPKVGSMATIII"
     gene            complement(2251..2577)
                     /locus_tag="O0X12_04745"
     CDS             complement(2251..2577)
                     /locus_tag="O0X12_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HJK53494.1"
                     /translation="MAKNIGLNVAVPEKDCDDVNCPFHGSLPVRGQVITGKVVSERMQ
                     GTVVVERNYLHFVRKYDRYEKRSSRIHAHMAPCLDARIGDEVKIAECRPLNKTTSYVV
                     VEVTRA"
     gene            complement(2931..3131)
                     /gene="rpmC"
                     /locus_tag="O0X12_04750"
     CDS             complement(2931..3131)
                     /gene="rpmC"
                     /locus_tag="O0X12_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HJK53495.1"
                     /translation="MAIFRAKEVAQFSDAELVENEQKLRNELIQQYGKVSAGGAPENP
                     GKIREIRRTIARIKTEQTKRQA"
     gene            complement(3137..3838)
                     /locus_tag="O0X12_04755"
     CDS             complement(3137..3838)
                     /locus_tag="O0X12_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HJK53496.1"
                     /translation="MTIEKKFVADGVRKVRVEQYLNKELKRAGYGGMDIVRTPVGTQV
                     TIFAEKPGIVIGKGGRLVRQITTDLTSVYGIDSPQVEVQQVANPNLNAQIMAERLANA
                     LERGWYFRKAGTSVIRRVMDSGALGCEVIIAGKLTGARARVQKFVEGYIKHSGEPAES
                     VVETGYATAVKKLGIIGVQVKIVPPGAVLPDHFEIRPDANPAPAQVAEADVFDEFDAE
                     LANEPELESEFPEEA"
     gene            complement(3841..4314)
                     /locus_tag="O0X12_04760"
     CDS             complement(3841..4314)
                     /locus_tag="O0X12_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HJK53497.1"
                     /translation="MARTEYSNKLTGDNIARAKANELGCSPKHAVEIAHLVRNMMADD
                     AVAYLEQVIALKRAVPFHRYARNVGHQKSLNGKTFGTAAGRYPVKAAAEYIRLIRSAQ
                     KNAEYAGLAPEKMVIVHAAANKGRCMKAIFPRAMGRATPKHRDTVNIELILREVQ"
     gene            complement(4320..>4416)
                     /gene="rps19p"
                     /locus_tag="O0X12_04765"
     CDS             complement(4320..>4416)
                     /gene="rps19p"
                     /locus_tag="O0X12_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843411.1"
                     /note="protein S19 forms a complex with S13 that binds
                     strongly to the 16S ribosomal RNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HJK53498.1"
                     /translation="YFGEFALTRKKVSHGSAGIGATRSSKYVPLK"
BASE COUNT          975 a         1207 c         1055 g         1179 t
ORIGIN      
        1 cggtgtcacc ggcattcttg atagcgctca ttgcgtctgc aatcggattc tgttttgtca
       61 ttctccgttc acctcttagt tcatcttctt aaagcccatc gtgggtgccc actcacggaa
      121 gcactgacgg cagaagtaaa tattatatct gcgcacaagt ccctgcttgc gtccgcaaag
      181 ctggcactgg tttgcaccac gaccatactt tttctgctgg actttgccgt tgtcctttgc
      241 tgccatatta ctccacctca atgccgaagg tctcagtcac aaatgccatg gactcctcac
      301 gggtcacatg cagtttgctg ttgagcttct tctgctggat ctttctgcgt gccgttctcg
      361 tacctttctt ctcgataact gcgatgatat ccataccata gataccgatc ttcgggtcat
      421 atgcctgacc cgggaagtcc gtgtgttcct caatgccgaa accgaagttt ccggttgcat
      481 caaactgacg tgcgtgaaga acattctcca ctgcataggt ctttaatgcg gttgtaagga
      541 acgtcattgc cttctcgccg cgaagggtaa ctttacagcc aatcggctga ccacgacgaa
      601 caccaaaagc cggaagtgtg ttctttgcat aagaacgaat cggcttggaa ccctgagtaa
      661 gatcggacat gatgttttca gcgttgacaa gccgctcgcc tgcctcgccc acacccatgt
      721 ggacaacaac ctttgccacg tacggtttct gcatatcgct catgcgtcaa caccccaggt
      781 ggaaaggaag gactcagagg ttccgatcat atagacatag tcctcaatgg tctcgaactt
      841 ctcaccgttt gcatcctcaa ggatgacacg gttcggaaga gaagactgct gaatctggat
      901 ctcaacgatc tttccggtct tcatcgtgtg ctgaccgcca atgatgacag ccatgtttcc
      961 aactgcgaac gggaagtgct gttggacaac aaatctctca tcgccaacaa gaccgatcac
     1021 aagggagtcc ttgcctttgt aagagttgtc gccgataaag tttgcaccgg acgtcaggtt
     1081 gatctgtgtc ttgccgccga caagagtagt cttgtttgca accttgacca gctgaacctt
     1141 tgctgcttcc ggagagatct catactcggt gtggcgtccc tttgcatcaa caaggatcat
     1201 atagtgcttg ttaatcttcg ggaagctgat aatgtcaaag acatcaatac cgatgtgctc
     1261 atcagttacc acgttgccgt tgagaacaac attacggtcg tgaaggatct tcttgacctc
     1321 tttagtgttc tgggcgaggt tcatgtggtc acgaatccag atgccaatcg ggagagcaga
     1381 tccattgtgc ggaccgtgag cagtgcttac gacatacttg ctttccttgc gggagatctg
     1441 ccacgcatcc ggtgcggtca tacgctttgt acgagtcatt atgccttcac ctcaagacgt
     1501 gcaacgcgtt ttgcgtcctt caggttcagt ttggtgatca tcactttgga agggtcaatc
     1561 ggtcttggga catcttcacc gtttgctttc ttcacaacaa caccgtgaac aagaaccttt
     1621 gtgttcttca catcgacctt gtcaacaaca gcttcagtgc ctgcgaactc accgcggagc
     1681 actttaacgg tgtcaccggt gacaacacgg aaactgcgct tgccatattt cttacggaga
     1741 tcatcagcga gagttgcgtg taaaaatgca ccgcgaacat gaattggtgc gttgtaccgg
     1801 aactttctct gttttctcgg ctgaatgctt gcaatacgtg ccatgtttct tcaccttaga
     1861 taatgatcgt tgccatggat ccgaccttcg ggaaccgctc tgcaacttca cgtgcaaccg
     1921 ggcccttgat atcagtacca cgcgggtcac cgttatcatt caggaggatc attgcgttct
     1981 cttcaaagga aacacgcaga ccattcggac ggcggaattc cttcttctga cggacaacaa
     2041 ctgccttcac aagctttctc ttcatatcag gagttccctt tttcacagat actgtggcaa
     2101 ggtccccgat acccagtttc ggctgccggt tcttgacacc gtgataaccg aaaacggaaa
     2161 caatctgaac aacacgggca cccgtgttgt ctgcacaaac catcttggag ccggtctgaa
     2221 gtgcgcgcgg gattttggac gttaagcctc tcatgcccgg gtcacctcaa caacaacgta
     2281 ggaggttgtc ttgttcaatg gtctgcactc tgcaatcttg acctcgtcgc caattcttgc
     2341 gtcgaggcat ggggccatgt gtgcgtggat tctggaactg cgcttctcat atctgtcata
     2401 ttttctgaca aagtgaaggt agttccgctc gacaacaacg gtcccctgca tgcgctcact
     2461 cacgacctta ccggtaatca cctggccgcg taccggtaag ctgccgtgga atgggcagtt
     2521 tacgtcgtca cagtccttct ctggaactgc aacatttaag ccgatatttt ttgccatatt
     2581 taaccctcat cttatgcgca tagctcgcac gtcacgcacc cctaaacggg acataacgcc
     2641 tcgctgccct gggcttacgc cctcgctttt acacgccgcg ctggagaaat agacaagcct
     2701 gagcagtcta ttacaacttc aacactatcc gggagggtta cccggagtaa catatattgt
     2761 ttctccaagc acttaattcc attccctgat cggagggaaa gagtattttt ggtctcatcc
     2821 acgatgaaac cacagattcc ctcctgtgtt ttgttgcgtg accgaaccac aaccgcatat
     2881 aatccgatca gctcatgctg caggatcgat tgtggcgtga tcatagacac ttatgcctga
     2941 cgcttggtct gttcagtctt aatgcgtgcg atagttctgc ggatttcccg tatctttccg
     3001 gggttttccg gtgcaccacc ggcgctgact tttccgtact gctggattaa ctcatttctg
     3061 agtttctgct cgttctctac aagctcagca tcggaaaact gagcgacttc ctttgctctg
     3121 aagattgcca tgttactcag gcctcctcag ggaactcaga ttcaagttca ggttcgtttg
     3181 caagttctgc atcgaactca tcaaacacgt cagcctcggc aacctgtgcg ggagctgggt
     3241 ttgcatccgg gcggatctca aagtgatccg gaagaactgc tcctggcgga acaatcttta
     3301 cctggacacc gatgataccg agcttcttga ctgcggttgc atatcccgtc tcaacaacgg
     3361 actcagccgg ttcaccggag tgcttgatgt agccttccac aaatttctgc acacgggcac
     3421 gggcaccggt cagcttacct gcaatgataa cctcacagcc aagtgctcca gagtccatga
     3481 cacggcggat aacggaggtt cctgccttcc ggaagtacca tccgcgctca agtgcatttg
     3541 caaggcgctc tgccataatc tgtgcattca ggttcgggtt tgcaacctgc tgaacctcaa
     3601 cctgcgggga gtcgatacca tagacggagg tcagatcagt cgtaatctgg cgaaccagtc
     3661 tgccgccctt gccgataacg atacccggtt tttctgcaaa gatggtaacc tgcgtaccaa
     3721 ccggggtacg gacgatgtcc ataccgccgt atccggcgcg cttgagttct ttgttgaggt
     3781 actgctcgac acggaccttg cggacaccat cggcaacgaa cttcttctcg atagtcatac
     3841 ttactgcacc tcacgaagga ttaactcaat gttcacggta tctctgtgtt tgggagttgc
     3901 tctgcccatt gcacggggga agattgcctt catgcagcgt cctttgtttg ctgcggcgtg
     3961 gacaataacc atcttttccg gagcaagacc tgcatactca gcgtttttct gtgcggagcg
     4021 gattaaccgg atgtactctg cggctgcctt gaccggatat cttccggctg cggtgccgaa
     4081 ggtcttaccg tttaagctct tctggtgacc aacgtttctt gcatagcggt ggaacggtac
     4141 agctctcttc agagcaataa cctgttcaag gtacgcaaca gcgtcgtctg ccatcatgtt
     4201 gcggacgagg tgggcaatct ccactgcgtg cttcggagag catccaagct cgttagcctt
     4261 tgcacgggcg atgttgtcgc cggtaagttt gttactgtat tctgttcttg ccatgacaat
     4321 cacttcagcg ggacatactt actcgaccgg gttgcaccaa taccggcgct accgtggctg
     4381 accttcttgc gggtaagagc gaactcacca aagtag
//
LOCUS       DALTZZ010000245         2088 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_40293,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000245 DALTZZ010000000
VERSION     DALTZZ010000245.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40293"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="O0X12_04770"
     CDS             complement(<1..293)
                     /locus_tag="O0X12_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53499.1"
                     /translation="MVEHKPLKKSSPGTKTDAAVPGGMVARRGQFLQAMRQMTFEAGH
                     FTTAELAEAASVPRSTAQDWINRLLREGCIFTKEEKHGRSPARYASRSAMPSTT"
     gene            complement(280..1269)
                     /locus_tag="O0X12_04775"
     CDS             complement(280..1269)
                     /locus_tag="O0X12_04775"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HJK53500.1"
                     /translation="MKVALLGLGSVGRGVAQVLSNPSYGFVITAAADSKSGVVAEQGT
                     SLNMDDVFSRKAATGKCGDAAWTADRIVREAEYDILVEVTPTNAQTGEPALSNIRAAL
                     QRGKHVVTSNKGPVSIAYPELSRLAAEHNVAFLFEGTVAGAVPIINGLTHGLAGNKVH
                     QLFGILNGTCNYVLTRMEEEGLTYSQALDEARDLGYAEADPTYDVNGTDAAIKLVILA
                     NAVFNMGVTLDDVIRIGIDRLTPDALRMAGTTGHTIRLIASIHPDKRLLEVSPRLIPK
                     DHPLVVEGTLNSVTVETELAGPITFIGRGAGSVETASAVLADLLAVKERYGGA"
     gene            complement(1275..1793)
                     /locus_tag="O0X12_04780"
     CDS             complement(1275..1793)
                     /locus_tag="O0X12_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HJK53501.1"
                     /translation="MGLDTVQISLRLELNDKPGQLLAAIKPVSDSGANIISIMHQRGA
                     EASNGTLIVDIVVSLPQSRLDQLKTALTENGIEIIRIGTEHLTCTKTFILIGHILHTD
                     LTDTVNRIDKANIAEVTELHIIMPHITQPSTAKLTIKAAGAAEMEQAFEILMKIAEEK
                     HLLIVDEMEDEP"
BASE COUNT          524 a          546 c          500 g          518 t
ORIGIN      
        1 gtggtggacg gcatcgcact tcgtgatgcg taccgagcag ggcttctccc gtgcttctct
       61 tctttggtaa aaatgcaacc ttcccgcaga agccggttga tccagtcctg cgcggttgag
      121 cgggggacag aagcagcctc cgcaagttca gcagtggtga aatggcctgc ttcaaacgtc
      181 atctgacgca ttgcctgcag aaactgaccg cgccgcgcaa ccatccctcc gggaacggct
      241 gcatctgtct ttgtgcctgg cgaactcttt ttcagaggtt tatgctccac catagcgctc
      301 cttgactgca agcagatctg caagaactgc acttgccgtc tcaacagatc ccgctcctct
      361 tccaatgaac gtaatgggac cggcaagttc agtctccacc gtcaccgagt tcaaagtacc
      421 ctccactacc agcggatgat ccttggggat cagcctgggt gatacttcaa gcagtcgttt
      481 atctggatga atggatgcaa tcagccgaat ggtatggccg gtcgtacctg ccatgcgcaa
      541 tgcatcgggt gtgagcctgt caataccgat ccggatcacg tcatccagcg taacacccat
      601 attgaatacg gcatttgcca gaatcaccag tttgattgca gcatccgttc cattcacatc
      661 gtacgtgggg tcggcctcag cataaccgag atcgcgcgcc tcatcaagtg cctgggaata
      721 ggtaagaccc tcttcttcca ttcttgtaag aacgtaattg caggtaccgt ttaagatgcc
      781 gaacagctga tggactttgt ttcctgcaag accgtgtgtg agaccgttga taatgggaac
      841 tgcccctgca acggttccct caaaaagaaa tgccacattg tgttctgccg caagccggga
      901 aagctctggg taagcgatag agaccggccc tttatttgag gttacaacat gtttcccccg
      961 ttgtagtgca gcacggatat tggacagggc cggttcacca gtctgtgcat tggttggcgt
     1021 aacctcaaca agaatatcgt actcagcctc tctaacaatc cggtctgcgg tccatgcagc
     1081 atccccgcat ttgccagtcg cagctttgcg ggaaaacaca tcatccatat taagggatgt
     1141 accctgctca gcaacaacac cgctttttga gtcggctgca gcagttatca caaatccgta
     1201 ggatgggttt gaaagaacct gggcaacacc tctgccaact gacccaaggc caagaagagc
     1261 aactttcatg ggggtcatgg ctcatcctcc atctcatcaa caatcaagag atgtttttct
     1321 tcagcaatct tcatcagaat ctcaaacgcc tgctccatct cggcagcacc cgcagctttg
     1381 atcgtaagtt ttgctgtgga cggctgggtt atgtgcggca taatgatgtg aagctcggtc
     1441 acttctgcaa tatttgcctt atcgatacgg ttaacagtgt ccgtcagatc tgtatgcaga
     1501 atatgaccaa ttaaaataaa cgtcttcgta caggtcagat gctctgttcc gatacggatg
     1561 atctcaatgc cattttcagt gagcgctgtc ttcaactgat cgagacggct ttgagggagg
     1621 gagactacaa tgtccacgat cagggttccg ttgctcgctt ctgcaccgcg ctggtgcatg
     1681 atagagataa tattcgcacc gctgtcggaa accggcttaa ttgccgcaag gagctgaccc
     1741 ggtttgtcat tgagttcaag tcgtagtgat atctgcaccg tatctaatcc caataataat
     1801 ccgtcaccca tccattttaa tgtggggttt gtcagcggag atgaaccaca actccaaaat
     1861 cagatgctaa gtggacaact gttttcccag tgatataaga tggttagaag acagtatggt
     1921 tttggaaaaa ccagtatccc aaaataacct gcacgatact ggattacacc gctcaaaaat
     1981 atcagagaag aagagtagct gctgtcgtac agatccagta aacgaccatg gcaaacaaac
     2041 caccggaaag tgttgcaagc aggtttgttc ccgaattacc gataacac
//
LOCUS       DALTZZ010000246         2006 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_40408,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000246 DALTZZ010000000
VERSION     DALTZZ010000246.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40408"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..724)
                     /locus_tag="O0X12_04785"
     CDS             complement(<1..724)
                     /locus_tag="O0X12_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJK53502.1"
                     /translation="MTLEDWYRQDRLPHIYCAGCGNGTILNCTLRAVNELGLDRDKTT
                     FVSGIGCSSRAAGYTSCDSLHTTHGRALPFATGVKLVKPENNVIVFTGDGDLSAIGGN
                     HFIHACRRNIDLTVVCMNNSIYGMTGGQGSPCTPYGAITTTTPYGMHEMPFDLCAVAE
                     AAGANYVARWTSFHVKELTAAIRAGIETPGLSFIEVMAQCPTSFGSKNKMKQVSQMID
                     MFRTNAVLKGKADRDAAAGIPLA"
     gene            complement(721..1854)
                     /locus_tag="O0X12_04790"
     CDS             complement(721..1854)
                     /locus_tag="O0X12_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HJK53503.1"
                     /translation="MTDKIEFLNGNTACAEGAIAAGCRFFAGYPITPSTEVAERMAAR
                     LPKVGGTFIQMEDELASMAAIIGGAWAGARTMTATSGPGFSLMMENIGYAAMTETPCV
                     VVNVQRGGPSTGQPTMAAQGDMMQARFGSHGDYSIIALSPSSVQEMFDLTVKAFNLAD
                     RFRTPVFLMADETIGHMREKVVLRDNVEITPRRELSTGDLPCQPDVEGVPGFGTFGNG
                     HGVHITGLTHDERGYPATDSAALHESLVRRQVGKIENFRNEIADVDIVNPDAEIVFIS
                     YGAPTRAVQQLLADYPGVYGHLNLRIVWPFPDAKLSEFHAVKAFVVPEMNLGQIAREV
                     RMHTNVPVVTAPKLGGALHTVDELKHAADVAETATGTFTEVLL"
     gene            complement(1851..>2006)
                     /locus_tag="O0X12_04795"
     CDS             complement(1851..>2006)
                     /locus_tag="O0X12_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HJK53504.1"
                     /translation="VLDHPERCTNCRLQIMYGRQLCGMCQMTCPDQAISWVEEKPYEA
                     HKVVIEY"
BASE COUNT          469 a          523 c          547 g          467 t
ORIGIN      
        1 gagcaagcgg aatgccggca gccgcatcac gatcggcttt tcctttcaga accgcattcg
       61 tacggaacat gtcgatcatc tgagagacct gcttcatttt gtttttactg ccaaaggacg
      121 tggggcactg tgccatgacc tcaatgaagg agagaccggg tgtttcaatt cctgcacgga
      181 ttgctgcagt gagctccttg acgtggaacg aggtccagcg tgcaacatag tttgcaccgg
      241 ctgcttcggc aacagcacaa agatcaaacg gcatttcatg cataccgtag ggagtggtag
      301 tagtgattgc accgtacgga gtgcaggggc ttccctgacc tccggtcatc ccgtagatac
      361 tgttgttcat gcagacaacg gtgagatcaa tgtttctccg gcatgcatga atgaaatggt
      421 taccacctat tgcggaaaga tcaccgtccc cagtaaagac gatgacgtta ttttccggtt
      481 ttacaagttt aacaccggtt gcaaacggca gtgcacgacc gtgcgtagtg tgcagggagt
      541 cacaagaggt gtatccggca gcacgtgaag aacaaccaat tccagagaca aaagttgttt
      601 tgtcgcggtc cagcccaagt tcgtttactg cccggagtgt gcagttaagg atggttccgt
      661 tcccgcaacc tgcacagtag atgtgaggta ggcgatcttg gcggtaccag tcttcgaggg
      721 tcacagcagc acctccgtga atgtaccagt agcggtttcc gcaacgtctg cggcgtgctt
      781 aagctcgtca acggtgtgca gtgcaccacc gagtttgggg gctgtgacaa ccgggacgtt
      841 ggtatgcata cggacctcgc gagctatctg gccgaggttc atttcgggaa cgacaaatgc
      901 tttcactgca tggaactcgg aaagctttgc gtccgggaac ggccagacaa ttctaagatt
      961 taagtggccg tatactcccg gatagtcagc cagaagctgc tggactgctc tggttggtgc
     1021 tccatagctt atgaaaacaa tttctgcatc agggtttacg atgtcaacat ctgcgatctc
     1081 atttcggaag ttctcaattt tcccgacctg acgtctgaca aggctctcgt gcagggctgc
     1141 ggagtcggtt gccggatatc cccgctcgtc atgggtgagt ccggtgatgt gaacaccgtg
     1201 tccgtttccg aaggtaccga aacctggaac tccctccacg tccggctggc agggaagatc
     1261 acctgtagag agttcacgac gtggagtaat ctcgacgttg tcccgtaaaa caaccttttc
     1321 ccgcatgtga ccaattgttt catccgccat gagaaaaacg ggggtccgga atcggtctgc
     1381 aagattaaag gctttgacag tcagatcaaa catctcctgc acagaggatg gagaaagggc
     1441 aatgatgctg taatctccat gagagccgaa ccgggcctgc atcatatctc cctgagccgc
     1501 cattgtgggc tgacccgtgg atgggcctcc acgctgaaca ttgacaacca cgcagggagt
     1561 ctcggtcatg gcagcataac ctatgttttc catcatgaga gagaaaccag ggcctgaggt
     1621 tgcagtcatc gtacgtgcgc ctgcccaagc gcctccaatg attgcagcca tactggcaag
     1681 ctcatcctcc atctggatga aggtcccgcc gactttagga aggcgggctg ccattcgctc
     1741 agcaacctcc gtggacggag taatcgggta tccggcaaag aagcggcacc cagcagcaat
     1801 tgcaccctca gcacaggcgg tatttccatt gaggaattcg attttatcag tcaatattca
     1861 atcaccactt tgtgtgcctc gtagggtttt tcctctaccc atgagatggc ctgatccgga
     1921 caggtcatct gacacatccc gcagagctgg cgaccataca ttatctgcag gcggcagttg
     1981 gtgcagcgct caggatggtc aaggac
//
LOCUS       DALTZZ010000247         3918 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_40886,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000247 DALTZZ010000000
VERSION     DALTZZ010000247.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40886"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            30..656
                     /locus_tag="O0X12_04800"
     CDS             30..656
                     /locus_tag="O0X12_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HJK53505.1"
                     /translation="MAVMMRIKGFFSKLFGKKRSRVGIYGPPNAGKTTLANRIVRDVT
                     GEAIGPVSEVPHETRRARRKEGVEINSGGNKLLIDIVDTPGVTTKIDYHEFLEYGMDQ
                     DEAVARAREATEGVAEAMHWLREDIDGVIYVLDSTQDPFMHVNIMMVGIIESRKLPVI
                     IVANKIDLPDAAPQRIKSAFPQHPVVMVSGLEGNNMDDLYAKIAEVFR"
     gene            663..1037
                     /locus_tag="O0X12_04805"
     CDS             663..1037
                     /locus_tag="O0X12_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HJK53506.1"
                     /translation="MIQGVQIDMLSSDRLSRMTSMEKIRLILDDVRQGYIVVLEKGLT
                     PEEQGKLLETTMMEISPGGFSGIEIETYPSNNASEGGLFSKLFGGNKNPAGRLMVIGP
                     VDQLKMLTREKDRLIAWASAAQ"
     gene            1068..1448
                     /locus_tag="O0X12_04810"
     CDS             1068..1448
                     /locus_tag="O0X12_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing protein"
                     /protein_id="HJK53507.1"
                     /translation="MPHKCTQCGREFRDGSVEILRGCPSCGGKKFLYVSDRVRNADVL
                     EEKSIADIAQETKQEVLEVKNQGPASRPTDILERVESVRILSPGKYELNLERLAESQD
                     IIIGVDDGKYMLDLPSMSKKKQKK"
     gene            1670..3628
                     /gene="gyrB"
                     /locus_tag="O0X12_04815"
     CDS             1670..3628
                     /gene="gyrB"
                     /locus_tag="O0X12_04815"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HJK53508.1"
                     /translation="MTDNYDASHITVLEGLAPVRERPAMYIGSTDTRGLHHLVYEVVD
                     NSIDEALAGFCKHISITLNQDGSCTVEDDGRGIPVDIMPKQNKSALEVVMTVLHAGGK
                     FDKNTYQVSGGLHGVGVSVVNALSTRLTVEVFRDGNVYSMAFSRGGLVQKLECRPETE
                     NEFRERRTRLDNTEAKSPAELTGTRITFQPDGAIFDTTHFDYDILAHRFRELAYLNKG
                     VELRVADHRTGDSDVFCYEGGLREFVAHLNEGKELLHTDIIYLDKSDTDNKIDVEIAL
                     QYNQTYSETLFTFVNSVNTREGGTHLEGFRSALTRAINNSAHANKHLKEDVSVKGEDV
                     REGLTAVISVKIANPQFEGQTKMRLGNSNVKGLVDSMVYQALTEFFEENPKVIAAIAK
                     KAQEAANAREAARRAKELARRKSSLEMSGLPGKLADCSERDPAKSEIYIVEGDSAGGS
                     AKQGRDRKFQAILPLRGKILNVEKAIEHKALKNAEIQTLISAVGAGYGESFDPERARY
                     HHIVIMTDADVDGAHIRILLLTFFYRYMKPLVELGYVYVAQPPLFRIAKGKQERYVYT
                     EEDMRTVVAEFGEKGVVVQRYKGLGEMNAGQLWETTMNPENRILKQVRIEDASYANEI
                     FEKLMGDDVMPRKDFIKRHAGEVKNLDI"
     gene            3693..>3918
                     /locus_tag="O0X12_04820"
     CDS             3693..>3918
                     /locus_tag="O0X12_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53509.1"
                     /translation="MKNCFIDYAMSVIIGRAIPDVRDGLKPVHRRILYAMFHDENNTS
                     DKAYKKSAKAVAATMGNYHPHGDAAIYDTLV"
BASE COUNT         1032 a          890 c         1042 g          954 t
ORIGIN      
        1 ctactacgta tcccaattaa ggtgttttca tggcagtaat gatgcgtata aaaggctttt
       61 tctcaaagct gtttggtaaa aaacggtcgc gtgttggaat ttatggtccg ccgaatgccg
      121 gtaaaaccac acttgccaat cgcatcgtcc gtgatgttac gggagaagcg ataggtcctg
      181 tgagtgaggt cccgcatgaa acccgccgtg cccggagaaa ggagggtgtt gagatcaaca
      241 gcggcggtaa caagcttctg atcgatattg tggatactcc gggagttacc acaaagatcg
      301 actatcatga gttccttgag tatggtatgg atcaggatga ggcggttgcc cgtgcccgtg
      361 aggcaacgga aggtgtcgcc gaggcaatgc actggctccg tgaagacatt gacggtgtca
      421 tctacgtgct tgacagtact caggatccgt tcatgcatgt aaacatcatg atggtcggta
      481 tcattgaatc ccgcaaattg ccggtcatta ttgtcgctaa caagatcgat ctgccagacg
      541 ccgcaccgca gcggattaag agtgcattcc cgcagcatcc agttgtgatg gtctccggtc
      601 ttgaaggaaa caacatggac gatctgtatg caaagatcgc cgaggtattt aggtgaagaa
      661 aaatgattca gggcgttcag attgatatgc tgtcaagcga tcgcttgagc cggatgacat
      721 ccatggagaa gattcgcctt atccttgatg acgttcgtca gggttatatc gttgttcttg
      781 aaaagggcct gactcctgag gagcagggaa aacttctgga aacaacgatg atggagatat
      841 ctcccggcgg attttcggga attgagatcg agacataccc ttccaacaat gcttcagaag
      901 gtggtctgtt ctcaaaactg ttcggcggaa ataaaaatcc tgccggcaga ctgatggtga
      961 tcggaccggt cgatcagttg aagatgctta cccgcgagaa ggaccgtctg attgcctggg
     1021 cgtctgctgc acagtaaaca ccatttcgtt catcgaaaca tacagccatg ccacataaat
     1081 gtacccagtg cgggcgcgag ttccgcgatg gttcggtcga gatccttcgc ggatgtccga
     1141 gttgcggcgg aaaaaagttc ctttacgtca gtgaccgcgt gcgtaatgca gatgttcttg
     1201 aggaaaagag cattgccgat attgcacagg aaacaaaaca ggaggtgctt gaggttaaga
     1261 atcagggacc tgcatcacgg ccgacggaca ttctggagcg ggtggaaagt gttcgtattc
     1321 tgagtccggg gaagtatgaa ctgaatctcg aacggcttgc cgaatcgcag gatattatca
     1381 tcggtgtcga tgacggaaag tacatgcttg atctgccgtc gatgtcaaag aaaaaacaga
     1441 agaaataatc ctcctttttg gatctttttt tagaacgtat ttttccaccc tccgaatcct
     1501 gttcgttttt atctgccagc aatctaaata tgtcattcac actaatataa taaggaataa
     1561 atatttttca gagtgcgccc tattattctg cgcggttttg aaaaataagt taactatttc
     1621 gtagaaacac cctgcctggg tggttctttt caaaggagat cacatattaa tgaccgacaa
     1681 ttacgatgcg tctcacatca ccgttctcga gggtcttgct ccggtgcgtg agcgccctgc
     1741 catgtatatc ggcagtaccg atacccgtgg tctgcaccat cttgtctatg aggttgtgga
     1801 caactccatt gatgaagctc ttgccggatt ctgtaaacac atctctatca cactgaacca
     1861 ggacggatcc tgtacagttg aggatgatgg acgcggaatc ccggttgaca ttatgcctaa
     1921 acagaacaag agcgctcttg aagttgtgat gacggttctg catgccggag gtaaattcga
     1981 taaaaacacc tatcaggttt ccggcggtct gcacggggtt ggtgtctctg tagtaaacgc
     2041 cttgtccacc cgcctgaccg ttgaggtgtt ccgggatggg aatgtctact caatggcttt
     2101 ctcccgcggt ggtcttgttc agaaactgga atgccgtccc gagacggaaa atgagttccg
     2161 tgagcggcgc acacgcctgg acaacaccga ggcaaagagt cctgcagaac ttacgggtac
     2221 tcgtatcacc tttcagccgg acggagcaat ctttgataca acccactttg actatgatat
     2281 ccttgcccac cggttccgtg agcttgctta tctgaacaag ggtgtggaac tgcgtgttgc
     2341 ggatcaccga actggcgatt cggatgtgtt ctgctatgaa gggggtcttc gtgagtttgt
     2401 tgcccatttg aatgagggca aagagctgct gcataccgac atcatctacc ttgacaaatc
     2461 agacaccgat aacaagatcg atgttgagat tgcacttcag tacaatcaga cctacagtga
     2521 gacactgttt acatttgtga acagtgtcaa tacccgtgag ggagggaccc atctcgaagg
     2581 tttccggtca gcactcaccc gtgccatcaa caacagtgct catgccaaca aacacctaaa
     2641 agaggatgtc tcggtcaaag gcgaggatgt ccgtgaaggc ttaacagctg tcatcagcgt
     2701 caagattgca aatccccagt ttgagggtca gacaaagatg cggcttggca acagcaatgt
     2761 caaaggtctt gtggactcta tggtctatca ggcactgact gagttctttg aggaaaatcc
     2821 caaagtgatc gcggcgattg caaagaaagc acaggaagct gcaaacgccc gcgaagctgc
     2881 acgccgtgca aaagaacttg cacggagaaa aagttccctt gagatgtcgg gtcttcccgg
     2941 taaacttgct gactgttccg agcgtgatcc tgcaaagagt gagatctaca tcgtggaagg
     3001 agattcggca ggaggttccg caaagcaggg acgtgaccgg aagttccagg ccattcttcc
     3061 ccttcgtgga aaaatcctga acgtggaaaa agccatcgaa cacaaggcgc tcaaaaatgc
     3121 ggagattcaa actctgatat ctgcggttgg tgccggatat ggtgagtcat ttgatccgga
     3181 acgtgcccgt taccaccata tcgttatcat gacggatgcg gatgtggacg gtgcacacat
     3241 caggattctc ctgcttacct tcttctaccg gtacatgaaa ccgcttgtag aacttggcta
     3301 tgtgtatgtt gcccagccgc cgctgttccg gattgcgaaa ggaaaacagg aacgttatgt
     3361 ctataccgag gaggacatgc gtacggtggt cgctgagttc ggtgagaaag gtgttgttgt
     3421 acagcggtac aaaggtcttg gtgaaatgaa cgcaggccag ctttgggaaa ccacgatgaa
     3481 cccggagaac cgtatcttaa aacaggtccg aatcgaggat gcaagttatg caaatgagat
     3541 ctttgaaaaa ctcatgggtg atgatgtgat gccgagaaag gatttcatta agcgtcatgc
     3601 aggggaggtg aagaaccttg acatctgagg agcaggcacc gctcgtagaa aagattacgc
     3661 accgtaccga accggtgaac atcgaagatg agatgaagaa ctgctttatc gactatgcaa
     3721 tgtcggtaat tatcggtcgt gcaattcctg atgtgcgtga tggtctgaaa cctgttcacc
     3781 gccgtattct gtatgcgatg ttccatgatg agaacaacac gagcgacaag gcctacaaga
     3841 aatcagcaaa ggcagtggct gccacgatgg gtaactacca tccgcacggt gatgcggcta
     3901 tctacgacac ccttgtta
//
LOCUS       DALTZZ010000248         2326 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_41630,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000248 DALTZZ010000000
VERSION     DALTZZ010000248.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41630"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="O0X12_04825"
     CDS             complement(<1..396)
                     /locus_tag="O0X12_04825"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJK53510.1"
                     /translation="MVKKPCVQNPSDPVLWCKEAAARIEAGDHKGAIYCYEESLKIRP
                     EVPDVWYNLGLLLEKIGQPTAALGCYTKSSEMFPGDYRFFAERARLLASAGKYLDAVV
                     AVSDALAINPHEPQLHANKAGYLIRAGDAK"
     gene            complement(396..1679)
                     /locus_tag="O0X12_04830"
     CDS             complement(396..1679)
                     /locus_tag="O0X12_04830"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HJK53511.1"
                     /translation="MVRPVLQVALDLTELSRAEKIAEEAVAGGADWIEIGTPLVKSEG
                     MESVRRMRDLFPTTTLVADLKTSDTGAIEVEMAAKAGANVVCVLAAADDAVIADALRG
                     AALYGVEIMADMMNVSDPAARAEELAALGVQIINAHVGIDQQMTGKDPLALLDRLGKL
                     PVKIAVAGGLNAETAAAAAARGADIVIVGGTIIKAADVTAAAAAVRAALDSPKAAQEE
                     KKTLDDQIRELLQTVSAPNVTDALYRKGAMAGLIECHVPHKMIGRAVTVQTFGGDWSK
                     PVQAIDSCMPGDILVISNDKRTDVAPWGELATHSAMNRGVAGIVIDGAVRDWDDIQTL
                     DIPVFATAVQPNAGEPKGFGEIGAEISCCGQTVRPGDWLIGDQSGVVVIPRERAYEVA
                     RRAVEVWKTETRVREEIRRGGTLGSVTQLLRWEKK"
     gene            complement(1788..2318)
                     /locus_tag="O0X12_04835"
     CDS             complement(1788..2318)
                     /locus_tag="O0X12_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HJK53512.1"
                     /translation="MNVRNPGIANFGITVIQSRHSVRKFKDEEIPPEFIRKALECASK
                     APTGRNKQPWIFVVIKNKETLAKIAELAPNGKFIEGAAVGILVLGEADWKFTVEDCSA
                     ATENLLLALHAYGYGGCWIAGNQMPYADAVRELVHVPETYKLVSIIAAGVPDVGGITL
                     AEKIPFEKLIYEEKYQ"
BASE COUNT          476 a          703 c          581 g          566 t
ORIGIN      
        1 ctttgcatct ccggcacgga tcaggtagcc agccttgttt gcatggagct ggggctcatg
       61 gggattgatt gccagcgcat cgcttacggc aactactgca tcaagatact ttccggcaga
      121 tgcaagcagt cgtgcacgct ccgcaaaaaa ccggtagtct cccggaaaca tctccgatga
      181 ctttgtgtag cagccaagtg ccgctgttgg ctgaccgatc ttttccaaaa gtaacccaag
      241 gttgtaccag acatccggca cctccggccg gatcttcagc gactcctcgt agcagtagat
      301 ggcacctttg tggtcccctg cctcgattcg tgcagcagcc tctttacacc agagcaccgg
      361 atcggaagga ttctgaacac acggtttttt taccattatt ttttctccca gcgaagcagt
      421 tgcgtcacac ttcccagcgt tccgccgcgg cggatctctt cccttacccg cgtctctgtc
      481 ttccacacct ctaccgcacg gcgggcaacc tcgtaggcac gttcacgggg aattaccaca
      541 acacctgact gatcgccaat cagccagtca ccgggacgaa ccgtctgacc gcagcaggaa
      601 atttctgcgc cgatctctcc gaaccctttc ggctcgcccg cgttcggctg caccgcggtt
      661 gcaaatacgg ggatgtccag tgtctggata tcatcccagt ccctgactgc tccgtcaatg
      721 acaattcctg caaccccccg gttcatcgca gaatgcgtgg cgagttctcc ccacggtgca
      781 acatcagtac gtttgtcatt actgatgacc agaatgtccc ctggcataca ggagtcaatc
      841 gcctgaaccg gcttggacca gtccccgccg aaggtctgca cggtcacggc acgaccgatc
      901 atcttgtgcg gaacatggca ctcaataagt cctgccattg ctcctttgcg gtaaagggcg
      961 tcggtgacgt ttggcgcgga tacggtctga agcagttcgc gtatctgatc gtcaagagtt
     1021 ttcttctcct cctgtgccgc cttaggactg tcgagtgctg cacggacggc tgcggctgcg
     1081 gcggtcacgt ccgctgcttt gatgatcgtt cctccgacga tgacaatatc cgcaccgcgt
     1141 gctgcggctg ccgcagcggt ttctgcattg aggcctccgg caaccgcgat cttcaccggc
     1201 agttttccaa gcctgtccag cagtgcaagc ggatcttttc cggtcatctg ctgatcgata
     1261 ccgacatggg cgttgatgat ctgtacccca agtgcagcca gctcctcggc ccgggcagcc
     1321 gggtcactga cgttcatcat atcggccatt atctcaacac catacagtgc cgcgcctctc
     1381 agtgcatcgg caataaccgc atcatccgct gcggcaagca cacagacaac gttcgctccg
     1441 gcctttgctg ccatctccac ttcaatcgct ccggtatcgg aggtcttgag atcggctacc
     1501 agcgttgtgg tcggaaagag atcccgcatc cggcggacac tttccatacc ttcgctcttc
     1561 accagaggag ttcctatctc tatccagtcg gcaccgccgg caaccgcctc ctcggcgatc
     1621 ttctctgccc gggagagttc cgtaaggtcc agtgcgacct gcaaaaccgg tctgaccata
     1681 tcaactaatt tggtgaagcg aggggataag gttggtgatg tactggatga taccgtattt
     1741 ggcagaaaaa aaaggattgt tcggtcacgt gaaacgtgac tctgatctta ctgatatttt
     1801 tcttcgtaaa tcagcttctc aaacggaatc ttctccgcaa gtgtgatgcc gccgacatcg
     1861 ggtacccctg ctgcaataat ggagacgagt ttgtaggtct cagggacatg taccagctca
     1921 cgaaccgcgt ccgcgtaggg catctggttt cctgcaatcc agcatccgcc gtatccgtag
     1981 gcgtgcaggg caagaagcag attttcggtt gccgcagaac agtcctcaac ggtgaacttc
     2041 cagtcagctt ctccgagaac aaggattccc accgctgcac cctcaatgaa ctttccgttc
     2101 ggtgcaagct ctgcaatctt tgcaagagtc tccttgtttt tgatgacaac aaagatccat
     2161 ggctgcttgt ttcttccggt tggtgctttg gatgcacact ccagcgcctt tctgataaac
     2221 tccggcggga tctcctcatc cttaaacttg cggacactgt ggcgtgactg aattacggtg
     2281 ataccgaagt ttgcaatccc gggatttctt acattcatat gtactc
//
LOCUS       DALTZZ010000249         5018 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_41876,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000249 DALTZZ010000000
VERSION     DALTZZ010000249.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41876"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /locus_tag="O0X12_04840"
     CDS             <1..1276
                     /locus_tag="O0X12_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJK53513.1"
                     /translation="SKSDVALGDPSPSPRKGEFVHDPKQYVVIPAHTRPLHAVLTKKQ
                     ADVAKYLVEAGFNSADIRGTRAVIAGGISVSYVEEILPEDVSLIRIGAYPIDQTWLAD
                     VVAKHTEVLVVEELMPVIEEAVRQAACTTVVHGKLDGYLPHEGEFSTALVAKALVKSG
                     FLSENPFPPEPAQEAVAVRPPILCPGCLHRSVFYAMKKVFRDGVFPSDIGCYTLGLQM
                     GAVDTTICMGASVTVGSGISHACPGTDVVSTIGDSTFLHTGVNGLINAVYNNANQTII
                     ILDNRITAMTGHQPNPNSGVTATGVESPKISLEELARACGVSFVESVDPYDLTDLLET
                     LKAAKERPGVKVIIAKQPCVIMNKRNGVKRSRYVVDADACIKCGACIRYGCPALETRE
                     DGTSVTTALCTGCGVCADICPAGAIHRGGAKQ"
     gene            1273..1857
                     /locus_tag="O0X12_04845"
     CDS             1273..1857
                     /locus_tag="O0X12_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HJK53514.1"
                     /translation="MKKSYDVLIVGIGGQGTILASNVLGDACVIEGRHVRGAETHGMS
                     QRGGSVETHIRIDGKFGSLIAPGSADLLIAFDVLEALRYRHFLKENGVMVVNREMVVP
                     TSVFSAKLPVPQMEDVVAQLKSGKATVLLVDATEIATKAGSPLAANIVLLGAASHQLP
                     LSVSSLEEAVRRNVPQKTIALNEQAFAMGREVRL"
     gene            2060..2854
                     /locus_tag="O0X12_04850"
     CDS             2060..2854
                     /locus_tag="O0X12_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJK53515.1"
                     /translation="MTKNASATIQTLTILPGTTRAGEPENFSEITIRPGDTISIVGPT
                     GSGKTAFINDIEVFAQGDTITKRKILVNGAEPAEDLVRDPSKKPIAMITQNTKCLADL
                     TVEEFLAMHLRSRKMENPTIIAEAIDLANQFTGEKIAGCMRMSSLSGGQTRSLFVADA
                     VSISDTPIILLDEVENAGIFKDRVIEILREKHKAIIFVTHDPYVALLTEKRIVMKGGA
                     VEKILTPGDEERAALTQIAKMDADMMKLRERIRSGELLTASRGAEA"
     gene            2851..3558
                     /locus_tag="O0X12_04855"
     CDS             2851..3558
                     /locus_tag="O0X12_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HJK53516.1"
                     /translation="MNVRLIAIAGPPSAGKTAVVKQIIRNLGAAKVAYLKIDVVQAWE
                     DEELAAEFGIPTKKVYSGDMCPDHMGIMVIRDALLWADSLGAEYLLYESAGLCLRCTP
                     YTTHSLGICVLSAVSGTHAPLKMAPMIALADVAVVTKIDLVSQAEKEVFREGIRQVAP
                     EIDIVETNAIQGTGMRYLMQVIEQMKPAESEKDTLRGVPPLGVCTVCIGKKETGWQEH
                     FGVIRPLAAPDNLYRGE"
     gene            3559..4200
                     /locus_tag="O0X12_04860"
     CDS             3559..4200
                     /locus_tag="O0X12_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster protein"
                     /protein_id="HJK53517.1"
                     /translation="MVWTPPGKNCGACGMDSCDAFAAAVRESIKEVDECPYYLPQEPP
                     AVTQPCGSCALDAAYTGADVTNAPYDFIITAFPGEPSARKIVLPFRPDLVERLGITKG
                     DIVTGRPTGAGCPVQHVIKVLEADPMSGLITGHVVGPAFARGNDNVIDLRHYHMIGFE
                     GVANVVRRTPEFGLRMPFLPGVCMMHRTHTGVVNMIMQKSYGLHVRVEGVVIL"
     gene            4197..>5018
                     /locus_tag="O0X12_04865"
     CDS             4197..>5018
                     /locus_tag="O0X12_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 16 metalloprotein"
                     /protein_id="HJK53518.1"
                     /translation="MKSIEDINTKISEKTAVVWTASEFKKHIRAGETVTPEDVDVVTC
                     GTFGVMSGTAAVLAFQAAEPGTFTHAYKLTLNGVPAHIGPCPNESNGHVDAMVYGTAQ
                     SDRPGYGGGHLFADLVAGECVEAVIETDTGTITRSVTLGDMSSAKMIITRGTFKNYMA
                     FVNPSQEEITTIFAVLPMKGNLQEATFSGCGEINPLENDPELRYHTPGTAALVNGAPG
                     MILGTGTRTSAAKPNLSLAADMHQMDPNLMGGFKMNASCGCLTSVATAVPVTDAAT"
BASE COUNT         1225 a         1271 c         1366 g         1156 t
ORIGIN      
        1 ctcgaagagt gatgtggcgc tgggtgatcc gtcgccatcc ccaagaaagg gcgagtttgt
       61 tcatgacccg aaacagtacg tagtcatccc tgcccatacc cgtcccctcc atgcagtgct
      121 tactaaaaag caagccgatg ttgcgaagta tctggttgag gccgggttta actccgcaga
      181 tatccgggga acacgtgcgg tgatcgccgg aggaatctca gtgtcctatg tggaagagat
      241 tcttccggag gatgtatcac tcattcgtat cggtgcatat ccgatcgatc agacctggct
      301 tgcagatgtc gtggcaaagc acacggaggt tctggtggtg gaagagctga tgccggtgat
      361 cgaagaggcc gtacgtcagg cggcatgcac aaccgtagtg catggaaaac ttgacggtta
      421 cctgccgcat gagggtgagt tctccacggc ccttgttgca aaggcactgg tgaagtccgg
      481 tttcctttct gaaaatccgt tcccacccga accagcacag gaggcagttg cagtgcggcc
      541 gccgattctc tgcccgggat gcttgcaccg ttccgtgttt tatgcaatga aaaaggtgtt
      601 ccgtgacggc gtattcccca gtgacatcgg ctgttacacc cttggtctgc agatgggggc
      661 ggttgacacc acgatctgta tgggagcatc ggttacggta ggttcgggta tttcccatgc
      721 ctgtccggga acggacgttg tttcaacaat cggtgactcg acgtttttac ataccggggt
      781 aaacggattg atcaacgcag tgtacaacaa tgcgaaccag acgatcatta ttctggacaa
      841 ccgtattaca gcaatgacgg gccaccagcc aaacccgaac tctggtgtaa cagcaacagg
      901 tgtggagtct ccgaagatat ctctggaaga gcttgcacgt gcctgcggtg tttcgtttgt
      961 ggagtctgtt gacccgtacg atctgacgga tcttctggaa acactaaagg ctgcaaaaga
     1021 gagaccgggt gtgaaggtca tcatcgcaaa acagccgtgt gtgatcatga acaaacgcaa
     1081 cggtgtaaag cgcagccggt atgtagtgga tgcagacgcc tgcattaagt gcggagcgtg
     1141 tatccggtac ggctgccctg ctctggaaac ccgtgaggac ggaacatcgg taacaactgc
     1201 gttgtgtacc ggatgcggtg tgtgcgcaga catttgtcct gcgggtgcga ttcaccgggg
     1261 aggtgcaaag caatgaagaa gagttacgat gttctgattg ttggtattgg cggtcagggt
     1321 acgattctcg catcgaatgt actgggtgat gcgtgtgtga tcgaaggacg gcatgtccgc
     1381 ggtgcagaga cgcacgggat gtcacagcgc ggcggatcgg tggagacgca tatccggatt
     1441 gatggaaagt tcggttcact gattgcaccg ggttcggcgg atctgctgat cgcatttgat
     1501 gttcttgagg cactccggta ccggcatttc ctcaaagaga acggcgtgat ggtggtgaac
     1561 cgtgagatgg ttgtacctac ctctgtgttc tcggcaaaac tgccggttcc gcaaatggag
     1621 gatgtcgtgg cccagctgaa gtccggcaag gcaacggtgc tcttggtgga tgcaactgag
     1681 attgcaacaa aggcaggcag ccctcttgca gcaaatattg tcctcctagg tgctgcatcc
     1741 caccagcttc cgctctcggt ctcctccctt gaagaagcgg tccgcaggaa tgttccgcag
     1801 aaaacaattg ccctgaatga acaggcattt gcaatgggcc gagaggtccg tctctaacct
     1861 tttttttgat ttttgttttt gggaaacctt cgtggtacac taacgtacag attgtcattt
     1921 tggaacattg caaaatatcc aatacgccat attaattata ctaaacataa tctaaaatcg
     1981 attttacact tcttatagta ttaataatgt ataggatttc agaaaacggt tatatctcct
     2041 cacaccctat taagaagaga tgacaaagaa cgcttcagcc accatacaga cccttacaat
     2101 acttcccggt accacccgtg cgggagagcc cgagaacttt tccgagatca ccatccgtcc
     2161 gggggatact atctccattg tcggtccgac cggttctgga aaaaccgcat tcatcaacga
     2221 cattgaggtc tttgcacagg gtgacaccat caccaaacgg aagatccttg tcaacggtgc
     2281 agaacccgct gaagaccttg tccgcgatcc ctccaaaaaa cccattgcca tgatcaccca
     2341 gaacaccaaa tgccttgccg atctcactgt cgaggagttt cttgcaatgc atctgcgttc
     2401 ccgtaaaatg gagaacccca caatcatcgc ggaagcaatt gatctggcca atcagttcac
     2461 cggcgaaaag attgccggat gcatgcgaat gagttctctc tccggcggtc agaccagaag
     2521 cctctttgtt gctgatgcgg tcagtatctc cgacacaccg ataattctgc ttgacgaagt
     2581 ggaaaatgcc ggtatcttca aagatcgtgt cattgagatc ctgcgggaaa aacacaaagc
     2641 gatcatcttt gtcacccacg acccttacgt tgcccttctg acagaaaaac gaattgtcat
     2701 gaaaggcggt gccgtggaaa aaattctcac gccgggtgat gaagagcggg cagctctcac
     2761 ccagattgca aagatggatg ccgatatgat gaaactgcgg gaacgcatcc gttccggtga
     2821 actccttact gcatcacggg gagccgaagc atgaacgtcc ggctcatcgc aattgcagga
     2881 cccccgagtg ccggaaaaac tgcggtggtc aaacaaatta tcaggaatct cggtgccgca
     2941 aaggttgcat atctcaaaat tgatgttgtg caggcatggg aggatgaaga acttgccgca
     3001 gagtttggga ttccaacaaa aaaggtctac tccggcgata tgtgcccgga tcacatgggt
     3061 atcatggtga ttcgtgatgc cctgctctgg gctgacagcc tcggtgccga gtaccttctc
     3121 tatgaaagtg ccgggctctg tctgcggtgt actccctaca ccacgcactc tcttggaatc
     3181 tgtgttctct ctgcggtctc cggaacgcat gcgccattaa agatggcacc aatgatagca
     3241 ctcgcggacg ttgccgtcgt caccaagatt gatcttgttt cccaggcgga aaaggaagtc
     3301 ttccgagaag gaatccgcca ggttgctcct gagatcgaca tcgtcgagac caacgccatt
     3361 caggggaccg gaatgcgcta tctcatgcag gttatagaac agatgaagcc tgcggaaagc
     3421 gaaaaagaca cactccgcgg ggttccgcct ctcggtgttt gtactgtctg catcggtaaa
     3481 aaggaaaccg gctggcagga acactttggt gttatccggc cgcttgcagc acccgacaat
     3541 ctgtaccggg gtgagtagat ggtctggacg cctcccggca aaaactgcgg agcctgtggt
     3601 atggacagct gtgacgcgtt tgccgcagct gtacgtgaga gcatcaaaga ggtggatgaa
     3661 tgtccgtatt atctcccgca ggaaccgcca gcagtaaccc agccctgcgg ttcgtgtgct
     3721 cttgatgccg catataccgg tgccgatgtc accaatgccc cgtatgattt tatcattacc
     3781 gcattccccg gagaaccctc cgcacgaaag attgtcctgc cgttccggcc ggatctcgtt
     3841 gaaagacttg gcattacaaa aggcgatatc gttaccggca gaccaaccgg agcaggttgt
     3901 ccggtccagc atgtgatcaa ggttcttgag gctgatccga tgtccggcct tatcaccggc
     3961 cacgttgtcg ggcctgcatt tgcacggggg aacgacaacg tcattgatct caggcactat
     4021 cacatgatag ggtttgaggg agtagcaaat gttgtgcggc gaacaccgga gtttggtctc
     4081 aggatgccgt ttcttcccgg tgtctgtatg atgcaccgga cacacaccgg cgttgtcaac
     4141 atgatcatgc agaaatccta tggtctccat gtacgagtag agggagtggt gatattgtga
     4201 aatcaattga ggatatcaat acaaaaattt ccgagaagac tgctgttgtc tggacagcgt
     4261 cagagttcaa gaaacacatc cgtgcgggag aaactgtaac tcccgaggac gttgatgttg
     4321 tcacctgcgg aacgtttggg gttatgtccg gtactgcggc ggttcttgca tttcaggcag
     4381 cagagccagg tacatttacc catgcgtaca aactcacgct gaacggagtc ccggcacaca
     4441 tcggcccgtg cccgaatgaa agcaatggtc atgtggacgc aatggtgtat ggaacagcac
     4501 agtcagaccg gcccggttac gggggaggac acctgtttgc agatctcgtt gcaggagaat
     4561 gtgtggaagc agtgatcgaa accgataccg ggacaatcac cagatccgtg acacttggag
     4621 atatgtcctc ggcgaagatg atcatcaccc gcggtacatt caaaaattac atggcctttg
     4681 tcaatccctc acaagaggag atcactacaa tctttgctgt tctgccgatg aaagggaacc
     4741 tgcaggaggc aacattctcc ggatgcggcg agatcaatcc gctggaaaat gatccggaac
     4801 tccggtatca cacacccggc actgcggctc ttgtcaacgg agcacccggt atgatcctcg
     4861 gaaccggtac acggacatct gcggcaaaac ccaatctatc ccttgctgcc gacatgcatc
     4921 agatggatcc aaatctcatg ggcggattca agatgaatgc cagctgtggg tgtcttacct
     4981 cggttgcaac ggcagttccc gtaacagatg ccgcgaca
//
LOCUS       DALTZZ010000250         5778 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_42648,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000250 DALTZZ010000000
VERSION     DALTZZ010000250.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42648"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            24..145
                     /gene="rrf"
                     /locus_tag="O0X12_04870"
     rRNA            24..145
                     /gene="rrf"
                     /locus_tag="O0X12_04870"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(328..3108)
                     /gene="leuS"
                     /locus_tag="O0X12_04875"
     CDS             complement(328..3108)
                     /gene="leuS"
                     /locus_tag="O0X12_04875"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HJK53519.1"
                     /translation="MSEIEQEIRNTWIPAFESNPAEDKEKFYINVAFPYPSGAMHVGH
                     GRTYIVPDVVARFWRMQGKQVLFPMAFHVTGAPVLGIAKRIAKKDEKTLKLYGGLYRV
                     PQETLDSFTDPIVIVNYFADEYERVMKQLGLSIDWRRRFTTIIPQYSKFIEWQYSHIH
                     GQGKVVKGEYPVRYCPNCDQPVGDHDLLEGDSAEIMHFVFVKYPFGEFFIPTATLRPE
                     TIFGVTNLWANPEVTYLKAEVDGEKWIISREAAEKLRMQKHTVTEIGTISGAELVDKA
                     ATHPISGAAVPILPASFVDPDMGSGLVMSVPAHAPFDYIALRDLQQQGKYTEIVPIPL
                     VTVPNFGKIPAQEIVEKNKIPHQDDPRMDELTQELYTAEFSKGKLNENCGEHAGKSVR
                     QAREDVTREFVDTRGSIIFHDMSEKRVICRCGNRVYVKILDDQWFLNYADSAWKEQIH
                     AQLPKVALVPPEVRAEFERTVDWLKEWPCTRRVGLGTHVPWDPKWLFEPLSDSTIYMS
                     YYTIAHKITKIDAEKLTPAVFDYIFLGIGDCNALPVDAETLKELRSEFLYWYPYDYRF
                     SAKDLISNHLTFQLFHHRAIFPDDLQPKGMVVFGMGLLNGMKMSSSKGNVFLLEDAAN
                     EFGADTVRMFLVGSAEPWQDFDWRNELVLSVKKQIERMWQIVLDAAPATGSAPIDAWL
                     LSRLQHRIEATTKALLAFQTRQALQEAYNGIVSDLAWYRRRLPEGMTGTAVLQEVMSA
                     WIRLMAPVIPYTAEKLWSETGHEGLVSFASWPVADPALINESVEVSEELLQRTVEDIQ
                     SILKLVQIEATKVTLFVAPAWKHQVFKIVASAEDKRNVVKTVMADADLRAKGKDATDA
                     AMQTVKLIHSLPPELVTSIAAGVDELAIFTAAKAFLEKESGLAVEVVAAEASAHPKGK
                     LALPFKPAIVIE"
     gene            complement(3154..5187)
                     /locus_tag="O0X12_04880"
     CDS             complement(3154..5187)
                     /locus_tag="O0X12_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HJK53520.1"
                     /translation="MILVPDTSVVIDGRVTALIEQGEYRGATIIIPEAVFAELEAQAN
                     QGREIGFSGLSELQKLCKMKEEGLINLEYVGVRPKLEQVKLAGGGEIDSMIREVAIAH
                     NATFLTSDYVQAEVARAKGINVVFLRPEITDNFAPLLIDAFFDENTFAVYLKERVPPY
                     ARKGTIRESKLVTIRDTPATEYELRAIAQECLERAKRHPDGFVEVEMPGVTVSQIGSM
                     RITTTRPPFSDGIEITIVRPTREVSFDSYNYAAALKERLNIGNGGMLIVGTPGSGKST
                     LEQNIATYLSSENYIVKTIESPRDLMVPDKITQYTALDGSIAATGEVLTLLRPDYVVF
                     DEMRRSEELAVFADLRLSGIKVIGGLHARSAPDALIRLASLVDLNLIPQIVATVVFVK
                     DGDISDLYNVSTVFGVPAALERIGDPQVRPIVRFTNIITGVVAAEAFRYEGEVTVTSG
                     GGIHPAEQTETPAATVQVINATPAEVIETTATSVTESTSEVNGDGDDASHVPPPIHSM
                     PTYAVTEDNPAWIILEKEIQKEISRFTEGEIIVRMINDTKAAVYIEDADVPAAIGKAG
                     KNIAAVVSKVGVGIDVRPLSDLPGREVSTPAVPAGSGSSGAFKIRSEKKQLALICPEY
                     SNKIVDLFSGKEYLFTATVDVRGEIHLARNSSIAMEMIRRYGAGEELKIRPVD"
     gene            complement(5232..5609)
                     /gene="hisI"
                     /locus_tag="O0X12_04885"
     CDS             complement(5232..5609)
                     /gene="hisI"
                     /locus_tag="O0X12_04885"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HJK53521.1"
                     /translation="MTDLLDNLTYEGGLIPVIVQDIATKTVLMFAYANKEAVTLTKET
                     GFAHYYSRSRQKLWKKGEESGHLQKVCEIRVDCDHDCLLYLVEQTGCACHEGYFSCFF
                     RTIDGRIILPRLKDPQEIYHKIQ"
BASE COUNT         1355 a         1461 c         1360 g         1602 t
ORIGIN      
        1 aatcgggaac ttggcttggg ggattctgcg gccatagcag agaggttcca cccggaccca
       61 ttccgaaccc ggcagttaag ctctcttacg tcggatattg tactgagata cgcgagttct
      121 cgggaactat tcaacgctgc aaccccccct tcttcttatt cttgattcga ttaattgagt
      181 gatttctttt cttcggttga ggtaatcgtt ttctacattc aatgagtttt cattcttaac
      241 tggatctcta tcagagtctg tattctcttt ggctaacacc atcctgtagt taatggtgtt
      301 ttctgggaat tattaaaaaa aagaatatta ttcgatcaca attgcaggct taaatggcaa
      361 tgccagtttt ccttttggat gtgcacttgc ttcagctgca acaacctcta ctgcaagacc
      421 tgattccttt tccaggaatg ctttcgctgc agtgaaaatt gccagttcgt ccacaccagc
      481 cgcaatggat gtaaccagct ccggcgggag gctgtgaatc agttttactg tctgcatagc
      541 agcatccgtt gcatccttgc cctttgcgcg aagatctgca tcggccatta ctgttttcac
      601 cacattccgc ttatcttctg ctgatgccac aatcttgaac acctgatgtt tccaggcagg
      661 ggctacaaac agagtcacct ttgtcgcttc aatctgaacc agctttaaga tagactgaat
      721 atcctcaacc gtccgctgca gcaattcctc agaaacctcc acagactcgt ttatcagtgc
      781 gggatccgca accggccagg atgcaaatga tacaaggcct tcatgtcccg tttcggacca
      841 gagtttctca gcggtgtacg gaataaccgg ggccatcaac cgaatccatg ctgacataac
      901 ctcctgaagg acagcagtac cagtcatacc ctcaggtaac cggcgccggt accacgcaag
      961 atctgacaca ataccattgt atgcctcctg caatgcctga cgggtctgga atgctaatag
     1021 tgccttcgtt gttgcctcaa ttctgtgctg cagacgagaa agtaaccatg catcgattgg
     1081 tgctgaccca gttgccggtg cagcatccag tacaatctgc cacatccgtt cgatctgttt
     1141 tttcacagac agaaccagtt catttctcca gtcaaagtcc tgccagggtt ctgcacttcc
     1201 caccaggaac atacgcacgg tatctgcacc aaactcattt gccgcatcct ccagcaggaa
     1261 cacatttcct ttcgatgagg acatcttcat accattcagc aggcccatac cgaacacaac
     1321 cattcctttt ggctggagat cgtccgggaa gattgcacgg tggtggaaca actggaaggt
     1381 caagtgattt gagatcagat cctttgcact gaaccggtaa tcgtatggat accagtacag
     1441 gaactctgac cgaagttctt tcagtgtctc cgcatctacc ggtaaagcat tgcagtcccc
     1501 aattccaagg aagatgtaat cgaacactgc gggagtcagt ttctctgcat caatcttcgt
     1561 gattttgtga gcgatcgtat agtacgacat gtagatggtc gaatccgaaa gcggctcaaa
     1621 cagccacttt ggatcccatg gtacatgcgt tcccagacca acacgacggg tacatggcca
     1681 ctctttcagc caatcaacgg tgcgttcaaa ctctgctctg acctctggtg gtaccagtgc
     1741 cacttttggc agctgagcgt ggatctgctc tttccaggca gagtcggcat agttcaggaa
     1801 ccactgatca tccagaatct ttacataaac gcggttgccg caacggcaga tcacacgctt
     1861 ctcactcatg tcatggaaga tgatacttcc gcgagtgtcc acaaactccc tggttacgtc
     1921 ctcacgagcc tgacgaacag atttacctgc atgctctcca cagttttcat tcagttttcc
     1981 tttggagaac tctgcagtgt acaactcctg tgtcagctca tccatccggg gatcgtcctg
     2041 atgcggaatc ttattctttt ccacaatttc ctgtgccgga atttttccga agttgggaac
     2101 cgttaccaga ggaataggaa caatctctgt gtatttaccc tgctgctgaa gatcgcggag
     2161 tgcaatgtaa tcgaacggtg catgtgccgg aacactcatc accagacctg aacccatatc
     2221 tggatcaaca aatgatgcag ggaggattgg aactgctgca cccgaaattg gatgagttgc
     2281 tgctttgtca acaagttctg caccggagat tgtaccgatc tctgttactg tgtgtttctg
     2341 catccggagt ttttctgcag cttcccggct aatgatccat ttttctccgt caacctctgc
     2401 ttttaggtag gtgacctctg ggtttgccca aaggtttgtt actccgaaga tcgtctcagg
     2461 ccgaagtgtc gctgttggga taaagaactc gccgaatgga tacttcacaa acacgaagtg
     2521 catgatctca gcggaatctc cctctaaaag atcatgatct cccaccggct ggtcacagtt
     2581 tggacagtaa cgtaccggat actcaccctt gaccacttta ccctggccat ggatgtggga
     2641 gtactgccac tcaataaatt tactgtactg tggaatgatt gtcgtaaacc gccggcgcca
     2701 gtcgatcgaa agaccgagct gcttcatcac gcgttcatac tcatccgcga agtagttcac
     2761 gatcacaatt ggatcggtaa aggaatccag cgtctcctgc ggtacacggt aaagaccgcc
     2821 gtagagtttc agcgttttct catccttctt tgcaatccgc tttgcaatac caagcaccgg
     2881 tgcaccggta acatggaatg ccatcgggaa cagaacctgt ttcccttgca ttctccagaa
     2941 ccgtgcaacc acatcaggta caatataggt tctgccgtga cctacatgca ttgcaccgct
     3001 tggatagggg aatgcaacgt tgatgtagaa cttctcttta tcctctgcag gattcgactc
     3061 aaaggcaggg atccaagtgt tacggatctc ctgctcaatt tcactcatta caaactcttt
     3121 tgccaaattg accaccttat gcatatcgta ttattagtcc accggacgaa tcttcagctc
     3181 ttcacccgca ccatagcggc ggatcatctc cattgcaata ctgctgtttc gggcaaggtg
     3241 aatctcaccg cgtacatcaa ctgtcgcagt gaagagatac tcctttcctg agaagagatc
     3301 aacaatcttg ttgctgtact ctgggcagat tagggcaagc tgttttttct cacttctgat
     3361 cttgaatgcg ccggaggatc ctgatcctgc cggtactgcc ggagtagaga cctcccgtcc
     3421 cggaagatcg cttagcggac gaacgtcaat gccaactcca actttactga caactgcggc
     3481 gatgttcttt cctgcttttc cgattgctgc agggacatct gcgtcttcaa tatagacagc
     3541 tgccttggtg tcgttgatca ttctgacgat aatctcgcct tcggtgaatc gggagatctc
     3601 tttctgaatc tccttttcga ggatgatcca tgcggggttg tcttccgtga cagcataggt
     3661 tggcatactg tgaattggcg gcggaacatg tgatgcgtca tcaccatcgc catttacctc
     3721 agatgtggat tctgttaccg atgttgcagt tgtctcaata acttcagcag gcgttgcgtt
     3781 gattacctgc actgttgcag ccggtgtctc ggtctgttct gctgggtgga ttcctccgcc
     3841 ggaggtaaca gttacttctc cttcgtaacg gaatgcttca gctgcaacaa cgcctgtgat
     3901 gatgtttgtg aagcggacaa tgggtctgac ctgcgggtcg ccaatccgct ccagtgctgc
     3961 gggtactcca aagacagtac tgacattgta caaatcggag atgtcaccat ccttgacaaa
     4021 gaccaccgtt gccacgatct gcgggatgag gttgaggtca acaaggcttg caaggcggat
     4081 taatgcgtct ggagcagatc gtgcatgcag gccgccgatc actttaatgc cggaaagacg
     4141 gagatctgca aaaaccgcaa gctcttcgga tcttctcatc tcatcaaata ctacatagtc
     4201 cgggcggagc aaggtgagaa cctcacctgt tgcagcgata ctgccatcga gtgcagtgta
     4261 ctgcgtgatt ttgtccggca ccatgagatc gcgcggagat tcgatcgttt taacgatgta
     4321 gttttccgag gagaggtagg ttgcgatgtt ctgttccagg gttgatttcc cagatcccgg
     4381 agttccgacg atgagcattc cgccgttacc gatgttcaac cgttctttca gtgctgccgc
     4441 gtagttgtag gagtcaaagg agacctcgcg ggttggccgc acaattgtta tttcgattcc
     4501 gtccgagaat ggtggacgag ttgtggtgat gcgcatcgag ccgatctggg atacggtaac
     4561 accgggcatc tctacttcga caaatccatc tgggtggcgc ttggcgcgtt caagacattc
     4621 ctgtgcaatt gcccgcagtt cgtattcggt tgctggggtg tcgcgaatcg taacaagttt
     4681 tgattcacga attgttccct tgcgggcata gggtgggacc cgctctttga gatatacggc
     4741 aaaggtgttt tcatcgaaga atgcatcaat taacagcggg gcgaagttgt cggtgatttc
     4801 tggtctgagg aagacaacgt tgattccttt cgcacgcgca acctcggcct gaacatagtc
     4861 gcttgttagg aatgttgcat tgtgtgcgat ggcaacctcc cgtatcatgc tgtcaatctc
     4921 tcctccgcct gcgagtttga cctgttcaag tttggggcga acgcccacat attctaggtt
     4981 gatgagacct tcttccttca ttttgcagag tttctgcaac tctgagaggc cggaaaatcc
     5041 tatttctctt ccctggtttg cctgtgcttc gagttcggcg aagacggctt cgggtataat
     5101 tatcgttgcg ccacgatatt caccctgttc tataagggcg gtgacgcgtc cgtcgatgac
     5161 gacgcttgta tccggtacga gtatcataaa aaatctacca ttatgtatgg gttgtcaata
     5221 tcttattagg tctactgtat tttgtgatag atctcttgcg ggtctttgag tctggggagt
     5281 atgatccttc cgtcaatggt acggaaaaaa caactaaagt acccttcatg gcatgcacaa
     5341 cccgtttgtt ccacaagata gagaaggcaa tcgtgatcgc aatctacacg aatctcgcag
     5401 accttttgca ggtgtcctga ctcctctcct ttcttccaaa gtttctgcct tgaacggctg
     5461 tagtaatgag caaatcctgt ctctttggta agcgtaacgg cttccttatt tgcataggca
     5521 aacatgagga cagtttttgt ggcaatatcc tgaacaatta cgggaatgag accaccctcg
     5581 taggtaagat tgtcaagtaa atcggtcatt tctgctatgt atatggggtg gcggggaaaa
     5641 taaggagttt gggcatgtaa tctctctgag atggattttc atttctgatc acaaaagtca
     5701 gggctacgaa tttctctgct tattcacacc tttttatatc cgtctgtcaa taatataata
     5761 atacgatgag taagaatg
//
LOCUS       DALTZZ010000251         1953 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_42699,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000251 DALTZZ010000000
VERSION     DALTZZ010000251.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42699"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(45..1244)
                     /locus_tag="O0X12_04890"
     CDS             complement(45..1244)
                     /locus_tag="O0X12_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HJK53522.1"
                     /translation="MIDTGSVAWVLASTALVLLMTPALGLFYGGMVRKKNFLSVLMLV
                     FASLMLVIIQWFLLGYSLAFGTTQLGIIGGLEHLGFAGVGFDTLRGLEIPDLLFAAFQ
                     MTFAGLTLAIIVSGVAERIRFGAFLVFGILWTTLVYDPLAHWLWGGGWLAQLGALDYA
                     GGTVVHISAGFGALALAIYIGKRSGFGVHSLNPQNIPMTFFAGGLLLFGWMGFNGGSA
                     LAVNETAVHAIMVTLLSAAAGTISWMVVNWRHGKPSSLGFISGTIAGLGAITPAAAFV
                     DMWAALVIGVVAGVLCYYALLWRVKRSFDESLDAWAVHGVGGFWGTIACGIFAAASVG
                     GASGLLEGNAYLFGVQILDAVVTAAFAFAVTYVLAWVVDKTIGLRVSEDEEYIGLDLT
                     QHGEMVT"
     gene            1461..1751
                     /locus_tag="O0X12_04895"
     CDS             1461..1751
                     /locus_tag="O0X12_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53523.1"
                     /translation="MYEGTGVEMSSWVILGIVIIGCLVLIAVISLIEKKYNKRLISPR
                     QEMILENAKERAQEVFAEEKPPEFVVGQVYQMEDGTLAKYAEDGKFYKIKTK"
     gene            1761..>1953
                     /locus_tag="O0X12_04900"
     CDS             1761..>1953
                     /locus_tag="O0X12_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HJK53524.1"
                     /translation="MKKVKITVLKTTFDEDLAREYGVPGLGPCPMLTAGQVFYADYAK
                     PAGFCDEAWKAIYQYVFALA"
BASE COUNT          597 a          581 c          430 g          345 t
ORIGIN      
        1 ggattagata aatatggata attcccggat tatcccaaaa aaagttaggt aaccatctcg
       61 ccgtgctggg tcagatccag accgatatac tcctcatcct ccgaaacccg cagaccgatc
      121 gtcttgtcca caacccaggc aagaacatac gtaaccgcaa acgcaaacgc agccgtaacc
      181 accgcatcca gaatctgcac accgaaaaga tacgcattcc cttccagcag tccagacgca
      241 ccgccgaccg aagccgcagc aaaaataccg caggcgatcg ttccccagaa accgccgaca
      301 ccatgcacag cccaggcatc aagactctca tcaaaggaac gtttcaccct ccagagcaga
      361 gcatagtaac acagaacacc ggcaaccaca ccgatcacaa gagcagccca catatccaca
      421 aacgccgcag ccggagtaat tgctcccagt cccgcgatcg ttcccgaaat aaaaccgagc
      481 gaactcggct ttccgtgccg ccagtttaca accatccatg aaatcgtacc cgcagcagca
      541 gaaagcagtg tcaccataat cgcatgaact gcggtctcgt tcaccgcaag ggcactgccc
      601 ccgttaaagc ccatccagcc aaacagcaac agacctcccg caaaaaatgt catcggaata
      661 ttctgcggat tcaaactgtg cacaccgaac ccggaacgtt tcccgatgta gatggcaagt
      721 gccagagcac caaaaccggc actgatatgc acaaccgttc cgcctgcata atccagagca
      781 cccagctgtg ccagccatcc gccgccccac agccagtgtg caagcggatc atacaccagc
      841 gtcgtccaca aaataccaaa caccagaaac gccccgaacc tgatacgctc cgcaacaccg
      901 gagacgataa ttgccagcgt cagccccgca aacgtcatct gaaacgcagc aaacaacaga
      961 tcaggaatct ccaagccccg cagagtatca aacccgacac ccgcaaaacc gagatgttcc
     1021 agtccgccga ttatcccaag ctgtgtagtc ccgaacgcca gcgagtatcc aagcaaaaac
     1081 cactggataa tcaccagcat cagcgatgca aacacgagca tcagaacaga gagaaaattc
     1141 tttttccgca ccatgccccc gtagaacaga ccgagggccg gagtcataag cagtacaaga
     1201 gcagtggatg ccaaaaccca ggcaacagat ccggtgtcaa tcatggtatg ataacctgcc
     1261 tcatctttcc cagtccggac agagtatctg aagttcaagc actcaaatcc ccgtctgccc
     1321 gaaccacaga taatgcagta tcatgatctg tatttcaaat ataaaaacat aagacaatgt
     1381 ttagcatggc acatgttgac acacatagaa atatcgacga ttacttttat ccggcatcat
     1441 atcccatagg aaaggtagct atgtatgagg gaactggagt ggagatgagc agctgggtga
     1501 tcctcggcat tgtcatcatc ggctgtcttg ttttgattgc ggtcatctcc ctgattgaga
     1561 aaaaatacaa caaacggctc atcagcccgc ggcaggagat gatcctggaa aacgccaagg
     1621 aacgtgccca ggaggttttc gccgaggaga aacctcctga gtttgtggtg gggcaggtgt
     1681 accagatgga ggacggaacc cttgccaagt acgccgagga cggaaaattc tacaaaatca
     1741 aaaccaaata accgcaggca atgaaaaaag taaaaataac ggttctgaaa acaacctttg
     1801 atgaagacct cgcccgcgag tacggagttc ccggtcttgg cccctgtccg atgctgacgg
     1861 caggacaggt gttttatgca gactacgcaa agcccgcggg attctgtgac gaggcatgga
     1921 aggcgatcta ccagtatgta tttgcactcg ccc
//
LOCUS       DALTZZ010000252         1763 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_43201,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000252 DALTZZ010000000
VERSION     DALTZZ010000252.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43201"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="O0X12_04905"
     CDS             <1..301
                     /locus_tag="O0X12_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK53525.1"
                     /translation="LTTLIMSAAPASKQGMVSSLTGLERFAPMTIGVAVYNIIFMHGI
                     MAAVRNSGITEKPPGDIAAAVLSYGFDLAFVVSVILAVVLFVLCIFIREETAEEK"
     gene            460..1230
                     /locus_tag="O0X12_04910"
     CDS             460..1230
                     /locus_tag="O0X12_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HJK53526.1"
                     /translation="MDNNTVLLVTVPPMVEMVSTVAGDGFTVISVVPEGISPHTYEPT
                     PSDVSSFAAADMWFTLGEGFLPLEDQVAEALPDLPRVVTGTGIQAVAEAGSEEGETDP
                     HIWLSARNGILMTKAICDGLSAQYPDLAERFSAQADAFCAALSDVDARLADAAAAMEP
                     KAFLTTHGSFGYLAEDYNITQLVIAREGKEPAARELAEIVDSAKAAGVHIIVTEPLSG
                     ERTARVLSEELGIEPVQINPLSVHYMDTLNSLEEVLKG"
     gene            1227..>1763
                     /locus_tag="O0X12_04915"
     CDS             1227..>1763
                     /locus_tag="O0X12_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJK53527.1"
                     /translation="MSEVLEIDHLVVERSGHVVLDDVSFVLHQGDVAAIIGPNGGGKT
                     SFLMSILGLIQPKSGTIRVFGEEPAAARARIGYVPQVHTFDFWYPMTVQEMVLTGRLG
                     HIPGLVKRYHEKDYAAAKDALAAMDLTGYENRPIAELSGGEQQRAVISRALAGCPQLL
                     ILDEPTVYVDGPTGERLMT"
BASE COUNT          377 a          411 c          513 g          462 t
ORIGIN      
        1 actcacaacc ctcattatga gtgcggctcc cgcatcaaaa cagggcatgg tctcgagtct
       61 tacaggtctt gagcgttttg cgccgatgac aattggtgtt gctgtctaca atatcatctt
      121 tatgcacggg atcatggctg cagtccgtaa ctcaggtatt accgaaaaac ctccgggaga
      181 cattgccgct gctgttctca gttacggctt tgatctggca tttgtggtgt ctgtcatact
      241 ggctgtggtt cttttcgtgt tgtgcatctt tatccgggag gagacggcag aagagaaata
      301 accactgccg tcttttttga cgaaatcttt agcatcctaa acgcagaata ttcattatcc
      361 atacgggggt gttttgccgt tttcaaaata tgtcttgttc taagcgttgt tctggtgctt
      421 gccgcagttt cggcgggatg tatctccact cccgctgagt tggataacaa tacagtactg
      481 cttgttaccg ttcctcctat ggtggagatg gtctcgacgg ttgccggaga cgggttcacc
      541 gttatctcgg ttgtgccgga ggggatatct cctcacacat atgaaccaac cccttccgac
      601 gtgtcatctt ttgctgctgc ggacatgtgg tttacacttg gcgaggggtt cctgccgctg
      661 gaggatcagg tggcagaagc actgccggat ctgccgcgtg ttgtaacagg taccggaatt
      721 caggcggttg ccgaggccgg tagtgaggag ggagagacgg acccccacat atggctttcc
      781 gcccggaacg ggattctgat gacaaaagcg atctgtgacg gactttcagc acagtatccc
      841 gatcttgccg aacgattttc tgcccaagct gatgcgttct gtgctgcgtt gtctgatgtg
      901 gatgcccggc ttgcagatgc agctgccgcc atggaaccaa aggcgttttt gacgactcat
      961 ggatcgtttg gctatctcgc ggaagattat aacatcacac aactggttat tgcccgcgaa
     1021 ggaaaggagc ctgcagcccg tgaacttgcg gaaattgttg actcggcaaa agcggcaggt
     1081 gttcatatca ttgtgactga acctttgtcg ggagagagga ccgcgagggt actttccgag
     1141 gaacttggaa ttgagccggt acagattaac ccgctgtcgg tccattatat ggatacactg
     1201 aattctcttg aggaggtact gaaaggatga gcgaggtttt ggagattgat catctggtag
     1261 tggaacggtc cgggcatgtg gtgctggatg atgtgagttt tgttctgcac caaggtgatg
     1321 ttgcggcaat tatcggacca aacggagggg gaaaaacatc atttttgatg agcatccttg
     1381 gtctgattca gccgaaatca gggactatcc gtgtctttgg tgaagagcct gccgcagcac
     1441 gggcacggat tgggtatgtt cctcaagtac acacatttga tttctggtat cccatgacgg
     1501 tacaggagat ggttctgacc ggacgtctcg ggcatattcc cgggttagta aaacggtacc
     1561 acgagaagga ttatgcagcg gcaaaagacg cccttgcggc gatggatctg actgggtatg
     1621 agaaccgccc gattgccgag ctgtcagggg gcgagcagca gcgtgctgtg atctcccgtg
     1681 ctctggccgg ctgcccccag ctcctgattc ttgatgagcc gacggtgtat gtggacggac
     1741 ctaccgggga acggctgatg acg
//
LOCUS       DALTZZ010000253         1641 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_43374,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000253 DALTZZ010000000
VERSION     DALTZZ010000253.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43374"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1641)
                     /locus_tag="O0X12_04920"
     CDS             complement(<1..>1641)
                     /locus_tag="O0X12_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJK53528.1"
                     /translation="PEVISKYYGESEQRLREVFEEAEANAPAIIFIDELDSIAPRRED
                     VSGEVERRVVAQLLTMMDGITNRGQVVVVGATNRPDAIDPALRRPGRFDREIEIGVPS
                     EADRQEILQIHTRGMPFEGAAHIKELKLHGTEKKIEAAETEYNQNREKLLAQLASLSK
                     GFVGADLAALAREAAIRALRRQMNVIDLDTSRIPDETLRTLEVTAKDFAEASREIVPS
                     AMREIFIESVETRWTDVGGCETALNEVRESVEYPFTRKESFTQLGIRPPKGILLYGPP
                     GTGKTLIAKAVAHESGANFIAIKGPQLLSKWVGESERAVREIFRKARQVAPAIIFFDE
                     IDSLTPARNAGGEGNQVTENVLNQILTEMDGIEPLNDVVILAASNRPDIIDPALLRSG
                     RFDRLVYIPEPTIADRRAILAVHMRNMPLENSSLDAAAEQLAGRSEEKVRALIGKYTG
                     KTLTLRQIKSAAKKIEAEESGLSIPQLRRMLTDLFAAQNVIFEDTVRGEFIEKIAGAT
                     DGFVGSDLESLCREAAMEALRRNGTTVTDADFAAAKLR"
BASE COUNT          337 a          457 c          403 g          444 t
ORIGIN      
        1 cccgcagttt tgccgctgca aaatctgcat cagtgaccgt tgtaccattc cgccggagtg
       61 cttccattgc tgcttcgcgg cagagactct cgagatcgga tccgacgaac ccgtcggttg
      121 ctccggcaat cttttcgatg aactctcccc gtactgtatc ctcgaagatc acattctgtg
      181 cagcaaacag atccgtgagc atgcgtcgaa gctgtggtat ggagagtccg ctttcttcag
      241 cctcgatctt tttggcagca gatttgatct gccggagtgt cagtgttttt ccggtgtatt
      301 tcccaattaa tgcccgcacc ttttcctcac tgcgcccggc aagctgctct gcagcagcat
      361 caagagagga gttttcaaga ggcatattgc gcatgtgtac tgcaaggatt gcacgtctgt
      421 ctgcgatcgt tggctcagga atatacacta ggcgatcaaa tctcccggaa cgaagcagtg
      481 ccggatcaat gatgtccgga cggttggatg ctgcaagtat cacgacgtcg ttcagtggtt
      541 ctattccatc catctccgtg aggatctgat tcagtacatt ctctgtgacc tggtttcctt
      601 ccccgcctgc gtttcgtgct ggcgtgaggg aatcgatctc atcaaagaag atgatcgcgg
      661 gtgcgacctg tcttgctttg cggaatatct cacggactgc ccgttcagat tctccaaccc
      721 acttggagag cagctgtggc cctttgattg caataaagtt tgcaccgctt tcatgtgcaa
      781 cagcttttgc aatcagagtc tttcccgttc ccggcggtcc atagagcaag atgcctttgg
      841 ggggacgaat gccgagttgt gtaaatgact ctttgcgggt aaagggatat tcaaccgatt
      901 cgcgaacctc gtttagtgca gtttcacatc cacccacatc tgtccaccgt gtttccaccg
      961 attcaatgaa aatctcgcgc atggcagacg gtacaatctc acgtgacgcc tcagcaaagt
     1021 cttttgctgt tacttcaagt gttctcaggg tttcatctgg aatgcggctg gtgtcaagat
     1081 ctatcacgtt catctgacgg cgaagtgcac gaatcgccgc ctcacgggca agggctgcca
     1141 gatccgctcc cacaaaccct ttggacaggg atgcaagctg tgcaagaagt ttctcccggt
     1201 tctgattata ctcggtttcc gctgcttcga tctttttctc tgttccgtga agcttcagct
     1261 ctttgatgtg cgcggcccct tcgaacggca tcccgcgcgt atggatctgc aggatttctt
     1321 gtctgtccgc ttcggacgga acaccgatct ctatctcacg atcgaatctt ccggggcgcc
     1381 ggagcgcagg gtcgatcgca tccggcctgt ttgttgcccc cactacaaca acctgccctc
     1441 ggttggtaat tccatccatc atagtcagaa gttgggcaac cacacgtcgt tctacttctc
     1501 cggatacatc ttcacgccgc ggagcaatcg agtctagttc atcaatgaaa ataatcgccg
     1561 gagcgtttgc ttcagcctct tcaaagacct ctcgaagccg ttgttcggac tcaccataat
     1621 atttagagat gacctcggga c
//
LOCUS       DALTZZ010000254         2463 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_43806,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000254 DALTZZ010000000
VERSION     DALTZZ010000254.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43806"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /gene="mcrB"
                     /locus_tag="O0X12_04925"
     CDS             <1..1249
                     /gene="mcrB"
                     /locus_tag="O0X12_04925"
                     /EC_number="2.8.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="coenzyme-B sulfoethylthiotransferase subunit
                     beta"
                     /protein_id="HJK53529.1"
                     /translation="NVALEKISPLVNPAVKKIIDDTKRTVAVNIGGVQEGLKTGKIGK
                     HQQILGRELDLDIVGNIDAIEAKIKEYVSVDADNQAEIKRFNGGKLLLVKVPSARIAS
                     AATYDAALTSVAAATTYAVVEQFNVDMFNANTVKAAVFGSYPVTMDMAGGACSMIMSI
                     PQNNESLGYALRNIPANQSVMMTHRNAMQGAALAATFEQAGQFEMGNAIGPFERAQLL
                     LYAYQGLNANNIVYDLVKANGQTGTVGTVVQSLVERAIEDKVIVQGAGSATFKPYETK
                     DPMMWNAYASAGTLAATMVNCGAGRFAQAVSSTLLYFNDLLEHETGLPGADFGRTMGV
                     AVGFSFFSHSIYGGGGPGIFNGNHVVTRHAAGNGMPCIVAACALDAGTQMFSPEGTAK
                     IYGDTFGQIEEFARPIQAIAKAV"
     gene            1291..1764
                     /gene="mcrD"
                     /locus_tag="O0X12_04930"
     CDS             1291..1764
                     /gene="mcrD"
                     /locus_tag="O0X12_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase operon protein D"
                     /protein_id="HJK53530.1"
                     /translation="MTEPTYPQLRIVPMRFLNPETVEILLDRLFRVGGIRRLVLNGPS
                     IPATVPYGPARGTANPTTYRRTIQVCGEEYLLGVQVGTILLELEDSSLIPDIKAACDE
                     VFADKFPYGITEGIYMRSNMTTTDYAKYGIVDDVRILGMTDPKSKQRPIILQGNR"
     gene            1764..2435
                     /gene="mcrC"
                     /locus_tag="O0X12_04935"
     CDS             1764..2435
                     /gene="mcrC"
                     /locus_tag="O0X12_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-coenzyme M reductase I operon protein C"
                     /protein_id="HJK53531.1"
                     /translation="MPLGRVTQLVDCRQSMGMGKGGSLAQRGTISECKNPDVIIVGMS
                     PGRRHITKPVCDITSALRQQGIEYSVSTLVLNAGSGVPPDAEIGGVALGSNFGILDRE
                     IEQIERHKVAVLHHGNIRSHVIHKSRKILQECSVNAVVVCQAPVDFEDFAREGVKTAY
                     VMPKPDKIRTKGTVMGIVTGITRGQTPSRVAMADVIHEVLRIIKNPEMGLHETHESPV
                     SGITH"
BASE COUNT          661 a          636 c          617 g          549 t
ORIGIN      
        1 caacgtagca ctcgagaaga ttagcccgct ggtcaacccg gctgttaaga agatcatcga
       61 cgacaccaag agaaccgtcg cagtcaacat cggcggtgtg caggaaggac tcaagaccgg
      121 aaagatcggc aaacaccagc agatcctcgg ccgtgaactc gaccttgaca ttgtcggcaa
      181 cattgatgca atcgaagcaa agatcaaaga gtacgtttct gttgatgcag acaatcaggc
      241 agagatcaag cgcttcaatg gtggaaaact cctcttagtc aaggttccgt ctgcacgtat
      301 tgcaagtgca gcaacctacg atgcagcact tacctctgtt gcagcagcaa ccacctacgc
      361 agttgttgag cagttcaacg ttgacatgtt caacgcaaac actgtcaagg cagcagtctt
      421 tggttcctac ccggttacca tggatatggc aggtggagca tgttccatga tcatgtccat
      481 cccgcagaac aacgagtctc tcggatatgc acttcgtaac attccggcaa accagtctgt
      541 aatgatgacc caccgcaacg caatgcaggg tgcagcactc gccgcaacct tcgagcaggc
      601 aggtcagttt gagatgggta acgcaatcgg tccgttcgag cgtgcacagc ttctgctcta
      661 cgcctaccag ggtctgaacg caaacaacat cgtctacgac cttgttaagg caaacggcca
      721 gaccggaacc gtcggtactg ttgtccagtc tctcgttgag cgtgcaattg aggacaaagt
      781 cattgtccag ggtgcaggca gtgcaacttt caagccgtac gagaccaagg acccgatgat
      841 gtggaacgca tacgcatccg caggaaccct cgcagcaacc atggtcaact gtggtgctgg
      901 acgtttcgca caggcagttt cttccactct cctgtacttc aacgaccttc ttgagcacga
      961 gaccggactt ccgggtgcag acttcggaag aacgatgggt gttgcagtcg gtttctcctt
     1021 cttcagccac tctatctatg gtggaggcgg accgggtatc ttcaacggca accacgtcgt
     1081 aacccgtcac gcagcaggaa acggtatgcc ctgtatcgtt gcagcatgtg cacttgatgc
     1141 aggtactcag atgttctccc cggagggaac cgcaaaaatc tacggtgata ccttcggtca
     1201 gatcgaagag ttcgctcgcc caatccaggc aattgcaaag gcagtctaag cctctgtaac
     1261 aatttccgtc acgacggata ggtcaaaaga atgacagaac caacatatcc acagctgaga
     1321 atcgtcccga tgcgatttct gaatcccgag accgtcgaga ttcttcttga ccggctcttc
     1381 cgcgtgggag gtatccgccg tttagtctta aacggtccga gtattcccgc aaccgtacca
     1441 tatggtccgg ctcgcggtac tgctaacccg accacgtacc gcagaaccat ccaagtatgc
     1501 ggagaagagt atctgctcgg tgttcaggtg ggaacaattc tccttgaact ggaagactcg
     1561 tctctgattc ctgatatcaa agcagcatgt gatgaggtct ttgccgataa gttcccgtat
     1621 gggattaccg agggtatcta tatgcgttct aatatgacga cgactgatta tgcaaaatat
     1681 gggatcgtcg atgatgttcg tatactcggt atgaccgatc caaagagcaa acaacgcccg
     1741 atcatccttc aggggaacag ataatgcctc ttggacgtgt aacacaactg gtcgactgca
     1801 gacagagtat gggtatgggt aagggaggct ctcttgctca gaggggaacc atctctgaat
     1861 gtaaaaatcc tgatgtcatc atcgtcggta tgtcacccgg cagaaggcat ataacaaaac
     1921 ctgtctgcga tatcacgtcg gcactgcggc agcaggggat cgaatacagc gtaagtacct
     1981 tggtgctgaa cgcaggcagc ggagttccgc cggacgcaga aatcggtggt gttgcactcg
     2041 gttccaactt tggcatatta gatcgcgaaa ttgaacagat agaacgtcac aaagttgccg
     2101 ttcttcacca cggaaacatc cgatcccacg taatccacaa atctcggaag atcctgcagg
     2161 aatgcagcgt caacgctgtt gttgtctgtc aggcaccagt tgattttgag gatttcgcac
     2221 gggaaggcgt caaaaccgca tacgtcatgc caaaacctga taaaatcaga acgaaaggaa
     2281 cagtcatggg gattgtcacc ggaatcaccc gaggtcagac tcctagccgt gttgcgatgg
     2341 ctgatgtcat tcatgaagta ctacgaataa tcaaaaatcc tgaaatggga ttacatgaaa
     2401 ctcatgaatc tcctgtatca ggaattacac actaaggtga acaaactatg gcatatacac
     2461 cac
//
LOCUS       DALTZZ010000255         2256 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_43947,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000255 DALTZZ010000000
VERSION     DALTZZ010000255.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43947"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="O0X12_04940"
     CDS             complement(<1..281)
                     /locus_tag="O0X12_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HJK53532.1"
                     /translation="MKLRQLEMQLQKVRGFSHPSAEAEQYMTPAPLAARMLFHAAMNG
                     DIVGIRVCDLGCGTGMLSIAAALLGADVIAVDFDPGALAVARENAELFDV"
     gene            complement(285..1232)
                     /gene="dph2"
                     /locus_tag="O0X12_04945"
     CDS             complement(285..1232)
                     /gene="dph2"
                     /locus_tag="O0X12_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HJK53533.1"
                     /translation="MIPLSDLIDDLHARGAKKVAVQLPEGLKRGTAMLARALRDEGFE
                     VMVCGDACWGACDLALDALADADVLVHVGHTPVTPEKNVIYIPFRQDIDLEILHSAVS
                     PLKNFSTVGLVTTIQHAHQTREVADWLQDHGIHVVVGEGSDRTPEPGQVLGCTYAAAK
                     SAGADAYLFVGTGVFHAIGVSLATGKPTFALDPYGDGGVQEVSADRLLRKRFVQIEKA
                     KSAKTFGILLSSKSGQCRKELAKRLETLSPDASVVLIREVSEMQLRNLGFDAYVNTAC
                     PRLALDDQSRFPMPVLSPVEFEIVLGLRSWDDYTIDEIE"
     gene            1485..>2256
                     /locus_tag="O0X12_04950"
     CDS             1485..>2256
                     /locus_tag="O0X12_04950"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HJK53534.1"
                     /translation="MKAVLGLEDGTIITGSGFGVEGAATGELVVTLVSNGYMEALSDP
                     SVAGQLLMFGYPLVGNYGANPDQLQSSKVHATGAIVRELCEFPKHEPTMAAYFEENGL
                     MGIEGVDTRMLTIKLREEGVMRAALLTGSDDGHEAVRLAQNAPVQETRELIPEVTCKE
                     AYHIPGSGKKIAVLDLGCRKSILKSLQNRGADLYVYPYGTPADVILADKPQALFLTNG
                     PGYPFAAPKAIETVKNILGTIPVQGVCMGTEIIAAALGG"
BASE COUNT          597 a          627 c          529 g          503 t
ORIGIN      
        1 acatcgaaca actccgcatt ttcccgtgct actgcaagag caccaggatc aaaatcaacc
       61 gcgatgacgt cagcaccaag cagagcggca gcaatggaaa gcatacctgt accgcatcca
      121 agatcacaga cacggatccc aacaatatcc ccgttcattg ctgcgtgaaa cagcatgcgg
      181 gctgcaagcg gtgcaggcgt catgtactgt tctgcttcgg cagacggatg tgaaaatccc
      241 cttacctttt gcagctgcat ctcaagctgg cgaagtttca tggtttactc tatttcatcg
      301 attgtgtagt catcccatga ccggagcccg agtactattt caaactccac aggagacaga
      361 accggcatcg ggaaccggct ctgatcatcc agggcaaggc gcggacatgc ggtgttcaca
      421 tatgcatcaa aaccaaggtt tcgcagctgc atctccgaca cctcacggat gaggacgact
      481 gacgcatcag gagaaagggt ctccagccgc tttgcaagtt cttttctgca ctgtcccgat
      541 ttgctggaaa gcaggatacc aaatgttttt gccgatttcg ctttttcgat ctgcacgaac
      601 cgtttccgga gcagacgatc cgccgaaacc tcctgaacac ctccgtcacc ataaggatcc
      661 aacgcaaaag ttggttttcc tgttgcaagc gagacaccga ttgcatgaaa gacaccggtt
      721 ccaacaaaca ggtacgcatc tgcacccgcg gattttgcag ctgcatacgt acatccaagc
      781 acctgtcccg gctccggagt gcgatcagat ccctcgccaa ccaccacatg gattccgtga
      841 tcctgcagcc agtcagccac ctcccgtgtt tggtgtgcgt gctggattgt ggtcaccaga
      901 ccaactgttg agaaattctt gagaggagac acagcagaat ggagaatctc cagatcaata
      961 tcctgacgga acggaatgta gatcacattc ttttccggtg ttaccggcgt gtggccgaca
     1021 tggaccaaca catcagcatc cgcaagagca tcaagtgcca gatcacacgc cccccagcac
     1081 gcatcaccgc agaccatcac ctcaaaccct tcgtcacgga gtgctctggc aagcattgca
     1141 gtccctcgtt tcagcccttc cggtagctga actgcaactt tttttgcacc acgtgcatgg
     1201 agatcatcaa tcagatcaga aagcggaatc acagatcaca cccccaaaaa acaccggaca
     1261 gtcgcatagt aatactatac tacttgtgtc ggaaagggaa acacctatcg gaaaaacctg
     1321 atttgcggcg ctggaaaaat atatttattt gaggatatct atttagatat attataaaat
     1381 atataatatt tcgaatagac acatatttta ttcgattgaa tatatatgaa tcatagatta
     1441 cccttattac ctcccgacgc ggacatataa gatggtacta aatcatgaag gcggtcttag
     1501 gacttgaaga tggaactatc atcactggaa gcggcttcgg tgtcgagggc gctgcaaccg
     1561 gtgaactggt agtaaccctc gtatccaacg gatacatgga agccctcagt gacccaagtg
     1621 ttgccggaca attattaatg ttcggttatc cacttgtggg aaactacggc gcaaaccccg
     1681 accagctcca gagcagcaaa gtgcatgcaa ccggcgccat tgtccgtgaa ctttgtgagt
     1741 tccctaagca cgagccaacg atggctgcgt atttcgagga gaacggcctc atgggaatcg
     1801 aaggagtcga tacccggatg ctcaccatca aactcagaga ggaaggtgtc atgcgtgcgg
     1861 ctcttcttac cggctccgat gacggtcatg aagccgtccg ccttgcccag aatgctcctg
     1921 tgcaggagac ccgcgaactc attccggaag tcacctgcaa agaagcctac cacattcccg
     1981 gttccggcaa aaagatcgca gttcttgatc tcggctgccg gaaatccatt ctgaaaagcc
     2041 tgcagaaccg cggtgccgac ctctacgtct acccctacgg aacgcctgcc gatgtgattc
     2101 ttgcagacaa accgcaggca cttttcctga ccaacggtcc ggggtacccg tttgccgcac
     2161 cgaaagccat tgaaaccgtc aaaaatattc tcggtacaat tcctgtacag ggtgtctgca
     2221 tgggaaccga gatcattgca gctgctctcg gcggtg
//
LOCUS       DALTZZ010000256         3011 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_44084,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000256 DALTZZ010000000
VERSION     DALTZZ010000256.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44084"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(12..1397)
                     /locus_tag="O0X12_04955"
     CDS             complement(12..1397)
                     /locus_tag="O0X12_04955"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK53535.1"
                     /translation="MDSRTKRLILFAASIGAFLNPLIGSMIILAMPEVGVAFEVSARD
                     LGWLSTIFILANAIFLVPASRLSDLIGYKRSYLLGAAIVAVACGLSVFAPTYTILLLL
                     RVIAACGTSFLMITGLAILSYVYPPNERGAAFGINTAMVYIGASAGPILGGFLTGIFG
                     WRAVFLVMVPLAVATAVPMWQFFRDEIKIPSDSPFDVRGTILYAVAMFCTMYGLSTLP
                     DLLSAVLTVIGIILMAAFVWYELRIPSPVLHVKLFFTNQRFARSSYAALLNYGCTYGS
                     VFFVSLYLQSVGQLTAAEAGMILFFQPLIQAIMTPIAGRFSDRIDPRYIVTIGMILSG
                     IGVLLLAGLDTGTDLHYIAVTQVFIGLGSALFSAPNTNAIMGSVPAEEYSTASGIVAV
                     MRQAGMIISMAVCMSAISVYVGGTDMLGPEMYQEFLQALKVSMIFCAVLAFVGVLFSW
                     FRGKGTVSDAS"
     gene            complement(1403..2059)
                     /gene="nth"
                     /locus_tag="O0X12_04960"
     CDS             complement(1403..2059)
                     /gene="nth"
                     /locus_tag="O0X12_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HJK53536.1"
                     /translation="MNTVEAVSVLTELNRIYPHTREDMNFLKFENPYQILIMTILSAQ
                     TTDATINGLRDELFSCYPDPQTLAAAEQTEVERIIHPAGFYHAKAKNIIGTAKCLCEN
                     FGGEVPQTIDELVTLPGVGRKTANIVTNHAFGNPVGIAVDTHVGRLSQRIGFSDNSDP
                     NKIEQDLMKLFPKTWWGELNYLLISHGRAVCTAKKPACERCAISHLCRWHGKMQTKQA
                     "
     gene            complement(2067..2141)
                     /locus_tag="O0X12_04965"
     tRNA            complement(2067..2141)
                     /locus_tag="O0X12_04965"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2103..2105),aa:Pro,seq:tgg)
     gene            complement(2321..2809)
                     /locus_tag="O0X12_04970"
     CDS             complement(2321..2809)
                     /locus_tag="O0X12_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53537.1"
                     /translation="MGFRSVFGHKLDELIEAEQQHEVLCKEFEEYILEIFPDAFAIER
                     PDADGDIVPDFLVTDTSSGKRFAVVCVFQPKFKTPEDGSCDILECGTFEQVRAYKQFA
                     HFERMPFFLIVGVGGLPDHPDRVFSIPVEEIGCSELPKEACLMYERHPSEIFTYREDH
                     LA"
BASE COUNT          795 a          761 c          755 g          700 t
ORIGIN      
        1 atcccaaatc cttagcttgc gtcagacact gtacctttac cacggaacca ggaaaagagt
       61 actcccacaa atgcaagtac cgcacagaag atcatcgaaa ccttcagtgc ctgtaagaac
      121 tcctgataca tttctggtcc gagcatatcc gtcccgccca cgtacaccga aatcgctgac
      181 atacagaccg ccatcgagat gatcattcct gcctgccgca tcacagcaac gattcctgat
      241 gctgtactgt actcctctgc cggtactgat cccataatcg cgtttgtgtt tggagcggag
      301 aacaacgctg atccaagacc aataaacacc tgggtaactg caatgtaatg cagatcggtt
      361 cccgtgtcca gtcctgcaag caggagaacg ccgattccgg agagtatcat accgatggtg
      421 acaatgtagc gggggtcgat cctgtcggag aatctccctg caatcggtgt catgatcgcc
      481 tgaataagcg gctggaagaa gaggatcatt cctgcctctg ctgcggtaag ttgtccgacc
      541 gactgcaggt acagactcac gaagaagacc gacccgtagg tacagccgta gttcagcagg
      601 gctgcatatg aggatcgggc aaaccgctgg ttcgtaaaga acagtttcac atgcagcaca
      661 ggcgacggaa tccgcagttc ataccagaca aatgctgcca tcaaaataat accgatgacg
      721 gtcagcacag ccgagagcag atccggcagt gtggaaagac cgtacatcgt acaaaacatc
      781 gcaaccgcat acaaaatcgt tccgcgaaca tcgaacgggc tgtcggacgg tatcttgatc
      841 tcatcacgga agaactgcca catgggaact gccgtagcaa ctgcgagcgg aaccatcacc
      901 agaaacaccg cccgccatcc aaagatccca gtcagaaaac cgccgaggat agggccagcg
      961 gatgctccga tgtacaccat cgcggtgttg atgccaaacg ctgccccccg ctcattcggc
     1021 ggatacacat aggagagaat ggcgagtccg gtaatcatca ggaatgaagt gccgcaggct
     1081 gcaattaccc ggagaagcag aagaatcgta taggtcgggg cgaatactga cagaccgcag
     1141 gcgacggcaa caattgctgc accaagcaga taggatcgtt tgtatccgat cagatccgac
     1201 aggcgcgatg ccggaaccag aaagatggcg tttgccagaa taaagatcgt ggacagccag
     1261 ccgagatcac gtgccgagac ctcaaatgcc acaccaacct cgggcatggc aagaataatc
     1321 atcgatccga taagcgggtt taaaaatgcg ccgatggagg ctgcaaataa aatcagccgt
     1381 tttgtacgtg agtccatctc tgttacgcct gttttgtctg cattttcccg tgccacctgc
     1441 agaggtgaga tatggcacac cgttcacatg cgggtttttt tgcggtacat accgctcgtc
     1501 cgtgactgat taagagatag ttgagttctc cccaccatgt cttgggaaac agtttcatta
     1561 aatcctgttc gattttgttc ggatcgctgt tgtcggaaaa tccgatacgc tgcgacagac
     1621 gtcccacatg ggtatcaact gcaatgccga ccgggttgcc gaacgcatga tttgtcacaa
     1681 tgtttgcggt cttacgtcca actcctggca gcgtgacaag ttcgtcaatg gtctgcggga
     1741 cttctccgcc gaagttttca cagaggcatt ttgctgtacc gatgatgttt tttgctttgg
     1801 cgtgatagaa tccggcggga tgaatgatcc gttcaacctc tgtctgctcg gctgcggcga
     1861 gtgtctgagg gtccggatag caggagaaca gttcgtcccg cagaccgttg atggtagcgt
     1921 cagtggtctg tgctgacaaa atcgtcatga tcagaatctg atagggattt tcgaacttga
     1981 ggaagttcat gtcctctctc gtatgcggat agatacgatt taactcggta agaacagata
     2041 cagcttcaac ggtattcata aaagaatggg gcagcccaga ttcgaactgg ggtcaaagcg
     2101 tcccaaacgc tctaggatgg accaggctac cctactgccc cgcttggtgc agtatatatt
     2161 ggtatgatac ccttaaaaaa ggataggata tttctctggt aatccggggg ggtggtcacc
     2221 aaatattcct ttcctgcggc atactgaatt cagtcatatt gtcataggca tcggctcatg
     2281 aatggtaaca ctgttgcagt gtcctgtgga aaaaagatgg ttatgccaga tggtcttcac
     2341 ggtacgtaaa gatctcggag gggtgtcgct cgtacatgag acaggcttcc tttggtagtt
     2401 cgctgcatcc gatctcctcc accggtatcg aaaacactcg gtcaggatga tccggaagac
     2461 cgccgacccc aacgataagg aagaaaggca ttctttcaaa gtgggcaaac tgtttgtatg
     2521 cccgtacctg ttcaaatgtt ccgcactcaa gaatgtcgca tgagccgtct tcgggtgttt
     2581 tgaacttcgg ctgaaacaca caaaccacag caaatcgttt tccggaggat gtatcagtta
     2641 ccaaaaagtc tggaacgatg tctccgtcag cgtctggccg ttcgatcgca aacgcatcgg
     2701 gaaagatctc aaggatgtac tcctcgaact ctttgcagag cacttcatgc tgctgttctg
     2761 cctcaatgag ttcatcgagt ttgtgtccaa atacgctccg aaatcccatg catctaactt
     2821 acacgggttg ctattttaag attgtgtcct gtaacccgtc aaatcatgtt gtttattcta
     2881 cgcgcgaatg aagaaaaacg atggaaatac tcaggaggac acagtctgtt gttgtttcct
     2941 caaattcaca gcagtaaagc cggattcctg ttttcgacaa atgcagaaca ggatgttaac
     3001 gatcacagaa c
//
LOCUS       DALTZZ010000257         1786 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_44133,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000257 DALTZZ010000000
VERSION     DALTZZ010000257.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44133"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="O0X12_04975"
     CDS             <1..413
                     /locus_tag="O0X12_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HJK53538.1"
                     /translation="MILNDHEPCRWEMNVETPEDLDARVIRSMQGEVDIPEFGINIRP
                     GPACSGFVSNIEGVLLRAEDAIRRALPSCEGDEITTAYELLEKIDQARASQFPFTVII
                     SDPSGNSGIVSPKAVKTKLDVEPQPDGCTIHPLA"
     gene            429..>1786
                     /locus_tag="O0X12_04980"
     CDS             429..>1786
                     /locus_tag="O0X12_04980"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJK53539.1"
                     /translation="MDSGAKRHLLTDAEETRLRKLIENRSSDSRLDSLTLAYIEKISG
                     KKWKDESVLEKIRAAITVQKDSYWKEGERRVVQYKGGYSVLGYMAYQMPGYVAEFTEF
                     FVRLLREGLIRDHVRILDVGAGPGTVAVAVARVLEMCDGITAEVVSLERSEVFREAYM
                     AVVPAFVKDAGDHVTAPRPIAADITETMPEGEFDLIVCSNMINELAVYGDEKTDLMMQ
                     LSTHLVADGNLILLEPADLANATMLRDLSRDLKRRGLTLYAPCNDIRGVHCSVSPCWT
                     FATYADIRPTKLMFALGGDTEKFRFVNTDVKFSYAILRKDGHRRCGYRVPAAAKRARL
                     AQLKKHVGKRIHVTVSVMSADIGDAKNYLYLVCDGTGEVPVYVALPAHHRNPEHEALL
                     SAPYGSVVAIDSVLVRFNPKQNAYNLLMAPESFTRLIVGVAGPSAPDKIAALKEKYGR
                     GSP"
BASE COUNT          403 a          421 c          522 g          440 t
ORIGIN      
        1 cgatgatctt aaacgatcat gagccctgcc gctgggaaat gaacgtggaa acacccgaag
       61 atctggatgc ccgcgttatc cgcagcatgc agggtgaggt cgacattccg gagtttggga
      121 taaatatccg tccgggtcct gcatgcagtg gttttgtgtc caatattgaa ggggtgctgc
      181 tgcgtgccga ggatgcaatc cgccgtgctc ttccctcctg tgagggggat gagattacca
      241 ctgcatatga actgctggaa aaaattgatc aggctcgtgc ctcacagttc ccctttaccg
      301 tgatcatttc agatccctcg gggaattcgg gcattgtctc gccgaaagcg gtgaaaacaa
      361 aacttgatgt ggagccgcag ccggacggat gtacgattca tcctctggca taaaccgttt
      421 ttgaatttat ggactctgga gcaaaacgcc atctgctgac ggatgccgaa gagacgcgtc
      481 tgcgcaaact gatcgaaaac cgatcatccg attcccgtct tgattcgctg accctcgctt
      541 acatcgaaaa aatttcgggt aagaaatgga aagacgagag tgttcttgaa aagattcgtg
      601 cggcaattac agtccagaag gactcctact ggaaggaagg tgaacgccgc gtggtgcagt
      661 acaagggtgg ctacagtgtg ctgggttaca tggcatacca gatgcccggg tatgttgcgg
      721 agttcacgga gttttttgtc cggcttctcc gtgagggact gatccgggat catgtccgta
      781 ttctggatgt gggcgctggt cccggcactg ttgcggttgc ggttgcccgc gttctggaga
      841 tgtgtgacgg tatcaccgca gaggttgttt cgctggaacg ctcggaggtg ttccgggagg
      901 cgtacatggc ggttgtgcct gcgtttgtaa aagatgcagg tgatcatgtt acagctccgc
      961 ggccgatcgc tgctgatatt actgagacaa tgccggaggg agagttcgat ctgatcgtct
     1021 gctcgaatat gataaatgag ctggcggttt atggggatga aaaaacagat ctgatgatgc
     1081 agctttcaac gcatctggtc gcggacggaa acctgattct gctcgaacct gcggatcttg
     1141 caaacgcaac catgctgagg gatctttccc gtgatctgaa acgccgcggt cttacactgt
     1201 atgcgccgtg taatgacata cggggagtac attgcagtgt ctctccctgt tggacgtttg
     1261 cgacatacgc agacattcgg ccgacaaaac tgatgtttgc attaggcggt gatacggaaa
     1321 aattccggtt tgtgaacacg gacgtgaagt tttcatatgc tattcttcgc aaggacggtc
     1381 accggcgctg cggataccgt gttcctgcgg cggcaaaacg tgcaaggctt gcacagctga
     1441 aaaagcacgt cggtaaacgc attcacgtca ccgtgtcggt gatgtccgcc gatattggcg
     1501 atgcaaagaa ctacctgtat ctggtctgcg acggaacagg tgaagttcct gtgtatgttg
     1561 cccttcctgc gcatcatcgg aatccggagc atgaggcact gttgtccgct ccctatggtt
     1621 cggttgttgc aattgactcg gttctggttc ggttcaatcc aaaacaaaac gcctataatc
     1681 tgctgatggc accggaaagt ttcacgcggc tgattgtggg agtggcaggt ccgtccgccc
     1741 ctgacaagat tgccgcacta aaagagaagt atgggcgggg tagtcc
//
LOCUS       DALTZZ010000258         3245 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_44253,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000258 DALTZZ010000000
VERSION     DALTZZ010000258.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44253"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="O0X12_04985"
     CDS             complement(<1..1164)
                     /locus_tag="O0X12_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HJK53540.1"
                     /translation="MRFLQLVSSAEAESTLLALGQPLPEELVPLAESVGRILSRRIVC
                     PEDIPGFHRSVKDGYAVRSVDTLGAGEQKPKLLKLTGRILMGQNASGMLEEKTTKYIP
                     TGGVMPIGADAVVMQESTELAGDMVLIKGPVSPGADVLRADEDFAKGEVVLEAGTKLT
                     AQATGVLAAFGIDPVPVVRRARVGILSTGNELVAPAATRSPGQIRDANSTLLISFMRE
                     AGAVPVFYGIIPDDASSLLPVLKTAASECDLVVVSGGSSKDERDVTAGVVAELGVIHV
                     HGVSIAPGKPTIIGSIGATPVLGLPGHPAATYMITSVFAGPLLRKMSGEIISQRVISA
                     PLSMSFPSEKGREDLVRVRILSDGRAEPILGKSGLLNTLVRSDGYIRVPAGLDG"
     gene            1365..2162
                     /gene="modA"
                     /locus_tag="O0X12_04990"
     CDS             1365..2162
                     /gene="modA"
                     /locus_tag="O0X12_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HJK53541.1"
                     /translation="MTRVSGLILLTLAVLAAFICAAGCIGTPTDEENATLLIYAGAGL
                     KAPMAEIGTVFGEQNGVTVELTYGGSGVLISQMETSHLGDAFIPGGQPDYQIAVNKGL
                     VSNDSQLVAYHVPIIAVQKGNPLGIQTVADLAKPGVRLALGDVNATAIGKAGVEIFKT
                     YGVSEEIEDNVILRGATINEVVTAMATGNADAALLTLDNAKGDQFDLIEIPVTNNSIL
                     IAPIGITTFTTQPELAQKFADFVASDEGKAIFEKYGFPAYPNEKYMS"
     gene            2169..2651
                     /gene="tsaA"
                     /locus_tag="O0X12_04995"
     CDS             2169..2651
                     /gene="tsaA"
                     /locus_tag="O0X12_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HJK53542.1"
                     /translation="MEETYTIRAIGKLRTPYTDMMQTPVQSQCSVGRGTIEVNPEYAE
                     GLDQIDTFSHLMLFVRLDRAPAELLAEKPMVDGGASHGIFATRHMSRPNKIGFSVVRL
                     LARRDNMLLIDGVDFLDGTPVLDIKPYIPAFDAVPDASSGWLTDQHLANIAEKSKNLS
                     "
     gene            2748..>3245
                     /locus_tag="O0X12_05000"
     CDS             2748..>3245
                     /locus_tag="O0X12_05000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HJK53543.1"
                     /translation="MFPTPDALFAALTSGEIWFAVQLSLTTAVISTLCCVAVAVPVAY
                     VMTRTPFAGKRWINILLNLPLSLPPLVAGVALLIFLSPVTSPVGQLLSGFGIFIVYTP
                     VAIVIAQFFVNLPYMIRIARSTFAMISHRYEHVARTLGCSEWRAFSQVTLPMAKSGLA
                     AGIVIT"
BASE COUNT          857 a          899 c          736 g          753 t
ORIGIN      
        1 gccatccaga ccggctggaa ctcggatgta gccgtcactt cgtactagcg tatttaatag
       61 accagacttc ccaagaatcg gctctgcccg gccatcggaa agaatccgga cacgtaccag
      121 atcctccctc cctttctctg acgggaaact catcgagagc ggggcagaaa taacccgctg
      181 cgaaatgatc tcaccggaca tcttccggag cagcggaccg gcaaacacag acgtgatcat
      241 gtacgtcgcc gccggatggc ccggaagtcc gagtaccggc gttgcgccaa tagatccgat
      301 aatggtaggt ttgcccggag caatcgaaac cccgtgcacg tgaatgacac caagttcggc
      361 aacaacacct gctgtaacat cacgttcatc cttggaacta ccacccgata caaccaccag
      421 atcacactct gaggcagcgg tcttcagtac cggcagaaga gatgatgcat cgtctgggat
      481 gatgccgtaa aaaaccggca ctgcacctgc ttcacgcata aaagaaatca aaagcgtact
      541 gtttgcatcc cttatctggc ctgggctccg cgttgcggca ggtgcaacca gttcattacc
      601 ggttgaaaga ataccaaccc gtgcccggcg caccactggt accggatcaa tgccaaacgc
      661 tgcaagaaca ccggtcgcct gagcagtcag ctttgtaccg gcctccagta ccacctcacc
      721 cttggcaaaa tcctcatctg cccgaagcac atcagccccc ggggaaaccg gccccttgat
      781 cagaaccata tcaccggcca gctcagtact ctcctgcatc accaccgcat cagcccctat
      841 tggcatcaca ccgccggtcg gaatatactt cgttgttttc tcctcaagca tacctgacgc
      901 attctgtccc atcaaaattc tgcccgtaag cttcagaagt ttcggtttct gttcccctgc
      961 acccaaggta tccacagacc ggactgcata tccgtctttg accgagcgat gaaaaccggg
     1021 tatatcctct gggcacacaa tcctgcgtga gagaatgcga cctaccgact ccgcaagagg
     1081 gaccagttct tccggcaaag gctgtcccaa agccagcagg gtactttccg cctccgcaga
     1141 ggacaccagt tgtaaaaatc tcatacaacc ttcctgcaca taatcccgga gacaggatac
     1201 ttcatacctc tcccagtggc ttcaaatatc atatctaaca acatggttga caaaattaat
     1261 aatgattagt attttagtgg attgaacata gttaatttaa taaatttaac attttcaaaa
     1321 agattagata cagttattat ggaggatcct catatggtaa tcctatgaca cgtgtatctg
     1381 gactgatact cctgactttg gcagtgcttg cagctttcat ctgcgctgca ggctgcatcg
     1441 gcactcccac agatgaggaa aacgcaactc tcctgattta tgcaggagca ggactcaaag
     1501 cccccatggc ggaaattgga acagtgttcg gggaacaaaa cggcgtgacc gtagaactga
     1561 cctacggggg atcaggtgtt cttatctctc agatggaaac ttcccacctc ggtgatgcgt
     1621 tcattcctgg cggacagccc gactatcaga tcgcggtaaa caaaggcctt gtctcaaatg
     1681 actcacagct tgttgcttac catgttccaa ttattgccgt acagaaaggt aacccacttg
     1741 gcatccagac cgtagcggac ctcgcaaaac cgggagtacg gcttgcactg ggtgacgtta
     1801 acgcaactgc aatcggaaaa gcaggcgttg agatcttcaa aacatacgga gtaagtgaag
     1861 agattgagga caacgtgatt cttcgtggag caaccatcaa cgaagtggta accgcaatgg
     1921 ccacaggaaa tgcggatgct gcactgctca ctcttgacaa cgcaaagggt gatcagttcg
     1981 acttaattga aattccggtg acgaacaact cgattcttat cgcaccaatt ggcattacaa
     2041 cctttaccac acagcccgaa ctcgcccaga aatttgcaga ctttgttgca tctgatgaag
     2101 gtaaagcaat ctttgagaag tatggcttcc ccgcttaccc gaacgaaaag tacatgagct
     2161 gatcaaagat ggaagaaaca tacacgatca gagcaatagg aaaactccgg acgccctata
     2221 ctgacatgat gcagacgccg gtacagagcc agtgttctgt cgggcgcggt accattgaag
     2281 taaatcccga gtacgctgag ggtctcgatc agatcgatac tttttcgcat ctgatgctct
     2341 tcgtaagact tgaccgtgct cccgcagaac tgctcgcaga aaaaccaatg gtggatggag
     2401 gggcatccca tggtattttt gcaacccgcc acatgtcacg gccaaataag atcggattct
     2461 ccgttgtccg cctactggca cgcagggaca acatgctgct gatagatggc gttgactttc
     2521 tggacggaac accggtactt gacatcaaac cctacattcc agcatttgat gcagtcccag
     2581 acgcgtcttc cggctggctg accgatcagc atctggcaaa cattgcggaa aagagcaaaa
     2641 acctctcgta aactatgcga aaaattccct ctttttttca gacaatctgt ttagcactga
     2701 cagcgatcgc agtagtagta ctcatagctg tttttggtgg cctgatagtg tttccaactc
     2761 cagatgcact atttgcagca ctaacctcag gagagatatg gtttgcagta caactctcac
     2821 tcacaaccgc agtcatctcc accctctgct gtgtagcggt ggcagttcct gtcgcatatg
     2881 taatgacacg aacaccattt gccggaaaaa gatggatcaa cattcttctc aacctcccgc
     2941 tgtcactccc gccgcttgtg gcaggagttg ccctgctgat ctttctctca ccggtcactt
     3001 ctccagtagg acaactcctt tcaggttttg gtatcttcat tgtgtacaca ccggtcgcaa
     3061 ttgtgatcgc acaattcttt gtaaacctcc cctatatgat ccggatagcc agatccacct
     3121 ttgcaatgat cagtcaccgg tatgaacatg ttgcacggac actgggctgt tctgaatggc
     3181 gggcgttttc acaggtaact cttccgatgg caaaaagcgg acttgcagca ggtattgtca
     3241 tcacc
//
LOCUS       DALTZZ010000259         1705 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_44389,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000259 DALTZZ010000000
VERSION     DALTZZ010000259.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44389"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            526..900
                     /locus_tag="O0X12_05005"
     CDS             526..900
                     /locus_tag="O0X12_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53544.1"
                     /translation="MGILTTLIQGLSAVPALLLLGILSPAVLCLVLITLPATPFLQTL
                     RRISLLLAGVNLVLGLCIPAYMDAAVAAAGGMTTGEAVIVISIVLILSVLTPVYGIIY
                     LFGGVRALRSVPSPQSSAQTDP"
     gene            complement(854..>1705)
                     /locus_tag="O0X12_05010"
     CDS             complement(854..>1705)
                     /locus_tag="O0X12_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HJK53545.1"
                     /translation="VIWVFLIGFAVTLLLLVKQVKGALLIGIAAAAVVGIPLGVTESA
                     NVISISEAWAALPSTFGVIFTDAGLVSLFSDPMRLPLVLVTILAFGLCDMFDTIGTLV
                     GTGRRSGIFSDEDMRMMAENRGMKSKIERSMFSDAIATMIGAVVGTSNVNTYVESATG
                     IAAGGRTGLTSVVVACCLAASAFFASLVSAVPLAATSPILVIVGILMAASFADVAWQE
                     FEVAAPCFFTAVGMTLCYSISYGIAAGFIVYCLIRIYKGEARAVHPLMWIFTGLFVLN
                     FVVMALI"
BASE COUNT          397 a          550 c          390 g          368 t
ORIGIN      
        1 gccggaacca aacggcggaa gagaaagtgc ctcctcaacc gctctggaaa cagcgggggg
       61 tttgggccag ctggtcgcag cattattcag ataaatgagg ggagatgcag aaaccattct
      121 tctgggaaag tttctgcatg agatctctat aagggtttcg accacaatgt tgaggatcaa
      181 accgccgggt attcctcaca tgccgactgg gaaaagtatc tccgcctatc tgacgatgat
      241 tcggccagag atattaaata attctgtgca aaacaatagc atatacccct cacagattcc
      301 gcccttgcaa agacaggctt tgtcctgacc tccctgctct gctttgccct ctacctgttc
      361 cttgccctca caacgctgtc tctctcattc ctgccggcaa cccccgtaac atatgctgtt
      421 ccggtcatct gtctcttcct tcccctggca ggcggtctgc tctatctccg gtacgggaga
      481 ccggaacatc ccaaacccct gtatctgttc atcgcagttc tgggagtggg aattctcacc
      541 acacttatcc agggactcag cgcagttccg gctctcctcc ttctcggcat tctttcgcct
      601 gcggttctct gtctcgtcct gattactctc ccggccaccc cgttcctgca aacactcaga
      661 cgcatctccc ttcttctggc aggagtgaac ctggtgcttg gcctctgtat cccggcatac
      721 atggatgccg cggtcgctgc ggccggagga atgaccacgg gagaggccgt gatcgttatc
      781 agcatcgtgc tgatcctttc cgttctcact ccggtgtacg ggatcatcta tcttttcggc
      841 ggggttcgtg ccctcagatc agtgccatca ccacaaagtt cagcacaaac agacccgtaa
      901 agatccacat caggggatgc acggcccgtg cttccccttt gtagatccgg atcagacagt
      961 acacaataaa cccggcagca atgccgtagg agatactata gcacagcgtc ataccgaccg
     1021 ctgtaaaaaa gcacggagcc gccacctcaa actcctgcca ggccacatcc gcaaaggatg
     1081 ccgccattaa aatacccaca atcaccagaa tcggtgacgt tgccgccagc ggaaccgcac
     1141 tcaccagact tgcaaaaaac gcactcgccg ccaaacagca ggcgaccacc acgctcgtca
     1201 gaccggttcg tccgccggca gcgataccgg ttgcactctc cacatacgta ttcacattcg
     1261 acgttcccac caccgctccg atcatcgttg cgattgcatc cgagaacatc gaccgctcga
     1321 tcttgctttt catcccccgg ttctcggcca tcatccgcat atcctcatca gaaaagatcc
     1381 cgcttctccg gccggttccg accagtgtgc cgatggtatc gaacatatcg cacagtccga
     1441 acgcgagaat tgtcaccagc accagcggca gccgcatcgg gtccgagaac agcgaaacca
     1501 gtcctgcatc cgtaaagatg acaccaaacg ttgacggaag agcagcccac gcctcggaga
     1561 tgctgatcac atttgccgat tctgttactc cgagcggaat gcccaccaca gcagccgctg
     1621 cgattccgat caggagggca cctttcacct gtttgaccaa aagcagcagt gtcaccgcaa
     1681 atccgatcag aaacacccag ataac
//
LOCUS       DALTZZ010000260         1683 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_44523,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000260 DALTZZ010000000
VERSION     DALTZZ010000260.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44523"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(351..674)
                     /locus_tag="O0X12_05015"
     CDS             complement(351..674)
                     /locus_tag="O0X12_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJK53546.1"
                     /translation="MTFHPTNNPVFWKQTADELLASSRFEKAHAAYLQLCELTPNDAE
                     AWRGMAQALTGLERYSDAIAAYERTLVLLPDDTVALNGLAGCYEKLGDFEKMTACRIR
                     LSELD"
     gene            complement(714..968)
                     /locus_tag="O0X12_05020"
     CDS             complement(714..968)
                     /locus_tag="O0X12_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53547.1"
                     /translation="MYLVIVRGRDNPREVLELANGIEAVHYSDTEGWLILDVTLIDEA
                     THAKKPCKLSYHLGNGEIWYDNGDEVKINNVTGIFTTVQA"
     gene            complement(1037..1109)
                     /locus_tag="O0X12_05025"
     tRNA            complement(1037..1109)
                     /locus_tag="O0X12_05025"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1073..1075),aa:Glu,seq:ttc)
     gene            complement(1191..>1683)
                     /locus_tag="O0X12_05030"
     CDS             complement(1191..>1683)
                     /locus_tag="O0X12_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HJK53548.1"
                     /translation="YEKGLTAVTVCVRRNTPDSLPPNIKSLNYLNNILGKIEANYKGG
                     DEAIFLDRTGKLAEGSGDNIYLVKGGKLYTPPTINNLKGITRHVLLELLDKLEIPYEI
                     CELGLFDLYTADEVMVTGTAAEICAITKIDGRVIGDGKPGAVYRRLIPAFQEVTQNEG
                     DAY"
BASE COUNT          439 a          383 c          404 g          457 t
ORIGIN      
        1 ccgtacaatg cctgcgtgcc cagtacattc gctgcctcaa agaagatttt ccacatataa
       61 tgcgaatgta acctctagtc tgtagattcc ggaaaggggt catatgttct gggagaacac
      121 gtgacggatt catgtagatc acccagagct cttcccggct tatcctggtt tgggatgatg
      181 agtctgggta aggaagaggg atcgatccta tatccaaaaa ggatctaaaa attttcttta
      241 ctgaaaccgg gtgcaataga cagattcgta cttgcagaat atctggatca tagtccagta
      301 attttctgaa tcggatggtt tacccaccta cgtatcggca taggttctct ttagtccagt
      361 tcactaagcc gtatccggca cgcagtcatc ttctcaaaat caccaagttt ttcataacat
      421 cccgcaagac cgttaagcgc aacagtatca tccggcagaa ggacgagagt acgctcatat
      481 gcggcaatgg catcactgta ccgttcaaga cctgtaagag cctgtgccat accgcgccac
      541 gcctctgcat catttggtgt tagttcgcac aattgaaggt acgctgcgtg agccttttca
      601 aaacgggaag atgccaatag ttcatcagcg gtctgtttcc agaacaccgg gttgttcgtg
      661 ggatgaaatg tcataccagt acttgggtgt cccctacaaa aaagagatgg atattatgcc
      721 tgaacagtgg tgaagatacc agtcacgttg ttgatcttaa cctcgtcacc attgtcgtac
      781 cagatctcac cgttgcccag gtggtaactt aatttacacg gttttttggc atgtgttgct
      841 tcgtcaatga gggtcacgtc caagataagc cagccctcag tatcggagta gtgaacagct
      901 tcaataccgt ttgcgagctc taaaacctct ctggggttat cccgtcctcg tacgattaca
      961 agatacatac tgtgatcttt tgtgatccaa caaagataaa cattcagaaa caggtaagat
     1021 aaaacaaaaa ggttagtgct gatatcggga ttcgaacccg agtcgctggc gtgaaaggcc
     1081 agcatgattg gcctctacac tatatcagcc caatcactgc tgcatcgcag tattgcctat
     1141 agaggttgtc gtgaaaaaat aaaaagatta tgatatcaat ctgatgaggg ttagtatgcg
     1201 tcgccttcgt tctgggtgac ttcttggaac gcagggatca gtctgcggta cactgcgcca
     1261 ggttttccat cgccaatcac tctgccatca attttggtga ttgcacagat ttctgcggct
     1321 gttccggtaa ccataacctc gtcagcagtg tacagatcaa acagaccaag ttcacagatt
     1381 tcatagggaa tctcaagttt gtccagaagt tcaagcagga catggcgcgt aattcccttg
     1441 aggttgttga tggtcggcgg ggtgtagagt ttaccgcctt tgaccagata gatgttgtca
     1501 ccggagccct cagcaagttt tccggtacgg tcaaggaaga tagcttcgtc accgcctttg
     1561 tagtttgcct cgatcttacc aaggatgttg ttgagatagt tcagtgattt gatgttgggc
     1621 gggagagagt caggagtgtt gcggcggaca caaacggtta ctgcagtcag acctttttca
     1681 tac
//
LOCUS       DALTZZ010000261         1851 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_44763,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000261 DALTZZ010000000
VERSION     DALTZZ010000261.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44763"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(64..774)
                     /locus_tag="O0X12_05035"
     CDS             complement(64..774)
                     /locus_tag="O0X12_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53549.1"
                     /translation="MAVQTKSNAGDKPRQKKDINWTQIAVVAFCVLLVAMCIISFSNL
                     SNIFNQNGTTTSGTVTSGDLVYVDYTMYIGDVPMVTSSIDVYNESPADRLPAVTSMLG
                     LIAGQQVDVVNGTPLLMNSSYEYPFRMLNQEYNQIATGVIGLGAGQSRTVESTGSYFQ
                     TTYTRDVAEEMGIDYDNWTVGTMGLLNFPVVNETSNTTTMAIRPALVTNKTADEMVLQ
                     YGYDTIEMTVVQAYRTSA"
     gene            927..1412
                     /locus_tag="O0X12_05040"
     CDS             927..1412
                     /locus_tag="O0X12_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HJK53550.1"
                     /translation="MSIRPYQFSFTSLGSELDSLFAEMEARATALMNQSGASTLPAAV
                     KSGNYSVFGNDFHVDLCENENEIIVVTDLPGVEKENISVKLLNPETLMIKTEQQIQTE
                     EGTEGTYHLRERRLGSMQRNIHLPAPVKAEGAKASFKNGVMEITLQKEVAEEGTTIAI
                     E"
     gene            1533..>1851
                     /locus_tag="O0X12_05045"
     CDS             1533..>1851
                     /locus_tag="O0X12_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HJK53551.1"
                     /translation="MEKKQVRDYMTHDVISVDAGETIGDVIRLIRTTHHDGFPVVRQG
                     KVVGYISARDIIGEHPLTTVDQRMSRHPIKATSDLTITEVARRIFRSGIQKLPVVDAE
                     NTLI"
BASE COUNT          478 a          422 c          495 g          456 t
ORIGIN      
        1 aaaaccgcga tgcgatcaaa aaatcataaa attatttttt ctgaaatttg taaaaagaaa
       61 gtgttaggcg gatgtccgat acgcctgaac aacggtcatc tcaatagtat cgtatccgta
      121 ctgaagaacc atctcatcag ctgttttgtt ggttacaaga gcaggacgga ttgccattgt
      181 tgtggtgttg ctggtctcgt ttacaaccgg gaagttcaga agacccatcg tgccgacggt
      241 ccagttgtcg tagtcaatgc ccatctcttc tgctacatct ctggtgtagg ttgtctggaa
      301 gtaggaaccg gtggactcaa cggtccggga ctgaccagct ccaagaccaa taacaccggt
      361 ggcaatctgg ttgtactcct ggttcagcat ccggaacgga tactcatagg atgagttcat
      421 cagcagcggg gtaccgttga ccacgtccac ctgctggcca gcaataaggc cgagcatcga
      481 ggtaactgcc gggagtctat cagcggggga ttcgttgtag acatcgatgg aggaggtgac
      541 cataggaacg tcaccgatgt acattgtata gtcaacgtat acgaggtcgc cggatgtgac
      601 cgtgcctgac gtggtggttc cattctggtt gaagatattg ctaaggttgc tgaaggagat
      661 gatacacatc gccacaagaa gaacacaaaa tgcgacaaca gcaatctgtg tccagttaat
      721 atcttttttc tgacggggtt tgtcacccgc gttactcttc gtctgtactg ccatttgtct
      781 atagtatggg aaggtatgcc tcataaagta gttgctgatc tgatttccct catttagaaa
      841 tatttaaata aaattatgac ttactaatag taagtcacaa tgaaccgccc agtgcgagtg
      901 accaatacta tacgaggtaa aatatcatgt caattcggcc atatcagttc agtttcacat
      961 cccttggcag tgagcttgac tcactctttg ccgagatgga ggcacgtgca accgccctta
     1021 tgaaccagtc cggtgcctcg acactcccgg ccgcagtaaa gagcggtaac tactcggtgt
     1081 ttgggaatga cttccatgtg gatctctgcg agaatgaaaa tgaaatcatt gttgtgaccg
     1141 atctgccggg agtggagaag gagaatatct ctgtcaaact tctgaatccg gagacgctga
     1201 tgattaaaac cgagcagcag attcagacag aggagggtac ggaaggaacc tatcatcttc
     1261 gcgaacggcg gcttggcagt atgcagagaa atattcatct gcctgcccca gtcaaggcag
     1321 agggtgcaaa ggcaagcttc aagaacggcg taatggagat taccctgcaa aaggaggtag
     1381 cagaggaggg cactactatc gcgattgagt gagatttgtt gtaaccagcc gttctgcagg
     1441 gagtctgccc gatcccccga tacacacccc aatatccccc ttttttgttc gatgcattga
     1501 tgctgtttca ccacgaatta ctatcttaag caatggagaa gaagcaggtc cgggactata
     1561 tgacgcacga cgttatcagc gttgatgcgg gcgagaccat tggtgatgtg atccggctga
     1621 tccgtacgac ccaccacgac gggtttccgg tggtgcggca ggggaaagtg gtcgggtata
     1681 tttccgcacg ggatattatc ggggaacatc cgctgacgac ggtggatcag cgtatgtcgc
     1741 gccatccgat taaggcgacg tcggatctga cgatcactga ggttgcccgg aggatcttcc
     1801 gttccggaat ccagaagctg ccggtggttg atgcggagaa tacgcttatc g
//
LOCUS       DALTZZ010000262         5837 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_44862,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000262 DALTZZ010000000
VERSION     DALTZZ010000262.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5837)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5837)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5837
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44862"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(220..2145)
                     /locus_tag="O0X12_05050"
     CDS             complement(220..2145)
                     /locus_tag="O0X12_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HJK53552.1"
                     /translation="MTSTPPSLKDRKKMRLAVLEAEIFSARMQVKAEKLYLYALISGC
                     LFGTVLYLFLLYLSLRMVPPSLFLNAPEFCVIFGVTLLVPAFAAGYGVFAGGLYLPKV
                     LCKLTGTRIDPSLFHAVTYLYALHQSEPNLYAVVESLAKYAASYGEAAREFRQVISDC
                     RNCSLDFYSALGRLSQTTPSEKFRSFLTGLSSSYRTLGSATEYLRMKTDELREEQRLS
                     QKMYLNTLGVIAEIYITVFVAGPLFLVIVVMVMGVISSVNPLVLALIIYVMLPFGTAL
                     FLLILSVISDTHEETTTEIMHAEQSEEIRKQVFSSVRITIPQVSEAPAFEKLARRDAR
                     EKYLRFFHAPVSYMKFHPPAVFACSVPAALLLMAVLYLLFVPVPLTPDGFLSWIAAAE
                     NVVIAGVLIALVPFAVFYEIQTRYRRRVEGAVPDFVDQLVSAVRHNLTFSQAIDLIAL
                     EGRSDMLEEIRMVRRDILWGSRTSDAVRRFRENVPVGVIDRMSVLLMQAEHFTDNLTQ
                     VLQIIAEDSKSLVMRKNERRGEMQLYVIVVYLAFFVFVFVQSIMAVMFLPMLLQSGSG
                     LLVIGSGVTNTVTGWSPADIYNCLIYHSVLIHGFCSGLVAGMMGEGSVCAGIKHSCVM
                     IAVAVVVFTFVKFFLLT"
     gene            complement(2142..3770)
                     /locus_tag="O0X12_05055"
     CDS             complement(2142..3770)
                     /locus_tag="O0X12_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HJK53553.1"
                     /translation="MFQKKRKRIPVPEYDFVRDSPLVTPEYDPSLTLVEAYWTRPGLA
                     LVVIVLTPANEYQYLVFEPSLTPFEKTVLDRLFPGVRDLLIRTDVAMDEESRTKALYD
                     AMDLYLERFSIDLTPDAVQKIRYYLKRNYLGWGILDPLQQDVEIEDISCDGYSTPIFL
                     YHRRYRNIRTSIRFCTQEELDSMVVRFAQSAGKQISLASPIVDTALADGSRAQLTYGK
                     AVTSCGSSFTIRKFREIPFSPIDLILNNTFTVAEMVYFWMAVEYSQSILFIGGTASGK
                     TTSLNAVAQFIPHLSKVITIEDTREITLSHENWLSSVVPDVANPSDSRGRIEMFDLLK
                     AAMRQRPEYILVGEVRGVEAQTLFQAMNTGHTTFSTLHAGSVDSAIHRLENEPLNVPK
                     AMIASLNIVSSQIRLYREGKQIRRCSEIVEIMGISETKDVVVNTVFKYRPDTDSVIYS
                     ARSEIYNCIAEISGNDVNWVVREQQRRAVVLTAMVEQQIRDYRDVSEILWRYAAAPEL
                     VLSSLDDLTVLLEHGSVTPRPRRNMKADVVPPSV"
     gene            4058..4831
                     /locus_tag="O0X12_05060"
     CDS             4058..4831
                     /locus_tag="O0X12_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53554.1"
                     /translation="MPDTGERRVLHATRIPSFDKVIGGGVIPGSTILVLAEPGSGGRE
                     FIQTSLVNYCSDSVNRVSPPEGVQHPAEIHCISLACSRDVFEQQMEDLWNTKRDSQFQ
                     KMMERVHYTDLGEEIGGDLARLFERINAVPDGSVLFIDSLTVLLPYCTRTPDTWVELV
                     TMMRRVTRSAKKRRTTILFHLTSGILTRGQENELSDTVDVVLNMFWQKNVTVKRQRQM
                     YLLKLDGRMPRIDQRDMVIFNVNITPGTGFEITNMRRVA"
     gene            complement(4870..>5837)
                     /locus_tag="O0X12_05065"
     CDS             complement(4870..>5837)
                     /locus_tag="O0X12_05065"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HJK53555.1"
                     /translation="HLVIDPDTAASLLARMNEGAKAHPESRVVTAGVSLSKGSSTLAN
                     SHGVWYERRMTGVSLGLDAIADGSTSYEYDVSPFLSRIDPEKMGEQTAFLAAASRNGV
                     AVPTGRQDVVLSEHVVDSLILELFTESINGRNVLTGKSVFSGKLGETVAAPSFSIADV
                     PMDPKGNSMRRFDTEGTPSSRCSILENGVLSSFLYDCKTAAQAGTASTGHALRGGNGA
                     TYISPHCLQVSGTVADVTKEPCLFVREVIGAHTANPLTGEFSVEVANAFLMEDGEFVQ
                     PVKKAMIAGNVFEILQQVDGISAETKVFDGAIVPKMRIPSMQVIG"
BASE COUNT         1626 a         1529 c         1339 g         1343 t
ORIGIN      
        1 attgtctcgt actttaacct cgcccctatc ggtgatcttt ccattgccca ggttattgtc
       61 tgtgccgttg gcggaatact tggcgcagca ctgctgatca tcctttcctt ctttggaatc
      121 atcatctaac aatctctttt tcatctcttt cggcagccta aaggcaagga acggatcagg
      181 ttccggacgg aaaacagaac cagagacgaa cacgatcact tacgtcagaa ggaaaaattt
      241 tacaaacgta aacaccacca ctgcaaccgc tatcatcaca cacgagtgtt tgattcctgc
      301 acagaccgag ccttcaccca tcatccctgc aacaagtccg gaacagaacc cgtgaatgag
      361 tactgaatga taaatcagac aattatagat gtcagctggg gaccatccgg ttacagtatt
      421 cgtcactcca gaacctatta caagaagacc gcttccgctt tgcagcagca tcggcaaaaa
      481 catcaccgcc ataatgctct gcacaaagac aaacacaaaa aatgccagat acaccacgat
      541 cacatacaac tgcatctctc cccggcgttc gtttttcctc atcaccagac tctttgagtc
      601 ctcggcaatg atctggagaa cctgtgttaa attgtctgta aaatgttcag cctgcatcag
      661 cagaacactc atccgatcga tcactcccac cggcacattc tcacgaaacc tccttaccgc
      721 atccgatgtt ctgctgcccc acagaatatc acggcgaacc atccgtatct cctccagcat
      781 atcactccgt ccctcaagcg caatcagatc gattgcctgt gaaaacgtca gattatgccg
      841 gacagcagag accagctgat ctacaaaatc gggaacagca ccttctactc ttcgccggta
      901 ccgggtttgt atctcataaa ataccgcaaa cggtacgagt gcgataagga ctcccgcaat
      961 cacaacattc tccgctgccg caatccacga aagaaaccca tcaggtgtta gtggaacagg
     1021 tacaaacagg agatacagga cagccatcag caaaagtgct gcaggaacac tgcatgcaaa
     1081 caccgccggt ggatgaaact tcatataaga aacaggggcg tggaaaaacc gcagatactt
     1141 ttcccgtgcg tcacgcctgg caagtttctc aaacgccgga gcctccgaga cctgcggaat
     1201 cgttatgcga acagaagaga acacctgttt ccttatctct tcactctgtt ctgcatgcat
     1261 gatctctgtt gtggtttcct cgtgtgtatc tgagatgact gaaagaatca acaggaataa
     1321 cgcagtccca aacggtaaca tcacataaat gatcagagca aggaccagcg ggtttacact
     1381 tgagataact cccatcacca tgacaacgat tacaagaaag agggggcctg caacaaaaac
     1441 tgtgatgtag atctctgcaa taactcccag tgtgttcagg tacatcttct gggagagccg
     1501 ctgctcttcc cgcagttcat ccgttttcat ccgaagatac tcggttgcgg aaccgagtgt
     1561 acggtatgat gacgacaaac ccgttagaaa ggaccggaac ttctcagacg gtgtggtctg
     1621 ggaaagtctc ccaagcgctg agtaaaaatc cagggaacag ttccggcagt cactgatgac
     1681 ctgccggaac tcgcgtgccg cctcaccata cgaggcggcg tattttgcaa gtgattccac
     1741 cacagcatac agattcggct cggactgatg cagtgcataa agatacgtta ccgcatgaaa
     1801 cagcgagggg tcaatacgtg tccccgtgag tttacacaga actttgggca gatacaagcc
     1861 cccagcaaac accccgtatc ccgccgcaaa cgcaggcacg agaagtgtga caccgaaaat
     1921 cacacaaaat tccggagcgt ttaggaataa tgacggcgga accatccgca gggagagata
     1981 taaaagaaaa agatacagaa cggttccgaa caaacatccc gaaatcaatg catacagata
     2041 cagcttttct gcttttacct gcattcgtgc cgaaaagatc tctgcttcaa gaactgccag
     2101 acgcatcttc tttcgatctt tcagagaggg aggggtactc gtcatacgga tgggggaaca
     2161 acatcggctt tcatattccg gcgcggacgc ggtgtcacac tgccgtgttc aagaaggact
     2221 gtgagatcat ccaatgaaga aagaaccagc tccggtgctg ctgcatacct ccagagaatc
     2281 tccgacacat cgcggtagtc gcggatctgc tgttcaacca tcgccgtgag aacaactgct
     2341 ctgcgctgct gctctcggac aacccagttc acatcattcc cggagatctc agcgatgcag
     2401 ttatagattt cagaacgtgc ggagtagatc acactgtctg tatccggccg gtatttgaac
     2461 actgtattta ctaccacatc cttcgtctct gaaatcccca taatttccac aatctcactg
     2521 cagcggcgaa tctgctttcc ttcacggtac agccggatct gggaggaaac aatgttcagt
     2581 gatgcgatca ttgctttcgg aacattcagc ggttcatttt ccagccggtg gatcgcagag
     2641 tccaccgaac ctgcatgaag cgttgaaaac gtggtgtgtc ctgtgttcat tgcctgaaac
     2701 agtgtctgtg cttcaacccc acggacctct cccaccagga tatactcagg acgctgacgc
     2761 atggcagctt tcagcaggtc aaacatctcg atccttccac ggctatcgga ggggtttgca
     2821 acgtcaggta caacacttga cagccagttt tcatggctca gagtgatctc tcgtgtgtcc
     2881 tcaatggtga tcactttgga aagatgcgga ataaactgcg caaccgcatt cagggaggtt
     2941 gtctttcctg atgctgttcc gccgataaag agaattgact gactgtactc cactgccatc
     3001 cagaagtaca ccatctcagc aaccgtgaag gtgttattca aaataagatc aatgggcgaa
     3061 aacgggattt cccgaaactt ccggatcgta aatgatgagc cgcagcttgt taccgccttc
     3121 ccatacgtca gctgggcacg tgatccgtcc gctaaggctg tatctacgat cggtgatgca
     3181 agggatatct gttttcctgc cgactgtgca aaccgaacca ccattgaatc aagctcttcc
     3241 tgggtacaaa accggatgga ggttctgata ttccggtacc tgcggtgata caaaaatatc
     3301 ggtgtactat acccgtcaca ggagatatcc tcaatctcca catcctgctg cagcggatca
     3361 agaatgcccc atcccagata attccgtttc agataatagc ggattttctg cacggcatcc
     3421 ggcgtaagat caattgaaaa ccgttccaga tacagatcca tcgcatcata cagcgctttg
     3481 gtcctgctct cctcatccat tgccacatcc gtacggatca gcagatcacg aactcccgga
     3541 aacaatcggt ccaggacagt tttctcaaac ggggttagcg agggttcaaa aacaagatac
     3601 tggtactcat ttgcaggagt aagcacaatc acaaccagtg caagacctgg acgtgtccag
     3661 tatgcctcca caagggtgag ggacggatca tactcgggcg taacaagcgg gctgtcacgc
     3721 acaaaatcat actctggaac tggaatgcgt ttcctttttt tctgaaacat cctctgtttc
     3781 actccaactc agaaggtgtt gagcagaacc ggaacagtac ctgttaccag tggacaaatt
     3841 ttgtcgtcag tcacaggcct catgcaaacg ctcaggcata tattatcccc ttgggcatga
     3901 taatacttgc cactgataat acaaaaattc ctgaaatcag atgatcagat ctctgttcaa
     3961 ttctattttt aggaaatatc aatgagatct gggaaagaaa caatcatatg atcacggaaa
     4021 attataaatt ctcaaaaaag ggagataggt gtaatctatg ccggatacag gagaacgacg
     4081 tgtgctacac gcaacccgca tcccatcctt tgacaaagtg attgggggag gggtcattcc
     4141 gggatccacc attcttgtcc ttgcagaacc aggttccgga ggaagggagt ttatccagac
     4201 atcactcgtg aactactgca gtgatagcgt gaacagagtg tctcccccgg aaggagtgca
     4261 acatccggct gagatccact gtatctccct tgcatgcagc agagatgtct ttgaacagca
     4321 gatggaagat ctgtggaaca caaagagaga ttcccagttc caaaaaatga tggaacgtgt
     4381 tcattatacc gatcttgggg aagagatagg aggtgatctt gccagactat ttgagagaat
     4441 aaacgcagtg cctgatggga gtgtactttt tattgattct ctgaccgttc ttcttcctta
     4501 ctgtaccaga acaccggata cctgggtgga acttgttacc atgatgcgca gagttacacg
     4561 gtcagccaaa aaacgaagga cgaccatcct attccacctt acttcgggaa tcctcacccg
     4621 aggacaggaa aatgaactca gtgacaccgt tgacgtggtc ctgaatatgt tctggcagaa
     4681 aaatgttaca gtcaaacgtc agagacagat gtacctgctg aagctcgatg ggagaatgcc
     4741 gagaattgat cagcgggaca tggtgatctt caacgtgaac ataaccccag gtacgggatt
     4801 tgagatcaca aacatgaggc gggtggcata ggtacacagc ctgatgtaca aaccgcaaaa
     4861 aaagtgagat tatccgataa cctgcatgga cggtatgcgc atcttgggaa caattgcgcc
     4921 gtcaaaaacc ttggtctccg cactgatacc gtcaacctgc tgtaaaatct caaacacatt
     4981 tcctgcaatc atcgcttttt tcaccggctg cacaaactct ccgtcctcca tcaggaaagc
     5041 atttgcaact tccaccgaaa actctccggt cagaggattt gccgtgtgag caccgataac
     5101 ctcgcggaca aagagacacg gttctttggt cacatcagct actgtgcctg aaacctgcag
     5161 acagtgcgga gagatgtacg ttgccccgtt tccgccgcga agtgcatgac cagtggatgc
     5221 agtacccgcc tgggcggcag tcttgcagtc gtagaggaag gaggaaagta caccgttttc
     5281 aagaatactg cagcgggatg aaggtgtacc ttcagtatcg aatctccgca tgctgttccc
     5341 tttgggatcc atcgggacat cagcaatgga aaaagagggg gccgcaacgg tctcaccaag
     5401 ctttccggaa aaaacagatt ttccggtgag aacatttctt ccgttgatac tttccgtaaa
     5461 cagttccaga atcagcgagt caaccacatg ttccgacaga acaacatcct gccgtccggt
     5521 cggaaccgct acaccattcc gggatgcagc cgccaaaaat gctgtttgct cacccatctt
     5581 ttcgggatca atgcgggaaa ggaacggcga gacatcatac tcgtaactgg ttgagccatc
     5641 agcaatcgca tctagtccaa gggacactcc ggtcatacgc cgctcatacc agacaccgtg
     5701 actgtttgca agcgttgagg aacctttcga gagggaaaca ccggcagtaa ccacacgcga
     5761 ttcaggatgt gctttcgcac cttcattcat acgtgcaaga agcgacgctg cggtatcagg
     5821 atcgattaca agatgag
//
LOCUS       DALTZZ010000263         1923 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_44901,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000263 DALTZZ010000000
VERSION     DALTZZ010000263.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44901"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="O0X12_05070"
     CDS             <1..218
                     /locus_tag="O0X12_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HJK53556.1"
                     /translation="FIYLIKYSSLAYVVTVIELTGAAKIIASKYFAYFEAFAAVGVFY
                     LVIVTIATIAINRLEKKYAIPGTGAGK"
     gene            261..512
                     /locus_tag="O0X12_05075"
     CDS             261..512
                     /locus_tag="O0X12_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HJK53557.1"
                     /translation="MSFSLDVIDKLSALITAAFGLVAALAWNSAIQQIFKEIFGEQSS
                     IPAMLGYAVFVTVIAVIFTLWIGFVSNKAKEKIVPEKKE"
     gene            597..1540
                     /locus_tag="O0X12_05080"
                     /pseudo
     CDS             597..1540
                     /locus_tag="O0X12_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833999.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
     gene            1641..>1923
                     /locus_tag="O0X12_05085"
     CDS             1641..>1923
                     /locus_tag="O0X12_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53558.1"
                     /translation="MRVYCAQIAQVWNDPDAAFSRAHDAVLRAVEADADLVMFSEQFA
                     TGWRPSMGSVQTGFSGAEVKERWCSLARECGVAVAGSYAREVPGALPQNV"
BASE COUNT          350 a          400 c          556 g          617 t
ORIGIN      
        1 agtttatcta tctgatcaaa tactcctccc ttgcctatgt ggtgacggtg attgaactga
       61 ccggtgcggc caagattatt gcaagtaagt actttgccta ctttgaggcg tttgcagcag
      121 tcggagtgtt ttatctggtg attgtgacca ttgcaaccat tgcgatcaac cgtcttgaga
      181 aaaagtacgc gattcccggt accggtgccg ggaagtgagg tgttacggtt taagtatttt
      241 tgatccctaa tagacacagc atgtcattta gtcttgatgt gatcgataag ctttctgctc
      301 ttattactgc tgcatttggt cttgttgcag cgcttgcgtg gaactcggca atccagcaga
      361 tattcaagga gatctttggt gagcagagca gtattcctgc gatgctcggg tatgcggttt
      421 ttgtaacggt cattgctgtt atcttcactc tttggattgg gttcgtttcc aacaaggcaa
      481 aggaaaagat tgtgcctgag aaaaaggagt gatccttttc tccacttttt gaacaatatt
      541 tattatcggg tattttcata cgtgtataca gcgcgtaaca acgcgttgtt gtttgaatgg
      601 agcttcttta cgctattgtt gtaatcatag ggctgttcat ttttgagtcg atcgcaagta
      661 ttgataatgc gatcatcaat gctgatattt tagccactat gaaagaaaag gcccggcgct
      721 ggttccttgt atggggtatc cttctgtcgg tgtttcttgt ccgcggtgtt ttgccgtggc
      781 tgattttgtg ggcggcgaat ccgtctctcg gaccgattgg tgcgctcacg gtaacgttta
      841 ccgatgatga tctggcgatg tctgcaatgg agagtactgc tccctatctg ctgatgtttg
      901 gaggtgtctt tatgctgttc ctgttctgtc actggctgtt tgccgagaag aaggactgca
      961 ttatgccggg agagcggttc ttaacaaaac aggctccgtg gttctacgcg gttgtcgcgg
     1021 ttctcctgct gatacttgtc tgggcaacac tggagtggga tccggttctt gcatttgctg
     1081 cggttgcggg ttcaaccctg ttcttccttg tgcagggttt ccgtttgtcg gcggatatga
     1141 agtcggcgga gctaggtcac agtgagaagt ctgatgcggc gaagctgatg cttcttctgg
     1201 ttatcgacag tacgttttcc attgacggtg ttatcggtgc gtttgcatta cgttctcggt
     1261 tccgctgatc tttatcggat gcggtcttgg tgcgattgtt gtccgggagc tgacgattaa
     1321 gtacatcgat acgatcaagc gctatatcta tctgaagaat ggtgcgatgt actcggtttt
     1381 gatgctgagt ctgatcatgg ttgcggaagg ttttggtctg catgtgccga cctggcttgc
     1441 accggttgcg atggtcgtga ttgtgtcggt gttcctgtat atttcggtgc ttcgcatccg
     1501 ccatggaaag ggtccggtct gtccggcacc cctcaagtaa ttttttgaac gacctgggtc
     1561 gcagtctctc ttttgcggga ctgcgatgat atccccggtt aatcacaatc ttttttttgc
     1621 tgcacgcgga agtgtcaggt atgcgagtgt actgtgctca gattgcccag gtgtggaatg
     1681 atccggatgc tgcattttcc cgggctcatg atgctgtttt gcgtgctgtg gaggctgatg
     1741 cggatctggt gatgttttcc gagcagtttg caaccggctg gcgtccttct atgggttctg
     1801 tgcagaccgg gttttccggt gccgaggtga aggagcggtg gtgttctctt gccagggagt
     1861 gcggggttgc ggttgcgggg tcgtatgccc gcgaggttcc gggagctctt ccgcagaatg
     1921 tga
//
LOCUS       DALTZZ010000264         4132 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_45048,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000264 DALTZZ010000000
VERSION     DALTZZ010000264.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45048"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="O0X12_05090"
     CDS             <1..404
                     /locus_tag="O0X12_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methanogenesis marker 9 domain-containing
                     protein"
                     /protein_id="HJK53559.1"
                     /translation="LQLLDRYIRATAEENGWYNAPKQLCRGGDLRSLTFCCMPVKQCP
                     LLPTLDTIGMSRQEYLALKQDLTKGTEIAKGSHTCFGSLAWCCKSSTPCMFRDMTLKS
                     LPLAKNEYMAMKRELADGIMEKIFADVPTDC"
     gene            385..1215
                     /locus_tag="O0X12_05095"
     CDS             385..1215
                     /locus_tag="O0X12_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HJK53560.1"
                     /translation="MSRPTVSPASLAEQAQFAMLLEVTARGKPGNIDRCHDYEDTRLD
                     HFLSSAVLAKPIFSAIEAGNLSIGEAMRDAVARTNMHRGGNTHFGAFLLLLPLIAGKG
                     IAGATELVKKTTVTDAVLFYEAFGLTQVRVRTEDPMDVNDPASIQRLKDEQITMYSVM
                     EYSAPHDMVAREWTNGFALTRRAADLLFAQKGGVHAIPAMFLTLMTEYPDTFIAKKFD
                     EATAEEVMYKARDVRDGRQTLAAFDDECIKAGINPGSLADICIAGIFTALLEGWQWDC
                     "
     gene            1203..1763
                     /locus_tag="O0X12_05100"
     CDS             1203..1763
                     /locus_tag="O0X12_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HJK53561.1"
                     /translation="MGLLTEGITEVIAVTKDNAAPMGIIVKPGMSPRMILFKGSKTVE
                     NILTYGWVTANFVSDSYLYPQYAFSDVEPGDLAEVFVGGIMMQRLLSADAWMAFRTTV
                     LHETEETYYIDLLPVASEYVREDPHPINRGFNSVIDATVHATRYVHSKDEHLRELIEY
                     HLSVVTKCGGPREREAAQLLREICGL"
     gene            complement(1774..2256)
                     /locus_tag="O0X12_05105"
     CDS             complement(1774..2256)
                     /locus_tag="O0X12_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HJK53562.1"
                     /translation="MPENQYSQILFSAGCDAGVVRHCEVVALVASRFSGDSVDDDLVR
                     AGSMMHDIGRSVTHDISHAQTGARICSEHGEPEELTQIVLRHTGAGLDADECTLLGLQ
                     PIDCVPQTLEEKIVAHADNLVKGSKVISIEERMMRIPDLSTRSKKRIWRLAMEVELLG
                     "
     gene            complement(2249..2770)
                     /locus_tag="O0X12_05110"
     CDS             complement(2249..2770)
                     /locus_tag="O0X12_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HJK53563.1"
                     /translation="MTAITEEQLQNPAIYQYLLKLVGDEGIELLRRCPNEELSDEDIA
                     AKTEINLNSVRHTLYNLYEHRLAEYRRIKNNETGWLTYLWVMRMDNLNLVLRSEMETV
                     VGKLSARLRYDEANDFYQCKNCGLMTTFNNACTTNFACEQCGEMLVHFDDELLVSALK
                     SRVEKMREALSDA"
     gene            complement(2767..3843)
                     /locus_tag="O0X12_05115"
     CDS             complement(2767..3843)
                     /locus_tag="O0X12_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HJK53564.1"
                     /translation="MSPSVLVAGYTTRHVAKSAAQQGYDVYAVDHFCDQDLLWCTKDV
                     IAFDELDELPFAIEEMLGRYEIDYVITTSGAELLDVPNRMGTDPTVAARFMDKGKTQG
                     FFESLGVPVPRRLSDGEYPAMMKTLSGAGGWRNAVVRNDEERACWEEFVEHEPFLMQE
                     VISGIPASVSCVGTGSSAKAIVANEQILRGGESCAYAFSGSVTPCVHPMAKRMMDTAE
                     KIVAASGCVGSVGVDFVLTETEAYAIEVNPRFQGTVETVEAATGLSLFKLHMDACQGI
                     LPEIVPQPRQFCVRKILAAPEPLALNADMRTLAGTITDIPHPGTMFEEGEVMFSVLGC
                     GASRAEAFTSLDKHITDAVQHIKT"
     gene            complement(3840..>4132)
                     /locus_tag="O0X12_05120"
     CDS             complement(3840..>4132)
                     /locus_tag="O0X12_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832831.1"
                     /note="catalyzes the S-adenosyl-methionine-dependent
                     2'-O-ribose methylation of C56 in tRNA transcripts;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HJK53565.1"
                     /translation="YGLRLQDVISQIRTEEKILVVVGAEKVPGDMYELADYNVAVANQ
                     PHSEISALALFLDHLYEGEGRELSFPDGELEVLPTAVGKTTVKHEEHHGDEA"
BASE COUNT         1077 a         1184 c          936 g          935 t
ORIGIN      
        1 cccttcagct tctcgatcgt tacatccgtg caaccgctga ggaaaacggg tggtacaacg
       61 caccgaaaca gctgtgccgc ggcggcgatc tgcggtcact caccttctgc tgtatgccgg
      121 tcaaacagtg cccactttta ccaaccctcg atacgattgg catgagccgc caagagtacc
      181 tcgcactcaa acaggactta accaaaggaa cagagattgc aaaaggcagt cacacctgtt
      241 tcggcagcct tgcatggtgc tgcaaatcat caaccccctg catgtttcgt gacatgacgc
      301 tcaaatccct gccgcttgca aagaacgaat acatggcaat gaagcgagaa ctcgcagacg
      361 gaatcatgga gaagatcttt gccgatgtcc cgaccgactg ttagccccgc atccctagca
      421 gagcaggcac agtttgccat gctccttgag gttactgccc gaggaaaacc tggtaacatt
      481 gatcgctgcc atgattacga ggatacgcgg ctcgatcatt tcctctcatc tgcagtgctc
      541 gcaaaaccca tcttctccgc tattgaagca ggtaaccttt ccattgggga agccatgcgc
      601 gatgcagtcg ctcgcaccaa catgcaccgt ggtggcaaca cacattttgg cgcatttctc
      661 cttcttctgc cattaatcgc aggaaaaggg atagccggtg ctacagagct ggtgaaaaaa
      721 acgaccgtca ccgatgcagt tctcttctac gaagcgtttg gactcacgca ggtacgggtc
      781 aggaccgaag acccgatgga cgtgaacgat cctgcatcca tacagcggct gaaggatgag
      841 cagataacca tgtacagcgt gatggagtac tccgctccgc acgacatggt cgcacgtgaa
      901 tggacaaacg gttttgccct gacacgcagg gcagcagatc tgctgttcgc gcaaaagggt
      961 ggagtacacg caatccctgc gatgtttctt accctgatga ccgaataccc tgacaccttt
     1021 attgcaaaga agtttgacga agcaaccgca gaggaagtaa tgtacaaggc acgcgatgtc
     1081 cgggatggca gacaaacgct tgccgcattt gacgatgagt gtatcaaagc aggaataaac
     1141 ccaggttctc ttgcagacat ctgcattgca ggtattttta ccgcccttct ggagggatgg
     1201 caatgggatt gttaactgaa ggaatcaccg aggtcatagc cgttaccaaa gacaatgcgg
     1261 caccgatggg aatcatcgta aaacccggaa tgtcaccgag aatgatcctc ttcaagggat
     1321 caaaaacggt cgaaaacatc cttacatacg gctgggtcac ggcaaacttc gtgtcggaca
     1381 gttacctcta cccgcagtat gcgttctcgg acgttgagcc aggcgatctg gcagaggtgt
     1441 ttgtcggcgg aatcatgatg cagcgtctgc tctctgcaga tgcatggatg gcgttccgga
     1501 caactgttct gcatgaaaca gaagagacct attatatcga tctcttacca gtagcatcag
     1561 agtacgtccg ggaagaccca cacccgatca accgtgggtt caatagtgtc attgacgcaa
     1621 cagtacatgc aacgcggtat gttcacagca aagacgaaca tctccgagaa ctcattgagt
     1681 atcatctcag tgtggtgacc aaatgcggcg gaccccgcga gcgtgaggca gcacagcttc
     1741 tccgcgagat ctgcggactg taattccgcg ggattatccc aacagttcaa cttccatggc
     1801 aagccgccat attctctttt ttgaacgtgt agacagatct ggaattctca tcatgcgttc
     1861 ttcaatcgag atcactttgg atcccttcac cagattatct gcgtgggcaa caatcttctc
     1921 ctcaagtgtt tgaggcacac agtctatcgg ctgaagacca agaagagtac actcatcagc
     1981 atcaagccca gcacccgtat gccggagcac aatttgagtc agctcttcag gttcaccatg
     2041 ttcactacag atacgtgcac cggtctgagc atgagaaata tcatgagtca ccgatcttcc
     2101 aatatcatgc atcattgatc ctgcacgcac cagatcatca tcgaccgaat ctccagagaa
     2161 ccgagatgca accaaggcaa caacctcgca atgccggaca acgccagcat cacatcccgc
     2221 agaaaaaaga atctgagaat actggttctc aggcatccga aagagcctcg cgcatcttct
     2281 caacgcggct tttcagagca gaaaccagca gttcgtcatc gaaatgcacc agcatctcgc
     2341 cacactgctc acaggcgaag ttcgtcgtac aggcattgtt aaacgtcgtc atcaggccac
     2401 agtttttgca ctgataaaag tcgtttgcct catcatatct caggcgtgct gaaagctttc
     2461 ccaccacagt ctccatctcc gagcgcagga caagattcag attatccatc cgcatcaccc
     2521 acagataggt cagccaaccc gtctcattat ttttaatccg acgatactcc gccagccggt
     2581 gctcatagag attatacagc gtatggcgca cggagtttaa gttaatctcc gtttttgccg
     2641 caatatcctc gtcactcagc tcctcgttcg gacaacggcg gaggagttca attccctcat
     2701 cgccaacgag ttttagaaga tactgatata ttgccggatt ttgcagctgt tcctcagtga
     2761 tcgccgtcat gtctttatat gttggacggc gtcagtaata tgtttatcca gggatgtgaa
     2821 tgcttcagca cgcgatgctc cgcatccaag aaccgaaaac atcacttccc cttcctcaaa
     2881 catcgtgccc ggatgaggaa tatcggtgat agtccccgca agagttcgca tatccgcatt
     2941 caaagccaaa ggttcaggag ctgcaagaat cttgcgcaca cagaactgac ggggctgcgg
     3001 tacgatctcc ggcaaaatac cttggcaggc atccatgtgc agcttaaaca gacttaggcc
     3061 ggtcgctgct tccaccgttt cgaccgttcc ctgaaaccga ggattcacct caatcgcgta
     3121 agcttcggtt tcggttaaaa caaaatccac accaaccgac cccacacatc cacttgccgc
     3181 aacaatcttt tcggcagtgt ccatcattcg ttttgccatc ggatgaacac agggagtaac
     3241 cgaacctgaa aatgcatagg cacagctctc cccaccacgg agtatctgct catttgccac
     3301 aatcgctttt gcagaacttc cagttccaac acaggagaca ctggccggaa taccggatat
     3361 tacctcctgc atcagaaacg gttcatgctc cacaaattct tcccagcacg cccgctcttc
     3421 atcgttgcgc acgaccgcgt tccgccagcc ccccgcacca gacagtgttt tcatcattgc
     3481 cggatactct ccgtcagaca atctacgggg aaccggaaca ccaagagact caaaaaatcc
     3541 ctgggtcttc cctttatcca taaaccgtgc cgcaacggtt ggatcagttc ccatcctgtt
     3601 cggtacgtca agaagctctg cgcccgacgt tgtgattaca taatcgatct catagcgtcc
     3661 aagcatctcc tcaattgcaa acggaagttc atccagttcg tcaaacgcaa ttacgtcttt
     3721 tgtacaccag agcagatcct gatcacagaa atgatccacc gcatacacat catacccttg
     3781 ctgcgctgca gactttgcca catggcgggt ggtgtaccct gcaaccagaa ccgatggact
     3841 cacgcttcgt ccccgtggtg ctcctcatgc tttacggtag tcttaccaac ggcggttggc
     3901 aaaacctcga gctctccgtc aggaaaagaa agttcccgcc cctcaccctc atagagatga
     3961 tccagaaaca gtgcaagtgc agagatctcc gagtgcggct ggtttgcaac cgccacattg
     4021 taatcagcaa gctcatacat atcgcccggc actttttcag cacccaccac aaccaaaatt
     4081 ttttcctcgg ttcggatttg ggagatcaca tcctgcaacc gcagaccata ca
//
LOCUS       DALTZZ010000265         3028 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_45420,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000265 DALTZZ010000000
VERSION     DALTZZ010000265.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45420"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="O0X12_05125"
     CDS             <1..362
                     /locus_tag="O0X12_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HJK53566.1"
                     /translation="FEGFAQMTEEVSGRDTLICGDDLFVTNAKRLEEGITLEAGNCVL
                     IKPNQIGTLTDTFETIRLAHEYGYETVMSHRSGETTDTTIAHLGTAFNCCFLKCGVVG
                     GERIAKLNELIRIEEHF"
     gene            364..972
                     /gene="rpsB"
                     /locus_tag="O0X12_05130"
     CDS             364..972
                     /gene="rpsB"
                     /locus_tag="O0X12_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HJK53567.1"
                     /translation="MTDNELGIELTEPLVSVEEYLAAGVHIGTQQKDDDMKEFIYRVR
                     SDGLYIIDIRKTDERIKQVAKFLARYEAPKIFVVTSRQYGQYPAQKFADTIGALSHVG
                     RFIPGTLTNPVLPKYVEPSVVVVTDPIGDSQVITEAVQCGIPVIALCDINNQTNNVDL
                     VIPTNNKGRKALSMVYFLLTREVLKQKGIVSSLTFEDFESEF"
     gene            1249..2094
                     /gene="amrB"
                     /locus_tag="O0X12_05135"
     CDS             1249..2094
                     /gene="amrB"
                     /locus_tag="O0X12_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HJK53568.1"
                     /translation="MKIEEYPATGTADAGCRHADLAGMFYPKEAEELKALLDALFSST
                     VTSVTSPCGVLVPHAGYIYSGRCAACAYAKIPETFSGTFVVIGPSHAGCMTATADLIW
                     ETPIGPVPPDRAFVAALSEAGIPNRPDLLRVQENSLEVQMPFIRYRFPQAKVVPVLLG
                     EQSFAEVHRVADAILRAIDAVHSDNVVILASGDGSHYVPASVAERDDLTVLAAAAKLD
                     VQKFYDKLIEIQPSMCGYGCIAVMMLVAKQRGAQEAKVIMYQTSGDATGDNREVVGYA
                     AMEVV"
     gene            2094..2966
                     /gene="mvk"
                     /locus_tag="O0X12_05140"
     CDS             2094..2966
                     /gene="mvk"
                     /locus_tag="O0X12_05140"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HJK53569.1"
                     /translation="MATWSAPGKVFLFGEHAVVYGKPGIAMAIKPRVMVTVRETRYHQ
                     KPNSPYIAECFRLMDVKGSVYVRSQLPSSSGLGSSAAVTVATLCAINDEFGLEKTRAE
                     IADLAHQVEMSAQNGRASATDTYVSTFGGMVLVRGNEKRRLLPPQNFSLVIGNTLVSH
                     STSEMVGKVAELRKNSPDIANPILDSIAAITMRAMYNLDNQKELGNLMNMNHALLDAL
                     GVGHPVLSKLVLAARSAGAHGAKTTGAGGGGCMYAICSKGARNRVAGAIEGCDARAII
                     TTIDTEGARKEKDE"
BASE COUNT          704 a          795 c          793 g          736 t
ORIGIN      
        1 actttgaagg cttcgcacag atgaccgaag aggtctccgg ccgtgacaca ctcatctgcg
       61 gagatgatct ctttgttacc aacgcaaagc gtctggagga aggaatcacc cttgaagcag
      121 gcaactgtgt cttaatcaag cctaaccaga tcggaaccct gaccgatacg tttgagacaa
      181 tccgtcttgc ccatgagtat gggtatgaga ccgtcatgag ccaccggtcc ggtgagacaa
      241 ccgacaccac aattgcacac cttggaactg cattcaactg ctgcttcctc aagtgcggtg
      301 ttgttggcgg tgagcgtatt gctaaactaa atgaacttat aagaattgag gagcatttct
      361 aaaatgaccg acaatgaact tggaattgaa ctgaccgaac cattagtatc cgtggaagag
      421 tatctggcag ccggtgtcca catcggtacc cagcagaaag acgatgacat gaaggaattc
      481 atctaccgcg tccgttccga cggtctgtat atcattgata tcagaaagac cgacgagcgt
      541 atcaagcagg tagcaaagtt ccttgcacgc tacgaagcac cgaagatctt cgttgttacc
      601 tcccgtcagt acggccagta cccggcacag aagtttgctg acaccatcgg cgcactctct
      661 cacgttggcc ggttcatccc cggtacgctc accaacccgg tcctcccgaa gtacgtcgag
      721 ccgtcggttg ttgtggtcac tgacccgatc ggagactcgc aggttatcac cgaggctgtc
      781 cagtgcggta ttccggttat cgccctttgt gatatcaaca accagaccaa caatgtcgac
      841 ctcgtgatcc cgacgaacaa caaaggtcgc aaggccctct ccatggtcta cttcctcctg
      901 acccgtgagg tcttaaagca gaagggcatt gtgtcctccc tgacctttga agactttgag
      961 tctgagttct aaatctactc ataccatctt tttttcacat actctgactc ctgtattcct
     1021 gtttggtctc agatgtaaat tctgttctcc agattttttt gtaatgaggt ctgcatgcgt
     1081 gatgtcttgg actcccgtta cctcctcact gttgtgacgc aatcattaaa gccctgccaa
     1141 accaacacat aaccaccttc acacacccat ccgcaaatat ctcaatagta tctttcgcgg
     1201 attggcggac gaaatccttg gttacggtgt aatacccgtt tgatatccat gaaaatagag
     1261 gaataccctg ctactggtac ggcggatgca ggctgtcggc atgctgacct tgcgggaatg
     1321 ttttacccaa aagaagcaga agaactgaaa gccctgcttg atgcactctt ttcgtcgacg
     1381 gtaacgtctg taacctctcc gtgcggtgtg ttggttccgc acgccggcta catctactcg
     1441 ggaagatgtg ctgcgtgcgc ttatgcgaaa attcccgaga cattctccgg cacatttgtg
     1501 gtgattggtc caagccatgc gggctgtatg accgcaaccg cagatctgat ctgggaaacc
     1561 ccgatcggtc cggtccctcc tgaccgtgcg tttgttgcgg cactgagtga ggctggtatc
     1621 ccgaatcgtc ctgatctttt gagggttcag gaaaattctc ttgaggtgca gatgccgttt
     1681 atccggtacc ggttcccgca ggcaaaagtt gtgccggttc tgctgggaga gcagtctttc
     1741 gcggaggtcc accgcgtggc agatgctatc ttacgggcaa ttgatgcggt acacagtgac
     1801 aatgtcgtca ttcttgcttc cggcgacggt tcacattatg tgcctgcctc agttgccgaa
     1861 agggatgatc tgaccgttct tgccgcagcg gcaaaactcg acgtgcagaa gttctatgat
     1921 aaactcatcg agatacagcc gtccatgtgc ggttacggct gtattgccgt tatgatgctt
     1981 gttgcaaaac agcgtggcgc acaggaagcg aaagttatca tgtatcagac atctggtgat
     2041 gcaaccggtg acaaccgtga ggttgtggga tatgcggcaa tggaggtcgt ctaatggcaa
     2101 catggagtgc accgggcaag gtcttcctct tcggcgagca tgccgtggtg tacggaaaac
     2161 ccggtattgc gatggcaatc aagccacgtg ttatggttac cgttcgggaa acgcgttatc
     2221 accagaagcc caactccccc tacatcgccg agtgtttccg gctgatggat gtaaagggaa
     2281 gtgtgtacgt cagatcgcag cttccaagtt cctccgggct tggttcttcc gcggcggtga
     2341 ccgttgcaac gctgtgcgca atcaacgatg agttcggtct ggaaaagacc cgtgctgaga
     2401 tcgctgatct cgcccaccag gttgagatgt ctgcacagaa cgggcgggcc agtgcgaccg
     2461 atacctatgt ctccacgttt ggcgggatgg tacttgttcg cggcaatgaa aaacgccgtc
     2521 tccttccccc gcagaacttc tcgcttgtta tcggaaacac ccttgtctcg cacagcacct
     2581 cggagatggt cggaaaggtt gccgagcttc gtaagaactc tccggacatt gccaatccga
     2641 ttctcgactc cattgcggcg attaccatgc gggcgatgta caatctggat aatcagaaag
     2701 agctcggcaa tctcatgaac atgaaccatg ccctgctgga tgctttgggt gttggtcacc
     2761 cggttctctc gaaacttgtt cttgcagcac gttctgcggg agcccacgga gcaaagacta
     2821 ccggagccgg cggaggcggg tgtatgtatg ccatctgttc aaagggcgca cgcaaccggg
     2881 tcgccggtgc cattgagggc tgcgatgcac gcgccatcat caccacgatt gacacggaag
     2941 gtgcacggaa ggaaaaagat gagtgaaatt accgtactca aacttggcgg aagtgttgtt
     3001 acaaaaaaat ccgaggctgg tgtaattg
//
LOCUS       DALTZZ010000266         2351 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_45563,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000266 DALTZZ010000000
VERSION     DALTZZ010000266.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45563"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            103..609
                     /locus_tag="O0X12_05145"
     CDS             103..609
                     /locus_tag="O0X12_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJK53570.1"
                     /translation="MPKTLRIPFVRDDPILIRGCSSFLLVARSREKFPLIIETDDEEL
                     VQGVYPSDLIVVSAPEGGEIPPALYLLEMVKTYHTPVIALGKDHPASRRLSYVISAAE
                     RIEMRCDIRRGTHPEQHLLCAADEFTGMILYAEEEDLVIENCREELRLSYMTWNLSLA
                     ETTYQNCG"
     gene            complement(741..>2351)
                     /locus_tag="O0X12_05150"
     CDS             complement(741..>2351)
                     /locus_tag="O0X12_05150"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HJK53571.1"
                     /translation="AEIAVIMQIGSYLEERTVAKARAGIEKLVDLSPQTARVVRNGGE
                     EIIPSDDVRVGDVLRVLPGETIVVDGVVLSGTTSVDQSVMTGESLPVDKQSGDEVFSG
                     TVNQFGSFDMKATKDGADSSLARMIQLVTSADAGKAEIVRTADRWATWEVAAALTVSV
                     LTYFVTGDILRSVTVLVVFCPCAMVLATPTAIMAGIGNLTKYGILVREGDALERLNIV
                     KYLMFDKTGTLTYGTPEVGEVIPVDPEISADDLLRLAASAEVRSEHPLGKSIIRKYQE
                     KYGSAPEQPEAFSMLPGRGISAMVHGREILIGNTELLSGNSISVPQDAVSTVDACLQD
                     GATVMYVVVDGRFTGFISLSDTLREDAPVVVRELKAMNIRPVLLTGDNQNAASHIAEV
                     IGIDDVHAECLPAKKLEMIDWYQESGSKVCMIGDGVNDAPALKRSYVGIAMGGIGSDI
                     AVDAADIVLIHDNIGNLPHLLGLAKQVIRTIHVNLALALLLNFVAIILAVLGIIDPIV
                     GALIHNVGSVLVIMNSALLLGWKYKQTA"
BASE COUNT          617 a          623 c          561 g          550 t
ORIGIN      
        1 caaaaaatac cggtgcaacg agtgcggcca gacatttacc ggccttggca agcatccgat
       61 gtgcccttcc tgccagtccg aggacgtcac tgagatataa caatgccaaa aactctccgt
      121 atcccgtttg tgcgggacga tccaatcctc atcagaggat gctcttcttt tttgctcgtt
      181 gcacgttccc gggaaaagtt tccgttgatc atcgaaaccg atgatgaaga acttgtgcag
      241 ggagtgtacc cgagtgatct gattgtggtc tctgctcccg aaggtgggga aataccacct
      301 gctctgtatc ttttggagat ggtaaaaacc tatcacacgc cggtgatcgc cttaggaaaa
      361 gatcatcccg caagcaggag actctcatat gtcatctcag ctgcagaacg gatcgagatg
      421 cggtgtgaca tcagacgcgg tacgcatcca gaacagcatc ttctctgcgc cgcagatgag
      481 ttcaccggaa tgattctgta tgccgaagaa gaggatctgg tgatcgaaaa ctgtcgtgaa
      541 gaactcagac tgtcatatat gacctggaac ctctccctcg cagaaacaac gtaccagaac
      601 tgcggctgaa cgctcctctg tctcacatcc ctgacacatt ttttgttgga gaatggtgat
      661 caaacaattc ctactgtgtg gagatgagct acacggctgg ctcctgctga ccagaacaaa
      721 agaaaaaaac agggaggaag ttatgcagtt tgtttgtact tccatccaag caggagggca
      781 gagttcataa taaccagaac ggagccaacg ttgtggatca aagcaccgac aatcgggtca
      841 atgatgccaa gtaccgcaag aatgatcgct acgaagttca gcagcaatgc aagtgccaga
      901 ttcacatgga ttgttctgat aacttgcttc gcaagaccca gtaggtgcgg gagattgccg
      961 atattatcgt gaataaggac aatgtcagct gcatccaccg cgatgtcact gccaattcct
     1021 cccatagcaa tgccgacata cgaacgtttc agggcagggg catcgttgac gccgtctccg
     1081 atcatacaca ctttgctgcc actttcctga taccagtcga tcatctccag tttctttgcg
     1141 ggaagacatt cggcatgcac atcatctatc ccgatcactt ccgcaatgtg ggatgccgca
     1201 ttctgattat ccccggtcag cagaacagga cggatgttca tcgctttcaa ttcccgaacc
     1261 actactggtg cgtcttcccg aagagtatct gagagggaga taaatccggt gaaccgcccg
     1321 tccactacaa catacatcac cgtcgcaccg tcttgaagac acgcatcaac agtagatacc
     1381 gcatcctgcg gtacagaaat actgttaccg ctgagaagtt cggtattgcc gatgagaatt
     1441 tcacgaccat gaaccattgc cgagatacca cgtcccggca gcatggaaaa tgcctctggt
     1501 tgttccggtg cagaaccgta cttctcttgg tactttcgga tgatcgattt gccaagcggg
     1561 tgttccgatc gtacttcagc agatgcggca agtctcaaca aatcatctgc tgagatctcc
     1621 ggatctaccg ggatcacctc accgacctca ggggttccgt aagttagtgt tccggttttg
     1681 tcaaacatca ggtacttgac aatgttcagc cgctcaaggg catcgccttc acgaacaagg
     1741 ataccgtatt tggtgaggtt gccgatacct gccataattg cagtaggtgt tgcaagtacc
     1801 atggcacagg ggcagaatac aacaagtacg gtaacggaac ggagaatgtc accggtaaca
     1861 aagtacgtca gaaccgacac agtcagagct gctgcaactt cccatgttgc ccacctatct
     1921 gctgtccgaa cgatctctgc ttttccggca tccgcggatg taacgagctg aatcattctg
     1981 gcaagagagc tgtctgcacc atccttcgtt gccttcatgt caaaagaacc gaactgattg
     2041 actgttccgg agaaaacttc atcgcccgac tgtttgtcaa cagggagtga ttcaccagtc
     2101 atcactgatt gatcaaccga ggttgtgccg gagagaacaa caccgtccac gacgatggtt
     2161 tcaccgggaa gaacgcggag aacatcaccc acccggacat catcagacgg aataatttct
     2221 tctccaccat tacggacaac acgtgccgtc tggggcgaga gatcaaccag tttttcaata
     2281 ccggcacgcg ctttggcaac agtccgttcc tcaagatacg atcctatctg catgatgacc
     2341 gcaatttctg c
//
LOCUS       DALTZZ010000267         6285 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_46005,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000267 DALTZZ010000000
VERSION     DALTZZ010000267.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46005"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="O0X12_05155"
     CDS             <1..1064
                     /locus_tag="O0X12_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04083 family peptide-modifying radical SAM
                     enzyme"
                     /protein_id="HJK53572.1"
                     /translation="LETADAIVRWLEPLEDQRVTFTFHGGEPLLAGPEFYRYILPKIS
                     SRLAHLSPEFAMQTNLWLMTDELAEILAEYHVPLGSSIDGPETLTNYQRGDNYFARTM
                     AGYRTAISHGLLVRFICTFTNSSVLQKEEIVRFFREQGWVMKLHPALPSLKGDNPNEW
                     TLSPAEYGELLVYLLDEALLHADEFEIMNINDLCRCVFTRSGSVCTYADCMGTTFAVG
                     PDGAIYPCYRFVGMPEWVMGSVFDAPTIDDLMVSPAGKRMLAFKDHVDTACRSCAHID
                     YCRGGCPYNALAGTSEHVEGVDPHCMAYKRIFDEITTRMEHEMAAETAVPTPAARMSR
                     VRRKAKPGVMALVKKIVEQ"
     gene            complement(1207..1280)
                     /locus_tag="O0X12_05160"
     tRNA            complement(1207..1280)
                     /locus_tag="O0X12_05160"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1244..1246),aa:Val,seq:gac)
     gene            complement(1624..2133)
                     /locus_tag="O0X12_05165"
     CDS             complement(1624..2133)
                     /locus_tag="O0X12_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HJK53573.1"
                     /translation="MNFKNLLPAKNRDELRQWLLENHDKEAECWVVVKRGRPVDKDTF
                     WYIDAVEEAMCFGWIDSTTKKMDNGITAQRLAPRRKGSVWSELNKERCRRMERLGRMT
                     DAGRAVLPDMSESGFVIDKDILMALQADAEIWENFQKFPPLYQRVRVDTIQIKKSNRI
                     YSKAVCKNY"
     gene            complement(2283..3866)
                     /gene="serA"
                     /locus_tag="O0X12_05170"
     CDS             complement(2283..3866)
                     /gene="serA"
                     /locus_tag="O0X12_05170"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HJK53574.1"
                     /translation="MSFKVLVSDPLAKEGVDILKGFCDVDEKADLSEDELVKIIGEYD
                     ALIVRSGTQVTARIIEAADKMKYIGRAGVGVDNIDCDAATKKGIIVSNAPEGNTLAAT
                     EHTIAMMMALARNIPQATASLKKGEWKRSKFMGNEMNGKVLGVVGFGRIGREVSKRAQ
                     ALQMTVIAYDPFIPAEVGAAMGVEMMSVADLFTKADVITVHTPLIPSTTHLINKESIA
                     TMKDGVRLINCARGGIIDEAALYEAITSGKVAGAALDVFEQEPPTNSPLLSLDKVIVT
                     PHLGASTVEAQKNVAISVAQQCIEVLKGGSAKNAVNAPLVAPELRPKLEPFALLTEKM
                     GCLAAQLADSAITEVEFAYLGEVAEMKQNLKYPTRMGLKGLLDKALHEPANLVNAEFI
                     AQQRGMTVVEKTSADAGDYKSLVTLTFKTEKGTQTISGIITRVGPRITSINGYSMDLV
                     PKGNVIVADHINRPGVVGPVCVLLGKHNINISNMQVGKVDVGSESLMILAVDDVVPAA
                     VMQEVAGSDGIISAKFMQL"
     gene            3998..6283
                     /gene="ppsA"
                     /locus_tag="O0X12_05175"
     CDS             3998..6283
                     /gene="ppsA"
                     /locus_tag="O0X12_05175"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HJK53575.1"
                     /translation="MDKSPNILWLNEIRKDDIPSVGGKGASLGEMTSIGLPVPQGFVV
                     TAQAFRRFLQLTTLEDSIFDILEKLDVDDNDALNATADLVKSMVSSAVMPEVIKKEII
                     ASYQKMGSDTVVAVRSSATAEDLPDASFAGQQDTYLNILGETDLLQAVQDCWASLYGG
                     RAIYYRAKQGFDDRTVNIAVVVQQLVFSEKAGVMFSSHPISGAATVIIEGSWGLGEAI
                     VSGTVSPDNYVYDRKNKRITTKTVANKSVEIIPDGNKGTKTVPVPESRQNVQVLSDVE
                     VAKLAEFAVISEEHYKVPQDMEWGMVGDTIYILQSRPITTIQKNHVVSASENTADSAA
                     DGEIILQGYGASPGIASGPVVIVSDAKDTSRVKEGDIMVSPMTNPDMVPAMRKVVGII
                     TDEGGMTCHAAIVSRELGTPAVVGTKKATSLLKEGQIVTIDGEKGLIYDGKLEAASPV
                     TAAVAAPVAAAAPIITATSVKVNVSMPEAAARAAATGADGVGLLRIEHLIIGMNKTPS
                     WYIKNGKEEEFISELYNGIKTVLDAFRGKPVWVRTIDSPTDEFLNMAGGEDEPHEHNP
                     MLGFRGLRRDLRQMAQFKMQAECFKRLWEAGYDNLGIMFPLVQHPREFLAAKEAFRSW
                     GIDVENRVLGIMVEIPASAIIIDEFIKAGIKFCSFGTNDLIQYTLAVDRNNGMIGDMY
                     EPEHPAVMRLIKHCIEQCREAGVECSICGQAGSDPKFVKWLVNQGISSVSSNIDAIPK
                     IRETVAKTERQILLNMAFKKN"
BASE COUNT         1463 a         1571 c         1566 g         1685 t
ORIGIN      
        1 cccttgaaac cgctgatgcg atcgtgcgat ggctggaacc gctcgaggat cagcgggtga
       61 ctttcacctt ccatggcggt gagccgctgc ttgcaggtcc tgagttctac cgctatattc
      121 tcccgaagat ctcttcccgt cttgcgcatc tgtctccgga gtttgcgatg cagacaaatc
      181 tctggctgat gaccgacgaa cttgcggaga ttcttgcaga gtatcatgtg ccgctcggct
      241 cctccatcga tgggccggag acattgacca actatcagcg tggtgacaac tattttgcac
      301 gaacgatggc aggctacagg actgcaatct cgcatggtct tctcgtgcgg ttcatctgca
      361 cctttaccaa ctcctctgtt ctgcagaagg aggagattgt cagattcttc cgtgaacagg
      421 ggtgggttat gaaactgcat cctgccctgc cgtccctgaa aggggataac ccgaatgagt
      481 ggaccctttc acctgcagag tatggggagc ttctggttta tctgctggac gaagcgcttc
      541 tgcatgctga tgagtttgag attatgaaca tcaatgatct ctgccggtgc gtgtttacgc
      601 ggagcgggag tgtctgtact tatgcagact gtatgggaac aacattcgcc gtggggccgg
      661 acggtgcaat ctatccctgc taccggtttg tgggaatgcc tgagtgggtg atgggatctg
      721 tctttgatgc accgaccatt gatgatctga tggtatcccc cgcaggtaaa aggatgctcg
      781 ccttcaaaga tcatgtggat acggcctgcc ggagctgtgc ccatattgat tactgccgtg
      841 gcggctgtcc atacaatgcc cttgccggaa catcggaaca tgtggaagga gttgatccgc
      901 actgtatggc atacaaacgt atctttgatg agataaccac ccggatggag catgagatgg
      961 cagcagaaac agcagtccca actcctgcgg cacggatgtc gcgtgttcgg cgcaaggcaa
     1021 agcctggggt tatggcactg gtaaaaaaga tcgttgagca gtaactggat gctagttcat
     1081 ccctcttttt ccaagacttt tagtggcaca tccttttttt gtactactga tttcttttgt
     1141 tggcggatag aacggataat gctggatgaa taacacagta atttctgaat aatcttagaa
     1201 aagaagtgga cccgaaggga gtcgaaccct tgatctccgc catgtcaagg cgacgtcata
     1261 gcctactaga ccacgagtcc atggatgttt cgcacctatg tgctctaata catatgatat
     1321 tctgaaatat aaatatttct aatggtctct ttatcagtcc ctattttggt gctgtgcatt
     1381 tccactccaa tattacttcc tgtatttaga tctacgtaga atatcatcca gtcgttcctt
     1441 tctgcacgtt tcaaacattt ctttctggat agtatcgcct catatggccg ggacttcgtt
     1501 ttgaaaaatt tgaatgggcc tgacgacggt tactatgcca aatgggaagt tatttagtgg
     1561 tattatcaag caaacgcccg ttgtcgttcc attccccata catgacgcca gcttttgtgt
     1621 tgtctaatag tttttgcaaa cggcttttga ataaatccgg ttgctttttt tgatctgaat
     1681 ggtatctact cttactcttt ggtatagcgg gggaaatttc tgaaaattct cccaaatttc
     1741 agcgtcagct tgtagtgcca ttaaaatgtc cttgtcgatg acaaaaccac tttcggacat
     1801 atctggtagc acggctctgc ctgcatctgt catgcgccct aatcgttcca ttctccggca
     1861 gcgttctttg tttaattcag accacacgct gccttttctt cgaggcgcta atctctgcgc
     1921 ggttatgcca ttatccattt tcttcgtggt actgtcgatc cagccaaaac acatagcttc
     1981 ttctactgcg tcaatgtacc aaaatgtatc tttgtcaacg ggccgtccac gctttacaac
     2041 tacccaacat tcagcttcct tgtcgtgatt ttccaaaagc cattgtcgta attcatcacg
     2101 atttttagct ggcaataaat ttttgaaatt catttgtgtg tacctcttac ttttcgatgt
     2161 atcacggact atattcgatt cagatatacc acttttggaa atggatgaat gctaaccttg
     2221 ctggattgga gtagtgttca gtgtgttgtc aaaaacattg gtaaaaaaag tttagagggt
     2281 gattacagct gcatgaactt tgcagagatg atgccgtcgc ttcctgcgac ttcctgcatc
     2341 accgctgccg gaacaacgtc gtccacggca agaatcatca gtgactcgga accaacatcc
     2401 acctttccca cctgcatgtt cgagatgttt atgttatgtt taccaagcag cacgcacact
     2461 gggccaacaa ctccggggcg gttgatgtga tctgcaacaa tcacgttgcc tttcggcaca
     2521 aggtccatgg agtaaccgtt gatagaggta atacgtggac cgacacgggt aataattcct
     2581 gagatcgtct gcgtaccctt ctcggtctta aaggtcagcg taaccagaga cttgtagtct
     2641 cccgcatcag ccgaagtctt ctcaacaacg gtcataccgc gctgctgtgc aataaactcc
     2701 gcgttcacca ggtttgctgg ctcgtggagc gccttgtcca gaagaccctt cagacccatg
     2761 cgtgtggggt acttcaggtt ctgtttcatc tcggcaacct cgccgaggta tgcgaactca
     2821 acctcagtga tcgcgctgtc tgcaagctgt gctgcaaggc atcccatctt ctcggtaagc
     2881 agtgcgaacg gctcaagctt cgggcggagc tccggtgcaa ccagcggtgc attcactgca
     2941 ttcttggcag aacctccctt cagcacttca atacactgct gtgcaaccga gattgccaca
     3001 tttttctgtg cctcaactgt acttgcaccg aggtgcggtg tcacaattac cttatcgagg
     3061 gaaagaagcg gtgagttggt tggcggttct tgctcaaaca catcaagtgc agctcccgca
     3121 accttgcctg aggtaattgc ctcatagagg gctgcctcat caataatacc accacgggca
     3181 cagttgatta aacggacacc gtccttcatg gttgcaattg attccttgtt gatgagatgt
     3241 gtcgtggacg gaatcagcgg cgtgtgtact gtaatcacat cggctttggt aaagagatca
     3301 gccacactca tcatctcaac acccattgcg gctccgacct ctgccgggat gaacggatcg
     3361 taggcaatga ccgtcatctg cagtgcctgg gcccgtttgg aaacttcgcg cccgattctt
     3421 ccaaacccaa ccacgccaag cactttgccg ttcatctcgt tgcccatgaa ctttgaacgc
     3481 ttccactcgc ctttcttcag tgatgcggtt gcctgcggga tatttctcgc aagagccatc
     3541 atcatggcga tggtgtgttc cgttgccgcg agcgtgttgc cttctggagc gttggagaca
     3601 ataatgcctt tctttgtggc cgcatcacag tcgatgttgt ccacaccgac accggcacga
     3661 ccgatgtact tcattttgtc ggccgcttcg atgatacgtg cagtgacctg tgttccggat
     3721 cggacaatca gtgcatcgta ctcgccgatg atcttgacga gttcatcctc ggacaggtcc
     3781 gccttttcat caacatcaca aaatcccttt aaaatatcga ctccttcttt tgcgaggggg
     3841 tcgctcacaa gtactttgaa gctcacggat gatcccgtat ctatttgttc ttactcggta
     3901 atagtattgt tcaacccaag ggggtgcatc gcagtgtttc tccaactacc aacacaatat
     3961 attattgttg ccgtgaccaa tgttatattg agtgtctatg gacaaatcac ccaatatcct
     4021 atggctcaac gaaatccgca aagatgatat tccctccgtt ggaggaaaag gcgcctctct
     4081 tggtgaaatg acctctattg gtctccctgt cccacagggt tttgtcgtga ccgcccaggc
     4141 tttccgcaga tttttacagc ttacaacact ggaagactct atcttcgata ttctggaaaa
     4201 acttgacgtt gacgacaacg atgctctcaa tgctaccgca gatctggtca agtccatggt
     4261 tagttcggcg gtcatgcccg aagtgatcaa aaaggagatc attgcttcct atcagaagat
     4321 gggatccgac accgttgttg cagtccggtc cagtgcaacc gcagaggatc tgccggatgc
     4381 ctcctttgcc ggtcagcagg atacctatct gaatatcctc ggtgaaaccg atctcctgca
     4441 ggcagtgcag gactgctggg cttcccttta tggaggtcgt gccatctatt accgcgcaaa
     4501 gcagggtttc gatgaccgta ccgtcaacat tgcggtggtt gtccagcagc tggtgttctc
     4561 ggaaaaggca ggtgtgatgt tttcatctca tccaatctca ggtgctgcaa ccgtcatcat
     4621 tgagggttca tggggtcttg gtgaggcaat cgtatccggt actgtgtcac ctgacaacta
     4681 tgtttatgac cgcaagaaca agcggatcac cacaaagacc gttgccaaca aatctgttga
     4741 gatcattccg gacggtaaca aaggaacaaa gactgtccct gttccggaga gccgccagaa
     4801 tgttcaggtt ctttctgacg ttgaggttgc aaaacttgct gagtttgctg tcatctctga
     4861 ggagcactat aaagttccgc aggatatgga gtggggaatg gtgggcgaca ctatctatat
     4921 tctccagtcc cgcccaatca ctacgattca gaagaatcat gttgtctccg cgtccgagaa
     4981 tacagccgat tctgcagcag acggtgagat tattcttcag ggatacggtg cttcccccgg
     5041 aattgcatca ggtccggtcg ttatcgtttc cgatgcaaag gacacctccc gtgtaaaaga
     5101 gggtgacatc atggtctccc cgatgaccaa cccggacatg gttcctgcta tgcggaaagt
     5161 ggtgggcatc atcactgatg aaggtggtat gacctgtcat gcggcaattg tgtcgcgtga
     5221 gctgggaact cctgcggttg tcggtacgaa aaaggcaacc tctcttctga aagaaggaca
     5281 gattgttacc atcgatggtg aaaagggtct gatttacgac ggtaagcttg aggcagcatc
     5341 acccgtgaca gccgcggtag cagcaccggt tgcagctgca gcacctatca tcaccgcaac
     5401 ctcagtgaag gtaaacgttt caatgcccga ggcagcagca cgtgcggcag caacgggtgc
     5461 tgacggtgtg ggtctgctga gaatcgagca cctgatcatc ggcatgaaca agactccgag
     5521 ctggtacatc aagaacggca aggaagaaga gttcatctcc gaactgtata atggtatcaa
     5581 gacggtgctc gatgctttcc gcggaaagcc tgtctgggtg cgtactatcg attctccgac
     5641 cgatgagttc ctgaacatgg caggcggaga ggatgagccg cacgaacaca acccgatgct
     5701 tggtttccgc ggtctgcgcc gtgatcttcg ccagatggca cagttcaaga tgcaggcaga
     5761 gtgtttcaag cgtctgtggg aggcaggata cgacaacctc ggtatcatgt tcccgctggt
     5821 acagcaccca cgcgagttcc ttgcagcaaa ggaggcgttc cgttcctggg gtattgatgt
     5881 ggagaatcgt gtgcttggta tcatggttga gattcccgca tctgctatca ttattgatga
     5941 gttcatcaag gcaggtatca agttctgttc ctttggaaca aacgatctga tccagtacac
     6001 ccttgccgtg gaccgcaaca atggtatgat cggtgatatg tatgagccgg aacacccggc
     6061 tgtgatgcgc ctgatcaaac actgtatcga acagtgccgt gaggcgggcg ttgaatgttc
     6121 catctgtggt caggcaggat cggatccgaa gttcgtgaaa tggctggtga atcagggaat
     6181 ctccagtgtc tcctcaaaca ttgacgcaat tccaaagatt cgtgagacgg tcgcaaagac
     6241 tgagcgccag attctgctga atatggcatt taagaagaac taata
//
LOCUS       DALTZZ010000268         1936 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_46120,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000268 DALTZZ010000000
VERSION     DALTZZ010000268.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46120"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /gene="fmt"
                     /locus_tag="O0X12_05180"
     CDS             <1..964
                     /gene="fmt"
                     /locus_tag="O0X12_05180"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HJK53576.1"
                     /translation="RKAGGDSMRVLFMGTPAYAVPALEAVASRHEIVGVVTRVDKPNR
                     RGNKIAFSPVKEWALAHEVPIFQPESMRDPALAGELAALAPDVGVVVAFGMMIPEEIA
                     NLPKHKTINIHGSLLPRYRGAAPMQYSVLNGDPEAGVSIMYITQELDAGDVILSRSLD
                     VGEDETFGELHDRLAELGASALMEALDLISCGGVCAVPQDPAQATFAPSITKEECVVD
                     WTLPAGMVHNRIRGLSPAPCANTRLPDGKRLLIYRSMLVPHAPAGVPGEVVDVIKKKG
                     PVVMTGAGAVCILSAKPEGKREMPGFELVNGHYLSVGERLGSQD"
     gene            1078..>1936
                     /locus_tag="O0X12_05185"
     CDS             1078..>1936
                     /locus_tag="O0X12_05185"
                     /inference="COORDINATES: protein motif:HMM:NF015380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HJK53577.1"
                     /translation="MTAAVLLLLLAVACVTPVTAADTSGDDAKNYLIIYIIGSNLEAE
                     YGEMSGLMQDMTANWDPNAGEMLIIYGGADKSGWDSGITITNASLLFSDLAKGSGNVT
                     EDGEVLERLAADISTPEGMQQALSFAAAYQQDSGLTDAHTYLFFLDHGGGATGYGMNT
                     VTGRMLSLPDIKTGLSDRTYDMIVMHACLMGTVESLAVLDDHADYLIAAEQIVTQDAV
                     NYTELGRVLSADPSISPKELGKHIIRTAAADEVSTYALLDAGNVPEVVDALNAFGAAL
                     NASMISSNTV"
BASE COUNT          406 a          527 c          598 g          405 t
ORIGIN      
        1 ccgcaaagca ggaggagaca gcatgagagt tctgttcatg ggaacacctg cctacgctgt
       61 tccggcactg gaagcggttg cctcccgcca tgagattgtg ggcgtggtga cgcgggtgga
      121 caaacccaac cggcggggaa acaagatcgc gttttcgccg gtgaaggagt gggccctggc
      181 ccatgaggtc ccgatcttcc agcctgagtc gatgcgggac ccggcactcg ccggagaact
      241 tgcggcactt gcaccggatg tcggtgtggt ggtggcgttc gggatgatga tccccgaaga
      301 gatcgcgaac ctcccgaaac ataagacgat caacatccac ggttctcttc ttccccggta
      361 ccgcggggcg gccccgatgc agtactcggt actgaacggg gacccggagg caggtgtgtc
      421 tattatgtac attacgcagg aactggatgc gggggacgtg attctatcgc ggtcgctgga
      481 tgtcggcgag gatgagacgt tcggcgagct gcacgaccgg cttgcggagc tcggggcgtc
      541 ggcactgatg gaagcgctgg atctgatctc ctgcggcggt gtgtgtgcgg ttccgcagga
      601 tccggcacag gcgacgtttg ctccgtcgat caccaaggag gagtgcgtgg ttgactggac
      661 actgccggcg ggcatggtgc acaaccgtat ccggggtctg tcgccagcgc cttgtgcgaa
      721 tacgcggctg ccggacggaa agcggttgct gatctaccgg agtatgctgg tgccgcatgc
      781 acctgcgggt gttcccggcg aggtggtgga cgttattaaa aagaaggggc cggttgtgat
      841 gaccggtgcc ggggcggtgt gtattttgtc ggcaaaacct gaaggaaagc gggagatgcc
      901 gggatttgag ctggtgaacg gacattacct gagcgtgggc gaacggctcg gctcgcagga
      961 ctaactcctt tttctttttt cttcctcatg agatggattc atatacaatg ctgatcttat
     1021 tccctgatgc aagggaggaa ccactatttg atccccataa atccgtattt cctcaggatg
     1081 acagcagcag tacttctcct gctgctggct gttgcctgtg tcacacccgt caccgctgcc
     1141 gacaccagcg gtgatgatgc gaagaactat cttatcatct acatcatcgg ctccaacctc
     1201 gaggcagagt acggcgagat gtccggcctg atgcaggaca tgaccgcaaa ctgggacccg
     1261 aacgcaggcg agatgctgat catctacgga ggagccgata aaagcggctg ggactccggc
     1321 atcacgataa ccaacgcgtc gcttttgttc agtgatctgg caaaaggcag cggcaatgta
     1381 accgaggacg gcgaggtgct ggaacggctt gcagcagaca tctccacacc tgaaggtatg
     1441 cagcaggcgc tctcgtttgc tgcggcctac cagcaggaca gcggtcttac cgatgcacac
     1501 acctatctct tcttcctgga ccacggcggc ggtgccaccg gttacggaat gaacaccgta
     1561 accggcagga tgctctccct cccggacata aaaaccggtc tttcggatag gacgtatgat
     1621 atgatcgtca tgcacgcctg cctcatggga acggtggagt cgcttgccgt tcttgacgat
     1681 catgccgatt acctgattgc agcagaacag atcgtcaccc aggatgcagt aaattatacg
     1741 gaacttggcc gtgttctctc ggcagatccg tccatctctc caaaggaact gggaaaacac
     1801 atcatccgga ccgccgccgc ggatgaggtg agcacgtatg cactgcttga tgccggaaac
     1861 gtaccggaag tggtggacgc actgaacgcg tttggtgcag ctctcaacgc ctccatgatc
     1921 tcttccaaca ccgttc
//
LOCUS       DALTZZ010000269         3827 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_46164,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000269 DALTZZ010000000
VERSION     DALTZZ010000269.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46164"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..89
                     /locus_tag="O0X12_05190"
     CDS             <1..89
                     /locus_tag="O0X12_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK53578.1"
                     /translation="LDFCKGCGMCAEECPDKAKAITMHKEEK"
     gene            90..1247
                     /gene="porA"
                     /locus_tag="O0X12_05195"
     CDS             90..1247
                     /gene="porA"
                     /locus_tag="O0X12_05195"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorA"
                     /protein_id="HJK53579.1"
                     /translation="MTLQIMEGSIAVAETVRLCRPQVISAYPITPQTHIVEGLASIVA
                     DGRLDAEYICVESEFSALSCCIGASAAGSRVYSATTSQGLALMGEVVFNAAGMRLPIV
                     MGIANRAISAPLSIWNDQQDSIMMRDSGWLQFYAEDNQEAVDLHVLAYKICEDHNILL
                     PAFVCFDGFILSHVFEPVDIPSQEEVDEFLGPFTPYQKLDVNDPISFGMYATPEYYLE
                     FRYEHDQAVHRAADALRKYGKAFGEKFGRDYSSLVEGYKLEDAEIAIVAMGSICGTTK
                     DAIDEMRAEGKKVGLLKIRCFRPFPAEDIAKALAHVNTIAVLDKNVSLGAKGAVAIEI
                     KEACYGSGIPVYDYILGLGGRDVRKKDIKHIVELAEKGSGDMFYGLREELL"
     gene            1248..2138
                     /locus_tag="O0X12_05200"
     CDS             1248..2138
                     /locus_tag="O0X12_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJK53580.1"
                     /translation="MVDRTIENFDCGHRACGGCGASSAVRMILKGAGENTIVVSPTGC
                     LEVFSTPYPETAWKTPWIHSLFENASAVASGIEASLKKQGRLGKEKILVIGGDGATVD
                     IGMLCISGAFERGHDFTYVCYDNEAYMNTGIQRSGATPYDADTTTSPSGACSTGNNRP
                     KKDLPQILVAHGSPYVATATMAYPMDLMKKVEKAMNTPGPCYIQVHAPCCTGWGFDGS
                     KTMEIGRMAVQCGLWPCYEMVNGELVSAKKVKRVPVDEYLKAQKRFRHLFKPTRNDEE
                     IAKIQAIADANAAKYGVDIE"
     gene            2189..2836
                     /locus_tag="O0X12_05205"
     CDS             2189..2836
                     /locus_tag="O0X12_05205"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HJK53581.1"
                     /translation="MTFAADILSLLFHPKEFFTDTSRTHSLKLPLLYTAVYGIFAAVL
                     GYQTTAASAELIGMPDYAGVLGTVGAVTGFVMAFIVWVFVAVFFFVFLKFITHTATGF
                     KPFLTVTGYGSLPVLIGTVLTLLAGAVTPGVFDGWAGYLLNAAVLLWCMPIWIYGFAS
                     AGNVPVHAVFTALLIPILIMAAYTAYQTYTTIESLKTLAETGSIGGMQFSMQSPR"
     gene            complement(2980..3585)
                     /locus_tag="O0X12_05210"
     CDS             complement(2980..3585)
                     /locus_tag="O0X12_05210"
                     /inference="COORDINATES: protein motif:HMM:NF039237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5803 family protein"
                     /protein_id="HJK53582.1"
                     /translation="MVPASANTASYYISENGTAFSAEVTLVNQSGYLLISPGFIGEGA
                     ELQVSNLTLTAEDGTIVNATKQSSTQITFPEGNYTLVYDAPLDGRLMYAQYLSPFNTT
                     VFLPAGFHTNNLILGPVSNDGKTYLAAEDMDQLHEMGITVDAAKYETVVVWTDKRAIQ
                     MRFYPASYEQYFILCMGLWLIIFCAVGYRYWRLKKNENRYT"
     gene            complement(3618..>3827)
                     /locus_tag="O0X12_05215"
     CDS             complement(3618..>3827)
                     /locus_tag="O0X12_05215"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833789.1"
                     /note="catalyzes the formation of L-threonine from
                     O-phospho-L-homoserine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HJK53583.1"
                     /translation="RYEGIGVEPASAASVAGIRKLAEQGVLDPNEKIVCVVTGHLLKD
                     PETVIKQCNPPIEIDPTIEALLSVL"
BASE COUNT          944 a          985 c          981 g          917 t
ORIGIN      
        1 atctcgactt ctgcaaaggc tgcggcatgt gtgcggaaga gtgtcccgac aaggcaaagg
       61 caatcaccat gcacaaggag gaaaaataaa tgactcttca gattatggag ggatccattg
      121 ctgttgcaga gaccgtacgg ctttgccgcc cgcaggtcat ctccgcatac ccgatcaccc
      181 cgcagaccca cattgtagaa ggactcgcat ccatcgttgc ggacggacgg cttgatgctg
      241 agtacatctg tgtggagtcg gagttttctg cactttcctg ctgtatcgga gcatctgctg
      301 ctggcagccg tgtgtactct gcaaccacat cccagggtct tgcactgatg ggagaggttg
      361 tctttaacgc tgctggaatg cggctcccga ttgtgatggg cattgcaaac cgtgcaatct
      421 ctgcgccgtt atcgatctgg aatgatcagc aggactccat tatgatgaga gattccggat
      481 ggctgcagtt ctatgcagag gacaatcagg aagcagttga tctgcatgtt cttgcctaca
      541 agatctgtga agaccacaac atcctgcttc cggcattcgt ctgctttgac ggattcattc
      601 tttcacatgt attcgagccg gttgacatcc catcgcagga agaggtggac gagttcctgg
      661 gtccgttcac cccgtaccag aaacttgacg tgaacgatcc gatctcgttt ggtatgtatg
      721 caacacctga gtactacctt gagttccggt acgaacacga tcaggcagtg caccgtgcag
      781 cagatgcact gcgcaagtac ggcaaagcct tcggcgaaaa gttcggccgt gactactcca
      841 gccttgtaga ggggtacaaa ctcgaggacg cggagatcgc aatcgttgca atgggttcca
      901 tctgcggcac caccaaagat gcaatcgacg agatgcgtgc agagggtaag aaggtcggtc
      961 tcttaaagat ccgctgcttc cgtccgttcc cggcagagga catcgcaaaa gcacttgccc
     1021 atgtaaacac cattgccgtg ctggacaaga atgtcagtct tggtgcaaaa ggagctgtag
     1081 ccattgagat caaggaagca tgctacggtt ccggcattcc ggtctatgac tatatcctcg
     1141 gtctcggcgg ccgcgatgta cgcaagaagg atattaaaca cattgtagaa cttgccgaga
     1201 aaggttccgg cgatatgttc tacggactgc gcgaggagtt gttataaatg gtagatcgta
     1261 caattgaaaa cttcgactgc ggtcaccgcg catgcggcgg atgcggtgca tcttctgcgg
     1321 ttcggatgat cctgaaagga gccggagaaa acaccatcgt cgtgagtcca accggatgtc
     1381 tggaagtgtt ctccactcca tacccggaga ccgcatggaa gacaccctgg attcactcac
     1441 tgttcgagaa cgcatccgcg gttgcgtccg gtattgaggc atccctaaag aagcagggac
     1501 gcctcggaaa ggaaaagatc ctcgtcatcg gcggagacgg cgctactgtg gatattggta
     1561 tgctctgtat ctccggagcg ttcgagcgcg gtcatgactt cacctatgtc tgctatgaca
     1621 atgaagccta catgaacaca ggtatccagc gttccggtgc aacgccgtac gacgcagaca
     1681 ccaccacgtc accttccggt gcatgctcaa ccggaaacaa ccggcccaag aaggatctcc
     1741 cgcagattct ggtcgcacac ggatctccgt atgtggcaac tgcaacaatg gcatacccga
     1801 tggacctgat gaaaaaagtc gagaaagcta tgaacactcc cggtccctgt tacatccagg
     1861 tacacgcacc ctgctgtacc ggatggggat ttgacggatc aaagacgatg gagatcggaa
     1921 gaatggcggt ccagtgcggt ctgtggccgt gctatgagat ggtgaacggt gaacttgtct
     1981 ccgcaaagaa ggtcaagcgt gtaccggtcg atgagtatct caaagcccag aagcggttcc
     2041 gccacctgtt caaaccgacc agaaatgatg aagagatcgc aaagatccag gcaattgccg
     2101 atgccaacgc cgcaaagtac ggtgttgaca ttgaataact cttttttttc caagtactca
     2161 tatccaaaga gaacgaatat agactgtaat gacgtttgca gcagatatcc tctccctgct
     2221 attccatcca aaagagttct ttacagatac gtcccgtacg cactctctca aacttccgct
     2281 gctctataca gcagtctacg gaatttttgc agcagttctc gggtatcaga caactgcggc
     2341 atccgcagaa ctcatcggca tgcctgacta tgcaggagta ctcggtacag ttggtgcggt
     2401 aaccgggttt gtgatggctt tcattgtatg ggtttttgtc gcggtatttt tctttgtgtt
     2461 tctcaagttc atcacgcaca ccgctacggg tttcaaacca ttccttacag ttaccgggta
     2521 cggatccctg ccggttctga tcggaactgt acttacgctt cttgcaggtg cggtcacacc
     2581 gggtgtcttt gacggatggg caggatacct gctgaatgct gctgttctcc tctggtgcat
     2641 gccaatctgg atatacggat ttgcatccgc aggaaatgtt ccggtacatg cggtctttac
     2701 cgcactgctg attccaatac tcatcatggc agcttacaca gcgtatcaga cctatactac
     2761 cattgagtcg ttaaagactc tcgcagagac cggaagcatc ggcggcatgc agttctcaat
     2821 gcagtctcca cgataatcct tttttcgttc ttatcactgc tcgtcctttt ctctgcgttt
     2881 cggtaaaata gaaaatgaag attggttgta acaatcagat aattcccaaa ccaaaaatac
     2941 tgcaacagag aaccagatag atcgcttttt caaaaaaaat tatgtgtacc ggttttcgtt
     3001 tttcttcaga cgccagtagc ggtaccctac tgcacagaag atgatgagcc acaaacccat
     3061 acagagtatg aagtactgct cataacttgc gggataaaac cgcatctgaa tcgcacgctt
     3121 atctgtccag acaacaaccg tctcatactt ggctgcatca acagtaattc ccatctcatg
     3181 cagctgatcc atgtcctcgg cggccagata ggttttaccg tcattgctga ccggaccaag
     3241 gatcaggttg ttggtatgaa atcccgcagg aaggaacacc gtagtattga atggcgagag
     3301 atactgcgca tacatcaggc ggccgtcaag cggtgcgtca tagacgagtg tgtagttccc
     3361 ttccggaaag gtgatctgcg tagaactctg cttcgttgca ttcacaatcg tgccgtcctc
     3421 cgcagtcagc gtaaggtttg atacctgaag ctctgcccct tcaccgataa aacccggaga
     3481 gataagcaga tagccgctct ggtttaccag agtgacctct gcggagaagg cagtcccgtt
     3541 ctccgaaata tagtaggatg ccgtgtttgc ggatgcggga accaccagga gtatgcttaa
     3601 cagcagtact gctgcaatta cagcacggaa aggagtgcct cgatggtcgg gtcgatctcg
     3661 attggcgggt tgcattgctt aatcaccgtc tccggatcct ttaagaggtg gccggtaacc
     3721 acacaaacga tcttctcgtt cggatccagc acaccctgct cggcaagttt gcggatacct
     3781 gcaacggatg cggcggatgc gggttcaaca ccaataccct catagcg
//
LOCUS       DALTZZ010000270         2834 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_46494,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000270 DALTZZ010000000
VERSION     DALTZZ010000270.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46494"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(4..600)
                     /locus_tag="O0X12_05220"
     CDS             complement(4..600)
                     /locus_tag="O0X12_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleic acid-binding protein"
                     /protein_id="HJK53584.1"
                     /translation="MSDAGSNFTSYEREPAKRVFASELREATKTFKNTEDEKSPMYVL
                     LPTGERCNRVLVVGTITQKDKVGDQNILYRARVSDPTGLFFVTAGSYQPEAMHQLAKI
                     DPEVPVFVAVVGKPSVYTTPDGKVFTSVRAESVLVVDQETRDLWVQDTARSTLDRVDA
                     LAGDDPSSDVLLARETYRFGPEHWRRMVYDALSRMMTL"
     gene            complement(593..2512)
                     /locus_tag="O0X12_05225"
     CDS             complement(593..2512)
                     /locus_tag="O0X12_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53585.1"
                     /translation="MDQNQIQQITDRISHKLESKGVSPDRQSITDKLSSYINEFGVVP
                     YEAERKVLSDQMRLFDIPEDDVSSAPSSVPEGGVYSLSDIQPGEWVTVEVKVVSLQTP
                     SSPSIAQTGVLADSSGAVRFVVFSKASEIPPLELEQWYRIESAVVDSFREVPSLKLHS
                     GSRVTPITDDRSLMPAPPVSLSELTPGVAASVQVKFVEEWEARSDRILQTGLVADGSG
                     KMKFILWKDEGREKLTLGSVYNIFYASVDTFGGRLSLTLNSGVWMEDEDTEIAVPVVA
                     PAPKGDLPVTSVRDLHVGYVTVQVKFVEEWESRSERMLQTGLLGDETGRIKFVLWKDD
                     TKEKLVPGKVYLIKNAKVAEYNGRLSLSLNSAEYSVVDGADDMVVQGAAPSSPLPVTN
                     IRDLSVGFASLHVKFVEEWESRSERMLQTGLLGDETGRIKFVLWKDDTKEKLEPGRVY
                     HITNAKVDEYNGRLSVVLNSAVYEADDPTADIAVGTVLDSYAGTIVQISGGSGLVKRC
                     PVEGCNRVLSRQNFCPIHEIQNNYNYDLRIKAVLDDGQKAYNVLMGREVTEALTGMTM
                     DQAIDLASESPLGLEEVQAQFTEKLCGRYVRCFGNDFEGRILVKSAEFIHLDPSVTAE
                     LMNRAGSVSGGEINE"
     gene            2629..2814
                     /locus_tag="O0X12_05230"
     CDS             2629..2814
                     /locus_tag="O0X12_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HJK53586.1"
                     /translation="MAITIDSTIADLLREKPESAAILQSFGMGCLGCAIANNETIREA
                     AMVHGIPLEELAKKLGI"
BASE COUNT          743 a          867 c          596 g          628 t
ORIGIN      
        1 caattaaaga gtcatcatcc ggctcagcgc atcgtatacc atacggcgcc agtgttctgg
       61 accaaaccgg tacgtctcgc gggcaagaag aacatcgcta cttggatcat cccccgccag
      121 tgcatcaacc cgatccagag ttgaacgcgc cgtatcctgc acccagagat cacgtgtctc
      181 ctgatccacc acaagaaccg attctgcgcg cacagaagta aacaccttcc catcaggcgt
      241 agtatacaca ctcggcttac caacaacggc aacaaacacc gggacctcag gatcgatctt
      301 tgccagctgg tgcatcgctt caggctgata tgatcccgca gtcacaaaga acagtccggt
      361 tggatccgat acgcgcgcgc ggtagagaat attctgatca ccaaccttat ccttctgagt
      421 aatcgtaccg accaccagaa cacggttgca tctctcaccg gttggtaaaa gaacatacat
      481 cggacttttc tcatcctcag tattcttaaa tgtcttagtg gcctcgcgaa gctctgaagc
      541 aaacacgcgc tttgccggct cgcgttcata cgacgtaaag ttcgaccctg catcactcat
      601 ttatttcacc tccggaaact gaacccgcgc gattcatcaa ctccgcagtc acggaaggat
      661 ccaaatggat gaactcagcg gacttcacca ggatcctgcc ctcgaaatca tttccgaaac
      721 agcgcacata tcggccgcac aacttctccg taaactgtgc ctgtacctcc tcaagaccga
      781 gtgggctctc tgaagcaaga tcaatcgcct gatccatcgt cattcccgta agtgcctctg
      841 taacctcacg ccccatcagc acattgtacg ccttctgccc gtcatccaaa accgccttga
      901 tacggagatc ataattgtag ttattctgaa tctcatgaat cggacagaag ttctggcgcg
      961 acagcacacg gttacaccct tccaccgggc accgcttcac aagacccgac ccgccggaga
     1021 tctgcacaat cgttccagcg taggaatcaa gaacagtccc gactgcaata tctgcagtag
     1081 gatcgtccgc ttcatatacc gcactgttca gaacaacaga aaggcgaccg ttgtactcat
     1141 ccaccttcgc atttgtaata tgatacacgc gtcccggctc gagcttctcc tttgtatcat
     1201 ccttccacag aacgaacttg atccggccgg tctcatcgcc cagcaagccg gtctgaagca
     1261 tacgttcact ccgagactcc cactcctcga caaatttcac atgaagagag gcaaagccta
     1321 cactaagatc ccggatattt gtcaccggca gtggtgatga cggtgcagcc ccctgcacca
     1381 ccatatcatc agcaccatcg acaaccgagt actcagcact gttcagacta agagacagac
     1441 gaccgttgta ctctgcgact tttgcatttt tgatcagata cactttgccc ggtacaagct
     1501 tctcctttgt atcgtccttc cacagaacga acttgatccg accggtctca tcgcccagca
     1561 agccggtctg aagcatacgt tcactccgag actcccactc ctcaacaaac ttcacctgga
     1621 ctgtcacata accaacatga agatctcgaa cacttgttac cgggagatca ccttttggag
     1681 caggggccac aaccggtact gcaatctccg tgtcctcatc ctccatccac acaccggaat
     1741 tcaacgtaag cgacagacgt ccgccgaacg tatccacgct cgcatagaat atattataga
     1801 ccgaaccaag ggtcagtttc tcccgaccct catccttcca cagaatgaac ttcatcttcc
     1861 ccgacccatc cgccacaagg ccagtctgaa gaatgcgatc gctccgcgct tcccattcct
     1921 caacaaactt cacctgaaca gaggctgcaa caccgggagt tagttcactc agggaaacgg
     1981 gaggagccgg cataagactc cggtcgtcgg tgatcggtgt aacacgggag cccgagtgaa
     2041 gtttcagact tggaacctcg cggaacgaat caacaaccgc cgactcaatc cggtaccact
     2101 gttcaagttc cagcggagga atctcactcg cttttgaaaa caccacaaac cgtaccgcac
     2161 cggagctgtc agcaagaaca ccggtctgtg caattgacgg ggaactcggt gtctgcaaag
     2221 acaccacctt cacctcgaca gtgacccact cacccggctg aatatcggaa agagaataga
     2281 caccgccttc cggcaccgag gacggcgcgg aggatacatc atcctcagga atatcaaaca
     2341 gccgcatctg atcggacaaa acctttcgtt cagcctcata cggcaccaca ccgaactcat
     2401 tgatgtagct tgagagttta tccgtaatgc tctgtctgtc cggcgaaact cctttggatt
     2461 ctaatttatg ggagattctg tcggtaatct gttggatctg attttggtcc atattgatct
     2521 ccaacagatt cattggcttc acactatatt aacccccgac catgcgtact gaaagtgccg
     2581 gtgccctgtt cggaaacatc aagtagtcga attgccaatg tagtatacat ggcaattacc
     2641 atcgattcaa ctatcgccga tctgctccgc gaaaagccgg aaagtgccgc aattctccag
     2701 agcttcggca tgggatgcct tggctgtgca atcgcaaaca acgagaccat ccgcgaggcc
     2761 gcaatggttc acggaatccc cttagaagag cttgccaaaa agctcggcat ctaaaaatat
     2821 tttatttttc attc
//
LOCUS       DALTZZ010000271         3508 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.5 k141_46779,
            whole genome shotgun sequence.
ACCESSION   DALTZZ010000271 DALTZZ010000000
VERSION     DALTZZ010000271.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062331
            Sequence Read Archive: ERR2193605
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 0.762615x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:50:34
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,050
            CDSs (total)                      :: 1,019
            Genes (coding)                    :: 1,012
            CDSs (with protein)               :: 1,012
            Genes (RNA)                       :: 31
            rRNAs                             :: 2 (5S)
            complete rRNAs                    :: 2 (5S)
            tRNAs                             :: 27
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46779"
                     /isolate="HAC023.5"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            56..616
                     /locus_tag="O0X12_05235"
     CDS             56..616
                     /locus_tag="O0X12_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HJK53587.1"
                     /translation="MFTSLLSTFFIAVGLAMDAFSVSLAGGAVLKKDVFRTALVAGLL
                     FGFFQFAMPLIGYVIGVPITNLIMPYGYWLVFFLFLFVGGKMIYDAVFGSEECGIDLT
                     GWKVLLLLAVATSIDALAVGVSYALLGEEILLPAVIIGVVAFGFSAAGVFVGNRLGCV
                     LGNKMEILGGVILVLIGCKFLLENVL"
     gene            complement(671..1753)
                     /locus_tag="O0X12_05240"
     CDS             complement(671..1753)
                     /locus_tag="O0X12_05240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53588.1"
                     /translation="MDHRYLLLILVVLFLAVPVTAVEYDNTLTVSFDGYADSSSAESY
                     ADGSTVVIPYGSTVWIGATQYPGGKWTVTVPNLYPVKPSGKEIIQLSFGNVQGLYSIT
                     YDVPSVYYTNGAPKTITLNLNIGDVGGDSYADSIYAKYPNRYTLIFSDEYPVLQEGNT
                     KGLFSFGDPYNAPYIRVISVTTNGSEISQNARIVLVPQSSANPIPTPSQPYTALYDMK
                     VENIPVDAIEQVDFLFGVPVGVVTDAGFDPRYDIQLLRYTDSWEKLETSYVGEGTGQQ
                     AGMDWYAAKSPGFSYYAVGFAENVTKLPEEIFTPTPTATVEVITPPPTTATTEPSVPT
                     APEQSSPFVFTGIIALLGAAVLCLRR"
     gene            complement(1780..2277)
                     /locus_tag="O0X12_05245"
     CDS             complement(1780..2277)
                     /locus_tag="O0X12_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK53589.1"
                     /translation="MKIPVAAGVLAVLVLITFSAGCIGDQTTDIMLGNQSIGSITVTP
                     VYENMFSNSSLGDKFNVKLEIFGMVFTKDNVTRAEADNISASILDKDGGVNMSFINSS
                     GLITPNANDAESNLTSFMDEVFNMPLTNRDKNTTINDINWDDAFSRMQESFNRLTGLL
                     NQSGS"
     gene            complement(2348..>3508)
                     /locus_tag="O0X12_05250"
     CDS             complement(2348..>3508)
                     /locus_tag="O0X12_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HJK53590.1"
                     /translation="DATEEELRAAVAAAGASFIGYENGVGSTTAETVKRILESKVNSL
                     GTNDVKVNTLTNTNGVSQYVRVEMAGVDMQTAQDIVGKQGLFEIRIVTTGNETAHVLY
                     GDSVSSVQNPTQNPAGSDNWGVGFNLDNDGAVALQQACIQYGATTNPQAHNLNMYLDG
                     VEVYSAPLSNDLAMQIATHPVNSLYAGTGSGEEGYQQAQELEIHLRAGALPVQVEIAG
                     SGSTSAALGDYFKIVCLIAGLAALAAVAAIVYLRYRVAEIVLPMIATNMAEIIILLGI
                     AVFIQQLDIAAIAALIAVLGTGIDQLVIITDEVVHEGKVPSQTLYLKRLTRALAIIMT
                     SAATVIIAMFPLIVMDLSTLKGFAIISILGILIGVLITRPAYGKIVMDIMAK"
BASE COUNT          802 a          828 c          917 g          961 t
ORIGIN      
        1 ccggaaggca tggacaatgt gagaaagaac ggaaaaccgg tctggtagtt ttttcatgtt
       61 cacctcactg ctttccactt ttttcatcgc tgtcggtctt gcaatggacg cattctccgt
      121 ttcgcttgcg ggaggtgcgg tgctgaaaaa agatgtcttc agaacagcac tggttgcagg
      181 tctgctgttt ggttttttcc agtttgccat gccgctgatc ggctatgtga tcggtgttcc
      241 gatcactaat ctcatcatgc cgtacgggta ctggcttgtc tttttcctgt tcctgtttgt
      301 cgggggcaag atgatctatg acgcggtttt tggtagtgag gagtgcggga tcgatctgac
      361 cggctggaag gttctccttc ttcttgcggt tgctacaagt atcgatgccc ttgccgttgg
      421 tgtgagttat gctcttcttg gagaagagat tcttcttcct gctgttatca tcggtgtggt
      481 cgcttttggt ttttctgcgg caggtgtttt tgttggcaac agactcggct gtgtgcttgg
      541 aaacaaaatg gaaattcttg gcggagtcat tcttgttttg atcggttgca agtttctgct
      601 ggaaaatgtt ttgtgaactg gcagacccta tttttcgtaa tatggatttt aaaaaagaag
      661 actggggatt tcaccttcgc aggcacagca ctgcagctcc cagcagggcg atgatacctg
      721 taaatacaaa gggtgaggac tgttcgggcg cggtcggaac agaaggttct gtggtcgccg
      781 tggttggagg cggtgttatg acctcaacgg ttgctgtggg agtcggcgta aagatctcct
      841 ccgggagctt cgttacgttt tcggcaaatc ctactgcata gtaggagaat cccggtgatt
      901 ttgcggcata ccagtccatg ccggcctgct gcccagttcc ttccccgaca tatgacgttt
      961 ccagtttttc ccacgaatcg gtgtaacgga gcagctggat gtcgtaccgc ggatcaaatc
     1021 cagcatcggt taccacaccg accggaacac caaagaggaa atccacctgc tcaattgcat
     1081 caaccggaat attctcgacc ttcatgtcgt agagtgcggt gtacggctgg gatggtgttg
     1141 ggatcgggtt tgcggagctc tgcggaacga gtacaattct tgcgttctgt gaaatctcag
     1201 atccatttgt cgttaccgag atcacccgga tgtacggggc attataggga tcgccgaaac
     1261 tgaagagtcc tttggtattt ccttcctgga gaaccgggta ctcatcggaa aagatcagcg
     1321 tatagcggtt cggatacttt gcgtagatgg agtcggcata tgaatctccg ccgacatcgc
     1381 ctatgttgag gttcagagtg atggtctttg gtgcaccgtt tgtgtaatag acggacggca
     1441 cgtcgtaggt gatggagtat aatccctgaa cattaccaaa actcagctgg atgatctctt
     1501 tccctgatgg ctttaccggg tagaggtttg gaacggtaac cgtccacttt cctccagggt
     1561 actgggttgc cccaatccag acggtacttc catagggaat aacaacagtc gatccgtctg
     1621 cataactttc agcggaagag gagtcggcat atccgtcaaa agatacggtt agtgtattgt
     1681 catactccac tgctgttacc ggtacggcaa gaaacaggac tactaaaatg aggagaagat
     1741 atcgatgatc catagtagtc catgtgtgat tgaattatat taacttcctg attggttcaa
     1801 taatccggtc agacggttaa aagactcctg catgcgggag aatgcatcat cccagttgat
     1861 atcgttgata gtggtgtttt tgtcccggtt ggtcagcggc atgttgaata cttcatccat
     1921 aaaggaggtg agattcgact ctgcgtcatt ggcattcggg gttatcaggc cggaactgtt
     1981 gataaacgac atgtttaccc cgccgtcttt atccagaata ctggcactga tgttatcggc
     2041 ttcggcccgg gtgacattat ccttggtaaa taccatccca aatatctcca gctttacatt
     2101 gaacttatcc ccgagtgagc tgttggaaaa catattttcg tacaccggtg ttacggtgat
     2161 cgaacctata ctctgattgc caagcatgat atcggtggtc tgatccccga tacatcctgc
     2221 ggaaaatgtg atgagaacaa gaactgcaag aactcctgcg gcaaccggaa ttttcataga
     2281 aatgtgtttg tgccgactgg aaataaatat ttgcggatat ccgctgaaaa ataaatttaa
     2341 aaatttttta tttggccatg atgtccatca caatcttacc ataggctggt ctcgtgataa
     2401 gaacaccaat caggatacca agaatactga tgatggcaaa acccttgagg gttgagagat
     2461 ccatcacaat cagcgggaac attgcaatga tcaccgttgc tgcggatgtc atgataatgg
     2521 caagtgcccg cgtaagccgc ttgagataca gtgtctgcga gggaaccttt ccttcatgca
     2581 ccacttcatc ggtgatgatc accagctgat cgataccggt tccaagaacc gcgatcaatg
     2641 ctgcgattgc tgcaatgtcg agctgctgga tgaagacagc aataccgagc aggatgatga
     2701 tctccgccat gttggttgcg atcatcggca gcacaatctc ggcaacccgg taccggagat
     2761 agactattgc cgccactgcc gcaagggcag caagtcctgc aatcagacag acgatcttaa
     2821 agtagtcacc aagtgccgca gaggttgaac cggatccggc aatctcaacc tgcactggca
     2881 gagcacctgc ccgcaggtgg atctccagtt cctgtgcctg ctggtatcct tcctcaccgg
     2941 aaccagttcc tgcatacaga ctgttgaccg gatgtgttgc aatctgcatt gcaagatcat
     3001 tggagagcgg agcactgtat acctcaaccc catccagata catgttcagg ttatgtgcct
     3061 gcgggttggt ggttgctcca tactggatac aggcctgctg cagtgccact gctccgtcgt
     3121 tgtccaggtt gaatccaaca ccccagttgt cggaacctgc cgggttctgt gtgggattct
     3181 gaacagatga taccgagtca ccgtacagca catgggctgt ttcgttgccg gttgttacga
     3241 tccgaatctc aaagagaccc tgttttccaa cgatgtcctg agcggtctgc atgtcaacgc
     3301 ctgccatctc aacacggaca tactgtgaaa ctccgttggt gttggtcagg gtgttgacct
     3361 tgacgtcatt ggttccaaga ctgtttacct tggactccag aatccgtttg acggtctcgg
     3421 cggttgtaga tcccactccg ttttcgtatc cgatgaagct cgcaccggct gctgcgaccg
     3481 cggcccggag ttcttcttcg gttgcatc
//
