LOCUS       DALUAJ010000001         3593 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_227,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000001 DALUAJ010000000
VERSION     DALUAJ010000001.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="O0X37_00005"
     CDS             complement(<1..206)
                     /locus_tag="O0X37_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61342.1"
                     /translation="MSASEIAALLSNAEITPQYIRHAVHHLTKPERSILYDAAHPLHR
                     SVSGKYFEAVVYELLLGCAEHSDA"
     gene            complement(214..1896)
                     /locus_tag="O0X37_00010"
     CDS             complement(214..1896)
                     /locus_tag="O0X37_00010"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HJK61343.1"
                     /translation="MSDTNIRDDIYLFALQNAVKHKSVPKAGAILGSVMGAHPELRPQ
                     AKEINAMLPEILAEVEVMSVEEREAKLTALSPEMIEKMHEKKERVHELPELPEAGNGV
                     VMRFAPNPSGPLHLGHARASVLNDYYVKKYGGKFYYRVEDTDPKRVDPEAYDMVMEDL
                     KWLGIGITDVVYQSDRFQIYYDLARELIKLGGAYMCTCDNDEFREKKLKKQPCPCRDL
                     SVEENLRRFDDMLAGKYQEGEITMRVKTDIAHPDPAVRDFAAMRVLTSVKHPRRPEIF
                     VYPLMNFSVAVDDHLLGMTHVIRGKDHIANTRRQEYIYRYFGWKMPHFYHYGRMSIAG
                     LELSASGMRKGINEGLYTGWDDIHLGTLRTLARRGIQPEAVRAAVVDIGMGDTDISFS
                     WENLFAQNKAIIDAEADRYFFVPDAVPVTVANAPAMEAHAPIYPNHPERGERVLSFTG
                     SVLLPRSEIETGRMLRLKDLFNIRITGEGSAEYAGDSLAEARAAKAPIVQWLPTDAGV
                     PCSLLTPEGTSEGYCEVAVLNYDQKIVQFERVGFARIDSVKDGHVTAYFTHR"
     gene            complement(1978..2592)
                     /locus_tag="O0X37_00015"
     CDS             complement(1978..2592)
                     /locus_tag="O0X37_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61344.1"
                     /translation="MDLLERRVDLDDVREKYRRKNQLLFSRESACLQDLIRLICLQKH
                     RTIVLWAFTCAENPVALLKRAYPDDTRPEEAVRVCRMWAEGCVKMPEARKAILQVHAM
                     AREVETPRDCALCHAVGQACSAVHTEAHAVGLVFYELTAIVRELGIDACEQAVDEKIN
                     YYISTLKDCQAEIDFQNRSWAAFLLDDSRVNKELLLWQKRSKLP"
     gene            complement(2611..3264)
                     /locus_tag="O0X37_00020"
     CDS             complement(2611..3264)
                     /locus_tag="O0X37_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HJK61345.1"
                     /translation="MTPADVSSIVRNHVRSALLGEATGHDWWHVFRVTGLAAKISETE
                     GGDPLRVELTALLHDLFDEKLYGGDPGEALSACIDQLGIREAFPDAVWDGIIFDICHL
                     GFKGGFDQTPLSLEGQIVQDADRLDAMGAIGIARAFAYGGAKGRELYNPDEGIVTVTT
                     AAEYRTGSRHTVNHFYEKLLLLRDRMNTNAAKTLADQRHQFMEEYLAEFFAEWESAV"
     gene            complement(3345..>3593)
                     /locus_tag="O0X37_00025"
     CDS             complement(3345..>3593)
                     /locus_tag="O0X37_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HJK61346.1"
                     /translation="ILAITAREMGVDLSKYHKENLTKEEIAEAIALLEESGVLGKVRS
                     ISEKLIADSKAGLANVVPASEERDLIFEMVDFFITRGY"
BASE COUNT          905 a          964 c          897 g          827 t
ORIGIN      
        1 atcgcatccg agtgctccgc acatccaagc agcagttcat agacaactgc ttcaaaatat
       61 tttccgctga cagagcggtg cagagggtgt gccgcatcat acaggataga acgttcaggt
      121 tttgtcagat gatgaacggc atgccggata tactgcggcg taatctctgc attggataga
      181 agtgcggcaa tctcggatgc ggacatgaaa aaatcagcgg tgggtgaagt aggcagtgac
      241 atgcccatcc ttcaccgagt cgattctggc aaacccgaca cgctcaaact gcacgatctt
      301 ctgatcatag ttcagcacag caacctcgca gtacccttca ctcgtacctt ccggggtcag
      361 aagcgagcag ggaacacccg catccgttgg aagccattga acaatcggtg cctttgcagc
      421 ccgtgcctcg gcaagagagt ctccggcata ctcggcactg ccctcgcccg taattctgat
      481 attgaagaga tctttgagac gaagcattct tccggtttca atctcggatc tcggcagaag
      541 aacactgccc gtaaacgaga ggacacgttc tccgcgttcc ggatgattcg ggtaaatggg
      601 ggcatgcgct tccattgcag gagcatttgc aacggtaacc ggaaccgcgt ccggaacaaa
      661 gaagtatctg tcagcctctg catcgatgat tgctttgttc tgggcaaaca ggttttccca
      721 cgaaaacgag atgtctgtgt cgcccattcc aatatcaaca accgcagccc gtactgcttc
      781 cggctgaata ccgcggcggg caagagtacg aagcgttccg agatgaatat catcccaacc
      841 ggtgtagaga ccctcattga tccccttgcg cataccggag gcggaaagct caagtcctgc
      901 gatggacatt ctgccatagt ggtagaagtg cggcatcttc cagccgaagt accggtagat
      961 gtactcctga cggcgggtat tggcgatgtg atccttgccg cggataacat gcgtcatacc
     1021 aaggaggtga tcatcaacgg caacggagaa gttcatcagc ggatagacaa aaatttcggg
     1081 acggcgggga tgcttgacgg aggtgagaac acgcattgcg gcaaaatcac gaacagccgg
     1141 gtcagggtga gcgatatcgg ttttgacccg catggtgatc tctccctcct gatattttcc
     1201 ggcaagcatg tcgtcgaacc ggcggagatt ttcctccacg ctgaggtcgc ggcagggaca
     1261 cggctgcttc ttcagctttt tctcacggaa ctcatcgttg tcacaggtac acatgtaggc
     1321 accgccaagt ttgattaact cgcgtgcaag atcgtagtag atctggaacc ggtcggactg
     1381 atagaccaca tccgtaatgc cgataccgag ccacttgaga tcctccatga ccatgtcgta
     1441 ggcttcagga tcaacacgct ttggatcggt gtcctcgaca cggtagtaga atttgccgcc
     1501 gtactttttg acatagtaat cattgagaac cgacgcacgg gcatgaccga ggtgaagcgg
     1561 acccgacggg tttggggcaa accgcatgac gaccccgtta ccagcttcgg gaagttcagg
     1621 gagttcatgc acgcgctcct tcttctcgtg catcttctcg atcatctccg gtgaaagcgc
     1681 tgtgagtttt gcttcgcgct cctcaacaga cattacctca acctctgcaa ggatttcggg
     1741 aagcatcgca ttgatctctt ttgcctgcgg acgaagctcg ggatgggcgc ccataaccga
     1801 gccgagaatg gcaccagcct taggaacgga cttgtgcttt actgcgtttt gcagtgcgaa
     1861 aagatagata tcatcacgaa tatttgtatc ggacatttgc gggagttata ttggcgggag
     1921 aggtgtttat actttcacaa caaaaaattc acagcggaaa aaattgtggc tgtaccctca
     1981 cgggagtttt gatctttttt gccagaggag aagttccttg ttgactctgc tgtcatccag
     2041 aagaaaagca gcccacgaac ggttttggaa gtcgatttct gcctgacagt ctttgagcgt
     2101 ggagatatag tagttgatct tctcatccac agcctgctca catgcatcaa tgccaagttc
     2161 gcggacaatg gcggtcaact cataaaagac cagcccgact gcatgggctt ccgtatgtac
     2221 agcagaacat gcctgtccta ccgcatggca caacgcgcaa tcccgcgggg tttcaacctc
     2281 acgcgccatg gcatgaacct gaagtatcgc ttttcgggcc tcaggcatct tcacacatcc
     2341 ctcagcccac atccggcata cccggacagc ctcttccggt ctggtatcat cgggatacgc
     2401 tcttttcaaa agagcgaccg gattttcggc acatgtaaac gcccacagaa cgatcgtccg
     2461 gtgtttttgc agacagatca gacggataag atcctgcagg caggcactct cgcgggaaaa
     2521 cagaagctgg ttttttcgcc ggtacttctc gcgtacatca tccagatcaa ctcttcgttc
     2581 caacaaatcc atccatatca cctgaaaagg tcaaaccgca gactcccact ccgcaaaaaa
     2641 ctcggcgaga tattcctcca taaattggtg gcgctgatct gcaagtgttt ttgcggcatt
     2701 ggtgttcatt ctatcacgca gaagcaggag tttctcgtag aaatgattga cagtatgacg
     2761 actgccggta cggtactccg cagcagtggt aaccgtcacg attccttcat cgggattgta
     2821 cagctcgcga ccttttgccc cgccgtaagc aaatgcccgt gcaatcccga tagcacccat
     2881 cgcatcaaga cggtcggcgt cctgtacaat ctgtccttcc agtgacagcg gtgtttgatc
     2941 aaatcctccc ttaaagccga ggtggcaaat atcaaagatg atgccgtccc agactgcatc
     3001 aggaaacgct tcacgaatac caagctgatc gatacaggca gacaatgctt ctccgggatc
     3061 accaccatag agcttttcat caaaaagatc gtgcagcagg gcggtcagct caacccgcag
     3121 aggatctcct ccttcggtct cgctgatctt tgcagcgaga ccggtaacgc ggaacacatg
     3181 ccaccagtca tgaccggttg cctctccaag cagggccgaa cgaacatgat tacgaacaat
     3241 ggaggaaaca tctgccggag tcattcgcaa ggatattacc gctaaaacaa tatgaactca
     3301 gaggagagga ctgcaccgaa aaagtactga accaaaaaaa gagattagta tcctctggtg
     3361 ataaagaaat caaccatctc aaagatcaga tcccgctcct cggatgcagg aaccacattt
     3421 gcaagacctg cctttgaatc cgcgatcagt ttttcggaga tgctgcggac tttcccgagg
     3481 actcccgact cttcaagaag tgcaattgcc tctgcaatct cctcttttgt aagattctct
     3541 ttgtgatact tggaaagatc cacgcccatc tcgcgggcag taatggccag aat
//
LOCUS       DALUAJ010000002         1623 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_603,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000002 DALUAJ010000000
VERSION     DALUAJ010000002.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_603"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="O0X37_00030"
     CDS             <1..551
                     /locus_tag="O0X37_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HJK61347.1"
                     /translation="IVDLSLAPTPAIGDSVAEILEEMGLESVGAPGTTAALALLNDQV
                     KKGGVMASSFVGGLSGAFIPVSEDQGMIDAVNRGALTLEKLEAMTCVCSVGLDMIAVP
                     GSTPATTIAGIIADEAAIGMINQKTTAVRLIPVIGKDIGDTVEFGGLLGYAPIQPVNR
                     FSCAAFVNRGGRIPAPIHSFKN"
     gene            complement(595..1023)
                     /locus_tag="O0X37_00035"
     CDS             complement(595..1023)
                     /locus_tag="O0X37_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61348.1"
                     /translation="MGSDYTPERVFYGNHADPPLMKDFLSAAMYRTADRLLRDGGVPD
                     TAQNGSYGQKLYYCPQCRTLKVGFFCTIIHDDRCWTPQYTCDLCQHPLVLVPNQENGD
                     DPSRDQMAGPDGAVLRICCKKCGRVYFAPESGCTSVFLWD"
     gene            complement(1329..>1623)
                     /locus_tag="O0X37_00040"
     CDS             complement(1329..>1623)
                     /locus_tag="O0X37_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HJK61349.1"
                     /translation="ADEARFRGVFDVLCRNPDLWDMAILVNFPNKVLSPEQVARVLID
                     YSKKTENLLVGTLVGGDCMKPGVSLLTQHNIPVFEELEFTYRTLGHLSWTADR"
BASE COUNT          443 a          406 c          402 g          372 t
ORIGIN      
        1 gtatcgtgga tctttctctt gcaccaacac ctgcaatcgg cgacagtgtc gcagaaattc
       61 ttgaagagat gggacttgag tcggtaggag ctcccggaac gaccgcagca cttgcacttc
      121 tgaacgatca ggtgaaaaag ggaggagtga tggcaagttc gttcgtcgga ggactttccg
      181 gggcatttat tccggtcagc gaggatcagg gcatgatcga tgcggtcaac cgcggtgcac
      241 tgacactgga aaaacttgag gcaatgacct gtgtctgttc agtaggcctt gacatgattg
      301 cagttccggg tagcactcct gcaacaacga ttgcaggaat catcgcagat gaggcagcaa
      361 ttgggatgat caatcagaaa acaacagctg ttcgtctgat cccggtcatc ggaaaagata
      421 ttggtgatac ggtggagttc ggcggtcttc tcggctatgc accaattcag ccggtcaacc
      481 gtttctcctg tgcggcgttc gtgaaccgcg gcggaagaat tcctgcaccg attcacagct
      541 tcaagaatta atttcttttt ttaccctggt tttctctgaa aagatcctac agaactaatc
      601 ccagagaaac acggatgtac agccgctttc cggtgcaaag tatacgcgtc cgcatttctt
      661 gcagcagatt cgaagaacgg caccatcagg cccggccatc tgatcccggg acggatcatc
      721 accgttctcc tgatttggca caagtaccag aggatgttgg cagagatcac aggtatattg
      781 tggtgtccag caccgatcat cgtgtataat tgtacagaaa aaacccactt tcagggttct
      841 gcactgcggg cagtagtaaa gtttctgacc ataactcccg ttctgtgcag tatccgggac
      901 accgccgtcc cgaagcagac gatccgcagt acggtacatt gccgcggaaa gaaaatcctt
      961 catcagcggc ggatctgcat gattcccgta aaagaccctc tctggtgtgt agtcagaacc
     1021 catacctaga gcaatatatg tcgcctctcc acagtcagga caatgataaa cataccccga
     1081 acccatacac ctcacctgtg gtatcatatt gattagcaga tgtagtaaaa tggttagata
     1141 gtaccactaa attgttgcag acccaaacag atcaacggta aaatatctgt actgtccaga
     1201 tgaaacgctg agatatgaga aaaaacaaac ggcacaaaaa ctcgctctac agctttcagg
     1261 aatactaaca caaaaaaaga aggttccagg gtttcggagg taaaacccgg atatttggca
     1321 caggggggtt aacggtccgc agtccagctt aagtgaccaa gtgttctgta agtaaactcc
     1381 agttcctcaa acaccgggat attgtgctgg gtaagaagcg atacaccggg tttcatgcag
     1441 tctccgccca ccagagttcc aacgaggagg ttctccgtct tcttcgagta atcgatcaga
     1501 acacgggcaa cctgctcggg gcttaacacc ttgttcggga agttgacaag aattgccatg
     1561 tcccagagat ccgggttacg gcagagcacg tcaaacacac cccggaaccg agcttcatcc
     1621 gca
//
LOCUS       DALUAJ010000003         2190 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_758,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000003 DALUAJ010000000
VERSION     DALUAJ010000003.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="O0X37_00045"
     CDS             complement(<1..366)
                     /locus_tag="O0X37_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HJK61350.1"
                     /translation="MTVARFWRAIPQRYNMVGTKCETCGRVYFPPRSVCPHCRRNGKI
                     VEHKCSGKGKVLTFSVVHSASDQYADMKPYVLAIVELEEGARMTTQIVCEPEEVYIGM
                     PVKSVFRVLDREGSDGVIHY"
     gene            complement(369..1532)
                     /locus_tag="O0X37_00050"
     CDS             complement(369..1532)
                     /locus_tag="O0X37_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HJK61351.1"
                     /translation="MREVAVIGAGITAFGERWDTSFRDLCTEAGVAALEDANVAGEQI
                     DAMFVGSMSAGRFIGQEHVGALVADYVGLGGELHVPATRVEAACASGGLAFRQAVAAV
                     ASGMSDVVVAAGVEKMTDVSDATDVLAGAADREWESFAGATFPGLYAMIACDYMNKYG
                     LTREQLAQVAVKNHYHGARNPYAQFRSEITQSTVIKSTLVASPLRLFDCSPVSDGAAV
                     VIVCPMERAKEFTDTPIKVLASAQAGDTIALHDRPDFSTLGATVAAGNAAFKQAKLER
                     KDIDFVEVHDCFTIAELCAIEDLGFVPKGTAGKFTEEGETQIGGKLPVNTSGGLKACG
                     HPVGATGIKQVWEVVQQLRGEAGKRQVDGAEIGMTQNVGGTGATVVSHIFRRA"
     gene            complement(1542..>2190)
                     /locus_tag="O0X37_00055"
     CDS             complement(1542..>2190)
                     /locus_tag="O0X37_00055"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HJK61352.1"
                     /translation="YAVAIGADTAQGAPGDALEYTAAAGGAAYVIGNDDPIAVINHTC
                     SFSTDTPDFWRREGEDYPRHGGRFSGEPGYFKHVTGAARMMLEHMGTKPADYDYAVFH
                     QPNAKFPQKGAAMLGFTHDQIKTGLLAPRLGNTYSGAVPLGLAAVLDIAKPGDRIFIT
                     SYGSGSGSDAFDLTVTDAILDKIDRTKGPSVEAMLAAGKYLDYAVYARHKGKIKV"
BASE COUNT          493 a          631 c          519 g          547 t
ORIGIN      
        1 atagtggatg acaccgtctg atccttcacg gtccagaacg cggaataccg atttgaccgg
       61 catgccgatg tacacttctt cgggctcaca gactatctgt gtcgtcatac gtgccccttc
      121 ttccagttca acaatcgcca gaacatacgg tttcatgtcc gcatactgat cacttgccga
      181 atggacaact gaaaaggtca ggacttttcc tttgccggaa catttgtgtt cgacaatctt
      241 tccattcctg cggcagtgag ggcagaccga acgcggcggg aaatatactc tgccgcaggt
      301 ctcacacttc gtaccgacca tattgtatct ctggggaatt gctctccaga atcgtgcaac
      361 tgtcattgtt aggccctcct gaaaatatgt gacacaacgg tagcaccagt accacccacg
      421 ttctgggtca taccaatctc tgctccatcg acctggcgtt tacctgcttc acctctcagc
      481 tgctggacaa cctcccagac ctgcttgatg ccggttgcac ctactgggtg accgcatgct
      541 ttcagacctc cggacgtatt cactggcagt tttccaccga tctgtgtttc tccttcttcg
      601 gtgaactttc ctgctgttcc cttcggaaca aatccgaggt cctcaatggc acagagctct
      661 gcgatcgtga aacagtcatg aacctcaaca aagtcaatat ctttgcgctc cagttttgcc
      721 tgcttaaatg cggcgttacc tgcggcaact gttgcaccca gggtggaaaa gtcggggcgg
      781 tcatgaagtg caattgtatc tcctgcctga gcggaggcaa gcaccttaat cggcgtgtcg
      841 gtaaactcct ttgcgcgttc catcgggcag acaatcacta ctgctgcacc gtctgaaacc
      901 ggggaacagt cgaaaagccg cagcggtgat gcaacaagcg tggattttat gactgttgac
      961 tgtgtgatct ctgatctgaa ctgtgcatac ggattacgtg cgccgtggta gtggttcttt
     1021 accgcaacct gtgccagctg ttcacgggta aggccgtact tgttcatgta gtcacaggcg
     1081 atcattgcat aaagacccgg gaatgttgct cctgcaaaac tctcccactc acggtcggct
     1141 gctccggcga ggacatcggt tgcatccgaa acatccgtca tcttctcaac acctgcggcg
     1201 acaaccacat cggacatgcc ggacgctact gctgcaactg cctggcggaa tgcaagacct
     1261 ccggaagcac atgctgcttc cacacgggtt gccggtacat ggagttctcc accgagacct
     1321 acgtaatcgg caacaagagc acctacatgt tcctggccaa taaatcttcc agcagacatc
     1381 gatccgacaa acattgcatc gatctgttcg cctgcaacat ttgcatcctc aagggctgca
     1441 acacctgctt ccgtacacag atctcggaac gaggtgtccc aacgctctcc aaatgccgta
     1501 attccggcac caataactgc tacttctctc atttttgcac ctcacacctt gatctttcct
     1561 ttgtggcgtg cgtacaccgc ataatcaaga tactttcctg cggcaagcat tgcctcaacg
     1621 ctcggtcctt tggttctgtc gatcttgtca agaatcgcat ccgttacagt cagatcaaat
     1681 gcatctgatc ctgaaccgct gccgtaactt gttatgaaaa tacgatctcc gggttttgca
     1741 atatcaagta ctgctgcaag gccaagcggt actgcaccac tgtaggtgtt tccaagtctt
     1801 ggagcaagaa gtccggtctt gatctgatca tgtgtgaaac caagcatcgc agcacctttc
     1861 tgagggaact ttgcgtttgg ctggtggaac acagcataat cgtagtccgc gggctttgta
     1921 cccatgtgtt ccaacatcat gcgtgcagcc ccggttacgt gcttgaagta tccaggttca
     1981 ccggagaacc gtccaccgtg gcgggggtaa tcctcacctt cacggcgcca gaagtcaggc
     2041 gtatcggttg aaaatgaaca ggtgtggtta atcactgcaa ttggatcgtc gtttccaatc
     2101 acgtatgctg cgccgccggc agctgcagtg tactccagag catcgccggg agcaccctgt
     2161 gccgtatccg caccgatcgc aaccgcatac
//
LOCUS       DALUAJ010000004         2513 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_761,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000004 DALUAJ010000000
VERSION     DALUAJ010000004.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            69..431
                     /locus_tag="O0X37_00060"
     CDS             69..431
                     /locus_tag="O0X37_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61353.1"
                     /translation="MRAKFSRARQISPAMVGTPVIVEGKILKGTGLLMGKPAYLLSDS
                     TGQIVVRRFALPDPLVGVGAAVEVVGTVARKITNGDAVYVNAVSIKPIRKFREEGSKE
                     SGEKPSAGDKKIHIKRLH"
     gene            1004..2077
                     /locus_tag="O0X37_00065"
     CDS             1004..2077
                     /locus_tag="O0X37_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HJK61354.1"
                     /translation="MKLIHCADIHLDSPMETHMTTEQASMRNTEIIHSFIRMTEYAKK
                     NEVRVVIIAGDLFDGERIRTRTVDEILDAIRSTPSVDYLYLAGNHDESSHAFSNHDLP
                     QNLKQFNRKWNSYVYEDVMISGIEICDENATSLYLNIPHRDGCVNIISLHGQVGTTCG
                     IDQINLNLLKNQGIDYLALGHLHSYSVHSLDNKGIYCYSGCLEGRGFDECDKKGFVLL
                     TVESGRLQHEFVPFCCRQLHQVDVDITGLTKNSEIGQMMKSAVQGIQPDDMVEFILTG
                     YSDPTANIAVSYLQNLINNSFFFTKVKNESRMAINPEDYKNDISLKGEFIRLVLDSDA
                     SDEDKAAIIRTGIQALTGEEIGL"
     gene            2074..>2513
                     /locus_tag="O0X37_00070"
     CDS             2074..>2513
                     /locus_tag="O0X37_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJK61355.1"
                     /translation="MKLLRCHIENFGVLSNFDFDFSSGLTVIYRENGFGKSTFAAFIK
                     AMFYGMPRGGSRSVVENERKRYDPWQGGKYGGFLEFEYQETRYRVTRYFGKTASKDTF
                     QIMDISNRQNITPFSERLGEELFLLDAESFSRSTYMPQLSAHDL"
BASE COUNT          710 a          547 c          577 g          679 t
ORIGIN      
        1 tctgtgtccc gcttgcaatg aactatctga ccgaaaagca gcttgcctag cagctgcctg
       61 cgatgcgggt gcgggcgaag ttttcccggg cacgccagat ctctcctgcg atggtcggga
      121 caccggtgat tgtggagggg aagattctga aggggacggg acttttgatg ggaaagcctg
      181 cgtatctgct ctcggactcc accggccaga tcgttgtccg ccggttcgct ctgccggatc
      241 cgctggtcgg ggttggtgcc gcggttgagg ttgtcgggac ggttgcacgc aagattacca
      301 acggcgatgc ggtctatgta aatgcggtat ccatcaagcc tatcagaaaa ttccgggagg
      361 agggttcaaa agagagcggg gaaaagccgt ctgccggaga caagaagatc catatcaaaa
      421 gactccatta accaggatta accaggactg cggaaacatc acactgacga cgaataatga
      481 acgccactag atttcccgtg atgcggcgaa ttcctcttcc aattaccagt attttcccca
      541 ttaatggtgg gaaccagaca attactggaa cgatacctac accacttttg ctgatcttat
      601 gcaggacaga aactaaacct cagtaaccag caacacgcag tccctagtgg atgtgaccaa
      661 cagcggcaga ttcacgcttg agcaccgttg gatcctcacc atcaatacgg caagagggaa
      721 cggaggcggt gtgtacgtga acagtacgtt cgctcagtcc agcgaaagta cgtgccaaat
      781 gtaaaacaga attatataaa tctgttaaat agggaaacta ctactctgtc cctcggaaat
      841 ggatgttgca attatcctgc gtcgactcat tttgtagtga tatagaataa gtagtagttg
      901 acagtttatc cacaactctg ctactagtag aacctcttcc actcgataaa ccatatcttt
      961 taattaaatg tactgcattc taatgatagg tgctgaatat ttcatgaaac ttattcattg
     1021 tgcagatatt catttggatt cgcctatgga aacgcacatg acaacagaac aggcttccat
     1081 gcgaaacact gagattatcc actcctttat tcgcatgaca gaatatgcta agaagaatga
     1141 agttcgcgtt gtcattattg caggtgactt gttcgatgga gagagaatca gaacccgtac
     1201 ggtggatgag attcttgatg caatacgcag tactccttca gtggactatc tctaccttgc
     1261 tggaaaccat gatgaatcct ctcacgcctt ctccaaccat gatcttccgc agaacctcaa
     1321 gcaattcaat cgcaagtgga atagttatgt ttatgaggat gtaatgatat ccggaattga
     1381 aatctgcgat gaaaatgcga catctctata cctgaatatc cctcatagag atggctgcgt
     1441 caacattata tctctccatg gtcaggttgg tacaacctgt ggtatagacc agatcaatct
     1501 caacctacta aaaaatcagg gtattgatta tcttgcacta gggcatctgc actcttattc
     1561 cgtacactct ctagataaca aaggtatata ctgctattct ggatgtctgg agggaagggg
     1621 ctttgatgaa tgcgataaaa aaggctttgt tcttctcacg gtagaatcag gcagactcca
     1681 acacgaattt gttccttttt gctgccgcca gttgcatcaa gttgatgttg acatcactgg
     1741 tcttacaaag aattcagaga ttggacagat gatgaaatct gcagtgcaag gtatccagcc
     1801 tgatgatatg gttgagttca tcctaactgg ttacagtgat cctactgcta acattgcagt
     1861 atcatacctg caaaatctca taaataacag ttttttcttt acgaaggtca aaaacgagag
     1921 ccgcatggca atcaacccag aagattataa aaacgacatc tctctgaaag gtgaatttat
     1981 tcgtttggtt cttgacagtg atgccagtga tgaggataag gctgccatta ttcgcacagg
     2041 aatccaagcc ttaacagggg aggaaatcgg attatgaagc ttttgagatg ccacattgaa
     2101 aactttggtg ttctttctaa ctttgacttc gatttctcat ctggccttac tgtaatatat
     2161 agagaaaatg gttttggaaa aagcaccttt gccgccttta ttaaggcaat gttctacgga
     2221 atgccacgtg gcggttctcg aagcgttgtc gagaatgagc gcaaaagata tgatccttgg
     2281 cagggcggca aatatggtgg ctttcttgag ttcgaatacc aagagaccag gtatcgtgta
     2341 acccgttatt tcggtaaaac tgcttctaaa gatacttttc agataatgga tatctcaaat
     2401 cggcaaaata ttactccatt ttcagagaga cttggtgaag agttattcct gttggacgca
     2461 gaatcctttt cccggagtac atatatgccg cagctttctg cccacgatct gaa
//
LOCUS       DALUAJ010000005         5913 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_1328,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000005 DALUAJ010000000
VERSION     DALUAJ010000005.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1328"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="O0X37_00075"
     CDS             complement(<1..437)
                     /locus_tag="O0X37_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19e"
                     /protein_id="HJK61356.1"
                     /translation="MTTVFDIPANALIAKVAEELKNESEIKAPEWSEYVKTGVHKQMP
                     PENPDWWYIRSAAVLRRIYVDGPIGVERMRSVYGGMQDRGSKPSHFRKGSGSIARKVM
                     QQLEAAGYIEKVTGGRSVSAKGRKFLDNIAHSLKEEAVKEAPGL"
     gene            complement(676..1026)
                     /locus_tag="O0X37_00080"
     CDS             complement(676..1026)
                     /locus_tag="O0X37_00080"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HJK61357.1"
                     /translation="MPNSSDTNSYIQTLKPTIWVGKNGCTEDLVEETRRQLEARKVIK
                     IKWLQSCDLDEEEILELAQKTSAEVLDSRGRMVVLGAKNRGKLPITTPVKGKTSPKKQ
                     SARDRIKSFYLEKK"
     gene            complement(1205..1507)
                     /locus_tag="O0X37_00085"
     CDS             complement(1205..1507)
                     /locus_tag="O0X37_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HJK61358.1"
                     /translation="MAPHKKNPAANEIALERIEILFTQAHKNRDNPDLANRYVALARE
                     IAMRRRLRMPRKYRHSFCPACHAYFVPGENIRVRIQHGKVISTCKNCGAVIRSPVR"
     gene            complement(1513..2127)
                     /gene="purN"
                     /locus_tag="O0X37_00090"
     CDS             complement(1513..2127)
                     /gene="purN"
                     /locus_tag="O0X37_00090"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HJK61359.1"
                     /translation="MKRIAVLASGRGSNFQAILDALARGEINGKCVALITDNKDAYAI
                     ERAKCAGVPAIVVNFRDYPTKADFETDLLAAMHSVNADLFVCAGYMRIIGTEIIRAFA
                     GKMINIHPALLPAFPGLHGQRQALEYGVKIAGCTVHFVDEGLDSGPIILQKAVPVLDD
                     DDEEALDERILEQEHIAFPEAVALFCADRLVVSGRHVKILPETP"
     gene            complement(2230..3165)
                     /locus_tag="O0X37_00095"
     CDS             complement(2230..3165)
                     /locus_tag="O0X37_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HJK61360.1"
                     /translation="MNVRWLLFPFLVFLGGCCYGPSSPTIKLAYEAGFTANDVVMSQY
                     FYGWLILLVLIGAGFFRGSLHRPVSPTPWTIKRLGRMFATGASIALVSVCYCFSLQSV
                     PASVSVILLFQFTWIGVIIQALVERKLPSRRTIAAVVILIIGTILAAGLVGSNVLLTA
                     DGVIFGMLSALFYALYMFLLGHTEPEMHPLTRSFVIMSCSLALLLCILSPSYIVSGTA
                     FSGIWIYGVILGLFGCALPMFLFSIGTPRISTGAATILSSSELPASIICAVLILSEAV
                     SWVQWIGIALIFFGIAYPYLGAGNVSEQRSVESGE"
     gene            complement(3215..4546)
                     /locus_tag="O0X37_00100"
     CDS             complement(3215..4546)
                     /locus_tag="O0X37_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HJK61361.1"
                     /translation="MEFGWVALVIICVIVYAVVCGEIKKRNFHPEIFAFMGPCLMVRT
                     SRTGIFDVLARFRRTFLVYGTLGVVVVAACSVLMTVMIIVTAYLTMLIQPDPSPIIQP
                     QNLLLIPGVNDFVPSTFAVWFALVFAVVIHEFGHGLLSRVEKIRVKYTGILALVIPIG
                     AFVEPDEEEIEKSPLATKLRMFAAGITNNLVVGAICILALILLLGMVVPGTVPFVQGV
                     YEGYPADLAGMQPGTVILAIDGVPVANTTDISTLLAGTIPGQTIQVLGEYKGVQQMYD
                     ITLTSVPPDITGSSAGNTTSGFMGVLYGDPQAITDTLHAWTHPETLGDVLVSALNFLV
                     LPFSSLAGSSSFGMLVTDTPDPAFLSAPFDGFWEIIHVLYWCAWVNILLGTFNALPIG
                     PLDGGQMLREGVKSFLAKRGKEEYALPLCSVITNLLIVVIIIPIIMPYFFR"
     gene            complement(4694..5335)
                     /gene="rnhB"
                     /locus_tag="O0X37_00105"
     CDS             complement(4694..5335)
                     /gene="rnhB"
                     /locus_tag="O0X37_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HJK61362.1"
                     /translation="MKICGVDEAGKGSVLGPMVTAGVLVLDTAELVGLGLRDSKKLTP
                     HRREELYEEITCRWKTAVVVRTPAEIDARSGSMNEFTASCHAAVIQSLVPDVVYVDAC
                     DVNAVRFGSHVRELSGMTDLQMISEHKADDKFLIVGAASIVAKVTRDRLVTELSNEFG
                     DIGSGYPSDPVTIRFLETYLRENGCPPSCARKTWKTVDEISARMSQKALTDFI"
     gene            5395..>5913
                     /locus_tag="O0X37_00110"
     CDS             5395..>5913
                     /locus_tag="O0X37_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HJK61363.1"
                     /translation="MYLIIVGLGGIGRSLAGIASENGHSVVVIDRDEERCAEILTQYD
                     LLAIAGNATDKAVLEDAGVDRADALVATTSDDALNLMACWLAKRYNVRTLVSIVNQKE
                     HSELFKEVGVRISENPDEIVARSLYVWSENPNTQLLASIAGGSIFEIKVSEGAQGVNK
                     TVREVSDVKDMLY"
BASE COUNT         1680 a         1490 c         1360 g         1383 t
ORIGIN      
        1 agacccggag cttccttgac agcctcttcc tttaaggagt gggcaatgtt gtcgaggaac
       61 ttgcggcctt ttgcggagac actacggccg ccggtaactt tctcaatgta gccggctgct
      121 tcgagctgct gcataacttt tcttgcaatg ctgccgcttc cctttctgaa gtgggacggc
      181 ttagagccgc ggtcctgcat accaccatag accgagcgca tgcgctcgac tccaatcggg
      241 ccgtcaacat agatacgacg cagcactgct gcggaacgga tgtaccacca gtctggattc
      301 tctggcggca tctgcttgtg tactcccgtt tttacgtact cagaccactc gggtgccttg
      361 atctcggact cgttcttgag ttcctcggca acctttgcga tgagtgcatt ggctgggatg
      421 tcaaatactg tagtcataga cttttcctca ctgtaagccc gtgcctttcg gcacgatttg
      481 gttgcacgag actttctctg caacacatga cattaacagt ctgtatgaat tggttcccag
      541 acaataaata agttcgtgag aaaaaattgc aattattctt caggagtacc gaggggttag
      601 gatcaagaga tcctcaacat agtctggata agatattgag tgaaattctg aaaaacagaa
      661 ttagtcttgg aagaattatt ttttctcaag atagaaggat tttattctgt cgcgtgccga
      721 ctgttttttg ggagacgttt ttcctttgac cggagtggtt atgggaagtt ttccccggtt
      781 cttcgcacca agcactacca ttctaccgcg cgagtccagt acttctgctg atgttttctg
      841 ggcaagctcg agaatctctt cttcatcaag atcacaactc tgcagccatt taatcttgat
      901 gacttttctt gcctcaagct gacggcgcgt ttcttccacc agatcctcgg tgcagccgtt
      961 tttaccaacc cagattgtgg gttttagtgt ttgaatatag gagtttgtat cggatgagtt
     1021 gggcacgtgt ggtatccctc gtgccgtata tattgatcag ttctggggat aagagttccc
     1081 ctgtcaaaat gccgtaacat tgttttgaaa caaatcaatc gatgtctccc ggacacaaat
     1141 aaaaaaggat caatcagaaa aaactcttgt aaccacctga aaaacacagc gagacagaag
     1201 atactcatct cacgggagag cgtattacgg caccacagtt cttgcaggtg gagatgactt
     1261 tcccgtgctg gatgcggaca cgaatatttt cccccggtac aaaatatgca tgacaggcag
     1321 gacaaaacga atggcggtac tttcttggca tccgaagacg cctccgcatt gcgatctcgc
     1381 gagcgagtgc aacataccgg ttagcaagat cgggattgtc acggttcttg tgtgcctgag
     1441 taaaaagaat ttcaattctt tcaagcgcaa tctcgtttgc agcaggattt tttttgtgcg
     1501 gtgccatgta actcagggtg tctccggcaa aattttcaca tgacgaccgg acacgacaag
     1561 cctatcagca cagaacaggg caacagcttc gggaaatgca atgtgctcct gctcaaggat
     1621 ccgctcatca agtgcttctt catcatcatc atctaggaca gggactgctt tctgcaggat
     1681 gatcggtccg gagtctagtc cctcatccac aaaatgtaca gtacaccctg caattttgac
     1741 accatactcc aaagcctgac gctgaccatg aagacccgga aacgcaggca gcaaggccgg
     1801 gtggatgttg atcattttcc cggcaaatgc ccggataatt tctgtaccaa ttatgcgcat
     1861 gtacccggca cagacaaaaa gatcagcatt taccgaatgc atggctgcaa gaagatcagt
     1921 ctcaaaatca gctttggtcg ggtagtcacg gaaattcacc actatcgcag gaactccggc
     1981 acattttgcc cgttcaattg cataggcatc cttattgtct gtaatgagag caacacactt
     2041 tccattgatc tccccgcgtg caagggcatc cagtatcgcc tgaaagtttg aaccgcgccc
     2101 cgaagcaagt accgcaatcc gcttcatgtt acaggtatat tggacgcaaa tgggttatta
     2161 cattttggca aaggagagtg tacacaaagg gcagatcctc atcatgaatc ccacaaaaaa
     2221 agtttcagct tactctcctg actccacgga tctttgctcc gagacattcc cagctccaag
     2281 atagggataa gcaattccaa agaagatcag agcaatccca atccactgta cccaggagac
     2341 cgcctcagaa agtataagta cagcacagat gatcgatgcc gggagctctg atgaactaag
     2401 aatggttgca gcacctgttg agatacgggg agttccaatc gaaaacagga acatcggaag
     2461 ggcacaccca aacaggccga ggattacccc ataaatccag atgccggaaa aagcagtgcc
     2521 gctcacaata taggaaggtg agagaatgca aagcaagaga gcaagtgaac aggacatgat
     2581 cacaaaactc cgcgtcagtg gatgcatctc gggttctgta tgaccgagca gaaacatata
     2641 gagcgcatag aacaaagccg ataacatacc aaatatgaca ccatcagcag tcagcagaac
     2701 attactgccc accagacctg cggcaagaat tgttccgatg atcaggatca caactgcggc
     2761 aattgttctc cgtgaaggaa gttttcgttc cacaagagcc tgaatgatca caccgatcca
     2821 cgtaaactga aacaacagaa taaccgatac cgatgcagga acactttgga gagagaaaca
     2881 gtagcagaca cttacgagtg cgattgaagc ccccgttgca aacattcgcc ccagccgttt
     2941 gatcgtccat ggagttggtg agactggtcg gtgtaaagac cctcgaaaaa atccagcacc
     3001 gatcaaaacc aacagaatca gccatccata aaagtactgg ctcatcacca catcgtttgc
     3061 tgtaaatcct gcctcataag ccagttttat tgtgggggat gacggtccgt aacaacaacc
     3121 tccaagaaac accagaaacg gaaacaggag ccagcgaacg ttcataaaat atactcatat
     3181 gagggttttt ccctcataag tactgtcttt tcactcaacg gaaaaagtac ggcatgataa
     3241 tggggatgat gatcacaaca atcagcagat tggtgatcac actgcagaga ggtagagcat
     3301 actcctcttt gccacgtttg gcaaggaaac tcttgacacc ctcgcgcaac atttgacccc
     3361 cgtccaaggg accaattggc agtgcattaa aggtacccaa gagaatgtta acccaggcac
     3421 accagtacag cacgtgaatt atttcccaga atccatcaaa cggagcggag aggaatgcag
     3481 ggtccggtgt atcagttacc agcatgccaa aggaactact accagcaaga gaggagaacg
     3541 gcagaacaag gaagttcaaa gcggacacaa gtacgtctcc aagcgtctcc gggtgtgtcc
     3601 atgcatgcag agtatcagtt atcgcctgag ggtcaccata cagaacgccc atgaatccgg
     3661 aggtagtatt tcctgctgat gaaccagtaa tatccggagg tacagaggtc agtgtgatgt
     3721 catacatctg ctgcacacct ttgtactcac cgagtacctg aattgtctga ccggggatcg
     3781 tccctgcgag aagcgtagag atgtccgtgg tatttgcaac agggacgcca tcaattgcaa
     3841 gaatcaccgt tcctggctgc atgcctgcaa gatctgcagg atatccttca tacaccccct
     3901 gcacaaacgg cactgttccc ggaacaacca tcccaagcaa cagaataagc gcaagaatac
     3961 agatcgcacc aactaccaga ttgtttgtaa tgcccgcagc aaacatacga agttttgttg
     4021 caagaggaga cttttcgatc tcctcttcgt cgggttcaac aaaagcacca atcgggatta
     4081 ccagagccag gattccagta tattttacac gaattttttc cacacgggaa agcaaaccgt
     4141 gaccaaactc atggataacc accgcaaaca ccagtgcaaa ccacactgca aacgtggaag
     4201 ggacaaaatc gttcaccccc ggaataagga gtaaattctg cggctgaata atcggagaag
     4261 gatcaggttg aatcagcatc gtcagatatg cggtaacgat aatcattacc gtcatcagga
     4321 cactgcaggc tgcaacaaca acaacaccaa gcgttccgta caccagaaat gtcctgcgaa
     4381 aacgtgccag tacatcaaag atacccgtcc gagatgtccg aaccatcagg cacggcccca
     4441 taaaagcaaa tatctccggg tggaaattac gctttttaat ttccccgcat accactgcgt
     4501 acacaatcac gcagatgatc accagggcaa cccatccgaa ctccattgtt ctgtattgtt
     4561 ctgcggtgaa aggctaagaa gttgatcgta ggtattcagc tgaatacccg tgaacgaaaa
     4621 cagaagacgg agatgagaag agatcacatc ctgcaatgct catcgaacag tttccaatcc
     4681 aagctgtatc caatcagata aaatccgtga gagctttctg agacatacgg gcactgatct
     4741 catcaaccgt cttccatgtc ttgcgggcac acgacggtgg gcatccgttc tcacgaaggt
     4801 acgtttccag aaaccggatt gttacaggat cagatggata gccgctgcct atatcaccaa
     4861 actcattgga aagttcagta acaagccgat cacgagtaac ttttgccaca atacttgcag
     4921 ctcccacaat cagaaatttg tcgtccgcct tatgttctga gatcatctga agatctgtca
     4981 taccagacaa ttcacgtaca tgcgatccga atcttactgc attgacatca caggcatcca
     5041 catataccac gtccggcaca agagattgta tcactgcagc atgacaggaa gcagtgaact
     5101 cattcattga tcctgaccgg gcatcgatct ctgccggagt tcgtaccacg acagccgttt
     5161 tccaccggca tgtgatctcc tcatagagct cttcgcgacg gtgaggcgtc agcttcttgg
     5221 agtcacgaag accaagaccc acaagttctg cggtgtctaa aaccagcaca ccggcagtta
     5281 ccattggccc gagaaccgaa ccttttcccg cttcgtcaac accgcagatt ttcatcagat
     5341 caatatctat cacagggaat aggtataata ccccacgatc ccaaacgatt cagcatgtat
     5401 ctcatcatcg taggtcttgg cggcatcggt agaagtcttg ccggcatcgc ctccgagaat
     5461 ggtcacagcg tggttgtcat tgatcgtgac gaggaacgtt gtgctgaaat ccttacccag
     5521 tatgatctcc ttgctatcgc cggaaacgcc acggataaag cagtactcga ggacgcaggc
     5581 gtggaccggg cagatgctct tgttgcaaca acaagtgatg atgccctcaa tctgatggct
     5641 tgctggcttg caaagcgcta caatgtgaga acacttgttt ccatcgtcaa ccaaaaggaa
     5701 cattcggagc tgttcaaaga ggttggtgtc cgtatcagtg aaaatcctga cgagattgtt
     5761 gcccgcagtc tgtatgtctg gtcggaaaac cccaataccc aattacttgc atctattgct
     5821 gggggtagta ttttcgagat caaagtcagc gaaggagcac agggggttaa taaaacagta
     5881 cgtgaggtat ctgacgtgaa ggatatgctc tat
//
LOCUS       DALUAJ010000006         2277 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_1743,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000006 DALUAJ010000000
VERSION     DALUAJ010000006.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1743"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            117..1340
                     /locus_tag="O0X37_00115"
     CDS             117..1340
                     /locus_tag="O0X37_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HJK61364.1"
                     /translation="MKFGTLADVETRGKTVLVRVDLNSPIDPSTNTILDDKRFREHVP
                     TIQALEESRVVVLAHQSRPGKKDFTTLEAHADRLERLLGMPVMYVDDIFGRCAKEAVR
                     KAHPGDVLLLENVRFSSEENLTMKPEDAKNTHLVRKLASMADLCVNDAFGTAHRSQPT
                     ITGLPFAMRTVAGLLMEKEVKNLSRVFTSAPKPVTFVLGGTKVDDSIAVAGHVLANGT
                     ADRIAIIGVVANVFYLAKGIDIGAPSANLIKTLGYEGEIAKAKAILDAYPDKVILPEY
                     VAVKENEERREYSVGATPADCPILDMGTESIGVFSKLLSESGTVVFNGPAGMFEERDF
                     ATGTYELLHAASKAEFSVCGGGHTAAVIEQIGLASDFSHLSTGGGACIEFLTGKKLPA
                     LDALEKSWELFGGRI"
     gene            1417..1710
                     /locus_tag="O0X37_00120"
     CDS             1417..1710
                     /locus_tag="O0X37_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61365.1"
                     /translation="MEIKEIKSLDILSVAKFFAVIGLVFGFLFGLLGGFGPAAPLPPH
                     HVFAFAGELFSYILSALFGAVVGVIVGFVGGAVSGFLYNFVANSFGGVKMELE"
     gene            1780..2085
                     /locus_tag="O0X37_00125"
     CDS             1780..2085
                     /locus_tag="O0X37_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HJK61366.1"
                     /translation="METKEVRHVDVWSAAKLCAAINLVITVIIGIIGVVLALLNLSVV
                     GTISGLTGVGTGIVGLIISIVILAVIGMIIGFIVGAIVAVIYNLAAGVFGGLKVELE"
BASE COUNT          514 a          536 c          571 g          656 t
ORIGIN      
        1 cggtctgcca gctctcacat ctttccttcg atgggtttaa tactgtcata atgaatagtt
       61 gtaattacga gtgatcgctg ttctatgaca attgtgagag aggggtgtca gacactatga
      121 aatttggtac gcttgcagat gtagaaaccc gcggaaaaac tgttctcgtc cgtgttgact
      181 taaactcgcc gatcgatccg tcaaccaata ccattcttga cgacaaacgg ttccgtgaac
      241 atgtcccgac cattcaggca ctggaagagt cccgtgtcgt tgttcttgcc caccagagcc
      301 gcccgggcaa aaaggatttc accacactgg aagcccacgc agaccgatta gagcgtctcc
      361 ttggcatgcc ggtgatgtat gtggatgaca tcttcggccg gtgtgccaaa gaggctgtcc
      421 gcaaagcaca tcccggtgat gttctccttc tggaaaacgt ccggttcagc tccgaagaaa
      481 atctcacaat gaagccggag gatgcaaaga atacgcatct ggtgagaaaa cttgcatcaa
      541 tggccgatct ctgcgtcaat gatgcattcg gaaccgccca ccgttcccag ccgaccatta
      601 ccggtctgcc gtttgcaatg cgtaccgtag ccggtcttct gatggaaaag gaggtcaaaa
      661 atctctcccg tgtctttacc tccgcaccaa aaccggtgac ctttgttctt ggcggaacca
      721 aggttgatga ctctattgcg gtagcaggtc atgttctggc gaacggtacc gcagaccgta
      781 ttgcaatcat cggtgttgtg gcaaatgtgt tctatcttgc aaaaggtatt gacatcggtg
      841 ccccgtccgc aaatctgatc aagaccctcg gctacgaagg agagattgcc aaagcaaaag
      901 cgattcttga tgcctacccg gataaggtaa ttctgcctga gtatgtggcg gtaaaagaga
      961 acgaagaacg ccgtgagtat tccgttgggg ccacacctgc ggattgcccc attcttgata
     1021 tgggtactga gtcgatcggc gtcttctcga aactgctctc cgagtcggga acggttgtct
     1081 ttaacggtcc tgcgggaatg tttgaggaga gggattttgc aacgggaacg tatgaactgc
     1141 tgcatgcagc ttcgaaggcc gagttctccg tctgcggagg cggtcacact gcggctgtta
     1201 ttgaacagat cggacttgca tcagacttct ctcacctgtc aactggtggc ggggcatgca
     1261 ttgagttttt gacaggaaag aaacttcccg ctctggatgc gctggaaaag tcctgggaac
     1321 ttttcggcgg aaggatctaa ttttttcttt tttttgggag ttgtttgcag gaattgctga
     1381 gattatatcc aagaaacgtt ataggtattc cttgttatgg aaataaaaga gatcaaatcg
     1441 cttgatatcc tctcagttgc caaattcttt gccgtgatcg gtctggtctt tggttttctg
     1501 ttcggtcttc tgggaggttt tggtcccgca gcccctcttc caccgcatca tgtctttgca
     1561 tttgccggag aactcttctc ctatattctc tccgcactgt tcggagcagt tgtgggtgtg
     1621 attgtgggat tcgtcggtgg tgcagtatcc gggttcctct ataactttgt tgccaacagt
     1681 tttggcggtg tcaagatgga acttgagtaa ctctcttttt tctgcctggg ccggtttccg
     1741 cgatgagtat atgaaggtgc agcgtcaaat atcccgtata tggaaacgaa agaagtcaga
     1801 cacgttgatg tatggtctgc cgcaaagctt tgtgcggcaa tcaatcttgt tatcaccgtc
     1861 atcatcggta tcattggtgt agttcttgcc ctcctcaacc tgtctgtcgt agggacgatc
     1921 tctggtctta ccggagttgg taccggtatt gtcggattaa tcattagtat tgtcatcctc
     1981 gctgtcatag gtatgatcat tgggttcatc gtcggcgcaa tcgtggcagt catctacaat
     2041 ctcgccgcag gtgtcttcgg cggtttaaaa gttgagctgg agtaacgcgg tccaaactcg
     2101 ttttgtgata tgagtcggaa caaattttga tcttttttca atccattctg gaacattccg
     2161 gattctttct gtttatctcc ctactcccca gtgctctttg atgagcattt tttctcaata
     2221 aaaagaatgc ctctcttcta ccactatgta ctgtactcat aagtacagta catagta
//
LOCUS       DALUAJ010000007         1876 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_1810,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000007 DALUAJ010000000
VERSION     DALUAJ010000007.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1810"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /locus_tag="O0X37_00130"
     CDS             <1..1489
                     /locus_tag="O0X37_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HJK61367.1"
                     /translation="YFDKVLVANRGEIAIRVMRACREMGIETVAVYSEADADALHVKY
                     ADEAFLIGAAHPTKSYLNMERILEVAEMAGADAIHPGYGFLAEKAAFSGAVKKAGFTF
                     IGPSEETIAMMGSKIGSKQAMSDAGVPVLPWTRSTEHDEAKAFAESIGYPVIVKASAG
                     GGGIGMTIVRSSEEMDEAIEKSMRTAASAFGDPTVFIEKYLENPRHIEVQVFADSQGD
                     VVHMFERECSIQRRHQKLIEEAPCPIMTPELRERMTQSAITVAKTCNYENAGTVEFLY
                     DNGNYYFMEMNTRLQVEHTVTELITGMDMVRMQIKVAAGEPLPYAQDQIICRGHAIEC
                     RVNAEDPMNNFAADAGKITRYRSPGGPGIRIDSCIHVGYAIPPLYDSMIAKLCARAMT
                     REETIARMRRALMEYVILGVKTTLPLHYAIMNNPEFIAGNTHTHFLEEPEVRKHLPRY
                     MRESETRMHQLADSLGKGKEKTIAVTAAVNAYLAAAAAKQAKGKQ"
     gene            1562..1635
                     /locus_tag="O0X37_00135"
     tRNA            1562..1635
                     /locus_tag="O0X37_00135"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1596..1598,aa:Met,seq:cat)
BASE COUNT          479 a          441 c          530 g          426 t
ORIGIN      
        1 ctacttcgac aaagtcctgg tcgccaaccg cggagagatc gccatccgtg tgatgcgggc
       61 atgccgcgag atgggaattg agaccgttgc ggtctactcg gaagcggatg cagatgctct
      121 gcatgtgaag tatgcggacg aggcattttt aatcggtgca gcacatccaa caaagagtta
      181 tctgaatatg gagaggatcc tggaggttgc ggagatggca ggcgccgatg cgatccaccc
      241 gggatacgga ttccttgcag aaaaggctgc attctccgga gcagtaaaaa aagcaggatt
      301 taccttcatc ggtccatcgg aagaaacgat tgcgatgatg ggctcaaaga tcggatccaa
      361 gcaggcgatg tcggatgccg gtgtcccggt tcttccctgg acacggagta cggagcatga
      421 tgaggcaaaa gcgtttgctg agtccatcgg atatccggtg attgtaaaag cgtccgctgg
      481 aggcggaggt atcggaatga cgatcgttcg cagctctgaa gagatggacg aggcaattga
      541 gaagtcaatg cgtacggctg cctctgcttt cggtgatccg acggttttca tcgagaagta
      601 cctcgaaaac ccccgccaca ttgaggtgca ggtgtttgca gactcgcagg gagatgtggt
      661 gcacatgttt gagcgcgagt gttccatcca gcgccgtcac cagaagctga tcgaggaggc
      721 accgtgcccg atcatgacac cggagcttcg cgaacggatg acccagtcgg caattacagt
      781 tgcaaagacc tgtaattatg agaacgccgg aacggtggag ttcctctacg acaacgggaa
      841 ctactacttc atggagatga acacccgtct gcaggtggaa cacaccgtta cagaactcat
      901 cacgggtatg gacatggtgc ggatgcagat caaggtggca gccggtgagc cgctgcccta
      961 tgcacaggat cagatcatct gtcgcggcca tgcaatcgaa tgccgggtca atgcagagga
     1021 tccaatgaac aactttgcag ccgatgcagg aaagattacc cggtaccggt ctccgggagg
     1081 accgggtatt cgtatcgaca gttgtattca tgtaggatat gcaattccgc cgctgtatga
     1141 ttcgatgatc gcaaagctct gtgcccgtgc catgacccgt gaggaaacaa ttgcacggat
     1201 gcgccgtgcg ttaatggagt atgtgatcct cggcgttaag acaacactcc cgctgcatta
     1261 cgcaatcatg aacaatccgg agttcatcgc aggaaacacg cacacgcatt tcctcgaaga
     1321 gccggaggtc agaaagcatc tcccgcggta catgcgtgag agcgagacgc ggatgcacca
     1381 gcttgcggat tcgctcggaa aaggaaagga gaaaacaatc gcagtgaccg cggctgtgaa
     1441 cgcatatctt gcggcggcag cggcaaaaca ggcgaaaggg aaacagtaat tcccctctct
     1501 ttttccgggg agattcagta acctatatat tttgaaacgt ccttcttata atgcacttct
     1561 tgctgcggtg gcctagctgg ttagggcgcc agactcataa tctggaagtc gggggttcgg
     1621 atccctctcg cagcatttct tttcaaggat ttttgctcag cagtttttcg tacatatcga
     1681 tcatgggaat gtattatcag tatactccgt gaacatgtca gaccagacgt gtcgaaattt
     1741 ttagattttt ttttcgacag atggataggg ggtgtgcctg caggccatcc aggatttttt
     1801 tcattttcta ttagccagta ctactactat gtactgtact tattagtaca gtacaagaag
     1861 aaaagaagaa gaaaga
//
LOCUS       DALUAJ010000008         3647 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_1998,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000008 DALUAJ010000000
VERSION     DALUAJ010000008.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3647)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3647)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3647
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1998"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(55..1041)
                     /locus_tag="O0X37_00140"
     CDS             complement(55..1041)
                     /locus_tag="O0X37_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HJK61368.1"
                     /translation="MLEIYRSADASPIPSRIDTIQSGCWVRMIHPTEDEIAYVCTTLS
                     IPREDIVTLLDDEESPRIEHDDGRTLIIVDTPYVDTDDTDPTVSHPAKATGTTSYTTV
                     PAGFIVMQDIIITVSLRTNPVLQTFIDGKVKNFSTVKKTRFLLQFLYRNARLFVQHLR
                     QIDNLTSTIEHTLYKSTRNEELIEMLRISKSLVYITNALKANEAVLEKLYRFPTPSVR
                     MYEEDEDLLEDTIIENRQALEMAQTYSSTLGHIMDTCASMINNNVNSIMKLFTVVTIL
                     LTIPTMIASFLGMNVIIPGYLSENPFAFHLLIVGSLSISATLLWLLFRRKII"
     gene            complement(1150..1941)
                     /locus_tag="O0X37_00145"
     CDS             complement(1150..1941)
                     /locus_tag="O0X37_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HJK61369.1"
                     /translation="MEFRISLFNLIVAVLFLCLLSAPVSADNASVPGEMPRNFADVPS
                     WIIDSIGIKTPVEGGYTVNEPLSDNLGGFSDINRADAARDESDVPVWILVCAGVSGIA
                     VIVCVTVQFLPILIGRTREASPSPVRDRVFAAITDIPGSSAAVLREKTGIHRATLRYH
                     LSILERENRIISRRAGHIYHYFPNGSSLSYTDTIRMTLCQNTTTQNILSIIEKEPGIS
                     GTLLAARAGITPGTLTWHLRRLEKNDLITISRVGRCMQIFPLDNT"
     gene            complement(1916..2557)
                     /locus_tag="O0X37_00150"
     CDS             complement(1916..2557)
                     /locus_tag="O0X37_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61370.1"
                     /translation="MTSAEYLEVVWPQVYTKISPEDREILTGFTHIWELQELDRTGQG
                     TGFGLTINEAIGRERFALMDAIEELEITEGEYQGITQPELFLDLSEETRKILSSMVRA
                     QRGSGSGIRSGTSISEGETHWFSKNVTSNVTRLAVDLQWENPQDIENNLSITIYSPDR
                     CVFGPFTIAEPLYANPQKMQIYSYISRPGGVAEGEWWYCVKGVKIHGVQNFTI"
     gene            complement(2802..>3647)
                     /locus_tag="O0X37_00155"
     CDS             complement(2802..>3647)
                     /locus_tag="O0X37_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61371.1"
                     /translation="VYLYDISKEAISVVPMADSYSKAIVANKTVFFRYTDGFYSYHPP
                     SGVLKKLNVPIQPILTGFVTDGIHFITMWGDYWPRDLTFMMYDLQTGEQSQIYPGGYP
                     DPSGPMFLSGDYFVYDDSGMLVADPDKRYLHAYNISSGETITLPKEDGYSQYLANVWG
                     NTIVYELFSLLNNAYPDPPQYRMFNLKSMEATSLLLPDGIHPSQVYPPYALQSEWDQE
                     TGVTTVKLVRVDLPPIVHPTTESLVIEQNPAPPTTPAGSSIVLILGLTAFILAGVLTI
                     FWRKK"
BASE COUNT          976 a          750 c          924 g          997 t
ORIGIN      
        1 ctgatactgt agtattcgaa aaaacttagg atcattttcc catcaaacta tttttcagat
       61 gatttttctc cggaacagca accacaagag agttgcggag atgctgagtg atccaacgat
      121 gagaagatga aatgcgaatg gattttcaga gagatatcca gggatgataa cgttcatgcc
      181 gaggaagctt gcaatcatcg tcggaatggt caggagaatg gttacaacgg tgaacagctt
      241 catgatgctg ttgacgttgt tgttgatcat ggaggcacag gtgtccataa tatggccaag
      301 ggtactgctg taggtctggg ccatttcgag tgcctgccgg ttttcgatga tggtgtcctc
      361 gagaagatcc tcgtcctctt catacatccg gacagacggc gttgggaacc gatacaattt
      421 ttctagtacc gcttcgttcg ccttcagggc gtttgtgata tagacgaggg attttgagat
      481 acgaagcatc tcgatgagct cttcgtttct ggtggatttg tagagtgtgt gttcaatagt
      541 actggtcagg ttatcgatct gacgaagatg ctgaacgaag agccgagcgt tgcggtagag
      601 aaactgaaga aggaatctgg tttttttcac cgtggaaaaa ttttttactt ttccatcgat
      661 gaaggtctgg agaaccgggt ttgtccgaag agagacggtg atgatgatgt cctgcatgac
      721 gataaatcca gccggaactg tggtgtagct cgtggtgcca gttgcttttg cgggatgtga
      781 tacagtcggg tcagtgtcgt cagtgtccac gtacggtgtg tccacgatga taagagttcg
      841 accatcatcg tgttcaatac gcggactttc ttcgtcatca agcagggtta cgatgtcttc
      901 ccggggaatg gacagtgttg tacagacgta ggcgatctcg tcttcggttg gatgtatcat
      961 gcgaacccag cagccgcttt gtatggtgtc gattctggac ggaattggtg aagcgtcagc
     1021 ggatcggtag atttccagca ttcggatctc ctcggcagta tttgtgccgg cgttacttga
     1081 tttgtttgta tggagaatat atagagtatc gggaagtact gaaaaccttg aaaaaatagt
     1141 gagatagcat caggtgttat ccaggggaaa gatctgcatg caccgtccta cacgagatat
     1201 cgtgataaga tcgttctttt caagccgtct aaggtgccag gtaagggttc ccggggtaat
     1261 accagcacga gcagcaagta aggtaccgga gatcccgggc tctttttcga tgattgagag
     1321 aatgttttgt gtggttgtat tctggcacag tgtcatgcgg atggtatcgg tatagctaag
     1381 agaggatcca ttcgggaaat aatggtagat gtgtcctgct ctgcgggaga tgattcgatt
     1441 ttccctttcg agaatcgaaa gatgataccg gagtgtcgca cgatggattc cagtcttttc
     1501 tcgaagaact gcagcagagc tgccgggtat atcagtgata gctgcaaaca cgcggtctcg
     1561 aacaggactt ggcgatgctt cacgggttct tccgatcagg ataggcaaga actgaacagt
     1621 aacgcatact ataactgcaa tgccagaaac tcctgcacat acaaggatcc ataccggaac
     1681 gtcagactca tcacgagcag catctgcacg attgatgtcc gagaaacctc caaggttatc
     1741 agagagaggt tcgttgacag tataaccacc ttcaaccgga gttttgatac caatactgtc
     1801 aatgatccac gaggggacat ccgcaaagtt tcgaggcatt tcaccaggta ccgaagcatt
     1861 atcagctgaa accggtgcgg aaaggaggca gaggaaaagt accgcaacaa tcagattaaa
     1921 tagtgaaatt ctgaactcca tggatcttta ctcctttgac acagtaccac cactcccctt
     1981 ccgcaacacc acctggacgt gatatatagg agtaaatttg cattttctgg ggattggcgt
     2041 aaagtggttc ggctatggtg aagggaccaa aaacacacct gtccggggag tatatggtaa
     2101 tactgaggtt gttttcaatg tcctgcggat tctcccactg gagatcaaca gcaagtcgag
     2161 ttacatttga ggtaacattt ttggaaaacc agtgtgtctc tccttcactg atagatgtac
     2221 cactacggat accagatccc gaaccgcgtt gggcacgtac catgctactg agtattttgc
     2281 gtgtttcttc agaaagatct agaaagagtt ccggttgggt gattccctga tattcaccct
     2341 cggttatctc cagttcttca atagcatcca tcagagcgaa gcgttcccta ccaattgctt
     2401 cattgatggt gaggccaaat ccagttcctt gaccagttct atcaagttct tgtaactccc
     2461 aaatatgcgt gaaaccagtg agtatttctc tgtcctcggg ggatattttt gtgtacacct
     2521 gtggccagac aacttcaaga tattcagcag aggtcatctc ggtaccgttg tactgatcca
     2581 tcaggacaag gcttttttgg atccgctcat agttttcttc agcaggagtt gctgcgagaa
     2641 cagggattgc cagcagcaga aacacaatgc ctgccagata aaagattctg aatatgtgtt
     2701 tgattgacag catctccttt gttcgatgtg ctttcattcg tagagagatg tatataatat
     2761 ttctggcagt tacgtcaaaa taaaggtggt agggattctg attatttctt tctccagaaa
     2821 atagtaagaa cgccagccag aataaatgct gtcaggccaa ggatcagaac aatcgatgaa
     2881 ccagccggag ttgttggcgg agcgggattc tgctctatta ctaatgattc ggtagtcggg
     2941 tggacaatcg gagggagatc aactctaaca agttttactg tagttacacc agtttcttga
     3001 tcccattcag actgaagggc ataaggggga tagacctgag atgggtgaat tccatccgga
     3061 aggagcaaag atgtcgcctc catggatttc agattgaaca tacggtactg cggaggatct
     3121 ggatatgcat tgttcaaaag actgaacaat tcatagacaa tggtgttccc ccagacgttt
     3181 gcaagatact gactgtatcc atcttccttt ggaagagtga tcgtttctcc tgatgagatg
     3241 ttgtaagcat ggagatatcg tttatccgga tctgctacaa gcattccaga atcatcatac
     3301 acgaaataat ctccagagag aaacatcggg ccgctaggat cgggatatcc tcctggatat
     3361 atctgactct gttctcctgt ttgaagatca tacatcatga aggtaagatc ccttggccag
     3421 tagtctcccc acatcgtgat aaaatgaatt ccgtccgtaa cgaaacccgt cagtatcggt
     3481 tgtataggta cattcagttt ttttaataca cctgatgggg gatggtacga atagaatcca
     3541 tcagtatatc tgaagaagac ggttttgttg gcaacaatag ctttggaata tgaatcagcc
     3601 atagggacga cagagatcgc ctctttggaa atatcataaa gataaac
//
LOCUS       DALUAJ010000009         2137 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_1999,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000009 DALUAJ010000000
VERSION     DALUAJ010000009.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1999"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="O0X37_00160"
     CDS             <1..536
                     /locus_tag="O0X37_00160"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HJK61372.1"
                     /translation="GNLTTLTCEGCGFSGVVEYPMLYHDLGKKFSIFFAPDKTDREAK
                     LPNVLPAHLLPEMRLRLVHNADDLREKIFIFRDLLDDRIVETVKDSILREMDARHEEK
                     MPDALYYAQDMFGCEGRSLIFVPKKGEEYLEPIKIPFETYEKIKMLMHGIWERPVEGY
                     TVVDKLWVQSAAKKEQA"
     gene            533..1255
                     /locus_tag="O0X37_00165"
     CDS             533..1255
                     /locus_tag="O0X37_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HJK61373.1"
                     /translation="MITDEDVIICPECDHEQKITICPSVNVTTDPEMREKVLSGELFL
                     FTCEKCGYTGFAGYPFVYEDKETQGGFLIYLEPDCPDREVGIDGDMADQVVYRERPMR
                     LVADLNALKEKIFIFEAGLDDRVMELFKMLTLTKLHGDNPDQVPDVLLFTKVDEDEDG
                     KKIFLAAFRDNKYLGIVELPYSLYQTCVVTGEPIWDTPVTECAAIDQEWIADRLKSGG
                     VEQCSCDTEEDEHTCECDHDRN"
     gene            1242..>2137
                     /gene="argH"
                     /locus_tag="O0X37_00170"
     CDS             1242..>2137
                     /gene="argH"
                     /locus_tag="O0X37_00170"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HJK61374.1"
                     /translation="MTGTDQIRKGRLGGDRSNAVAEFLSSATADRRIAYCDLRVDMAH
                     ILMLAKQNLIDPASAKMLLSHLHRYMEKGLPESVFDPAHEDIHAGIEAQLIADCGEDV
                     GGRMHLGRSRNDEVATCLRMQTRLDILEILGGIATLRRALIEQAAQHTHSIMPGFTHF
                     QHAQPTTLAHYLLAYESVFARDAGRLFDAYARLNVSPLGSAAFAGTGFALDREATAAY
                     LGFDRAMTNSMDGVASRDFMLEVLAALSILMTNVSRLCEELILWSSAFIRFVELDDAY
                     CSTSSIMPQKKNPDTAEIMRGKS"
BASE COUNT          545 a          506 c          581 g          505 t
ORIGIN      
        1 atggaaacct cactaccctg acctgtgagg gatgcggatt ctcaggagtt gttgagtatc
       61 cgatgctcta tcatgatctt ggcaaaaaat tctcgatctt ttttgcaccg gacaaaacag
      121 accgtgaagc aaaacttcca aacgttctac cggcacatct gctccccgag atgcgtctgc
      181 ggttggttca caacgcagat gacctccggg agaagatctt catcttccgc gatctgttgg
      241 acgaccggat tgttgagacc gtgaaggatt ccattctgcg cgagatggat gcgcgccacg
      301 aggagaaaat gcccgatgcc ctgtactatg cacaggatat gtttggctgc gaaggacgtt
      361 ccctcatctt tgttccaaaa aaaggagagg agtatcttga accgatcaag attccattcg
      421 agacctatga aaagatcaag atgctgatgc atggtatctg ggagaggcct gtcgaagggt
      481 acaccgttgt tgacaaactc tgggtacaat cagctgcaaa gaaggagcag gcatgatcac
      541 agatgaagat gtgatcatct gccccgagtg tgatcatgaa cagaaaatta ccatctgccc
      601 gtccgtgaat gtaacaacag atccggagat gcgtgaaaag gttctctccg gggaactgtt
      661 cctgttcacc tgtgagaaat gcggttacac cggatttgcc ggatatccgt ttgtctatga
      721 agacaaagaa acgcaaggcg gctttctgat ctaccttgag ccggactgtc ccgatcgtga
      781 ggtgggcatt gacggcgaca tggcagatca ggttgtgtac cgggaacgac cgatgcggct
      841 tgttgcggat ctgaatgcct taaaggaaaa gatcttcatc ttcgaagcgg gccttgacga
      901 tcgggtaatg gaactgttca agatgctgac actcacaaaa ctgcacggtg acaacccaga
      961 ccaggtcccg gatgttcttt tgtttaccaa agtggacgag gatgaggacg gaaagaagat
     1021 ctttcttgca gcattcagag acaacaagta tcttggcatt gttgaactgc cttactcact
     1081 ctatcagacc tgcgtagtaa ccggtgagcc gatctgggat acgccggtca cagagtgtgc
     1141 cgcaatcgat caggaatgga ttgcagaccg gctgaaatca ggcggtgtcg agcaatgcag
     1201 ttgtgataca gaagaggacg aacatacctg cgagtgtgat catgaccgga actgatcaga
     1261 tccgcaaagg acggcttggg ggagatcgaa gtaatgcagt cgcagagttt ctgtcctcag
     1321 caacagctga ccggcgtatt gcctactgtg atcttcgtgt ggacatggca catattctga
     1381 tgctggcaaa gcagaacctg atcgatcctg cctcagcaaa aatgctgctg tcacaccttc
     1441 accggtacat ggaaaaagga cttcctgagt ctgtgtttga tcctgcacat gaggatatcc
     1501 acgcaggaat cgaggcacag ctgatagcag actgcggtga ggatgttggc gggagaatgc
     1561 atctcggccg cagccgcaat gatgaggtgg caacatgcct gcgaatgcag acacgtcttg
     1621 atattctgga aattctcggc gggatcgcca cactgcgtcg tgcattgatt gagcaggcag
     1681 cacagcatac ccacagtatt atgccgggct ttacccactt ccagcacgcc cagccgacaa
     1741 cgcttgccca ctacctgctt gcatacgaat cagtgtttgc cagggatgcg ggacggttgt
     1801 ttgatgcata tgcccgcctt aatgtatcac cgctcggctc tgctgcattt gcaggaaccg
     1861 gttttgctct tgaccgggag gcgaccgcgg catatcttgg atttgacaga gccatgacaa
     1921 actcgatgga cggagttgca tcccgcgact ttatgctgga ggtacttgca gcactttcga
     1981 ttctgatgac aaacgtcagt cgtctgtgtg aagagttgat actttggagc agtgcattca
     2041 ttcggtttgt agagttggat gatgcatact gttcaaccag ttccatcatg ccgcagaaga
     2101 aaaatccgga taccgcagag attatgcggg gtaaatc
//
LOCUS       DALUAJ010000010         3793 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_2348,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000010 DALUAJ010000000
VERSION     DALUAJ010000010.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2348"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            188..655
                     /locus_tag="O0X37_00175"
     CDS             188..655
                     /locus_tag="O0X37_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61375.1"
                     /translation="MKKGFAFLLAIVCVIAFCMVPAAAATVDAADTVDDDLIISTLEI
                     PVYGSKGSATGTIVQGQMSSYSYAIQSGDTEMDIQLSWGSTSNNLALMFTSPTGRIYG
                     PYDDYYESTTPNARFGIHVESAGAVLPYGTWIFDVMGKSVSGTETFTLIINSN"
     gene            702..1517
                     /locus_tag="O0X37_00180"
     CDS             702..1517
                     /locus_tag="O0X37_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HJK61376.1"
                     /translation="MLCLPGLSIADEPKDISYYPGDNLVISPVSGSISTDDTSSPVEK
                     YTNVRTISYKVQHDILSSYGYLTGIPSTFITETLAFFFILLGAGGITLLLTRWKPPED
                     PNSRPRVLLNTIREHPGATQSELQKLTGHSRGSVSVNLHHLSMTSKIRKRVQNGKTRY
                     YLACTPDDEMKGFLRKLTTQENPQKILEAIISNPGISQKELHETTGIPKTTLQWHLSQ
                     LAKYEAIKSTRERNTVHYNVIPDLVLLYNNALEETKQNNTSSEEKEPDNNKES"
     gene            1605..2420
                     /locus_tag="O0X37_00185"
     CDS             1605..2420
                     /locus_tag="O0X37_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HJK61377.1"
                     /translation="MNKQHLLIILLLLISLASTGAAADEGQYYPLDGIHITPQNEPIE
                     NNPDDTTPLVEKTGLFSIKDALHRTFITTYNQIFGFPPVIITELGSLFFLLLGGGVLT
                     GLIIRRTQTRHKNPRTQTIYSLIRKKPGITVAELQDHTGYSRGSINYCLTRLILADRI
                     KKIPLKKTNHYYHVEDKEDRSLEFMKHLIQQERPQKIFRTIMNTPGITQKELAETTDI
                     PTTTLQWHLTQLAKYEVITTSRRKNTISYTVLPEYIWLYHHITRETETTTPND"
     gene            complement(2630..>3793)
                     /gene="feoB"
                     /locus_tag="O0X37_00190"
     CDS             complement(2630..>3793)
                     /gene="feoB"
                     /locus_tag="O0X37_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HJK61378.1"
                     /translation="VTNKWLGFPIFLVVMYLVFQIVFAVGDPFIGWIETIFGDAAVFV
                     GEVLGPSMPDWAVSFICDGVIAGVGSVVVFLPNILLMFLLLAILEDSGYLARVAVIMD
                     QIMHKIGLHGKSFIPMILGFGCSVPAVMGARTLETERERKLTVLMTPYMACSARLPVF
                     VLLVGAFFAPQMQGAVMFSIYLLGIIVALIVGLIMQKTVMKGESSSFVIEMPPYRIPT
                     IKGVILHAVEHGGLFLRKAGLIIFPAVLLIWLLATLPYGVEYGSSESIIGMIGEAIAP
                     IFAPLGWGIPEAAVAVIMGLLAKETVVASLGTLFGVGEEMLGETLVNVFTPLSAYSLM
                     VFVLLYMPCLAAMLTIKQETAWRFATGAAVLMCVVAWVVSCIVYQGGVLLGFG"
BASE COUNT         1215 a         1116 c          742 g          720 t
ORIGIN      
        1 aggttttgtc agacaaaaac agagagattg aaaaaccggg gacagagtgt gcgattttct
       61 ctggaaaatc ggaaacagag agagcagagg cggtgacttc ggatcgcaag agacctgatt
      121 tcggataacc taaacctaat ttcagctcaa atacattata accggcaaac gagaacaaac
      181 gtattgtatg aagaaaggat ttgctttcct gcttgcgatt gtctgtgtga tcgcgttttg
      241 tatggtgcct gctgccgcag caacggttga tgcagcagat actgtcgatg atgacctcat
      301 tatttcaacg ctggagattc cggtgtacgg atcgaaaggc tctgctacag ggacgattgt
      361 acagggacag atgagttcat attcctacgc aattcagtcc ggggacacgg aaatggatat
      421 ccagctctca tggggatcaa cttcaaacaa ccttgctctc atgtttacca gcccgactgg
      481 gagaatctat ggtccgtatg atgactacta tgagtctacc acgcccaatg cacgatttgg
      541 tattcatgtg gagtcagcag gtgcggtgtt gccatacggt acctggatat ttgatgtgat
      601 gggaaaaagt gtaagcggaa cagaaacatt tacgctgatt ataaattcta actaataaaa
      661 tgcacatgat cacaaaactc atctcatgtt cttttttaat tatgttgtgt ctaccaggac
      721 tcagcattgc agatgaacct aaggatataa gctattatcc aggagataat ctagtaatat
      781 cacctgtatc tgggagcatc tcaacagacg atacaagctc tcctgttgag aaatatacga
      841 atgtcagaac aatttcatat aaagttcaac acgacatcct ctcctcgtat ggttatctaa
      901 caggtatccc gtccacattc atcacagaaa ccctcgcctt cttcttcatc cttctcggtg
      961 caggcggtat cacactcctt ctcacccgct ggaaaccccc cgaggatccc aactcgcgtc
     1021 caagggttct gctcaacacc atccgggaac accctggtgc aacccagtca gaactccaga
     1081 aacttaccgg tcactcacgc ggctccgtct ccgtcaatct gcatcacctg agcatgacat
     1141 caaagatccg gaaaagagta caaaacggca agacccgcta ctaccttgca tgcactcctg
     1201 atgatgagat gaaaggattt ctgcgcaaac tcaccaccca ggaaaacccg cagaagatcc
     1261 tggaagcaat catcagcaac cccggaatct cccagaaaga actgcacgaa acaacaggaa
     1321 tccccaagac aacactccag tggcatctct cacagcttgc aaagtatgag gccatcaaaa
     1381 gtacgcggga acgaaacacc gtccactaca acgtcatccc ggaccttgta ctcctctaca
     1441 acaacgctct cgaagaaaca aaacagaaca acacatcatc agaggaaaaa gaaccagaca
     1501 acaacaaaga atcataacca catctctttt ttccagaact caccattcac agaaactctc
     1561 atctacgcaa acgagcaaca caatagtact cgggaaagcc actcatgaac aaacaacatc
     1621 tcctcatcat ccttctcctc ctcatatccc tcgcatccac cggagccgca gcggatgaag
     1681 gccagtacta tcccctggac ggaatacaca tcaccccgca gaatgaaccc atcgaaaaca
     1741 accctgacga caccacaccc ctcgtggaaa aaacaggact gttctcgatc aaagacgcgc
     1801 tgcaccggac cttcatcacc acatacaacc agatcttcgg ctttcctccg gtcatcatca
     1861 ccgaacttgg atcactgttc ttcctcctcc tcggcggggg agtactcacc ggccttatca
     1921 tccgcagaac acaaacccgc cacaaaaacc cgcgtacaca gaccatctac agcctcatcc
     1981 gaaaaaagcc cggcatcacc gtcgcagaac ttcaggatca caccggatac tcccgcggat
     2041 ccatcaacta ctgcctcacc agactcatcc ttgcagacag gatcaaaaaa attccgctca
     2101 aaaaaacaaa ccactactac cacgtcgagg acaaagagga cagatcactt gagttcatga
     2161 aacacctcat tcagcaggaa agaccgcaaa agatcttccg gacaatcatg aacacccccg
     2221 gcatcacgca gaaagaactt gccgaaacaa cagacatccc gacaacaacc cttcagtggc
     2281 accttaccca gcttgcaaaa tacgaagtaa tcacaaccag ccgcagaaaa aataccatat
     2341 cctacacagt acttcccgaa tacatctggc tctatcacca catcaccaga gaaacagaaa
     2401 ccaccacccc caacgactaa cacaaaacaa aatcaaaaaa gaaacttact ttttcaaaga
     2461 agagtggcgg gatgccccct tgcagtggct gcacccccca ctcttccctc cagatctgga
     2521 acaattgccg cagcaggaac ccgcatcaca cagatgattt ttcaccatcc gcacaaacag
     2581 gatgacaatc atcacggcaa ccacaacaaa cactgccgtc cagatcggat cacccgaagc
     2641 caagaagaac ccctccctga tacacaatac aggacaccac ccaggcgaca acacacatca
     2701 gaacagctgc tcctgtggca aacctccagg cagtctcctg cttgatcgta agcattgcgg
     2761 caagacacgg catgtagagg agaacaaaca ccatcagaga gtatgccgac agcggtgtaa
     2821 acacattcac cagcgtctcg ccaagcatct cctcgccaac gccgaagagt gtaccaaggg
     2881 atgcgaccac cgtctccttt gcaagcagac ccatgataac cgcaaccgcg gcttccggaa
     2941 taccccagcc aagcggtgca aagatcggcg caatcgcctc accaatcata ccaatgatcg
     3001 actcagagga accatactca acaccgtacg gaagagttgc aagcagccag atcagcagaa
     3061 ccgccgggaa gatgataagt ccagcctttc tcaggaacag accgccgtgt tcgaccgcat
     3121 gaagaataac acccttaatg gtcgggatac ggtagggcgg catctcaata acaaacgacg
     3181 aagactcacc tttcattacc gtcttctgca taatgagacc aacaatcagc gcaacaatga
     3241 tgccgagcag atagatcgaa aacataaccg caccctgcat ctgcggagca aagaatgcac
     3301 caaccagcag gacaaacacc ggcagccgtg cagaacaggc catatacggc gtcataagaa
     3361 cggtaagctt acgctcacgc tcggtctcaa gagttcttgc acccataacg gccggaacac
     3421 tgcacccgaa tccgagaatc atcggaatga acgactttcc atgcagaccg attttgtgca
     3481 tgatctgatc cataataact gccacacgtg caagataccc tgaatcctcc agaattgcca
     3541 gaagcaggaa catcagcaga atgtttggca ggaacacaac aacggaaccg acaccggcaa
     3601 taactccatc acagataaac gaaactgccc agtcaggcat gcttggacca agaacctctc
     3661 cgacaaacac cgcggcatca ccaaagatcg tttcaatcca cccgataaac ggatcaccca
     3721 cggcaaacac aatctggaac accagataca tcacaacaag gaatatcgga aaaccaagcc
     3781 acttgttggt aac
//
LOCUS       DALUAJ010000011         3457 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_2499,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000011 DALUAJ010000000
VERSION     DALUAJ010000011.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2499"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..2540)
                     /locus_tag="O0X37_00195"
     CDS             complement(<1..2540)
                     /locus_tag="O0X37_00195"
                     /inference="COORDINATES: protein motif:HMM:NF021028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HJK61379.1"
                     /translation="MLKCSMKGDPSAVFPKERSVLLLAAVVLLVCCCLVVPAAAAITL
                     DDVEVNVSGDSCCEISPHPTEKNTYYITVADYVGTGTDLDYYFSVNVTSPLVGEGVVS
                     VEWTKYILWGWDYRGFPLSSNNISNGDSLTSTTVAVHSPTESLSSGEAPYYTAEIIVD
                     DSSSLTLTFYVSTHILIKPMDLTIYANQSSDFPVPRYEYRYEIDRSVFKVDGKDFCFG
                     YENDKYPFTINYYNVTTGYKVICPAEPGTYRAVIEPVDGQDLSEISIKRLSYEYPLLY
                     AEGTLTILPTTKNVMLSPSTSPDTVPAGNKDIFAYIRDCTYSYLNGGYDSKYEERSEH
                     SVHDTVYFLQDDLLDEEAALITDYIRENRPGYDAEMKYFDMVADNIWVELYNSPSVYW
                     PYPEGTDENTEFTLLYFPDTRRSIDITGTIVTTLGEPISLDITKHDHYLYYEYWNYAG
                     LYALIWEDATDPVVIEPVNLTIYANEKFPLPLYSGIPEDAVIKANGQTYPVADPPFKI
                     AYYNTTTNTQISPDTPGIYNATIQPVLDVQLEQITINGNPVLFRNGTLTIRQITDASE
                     TIPVAQQTLGGDRISVSVPEDTTYYLNNNTSLPVTGGNIHLLHDEILAGGEDTLKQIA
                     PEYFDDSSKYEMKYFDLIDTGNGHTWVSSSEDVTITWPYPEGTDENTEFTLLHYLDLH
                     REYFTDEDLNSKTKTVMVSKTGSGVQFSIQRSGFSPFALIWSDQKPVPTPPSGGGSSD
                     GNMDNAFRVLFNDGATTLFVVTDLSYGDKLTKPEDPVKDGYTFAGWHKDAACTQAWDF
                     ADGIAGDMTLYAKWTPVQTVTPTVTPTATASVTPTVTAEPSGLPTTTPT"
     gene            complement(2641..2976)
                     /locus_tag="O0X37_00200"
     CDS             complement(2641..2976)
                     /locus_tag="O0X37_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61380.1"
                     /translation="MAVVIYESAPVVVAGCDQVLFRVVNLPVAEGLFQKQVRLHPKMI
                     VFKIQCIIKFATIVIYDHLMRQFGLKYDNLDQYVLHTAILSPLTLLIFVFSSALLSAQ
                     TFAYLCLSC"
     gene            complement(2978..3217)
                     /locus_tag="O0X37_00205"
     CDS             complement(2978..3217)
                     /locus_tag="O0X37_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61381.1"
                     /translation="MRFPVVRRGTGVCLSVFWVAAAVSAGVRCGIRASEALFCREREN
                     LLGTSADGSASHRSGAGCLRVWSAGLCGRQLFDSR"
BASE COUNT          969 a          851 c          848 g          789 t
ORIGIN      
        1 gtcggggttg tcgtcggaag gccgctcggc tccgcggtaa cagtcggcgt cacggatgcc
       61 gttgcggtgg gcgtgacggt cggcgtgacc gtttgaaccg gcgtccattt ggcgtacagg
      121 gtcatgtcac cggcgatgcc gtcggcaaag tcccatgctt gcgtgcaggc cgcgtctttg
      181 tgccagcccg caaaggtgta gccgtccttt accggatcct ccggtttggt gagtttgtct
      241 ccgtaggaga ggtcggttac gacgaagagc gtggtggctc cgtcgttgaa gagaacacgg
      301 aaggcattgt ccatgttgcc gtcggaggac ccgccgccgg acggcggggt cgggacaggt
      361 ttttggtccg accagatcaa ggcaaaggga ctgaatccgc tccgctggat gctgaactgc
      421 acaccggagc cggtcttgct gaccataacc gtttttgtct tagagttcag atcctcatcc
      481 gtgaagtact cgcggtggag atcaagataa tggagaaggg taaactctgt gttctcatcc
      541 gttccttccg gatacggcca ggtgatggta acatcctctg aggagctgac ccatgtgtga
      601 ccattaccgg tgtcgataag gtcaaagtac ttcatctcgt acttgctgga gtcatcgaag
      661 tactccggtg caatttgttt gagagtatcc tcaccgcctg caagaatttc gtcatgcagc
      721 agatggatat taccaccggt tacaggaagg cttgtgttgt tgttgaggta gtaggtggta
      781 tcctccggaa cagagacaga gatcctgtca cctcccaggg tttgttgtgc taccgggatg
      841 gtctcgctgg catcggtgat ctgccggata gtgagggttc cgttccggaa cagtaccgga
      901 ttaccgttga tggtgatctg ttcaagctgg acatccagga ccggctgaat ggtggcattg
      961 tagatgccgg gagtgtcggg ggagatctgg gtgttggttg tggtgttgta gtatgcgatc
     1021 ttaaacgggg gatcagcaac tgggtaggtc tgaccattcg ccttgattac ggcatcctcc
     1081 gggataccgg aatacagcgg taaagggaat ttctcgtttg catagatggt gagattgacc
     1141 ggttcgatga cgaccggatc cgttgcatcc tcccagatca gagcatagag tccggcataa
     1201 ttccaatatt cataatagag ataatgatcg tgctttgtga tatcaagaga tattggttct
     1261 cctagcgttg tcacgatggt accagtgata tcaatactcc ggcgtgtgtc tgggaagtac
     1321 agtaatgtaa actcagtgtt ttcatcggtt ccttccggat acggccagta gacgctaggg
     1381 ctgttataaa gttctaccca gatgttgtca gcaaccatat caaagtactt catctccgcg
     1441 tcataaccgg gacgattttc ccgaatgtag tctgtaatga gggcagcttc ttcatcaagg
     1501 aggtcgtcct gcaaaaagta cactgtatcg tgaacagagt gttcagacct ttcttcatat
     1561 ttactgtcat aaccaccatt gagatagctg taagtacaat ctcgaatata cgcaaaaata
     1621 tctttatttc cggcaggtac ggtgtcagga gaggtagatg gagacaacat tacattctta
     1681 gtcgtcggga gaatagtcaa ggttccctct gcatagagaa gaggatattc ataacttagg
     1741 cgttttatgg aaatctcaga aagatcctgc ccatccacgg gctcaataac tgccctgtaa
     1801 gtacctggtt ctgctggaca gatcactttg taaccagtag tgacattgta gtagttgatg
     1861 gtaaatgggt acttgtcgtt ctcatatccg aaacaaaaat cctttccatc tactttgaat
     1921 acagatctat ctatctcata acgatattca tacctcggta ctggaaagtc agaactctga
     1981 tttgcataga tggtgagatc cattggttta atgagaatat gtgtgctaac gtagaatgtt
     2041 aacgtcaagg aactgctatc atctacgata atctccgcgg tataataagg agcttcgcct
     2101 gaagacagtg attcagtagg agaatgaact gcaactgtag tagaggttag ggagtcccca
     2161 ttagaaatat tattagaact cagagggaag ccacgataat cccatcccca taatatatat
     2221 ttcgtccact caacagatac caccccttca ccaacaaggg gagaagtaac attcacgcta
     2281 aagtaatagt caagatctgt accagtaccc acataatctg ctacagtaat gtaatacgta
     2341 tttttttccg taggatgtgg cgatatctca caacaactat caccggatac attcacctca
     2401 acatcatcaa gtgttatagc cgccgcagcc ggaacaacca gacagcagca gacgagcagc
     2461 acaacagcgg ccagcagcag cactgacctt tctttgggaa aaaccgcaga cggatctccc
     2521 ttcatagaac atttcagcat agacaactca cctcaaaaaa ggatgccgca gaaactgtcg
     2581 aaacaaaccg ggcaccccgt tgaatcctaa gatgagagga tgccccggag tgactccgtt
     2641 tcagcaggaa aggcagaggt atgcaaaagt ttgtgcagac aacagggccg aactgaaaac
     2701 aaaaatcagc agcgtcaggg gagagagaat agctgtatgc agaacatatt ggtctagatt
     2761 atcgtatttc aacccgaatt gccgcattag atgatcataa ataacgattg tagcaaattt
     2821 aataatacac tgtatcttaa atactatcat cttaggatgt aaacgcacct gtttttgaaa
     2881 caaaccctcc gcaaccggta aattgaccac ccgaaacaga acttgatcac accccgcaac
     2941 aaccaccggc gcagattcat atatcaccac cgccatacta tctactatca aacagctgcc
     3001 gtccgcacaa ccccgcggac cagacccgca gacacccggc accgctccgg tgggacgcag
     3061 aaccatctgc actcgtccca agtaagttct cacgctcacg acagaacaga gcctctgagg
     3121 cacggatgcc acaccggact cccgcggaca cggcagccgc gacccaaaag acggacaaac
     3181 acacacccgt cccgcggcgc acaacaggga accgcacaga acacacctgc ccaacgcaga
     3241 aaaaacactg tgcacacgaa aaaaatcaca acaggaggaa caccacaagc gaacgctctc
     3301 aaaaaaacaa aaaaacctga cagaaaaacg aacaccctgc ccgggaacac cggagcgaag
     3361 agcggagagc gaaaagcgac acacccaaaa caaaaaacat cacgagaaaa aaaatcccgc
     3421 acaccctcat cacccggaag aatgtgctcc gtaaaac
//
LOCUS       DALUAJ010000012         2140 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_2507,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000012 DALUAJ010000000
VERSION     DALUAJ010000012.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2507"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="O0X37_00210"
     CDS             complement(<1..559)
                     /locus_tag="O0X37_00210"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HJK61382.1"
                     /translation="MFLPVSVTENLGRSFQYAKDTLCRTWIEYVIVFVCALIPVVNLL
                     TLPGFVVRVFRNDFALGNWLMLVIDSVKALIITVVYLIIPTVISVVFGAVLGPRNIVT
                     LAVSFVVCLVFVLLLIIALLRFAKGEFGDAFQIGSVFAKISEIGWPNYLIAVLLAYLI
                     LWVLGALCGLLGALGWLIMALLVPFV"
     gene            complement(671..1537)
                     /locus_tag="O0X37_00215"
     CDS             complement(671..1537)
                     /locus_tag="O0X37_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HJK61383.1"
                     /translation="MAIGIVENLRSSYDYTNKSLNSFTDWVILITITMLMLAGVVLVV
                     LGILGMAVTSITTVTPDAVTVTQVPLFDPVGATTVTGTETTVSGSFPLAELFGGAFSL
                     FAMGIGAVLSLVFGILWAGFILRVYRGGELKLGSWGRMFLDGLLATIISLLYCIPYVI
                     ICILLAFGPMTNPTYALIALIVEFIVILITSMVLLMGLIRFAKEQRFGAAFQIKELFS
                     IIGTIGWLRYLANIIVIGIIMLVISVILLLILGIGWVLLLVLVPFLSIWEAKFFANLY
                     ESALPAPAAVEE"
     gene            complement(1618..>2140)
                     /gene="purH"
                     /locus_tag="O0X37_00220"
     CDS             complement(1618..>2140)
                     /gene="purH"
                     /locus_tag="O0X37_00220"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843907.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HJK61384.1"
                     /translation="EAREIMKRKENMRLLILPPEVPADELRTIDGGVLVQRTPAYEEG
                     WKVVSKRAPTEAEAEAMKFAWKIVKYAKSNAIVYANATELVGIGVGQMNRVDSAKIAV
                     MKAAEFGRTMKDTVVASDAFLPFPDTLEVAAAAGATALIQPGGSIRDAEVIAKADELG
                     VAVVFTGTRHFRH"
BASE COUNT          616 a          588 c          482 g          454 t
ORIGIN      
        1 gcacaaacgg taccagaagt gccatgatca gccagccaag ggcacccagc agtccgcaca
       61 atgctccaag cacccacagg ataaggtagg cgagaagaac cgcaatcaga tagttgggcc
      121 agccaatctc gctgatcttt gcaaaaacac tgccaatctg gaacgcatct ccaaactctc
      181 cctttgcaaa ccggagaaga gcaataatca gcaacagtac aaacaccaga cagaccacaa
      241 aggaaacggc aagtgtaaca atattccgcg gtccaagaac ggctccgaat acaacggaga
      301 tgactgtagg aataatcaga tacacaacgg tgatgatcaa tgccttcaca ctgtcgatca
      361 cgagcatcag ccagtttccc agggcaaaat cattccggaa cacacggacc acaaaccccg
      421 gcagtgtcag cagattcaca accggaatca gtgcacacac aaacacaatc acatactcta
      481 tccaagttct gcacagcgta tcctttgcat actgaaagga cctgccaaga ttttcggtta
      541 cagataccgg caaaaacatc cacctcgatt acgtagaaaa cattctgccg tgccagatat
      601 atagttgccg caacaggggg aagtaaacaa aaatcattta caaaaaatgc caaaaacaaa
      661 aatgaggatt ttattcctca acagctgcgg gtgcaggaag tgctgactca tacaggttgg
      721 cgaagaactt tgcctcccag atgctcagga atggtaccag aacaagcagc agcacccatc
      781 caattccgag aatcagcagc agaattacgc tgatgaccag cataataatg ccaatcacga
      841 taatgtttgc taaataccgc agccatccga ttgtgccaat gatgctgaac agctccttta
      901 tctggaatgc tgcaccgaac cgctgttcct ttgcaaaccg gatcagtccc atgagcagta
      961 ccatggaggt gatcagtatc actataaact ctacaatcag ggcgataaga gcgtacgtcg
     1021 ggttggtcat cgggccgaac gccagcagga tacagatgat cacatacggg atacagtaca
     1081 gaagcgagat gatcgttgcg agcagaccgt caaggaacat tctgccccag gatccaagct
     1141 tcagttcacc gccgcggtac acacggagga taaatccggc ccagagaata ccaaagacca
     1201 gggaaagtac tgctccgata cccatggcaa acagtgaaaa tgccccgccg aacagttcag
     1261 ccaacgggaa acttcctgac actgttgttt cagttccggt gacggttgtt gctcctactg
     1321 gatcaaagag cggaacctgt gtcacggtta ctgcatccgg agtaaccgtt gtgattgagg
     1381 ttacagccat accgaggata cccaggacca ccaggaccac acctgccagc atcagcatgg
     1441 tgatggtgat caggattacc caatcggtga aagagtttag tgacttattt gtgtagtcat
     1501 aggaactacg caaattttct acaataccaa ttgccattga tgtatacacc tctttttggg
     1561 aatatcgaaa gttgttccca acggcatata tagtttgtgt aaatcggaaa aagaaaatta
     1621 gtggcggaaa tgccgggttc cggtgaacac cacggccact cccagttcat ctgcctttgc
     1681 aataacttcg gcgtcacgga tcgaaccgcc cggctgaatc agtgcggttg ctccggcagc
     1741 cgcggcaacc tccagcgtgt ccgggaacgg caggaatgca tccgatgcca caaccgtgtc
     1801 cttcattgtt ctgccaaact ctgcggcctt catcaccgca atctttgcag agtccacacg
     1861 gttcatctgg ccgacaccga tacccaccaa ctcggttgcg tttgcgtaca caatcgcatt
     1921 gctctttgca tacttcacaa tcttccaggc aaatttcatg gcttccgctt cagcctcggt
     1981 tggtgcacgt ttggagacaa ccttccaccc ttcttcatat gccggtgttc tctgcaccag
     2041 cacaccgccg tcgatcgtcc gcagctcatc tgccggaacc tcgggcggga ggatcaggag
     2101 acgcatgttc tcctttctct tcataatttc acgtgcttca
//
LOCUS       DALUAJ010000013         2610 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_2704,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000013 DALUAJ010000000
VERSION     DALUAJ010000013.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="O0X37_00225"
     CDS             <1..202
                     /locus_tag="O0X37_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HJK61385.1"
                     /translation="KQLGPVMLPLMLNSLAKADTLGLTIDMRGYRLASEYRRKLVYHA
                     ADAGMILLVILVLAGVAAVTFL"
     gene            complement(266..1159)
                     /locus_tag="O0X37_00230"
     CDS             complement(266..1159)
                     /locus_tag="O0X37_00230"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HJK61386.1"
                     /translation="MQLPKLLPTTVVGSFPCVKGTGLSALFDPYKKAVQFAVAEQIRA
                     GVDIISDGQVRADMVQAFISKLPGISGNTVTSTVGISERPITVADTRYALTKTKYVKG
                     ILTGPCTLAYALKIETPAYRNRDELVLDLATALHSEAKFLAATGAYMIQVDEPILSTG
                     VMDIETAKEALKIIFRDIETPSCIHTCGRLNTISSEWTRLPVDVIDFEYSVSPDNLSD
                     ISRHDLRNKKIGCGCVKSSEPEVESVAEIEKRIRSCVEVFGEENILIDPDCGLRMHTP
                     EVAFAKLKNMCDAAKNVRDEL"
     gene            complement(1213..1902)
                     /locus_tag="O0X37_00235"
     CDS             complement(1213..1902)
                     /locus_tag="O0X37_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HJK61387.1"
                     /translation="MSFLEKISPFERRDLLLAWFCLAVAFTLGISGGLALVTEVGKIS
                     VDMLVLTFVVAVITVGLSFVLHELAHKFMAIRYGYWAEFRKNTQMLLIAVVVAVVTGI
                     VFAAPGATLINTAGREMTKKENGVISLAGPLTNLILAVPFIVCLIAGVLLGGASISAF
                     TVPGFLFYLGMVGFQVNAMLAFFNMLPVGPLDGRKILRWNAVAFVALIAVSLLALYVS
                     LQPYMFVALLL"
     gene            complement(1903..>2610)
                     /locus_tag="O0X37_00240"
     CDS             complement(1903..>2610)
                     /locus_tag="O0X37_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HJK61388.1"
                     /translation="AALTGKTPEYGLHIVKNRLPQIYFRLDDPEAAKKWTCAEYGALG
                     VVAGAIAGNRIPLFMNLRPNRDMLKSLGAAMAASGAVALYHVNEITPETRFPFFKQHI
                     AEDEREVVTIEVADVLNVFKEIEVSAVAIGCPHLSRDEVARVAELLSGKKTVMPFYVF
                     VAEEMRQTCADELAVIAKSGAKVVPDTCMVVSPLMDHAGPVMTNSGKAFSYLPGMCGV
                     TPRMGTLEDCIRVGCGE"
BASE COUNT          616 a          712 c          654 g          628 t
ORIGIN      
        1 gaaacaactc gggccggtga tgcttcctct gatgctgaac tcgctcgcca aagctgacac
       61 gcttggtctt acgatcgata tgcgggggta ccgcctggca tccgagtacc ggagaaaact
      121 ggtgtatcat gccgcagatg cgggaatgat tctgcttgta attcttgtac ttgcaggagt
      181 tgcggcagta accttcctct aatttttatt catccaagtt tctgccgcaa tttttcaaaa
      241 aaagatgatt gatattttct gcggattaca gttcatccct gacattcttt gctgcatcac
      301 acatgttctt cagttttgca aacgctactt ccggcgtgtg catccgcagg ccgcagtcgg
      361 gatcgattag aatgttttcc tctccgaaca cttccacaca ggatcggatc cgtttttcga
      421 tctccgcaac cgactccacc tccggctcgg aacttttcac acagccgcag ccgatctttt
      481 tgttccggag atcatgccgt gagatgtcgg agagattgtc agggcttacc gagtactcga
      541 agtcgattac atccaccggc agccttgtcc actccgagga gatggtgttc agtctgccgc
      601 aggtgtgaat acaggacggt gtttcaatgt ccctgaagat gatcttgagc gcttcctttg
      661 cagtttcaat gtccatgact ccggtcgaaa ggatcggctc atccacctga atcatatatg
      721 cgccggttgc cgcaagaaac ttcgcttccg agtgaagggc tgttgcaaga tccagtacca
      781 gttcgtccct gttccgatat gcgggagtct caatcttcaa cgcatatgcc agtgtgcagg
      841 gtccggtgag aattcctttc acgtactttg tctttgtcag agcataccgg gtgtcggcta
      901 ccgtgatagg tctctccgag atgccgactg tactggtgac tgtattcccg ctgattcccg
      961 gaagttttga gatgaatgcc tgaaccatat ccgccctcac ctgcccgtcg gagatgatgt
     1021 caacccctgc ccggatctgt tcggcgaccg caaactgtac cgcttttttg tacggatcga
     1081 acagtgcgga aagaccagta cctttcacgc atggaaagga tccaacaact gtggtcggaa
     1141 gaagtttcgg aagctgcata tctacagcat tcgggaagaa agataaaaaa agtttgtatt
     1201 gttcctaact tctcagagca gaagggcgac gaacatgtac ggctggagtg agacgtagag
     1261 ggcaagaagc gacaccgcaa tcagggctac gaatgccacc gcattccagc ggaggatttt
     1321 ccttccgtcc agtggtccga ccggcagcat gttgaagaat gcaagcattg catttacctg
     1381 gaatccgacc atacccagat agaacaggaa tcccggaaca gtgaacgcac tgatcgatgc
     1441 gccgccaagc agaactcctg caatcagaca tacaatgaac ggaaccgcaa ggatgaggtt
     1501 tgtcagcggg cctgcaagag agatgacacc gttttctttc tttgtcatct cgcgtcctgc
     1561 ggtattgatc agcgttgctc cgggtgctgc aaacacaatg cccgtaacga ccgcaaccac
     1621 tacggcgatc agaagcatct gcgtgttttt ccggaactct gcccagtaac cataccggat
     1681 tgccatgaac ttgtgggcga gttcgtgcag aacaaacgac agtccaactg taatgaccgc
     1741 aaccacgaag gttaacacca gcatgtcgac tgagatctta ccaacctcgg tgaccagtgc
     1801 gaggcctccc gagataccga gcgtaaacgc aaccgccaga cagaaccaag caagcagaag
     1861 atcgcgtcgt tcgaacgggg atattttttc caggaagctc atttactcac cacacccgac
     1921 acggatacag tcttcaagtg ttcccatccg gggagtaacg ccgcacattc ccggaagata
     1981 cgagaatgct ttgccggagt tggtcataac aggtcctgca tgatccatca gcggagaaac
     2041 caccatgcag gtgtcgggga caacctttgc cccggacttt gcaatcactg cgagctcatc
     2101 ggcacaggtc tgtcgcatct cctctgcaac gaatacgtaa aacggcatca ctgtcttctt
     2161 tccggaaaga agttctgcga cgcgtgccac ttcgtcacgg gaaaggtgcg ggcatccgat
     2221 agcaaccgct gatacttcga tctccttgaa cacattcagt acgtctgcca cctcaattgt
     2281 caccacttca cgttcatcct cggcgatgtg ctgcttgaag aacgggaacc gtgtttccgg
     2341 tgtaatctca ttgacatggt agagggcaac tgcgccggat gcagccattg ctgcgccgag
     2401 gcttttgagc atatcgcgat tcggccgcag attcatgaac agcgggatac ggtttccggc
     2461 aattgcaccg gcaaccactc cgagtgctcc gtactcggcg catgtccact tctttgctgc
     2521 ctccggatca tccagccgga aatatatctg cggcaaccgg ttctttacga tgtggagacc
     2581 gtactccggt gtctttcctg taagtgctgc
//
LOCUS       DALUAJ010000014         3279 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_2742,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000014 DALUAJ010000000
VERSION     DALUAJ010000014.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2742"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..2384
                     /locus_tag="O0X37_00245"
     CDS             <1..2384
                     /locus_tag="O0X37_00245"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HJK61389.1"
                     /translation="KVLDQVQELMDKKSKDSEGAQTELIALMGQIEEKKAERSSILSE
                     RDGIIERSRIRAQESERMKRRQALLVEERAEKQAEIETLEKELSEKQKEKSVFDKQIG
                     EADRSMMSCRRSLEPIREEIGRLTQKLMRLEAQQQVSGESNRAISAVLEMNGVYGTVA
                     RLGKVPSEYTTALNIAAGGRLQNVLVDTDQTASDAIRYLKEERLGRVTFLPLNKIKPA
                     APLPPVAGNGVIDYALNLVEFDEEYRNAFSLVFGQTVVVETLEAGRRLMGRFRMVTLD
                     GELLEKAGAMTGGSIRKDIRGFGAAVDKESAQITAKLSELKNEESDLTSAELRYKAVA
                     DGLRAEKSELDAAITKLELMITECNRRLDAIADEEMSITESLSGMEHDAREVSAKVAE
                     LETRQDELTDSISAMNSRITEIRSVLDESEFTLLTEKLQKARRDADDIKRRLDTKEAD
                     LADVNRERQYFKKRLEERTADKDALAAKNLKLDEEIAVCNQQIADATSAIAALEERQR
                     SFTGELELLRNERAALQTKVDESQARLLSLSSDIERCRVQIAALDEKAAAVSAEIEEI
                     RGSVSDEIECTLSVDEIEDGIAVTERAIRKLGAVNMLAIEQYDEAVAKSEERTSRKDV
                     LSREREALLERIESFAQMKFDAFMAAYSSINTNFQDIFARLTMGSGHLVLDNPEDPFL
                     GGMTFEVRPRDKEVHRLNMMSGGEKSLTTLSFIFAIQRYMPAPFYAFDEVDMSLDGAN
                     VERISQMVRELSSSSQFVIVSLRKPMIEAADRIMGVTIRPDKSTLVTGVKTNV"
     gene            2377..3132
                     /locus_tag="O0X37_00250"
     CDS             2377..3132
                     /locus_tag="O0X37_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HJK61390.1"
                     /translation="MSEEPVEILVQMAERGEIDPWNIDIVEVTDRFLAELERRRELDL
                     RVSGRTLFYSSVLLRMKSEYLDSPQESEDEEEFSDEDPFSGDADPFAEDVVTARALGP
                     IEMLEREIDRRIKRKDARKRPVTLYELITQLKLAEKAERRRQRRRKFVSDDEAFPEPD
                     ADDVVGIAHDEAYDAMAQQIYAIVREHPDARDPGVSLYELVSALHWPLYQVYIPCLFL
                     MLDGLVVLEQDEFFGDLWVVISDGCSTKSEETA"
BASE COUNT          815 a          632 c          983 g          849 t
ORIGIN      
        1 aaaaggtgct tgatcaggtt caggagctga tggataaaaa gagcaaagac tctgaaggtg
       61 ctcagacaga actcattgcc cttatggggc agattgagga aaaaaaggca gaacgttcat
      121 ctattctttc cgagcgtgat ggtattattg agcgcagccg tatccgtgct caggaatccg
      181 aacggatgaa acgccggcag gctctactgg tggaagagcg tgctgaaaag caggctgaaa
      241 ttgagactct ggaaaaggaa ctttccgaga agcaaaaaga gaaatcggtg ttcgataaac
      301 agattggcga agcggatcgc tcgatgatgt cgtgccgccg atctcttgaa cccatccgtg
      361 aagagattgg acgcttaacg cagaaactga tgcggcttga agcacagcag caggtttcag
      421 gagagtcgaa ccgtgccata tctgcagttc ttgaaatgaa cggcgtgtat ggtacagttg
      481 cacgtcttgg aaaggtgccg tctgaatata caactgcatt gaacattgcc gctggcggtc
      541 gtttgcagaa tgttcttgta gatacggatc agaccgcgtc ggatgctatc cgatatctga
      601 aagaggaacg tcttggccgc gtgacctttt taccgctgaa taagattaag cctgcggcac
      661 cgttaccccc tgttgccgga aatggtgtga ttgactatgc actgaatctg gtagagtttg
      721 atgaagagta ccgcaatgcg tttagcctgg tatttggtca gacggtagtg gttgaaacgc
      781 ttgaggcagg caggcgattg atggggagat tccggatggt gacgctggat ggtgaactgc
      841 tggagaaagc cggggcgatg acgggaggtt ctatccggaa agatatccgt gggtttggag
      901 ctgcagttga taaagagtcg gcacagatta ctgcaaaact ctcggaattg aagaatgaag
      961 aaagtgatct gacttcagct gagctgagat ataaagcggt cgcggatggg ctgagggctg
     1021 aaaaaagtga gcttgatgca gcgatcacca aactcgaatt aatgattact gagtgtaacc
     1081 ggcgtcttga tgcgattgca gatgaggaga tgtcgattac cgaatcactt tccgggatgg
     1141 agcatgatgc acgggaagtg tcggcgaaag ttgcagagct tgagacccgt caggatgagt
     1201 tgacggatag catttctgcg atgaactcta gaattacgga gattcggagt gttttggatg
     1261 agtctgagtt tacgctccta acagaaaaac tccaaaaggc tcgtcgtgat gctgatgata
     1321 ttaaacgtcg tcttgataca aaggaagctg atcttgctga tgtaaaccgt gaacggcagt
     1381 acttcaagaa acgcctggaa gagcggactg cggataagga cgcacttgca gcaaagaatc
     1441 tgaaactgga tgaagagatc gctgtgtgca atcagcagat tgccgatgca acttcggcaa
     1501 ttgctgccct tgaagagcgg cagagatcat ttaccggtga acttgagctt ctgcgtaatg
     1561 aacgtgcagc acttcagaca aaggtggatg agtcacaggc acggcttctg tctctttcct
     1621 cggatattga acggtgccgt gtacagattg ccgctttgga tgagaaagca gctgcggttt
     1681 cagcggagat tgaggagatc cgcggtagtg taagcgatga gatcgaatgc actttgtctg
     1741 tggatgagat tgaggatggc attgcggtga cagagcgtgc aatccggaaa ctcggtgcgg
     1801 tgaatatgct ggcaattgag cagtacgatg aggcggttgc gaagtcagaa gagcgaactt
     1861 cccgcaaaga tgtgctttcc cgcgaacgcg aggctctgct tgaacgtatc gagagttttg
     1921 cacagatgaa gtttgatgca ttcatggccg catactcttc cattaatacg aatttccagg
     1981 atatctttgc gcggctaaca atgggctccg gtcatcttgt gctcgataat ccggaagatc
     2041 cgttccttgg aggtatgacg tttgaggtgc ggccgcggga taaggaggta catcggctga
     2101 atatgatgtc tggaggggag aaatcgctga caacgttgtc gtttattttt gcaattcagc
     2161 gatacatgcc tgctccgttc tatgcatttg atgaagtgga tatgtcgctt gatggggcaa
     2221 atgtagagcg tatctctcag atggtgcggg agttgtcttc ttcgagccag tttgtcattg
     2281 tgtctctccg taaaccaatg atagaagcgg cagatcgaat tatgggtgtg acaatccggc
     2341 cagataagag tacgcttgtt acgggcgtga agacgaatgt ctgaggaacc tgtcgagatt
     2401 ctggtgcaga tggcggagcg cggagagatt gatccgtgga acattgatat tgttgaggtg
     2461 acggatcgat tccttgcaga gttggaacgg cggcgtgagc tggatcttcg tgtttcggga
     2521 agaacgttgt tctactcctc ggttcttctc cgaatgaagt ctgagtatct tgattcgccg
     2581 caggagagtg aggacgaaga ggagttttca gatgaggatc ccttttcggg tgatgctgat
     2641 ccatttgctg aggatgtggt aactgcgcgc gctcttggtc cgattgagat gctggagcgc
     2701 gaaattgatc gccgtattaa gcgcaaggat gcgcgcaaac gacctgttac gttgtacgaa
     2761 ctgattactc agcttaagct ggcggaaaaa gcagagcgga ggcgacagag acggcggaag
     2821 tttgttagtg atgatgaggc gtttccggag ccggatgctg atgatgtcgt aggaattgct
     2881 cacgatgaag cgtatgatgc catggcacag cagatttatg cgattgtgcg tgagcatcct
     2941 gatgcgcgtg atccgggtgt ttcgttatat gagcttgtct cggcgctgca ctggccgctg
     3001 taccaggtgt acattccatg cctgtttttg atgctggatg gtctggttgt tctggagcag
     3061 gatgaatttt ttggtgatct ctgggtggta atttccgatg gttgttcaac gaagagtgaa
     3121 gaaacagcat agttttctaa aaattgttgc agttgcacta cttgttctgt gcattgtcgg
     3181 ccttctgatt gttgcagttg gcagtcttgc aatgcagttc ttctaatgtt gctgcaaatg
     3241 ttgtggttat aaatagttct aagttccact ctattccct
//
LOCUS       DALUAJ010000015         2796 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_2906,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000015 DALUAJ010000000
VERSION     DALUAJ010000015.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2906"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="O0X37_00255"
     CDS             <1..718
                     /locus_tag="O0X37_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJK61391.1"
                     /translation="IVQVSFGYTPEKAVLHDISLYAKPGQKVAFVGATGAGKTTITNL
                     INRFYDISAGEILYDGIDVTRMKKSDLRWSLGMVLQDTNLFTGTVMENIRYGKLNATD
                     EEVYAAAKLANADEFIRMLPEGYATMLEGDGGSLSQGQRQLLSIARAAVANPPVMILD
                     EATSSIDTRTEAIVQAGMDSLMKGRTVFVIAHRLSTIHNADVILVLENGKIIERGNHE
                     TLIAQKGKYYQLYTGAFELE"
     gene            782..1141
                     /locus_tag="O0X37_00260"
     CDS             782..1141
                     /locus_tag="O0X37_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJK61392.1"
                     /translation="MDEFCTVNLTVRYLTKKWTLLIILELFKGDNYTRRFSELKERLP
                     DITAKVLSERLQELEAEGIVSKTIDATVVPIRSEYTLTESGIELMEVIRGIKYWALKW
                     KIDNIPCGSQECKLCKL"
     gene            complement(1161..1233)
                     /locus_tag="O0X37_00265"
     tRNA            complement(1161..1233)
                     /locus_tag="O0X37_00265"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1197..1199),aa:His,seq:gtg)
     gene            1433..>2796
                     /gene="infB"
                     /locus_tag="O0X37_00270"
     CDS             1433..>2796
                     /gene="infB"
                     /locus_tag="O0X37_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HJK61393.1"
                     /translation="MAAEHIRTPIVCVLGHVDHGKTSLLDRIRGSRVVAGEAGAITQH
                     IGATMIPIDSIQKMSGELGKLKTTVPGLLFIDTPGHHAFTTLRARGGALADIAILVVD
                     VTEGFKQQTIEALQILRNCKTPFVIAATKIDRIPGWRPMENASFLKSYKNQNERVQNE
                     CENRIYELVGKLSEMGFNSERFDRVSDFQRNLVIVPVSSMTGEGIGDLLMVMIGLAQR
                     FLTEGLKTTVKGPGVGTVLEVKEEKGLGTTLDVILYDGIINVGDEIAIAGTEGAIATK
                     VRALLQPRPMKEILIEDRFQRVKSVTAAAGVKITAPNLEDIIAGSPVRVIRGDRDEML
                     AKIEEEMREINVKLSEIGITVRADTIGALEALSKELEEKNIPIMRAEVGPVSRRDLIE
                     ISVMKDELYKTVLCFNVPLLPDAEAMVRDEEVDVKIFSNRVIYKLIDDYIDWRDELTR
                     AREA"
BASE COUNT          802 a          646 c          732 g          616 t
ORIGIN      
        1 gattgtgcag gtgagttttg gctacacccc ggagaaggca gttctgcacg acatttccct
       61 gtatgcaaaa ccgggacaga aagttgcgtt tgtcggagca accggtgcgg gaaagacaac
      121 tatcacaaac ctgatcaacc ggttttacga tatttctgcg ggagagattc tctatgacgg
      181 catcgatgtg accagaatga aaaaaagcga tctgcggtgg tcacttggaa tggtacttca
      241 ggatacaaac ctcttcacgg gaactgtgat ggaaaacatc cggtacggta aactgaacgc
      301 aaccgatgag gaagtatatg cagcagcaaa gcttgcaaac gcggatgagt ttatccgcat
      361 gcttccggaa ggatatgcaa caatgctgga gggtgatggc ggcagtcttt cacagggaca
      421 acgccagtta ctctccattg cacgtgcagc ggtggcaaac ccgccggtaa tgattctgga
      481 tgaggcgacc tccagtattg acaccagaac agaggctatc gtgcaggcag ggatggactc
      541 actaatgaaa ggccggacag tgttcgtgat tgcccaccgg ctgtcgacga ttcacaatgc
      601 agatgtgatt ctcgttctag aaaacggcaa aataattgag cgcggcaacc atgagaccct
      661 gattgcacag aaaggaaagt actaccagct gtacacgggc gcatttgagc ttgagtgaca
      721 atacatacgt agtatccaaa agatacgaaa gtagatagga gtacactccc catctatttt
      781 tatggatgag ttctgtaccg taaacctgac agtccggtac ctgacaaaaa aatggacact
      841 attaattatt ctcgaacttt tcaaaggaga caactatacc cgcagattct cggaactgaa
      901 agagaggctg ccggatatca cggcaaaagt tctctccgaa cggcttcagg aactagaagc
      961 agaaggaatt gtatcaaaaa cgatcgatgc aaccgttgtt ccaattcgct ccgagtacac
     1021 cctgactgag agcggcattg aactgatgga agttatccgg ggcatcaaat actgggcatt
     1081 aaaatggaag attgacaaca ttccctgtgg cagtcaggaa tgcaaactct gtaaactgta
     1141 ataaaaataa aatacaaaag ggccaaggcc gggactcgaa cccgggtcaa aggatccaca
     1201 gtcctatagg atgtccagct accctacctt agcctgagaa ccagcaaatc tggaccagtt
     1261 acgatcttgc ttcataccat tgtatgaaac actctaatac atagggtgtt cccaaatatt
     1321 aacatatcct ttagatggcg agccaaccgc catttacgtt tattaatgac cactgccaat
     1381 acttacgtag tttttactct gatttttaaa atcagagagg agagaaagag aaatggcagc
     1441 agaacatatc cgtactccca ttgtctgcgt gttggggcac gtggaccatg ggaaaacatc
     1501 gctcctggat cggatccggg gatcgcgggt tgttgccggg gaggctggag cgattaccca
     1561 gcacatcggc gcaacgatga tcccgatcga ttcgattcag aagatgagcg gtgaactggg
     1621 aaaactgaaa acaacagtcc cgggtcttct ctttatcgac acacccggac atcatgcctt
     1681 cacaacactc cgcgcccgtg gcggtgcact tgccgacatt gcaattctgg tggtggatgt
     1741 cactgaagga ttcaaacagc agaccattga agcactccag atacttagaa actgcaagac
     1801 accatttgta attgcggcaa caaagattga ccgtattccc ggctggcgcc caatggagaa
     1861 tgcatcgttt ctgaagtcct ataaaaacca gaacgaacgc gtacaaaacg aatgtgagaa
     1921 ccggatctat gaattagtcg gaaaactttc cgagatggga ttcaactccg agcggtttga
     1981 tcgtgtcagt gacttccagc gcaacctggt gattgttccg gtcagcagta tgaccgggga
     2041 aggtatcggc gatctcctga tggtgatgat cggtcttgcg cagcggttct taaccgaagg
     2101 gctgaagaca acagtaaaag ggcccggtgt gggtaccgta ttggaggtta aagaggagaa
     2161 aggtcttgga acaacacttg acgtgatcct ctatgacggt attatcaacg tcggtgatga
     2221 gattgcaatt gcaggaaccg agggagcaat tgccacaaaa gttcgggcac tgttgcagcc
     2281 tcggccgatg aaagagattc tgatcgaaga tcggttccag cgtgtaaagt cggtcacggc
     2341 agctgcgggt gtgaagatta ccgcgccaaa cctggaagac atcattgcgg gatctcctgt
     2401 ccgtgtaatc cgcggcgacc gtgatgaaat gcttgccaag attgaagagg agatgcgcga
     2461 gatcaacgtg aaactttctg agatcggtat cacggtccgc gcagacacca tcggcgcgct
     2521 tgaagccctc tccaaagaat tggaagagaa gaacatcccg attatgcgtg cagaggtagg
     2581 gccggtcagc cgccgtgatt tgatcgaaat cagtgtgatg aaggacgaac tctacaagac
     2641 ggtgctctgt ttcaatgtcc cgcttctgcc cgatgcggag gcaatggtcc gcgatgagga
     2701 agtggatgta aagatcttct ccaaccgcgt aatctacaaa ctcatcgacg actatatcga
     2761 ctggcgcgac gagcttaccc gcgcacggga agcaaa
//
LOCUS       DALUAJ010000016         2136 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_3199,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000016 DALUAJ010000000
VERSION     DALUAJ010000016.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3199"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="O0X37_00275"
     CDS             complement(<1..628)
                     /locus_tag="O0X37_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HJK61394.1"
                     /translation="MYIGEFIQKLETIAPPVLAEEFDAGRIGLILEGTANIETVACAL
                     DATPHVAEMAAELEVDALVVHHPAFWVPMHGICGRNADVARPLLANNINLYAMHTNFD
                     HADGGINDALADLLGLTDRVKGSLEIIGTMTKPFSAIAEILGCSLRVWGDISEVTRLA
                     VAGGSAFDWELIEEAKDLGAEAYLASELKYNIARESPSPCIEATHYALE"
     gene            696..>2136
                     /locus_tag="O0X37_00280"
     CDS             696..>2136
                     /locus_tag="O0X37_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HJK61395.1"
                     /translation="MYKSIDDINERIHDGSVRVVTAEEMPDIVDELGVSGALREVDVV
                     TTGTFGAMCSSGAFLNFGHPEIPIRMEKMWLNNVEAYAGIAAVDCYLGATQESITRGT
                     EYGGAHVIEDLIAGRSVELHARAKGTDCYPRQSITNEFTLSDLNQAIMVNPRNSYQCY
                     NAATNMGDHAIKTYMGYLLPGHRNVTYSGAGALSPLPNDPTFSVIGSGTPICLGGANG
                     TIIGEGTQHSPGAGFGTLMTTGDMKEMSTDYIRAATMTGYGVTMYVGVGVPIPLLNEE
                     IVKHTAVRDEDLVTSIVDYGTPVRDRPVVRDVTYAELKSGSVEINGEEIRTSSLSSYR
                     KAREVANELKNRIEAGTFTMALATRPINATKKNKPMAEHGVVRRVSEMMVSTFVTITG
                     EESVKEAANRLLKGNTNHLPVIDSENRLIGIVTTFDISKAFACDEEDQKVSEIMTRSV
                     ITALPDEPVDLAARRLQMNNIGALVVIDPT"
BASE COUNT          546 a          500 c          579 g          511 t
ORIGIN      
        1 cttccaatgc gtaatgggtt gcctcaatgc aggggcttgg tgattctcgg gcaatgttgt
       61 acttcagttc ggatgcaaga tatgcttcgg caccaagatc ctttgcctct tcgatcagtt
      121 cccagtcaaa ggctgatccg ccagcaaccg cgagtcgggt tacctcgctt atatcgcccc
      181 agacgcgaag gctacatccg agaatctctg ctattgcgga gaacggtttt gtcatagtgc
      241 cgatgatctc aagtgatccc ttcacgcggt cggtcagacc gagcagatct gccagtgcat
      301 cattgatacc gccgtctgca tgatcaaaat tcgtgtgcat cgcatagagg ttgatgttat
      361 ttgcaagaag tggtctggca acatctgcgt tcctgccaca gattccatgc attggtaccc
      421 agaatgcagg atgatgtacc accagcgcat caacctcgag ttctgctgcc atctctgcaa
      481 catggggtgt tgcatccagg gcacaagcaa ctgtctcaat gtttgctgtt ccttcaagaa
      541 ttagaccaat gcgcccggca tcaaattctt ctgcaaggac aggaggggca atggtctcaa
      601 gtttttggat gaactcaccg atgtacatta gatagatatt ggctttgcgg gattaataag
      661 tcatatatac ctggaacagg gagatattag gctatatgta caaatccatc gatgatatta
      721 acgaacgtat tcacgatgga agtgtgcggg ttgtaacagc cgaagagatg ccggatatcg
      781 ttgatgaact tggcgtgtcc ggagcactcc gggaagtgga tgttgtcaca accggcacct
      841 tcggcgcgat gtgttcatct ggggcgttcc tcaacttcgg acatcctgaa atccccatca
      901 gaatggagaa gatgtggctc aataacgttg aggcatatgc aggtattgca gcggttgact
      961 gttatcttgg ggcaactcag gagtcaataa cccgtggtac cgagtacggc ggggcacacg
     1021 taatcgaaga tctgattgcg ggtcgatcag ttgaactcca tgcacgtgcc aagggtacag
     1081 attgttatcc acgtcagtcc atcaccaatg agttcacatt atccgatctc aatcaggcca
     1141 ttatggtcaa cccgcggaac tcctaccagt gctacaacgc agcaacaaac atgggagacc
     1201 atgcgatcaa gacttatatg ggatatctgc tgcccggtca ccgtaatgtt acctactctg
     1261 gtgctggagc tctatcccct cttcccaacg atcctacatt ctcggtgatt ggttcaggaa
     1321 cacctatctg cctgggaggt gcgaacggaa cgatcatcgg tgagggtact cagcactcgc
     1381 ctggtgctgg gtttgggaca ctgatgacaa ctggtgatat gaaagagatg tcaacggact
     1441 atattcgcgc ggcaacgatg accggatatg gtgtgacaat gtacgtcggt gtgggggttc
     1501 caattccgtt gctaaatgaa gagattgtga aacacactgc ggtacgggat gaggatcttg
     1561 taaccagcat cgttgactat ggtaccccgg tccgtgatcg gccggtggta cgtgatgtga
     1621 catacgcaga actgaagagc ggttcggttg agattaacgg agaggagatc aggacatctt
     1681 ctctgtcaag ctacagaaaa gcacgtgagg ttgcaaacga actgaaaaac agaattgagg
     1741 caggtacgtt tacgatggca cttgcaaccc gcccgatcaa tgcaacgaag aaaaacaaac
     1801 cgatggccga acatggagtt gtccgccgcg tgagcgagat gatggtcagt acgtttgtga
     1861 ccatcaccgg tgaggagagt gttaaggagg cggcgaaccg tctgctgaaa ggaaacacga
     1921 atcacctgcc ggtgatcgac tccgagaacc ggttgatagg tattgtgacg acatttgaca
     1981 tctccaaagc attcgcctgc gatgaggagg atcagaaagt atcagagatt atgacccgca
     2041 gtgtgatcac agcacttccg gatgagccgg tggatctcgc tgcacgcagg ttgcagatga
     2101 ataacatcgg tgcattggtg gtcattgatc cgacaa
//
LOCUS       DALUAJ010000017         4839 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_3664,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000017 DALUAJ010000000
VERSION     DALUAJ010000017.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3664"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="O0X37_00285"
     CDS             <1..430
                     /locus_tag="O0X37_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61396.1"
                     /translation="GHTECTYTPILAATGYASVPLLIGTLITSMVTTTVGGFTTILTA
                     LMTTAVLFWCIPIWVYAVSAVINLPAKAVLHILILPILLIFVVELGSIFVTGSPLGSG
                     VSGGAPVAADPGISMGSGGGMSFSMGTASGPGMGGGPGPR"
     gene            complement(450..1145)
                     /locus_tag="O0X37_00290"
     CDS             complement(450..1145)
                     /locus_tag="O0X37_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61397.1"
                     /translation="MQPANRDRPDHRGTPLRAVIAAVLLLSILLAVPASANTASYYIA
                     ENGTAFSAEVTLVNQSGYLLISPGFIGEGAELQVSNLTLTAEDGTIVNATKASSTQIT
                     FPEGNYTLVYDALLDGRLMYAQYLSPFNTTVFLPAGFHTNNLILGPVSNDGKTYLVSE
                     DMDQLREMGIAVDAAQYETVVVWTDKRAIQMRFYPASYEQYFILCMGLWLIIFCAVGY
                     RYWRLKKNENQYT"
     gene            complement(1088..2287)
                     /gene="thrC"
                     /locus_tag="O0X37_00295"
     CDS             complement(1088..2287)
                     /gene="thrC"
                     /locus_tag="O0X37_00295"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HJK61398.1"
                     /translation="MYRLVCVHCGAEYPPDAIVYNCEKCGHLLAVKYDLDEITVSHEE
                     LQKRPISVWRYKEFLPVRIEPVTLQEGGTPLYHLKRLGEELGLPNLYAKHEGMNPSGS
                     FKDRGMTLGVSMAKQLGKHTVACASTGNTSASMAVYAAKAGMPAVVLLPAGHVALGKV
                     AQALMHGAKVISIRGNFDRALEMVHELCVSHGIYLLNSINPYRLEGQKTIGFEAVDQL
                     GCVPDRIVLPVGNAGNISAVYKGLCEWRDIGYIDTLPMMTAIQAEGAMPVVNAIKGNL
                     PEVVVEQNPETVASAIRIGAPVNAEKALRAIRETHGTAEFVTDAEILAMQRDLARYEG
                     IGVEPASAASVAGIRKLAEQGVLDPNEKIVCVVTGHLLKDPETVIKQCNPPIEIDPTI
                     EALLSVL"
     gene            complement(2321..3079)
                     /locus_tag="O0X37_00300"
     CDS             complement(2321..3079)
                     /locus_tag="O0X37_00300"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HJK61399.1"
                     /translation="MKGSTHILLSVLTGLVILAPVAGLIHWSWSVIILLGIFFGSIAP
                     DVDKGRDSAIFHSSIPGAKGRRFFLTPVIGYFIYIFCYKPLSIIFLGIFGRKILPKQG
                     HRELPHSPIGIICMSVLLTLWLWIFCYALSYIPYLEFLRNNTLIWVFGAAFLIGCFLH
                     LLEDTCDNSGIHYLYPFRFRRVRGTVVGDGSDVRPKMFAVVLLVVAVVVFIGFFTGSI
                     SAEYAKWTAFLLPAALWIVFLKISGVPAKKVAWE"
     gene            3153..4175
                     /locus_tag="O0X37_00305"
     CDS             3153..4175
                     /locus_tag="O0X37_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJK61400.1"
                     /translation="MAAKKSGRTGGQRRKKSQKMQKQIMGILVVIVAAACVLLFGSGA
                     TNSVPELAPLITDEHAFAMHVIDVGQGDAILLSKDNTYALIDAGETMSPSEREAREKL
                     FAYLDSLGIKRLEFLLLTHQDYDHIGSALDVLKTYDVGVVYDNGVTHTSATYENLMQY
                     ILDEDIPYQVVSAGDIIPSPWSEVTLEVLSPQKDNLIMSGSKPDINENSIVMKATYRN
                     VSYLLTGDAEEKAEEAMIDAGMDLKADVLKAGHHGSSSSSTQQFLNEVHPAVIVISVG
                     AGNSYDHPHAEPMERFVAMTKHIYRTDMDGDVVVTTDGEVYSVVTRNPHVYENVIVPN
                     QPAAEA"
     gene            4175..4402
                     /locus_tag="O0X37_00310"
     CDS             4175..4402
                     /locus_tag="O0X37_00310"
                     /inference="COORDINATES: protein motif:HMM:NF022659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HJK61401.1"
                     /translation="MEKLLVTVDAIEGGKAALLLRCPEEERPLAVVPLAMLPEGVAAG
                     DILSLSFHTEPELTEAARARVLALHEKLLKR"
     gene            4399..>4839
                     /locus_tag="O0X37_00315"
     CDS             4399..>4839
                     /locus_tag="O0X37_00315"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJK61402.1"
                     /translation="MTAQFDHAWDDPRLNEGARAYLKTRPENLGDVWERIVRDAVGGD
                     TTELSRVRNKRSVETPASEGVEIVDTDANGVPVRLYLPVSRNRPKKIVLYFHGGGWVV
                     GSIQSCSRFCGELAKAADVIVAAAAYRLAPEFPYPAAGKDCREAY"
BASE COUNT         1213 a         1209 c         1338 g         1079 t
ORIGIN      
        1 aggccatacc gaatgtacat acactccaat ccttgcagcc accggatatg cctctgttcc
       61 gcttctgatc ggaacactca ttaccagtat ggtcacgaca actgtcggcg gattcaccac
      121 aatactcacc gcactgatga caactgccgt tcttttctgg tgtattccca tctgggttta
      181 cgcagtctct gccgtgatca atctcccggc caaagccgta ctgcacatcc tgatccttcc
      241 gattctcttg atcttcgttg ttgaacttgg gagcatcttt gtaaccggct caccacttgg
      301 atcaggggtt tccggaggag caccagtggc agctgatccc ggtatcagca tgggttccgg
      361 aggaggaatg tcattctcca tgggtacagc cagcggaccc ggaatgggcg gcggcccggg
      421 accacggtaa attccttttt tcaaaaaaat taggtgtatt gattttcatt tttcttcaga
      481 cgccagtagc gataccctac tgcacagaag atgatgagcc acaaacccat acagagtatg
      541 aagtactgct cataacttgc gggataaaac cgcatctgaa tcgcacgctt atccgtccag
      601 acaacaaccg tctcatactg cgctgcatca acagcaatac ccatctcgcg cagctgatcc
      661 atgtcctcag aaaccagata cgttttgccg tcattgctga caggaccaag gatcaggtta
      721 ttggtatgaa atcccgcagg aaggaacaca gtggtattga acggcgagag atactgcgca
      781 tacatcaggc gaccgtcaag cagtgcatca tagacaaggg tatagttacc ttccggaaag
      841 gtgatctgcg tggaacttgc cttcgttgca ttgacaatcg taccgtcctc cgcggtgagt
      901 gtaaggtttg agacctgaag ttctgccccc tcaccgataa aacccggaga gatgagcaga
      961 tagccgctct ggtttaccag agtgacctct gcggagaagg ccgtcccatt ctccgcaata
     1021 tagtatgatg ccgtgtttgc ggatgcggga actgccagaa gtatgcttaa cagcagtact
     1081 gctgcaatta cagcacggag aggagtgcct cgatggtcgg gtcgatctcg attggcgggt
     1141 tgcattgctt aatcaccgtc tccggatcct ttaagaggtg gccggtaacc acacaaacga
     1201 tcttctcgtt cggatccagc acaccctgct cggcaagctt acggataccg gcaacggatg
     1261 ctgcggatgc gggttcaaca ccgataccct catagcgggc aagatcgcgc tgcatggcaa
     1321 gaatctctgc atcagttaca aactctgcgg ttccgtgggt ctcacggatt gcacgaaggg
     1381 ctttttccgc gttaaccggt gcaccgatac ggattgcgga tgcaaccgtc tccggattct
     1441 gttcaacaac aacctcgggc aggtttcctt tgattgcgtt gacaacaggc attgcaccct
     1501 ctgcctgaat ggcggtcatc atcggcagtg tgtcgatgta gccgatgtcg cgccactcgc
     1561 acagaccttt gtagactgcg gagatgttgc cggcgttgcc gaccggcaga acaattctgt
     1621 caggaacaca accgagctgg tcaaccgcct caaaaccaat ggtcttctga ccttcaagcc
     1681 ggtacgggtt gatggagttt aagaggtaga ttccatgact gacacagagc tcgtgaacca
     1741 tctcaagggc gcggtcaaag tttccgcgaa tcgagatgac ctttgctccg tgcatcagtg
     1801 cctgagcgac tttgccgagc gcaacatggc ctgccggaag caggacgact gccggcattc
     1861 cagcctttgc ggcatacact gccatggagg cggaggtgtt gccggtggat gcacaggcga
     1921 cggtgtgctt tccaagctgt tttgccattg agacaccaag ggtcataccg cggtccttaa
     1981 aagaaccgga cgggttcata ccctcatgct ttgcatagag gttcggcaga ccgagttctt
     2041 caccgagccg cttgaggtgg tagagcgggg ttcctccttc ctgcagagtc accggctcaa
     2101 ttctgaccgg cagaaattcc ttgtatctcc agaccgagat cgggcgtttc tgcagttctt
     2161 cgtggctgac ggtgatctca tcaagatcat atttgacggc caggaggtgg ccgcatttct
     2221 cacagttgta tacaattgcg tccggaggat actccgcacc gcagtggaca cagacgagac
     2281 gatacatggt ctatgtattt ggtgttgaaa ccttagaatg ttattcccat gcaacttttt
     2341 ttgcaggtac tccggatatc ttcaaaaaga cgatccagag ggcagcaggc aggagaaatg
     2401 ccgtccactt cgcatactct gcagagatac tcccagtgaa aaatccgata aacaccacca
     2461 ctgcaacaac gagaaggaca acggcgaaca tctttggcct gacatcggag ccgtctccga
     2521 caacagttcc gcgaactctg cggaaccgga acgggtagag ataatgaata ccggagttgt
     2581 cgcaggtatc ctccagaaga tgcaggaaac atcctatcag aaatgccgcg ccgaacaccc
     2641 agatgagcgt gttgttccgc agaaactcca ggtacgggat ataggagagc gcgtagcaga
     2701 agatccacag ccacagcgtc agcagcaccg acatacagat gataccaatc ggtgagtgag
     2761 gcagttcacg gtgtccctgt ttcgggagaa tctttctacc gaagatacca aggaagatga
     2821 tggagagggg cttgtaacag aagatgtaga tgaagtagcc gatcactgga gtcaggaaaa
     2881 aacgccggcc ttttgcaccg ggaattgacg agtggaagat cgcagagtca cggcctttgt
     2941 ccacatcagg cgcaatggat ccaaagaaga taccaagcag aatgatcacc gaccatgacc
     3001 agtgaatgag tcctgcaacc ggggccagga taacaagccc ggtcaggacg ctgagcagta
     3061 tatgggttga gcctttcaca aaagatagta tgggatttcc cggaaaaata atcaacggtt
     3121 atttgttttg gaagagtaat agatgagtaa gcatggctgc gaagaaatca ggacgaaccg
     3181 gcggacagcg tcggaaaaag tcacagaaga tgcagaagca gatcatggga atccttgtgg
     3241 tgattgtggc tgcggcatgt gttctgctct ttgggagcgg agcaacaaac tcagttccgg
     3301 aacttgcacc gctgatcacg gatgaacatg catttgcgat gcacgtaatt gatgtaggac
     3361 agggagatgc aattctgctc tccaaggaca acacctacgc gctgatcgat gcgggagaga
     3421 cgatgtcccc gtctgaacgc gaggcacggg agaaactgtt tgcctatctt gattcgctcg
     3481 gtataaagcg gctggagttt ctgctactga cgcatcagga ttatgatcac atcggaagcg
     3541 cccttgatgt gctgaagacc tatgacgttg gtgtggtgta cgacaacggg gtcacccaca
     3601 catcggcaac ctatgaaaac ctgatgcagt atattcttga cgaggacatt ccgtatcagg
     3661 tggtttccgc aggagacatc attccctcac cgtggagcga ggtaacactg gaggtacttt
     3721 caccgcagaa ggataatctg attatgtccg gatcaaagcc ggatattaac gagaactcga
     3781 ttgtgatgaa ggcaacatac cggaacgttt cctatctctt aaccggggat gcggaggaga
     3841 aggctgagga ggcaatgatt gatgcaggta tggatctgaa ggccgacgtt ctgaaagcag
     3901 gacatcacgg aagttcctct tcatcaaccc agcagttcct gaacgaggta catcctgcgg
     3961 tgattgtgat cagcgtcggt gcagggaaca gctacgatca cccgcacgca gagcctatgg
     4021 aacggtttgt tgcgatgaca aaacacatct accggacgga tatggacggt gatgtggtgg
     4081 tcacaaccga cggtgaggtg tactctgtcg tgacacgcaa tccgcatgtg tacgagaatg
     4141 tgattgtgcc aaaccagcca gccgcggagg cataatggag aagctgctgg ttacggttga
     4201 tgcaatcgag ggaggaaagg cagcacttct cctcagatgc ccggaagagg aacgcccgct
     4261 tgcagttgtt cctcttgcga tgctgcccga aggagtggct gcgggagata ttctctccct
     4321 ctcctttcat acagagccgg agctgacaga ggctgcacgg gcgcgggttt tagcactgca
     4381 tgagaaactt ctgaaaagat gacagcacag tttgatcatg cctgggatga tccgcgactg
     4441 aatgagggcg caagagcgta cctaaagacg cggccggaaa atctcgggga tgtgtgggag
     4501 aggattgtac gagatgccgt cggcggtgat acaacagaac tttcccgcgt tcgaaacaag
     4561 aggtctgttg aaacccccgc atcggaaggg gtggagattg tggatacgga tgccaacgga
     4621 gttcccgttc ggttgtatct tcctgtgtcc agaaacagac caaaaaagat cgtactctat
     4681 tttcatggcg gcggctgggt tgtcgggagt atccagagct gttcacggtt ctgcggtgag
     4741 cttgcaaaag cagcggatgt gattgtcgct gcggctgcat accggcttgc accggagttt
     4801 ccgtatcctg ccgcgggaaa agactgccgg gaggcatat
//
LOCUS       DALUAJ010000018         2381 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_3990,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000018 DALUAJ010000000
VERSION     DALUAJ010000018.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3990"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="O0X37_00320"
     CDS             <1..473
                     /locus_tag="O0X37_00320"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HJK61403.1"
                     /translation="DDRCIYAAGDSAGGNLAVVLALQERLSGLILFYPVTTVVPGSYG
                     ESWEEFSHGYALDAKLMEAFAEAYVPEILRDNPLVSPLESADLSRLPQTLCITAECDI
                     LRDQGKRFCDQLRKAGVPVTERCFPGAVHLFVTVTGMGGDFRKGVFECTAFLQK"
     gene            complement(478..1950)
                     /locus_tag="O0X37_00325"
     CDS             complement(478..1950)
                     /locus_tag="O0X37_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HJK61404.1"
                     /translation="MSRNIVTRISAIPPIQRQSMIQLGVTLAITLFGFLSTMLFSHLL
                     GADLMGVYYLFLAYYGVFNMIGDAGFGGAAVKRISEGKDQNEYLTAYAVLRFLLIIVS
                     TLILLAISPYFVDLKEYDLIPWIIAALAAAFFGNTITYGVYGLGHVGIRNLATGVNEF
                     VRILIQIVAVLLGYSLAGLFGGFIIAIIISGIFCIKYFTFRPARFTADHLRSLITYSL
                     WAFLIGSGSLIFSYADTIFIGYFMDNGDVGVYRVALQLTTAGTFLTTAIAGTLTPKFS
                     NWSAKGDLTQIPGILTKSVTYGLLLAVPTAAGGILLSDRLLYFFYGAEFTAGGAACAI
                     LLLLQIVNVFMVFLGTTLSAIDHARQSFYASGSATLLNVALNVLLIPIIGIEGAAVAS
                     LVSITLNAVLLWYYLRQHLAVRCDLRSAGHILLSALLMAAVILVYMQFVLLTNIFLVL
                     VPVAIGAVLYFVVLFRLNREIHDEIADLSKQFGLPWPRWL"
     gene            complement(2014..>2381)
                     /locus_tag="O0X37_00330"
     CDS             complement(2014..>2381)
                     /locus_tag="O0X37_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HJK61405.1"
                     /translation="LLRLPKTLKTQIETAAKLEGITSQEWIRKAVSYRVSLLNVCPSC
                     GTINISTAKYCNECGTSLKESKRTLYMEWMAQNLEEEFGEMGRQLFSDWMKLVDDPEA
                     QIKSIDKDGKWVVVTQEHP"
BASE COUNT          568 a          504 c          765 g          544 t
ORIGIN      
        1 gggatgacag atgtatctat gctgcgggag acagtgcggg aggaaatctc gcagttgtgc
       61 tggcactgca ggaacggctg tccggtctga tcctctttta tccggtgacg acggttgtac
      121 ccggaagcta cggcgagtcg tgggaggagt tttctcacgg atatgcactg gatgcaaaac
      181 tgatggaggc atttgccgag gcgtatgttc cagagatttt gcgggataat ccgcttgtgt
      241 cgccgcttga gtccgcggat ctttcccggc tgccgcagac gctgtgtatc accgcagagt
      301 gtgatattct gcgggatcag gggaagaggt tctgtgacca gctccggaaa gccggagtac
      361 ctgttacaga acgctgtttt cccggagcag tacatctgtt tgtaactgtt accggtatgg
      421 gcggggattt ccggaaaggt gtttttgagt gcaccgcatt tttacagaaa taactcatta
      481 cagccagcgc ggccagggaa gcccgaactg ttttgaaagg tcagcgatct cgtcgtgaat
      541 ttcccggttt agtctgaaca ggacgacaaa gtagaggaca gcaccgatcg caaccggaac
      601 aagaacaagg aagatgttgg tgagcaggac aaactgcatg tagacgagga taaccgccgc
      661 catcaggagg gcactaagga ggatgtggcc tgcggagcgg agatcgcagc ggacggcaag
      721 atgctgcctg aggtaatacc agagaaggac tgcgttgagg gtgatgctga caagagatgc
      781 aaccgcggcc ccttcaatac cgatgattgg gatgagcagc acattgaggg caacgttgag
      841 gagggtggca gatccgcttg catagaagga ctgacgggca tggtcaatgg cgctgagggt
      901 tgtaccaagg aagaccatga acacgtttac gatctggagc agaagcagga tggcacaggc
      961 tgctcctcca gcggtgaact ctgcaccgta gaagaagtaa agcagacgat cggaaagaag
     1021 gataccgcct gcggcagtcg gtacggcaag cagaagtccg taggtgacac tctttgtcag
     1081 gatacccgga atctgagtga gatcaccttt tgcactccag ttgctgaact tgggggtgag
     1141 ggttccggct attgcagtgg tcaggaatgt tcctgcggtg gtgagctgca gtgccacccg
     1201 gtacactccg acatcgccgt tgtccataaa gtagccgata aagatggtgt ctgcatagga
     1261 gaagataaga gagccgctgc cgatgagaaa cgcccagagt gagtaggtga tgaggcttcg
     1321 gagatggtct gcagtaaatc tggcgggacg aaaggtgaag tatttgatac agaatatgcc
     1381 ggagatgata attgcgatga tgaatccgcc aaagagccct gcaagcgagt agccaaggag
     1441 gactgcgact atctggatga ggatgcggac aaactcattg acacctgtgg caaggttccg
     1501 gatgccgacg tgaccaaggc cgtaaactcc gtaggtaatg gtgttcccaa agaatgccgc
     1561 ggcaagcgct gcaatgatcc agggaatgag gtcgtactct ttgagatcaa cgaagtaggg
     1621 gctgatcgca aggaggatca gtgttgagac gatgattaag aggaaacgca gtactgcata
     1681 cgcagtaaga tactcgttct gatcttttcc ttcggagatg cgtttgacgg cggcacctcc
     1741 gaatcccgca tcgcctatca tgttgaagac gccgtagtag gcgaggaaga ggtagtaaac
     1801 gcccatcaga tctgctccga ggaggtggga gaagagcatg gtggagagga atccaaaaag
     1861 ggtgattgcg agggttacgc cgagctggat catgctctga cgctggatgg gcgggattgc
     1921 ggagatgcgg gttacgatgt ttcgggacat ccgtagtatg gagtagtgtt ggtgctgatg
     1981 ggttaagaag gaggcgggga agatcctttg cgatcaggga tgttcctggg ttacaactac
     2041 ccattttccg tctttgtcga tggattttat ctgtgcttca ggatcgtcga cgagtttcat
     2101 ccagtcagag aagagctgcc tgcccatttc gccaaactct tcttcgaggt tctgggccat
     2161 ccattccatg tagagtgtgc gtttgctttc tttgaggctg gttccgcatt cgttgcagta
     2221 ttttgcggtg gatatgttga tggttccgca ggaggggcag acgttgagga ggcttactct
     2281 gtaggatact gctttccgga tccattcctg ggaggttatg ccttcgagtt ttgcggcggt
     2341 ttcgatctgg gttttgaggg ttttcgggag gcgaagaagg a
//
LOCUS       DALUAJ010000019         2044 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_4027,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000019 DALUAJ010000000
VERSION     DALUAJ010000019.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4027"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="O0X37_00335"
     CDS             complement(<1..478)
                     /locus_tag="O0X37_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJK61406.1"
                     /translation="MDDTDTTAGHAGGADLGTAPVISPGVQESETPARAEPAVPKTYA
                     SDSFDPEFFGGIQFNTTADIAIPPSLIDQVIGQEHAVEVIKKAATQRRHVMMIGSPGT
                     GKSMLAKAMAELLPKEEMQDILTYPNAEDNNNPIIRVVPAGRGKEIVAAHRAEARKR"
     gene            574..1419
                     /locus_tag="O0X37_00340"
     CDS             574..1419
                     /locus_tag="O0X37_00340"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HJK61407.1"
                     /translation="MKVIYTEKSQLLASRVARHLGCKVAEVKYNTFPDGEQYVRVMDL
                     DDDMVIVASTVDSQSVLQAILMLDACEGKNTTLVLPYMGYARQDKRFNEGEPISARAL
                     ARALSEDADQIFTVNIHDPSVLSHFKCPAENLTIAQEIGKYILTMNLENPLVLAPDDG
                     AWEFAKGVASVGNWDCDHLDKTRLSGSEVKMAPKHLDAKGRDCIIVDDIIATGGSMAT
                     AAGMLKEQGASSVRAAGVHGVFASGGYVKLMQAGLADIASSDTIERACSKISASEVIA
                     NAVRR"
     gene            1425..1895
                     /locus_tag="O0X37_00345"
     CDS             1425..1895
                     /locus_tag="O0X37_00345"
                     /inference="COORDINATES: protein motif:HMM:NF028455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HJK61408.1"
                     /translation="MRYILDASFFFGEYPFCGEFATTPEVVAELRDVTSKMRFEVMQS
                     RGLVISDPEPAAVTNVRDAAKRSGDLRVLSETDISVIALGLSLSGTVVSDDFAVQNVC
                     RHLKIPVQNMMQKKAKRRIWKKICSGCGAEIPAEDLECPICGSPPVKRGTEKRK"
BASE COUNT          527 a          505 c          543 g          469 t
ORIGIN      
        1 cacgcttgcg tgcctctgca cggtgggctg caacaatctc cttaccgcgt cctgccggaa
       61 ccacacgaat gatcgggttg ttgttgtcct cggcattcgg ataggtgaga atatcctgca
      121 tctcctcttt gggcaggagc tcagccattg ccttggcaag cattgacttt cccgtaccgg
      181 gactgccgat catcatcaca tggcggcgct gggtagccgc tttcttaata acctcaaccg
      241 cgtgctcctg accaatcacc tgatcaatga gcgacggcgg tatcgcaatg tcagctgttg
      301 tattaaactg aatgccgccg aagaactccg gatcgaaact gtccgatgca tatgtcttcg
      361 ggacagcagg ctctgcccgc gcaggagttt ccgactcctg tactccgggg ctgataaccg
      421 gcgcagtccc caaatccgca ccgccagcgt gtcctgccgt agtatctgta tcatccatag
      481 ttatttcctt actcgcctta ctcgagagtg ttaatattgt gcttgatgat ggtttaagta
      541 gtttcagtaa aagatagaag aaggagagta ctgatgaaag ttatctatac cgaaaaaagt
      601 cagctgctgg cctcacgggt ggcccgtcac ctcggatgca aagttgccga ggtcaaatac
      661 aacacattcc ccgatggaga acagtatgtg cgagtgatgg acctcgatga cgacatggtt
      721 atcgttgcaa gcacggtaga ctcccagtca gttcttcagg caatcctgat gctggatgcc
      781 tgcgagggaa agaacaccac actggttctt ccctacatgg gttatgcaag acaggataag
      841 cggttcaacg aaggagaacc aatcagcgca cgggcactcg cacgtgctct ttccgaagat
      901 gcagaccaga tctttaccgt aaacattcac gatccgtctg ttctcagcca cttcaaatgt
      961 cctgccgaga acctcaccat cgcgcaggag attggaaagt acatcctgac gatgaacctc
     1021 gagaaccctc tggtgcttgc accggatgac ggagcatggg agtttgccaa aggtgttgca
     1081 tccgtcggca actgggactg cgaccatctc gacaaaacga gactgtccgg atctgaagtg
     1141 aagatggcac caaaacatct ggatgcaaaa gggcgcgact gcattattgt ggatgacatc
     1201 attgcaacag gcggttccat ggctacagcg gcaggaatgt taaaggagca gggtgccagc
     1261 agtgttcgtg cggcaggcgt tcacggtgta tttgcaagtg gtggctacgt aaagctgatg
     1321 caggcagggc ttgcggatat tgcttcctct gacaccattg aacgtgcatg cagcaaaatc
     1381 tcagcatcgg aagtcattgc aaacgcggtc cgccgataac tttcatgcgc tatatattag
     1441 atgcatcttt ttttttcggg gagtacccgt tttgcggaga gtttgcaaca acgcccgagg
     1501 tggttgcaga gctcagggat gtaacctcaa agatgcggtt tgaggtgatg cagtcaagag
     1561 gactggtaat ctctgatccg gaacccgcag cagtgacaaa tgtccgtgat gccgcaaagc
     1621 ggtccggtga tttacgggta ctttcagaga cggatatctc agtgatagca ctgggactca
     1681 gtctttccgg aacggttgtc tcggacgatt ttgcagtgca gaatgtctgc cgccatctga
     1741 aaattccggt acagaacatg atgcagaaaa aggcaaaacg ccgcatctgg aaaaaaatct
     1801 gcagcggatg tggtgcggag attccggcag aagatttgga atgtccaatt tgcggatctc
     1861 cgccggtaaa gcgcgggacc gaaaagagaa aatgatctga cagaagatgc atcagatccg
     1921 atcacatggg cagttccggc aggcaggatg gaacaaaacc cagaaaaccc aaaagtaata
     1981 attcctgcaa cccgaatatt ttatcacatg tcttcccttg aagagcttat ggcaaaagca
     2041 aaag
//
LOCUS       DALUAJ010000020         1606 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_4239,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000020 DALUAJ010000000
VERSION     DALUAJ010000020.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4239"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            171..1274
                     /gene="cas1c"
                     /locus_tag="O0X37_00350"
     CDS             171..1274
                     /gene="cas1c"
                     /locus_tag="O0X37_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1c"
                     /protein_id="HJK61409.1"
                     /translation="MSPSVIREKAECGIVLTPGDLEINMRKLRNVLYITNPSSYLSRD
                     GESVTVRVDQKEVGRIPIHNLEGIVCFGYMGASPALMELCHNHDVGISFVSPYGKYMA
                     RVGGKVSGNVLLRKKQYAVSDNVEESAKFAKNFILGKLLNCRSVLLRFSRDYPAAVDS
                     AFQAQLDSLSDGILELRDNDLPLNELRGIEGMLSKYYFSCFDDLILSRDPVFSFQGRS
                     RRPPLDPMNALLSFAYTLIAADCASALESVGLDPQVGFLHRLRPGRPSLALDLMEEFR
                     PYLGDRFVVSLVNNRVVKAGDFVMKENGAVLLTDSARKTILHAWQKRKQEEIMHPFLE
                     EKVPLGLLPYAQAMLLARCLRGDLDGYPPFLMR"
     gene            1280..1570
                     /gene="cas2"
                     /locus_tag="O0X37_00355"
     CDS             1280..1570
                     /gene="cas2"
                     /locus_tag="O0X37_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HJK61410.1"
                     /translation="MFVVVTYDVNTETQEGRRRLRKVAKICKNYGVRVQNSVFECVVD
                     SVQFLELKSRIGEVMCAETDSVRYYNLGNEGRRRVEHVGIKPSLNVDEPLIF"
BASE COUNT          446 a          266 c          419 g          475 t
ORIGIN      
        1 ggatgtgtgt attatggtaa aaacaaacgg cgtgtagagg tatcttttga tatggatctg
       61 cgcaaaagag taaaaatggt gattcaggaa atgtactctt tgtttcaaag gggtgaaaca
      121 ccaccagcag tctatcaaaa atattgtgaa aactgttcac tgctgaatct atgtctccct
      181 cagttatccg agaaaaagca gagtgtggca tcgtacttac gccaggagat ctcgagataa
      241 atatgagaaa actaaggaat gtgttgtata ttacgaatcc aagctcatat ttgtcacggg
      301 atggggagag tgtaacggtt cgtgtagatc aaaaagaggt tggccgtatc cctatccata
      361 acctggaagg gattgtgtgt tttggataca tgggtgcaag tccggcactt atggagctgt
      421 gccataatca tgatgttgga atttcttttg tgtctcccta cggaaaatat atggcaaggg
      481 ttggaggaaa ggtttcggga aatgttttgc tgcggaaaaa acagtatgca gtctcagata
      541 atgttgagga atcagcaaaa tttgcaaaga atttcattct gggaaagctg ttgaactgcc
      601 ggagtgtgtt gctgcgtttt tcacgggact accccgctgc agttgattcg gcatttcagg
      661 ctcaacttga ctcactttct gatgggatat tagaacttag agataatgat cttccattaa
      721 atgaacttcg cggtatagag ggaatgttat caaaatatta tttttcatgt tttgatgatc
      781 tgattctttc cagagatcct gttttttctt ttcaggggag gagccggaga ccgcctttag
      841 atccaatgaa tgccctatta tcttttgcat atacattaat tgcggcagat tgtgcatcag
      901 cattggaatc ggtgggtttg gatccacagg tcggtttttt acacagactt cgacctggac
      961 gcccgagttt ggcactggat ctaatggagg agttccgtcc ctatcttgga gacagatttg
     1021 tagtgagtct ggtgaataat cgtgtagtaa aagcagggga ttttgtgatg aaagagaatg
     1081 gagcagttct tttaacagac agtgcacgaa aaacaatact gcatgcctgg cagaagagaa
     1141 aacaggagga gattatgcat ccgttccttg aagagaaggt tcctcttggg cttctgcctt
     1201 atgcccaagc gatgcttttg gcacgttgtt tacgaggaga tttggatggc tatccaccct
     1261 ttctgatgag gtaagcggta tgttcgttgt tgttacgtat gatgtaaata ctgaaaccca
     1321 agagggtaga cgaagattgc gtaaagtggc gaagatatgc aagaattatg gtgtccgtgt
     1381 tcagaactcg gtcttcgaat gtgttgttga ttcagtacag tttctggagt tgaagagtag
     1441 aatcggtgag gttatgtgtg ctgagacgga tagtgtgaga tactataatc tgggaaatga
     1501 aggaagacga cgtgtggaac atgttggcat aaaacccagt ttaaatgtgg atgaaccact
     1561 cattttctaa ctcttcatgc gaagtgagag tgcacatcga tttccc
//
LOCUS       DALUAJ010000021         6739 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_4262,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000021 DALUAJ010000000
VERSION     DALUAJ010000021.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4262"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="O0X37_00360"
     CDS             complement(<1..722)
                     /locus_tag="O0X37_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HJK61411.1"
                     /translation="MERELLREIGIVILVVLVLFSAAVVLFFAEGGEVYGVNHDPTNP
                     VISLEHPEFVIEVIDEEIVPIYDGTEIKTVELWELPPKEIIRQLIISPMAYIPPITSH
                     LITLFVSSLGLAFLVLYRRKDKQLENERPEQILAYLSKNPGHSLQQITDALAINRSTA
                     RHYLRSMEKSKTLSAAGYRGHTHYFAGGSGISETERLMYIILSQEKEKQVIRIIREHP
                     GITKQELAGKLRISPSSCNWYLR"
     gene            1056..1628
                     /locus_tag="O0X37_00365"
     CDS             1056..1628
                     /locus_tag="O0X37_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61412.1"
                     /translation="MSSRETPNLIAFFAVLIAIGVCIVFFCVAWDQNERSTLPDDPTL
                     SRLGIPDKDGLSIVSHGNEITVDNETAVIELIEQFVQENYDYWFAMGSIQVREAAQNG
                     VAIQIHYNPARELICPPAYDRPNATVLIDRIYVTIPDQPDKKNKLYYYVSPVRDGDLD
                     HTILLPIETANELLKLIGEDPIPQNPLFRL"
     gene            complement(1674..2171)
                     /locus_tag="O0X37_00370"
     CDS             complement(1674..2171)
                     /locus_tag="O0X37_00370"
                     /inference="COORDINATES: protein motif:HMM:NF037716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HJK61413.1"
                     /translation="MNFFRPLLLVFVIIILITAGCIGPEQPGDTQYPTTEMTVSPGSE
                     EGSSVLIRISFADQEAIVELYDNAAANDLRSMLPLTLTFRDYNGAEKIAYPPETLDTA
                     NAPAGYDPEAGDVALYAPWGNIAVFYRDSPYASGLVPLGHVVSGLESLAAVSGEFSAT
                     IDVLE"
     gene            complement(2180..2716)
                     /locus_tag="O0X37_00375"
     CDS             complement(2180..2716)
                     /locus_tag="O0X37_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HJK61414.1"
                     /translation="MKHIVVLSGSPRKGGNSDTLCDEFIRGAVDAGNTAEKITISEKK
                     IGYCTACDYCQANGGECIQDDDMTEILEKILACDVLVLATPVYFYSVNAQMKTVIDRV
                     YARYTEISGKEMYYILTAADTSVANMQRTVECFRGFADCLPDAKEKGVIYGIGAWAKG
                     DIKTLPAMQQAYEMGKSV"
     gene            complement(2969..4093)
                     /locus_tag="O0X37_00380"
     CDS             complement(2969..4093)
                     /locus_tag="O0X37_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJK61415.1"
                     /translation="MNEKKLGFGFMRLPLLDENDEASIDIEQTKKMVDVFLERGFTYF
                     DTAWMYCGFRSENAIKEALTDRYPRDRYTIATKLHADFLKSKEDRDAIFYQQLEKTGA
                     QYFDYYLLHDAGRDLYKIYTEMDCFRWLAEKKEQGLIKHIGFYCHDNAEMVDRVFTEH
                     PEMEFVLLQINYIDWENDGIQSRKCYETAVKHGKDVIVMEPVKGGTLARVPEEVERMF
                     KECHPDWSVPSWGIRFAASLEHVKVVLSGMSSLDQMLDNTGYMAEFTPMTEKEIAIVE
                     KAVDLINANITVACTGCSYCTGGCPENIPIPKYFSLYNADLQETKEKIWLPQNQYYAR
                     LAKTFGKASDCIECGQCEQICPQQLPIIQYLKDVAKHFEK"
     gene            complement(4090..4674)
                     /locus_tag="O0X37_00385"
     CDS             complement(4090..4674)
                     /locus_tag="O0X37_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HJK61416.1"
                     /translation="MELHEFLAYMNSGKVITAGTEVHQMMTELAFAAMKITAVLNQGY
                     HEPAEIRHLFSVLTGQTVDESFSLFPPFYTDCGKNIHVGKDVFINSGCHFQDQGGIYI
                     GDGTLIGHSVVLATINHEAEPTIRASMHTKPIVLGKNVWVGSHATILPGVTVGDGAIV
                     AAGAVVTKDVPPGTVVGGVPAKGIKNSVTAGEQK"
     gene            4947..5291
                     /locus_tag="O0X37_00390"
     CDS             4947..5291
                     /locus_tag="O0X37_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJK61417.1"
                     /translation="MTGIDAAVSIISGKWKALILYQLEDGALRYSQILNGMEYGITQR
                     MLTKELREMEESGLISRTVYPETPPKVEYAITEKGRSLMPILDQLCHWGCQHMADQLE
                     YNCAEECENTAE"
     gene            complement(5294..6445)
                     /locus_tag="O0X37_00395"
     CDS             complement(5294..6445)
                     /locus_tag="O0X37_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HJK61418.1"
                     /translation="MRYRHLGKTGLLVSEIGLGAEWLQGRSADEVKAVVDLAADCGIN
                     IVDCWMSEPNVRSNLGAAISGRRADWIIQGHLGSTWQDGQYVRSRDMEKVREAFDDLL
                     VRMQTDHIDLGMIHYVDEAADYRSVMEGEFIAYARSLKKAGVIRHLGMSTHNPAVAKM
                     AAQSGEIEVILFSINPAFDLLPATEDVNTYFAETYDESLGGIAPEREELYRLCEQCGV
                     ALTVMKGFGGGRLFSPKTSPFGVALTPVQCIHYALTRPAVASIMAGCGTSEELKAAGA
                     YETASEEERDYATVLAGAPHHAYFGQCTYCGHCAPCPSGIDVAMVHKLYDLAVMQEGV
                     PESVRDHYRTLSASAEDCIACGSCEERCPFGVHVVERMEKAKDLFSGIR"
BASE COUNT         1622 a         1859 c         1507 g         1751 t
ORIGIN      
        1 cggagatacc agttgcagga ggatgggctt atccgaagtt ttccggcgag ttcctgtttg
       61 gtgatccccg ggtgttcgcg gatgatcctg attacctgtt tctctttttc ctgtgagagg
      121 atgatgtaca tcagccgttc ggtttcggat atcccgcttc ctccggcaaa gtagtgggtg
      181 tgtccgcggt agccggctgc agacagcgtt ttgcttttct ccatacttct gaggtagtga
      241 cgtgcggtgc tgcggtttat ggcaagtgca tctgtaatct gctgaaggga gtgacctgga
      301 tttttgctga ggtaggcgag tatctgttcc ggtctttcgt tttccagttg tttgtcttta
      361 cggcggtaga gtacgagaaa ggcaaggcca agtgaggaga caaaaagggt gatgaggtgg
      421 gaggtgattg gcgggatata tgccatcggt gagataatta gctgccggat aatttccttt
      481 ggcggtagtt cccacagttc gacggtttta atttccgttc cgtcgtagat gggaacaatt
      541 tcctcatcta tcacttcaat tacaaattca gggtgttcga gactaataac tgggttggtt
      601 ggatcatgat taactccata aacctccccg ccttctgcga agaaaagaac aactgctgcg
      661 ctgaacaaaa caagtaccac gagtatgaca atgccgatct cccgcagcag ttctctctcc
      721 atatttatca gttgggtctt cagtgttgta ccggttttgt gatctcccga caactctcaa
      781 atgagatgaa cacctttttc cgggacctca tgaacgaatg ttttctcgtg ttttgttata
      841 gatgtattga ccaaagtgtt ataccctgcc aaccgatgca gatgttattt caccgttttg
      901 ttacgaaaca tgaacgggtg taacagattg gccaatctat tcatatatga taccattgat
      961 agatatggct atgaaccgcg ggctccctca tctcataaat tagcagatat gatctcagtt
     1021 tatttcattg gagtctcacg aggagacgat ctcacatgtc cagccgcgaa acccccaacc
     1081 tcattgcatt cttcgcagtt ctgattgcta tcggtgtctg catcgtcttt ttctgtgtag
     1141 catgggacca gaacgaaaga agtacactcc ctgatgatcc cactctgtca cgtctgggaa
     1201 tcccggacaa ggacggcctc tccattgtat cccacggcaa cgaaatcact gtggacaatg
     1261 aaaccgccgt cattgaactg atagaacagt tcgttcagga aaattatgat tattggtttg
     1321 ccatgggcag catacaagta cgggaagcag cccaaaacgg agttgcaata cagattcatt
     1381 acaacccggc acgcgaacta atctgccctc cggcatacga tcggccaaac gccaccgttc
     1441 tgatcgaccg gatctacgta accattccgg accagccgga taaaaagaat aagctgtact
     1501 actatgtctc cccggttcgc gatggcgatt tggatcacac cattcttctg ccgatagaaa
     1561 cagccaatga acttctgaag ctgatcggag aagaccccat tccgcaaaac cctctgttca
     1621 ggctgtaatc ccgaacagag agacactcat ttttcacaat gatgtccttt tcgttactcc
     1681 agcacatcaa tcgttgcgga aaactccccg cttactgcgg caagactctc cagaccggat
     1741 acaacatgtc cgagcggaac aagcccggag gcatacgggg aatcacgata gaagactgca
     1801 atgtttcccc agggagcata cagtgcaaca tctcccgctt ccggatcgta tcctgccgga
     1861 gcattcgctg tatccagtgt ttctggggga taagcaatct tctctgctcc gttgtagtcc
     1921 cgaaacgtca gcgtcagcgg aagcattgat cgcagatcgt ttgcggctgc attatcatac
     1981 agctccacaa tggcttcctg atcagcgaag cttatccgta tcagcactga tgacccttcc
     2041 tctgaacctg gggacaccgt catctccgtc gtcgggtact gggtatcgcc cggctgttct
     2101 ggaccgatgc atccggcagt gatcaggatg ataatcacga acacaagtag aagaggacgg
     2161 aagaagttca ttgtcttact cagacggatt tacccatctc atacgcctgc tgcattgccg
     2221 gaagtgtttt gatatctccc tttgcccatg caccgatacc atagatgaca cccttctctt
     2281 tcgcatccgg cagacagtct gcaaaacccc ggaaacactc aaccgttctc tgcatattgg
     2341 caaccgacgt atctgccgcg gtcaggatgt aatacatctc tttaccggag atctcagtat
     2401 acctcgcata aacacgatcg atcaccgtct tcatctgggc attcaccgag taaaagtaca
     2461 ccggagttgc cagaacaagc acatcgcatg caagtatctt ctcaagaatc tccgtcatat
     2521 catcatcctg aatacactcg ccgccgttcg cctggcagta atcacacgca gtacaataac
     2581 cgatcttctt ctccgagatt gtgatctttt ctgcggtgtt tcctgcatca accgccccgc
     2641 ggataaactc atcacaaaga gtatccgagt taccgccttt tcttgggctt cctgaaagta
     2701 ctacaatatg tttcataact tctgtttttg tgcaaaagta ccgtacaccc aaacgttgaa
     2761 ctgtgcctgt atggggatac cggcaatctt atccggtgta ctgctgcacc ctccttttct
     2821 ccaggtattt ttgtattctg ccaaaatata atccacagaa acagatctgt tccatgcaca
     2881 cagcaatgat accagctgca tgccgtacct gacctgtaag gaaaaaagat aatttttttg
     2941 ttgtctcttc gattggtttt cccaaaatct acttctcaaa atgttttgca acgtctttga
     3001 ggtactggat aatcggcagc tgctgcggac agatctgctc acactggccg cactcaatac
     3061 agtcgcttgc ttttccgaac gtcttggcaa ggcgggcgta gtactgattc tgcggcagcc
     3121 agatcttctc tttcgtctcc tgaaggtccg cattgtacaa agagaagtac ttggggatgg
     3181 gaatattttc cggacaccct cccgtacagt aggaacagcc agtgcaggca accgtaatgt
     3241 ttgcatttat gagatcaacc gctttttcca cgatcgcgat ctccttctca gtcatcggcg
     3301 tgaactcagc catgtaaccg gtgttgtcaa gcatctgatc cagcgaactc atgccgctca
     3361 ggaccacctt tacatgctcc aaacttgccg caaaacgaat gccccaggaa ggaaccgacc
     3421 agtcgggatg acactctttg aacatcctct caacctcttc cggaaccctt gcaagagtac
     3481 cgcctttcac cggctccatc acgatcacat ccttcccgtg ctttaccgca gtctcataac
     3541 atttacgcga ctggattccg tcattctccc agtcaatata gttgatctgc agcagtacaa
     3601 actccatctc cggatgttcg gtaaagacgc ggtcaaccat ctccgcattg tcgtgacagt
     3661 agaatccgat gtgttttatc agaccctgct ccttcttctc cgcaagccac cggaaacagt
     3721 ccatctccgt gtaaatcttg taaagatcgc gccccgcatc atgcagaaga tagtaatcaa
     3781 agtactgtgc accggtcttt tccagctgct ggtaaaatat tgcatcccgg tcttccttgc
     3841 ttttcagaaa atccgcgtgc agtttcgttg caatcgtgta tctgtcccgc ggataccggt
     3901 cggtaagtgc ctcttttatc gcattttcgc tgcggaaccc gcagtacatc catgccgtat
     3961 caaagtaggt aaatccccgt tcgaggaaaa catcaaccat tttctttgtc tgctcaatgt
     4021 caatactcgc ctcatcgttt tcgtccagaa gaggcagacg cataaatccg aatcctaatt
     4081 tcttctcgtt catttctgtt ctcctgcggt tacggaattt ttgataccct ttgccggaac
     4141 cccgccgaca accgttcccg gcggaacatc cttcgttacc accgcaccgg ccgcaacaat
     4201 cgcaccgtcg ccaacggtca ctcccggcag aatcgttgca tgagatccaa cccagacgtt
     4261 ctttcccaga acgatcggtt ttgtgtgcat gcttgcccgt atcgttggct ctgcctcatg
     4321 gttaatagtg gcaagaacaa ccgaatggcc gataagtgtt ccatccccta tgtagatccc
     4381 gccctgatcc tgaaaatgac atcctgagtt gatgaacaca tcttttccca catggatgtt
     4441 ttttccgcag tccgtataaa acggcgggaa gagtgaaaat gactcatcaa cagtctgtcc
     4501 ggtcagtacg gagaagagat gccgaatctc tgccggctcg tggtagccct ggttgagaac
     4561 agcggtgatc ttcatcgccg caaaggcaag ctctgtcatc atctgatgca cctccgttcc
     4621 ggccgtgatc acctttccgg agttcatata tgccaaaaat tcatgcagtt ccatacatat
     4681 cctctcccca tttcagcggg cggtattcat cccggtccga taccgtttgt ttgcgaatac
     4741 tgccctccat ttcttaggat atcttttgcc ccgttttctc atatagacat tggtaacatg
     4801 gatgttcctt gcacacatgc atgatacctg gaaaactctg tttatctcct ttattcctcc
     4861 ggtttttctc cacaatctat ttccttcgct cctgcaaaca tatactcatg gacggttctg
     4921 cagggcaaaa aaacaaaccg aagtacatga cgggtattga tgcggcggtg agtatcatca
     4981 gcggaaaatg gaaagccctg atcctctacc agctggagga cggagccctg cggtacagcc
     5041 agattctcaa tggtatggag tatggcatca ctcagcgtat gctcaccaaa gagcttcgcg
     5101 agatggaaga gagcgggctt atctcccgga ccgtctatcc ggaaactccg ccaaaggtgg
     5161 agtatgcaat tactgagaaa ggccgatccc tcatgcccat tctcgatcag ttatgccact
     5221 ggggctgtca gcacatggcg gatcagctgg aatacaactg tgcggaagag tgtgaaaata
     5281 cagcagagta actctaccgt atcccgctaa acagatcctt tgctttttcc atccgctcca
     5341 caacatgaac cccgaacgga caccgttcct cacagcttcc gcatgcgata cagtcctcag
     5401 ccgatgcgga aagtgttctg taatgatccc gcacagactc tgggacaccc tcctgcatca
     5461 ccgcaagatc atagagtttg tgcaccattg caacatcgat gccggacggg cacggggcac
     5521 agtggccgca gtaggtacac tgtccaaaat aggcatgatg aggagcacct gccagtacgg
     5581 ttgcgtaatc ccgctcctcc tcagatgccg tctcataggc accagccgct ttcagctcct
     5641 ccgaagtacc gcatcctgcc atgatgcttg caactgcggg cctggttaac gcatagtgga
     5701 tacactgaac cggtgtgagt gccacgccaa agggcgaggt cttcggggaa aaaagccgtc
     5761 cgccgccaaa acctttcatc acggtgagag caacaccgca ctgctcgcac agccggtaca
     5821 gttcctcgcg ttccggtgca atgccgccga gggactcatc gtaggtctct gcaaagtagg
     5881 tgttgacgtc ctcggttgca gggagaagat caaaggcagg attgatgctg aacaaaatca
     5941 cctcgatctc gccggattgt gcggccatct ttgcaacagc aggattgtgc gtactcatcc
     6001 ccagatggcg gataacacct gcttttttca gactgcgggc gtaggcaata aactcccctt
     6061 ccatgacact gcggtaatcc gccgcctcat ccacgtaatg aatcataccg agatcgatgt
     6121 gatcggtctg catccgcacc agcagatcgt caaaagcctc ccggaccttc tccatatccc
     6181 ggctgcggac atactgacca tcctgccagg ttgaaccgag atgtccctga atgatccagt
     6241 ccgctctcct gccggatatc gcagcaccga gattactgcg gacgtttggt tctgacatcc
     6301 agcagtccac aatgttgatc ccgcagtcag ccgcaagatc cacaactgct ttcacctcat
     6361 ctgcactgcg tccctgcagc cattctgctc caaggcctat ctcgcttacc aaaaggccgg
     6421 tttttccaag atgccgatac ctcatatttt ctcacacagg atccgtttgt ttgctcacct
     6481 atacgtttgc ggatacagct aatataggat gcaggaacag aatgctgccc tccctacagc
     6541 acctacatca tctcttcccg tggacccgac gacactttgg tcacaattca tatataatgt
     6601 tcccgtcgat actcagatat cggcttacgg agtggaacaa tctcatggaa agaaaaagca
     6661 tcctcatacc tcttgcggtt gtcctcatcc ttctcagctg ctttgtcggt ggtcagtatt
     6721 tctttgcaca ctccaattc
//
LOCUS       DALUAJ010000022         2705 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_4534,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000022 DALUAJ010000000
VERSION     DALUAJ010000022.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4534"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="O0X37_00400"
     CDS             <1..544
                     /locus_tag="O0X37_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJK61419.1"
                     /translation="REGETRGLGSKNRHEDLERAYTTIINTFSKMNIPKTVELCSTEP
                     KGMHGLFPVIDDVSFGGMEFEIWESLGGHIAGQLFLYEKNEGLLFTSDALINFASMTK
                     ARKDYCSIADYLVTSVNVNSAVARTERQELMRIAKEIDADLKKKGKRLLLCCGHGAIS
                     MLDDAGNMVSACEPIHYTAQ"
     gene            747..1430
                     /locus_tag="O0X37_00405"
     CDS             747..1430
                     /locus_tag="O0X37_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HJK61420.1"
                     /translation="MQKPLLEPSYTAVLFDMDNTLFDFIAAMKRGAAAAAETIGEGTG
                     DQLFSYYLRWKYHIEDHANLQDFMLDHHKFTVDGYLAAVKAFEDAKFSGLAPYPGIPD
                     LLASLKEKGYRLGIVTDAYEYAATERLRHTGLAEYFDTIVAYDSTGYKKPHHAPFEYA
                     MSLLGTLPHETVYVGDSIRRDIEPAKALGIAAVYVAYGDRNFFEQNRSCPPRVIVARS
                     PEDIGKILL"
     gene            complement(1438..2160)
                     /locus_tag="O0X37_00410"
     CDS             complement(1438..2160)
                     /locus_tag="O0X37_00410"
                     /inference="COORDINATES: protein motif:HMM:NF040371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6293 family protein"
                     /protein_id="HJK61421.1"
                     /translation="MPTTHVVFVGPERDFLMGSISLLREVGFSRIVLVTGNDPKFSGE
                     ALVQKTTKALSRDLGIFWPVTIVEVSKSSVMDAANQILTLINDERALGNDVLLNVAGA
                     LQPLAMSAYIAASMSRVRVVSAHPQFSDDGAIVGATEIVEIPVLPIGYPGSEQQLLIS
                     RIGDGVESLDSLIYLMFPEIDKNSKRFRSERSRLSHHLSKLEAGGFIVKTKYGRNIAI
                     HLTCLGQMVAQVISRGDVPQDN"
     gene            complement(2268..>2705)
                     /locus_tag="O0X37_00415"
     CDS             complement(2268..>2705)
                     /locus_tag="O0X37_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HJK61422.1"
                     /translation="AQVIDRNGWLHSGDLGTMDAEGYIKMVGRLKEMVIRGGENLYPR
                     EIEEFLHLHPKISDVYVIGVPDVKYGEELMAWVKVEPGQTLTEEEIKEFCNGKIARHK
                     VPRYYKFVDSFPMTVTGKIQKGEMQKISIAELGLEEVAKIQTA"
BASE COUNT          728 a          743 c          640 g          594 t
ORIGIN      
        1 ccgggagggg gaaacacgag gattgggatc caaaaaccgc cacgaagatc tggaacgtgc
       61 ctataccacg atcatcaaca ccttctccaa aatgaatatc cccaaaaccg tggagctgtg
      121 cagtaccgaa ccaaaaggaa tgcacggtct gttcccggtg atcgatgatg tgtcgttcgg
      181 cggcatggag tttgagatct gggaaagcct cggcggccac attgcaggac agctgttcct
      241 gtacgaaaaa aatgaaggac tgctcttcac cagcgacgca ctgatcaact tcgccagtat
      301 gacaaaagcc cgtaaggact actgttccat tgcggattat cttgtcacca gtgtaaacgt
      361 caacagtgcg gttgcccgga ccgaacgtca ggaactgatg cggattgcaa aagagatcga
      421 tgccgacctc aagaaaaaag gaaaacggct tctgctctgc tgcgggcacg gtgcaatctc
      481 aatgctggat gatgcaggaa acatggtttc cgcctgtgaa ccgatccact acaccgcaca
      541 gtaacccgga tctcacacca cataccaaaa tcactttttt cttacagagt tctcgcaaac
      601 aaaagaacag atacaccaca gaatcatatt cccaaaaaca gtgaatgaat ctgcccgcgg
      661 tttattgaca gaaaaaaccc ggaaagccct tcggtgccgg aaccggcatc cctaagtagc
      721 ctcccgaaaa acaactatca gcgcatatgc aaaaacccct tctggaaccc tcctacactg
      781 ctgttctgtt cgacatggac aacacgctct ttgactttat cgccgcaatg aaacggggag
      841 cagccgcagc agcagaaacc atcggggaag gaacaggaga ccagttgttt tcgtactatc
      901 tgcgatggaa ataccacatt gaagaccatg caaaccttca ggacttcatg ctggatcacc
      961 acaaattcac cgtggacgga taccttgccg cagtgaaagc atttgaggat gcaaaatttt
     1021 ccggtcttgc cccatacccg gggatccccg acctcctcgc ctctctaaag gaaaaagggt
     1081 accggctcgg cattgtaaca gacgcctacg agtacgccgc aaccgagcgc ctcagacaca
     1141 caggtcttgc agagtacttc gataccatcg ttgcatacga ctccaccggc tacaaaaagc
     1201 cgcaccacgc accctttgag tacgccatga gcctgttagg cacactgccg catgaaacgg
     1261 tgtatgtcgg agactccatc cgccgagaca tcgaaccggc aaaagctctt ggcattgctg
     1321 cggtctatgt agcgtacggt gatcggaact tctttgagca gaacagaagc tgcccgccgc
     1381 gagtcatcgt agcccgcagc ccggaagata tcggaaaaat tctcttataa aaaagattta
     1441 gttatcctgc ggcacatcac cgcgggagat cacctgggca accatctgcc cgagacaggt
     1501 cagatgaatg gcaatgtttc tgccgtattt ggttttcaca ataaatccgc ctgcctcaag
     1561 cttggagaga tggtgagaca gtctgcttct ttcactgcgg aacctcttcg agttcttgtc
     1621 aatttccggg aacatcagat agatcagtga atcgagtgac tcaacaccgt caccgatacg
     1681 ggagatcaaa agctgctgct ccgaacccgg gtagccgatc ggcagcaccg gaatctccac
     1741 aatctccgtt gcaccaacga tagccccgtc atcactgaac tgggggtgag ccgaaacgac
     1801 acgaaccctg ctcatgcttg ctgcaatgta ggcactcatc gcaagaggct gaagagcacc
     1861 ggcaacattc agaagaacat cgtttccaag cgcacgctca tcattgataa gcgtaagaat
     1921 ctggtttgcc gcatccatca ccgaggattt gctgacctca acgatcgtta ccggccagaa
     1981 gataccaaga tcccgggaaa gcgctttggt ggtcttctgg accagtgcct caccggagaa
     2041 cttcggatca tttcctgtta ccagtacaat acgggaaaat cccacttcgc ggagaagtga
     2101 gatcgaaccc atcaggaagt cacgctccgg tccgacgaaa actacgtgtg ttgttggcat
     2161 gttttaattc atgtttatat ttgccatgaa tattatatta gagtattggg aagatacagt
     2221 gtgagatata ctgtatccaa gtttattttt aaaaaaaaga ggacaagtta agcggtctga
     2281 atctttgcaa cctcttcaag accgagctca gcaatggaga tcttctgcat ctcacccttc
     2341 tgtattttgc cggtaacggt catcgggaag gagtccacga acttgtagta ccggggaacc
     2401 ttgtgacggg cgatcttgcc gttgcagaac tccttgatct cctcttcggt aagtgtctgt
     2461 cccggctcga ccttgaccca tgccatcagt tcctcgccgt acttaacgtc cgggacaccg
     2521 atcacataca cgtccgagat cttcggatgc aggtgcagga actcctcaat ctcacgcgga
     2581 tagaggttct caccgccgcg gatgaccatc tcctttaacc tgccgaccat cttgatgtaa
     2641 ccttcggcat ccatcgtgcc aagatcaccc gagtgcagcc atccgttgcg gtcaatgacc
     2701 tgggc
//
LOCUS       DALUAJ010000023         2137 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_4606,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000023 DALUAJ010000000
VERSION     DALUAJ010000023.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4606"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="O0X37_00420"
     CDS             <1..690
                     /locus_tag="O0X37_00420"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HJK61423.1"
                     /translation="HMTEDAISPYYQPTLRVRLMATGGILAVFGVVLCFFFLTMPSDV
                     FYKFIGFLGLYLMPGFGKESIVPLAMAFGISWWQITLGIVVGDMALAIIIAYNFDLLL
                     RLPLLGRVLRYFTTKTNEVLQKYLWIKGLSLVGLFLFMYIPFMGSSAINTTIVGRLLS
                     VPPKTLLTIVFVGSILATLTMAVGMQAILELWAANPVYAVVAVIAVVIVAVACWKLWQ
                     KYTEKRFGKGQ"
     gene            complement(735..1175)
                     /locus_tag="O0X37_00425"
     CDS             complement(735..1175)
                     /locus_tag="O0X37_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HJK61424.1"
                     /translation="MPQTAHCKRLTMKEEDYLEAILNVSKEKGYAKTRDVADELGLSP
                     PSVVEMFAKLDRKKLVVYRKYEGVTLTEAGRTIAEQIKYRHDVLVGFLRLISVPEEIA
                     TKDACFMEHELNAETIQKIKQFVEYVSISKSAQIEMKRFTESCK"
     gene            1335..1811
                     /locus_tag="O0X37_00430"
     CDS             1335..1811
                     /locus_tag="O0X37_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HJK61425.1"
                     /translation="MQTTSQSVALNCLPCGSEATVVSLHHGAEITKRLLELGVTRGTH
                     LTVLGKAPLGDPLFVRVRGCQIAMRKAEAENISVVKDGIWTLDAAPVGHTVRVTEVRP
                     NTEITSRLLELGVTKGSSIRITGAAPLGDPMTISVRGCQFAIRRHEAENIQVELES"
     gene            1808..>2137
                     /locus_tag="O0X37_00435"
     CDS             1808..>2137
                     /locus_tag="O0X37_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HJK61426.1"
                     /translation="MSEKRVVVALAGNPNCGKTTLFNNLTGMRQHVGNWPGKTVAIER
                     KDGKATYDGTTLEIVDLPGTYSLSSRSLEEEIAVEYLLTEKPDVVVNIIDAAHLERNL
                     YLTLQLIE"
BASE COUNT          470 a          487 c          560 g          620 t
ORIGIN      
        1 catatgacgg aagatgcgat atcaccctat tatcagccga ccctccgcgt acggcttatg
       61 gcaaccggag gaattctggc agtgtttggt gtggtgcttt gtttcttctt cttgacgatg
      121 ccctccgatg ttttttataa gttcatcggg tttctgggac tgtatctgat gccagggttt
      181 gggaaggaga gtattgttcc tcttgcaatg gcattcggga tttcctggtg gcagattact
      241 cttggtattg ttgtcggcga tatggcactt gccatcatta ttgcctacaa ctttgatctt
      301 ctgttgcgtc tgccccttct tggccgggtt cttcgttact tcacgaccaa gacgaatgag
      361 gtactgcaga aatatttgtg gattaaaggt ctgtcgcttg tcgggctgtt tctgtttatg
      421 tacatcccgt tcatgggctc aagtgcgatc aacacaacga ttgtggggag actgctttcc
      481 gtaccgccaa aaactcttct gactattgtt tttgtcggaa gtattcttgc aaccctgacg
      541 atggcagttg gtatgcaggc aatccttgag ttgtgggctg ccaatcctgt gtatgccgtt
      601 gttgcggtga ttgctgtggt catagttgcc gttgcctgct ggaagctctg gcagaagtac
      661 accgagaagc ggtttggaaa gggtcagtaa tccggatttt cacgcatcct tttggatggt
      721 gcccggtaaa aaaattattt gcaggattcc gtgaatcgtt tcatctctat ctgtgcggat
      781 ttggagatgc tgacgtattc tacgaactgt tttattttct gaatggtttc tgcgttgagt
      841 tcgtgttcca tgaaacaggc atctttggtt gcaatctcct caggaacgga gatcagcctg
      901 agaaacccta ccagtacatc gtggcggtac ttgatctgct cggcgatggt tcttcctgcc
      961 tctgtgagtg ttactccttc atatttccgg tacacaacca gtttcttccg atcgagcttt
     1021 gcaaacatct ccacaaccga gggcggcgaa agccccagct cgtctgcaac atcccgggtc
     1081 tttgcgtaac ctttctcctt tgagacgttg aggattgctt caaggtaatc ctcttctttc
     1141 atggtgaggc gtttgcagtg tgctgtctgc ggcattgcat ccatgtgata tatcattcat
     1201 catacgagat tatgagcgtt tgctttagaa attttcggat attttgaaaa aatccgaaac
     1261 atcttcggcc aggttgtgcg atccaaaaat tgttaggggg caggatatac gctcctaaca
     1321 ggatacactt gtgtatgcaa acgacatctc aatcagttgc cctgaactgc cttccctgcg
     1381 gttctgaggc aacggtagtg tcgctccatc acggggctga gattaccaag cgtttacttg
     1441 agctgggtgt tacccgggga acgcatctga cggttctcgg gaaagcaccg ctcggggatc
     1501 ccctgtttgt acgtgtccgc ggttgtcaga tcgcgatgcg aaaagccgag gccgaaaata
     1561 tatccgtggt aaaagacggg atctggacgc ttgatgctgc gcccgtaggt cataccgtcc
     1621 gcgttacgga ggtccggcca aacactgaga ttacttcccg tctccttgaa ctgggtgtga
     1681 cgaaaggaag cagtatccgt atcactggtg ctgctccgct tggagatccg atgacaatct
     1741 cagttcgcgg atgtcagttt gcaatccgcc gtcatgaagc agaaaatatt caggtggaat
     1801 tagagtcatg agtgaaaaac gtgtagtggt tgctcttgcc ggaaatccaa actgcggtaa
     1861 gacgacactg ttcaataatc tgaccggcat gcgtcagcat gtgggtaact ggccgggaaa
     1921 gaccgttgcc atagagcgaa aagacggtaa ggcaacctat gatggtacca cgcttgagat
     1981 cgtggatctt ccgggaacct acagtctgag cagcagatcc cttgaagagg agattgctgt
     2041 tgagtatctg cttaccgaaa aaccggatgt cgtcgtcaat attattgatg ctgcgcatct
     2101 tgagcggaat ctctatctga cactccagtt aattgag
//
LOCUS       DALUAJ010000024         1802 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_4623,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000024 DALUAJ010000000
VERSION     DALUAJ010000024.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4623"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(51..389)
                     /locus_tag="O0X37_00440"
     CDS             complement(51..389)
                     /locus_tag="O0X37_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HJK61427.1"
                     /translation="MIGTLVTGFGIWNPLIWIVVIAVALLFSWLLWRCGEEDYEEDAE
                     TTAPYLSGNAEPEKGAVHIRAGNMYWGFTKALSAYYDRLIPLHSGIVNDYVSWFLIGV
                     VILFVVVILV"
     gene            complement(386..>1802)
                     /locus_tag="O0X37_00445"
     CDS             complement(386..>1802)
                     /locus_tag="O0X37_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HJK61428.1"
                     /translation="AAQVYAHGAVVYVFGAAPGTGAVPVDSGGIPVRILFNIDGFGAF
                     MLVSAAIVSFAVILYLTVSQSKRSSRSEFYALYLLLVAGIFGMVSTGDMFNFFVFLEI
                     NSLAASALIAYHRNGGLAAEGALKYLIVNTVGGLMILFAIGLLYAQYNSLNMAVIACN
                     LSLTTLNLLALILFIAGLATKAGAVPFHFATPDAYSVAPSGVTALMIVASQAGLYGMF
                     RILFSVYGGVFTSATVGWIVIILGVLSMFIGVTMAIPQKDIKRLLSYHAVSQTGYMLL
                     GVGVALAVLGDVTLTNAFGQMAMEGGLFHIINHAMYKGLLFLAVGAVIYRTGVWNLNE
                     MGGLGHKMKWTMIFFLIGALAIAGIPPFNGFASKLMIYESTFAFNPAIAIIAMVVSIL
                     TLASFMKVFHSVFMGPELPEYAGVREVPKRMLAGMAILAVFVIIFGIVPDLVVDTLIT
                     PAADALLHQWQYILSVLGGGL"
BASE COUNT          503 a          474 c          485 g          340 t
ORIGIN      
        1 gctgttgaag tggaacaccc agagggaccg gttcagcgat gttgtcagtt tcatacgagg
       61 atcaccacca caaagaggat cacaacgccg atcaggaacc aagagacgta atcgttcaca
      121 attcctgagt gcagcggaat caggcggtca tagtaggccg acagtgcctt ggtaaatccc
      181 cagtacatgt tgccggcacg gatgtggacc gcacctttct ccggttcggc gtttccggac
      241 agatacgggg cggtggtctc agcatcctct tcataatcct cctcgccgca tctccagagc
      301 agccaggaga agaggagcgc aaccgcaatt acgacaatcc agatcagcgg gttccaaatc
      361 ccaaatcctg tcacaagtgt accaatcata atccgcctcc aagaaccgag agaatatact
      421 gccactgatg cagcagggca tctgcggcag gtgtaatcag tgtatcaacc acaagatccg
      481 ggacaatacc gaagatgatc acaaacacgg caagaattgc cataccggca agcatccgct
      541 tcgggacctc acgcacgccc gcatactcgg ggagttccgg acccatgaag accgagtgga
      601 acaccttcat gaaggaggca agcgtgagga tgctcacaac cattgcgata atggcaattg
      661 ccggattgaa cgcaaacgtt gactcgtaga tcatcagttt tgaggcaaat ccattgaacg
      721 gcgggatacc tgcaattgca agagcaccta tcaggaagaa gatcatcgtc cacttcatct
      781 tatgaccgag acctcccatc tcgtttaagt tccagacgcc cgtacggtaa atgaccgcac
      841 caaccgcaag gaacaacagt cccttgtaca tcgcgtggtt aatgatatgg aaaagacctc
      901 cttccattgc catctggccg aacgcattgg taagagttac gtcgccgagc actgcaaggg
      961 caacaccgac accaagcagc atgtaaccgg tctgcgaaac cgcatggtac gacagtagcc
     1021 gtttgatgtc tttctgcggg attgccatgg taacgccgat gaacatggag aggacaccaa
     1081 ggatgatgac aatccatccg acggtcgcag aggtgaagac accgccgtac accgagaaca
     1141 gaatacggaa cattccgtat agacctgcct ggcttgcaac gatcatcaga gcagttaccc
     1201 cggacggtgc aaccgagtag gcatcaggcg ttgcaaagtg gaacggtact gctcccgcct
     1261 tggttgcaag tcctgcaatg aagaggatga gtgcaagaag gttcagtgtg gttagagaca
     1321 gattgcaggc gatgaccgcc atgttgagcg agttgtactg cgcatacagc aggccgatcg
     1381 caaacagaat catcagaccg ccaacggtgt tgacgattaa atacttcagt gcgccttctg
     1441 ctgcgaggcc gccgtttctg tgataggcga ttaacgcgga tgcagcaagc gagttgatct
     1501 caaggaagac gaagaagttg aacatgtctc cggttgagac catgccgaaa atacctgcaa
     1561 caagcaggag atagagggca taaaactcag aacgggagct gcgtttgctc tgggagacgg
     1621 tgagatagag gatcaccgca aacgatacga tcgctgcgga gacgagcatg aaggcaccaa
     1681 acccgtcgat gttgaagaga atgcgaacgg gaattccgcc ggagtcaacc ggtactgcac
     1741 cggttccggg agcagctcca aagacgtaga cgactgcacc gtgtgcatac acctgtgctg
     1801 ca
//
LOCUS       DALUAJ010000025         2200 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_4712,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000025 DALUAJ010000000
VERSION     DALUAJ010000025.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4712"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            6..1838
                     /locus_tag="O0X37_00450"
     CDS             6..1838
                     /locus_tag="O0X37_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HJK61429.1"
                     /translation="MPLPQSPLPQPEFLPMTLKELNACGITQPDIILVSGDAYVDHPS
                     FAAALIGRALWDAGFTVGIIAQPDWRDHKFKSFTQLGEPRLFFAVVPGAVDPLVNAYT
                     PALKKRSKDSYSPGGKLTRPERTTIVYVNALHRQYPKTPIIIGGIEASLRRFAHYDYW
                     SNSVRQGILADAPASLLVYGMGELALIEIAKRMRNGDPAERLRDIPGTCWTMSVGEFR
                     KNPLDDAVLLPSYPDVIDKRKFANAYAVMYRDGHKQLVQPHPKTVIIANPPMRPMTTD
                     ELDHLYELPFTRRQHPSYQEPVPALEPVQFSLVTHRGCFGGCSFCAITHHQGRIITSR
                     SEESLIREAKRITEMPEFRGTIQDVGGPTANMYGCSCKIWETGTPCPDKNCMKCGKFT
                     NGTERQLALLERLRKLPKVKHVFISSGIRYDLIPKDDQGDRYLLELREHYISGHLKVA
                     PEHISTHVTELMNKPSGRVFSDFYTRFEQLQTRETSTGKNRQYLLPYLMSSHPGCRIE
                     DMIDLALYLQKYHLYTEQVQDFTPVPMTLSTAMYYSGIHPLTQKKVYVPVGGEKRIQR
                     ALLQWKDPKQYDLAIAGLIQAKRSDLIGVLIPAAHGRSFKEKKR"
     gene            complement(1911..>2200)
                     /locus_tag="O0X37_00455"
     CDS             complement(1911..>2200)
                     /locus_tag="O0X37_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HJK61430.1"
                     /translation="ILGMSTRDSLIIGFGMAPRGEVAMIVGLIALTHFQEMAEAATDV
                     VQKEYLLQLGNEVFIAIVLMSLVTTVIVPLVYRGWFFKAEKKPDPAVGIQQ"
BASE COUNT          560 a          570 c          545 g          525 t
ORIGIN      
        1 caactatgcc tctccctcaa agtcccttac cgcagccgga atttttaccg atgacgctaa
       61 aggaactgaa tgcgtgtggt attacccaac ccgatataat tctcgtttcc ggtgatgcct
      121 acgtagatca tccttcgttt gcggcggcat tgatcggtcg ggctttgtgg gatgcgggtt
      181 ttactgtagg gatcatagcc cagccggact ggagagatca caagttcaaa agttttacac
      241 agctgggaga accgcgcctg ttttttgcgg tcgttccggg tgcggtggat cctctggtca
      301 atgcgtacac gccagcgctg aaaaaacgat caaaggacag ttactcaccc ggaggaaaac
      361 tcacccgccc agagcggaca acaattgtgt atgtaaacgc tctccaccgg cagtatccta
      421 agaccccgat catcattgga ggcattgagg caagcctccg gcgttttgcg cactatgatt
      481 actggagcaa cagtgtccgg cagggaatcc ttgctgatgc accggcatcg cttctcgtgt
      541 atggaatggg agaacttgct ctcatcgaaa ttgcaaaacg aatgcggaat ggagatcccg
      601 cagagagact tcgtgatatt cccggtacct gctggacaat gagtgtgggc gagttccgca
      661 aaaatccgct ggatgatgcc gtactgctcc cgtcctatcc ggatgttata gataaaagga
      721 agtttgcaaa cgcctatgca gtcatgtatc gtgatgggca caagcaacta gtacagcctc
      781 atccgaaaac cgtgatcata gcaaaccctc ccatgaggcc gatgaccacc gatgaactgg
      841 atcatctcta tgaactcccg tttacccgca ggcagcatcc ctcgtatcag gaaccagttc
      901 ccgctcttga gcctgttcag ttctccttgg taactcaccg cggctgcttt ggcggctgtt
      961 cgttctgcgc catcacgcat catcagggaa ggattatcac ctctcggagc gaggagtcgc
     1021 ttattcgtga agcaaagcgg ataaccgaaa tgccggagtt ccggggaaca attcaggacg
     1081 ttggtggacc aaccgcaaac atgtacggtt gttcctgcaa aatatgggag acgggaacgc
     1141 cctgccctga caaaaactgt atgaaatgcg gaaagttcac caacgggacc gaacggcagc
     1201 tggcacttct ggaacgtctg cgtaaacttc caaaggtcaa acatgtgttc atcagctccg
     1261 gcatccgcta tgatctcatt ccaaaggatg atcagggaga ccgatacctg ctggaattac
     1321 gggaacatta tatctccgga catctcaagg tcgcacccga acacatctcc acgcatgtga
     1381 ccgaactgat gaacaaaccg tctggccgtg tgttttctga cttttatacg cgttttgagc
     1441 agttacaaac gcgcgaaaca tcgacgggaa agaaccgtca gtatttgctg ccgtatctca
     1501 tgtcctcaca tcccgggtgc cggattgagg atatgattga tcttgcactg tacctgcaaa
     1561 agtaccatct ttacactgag caggtacagg actttacacc ggttccgatg actttgtcca
     1621 cggcaatgta ctacagcgga attcaccctc ttacgcagaa gaaggtctat gttccggttg
     1681 ggggtgaaaa acgtattcag cgtgcccttt tgcagtggaa agatcccaag cagtatgatc
     1741 ttgccattgc aggactgata caggcgaaaa gaagtgatct cattggcgtt ttgattcctg
     1801 ctgcacacgg gcgatctttc aaagaaaaga agagataaaa taacgctcgt tgggatgttg
     1861 tcagtaggac tctactgcaa cttcaaaaac ttctgttgga aaaaaatgtt ttattgctgt
     1921 attcccacag ctggatccgg ttttttctcg gccttgaaga accatccccg atagaccaag
     1981 ggcacaatca cagttgtaac aagcgacatc agcacaattg cgatgaaaac ctcgttaccc
     2041 agctgaagga ggtactcttt ctggaccaca tcagttgccg cttcggccat ctcctggaag
     2101 tgtgtcagtg cgatcagccc gacgatcatt gccacttctc cgcgtggcgc cataccaaat
     2161 ccgatgatca gcgagtcgcg ggtgctcatg ccaagtatcc
//
LOCUS       DALUAJ010000026         2709 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_4850,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000026 DALUAJ010000000
VERSION     DALUAJ010000026.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4850"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(122..340)
                     /locus_tag="O0X37_00460"
     CDS             complement(122..340)
                     /locus_tag="O0X37_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61431.1"
                     /translation="MIPDFRDESCTYLMLRLKVALKKEKGAFSFLGTSTQVKTVEGAF
                     PKSGYAVDVITQDEKDTYLITLTPSVQK"
     gene            complement(359..583)
                     /locus_tag="O0X37_00465"
     CDS             complement(359..583)
                     /locus_tag="O0X37_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HJK61432.1"
                     /translation="MTNKLDITGKVCPFCLLAVDKELKKLPSGAELVVVCDHAPAATA
                     SIPEYCGNRNIKCSVKLIENGLWEITITKN"
     gene            complement(596..1117)
                     /locus_tag="O0X37_00470"
     CDS             complement(596..1117)
                     /locus_tag="O0X37_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HJK61433.1"
                     /translation="MVDGFLIPKETAVLLVPIATLILGLVIGWLGQRSGFCSIGGIRD
                     YLLFRQTRLLKGYVALIISAFVFYLIFSLLVPAAIPSFPWCIQDGQLLTAIGGAPAVS
                     AAGVIILMIIGGIFIGLIGTLLGGCPLRQLVMTSEGNIKSLVFVVGMLVGAVIFTAVV
                     SPWIIEVFTAIGL"
     gene            complement(1120..1650)
                     /locus_tag="O0X37_00475"
     CDS             complement(1120..1650)
                     /locus_tag="O0X37_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HJK61434.1"
                     /translation="MVANSSDEKKGLDSYLKMPLVGGAVIGFAAALIQALLFAAGGPQ
                     AYGFCVACHSRDLINYVYNSLTGSNLFLAPFSANAVAAGTLPVLTIIGVLIGAVAAAV
                     LYKEFRVKKGDAKGYVAYGIGGILFMIFALCMGACPYRLALRIGYGDLVAVFGLIAII
                     IGVLIGIKIALKRMEA"
     gene            complement(1815..2489)
                     /locus_tag="O0X37_00480"
     CDS             complement(1815..2489)
                     /locus_tag="O0X37_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HJK61435.1"
                     /translation="MNQPINETPVQIIINGRAAMTIMTSAEDPTDLVVGQLYTERAIE
                     TYADISSMHTDGPQTSVVTTNPFEILLSRKTVLAGCGGASSFLDSGRLGNITSDLAVS
                     AEQIQSSAAHLPETCWHSGALFTEEGRLLVAVTDLTSQNVADQIIGWGLAHSTIFEKT
                     YLLISGNIVAETVRKAVIAKIPLLITDAEITTAAVAAADDAGMTLCKLVEDTVIPHTR
                     SNHCIL"
     gene            complement(2492..>2709)
                     /locus_tag="O0X37_00485"
     CDS             complement(2492..>2709)
                     /locus_tag="O0X37_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HJK61436.1"
                     /translation="TVDGVHPHEVAYLLDEAAGIMVRSGEHCCQPLMHALCLNGGTVR
                     ASAYCYNTEDDVDLLIATTEEIAGMVK"
BASE COUNT          714 a          610 c          633 g          752 t
ORIGIN      
        1 catcgaactg ccaggttcca gtcatcctgg tcgtgaacag cgcttcatta tcgtcagcct
       61 taatttcggc ttcgtactgc tttacctgtg cgagatcaat attgttcaac atgtttctgt
      121 ctcatttttg tacggaaggt gttaatgtaa tgagatatgt gtctttctca tcctgagtga
      181 ttacatcaac agcatatcct gattttggaa atgctccttc cactgttttt acctgtgttg
      241 atgtcccgag aaagctgaat gctccttttt cttttttcag ggctactttc agcctcagca
      301 tcagataggt acacgattca tcacggaagt cagggatcat cctaactcat ttggtttctc
      361 agttcttagt gatagtgatt tcccaaagtc cgttttcaat cagtttgaca ctgcacttga
      421 tgtttctgtt tccacaatat tcgggaattg acgctgttgc tgcgggtgca tggtcacaca
      481 caacaacgag ttctgcgccc gatgggagtt ttttcaactc tttatcaact gccaaaagac
      541 agaacggaca tactttcccg gtaatatcta atttgttagt catggtttgt tcacctcaga
      601 ggccgattgc tgtgaagact tcaatgatcc agggggagac gactgctgtg aagataactg
      661 ctccaacaag catgccaact acgaatacca gggacttgat gtttccttca gaggtcatga
      721 caagctggcg gagtggacat cctccgagaa gcgttccaat gagtcctatg aagataccgc
      781 caatgatcat gaggatgatt acacctgctg cactaacagc tggcgctccg ccaattgcag
      841 tgagtaactg tccatcctgg atacaccagg ggaaacttgg aattgccgcg gggacaagca
      901 gtgaaaagat caagtagaag acaaatgcag agataatgag tgctacatat cctttcagaa
      961 gacgtgtctg ccggaagagc agataatcac ggatacctcc aatagaacag aatccgcttc
     1021 tctgaccgag ccacccaatt acaagaccca gaataagagt tgcgattgga acaaggagga
     1081 ctgcggtttc tttgggaatt aaaaatccgt caaccattat tatgcctcca ttctcttcag
     1141 ggcgatctta ataccgatga ggacaccgat aatgattgca ataagtccaa agactgcaac
     1201 gaggtcaccg tagccgatac gaagtgcaag gcggtatggg catgctccca tacagagggc
     1261 aaagatcata aagaggatac cgccgattcc atatgctaca taacctttcg catcaccttt
     1321 cttgacacgg aactctttgt agagaactgc tgcagcaact gcaccaataa gaacaccgat
     1381 gatggttaag acaggcagtg ttcctgcagc aaccgcattt gcagagaatg gtgcaaggaa
     1441 aaggttgctt ccggtgagag agttgtacac atagttgatc aagtcacgac tgtgacatgc
     1501 aacacaaaaa ccgtaagcct gcggtccacc tgcggcaaac aggagagctt ggataagtgc
     1561 tgcggcaaag ccaatcacgg ctccaccaac gagcggcatc ttaagataag agtccagccc
     1621 ttttttttca tcagaactat tagctaccat agctgtattt cctattcgaa tggatgcaat
     1681 atcaatgttg gtagaaatga ttttatagtt ccatgtcttt ggacatttca ctttgttatt
     1741 gttaataaaa ctatcacaac taatttatat tgactcgcgg ttctggcttt agcctgcctg
     1801 aaataaatag atggttagag aatacagtga ttggatctgg tatgagggat aacggtatcc
     1861 tccacgagct tacagagtgt catccctgca tcatccgcag ctgcaactgc cgctgttgtt
     1921 atctctgcat ctgttatgag aagaggtatt tttgcaataa ctgcttttct caccgtttct
     1981 gcaacaatat ttcctgagat aagtaggtac gttttttcaa aaattgtgct gtgtgcaagt
     2041 ccccatccta taatttggtc tgccacattc tgggaggtga gatcagttac tgcaacaagg
     2101 agtcttccct cctctgtgaa taatgcaccg ctgtgccagc aggtttctgg gagatgtgca
     2161 gcagacgatt gtatctgttc tgctgatact gcaagatcag aggtaatgtt tccaagtctg
     2221 cctgagtcaa ggaaagagga cgctcctcca catcctgcaa gaacagtttt gcgggaaagc
     2281 aggatttcga atggatttgt ggtgacaaca cttgtctgag ggccgtccgt gtgcattgaa
     2341 ctgatatctg catatgtttc aatagcacgt tctgtgtaga gctgcccaac tacgagatct
     2401 gtcggatctt ctgcagaggt catgatggtc attgcggcgc ggccattaat gataatctgt
     2461 actggagttt cgtttattgg ttggttcatg attacttcac cattccggca atttcttcgg
     2521 ttgttgcaat aagcaggtca acatcgtctt cagtgttata gcagtaggca cttgcacgga
     2581 cggttcctcc gttcaggcag agggcgtgca tgagcggctg gcagcagtgt tcgccggacc
     2641 ggaccatgat gcctgcagcc tcatcaagga ggtaggcaac ttcatgggga tgtacaccgt
     2701 caacggtaa
//
LOCUS       DALUAJ010000027         5534 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_4857,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000027 DALUAJ010000000
VERSION     DALUAJ010000027.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4857"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            5..1261
                     /locus_tag="O0X37_00490"
     CDS             5..1261
                     /locus_tag="O0X37_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORC1-type DNA replication protein"
                     /protein_id="HJK61437.1"
                     /translation="MSDEPFKSTGLFKKYTIKNKIFQNREVLRHSYSPRELPHRVDQI
                     DSIAVILAPALQGATPSNILIYGKTGTGKTATVKFVGSELENESSEFSPCRMVHLNCE
                     TIDTQYRVLAQIANHISGHDLKPSDRAKNTIPATGWHTDQVYSELKNVLEQAGGLQII
                     VLDEIDKLVKKSGDDTLYNLTRINSDLKNARVSIIGISNDLTFKDFLDPRVLSSLSEE
                     ELVFPPYDAVQLQDILHQRAEMAFLPDAVSEDVIALCAALAAQEHGDARRALDLLRVS
                     GELAERESSDKVLESHVRRAQENIETDTMSECVQTLPRQGKMVLCAMLLVSSSGQKVF
                     TSGSVINIYRELARELDIEPLTHRRVSDLINELNMLGIVTARVVSHGRHGRTTEIYFN
                     SPTNQIRTVVMNDPSLRECSILQPLL"
     gene            1291..1782
                     /locus_tag="O0X37_00495"
     CDS             1291..1782
                     /locus_tag="O0X37_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HJK61438.1"
                     /translation="MDEKDTVILRELQKNSRISARDIAVMLEVDEREVEDRISALHAA
                     GVLRRCTAVIDYSAAGVDEVAVILSLKVTPERGLGYDGIAAQIARFPQVESILLLTGT
                     YDLQVIIRGQSMADVSRFVAEQIASIEGVRETMTQIVMRTYKEQGVEMSRPGSRDRQL
                     ITF"
     gene            1795..2958
                     /locus_tag="O0X37_00500"
     CDS             1795..2958
                     /locus_tag="O0X37_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJK61439.1"
                     /translation="MRSFVSRMAAEIPPSGIRKFFDLLLTMDNVISLGVGEPDFDTPW
                     NVSQAAISSIEKGMTMYTSNRGLAELRELLSADLDQRYHTKYCPETEMIVTTGVSEAL
                     DVAVRAVVDPGDEVLVVDPCFVSYQPEVLMAGGKPKALPCRAQDQFKVTPEALMESVT
                     PKTKVLMMNFPNNPTGGVMGADDLKAISDIVIDHDLVVISDEVYSELTYDGHHVSTAS
                     IDGLWDRTITLNGFSKAYAMTGWRLGYICAPKEICDAALKIHQYVMMSAPTAAQFAAI
                     EALKNGEEAKNEMVAEYRMRRNLFVKGLNDVGLPCHLPKGAFYAFPSIEGTGLTDEEF
                     AEQLLKEQHVAVVPGSAFGESGRGHVRTCYAVDRSKLSEAVRRIGTFVESLHS"
     gene            3015..4433
                     /locus_tag="O0X37_00505"
     CDS             3015..4433
                     /locus_tag="O0X37_00505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase II small subunit"
                     /protein_id="HJK61440.1"
                     /translation="MQKREIVARFLEAGLLVHPAVVTYISESGGSGIIEGIISSLPSS
                     VSVVTPKDVPGMTAARVDKLEVGVSALPEILAGRAGSSVPTATPEESFALFRDRYDAL
                     AAMMRPRVSPIPIEALVKQNNRFADTDIGAIGMVVESSTSAKGHRIVEIEDPTGTIKV
                     LFNKNREGFDEAEKIIPDEVIGVRGKLARDTGGGGGMIFSDTLFRPEIPLTHTISPSK
                     EPGKAVLISDVHVGSDTFLPDAWDRFASWLESTPEVKYLLIAGDVVDGIGVYPNQDKE
                     LHIKTIYEQYDAVGEMLSSLPSHLQIVLSPGNHDAVRAAEPQPVLPEEFRTRFPSNVT
                     FVENPAVVNIQSVNVLMYHGRSIDDLIKFIPGASYDHPGEIMEAMLKRRHLGPIYGQR
                     TPLLSTPTDHLVIRDVPDILHTGHVHITDVISYRGVLGVNSGTWQGQTSFQKQMNINP
                     TPGEAIVVDLFTMGYEKMCFHT"
     gene            4607..>5534
                     /locus_tag="O0X37_00510"
     CDS             4607..>5534
                     /locus_tag="O0X37_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HJK61441.1"
                     /translation="MDLIYLAPICAVIGLLFALYSYSVVKKEGTGDEKMQKISAAIHL
                     GAMVFLNRQYRAIGAFVVVIAIILAGLSFVTDDSMSIWAAPAYVIGAALSSLAGYIGM
                     HSATKANVRTTNAAKRGIADAVKVSFASGSVMGMSVVGLGLLGASVVFLVLIGIFGGF
                     GDIVAVNTVVSNMAAFGFGASSVALFARVGGGIFTKAADVGADLVGKVEAGIPEDDPR
                     NPAVVADNVGDNVGDVAGMGADLFESYVGSIIASMALGIVGATFALEQFGENASALNA
                     ILLPMLIAAFGIIAAAIGTLFVRTKKNENSAIH"
BASE COUNT         1264 a         1347 c         1453 g         1470 t
ORIGIN      
        1 tatcatgtct gatgaacctt tcaaatctac tggtcttttc aaaaaatata ccattaaaaa
       61 taaaatattt caaaatcgtg aagttcttcg ccacagttac agtccgcgtg aactcccgca
      121 ccgtgttgat cagattgatt ccattgctgt cattctcgcc ccagctctcc agggtgcaac
      181 tccttctaac attctgattt atggaaaaac cggtactgga aaaactgcaa ccgtcaaatt
      241 tgttggatcc gagctggaga atgaaagttc cgagttctct ccatgccgta tggttcatct
      301 caattgtgaa accattgaca ctcagtaccg tgtactcgca caaatcgcca atcacatcag
      361 cggccatgat ctcaaaccaa gtgatcgcgc caagaacacg attcctgcaa ccggctggca
      421 caccgatcag gtgtactccg aactcaagaa tgttcttgaa caggccggag gtttacagat
      481 catcgtactt gatgagattg acaaactcgt gaagaaaagc ggtgatgaca cgctttacaa
      541 tctgacgcgg atcaacagtg acctcaaaaa tgcccgtgtc agtatcatcg gtatatccaa
      601 tgatcttacc tttaaggact ttcttgaccc gagagtcctt tcctctctct ctgaagagga
      661 acttgtgttc cctccttatg atgccgttca gttgcaggat attttacacc agcgtgccga
      721 aatggccttc ctccctgacg ctgtcagtga agatgtcatt gctctttgtg cagcacttgc
      781 tgcccaggag catggtgatg ccagacgtgc acttgatctt cttcgtgtct ccggggaact
      841 tgccgagcgc gagtcctcgg acaaagttct tgagtcccat gttcgccggg cccaggagaa
      901 catcgaaacc gataccatga gtgagtgtgt tcagactctt cctcgtcagg ggaaaatggt
      961 actgtgtgcc atgcttcttg tctcctcctc aggccagaaa gtgtttacca gcggtagcgt
     1021 catcaacatc taccgtgaac ttgccagaga gcttgacatc gaaccgctga cacacaggcg
     1081 ggtttcggat ctcattaacg aactaaatat gctgggtatc gttaccgccc gggtcgtatc
     1141 acacggacgc catggcagga caacagaaat ctatttcaac agtcccacta atcagatacg
     1201 tacagtagta atgaacgatc caagtcttag ggagtgcagt atcttacagc cgctcctctg
     1261 agacctctgc atcacacaag gtgaaaaatt atggacgaaa aggatacagt aatccttcgg
     1321 gaactgcaaa agaacagccg tatctctgca agagatattg ctgttatgct ggaggttgat
     1381 gaacgtgaag ttgaggatcg tatttcagca ctccacgcag caggtgtcct ccggcgctgt
     1441 actgcggtta tcgactacag tgctgccgga gttgacgagg tggctgtcat tctgtccctt
     1501 aaagttactc cggaacgcgg acttggttat gatggcattg ccgcccagat tgcccgcttc
     1561 ccccaggtgg agtctattct cctccttacc ggaacgtatg atcttcaggt tattatccgt
     1621 ggtcagtcaa tggccgacgt ctcccggttc gttgccgaac agattgcatc cattgagggc
     1681 gttcgagaaa cgatgacgca gattgtgatg cggacataca aagagcaggg tgtggagatg
     1741 tcgcggccgg gtagtcgcga ccgccagctc atcacgttct gaaggcgggg gatcatgcga
     1801 agttttgtat cacggatggc tgcggagatc cctccgtcgg gcattcggaa gttctttgat
     1861 ctgctgctca ccatggacaa tgtgatcagc cttggtgttg gcgaaccgga ttttgatact
     1921 ccttggaacg tctctcaggc agccatctcc tcaattgaaa aaggcatgac gatgtacacg
     1981 tcaaaccgcg gtcttgctga actgcgggag cttctttctg ccgatctgga tcagcgttat
     2041 catacaaaat actgtccgga gaccgagatg atcgtgacga ccggtgtttc cgaggcactt
     2101 gatgttgctg ttcgggcggt ggttgatccg ggtgatgagg ttcttgttgt ggacccctgt
     2161 tttgtctcat atcagcccga agtgctgatg gcaggcggaa aaccgaaggc tctcccctgc
     2221 cgggcacagg atcagtttaa ggtaacacct gaggccctga tggagtctgt tacaccgaag
     2281 acgaaagttc tgatgatgaa cttcccgaac aatcctaccg gcggagtaat gggtgctgat
     2341 gatctgaagg ccatttccga tattgtgatt gatcacgatc ttgttgtgat ctcagatgaa
     2401 gtgtactcgg agctgaccta tgacggtcat catgtctcta ctgcctcaat tgacggcctg
     2461 tgggatcgga caatcacgct gaacggtttt tccaaggcat atgctatgac tggatggcgg
     2521 cttggctaca tctgtgcgcc gaaggagatc tgcgatgccg cactgaagat tcatcagtat
     2581 gtgatgatgt ctgcaccgac tgctgcgcag tttgcagcaa ttgaggcact gaagaatggt
     2641 gaagaggcaa agaatgagat ggttgcagag taccggatgc ggcgcaacct ctttgtcaaa
     2701 ggcctcaatg atgtcggtct tccctgccat ctgccaaagg gtgcattcta tgctttcccc
     2761 tccattgaag gaacaggtct taccgatgag gagtttgccg agcagctgtt aaaggaacag
     2821 catgtcgcgg ttgtgcccgg cagcgcgttt ggtgaaagcg gtcgcggtca tgtacggaca
     2881 tgttatgcgg tggatcggtc gaaactttcc gaggctgttc gccgtatcgg aacatttgtt
     2941 gagtcgctgc acagctgaag tatttaaccc ctttgtttcg agtggaggag tttatatact
     3001 cctcccaatt ttccatgcag aagagagaga tcgtcgcacg attccttgag gcgggactgc
     3061 tggttcaccc ggcggttgtt acctatatta gtgagagcgg cggttccggt atcattgaag
     3121 gtattatcag ttcacttcct tcctccgttt cggtggttac accaaaggat gtgccgggta
     3181 tgacagcggc cagggttgac aagcttgagg ttggtgtgag tgctttaccg gagattcttg
     3241 cgggaagggc ggggtcatct gttccgacgg caacaccgga ggagtccttt gctttgttcc
     3301 gtgatcggta tgatgctctt gctgcgatga tgcggccccg tgtcagcccg atcccgatcg
     3361 aggcactggt caaacagaac aaccggtttg ccgatacgga tattggtgct atcggtatgg
     3421 ttgttgagag ttcaaccagt gcgaaaggcc atcgtattgt ggagattgag gatcctaccg
     3481 gtacgatcaa ggtactgttc aacaaaaacc gggaaggttt tgatgaagcg gaaaagatca
     3541 ttcccgatga ggtcattggc gttcgcggaa aacttgcccg cgatactggc ggcgggggcg
     3601 gcatgatctt ttctgacacc ctgtttcgtc cggaaattcc gctgacccat acgatctctc
     3661 cgtccaaaga gccaggcaaa gctgttctca tctcggatgt acatgttggg agtgatacct
     3721 tccttccgga tgcctgggac aggtttgctt cgtggctgga gagcacgcca gaggtcaagt
     3781 atctcctgat cgcaggagat gtggttgatg gtatcggtgt gtacccgaat caggacaagg
     3841 agctgcacat caaaacgatt tatgaacagt atgatgcggt aggtgagatg ctttcatccc
     3901 ttccgtcgca tctgcagatc gttctttcac ccggtaatca cgatgcggtt cgggccgcag
     3961 agccccagcc ggttcttccc gaagagttcc gaactcggtt cccgagcaat gttacgtttg
     4021 tggaaaaccc agcggttgta aacatccagt cggtgaatgt tctgatgtat cacggacgca
     4081 gtattgatga tctgatcaag tttattcccg gggcaagcta tgatcatccg ggagagatca
     4141 tggaggcgat gctgaagcgt cgccatctcg gtccgatcta tggtcagaga acaccgctgc
     4201 tttccactcc gacggatcat ctggtgatcc gggatgttcc ggacattctt cataccggtc
     4261 atgtccatat tacggatgtc atcagttatc ggggggtact tggcgtgaac tcgggaacct
     4321 ggcagggcca gacctctttc cagaaacaga tgaacattaa cccgacacca ggggaagcaa
     4381 tcgtggtgga tctgtttact atgggctacg aaaagatgtg ctttcataca taacacataa
     4441 tcttcccaga ttctttctgc gcttcctgtt cgtcaataat cgaaactctt tcgtacgggg
     4501 ttgtttcagt gaataccgga aactctttta acagtagtat tattaggtag tctgtcctgt
     4561 actatagaga gaaaccaaac cgggtatccg gaggtttttg attcgtatgg atctgatcta
     4621 tctcgctcca atatgtgccg ttatcggcct tctcttcgcc ctttactctt actctgtggt
     4681 aaagaaagag ggaaccggcg atgagaagat gcagaagatc tctgcagcca ttcaccttgg
     4741 cgcaatggtc tttctgaacc gtcagtatcg cgcaatcgga gcctttgttg ttgttattgc
     4801 aatcattctt gcaggtctct ccttcgtcac tgacgacagt atgagcattt gggcagcacc
     4861 cgcatatgtc attggtgcag ctctttcctc ccttgcagga tacattggta tgcacagcgc
     4921 aaccaaggca aacgtaagaa ccaccaatgc cgcaaaacgc ggtattgctg atgcagtcaa
     4981 agtttccttt gccagcggct cggtcatggg tatgtccgtg gtcggtctcg gtcttcttgg
     5041 tgcatctgtt gttttccttg tccttatcgg catcttcggc ggattcggtg acattgttgc
     5101 agtcaacacc gtagtctcca acatggcagc ttttggtttt ggtgcatcct ctgttgcact
     5161 cttcgcccgt gtcggcggcg gtatctttac caaagcagct gatgtcggtg ctgaccttgt
     5221 cggtaaggtc gaagcaggta ttcctgagga tgatccccgt aacccggctg tcgttgcaga
     5281 caacgtcggt gacaacgttg gtgatgtcgc aggaatgggt gcagacctgt tcgagtccta
     5341 tgttggttcg atcattgcat ccatggctct tggtatcgtt ggtgcaacct ttgcacttga
     5401 gcagtttggc gagaatgcaa gtgcactcaa tgccatcctc ctgccgatgc ttatcgctgc
     5461 attcggtatc attgctgctg caatcggaac gctctttgtt agaacaaaga agaatgagaa
     5521 ctctgcaatc caca
//
LOCUS       DALUAJ010000028         2186 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_5212,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000028 DALUAJ010000000
VERSION     DALUAJ010000028.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5212"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="O0X37_00515"
     CDS             complement(<1..201)
                     /locus_tag="O0X37_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61442.1"
                     /translation="MKEKDRTLLLAADELARTVDALAIISFLNKDTEINLNTPVVWIR
                     DIQPEILRDRTMVTLVDYCSNHI"
     gene            complement(198..1073)
                     /gene="sucD"
                     /locus_tag="O0X37_00520"
     CDS             complement(198..1073)
                     /gene="sucD"
                     /locus_tag="O0X37_00520"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HJK61443.1"
                     /translation="MIYADKDTEILVQGATGSQGAFHINLMNQYAKAVGGAGVVAGMT
                     PGKGGQQVHGVPVYNTVWDAMDHHDIGASVIFVPASGCGDAIMEAADARIPTAVVITE
                     HIPVHDVMRAIAYAKTCGTKVIGPNCPGFLIPDECKLGIIPTNLCSKGDIGVVSRSGT
                     LTYEVISELSLAGLGQSGIIGIGGDPIIGQTFSDVMDVFVEDGRTRATVLIGEVGGNL
                     EIRGAKRALSLGMPVVAYIAGIAAPKEKRMGHAGAIVEGGESDAASKIERLRELGIPV
                     ATRPSEIPLLVKEVI"
     gene            complement(1070..2158)
                     /gene="sucC"
                     /locus_tag="O0X37_00525"
     CDS             complement(1070..2158)
                     /gene="sucC"
                     /locus_tag="O0X37_00525"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HJK61444.1"
                     /translation="MKFREHEAKQIFRDAGIPVPNSELINRAGEAAAAQARVADHVVL
                     KAQVDVGGRGKAGGILPATDENISEVAKELFGKTIKGLPVEQVLVEEALDIQHEYYLS
                     ITIDRSAKMPVILFSEEGGVEIETLAKERPEALRRAFIDPFFRDVPDFILRNVLGSAP
                     KSIGPVINALYHVYCKTDAILAEINPLVMTPKGIIAADGKIVLDDNALVRQGISENRD
                     LTPREAEAEKHGFSYVELGGDIGVIGNGAGLTMATLDIIAHYNGRAANFLDVGGGAGF
                     ERVARAVRLVASMPGVKVIVVNLLGGITRCDEVAHGIIDAGVPQPVIVRLAGTNEAEG
                     RRLLEEKGYSMLDTMDQAIRAAVEVANK"
BASE COUNT          542 a          620 c          505 g          519 t
ORIGIN      
        1 gatgtgattg gaacagtaat caaccagcgt taccatcgta cgatcacgaa gaatttctgg
       61 ttgaatgtca cgaatccaga caaccggcgt gtttagattg atttcagtat ctttgtttag
      121 gaaagaaatg attgcaagag cgtccactgt tctggcaagc tcgtcggcag cgagaagcag
      181 tgtacgatct ttttccttca tatgacctcc ttcaccagga gcggaatttc tgacgggcgg
      241 gttgcaaccg gaataccgag ttctctcagc cgctcaatct tggacgcggc atcactctcg
      301 ccaccttcaa caattgcacc ggcatggccc atgcgttttt ccttgggagc agcgattccg
      361 gcaatgtagg ctacaaccgg catgccaaga gataaggcac gcttcgcacc gcggatctcc
      421 agatttccac caacttcgcc gatcagtaca gtagcgcggg ttctaccatc ctcaacgaat
      481 acgtccatca cgtcagaaaa tgtctgaccg ataatcggat ctccgccgat accgataata
      541 ccagattgac caagtcccgc aagagacagc tccgaaataa cctcatatgt cagtgttcca
      601 ctgcgcgaaa caacgccgat atcacccttg gagcagaggt ttgtagggat gatgccaagt
      661 ttgcactcgt cagggatcaa aaatcctgga cagttcgggc cgatgacctt cgttccacag
      721 gtttttgcat aggcgattgc ccgcatcaca tcatgcaccg gaatatgctc agtaataaca
      781 actgccgttg gaatccgtgc gtcagctgct tccataatgg catcgccgca cccgcttgct
      841 ggaacaaaga tgactgacgc accaatgtca tggtgatcca ttgcatccca gacagtgttg
      901 tacacaggta cgccatgtac ctgttggccg ccttttccag gagtcatgcc ggcaacaaca
      961 ccagcaccgc ctactgcttt tgcgtactgg ttcatcaggt tgatgtggaa tgcgccctgt
     1021 gaaccggtag ctccctgcac cagaatttcc gtgtccttat cagcatagat catttgtttg
     1081 ccacctccac tgctgcacga atcgcctgat ccattgtatc aagcatggaa taacccttct
     1141 cttctaggag tctacgacct tcagcctcat ttgttccggc aagccggaca atcaccggct
     1201 gcggaacacc ggcatcgatg atgccgtgag caacctcatc acagcgcgta atacctccaa
     1261 gaaggttgac tacaatcacc tttactccgg gcatggacgc aacaagacgc accgcacggg
     1321 caacacgctc aaatccagcc ccgcctccta catcgaggaa gtttgcagca cgaccgttat
     1381 agtgggcaat gatgtcgagc gttgccattg tcaggccagc tccattaccg ataacaccaa
     1441 tgtcaccacc cagctccacg tatgaaaatc catgcttctc cgcctcggcc tcacgaggag
     1501 ttaagtcgcg gttctcagag ataccctgac ggacaagggc attgtcatcc agtacgattt
     1561 taccgtccgc agcaatgatg cctttcggtg tcatcacaag cggattgatc tcagcaagga
     1621 ttgcatctgt tttacagtaa acatgataga gagcgttgat aaccggcccg atagacttcg
     1681 gggcagaacc caaaacatta cgcaggataa agtcagggac atcacggaag aagggatcga
     1741 taaatgcacg gcggagagcc tccggtcttt ccttcgcaag tgtctcaatt tccacaccgc
     1801 cttcttcgga gaaaagaatt accggcatct tggctgagcg atcaatggtg atactgaggt
     1861 agtactcatg ctgaatatcg agcgcttcct caaccagcac ctgctccaca ggcagccctt
     1921 taatggtctt gccgaacagt tcttttgcaa cttcagatat attttcatcg gtagcaggaa
     1981 gaattccacc tgcctttcca cgaccgccaa catccacctg tgctttgagg acaacatggt
     2041 ctgcaacccg agcctgagct gcggcagctt caccagcacg gttgatgagt tcactgttcg
     2101 gaaccgggat acctgcatcc cggaagatct gttttgcttc gtgttcgcgg aatttcatgt
     2161 tctctgctct ctaaccagct ccatac
//
LOCUS       DALUAJ010000029         2611 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_5231,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000029 DALUAJ010000000
VERSION     DALUAJ010000029.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5231"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="O0X37_00530"
     CDS             complement(<1..962)
                     /locus_tag="O0X37_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61445.1"
                     /translation="MRKLLLLTAVLLTVTAVILSAGCVQFHQDTDTTPTPTPEQVLPT
                     ASPTSTTTPVATATPKPAQTQMPSHKIDGSGSMETWQIWLDTGVSRVSAKNYGSGNFI
                     VWLSKGGKHQDMLFNELGAYEGETAFEVEEAGYYTFDVIASGTWTLTVNGPYDVNVSS
                     FNDYRSNGIMFVTGANTMTSSPFTLEKGVAVFNGYYLGSDRITATLYANGISAGTIDS
                     DTGAAKTQKVYPVTIAGSYTLNVNTAGEWGFEVTQPLPRNPEPFTSISGTGDEVTPYY
                     AVTGDQMLTMSNAGEGPATIVFYQPDGSIAGSVVIPAGVTGYNYL"
     gene            1057..1257
                     /locus_tag="O0X37_00535"
     CDS             1057..1257
                     /locus_tag="O0X37_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61446.1"
                     /translation="MQLFPNIETAVVTEGFLIMMTILGYFLLSGSVAETGDSTPLDVF
                     IIVMAVLVSVPPLWILRQYQGR"
     gene            complement(1309..1839)
                     /locus_tag="O0X37_00540"
     CDS             complement(1309..1839)
                     /locus_tag="O0X37_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HJK61447.1"
                     /translation="MAYIPPITAHLITLFISALGLAFLVLYRRKDSWENRQENGRPEL
                     ILSFLREHPGSSQQQIADALGMPRSTLRHYLHRMQKKQITSYQYQERPYYFAAGTSYT
                     ETERKICIILSREHEAQALTAIRQHPGITRRELAKLLHLSPSACDWYLQRFLEEDLLI
                     TIFDGIHNPLQAHPRS"
     gene            complement(2118..2564)
                     /locus_tag="O0X37_00545"
     CDS             complement(2118..2564)
                     /locus_tag="O0X37_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61448.1"
                     /translation="MKHLKKRFTAAIILIAFTGSANAAVVEQQVSFEILTDDQVMSSW
                     ASSVNGDVLQGDTDRYAYTPINGQQFEVSLTWDRAASGNYLALYIYPPDSNSIKIHDN
                     TDGKFDGKISLRTVISTDDLNQLWIFGVTGDQVSGTQSSTLTINSY"
BASE COUNT          570 a          592 c          755 g          694 t
ORIGIN      
        1 agataattat agccggtcac acctgcgggg ataacaacgc ttcctgcaat gctgccgtct
       61 ggctggtaga agactatggt tgccggccct tccccggcgt ttgacatggt gagcatctga
      121 tcaccggtga cagcataata cggcgttact tcatcgcccg ttccgctgat ggaggtgaac
      181 ggctccgggt ttctcggcag cggctgggta acctcaaagc cccactcccc ggcggtgttg
      241 acgtttaagg tgtaggatcc tgcaatcgtt accggataga ccttctgggt cttggcggct
      301 ccggtgtccg agtcgattgt tccggcagag ataccgtttg catacagggt tgcggtgatg
      361 cggtccgagc caagataata tccgttaaag accgcaaccc ccttttcgag ggtgaaagga
      421 gatgaagtca tggtgtttgc accggtcacg aacataatgc cgtttgaccg gtagtcgttg
      481 aacgaactaa cattgacatc atacggtccg ttgacggtga gggtccaggt accggaagca
      541 atgacatcaa aggtgtagta tccggcctct tccacttcaa aggccgtctc cccttcgtag
      601 gctcccagtt cgttgaagag catgtcctga tgtttgccgc ctttggagag ccagacaatg
      661 aagttgccgg atccgtagtt tttggcactc acccgggaga cgccggtgtc aagccatatc
      721 tgccaggtct ccatggaacc ggacccgtcg atcttgtggc tgggcatctg ggtctgtgcg
      781 ggttttggcg ttgcggttgc gaccggggtt gttgtacttg tcggtgatgc ggtcggcagt
      841 acctgttcag gcgtgggcgt cggtgttgta tccgtatcct gatggaactg cacacatcct
      901 gccgaaagaa tgactgcggt aactgtcaga agaaccgcgg tgaggaggag gagttttcgc
      961 ataccggtat ctttgacgcc ggatatgata atggttcgga aactgaccgg tccggcgaat
     1021 gtttaagggg tctgcggaca cactacgata gagagaatgc agctttttcc aaacatcgag
     1081 acggcagtgg ttaccgaggg ctttctgatc atgatgacaa ttctcggtta tttcctgctg
     1141 agcggatctg ttgccgagac cggcgactca acgccgctgg atgtttttat tattgtcatg
     1201 gcggtgctgg tgtccgttcc gccgctgtgg attctgcggc agtatcaggg aaggtaagga
     1261 cgcggggtcg gcagagattt cattggagat ttgcttctcc aggcatcttc agcttcgcgg
     1321 gtgagcctgt agtgggttgt gaatgccgtc aaagatagtt atgaggagat cctcctcaag
     1381 aaaccgctgg agataccagt cacaggcgga ggggctgaga tgcaggagtt tggcgagttc
     1441 gcgtctggtg atgcctggat gctgccggat ggcggtgagt gcctgtgctt catgttcccg
     1501 cgagagaatg atgcaaattt ttcgttcggt ttcagtatag gatgttcctg cggcgaagta
     1561 gtacgggcgt tcctgatact ggtaagaggt tatctgtttt ttctgcattc tgtggaggta
     1621 gtgacggagg gtgctgcggg gcatgcctag tgcgtcagcg atctgttgct gagaactgcc
     1681 gggatgttcc cggaggaaag aaagaatcag ttcaggtctg ccgttctcct gccggttttc
     1741 ccagctgtcc ttccggcggt agagtacgag gaatgcaagg ccgagagcgg agatgaagag
     1801 ggtgatgagg tgtgcggtga tggggggaat gtacgccatt ggttcagtta tgagttgccg
     1861 gagaatttct tttggtggca gttcccagag ttgaacagtt tttatttcag tcccgtcata
     1921 gatgggttca atttgttcat caactacctc aaatgcatat ttgttatgtt ccagtggttt
     1981 ggagggggca gtgggatcat gattgaatcc ataaatctcc cctccctctg agaaaaaaag
     2041 tacagctgcc gcgctgaaca gaacgagtat cgcaagtagt acaataccga tctcccgcaa
     2101 cagttctctt tccatagtta gtagctattg atagtcaagg tagatgactg tgttcctgaa
     2161 acctgatccc cggtcacacc aaatatccaa agttgattga ggtcatctgt ggatattacg
     2221 gtcctgagtg atatttttcc atcaaatttt ccatcggtgt tatcgtgtat cttaattgaa
     2281 ttgctatcag gaggataaat gtacaaagcc agatagtttc cggatgctgc ccggtcccaa
     2341 gtgagagaga cctcaaactg ttgtccgttt attggggtgt aggcatagcg atctgtatct
     2401 ccctgaagaa catcaccatt caccgaggat gcccatgaac tcataacctg atcatccgtc
     2461 aggatctcaa acgagacctg ctgttcgacg acggcagcat ttgccgagcc tgtgaaggca
     2521 atcaaaatga ttgccgcagt gaatctcttt ttgagatgct tcatgaacca gtgtcttttc
     2581 aggatccgtt ataaattcat tgaccaaagt c
//
LOCUS       DALUAJ010000030         2457 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_5517,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000030 DALUAJ010000000
VERSION     DALUAJ010000030.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5517"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /gene="tsaA"
                     /locus_tag="O0X37_00550"
     CDS             <1..436
                     /gene="tsaA"
                     /locus_tag="O0X37_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HJK61449.1"
                     /translation="IQPPAAKSFTGTIEILPELADGLTGIEGFTRLILIYQLHKSEGF
                     SLTATPFLDTTTHGVFATRVPRRPNQIGFSVVKLLGRKGNVLEIGEVDILDGTPVLDI
                     KPYVPPFDAYPQERYGWFEGKLGAIESARSDDRFTGACRNGQ"
     gene            531..740
                     /locus_tag="O0X37_00555"
     CDS             531..740
                     /locus_tag="O0X37_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HJK61450.1"
                     /translation="MKISARNQIKGKIVKLTEGAVNTEVILEIAGGQQIASIITKTSA
                     QNLGLAVGKEAYAIIKASEVMIGTE"
     gene            complement(787..1842)
                     /locus_tag="O0X37_00560"
     CDS             complement(787..1842)
                     /locus_tag="O0X37_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJK61451.1"
                     /translation="MLEISGLTLSLGEFRFTDLSVSVANGEYCIILGPTGAGKTILLE
                     TIAGIHSPDKGQIFLDGADITNAPPEQRGIGMVYQDYMLFPHMTVKENIGFGLRQRGV
                     PKGERDTAAETMAKTLGIAHLLDRYPATLSGGEQQRTAIARALVLNPRVLLLDEPLSA
                     LDTVTRDRMRREIKEIHRKTGVTILHITHHFEDIYALADRVVVMKDGEVAQEGTPETI
                     LRRPASDFIANFTGMENLFSGTAVCNEDGTAELSVGSVKLFAATDLIGQVRIGIRPED
                     LILSTKPLDSSARNSLPGTVTGIAENGIYSKISVECGDEMVLTAALTRQSVERLKLHA
                     GAEVWVTFKATAVHVFR"
     gene            complement(1835..>2457)
                     /locus_tag="O0X37_00565"
     CDS             complement(1835..>2457)
                     /locus_tag="O0X37_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJK61452.1"
                     /translation="TLCCVAVAVPVAYVMTRTPFAGKRWINILLNLPLSLPPLVAGVA
                     LLIFLSPVTSPVGQLLSGFCIFIVYTPVAIVIAQFFVNLPYMIRIARSTFAMISPRYE
                     HVARTLGCSEWRAFSQVTLPMAKSGLAAGIVITWSKAMGEFGAVLMLAGAIAMKTETL
                     PIALFLNMPTGDLDMAIAAALILLVISGVTLLIVECAGDGRGAVYA"
BASE COUNT          637 a          623 c          583 g          614 t
ORIGIN      
        1 catccagccc cctgctgcaa aatcctttac cggtaccatt gaaatcctgc cagaacttgc
       61 tgacggactg accggtattg aaggattcac tcgtctgatt ctgatctatc agctacacaa
      121 aagcgaaggt ttctctttga ctgcaacgcc cttccttgac acgacaactc acggcgtttt
      181 tgcaacccgt gttccgcgcc gtccgaatca gatcggtttc tctgtggtga aacttcttgg
      241 gcgtaaggga aatgtcttgg agatcggtga agtggatatc cttgacggaa ctcctgttct
      301 cgacatcaaa ccgtatgttc caccgtttga tgcatatccg caggaacgtt atggctggtt
      361 tgaaggaaaa cttggcgcga tagaatcggc acgctcagac gatcgtttta caggagcctg
      421 tcgcaatggc caatgatatt tactcgaaaa tttggtaaat gttgtaaaat aagttcaata
      481 aacgcagtgc gttatatgga atctctgcaa atatatatgc tgaaacaact atgaagatca
      541 gcgcaagaaa ccagatcaaa ggaaagattg tgaaactcac tgagggtgca gtcaacaccg
      601 aagttatcct tgagattgcc ggcggacagc agattgcctc catcatcacc aaaacatccg
      661 cacagaacct tggacttgca gtcggcaaag aagcctatgc aatcatcaag gcaagcgaag
      721 tcatgattgg cactgaataa agtttccccc tcattttcct ttctttattt ccatcatctg
      781 ttctagtcac cggaacacat gtactgcagt tgctttgaag gtgacccaga cctctgcacc
      841 agcatgaagt ttcaatcgtt ccacactctg tcgtgtaagt gccgcagtca acaccatctc
      901 atccccgcac tcaactgaaa tctttgagta gataccgttc tctgcaatcc ccgtcactgt
      961 cccaggaagt gagtttcgtg ccgaagagtc cagtggttta gttgagagaa tcagatcttc
     1021 cggtcggata ccaatccgta cctgcccgat aagatccgtt gcggcaaaca gtttcacaga
     1081 acccactgaa agctcagcag ttccgtcttc attgcatacg gcagttccgg aaaacaggtt
     1141 ttccattccg gtgaagtttg caataaagtc tgatgctggt ctgcgaagaa ttgtctccgg
     1201 cgttccttct tgggcaacct cgccatcctt cattaccaca acccggtcag caagtgcata
     1261 gatatcctca aagtggtggg tgatatgcag aattgtcacg ccagtttttc gatgaatctc
     1321 tttaatctcc cgtcgcatcc tatctcgtgt gacggtgtcc agagcggaaa gtggttcatc
     1381 cagcagaagt acccgtggat tgagcaccaa tgcccgtgca attgccgtcc gctgttgttc
     1441 tcctccagaa agcgtagcag gatagcgatc cagcagatgg gcgatgccaa gtgtttttgc
     1501 cattgtttcg gctgcagtat cacgctctcc ttttggaact ccgcgctgac gaaggccgaa
     1561 tccgatgttt tccttaaccg tcatgtgggg aaaaagcatg taatcctggt agaccatccc
     1621 aatacctcgc tgttcaggcg gagcattggt gatgtcagct ccgtccagaa atatctgtcc
     1681 tttgtcagga gagtggattc ctgcaattgt ctccagaaga atggtctttc ctgcgccggt
     1741 cggtccaaga atgatacagt actctccatt tgcaacggat acggagagat cagtgaaccg
     1801 gaactcacct agcgagaggg tcagtccaga tatctcaagc atagaccgca ccccgtccgt
     1861 ctccagcaca ttcaacgatc aaaagcgtta cacccgagat caccaggagg atcaacgctg
     1921 ctgcaattgc catatccaga tctccagttg gcatgttcag gaacagggca atcggcagtg
     1981 tctctgtctt cattgcaatt gcacctgcaa gcatgaggac tgcgccaaac tctcccatcg
     2041 cttttgacca ggtgatgaca atacctgctg caagtccgct ttttgccatc ggaagagtta
     2101 cttgtgaaaa cgcccgccat tcagaacagc ccagtgtccg tgcaacatgt tcataccggg
     2161 gactgatcat tgcaaaggtg gatctggcta tccggatcat ataggggagg tttacaaaga
     2221 attgtgcgat cacaattgcg accggtgtgt acacaatgaa gatacaaaaa cctgaaagga
     2281 gttgtccaac tggagaagta accggtgaga gaaagatcag cagggcaact cctgccacaa
     2341 gtggcgggag tgatagcggg aggttgagaa gaatgttgat ccatcttttt ccggcaaatg
     2401 gtgttcgcgt cattacatat gcgacaggaa ctgccaccgc tacacagcag agggtgg
//
LOCUS       DALUAJ010000031         3521 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_5764,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000031 DALUAJ010000000
VERSION     DALUAJ010000031.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5764"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="O0X37_00570"
     CDS             complement(<1..754)
                     /locus_tag="O0X37_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJK61453.1"
                     /translation="MFFHLIVTDDCNLCCSYCRAKMFEEEDPAGGTPGTIDETIAETI
                     EYPLEDLYQFLAKDPDCVLTFIGGEPLLRSDLVMEIMDHAPVRRFMLQTNGTKLGELP
                     SSYVNRFETILISIDGDQTLTDGHRGDGIYETVLKTVKLIRDHGYQGELIARMTVAED
                     TDIYAAVTHLAKHFSSIHWQMDADFTGDYSHRRFADWKETYNDGVQKLAAEWVRRIET
                     TGCVPKWYPFLSTTEDMLSGAASRLRCGSGYSN"
     gene            complement(754..1932)
                     /gene="pscS"
                     /locus_tag="O0X37_00575"
     CDS             complement(754..1932)
                     /gene="pscS"
                     /locus_tag="O0X37_00575"
                     /EC_number="2.5.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-phospho-L-seryl-tRNA:Cys-tRNA synthase"
                     /protein_id="HJK61454.1"
                     /translation="MKCAANIDARMVDEIAINLDPIQVAGRLTPEAMKAIIAWGDGYS
                     VCDNCRKPFRLDYIQNPPLKEFHVELAEWLGMDQARTVPGARRAFQQVAGTYVEKGDP
                     VLIGALAHYTSYLSVEIVHGVVREIPKTADNHITAEATAERIEDVIREFGKAPKLLYI
                     DHVDYQFGNMHDVKGIAKVAHQYDVPVLYNGVYTVGVMPVNGKDLGVDLVVGSGHKSM
                     AAPAPSGVLATTEERADEVFRTTKMTGDLTGRTFGIKEVGIIGCSLMGAPIVGLLASF
                     PRVKARVEHFDEEMANSKIVVDALKSIEGTKILSEYPRKHTLTRVDTTGSFDVVAQTH
                     KKRGFFLSSALGKKGITGIIPGATKIWKFNTYGMTRKQAEYVADTYLTIAEDNGLRVA
                     "
     gene            complement(1929..2108)
                     /locus_tag="O0X37_00580"
     CDS             complement(1929..2108)
                     /locus_tag="O0X37_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61455.1"
                     /translation="MSQRAADALFTALFSLTDIRALLRETAPTHEFDESQKTAAAKAL
                     ASVKKQIAILEEELL"
     gene            complement(2187..2546)
                     /locus_tag="O0X37_00585"
     CDS             complement(2187..2546)
                     /locus_tag="O0X37_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HJK61456.1"
                     /translation="MTASQLSETETAVLAHIRAKSDGAFQSELWKDLGVDSRTCSRIL
                     KKLEESGYIEREECKKDGTRTYLVRIVKSEKSIDPMLLMAGDVIVPCVACDEECDVEH
                     CKMLEDWVYELVFSEME"
     gene            complement(2553..2786)
                     /locus_tag="O0X37_00590"
     CDS             complement(2553..2786)
                     /locus_tag="O0X37_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoprotein"
                     /protein_id="HJK61457.1"
                     /translation="MVSSIVLPIKKVNSLVDSKISVEIKDEGRKFEGRLVAVDEYLNL
                     HLEEAIEVSETTHRNLGTVVIRGNNILSISPIN"
     gene            complement(3012..3512)
                     /locus_tag="O0X37_00595"
     CDS             complement(3012..3512)
                     /locus_tag="O0X37_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator of amino acid metabolism, contains ACT
                     domain protein"
                     /protein_id="HJK61458.1"
                     /translation="MWHSILERFSDSPSQQRVVRFLLENGFGISEEGRVVVNDTEVAA
                     TAVARVIGVDRRVVDTTVKRILGMEDIKMTFSHMRVTPDLTNVAKNLGLSVITILPKN
                     AGDKNIVASAVAVLAAHDLTLRQIFVTDPYIVESPRLVLIIDGVIPAAAIEELRALPA
                     VDSIIL"
BASE COUNT          835 a          875 c          809 g         1002 t
ORIGIN      
        1 aatttgagta tcctgaaccg cagcgcagcc tgcttgccgc acctgaaagc atgtcttcgg
       61 tagttgataa gaatggatac cactttggta cacagcctgt tgtttcaatg cgtcttaccc
      121 actctgctgc aagcttttgg actccgtcgt tgtaggtctc cttccaatct gcgaacctgc
      181 ggtgtgagta gtcacccgta aagtcggcgt ccatctgcca gtgtatcgag gaaaaatgtt
      241 ttgcgaggtg tgttaccgcc gcataaatgt ctgtgtcctc tgcaactgtc attctggcaa
      301 tcagctcacc ttgatatcca tgatcgcgga tcagttttac agttttcagc accgtctcat
      361 agattccatc cccgcgatgt ccgtccgtca gtgtctgatc tccatcaatc gagatcagaa
      421 ttgtctcaaa ccggtttacg tatgagctcg gtaactctcc gagttttgtt ccgtttgtct
      481 ggagcataaa ccggcggacc ggggcgtgat ccatgatctc catcacaaga tcagatcgca
      541 gcagcggctc gcccccaatg aatgtcagta cacagtcagg atcttttgca agaaactgat
      601 acagatcctc aagcggatac tcgatggtct ccgcgattgt ctcatcgatc gttcccggtg
      661 tacctcctgc cggatcctcc tcttcaaaca tctttgcccg gcagtaggaa cagcagagat
      721 tacagtcgtc agttacgatc agatgaaaga acattatgca acccggagac cattatcctc
      781 agcgatggtg agataggtgt cggcaacgta ctctgcctgt tttctcgtca tgccgtatgt
      841 gttaaacttc cagatctttg ttgcccccgg gatgataccg gtaatacctt tctttccgag
      901 tgcactggag aggaagaatc cgcgtttctt gtgtgtctgt gcaaccacgt caaacgaacc
      961 tgttgtgtcc acacgggtca gtgtatgctt cctcggatac tcggagagaa tcttggtacc
     1021 ttcgatagat ttcagtgcgt ccacgacaat tttgctgttt gccatctcct catcgaagtg
     1081 ttccacccgt gcttttactc ttgggaaaga tgcaagcagt cccacaattg gtgctcccat
     1141 taatgaacag ccaatgattc caacctcttt gatgccaaat gttctgcctg tcagatcacc
     1201 tgtcatcttt gttgtgcgaa acacctcgtc agcccgctcc tcggttgttg ccagaactcc
     1261 tgatggtgcg ggggctgcca tacttttgtg gcccgaacca acgacaagat caacaccaag
     1321 gtcttttcca tttaccggca ttactccaac ggtatacaca ccattgtaca gaaccgggac
     1381 atcatactgg tgtgcgactt ttgcaattcc tttgacatcg tgcatgttgc cgaactggta
     1441 gtccacgtga tcaatgtaga gcagttttgg tgcttttcca aactcacgga tcacatcctc
     1501 aattctttct gccgtagcct ctgcggtgat gtgattgtca gcggtctttg gaatttcccg
     1561 gacgactcca tgtacaattt ctacagaaag atacgacgta tagtgggcaa gggcgccgat
     1621 cagtactgga tctcctttct ccacatatgt tcctgcaacc tgctggaatg ccctgcgtgc
     1681 acctgggaca gtacgtgcct gatccattcc cagccattcg gcaagctcca catgaaactc
     1741 ctttaaggga ggattctgga tataatccag acggaacggt tttctgcagt tgtcacagac
     1801 tgagtaaccg tcaccccacg caattatcgc cttcattgct tccggtgtca ggcggccggc
     1861 aacctgaatc ggatccagat tgatggcaat ctcatcaacc atgcgtgcat caatgttcgc
     1921 cgcgcacttc ataacaactc ctcctcaaga atggcaatct gttttttgac ggatgcaagg
     1981 gcctttgctg cggctgtttt ctgagattcg tcgaactcat gggttggcgc agtttcccga
     2041 agaagtgcgc gaatatctgt taacgaaaac agggcggtaa aaagagcatc tgcggctcgc
     2101 tgtgacatga tgcataaaac atctacgcct gcataaaata acgtatcgat tctctgaaaa
     2161 atagggtggg tatttttccg gtttatttat tccatttccg aaaagacgag ctcataaacc
     2221 cagtcttcga gcattttaca gtgttcgacg tcacactcct cgtcacaggc aacgcatgga
     2281 acaataacat cacctgccat cagaagcata ggatcaatgg acttttcgga ttttacaatc
     2341 cgcacaagat atgtacgggt tccgtctttt ttacactctt cacgctcaat gtatccgctc
     2401 tcctcaagtt ttttgaggat gcgtgagcag gtcctgctgt caacacccag atctttccac
     2461 agttcgctct ggaatgcacc gtcggatttc gctcggatgt gcgcaagtac agctgtctct
     2521 gtttcactca gttgggatgc ggtcatggga agttagttga tgggggagat actcaggatg
     2581 ttgtttccac ggataaccac agtaccgagg ttccggtggg ttgtttcact tacctcaatt
     2641 gcctcttcaa gatgaagatt gaggtactca tccactgcaa caaggcgtcc ctcgaatttg
     2701 cgtccctcat ctttaatctc tacggaaatt ttggagtcta cgagggagtt gacctttttt
     2761 attggaagga caatactgga taccatatcg ttatttttcg ttacccagac taattaacat
     2821 gattgtttga tgggggatgt ctggggtgtc tgcacatctt cagagtgtat cagtaaatct
     2881 actctggaat gtgtattatg aatgtaaaac gaatctggga tctgtgtaaa atgaggaata
     2941 ttatttttca gttggttttt tttccctctt ttggatctca gattttccag atatcagtca
     3001 cactgggaat attacaggat aatggagtca accgccggta gggcgcgcag ttcttcaatt
     3061 gctgctgcgg gtatcacacc gtcaataatg agtacgaggc gcggggactc tacgatgtag
     3121 ggatcggtta caaaaatctg gcgaagagtg agatcatgtg cagcgagaac tgcaactgca
     3181 gatgcaacga tatttttgtc accggcattt tttgggagga tggtaattac ggaaagcccg
     3241 agattttttg caacatttgt gagatctggt gttacccgca tatgggaaaa cgtcattttg
     3301 atgtcttcca ttccaaggat acgtttgacc gttgtatcca caacacgccg gtcaactccg
     3361 atcactcggg ctactgctgt cgctgcgacc tcggtatcat ttactactac tctcccttct
     3421 tcactgatgc caaatccgtt ctcaagaaga aatctgacaa cccgctgctg ggatggtgag
     3481 tctgaaaatc gttcaagaat gctatgccac atgtacaaaa a
//
LOCUS       DALUAJ010000032         4415 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_5987,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000032 DALUAJ010000000
VERSION     DALUAJ010000032.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5987"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="O0X37_00600"
     CDS             complement(<1..637)
                     /locus_tag="O0X37_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HJK61459.1"
                     /translation="MSSLGIIHHCDCITGMNRLSAGSVDVIVTSPPYNLGKLYTTYDD
                     TVPRNGYLSWMKDVADAAIRVLSDEGSLYLNVGGSLKDPWIPMDVAQEFRRSGFILQN
                     MIHWIKSIALPKEDMGKFYNAKIPENGVAVGHYKPINSHRFHNDCHEFIFHFTKTGDV
                     PIDRLANGVPYQDKSNVRRWVQKKQDIRDRGNTWFIPYETVREERPHPATFP"
     gene            complement(640..1065)
                     /locus_tag="O0X37_00605"
     CDS             complement(640..1065)
                     /locus_tag="O0X37_00605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HJK61460.1"
                     /translation="MSGVTAESTLLLHFTSRRPDGEIFEDTRSGKPVLVTLGKKQINP
                     AFEEALIGKSEGETVTVVLPPEKAYGRYHKKLVVTIKRHKLSLDHEPVAGEIIRVEVL
                     KKPCLVTVVGVTDKSITVDANHPLAGETITYDITIEKIF"
     gene            complement(1126..2076)
                     /locus_tag="O0X37_00610"
     CDS             complement(1126..2076)
                     /locus_tag="O0X37_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HJK61461.1"
                     /translation="MEIAYAVLVIVGLVAFETVASIDNAIINADILSTMKAWARRWFL
                     TWGIIIAVFGIRGVLPWLIIWFSTPSLGPIGALTATFSDDATAAAAIEASAPFLLLAG
                     GIFMIFLFLHWLMAEEKNCIVPGERAMSRQGPWFYAVAAVVLFLICYAAAQINALLAV
                     AAVGGSAIFFIMQGFRLFADKKSAELEAGTSTKSDMSKLMLLEVIDATFSIDGVIGAF
                     AFTMSVPLILIGNGIGAVVVRELTVRNIENIRRYAYLKNGAMYSIFTLGLIMCGEGFG
                     LHIPIWFAPLLTMGIVATFFWLSLRRIKKGDFGVCHIPRE"
     gene            2430..3032
                     /locus_tag="O0X37_00615"
     CDS             2430..3032
                     /locus_tag="O0X37_00615"
                     /inference="COORDINATES: protein motif:HMM:NF021414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 domain-containing protein"
                     /protein_id="HJK61462.1"
                     /translation="MNVLSLSDDESAAVIDRTIQSLQKATSTADAVKRIGSALSQYTV
                     FDLQYIGGFIRREVNKLPNPYRRAYLPYSLDLLNQYHVFMQMYRTQKIYDEPIKDPTL
                     WSEYWEIARVQCFVRSGPSDDPFPQMEHPLSKFFYRLVYGYVMLIANGYGHPIGMPFP
                     GGATVHREGEQVLCPIRDKERDLPAALCNFCPAEQDPRYK"
     gene            3113..3991
                     /locus_tag="O0X37_00620"
     CDS             3113..3991
                     /locus_tag="O0X37_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJK61463.1"
                     /translation="MYELIHARGNTYYIDCPAKMGLYVKDDAHAYLIDSGNDKEAGKK
                     ILKILQAHGWNLDAVIVTHSHADHFGGCAVLQERTGCKVYGSEIERAFMQHPILEPAY
                     LYGGYPPKELRNKFLMAKPCTVLDICEETFPHEFTIIPLPGHYFDMMGVVNPDKVFFC
                     SDSLSGEHILEKYHVAVLYDVEAYLTTLETVETTDAELFVPAHAEPVTDIRELARMNA
                     MKVEEIADQILELCGAGASFEDILAGLFVRYNLSMDCNQYVLVGSTVRSYLSYLHGKG
                     LIDIMFVDNRMLWARK"
     gene            complement(4124..>4415)
                     /locus_tag="O0X37_00625"
     CDS             complement(4124..>4415)
                     /locus_tag="O0X37_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HJK61464.1"
                     /translation="LLRSDLIGAVAAVQLARKVFSRIRMNLFWAFAYNLFLIPLAAGV
                     LYPFTHVMFRPEYAAAAMVVSSVTVVSLSLLLRRYTPPALRNNNVSPIQNTS"
BASE COUNT         1279 a          994 c         1094 g         1048 t
ORIGIN      
        1 ccggaaaggt tgccggatgg ggacgctcct cgcggacggt ttcatagggg atgaaccacg
       61 tgttgccgcg atcgcggata tcctgttttt tttgtaccca tctgcggaca ttgcttttgt
      121 cctgataggg aacaccgttt gcgagccgat cgatcggaac gtcaccggtt ttggtaaagt
      181 gaaagatgaa ctcgtggcag tcattatgga accgatgact gttgatcggc ttgtaatgac
      241 caaccgcgac accattctcg ggaatctttg cattgtagaa ctttcccatg tcctctttag
      301 gaagagcaat ggatttgatc caatggatca tattctgaag aatgaacccg gatctccgga
      361 actcctgcgc aacgtccatg ggaatccacg gatctttgag actacctcca acattgagat
      421 acagagagcc ctcatcagag agtacacgta tcgcagcatc ggcaacatct ttcatccaag
      481 agagataccc gttacgcgga acggtgtcat cataggtggt gtaaagcttg ccaaggttgt
      541 aaggtggaga ggtcacgata acatcaacag atccggcaga aagccggttc ataccggtga
      601 tacagtcaca gtgatgaata attcccagag agctcatggt cagaagatct tttctatggt
      661 gatatcgtag gtgatcgttt ctccggcaag cggatgattc gcgtccaccg tgatgctttt
      721 atcggttaca ccaaccaccg tgacaagaca aggctttttc aggacttcga ctcggatgat
      781 ctctcctgca accggttcgt gatcaagact gagtttatgc cgtttaatgg tgacaacgag
      841 tttcttgtgg tacctgccgt aagctttctc cggaggaagc acaacggtga ctgtctcacc
      901 ctcggacttg ccaataagag cctcttcaaa tgctggattg atctgttttt ttccgagcgt
      961 gaccagtacc ggtttaccag agcgagtatc ctcaaagatt tccccatctg gacgacggct
     1021 ggtgaaatga agaagaagcg tactttccgc agtgacacca ctcatgatta ttctgtatgt
     1081 tttgaatgta cattagtatt tgtccggaca aaaaggaggg attggttact cccgcggaat
     1141 gtggcaaaca ccgaagtccc ccttcttgat tctccgaagc gagagccaga agaacgtcgc
     1201 cacaattccc atggtcagga gcggggcaaa ccaaattggg atgtgcaagc cgaacccttc
     1261 accgcacatg ataaggccaa gtgtaaagat agaatacatc gcaccatttt tcagataggc
     1321 atatcttctg atattttcga tgttccggac ggttaactca cgcacaacaa ctgcgccgat
     1381 accatttcca atcagaatca gcgggacaga catggtaaat gcaaaggcgc cgataacacc
     1441 atcaatagaa aatgttgcgt cgattacttc aagcagcatc aatttactca tgtccgattt
     1501 tgttgaagta ccggcttcaa gctccgcaga ctttttgtcg gcaaagagcc tgaacccctg
     1561 catgatgaaa aagattgcag aacctcccac tgctgccacc gcaagaagag cattgatctg
     1621 tgctgcggca tagcagatca gaaacagaac aacagctgca acagcataga accagggacc
     1681 ctgacgggac atggcacgct ctcctggaac aatacagttc ttctcctcgg ccatcaacca
     1741 gtggaggaaa aggaagatca tgaagatccc tcccgccaaa aggaggaacg gtgcactggc
     1801 ctcaattgca gccgcagcag tagcatcgtc ggagaacgtt gcagtcagtg ctccaatcgg
     1861 accaagggaa ggggttgaaa accagatgat aagccatgga agcacaccac ggataccaaa
     1921 aactgcaatg ataatacccc aggtcaggaa ccagcggcgt gcccacgctt tcatggtgga
     1981 caaaatgtct gcattgatga ttgcattgtc gatactagcc actgtttcaa atgcgacgag
     2041 gccgacaatc acgagaacag cgtaggcaat ctccatgcta actaactgag gacatatagg
     2101 ggctgataga ttattaatat cgccatttgg cctgaacgtt tgtacagaaa acagaagtga
     2161 tgtatcttat ctgatagccc gaatgtgctg atgaatatat actatagaaa cggattgaga
     2221 taatggtatg aaaaagtatc tccagacggt tctgggttgt ctgtgcctaa tttcagcagg
     2281 cagtgtgaca acagtgtcag catgtgcaga gaaagaaatg gaactgacaa cccattcaga
     2341 accagccgga gcaacaatga ctgtttccga gaagcaaggg gaaatggaat ggcagtctcc
     2401 cagaacggga ctggaggaat gattcggaga tgaacgtcct ttccctttcc gatgatgaat
     2461 cggcggcagt tattgacagg accatccagt cccttcagaa ggcaacgagt actgctgatg
     2521 cggtgaaaag aatcgggtcc gctttatccc agtacacggt ctttgatctt cagtacatag
     2581 ggggatttat ccgccgcgaa gtaaacaagc tgccaaatcc ataccggaga gcatacctac
     2641 cttacagttt ggatctgttg aatcaatatc acgtgtttat gcagatgtac cgaacgcaga
     2701 agatttacga tgaaccaata aaggatccta cgctctggag tgagtactgg gaaattgcac
     2761 gcgtacagtg ttttgttcga tccgggccat cggatgatcc gtttccacag atggaacatc
     2821 ccctgagcaa atttttttac cggctcgtat acggatatgt gatgctgatt gcaaatggat
     2881 acggacaccc aatcggtatg ccgtttccgg ggggggcaac agtccatcgc gagggagaac
     2941 aggtactttg tccaatcaga gacaaagaga gggatcttcc cgcagcatta tgtaattttt
     3001 gtccggcaga acaggatccg cgatacaaat aattatttca agtaaagaga aaagcaggct
     3061 taggaatgta cggatatata acaggtacac accatacaat gatcgaattc cgatgtatga
     3121 actgatacac gcacgcggga atacgtatta tattgactgc ccggcgaaga tggggctata
     3181 tgtgaaagat gatgcccatg cctatctgat cgatagtggt aacgacaagg aagcgggaaa
     3241 gaagattttg aaaatcctac aggcacatgg atggaacctg gatgcagtaa ttgttaccca
     3301 ctcacatgca gatcattttg gcggatgtgc agttttgcag gagcggacag ggtgcaaagt
     3361 gtacgggagc gagatagagc gggcatttat gcaacatcct attctggaac cggcatattt
     3421 gtacggggga tatcccccaa aggaactcag aaataaattc ctgatggcaa aaccgtgtac
     3481 ggtactggat atatgcgaag aaaccttccc acatgagttc acgataatcc ccctgccggg
     3541 acattatttt gatatgatgg gagttgtgaa cccggacaag gtgtttttct gttcagacag
     3601 tttatcaggg gagcacatac tagagaagta ccatgtcgct gttctctatg atgtagaggc
     3661 atatcttaca acactggaaa cggtggaaac tacagatgcc gaattatttg tccctgcaca
     3721 tgccgaaccg gtaacagata tcagggaact cgcacgaatg aacgccatga aggtagagga
     3781 aattgcagat cagatacttg agttgtgcgg agcaggtgca tcatttgagg atatcttagc
     3841 gggattattt gtacggtaca atctctcgat ggactgcaac cagtatgtac tggtcgggag
     3901 tacagtacgg tcgtacctct cttatctgca tggaaaagga ctcattgaca taatgtttgt
     3961 agacaaccgg atgttgtggg caagaaagta aggagcgtat agaaaaccag gaaaaagaaa
     4021 tcctgaagaa atatttcatt gctacagtaa attccggatc ttcgtggcta ttcaaaactc
     4081 actaaagacc caaaatagag agaatggtta ctgaacaaca ctctcacgat gtattctgaa
     4141 tcggagaaac attgttgttt cgcaaagcag gcggtgtata cctccgtaga agcagtgaga
     4201 gacttaccac cgttaccgaa ctcaccacca tcgctgccgc tgcgtattct ggtcggaaca
     4261 tcacatgtgt aaacggatac aatacacctg ccgcaagtgg gatcagaaac agattgtatg
     4321 cgaatgccca gaacagattc attcggattc tcgaaaatac cttgcgtgca agctggactg
     4381 ccgcaactgc accaatcaga tccgaacgaa gtaaa
//
LOCUS       DALUAJ010000033         2251 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_6080,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000033 DALUAJ010000000
VERSION     DALUAJ010000033.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6080"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..149
                     /locus_tag="O0X37_00630"
     CDS             <1..149
                     /locus_tag="O0X37_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK61465.1"
                     /translation="CTGCGTCVDICPAASIELANDKAVVDASACVDCETCVDECPESA
                     IHME"
     gene            244..753
                     /locus_tag="O0X37_00635"
     CDS             244..753
                     /locus_tag="O0X37_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61466.1"
                     /translation="MLPQRNEYSNESEYLYHKSLILENPSEMDRVLYFYLIDAVAHLD
                     YTLSSLAYNPDSIRCVMTAEYLRHRVDQAKEGDNALFPGFMVWLRDTNQEMFENCPML
                     WQLVYDPDEPTRYVGFRIVLDPASRSPLPPQFFHDMADDLFSQQMLRSIYPTGALGKL
                     FAEYKAKHA"
     gene            762..1952
                     /locus_tag="O0X37_00640"
     CDS             762..1952
                     /locus_tag="O0X37_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HJK61467.1"
                     /translation="MDVVRICSELVKIKSENPPGDTTEVAQYIASLLDGLGIRSDITE
                     GAPGHCNVVSREQNRALMLSGHMDVVPAMEEGWDIPPYAGVIDDTYVHGRGSTDMKGG
                     CAAILTAVERAVNECGESPVSLAFVCDEEGGGRYGTRYLIEKRLIHPCDVLIAEPTPA
                     FAPSVGQKGICRFEVEFTGTPGHSSLFPIVGERAIMQAFSFLSWMDTLHKRVYPQSPA
                     LEKLIEHSIAISDMQEGQAGHELSPIFRQIMYNPGMISGGERVNIVAQKCRLTMDLRL
                     PWGCDCDEVLGEVYGHLPPSARVTPITKANASLTDPESFLVQSTAKAISDVYTIVSKP
                     MVQWAASDARALRKAGFRAIEYGPGELCGLHGLNERVRIDQLRSVEEIYYRLITMYRE
                     RFGF"
     gene            2035..>2251
                     /locus_tag="O0X37_00645"
     CDS             2035..>2251
                     /locus_tag="O0X37_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61468.1"
                     /translation="MKRIGTLLLVLLCIGAVLFTAGCVNTNSQPDPAGTTWVLAGFGT
                     DSDGAAAPAATISLTFRDSGNASGCGGV"
BASE COUNT          462 a          554 c          647 g          588 t
ORIGIN      
        1 agtgtaccgg atgtggaacc tgtgtggaca tctgcccggc agcatccatc gaacttgcaa
       61 acgacaaggc agttgtggac gcttccgcat gtgttgactg tgagacctgt gtggatgagt
      121 gcccggaaag cgccatccac atggaataaa cattcctcac cgttttcttt tttgtctgtt
      181 cttttctctg gtgtatttga cctgcatcct aacgcttttt gtctatcccg acaaactact
      241 actatgttac cgcagagaaa cgagtactcc aacgagagtg agtatctcta ccataagtcg
      301 ctgattctgg aaaatccgtc cgagatggat cgcgtcctct acttttacct catcgatgcc
      361 gttgcgcatc ttgactatac tctgagttct cttgcctaca atcctgattc tatccgttgt
      421 gtgatgacag ccgagtatct tcgccaccgg gttgatcagg caaaggaggg ggataatgcc
      481 ctgttccccg gatttatggt ctggctgcgg gatacgaatc aggagatgtt tgagaactgt
      541 ccgatgctct ggcagctggt ctatgatcct gacgagccta cacggtatgt tggattccgg
      601 attgttcttg acccagcgtc ccggtctccg ctcccgccgc agttcttcca tgacatggct
      661 gacgatctct tctcccagca gatgctcagg agtatttacc cgaccggggc tcttgggaag
      721 ctgtttgccg agtacaaggc aaagcatgcc tgaggtaagc gatggatgtt gtacgtatct
      781 gttcggaact ggtgaagatc aagagtgaaa atcctccggg cgacacgaca gaggttgccc
      841 agtatattgc atcgcttctt gacggacttg ggattcgttc ggatattacg gaaggtgcgc
      901 caggtcactg caatgttgtt tcccgggaac aaaaccgtgc actgatgctt tccggccata
      961 tggacgtggt tcctgctatg gaggagggct gggatattcc tccgtatgca ggagttattg
     1021 acgatactta cgtgcacggc cgtggaagta ctgatatgaa gggcggctgt gcggcgatcc
     1081 tcactgcggt ggagcgggcg gtaaatgaat gcggcgagag tccggtatcc cttgcatttg
     1141 tctgtgatga ggagggcggc ggccgctacg gtacgcggta tctgattgaa aaaaggctga
     1201 ttcatccgtg tgatgtgctg attgcagagc cgactcctgc gtttgccccg tcggtcggcc
     1261 agaaaggtat ctgccggttt gaggtggagt ttacaggaac tccgggccat tcctcactgt
     1321 tcccgattgt tggagaaagg gcgattatgc aggcgttttc gtttctttcc tggatggata
     1381 cactgcacaa acgcgtgtat ccgcagtctc ctgcgctgga aaaactcatt gagcattcga
     1441 ttgctatcag cgacatgcag gaggggcagg caggtcatga actttcaccg attttccggc
     1501 agatcatgta caacccgggt atgatctccg ggggagagcg cgtgaatatt gttgcgcaga
     1561 agtgccgcct gacgatggat ctccggctgc cgtggggttg tgactgtgac gaggttcttg
     1621 gcgaagtgta cgggcatctg ccgccgtccg cccgggttac ccctatcaca aaagcaaatg
     1681 cctcccttac ggatccggag tcgtttctgg tgcagagcac tgcaaaggcg atcagcgacg
     1741 tgtatactat tgtctcaaaa ccgatggtgc agtgggcagc atcggacgca cgtgcgctgc
     1801 gcaaagcagg gttccgggca attgagtacg ggccgggtga gctgtgcggc ctgcacgggc
     1861 tgaatgagcg ggtccggatt gatcagcttc ggtcggttga ggagatctat taccggctga
     1921 tcactatgta tcgtgagcgt tttgggttct gattgctccc ggcctctttt tcatttttca
     1981 aattttgtaa ccatctcgac gtttataact accacctgca aatcaattac gtgtatgaaa
     2041 cgtattggca cattacttct tgtgctcctg tgtattggag cagttctttt tacggcgggc
     2101 tgcgtgaaca caaacagcca gccggatccg gcgggaacaa catgggttct cgcagggttt
     2161 ggtaccgact ccgatggtgc ggcagcgcct gcggcaacta tctctcttac cttccgcgac
     2221 agcggtaatg cgtccggatg cggaggcgtg a
//
LOCUS       DALUAJ010000034         2436 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_6584,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000034 DALUAJ010000000
VERSION     DALUAJ010000034.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6584"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(151..1644)
                     /locus_tag="O0X37_00650"
     CDS             complement(151..1644)
                     /locus_tag="O0X37_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HJK61469.1"
                     /translation="MTQDIQKTLETFFHHQTFRPNQEEIIQDVVAGRDVLAVMATGGG
                     KSLCYQLPAMMLDGMTVVISPLIALMKDQVDALSVQGVRVETLNSTQTYDERLRVEKE
                     ISAGIVRILYVSPERAVTPAFAAILSRCRVALFAVDEAHCISMWGHQFRPEYREIKTL
                     REKFPAVPVIALTATATERVREDIVRELGMKNPREYVGSFNRKNLRYEVYEEPTSSIR
                     VQRIVSYVVAHPNVSGIIYCFSRKSCEEIAERLRRSRILASPYHAGMPTPERSRIQEG
                     FLNNSIRVICATIAFGMGIDKPDVGYVIHAHLPKDLESYYQETGRAGRAGGRADCILY
                     YSSGDRAKISSMLEREYSQSEKLRIAKQKLADMYSYCVTPGCRRKVLLSYFGEEISDC
                     GNCDRCDREKVKQKKTAVPSGDLSRIVYDAASSLSGQMTVSSFVSFLLGLDRAHTKSS
                     GLTSHPFYGAAQNHERSEVMGVVQDLIAAKKLCLKGNAVMKLCRAGK"
     gene            1741..2115
                     /locus_tag="O0X37_00655"
     CDS             1741..2115
                     /locus_tag="O0X37_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HJK61470.1"
                     /translation="MKFSMIHNNINVFDLDRSMKFYAEALGLFEVGRIAPETGDFIIV
                     YLGDGTSGHKLELTWLRDRQEPYDLGDNEFHLAFGTDDYDGALAKHTEMGCVAFINED
                     MGIYFITDPDGYWLEILPPGRG"
     gene            complement(2221..>2436)
                     /locus_tag="O0X37_00660"
     CDS             complement(2221..>2436)
                     /locus_tag="O0X37_00660"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616795.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HJK61471.1"
                     /translation="GLTRGPRVIGPETRAAMQEILEEIRSGAFAREWLLENQVNRPHY
                     TSLVRLEKESELEKVGSEVRHIISAKK"
BASE COUNT          624 a          618 c          615 g          579 t
ORIGIN      
        1 ctatgggtat cagtgcagga agtatgcggc cgctgttatg cgaaacagaa acacacgggt
       61 agcattaaag acaatatccc ctcatttttc caaacgaaaa tctccgggtg atccagtctc
      121 gaggttggaa aagtgttcgg ttatgaaaaa ttacttcccc gcacggcaaa gtttcataac
      181 cgcattcccc ttgagacaaa gtttttttgc ggcaatcaga tcctgcacaa ctcccatgac
      241 ctcggaccgt tcatgatttt gtgcagcacc gtaaaacgga tgcgaggtca gaccggaact
      301 tttggtgtgg gcacgatcca gcccgagaag aaatgaaaca aacgatgaaa ccgtcatctg
      361 cccagaaagg ctgcttgctg catcgtaaac gatgcgggag agatccccgg aagggacagc
      421 tgtctttttt tgttttactt tctcccgatc acagcggtca cagttgccgc agtcggagat
      481 ctcctcaccg aagtaggaga gtaaaacttt cctccggcat ccaggagtta cacagtacga
      541 gtacatgtca gcgagtttct gttttgcaat gcgcagcttc tccgactggg agtactcacg
      601 ctccagcata gaggatattt ttgcccgatc accggaagag tagtagagaa tgcagtcggc
      661 acgaccaccg gcacggcctg cacgaccagt ctcctgataa taggattcaa gatccttggg
      721 gagatgagca tgaatgacat agccaacatc gggtttgtca attcccattc cgaacgctat
      781 cgttgcacag ataacgcgga tgctgttgtt gagaaatcct tcctgaatgc ggctgcgttc
      841 aggggtcggc attcccgcat gatacgggct tgccagaatg cgggaacgcc tgaggcgttc
      901 tgcaatctcc tcacagcttt ttcgggagaa acagtagata atacctgaaa cattcggatg
      961 ggcaacaacg tagctgacaa tcctttgaac acggatcgag ctggtcggct cctcatacac
     1021 ctcataccgg agatttttcc ggttgaaact gccgacatac tcgcgaggat tcttcatacc
     1081 aagttcccgg acgatgtctt cgcggacacg ctcggtcgcg gtcgcggtca gggcaataac
     1141 cggtactgcc ggaaattttt cccggagtgt tttgatctca cggtactcgg gacggaactg
     1201 atgtccccac atggagatac aatgagcctc atccacggca aaaagtgcaa cacgacaacg
     1261 ggaaaggatt gcagcgaacg caggggttac cgcacgttcg ggcgatacgt aaaggatacg
     1321 aacgatccct gcagaaatct ccttttcaac tcggagcctt tcatcgtagg tctgggtact
     1381 gttgagtgtc tccacgcgga ctccctgcac tgagagagca tccacctgat ccttcatgag
     1441 tgcaatcagg ggggagatta cgaccgtcat cccgtccagc atcatcgccg gcaactggta
     1501 acaaagggat ttcccgccgc cggttgccat gacagccagt acatcacgcc cggcgaccac
     1561 atcctggatg atctcctcct gattgggccg gaatgtctga tggtggaaga acgtttccag
     1621 agttttttgg atgtcctgcg tcatatggct gaattctggt taagttatcc ggcagagttc
     1681 atattaaact agtgggattg ggaacaagag tagatatgat aaccactcca ataatcctcc
     1741 atgaaatttt ccatgatcca caacaacatc aacgtctttg atctggatcg gtcaatgaag
     1801 ttctatgctg aagcactcgg ccttttcgag gtgggacgca ttgccccgga gacgggagac
     1861 tttattatag tgtatcttgg agacggaacg tccggccaca aactggaact gacctggctg
     1921 cgggatcgcc aagagccgta tgatctcggc gacaacgagt tccatctggc gtttggtaca
     1981 gatgactatg acggagctct tgcaaaacat acagagatgg gctgtgttgc attcatcaat
     2041 gaggatatgg gcatctactt catcaccgat ccggatggct actggctgga gattcttccg
     2101 cccggacggg gataaaaatt gcttcccctt ttgaaaacag aagcacggac acgatcaact
     2161 gtcagaacag taacaaattt tctgtgatat tgagcggcga aaaagataca accataaaaa
     2221 ttattttttg gcagagataa tatgacggac ttcactgcca actttttcca gttccgattc
     2281 tttctcaagc cgcacaagtg atgtgtagtg cggacggttt acctgatttt ccaggagcca
     2341 ttcacgggca aatgctcccg aacggatctc ctcaagaatt tcctgcatcg cagcacgggt
     2401 ctccggaccg atcacccgcg gaccgcgggt cagacc
//
LOCUS       DALUAJ010000035        15029 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_6639,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000035 DALUAJ010000000
VERSION     DALUAJ010000035.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 15029)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15029)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15029
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6639"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="O0X37_00665"
     CDS             complement(<1..418)
                     /locus_tag="O0X37_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61472.1"
                     /translation="MAEGRMAYGWIAMIVIIFVVSVIYILFDPAIKTLYEAGIQLNTD
                     LIPVMDFLNLLWTWFPLAFIIGILVWGINMGIGNSPRPDRIVLAWIIIIVCILAIIAA
                     YVSLDTMVSSLISWGYQISDAFGAALSTIKIMWYNYP"
     gene            complement(418..4668)
                     /locus_tag="O0X37_00670"
     CDS             complement(418..4668)
                     /locus_tag="O0X37_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61473.1"
                     /translation="MAGAPVIQPSGGAGGASLAPDAGNAGAGATINPTEARVQQQAAA
                     AVAAQQASAAYAKRERDVREYTDALVEYHDLPDEFRSTEAPEMTMVNRRGTEISSLRS
                     PRGADLAANARDAETQRFADWERDQLFEGDKLHRARDYLYYQRERANAGVTSYFSGER
                     TGNYERDLVDVQQAYNAARNEGRDFVWEGKIADTEDLAHAFSLGMINLGANESRKEAM
                     YKAYVQDPLKIGRAGVLADPNSAESKELLEKEYQEYLKFRDTPLTFENKVYMPRVAPE
                     RIAADAAQNAARVEYNAAQSEYVANLHNFMVNTGYKSFEDLGRDIAEFKIHQASQPTF
                     TDQVRAAERADWTAYQNRSAAEQTNPTLRDYAKAIEGAANQNTYLFMSPGQNGSVDLN
                     APMYLSSVNGYRQLTPAEQKDVVGSIRISLPDPVTGRDIEFRVSGTANKGDKLESLQY
                     NTYIPLLGEPVREEVYRTDTLQFPVSVPGYEQPLWLSPTEPISEKQAESILENLGIAH
                     RDPVSREIVQNPEISGLDWSKGIASAATDLGNAIRDSVSKPLESAFTGAANLYDAWAD
                     KYIDLNRNGILKNLPGADLRIDLASGSKANTRAFFDFESGLSAAPENVVRSAGSAATL
                     AETVVKNPDLITPGNVVYAGTSAFEAMAKEAEKNPARFLGELAGETLITLPLGTAAGS
                     ALKAAPGWIGRRIGLYDVGIESPKGPAAGAISDLGALSEVIEGGPTGSYIRRVPAAKS
                     PSEGFFVRDFTLEAKNPETYPKNPEPGKREILFATPDHGYAPSWTGLGKLDGSNKVRV
                     FRLQADKVPVSPDEAMAVYREVANSPVYGKFYDTLEERGAAIAAAENKPVVVASYNKQ
                     FGKKLDEKEAEVAIIPAGGASTQKVRAYFAGFSRDGRPVWNMTNAQTNTAIAKEFLSA
                     WGERILKNNQFYVDWKAPFRRSGDRIFVPKTLLDYYGEQAALKQIQQETRILPTTYDF
                     SSHGPAHVSSVARTALANIEKYPKIYGKNIDKTALEYAAYAHDLGKIVDTDYPQTIPH
                     GQAKALSLLKGADDPLKALEKRPALKQVLLQDYPDAYKRLVADRKQWAAFTPKQQKQI
                     ADAIARHTGLDKSDPLSYLISRPSGMAKALADADKLDFTRFETAVKMEKLFAGKAMQA
                     AESAAVQKRINRVLGTTAGINIAIPINRINTDIIENIRKPAKTRRTSLDENRYPRDLT
                     PEDKYGSGYKSLAPDKGYYPAILPGNYDNYPVPAKKTYPYPAQLRETSYYPAQYPTDT
                     EYAAAYTSQYSPGTTTPAIYPGGYTPDHRVNPYPTAPAPRGGYPVAVPRTIPKTTPII
                     PRRKKSAATRKRKTAYRKIRVENIEHLSITDPLEALGIGSMTNRTRRKPQPQTTYYEY
                     DGIFSTTPPTRRKSGDILPAIEPQRKPGKRTKPKRRKR"
     gene            complement(4670..5410)
                     /locus_tag="O0X37_00675"
     CDS             complement(4670..5410)
                     /locus_tag="O0X37_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61474.1"
                     /translation="MIVLDDTTFYATLAMVAMISMCVGLFPALLAKDLVIPYLKASFG
                     KSKYCILEISKTNAIRPIPAEFHNGMAIIRDRIGRFVKLGLNGSYNLGNCRCDIVLNQ
                     VGIMSETEFVAMTDELDRLGIGSLQDMVLILNAISATSAKIPISNQSYQFASEFLRAH
                     PEYDFTVFYPLCGKMNVKKLVDWAQGSPEIISQAISEGIAEASEQYVQAATGKSSAAD
                     LKGMLPLIVIGIVGILIAVMAMKGMGVM"
     gene            complement(5407..5919)
                     /locus_tag="O0X37_00680"
     CDS             complement(5407..5919)
                     /locus_tag="O0X37_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61475.1"
                     /translation="MTDPLTTDDITRLRDLIKEAQADRDAPYVGATPPPPDPMASEVL
                     MRLISGQDEIPADLKQDFPALLAIFQQYMPVFSKKDMAIWKIRARQSLTLYKLCNPHS
                     GMTMYREAVISYLNLILLAKAQDGYERNCSISTFTTITHQQQQIRMPSGDEKQSGWID
                     RMKNAFGGHR"
     gene            complement(5894..6424)
                     /locus_tag="O0X37_00685"
     CDS             complement(5894..6424)
                     /locus_tag="O0X37_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61476.1"
                     /translation="MNRITKLFLGVSTGVMSLSAAVSAADNTTVVSPIIENSFSVPFW
                     FVAALIVVTIAILVIALTRPTQEALPMNILAFFLSIYVTAASFVSGDYLTPQTLVSGV
                     TTIQDNLTLYNQTSEVIVPIVIHESIGTLFLCIGILTLSLILLLARVFIYFGESATEL
                     DDSIEVPKYDRSLNNR"
     gene            complement(6443..6742)
                     /locus_tag="O0X37_00690"
     CDS             complement(6443..6742)
                     /locus_tag="O0X37_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61477.1"
                     /translation="MPKLGFNPSNVANDKGNASVTAAVAGLISVIVVIVFGIIITGSF
                     TTVATSASLNLDEKWLTALNTTTDIAATSFNLAALIPIAMVAGVVISIVTYMIVR"
     gene            6904..7332
                     /locus_tag="O0X37_00695"
     CDS             6904..7332
                     /locus_tag="O0X37_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61478.1"
                     /translation="MSKDFPTVAGSLWARLATEIGVEASTTTCGYFISIVKAQCVVKR
                     DKLTDKIQAVGAENATTDQVFQLERLSKDLKRLEKLDDDWAEVRKSMKPQPGSTYYLT
                     TTAQIQKISSGVLEIAIRNDLIDIPDAAKFAAYRPEGETW"
     gene            7326..7700
                     /locus_tag="O0X37_00700"
     CDS             7326..7700
                     /locus_tag="O0X37_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61479.1"
                     /translation="MVKPSNMQANVDAGRVYSVAGLPELEVANTLDFRIFDLVRAQTY
                     IVQNITPQDPRPYLNILQQINTIPDPAWRQRVLAKLDELLEDHDFRSVEGHIACIMVS
                     GAITDWQARYRPVVTYNLVGQE"
     gene            7700..8656
                     /locus_tag="O0X37_00705"
     CDS             7700..8656
                     /locus_tag="O0X37_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJK61480.1"
                     /translation="MSEIGRWVQTCAQAFYDDEYAFPTRPQGNFSFGEHIAKILADPV
                     DEQHANIVVVGQSGMGKSTTAVSIAMSIARHLAEIFGDQPEDHFRLDTHVSILDQSTA
                     TNIKRMCATKMYQIYIIDEAADDTPARRAMSMENINNVKFMATVRTSRSCIIRCVQFT
                     SLIDGAILDQATHLIRIVEPHHDEGYNVVKIRTMKVFGDNPRPYKVYMKPNGRDKIVR
                     HIIYAPDESTYARYRGMRKEKADTRILQYADDPVESDTASPRDITRQKCAKAWDDYMN
                     MSHPYKRVSRCALDAGVSPQTLQKWMGENGHTESERQRESSN"
     gene            complement(8840..9382)
                     /locus_tag="O0X37_00710"
     CDS             complement(8840..9382)
                     /locus_tag="O0X37_00710"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HJK61481.1"
                     /translation="MIDIFLIIPVIAAAALIAYIDYETHIVQKRWILLLALCGIPAAI
                     ASWQRVSIAPNPVIEVLPGIFILALISILMCIELDGVQLLGLGGADIKVIAVLTVIFP
                     AQIHWILLLALILGVVFSSAYLYGRNKYFDADITQKITMLGSRVTQNGKCTVVWQPWT
                     MPLCVPIAGSLLIMMYFWIL"
     gene            complement(9508..9711)
                     /locus_tag="O0X37_00715"
     CDS             complement(9508..9711)
                     /locus_tag="O0X37_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61482.1"
                     /translation="MVKRTYRKPSPRTDSKREGAAISRAKSEIRKAKAENKKAATALD
                     RVDRELRTAGRYNKSERRGMKRR"
     gene            complement(9747..10661)
                     /locus_tag="O0X37_00720"
     CDS             complement(9747..10661)
                     /locus_tag="O0X37_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJK61483.1"
                     /translation="MKTQTNKTKVIIYVVMAIITTSVLTAGCISADQIYTVPAHTNLT
                     LHFVAVGQADAIVIQQGDTYAIVDAGKPLTKDPAATDIMTYLKTIQPETIDFLLITHQ
                     DYDHIGYAQDILQEYPTNIFYDNGFVHTSKTYENLMEEVGEQNIPYIVVRGGDSIENS
                     FENVSISVLAPQKLLSDDINANSIILRICYGNQSALLTGDAENPEELLSSGPILSPLH
                     SDLLKVGHHGSAKSSSGDFLNRVSPDIAVISTGKNSYGHPNPQTVNRLIEAVGSENLY
                     LTSGGTVIATTDGIHDWYITQVPATPAT"
     gene            complement(10678..12534)
                     /locus_tag="O0X37_00725"
     CDS             complement(10678..12534)
                     /locus_tag="O0X37_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61484.1"
                     /translation="MSQTLISSGRSRDPVGAFFMVSDSGAVCDLSSLILDGAKLTVLF
                     DKLCHDWNHHGHTGDWAYVRLRRACIAWEKAGYVSISRKSYCSASVELSERPGLFVRA
                     VAAPPSTPPYLIKGLQNSKSLTAARNGLGLDDGSVQTTLPELAAATAAKLNTSEQHAR
                     THLYGMPGGRISWERQQAIKRCMEISDPSLFVRVLVGNSKQIHPDLQKKLDQMHALFA
                     SWKRSVSRKKIVLRKLHAHQLEDPYLILDYKTRFTDNWRAWQNEKTYKNAIKTSLENY
                     QHGVFVTLTSDPQIWMRSKGEQFQRHIKDGNKTYHFDATGRGQNLFVANRHESTAWRK
                     WYEAECHRHGMRIPYIRVVEFQKNGLIHTHILLFGIQWDKPWHEFAQDWGGRYSQGFM
                     NKAYSIENRNGRWVWAHAEQKPDDTKGRDPADYLMKYLRKAQDVPTIKCPDCGSYIPV
                     SEDGKCPFCGAKVKAPHDGRYMYWVCGKRFFTMSQSLRVYDFDEMIERDDSRQTGLYE
                     FLAAPDESLVSSIIRDDRVARSPLIRDVPYIPRIDPEIQEFYRHWPFDDEENRISLPE
                     SESPDVDDDNLADFLGSLSPERIAYEQQLAEEAKLMEERRKRLKRNHEERSG"
     gene            complement(12652..13500)
                     /locus_tag="O0X37_00730"
     CDS             complement(12652..13500)
                     /locus_tag="O0X37_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HJK61485.1"
                     /translation="MILTGDSREILPRLEPETIDCIITSPPYYQIRDYNVSGQIGTEQ
                     TPEDYITTLTGIFAAAKTLLKPRGTLWINIGDTYKNKNLLGIPWKLAFALQNHGWTLR
                     QDIIWAKPNPMPESVCDRCTKSHAYLFLLSKSPDYYFDMDGIQEKARYQKEDSFNTGS
                     QYRYGGKKYSEHPDQFYRTKSGNAYTYTGTRHPRSVWSIPTQPFPGDHFATFPEELVR
                     RCILAGCPPDGLVLDPFCGSGTTGKVAHDLGRRFCGIELNAEYVQMAEKRIGSSRQSL
                     LGVMDM"
     gene            complement(13497..14255)
                     /locus_tag="O0X37_00735"
     CDS             complement(13497..14255)
                     /locus_tag="O0X37_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HJK61486.1"
                     /translation="MRNRPFCRSILARRFPGSVLFDDIRKLTGTDIISCCGGKPTVLS
                     GGFPCQPVSVAGNRLGSSDERWLWPEFIRLVRESRPVWVVCENSPRLSRIPEFSGIHR
                     DLETAGYTVGVFEIPAASAGAPHIRYRAFIVAYSDSNRRKKKKNSCRGSETEKSGSTG
                     ICRYDRCGNNIPANSGGISDTQNSRENDLWSSRNTLSLPDWKNNPSGICRMDDGISPA
                     MDRSRLHALGNAVVPQVARPIFAAISAAERGDLK"
     gene            complement(14393..14635)
                     /locus_tag="O0X37_00740"
     CDS             complement(14393..14635)
                     /locus_tag="O0X37_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61487.1"
                     /translation="MLKRIWDFIDNNSHIILAFVLEIVVIGGCYIWRWKAFFILVVLA
                     IVDTILFLMVIGSPEPDTEWARILANVSHLALMGVW"
     gene            complement(14629..>15029)
                     /locus_tag="O0X37_00745"
     CDS             complement(14629..>15029)
                     /locus_tag="O0X37_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61488.1"
                     /translation="HELSSAGNRSSESQVLQLSAVGISLSDYIRILSNVERSKISKRS
                     VQPMDLECGKCVSHPDICYRSPSGDCVYVAPASRVGEYVIVCRHNISSDRTIRYPPLN
                     SVVFNSREKAEKYLAVYAEIAGLVMERSVC"
BASE COUNT         3225 a         3558 c         4160 g         4086 t
ORIGIN      
        1 aggggtagtt gtaccacatg atcttgatcg tgctgagggc tgcgccgaat gcgtccgata
       61 tctggtatcc ccagctgatg agggatgata ccatggtgtc gagggatacg tatgcggcga
      121 tgatggccag gatgcatacg atgatgatga tccatgcgag tacgattctg tccggccgcg
      181 ggctgttgcc gatacccatg tttatgcccc agacgaggat gccgatgata aatgcgagcg
      241 ggaaccatgt ccagaggagg ttgaggaaat ccattacagg tatgaggtct gtgttgagct
      301 gaatacccgc ttcgtagagg gttttgatcg cggggtcgaa caggatgtat attacactga
      361 caacgaagat gatgacgatc atggcgatcc agccgtaggc cattcttcct tctgccatta
      421 ccgtttcctc cgttttggtt tggtgcgttt accaggtttc cgctgcggtt ctatggcggg
      481 caggatgtcg cctgattttc tgcgagttgg cggtgttgtg ctgaatatgc cgtcgtattc
      541 gtagtatgtg gtttgtggct gcggttttct gcgggtgcgg ttggtcatgc tgccgatgcc
      601 gagggcttcc agagggtcgg tgatgctgag atgttcgatg ttttcgacgc ggatcttgcg
      661 gtaggcggtt ttgcgtttgc gggttgctgc ggattttttg cggcgaggta tgatcggtgt
      721 ggtcttgggg atggtgcggg ggactgcaac cggatagccg ccgcgtggtg cgggtgcagt
      781 gggatacggg ttgacgcgat ggtcgggggt gtatccaccc gggtagatgg ccggggttgt
      841 tgtgcccgga gagtattggg aggtgtatgc ggctgcatat tctgtgtctg ttgggtattg
      901 tgcggggtag taggatgttt cgcggagttg tgcggggtag gggtatgttt ttttcgcggg
      961 tactgggtag ttgtcgtaat ttccggggag gattgcggga tagtatcctt tgtctggtgc
     1021 gagtgatttg tatccggatc cgtatttgtc ctcgggagtc agatcacgcg ggtatctgtt
     1081 ttcgtcgagg gatgttctgc gtgtttttgc gggttttcgg atgttttcga tgatgtcggt
     1141 gttgattctg ttgatgggta tggcgatgtt gatgcctgct gtggtgccga gtacgcggtt
     1201 gatccgtttt tgtactgcgg cagattcagc tgcctgcatt gcctttcctg cgaagagttt
     1261 ttccatcttt actgctgttt caaaccgcgt aaagtcgagc ttatcagcgt cggcaagggc
     1321 ttttgccatg ccggacgggc gggatatgag gtaggagagc gggtcggatt tgtcaaggcc
     1381 ggtatggcgg gcgatggcgt cggctatctg tttttgttgt ttgggggtga atgcagccca
     1441 ttgttttcga tctgctacga gtcgtttgta tgcgtcaggg tagtcctgca gcagcacttg
     1501 tttgagggca gggcgttttt ccagtgcttt gagcgggtcg tcggcgcctt tcagcagtga
     1561 gagggctttt gcctgtccgt gcgggattgt ctgcgggtag tcggtgtcta cgattttgcc
     1621 gaggtcgtgc gcatatgctg catattcgag tgcggtttta tcgatgtttt tgccatagat
     1681 ttttgggtat ttttcgatgt ttgccagtgc tgtcctggcg acgctgctga cgtgtgcggg
     1741 gccgtgcgag ctgaagtcat aggtggtggg gagtatgcgg gtttcctgct ggatttgttt
     1801 gagtgctgcc tgttctccgt aatagtcgag gagggttttg gggacaaata tacggtctcc
     1861 ggatctgcgg aatggtgctt tccagtccac atagaactgg ttgtttttca ggattctttc
     1921 gccccatgcg gagaggaact cttttgcgat ggcggtgttg gtctgggcgt ttgtcatgtt
     1981 ccagacgggt ctgccgtccc gtgagaatcc tgcaaagtat gcccgcacct tctgcgtgga
     2041 tgcaccaccg gcggggatga ttgcaacctc ggcttctttc tcatctaatt ttttgccgaa
     2101 ttgtttgttg taggatgcca ccacaacggg tttgttttct gccgcggcga tggctgcgcc
     2161 gcgttcctcg agtgtatcgt agaatttccc atataccggt gagtttgcca cttcgcgata
     2221 gacggccatt gcttcgtccg gtgagaccgg gactttgtcg gcctgcagcc ggaacacgcg
     2281 aactttgttg gatccgtcca gtttccctaa tcccgtccag ctcggtgcat acccgtgatc
     2341 gggcgtggcg aagaggatct cccgttttcc cggttccgga tttttaggat aggtctcagg
     2401 attctttgct tcgagtgtga aatcacgaac aaaaaaaccc tcagacggag attttgccgc
     2461 cggaacacga cggatatagg atcctgtcgg tcctccttcg attacctcgg agagtgcccc
     2521 cagatcagat attgcgcctg cggcaggacc tttagggctt tcgattccca catcatacag
     2581 accgatgcgt ctgcctatcc aaccgggcgc tgctttgagt gctgatcctg cggcagttcc
     2641 taacggcagt gtgatcagtg tttcaccggc aagttctccg aggaatcttg ccgggttttt
     2701 ctcggcctcc tttgccattg cctcaaacgc agaggttccc gcatacacga catttccggg
     2761 agtgatgaga tccggatttt tcaccactgt ttctgcgaga gttgcggcgc tgcctgcgct
     2821 gcggacgacg ttttccggtg cggcagacag gccggattca aagtcaaaga atgcccgcgt
     2881 gtttgctttc gatccggatg ccagatcaat acgcagatca gcacccggca gattcttgag
     2941 aatgccgttg cggttcagat cgatgtactt gtctgcccat gcgtcgtaga gatttgccgc
     3001 acccgtgaat gctgattcga gcggttttga tactgagtcc cgaatagcat tccccagatc
     3061 cgttgctgcg gaggcgatac ctttcgacca gtcgagcccg gatatttccg gattctgaac
     3121 aatttcacgc gataccggat cccgatgtgc aataccaaga ttttccagga tagattctgc
     3181 ctgtttttcc gagatgggtt cagtaggtga cagccagagc ggttgttcat atcccggcac
     3241 cgataccggg aactgcaggg tgtctgtgcg gtatacctcc tcccggacgg gttcgcccag
     3301 cagggggatg tatgtattat actgcagtga ttcgagtttg tcccctttgt ttgccgtgcc
     3361 ggacacccgg aactcgatgt cgcggcctgt cacaggatcc ggaagagaga tgcggatgga
     3421 tcccactaca tctttctgtt ccgccggtgt gagctggcga tatccattca cgctcgacag
     3481 atacatcggg gcattcagat cgacggatcc gttttgtccg ggcgacatga acaggtaggt
     3541 gttttggttt gcagcaccct cgattgcttt tgcgtaatcc cggagtgtgg ggtttgtctg
     3601 ttccgcggcg gatcggttct ggtaggctgt ccagtcggca cgttctgcgg ctcgcacctg
     3661 gtcggtaaat gtgggttgag atgcctggtg tatcttgaac tcagctatgt ccctgccaag
     3721 atcttcaaag gatttatacc ccgtatttac catgaaattg tgtaaatttg caacatactc
     3781 actttgtgcc gcgttgtact ccactcttgc ggcgttttgt gctgcatctg cggcaattcg
     3841 ttcgggtgca acccgcggca tatacacttt attctcaaac gtgaggggtg tatcacggaa
     3901 ttttaggtat tcctgatact ctttctcaag gagttctttg gattccgcag agttcggatc
     3961 tgcgagcact ccagctctcc caatttttag aggatcctga acatatgcct tatacattgc
     4021 ctcctttctg gattcgttcg cgcctaaatt gatcattccc agagagaaag catgtgcaag
     4081 atcttcggta tctgctattt ttccctccca tacaaaatcc cggccttcgt tgcgtgctgc
     4141 gttgtatgcc tgttgcacat caacgagatc ccgctcatag tttcccgttc gctctccgct
     4201 aaaataactg gttactccgg catttgcgcg ttcgcgctga taatagagat aatcccgcgc
     4261 ccggtgaagt ttgtcgcctt caaacagttg atcccgctcc cagtcggcga accgctgagt
     4321 ttctgcgtcc cgtgcgttgg cagcgagatc cgcacctctt ggagatcgga gactcgatat
     4381 ttccgtacca cgccggttta ccatggtcat ttcgggtgct tcggtggatc tgaactcatc
     4441 tggcagatca tgatactcca ccagcgcatc cgtatattcc cgaacatcac gctcacgctt
     4501 tgcataggct gcgctcgcct gctgagctgc aaccgccgcg gctgcctgct gctgtacgcg
     4561 tgcttcggtt gggttgatgg tggcaccagc tcccgcgttt cctgcatcgg gtgcgagact
     4621 tgcacctccg gcaccgcctg acggctggat tacgggagct cctgccatgt tacatcactc
     4681 ccatgccctt cattgccatg acagcgatta agattccgac aatccctatg acgatcagcg
     4741 gcagcatgcc tttcagatct gctgcggatg atttccccgt agctgcctgc acatactgct
     4801 cggatgcctc ggcgatgccc tcggagatgg cctgcgagat gatctccgga gatccctgtg
     4861 cccagtcaac cagttttttc acattcattt tgccgcagag cggatagaag acggtaaaat
     4921 catactcagg atgtgcccgc agaaattcag acgcaaactg gtatgattga ttggatatcg
     4981 ggatttttgc ggatgtggca gagatggcat tcagaatcag caccatatcc tgcagagatc
     5041 cgatgccgag ccggtcgagt tcatcggtca tggcgacaaa ttcggtttcg gacattatgc
     5101 caacctgatt gaggacgatg tcgcagcggc agttgccaag attgtaggat ccgttcagtc
     5161 cgagttttac aaaccgcccg attctatccc tgatgattgc cataccgtta tgaaattctg
     5221 ccggaattgg acggattgcg ttcgtttttg agatttctaa gatacagtat ttcgatttcc
     5281 cgaacgatgc tttcagatac gggattacca ggtcttttgc gagcagtgca gggaataacc
     5341 ccacacacat agagatcatt gccaccatgg cgagtgtggc gtaaaatgtg gtgtcgtcaa
     5401 gtacaatcat ctgtggcctc cgaaggcatt tttcatccgg tcaatccaac cggactgctt
     5461 ttcatctccg gacggcatcc ggatctgctg ctgctgatgc gtgatagtgg taaatgtgct
     5521 gatgctgcag tttctctcat atccgtcctg agcctttgcg agcaggatga gattcagata
     5581 gctgatgaca gcctctctgt acatcgtcat gccgctatgc gggttgcata gtttgtagag
     5641 cgtgagagac tgccgcgccc taattttcca gatagccatg tcctttttcg agaataccgg
     5701 catgtattgc tggaagattg cgagcagtgc agggaaatcc tgtttcagat cggcgggtat
     5761 ttcatcctga ccggagatca gccgcataag cacttcgctg gccatcggat caggcggcgg
     5821 gggagttgcc cccacatacg gtgcgtcgcg gtctgcctgt gcctctttga tgaggtctcg
     5881 cagccgcgtg atgtcatctg ttgttaaggg atcggtcata tttgggtacc tcgatagagt
     5941 cgtcgagttc ggttgcggat tcgccaaagt agatgaatac ccgtgccagc aggaggatca
     6001 gcgaaagtgt caggatgccg atgcagagaa agagggttcc gatagactca tggatgacaa
     6061 tcgggacgat cacctctgac gtctgattat acagcgtgag attatcctgg atggtagtca
     6121 ctccggatac gagtgtttga ggtgtgagat agtctccgga gacaaagctg gctgcggtaa
     6181 catagattga caggaaaaat gccagaatat tcattggcaa agcttcctgt gtcgggcggg
     6241 taagtgcgat gacgagtatt gcgattgtta caactatcag tgcggcaacg aaccaaaacg
     6301 gcacagaaaa ggagttttcg ataatcggtg aaacaacagt ggtgttgtct gcagcagata
     6361 ctgctgcgga caggctcatt acccctgttg atacaccaag gaacagtttg gtgatgcgat
     6421 tcatgacaaa acaccccacg gatcagcgga cgatcatgta tgttacaata ctgatcacaa
     6481 cacccgccac cattgcaatc gggatcagag ctgcgagatt gaacgatgtt gcggcaatgt
     6541 ccgtagtagt gttcagagct gtcagccact tctcgtcgag gttgagactg gcactggtcg
     6601 ctacagtcgt gaaggaaccg gtaatgatga taccaaagac gattacaacg atgacggaaa
     6661 taagtccggc gacggcagcc gtaacggatg cgttaccttt gtcattcgca acattggacg
     6721 ggttgaatcc aagctttggc atcttcatat acccattgcg gggcatacac agagacattt
     6781 tttatcttac acctcctctg tgcgtattgt acgctctcat actaaataaa ttttacttta
     6841 gttataacaa aataactttc tttaagagaa aaaattattt ctgtaaaaaa aatattttta
     6901 ctcatgtcaa aagactttcc aacagtagcc ggatccctgt gggcacggct ggcaacagag
     6961 ataggagttg aggcatcaac tactacttgc ggttatttta tcagcattgt gaaagcccag
     7021 tgcgtggtaa aacgagataa gcttactgac aaaatacagg cggtcggtgc agagaatgcc
     7081 acaacagacc aggtattcca gctcgaacgg ctcagcaagg atctgaagcg tctcgaaaaa
     7141 ctggatgacg attgggctga agtccgaaag agtatgaaac cccaaccggg atctacctat
     7201 tatctcacca ctactgcgca gatacagaag atatcttccg gagttctgga aattgcgatc
     7261 cggaatgacc tgatagacat ccctgatgct gcaaaatttg cagcatacag gccggagggt
     7321 gaaacatggt gaagccgtcc aatatgcagg caaatgtgga tgcaggccgt gtgtattcgg
     7381 ttgccggatt gccggagctg gaggtggcga atacccttga tttccgcatt ttcgatctgg
     7441 tgcgtgcgca gacgtacatt gtgcagaaca tcacgccgca agatccgcgc ccctatctga
     7501 acattctgca gcagatcaat acgattcctg atccggcatg gcggcagcgt gtgctggcca
     7561 agctggatga gttgctggag gatcatgatt tccgatcggt agagggacat attgcctgta
     7621 ttatggtttc aggagctatc acagactggc aggcacgata ccggccggtg gtaacataca
     7681 atcttgtggg gcaggagtaa tgtctgagat cggccgctgg gtgcagacgt gcgcacaggc
     7741 attctatgat gatgaatatg catttcccac gcgcccccaa ggaaattttt cgttcggaga
     7801 gcatattgcc aagattctgg cggatcctgt ggatgagcag cacgccaata ttgtggtagt
     7861 gggacagtcc gggatgggga agagcacgac ggcagtgtcg attgcgatgt ctattgcccg
     7921 gcatctggca gaaatttttg gtgatcagcc cgaggatcat ttccggcttg atacgcatgt
     7981 atcgattctg gatcagagta ccgcgaccaa tatcaaacgc atgtgcgcca caaagatgta
     8041 tcagatatac atcatcgacg aggctgcaga cgatacaccg gcacgacgtg cgatgtcgat
     8101 ggagaacatc aacaacgtga agttcatggc tactgtccgg acatcgagat cgtgtatcat
     8161 acggtgtgtg cagtttacca gcctgattga cggcgctatc ctggatcagg caacgcacct
     8221 gatacggatc gtagagccgc atcatgatga gggttacaat gtagtgaaga tacgcactat
     8281 gaaggtgttt ggcgacaatc cccgaccata caaggtatac atgaaaccga acgggcggga
     8341 taagatagtg cggcacatta tttacgcacc ggacgaatcc acctatgccc gctatcgagg
     8401 tatgcggaag gagaaggcag acacacggat cctgcagtat gcggatgatc ctgttgagag
     8461 tgatacagca tcacctagag atatcacacg ccaaaagtgt gcaaaggcat gggatgatta
     8521 catgaatatg tcccatccat acaaacgggt tagcagatgt gcattagatg caggtgtgtc
     8581 cccccagaca ttgcagaagt ggatgggaga gaacggacat acggagagtg agaggcagag
     8641 agaaagcagt aactaaaaaa caaaaaaata ttttttccca aatacactta gtttagacta
     8701 tagatatact gggaagcaaa aaaacacgaa cacgacagcc accgcccggg gactgtccaa
     8761 aagagccctt ttttgaacgg atttacgacc ccagttaagg cagttatcac cccagttaat
     8821 gtggatttta aaaaagagat tagaggatcc agaagtacat catgatgagc agggatcctg
     8881 cgataggcac acacagcggc attgtccatg gttgccatac cacagtgcat ttgccgtttt
     8941 gagtgactct gctgccgagc atggttattt tttgtgtgat gtctgcatca aagtacttgt
     9001 tgcggccata gaggtatgca gatgagaata ccacacccag aatgagtgcc agcagtaaga
     9061 tccagtgaat ctgtgcggga aagatgactg tcagaaccgc gatcactttt atatccgctc
     9121 cgccgagtcc gagcaactgc acaccgtcaa gctcgataca catgaggatg ctgatgagtg
     9181 caaggatgaa gatgccagga agaacctcta tcacaggatt tggtgctatg gacacccgct
     9241 gccagctggc aatagcggca ggtataccgc agagtgccag cagtaagatc catcgttttt
     9301 gtacgatgtg tgtttcatag tcgatatatg caatcagagc tgccgctgcg attaccggta
     9361 ttatcagaaa tatgtctatc atggtcggtt ttgcaaaaaa ttaggttggt cgtcgtgctg
     9421 ttcacttgcg gcatccacgc cgcgatgttt ttgttttccg ttttccggag gtttttttgg
     9481 ttgtttttct ttttgcagcc attgttatca gcgccgtttc atacctctgc gttcagattt
     9541 gttgtatctg cccgcagttc gtagttctcg gtcgactctg tcgagagcgg ttgcagcttt
     9601 tttgttctct gcttttgcct tcctgatttc ggattttgcc cgagagattg ctgccccttc
     9661 ccgcttgctg tcggtgcgag gcgagggttt tctgtaggtt cttttaacca tacaaacaag
     9721 tatattttct ttaatacaag taaactttat gtagcaggtg ttgcaggcac ctgcgttatg
     9781 taccaatcat gtatgccgtc ggtggttgcg atcacggttc cgccggaggt gagatagaga
     9841 ttctctgatc ctacggcttc gatcagtcgg ttgactgttt ggggattggg gtgtccatag
     9901 ctgttttttc cggtggagat taccgctata tccggagata cccgattcag gaagtcccca
     9961 gaagaggact tcgcagatcc gtgatgccct acttttaaga gatcgctgtg taacggtgat
    10021 aatatggggc cggatgagag taattcttcc ggattttctg cgtcgccggt gagtaaggcg
    10081 gactgattgc cgtaacagat gcggagtatg atcgagtttg cgttaatgtc gtcagacaat
    10141 agtttctgtg gagcgagtac agagattgac acattttcaa aggagttttc gatggagtca
    10201 ccgcctctaa ctacgatgta cggtatgttt tgttccccta cctcttccat gaggttttca
    10261 taggtttttg aggtgtgcac gaacccattg tcatagaata tatttgttgg gtattcctgc
    10321 agaatgtctt gagcgtaccc aatgtgatcg tagtcctgat gcgttatgag gaggaagtcg
    10381 atcgtttccg gctggatggt ttttaggtag gtcattatgt cggttgctgc aggatccttg
    10441 gtgagtggtt tgccagcgtc gacaattgcg taggtatctc cctgttggat tacgatcgcg
    10501 tctgcctgtc cgacggctac aaagtgcagg gttagattgg tgtgtgccgg tacggtgtag
    10561 atctgatcgg ctgagatgca accagcagta aggacggatg tggtgatgat ggccatcacc
    10621 acataaatga taaccttggt tttgtttgtt tgagttttca tgattttttt ccttttttta
    10681 gccgcttcgc tcttcatgat tccgtttgag acgtttgcgg cgttcttcca tcagctttgc
    10741 ctcttcggca agctgttgtt cgtatgctat acgctcaggg gacagagatc ccaggaaatc
    10801 agcaagattg tcatcgtcca catccggact ttccgactcc ggcagagata tccggttttc
    10861 ctcgtcgtca aacggccaat ggcgatagaa ctcctgtatt tccggatcga tccttggtat
    10921 gtaggggaca tcccgaatca gcggtgatct ggcaacccgg tcatcacgga tgatacttga
    10981 taccagcgat tcatcaggag ccgccaagaa ctcatagagt ccggtttggc gcgaatcatc
    11041 acgctcgatc atttcatcga agtcgtacac ccgcaacgac tgcgacatcg tgaaaaaccg
    11101 tttgccgcat acccagtaca tatacctgcc gtcgtgcggt gctttcacct ttgcaccgca
    11161 gaatggacat ttcccatctt cacttaccgg gatgtatgac ccgcagtccg gacattttat
    11221 ggttggcaca tcctgcgctt tccgcagata tttcatgaga tagtccgcgg gatcgcgccc
    11281 tttggtatca tccggttttt gttctgcatg tgcccatacc cagcggccgt tcctgttttc
    11341 tatcgagtaa gccttgttca tgaatccctg agaatatctg ccgccccaat cctgcgcaaa
    11401 ctcatgccat ggtttgtccc actggatgcc gaataacaaa atgtgcgtgt gaatgagccc
    11461 gtttttctgg aattcgacga cgcggatgta cggaatacgc atgccgtgcc ggtgacattc
    11521 ggcctcatac cactttctcc aggcggtact ttcgtgtctg ttggcaacga acagattttg
    11581 gccgcggccg gttgcgtcaa agtgataggt tttgtttcca tctttgatgt gccgctggaa
    11641 ctgttctcct tttgatcgca tccatatctg cggatcggag gttaacgtga caaatacccc
    11701 gtgttggtaa ttttccagac tggtttttat ggcgtttttg taggtttttt cgttctgcca
    11761 ggcacgccaa ttgtcagtga agcgggtttt gtagtcgagt atcagatacg gatcttcgag
    11821 ctggtgtgcg tgcagttttc gcaggacgat cttttttcgg gacacgctgc gtttccagct
    11881 tgcgaacagt gcgtgcattt gatccaattt tttctgcaga tccggatgga tctgttttga
    11941 gttgccaacg agcacccgca cgaacaacga tggatccgag atttccatgc atcgtttgat
    12001 tgcctgctgc cgttcccagc tgatgcggcc ccccggcatt ccatagagat gagttcttgc
    12061 atgttgttca ctggtattga gcttggccgc ggtggctgct gcgagttccg gcaaggttgt
    12121 ttgtactgat ccatcatcca aaccaagccc attccgagcc gctgtcaaag atttggagtt
    12181 ttgcaaaccc ttaatcaagt aagggggtgt actggggggt gcggcaactg cacggacaaa
    12241 taacccagga cgttcggata attccacact ggcgctgcag taggatttcc gtgatatgga
    12301 tacgtacccg gctttttccc acgcaataca ggctcttctc aggcgtacat atgcccagtc
    12361 tcctgtatgg ccgtgatggt tccagtcgtg acacagtttg tcaaacaaca cggtgagttt
    12421 tgcaccatcg aggataagag aggatagatc acataccgct ccgctgtcgc taaccatgaa
    12481 gaatgcgccg accggatcgc gagagcgtcc gctggagatg agggtttgcg acatggttcg
    12541 gatttaaaaa atgattttgt gtgttttttg gctggtgtga tattgggggt tagagagtgg
    12601 ggacgtaaga ggtgagattc tcagaggatc gttactgcaa gggagtgttt gttacatgtc
    12661 catcaccccc agcaagctct gacgggaaga tccgatccgc ttttccgcca tctgcacata
    12721 ctctgcattc agctcaatgc cgcagaaccg tctgccgaga tcatgagcca cttttccggt
    12781 agtgccggat ccgcagaacg gatccaatac aagcccgtcg ggcggacagc ctgcaaggat
    12841 acagcggcgt acgagttcct cgggaaatgt tgcgaagtgg tcgcctggaa acggttgtgt
    12901 cgggatgctc cacacggaac gcgggtgtct ggttccggtg taggtgtatg cgttgccgga
    12961 tttggtgcga tagaactggt cgggatgttc cgagtatttt tttccgccgt accggtattg
    13021 tgatccggtg ttgaaggaat cctctttttg ataccgtgct ttttcttgga tgccgtccat
    13081 gtcaaagtag tagtccggcg atttgctgag caggaacaga tacgcgtggg attttgtgca
    13141 tcggtcgcag acgctttccg gcatggggtt tggttttgcc cagatgatgt cctgccggag
    13201 tgtccagccg tggttctgga gggcaaatgc gagtttccac gggattccga ggagattttt
    13261 gtttttgtag gtatccccga tgttgatcca gagtgttccc cggggtttaa gcagggtttt
    13321 tgctgctgcg aagatcccgg ttagggttgt gatgtagtct tcgggggttt gttcggtgcc
    13381 gatctggccg gatacgttgt agtctctgat ctggtagtag ggcggggatg tgatgatgca
    13441 gtctatggtt tcgggttcga gccgtgggag tatttcgcgg gagtcgccgg tcaggatcat
    13501 ttcagatcac ctctttccgc cgcggagatc gcggcaaaga ttgggcgtgc aacctgcggc
    13561 acaactgcgt tgccgagagc gtgcagtctg gatctgtcca ttgccgggga aatcccatca
    13621 tccattcgac aaattccgga tggattgttt ttccaatcag ggagggatag agtgttccga
    13681 gaactccaca ggtcattttc ccgtgagttt tgtgtatctg agatgccgcc ggagttggca
    13741 gggatattat tcccgcatct atcatagcgg catataccgg tactcccgga cttttccgtc
    13801 tcagatccgc ggcagctgtt cttcttcttc tttcgtctgt tgctgtcgga gtaggcaaca
    13861 atgaacgccc tgtatctgat gtgcggagct ccggcagatg cagccggtat ctcaaacacc
    13921 ccgaccgtgt atcctgccgt ttccaggtcg cggtgtatcc cggagaactc cggtatgcgg
    13981 ctgagccgag gagagttttc acaaacaacc caaaccggcc gggattcccg aaccagtcta
    14041 atgaactctg gccagagcca tctttcgtcg gatgatccaa gccggttccc ggcaacgctg
    14101 acgggctggc agggaaatcc cccggacagg acggtaggct ttcctccgca gcaactgata
    14161 atgtcggttc ccgtgagttt tctgatgtcg tcaaataaga cggatcccgg gaatctgcgt
    14221 gcgagaatgg aacggcaaaa gggtcgattt cgcactgcac ttttgtgatg aatccggcag
    14281 attcggctgc aaggtcaagt ccccctatgc cgctgaagag cgatgcgtgg gtaagctgtg
    14341 tcatttccga gcctcctttt ctgctgcgag aagttctgag atgttcatag cgctaccaca
    14401 cccccattaa cgccagatga gaaacatttg caaggattct cgcccattcg gtatctggct
    14461 ctggcgatcc tattaccatt aagaacagga ttgtgtcgac tatcgcaaga accactaaaa
    14521 tgaagaatgc cttccacctc caaatatagc acccgccaat tacaacaatc tccagaacaa
    14581 atgcaagaat gatatggcta ttgttatcaa taaaatccca aattcgtttc agcatactga
    14641 cctctccatc actaatcctg ctatttccgc atacactgca aggtattttt ccgctttttc
    14701 ccgcgaattg aacactactg agttcagcgg aggatatcgg attgtgcgat cgctgctgat
    14761 gttgtgccga cagacgatca catattcgcc taccctgctc gctggcgcca catacacaca
    14821 atcaccggat ggtgaccggt aacagatgtc ggggtggctt acacatttgc cgcattccag
    14881 atccattggc tgcacgctcc gttttgatat tttactgcgt tcgacgttcg acagaatccg
    14941 aatatagtcg gatagagaga tacccactgc tgacagttgc agcacctgac tctcggaaga
    15001 tcggttaccg gcggaggaaa gttcatggc
//
LOCUS       DALUAJ010000036         3458 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_7010,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000036 DALUAJ010000000
VERSION     DALUAJ010000036.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7010"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="O0X37_00750"
     CDS             <1..1207
                     /locus_tag="O0X37_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methanogenesis marker 3 protein"
                     /protein_id="HJK61489.1"
                     /translation="CLGCGGYDSGTSYLMFSRLQHMGDHGAASGGAVLGTVISGLGIM
                     NRWRNGDRITKIEHVFTSVDKTNAVVTTDLSLEVEDGMQIFSEILINAEGYKEDHSAI
                     DTSCTKSVEHLLFALRNSEYLIDRTASTYIRDHTEGKLAVPMELQKPRREGTVTVRTS
                     GKSSGAIYIYTKDVPSNQHHTRTGTVVRGIELARFANVGTKLSVKVVPEQLDLRGLPL
                     GEAVARAKARGLRVLADNRDVDGRVVIDQNPATTLEVLKEGKVALSTVALEDVIDITL
                     DYEHAPRTVDLFRRVTGLKIYAIGSMPFFYNIDDEMYLFKPKFASNVNIIPENVPKQP
                     VPPYSLAVTNDARKARGMTGVRSVANEEYGPTGEPFEGTNVIGTVLDIDKLPLIKDGS
                     TVFIREVKP"
     gene            1204..1662
                     /locus_tag="O0X37_00755"
     CDS             1204..1662
                     /locus_tag="O0X37_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 6 protein"
                     /protein_id="HJK61490.1"
                     /translation="MKPYVPQYVGTVTKYVFVDSPNTTPQDIATAAYEIAQGVMIKET
                     CFGCQITGSPEDVERIIAHLRKLDPYRIYVKDRGFPPGDARRCRADLGGARPGYLGHE
                     YEMGLVRYIAYGLQETDRMTPEELSAAAGREMPTPEALDIDILESLMKEE"
     gene            1668..2114
                     /locus_tag="O0X37_00760"
     CDS             1668..2114
                     /locus_tag="O0X37_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 5 protein"
                     /protein_id="HJK61491.1"
                     /translation="MAKVFIYPATSLILSDLVARFGHQPLGSAISIREHIQTPGFDSP
                     PIQMTSDDPQKGLKWAAVEVPSGIRGRMSLYGPLVEEAEAAIIIDEPDLSFGCMGCAR
                     TNELLAFMLREKDMPRLELRYPTSKEEGVTFVAAIKEFLKNLEGQQ"
     gene            2111..3364
                     /locus_tag="O0X37_00765"
     CDS             2111..3364
                     /locus_tag="O0X37_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 15 protein"
                     /protein_id="HJK61492.1"
                     /translation="MSEQPVRIAQITCGAEYSGIQNEITTAAETVGGQMFYPDVALKD
                     VKRAYKDFGLPVKSPDLKLAIARAQAIVEGRIEADGIFIASCFRCAEAAIVRNELRRY
                     IVENSRIPVVSYSFNERTGASTLLTRLEALTTIARRRSLMAREVQVGITMGVDSGSST
                     TKCVIMQDNEIIGTGWRPTTEVIKSADEVVALALEESGLKISDIEAIGTTGYGRFLVG
                     KHLNADLVQEELTVNSKGAVYLADAQKGFATVIDIGGMDNKAISVNDGIPGSFTMGGI
                     CAGASGRFLEMTSKRLGVDITELGALSMKSEGGEDVPMNSYCIVFGTQSLVNALAAGY
                     KREDVAAAACHSVAQQVYEQQLQEVDIKEPVIMVGGSSLIQGLVRAMGRMLNTEVIVP
                     HHSQYIGATGGALLASGFIDKKKEA"
BASE COUNT          864 a          865 c          900 g          829 t
ORIGIN      
        1 ctgtcttgga tgcggtggtt acgactctgg gacttcctat ctgatgttct cccgccttca
       61 gcacatggga gatcacgggg cagcttctgg aggagccgtt cttgggacag tcatctctgg
      121 tcttgggatc atgaaccggt ggagaaacgg cgatcgtatt acaaagatag aacacgtttt
      181 cacctcggtt gataaaacca atgctgtcgt aaccactgat ctctcccttg aagtcgagga
      241 cggtatgcag atattttccg agattctgat caatgcagaa ggctacaaag aggatcacag
      301 tgcgattgat acatcctgta ccaaatcagt ggagcatctt ctgtttgcat tgcggaactc
      361 tgaatacctc attgatcgga cggcatccac ctacatccgt gatcacacgg agggaaaact
      421 tgctgtccca atggaactgc aaaaacccag acgtgaagga actgtcacag tacgaacttc
      481 gggaaaaagc tctggcgcca tctacatcta tactaaagac gttccaagca accagcacca
      541 cacccgtacc ggaaccgttg tccgtggtat agaacttgca cggtttgcca acgtcggaac
      601 caaactgtcc gtcaaagttg tgccggaaca gcttgatctc cgtggtctcc cgttagggga
      661 agcagttgcc cgtgcaaagg cacggggact tcgtgtactt gccgataacc gtgacgttga
      721 tggacgggtt gttatcgatc agaatcctgc aacaaccctt gaggtcctca aagaaggaaa
      781 agttgccctt tcaaccgttg cccttgagga tgtcatcgac atcacactgg attatgagca
      841 tgctccccgg acggttgatc ttttccgccg tgttaccggc ctcaaaatat atgcaatcgg
      901 cagtatgccg ttcttctata atattgatga tgagatgtac cttttcaaac cgaagtttgc
      961 ttcaaacgtc aatatcattc ctgagaacgt tcccaaacaa ccggttcctc cctactcact
     1021 tgcggttacc aatgacgcca gaaaagcccg cggtatgacg ggtgtacgta gcgttgcaaa
     1081 cgaagagtac ggaccgactg gtgagccgtt tgagggaacc aatgtcatcg gaactgttct
     1141 tgacatcgac aaacttccgc ttatcaaaga cggctcaacc gtctttatcc gcgaggtgaa
     1201 gccatgaaac cctatgtgcc gcagtatgtt ggaacggtta ccaagtatgt attcgttgac
     1261 tctcccaaca ccactccgca ggatattgca accgcagcat atgaaattgc tcagggagtc
     1321 atgatcaagg agacctgttt tgggtgtcag atcaccggca gccctgagga tgtggagcga
     1381 atcatcgctc acctccgaaa gctcgatccc taccgcatct atgtgaagga tcgcggtttt
     1441 ccaccgggag atgcacgcag atgccgtgct gatctcggag gtgcccgtcc cggttatctc
     1501 gggcatgaat atgagatggg gcttgtgcgt tacattgcct acggattgca ggaaaccgat
     1561 cgtatgaccc ccgaagaact cagcgcagca gccggtcggg agatgccgac accggaagcg
     1621 cttgatattg atattctgga atctctcatg aaggaggagt aaacaccatg gcaaaagtat
     1681 tcatctatcc ggcaaccagc cttatcttat ccgatcttgt ggcacgcttc ggccatcagc
     1741 cgctcggttc ggcaatttca attcgtgaac atattcagac acctggattt gactcccccc
     1801 cgattcagat gacatccgat gatccgcaga aaggtctcaa gtgggcagcc gttgaagtgc
     1861 cgtccggtat tcggggacgc atgtcactgt atggtcctct tgtagaggag gcagaagctg
     1921 cgatcatcat cgatgagcct gacctttcct tcggctgcat gggctgtgca cgaacgaacg
     1981 aacttcttgc cttcatgctc cgcgaaaagg atatgccgcg gcttgagctc cgttatccaa
     2041 cctcgaagga agagggagtc acgtttgttg ccgcaatcaa agaattcctg aaaaatctgg
     2101 agggacaaca atgagcgaac agcctgtcag aattgcacag ataacctgcg gtgcagagta
     2161 cagcggtatt caaaacgaga ttactactgc cgcagaaacc gttggcggac agatgtttta
     2221 tcccgacgtt gcattaaagg acgtcaaacg tgcctacaaa gactttggtc tgccggtaaa
     2281 aagccctgac ctgaaacttg ctattgcccg tgcacaggct atcgttgaag gccgtatcga
     2341 agcggatggt atcttcattg caagctgttt ccgctgcgca gaagctgcga ttgtcagaaa
     2401 tgaactccgg cgatacatcg tggaaaactc tcgtatccca gttgtgtcgt actcattcaa
     2461 cgaacggacc ggtgcgtcca ctcttcttac cagacttgaa gctctgacga ccatcgcccg
     2521 cagacgcagc cttatggcgc gtgaggtaca ggtcggtatc acgatgggtg ttgactccgg
     2581 atcttcgact accaaatgcg tcattatgca ggacaatgag atcatcggta ccggctggag
     2641 accaactact gaggttatca aaagtgcgga cgaggttgtt gcgctggcac ttgaggagtc
     2701 cggtctcaag atctctgaca ttgaagcaat cggtactacg ggttatggtc gtttcctcgt
     2761 tggcaaacac ctgaacgctg accttgtaca ggaagaactg acggtaaact ccaaaggagc
     2821 cgtttatctt gccgatgccc agaaaggttt tgcaacggtt attgatatcg gcggtatgga
     2881 caacaaggca atcagtgtca acgatggtat tccgggttcg ttcacaatgg gtggtatctg
     2941 tgcaggagct tccggtagat tcctggagat gacctccaag cgccttggtg ttgatatcac
     3001 cgagcttggt gccctttcca tgaagagtga aggcggtgag gatgtgccga tgaacagtta
     3061 ctgcattgtg tttggaaccc agtctttggt aaatgcactg gctgcaggat acaaacggga
     3121 ggatgtcgcc gcagcagcct gccacagtgt tgcacagcag gtgtacgaac aacagcttca
     3181 ggaggttgac atcaaagagc cggtcattat ggttggcggt tcatccctga ttcagggact
     3241 tgtccgtgcc atgggtcgca tgcttaacac tgaggttatt gttccgcatc actcgcagta
     3301 cattggtgcg accggcggag cccttttagc atccggtttc attgataaga aaaaggaggc
     3361 atagatggca ggtttagact acttcgcggt tgaatccacg gaggatggtg ccgacaacta
     3421 caataaaatc gcaacaaccg tcctgcagga tcataacc
//
LOCUS       DALUAJ010000037         3797 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_7135,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000037 DALUAJ010000000
VERSION     DALUAJ010000037.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7135"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="O0X37_00770"
     CDS             complement(<1..740)
                     /locus_tag="O0X37_00770"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HJK61493.1"
                     /translation="MHKHCNDPVKQFIPRAGMTVNELVLEMGGAGAYNGGSLYHAVDI
                     YEKMLSDPDMTKFFGLSGAMVPAGMGGIVSTLIEKGHIDILTSTGANLTHDLIEAIGC
                     HHYHGQAECDDLELRKEEINRIYDVFLPNEAYEELESFIQDVYSSLPEQPITISKLLR
                     TIGEQLDTGILATAAKHDVPIYCPAFQDSVLGLQYWMYSQFHKKTILDAIGDMHDLLN
                     TAFSVKKAGAVLVGGGVPKNFTLQTMLMT"
     gene            complement(887..1807)
                     /locus_tag="O0X37_00775"
     CDS             complement(887..1807)
                     /locus_tag="O0X37_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61494.1"
                     /translation="MTLEEKITFYSREDAAEFQKYLKERDCSARISVEHTFSGTPYFE
                     GTIAAFEELISKLLAKEDDSDLAQIKTDLLSREETLAEFFSTHKVGDVLTDATPAQLL
                     AQLESIDADSNEDIQKTATEKFVNSLMILGTLEDNELLKVVGETPEYMLTGVKDPKEL
                     RVMYAYGDLGGIAPEDLDGSGITSHIRTSSTTGYVVTTGAEIVLAQGIDDMSDVLDHL
                     DVDDDEAGRFVDAIFFKQALIAKIHDLVSGGITTEAGLLEAFEAPAFPLGETEDVISF
                     DLSADYLSGVINDLRKLGFLKGRDGKIKSC"
     gene            complement(1909..3144)
                     /locus_tag="O0X37_00780"
     CDS             complement(1909..3144)
                     /locus_tag="O0X37_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Ile)(2)-agmatinylcytidine synthase"
                     /protein_id="HJK61495.1"
                     /translation="MDDTDSPDGMCTTYLGAMLAENLRRAGYTVGEMRLVRLNPNVVW
                     KTRGNAAVCLEISGGTPEEVFDSACRLVEEFAEFGCENTNPGIVVVNERPNPAFYYQA
                     LQRFCTIEETIQRLDAIGALYRGYKCGRGLIGALAAVSSVLPDATYELLAYRSPERFG
                     TQRKIVAESFFTSAAATFPHTWDTVDPTAGTVVCVPHGKDPVLYGIRGESPEWVKTSA
                     KMVQSEPPILTQIWRTNQGTDAHLLDYDGEPQEGLSYRMDGVVAGNPVTTCGGHVHFT
                     FKPDASEPLLTVFAFEPTKEFRHIVRMLRSGDCLTLCGSWKKNSLHLEKFRINDLSDE
                     QIRASPKCPVCGGRMTSAGRGKGYKCRTCSGRIREVATVPRKISVGWYEVPPNARRHL
                     AKPVVRIHEPQHLQGGQNG"
     gene            3207..>3797
                     /locus_tag="O0X37_00785"
     CDS             3207..>3797
                     /locus_tag="O0X37_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJK61496.1"
                     /translation="MSNDRLLQNVVSILIMAGYNVSERCEIRPRSFDLMTSDGKHLLV
                     IKVVSQIDSVNEDIAWDLDKIARHLGAVPLIIGERARDAPLERGAIYLRYGINAVSSA
                     TLYDYLAEGELPLVYASPGGLYVNIDADRLRELREEHSMSLGDLAHALGVSRRTISKY
                     EGGMGTTLDVAMRLEELFNDDIVMPIDLLSYTPAAEE"
BASE COUNT          835 a          978 c          969 g         1015 t
ORIGIN      
        1 gtcatcagca tcgtctgcag ggtgaagttc tttggaacgc cgccgccgac cagaaccgca
       61 cccgcctttt tcaccgaaaa tgctgtattt aacagatcgt gcatgtcgcc gattgcatcc
      121 aggattgttt tcttgtggaa ctgggagtac atccagtact gcagaccaag aaccgagtcc
      181 tgaaacgctg ggcagtagat cggcacatcg tgttttgccg ctgttgcaag aattcccgtg
      241 tcaagttgct cgccgatggt gcgcagaagt ttactgatgg ttatcggctg ttccggaagg
      301 ctggagtaca catcctgaat aaacgactcc agttcttcat aggcttcgtt ggggaggaat
      361 acatcataga ttcggttaat ctcctccttt ctcagttcaa gatcatcaca ttccgcctgt
      421 ccgtgatagt ggtggcaacc gatcgcctca attaaatcat gtgtgaggtt tgcacctgtc
      481 gaagtgagaa tatcgatatg tcctttctcg attaatgtgg agacaatacc tcccattcct
      541 gcaggaacca tggctccgga cagaccgaag aacttcgtca tatccggatc ggacagcatt
      601 ttctcgtaaa tatcaaccgc atggtagagg ctgccgccat tgtatgctcc tgcacctccc
      661 atttccagaa cgagttcgtt caccgtcata cctgcgcggg gaatgaactg ttttaccgga
      721 tcattacagt gtttgtgcat ttattgtacc tgacctgaag taactaccat agagttaggc
      781 gagatacagc aataagatat tggaaaacag atttgactgt ggtgagggca gcagtatcca
      841 cactgctttg tactccacat cccccaccta actatactat tatgcgttag caacttttaa
      901 tcttgccgtc tctccccttg agaaaaccca gtttgcggag gtcgtttatg actcctgaga
      961 ggtagtctgc ggaaagatca aaggagatca catcctcggt ttcgccgagc gggaaggccg
     1021 gagcttcaaa ggcctcaagg agtcccgcct ctgttgtgat tcctcccgag acaaggtcat
     1081 gaatttttgc aataagtgcc tgtttgaaaa agattgcatc cacaaatctt cctgcttcgt
     1141 cgtcgtccac gtcaagatga tccagaacgt cactcatatc atcgatgccc tgtgcaagca
     1201 caatctccgc accggttgta actacataac cggtcgttga tgatgttctg atgtggctgg
     1261 tgatcccgga accgtcaagg tcttctggag caataccgcc gagatctccg taggcataca
     1321 taacgcgcag ttctttgggg tcctttacac cggtgagcat gtactcgggt gtttccccga
     1381 cgactttcag gagttcgtta tcctcaaggg ttccgagaat catcagggag ttgacaaatt
     1441 tctccgttgc ggtcttctgg atatcttcgt tgctgtccgc atcgatactt tccagctgtg
     1501 caaggagttg tgccggtgtt gcgtccgtta acacatcccc gactttgtgc gtggagaaaa
     1561 attcggcaag ggtttcttcg cggctcagga gatcggtttt gatctgggca aggtcgctgt
     1621 catcctcttt tgcaagaagt ttgctgatga gttcttcgaa tgcggcgatg gtcccttcga
     1681 agtacggggt cccggagaat gtatgctcaa ctgagatgcg ggcggaacag tccctctctt
     1741 tgagatactt ctggaactct gcagcatcct cccgcgagta aaatgtgatc ttctcctcaa
     1801 gagtcattgt tatgtatctt tttggtgcag gagagtgtta tcttttccgc cgcgtaatgt
     1861 gatgaaaaat ttctctggct ttttttgaaa aacaaagact ggaatttttt aaccgttttg
     1921 tccgccctgg agatgctgcg gctcatgtat tctgacaacc ggttttgcca gatgtcggcg
     1981 tgcattgggg ggaacttcat accagccaac tgaaattttc cgcgggactg tcgcaacctc
     2041 ccgtattctc ccggaacagg ttcggcattt gtatccttta ccgcgccctg cagatgtcat
     2101 acgtccccca cagactggac attttgggga cgcacggatc tgttcatctg agagatcgtt
     2161 tattctgaac ttttccagat gcagtgagtt ctttttccag ctgccgcaaa gcgtaaggca
     2221 atctcccgat cggagcattc gaacaatgtg ccggaactcc tttgttggtt cgaacgcaaa
     2281 gacggtaagc aacggttcgc ttgcgtctgg tttgaaggtg aaatgcacat gaccgccgca
     2341 tgttgttacc ggatttccgg caacaacacc gtccatacgg taggacagcc cttcctgcgg
     2401 ttctccgtca tagtcgagaa ggtgtgcatc ggtcccctgg ttcgtccgcc agatttgagt
     2461 caatatcggg ggctctgact gtaccatttt ggcggatgtt ttcacccatt ccggggattc
     2521 gccgcggatg ccgtaaagca caggatcttt tccgtgcggt acacagacaa ccgtgcctgc
     2581 cgtaggatct acggtgtccc aagtgtgcgg gaatgttgcg gcagctgatg tgaagaaact
     2641 ctccgcaaca attttcctct gtgtgccaaa ccgttcgggt gatcggtagg cgagcagttc
     2701 gtatgttgca tccggcagta cggatgatac ggccgcaagg gctccgatga gtccacgtcc
     2761 acatttgtat ccacggtaga gagcaccgat tgcatcgagc cgttgtatgg tttcttcaat
     2821 ggtacagaat ctctgcagcg cctggtagta gaatgccggg ttgggccgtt cgtttacgac
     2881 aacgattccc ggattcgtat tttcgcaccc aaactccgcg aactcctcga ccagtctgca
     2941 cgctgaatca aatacttcct ccggtgttcc gccggatatt tccagacaga ccgccgcatt
     3001 gccccgtgtc ttccacacca cattcgggtt cagacggacc agccgcatct ctcctacggt
     3061 atatcctgcg cgccgcagat tttccgcgag cattgcgccc agataggtgg tacacattcc
     3121 atctggggag tccgtgtcgt ccataccgag gaacatacta tcgatactat tttattgaac
     3181 tgcattacta ttacgattat acgatcatgt ccaacgaccg gctgcttcag aacgtggtca
     3241 gcatcctgat catggcaggc tacaatgtat cggaacgctg cgagatacgt ccgcggagtt
     3301 tcgatctgat gacatctgat gggaaacatc tgctcgtcat caaggtcgtg tcccagatag
     3361 acagtgtcaa cgaagacatt gcctgggacc tggacaaaat tgcccggcat cttggtgctg
     3421 ttccgttgat tatcggcgaa cgggcacgcg atgccccact ggagcgcggg gcaatttatc
     3481 tgcgctacgg aatcaatgcc gtgtcctcgg caacactgta tgactatctt gcggaaggag
     3541 agctgccgct cgtctatgcc tctccgggtg gtctctacgt gaacatcgat gctgaccggc
     3601 ttcgtgagct gcgcgaggaa cattccatgt cgctcggtga tcttgctcat gcactcgggg
     3661 tctcccgccg tactatcagc aagtatgagg ggggtatggg gacgacgctt gatgttgcaa
     3721 tgcggcttga ggagctgttc aatgatgata ttgtgatgcc gatcgatctt ctcagctaca
     3781 cgcctgcagc agaggaa
//
LOCUS       DALUAJ010000038         4245 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_7329,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000038 DALUAJ010000000
VERSION     DALUAJ010000038.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7329"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1890)
                     /locus_tag="O0X37_00790"
     CDS             complement(<1..1890)
                     /locus_tag="O0X37_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HJK61497.1"
                     /translation="MYAFTREESVSLEEYLETIPYVCNVSVNHKNGSILILYQKNCRA
                     DLLSVLTSIRIKNLPEPSVYGLETKRTDEAFVRDLTLLLFRRFVIRPLLPLPIRRIRT
                     IVRACSYFGRGGSSLLHGRLDTSVLDASAIGAAILQGDTKTASSIMFLLKLSGLLEDY
                     TKKRTRNALVHSLAINVDRVWVKNADGEMLIPISQVSQSDQVIVRTGTMIPVDGMISS
                     GDAMVNESSMTGEPLPVHKTTGATVYAGTVVEEGEITVAVRSLGGETRINKIVDLIDQ
                     SEALKADVQNKAEQLADSIVPYSFLGAAGIFLLTGNLTRALSVLMVDYSCALKLSTPI
                     SVISAMREASTMNIMVKGGKYLEEFALADTIVFDKTGTLTVASPKVVDVIPFAGYTKQ
                     DVLRTAACLEEHFPHSVARAIVRKAAEENLKHCEEHAAVEYVVAHGIATKLHGKRALI
                     GSAHFIFDDEGVPLGDEERAVIEKESEHGRSLIFLAIGDQLAGIICIEDAIRPMAEEV
                     ITSLKDLGITNVVMITGDGEATARAVASTIGIDRYYSQVLPEDKAAIINDLKASGRKV
                     IMIGDGVNDTPGLAAADVSVAMKDASDIAQEVADITLLSADLRELIHLRQLSTKLLDR
                     INTNYR"
     gene            complement(1952..2212)
                     /locus_tag="O0X37_00795"
     CDS             complement(1952..2212)
                     /locus_tag="O0X37_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61498.1"
                     /translation="MKDQRTWLFLGGVAVGAVAVTLLGSKTARDLAVAGLVQGMKMRD
                     DAEAALSSLKEDVEDAYVEKFQTPDDESGEEYTPVEVKKIEM"
     gene            2902..3621
                     /locus_tag="O0X37_00800"
     CDS             2902..3621
                     /locus_tag="O0X37_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJK61499.1"
                     /translation="MWCSLTNFPKTQLISSFQGFQEQIYILDNKMFRNYFQNVRKPNG
                     FGGRVMVSFMNRGHAAVSAWGFSHISPRKTDDVLDIGCGGGANLAVFLEKCSNGTVTG
                     IDYSPVSVEMSKKLNRRAVSSGKCMVLEGDVSALPFHEGMFDIVTAFETIYFWPDIEN
                     AFRQVLRVLKEGGIFLICTEVEGRGQKTEKWTKLIDGMNVYNGDDLICLLESAGFCEI
                     TVHRKQDRQWLCVLAKKPLNF"
     gene            3765..>4245
                     /locus_tag="O0X37_00805"
     CDS             3765..>4245
                     /locus_tag="O0X37_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61500.1"
                     /translation="MVDIIALIVVIAAFAIPLATGLVGAAPEGGSAAEYAEILGIWTL
                     IPPILALLLAFLTRNVILSLFLGVLSGAWMLALLGGDILGAIGGAFFGSTEYFISTLA
                     DRWNAGIIMQVLVIGALIALITRMGGMRAVAEAVAKRAKGPRTAQLATWIMGFVIFFD
                     "
BASE COUNT         1012 a          985 c         1020 g         1228 t
ORIGIN      
        1 ccggtagttt gtgttgatgc ggtccagaag ttttgtactc agctgtcgga gatggatcag
       61 ctcccgcaga tctgctgaga ggagggtgat atccgcaact tcctgggcga tgtctgatgc
      121 atccttcatt gcaaccgaca cgtcggctgc tgcaagtccg ggtgtatcat ttacaccgtc
      181 gccgatcatg atgaccttcc tgccggaagc tttcagatca ttgataattg cagctttgtc
      241 ttcggggagt acctgtgagt agtacctgtc gataccaatt gtggatgcaa ccgcacgggc
      301 ggttgcttcg ccgtcaccgg ttatcattac cacgttggtg atacccagat ctttcagaga
      361 tgtgatcacc tcttctgcca ttgggcgtat cgcgtcttca atgcagataa ttccggcgag
      421 ttgatcgccg atcgcaagaa agatcagcga tcttccgtgt tcactttcct tctcaattac
      481 tgctctctcc tcatctccaa ggggaacacc ctcatcatca aaaatgaagt gggcgctacc
      541 gatcaaagca cgttttccat gaagcttggt tgcaatgccg tgcgcaacca catactccac
      601 agctgcatgt tcctcacaat gcttgaggtt ttcctctgcg gcctttcgga caattgcccg
      661 tgcaacacta tgcggaaaat gttcctcaag gcatgctgct gtacgcaaaa catcctgttt
      721 ggtgtaaccg gcgaagggaa ttacatcgac aaccttcggg cttgcaacgg taagtgttcc
      781 ggttttgtca aacacaattg tgtcggcaag tgcaaactcc tccaggtact ttcctccctt
      841 taccatgatg ttcatcgtgg atgcctcacg catagcggag atgacagaga tcggtgtgga
      901 gagtttgaga gcacaggaat aatcaaccat caggaccgac agtgcccgcg tcaggttgcc
      961 ggtaaggaga aatatgcctg cagctccaag gaagctgtag ggaacaatcg agtccgcaag
     1021 ctgttctgct ttgttttgca catctgcttt cagtgcttcg gactgatcga taagatccac
     1081 aattttgtta atgcgtgttt ctccgccaag ggatctgact gcaactgtaa tctctccttc
     1141 ctctacaaca gtacctgcat atacagttgc accggttgtt ttgtggaccg ggaggggttc
     1201 accggtcatc gaggattcgt taaccattgc atctccagag gagatcatac catcaaccgg
     1261 aatcatggtt ccagttcgta caatcacctg atcgctttgg gaaacctggg agatggggat
     1321 taacatctct ccgtcagcgt ttttcaccca gacacggtca acattgattg caagactgtg
     1381 gacaagggca ttgcgggtgc gtttttttgt gtagtcctcc aagagtccgg aaagtttcag
     1441 aagaaacatg attgaacttg ctgtcttggt gtctccctgc aggatcgctg ccccgattgc
     1501 ggatgcgtcc agaaccgaag tgtcgagtct tccgtgcagg agacttgatc ccccgcgtcc
     1561 gaagtagctg catgcacgaa caatggttcg aatgcggcga atcggcagtg gaagcagggg
     1621 tcgaatcaca aaccgccgga acagcagtaa tgtaagatca cggacgaatg cttcatccgt
     1681 ccttttggtc tccagtccgt acactgacgg ctcaggcagg tttttaatcc ggatacttgt
     1741 aagaaccgaa agaagatcag ctctgcaatt tttctgatag aggatcagaa tactgccgtt
     1801 tttgtgattt accgagacat tacacacgta gggtatcgtt tccaaatact cttcaagaga
     1861 gacgctttcc tctctggtga atgcgtacat ccctcccctg atcctcatcc gtccgggaag
     1921 gtcatgaata atggtgtact gcatgagatc cttacatctc gatcttcttt acctcaaccg
     1981 gcgtgtactc ttcaccgctt tcatcgtcag gagtctggaa tttttcgacg tatgcatcct
     2041 ctacatcctc tttgagactg ctgagagcgg cttctgcatc atctctcatc ttcattccct
     2101 gaacaagtcc ggcaaccgca aggtcgcgtg cggttttact tccaagaagg gttactgcta
     2161 ctgctccgac ggcaacgccg ccaaggaaaa gccaggttct ctggtctttc atgatgtctt
     2221 gaatctgcac catgcctagt atttttcctg ttaggtgaac aaatagcgtc tccgaacttt
     2281 tttcggaata atggttattc cgaaggtgtt ttgtccttcc aaaacaggag ttgtatcttt
     2341 ttccaaataa tttttggaca gggcatttat cctcctaata tcttacgaat actctaggaa
     2401 catttgtgtt ccagtccctg cagacggtgc agaaaggagg ttctgataaa aaacgatgta
     2461 gcgaaaatcc tcagacatgc aatttcatgg attaaccatt ttgtccggca acatgggcga
     2521 tgttacccgc ctgccgtgtg tccacaatat tctctatcat cggcggagat accattcaaa
     2581 caaatggtca gatctgatct gtcctacctc aaatatccgc ctatcccctt ttggtttttg
     2641 ttatatttcc gattgatgtt cggactgtgt tttttaccac ctaacaggaa aatgatatgt
     2701 tgggacagtg cgatgtttat cccccaaaga aaaacactgc tgtttccaca aatatagcat
     2761 cagaaaagca ggcatcaccc gcataggtgc tgtactggta ttgtttgatg ctgtttgcac
     2821 ttgagttgtc tctatcaggc agcaggagag acacaacaag agaatttctg tcattggaga
     2881 ctgcattgaa tagagtcgga tttgtggtgc agtctcacca atttcccgaa aacccaactt
     2941 atttcttcat ttcagggttt tcaagaacag atttacatac tggataacaa aatgtttagg
     3001 aattattttc aaaatgtccg caaaccgaat ggttttggcg gaagagtaat ggttagtttt
     3061 atgaataggg gtcatgctgc tgtatcagcg tggggttttt ctcatatctc tccgcgtaaa
     3121 accgatgatg tacttgacat cggttgtgga ggtggcgcaa atctagctgt ttttctggaa
     3181 aaatgttcga atggaactgt gactggaata gattactctc cggtgagtgt tgagatgtcg
     3241 aaaaaactga accgacgtgc tgtatcttct gggaaatgca tggtactaga aggagatgtc
     3301 tctgcacttc cgtttcatga agggatgttt gatattgtta cagcgtttga gacgatctac
     3361 ttttggccgg atattgaaaa cgcattccgt caggttctgc gtgttctaaa ggagggaggt
     3421 atatttctga tctgcactga ggttgaaggt cgtgggcaaa agacagagaa gtggacaaag
     3481 cttattgatg gcatgaatgt ttataacgga gatgatctta tttgtctact ggagtcagca
     3541 ggattttgtg agattacagt gcatcggaag caggatcgcc agtggctctg tgttcttgcc
     3601 aaaaagcctt tgaattttta attcctctct ttcgtttaca gcatttttta tttactcatt
     3661 ctgccggata ttaatgagtc aaagctcata aataggggac cgtttaatat ctttctcatt
     3721 caggtaatat taccctgagg tgtgttctta tggaaaaatc aaagatggta gatatcatcg
     3781 ccctgattgt ggtaatcgcc gcctttgcaa taccacttgc aactggtctt gtcggtgccg
     3841 cacccgaggg gggctcagca gcagagtatg ctgagattct tggaatctgg acgctgatac
     3901 cgccaattct cgcactgctt ctggcattcc ttaccagaaa cgtgattctc tcactgttcc
     3961 ttggtgtact ttccggtgca tggatgcttg cccttcttgg aggcgacatt ctgggtgcca
     4021 ttggcggggc attcttcggt tctacggagt acttcatctc cacccttgcg gaccgctgga
     4081 atgcaggtat tattatgcag gtactcgtga ttggtgcact gattgctctc attactcgta
     4141 tgggaggcat gcgtgcagtt gccgaggctg ttgcaaagcg ggcaaaaggt ccgcgtaccg
     4201 cacaactcgc aacctggatc atgggatttg ttatcttctt cgacg
//
LOCUS       DALUAJ010000039         3759 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_7567,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000039 DALUAJ010000000
VERSION     DALUAJ010000039.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7567"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="O0X37_00810"
     CDS             complement(<1..1059)
                     /locus_tag="O0X37_00810"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HJK61501.1"
                     /translation="MGIVRELASVGHDLGAVFLLIGLATILPLGVGAYYQEWDAFSVM
                     GQTTLLYLILGGALRFLPKGDIKSRNSLSIAATALVWVFVSFLGCLPFLFTGMNLLDA
                     AFESMSAWTGTGFSLATNIEEWPKTLLLWRSFMQWIGGLGIIALTLTVVNKAGLVTRG
                     LYRSEGRSEAFMPSVIATAFQMWKIYVVLTVISLLAIMATGLNLWDAVNVAFCAISTG
                     GMSIYANGIMHFQNLGLEMVLIPIMLAGALPFRLYYLAYTNHSFKEILSDRVLHLLVT
                     VFLLVAAFLTCDLVLFNDYTLLDAVREGFFMAGTAISSTGFQNTTLAGWEMASILFLA
                     VFLLIGGAPGSTAGGIKLD"
     gene            complement(1153..1866)
                     /locus_tag="O0X37_00815"
     CDS             complement(1153..1866)
                     /locus_tag="O0X37_00815"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HJK61502.1"
                     /translation="MKQGDLLYLGKAKSVYLTENPDELLVVFRDDITAFDGQKKNVLS
                     GKGGYNAKVTAYFYPLLEAAGIPTHFLAAESANMHRVRKLAMIPLEVIVRNRAAGSLV
                     KNYGFTEGQPLSPPLIVMDLKDDSRHDPMVNDEIILALGLATAEELAEIKRLALAINA
                     VLKENLAAKDLDLIDMKFEFGRDAAGTIRLGDEISMDSLRMWDHGTIGASLDKDVYRK
                     ELGDVMETYAAVARRICGE"
     gene            complement(1913..2917)
                     /gene="cofG"
                     /locus_tag="O0X37_00820"
     CDS             complement(1913..2917)
                     /gene="cofG"
                     /locus_tag="O0X37_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase subunit CofG"
                     /protein_id="HJK61503.1"
                     /translation="MRSAPNLRTAMSRTITFSRNVFLPLTNVCANACGYCSFKAPVTD
                     GCVMPKTEVLATLRRGAECGCTEALFTFGERPENEPGFRRYIEAAGYSDILSYCEAMS
                     RAAIDLGMLPHTNAGILTYEELEHLRCVNASMGLMLETTAEIPAHRHSPGKDPAVRLE
                     MMENAGRLKIPFTTGLLLGIGETAADREESLAAIRDLHKRYGHIQEVIIQNFCPKEGT
                     EMADVPGASQEVIADTIRLAAEILPNDISIQIPPNLADAGKLLSLGVTDLGGVSPVTI
                     DYINPEHPWPALDALADIAAGYELRERLCVYPQYCNAEWISPELLPLVKRLAERVYSE
                     "
     gene            complement(2947..>3759)
                     /locus_tag="O0X37_00825"
     CDS             complement(2947..>3759)
                     /locus_tag="O0X37_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61504.1"
                     /translation="SPLWFYSPLLRHDDGTVRFSDIHALAVHEAETNDILMTTFGRYR
                     CCTINLALHPCETPEGVVDELREDWDHVALSLMNAENSKTMNQNYGLLFRQPGRLMTI
                     EPYQDGIPLAPKPVYTDRDKYIETLIFDPSNNTVSWMQDGICTADSVSEEALRECFRS
                     HSLPFNSPRLSDKDTFCFAALLEWGRQSAGKNRNEYALHIHTSTVGGLIFCTLNVLLH
                     PIDGTNAFDPVLDDWNQVKQIVHEADKKKTLAKNLPLLLTFSGYDAEGGRSL"
BASE COUNT          921 a         1021 c          974 g          843 t
ORIGIN      
        1 gtccagtttg atacctccgg cagtacttcc gggcgcaccg ccgatgagca agaacactgc
       61 caggaacaga atcgatgcca tttcccatcc cgcaagggtg gtgttctgga atccggtcga
      121 actgattgca gttcccgcca taaagaaccc ttcccggaca gcatcaagca gcgtgtagtc
      181 gttgaacagc accagatcac acgtaaggaa tgcagcaacc agaaggaata ccgtaacaag
      241 caggtgaaga acgcggtcgg aaagaatctc tttgaaggag tgatttgtgt aggcaagata
      301 atagagacgg aacggcaggg cacccgcaag catgatcgga atgagcacca tctccagtcc
      361 gagattctgg aagtgcataa ttccattcgc ataaatggac atccctcccg tggagattgc
      421 acagaacgca acattcaccg catcccacag attcaggccg gttgccatga tggcaagaag
      481 cgagatgacc gtaagaacga catagatctt ccacatctga aaggcggttg cgatcacact
      541 tggcataaac gcttccgacc gtccctccga ccggtagagg ccgcgggtga caagacctgc
      601 tttgttgacc acagtaaggg taagtgcaat gattcccagt cctccgatcc actgcataaa
      661 ggaacgccac aaaagcagtg ttttgggcca ctcctcaatg tttgtagcaa gcgagaatcc
      721 cgttcccgtc catgccgaca tcgactcaaa cgccgcatcc agcagattca ttccggtgaa
      781 caaaaacggc agacacccaa gaaaactgac aaaaacccag acaagtgctg ttgctgcaat
      841 ggacaaactg tttcgcgact tgatatcccc tttgggaaga aaacgcaggg caccgccgag
      901 aatgaggtac aggagtgttg tctgtcccat cacagagaat gcgtcccact cctgataata
      961 tgccccgacc ccgagcggca gaatggttgc aaggccgatc aggagaaaaa ccgctcccag
     1021 atcatgtccg acacttgcaa gctcacgaac gatgcccatt tgatatatga tgatcgaacc
     1081 tgctcaaata taatgatacg ggatcgattg tctgcatcac ccgaatttgt ttacaaaaaa
     1141 gaaaaaatga aattattcgc cgcagatgcg ccgggcaacc gctgcatagg tctccatgac
     1201 gtcgccaagt tccttgcggt acacgtcctt gtccagcgat gcaccaatgg taccgtgatc
     1261 ccacatccgc agggagtcca tgctgatctc atcacccagc cggatcgttc cggcagcatc
     1321 cctgccgaac tcgaacttca tgtcgatcag gtccaggtct tttgctgcaa gattctcctt
     1381 tagtactgca ttgatggcga gtgccagccg tttgatctct gcaagttctt ctgcggtggc
     1441 aaggcccagt gccaggatga tctcatcgtt gaccattgga tcatgccgag agtcgtcttt
     1501 gagatccatt acaatcaggg ggggagataa cggctgcccc tcggtaaatc cgtagttttt
     1561 caccagcgat cccgcggcac ggttgcggac aattacctcc agcgggatca ttgcaagttt
     1621 tcggacccgg tgcatgttcg ctgattcggc tgcaagaaaa tgcgtcggga ttccggcagc
     1681 ctccagaagg ggatagaagt atgccgtaac ctttgcattg tacccgccct tgccggaaag
     1741 gacgttcttc ttctgtccgt caaacgcggt aatatcatcc cggaacacta ccagaagctc
     1801 gtctgggttc tcggtcagat acacggactt tgccttaccc aaatagagaa gatcgccctg
     1861 tttcatgatg ccagatctat tgcacggcaa ccatgataag tgttagcatg aatcactcgg
     1921 aataaacccg ttctgcgagg cgttttacga gcgggagaag ttccggcgaa atccactcag
     1981 cgttgcagta ctggggatac acacacagcc gttcccgcag ttcgtatcct gcggcgatgt
     2041 ctgcaagggc gtccagtgcc ggccaggggt gttccgggtt gatgtagtcg atggtaaccg
     2101 gcgaaacacc gccaagatcg gtgactccga gggagagcag ttttcctgcg tccgcaaggt
     2161 tcggcggtat ctgaatcgag atatcgtttg gcagaatctc ggctgcaagc ctaatggtgt
     2221 cggcaatgac ttcctgtgaa gcacccggaa cgtccgccat ctcggttccc tcttttggac
     2281 agaagttctg gatgatcacc tcctgaatgt ggccgtagcg tttgtgcagg tcacggattg
     2341 ccgcaagcga ctcctcacgg tcggccgccg tctcgccaat gccgagcaga agcccggtcg
     2401 taaacggaat cttcagccga cctgcgtttt ccatcatctc aaggcggact gccggatcct
     2461 ttcccggact gtggcggtgt gccggaatct ctgcggttgt ctccagcatc agtcccatgc
     2521 tcgcgtttac gcaccggaga tgctccagct cctcgtacgt taaaattcct gcattggtgt
     2581 gcggcagcat tccaagatca atcgctgccc ggctcattgc ttcgcagtag gaaaggatgt
     2641 cagaatatcc tgccgcttcg atataccgcc gaaatcccgg ttcgttctcc ggccgctctc
     2701 caaaagtgaa cagtgcctcg gtacagccgc actcggcacc gcggcgcagt gtggcgagaa
     2761 cctccgtctt tggcattaca cagccgtcgg tgacgggtgc cttaaacgaa cagtatccgc
     2821 aggcatttgc acacacattt gtcagaggaa ggaatacatt ccgggaaaac gtaatcgtcc
     2881 gggacatagc tgttctaaga ttgggtgctg aacgcatatg aacctgtccg gtttcccaaa
     2941 aaaagattag agggaacgtc cgccttcggc gtcgtacccg gaaaaggtca gaagcaaggg
     3001 gagattcttt gcaagcgttt tctttttgtc cgcttcatgc acaatctgtt tcacctgatt
     3061 ccagtcatcc agaaccggat caaatgcatt cgtcccgtct atgggatgaa gaagtacatt
     3121 cagcgtgcag aagataaggc cccccacagt tgacgtatga atatgcagcg catactcatt
     3181 cctgtttttt cccgccgact gcctgcccca ttcaagcagt gccgcaaaac aaaaagtatc
     3241 tttgtcggag agacgcggag agttgaacgg cagtgaatga ctgcggaagc actcccgcag
     3301 tgcttcctcg gagacggaat ctgcggtaca gatgccgtcc tgcatccatg agacggtgtt
     3361 gttcgacgga tcaaatatca gtgtttcaat gtacttgtcg cgatcggtgt aaaccggctt
     3421 tggtgcaagg ggaatgccgt cctgataggg ttcaatggtc atcagtctgc cgggctgccg
     3481 aaacagcaaa ccatagttct ggttcatggt tttgctgttc tcggcattca tgagtgacag
     3541 agcaacatga tcccaatctt cccggagttc atcaacgacc ccctccggcg tttcacacgg
     3601 atgcagggcg agattgatcg tacagcaccg gtatctgccg aaagttgtca tgagtatatc
     3661 gttcgtttcg gcttcatgca ccgccaatgc gtgaatatcc gaaaaacgga cagtcccgtc
     3721 atcatgccgg agaagcggtg agtagaacca cagtggaga
//
LOCUS       DALUAJ010000040         3397 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_7727,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000040 DALUAJ010000000
VERSION     DALUAJ010000040.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7727"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="O0X37_00830"
     CDS             complement(<1..1179)
                     /locus_tag="O0X37_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HJK61505.1"
                     /translation="MNPNELVQFLKKNPADFTKDDIIRFCEENGIEMVNFRYVAGDGK
                     LKTLNFVISSKEYLDTILSDGERVDGSNIFPFIEAGSSDLYVVPRYGTAFVDPFAEVP
                     TLDILCSYYDNEGKPLASSPEYILRCARDAFKKNTGMDFKCLGELEYYVICPEEDLYP
                     GLDQKGYHASGPFCKFEEMRTEAMLMVARAGGQIKYGHSEVGCFTKDGYYYEQHEIEF
                     LPTDPILAVEQIVIAKWVLRMLGYKYGVEVSYAPKITVGKAGSGLHFHMLAEKNGKNM
                     MVKDGQLSDIAKRMIAGILDVGDAVTAFGNPIPTSYLRLVPHQEAPTYICWGDRNRSV
                     VVRVPLGWTGSTDMAAEANFGIRRKVNKASKQTFEYRVTDGSADIYETVSCLIIGAMH
                     G"
     gene            complement(1295..2272)
                     /gene="moaA"
                     /locus_tag="O0X37_00835"
     CDS             complement(1295..2272)
                     /gene="moaA"
                     /locus_tag="O0X37_00835"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HJK61506.1"
                     /translation="MTTGKKPVNEILTDPYGRTITNVRIALTNACNLRCIYCHREGEE
                     INGCSRNDTHIQMTKEEIAELIGVFAELGVTTIKLTGGEPTLRSDLLDIIRSIPPHIE
                     SSMTTNGTLLAKMATDLKEAGLSRVNISLDTLHRDRYLKITGRDLLPDVLAGIDAAIA
                     AGLTPVKLNMVILKGINDDEIEDFFAYVRGRKDLILQIIELMDINGWADNIDHVDDLI
                     RGNADLVGDLEKDIASRSTKVTTRRMHHRRKYCLDNAEVEVVRPMHNLEFCANCNRLR
                     VTSDGKLKPCLLRTGNEVDIRGLHGEDLKAAIAKAVQNRAPYFTPAPKV"
     gene            complement(2244..2858)
                     /locus_tag="O0X37_00840"
     CDS             complement(2244..2858)
                     /locus_tag="O0X37_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HJK61507.1"
                     /translation="MGSQWTKDNIYRKSVRDGYRARSAYKLIDINQRFNIIRKTDNVV
                     DLGAAPGSWLQVLKTMTDGQLLGVDLNPIVPMENVITLTGDFTTPEIQEKVRELMPLV
                     NVVVCDAAPHLSGAKAYDQARVMALNEEALRFAEKLLKQGGNFVMKSFQGTDFNELLE
                     LVKERFYSVRVIRSTATRRGSTECYIVAKNFIGDADDDRKKTSE"
     gene            3173..>3397
                     /locus_tag="O0X37_00845"
     CDS             3173..>3397
                     /locus_tag="O0X37_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HJK61508.1"
                     /translation="MKLVYTGKTKNVFALDDGNYLLKFKDDCTGADGVFDPGMNTVGL
                     TIDGAGRAGLRLTKHFFEILNKKGIPTHYID"
BASE COUNT          780 a          835 c          860 g          922 t
ORIGIN      
        1 accatgcatt gccccgataa tcaggcagga aacggtctcg tagatgtcag ccgatccgtc
       61 agtcacgcgg tactcgaacg tctgtttgga tgctttgttt accttgcggc ggatgccaaa
      121 gtttgcctcc gctgccatgt ctgtagaacc tgtccatccc agcggaacac ggacaacaac
      181 tgaccggttc cgatctcccc agcagatgta agtcggggct tcctggtgcg gaaccagccg
      241 caggtaagag gtcggaatcg ggttgccgaa tgcggtaacc gcatcaccga cgtccagaat
      301 acctgcaatc attctctttg ctatgtcgga aagctggccg tcttttacca tcatgttctt
      361 gccgttcttt tctgcaagca tgtggaagtg cagacctgat cctgccttgc cgacggtgat
      421 cttcggtgca taggaaacct cgacgccgta cttgtagcca agcatccgca gtacccattt
      481 tgcgatcaca atctgctcaa cggcaagaat tggatcggtt ggcaggaact caatctcgtg
      541 ctgctcgtag tagtagccgt ccttggtgaa gcagcctact tcagagtgac cgtacttgat
      601 ctgtcccccg gcgcgtgcaa ccatcagcat agcttcggta cgcatctcct caaatttgca
      661 gaacggaccg gatgcatgat agcctttctg atcaaggcca ggatacagat cctcctcggg
      721 acagattaca taatactcaa gttcacccag acatttgaag tccatgccgg tgtttttctt
      781 gaacgcatcg cgcgcacagc gcaggatgta ctctggagag gatgcaagag gcttaccttc
      841 attgtcatag taggaacaga gaatgtcaag cgtcggcacc tctgcaaacg ggtctacgaa
      901 tgctgtgcca tagcgcggta caacatacag atcggaactg ccggcctcga tgaacgggaa
      961 gatgttactg ccgtcaacgc gctcgccgtc cgagaggata gtgtctaagt attctttgga
     1021 agagattaca aaattcagtg tcttgagttt tccatcgcct gcaacgtagc ggaagttcac
     1081 catctcgatt ccgttttcct cgcagaaccg gatgatatcg tccttcgtaa aatcagcagg
     1141 gtttttcttc aggaactgta ccagttcgtt aggattcatc agtacttctg aatcattcat
     1201 tgccgtaact attcgaaggg acggcataaa tacccaacca tgcgattgtg tgacatgatg
     1261 tatatcaaag gaaaaaaggt acttggcaga agtttcagac tttgggtgca ggagtgaagt
     1321 aaggagcacg gttctgtact gccttggcaa ttgctgcctt taagtcctct ccgtgcagtc
     1381 cccggatatc cacttcattt ccggtacgga gaaggcatgg cttgagttta ccgtccgagg
     1441 ttacccggag gcggttgcag tttgcacaga actcaagatt gtgcattggc cgtacgactt
     1501 ctacctcggc gttgtccaga cagtattttc tacggtgatg catcctgcgg gtggtgactt
     1561 tggttgaacg tgaggcgatg tccttttcca aatctccaac aagatctgca tttccacgga
     1621 taagatcgtc gacgtgatcg atgttgtccg cccagccgtt gatgtccatg agttcgatta
     1681 tctggaggat gagatccttt cttccacgga cgtaagcgaa gaaatcctca atttcatcat
     1741 cgttgattcc cttgagtatt accatattta gtttgaccgg agtcaggcct gcagcgattg
     1801 ccgcatcaat ccctgcaaga acatctggca ggagatcacg gccggtgatc ttcaggtatc
     1861 ggtcccggtg cagcgtatca aggctgatgt tgacacggga aagccctgcc tccttgagat
     1921 ctgttgccat ctttgcaagc agtgttccgt tggtggtcat cgaggattcg atatgtgggg
     1981 gaatggaacg aataatatca aggagatcag atcgaagtgt gggttccccg ccggtgagtt
     2041 ttatagtggt tacaccaagc tctgcaaaga caccgatcag ttcggcgatc tcctctttcg
     2101 tcatctggat gtgggtatcg tttcgtgagc agccgttgat ctcttcacct tcgcggtggc
     2161 agtagataca ccggaggtta caggcgttgg tcagggcaat cctcacatta gtgatagttc
     2221 ggccgtaagg atcggtaaga atctcattca ctggtttttt tccggtcgtc atcagcgtct
     2281 ccgataaagt tcttagccac aatataacac tcggtgcttc cccggcgggt ggcagtggat
     2341 cggatcaccc tgacggagta gaaacgttct ttgacaagtt caaggagttc gttgaaatca
     2401 gttccctgga aggatttcat aacaaagttg ccgccctgtt tcaggagctt ctcggcaaac
     2461 cgcagtgctt cctcatttag tgccatcacc cgtgcctgat catacgcctt tgccccggag
     2521 aggtgtggtg cggcatcaca gacaacaacg ttgacaagcg gcatgagttc ccggactttt
     2581 tcctgaattt ccggtgttgt gaagtcgcct gtcagggtta tgacattttc catgggaaca
     2641 attgggttga gatctacacc gagcaactgg ccgtcggtca tagttttgag tacctgcagc
     2701 cagcttcctg gagcagctcc gagatcaacc acgttgtcgg ttttacggat tatattgaac
     2761 cgctgattga tatcgataag tttgtaggcg gaacgggcac ggtatccgtc acgcacggat
     2821 tttcgataga tattgtcttt tgtccactgc gatcccatta cggctgtctc cgttttagta
     2881 ttattatagc gcggtgtggt gatataatgt agccggttct tgcaacccgt gtgattttcg
     2941 ggaaaaggtt ctgtggattt tacttatgta atactcattt ttctaaaaac agtcattcag
     3001 tgggtattat tgttaggagg ggtctgttct attttgcatg cgatattttc tttcgcgttg
     3061 gctgttgtgt tcctgtacat taagatcgcc ctccttcttt tgttccattt gcacggacca
     3121 gatggttatg ccgccaaaga attatacccg aatacggaca acatttccta ccatgaaact
     3181 ggtatacacc ggcaaaacaa agaacgtttt cgcacttgat gacggaaact atctgctgaa
     3241 gtttaaggac gactgcaccg gtgccgatgg agtcttcgac ccgggcatga acacagttgg
     3301 cctcaccatt gacggcgcgg gccgtgccgg tctccggttg acaaaacact tctttgagat
     3361 cctcaacaag aagggcattc caactcacta catcgac
//
LOCUS       DALUAJ010000041         1988 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_7848,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000041 DALUAJ010000000
VERSION     DALUAJ010000041.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7848"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1004)
                     /locus_tag="O0X37_00850"
     CDS             complement(<1..1004)
                     /locus_tag="O0X37_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832677.1"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HJK61509.1"
                     /translation="MVFRVFVEKKPALANESHALLRELSTLPGVQGVSSLRIINRYDV
                     DNIDADLFAYAKSTVFSEPQIDIVTDTPALAEDERMFAVEYLPGQFDQRADSAAQCIQ
                     LISQKERPIVRTARVYVLGGNISDEVLDRVKKYVINPIESREADLAMPTTLVMSCAAP
                     AKVAVLEGFRELDAAGLADFSARYGLAMDTADLAFCQKYFLNENRDPTITEIRMIDTY
                     WSDHCRHTTFLTTIDSVRTESPLLQKAFDDYLAVRQSIGRTKPVNLMDIGTIAVKYLR
                     SQGKLGKLDESEEVNACTVKITVSVDGKDEDWLLLFKNETHNHPTEIEPFGGAATCIG
                     G"
     gene            complement(1119..1481)
                     /locus_tag="O0X37_00855"
     CDS             complement(1119..1481)
                     /locus_tag="O0X37_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HJK61510.1"
                     /translation="MPEDPSECPKGRCGRPRVKRCIDLDGASGCYAPICPHRQQTVTS
                     VVVFPEEIAVLKLVDLDNLSQEEAAGMLGVSRKTAWRDLHEARRKIADALVNGKAIRV
                     SCCPHENMCSSLSPDREK"
     gene            1655..>1988
                     /locus_tag="O0X37_00860"
     CDS             1655..>1988
                     /locus_tag="O0X37_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJK61511.1"
                     /translation="MTTPFITIENLCIDFPADADNPAGEQVRILNDINLEIAEGEIVG
                     VIGRSGCGKTVLIHLLRGIEQPPTAGKIIYHISRCPACGRIEFRSSAGKKCPACGAVL
                     EAQDVDFWA"
BASE COUNT          522 a          495 c          491 g          480 t
ORIGIN      
        1 ccgccgatac aggttgctgc ccctccaaac ggctcgatct cagtaggatg attgtgtgtc
       61 tcgtttttga acaaaagcag ccagtcctca tctttcccat caacagagac agtaatcttc
      121 acagtacagg cgttgacctc ctcggactca tcgagcttgc caagcttacc ctggctccgc
      181 agatatttga cagcaattgt tccaatatcc atcaggttga caggttttgt tctgccgata
      241 gactgccgga ctgcaagata atcgtcaaag gctttctgca ggaggggact ttcggtccgg
      301 acactatcaa tagtggtaag gaacgttgtg tggcggcaat gatccgacca gtaggtatca
      361 atcatcctaa tctcggtaat agtcggatca cgattctcat taaggaagta cttctgacag
      421 aaggcgagat ctgctgtgtc catggcaaga ccgtaacgtg cggaaaagtc ggcgagacct
      481 gcggcatcaa gctcacggaa tccctcaagc acagcaacct ttgcaggagc agcacaactc
      541 atcacaagag ttgtcggcat cgcaagatcg gcttcacggc tttcgatcgg gttaataaca
      601 tattttttga ccctatcaag cacctcatcc gagatgtttc cgccaagaac atagacgcgg
      661 gccgtacgga caatgggacg ttctttctgg gaaataagct gaatacactg tgcagcactg
      721 tcagcacgct gatcaaactg gcccggcaga tactcaacgg caaacatccg ctcatcctct
      781 gcgagagcgg gagtgtctgt gacaatatca atctgcggct cagaaaacac agtgctcttt
      841 gcatacgcaa aaaggtcggc atcgatgtta tcgacatcgt agcggttgat gattcggagc
      901 gaggaaactc cctgcactcc cggcagagtg gaaagctcac ggaggagcgc atgcgattca
      961 tttgcaagag caggtttctt ctcgacaaat actctgaaaa ccattgtaaa atccctacct
     1021 gtcagcccgg ttagctgtat agtatatagg aggaatgggt tttttagttt gctgtactgg
     1081 gagaggaaca aattacaaaa aagtacaaag taaaaagatc acttctcgcg gtccggggag
     1141 aggctgctgc acatgttctc atgcgggcaa caggagacac ggatggcttt accgttcacc
     1201 agtgcatcag caatctttct ccgcgcttca tgaagatcac gccatgcggt tttgcgggac
     1261 acaccaagca tacccgctgc ctcctcctgg gagagattat ccagatcaac aagcttcagg
     1321 accgcaatct cttcaggaaa cacaacaacc gatgtcacgg tttgctgacg gtggggacag
     1381 attggggcat aacaaccgga ggcaccatca agatcgatgc accgtttcac cctcggtcgg
     1441 ccgcatcttc cttttggaca ttcactggga tcttccggca tgctatcttt cctgtatcat
     1501 ttttttctct gtcgcgtaaa agtagttttg tccgtcaaca aagactgctt aaattactca
     1561 tatgcgccaa agtatataat agaccctgat aaaatactat gagagtattt tccggcagcc
     1621 gaaactgcct gccggtattc gaggtagtgt gcaaatgacg acgccattta tcaccattga
     1681 aaatctctgc atcgattttc ccgcagatgc agacaatcca gcaggcgagc aggtcaggat
     1741 tcttaatgat atcaatctgg aaatagctga aggagagatt gtcggtgtga tcggcagatc
     1801 cggctgcggg aaaacggttc tgattcacct gttgagagga attgaacagc cgcctaccgc
     1861 aggaaagatc atctatcata tttcccgctg ccccgcatgt ggcaggattg agttccgtag
     1921 cagtgcaggc aagaaatgtc cggcctgtgg tgcagtgctt gaagcacagg atgtggactt
     1981 ctgggcac
//
LOCUS       DALUAJ010000042         5818 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_7878,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000042 DALUAJ010000000
VERSION     DALUAJ010000042.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7878"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="O0X37_00865"
     CDS             <1..692
                     /locus_tag="O0X37_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HJK61512.1"
                     /translation="VMTPRTIPVHDLVRCSICPLQVYLARSDPEEFQEPVNYSIAKQI
                     SLHFGDELKPDEIWDELETVLSVAGAEEYEQTLAMIDACQKTVWRPAVDTDVFVSSQK
                     YGISGKVDRLFDDGFAIVRSSKAPSSGIFAADRLRLTAYWLCLYEEHKRSFDCSVEYL
                     GSGTVRNLASPTPADRRAFLAALHSAESVFQGEVPRPRQGHHCLSCIFHERCDTVLRP
                     KSLFERLNLVR"
     gene            749..1399
                     /locus_tag="O0X37_00870"
     CDS             749..1399
                     /locus_tag="O0X37_00870"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HJK61513.1"
                     /translation="MTSVLSEDVLEKIFNTGTDEVPLHHLTFPHHSESEIENVLNILA
                     ERGFVCRGNNTVRLTGKGKEKAAQVARKHAVLETFLKDVLGKQPDAASKEACILEHNI
                     SSETIERLDNFLDQRKPEPVPSETTGFINHNNLTRRNPIVPLSECPEGSLLHVSMIRC
                     FAKHNRLIDLGVIPGELITLRRKLVNNAVVITVKGCDIALSPEVSANIMVERCETP"
     gene            1396..3264
                     /gene="feoB"
                     /locus_tag="O0X37_00875"
     CDS             1396..3264
                     /gene="feoB"
                     /locus_tag="O0X37_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HJK61514.1"
                     /translation="MMPRAVLIGNPSVGKSLIFNQLTGLGVEVSNYPGTTVGVMHGVV
                     RYDDAEFSLVDLPGIYSLSGDSIEEKMVREYLLAEELDLLIAVLDAKHLERNLYLLLQ
                     VAEIKKPLLVILNMMDEAEAAGKIIDTVDLSRKLGVPVIGSVATSGKNLDQIARFVLA
                     NDVPVPTLVVPYDHHIEAAVHSLQKYYGISQSEALWALENVSVSTITPEIHENALVIA
                     EEIERRHMMSPEQIIAANRHNLAKQLAASVTSTAPPRRRRDFDALLTRGFPGLPILAL
                     IMVSILLIVFTLGGILEETIVSLMTTYISDPFHALDLDPFVDTLGGAVILALMSGRGI
                     ACPYVFLFYLFISVLEDTGYLTRAAFLADRGMHKLGLHGQGLIPMVLSFGCSVPAIMS
                     TRLLPTRRERVIASVLVTMLPCSARTVVISGIVASFIGFGAALSIYAIVFVLVFVVGC
                     ILSRITAGEQYGMILEMAELRRPIASHVVRKAWMRVREFIYIAMPLLLVSSIFLGLFE
                     YYGLVAMFEEFIDPISTAVLGLPGFAFTALMFGILRKEMAFETLAVMAGTTDLAAVLT
                     SGQLYIFALVCALFIPCISTIAVLMRETGVRYAAMITVFTLCLGIGLGALTHLILL"
     gene            3308..4066
                     /gene="dph5"
                     /locus_tag="O0X37_00880"
     CDS             3308..4066
                     /gene="dph5"
                     /locus_tag="O0X37_00880"
                     /EC_number="2.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004164 - diphthine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine synthase"
                     /protein_id="HJK61515.1"
                     /translation="MLTFIGLGLCDEYDVSIRGLEAIRQADAVFLEVYTSVLTGTTIP
                     RLEEYYGKKITPLYREDVELHPEPILDAAANGDAVFLTAGDSMVATTHADIRIRAAER
                     GIPTRIIHGASITTAVCGLSGLQNYRFGKSVSVPFPYGKWFPMTPIEVISANLSQNLH
                     TLVFLDIQKDKERYMRIAEAVDLLEEQASRAGTSIPLYIGIARAGSENPVVHAGDAND
                     MREFEFGAPLHIMVVPAELHDVEREYLERFAGLC"
     gene            4060..4629
                     /locus_tag="O0X37_00885"
     CDS             4060..4629
                     /locus_tag="O0X37_00885"
                     /inference="COORDINATES: protein motif:HMM:NF015943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF357 domain-containing protein"
                     /protein_id="HJK61516.1"
                     /translation="MLIESYGKEFTTDSSAACPVAPASTPLGATAAEILEMAYCYASD
                     GMVFYRKGDLVNAVASFAYGYGWLDAGVYLGYVNGRPATSLPVISEQIPDTLFDHLSE
                     KTSRYQRMLSSALDGVAVLPDTETRAFSAAQNIRTTAEVNYAEGTTLVLAKDLTNALV
                     SFSYGYGWLDVGVRTGLFGITGDRHLFTI"
     gene            4753..>5818
                     /locus_tag="O0X37_00890"
     CDS             4753..>5818
                     /locus_tag="O0X37_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2193 family protein"
                     /protein_id="HJK61517.1"
                     /translation="MSDIYKKMVEEALAAQYADVNVLKEKRGTRFSLSDAKPYVDAVE
                     KMTVADGQSAAVINLHKASVKTHFEVLSGLTGYVRPEDDPFVEHYQTPAILEILCDED
                     KKFAKSLETFAESIKKNENIIGREAARCYAGFYGPSCVVDFALMPGSTSNVVNQILQK
                     TDIPVDHKQAILAAKSWGMNTSYGIGDLFAKRIEAGDTLAEASDKEVKQLQALYQHPI
                     DAQVELMQNSGMSSFDPRKYMDNYRKGMESFVKAAIDDGVHYGNIATVPAYCVGDISH
                     HISQSTYNMCKDDVVMATIEAVTGVIDKTLHAALPEAKSGYQLLDVATGASAAAAEYL
                     LELDGFNAPMIVDLLTKRYHN"
BASE COUNT         1350 a         1343 c         1477 g         1648 t
ORIGIN      
        1 cggttatgac gcccagaacg atcccagtac acgatcttgt ccgttgcagt atctgtccat
       61 tacaggtgta tttggcccgt tctgatccgg aagagttcca ggagccggta aactacagca
      121 tcgcaaaaca gatatcccta catttcgggg atgaactgaa gcctgatgag atatgggatg
      181 aacttgaaac cgttttgtct gttgcaggag ctgaagagta tgaacagact ttggcaatga
      241 ttgatgcatg ccaaaaaact gtttggcgtc cagccgttga cactgatgtc tttgtgtcct
      301 cacagaagta tggtatttcc ggaaaagtgg ataggctgtt tgatgatggt tttgcaatcg
      361 ttcggagtag taaagcaccc agctccggta ttttcgcagc ggaccgactc cgtctgactg
      421 catactggct ttgtctctat gaggagcaca aaagatcatt tgattgttcc gtggagtatc
      481 ttggttctgg aactgtacgg aatcttgcgt ctcctacccc cgctgatcgc cgtgcgtttc
      541 ttgcagcact tcacagtgct gaatctgttt ttcaaggtga agtaccgcgt ccccgtcaag
      601 ggcaccactg tctttcctgt atatttcatg aacggtgtga cacagtttta cgcccgaaat
      661 ctctgtttga gcgtcttaat ctcgtaagat gacgatatgt agatatttgt tcagaacaga
      721 aatggtaggt gcaattgtgt gattctgcat gacctcggta ctctctgaag acgttttgga
      781 aaaaatattc aatactggca ctgatgaggt gcctcttcat catcttactt ttccccatca
      841 ctctgagtct gagattgaaa atgtcctgaa catccttgct gaacgaggtt ttgtgtgccg
      901 cgggaacaac acggtcaggc ttactgggaa aggaaaggaa aaagctgcgc aggttgcacg
      961 caagcacgca gttcttgaaa cctttctgaa ggatgttttg ggaaaacaac cggatgctgc
     1021 gtctaaagaa gcctgtattc tggaacacaa tatctcatca gaaacaatcg aacggctcga
     1081 caatttcctg gatcaacgta aaccggaacc agttccttcg gagacgaccg ggttcatcaa
     1141 tcacaataat cttacccgca gaaatccgat tgttccgctt tccgaatgtc cggagggttc
     1201 tcttctgcac gtttccatga ttaggtgttt tgcaaaacac aacagactaa ttgatcttgg
     1261 tgtgattcct ggtgagctga ttaccctgcg gcgtaaactt gtcaacaacg ctgtggttat
     1321 cactgtgaaa ggctgtgata ttgcgttaag cccggaggta tccgccaaca ttatggttga
     1381 acggtgtgaa acaccatgat gccgcgtgca gtacttattg ggaacccgag tgttggaaaa
     1441 tcgcttatct ttaatcaact gaccggtctt ggcgttgagg tgagtaacta tccgggaaca
     1501 acagttgggg tcatgcacgg tgttgtccgg tatgatgatg ccgagttctc ccttgttgat
     1561 ctccccggta tttactctct gtcaggtgac tctatcgagg agaagatggt ccgcgagtac
     1621 ctgctggcag aagaactgga tcttctgatt gctgtactgg atgcaaaaca ccttgagcgc
     1681 aatctctatc ttctcttgca ggttgccgaa atcaaaaagc cgcttcttgt aattcttaac
     1741 atgatggacg aggctgaggc tgccggaaag atcattgaca ccgttgatct ttcccggaaa
     1801 cttggtgtcc cggtcattgg ttctgttgca acaagcggta agaatctgga tcagattgcc
     1861 cgatttgttc ttgcgaatga tgttccagtc ccaacccttg ttgtacccta tgatcaccac
     1921 atcgaagcgg cagttcacag tctccaaaaa tactacggca tcagtcagtc tgaggcgctg
     1981 tgggcattgg aaaacgtttc agtgagcaca atcacgccag aaattcatga gaatgctctg
     2041 gtgattgcag aggaaattga gcgcaggcac atgatgtctc cggagcagat tatcgctgca
     2101 aaccggcata acctcgcaaa gcagcttgcc gcgtctgtta ctagtactgc tcccccgcgc
     2161 cgcagacgtg attttgacgc cctccttaca cgcggttttc cgggactgcc aatccttgcc
     2221 ctcatcatgg tctcgattct tctgatcgtg tttactcttg gaggcatttt ggaggagaca
     2281 atcgtttctt tgatgacaac gtatatctct gatccgttcc acgctcttga tctggatccg
     2341 tttgtggata cgcttggtgg tgcagttatt cttgccctga tgtctggtcg tggcattgct
     2401 tgtccatatg tatttctctt ctatctgttc atctcggtac tggaggatac tggatattta
     2461 accagggcag catttctggc agatcggggt atgcacaaac ttggtctcca tggtcagggc
     2521 ctgattccca tggttctgtc ctttgggtgt agtgtacctg ctatcatgag tacgcggctg
     2581 cttccgaccc gtcgtgagcg agtgattgca tcagtactgg ttaccatgct cccatgttcg
     2641 gcacggactg ttgtcatctc cggaatagtt gcatctttta tcggatttgg cgcggcgctt
     2701 tccatttatg cgatcgtgtt tgttcttgtc tttgttgtag ggtgtattct ctctcggatt
     2761 accgcgggtg agcagtatgg aatgattctt gagatggctg aacttcgccg tcccattgct
     2821 tctcatgttg tacgcaaagc gtggatgcgg gtgcgtgagt tcatctatat tgcaatgccg
     2881 ctcctgctgg tgagcagtat tttcctcggt ctctttgaat attacggcct tgttgcaatg
     2941 tttgaggagt tcatcgatcc gatttcaaca gcggttcttg gtcttccggg atttgcgttt
     3001 actgcactga tgtttggtat ccttcgcaaa gagatggcat ttgagacact tgcagtaatg
     3061 gcgggaacga cggatcttgc agcggttttg acgagcggcc agctctacat ctttgcgctg
     3121 gtatgcgctt tgtttattcc ctgcatttcc accatcgcag ttctcatgcg tgagaccggt
     3181 gtgcggtatg cagcgatgat taccgtcttt actttgtgtc tcggcattgg tctaggggcg
     3241 ctgacgcatc tgattttgct gtaatgagca agagctcatt acattccaaa ccctacagaa
     3301 tacatacatg ctgacattta tcggacttgg actctgcgat gaatatgatg tctccatacg
     3361 gggactggaa gcaatccgtc aggctgatgc ggtattcctt gaggtataca cctctgtgtt
     3421 aacagggacc accattccgc ggcttgagga gtattacgga aagaagatca ctccgctgta
     3481 ccgtgaggat gtggaactcc acccggagcc gattcttgat gcggcagcaa atggtgatgc
     3541 agtattttta accgctggag attcgatggt tgccacaacg catgctgata tccgtattcg
     3601 tgcggcagaa cggggtatcc ctacccgtat tattcatggt gcatccataa cgaccgcggt
     3661 atgcggtctg tctggtctgc aaaactaccg gtttggcaag tctgtttcgg ttccgtttcc
     3721 ttatggcaaa tggttcccga tgacaccgat tgaggtcatc tctgcaaatc tctcacaaaa
     3781 tctgcacacc ttggtgttcc ttgatattca gaaagacaaa gaacggtata tgagaattgc
     3841 agaagccgtt gatcttctgg aggagcaagc atcccgtgca ggaacatcta tccccttgta
     3901 tattggtatc gctcgtgccg gttccgaaaa tcctgttgtt cacgctggag atgcaaatga
     3961 tatgcgggag tttgagtttg gtgcgccgct tcacattatg gttgtccctg cggaactgca
     4021 tgatgttgaa cgcgaatatc tcgaacggtt tgccggatta tgctaatcga gtcttacgga
     4081 aaggagttta caactgactc ctctgctgca tgtcctgtag ctccagcatc caccccgctg
     4141 ggagcaactg ccgcagaaat tttggagatg gcctactgtt atgcttctga cgggatggta
     4201 ttttaccgga aaggtgatct tgttaatgcc gttgcatcct ttgcttatgg gtacgggtgg
     4261 ctggacgcag gtgtgtatct tgggtacgtg aatggtcggc ctgccacctc tcttccggtc
     4321 atttcggaac aaatccctga tacactattt gatcatctga gcgagaaaac aagccggtat
     4381 cagcgcatgc tttcttcagc acttgacggc gttgctgttc ttcctgatac tgagacacgg
     4441 gctttttctg ccgcgcagaa tattcggaca actgctgagg ttaactatgc tgagggaaca
     4501 acgctggttc ttgccaaaga tctgacgaat gcccttgtct ctttttctta cgggtacggg
     4561 tggctggatg ttggtgtccg tactggtctt tttggcatca ctggtgatcg acatctgttt
     4621 actatctgaa tcgttttttc aatacttttt taaaaatccc aagattcacg gggaaaaata
     4681 ttttgcaaag acagataagt atttattctc tgggcataat ctcttcatca tagagaaaga
     4741 ggtgatatca ccatgtcgga tatatacaag aaaatggttg aggaagcact ggccgcacag
     4801 tatgcggatg tgaacgtcct caaagagaag cggggtactc gtttctccct gtctgatgca
     4861 aaaccgtacg ttgacgcggt tgagaaaatg accgtcgcag acggacagag tgctgcagtt
     4921 atcaacctgc acaaagcttc tgttaagacc cattttgagg ttttatcagg actcacggga
     4981 tacgtccgcc ctgaggatga tccgttcgtg gagcactacc agactccggc aattcttgag
     5041 attctctgtg atgaagacaa aaagtttgcc aagagtcttg agacgtttgc ggaatcgatt
     5101 aaaaagaacg agaacattat tggtcgtgag gctgcccgct gctatgctgg gttctatggt
     5161 ccgtcctgtg tggtggactt tgccttaatg ccgggttcaa ccagcaatgt ggttaatcag
     5221 attcttcaga aaactgatat tccggtggat cacaagcagg caattcttgc cgcaaagtcg
     5281 tggggtatga acacgtccta cggtatcggt gatctgtttg caaaacggat tgaggctggt
     5341 gacacacttg ctgaggcttc cgacaaggag gtaaaacagc tccaggcact gtaccagcat
     5401 ccaattgatg cacaggttga gctgatgcaa aactccggca tgtcctcgtt tgacccgcga
     5461 aagtacatgg acaactaccg gaaaggcatg gagtctttcg tgaaggcagc aatcgatgac
     5521 ggagttcact acggtaacat cgcaacggtc ccagcatact gcgttggaga tatctcccac
     5581 cacatctccc agtccaccta caatatgtgc aaggatgatg tggtcatggc aacaatcgag
     5641 gcagtaactg gtgtcatcga caagacgtta catgcagcat tgccagaggc aaaaagcggt
     5701 tatcagctgc tggacgttgc caccggtgca tcagccgcag cggcagagta tctgcttgaa
     5761 ctggacggct ttaacgcacc gatgattgtg gatcttctga cgaaacgcta tcacaatc
//
LOCUS       DALUAJ010000043         6085 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_7896,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000043 DALUAJ010000000
VERSION     DALUAJ010000043.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7896"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="O0X37_00895"
     CDS             complement(<1..350)
                     /locus_tag="O0X37_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61518.1"
                     /translation="MKNIPLRIPAISLFMSVLVLVLTYVVVLSLWVNTLSADSVFIVF
                     CVLVLLGAFLYLALCPAMFVHGILNLMTLGQYPALTTKDYCNVLGRIFCITLVPIGIA
                     IVSTKMYNHPDMPY"
     gene            complement(350..835)
                     /locus_tag="O0X37_00900"
     CDS             complement(350..835)
                     /locus_tag="O0X37_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61519.1"
                     /translation="MITTTVWDIDGTLLVNPLPAPDWDNPQAVHPTTGENVCGDMPVY
                     FSHNQLNPQLRPKDLPEIDWVLTGRPRYRRLMTFDELGKYHINPLTLTLFPSEWQYTH
                     KKCMEWKADVLAVSFAADYYVDNDPQVAAEIIPLLKERKSHCQVITVSQWQQMFDDGV
                     I"
     gene            complement(835..1077)
                     /locus_tag="O0X37_00905"
     CDS             complement(835..1077)
                     /locus_tag="O0X37_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61520.1"
                     /translation="MGRRKYILSYRSPPECVWDNVIMVGSAGCCRGIYRYYQQYGRPD
                     SPLVSKIKVLSGMATRIIPLPSCLRWGRPDNMWRCT"
     gene            complement(1080..1898)
                     /locus_tag="O0X37_00910"
     CDS             complement(1080..1898)
                     /locus_tag="O0X37_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HJK61521.1"
                     /translation="MTIQTRNRKKIETNILYEGDCLEVMKSLPDKSIDCILTSPPYWN
                     TFWEKYSTDPAMVSIEPDITSYLSHLVSICKECYRVLRDTGVFWVLIGEPNLRNGKRR
                     SNLSGIPWKLAFSLQDIGWILRSDTIAKLHFDPAKYDLEKAKFDLDDGRPWCTHHYVF
                     MFTKKLNHYWDGNQYLHSGDVWEISPVNSVDGFTFETSAPDLVKTCICCGCHPEGIVL
                     DPFCGSGTSCIVANSEQRKFIGVELVHETAVKAFKRISSYAENARFYVFDYGGV"
     gene            complement(1895..2371)
                     /locus_tag="O0X37_00915"
     CDS             complement(1895..2371)
                     /locus_tag="O0X37_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61522.1"
                     /translation="MTIINLHFLPHLAAANIAGTKSRTTRTKPHGKPGDIFFCKGMAF
                     VITKIEPERLIDAAYHFKEEGYLKKIDFLAELKNIYPELYYETLTMMMSDSTHLWQKY
                     CMGQLTDEQQKRYEERIRKRYKTILYAHHYQKYGHVDITAIRGIPPVYWHQEVASL"
     gene            complement(2368..2916)
                     /locus_tag="O0X37_00920"
     CDS             complement(2368..2916)
                     /locus_tag="O0X37_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61523.1"
                     /translation="MSVQVRRVNNPEECPFRSEGYRNIFDITDEGYRCSISTKCCKEQ
                     LREGNCPLKCTDILVKGEHSGSRTPQLHGNLSRAQKILDNIEHLTELTLFLVIEYDSS
                     DLRNGLPGYRACLDIGRLVEPSHDEVELCGYGYTPSDAVINYVQNLLGKQILYLTEDG
                     DTIGQFYLDTDGIVSKWEEVQI"
     gene            complement(2913..3506)
                     /locus_tag="O0X37_00925"
     CDS             complement(2913..3506)
                     /locus_tag="O0X37_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61524.1"
                     /translation="MTKNAEITRSIILKMYTSGKQHSRDDILGFTRDICPDPTPQQIY
                     NIDCIIKALVDTGIFYQTHSSDYRNIKYSLNLELFKMIGMPVYRRNPVLEYQSFVPEA
                     CALMDLLTLNNGGPVTLIAAEELCSRIPNMTGDPLSLCDVIKRYPDIITEYAEVDTDG
                     DEYAFIEFTCPFPPDDTGEALYRLMAAALKTKGVDEK"
     gene            complement(3503..3676)
                     /locus_tag="O0X37_00930"
     CDS             complement(3503..3676)
                     /locus_tag="O0X37_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61525.1"
                     /translation="MTDTLPLTPDQLKLLVAIGAGFALGMLSGVALMWTSLPQVFTLL
                     GINDLVVLGCTLP"
     gene            complement(3673..3987)
                     /locus_tag="O0X37_00935"
     CDS             complement(3673..3987)
                     /locus_tag="O0X37_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61526.1"
                     /translation="MNLNYEIAFTLAKNGNQPQTPAQIADEILKTIDQTIDLAPEAEA
                     VKGVIETTLDTLCLFGMYTTPAPGTYALTDIWYASLTPCEGCRIREEHYAILRHLRGG
                     RA"
     gene            complement(3997..4185)
                     /locus_tag="O0X37_00940"
     CDS             complement(3997..4185)
                     /locus_tag="O0X37_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61527.1"
                     /translation="MNKSRSISPNQPTKDDNQTDIYTSAPNHKKLSTIIDADARQARK
                     LLHLRIRAEPALRDIIIA"
     gene            complement(4182..4397)
                     /locus_tag="O0X37_00945"
     CDS             complement(4182..4397)
                     /locus_tag="O0X37_00945"
                     /inference="COORDINATES: protein motif:HMM:NF025712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HJK61528.1"
                     /translation="MTSSTPAKTLSPCPFCGSSILHYSYPGYGGRVQIICDICGARGG
                     IAKTEPEARRAWNTRTIEKPKKQEKTA"
     gene            complement(4400..4585)
                     /locus_tag="O0X37_00950"
     CDS             complement(4400..4585)
                     /locus_tag="O0X37_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61529.1"
                     /translation="MTTDPTISAILTASPDNPVEVKMSVYLKIKQNHPGTAYKTLRKM
                     LAEHSAFLIPDLPTTKA"
     gene            complement(5120..5752)
                     /locus_tag="O0X37_00955"
     CDS             complement(5120..5752)
                     /locus_tag="O0X37_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61530.1"
                     /translation="MLQFWRNHKFREVEINPEHHAFAQARWDAWIHSPEYEKIFRQYF
                     REYDGQQLAGDHDFDDWNYQQFVRSAAYNQALAGWVWCRKYDEEKERQKEHEAYIEAA
                     LKNEYMIWLNSVLLKTSLVNYWLLEVDTKWWGDEKNSIIYNVLLRRHDLTPYVSDERL
                     LNPFIKELSDLTGWEWEKKEAVDALYQWYDWAWIWYQHKYDITLTPIEHD"
     gene            complement(5810..6040)
                     /locus_tag="O0X37_00960"
     CDS             complement(5810..6040)
                     /locus_tag="O0X37_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61531.1"
                     /translation="MTSKRDTQRAVCRAEHDQHNAAADARPGCGDSGATPARRTDNGT
                     PSDELCEQIRSLFREILKEPEFSGRFRRKKEM"
BASE COUNT         1457 a         1376 c         1565 g         1687 t
ORIGIN      
        1 atgtaaggca tatccgggtg attgtacatc ttggttgata cgatcgcaat cccgatcggg
       61 accaaagtga tacagaagat tctgcccagt acattgcagt agtcttttgt tgtgagtgcc
      121 ggatactgcc caagagtcat gagattgagg atcccgtgaa cgaacattgc ggggcagagt
      181 gcaagataca gaaatgctcc gagcagcacc aatacgcaga acacgatgaa tacgctatca
      241 gccgagagtg tgtttaccca tagagagagt acaacaacat acgtcagcac cagcaccagc
      301 acagacataa acagagatat agccgggatt ctgagaggta tgttcttcat cagatcaccc
      361 cgtcgtcaaa catctgctgc cactgcgata ctgttatcac ctgacagtga gatttccgtt
      421 ctttgagcag cgggatgatt tcggcggcta cctgcggatc attgtcaaca tagtaatccg
      481 cggcgaagct gacggcgaga acgtccgctt tccattccat acattttttg tgagtgtact
      541 gccattcgga cgggaagagt gtgagagtga gcgggtttat gtgatatttt cccagctcgt
      601 cgaaggtcat gagccgtcga taacgcgggc gcccggtaag tacccagtcg atctccggca
      661 gatcttttgg tctaagctgc gggttgagct ggttgtgaga gaagtacacc ggcatgtcgc
      721 cgcagacgtt ttccccggtt gtgggatgga ctgcctgagg gttgtcccag tccggagcgg
      781 gcagcggatt taccaggaga gttccgtcga tgtcccagac ggttgtagta atcattacgt
      841 acacctccac atattatcag gccgccccca cctgagacag cttggcagcg gtattatacg
      901 tgtcgccatt cctgagagga ctttgatctt agagacaagc ggcgaatccg ggcgaccata
      961 ctgctgataa tagcggtaaa tgccgcgaca acaccctgcg gatccaacca taatgacgtt
     1021 gtcccacaca cattcggggg gggaacgata cgataagatg tattttcgac gacccatgtt
     1081 tagacccccc cataatcaaa aacatagaac cttgcatttt ccgcataact tgagatccgt
     1141 ttgaatgctt tgacggcggt ttcgtgaact aattccacgc caataaattt tctctgttca
     1201 gaattggcaa caatacaaga agttccgctg ccgcagaatg gatccagtac gataccttcg
     1261 ggatgacacc cacaacaaat acatgttttg accaaatcag gtgccgaggt ttcaaaggta
     1321 aaaccatcaa cagagttgac aggcgaaatc tcccaaacgt ctcccgaatg aagatactga
     1381 ttaccatccc agtaatgatt cagttttttt gtaaacatga atacatagtg atgcgtacac
     1441 cagggacgac catcgtctaa gtcaaatttt gccttttcaa gatcgtattt tgcgggatca
     1501 aaatgcagtt tcgcgatcgt atctgatcgg agtatccatc cgatatcctg gagggaaaag
     1561 gcaagtttcc acggaatgcc ggataggttt gatctacgtt taccatttcg caggtttggc
     1621 tctccaataa gaacccagaa aacacccgta tcccgaagaa cccggtagca ttctttacaa
     1681 atactgacga gatgtgaaag atacgaggtg atatcaggtt ctatagacac catagcggga
     1741 tcggtagaat acttttccca aaaggtgttc caataaggcg gactggttag gatacagtcg
     1801 atagatttgt ctggaagaga tttcatgact tccaggcaat caccttcata aaggatgttt
     1861 gtctctattt ttttccggtt gcgggtttgg atagtcataa tgatgccacc tcctgatgcc
     1921 agtataccgg cgggattccg cgaattgctg tgatgtccac atgaccatat ttctggtaat
     1981 ggtgagcata caggatggtt ttgtaccgtt tgcggatcct ctcttcgtag cgtttctgct
     2041 gttcgtcggt taattgtccc atgcagtatt tttgccataa atgggttgaa tcactcatca
     2101 tcatcgtgag agtttcgtag tagagctcgg gatagatgtt cttgagttct gcgagaaagt
     2161 cgattttttt cagataccct tcttctttga agtggtatgc agcgtcgatg agccgttctg
     2221 gttcgatctt tgtgatgacg aaggccattc ccttgcagaa gaatatgtct cccggttttc
     2281 cgtgcggttt tgtgcgggtg gtgcgggatt tggttccggc aatgtttgcg gctgcgagat
     2341 gcggcaggaa gtgaagattg atgatggtca tatctgcacc tcttcccatt tcgacacgat
     2401 cccatccgtg tcaagataga actgacctat ggtatcgcca tcttcagtga gatacagaat
     2461 ctgttttccg agaaggttct gtacgtaatt gatgactgcg tccgagggag tgtatccgta
     2521 tccgcagagt tctacttcat cgtgggatgg ttcaaccagc cgaccaatat cgaggcaagc
     2581 cctgtagccg ggaagaccat ttctgagatc tgaggagtcg tattcgatga cgaggaacag
     2641 tgtcagctcc gtcaagtgct cgatattgtc gaggattttt tgtgcccgtg ataaatttcc
     2701 gtggagctgc ggagtgcggg agccggagtg ttcgcctttg accaggatgt cggtgcattt
     2761 tagcggacag tttccttcac gcagttgttc tttacaacat tttgttgaga tgctgcagcg
     2821 gtatccttcg tcggttatgt cgaatatgtt cctgtaccct tcagatctga acggacattc
     2881 ttccgggttg tttactctcc gtacctggac gctcattttt catccactcc ttttgttttc
     2941 agtgcggcgg ccatgaggcg gtagagagct tctccggtgt cgtccggagg gaaggggcag
     3001 gtaaattcga tgaaggcata ctcatccccg tccgtgtcaa cttctgcgta ttcggtgatg
     3061 atgtcagggt agcgttttat cacatcgcag agggagagcg gatctcctgt catattcggg
     3121 attctggaac acagttcttc ggctgcgatg agtgttaccg gccctccgtt gttgagggtg
     3181 agcaggtcca tgagggcgca ggcttccggt acaaaagatt ggtattcgag taccgggttt
     3241 cttctgtaca ccggcatgcc aatcattttg aaaagttcca ggttcaggga gtatttgatg
     3301 ttccgatagt ctgagctgtg agtctggtag aagatgcctg tgtccaccaa tgctttgatg
     3361 atgcagtcga tgttgtagat ttgctgcggt gttggatccg ggcagatgtc ccgggtgaat
     3421 ccgaggatgt cgtcccggga gtgctgtttg ccggaggtgt acattttcag gatgatggat
     3481 ctggtgattt ctgcattttt tgtcatggta gtgtgcaccc caatactacc aggtcattaa
     3541 ttcccagaag agtgaaaacc tgcggcagag atgtccacat cagagctacg ccagagagca
     3601 tccccagcgc aaaacctgcg ccaatggcta cgaggagttt cagctgatcg ggtgtcagcg
     3661 ggagtgtgtc agtcatgctc ttcccccccg caggtgccgc agtatggcat agtgttcttc
     3721 tctgattctg catccttcgc atggtgtgag ggatgcatac cagatgtcgg tgagggcata
     3781 ggtgccgggt gcgggggttg tgtacatgcc gaagagacac agggtgtcaa gagttgtttc
     3841 gattacgcct tttactgcct cggcctcagg ggcgaggtct atggtttggt cgatggtttt
     3901 gaggatttca tcggcgatct gagcgggtgt ttgcggctgg tttccgtttt tggcaagcgt
     3961 gaatgcgatt tcgtaattga gattcatgat gttcacctat gcgatgatga tgtcacgcag
     4021 agccggttct gcacggatgc ggaggtggag gagtttgcgt gcctggcgtg cgtccgcgtc
     4081 gatgatggtg gaaagttttt tgtggttcgg tgcggatgtg tatatgtcag tctgattatc
     4141 atctttggtc ggttgattag gactgatact tctgcttttg ttcatgcggt tttctcctgt
     4201 tttttcggtt tttctatggt tcgtgtgttc catgccctgc gggcttcggg ttctgttttg
     4261 gctatgccgc cgcgtgctcc gcagatgtcg cagatgatct ggacgcggcc gccgtatccc
     4321 gggtatgagt agtggaggat gctcgatccg cagaagggac acggtgatag ggtttttgct
     4381 ggtgtgctgc tggtcatgat tacgccttgg ttgtcgggag gtcggggatc aggaatgcag
     4441 agtgttctgc aagcattttc cgcagggttt tgtatgcggt tcccgggtgg ttttgtttga
     4501 ttttcaggta cacggacatt ttgacctcta ccggattgtc cggagatgcg gtgaggatgg
     4561 ctgagattgt tggatcggtt gtcatggttc tatctccgaa tatttgccag ctcttgatcc
     4621 acacacttcc ctacggcata tcgaatataa tcaggtcgtg acagtctcag tggtgcccta
     4681 cacagatcga ttttttcgat gtctgcttta gataagcgga ctgacatagg agtggtctta
     4741 tactctgtag tcataagtag tcatttgcac tacatgcaga taaatgtatt cataagtagt
     4801 cataagtagt cataacaaat gtagcccatc gcgaccacat atacaacaaa tggataaagg
     4861 aaagaataca gatcagctca caatacgcat atcaacccgt ttaagagaag attcagaaag
     4921 atgcgccata aatgcaggta tatcgcatgc agaatggata cggcgcgcac ttcaagcaca
     4981 agttgagaga gataccaaca acaaccaacg ctccgaaaca atagatcaag tacgtgaaat
     5041 aatccacgaa gaactcagaa attacgagag agtggatgaa gagagatgag ccgtacaaaa
     5101 taaagaaatg attatgatat taatcatgtt caatcggagt taacgttata tcatatttat
     5161 gctgatacca aatccacgcc cagtcatacc actgatagag tgcatcaacc gcctctttct
     5221 tttcccattc ccaacccgtt agatcagata attcctttat aaacggattt aggagtctct
     5281 catccgaaac atacggagtt agatcgtgac gacgcaataa aacgttatag ataatggagt
     5341 ttttctcatc accccaccac ttagtatcta cttcaagaag ccaatagtta actagtgatg
     5401 ttttcaataa cacactattt aaccatatca tgtattcgtt tttcaaagct gcttcaatat
     5461 aagcttcatg ctctttttgc ctctctttct cctcatcgta cttcctgcac cacacccatc
     5521 ctgcaagtgc ctgattatag gcagccgacc gcacaaactg ttgataattc cagtcgtcaa
     5581 agtcatggtc gcctgcaagc tgctgaccat catactcacg gaaatactga cggaagattt
     5641 tctcatattc cggagagtgg atccacgcat cccaccgagc ctgtgcaaat gcatgatgct
     5701 ccgggttgat ctcaacctcc cggaacttgt gattcctcca gaactgtaac atgaaaaaga
     5761 ggttgcacac agaatacaat aaaaggtgcg gtagggataa aaaagagatt tacatctctt
     5821 tttttcgtct gaatctgccg gagaactccg gttccttcag aatctcccga aacagggatc
     5881 ggatctgttc gcataactca tcggatggcg tgccattgtc tgtcctgcgt gccggggtag
     5941 ctccagaatc tccgcatccc ggccgggcat ctgctgctgc gttgtgttgg tcgtgctccg
     6001 cacggcagac ggctcgctgt gtatctcgct ttgaggtcac aacgcttacc tctacccaat
     6061 catcgataac ggatcagata aagac
//
LOCUS       DALUAJ010000044         1885 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_8000,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000044 DALUAJ010000000
VERSION     DALUAJ010000044.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8000"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="O0X37_00965"
     CDS             complement(<1..1152)
                     /locus_tag="O0X37_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HJK61532.1"
                     /translation="MKACIMCGGEGTRLRPLTFERPKPCIPIVNRPSIAHLVTHLANL
                     GFTDIVITIGYLGDEIQKALGDGSLYGATITYVPEKIKLGTAGSVKNAEEYLNDAPFL
                     VVGGDHMTDLNVLEFYREHMNSSAITSIGLISIEDPREFGIAEIDASLRIRRFREKPS
                     PGEIFSNLASTGIYVCDPKIFEFIPKDTKFDFAKDLFPLLMEKGYQLNGWLARGNWTD
                     VGNPAMLRAAEKWKLQENATTSIAGTLNVKDAHITGPVRFGDGITLGAGSRIVGPVFI
                     GSGVTIGENVIIGPYTSIGENVLIKNNAKIFSSSIYNGVVIGANTTISGSIVDIDTII
                     GDNCSIEHNTVIGPRSVLRNNVTIHSGTRLWPEVIVPEKSIVKEHMLNEK"
     gene            1434..>1885
                     /locus_tag="O0X37_00970"
     CDS             1434..>1885
                     /locus_tag="O0X37_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HJK61533.1"
                     /translation="MDLDTGATAWVLISAALVLLMVPAVGLFYGGMVRKKNVIATMML
                     SLVALALGVIQWIIIGYSLAFGSDVGGFIGNLEYFGLNGITLDGVSGGIPDMLFVCFQ
                     MMFACLTLAILTSGVVGRIKMSAFLIFGAIWLTIVYAPLAHWAWGGGWA"
BASE COUNT          434 a          407 c          479 g          565 t
ORIGIN      
        1 cttttcgttg agcatgtgtt ctttaacaat cgatttttcc ggtacgatga cctccggcca
       61 gagcctggta cctgagtgga tagttacgtt gttgcgcaga actgatctcg gaccaatcac
      121 agtgttgtgt tcgatggaac agttgtcgcc gatgatggtg tcaatgtcaa cgatgcttcc
      181 cgagatggtt gtgtttgcac cgataacaac accgttgtaa atagatgagg agaatatctt
      241 cgcattgttt ttgatcagaa cattttctcc gatgctggtg taaggaccga tgataacatt
      301 ctctccgatg gttacaccac tgccgatgaa gactggacct acaatacgtg aacctgctcc
      361 aagcgtgatg ccgtctccga accgcaccgg gccggtgata tgtgcatctt tgacgttgag
      421 cgttcctgca atgctggttg ttgcattctc ctggagtttc catttttctg ccgcgcggag
      481 cattgcagga ttgccgacat ctgtccagtt gccgcgtgcg agccaaccat tgagctgata
      541 ccccttttcc attaaaagcg ggaagaggtc tttggcaaag tcgaacttgg tgtctttggg
      601 gatgaactca aagatctttg gatcacagac ataaattccc gtacttgcaa ggtttgagaa
      661 tatctctcct ggagagggtt tttcacggaa tctgcggata cgaagggatg cgtctatctc
      721 ggcaatacca aattcgcggg ggtcttcaat gctgataaga ccgatcgagg tgatcgcaga
      781 ggagttcata tgttcacgat agaactcgag aacgttgaga tcggtcatgt gatctccgcc
      841 gaccacaagg aacggagcat cgttgagata ctcttcagca tttttgacag accccgctgt
      901 tccgagtttg atcttttcgg gaacataggt gatagttgct ccgtaaagtg atccgtcacc
      961 aagtgctttc tgaatctcat caccgagata tccaatggtg atgacaatat cggtaaaacc
     1021 aagattggca aggtgtgtaa ccagatgggc aatagacgga cggtttacta ttggaataca
     1081 cggctttggc cgttcaaatg tcagagggcg aagacgcgtt ccctcgccgc cgcacataat
     1141 gcaggctttc atattactct ataatatgcg ctggtatcta ttggttgtat ctttccttct
     1201 gcgcgtaggt attggaaatc tctaaaatac acactgtgtc atgtcacacg ttagcatgag
     1261 acatgttatt ttatatatct aaaccatcaa cattggtact agcacaagac accacaaaag
     1321 gtttagagta gaatgggatt ttcctctgcc tgtggtgttt aaaccatggg attttgggga
     1381 ttcttttctg ctttgggctt ttgtatctgt gcagtgctaa ttggtgaatt actatggatc
     1441 ttgatactgg tgcgacagcc tgggtactta tttcagccgc attagtgttg ctgatggttc
     1501 ccgcagtagg tttgttctac ggtggaatgg taagaaaaaa gaacgttatc gcaactatga
     1561 tgctatccct tgtggctctt gcccttggcg tgatccagtg gattatcatc ggctattcac
     1621 ttgcctttgg cagtgatgtt ggcggattca ttggaaacct ggagtacttt ggtttaaacg
     1681 gtatcacact tgatggtgtg tcaggaggta ttcccgacat gttgttcgtc tgtttccaga
     1741 tgatgtttgc atgtctgaca ctcgccatcc tgacctctgg tgttgttggc agaattaaga
     1801 tgtctgcgtt cctcatcttt ggtgccattt ggttgaccat cgtttacgcc ccgcttgcac
     1861 actgggcatg gggaggagga tgggc
//
LOCUS       DALUAJ010000045         5764 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_8259,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000045 DALUAJ010000000
VERSION     DALUAJ010000045.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5764)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5764)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5764
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8259"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="O0X37_00975"
     CDS             complement(<1..430)
                     /locus_tag="O0X37_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJK61534.1"
                     /translation="MRITISGSPGSGTTSLGKALAEKYNLRYLSAGEVFRGLAKEHGM
                     DLAAFGKLAEENPEIDLKIDARQKEIGEASDDIILEGRLAGWMVENADLKILLYASPD
                     CRAERIAERENLTPEEACSATIEREACEAARYMEYYEIDIA"
     gene            complement(434..1063)
                     /locus_tag="O0X37_00980"
     CDS             complement(434..1063)
                     /locus_tag="O0X37_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EMC3/TMCO1 family protein"
                     /protein_id="HJK61535.1"
                     /translation="MEFGKKYGMYIALAVVFGMMLLYGWPEARTFISGIVDIVIGPFA
                     ALGLPFFALVLILATLTGLYSSLIQKYTIDYEKMRENQEKFKEFNVLFREAQRSGDEK
                     AIKKMQARQQAMMAEQMEMSQQQFKPMAYILVLTVPIFFWLIEHIPQNVDLTMAHADL
                     SNAIILPFAGLSSYFDIYLGFFPLWILWYMLCSITVSQVIRKALNIGGM"
     gene            complement(1070..1642)
                     /locus_tag="O0X37_00985"
     CDS             complement(1070..1642)
                     /locus_tag="O0X37_00985"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HJK61536.1"
                     /translation="MAGKKVIITGVPGVGKTTVINTAMEKIAAEGTPYQNINFGSFMF
                     EVAAAEGLVQDRDQMRKLDRTVQKRLQKLAAEKIAAIDGNVIIDTHASVKTPNGYLAG
                     LPEWVITAIMPDVLVLVETDNDQILVRRLSDETRVRDMEGAKSIAEHQEMNRAFAAAY
                     AMLTGCTVKIIVNADFLLDKAVEELTATLR"
     gene            complement(1848..3290)
                     /gene="secY"
                     /locus_tag="O0X37_00990"
     CDS             complement(1848..3290)
                     /gene="secY"
                     /locus_tag="O0X37_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HJK61537.1"
                     /translation="MGELLDRMEPLLAKMPAVRPPEGHVHFKNKLMWTAAVLLLYFIL
                     TNIPVFGLSETSLDVFQYYRALLAGASGTILHLGIGPIVTASIVLQLLRGADLIKINT
                     SDERGQVIYMGLQKLLIFVMIILEALPNVLGGWMSPDMTVAAMFGGNTAAVMFLIFLQ
                     ICLGGLLVVFMDEVVSKWGIGSGVGLFIVAGVAQGLVNGFFNWEATTDQFAVGFFPRL
                     FEVISSGANFVEYFGLELLALFTTVGLFFLIVYVEATRVEIPLAHANVRGARARFPVK
                     LVYASVLPMILVRVLQANVQMIGMFLSSVGINIFGEFNGSTPINGLMWYLAPINQPQD
                     WMWWHPQFTGVGHAAWEVAIRVGIDAVVMIVGGALFAIFWVKTAGLDSKHVARQIQNS
                     GMQIPGYRRSPAVLERYLDRYIPRVTVIGGVFIGVLSILANMLGIIGFVGGTGLLLTV
                     SIVYRLYEQIANEQIMEMYPFMRGFFGGKE"
     gene            complement(3315..3737)
                     /locus_tag="O0X37_00995"
     CDS             complement(3315..3737)
                     /locus_tag="O0X37_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HJK61538.1"
                     /translation="MPVNKRSKFRGTRTCGGGTHKNRRGAGNRGGRGQAGWRDHNFTR
                     FYLLGKTEGKHGFVCKTSVTYEVLDIGDIDQMVPDLLARGIATQNGDVIILDAAQIGV
                     EKVLGGGRVTHKLEITAESFSERAIAKVQEQGGQALTP"
     gene            complement(3743..4204)
                     /locus_tag="O0X37_01000"
     CDS             complement(3743..4204)
                     /locus_tag="O0X37_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HJK61539.1"
                     /translation="MYAVVQVRGVVNTRRDIKETLKMLRLHHINHCVLVPETPEYLGM
                     IRKAKDYIAFGEVDAATLETILTTRGRLTGNKPLTDEYVKEATSFGSIAELAAALAAG
                     QIRMKDVPELKPVLRMHPPRKGYKTTKRTFNQGGALGYYGKEINDLLIKMR"
     gene            complement(4204..4821)
                     /locus_tag="O0X37_01005"
     CDS             complement(4204..4821)
                     /locus_tag="O0X37_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HJK61540.1"
                     /translation="MAYEQEEWVPITGLGKKVMAGDFASFDEILASGYPIKEAGIVDA
                     MLPDLVDEVLCIDMMQRMTDSGRRIKFRAVVVVGNKDGYIGFGQGRDVQVGTAIKKAI
                     VNAKLNIVKVRRGCGSWECGCGQKHSVPMEVTGKAGSVSVTLKPAPKGIGLVTGDVGK
                     KVLALAGIRDVWVNTSGNTRTTLNFAKATYNALRETNLIRIGGRK"
     gene            complement(4823..5347)
                     /locus_tag="O0X37_01010"
     CDS             complement(4823..5347)
                     /locus_tag="O0X37_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HJK61541.1"
                     /translation="MATNGRYFVQFRRRREGKTDYYQRQRLIVSGRNRMVIRKTNRHI
                     IIQLVAAQMDGDYTLVHVNSKELVNYGYKGYLGNTPAAYLTGMLFAVRAQKAGYEGAI
                     ADIGLQVASTGARVFAAVKGAVDAGFDVPVGESILPDDDRCNGAHIAEYDERYADLVS
                     QVEAAKDAIMKELE"
     gene            complement(5351..>5764)
                     /locus_tag="O0X37_01015"
     CDS             complement(5351..>5764)
                     /locus_tag="O0X37_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19e"
                     /protein_id="HJK61542.1"
                     /translation="VLGCGVNRVWINPEKLSEVQSAMSREDIRNLIEEGAISSHQKKG
                     ISRGRARARIAKRAYGHCKGPGRRSGAKGARTPSKTQWIKKIRAQRKELRAQRDAGSI
                     TRSEYRRLYRRAAGGQFRNVAHLKAQIEIVTSRRD"
BASE COUNT         1458 a         1575 c         1375 g         1356 t
ORIGIN      
        1 cagcgatgtc aatctcgtag tactccatgt accgtgctgc ttcacaggct tcacgctcaa
       61 tggttgcgga acatgcctcc tcaggagtca gattttcacg ctcggcgatc cgctcggcac
      121 ggcagtccgg cgatgcatac agcaggattt tcagatcagc attttccacc atccatccgg
      181 cgagacgccc ttccaggatg atatcgtcac tggcttcccc aatttctttc tgccgggcat
      241 caatcttgag atcaatctca gggttttctt cagcaagctt tccaaatgct gcaagatcca
      301 tcccgtgttc tttagcgaga ccacggaaga cctcaccggc ggagagatag cggagattgt
      361 atttctcggc aagggctttc ccaagagagg tagtccctga gcccggggat ccgctgatcg
      421 taatccgcat cgattacatg cctccaatat tgagggcttt tctgatgacc tggctgaccg
      481 taatggaaca gagcatatac cagagaatcc aaagcgggaa gaatccaagg tagatatcaa
      541 agtaactgga aagtcctgca aacggaagga taatggcgtt actcagatct gcgtgtgcca
      601 tggtcagatc aacattctgc gggatgtgtt cgatcagcca gaagaagatc ggcaccgtca
      661 gaacaaggat gtaagccatg ggtttgaact gctgctggct catctccatc tgttcagcca
      721 tcattgcctg ctggcgcgcc tgcatcttct tgatggcttt ctcatcgccg gaacgctgtg
      781 cttcacggaa cagaacgttg aactccttga atttttcctg gttctcacgc atcttttcat
      841 agtcaatggt gtacttctga atcagcgagg agtacaaacc tgtaagtgtt gcaaggatca
      901 ggacaagtgc gaagaacgga agaccaagag cggcaaaggg accaattaca atatcaacaa
      961 tgccggagat gaaagtacgt gcttccggcc agccgtagag aagcatcatg ccgaacacta
     1021 cggcgagggc gatgtacatt ccgtactttt tgccaaattc catgaaaggt tacctcaggg
     1081 ttgcagtaag ttcctcaaca gctttgtcaa ggaggaagtc agcattaacg atgattttca
     1141 ccgtacagcc ggtcagcatc gcataggctg cagcaaatgc acgattcatt tcctggtgtt
     1201 ctgcaatgga ctttgcacct tccatgtcac gtacacgagt ctcgtcggac agtctgcgga
     1261 cgaggatctg atcgttgtcc gtctcaacaa gaacgagaac atccggcatg attgcagtga
     1321 tgacccactc aggaagaccg gcaagatatc cgttcggcgt cttaacagat gcatgagtgt
     1381 cgatgatgac gttcccgtca attgcagcga tcttttctgc agcgagcttt tgcaggcgct
     1441 tctgaaccgt acggtcaagt tttctcatct ggtcacggtc ctggacaagg ccttccgcag
     1501 cagcgacttc aaacatgaaa ctgccgaagt tgatattctg gtagggagta ccttctgcgg
     1561 cgatcttttc cattgcggtg ttaattacag ttgttttgcc gacaccgggc acaccggtga
     1621 taatgacctt ttttcctgcc atattggatc acttttccta tgtgagaatc gtttaaacgt
     1681 tggtgggaca ggataataaa tctatttttt ggaaggagag gatgtgtatt ttccagaaca
     1741 agaaaggaaa agaaatactc acacacatct gcaattttgc agatttgcat gcatataaaa
     1801 gaaaaatcca aacctaagac agagtaaaag attcagaaaa aatggggtta ttctttgccg
     1861 ccgaagaagc cccgcataaa cggatacatc tccatgatct gctcgtttgc aatctgttca
     1921 tacagacggt aaacgattga gaccgtaagc agcaaaccag tacctcccac aaagccaatg
     1981 atacccagca tgtttgcaag aatacttaag actccaataa acacaccgcc aatcactgtg
     2041 acacgcggga tgtatctgtc cagataccgc tccagcacag cggggctgcg gcggtaaccg
     2101 ggaatctgca taccagagtt ctgaatctgg cgggcaacat gctttgaatc aagacctgct
     2161 gtcttaaccc agaagattgc gaacagagca cctccgacaa tcatgacgac tgcatcaata
     2221 cccacacgga tagcaacctc ccatgctgca tggccgacac ccgtaaactg cggatgccac
     2281 cacatccagt cctgcggctg gttaatcggt gcaagatacc acatcagacc attgatcggc
     2341 gtggaaccgt tgaactctcc aaagatgttg ataccaacac tggaaaggaa cataccaatc
     2401 atctgaacgt ttgcctgcag aacacgcacc aagatcatcg gcagaacact tgcatacaca
     2461 agttttaccg ggaaccgggc acgggcacca cgcacatttg cgtgagcaag cggaatctcg
     2521 acacgggtcg cctcaacata gacgataagg aagaacaatc ctactgtcgt gaacagtgcg
     2581 agcagctcaa gaccaaagta ctcaacgaag ttcgcaccgg agcttatcac ttcaaacaga
     2641 cgggggaaga aacccaccgc aaactgatcc gtcgtagcct cccagttgaa gaaaccgttc
     2701 acgagaccct gggcaactcc tgcaacgatg aagagaccga caccagaacc aataccccac
     2761 ttcgacacaa cttcgtccat gaacacaacc agcagaccgc ctaaacagat ctgcaggaag
     2821 atcaggaaca tcacagctgc ggtattgccg ccaaacattg cagcaaccgt catgtcaggg
     2881 ctcatccatc cgcccagaac gttcggcagg gcttccagga taatcattac aaagatcagc
     2941 agcttctgca gacccatgta gataacctga ccgcgctcat cggaagtatt gatcttgatc
     3001 aggtcagcac cacgaagcaa ctgcagtacg atagatgcgg tcacaatcgg tccgatacca
     3061 aggtgaagaa tcgttccgga tgcaccagcg agtaacgcac ggtaatattg gaaaacatcc
     3121 agagaagttt cactcagacc aaaaaccgga atgttcgtca gtataaaata caacagcagc
     3181 accgcggcgg tccacatcag cttgtttttg aagtgaacat gaccctccgg tggccgaaca
     3241 gctggcatct ttgccagcag tggttccatt cgatccagca gttctcccat gagtgataca
     3301 ccaaaaattt aaatttaggg ggtcagtgcc tgaccgccct gttcctgaac ttttgcgata
     3361 gcacgctcgg agaagctctc tgcagtgatc tcaagcttgt gggttacacg accaccgccg
     3421 agaaccttct caacaccaat ctgtgcggcg tcaagaataa taacatctcc gttttgtgta
     3481 gcaataccgc gggcaagaag atccggaacc atctggtcga tatcaccgat atcgagaact
     3541 tcgtaggtaa cagaggtctt acagacaaat ccgtgtttac cctccgtctt tccgagcaga
     3601 tagaaccggg tgaaattgtg gtcacgccat cctgcctgtc cacgacctcc gcggttacct
     3661 gcaccacggc ggttcttgtg ggttccccca ccacaggtgc gggttccccg gaactttgaa
     3721 cgcttgttga ctggcatact ttttacctca tcttgatcag aaggtcgtta atctccttcc
     3781 cgtagtatcc cagggcaccg ccctgattga acgtacgctt tgtggtcttg tatccctttc
     3841 ttggcgggtg catacgaagc accggcttga gttcaggaac atccttcatc cggatctgac
     3901 ctgctgcaag agcagcggcg agctcggcaa tgcttccaaa ggaagttgct tccttgacgt
     3961 actcatcagt caggggcttg tttccggtaa gtcttccacg ggtagtaaga atcgtctcaa
     4021 gcgttgctgc atcaacttca ccgaatgcga tgtagtcctt tgccttccgg atcataccaa
     4081 ggtactccgg agtctccggc acaaggacac agtggttgat gtggtggagg cgcagcatct
     4141 ttaaggtctc tttaatgtca cggcgggtgt tcaccactcc gcgaacctgc actacagcgt
     4201 acattacttc cgacctccga tacgaatcag attcgtttca cgaagtgcat tgtaggttgc
     4261 ctttgcgaag ttcagtgtcg ttctggtgtt accgctcgtg tttacccaca catcacggat
     4321 acctgcgagt gctaatacct tcttaccgac atcaccagtc acaagaccga ttcccttcgg
     4381 ggcgggcttg agggtcacgc tgacggaacc tgccttacca gtaacttcca tcgggacaga
     4441 atgtttctga ccgcagccgc attcccaaga accgcatcca cggcgaacct tcacaatgtt
     4501 cagcttcgcg tttacgattg ccttcttgat cgcagttccc acctggacat ctctgccctg
     4561 gccgaaaccg atgtaaccat ccttgttgcc gacaacaacg accgctctga acttaatacg
     4621 gcgaccggag tcagtcatac gctgcatcat gtcaatgcag aggacttcgt caacaaggtc
     4681 aggaagcata gcatcgacaa tacctgcttc cttgatgggg taaccacttg caagaatctc
     4741 atcgaagctt gcaaagtccc cggccatgac tttcttgcca agaccggtga tggggaccca
     4801 ctcttcctgt tcgtacgcca tattactcca gctccttcat aattgcgtcc tttgcagcct
     4861 cgacctgaga gacaagatct gcataacgct catcatattc tgcaatgtgg gcaccgttgc
     4921 agcggtcatc gtccgggaga atggactctc cgaccggaac atcgaaacct gcatcgactg
     4981 ctcccttcac tgcggcaaac acacgggcac ccgtggatgc aacctgcaga ccgatatctg
     5041 caattgctcc ctcatatccg gctttctgag cgcggactgc aaacagcata ccggtcagat
     5101 atgccgctgg ggtgttgcca aggtaaccct tgtatccata gtttaccagc tctttgctgt
     5161 tcacatgaac aagcgtgtag tcgccgtcca tctgtgcagc aacaagctga atgatgatgt
     5221 ggcggttcgt cttccggata accatacggt ttctgccgga aacaatcagc cgctggcgct
     5281 ggtagtaatc agtctttcct tcacggcgtc tgcggaactg aacaaagtat cttccattcg
     5341 tagccattgc ttagtccctc cgggaagtaa cgatctcaat ctgagccttc aggtgagcga
     5401 cgttacggaa ctgaccaccg gctgcacggc ggtagagtct gcggtattcg gatctcgtga
     5461 tggaacctgc atcacgctgt gcacgaagct ctttgcgctg tgcacggatc ttcttgatcc
     5521 actgggtctt tgacggagtg cgtgcaccct ttgcaccact gcgacgtccc ggacctttac
     5581 agtgtccata ggcacgcttg gcaatccggg cccgtgcacg tccgcgtgag atacctttct
     5641 tctggtggga ggaaattgca ccctcctcga tcagattgcg gatatcctcg cgggacattg
     5701 cactctgaac ctcggaaagt ttctctgggt tgatccagac acggtttaca ccgcagccga
     5761 gaac
//
LOCUS       DALUAJ010000046         5033 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_8261,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000046 DALUAJ010000000
VERSION     DALUAJ010000046.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8261"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(393..1001)
                     /gene="rpsB"
                     /locus_tag="O0X37_01020"
     CDS             complement(393..1001)
                     /gene="rpsB"
                     /locus_tag="O0X37_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HJK61543.1"
                     /translation="MTDNELGIELTEPLVSVEEYLAAGVHIGTQQKDDDMKEFIYRVR
                     SDGLYIIDIRKTDERIKQVAKFLARYEAPKIFVVTSRQYGQYPAQKFADTIGALSHVG
                     RFIPGTLTNPVLPKYVEPSVVVVTDPIGDSQVITEAVQCGIPVIALCDINNQTNNVDL
                     VIPTNNKGRKALSMVYFLLTREVLKQKGIVSSLTFEDFESEF"
     gene            complement(1003..2202)
                     /gene="eno"
                     /locus_tag="O0X37_01025"
     CDS             complement(1003..2202)
                     /gene="eno"
                     /locus_tag="O0X37_01025"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HJK61544.1"
                     /translation="MTTIDSITLRRILDSRGNETIEAEILTASGGYGRACAPSGASTG
                     IYEAKVRPCAEAIADAQARLIPELIDLDAQDQRTFDETLRDVDGTEDLSGIGANIAVA
                     LSLANAKAAASALNLELFQYLGGAFVDQTPLPLGNVIGGGAHAVDATDIQEFLIVPTG
                     AATAAEAVFTNARVHKTIKEILVARGKGCGKGDEGAWAPHISDAEAFEIVAEAVNKVQ
                     DATGIEVRMGLDVASSEMWNADLERYVYKNAKRTTEEQIAYIAELVDQYNLIYVEDPI
                     QEEDFEGFAQMTEEVSGRDTLICGDDLFVTNAKRLEEGITLEAGNCVLIKPNQIGTLT
                     DTFETIRLAHEYGYETVMSHRSGETTDTTIAHLGAAFNCCFLKCGVVGGERIAKLNEL
                     IRIEEHF"
     gene            complement(2204..2392)
                     /locus_tag="O0X37_01030"
     CDS             complement(2204..2392)
                     /locus_tag="O0X37_01030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit K"
                     /protein_id="HJK61545.1"
                     /translation="MIYTRYERARIIGARALQISMGAPILVRTTKIDPLEIALAEFAE
                     NMVPITVKRPIGDKVEVQ"
     gene            complement(2430..2504)
                     /locus_tag="O0X37_01035"
     tRNA            complement(2430..2504)
                     /locus_tag="O0X37_01035"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2466..2468),aa:Pro,seq:cgg)
     gene            complement(2519..2707)
                     /locus_tag="O0X37_01040"
     CDS             complement(2519..2707)
                     /locus_tag="O0X37_01040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit N"
                     /protein_id="HJK61546.1"
                     /translation="MIPVRCFTCGKVISTVWEEYQQRKAAGEDPKEILDSLGLERYCC
                     RRMLLSHKETVDELYPYT"
     gene            complement(2800..3198)
                     /locus_tag="O0X37_01045"
     CDS             complement(2800..3198)
                     /locus_tag="O0X37_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HJK61547.1"
                     /translation="MKIINTSGKRKTAIARATLKEGKGRVRINSVPLEVYGSEIIRMK
                     ISEAIALAPGALDNVDVDIDVSGGGVMGQAEAVRTALGRGILNWTNDPHIKEVYLSYD
                     RTLLVNDSRQKEAKKPHGRGARARFQKSYR"
     gene            complement(3212..3634)
                     /locus_tag="O0X37_01050"
     CDS             complement(3212..3634)
                     /locus_tag="O0X37_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HJK61548.1"
                     /translation="MVTVIDGENLLLGRMCSLVAQRVLAGEEIAIINAEKVIVSGSRA
                     MIMKEYYTKRVRGSVEGGPFYPRRPDQIVKRTVRGMIPYKTRPGAAAFRRVKAYVGVP
                     YEFKDAEAEVLEAAHRDRLSSARYVTVGAISTNLGAKY"
     gene            complement(3641..3994)
                     /locus_tag="O0X37_01055"
     CDS             complement(3641..3994)
                     /locus_tag="O0X37_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18e"
                     /protein_id="HJK61549.1"
                     /translation="MNKTNPRLESLIGMLKRASRENEANIWREIAGRLNTSSKNYAEV
                     NIGKISRYAKENEIILVPGKVLAAGVIAAPVKVAALNFSDAAREKIMEANGTCMTIEE
                     LVAANPKGSRVRILR"
     gene            complement(3995..4834)
                     /locus_tag="O0X37_01060"
     CDS             complement(3995..4834)
                     /locus_tag="O0X37_01060"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit D"
                     /protein_id="HJK61550.1"
                     /translation="MDLVFSRLDDSVARFTISGATPAFANALRRSMIGEVPTLAIEDV
                     RIYDNTSVLFDEILAHRLGLIPIKTDLSQFVPRSKCTCGGAGCPQCTVTFTLSVEGPG
                     MVTSGDLISMDPRTVPVHNNIPIVKLWEGQKIVLEATAELNTGLEHAKWQPTLACGYK
                     EFPVITIHDSCDACGKCVSECPRDVLEVADNMVHVRVVNGESKEKDCSMCRLCETACM
                     NTGIGENPAITIGADSTKFIFVVESDGSLPVKEIIERALLHIKSRSTDLTDALQDIST
                     EGL"
     gene            complement(4835..>5033)
                     /gene="rpsK"
                     /locus_tag="O0X37_01065"
     CDS             complement(4835..>5033)
                     /gene="rpsK"
                     /locus_tag="O0X37_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HJK61551.1"
                     /translation="EKGITGLHIRVRAPGNGKQRSPGPGAQAAIRALSRAGMRIGRIE
                     DVTPVPHDSIRPAGGRRGRRV"
BASE COUNT         1130 a         1334 c         1307 g         1262 t
ORIGIN      
        1 gcatcaagca gggctttcag ttcttctgct tcttttgggt aaaacattcc cgcaaggtca
       61 gcatgccgac agcctgcatc cgccgtacca gtagcagggt attcctctat tttcatggat
      121 atcaaacggg tattacgccg taaccaagga tttcgtccgc caatccgcga aagatactat
      181 tcagatattt gcggataggt gtgtgaaggt ggttatgtgt tggtttggca gggctttaat
      241 gattgcgtca caaccctgag agagtctgcg gcaccccaaa acagaacgct ggattgtacc
      301 actgcaaaag atatcctgca gacacagccc ctgtcagaaa agaaccggaa ggcagagtca
      361 gaataataca aaaaaagatg atatgagtgg atttagaact cagactcaaa gtcttcaaag
      421 gtcagggagg acacaatgcc cttctgcttt aagacctcac gggtcaggag gaagtagacc
      481 atggagagtg ccttgcgacc tttgttgttc gtcgggatca cgaggtcgac attgttggtc
      541 tggttgttga tatcacaaag ggcgataacc ggaataccgc actggacagc ctcggtgata
      601 acctgcgagt ctccgatcgg gtcagtgacc acaacaaccg acggctcgac gtacttcggg
      661 aggaccgggt tggtgagcgt accggggatg aaccggccaa cgtgggagag tgcgccgatg
      721 gtgtcagcaa acttctgtgc cgggtactgg ccgtactgac gggaggtaac aacgaagatc
      781 ttcggtgctt cgtagcgtgc aaggaacttt gctacctgct tgatacgctc gtcggtcttt
      841 ctgatatcaa tgatatacag accgtcggaa cggacgcggt agatgaattc cttcatgtca
      901 tcgtctttct gctgggtacc gatgtggaca ccggctgcca gatactcttc cacggatact
      961 aatggttcgg tcagttcaat tccaagttca ttgtcggtca ttttagaaat gctcctcaat
     1021 tcttataagt tcatttagtt tagcaatacg ctcaccgcca acaacaccgc acttgaggaa
     1081 gcagcagttg aatgcagctc caaggtgtgc aattgtggtg tcggttgtct caccggaccg
     1141 gtggctcatg acggtctcat acccatactc atgggcaaga cggatcgtct caaacgtatc
     1201 ggtcagggtt ccgatctggt taggcttgat taagacacag ttgcctgctt caagggtgat
     1261 tccttcctcc agacgctttg cgttggtaac aaagagatca tctccgcaga tgagtgtgtc
     1321 acggccggag acctcttcgg tcatctgtgc gaagccttca aagtcctcct cctgaatcgg
     1381 gtcctcaaca tagatcaggt tgtactgatc caccagctcg gcgatgtatg cgatctgctc
     1441 ctcagtagtg cgctttgcat tcttatacac ataccgctca agatcagcat tccacatctc
     1501 ggaagatgca acgtcaagac ccatgcggac ctcaattcct gttgcatcct gcactttgtt
     1561 taccgcttcc gcaacgatct cgaatgcttc tgcatcgctg atgtgcggag cccaggcacc
     1621 ctcgtcaccc ttgccgcagc ctttaccgcg cgcaacaaga atctctttga tggtcttgtg
     1681 cacacgggcg tttgtaaaga ctgcctctgc ggcggttgca gcacctgtcg gaacgatcag
     1741 gaactcctga atatcggttg catccaccgc atgggcaccg ccgccgatca cattgccgag
     1801 cggaagcggg gtctgatcca caaatgctcc gccaaggtac tggaacagct caaggttcag
     1861 ggcagatgca gctgcctttg cgtttgcaag agagagtgca actgcgatgt ttgcaccaat
     1921 gccggagaga tcctcggtac catctacatc gcgcagcgtc tcatcaaagg tacgctgatc
     1981 ctgcgcatca agatcaatca gttccggaat cagccgtgcc tgtgcatcag caattgcttc
     2041 agcacatggc cgcacttttg cttcatagat acctgtcgat gcaccgcttg gtgcgcatgc
     2101 acggccgtat cctccgcttg cagtcaggat ctccgcttca atggtctcat ttccacggct
     2161 gtcaagaatt ctccggagcg taatgctgtc aattgtggtc atattactga acctctactt
     2221 tgtcaccaat ggggcgctta acggtgatag gaaccatatt ctctgcaaac tcggcaaggg
     2281 cgatctcaag cggatcgatc ttagtagttc tgacaagaat aggggcaccc atcgagatct
     2341 gtaaagcgcg cgcaccgatg atcctggccc gttcataacg agtgtatatc attggcaatc
     2401 acatccataa aacataaaga atggaaaaat ggggtcgctg agattcgaac tcaggtcact
     2461 acgtcccgaa cgtaatagga tggtccaggc taccctacga ccccgcatca ttcatgcatc
     2521 acgtgtaggg atacagctca tcaacagtct ctttgtgcga gagaagcatg cgtctgcaac
     2581 aataccgctc aagcccgaga gagtcgagaa tttctttcgg atcttcaccg gcagctttcc
     2641 gctgttgata ttcttcccat accgtggaaa tcactttccc acaggtgaaa catcgaactg
     2701 gtatcattag ttatccttct aaggttatta atctgttgtt ttgcaaatgg gggtgaggat
     2761 atcatcccct ttgcagaacc catattctga aagctagatt taacggtacg acttctggaa
     2821 ccgtgcacgt gcaccgcgtc catgcggctt cttggcttcc ttctgacggg agtcattgac
     2881 aagaagcgta cggtcgtagc tgaggtaaac ctctttgatg tgcgggtcgt tggtccagtt
     2941 caggatgccg cggccgagtg ccgtgcggac agcctctgcc tggcccatga cgcctccgcc
     3001 ggagacatcg atgtcaacat caacgttgtc gagtgcaccc ggtgcgagag cgattgcctc
     3061 ggagatcttc atgcggatga tttcgcttcc gtagacctcg agcgggacag agttgatacg
     3121 gactctgccc ttaccctctt tgagggttgc gcgtgcaatt gccgtctttc tcttaccact
     3181 ggtgttaatg atcttcatct ttctccacct cttagtactt tgctccaagg ttggtgctga
     3241 ttgcgccaac ggtgacgtaa cgtgcgctgc ttaagcggtc gcggtgtgcg gcctcaagaa
     3301 cctctgcctc tgcgtcctta aactcgtaag gaacaccaac atatgccttg acacggcgga
     3361 aagctgctgc tcccggccgg gtcttgtacg ggatcatgcc gcggacggta cgcttgacga
     3421 tctggtcagg gcgtctcggg tagaacggac cgccttcaac ggaaccacga acacgcttgg
     3481 tatagtactc tttcatgatc atcgcacggg aaccggagac gatgactttc tcggcgttga
     3541 taatagcgat ctcttcgcct gcaagaacac gctgggcaac aaggctgcac attcttccga
     3601 gcagaagatt ctctccatca ataactgtta ccatttcagt tcacctcagg atccggacgc
     3661 ggctgccctt cgggtttgct gcgacaagct cctcgattgt catgcaggta ccgttggctt
     3721 ccatgatttt ttcacgcgca gcatcggaga agttaagtgc ggcaaccttg acgggtgcag
     3781 cgatcacacc tgctgccagg actttacccg gaaccaggat gatctcattc tcttttgcgt
     3841 agcggctaat ctttccgatg ttaacttcag cgtagttctt gctggacgtg ttcaggcgtc
     3901 ctgcaatctc acgccagata ttagcctcgt tttcccggga tgcccgcttg agcataccga
     3961 ttaaggattc gaggcggggg tttgtcttat tcatttacag tccctccgtg gatatgtcct
     4021 gtaatgcatc agtcaggtct gtggatcgtg acttgatatg cagcagtgct ctttcgatga
     4081 tctctttgac cggaagggac ccgtcgcttt cgacaacgaa gatgaacttc gtactgtcgg
     4141 caccgatggt gattgcgggg ttttcaccaa ttccggtgtt catacatgcg gtctcacaga
     4201 gacggcacat tgaacagtct ttttctttgc tttcgccgtt tactacgcgg acgtgaacca
     4261 tgttgtcggc cacttcaaga acgtcgcgcg gacattcgga aacacatttc ccacaggcat
     4321 cacagctgtc gtggatcgtg attaccggga actctttgta accgcaggca agagtcggct
     4381 gccatttggc atgctcaaga ccggtgttga gttcggcggt ggcttccaga acaatcttct
     4441 gaccttccca gagcttcaca attggaatgt tgttgtgcac agggactgtg cgcgggtcca
     4501 tggagatcag atcaccggag gtgaccatgc ccggaccttc aacactcaga gtgaacgtaa
     4561 ccgtgcactg cggacagcct gcaccaccac aggtacattt gctacgcgga acaaactggg
     4621 acaggtcggt tttaatcgga ataagtccaa gtctgtgtgc aaggatctcg tcaaagagaa
     4681 cgctggtgtt gtcatagatg cgaacgtctt cgatggcaag ggttggcacc tcgccgatca
     4741 tagagcgtct gagggcgttt gcaaatgccg gagtcgctcc gctgatagta aaccgtgcga
     4801 cagagtcatc gagcctgctg aatacaagat ccattcagac tctcctgcct cttctaccac
     4861 ccgctgggcg gatgctgtcg tgcggtaccg gggttacatc ttcgattctg ccgatacgca
     4921 tacctgcacg ggaaagcgca cggattgcgg cctgggcacc cggtcccgga gagcgctgct
     4981 tgccgtttcc tggtgcacgg acacgaatgt gaagtccggt gattcccttt tcc
//
LOCUS       DALUAJ010000047         5870 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_8265,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000047 DALUAJ010000000
VERSION     DALUAJ010000047.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8265"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="O0X37_01070"
     CDS             <1..868
                     /locus_tag="O0X37_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HJK61552.1"
                     /translation="AYSTIANLGLITACAGIGTPEAVMAAILLIIFHAVAKALLFMCV
                     GAVEHKIDSRDIEDMDGLLVRLPLLATMMVIGICGMYLAPFGMLISKWAAIVAFLEAP
                     FGWAFVILLAFGSAFTIFFWTKWLGKLFMRMNHPQEPDTMLHTSEKIAVTATGVFTIL
                     CCIGFAAITVYFVEPYLADIYAGFSETGAMLPDNILMIAIMFVILAVLVLAAHFGSVS
                     GKTLPPYLCGRGVDENGRFHGSLGVYKEAKSSNYYMEEYCGEAILIKPAWIISILLII
                     VMFVLAFLGVMI"
     gene            865..1737
                     /locus_tag="O0X37_01075"
     CDS             865..1737
                     /locus_tag="O0X37_01075"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HJK61553.1"
                     /translation="MNLILAATGAVLFLILAPLVGGLIAGVDRVLTARMQSRRGPPVL
                     QPFYDISKLWHKEPAFVNPVEKIFTTAYLVLIAFSGALLFAGGDLLLVVFALALAHTF
                     LILAAYAANAPYSTVGAERELIGVMVAEPVLILLAAGFFMVTGSFFVSDILKEEIPAI
                     IYLPGVFLALFAVLTLKLRKSPFDISTSHHAHQELVKGVTSSLSGKTLAKVEIAHWYE
                     TILILALIFLFFAGNPILGIIVVIICYFLEILMDNLFPRVTWQMTVKSLWIATVILGV
                     INIAVIGFVGGVIG"
     gene            1734..2174
                     /locus_tag="O0X37_01080"
     CDS             1734..2174
                     /locus_tag="O0X37_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HJK61554.1"
                     /translation="MSSAGKSPWLLHYNASSCNGCDIEILAALTPLYDVERFGIINTG
                     NPAHADIFVVTGSVNEQNKEVLKNLYDQIPEPKVVVAVGICASSGGVFRDCYNVSGGV
                     DTVIPVDVYVPGCSVRPEALIEGVVKAIGVLEQKRAVLDSGGSL"
     gene            2171..2554
                     /locus_tag="O0X37_01085"
     CDS             2171..2554
                     /locus_tag="O0X37_01085"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HJK61555.1"
                     /translation="MTMQIIPASPAGIEKIAADMKAEGRRLVVITCTAAAEEYDITYS
                     FDKENDLVHYRLTVPEGTVIPSIGASYGGAFVYENEIHDLYGFTFTGMTLDFGGTFIR
                     TKIQYPFKKEKPAPTVTKVTKEGAS"
     gene            2551..3633
                     /locus_tag="O0X37_01090"
     CDS             2551..3633
                     /locus_tag="O0X37_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HJK61556.1"
                     /translation="MTGKRTVVPFGPQHPVLPEPIHLDLVIEDEHVVEAIPSIGYVHR
                     GLESLVDRREYADFVYLAERICGICSFTHSSTFCQGVEGLMGIEIPERANYLRTMWGE
                     YSRIHSHLLWLGLFADALGFESLFYNAWKIRESILNELEATTGGRVIQGVCKVGGVRR
                     DVTNEFLSGMDKRLEGIEAEMQEMVNVFLNDYTMKKRLIGKGILKPEDAYNLGAVGPV
                     ARGSGLAQDVRTSEYLAYQDIGFKPVTANGCDGYARCEVRCKELFQSAEIIHKVIAAM
                     PDGEVAVPVKGNPDGEYFSRSEQPRGEVIHYLRGNGTKYLTQFRVRTPTLTNVPSLVA
                     MLAGAELADVPQIVLTIDPCIGCMER"
     gene            3639..4019
                     /locus_tag="O0X37_01095"
     CDS             3639..4019
                     /locus_tag="O0X37_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK61557.1"
                     /translation="MKMLKTILKQVTHKPVTTTFPAEPAKHFDATRGHIVFDPSKCRS
                     CTICMKRCPSQAIVVDRAKNTWTIDRFRCIICSQCVELCKFGGLSLATEYSACATPEE
                     RGTETFAITYVKPERPKKDAECGE"
     gene            4186..4953
                     /locus_tag="O0X37_01100"
     CDS             4186..4953
                     /locus_tag="O0X37_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HJK61558.1"
                     /translation="MGERYWEVDAIRGIALIGMIVYHLLACMVIFHMIVEDEEFLTYY
                     GTINIASASFVLIAGVALILRHARKKGRTTQEYYRSIIIKALFLFIVAIGITIVTKIG
                     AELILHNDVYVKFGFLHMLSISILLSLPFLRFGKWNILPGIIIVLIGILLIPQITAPE
                     WLYPLGIHGAEFTDTTQDYFPLFPWFGVLLIGIALGDIFYPNGIRSFRFPNAGKVGKF
                     FSRIGNGKVTLAVYLAHIPIIFVVLWIISQITGIGYI"
     gene            5022..>5870
                     /locus_tag="O0X37_01105"
     CDS             5022..>5870
                     /locus_tag="O0X37_01105"
                     /inference="COORDINATES: protein motif:HMM:NF016312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HJK61559.1"
                     /translation="MQIDIIHSTVDTAGVNLRAAIDDLIQHPPEGGWPLLTKHQITFR
                     TWNERIINASDAITSPDADLVIFLARHSSVNPVPVLTVHPAGNFTTADLGGSPRELGL
                     AAPAWMRAILRNHQKYAPEGFRVSYEITHHGPTNIHVPYFFVEVGSTEAEWQNMEAIR
                     AAATSVLMADPSEEIIPLIGFGGTHYAVRQTAISLETRGAFGHMMHTRNIAGADTEII
                     RQMIVKSGAIAAHVDKKAMSKSEAAHIEGLLKEIGIPEITEGDLHKIGTMPWKGWTEY
                     LSFAKEI"
BASE COUNT         1521 a         1417 c         1473 g         1459 t
ORIGIN      
        1 cgcatactct acaattgcaa acctcggcct gatcactgct tgtgccggaa tcggaacacc
       61 agaggctgtc atggcagcga ttctcctgat aatcttccac gcggttgcaa aagcactcct
      121 gtttatgtgt gtcggtgcag ttgaacacaa aatcgacagc cgtgacattg aagacatgga
      181 cggactgctt gtacggctcc cgctgctggc aaccatgatg gtcatcggta tctgcggcat
      241 gtatcttgca ccattcggta tgctcatctc caaatgggca gcgattgttg cgttcctgga
      301 agcgccgttc ggctgggcat ttgtcatcct ccttgcattc ggcagtgcat ttacaatctt
      361 cttttggacg aagtggcttg gaaaactgtt catgcgcatg aaccacccgc aggaaccgga
      421 taccatgctg cacacatctg aaaagattgc ggtaactgcc acaggagtct ttacaattct
      481 ctgctgcatc ggctttgctg caattacagt gtactttgtc gagccatatc tcgcagatat
      541 ctatgcagga ttttcagaaa ccggagcaat gcttcccgat aatattctca tgattgctat
      601 catgtttgtg atccttgcag tcctcgtact tgcagcacac tttggatctg tttccggtaa
      661 aacactcccg ccgtaccttt gcggtcgcgg agttgatgag aacggaagat tccatggatc
      721 acttggtgtg tacaaagaag caaaatcctc gaactactat atggaagagt actgtgggga
      781 agccatactg atcaaacctg cgtggatcat ctccattctc ctcatcattg tcatgtttgt
      841 actcgccttt ctgggggtga tgatatgaat ctgatacttg cagcaaccgg tgcggttctg
      901 ttcctgattc ttgctccgct agtcggcgga ctgattgcag gagttgaccg cgtacttaca
      961 gcacggatgc agagccggcg cggccctccg gttttgcagc cgttctatga tatcagcaaa
     1021 ctctggcaca aagaacctgc attcgtaaat ccggtggaaa aaatcttcac cacagcctat
     1081 ctggtactga ttgcgttctc cggcgcactg ctgtttgcgg gcggtgatct gctgctggtc
     1141 gtctttgccc tggcactcgc acacacgttc ctgattcttg cagcgtatgc ggcaaacgcc
     1201 ccctactcaa cagtgggcgc tgagcgtgaa ctgatcggtg taatggttgc agagccggtg
     1261 ttgatcctcc ttgccgcagg atttttcatg gtgacaggca gcttctttgt ctcagacatt
     1321 ctcaaagaag aaattccggc aatcatctat ctgccaggtg tcttcctcgc tctctttgca
     1381 gtactgacac tgaaactgcg caagtcaccg ttcgacatct ccacatctca ccacgcccac
     1441 caggagctgg taaaaggtgt gacctcgtca ctttctggaa aaacgcttgc aaaagtggag
     1501 attgcgcact ggtacgagac gattctgatt cttgcactga tatttttgtt ctttgcagga
     1561 aatccgattc ttggaattat cgtagttatc atctgctact tccttgaaat actcatggac
     1621 aacctcttcc cgcgggtaac atggcagatg acagtgaaga gtctgtggat tgcaacagtt
     1681 atccttggtg tgattaacat tgcagtgatc ggatttgttg gaggtgtgat tggatgagct
     1741 cagcaggaaa gtccccctgg cttctgcact acaacgcgtc cagctgtaac ggatgcgata
     1801 ttgaaatcct tgcagcactg acgccgttgt acgatgtgga acggttcggc attatcaata
     1861 ccggtaaccc tgcccacgca gatatttttg tggtgactgg cagtgtgaac gagcagaaca
     1921 aagaggtgct gaaaaacctc tacgatcaga taccagaacc aaaagttgtt gttgcagtag
     1981 gaatctgtgc ttcgtccgga ggcgtgttcc gcgactgtta caacgtttcc ggcggtgttg
     2041 atacggtcat tccggtggat gtatatgtac cgggctgctc ggttcggcct gaagcactca
     2101 ttgaaggagt ggtcaaagca ataggtgttc ttgaacaaaa acgtgctgtg ctcgacagcg
     2161 gaggatcact atgacgatgc aaattatccc tgcatcccct gcaggaattg aaaaaatcgc
     2221 ggctgatatg aaagccgaag ggcgccgtct tgtggtgatt acctgcactg ctgcggccga
     2281 agagtatgat atcacctact cgtttgacaa ggaaaacgat ctggtacact accgtctcac
     2341 agttccggag ggaacagtga ttccatcaat aggtgcatca tatgggggag catttgtgta
     2401 cgaaaacgag attcatgatc tctacggatt tacgttcacc ggaatgacgc ttgattttgg
     2461 aggaacattc atccgtacaa agatacagta tccgtttaag aaggaaaaac cagcaccaac
     2521 ggtgacaaaa gttacgaagg agggagcctc atgacaggaa aacgaactgt tgtaccgttc
     2581 gggccgcagc atccggtact gcccgaaccg attcaccttg atctcgtaat cgaggatgag
     2641 catgtggtcg aggcaatccc gtccatagga tatgtacacc gtgggctgga gagcctcgtg
     2701 gaccgccgcg agtatgcaga cttcgtctat cttgcagaac gtatctgcgg tatctgcagc
     2761 ttcacccact cctccacgtt ctgtcagggt gtagagggac tgatgggtat tgagattcca
     2821 gagcgtgcca actatctcag gacaatgtgg ggggaatact cgcgtattca cagccacctg
     2881 ctctggttag gcctcttcgc agatgcactt ggatttgaga gtctcttcta caatgcatgg
     2941 aagattcgcg aatcgatctt aaacgagctg gaggcaacca ccggcgggcg ggtaattcag
     3001 ggagtctgca aagtgggagg tgtccgccga gatgtgacaa atgagttcct gtccggaatg
     3061 gacaaacgac tcgagggtat tgaggcggag atgcaggaaa tggtaaatgt attcctgaac
     3121 gactacacaa tgaagaaacg gctgatcggg aaagggattc taaaaccaga agacgcatac
     3181 aatcttggag cggtcgggcc ggtcgcacgg ggatcaggcc ttgcacagga cgtccggaca
     3241 tccgaatatc tggcgtatca ggacatcgga tttaagccgg ttactgcaaa cggatgtgac
     3301 ggatatgctc gttgcgaagt aaggtgcaaa gaactgttcc agtccgcaga gatcatccac
     3361 aaagtgattg ccgcaatgcc ggatggcgag gtcgcagtac cggtgaaagg taatccggat
     3421 ggggagtact tcagccgttc agaacagcca cgcggcgaag tgatacatta cctccgtgga
     3481 aacggtacaa agtacctgac acagttccgt gtgcggacac cgaccctgac aaacgttccg
     3541 tcactggtcg ccatgcttgc cggggcagaa cttgcggatg tcccgcagat tgtactgaca
     3601 atcgatccgt gcatcgggtg catggagagg tgagactgat gaagatgctg aaaaccatcc
     3661 tgaagcaggt tacacataaa ccggtgacaa caacatttcc ggcagagcct gcaaaacatt
     3721 ttgatgcaac ccgtggccat attgtattcg atccgtcaaa gtgccggtca tgtacaatct
     3781 gtatgaaacg ctgcccgtca caggcgattg tcgtggaccg tgcgaaaaac acatggacga
     3841 ttgataggtt ccggtgcatc atctgtagcc agtgcgtgga gttgtgtaaa ttcggaggac
     3901 tgtcacttgc aacagagtac tcagcctgtg caacaccaga ggaacgcggg acagaaacgt
     3961 ttgctatcac atacgtaaaa ccggagcggc ctaaaaaaga tgctgaatgt ggagaataag
     4021 tattcctgat ccatcaaaat gaactgatgt tactcagcat cattgtatat ttttcccaca
     4081 tcaaatttca aaaatatttt ttctgatctg acatctaaca atggatggag aacttcgtca
     4141 atatacaaaa tatataatga tcaaaaccaa ataggacggg agactatggg agaaagatac
     4201 tgggaggtgg atgcaatccg gggtattgcc ctcatcggga tgattgtata ccatcttctt
     4261 gcctgtatgg taatcttcca tatgattgtt gaggacgagg agtttctcac ctattacggg
     4321 acgatcaaca ttgcatcagc atcattcgtc ctcatcgcag gagtggcact gattctgcgg
     4381 cacgccagaa aaaaagggag aacaacccaa gagtactacc gttccatcat catcaaagcg
     4441 ctgtttctgt tcattgttgc aataggtata acaatagtca ccaagattgg tgcagaactt
     4501 atcctccata atgatgtgta tgtgaagttt gggttcctgc atatgctaag tatctcaatc
     4561 cttttgtccc tgccgttttt gcggtttggg aagtggaata tacttccagg aattatcatc
     4621 gttctcatcg gtatcttgct gatcccccag attaccgcac cggaatggct gtatccgctg
     4681 gggatccacg gcgcagagtt taccgataca acgcaggact acttcccgct tttcccctgg
     4741 tttggtgttt tgctgatagg tattgcactc ggtgatatct tctatccaaa cggaatacgc
     4801 agtttccggt ttccaaacgc agggaaagtt ggaaaatttt tttctcgcat cgggaacggc
     4861 aaagtaacac tggccgtgta tctggcacat attccgataa tatttgtggt actgtggatc
     4921 atcagccaga tcacgggtat cggctatatc tgagtacatt tttttgaaac agagtccagg
     4981 gagaaacaca catcatttcc cacaccctaa actcttacca gatgcagatc gatatcatcc
     5041 actccaccgt tgatactgcc ggagtaaacc tccgtgccgc aatcgacgat ctcattcaac
     5101 atccgcctga aggtggatgg ccgctcctca ccaaacatca gataaccttc cgtacgtgga
     5161 acgaacgcat catcaacgca agcgatgcaa tcaccagccc agacgcagac ctcgttatct
     5221 ttcttgcacg acactcaagc gtcaacccag tcccggtatt aaccgttcat ccagcaggaa
     5281 acttcaccac tgcagatctc gggggcagtc cccgcgaact tggtcttgca gcccctgcat
     5341 ggatgcgtgc tatactgcgc aaccaccaga aatatgcacc tgaggggttc cgagtgtcgt
     5401 acgaaattac acatcacggt ccaaccaata tccatgtacc ctatttcttt gtagaggtcg
     5461 gcagcacaga agcagaatgg caaaacatgg aagctatccg tgcagctgca accagtgtcc
     5521 tcatggcaga tccaagtgaa gagatcattc ccctgatcgg atttggcgga acgcattatg
     5581 ccgtcaggca aacagcaatt tctttggaaa cacgcggagc attcggccac atgatgcaca
     5641 cgcgaaacat tgcaggtgca gatactgaaa ttatccggca gatgattgta aaaagcggtg
     5701 ccatcgccgc gcacgttgat aaaaaagcga tgtcaaaatc agaagccgca cacattgaag
     5761 ggttgctgaa agagatagga attccagaaa taacagaagg agatctgcat aagattggga
     5821 ccatgccatg gaaaggatgg acagagtatc tttcctttgc aaaagagatc
//
LOCUS       DALUAJ010000048         3070 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_8460,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000048 DALUAJ010000000
VERSION     DALUAJ010000048.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8460"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1712)
                     /locus_tag="O0X37_01110"
     CDS             complement(<1..1712)
                     /locus_tag="O0X37_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK61560.1"
                     /translation="MNRIVERLSGSIREYRRDSILAPVFVALEVVMEVLIPLVLADLI
                     DDGITAGNMDIIIKLGLVLLLFAIMSLVFGVFAGKYAASASAGFAKNLRHDLFYAVQN
                     FSFANIDKYSTASLITRMTTDVTNVQNAYQMIVRIAIRCPLMLIFAFLMVVGIHAQLA
                     LIFLVLLPILGIGMYLIITRAQPLFELVFKTYDSLNKVVRENLRGIRVVKSYVREDYE
                     IKKFRSVSGSIYGYFSRAEKILAFTSPLMQAVVYASILLISWFGAQFIISGSLTRGDL
                     ISLISYSMQILMSLMMLAMVFVMITMAQASSRRIAEVLEEEVDLTNPSDPVFTVADGS
                     VEFRDVGFQYTKDAEQEILKDINLKIRSGETVGILGGTGSAKSTLVQLIPRLYDTTKG
                     EVLVGGVNVREYNLTTLRDAVAMVLQKNVLFSGTIRDNLKWGNPDATDEEIARACRLA
                     KADEFIETLPEGFDTKVEQGGANFSGGQKQRLCIARALLKKPKILILDDSTSAVDTRT
                     DALIRQAFRTEIPDTTKIIIAQRIASVQDADNILVMDAGRICARGTHEELLQNNAIYR
                     EMYRS"
     gene            complement(1709..2062)
                     /locus_tag="O0X37_01115"
     CDS             complement(1709..2062)
                     /locus_tag="O0X37_01115"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HJK61561.1"
                     /translation="MTGIHGWIIGHLCRHPERDIFQKDIEAILSIRRSTATGILQLQL
                     MEKNGLIIREPVPYDARLKKLVPTQKALAVNRRLEEEFKRINAQLIHGLTKEELDIFC
                     SVAEKMQANLNGGES"
     gene            2283..>3070
                     /locus_tag="O0X37_01120"
     CDS             2283..>3070
                     /locus_tag="O0X37_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HJK61562.1"
                     /translation="MYCQQCQESKNPACTLGGACGKNKTVAEAQDRVLYSLSCLAFRA
                     KSDSTDLQPCVLEALFATLTNVSFDEARFDEYLSCITGHYNSLPPAAGEPAFDADAGL
                     DAISDTNTRSLFSLLLFGVKGLAAYYSHALVLGKADDTILPFIRDALASRFEDLSIDQ
                     RTALVLECGKQGVSVLALLDAANLTYGVPEITKVSTSSGVRPGILVTGHDLKDLAMLL
                     EATKDAGVDVYTHGEMLPAHAYPAFKKYPHLRGNFGGSWAEQRS"
BASE COUNT          726 a          753 c          730 g          861 t
ORIGIN      
        1 gaccggtaca tctctcggta tatggcattg ttttgtaaca gttcctcatg tgtccccctg
       61 gcacagatac ggccggcatc cataaccagg atattgtctg catcctgtac cgacgcaatt
      121 ctttgagcga tgatgatctt tgttgtgtcg ggaatttctg ttctgaatgc ctgccggatc
      181 aaagcgtctg ttcttgtatc gactgcactt gttgagtcat ccagaatgag aatttttggt
      241 tttttcagta gtgcccgtgc aatacaaagc ctctgtttct ggcctccgga gaagtttgcc
      301 cctccctgtt ccacctttgt gtcgaaccct tccggtagtg tttcaatgaa ctcgtccgcc
      361 tttgcaagtc tgcatgcacg ggcaatttct tcatcggttg cgtcgggatt tccccatttg
      421 agattatcac gaatggttcc ggagaacagt acattctttt gtaaaaccat tgcaaccgca
      481 tcccgcaggg tggtgagatt atactcccgt acgtttactc ctccgacgag aacctcacct
      541 tttgttgtat catataaccg gggaatcagc tgtaccaggg tcgactttgc actcccggta
      601 cctccgagaa ttccaactgt ttctccagat cggattttca gattaatgtc tttgaggatc
      661 tcctgttcgg catcttttgt gtactggaat ccaacatcac ggaactccac gctcccgtct
      721 gcaactgtga aaaccggatc tgatgggttg gttaagtcca cctcttcttc aagtacctcg
      781 gcaatcctcc gggatgacgc ctgtgccatt gtgatcatga caaaaaccat ggcaagcatc
      841 atgagactca tcagaatctg catagagtat gagataagac ttatcaggtc gccccgtgtc
      901 aggcttccag aaatgatgaa ctgtgcccca aaccaggata tgagaagaat acttgcatag
      961 acaactgcct gcatcagagg actcgtaaat gcaaggatct tttctgcacg cgagaagtac
     1021 ccgtagatgg atcctgagac gctgcggaac tttttgatct cataatcttc ccgtacatag
     1081 gactttacca cacggatccc gcgaaggttt tcccgcacca ctttgttgag tgaatcatag
     1141 gtcttaaaca ccagttcaaa cagcggctgt gcacgtgtga tgatcagata catcccgata
     1201 ccaaggattg gcagaagtac aaggaagatc agtgccagct gtgcgtgaat tccaaccacc
     1261 atcaggaacg caaagatcag catcagcgga cagcggatgg ctatcctaac gatcatctga
     1321 taggcattct ggacgttggt gacatcagtt gtcatacggg tgatgagact tgctgttgag
     1381 tacttgtcga tgtttgcaaa cgaaaagttc tgaactgcgt agaagagatc atgccggaga
     1441 ttttttgcaa atcctgcaga cgcggatgct gcgtactttc ctgcaaagac accgaagacg
     1501 agtgacatta tcgcaaacag cagcagaaca agtccgagct ttatgatgat gtccatgttg
     1561 ccggcggtga ttccgtcatc aatgagatcc gcgagaacta gaggtatcag aacctccata
     1621 acgacctcta gtgcgacaaa taccggggca agaatactgt cacgtcggta ctcgcggata
     1681 gatccggata gtcgttcaac gattcggttc acgattctcc tccattgaga tttgcctgca
     1741 tcttttcggc aaccgaacag aagatatcca gttcttcctt ggtaagaccg tgtatcagtt
     1801 gtgcgttaat ccgtttgaat tcttcttcga ggcggcggtt tactgccagt gctttctgtg
     1861 ttggaaccag tttcttgagc cttgcatcat atggtaccgg ttcacgaatg atgaggccgt
     1921 ttttttccat aagttgaagt tggagtattc ccgttgctgt tgatcgccgg atggagagaa
     1981 ttgcttcaat gtctttctga aatatgtcac gttcggggtg tcggcaaagg tggcctatga
     2041 tccacccgtg tatcccggtc ataccactgc agtcgcagaa acagtcggtg ttctgtagtg
     2101 ttcgtctgat ggtatttgta agcgatttta tctgaatgat gaccggttgt tccatacgtt
     2161 atttttgtta ggaagctaac aaataatgtc ttttccccat ggtatggttt tggaaacttt
     2221 ttcctatatc tctgagtatg ggcaatatac ttactatcca aaagaaactt tggtgataat
     2281 ccatgtactg ccaacaatgt caggaatcaa aaaaccccgc ctgtaccctt ggtggagcct
     2341 gtggaaaaaa caaaaccgtt gcggaggcac aggaccgtgt gctctactca ctctcctgtc
     2401 ttgcattccg tgcaaaatcc gacagcactg atctgcaacc gtgcgttctg gaagcactgt
     2461 ttgcaacatt gaccaatgtc agcttcgacg aagcacggtt tgacgaatat ctctcctgca
     2521 tcaccggtca ctacaacagt ctgcctccgg cagcaggcga acctgctttt gatgcggatg
     2581 caggtcttga tgctatcagt gataccaaca cccgttccct cttctcgctg ttgctctttg
     2641 gtgtgaaagg ccttgccgcc tactactcgc acgcacttgt ccttggaaaa gccgatgata
     2701 ctattcttcc gtttatccgg gatgcgcttg cttcgcggtt tgaggatctc tccatcgatc
     2761 agcgcactgc acttgttctt gagtgtggta aacagggtgt ttcggtcctt gcacttcttg
     2821 atgcagcgaa cctgacctac ggggtcccgg agataacaaa ggtctctaca agttctgggg
     2881 ttcgccccgg tattttagta accggtcatg atctcaagga tcttgctatg cttcttgagg
     2941 caacgaagga tgcaggagtt gacgtttaca cccacggtga aatgctgcca gcccatgcat
     3001 acccggcgtt caagaagtat ccgcatcttc gcggaaactt tggtggttcg tgggctgagc
     3061 agagatcaga
//
LOCUS       DALUAJ010000049         3421 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_8727,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000049 DALUAJ010000000
VERSION     DALUAJ010000049.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8727"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1908)
                     /locus_tag="O0X37_01125"
     CDS             complement(<1..1908)
                     /locus_tag="O0X37_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PINc/VapC family ATPase"
                     /protein_id="HJK61563.1"
                     /translation="MILVPDTSVVIDGRVTALIEQGEYRGATIIIPEAVFAELEAQAN
                     QGREIGFSGLSELQKLCKMKEEGLINLEYVGVRPKLEQVKLAGGGEIDSMIREVAIAH
                     NATFLTSDYVQAEVARAKGINVVFLRPEITDNFAPLLIDAFFDENTFAVYLKERVTPY
                     ARKGTIRESKLVTIRDTPATEYELRAIAQECLERAKRHPDGFVEVEMPGVTVSQIGSM
                     RITTTRPPFSDGIEITIVRPTREVSFDSYNYAVALKERLNIGNGGMLIVGTPGSGKST
                     LEQNIATYLSSENYIVKTIESPRDLMVPDKITQYTALDGSIAATGEVLTLLRPDYVVF
                     DEMRRSEELAVFADLRLSGIKVIGGLHARSAPDALIRLASLVDLNLIPQIVATVVFVK
                     DGDISDLYNVSTVFGVPAALERIGDPQVRPIVRFTNIITGVVAAEAFRYEGEVTVTSG
                     GGIHPAEQTETPAATVQVINATPAEVIETTATSVTESTSEVNGDGDDASHVPPPIHSM
                     PTYAVTEDNPAWIILEKEIQKEISRFTEGEIIVRMINDTKAAVYIEDADVPAAIGKAG
                     KNIAAVVSKVGVGIDVRPLSDLPGREVSTPAVPAGSGSSGAFKIRSEKKQLALICPEY
                     SNKIVDLFSGKE"
     gene            complement(1953..2330)
                     /gene="hisI"
                     /locus_tag="O0X37_01130"
     CDS             complement(1953..2330)
                     /gene="hisI"
                     /locus_tag="O0X37_01130"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HJK61564.1"
                     /translation="MTDVLDNLTYDGGLIPVIVQDIATKTVLMFAYANKEAVTLTKET
                     GFAHYYSRSRQKLWKKGEESGHLQKVCEIRVDCDHDCLLYLVEQTGCACHEGYFSCFF
                     RTIDGRIILPRLKDPQEIYHKIQ"
     gene            2482..2889
                     /gene="cfbA"
                     /locus_tag="O0X37_01135"
     CDS             2482..2889
                     /gene="cfbA"
                     /locus_tag="O0X37_01135"
                     /EC_number="4.99.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin nickelochelatase"
                     /protein_id="HJK61565.1"
                     /translation="MSKNGLLLVGHGSRLQYNKELITTTAQMMAERTDKYLIKSCFME
                     NSVPTVPEGLDAMRKEDLDLLVVVPLFLAKGIHVLRDIPGLLGLESGSNRGVFTLENG
                     REIPVVYAEPIGIDPLLAELMLKNAEKAVTTYL"
     gene            2897..>3421
                     /locus_tag="O0X37_01140"
     CDS             2897..>3421
                     /locus_tag="O0X37_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F430 synthase"
                     /protein_id="HJK61566.1"
                     /translation="MHVLVLDTIHGGEDIAEALRQRGDTVDAVDVYRGTGVPESVAAS
                     RRYDLVTAPVHLIPSHPLLAKSPVKTHHEMVRELVVPPRISVEITGARGKTTTAFALA
                     HLMTDLGCGILHTSSGTFRMPDRELLWRKSITPASVIAASASAQKFGAEWLIAEESVG
                     VAGFGTLGILTSGDD"
BASE COUNT          766 a          808 c          882 g          965 t
ORIGIN      
        1 ctcctttcct gagaagagat caacaatctt gttgctgtac tctgggcaga ttagggcaag
       61 ctgttttttc tcacttctga tcttgaatgc gccggaggat cctgatcctg ccggtactgc
      121 cggagtagag acctcccgtc ccggaagatc gcttagcgga cgaacgtcaa tgccaactcc
      181 aactttactg acaactgcgg cgatgttctt tcctgctttt ccgattgctg cagggacatc
      241 tgcgtcttca atatagacag ctgccttggt gtcgttgatc attctgacga taatctcgcc
      301 ttcggtgaat cgggagatct ctttctgaat ctccttttcg aggatgatcc atgcggggtt
      361 gtcttccgtg acagcatagg ttggcatact gtgaattggc ggcggaacat gtgatgcgtc
      421 atcaccatcg ccatttacct cagatgtgga ttctgttacc gatgttgcag ttgtctcaat
      481 aacttcagca ggcgttgcgt tgattacctg cactgttgca gccggtgtct cggtctgttc
      541 tgctgggtgg attcctccgc cggaggtaac agttacttct ccttcgtaac ggaatgcttc
      601 agctgcaaca acgcctgtga tgatgtttgt gaagcggaca atgggtctga cctgcgggtc
      661 gccaatccgc tccagtgctg cgggtactcc aaagacagta ctgacattgt acaaatcgga
      721 gatgtcacca tccttgacaa agaccaccgt tgccacgatc tgcgggatga ggttgaggtc
      781 aacaaggctt gcaaggcgga ttaatgcgtc tggagcagat cgtgcatgca ggccgccgat
      841 cactttaatg ccggaaagac ggagatctgc aaaaaccgca agctcttcgg atcttctcat
      901 ctcatcaaat actacatagt ccgggcggag caaggtgaga acctcacctg ttgcagcgat
      961 actgccatcg agtgcagtgt actgcgtgat tttgtccggc accatgagat cgcgcggaga
     1021 ttcgatcgtt ttaacgatgt agttttccga ggagaggtag gttgcgatgt tctgttccag
     1081 ggttgatttc ccagatcccg gagttccgac gatgagcatt ccgccgttac cgatgttcaa
     1141 ccgttctttc agtgctaccg cgtagttgta ggagtcaaag gagacctcgc gggttggccg
     1201 cacaattgtt atttcgattc cgtccgagaa tggtggacga gttgtggtga tgcgcatcga
     1261 gccgatctgg gatacggtaa caccgggcat ctctacttcg acaaatccat ctgggtggcg
     1321 cttggcgcgt tcaagacatt cctgtgcaat tgcccgcagt tcgtattcgg ttgctggggt
     1381 gtcgcgaatc gtaacaagtt ttgattcacg aattgttccc ttgcgggcat agggtgtgac
     1441 ccgctctttg agatatacgg caaaggtgtt ttcatcgaag aatgcatcaa ttaacagcgg
     1501 ggcgaagttg tcggtgattt ctggtctgag gaagacaacg ttgattcctt tcgcacgcgc
     1561 aacctcggcc tgaacatagt cgcttgttag gaatgttgca ttgtgtgcga tggcaacctc
     1621 ccgtatcatg ctgtcaatct ctcctccgcc tgcgagtttg acctgttcaa gtttggggcg
     1681 aacacccaca tattctaggt tgatgaggcc ttcttccttc attttgcaga gtttctgcaa
     1741 ctctgagagg ccggaaaatc ctatttctct gccctggttt gcctgtgctt cgagttcggc
     1801 gaagacggct tcgggtataa ttatcgttgc gccacgatat tcaccctgtt ctataagggc
     1861 ggtgacgcgt ccgtcgatga cgacgcttgt atccggtacg agtatcataa aaaatctacc
     1921 attatgtatg ggttgtcaat atcttattag gtctactgta ttttgtgata gatctcttgc
     1981 gggtctttga gtctggggag tatgatcctt ccgtcaatgg tgcggaaaaa acaactaaag
     2041 tacccttcat ggcatgcaca acccgtttgt tccacaagat agagaaggca atcgtgatcg
     2101 caatctacac gaatctcgca gaccttttgc aggtgtcctg actcctctcc tttcttccaa
     2161 agtttctgcc ttgaacggct gtagtaatga gcaaatcctg tctctttggt aagcgtaacg
     2221 gcttccttat ttgcataggc aaacatgagg acagtttttg tggcaatatc ctgaacaatt
     2281 acaggaatga gaccgccgtc atatgtaaga ttgtcaagta catccgtcat ttctgctatg
     2341 atatgggttg gcggggaaaa taaggagttg gggcatgtaa tccctctgtg atgaattttc
     2401 atttctgatt aatggtcagt gctacgaaag ctctctctta ttcacacctt tttatatcct
     2461 tctgccacta atgataataa tatgagcaag aatggtctcc tgctggttgg acacggaagt
     2521 agacttcagt acaataaaga gctgatcacc accaccgcac agatgatggc cgagagaact
     2581 gataaatatc tgatcaaatc ctgtttcatg gaaaacagtg tcccaactgt tccggagggt
     2641 cttgatgcaa tgcgcaaaga agacttggat cttctggttg ttgtacctct gttccttgca
     2701 aagggaatcc atgtgcttcg tgatatcccc ggtctcctcg gtcttgaaag cggatccaat
     2761 cgtggtgtct ttacgcttga aaacgggagg gagattcctg ttgtgtatgc agagccgatt
     2821 ggcatcgatc cgttgcttgc ggaattgatg cttaaaaacg cagagaaagc agttactacc
     2881 tacctctgat atttggatgc acgtactggt tctcgataca attcacggcg gtgaggacat
     2941 tgcggaagca ttgcggcaac gcggagatac cgttgatgca gttgatgtgt atcgcggaac
     3001 aggcgttccg gaatcggttg ccgcgtcccg ccggtatgat ctggttactg ctcctgtgca
     3061 tttgatacct tcacacccgt tgcttgcaaa atctcctgta aaaacccacc atgagatggt
     3121 tcgggaactg gttgtccccc cccgcatttc tgttgaaatc accggtgccc gtggtaaaac
     3181 tacaactgca tttgcgctcg cacatttgat gactgatctt ggatgtggta tcctgcatac
     3241 aagcagcggt acttttcgga tgcctgatcg ggaattgctc tggagaaaaa gtatcacacc
     3301 tgcttcggtg attgcagcaa gtgcttccgc acaaaagttc ggtgcagagt ggctgattgc
     3361 agaggagtct gttggcgttg ctggctttgg aacccttggc atcctgacat ccggtgatga
     3421 c
//
LOCUS       DALUAJ010000050         3289 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_8887,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000050 DALUAJ010000000
VERSION     DALUAJ010000050.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8887"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            32..1051
                     /gene="asd"
                     /locus_tag="O0X37_01145"
     CDS             32..1051
                     /gene="asd"
                     /locus_tag="O0X37_01145"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HJK61567.1"
                     /translation="MIHVGVLGATGAVGQRFVQLLANHPWFTLSTLTASDRSAGKTYG
                     SVVNWRLDSPFPESVKDIVVSPTTVDAVKDCDIVFSALPADIATTLESEIADAGVGVF
                     SNASSHRMEEDVPLMIAEVNPEHAALIDVQKKNHNREGFIVTNPNCSTIMLATALAPL
                     RKFSFTNINVATMQAISGAGFDGVPAFGIYDNVIPYIGSEEEKMAREANKILGTFNGS
                     QITGAPFQVSASCNRVPVVDGHTLNVWIDIAATPDEVIEAFQTWQPPFTGLPTQPEKT
                     ILYLPEENRPQPRLDRNRGNGMTVSVGRVREGIRFVAMGHNTIRGAAGASVLNAELLH
                     TKKYF"
     gene            1164..1430
                     /gene="albA"
                     /locus_tag="O0X37_01150"
     CDS             1164..1430
                     /gene="albA"
                     /locus_tag="O0X37_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006325 - chromatin organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein Alba"
                     /protein_id="HJK61568.1"
                     /translation="MDNTVLIGNKPVMNYVLAVVTQFNAGNSEVIVKARGKAIVRAVD
                     TAEIVTRQFLPNVVKQSVTTSTDQVEMDNGPSNVSCIQIILAKS"
     gene            1442..1741
                     /locus_tag="O0X37_01155"
     CDS             1442..1741
                     /locus_tag="O0X37_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61569.1"
                     /translation="MSKKNTIAGVALVVACVLCVWYVSLPSTEEFSREGTVSLLGEKT
                     VISGDDGTVYIPSTNVLEDCEIGTRIWFTAVVNSSQSELLRGPYKPVDILNISCM"
     gene            1752..3005
                     /locus_tag="O0X37_01160"
     CDS             1752..3005
                     /locus_tag="O0X37_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HJK61570.1"
                     /translation="MYPIAVGILAVVFLLIVMRRIGHVRLPIWAVMTAGAVLALVTGS
                     ITPEAAVAAVNVDVIVFLFGMFVLGVAMERSGLLHTASVAAFAKASSRHQVMFWFIFL
                     MAGASAFLMNDTVAIIGTPVALYCSHRFGIPVTRMLVALAAAVTFGSVMTPIGNPQVL
                     MIAMSGGVPNAFCTFFVYLCVPALVSLYILYRMMIWHLPNTAVSSMYDRDSSSPEDPV
                     LERLTKVSLVLVFVVICLRIGLSFTGYELPLVLIALAAALPLLLLSRRRVELLRGVDW
                     STLLFFVSMFVLMAAVWNTGVIQSLLPESFTTSMPVLFGVSVLVPQLVSNVPYVALVL
                     PVLESAAAPVYLYLTLAAGSTISGTLTILGAASSVIIIQNAEKHGETMTFWEYFRVGI
                     PLTIAASLVFIGWIWICSLLFGATG"
     gene            complement(3023..>3289)
                     /locus_tag="O0X37_01165"
     CDS             complement(3023..>3289)
                     /locus_tag="O0X37_01165"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HJK61571.1"
                     /translation="QPQGPMAAQVRCPKCGTTAPAGTKFCPECGTSMVQPESGRCTNC
                     GAVLQPGAKFCPECGTKVGSPKCPACGSEVAAGAKFCPECGQKL"
BASE COUNT          742 a          793 c          866 g          888 t
ORIGIN      
        1 atgaatatcc ggcaaagcct atgaatatct catgattcat gttggcgttc ttggagcaac
       61 cggcgctgtt ggtcagcgtt ttgtccagct cctcgcaaac catccctggt ttaccctttc
      121 aacccttact gcctccgatc ggtccgcagg aaagacctac ggttctgttg tgaactggcg
      181 gcttgattct ccgttcccgg agtcggttaa agacatcgtt gtatccccaa ccactgttga
      241 tgcggtaaag gactgtgata ttgtgttttc cgcacttccc gcggacattg caacaacgct
      301 tgagtccgag attgcagacg caggtgtcgg tgtcttcagt aatgcgagct cccaccgcat
      361 ggaagaagac gttcccctaa tgattgccga ggttaatcca gagcatgcgg cactcatcga
      421 tgtgcagaag aaaaaccaca atcgtgaggg attcatcgtc acaaacccca actgttccac
      481 gattatgctt gcaacggcac ttgcccccct gcggaagttt tctttcacaa acatcaacgt
      541 tgcaaccatg caggcaatct ccggagcagg tttcgatggg gtcccagcct tcggaatcta
      601 cgacaacgtg attccataca tcggatctga ggaggaaaag atggcccgcg aagcgaacaa
      661 aattcttggt acgttcaatg ggtcacagat caccggagca ccgttccagg tctctgcatc
      721 ctgtaaccgt gtccctgttg tagatgggca cacgctgaat gtctggatcg atattgctgc
      781 aactccagat gaggtcattg aagcattcca gacctggcag ccgccgttta ccggactgcc
      841 cactcagccg gaaaaaacga ttctgtacct tccggaggaa aaccgtccac agccaagact
      901 tgaccggaac cgcggtaacg gtatgaccgt ttctgttggg cgtgtacgtg aaggtatcag
      961 attcgttgcg atgggacaca acaccatccg cggtgcagca ggtgcttcgg ttctgaacgc
     1021 cgaactgctg catacaaaga agtacttcta agacggctct tttggagact atctgggata
     1081 ttcccaagat tcccagatac tttcctgtat ccatgcagat acgatcaact gataacagat
     1141 tatcgctaaa tgatactacg acgatggata atacagtcct gattggaaac aaaccggtga
     1201 tgaactatgt actggctgtg gttacacagt tcaatgccgg gaactccgag gtgatcgtga
     1261 aggcacgcgg caaagccatc gttcgtgctg tagatacagc cgaaattgtg actcgccagt
     1321 ttcttccgaa tgtggtgaaa cagagtgtaa ccacctccac agatcaggtc gagatggaca
     1381 atggcccctc caatgtctca tgcattcaga tcatcctcgc aaaatcctga cctttttttc
     1441 tatgtcaaaa aagaatacga tagctggtgt ggcactggtt gttgcctgtg ttctctgcgt
     1501 gtggtatgtg agtctaccaa gtactgagga gttttcccgt gagggaacag tttctctgct
     1561 tggggaaaag accgttattt ccggggacga cgggacagtg tatattcctt cgaccaatgt
     1621 tttggaggat tgtgagattg gaacccggat ctggttcaca gcagttgtga attcctcaca
     1681 aagcgaacta ctgcgaggac cctacaaacc agtagacatc ctgaacattt cctgcatgta
     1741 ggcctctgtt tatgtatcct attgccgttg gtattcttgc agttgtattt ctgctgattg
     1801 tgatgcggcg aataggacat gtgcgtctgc caatatgggc agtgatgaca gcaggagcag
     1861 tacttgccct tgttaccggt tcgattacac cagaggctgc ggttgctgcg gtgaacgttg
     1921 atgtaatcgt ctttttgttc gggatgtttg tactgggtgt cgcaatggag aggtcaggtc
     1981 tgctgcatac agcatcggtt gcggcatttg caaaagcatc ctcccgacac caggtgatgt
     2041 tctggtttat ttttctgatg gcaggagctt ctgcatttct gatgaatgat accgttgcaa
     2101 tcattggaac accggttgca ctgtactgtt cccaccgctt tgggattcct gtaacacgga
     2161 tgctggtagc actcgccgcg gcggtaacat tcgggagtgt gatgacgcca atcgggaacc
     2221 ctcaggtact gatgattgca atgtcggggg gtgtgcccaa tgcattttgt acattctttg
     2281 tgtacctgtg tgtgccggct cttgtgagtt tgtacattct ttaccgtatg atgatatggc
     2341 atctgccaaa cacagcggta tcatcgatgt atgatcgtga ttccagcagt ccagaggatc
     2401 ctgtactgga gcgactgacc aaagtatcgc ttgttctggt gtttgtagtg atatgtttgc
     2461 gaattggact gtcctttacc ggatacgagt tgcctctggt actgatcgca cttgctgcgg
     2521 ctctgccgct cctgcttttg tccagacgga gagtggaact tctgcgtggg gtggactgga
     2581 gtacactgct gttctttgtc tcgatgtttg tactgatggc tgcagtgtgg aacaccggtg
     2641 tgattcagag cctgctgccg gagtcgttta caacgtcaat gccggttctg tttggtgtga
     2701 gtgttctggt acctcagttg gtgtcaaatg taccgtatgt ggcactggtt ctaccggttc
     2761 ttgagtcggc ggcagcacca gtctatctgt accttacact tgctgcggga agcacgatat
     2821 caggtacgct gacaatttta ggtgccgcaa gctcggtaat tatcatccaa aatgcggaaa
     2881 aacatgggga aacgatgaca ttctgggagt atttccgtgt tgggataccg ctgacaattg
     2941 cagcatcact ggtgttcatc gggtggatct ggatctgttc gctgctattt ggtgctactg
     3001 gttaaaagat ttaaaaaaat ggttagagtt tctgtccgca ttcaggacag aactttgcac
     3061 ctgctgcaac ctcactgcca caggcagggc atttcgggga tcccactttg gttccgcact
     3121 caggacagaa tttcgcaccg ggctgaagga ctgcaccgca gtttgtacat ctccccgatt
     3181 ctggctggac cattgaggtc ccgcattcag gacagaactt cgtacctgcc ggggcagtcg
     3241 taccgcattt agggcaacga acctgggcag ccatcgggcc ctgaggctg
//
LOCUS       DALUAJ010000051         2718 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_8951,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000051 DALUAJ010000000
VERSION     DALUAJ010000051.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8951"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="O0X37_01170"
     CDS             <1..704
                     /locus_tag="O0X37_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJK61572.1"
                     /translation="NQVTENVLNQILTEMDGIEPLNDVVILAASNRPDIIDPALLRSG
                     RFDRLVYIPEPTIADRRAILAVHMRNMPLENSSLDAAAEQLAGRSEEKVRALIGKYTG
                     KTLTLRQIKSATKKIEAEEGGLSVPQLRRMLTDLFAAQNVIFEDSVRREFIEKIAGST
                     DGFVGSDLESLCREAAMEALRRNGTTVTDADFAAAKLRVHPTMNDRVREYYEGIRHRF
                     KGGLPKQVQNLVEYQ"
     gene            779..1417
                     /gene="cofC"
                     /locus_tag="O0X37_01175"
     CDS             779..1417
                     /gene="cofC"
                     /locus_tag="O0X37_01175"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HJK61573.1"
                     /translation="MYFHALIPFKPVNPKTRLASVMTQEEREEFAEVMLGDVISAVRK
                     TGCTATLLCTSKYTCNEALVAIRKEGLNEAINWALPQFHCPALIIMSDLPMTTPGSLQ
                     RVLSTKADMAIVPGLGGGTNVIFVKHPEIFHVEYYGFSYRRHLQIAEELGLTVEIIDS
                     MRMSTDVDEPSDLVELMIHGHGNAREWLYEHGFALSVENGRVSVMRNGEKIA"
     gene            1410..2267
                     /locus_tag="O0X37_01180"
     CDS             1410..2267
                     /locus_tag="O0X37_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJK61574.1"
                     /translation="MPETAELITQAEELALAGKLDEALRLYDAAIAADHTNPLAWVGK
                     ASILKAQGRYTDAAGCFDAALAGIPAWSKEAAAEKDRMAAFTSMLSVLKAEALLYAEK
                     PDEALAAVDAADEVRDADAASLVVRGQAFVQKKEYEKAGDCFYRAEEWCNTHEDTMLT
                     QVWHCKVQLAKESGGVLAPPYAAEMYAAGVGFRPPKGTPEEILERGNNLRSAGLLYDA
                     LRYYDAAIAEGHRNKALILFLKGVVFEQLRRSDDALEAYSDALKADPAPEDEFRIRMR
                     WAGLRANRG"
     gene            2324..>2718
                     /locus_tag="O0X37_01185"
     CDS             2324..>2718
                     /locus_tag="O0X37_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJK61575.1"
                     /translation="MDATATESGLLQLTEIVLTAEVVNGNPALGVNDLPVALRPLFCP
                     DAPGVIARPVAIKEGQLKTHFPNLAARSLIDDGKNSYLVVNDLGQFAVTTFAPAMKWY
                     LRHAGSDAVMKNPALSLALETAGETSVSY"
BASE COUNT          738 a          690 c          704 g          586 t
ORIGIN      
        1 gaaaccaggt cacagagaat gtactgaatc agatcctcac ggagatggat ggaatagaac
       61 cactgaacga cgtcgtgata cttgcagcat ccaaccgtcc ggacatcatt gatccggcac
      121 tgcttcgttc cgggagattt gatcgcctag tgtatattcc tgagccaacg atcgcagaca
      181 gacgtgcaat ccttgcagta cacatgcgca atatgcctct tgaaaactcc tctcttgatg
      241 ctgctgcaga gcagcttgcc gggcgcagtg aggaaaaggt gcgggcatta attgggaaat
      301 acaccggaaa aacactgaca ctccggcaga tcaaatctgc taccaaaaag atcgaggctg
      361 aagagggagg actctccgta ccacagcttc gccgcatgct cacggatctg tttgctgcac
      421 agaatgtgat ctttgaagat tcagtgcgaa gagagttcat tgaaaagatt gccggatcaa
      481 ccgacgggtt tgtcggatcg gatctcgaga gcctctgccg cgaggcagca atggaagcac
      541 tccgccggaa tggtacaacg gtcaccgatg cagactttgc tgcagcaaaa ctgcgggtac
      601 atccaaccat gaatgatcgt gtccgtgagt attatgaagg aatccgccac cggttcaagg
      661 gaggactgcc caagcaggtt cagaatctgg tagaatacca gtaacaaata cccatttttg
      721 attatattaa caaccagtgg cgagaattaa aatgtaagca taccaaaata gaattactat
      781 gtacttccac gccttgattc ccttcaaacc ggtcaatccc aagacccgtc ttgcctctgt
      841 tatgacacag gaggaacgcg aagagtttgc agaggtaatg ctcggcgatg tcatctccgc
      901 ggtccgtaag accggctgta ccgcaacact cctctgtaca tccaaataca cgtgtaatga
      961 agcactggtt gcaattcgca aagagggttt aaacgaagca atcaactggg cacttcccca
     1021 gttccactgc ccggcactta tcatcatgtc cgatctgccg atgaccactc ccggaagtct
     1081 tcaacgggtt ctgtccacca aagcagacat ggcaattgta cctggactcg gtggtggaac
     1141 caacgttatt tttgtaaaac atccggagat cttccacgtg gagtactacg gattctccta
     1201 ccgccgccat cttcagattg cggaagaact tggtcttacg gttgaaatca tcgactccat
     1261 gcgaatgtca accgatgtgg acgagccctc ggatctagta gaactgatga ttcacgggca
     1321 cgggaacgcc cgtgaatggc tgtatgaaca cggctttgcc ttgtcagtgg aaaacggtcg
     1381 ggtaagtgtc atgcgcaacg gtgagaagat tgcctgagac tgcagaactc atcacgcagg
     1441 cggaagagct tgctcttgcg ggaaaactcg atgaggcact caggctgtat gatgcagcaa
     1501 ttgctgcaga tcatacaaat cctcttgcat gggtaggaaa agcctccatt ctcaaagcac
     1561 aaggaagata caccgatgct gccggatgct ttgacgcggc tcttgcagga attcctgcgt
     1621 ggagcaagga agctgcagcc gagaaggatc gcatggctgc gtttacatcc atgctctcgg
     1681 tgttaaaggc tgaggcactt ctgtacgcgg aaaaaccaga tgaggccctt gcagcagtag
     1741 atgcagcaga tgaagtcagg gacgcggatg cggcatcact cgtggtcaga ggccaggcat
     1801 tcgtacaaaa aaaagagtac gaaaaagcag gagactgttt ctatcgtgca gaagagtggt
     1861 gcaacacgca cgaagacaca atgctcacgc aggtctggca ctgcaaagtt cagcttgcaa
     1921 aagagagtgg cggcgttctt gcaccaccat atgcagcaga gatgtatgct gcaggagttg
     1981 ggttccgtcc acccaaagga acacccgaag agattctgga acggggaaac aatctcagaa
     2041 gtgccggact tctgtatgat gccctccgct attatgacgc agcaattgct gagggacacc
     2101 ggaacaaagc cctcatcctg tttctgaaag gggttgtctt cgaacaactc agacggagtg
     2161 atgatgcact tgaggcttac agcgatgcac tcaaagcaga tccagcaccc gaggatgagt
     2221 tccggatccg tatgaggtgg gcaggtcttc gggcgaatcg gggatagaaa catccctcac
     2281 aaaaccctta tgcccttttt tcacccatat atatggaata cctatggacg caacagctac
     2341 tgaatccgga ctgctgcagc ttacagaaat tgttctgaca gcagaggtgg taaatggaaa
     2401 cccggcgctc ggtgtaaatg atctccctgt agcactgcgt cccctcttct gcccggacgc
     2461 tcccggtgtc attgcacgac cggtcgcaat caaagaggga caactcaaaa cacacttccc
     2521 gaatcttgcc gcacgtagtc tcattgacga cggaaagaac tcgtatcttg tggtaaatga
     2581 tctcggacag ttcgcggtga ccaccttcgc acctgcaatg aaatggtatc tgcgccatgc
     2641 aggatcggac gcggtaatga aaaacccggc actatccctc gctctggaga ccgcgggcga
     2701 gacatcggtc tcatacaa
//
LOCUS       DALUAJ010000052         3894 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_8998,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000052 DALUAJ010000000
VERSION     DALUAJ010000052.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8998"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="O0X37_01190"
     CDS             <1..1153
                     /locus_tag="O0X37_01190"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HJK61576.1"
                     /translation="VAVLLLASLLFCGIAAAAEATSLTINVYDNSAAHDPVKDAKVVI
                     TKSGTSLSQTLYTTSDGIVEFPSVEYKSTYSVTVTKDGFDEQKFTIDINAMEKEYTVY
                     LQKSNLVQVKVLNTDKSTPVAGAAISVDGLAMGTTNSAGVLHVSMEKGVYHNIQVTAD
                     SYETYTSSQYIETDQTSLTITLSKSYFSPRILVYDADNDMKPVSLATVIIDGKTVGTT
                     DEYGRATLNDLTAGTYTLEVTKPNYNSYKNTVTFTEDSSDVIVELTYAVVPLTVLVVD
                     GNNPVAGATIYIDNLVTGLTDTTGKFTKDVEPGKTILITASKDGYATQSISWSVTADQ
                     NNTVTVPITQNFPVALVGGIIAVIIIIGVVVFLVKAKGSSDNRGRKGSI"
     gene            complement(1181..1252)
                     /locus_tag="O0X37_01195"
     tRNA            complement(1181..1252)
                     /locus_tag="O0X37_01195"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1217..1219),aa:Arg,seq:tcg)
     gene            1358..1789
                     /gene="dcd"
                     /locus_tag="O0X37_01200"
     CDS             1358..1789
                     /gene="dcd"
                     /locus_tag="O0X37_01200"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HJK61577.1"
                     /translation="MLLSSSAIRARINDGTLGITPFTEESLQPASYDLRAAEDTILKA
                     GELSLIATMEFVSLPADLAATLRGRSSFGRKGVLLGAGYIDPGFRGNLTLCMVNMGKE
                     DIAVEKGTRIVQMLIHPILGEVENVYNGNYQDSYGVVKSKI"
     gene            complement(1797..>3894)
                     /locus_tag="O0X37_01205"
     CDS             complement(1797..>3894)
                     /locus_tag="O0X37_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tubulin/FtsZ family protein"
                     /protein_id="HJK61578.1"
                     /translation="QHDERSGVRSVRSFVFDADQKTIAEIQSIPTEDRFVLTPLDPVK
                     EHNYHGTDFEVGNLASCFQRNGIEEIDAVFVCAGLGGRMAELVPTVVSQIADAFPDPV
                     FTILTLPGRNEGVRVSARASKTLADIRKISQATILFDNETWFKRTEDEDSIKNLVEAK
                     HKATIYEMFPVLNEMLARRVGLLLRAGEFNHKGVEAAEVVLDAGEVLNTLTGMDLVSI
                     GYATEKLPTTWTNNVLKRIRVEKYLLEENQARTSRIVELAKQAVYREVSVPCDLTSSE
                     KALILIAGPSDELSMKGFQTVRKWIDRSIKGLEMRAGDYPVKSTRYVGVIIVLAGLEN
                     VPRVKELDEIRAIYENEQSKEYEESDKTEKSPKAQIANDELLFEDEMVMPIGVANRSA
                     VPEVEDDIFELDDFTMQGTGDDVFEDEPRRVQYSADDADDVFEEVVSSPSQQRYMPPP
                     QYTPQYVQQTQTGTIFDEPLSEDRPVIPKGKPKAKEMPKITIAGPKKQPQMTGSTIMM
                     PKRQKRVDNVLAGMADVGGKDKPKDSDNVLSGKTRVGGNNRPKETITTDVLEGSVKVG
                     NQRPKETDGGVRMSGGQRPKETMVPGVLEGSVTVGTQRPKETDGHIRMAGSQRPKETD
                     GHIRMASSQRPKETDGVVSGGSAQRPKEIGEYPKVGDAQRPREPNKGIRVTSIPLPKN
                     PAGKAVTKKSEKDKWL"
BASE COUNT          897 a         1046 c          895 g         1056 t
ORIGIN      
        1 agttgctgtt cttctgctgg catccctgct attctgtgga atagctgcag ctgcagaagc
       61 tacaagcctt accattaacg tctatgataa cagtgcagcg cacgaccctg taaaagacgc
      121 aaaggttgta atcaccaaaa gcgggacctc tctgagccag acactctata ccacctccga
      181 tggtattgta gagtttccct ctgtcgagta caaatccacg tacagtgtaa ccgtcaccaa
      241 agacggattc gatgagcaaa aattcaccat tgacataaat gcgatggaaa aagaatacac
      301 ggtctatctc cagaagtcaa acctcgttca ggtaaaggtt ctcaacaccg acaaatcaac
      361 tccggttgcc ggtgctgcca tctccgttga cggacttgca atgggaacca ccaacagcgc
      421 aggagtactt catgtctcta tggaaaaagg cgtgtatcat aacattcagg taactgcgga
      481 ctcttacgag acctacacct cctcccagta catcgaaaca gatcaaacct cgcttacgat
      541 cacgctttcc aagtcgtact tctccccgag gatcctcgtc tatgatgctg acaatgatat
      601 gaagccggtc tcccttgcaa ccgtcattat tgatggtaaa accgtcggaa caaccgatga
      661 gtacggaaga gccaccctca atgatctcac tgccggaaca tatacactgg aagtcaccaa
      721 accaaactac aactcctaca agaacaccgt taccttcacc gaggactcct cagatgttat
      781 cgttgaactt acctatgcag tcgtgccgct gactgttctg gttgttgatg gtaacaatcc
      841 ggtcgccgga gctacgatct acattgacaa ccttgtaacc ggtctcaccg acacaacagg
      901 aaagttcact aaagatgtag agccaggaaa aaccattctg attactgcat ccaaggatgg
      961 ttatgccaca caaagtatct cctggtcggt aactgcggat caaaacaata ctgtgaccgt
     1021 gccgattacc cagaacttcc cagttgctct tgtcggcgga atcattgcgg tgatcattat
     1081 tatcggggtt gttgtgttcc ttgtaaaagc aaaaggatcg tcagataacc gcggcagaaa
     1141 aggatccatc taacccactt ttttcaaaac agacacagta cggacctgct ggggatcgaa
     1201 cccagatctc cgggttcgaa gcccggaagg atatccacta ccctacagat ccaatgtgat
     1261 attattcatc tatctttatc ccttttttac tctatcgtat ttttccggat ccccgcaaca
     1321 caaaatacaa ttaccactga aaacgaacaa aaaagatatg ttgctttctt cctctgcaat
     1381 ccgtgcacgc atcaacgacg gtacccttgg gattacacca tttactgaag aatcactcca
     1441 gccagcatcc tatgatcttc gggcagccga ggatacgatt ctgaaagccg gggagctctc
     1501 ccttattgca acaatggagt ttgtcagtct tcccgcagat cttgctgcaa ctctccgcgg
     1561 acgatcctca tttggcagga aaggagtgct gcttggtgct gggtatatcg atccgggctt
     1621 tcggggaaac ctaacgcttt gtatggttaa catgggtaag gaggacatcg cggttgagaa
     1681 aggtactcgc attgtacaga tgctgattca ccccatcctt ggagaagtgg aaaatgttta
     1741 caacgggaac tatcaggaca gttacggcgt ggtaaaatca aaaatataaa cggctcttag
     1801 agccacttgt ccttttcact tttctttgtg accgcttttc ctgcgggatt ttttggcaga
     1861 ggtatcgagg ttacccggat acctttgttt ggttcgcggg gtctttgtgc atcacccact
     1921 ttgggatact cgccaatttc tttgggacgc tgtgcagaac ccccgctcac gactccgtct
     1981 gtttcctttg gacgctggga actggccatt ctgatgtgac catccgtttc ttttggtctc
     2041 tggcttcctg ccattctgat atggccgtca gtttccttcg gacgctgtgt accaacagtc
     2101 accgaaccct caagaacgcc gggaaccatt gtctctttcg gtcgctgccc ccctgacata
     2161 cggacaccgc cgtccgtttc ttttggtctc tggtttccga ccttcaccga accttccagt
     2221 acgtctgtcg tgattgtctc tttaggccgg ttgttaccgc cgacacgcgt tttaccggag
     2281 aggacattgt ccgagtcttt tggtttgtct ttgccgccaa catccgccat tcccgcaagt
     2341 acattgtcca ccctcttctg ccgtttcggc atcatgattg tactgccggt catctgcggt
     2401 tgttttttcg gtccagcgat ggtgatcttc ggcatctctt ttgccttagg tttccctttt
     2461 gggatcaccg ggcgatcttc agagagaggt tcatcaaaga tggttccggt ctgtgtctgc
     2521 tgaacatact gtggcgtata ctggggaggg ggcatgtatc tctgctggga aggagaagag
     2581 accacctcct caaagacatc gtctgcgtca tcagcagagt attgcacccg ccgcggttca
     2641 tcttcaaaga cgtcatcccc agttccctgc atagtgaagt catccagctc aaagatatca
     2701 tcctctactt cgggaacagc agatctgttg gcaacaccga ttggcatcac catctcgtcc
     2761 tcgaacagaa gctcatcgtt cgcaatctga gcctttggac tcttttcggt tttgtcgctt
     2821 tcctcgtact ctttgctctg ttcattttcg taaattgctc gtatttcgtc caactccttc
     2881 acccgcggta cattctccag accggcaaga acaatgatca ctcctacata ccgggttgac
     2941 ttgacggggt aatccccagc ccgcatctcc agacccttga tgcttcggtc aatccatttt
     3001 cgcaccgtct gaaatccttt catcgagagt tcatcggacg gcccggctat caggatcagg
     3061 gctttttccg atgaggtgag atcacaggga acggatacct cccggtacac tgcctgtttt
     3121 gccagctcaa cgatacggga ggttcgtgcc tgattttctt ccagcagata tttctccacc
     3181 cggatgcgtt tcagtacgtt gtttgtccag gtggttggca gtttttctgt cgcatacccg
     3241 atggatacga gatccatccc tgtgagggtg ttgagtacct ctcctgcatc cagaaccact
     3301 tcagcggctt cgacgccttt gtgattgaac tcacctgcac ggagcaaaag accgacacgg
     3361 cgtgcaagca tctcgttgag gaccgggaac atttcgtaga tggttgcttt gtgttttgcc
     3421 tctaccagat ttttgatgct gtcttcatcc tcggttcgtt taaaccatgt ttcattgtcg
     3481 aagaggattg tagcctggga aatttttctg atgtcagcaa gtgttttgga cgcacgtgca
     3541 gagacccgta ccccttcgtt tcttccgggc agtgtaagga tggtaaagac cgggtccggg
     3601 aaggcatcgg caatctggga gaccaccgtt ggtaccagtt ctgccattct tccgccaaga
     3661 ccggcacaga caaagactgc atcgatctct tcgatgccgt tccgctggaa gcagcttgca
     3721 aggtttccca cttcgaaatc ggttccgtgg tagttgtgct ctttcaccgg atcgagcggc
     3781 gtgagaacga acctgtcttc tgttgggatg gattgtattt cagcgatggt tttctggtct
     3841 gcgtcaaaca caaaggatcg cacactccgg actccactcc gctcgtcgtg ctgg
//
LOCUS       DALUAJ010000053         2692 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_9137,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000053 DALUAJ010000000
VERSION     DALUAJ010000053.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9137"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            159..977
                     /locus_tag="O0X37_01210"
     CDS             159..977
                     /locus_tag="O0X37_01210"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HJK61579.1"
                     /translation="MKTILYYFTGTGNSLAVARAIAERLPETELVPIPMLLLKGETVR
                     VPKDTNIGIVYPLYAMGLPNIVIRFFGILDLSDAGYVFSVVTEGGRYGSPTKQIAALA
                     KQSGHDLNAAWWIQMPDNYIPLSAPPAKTEQKTIREDALRKVAVLVEAVQERRPRHID
                     LTATGKLLRTVMYKPFMKKIPAFGKKFVVSSNCNGCMVCVDVCPVNNIQVLPKGKKEW
                     LNHCEGCLACLQFCPVEAISCGGKTDERPRYHHPNITAADMKAQKGTAGIGHNE"
     gene            1051..1953
                     /locus_tag="O0X37_01215"
     CDS             1051..1953
                     /locus_tag="O0X37_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HJK61580.1"
                     /translation="MEFPRYDINKYDPKRMMLIPILIFLVAAAIVGFTFINTGMPVTP
                     GIDFAGGTAVTIHTTDTKEQIEAFYAGYPLTSIDEGVGNAGYYIKFGPMSNEEMMKLN
                     EETLAKYPDASIDQIGANFGATLQSQAMVALLFAFIGMAVVVFIAFRKLIPAATVVFA
                     GVADITITAGVMNIIGIELSLATTAALLMLIGYSVDSNILLTSKVLKRQGKLADKMAG
                     AFHTGFIMTTTTLAAIVAMLVVALIGQVPTLYNIAAVLVIGLVCDMIFTWAFNAGVLR
                     LYMEKEEGKKQPAQNKPKIKGAKS"
     gene            1950..>2692
                     /locus_tag="O0X37_01220"
     CDS             1950..>2692
                     /locus_tag="O0X37_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecD"
                     /protein_id="HJK61581.1"
                     /translation="MSEKKASGWINTVKDLRVLLVIVLVIIALAAIFVPIGDRGDAMT
                     NLQFGLALDGGSWIQLEFQAEVVTIGAGADVNDVAAKMGEYLDCTVTPIDQYHLEVQK
                     DATEEELRAAVAAAGASFIGYENGVGSTTAETVKRILESKVNSLGTNDVKVNTLTNTN
                     GVSQYVRVEMAGVDMQTAQDIVGKQGLFEIRIVTTGNETAHVLYGDSVSSVQNPTQNP
                     AGSDNWGVGFNLDNDGAVALQQACIQYGAT"
BASE COUNT          752 a          764 c          619 g          557 t
ORIGIN      
        1 ctttttttcc tgcatgagac acgcccaaac atcatcatac atcagaaaca cattcccctt
       61 ttatctaaca caatctataa ccacacgccc gacaaatata tgcacaggag ggacgccgga
      121 ctctatacca ataccaaaaa gtcaggccga tcaccgccat gaaaacaata ctctactact
      181 ttaccggaac aggcaacagc ctcgccgtag cccgggcaat cgccgagcgg ctcccggaaa
      241 ccgaactcgt ccccatcccc atgcttctcc tcaaagggga gaccgttcgc gtaccaaaag
      301 ataccaacat cggcatcgtt tatccgctgt atgcaatggg actgccgaat attgtgatca
      361 gattctttgg catccttgat ctctcggatg caggatacgt attctccgtc gtcaccgaag
      421 ggggaagata tggatcaccc accaaacaaa tagcagccct cgccaaacaa agcgggcatg
      481 acctcaacgc cgcatggtgg atccagatgc cggacaacta catccccctg tccgcaccgc
      541 ccgcaaaaac cgagcagaaa actatccgcg aagacgcact cagaaaagtt gcagtcctgg
      601 ttgaagcagt tcaggaacgg cgcccacggc acattgacct caccgcaacc ggaaaactcc
      661 tcagaacggt aatgtacaaa ccgttcatga aaaagatccc cgcattcggc aaaaaattcg
      721 tcgtcagctc aaactgtaac ggatgcatgg tttgtgtaga tgtctgcccc gtcaataaca
      781 tccaggtact ccccaaaggc aaaaaagagt ggctcaacca ctgcgaaggc tgccttgcct
      841 gcctgcagtt ctgccccgtt gaagccatct cctgcggagg taaaaccgat gaacgcccca
      901 gataccacca cccaaacatc accgcagccg acatgaaagc ccagaaaggc acagccggaa
      961 tcggtcacaa cgaataaccc tttttttcaa ttccaaactc ccggacaggg agggcttatt
     1021 atatcctttg aaaatcaata taaataaacc atggaatttc cccggtatga tatcaacaaa
     1081 tacgatccga aacggatgat gctcatcccg attctgatct tccttgttgc cgcggccatc
     1141 gtcgggttca cgttcatcaa caccggcatg cccgtcaccc ccggcattga ctttgccgga
     1201 ggtaccgcgg ttacaatcca caccaccgat accaaagaac agatagaggc attttatgca
     1261 gggtatcctc taacctccat tgacgaagga gtcggaaatg ccggatacta catcaagttc
     1321 ggcccgatgt ccaacgaaga gatgatgaaa ctcaatgaag aaacactcgc caaataccct
     1381 gatgcaagta tcgatcagat aggagcaaac tttggtgcaa cgcttcagtc acaggcaatg
     1441 gttgccctgc tcttcgcatt tatcggtatg gcggtcgtcg tattcattgc attccgcaaa
     1501 ctgattcccg cagcaaccgt cgtcttcgca ggagtcgctg acatcaccat caccgcagga
     1561 gtcatgaaca tcatcggaat cgaactctcg cttgcaacaa ccgcagcact cctcatgctc
     1621 atcggttact ctgttgacag caacattctc ctgaccagca aagtcctcaa acgtcaggga
     1681 aagctcgccg acaagatggc aggtgcattc cacaccggat tcatcatgac cacaacaacc
     1741 cttgccgcaa tcgttgccat gctggtggtt gccctcatcg gtcaggttcc aaccctttac
     1801 aacatcgcag ccgtcctagt catcggtctt gtctgcgata tgatcttcac ctgggcattc
     1861 aatgcgggtg ttctccggct ctacatggaa aaagaggaag gaaagaagca gcccgcacag
     1921 aataaaccaa agattaaggg ggcaaaatca tgagcgaaaa gaaagcatcc ggatggatta
     1981 acaccgtcaa ggatctgcgt gtccttctgg tcatcgttct ggttatcatt gcacttgctg
     2041 caatctttgt accgatcggc gatcgcggag atgctatgac caatcttcag tttggtctcg
     2101 ctctcgacgg aggatcctgg attcagcttg agttccaggc agaagttgtc accatcggcg
     2161 ccggagctga cgtgaacgat gttgcagcaa agatgggaga gtatctcgac tgcacagtaa
     2221 cgccaattga tcagtatcat ctggaagtcc agaaggatgc aaccgaagaa gaactccggg
     2281 ccgcggtcgc agcagccggt gcgagcttca tcggatacga aaacggagtg ggatctacaa
     2341 ccgccgagac agtcaaacgg attctggagt ccaaggtaaa cagtcttgga accaatgacg
     2401 tcaaggtcaa caccctgacc aacaccaacg gagtttcaca gtatgtccgt gttgagatgg
     2461 caggcgttga catgcagacc gctcaggaca tcgttggaaa acagggtctc tttgagattc
     2521 ggatcgtaac aaccggcaac gaaacagccc atgtgctgta cggtgactcg gtatcatcag
     2581 ttcagaatcc cacacagaac ccggcaggtt ccgacaactg gggtgttgga ttcaacctgg
     2641 acaacgacgg agcagtggca ctgcaacagg cctgtatcca gtatggagca ac
//
LOCUS       DALUAJ010000054         2729 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_9298,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000054 DALUAJ010000000
VERSION     DALUAJ010000054.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9298"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="O0X37_01225"
     CDS             <1..491
                     /locus_tag="O0X37_01225"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832776.1"
                     /note="catalyzes the reversible formation of
                     methenyl-H(4)methanopterin from
                     N(5)-formyl-H(4)methanopterin; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HJK61582.1"
                     /translation="IAEKCKVDVANVCALVAPTSSIVGSIQVAGRCVETAVYKLNELG
                     FDTRKILTAAGTAPIPPVRGPKLAMGVTNDATIYHGQINLTMNAPEIVDYLEKIPSCA
                     SKGYGKPFNEIFKEAGYDFYKIDTSLFSPAEVVINEVSTGKVYQVGKVDVDVTLKSFG
                     LA"
     gene            569..642
                     /locus_tag="O0X37_01230"
     tRNA            569..642
                     /locus_tag="O0X37_01230"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:603..605,aa:Val,seq:tac)
     gene            complement(664..1029)
                     /locus_tag="O0X37_01235"
     CDS             complement(664..1029)
                     /locus_tag="O0X37_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HJK61583.1"
                     /translation="MTSFQEIFLWTGSLYIPILLAVLGAAFWKHPQKTISGNIGYRTR
                     RSRQSQAAWDAAQKLSGKYLCILGIAQMIAVPVVLLLASPVCDPFSLSMICMTAEVLL
                     IAVPVIAVEHYLKIHFPKQ"
     gene            complement(1026..1592)
                     /locus_tag="O0X37_01240"
     CDS             complement(1026..1592)
                     /locus_tag="O0X37_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJK61584.1"
                     /translation="MSGEDGINNGEYWFNRGENERDCGNLRQAQLCFERAVELDAEVP
                     KYWAELGLTFSECGEHEIAIKCLRRATDLDPKCAESWCGRGVAHCRAGDIDAALFAFD
                     RAVRLNPEVSDYWLNLGLMFAELDEHTKAVTAFECGLEINLYDEELWKQKAKCLIALG
                     EDIRARHCYERAGEIEMERATRPDTGNV"
     gene            1672..2535
                     /locus_tag="O0X37_01245"
     CDS             1672..2535
                     /locus_tag="O0X37_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61585.1"
                     /translation="MARKKEEFDPLAGPIGGGGIRKKKSKEKTFDVLGGGFGGDHPVE
                     KESFDSYSDVSFGGSSDALMETGGSSSSNAKPKKKGLFASIFGGGRKKKQKKSPAKSQ
                     PLEQETEKPESYVPKHLLDHELEIHDGVMETGIVDRMVDPAYDRRPASQGRYEDPVSD
                     SLGGEVSEEDLFEGEETRQRPVQRLAEKKAAPAPVSADVTYGSSSVMEDSHVCYLCGA
                     EFPAALPPFGLAPGKSVPLCRTCSHAVKTLKTFRDPSDEREIKLEWYTLCPNLDSDRA
                     DVIIAEVRRDF"
BASE COUNT          664 a          698 c          708 g          659 t
ORIGIN      
        1 agattgcaga gaagtgtaag gttgacgttg ccaacgtctg cgcacttgtt gcaccgacct
       61 cctccatcgt cggttccatc caggttgcag gacgctgtgt ggaaactgcc gtgtacaagc
      121 tgaacgaact cggctttgac acccgcaaga tccttaccgc agcaggtacc gccccaattc
      181 cgccggtccg cggccccaag cttgccatgg gcgtcaccaa tgatgcaacg atctaccacg
      241 gacagatcaa cttaacgatg aacgctccgg agatcgttga ctaccttgag aagatcccaa
      301 gctgcgcatc caagggatac ggcaagccgt tcaacgagat cttcaaggaa gcaggctacg
      361 acttctacaa gatcgacacc agcctgttct ccccggcaga ggtcgtcatc aacgaggttt
      421 ccaccggtaa ggtctaccag gtcggtaaag tcgatgttga tgtgaccctg aagtccttcg
      481 gtcttgcata agaaaagatg gggatctctt ccccttctat ttccgcagaa ttaattagga
      541 tacagcgact atagaataga gcattagcgg actcgtggtc taggtggtta tgacgtcgcc
      601 cttacatggc ggaggtcagg ggttcgaatc ccttcgggtc cactcgtttt tgaaaaattg
      661 ttttcactgt ttggggaaat gtattttcag atagtgttca acagcaatga ccggaactgc
      721 tatcagcaga acctcggcgg tcatgcagat catggacagc gaaaacggat cacagaccgg
      781 acttgcgagg aggagaacga cagggaccgc aatcatctgg gcaattccaa gtatacagag
      841 atattttccc gaaagtttct gtgcagcatc ccatgccgct tgtgactgcc ttgatctgcg
      901 ggtacggtag ccgatatttc cggagatggt cttttgagga tgtttccaga atgctgcacc
      961 gaggactgcg agaaggatcg ggatatagag tgaccctgtc cagagaaaaa tctcctgaaa
     1021 tgaggtcata cgttccccgt atccggtcgt gttgcccgtt ccatctcaat ttctccggca
     1081 cgctcgtaac agtgtcgtgc ccggatatcc tccccaagcg caatgaggca ttttgccttc
     1141 tgtttccaga gctcttcgtc ataaagattg atctcaagtc cgcactcaaa tgcagttacg
     1201 gcttttgtat gctcgtcaag ctcggcaaac atcagaccca gattcagcca gtaatcagaa
     1261 acttctggat ttaaccggac cgccctgtca aatgcgaata gtgctgcatc aatgtctcct
     1321 gcccggcagt gagcaactcc tctgccgcac cagctctcgg cgcattttgg atcgagatcc
     1381 gttgcccttc tgagacattt gatggcaatt tcatgttcgc cgcactcgct gaaggtaagg
     1441 cccagttcag cccagtactt gggaacctct gcgtcaagtt caaccgcccg ttcaaaacac
     1501 agctgggcct gccggaggtt accgcagtca cgttcgttct ctccgcggtt aaaccagtat
     1561 tccccgttgt ttatcccgtc ttcaccactc atctatacag agatcactcc agaggtatta
     1621 ataggtgggc gttgcatatg aaagtagaaa tagacccggt tgggaagcac tatggcacgc
     1681 aaaaaagagg agttcgatcc gcttgctggc ccgattggag gcggcggaat acggaaaaag
     1741 aagtccaagg agaaaacgtt tgatgtactt ggcgggggat ttggcggaga tcatccggtt
     1801 gaaaaggagt cgtttgacag ctacagcgac gtatcgtttg gcggttcatc cgacgcactg
     1861 atggaaaccg gaggcagttc ttcatcgaat gccaaaccca aaaagaaggg gctgtttgct
     1921 tcaatatttg gcggcggcag gaaaaagaaa cagaaaaaat cacctgcgaa atcccagccc
     1981 cttgagcagg agacggagaa gccggagtct tacgtgccaa agcatctcct tgatcatgaa
     2041 cttgagattc atgacggtgt aatggagacc ggtattgttg accggatggt cgatcctgca
     2101 tatgatcgga gaccagcatc tcagggacga tatgaagatc ccgtctccga ttccttgggt
     2161 ggagaggtgt ctgaggagga tctctttgaa ggagaggaaa ccaggcaacg gccggtacag
     2221 agattagcgg agaaaaaggc ggcacctgcc ccagtctctg ctgatgttac ctacggctcc
     2281 tcatctgtaa tggaagactc ccatgtgtgt tacctgtgcg gtgctgaatt ccccgcagca
     2341 ctcccgccgt ttggacttgc ccccggaaaa tccgtgccgc tgtgccggac ctgctcacat
     2401 gcggtgaaaa cgctgaaaac cttccgcgac ccgtcggatg aacgtgagat taagctggag
     2461 tggtatacac tttgcccgaa tctcgactcg gatcgtgccg atgtgattat tgccgaagtg
     2521 agaagggact tctaatcttt ttactcatat ctgagttgct tatcatttcg gcatcttaat
     2581 agcacacctc tctctaatgg attagtatgg attctctgcg tctgaaccgt cagcagtatc
     2641 tggtggcggt taccgttgcg attgcatgtt tcctgcctcc gtttattgga gaggcgacaa
     2701 acattgcact tccgtccctg atatcagat
//
LOCUS       DALUAJ010000055         6433 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_9983,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000055 DALUAJ010000000
VERSION     DALUAJ010000055.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6433
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9983"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /gene="trxA"
                     /locus_tag="O0X37_01250"
     CDS             <1..444
                     /gene="trxA"
                     /locus_tag="O0X37_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HJK61586.1"
                     /translation="ILIRLFFPYSGMEDEELEQLRKKRFEQLAAKIPPPEGWPVIELT
                     EYNFNEVTLGRPKVVIDFWAEWCGPCRYFSPIFEEMSVEYPEVQFCKCDTDRNHGIAK
                     QLGITSIPRVLYVINGTAVRLHVGAMSAEKFREELNAVFRSNDTV"
     gene            431..2584
                     /locus_tag="O0X37_01255"
     CDS             431..2584
                     /locus_tag="O0X37_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJK61587.1"
                     /translation="MTLFESFHPSLQEVMLSGLGWNGLRPVEEETYRAVAGGADVVVL
                     APTAGGKTESAFIPVIDALLKTGSRGLGAIYLSPLKALINDQQDRVLFMCGRAGLTVA
                     SQHGDVAARDRWKFSPEADTPNLLLTTPESLEVLLGDPDSRSAFSSLRFVVIDEIHAF
                     METDRGVHLRCLLDRLERITKNTIVQRIGLSATVGNPEELLAWMSGPNRKKQLVQIPS
                     PATPKQFSFVVEAEFTAQIRAVADAVRGKKALVFVDSRSFAERLMTPLSDLVPGVYLH
                     HSSVSAEDRAEAEAAFDQSGGTCVICTSTMELGIDIGDLDLVVQYGPPRSVASFLQRL
                     GRTGRRGRPAAMTFILQSPCDLLISAATIEAAMRHEIEPLRPPAHAYHVMVQQLFLLL
                     RSRTCGISRQTIITALRSLSPFAQVPPKTIEDLLWYLEEQGYVVRDGELYSPGPHAER
                     ELGRSNWIALISVIHDSGGYLAVLPDGTVVGTLDPRFVGSDPGKTFSFTGKNWRLLFR
                     DDVHKRALVEPASASGNGVKRPFWSGNGMRGADASPTVCDAVCQILARGKTVLPLPEE
                     QRTMVEDLIRILPEDFVPGTVHVRAEPEVAGWSVVIATFLGVRANMVLARLLKNRLSD
                     GKYSLRYDQFAIRIFGWESPDAAERTGYLLREVSHTDTVVLAEEMPELPEGTWKFAAM
                     LPKKMLHEMAVDEYYRLPEVLEAIRTVRVRDVSHR"
     gene            complement(2575..3348)
                     /locus_tag="O0X37_01260"
     CDS             complement(2575..3348)
                     /locus_tag="O0X37_01260"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HJK61588.1"
                     /translation="MGERYWEVDAVRGISLIGMIIFHITSLMVIFHMIFTLDWYYGVC
                     RYIHLGTSIFVIISGVALVLRYGRMAGHSKREYHVAILKRGIEIFLIGIAIAVIASVA
                     IHFFVRDGNYMLFNFLQMMGLSMIICIPFLWLGKWNIIPAAILIWAGFVIKSIHTGPI
                     WLLPLGVLPDGMFYPRDYFPLLPWVGVMLLGVAIGSVLYPNGVRRFHIPDAGSLGKVF
                     SLVGRHTLAIYLLHLPIIAAILYVIMVVSNALGMPFGYL"
     gene            complement(3473..3697)
                     /locus_tag="O0X37_01265"
     CDS             complement(3473..3697)
                     /locus_tag="O0X37_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61589.1"
                     /translation="MTTSAAKKAEIVAYLEQIVAIQNEGDNDVVEVRIAKLGDGIHHS
                     KELNHHEKHTAGEILKIRDWNALKKYLENM"
     gene            complement(3781..4398)
                     /locus_tag="O0X37_01270"
     CDS             complement(3781..4398)
                     /locus_tag="O0X37_01270"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HJK61590.1"
                     /translation="MNGLLLKDWISLRAAIVLVVIVFVFCTALFVPWGVSSVAFLIGI
                     AMALLLPVNTMTLDTASRWERYVLALPLSRREILAARYQFAYLCLGVVALICGIAAVV
                     SVLFLDGYDLLGVSPLIWWTGCMGVALLILDLMLAVYFVYRMNIGILLFVVVCFVPTL
                     AVYVQDLMVPALLSPLAFVLLLAGLLGLWISWRVALQAFECWDAA"
     gene            complement(4412..5059)
                     /locus_tag="O0X37_01275"
     CDS             complement(4412..5059)
                     /locus_tag="O0X37_01275"
                     /inference="COORDINATES: protein motif:HMM:NF024741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HJK61591.1"
                     /translation="MNGLLYKDLLNLTPTLKYLVFMALIFCVVFIPLGNELPVYIILI
                     MFGALLPTTAISFDTAARWDKYAVSLPVTRREIVAARYQLMVGGICVAGVISLIIAVA
                     MMILMPGEGIFLPYFSPLMLMVLFVACGLLLGSIALPLTLKFGVEKMRYIIMVIALTP
                     VVVIFGMSFFADLSGITPTDPVLMPVIIGVLLAVTALFVYVSFWLSVRIYAKKEF"
     gene            complement(5056..5916)
                     /locus_tag="O0X37_01280"
     CDS             complement(5056..5916)
                     /locus_tag="O0X37_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK61592.1"
                     /translation="MEYAINVTGLCKSYQDFALDNVSFAVPKGSIMGFIGENGAGKST
                     TIKAMLNLIHRDAGTVEILGMDLDGHEKEIKERIGVVFDECCFHDNLTPADISKILGN
                     IYQNWDGKLYQKYLVQFGLDEKKKIKEFSRGMKMKLGIAAALAHRPDVLILDEATSGL
                     DPIVREEILDIFLDFIQDENHAILISSHITSDLDKIADYLTFIHHGRIILSSGRDDLM
                     DSMGIVKCGAEDLEKIEQDQIIRYRKNQFGCEVLIRNKRSFAVGHPEMVIDPVTTEAI
                     MLFYSRGEEL"
     gene            complement(5920..6312)
                     /locus_tag="O0X37_01285"
     CDS             complement(5920..6312)
                     /locus_tag="O0X37_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HJK61593.1"
                     /translation="MTQEVLVNIIISNASDKPIYEQITSQIKRMIISGELPEGTALPS
                     MRLLAKELRISVITTKRAYTDLEHDGFIETVPGKGSFVAGSNLEIIKEEQMRLCEEHL
                     QKAVDIATQSGITYEELRESLELLYNGE"
BASE COUNT         1769 a         1554 c         1627 g         1483 t
ORIGIN      
        1 atcctcatta ggttgttttt cccatattca ggtatggaag atgaagagtt ggaacagctg
       61 cgaaagaaac ggtttgaaca gctggcagca aagataccac caccggaagg gtggccggtc
      121 atagaactga ctgagtacaa cttcaatgag gtaacacttg gtcggccaaa ggtggtgatt
      181 gatttctggg ccgagtggtg cgggccatgc cggtatttca gtccgatatt tgaggagatg
      241 tcagttgagt atcccgaagt acagttctgt aaatgcgata ccgatcgtaa tcacgggatt
      301 gcaaaacaac ttggcattac cagtattccg cgggtactgt atgttataaa cggaactgcg
      361 gtgcggctgc atgtgggtgc aatgtctgcg gagaagtttc gcgaggagtt gaacgcagtg
      421 ttccgatcca atgacactgt ttgagtcctt tcacccaagt ctgcaggagg ttatgctttc
      481 gggtctggga tggaatgggc tgcggccggt tgaggaggag acataccggg cggttgccgg
      541 aggcgcagat gtggtggtac ttgcgccgac agctggggga aaaacagagt cggcattcat
      601 tccggtgatt gatgctctgc tgaagacagg ttccagagga cttggagcga tctatctgtc
      661 gccgttgaaa gccctgatta atgatcaaca ggacagggtt ctgtttatgt gcgggagggc
      721 aggactgact gtggcatccc agcacggcga tgtagcagca cgcgaccgat ggaaattttc
      781 tccagaggca gacaccccga atcttctgct cacaacaccc gaatcactgg aagtactgct
      841 tggagatccc gattcacgga gtgcattttc ctcactccgg tttgtcgtga tcgatgagat
      901 tcatgcattt atggaaacgg atcgtggcgt acatctccgg tgccttctgg atcgcctgga
      961 aaggatcaca aaaaatacta ttgtgcagag gataggactc tcggcaacgg ttggaaatcc
     1021 ggaagaactg cttgcatgga tgtccgggcc aaatcgaaag aaacaacttg ttcagattcc
     1081 gtcccctgca acaccgaaac agttctcttt tgtagtggag gcggagttta ctgcacagat
     1141 acgggcggta gcagatgcag tacggggaaa aaaggcactg gtgtttgtgg acagccggag
     1201 ttttgcagaa cggctgatga caccgctcag tgatctcgtc cccggagtgt atcttcatca
     1261 ctcatcggtg tcagcagagg atcgtgcaga agcagaggcg gcgtttgatc agagcggggg
     1321 gacctgtgta atatgcacaa gcacaatgga gctcggcatt gatatcggtg atctggatct
     1381 ggtggtgcag tatgggccgc cgcgatcggt tgcatcattt ctgcaacggc ttggacgaac
     1441 agggagacgg ggaagacctg cggcaatgac atttattctg caaagtccct gtgatctgtt
     1501 gatctctgca gcaacgattg aagcggcaat gcggcatgag atagaaccgc tcagacctcc
     1561 tgcacacgca tatcacgtga tggttcagca gttgtttctt ctgctgagaa gccggacctg
     1621 cggcatatca cgacaaacga taattacggc acttcgatcg ctttcgccat ttgcgcaggt
     1681 cccaccaaaa accatagagg atctgctttg gtatcttgaa gagcagggat atgtagtacg
     1741 cgacggagaa ctctactccc ccggacctca tgccgaacgg gagctgggaa gatcaaactg
     1801 gattgcgttg atctcggtga ttcatgattc aggaggatat cttgcagtgc tgccggacgg
     1861 aacagttgtg ggtacactgg atccacggtt tgtgggaagc gaccccggca agacattttc
     1921 attcaccggg aaaaactgga ggctcttgtt ccgtgacgat gtacacaaac gtgcgctggt
     1981 ggaacctgca tctgcctcag gaaacggagt gaaacgaccg ttctggagtg ggaatggtat
     2041 gcggggggcc gatgcctccc ccactgtctg tgatgcggtc tgtcagattc tcgctagggg
     2101 aaagacagtt ctgccgttgc cggaagagca gagaacaatg gttgaggatc tgatccggat
     2161 actgccggag gattttgtac cgggaactgt tcatgttcgt gccgagccag aggtcgccgg
     2221 atggtccgtg gtgatcgcaa catttctggg agttagggca aacatggttc ttgcacgttt
     2281 gctgaaaaac agactttctg atgggaaata cagtctgcga tatgatcagt ttgccatacg
     2341 gatctttgga tgggagtccc cggatgctgc ggaacggact gggtatctcc tgcgcgaagt
     2401 atcgcataca gatactgtag tgcttgccga agagatgccg gaactacctg aaggaacctg
     2461 gaagtttgca gcgatgctac cgaaaaagat gcttcatgag atggcagtgg atgagtatta
     2521 ccgtctgccg gaagtacttg aggccatcag gaccgtacgg gtacgggacg tatctcacag
     2581 ataaccaaac ggcataccaa gcgcattcga gacaaccata atgacgtaaa ggatagcagc
     2641 aatgatgggg aggtgcagga gatagatcgc gagcgtatgt ctgccgacaa gagagaagac
     2701 tttgccgagt gatcccgcat ccgggatatg gaaacgacgg acaccgttgg gatacaggac
     2761 cgagccgata gcgacaccaa ggagcatcac acccacccag ggaaggagcg ggaagtagtc
     2821 acgaggatag aacatgccgt ctggaagaac accgagcgga agcagccaga tcgggccggt
     2881 atggattgat ttgatcacaa atcccgccca tatgaggata gcagcaggga tgatgttcca
     2941 tttgccaagc cagagaaacg ggatacagat gatcatggag aggcccatca tctgcaggaa
     3001 attgaagagc atgtagttgc cgtcacgaac aaagaaatga attgcaacgg aggcaataac
     3061 agcaatggca atcccgataa ggaagatttc aatgccgcgc ttaagaattg ccacatggta
     3121 ctcacgtttt gaatgtcctg ccattctccc gtatcgaaga acaagggcaa caccggatat
     3181 gataacaaaa atacttgttc caaggtgaat gtagcggcag accccatagt accagtccag
     3241 ggtaaagatc atgtgaaaga ttaccatcag ggaagtgatg tgaaaaatga tcatcccgat
     3301 gagagagata ccccgaactg catcaacttc ccagtaccgc tcgcccattg cctcaacgtt
     3361 tgtgttgtat gataatggct tttgtgcttg tgggataggt gtaaaaatgc tgggattcac
     3421 aggcaggcat gctaaaaaag agatgagatg acggatttcg aaaaaaaaga gattacatat
     3481 tctcaagata ctttttcagc gcattccagt cgcgaatctt cagaatttca ccggctgtat
     3541 gtttttcgtg atggttcagc tcctttgagt ggtgaattcc gtcgccaagt tttgcgatac
     3601 gtacctctac cacatcatta tcgccttcat tctggattgc aacaatctgt tcaagatatg
     3661 cgacaatttc tgcctttttt gcagcgctgg ttgtcataaa aagtaatcat ccctcatagg
     3721 atatacacac tttgatagcg gcagagaaag aacaccggat cccaacagca ctgaaagaga
     3781 tcatgccgca tcccaacatt caaacgcctg caatgcaaca cgccatgaga tccataatcc
     3841 caggagaccg gccaggagaa gtacaaacgc taagggtgaa agcagagcag ggaccatcag
     3901 atcctgtaca tagaccgcga gggttggaac aaaacacaca acaacgaaca gcagaattcc
     3961 gatgttcata cgatacacaa aatacaccgc aagcatcaga tccagtatca gcaacgcaac
     4021 acccatacac ccagtccacc agatcagtgg cgacaccccc agcagatcat atccatccaa
     4081 aaaaagtacg gatacaactg ctgcaatacc gcagatcaag gccactactc ccagacacaa
     4141 atatgcgaac tgatatcggg cggccagaat ttcccggcgg gaaagtggca gtgcaagcac
     4201 ataccgctcc caccggctgg ctgtatccag ggtcattgta ttgaccggga ggagaagagc
     4261 catcgcgatc ccgataagaa atgcgacaga actgacaccc cacggtacaa acagtgcagt
     4321 acagaaaacg aacacgataa ccacaagcac aatagcagcg cgcagggata tccagtcctt
     4381 cagcagcagt ccattcatat gttaccctac tttagaactc cttttttgcg taaattcgaa
     4441 ctgacaacca gaaggataca tagacaaaga gtgctgttac agcaaggaga acgccaataa
     4501 tcacaggcat cagtaccgga tcagttggag ttatgcctga caggtccgca aagaatgaca
     4561 taccgaatat caccacaacc ggtgtcagcg caatcaccat gatgatatag cgcatcttct
     4621 ccaccccgaa tttcagcgtc agcgggagag caatgcttcc cagcagcaga ccgcaggcaa
     4681 cgaacagcac catcagcatc agcggactga agtagggaag aaaaatacct tcacccggca
     4741 tcagtatcat catcgcaact gcaatgatca gtgagatgac accggcaaca caaatgcctc
     4801 caaccatcag ctgataacgt gctgccacaa tctcccgacg ggtcaccggc agacttaccg
     4861 cgtacttatc ccaccgggct gctgtatcaa aactgatcgc agttgtcggc agcagtgcgc
     4921 caaacataat caggataata tacaccggca gctcatttcc cagtggaata aacaccacac
     4981 agaagatcag tgccatgaat accaggtact tcaacgtcgg tgtcagattc agcagatcct
     5041 tgtacagcag accgttcaca gctcctcacc ccgcgagtag aacagcataa tcgcctcggt
     5101 cgttaccggg tcaatcacca tctccggatg cccgacagca aacgatctct tgttgcggat
     5161 caaaacctca caaccaaact ggtttttgcg gtagcggatg atctgatcct gctcgatttt
     5221 ttccagatcc tcggcaccgc acttcacaat acccatactg tccatcagat cgtcacgacc
     5281 agatgaaaga atgatgcgac cgtgatggat gaacgtcaga tagtctgcga tcttatcgag
     5341 atcactggtg atatgggatg agatcagaat cgcatggttt tcatcctgaa tgaaatcaag
     5401 gaagatatca aggatctcct cgcggacaat tggatcaaga ccacttgttg cctcatccag
     5461 aattaataca tccggcctgt gagcaagagc cgccgcaatg ccgagtttca tcttcatacc
     5521 gcgggaaaat tctttaattt tctttttttc atcaagaccg aactggacaa gatacttttg
     5581 atatagtttc ccgtcccaat tctgatagat atttcctagg attttggaaa tgtcagctgg
     5641 agtaagattg tcgtggaaac agcactcatc aaacaccacg ccaattctct ccttaatctc
     5701 cttttcatga ccgtcaagat ccatgccaag aatctcaacg gtacccgcat cacggtgaat
     5761 gagattcagc attgccttga tcgtcgttga ttttcccgca ccgttctccc cgatgaatcc
     5821 catgatagat ccttttggta cggcaaagga aacattgtcc agtgcaaaat cctgatacga
     5881 tttgcaaaga cctgttacgt ttattgcata ctccatgtct tactctccat tgtaaagcag
     5941 ctcaagagac tcccgcagct cctcatacgt gataccgctt tgggttgcga tgtccactgc
     6001 cttttgcaga tgctcctcac acagtcgcat ctgttcctct ttgataatct caagatttga
     6061 tcccgccaca aagctgcctt tcccgggaac tgtttcaatg aatccgtcat gttccagatc
     6121 agtataggca cgttttgtgg tgatgacact gatgcgcagt tcctttgcaa gaagacgcat
     6181 acttggcagg gcagttccct ccggaagttc gccgctgatg atcatgcgtt ttatttgcga
     6241 ggtaatctgc tcgtaaatgg gtttatcact ggcattgctg atgatgatgt tcacgaggac
     6301 ctcctgcgtc atattgtata tattgtatat acacattaat acttttgcat ggaagaaaga
     6361 ctcagcatct ggagattttc caaaaaccta tcttcaggca tatttatcag atgacacaac
     6421 caatgtatta ccc
//
LOCUS       DALUAJ010000056         2463 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_10135,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000056 DALUAJ010000000
VERSION     DALUAJ010000056.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2463)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2463)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2463
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10135"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="O0X37_01290"
     CDS             <1..923
                     /locus_tag="O0X37_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HJK61594.1"
                     /translation="TEAVTMIARGLSWKPGDVIVTTVLEHHSNLLPWRALEKFGVTLR
                     VVGLRPDLTLDMDAFAAAMDASVRLVAVTQASNVTGTIVPVAEIAELCRRYGALLAVD
                     AAQSAPHMQIDVRKLGVDFLSFSGHKMCGPTGTGVLWMKEPILEPLFLGGGMVEHVTD
                     DGFTPAEGFHRYEAGTPNIAGGIGLGEAAEFLQEIGMPQIEARERELSCRLIEKLSAI
                     PGVRVYAPSDPVMRIGVVSFTIDGISPHEAAQYLDDEGGILVRSGMHCAEPLMRYLGC
                     PEGTLRASLAFYNTESEIDTLAATVREMVS"
     gene            920..1633
                     /locus_tag="O0X37_01295"
     CDS             920..1633
                     /locus_tag="O0X37_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HJK61595.1"
                     /translation="MKTVLTLLVDCAGVFEGSRTAAANMPVKKFIGCLPPSLPVRILR
                     YSDTAMWHIGPEPVLAGDLEWTDIPSGGYLSSLAHAANLAGQSLQIAPDVQNIVLLIS
                     DGALSDPEEIVLAVLAKRFDPSVIRAAVPLTTDADSAVLRMFAGDNVYDPSVLLDPRP
                     FLSSFGESPAPSVSHPATGQIVLSCTIALDGGDSFSLSVAGMDLDQVKEEMKTSLAGF
                     GTRDDQIRQKIAKYISRVL"
     gene            1786..>2463
                     /locus_tag="O0X37_01300"
     CDS             1786..>2463
                     /locus_tag="O0X37_01300"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HJK61596.1"
                     /translation="MKYVVVTGGVMSGLGKGITAASIGRILINRGYHVTSVKIDPYLN
                     IDAGLMNPAQHGEVFVLKDGGEADLDLGNYERFLDIELTRDHNLTTGKIYSSVIQKER
                     HGDYLGATVQIIPHITDEIKDRIKHAAESWTDKDGNHAEICIVEVGGTVGDIESMPFL
                     EAVRQMHWEYGNGDFVLVHVTLLPADTMGDLKTKPTQHSVKALRELGLEADLIVGRST
                     LPVSLSTK"
BASE COUNT          520 a          664 c          665 g          614 t
ORIGIN      
        1 ccaccgaagc ggtcactatg atcgcccgcg gactttcgtg gaaacccggg gatgtcattg
       61 ttaccacagt acttgagcat cactcaaacc tccttccctg gcgtgcgctg gagaagttcg
      121 gtgttacgct gcgggtggtt ggtctccggc cggatcttac gcttgatatg gatgcgtttg
      181 cagcagcaat ggatgcctcc gtccggctgg ttgccgtaac acaggcatcg aatgtgaccg
      241 gtaccattgt cccggttgcg gagattgcag agctgtgccg gaggtatggt gcacttcttg
      301 ctgttgatgc cgcacagtct gctccccaca tgcagattga cgttcgaaag cttggtgtgg
      361 attttctctc cttctccggt cacaaaatgt gcggcccgac cggaaccggt gtactctgga
      421 tgaaggaacc tatcctggaa ccgctgtttc ttggaggtgg gatggttgag catgtcactg
      481 atgatggttt cacaccggcg gagggtttcc accggtacga ggcgggaaca ccaaacatcg
      541 cgggcggcat tggtcttgga gaggctgccg aattcttgca ggagattggt atgccgcaga
      601 tcgaggcgcg ggaacgggaa ctctcctgca ggctgatcga aaaactctct gcaattcccg
      661 gcgtccgggt ctatgcaccg tcagatcctg tcatgcgcat aggtgttgtc tcctttacca
      721 ttgacggcat atccccgcat gaagccgcac agtatttgga tgacgaaggc ggcattctgg
      781 tgcggtccgg aatgcactgt gctgaaccgc tgatgcggta tctcggctgt ccggaaggaa
      841 ccctccgtgc aagtcttgct ttctacaaca ctgaaagtga gattgacacc cttgcggcaa
      901 ctgttcggga gatggtttca tgaaaaccgt cctgaccctt cttgtggact gtgccggtgt
      961 gtttgagggc agcagaaccg ccgctgcaaa catgccggtg aaaaaattca tcggatgcct
     1021 ccctccctcc ctgccggtcc gtatcctccg ttactcggac actgccatgt ggcacatcgg
     1081 tcctgagccg gttctagctg gggatctgga gtggacggac atcccgtcag gaggatatct
     1141 ctcttccctt gcccatgcgg caaatcttgc gggacagagt cttcagatag cgcctgatgt
     1201 acaaaatatt gttcttctca tctctgacgg cgccctctcc gatcctgagg agattgtgct
     1261 ggctgtgctt gcaaaacgct ttgatccatc cgtcattcgc gccgccgttc ctcttaccac
     1321 ggatgcggac agtgccgtcc tccgtatgtt tgcgggagac aacgtctatg atccgtcagt
     1381 tcttcttgac ccgcgtccct tcctctcctc atttggagag tccccggctc cgtcggtttc
     1441 acacccggct actgggcaga ttgttctttc ctgtactatt gcgcttgacg gcggagactc
     1501 gttttccctt tctgttgccg ggatggatct tgatcaggtc aaggaggaga tgaagacatc
     1561 tcttgcaggc tttggtaccc gtgacgatca gatcagacag aaaatcgcga agtacatctc
     1621 gcgggtactg taacctttcc cctttttgtt ttcacttttt tgggtgcctg tatcttgtgt
     1681 aaatcctgta ttactcctgc ccaaagaaca ctcaacccct cagtttcaat acctctgccc
     1741 tccaatataa cagcattatt taatttgaaa cagcggggac acaagatgaa gtatgtagtt
     1801 gtaaccggcg gtgtgatgag cggccttgga aaaggcatta ccgctgcgtc cattggacga
     1861 attctcatca acagaggcta ccacgtaacc tccgtcaaga tcgacccgta cttaaacatt
     1921 gacgcgggtc tcatgaaccc ggcccagcac ggagaagtct ttgtgttaaa ggatggtggt
     1981 gaggctgacc ttgatctggg aaactatgag cggttccttg acatcgagct tacccgggat
     2041 cacaatctga ccaccggaaa gatctactcc tctgttattc agaaagagcg ccacggcgat
     2101 tatcttggag cgaccgttca gattattccg cacatcaccg acgagatcaa agacagaatc
     2161 aaacatgccg cagaaagctg gaccgacaag gacggcaacc acgcggagat ctgtattgtc
     2221 gaggtaggag gaacggtcgg tgatattgaa agtatgccgt tccttgaggc ggtccgtcag
     2281 atgcactggg agtacggcaa cggcgatttt gttcttgttc acgtaaccct tcttccggca
     2341 gatacgatgg gtgacctcaa gacgaaaccg acgcagcact ccgtgaaagc actgcgtgaa
     2401 ctcggtctcg aagctgatct gatcgtcggc agaagcaccc tgccggtctc gctttccacc
     2461 aag
//
LOCUS       DALUAJ010000057         3328 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_10160,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000057 DALUAJ010000000
VERSION     DALUAJ010000057.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10160"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="O0X37_01305"
     CDS             complement(<1..439)
                     /locus_tag="O0X37_01305"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HJK61597.1"
                     /translation="MYSEKLNIPTALTFDDVLIEPAESWTEPSDVDVKSKFSKNIPLS
                     IPLVSSAMDTVTESAMAIALARAGGIGVLHRNCTAEQEVAFVERVKNAEKVIERDVRY
                     VTPDTTIGFVANLMEMHGIGGVPVVGPKGKLVGIVSRRDVRGVI"
     gene            complement(445..1134)
                     /locus_tag="O0X37_01310"
     CDS             complement(445..1134)
                     /locus_tag="O0X37_01310"
                     /EC_number="4.2.3.153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HJK61598.1"
                     /translation="MELLVSPSSIEEAKFCLDADIIDVKRPAEGSLGANFPWVIREIK
                     KLAGTKPVSAAIGDYGPTPGNAALAAYGAACAGADFIKIGLMFNDKDAARDVIEGVVR
                     AVKEPFPEKTVVIAAYSDYARLGTIAPLAMSPIAAECGADFAMIDTGMKDGKSTFEFM
                     NKEALQEFTDQNRSLGLKTALAGSLKFEDIPVLKEINPEIIGVRGMVCGGDRSTMVKA
                     ELVAKAIQMVR"
     gene            1242..1925
                     /locus_tag="O0X37_01315"
     CDS             1242..1925
                     /locus_tag="O0X37_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HJK61599.1"
                     /translation="MDVVLAVDLKGGYVVHGCSGNRETYAPLTWGLSPSAEPSAYLTV
                     MQPKYLYVADLDRIAMCGDHTEQILRLAERVTELWVDRGCSIPEEYLREKNIHNIVGT
                     ETIDAPLDEFDGGFLSIDIIDGCVIGNAFGPGTLPKEALHAADETNFDGCILLNISSV
                     GTTTGIDPSYAEQLRSSTEKKLYYGGGVRSVDDLHILADSGFDGAIIATAVHKGNIPL
                     EVVQEGSFC"
     gene            1919..2512
                     /gene="tmk"
                     /locus_tag="O0X37_01320"
     CDS             1919..2512
                     /gene="tmk"
                     /locus_tag="O0X37_01320"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HJK61600.1"
                     /translation="MLITLEGIDGSGKSTLYNGLKDRLADLDPLFTREPGSPYLGDAV
                     RRAIAANDDPMVEAALFVADHAVHLADVVRPALAEGRIVISDRYSDSRFAYQEVSLAN
                     VHPNPHRWLQEVHAGWSIRPDMTILLVLPVTVAMSRLAGRTEQEHFEQQEFLENVQKN
                     YLARAAEDPERFVLVDADRDAETILDFAEKSIRAALQ"
     gene            2603..>3328
                     /locus_tag="O0X37_01325"
     CDS             2603..>3328
                     /locus_tag="O0X37_01325"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HJK61601.1"
                     /translation="MIKLQTPTEKIAGVPLNNHMLLAAGVLGTTAASLSRMLRLGAGG
                     VVTKSIGPEPKDGHHGPSLIPVSGGILNAMGLPNPSQEFTEEIAPLLAAKEPVVVSIF
                     GGTPAEFAKVAEWFPEALAFELNLSCPHAEGYGAAIGANPRVVRECTESVKTFGKPVW
                     VKLTPNVADIGVIGRAAEEGGADAIVAINTVKAMRISTGMRRPVLGNKFGGLSGTAVF
                     PIAVRAVYDLYEACTIPIIGCGGV"
BASE COUNT          851 a          873 c          828 g          776 t
ORIGIN      
        1 gaatgacacc gcgcacatca cggcgagata caatcccaac cagcttccct ttcggaccta
       61 ccacaggaac accgccgatg ccatgcatct ccatcaggtt tgccacaaat ccaatcgttg
      121 tatccggtgt tacataccgg acatcacgct caataacctt ctcagcgttt ttgacacgct
      181 caacaaaggc aacctcctgt tctgcggtac agtttcgatg caaaactccg ataccgccag
      241 cgcgtgccag agcaatcgcc attgccgact cggtaaccgt atccatggca gaactcacga
      301 gcggaattga caacggaata ttcttcgaaa atttcgactt cacatctacg tcgcttggct
      361 cggtccagga ctccgccggt tcaatcagca catcgtcgaa cgttaatgcc gttggaatat
      421 tgagtttctc gctgtacatg ttacttacct aaccatctga attgcttttg ctacaagctc
      481 tgctttgacc atcgttgaac ggtcaccgcc acaaaccata ccacggacac cgatgatttc
      541 cgggttgatc tcctttaaga caggaatatc ctcaaacttg agagaaccgg caagggccgt
      601 tttcaaacca agcgagcggt tctgatcggt aaactcctgc agagcctctt tgttcataaa
      661 ctcaaacgta ctcttaccgt ccttcatgcc agtatcaatc attgcaaaat cagcaccgca
      721 ctcagcggca atcggagaca ttgccagtgg ggcaatcgtt ccaagccggg cgtagtcaga
      781 gtatgctgca atgactacag ttttctccgg gaacggctcc ttgacggcac gaaccacacc
      841 ttcgatgacg tcacgggctg catccttgtc gttgaacatc agaccgatct taataaaatc
      901 tgcaccggcg catgccgcac cgtatgcagc aagagcagca ttgcccggag taggaccata
      961 gtccccgata gcagcagaaa ctggtttggt tcctgcaagt tttttaattt cgcggattac
     1021 ccacggaaaa ttcgcaccaa gcgacccttc ggcaggacgc tttacatcaa taatgtcagc
     1081 atcgaggcag aactttgcct cttctataga gcttgggctg actaagagtt ccatggatat
     1141 tatatggtct ttacccttaa taagagcatt cacttttagt ccctgaagtt gaaaatccgt
     1201 ggggacgttt tattctcctg cgcgtcatat agaatagcaa aatggatgtt gtacttgcag
     1261 tagatctcaa gggtggatat gtggtacacg gatgttccgg taaccgtgaa acctatgcac
     1321 cgctgacctg gggtctgtcc ccctcagcag agccgtctgc atatctcaca gtgatgcagc
     1381 cgaagtacct gtatgtagct gaccttgatc gaatcgcgat gtgcggggac catacagaac
     1441 agattctccg gttagcagag cgggttacag aactctgggt tgatcgagga tgcagtatcc
     1501 ctgaagagta tctgcgagag aaaaacatcc acaatattgt aggaacagag acgatcgatg
     1561 ccccgttgga tgagtttgac ggcgggtttc tgagcattga cataatagac ggatgcgtga
     1621 tcggtaacgc gtttggacca ggaaccctac ccaaagaggc actgcatgca gcagatgaaa
     1681 cgaactttga cggctgtatt ctcttgaata tctcatcggt cggtaccaca acaggtatcg
     1741 atccctcata cgccgaacag ctacggagca gtaccgaaaa gaaactctac tatggagggg
     1801 gagtaagatc agtggatgat ctgcacattc ttgccgacag cgggtttgac ggcgcaatca
     1861 ttgcaacagc tgtacacaaa ggaaatatcc cactcgaagt tgtgcaggag ggatcatttt
     1921 gctgattaca ctggaaggca tcgacgggtc cggtaagtct acactctaca acgggctgaa
     1981 agacagactt gcagatctgg atcctctctt cacccgcgaa ccaggaagcc cgtaccttgg
     2041 tgatgcggta cgacgtgcaa ttgctgcaaa cgatgacccg atggtagagg ccgcgctgtt
     2101 tgtcgcagac catgcagtac atctcgcgga tgtggtccgt cctgcattgg ccgaaggccg
     2161 catcgttatt tccgatcggt actctgactc acgatttgcc tatcaggaag tatcacttgc
     2221 aaacgttcac cccaaccctc acagatggct gcaggaagta catgcgggat ggtcgattcg
     2281 accggatatg acaatccttc ttgtcctgcc ggttacagtg gcaatgtcac gcctggcagg
     2341 aaggacagaa caggaacatt ttgagcagca ggagttcttg gaaaacgtac agaagaacta
     2401 tcttgcccgt gccgcagagg atccggaacg gtttgttttg gtggacgcgg atcgggatgc
     2461 agagacaatt cttgattttg cggaaaaaag tattcgtgca gccttgcagt aactcctgag
     2521 atggtgttgc tccggttgcc tcctcctatt ttatcgcctc gttagaatta atgcagaacg
     2581 aacaacatac aataacacag atatgatcaa gctgcagaca ccgacagaaa agatagccgg
     2641 ggtccctctc aacaaccata tgcttctcgc tgcaggagta ctcgggacca ccgccgcatc
     2701 gctgtcacga atgcttcgtc tcggggcagg cggagtagtc acgaaatcaa ttggaccgga
     2761 accaaaggac ggacatcacg gaccgtcact cattcccgtc tctggtggaa ttctcaatgc
     2821 aatgggactg ccaaatccct cacaggaatt taccgaggaa attgcccctc ttcttgctgc
     2881 taaggaaccg gttgtcgtga gtatcttcgg agggacaccg gcagagtttg ctaaagttgc
     2941 cgaatggttc cccgaagctc ttgcgtttga actcaatctc tcttgtccgc atgcagaagg
     3001 gtacggtgct gcaatcggtg caaacccgcg ggttgtgcgg gaatgtacgg aatccgtcaa
     3061 aacgttcggc aaaccggtct gggtcaaact taccccaaac gttgctgaca ttggcgtgat
     3121 cggcagagct gccgaagaag gcggcgcaga cgcgatagtc gccatcaaca cagtaaaagc
     3181 aatgcggatc tcaaccggca tgcgccgccc agttctcgga aacaaattcg gaggattgtc
     3241 cggaactgcc gtgttcccaa ttgcggtacg cgcagtgtat gatctctacg aagcatgtac
     3301 cattcccatc attggctgcg gcggagtc
//
LOCUS       DALUAJ010000058         3729 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_10336,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000058 DALUAJ010000000
VERSION     DALUAJ010000058.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10336"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="O0X37_01330"
     CDS             complement(<1..1059)
                     /locus_tag="O0X37_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61602.1"
                     /translation="MAVLVVLLAAALFAGSVTATDMTNDKDFANITANVSTLTAAAGF
                     ASEDIKIFNKTALTDTTKLDVYVDWGDDNIEKVILAQTDGNEYNATVSHPYAKAGTYY
                     VKLYNASSYTAGKTLYKNLVTITVVNAREVGNGDDVFVYEHIIYQNRDATKLVKYSDG
                     ASPSVVNQISGTNGIFYLTEDVVGGQYGTYAVENATGQIGTISVWYPEISLQAELTTG
                     DNGETSGDSIDGKTITKDTRVSFLINSPKVQPALAGEEEENQASAKIVFTTPAGGQTT
                     YFGESDANYLGNIPLSGTQTIVGGMTIGTNAEPGTWTAQAEYLTVPFNKYAKKSNTVT
                     FTVQSTALAVSAAKDSVIR"
     gene            complement(1501..2343)
                     /locus_tag="O0X37_01335"
     CDS             complement(1501..2343)
                     /locus_tag="O0X37_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-dependent methylenetetrahydromethanopterin
                     dehydrogenase"
                     /protein_id="HJK61603.1"
                     /translation="MVVKVGVAKLGNIASGVMAELLLDERADREDMMTFMATSGTKLQ
                     KEDVDRVVSNLKAWQPDFAIVVSPNGVLEGPTGAREDLAAAGIPTIVITDDVTTKKEQ
                     FAALKESKFGYIIVKADAMIGARREFLDPIEMADYNGNLVKVLAVTGAFRKLQLELDK
                     VIDQVKAGKKGDELVLPKVVLNSDNSTKGEFNNPYALAKARAAYEIAQSVAGVNVKGC
                     FMTKEWTDYVPIVASAHEMMRVAAVLCDEARELEKAGDSVIRKPHKKTGEIVSKVALI
                     SKPE"
     gene            2712..3116
                     /locus_tag="O0X37_01340"
     CDS             2712..3116
                     /locus_tag="O0X37_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfx family DNA-binding protein"
                     /protein_id="HJK61604.1"
                     /translation="MKDTLLTDRQKEVIRYRKQGMTQQQIADRLGTSKANICTIEKSA
                     NENIRRAKETLEFLYTLDAADLCVLVAGTDLMEAPHLIYQAAAPLNIKIRYDSISLIN
                     RISSYMPEKIKGRHVREDIRVYLNTDGDLYFG"
BASE COUNT         1021 a          907 c          809 g          992 t
ORIGIN      
        1 gcggataacg gagtcctttg ctgcagacac cgcaagagca gtgctctgta ccgtgaaggt
       61 caccgtgttg gacttctttg cgtacttgtt gaacggaacg gtcagatact cggcctgtgc
      121 agtccaggtt cccggttctg cattggtacc aatggtcata ccacctacaa tggtctgggt
      181 gccggaaagc gggatgttgc caaggtaatt ggcgtcactc tctccgaagt aggtggtctg
      241 accaccagcc ggagtggtaa agacaatctt tgcacttgcc tgattctcct cctcctcacc
      301 cgcgagtgcc ggctggacct tcggagagtt gatcaggaag gagacccgag tgtctttggt
      361 gatggtcttt ccgtcgatcg agtcaccgga ggtttcgccg ttgtcaccgg tcgtcagctc
      421 tgcctgcagg ctgatttcag gataccagac ggagatcgta ccaatctggc ccgttgcgtt
      481 ttcgacagcg taggttccat actggccgcc gactacatct tctgtgagat agaagatacc
      541 gttagttccg gaaatctggt tgacaacact cgggcttgca ccatcagagt actttacgag
      601 ttttgttgca tccctattct ggtagatgat gtgctcatag acgaagacat catcaccgtt
      661 tccaacttct cttgcattaa ctactgtaat tgttacaaga ttcttgtaca gggttttacc
      721 agcagtgtaa gaggatgcgt tgtacagctt aacataatag gtacctgcct tagcgtaggg
      781 gtgggatacg gttgcattat actcattacc atcggtctgg gcaaggatta ctttctcaat
      841 attgtcgtct ccccagtcga catatacgtc gagcttcgtg gtgtcagtaa gtgctgtctt
      901 attgaagatc ttaatgtctt cagacgcaaa gcctgctgcg gcagtgagag tactgacatt
      961 tgcagtaata ttggcaaaat ccttgtcatt cgtcatatct gtagcggtta cagatcctgc
     1021 aaagagagct gcagcgagca gaacgacaag aactgccatt attttcgtgt ttttcaagtg
     1081 taattcctcc taaatgagga ttcaatcaca acaggcaaac ataccggaaa atatggcaaa
     1141 tatccaaaaa caacaaacat aaaaacatcg aagagaatcc agaaaaagat actgtcataa
     1201 tatgatcaca caaaaatcta cctcaaaaac acaccaaatc cttctaaata ttgtattcag
     1261 aacatttcat cgatcaacga aatacactga tctaagtagc aacaactttc aatgcagtgc
     1321 atatgatgtg ctacataaga attgctgtta ggttgaatcc tacatctcgt ttatcattga
     1381 atcctactat ccaaacagaa gtacaactcg ttatcaaaca tctcgatgat gatctctgca
     1441 caatcggctt tcaccctaca cgcactgcag caaaaaagaa aaaaatattt ttgaatatac
     1501 ttactcaggc ttgctgataa gagcgacctt ggagacgatc tcaccagtct tcttgtgcgg
     1561 cttgcggata acagagtcgc ctgccttctc aagctcacgt gcctcatcgc acagaactgc
     1621 tgcaacacgc atcatttcgt gtgcagatgc aacgattggg acgtagtcgg tccactcttt
     1681 ggtcatgaaa cagcccttga cgttgacgcc tgcaacggac tgtgcaatct cgtatgctgc
     1741 acgtgccttt gcaagtgcgt acgggttgtt gaactcgccc ttggtggagt tgtcagagtt
     1801 aaggacaacc ttcgggagaa caagctcgtc gcccttcttg cctgccttta cctggtcaat
     1861 gactttgtca agctcaagct ggagcttgcg gaatgcaccg gttactgcaa gaaccttgac
     1921 aaggttgccg ttgtagtcag ccatctcaat tggatcaagg aactcacggc gtgcaccgat
     1981 catggcgtct gccttcacga tgatgtatcc gaacttggac tccttgagtg ctgcaaactg
     2041 ttccttcttg gtcgtcacat catcggtgat aacgatcgtc gggatacctg ctgctgcaag
     2101 gtcctcacgt gcaccggttg gtccctcaag aacaccgttc ggggaaacaa caattgcaaa
     2161 gtccggctgc catgctttca ggttggacac aacacggtca acatcctcct tctgcagttt
     2221 cgttccggag gttgccatga aggtcatcat gtcttcacgg tctgcacgct catctaaaag
     2281 aagctctgcc ataacaccgg atgcgatgtt accgagtttt gcaactccta ccttaactac
     2341 catatttatt acacctctca ttttcaatga gaacatgaaa ttattgacga gaacaaatat
     2401 aaggtttgtg aattgataat gcatcttcac aaaatacggt gaaatatttg gtattaaaga
     2461 aaaacttccc caaatcttgg catcttatct agtaccacat gaagaaacac ttccatctct
     2521 tacgcacaag aacccattca tcatcagtcg ctgctattct ctctttagga gttatcaaaa
     2581 tacctgtaaa tttgtgcgcg taatctccga aaacaattca ttggcgagat tctgattaca
     2641 cagtcaggta ctggaaaagt acagtacttt aaccaaatat gtttatagtt aacagactta
     2701 tattaataac catgaaagat actctcctta ccgatcgcca gaaggaagtt atcagatatc
     2761 gcaagcaagg aatgacccag cagcagatcg ccgatcgtct cgggacatca aaagcaaaca
     2821 tctgtacaat tgaaaaatct gcgaacgaaa atattcggcg tgcaaaagag actctcgaat
     2881 ttctctatac ccttgatgca gcagacctct gtgtccttgt tgcaggcact gatcttatgg
     2941 aagccccgca tctcatctac caggcagcag cccctctgaa cataaaaatc cgatatgaca
     3001 gtatctcctt aatcaaccgc atcagctcct acatgcctga aaagatcaaa ggccggcatg
     3061 tcagagaaga catccgggta tatctcaaca ccgacggcga tctctacttt ggataacctt
     3121 tgcgtactct cgctcatctt ttttcctcca ttacctttat tatctcactc ttccaataat
     3181 agtgaagcct tcataggctg atatcagcat agctgctgtg tatcgtggat atccagttgg
     3241 tttgaaaaca cgcagattct gtcgatgttt tcattatcgt gtaacccttt tgtgggaaat
     3301 cccgcaaagg attaagacct caataggtct gatgaagcgc gcagggcgaa cgtcagcagg
     3361 acgagcccat tctttgtgaa aaccaaagag cgcgtgaact gtcaggttct cttacgtgag
     3421 acttggaaag ccgcaactgt gcggcgcgca tgtaacatgc gtcattcggg cataaaaacc
     3481 acaagaacag acaagacgtt gtcgttcatg cggatatcct aatggatcaa ttgctacccg
     3541 tctttccgtc ttctaagcat ctgtgcatct gcacagagag cggagaccga cactagaatg
     3601 tacgtcccat ggtcgaaggc gatttgcaat atcgcaggct gatggtgaaa gaacggttgt
     3661 ttaataattg acaaccacag cagacgtctc tccttcccaa aggaacgaag agaccacagt
     3721 attaaggac
//
LOCUS       DALUAJ010000059         2588 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_10724,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000059 DALUAJ010000000
VERSION     DALUAJ010000059.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10724"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            21..1136
                     /gene="nifS"
                     /locus_tag="O0X37_01345"
     CDS             21..1136
                     /gene="nifS"
                     /locus_tag="O0X37_01345"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HJK61605.1"
                     /translation="MVEAMLPYFSEKFGNPSSVYGIGQENKAAVDEARAKVAAAINAE
                     PNEIYFTAGGTESDNWVLKGVAFANIRKGKHIITTAVEHHAILHAAEWLQSQGFEVTY
                     LPVDQYGMVSPADVEKAIRPDTILISVMYANNEVGTIQPIAEIGAIARAHGIYFHTDA
                     VQAVGHVPIDVKAEHIDMLSLSGHKFYGPKGIGVLYIRRGVRIQNLLHGGAQESRHRA
                     GTENVAGIVGLGAAIERSVAAMPEESARLTAMRDHMIRELLKIPASHLNGHPTQRLPN
                     NVNITFEYIEGEGILLLLNMFGICASTGSACNSASLEPSHVLTAMGVPHEIAHGSVRL
                     TLGERNTEEDVSYVLEKLPEVVGKLRSMSPLTPEELR"
     gene            1137..1571
                     /gene="nifU"
                     /locus_tag="O0X37_01350"
     CDS             1137..1571
                     /gene="nifU"
                     /locus_tag="O0X37_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HJK61606.1"
                     /translation="MYSEKVMDHFTNPRNVGVIEDANGVGEEGNPSCGDIMKMYLKIE
                     NDVIVDVKFQTFGCGAAIASSSMATEMIKGKTIEEAWQLSNKAVAEALDGLPAIKMHC
                     SVLAEETIHKAINDYLIKQGREPWGEPASCSSCGHDCDTCGE"
     gene            1670..>2588
                     /locus_tag="O0X37_01355"
     CDS             1670..>2588
                     /locus_tag="O0X37_01355"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HJK61607.1"
                     /translation="MLSPFEKINQFITKYPFVIGCILIVMILIGIYGATGVSMNTTIQ
                     TPDKTDHASYVYQDYRDNFQTNSIVLMVQASDVRDVDVMKCVTAFEEILRQQNGVESV
                     ESIYDLILSYSGGVVPENQAVLDELFSKIPESVLAKSMPNNQLLLVKIDPTIGMTDEN
                     QQILLDTLANLLPLANAPPGVSLTFTGTAALHKDMGASLGSDVGRLLGSAFLLMITAL
                     VLLFHHARYTLLSIVSVVTGLLMTFGIMGLFQIPLSMATMGALPILLGIGIDYAIQFH
                     SRLDDEIREHPLAEAIRITITKTGSAVFFA"
BASE COUNT          587 a          688 c          723 g          590 t
ORIGIN      
        1 caacgtatgc ggcccctgag gtggtggagg cgatgcttcc gtacttttcc gagaagttcg
       61 gcaacccgtc gtcggtgtac ggtatcggcc aggagaacaa ggctgcggtt gacgaagcgc
      121 gtgcaaaggt tgcagccgcc atcaacgcag agccgaatga gatctacttc accgcaggcg
      181 gcaccgagtc ggacaactgg gtcctgaagg gcgttgcctt tgccaacatc cgcaaaggaa
      241 aacacatcat caccaccgcc gttgagcacc acgccattct tcatgccgcc gagtggcttc
      301 agtcgcaggg atttgaggtg acgtatctgc ctgtcgatca gtacggcatg gtctcgcctg
      361 cggatgtgga aaaggcaatc cggcctgata cgattctcat atccgtcatg tatgcaaaca
      421 acgaggtcgg aaccatccag ccgatcgcag agatcggagc aatcgcacgt gcacacggca
      481 tctacttcca taccgacgcg gtgcaggcgg tcggtcatgt cccgatcgac gtcaaagcag
      541 aacacatcga catgctctct ctgtccggtc acaagttcta cggcccgaaa ggcattggcg
      601 ttctctacat ccgccgcggg gtacggattc agaatctgct gcacggcggt gcgcaggaga
      661 gcagacaccg ggccggaacc gagaatgttg cgggaatcgt cggcctcggt gctgcaatcg
      721 aacgatccgt tgcggcaatg cctgaggagt cagcgcgtct taccgcaatg cgggatcaca
      781 tgatccgcga acttttgaag attccggcaa gtcacctgaa cggacatccg acacagcgtc
      841 tgccgaacaa cgtgaacatt acgtttgagt acattgaagg agagggaatt cttctgctcc
      901 tcaacatgtt cggcatctgt gcctccaccg gcagcgcctg caactccgcg tcccttgaac
      961 cgtctcatgt actgacagca atgggcgttc cgcatgagat tgcccacggc tcggtccgtc
     1021 ttacgctggg agagcggaac acggaggagg atgtatcgta tgttcttgaa aaactgccgg
     1081 aggttgttgg aaaactccgg tccatgtcac ccctgactcc tgaggagctg agataaatgt
     1141 acagcgaaaa agtgatggat cacttcacca acccgcggaa cgtgggcgtt attgaggatg
     1201 caaacggtgt cggcgaggag ggaaacccct cctgcggcga catcatgaag atgtatctga
     1261 aaatcgagaa cgatgtcatt gtggatgtga agtttcagac gttcggctgc ggtgctgcca
     1321 ttgcgtccag cagcatggcg accgagatga tcaaaggcaa gacgattgaa gaggcctggc
     1381 agctctccaa caaagcggtc gccgaggcgc ttgacggtct gccggcgatc aagatgcact
     1441 gctcggtgct tgcggaggag acgattcaca aggcaatcaa tgattacctg ataaaacagg
     1501 gacgcgaacc ctggggagag cctgcatcat gctcctcctg cggtcacgac tgcgacacct
     1561 gcggcgagta agccgtactc atctgttttt ttcgcaatat tgacgctatc tttaccttta
     1621 agtgcgttgc cggcaagatg gtatcctata caagaccggg agccgtatca tgctgtcacc
     1681 gtttgaaaag atcaaccagt ttattacgaa gtatccgttt gtaatcggct gtatcctcat
     1741 cgtcatgatc ctcatcggca tctatggtgc gaccggcgtt tccatgaaca ccaccattca
     1801 gacgccggac aaaaccgatc acgcctccta tgtttatcag gactaccgcg acaacttcca
     1861 gacaaactcc attgtgctga tggtgcaggc cagcgacgtc cgcgatgtgg acgtgatgaa
     1921 gtgcgtcacc gcatttgagg agattctccg gcagcagaac ggtgttgaga gtgtggagag
     1981 tatttatgat ctgattcttt cgtacagcgg cggcgttgtt ccggagaatc aggcggtgct
     2041 ggatgagctg ttctccaaaa ttccggagag tgttcttgca aagagcatgc ccaacaacca
     2101 gcttcttctg gtaaaaatcg atccgaccat cggcatgacg gatgaaaatc agcagatcct
     2161 tttggatacg cttgcaaatc tgctgccgct ggcaaatgcc ccgccgggag tttccctgac
     2221 ctttacgggg actgcggcgc tgcacaagga tatgggggca agtcttggca gtgatgtcgg
     2281 aagacttctc ggctctgcgt ttcttctgat gatcaccgct cttgttctgt tgttccatca
     2341 cgcccgctac acacttctgt ccattgtttc tgtggtgacc ggtcttctga tgacgtttgg
     2401 tattatgggt ctgttccaga taccgctgtc gatggcgacg atgggagccc tgccgattct
     2461 tctcggtatc ggtatcgatt atgcaattca gttccattcg cggcttgacg atgagatccg
     2521 ggaacatccg cttgccgagg cgatacggat tacgatcacc aagacaggtt ctgcggtgtt
     2581 ttttgcaa
//
LOCUS       DALUAJ010000060         3848 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_10802,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000060 DALUAJ010000000
VERSION     DALUAJ010000060.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10802"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="O0X37_01360"
     CDS             complement(<1..333)
                     /locus_tag="O0X37_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HJK61608.1"
                     /translation="MDANTVIIIAGIVIALAFNFSNGRNDASNTVATVIATRALAPRT
                     AILIASICCLAGPFIYSTAVAKTIGKGIVDPGIFTPVLLFIGLVGAVIWVNACSHYGI
                     PVSSSHALV"
     gene            complement(339..968)
                     /locus_tag="O0X37_01365"
     CDS             complement(339..968)
                     /locus_tag="O0X37_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HJK61609.1"
                     /translation="MGLKSWVVPQDKFFFELFEEQVEIVCEAADLLVNIFADYTNIQE
                     KYRQMKDLEHRGDAMAHRAYDELNRTFITPIEPEEISRLVTALDDVIDFIDSGANMLM
                     IYEIEKPDAFMVEFASVIRKAAEEIRTGVAGLRTLNDPETIKGCCIEINRLENQGDDI
                     LSNALCSLFKTKDPVEIIKFKDIYEHFEIATDKCEDVADVFAAILIRHT"
     gene            1097..1702
                     /locus_tag="O0X37_01370"
     CDS             1097..1702
                     /locus_tag="O0X37_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HJK61610.1"
                     /translation="MQYFSYGTTETDALKEADPILGAAIDRLGHPNRPLMPDIFTALV
                     SSVIAQQISNKAAETVQGRVTDRCGGEVTPDRIAELTEEEIQSLGMSRRKAGYIRGIA
                     LAFVSGQINPQTLRKAGEDQIVRELTALPGIGVWTAEMLMLHSLQRPDICSWHDHGIR
                     RGMMRLYGLDTLTKAEFDEHRRRYSPYGSVASIYLWALAHE"
     gene            1705..2223
                     /locus_tag="O0X37_01375"
     CDS             1705..2223
                     /locus_tag="O0X37_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJK61611.1"
                     /translation="MSGTCSAVLIILPPYQIFLRPMRRKAAQLHDSDAREILQTGIWG
                     TLATADSGGQPYAVPMNYAVMKDTVYLHSAQEGHKLENVRENPLVSFSVVEKAEVLAN
                     TFDMAYRSVILFGTARFVEEKTEKQNALQMLMEKYSPDGHDAASEYINRNIERTAVIA
                     IEIQHITAKAAN"
     gene            2303..2791
                     /locus_tag="O0X37_01380"
     CDS             2303..2791
                     /locus_tag="O0X37_01380"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJK61612.1"
                     /translation="MDSLYLRYATPEDIPQILGFIRELAEYEHMENEVVATEELLHEW
                     LFVKQKAEVLIGETEGKAVGFALFFHNFSTFLGRAGIYLEDLYVKPEFRGRGFGRAFL
                     ARLAATAVERGCGRLEWWCLDWNRPSIEFYLSLGAVPMSDWTVYRICGNTLSELAKTD
                     RQ"
     gene            2861..>3848
                     /locus_tag="O0X37_01385"
     CDS             2861..>3848
                     /locus_tag="O0X37_01385"
                     /EC_number="4.1.2.43"
                     /EC_number="4.2.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,6,7,8-tetrahydromethanopterin
                     hydro-lyase/3-hexulose-6-phosphate synthase"
                     /protein_id="HJK61613.1"
                     /translation="MFLIGEALVGDGAELAHIDLMIGDKMGPVGTAFANGLTQLSAGH
                     TPLLGVIRPNLLPKPAVLIVPKVTLKHTEQVTQIFGPAQAAVSKAVADALEDGVFTGM
                     DVDDVVIVASVFISPEAKDFNKLYRFNYGATRLALSRALDKFPDTATVLKEKDRAAHA
                     VMGFKVQRLWNPPYLQVAMDLVDMKQVERVLREVPQNDHVIFEAGTPLIKQFGLHVIG
                     EIRKIRPNCFIVADLKTLDTGNLEARMVSNAGGDAAVVSGLAPIETIAAFIKEAKKCG
                     IYAIIDMLNVAEPAKLIENLAKMGGAALLPQYVEMHRAIDKEASGEYSWGDIK"
BASE COUNT         1009 a          938 c         1004 g          897 t
ORIGIN      
        1 aacaagagca tgtgaggagg aaaccgggat tccgtaatgc gaacaggcat ttacccagat
       61 taccgcaccg acaagaccga taaatagcag taccggtgta aaaatacccg gatcaacgat
      121 accttttcca atggtttttg caacggctgt tgagtagata aagggtcctg caagacagca
      181 gatggacgca atcagaattg cagtacgggg tgcgagtgca cgtgttgcaa tgacggttgc
      241 cactgtgttg gatgcgtcgt ttcttccgtt cgaaaagttg aacgcaagcg cgatcacaat
      301 gcctgcgatg ataataaccg tgtttgcgtc cattgaactc acgtgtggcg aatcagaatt
      361 gcggcaaaga catcggccac atcttcacat ttgtcggttg caatctcaaa atgctcgtaa
      421 atatctttga acttgatgat ctcgacagga tcttttgttt tgaacagact gcacagggca
      481 ttcgagagga tgtcatcccc ctggttttcc agacggttaa tctcaataca gcatcctttg
      541 atggtctcgg gatcgtttaa ggttcgaagc ccagcaacac cggtgcggat ctcctctgct
      601 gcttttcgga taaccgaagc gaactcaacc atgaaggcat cgggcttttc gatctcgtag
      661 atcataagca tattggcgcc cgaatcgatg aagtcaatca catcatcaag tgcagtcacc
      721 agccgggaga tctcttccgg ctcgatgggc gtgataaatg tccggttcag ttcgtcataa
      781 gcacggtgtg ccatggcatc gccgcggtgc tcaagatcct tcatctgccg gtatttttcc
      841 tggatatttg tatagtcggc aaagatgttc actaagagat ctgctgcttc acagacgatc
      901 tccacctgct cttcgaacag ttcaaaaaag aatttgtcct gtggtaccac ccagcttttc
      961 aaccccatcc tctcataacc tctttacaga tacatggtaa ggtatgttac atgaatcttt
     1021 gcctttgaag aaggtatttt ccaaagccaa atactactta tgagatctgc gtccatatct
     1081 gacggagtac aaaacaatgc agtacttctc ttacggcaca acggaaacag atgcactgaa
     1141 agaagcagat cccattcttg gagctgcaat tgaccgtctt ggtcacccca accgaccgct
     1201 gatgccggat atctttacag ctcttgtaag cagtgtgatt gcccagcaga tctcaaacaa
     1261 ggcagcagag actgtgcagg ggagggtcac tgaccggtgc ggcggagagg taacaccgga
     1321 tcgaattgcg gaacttaccg aagaggagat tcagtcgctt gggatgtcga ggagaaaggc
     1381 aggatatatc cggggtatcg ctcttgcttt tgtttccggg cagatcaatc cccaaacact
     1441 ccggaaagca ggtgaggatc agatcgtcag ggagctgaca gcgctaccgg ggattggtgt
     1501 ctggacagct gagatgctga tgctccactc gttacaacgg ccggacatct gcagttggca
     1561 cgatcatgga atacggcggg ggatgatgcg gctgtacgga ctggatacac tgacgaaagc
     1621 agagtttgac gaacaccgcc gtcgttactc accatatgga agcgttgcgt caatctacct
     1681 ctgggcactc gcacatgaat aaccttgtct ggaacctgct cagcggtatt aattatcctc
     1741 ccgccctatc agatattcct gcggcctatg aggaggaaag cagcacagct gcatgacagc
     1801 gacgcacggg aaatacttca aacagggatc tggggcacac ttgcaacggc agacagcgga
     1861 ggacaaccct atgcggttcc gatgaattat gcggtgatga aagatacggt ttacctccac
     1921 tctgcacaag aaggacacaa actggaaaat gtacgagaaa accctctggt gtccttttca
     1981 gttgtcgaaa aagcagaggt gcttgcaaat acatttgaca tggcataccg gtcggtaata
     2041 ctctttggaa ctgccaggtt tgtagaggaa aaaacggaaa aacagaatgc tctccagatg
     2101 ctgatggaga aatattctcc agacggccat gatgcagcaa gtgagtacat caaccgcaat
     2161 attgaaagaa cagcggtcat tgcaattgaa atccagcata tcactgcaaa ggcagcaaac
     2221 taaatcagga cgcagaacaa aacctcctga tacatttttt gcagaggaga tctctaaaaa
     2281 acttcagagc aaatactatg ctatggattc gctgtatctg cgatatgcca ctcctgagga
     2341 tataccccaa atacttgggt tcatccgtga actcgccgag tacgaacaca tggaaaatga
     2401 ggttgtggca acagaagagc ttctccacga gtggctgttt gtcaaacaaa aagcagaggt
     2461 tctaatcggg gaaaccgagg gaaaagctgt cgggtttgca ctgttctttc ataacttctc
     2521 cacatttctc ggacgtgccg gaatctatct ggaggatttg tatgtgaagc cggagtttcg
     2581 cggaagaggt tttgggagag cctttcttgc acgacttgca gcaactgcgg tggagcgcgg
     2641 ctgtggcagg ctggagtggt ggtgtcttga ctggaaccgt ccgagcattg agttttacct
     2701 ttcgcttggc gctgtaccaa tgagcgactg gactgtgtac cgcatctgtg gcaacacgtt
     2761 gtctgaactg gcaaaaacgg atcggcagta gtccgacttg gcggaaatgc cacgtcgtct
     2821 ttatagtgtt gcaagaccaa agttacagaa gagattttct atgtttttga ttggtgaagc
     2881 actcgttggc gatggagcag aactcgctca catcgatctt atgatcggcg acaagatggg
     2941 gccggtcgga accgcttttg caaacggcct gacccagctc tccgccggac acactccgct
     3001 gcttggtgtt atccgcccga accttctgcc aaaaccggca gttctgatcg ttccgaaggt
     3061 tacgctgaag cacaccgaac aggtaaccca gatcttcggc ccggcacagg ccgcagtctc
     3121 caaagcagtt gccgatgcac tggaagatgg cgtctttacc ggtatggatg tggatgatgt
     3181 ggtcattgtt gcaagtgttt tcatctctcc ggaggcaaag gacttcaaca agctgtaccg
     3241 cttcaactac ggagcaaccc gccttgcact ctcccgtgcg cttgacaagt tcccggacac
     3301 tgcaaccgtc ctgaaggaga aggacagagc agcccacgca gttatgggat tcaaagttca
     3361 gcggctctgg aacccgccgt atctgcaggt cgcaatggac cttgtcgata tgaagcaggt
     3421 ggagcgtgtc ctgcgtgagg ttccgcagaa cgatcacgtc atctttgagg caggaacccc
     3481 gcttatcaaa cagttcggct tacatgtgat tggtgagatc cgcaaaatcc gcccgaactg
     3541 cttcattgtt gcagatttga agaccctgga caccggcaac cttgaggccc gtatggtctc
     3601 caacgccggc ggcgatgcag cagtggtctc cggccttgca ccgatcgaga cgatcgcagc
     3661 cttcatcaaa gaggcaaaga agtgcggaat ctatgcaatc atcgatatgc tcaatgttgc
     3721 agaaccggca aagctgatcg agaaccttgc aaagatggga ggagcagcgc ttcttccgca
     3781 gtatgttgag atgcaccgcg caatcgacaa agaggcaagc ggcgagtaca gctggggcga
     3841 catcaaga
//
LOCUS       DALUAJ010000061         2384 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_10895,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000061 DALUAJ010000000
VERSION     DALUAJ010000061.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10895"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="O0X37_01390"
     CDS             complement(<1..1111)
                     /locus_tag="O0X37_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HJK61614.1"
                     /translation="MHVLFPTGRQSEAALRSALDGTDAFTYSVVPTGEIASFLSPGIL
                     SRLLSENHYDLVVVSGMCTAEFAEVEQSSGVPVRKGTRHAADMRLCIPLIVSGRLSAT
                     VPADSLLSDVRAREAAERLAEMEDSAEASFVLRGVKFGGTSRIKIIHEIMDAHRHPSL
                     RKAVLAAAAGGADVVDLGFGFDASPEDVRRCFTEIADLDLPLSVDTVDPDLIEAALFR
                     CDMIFSLTSETIPQLAKQISASGAAAVLIPKTASLTDTVALAEKFGLSKILADPLLQP
                     PLSGMVGSLAGYLPEFGCPKVLGCVNVVEMVDADSPGMCALLAAAAEECGVAAVLVSE
                     HSDKTQGATQEMRRAVEMTALSRGRPYPKDVGKDVL"
     gene            1209..1928
                     /locus_tag="O0X37_01395"
     CDS             1209..1928
                     /locus_tag="O0X37_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HJK61615.1"
                     /translation="MVLLKCDLHVHTNASRDGESSVEDVLAAAVKAGLDAIAITDHDT
                     TEGSLCALAAQNPGVLIIPGIEVSTRQGHLLVLGTTKVLAPKQDVLKTIAEAKALGAV
                     TIVPHPFHRWRHGVGLRCKDALREADAVEALNSRYIIGTANQKAAKMAKKYGRPVTAG
                     SDAHNCKFVGFGITEIDAEARSVAAVLDAIRAGRISCTCKKTPLRTYTRQSWDNTVRK
                     VRRRVPQFRHRPRRRVVHRKK"
     gene            1925..>2384
                     /locus_tag="O0X37_01400"
     CDS             1925..>2384
                     /locus_tag="O0X37_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HJK61616.1"
                     /translation="MKIAFAVGYKGDNFAGSQAQPNKRTVEGEFVAAGIGLGLFADAK
                     EAHFRISGRTDKGVSARRQIVSITTDHPEKAIDALNFWLPDDIWCLGAAEVSPDFYPR
                     YAVTSRTYRYYFPYAADVAAMDAAARQFVGKHDFTRFSKMEEGRDQNRTVT"
BASE COUNT          632 a          622 c          673 g          457 t
ORIGIN      
        1 taagaacatc ctttccgaca tccttgggat acgggcgtcc gcgtgaaagt gcggtcatct
       61 caacggcacg ccgcatctcc tgggttgctc cctgcgtttt atcggagtgt tcggagacga
      121 ggacagcagc aacaccgcat tcctccgcag ccgcagcaag cagggcacac atacccggac
      181 tgtcagcatc aaccatctca accacattca cacagccaag aactttgggg catccgaact
      241 cgggcaggta tccggcaagg gatccgacca ttccggagag cggcggctga agcagcgggt
      301 cggcgagaat ttttgaaaga ccaaactttt ctgcaagtgc aacggtatct gtaagagagg
      361 cagtcttggg aatcagaaca gccgcagccc cggaggcaga aatctgtttt gcaagttggg
      421 gaatggtctc ggaagtcaga gagaagatca tatcgcaacg aaacagtgcg gcctcgataa
      481 gatccggatc aacggtgtcg accgagagag ggagatcaag atcagcgatc tccgtaaaac
      541 atcggcggac atcttctggt gatgcatcaa atccgaagcc aagatccaca acatcggcac
      601 caccggcggc tgcggcaaga acagctttgc gcagagacgg atgccggtga gcatccataa
      661 tctcatggat gattttgatc cgagaggtcc ctccgaattt aacgccgcga agtacaaagg
      721 atgcctcggc ggaatcttcc atctctgcta atcgttcagc cgcttccctg gctcgcacat
      781 cgctaagaag tgaatccgca ggaacggttg cggaaagacg tccggaaacg atcagcggaa
      841 tgcaaagccg catgtccgcg gcatgccggg tacctttgcg gaccggaaca ccagaggatt
      901 gttccacttc cgcaaactcg gcagtacaca tccctgagac cacaacaaga tcgtagtggt
      961 tttcggagag cagacgtgac aaaattcccg gtgaaagaaa tgaagcgatc tctccagtcg
     1021 gcacgacaga gtaggtaaat gcatccgtac cgtcaagtgc ggagcgcaga gccgcctcac
     1081 tctggcggcc ggtggggaag agaacatgca tatgatttct tattgggtcg gcgaacccga
     1141 aatagttagg gtttttccac agcaggaatg ttaatctgca atgaaagaga accccctaag
     1201 tacgcgaaat ggttcttctc aaatgtgatc tgcacgtcca tacaaacgca tcgagggacg
     1261 gagaaagttc ggtcgaggat gtacttgcag cggcagtgaa agcggggctt gacgcgattg
     1321 ccatcaccga tcacgacaca acagaaggat cactttgtgc gcttgccgca caaaatccag
     1381 gggttctgat cataccggga atcgaggttt caacacggca gggacatctg ctggttctcg
     1441 gaacaacaaa agtacttgca ccaaaacagg atgtactgaa aacaattgcc gaggcaaaag
     1501 ccctcggggc agtaaccatt gttccccacc cattccaccg atggcggcac ggagtaggcc
     1561 tccggtgcaa agatgcgttg cgggaagccg atgccgttga agcactgaac agccgctata
     1621 tcatcggaac ggcaaaccag aaagcagcaa agatggccaa gaagtacgga cggccggtga
     1681 ctgcgggatc cgatgcccac aactgtaagt tcgtcggatt cggtataacg gagatcgatg
     1741 ccgaggcgag atcagttgcg gcagttctcg atgccatccg ggcgggacgc atctcctgca
     1801 cctgcaaaaa gacgccgctc cggacgtaca cacggcagtc atgggacaat acggttcgca
     1861 aagtacgcag acgcgtaccg cagttccgcc accgaccgcg gcggcgtgtg gttcaccgga
     1921 aaaaatgaag atcgcatttg ctgtcggata caaaggggac aactttgccg gatctcaggc
     1981 acagccgaac aaacggacag tggaagggga gttcgttgca gccgggatcg ggcttgggtt
     2041 gtttgccgat gcaaaagaag cacattttcg catctcggga agaacggaca agggagtgtc
     2101 cgcacggcgg cagattgttt caatcacaac ggatcatccg gaaaaggcga tcgatgccct
     2161 caacttctgg ctgccggatg acatctggtg ccttggagca gctgaggtat cacctgattt
     2221 ttatccccgt tatgcggtta cttcgcgaac ctaccggtac tattttccct atgcagcaga
     2281 cgttgctgca atggatgcgg cggccagaca gtttgtggga aaacatgact ttactcggtt
     2341 ttccaaaatg gaagagggac gcgatcagaa ccggacagta acaa
//
LOCUS       DALUAJ010000062         5059 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_11456,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000062 DALUAJ010000000
VERSION     DALUAJ010000062.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11456"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(70..849)
                     /gene="rsmA"
                     /locus_tag="O0X37_01405"
     CDS             complement(70..849)
                     /gene="rsmA"
                     /locus_tag="O0X37_01405"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HJK61617.1"
                     /translation="MKAPKDQHFLVDADAVALIADTVPVSDRKVLEIGPGGGVLTDAL
                     LQRGALVRAVELDETLIPNLNRRFSEQIKSGQLEIIRGDASKVPLPEFELVVANLPYS
                     ISSKITFRLLEIGFESAVLMYQLEFGKRMIAPPGNSEYGRLSVMAQTYAEVEMVLELP
                     PEAFSPPPEVWSIVVKITPHEPPIPICDRKVHATLVRELFSHRRKTIRNGLRGMRSIY
                     GEQPVTNLIATLPDELLEMRPEMLSITDFIDLANRLSALIP"
     gene            complement(846..1424)
                     /locus_tag="O0X37_01410"
     CDS             complement(846..1424)
                     /locus_tag="O0X37_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF655 domain-containing protein"
                     /protein_id="HJK61618.1"
                     /translation="MPPKTERADKKEVEAIVLDFLQWGYADDKRPLNQREPVILAVGT
                     DQFKLLELIPKRNYAINLHDKVYIGDGERKVVERVKRRISYDELTNTAKGELEPVISM
                     IIAETEPRFVKFYNEAVPISLKLHMLNLLPGFGKKTLTDTLTERQKKPFESFEDIRSR
                     VKTLAKPEKFILERIMLELENPDEKYHLFTSK"
     gene            complement(1439..1810)
                     /locus_tag="O0X37_01415"
     CDS             complement(1439..1810)
                     /locus_tag="O0X37_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase Rpb4 family protein"
                     /protein_id="HJK61619.1"
                     /translation="MKVKKVISEEMMSLPELREVLIAIRDQRAGGDSETETSTRTMSY
                     ELRKSIDHADSLGKCDPATAKVLVDELNGLEKIRPEIAYRIVNIMPESRDELRAIYAK
                     ERYTLLPEDLDQILDILRQHA"
     gene            complement(1853..2146)
                     /locus_tag="O0X37_01420"
     CDS             complement(1853..2146)
                     /locus_tag="O0X37_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21e"
                     /protein_id="HJK61620.1"
                     /translation="MAKHNGIKKRTRYKLQKGLRERGMPNVTSVIQHFEEGQKVHIVI
                     EPSVQKGMPHPRFHGKTGTVLGQRGRAWLLEIKDGNATKVVISRPQHLKAQKY"
     gene            complement(2149..3390)
                     /locus_tag="O0X37_01425"
     CDS             complement(2149..3390)
                     /locus_tag="O0X37_01425"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0031119 - tRNA pseudouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(54/55) synthase Pus10"
                     /protein_id="HJK61621.1"
                     /translation="MTDILEKVHQILEYGDICDHCLGRLFAKRSFGLTNEQRGHALRV
                     AHALAYNIPFVPYEKGTCWICNDLFDTVPAWAEKVATAMQDIEYSTFVIGTRVPPMMS
                     ESEEMIWSDLSLEQPEPIKSEMNREVGKAVSALTGKRGDPKNPEITAVLNIADDCVEL
                     QIASIYFYGRYLKYERGIPQTHWDCRACRGKGCEKCNFTGKQYPTSVEELIAEVPKQM
                     FAAENGVLHGSGREDIDALMIGTGRPFVMEMQNPKIRNVNLLELESAINTFAAPRVQV
                     KLERWSDKKTVEMLKSHKGHKTYRILVDIDDSISLETVQNAVSKLKGALIHQRTPERV
                     AHRRADLIRERRVVDIECLGIEDSLFRIEVVGEGGLYIKELVSGDGGRTTPSLAEILA
                     VPAKVVALDVVQVDGINNGDE"
     gene            complement(3387..3956)
                     /gene="trmY"
                     /locus_tag="O0X37_01430"
     CDS             complement(3387..3956)
                     /gene="trmY"
                     /locus_tag="O0X37_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (pseudouridine(54)-N(1))-methyltransferase
                     TrmY"
                     /protein_id="HJK61622.1"
                     /translation="MLRFAILGHKATTSPDFSLNDMPGGAGRMDVLCRCINSSFFLSH
                     DLRRNTECYLILEGGEPHKILRFSGETIRSLNPDERSAGALIKKGLSTPAEPEFKKAA
                     DGIAIRSAGLAELLQEFSFAVLDENGEDIREVETLPETFILSDHMNFTEEEEELLKDL
                     PRYSVGPRVLHADHTITVILNEFDRREIS"
     gene            4149..5057
                     /locus_tag="O0X37_01435"
     CDS             4149..5057
                     /locus_tag="O0X37_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61623.1"
                     /translation="MQKKHILMILVICLLAAGISAAPAAAREVSSGSTIFVYEDGLNF
                     TGDLSGAAQLQYKSNNNILNMISVQDPDNFNLLASSVGDYTGSWDVINSSGTKLGTVL
                     IYYPDLSIDIVLAKDGVSSVQQWGFLDTEEYKIKISSPHVGPSGIGAKVNVIFKGADG
                     SETTYASGNSFADIDMNSVQVLTEASFLPKDLGKTTTIYAEWSSPTSFHNYAATSNSI
                     TMEYNDVTDPGVSITTNPTPTVTTSTPSVTVTTIPPTTVATTPVPIETTSEPTTEEPA
                     PSPTKSSLPAFLAPFSLLGVLILFRR"
BASE COUNT         1146 a         1309 c         1166 g         1438 t
ORIGIN      
        1 ctgcaagggc agcgcggatc agcaggtgtg tatcctctgc cgggaaatag atctgcgagg
       61 tatccggtat catggaatca gtgcagagag tctgtttgca agatcaatga aatctgtgat
      121 cgataacatc tcggggcgca tctccagcag ctcgtctgga agagttgcaa taagatttgt
      181 caccggttgt tctccgtaga tgctcctcat gccgcgcagt ccgtttctga ttgtcttcct
      241 ccggtgggag aacagttctc tgacgagtgt tgcgtgaacc ttccgatcac aaattggtat
      301 cggtggttca tgtggtgtga tcttcaccac aattgaccac acctccggtg gcggggagaa
      361 tgcctcagga gggagttcca gaaccatctc cacctccgca tatgtctgtg ccatcaccga
      421 gagtcttcca tactcactgt tgcccggagg cgcaatcatt cgtttcccga actccagctg
      481 gtacatcagt actgctgatt caaatccgat ctccagtagc cggaaggtga tttttgagga
      541 gattgagtac ggcaggtttg cgacaaccaa ttcaaattcc ggcagaggta cttttgatgc
      601 atccccgcga attatttcca actggcctga ttttatctgc tcggaaaaac gtcggttaag
      661 gtttggtatc agggtttcgt ccagctcaac cgcccggaca agtgcaccac gctgtaacag
      721 agcatctgtc agcacgccgc cgccgggtcc gatctcaagg acctttctgt cggataccgg
      781 aacggtgtct gcaatcagcg caacagcatc tgcatctacc aaaaaatgtt gatctttggg
      841 agccttcatt ttgacgtgaa gagatgatac ttctcgtccg gattttccag ctccagcata
      901 atccgttcaa gaataaactt ctccggcttc gcaagcgttt ttacccggga gcggatatcc
      961 tcaaagctct cgaacggttt tttctgccgc tcggtaagtg tatcagtcag agtcttcttt
     1021 ccaaaacccg gaaggagatt taacatgtgt agtttcagac tgatgggcac cgcctcgtta
     1081 tagaacttta cgaaccgcgg ttcggtctct gcgatgatca ttgagatcac cggctcaagt
     1141 tcaccttttg cggtattggt cagttcatca taggagatcc ggcgctttac ccgttcaaca
     1201 accttccgtt caccatctcc gatgtacact ttgtcgtgca gattgatggc gtagttccgt
     1261 ttaggaatca gctccagtag tttaaactgg tcggtaccga ccgcaaggat caccggctca
     1321 cgctggttta acgggcgttt gtcatcagca tatccccact gaaggaaatc caatacgata
     1381 gcttcgactt cctttttgtc ggctctctcg gtttttggag gcattatctc tacctcactc
     1441 acgcatgctg acggagaatg tcaaggatct gatccagatc ctccggaaga agtgtgtatc
     1501 gctcctttgc gtaaattgcg cgaagttcgt cacggctctc cggcatgata ttgacaatcc
     1561 ggtaggcaat ctccggtctt atcttttcaa gaccgttcaa ctcatcaaca aggacttttg
     1621 cggttgcggg atcacacttt ccgagactgt cggcatgatc gatactcttg cgcagctcgt
     1681 acgacatcgt ccgtgtagac gtttccgtct cgctgtctcc gccggcacgc tgatcacgaa
     1741 ttgcgatcag tacctcacga agttccggca gcgacatcat ttcttcgctg ataacttttt
     1801 tcactttcat gcctgtgact cctgaaataa atgtaaaatt agtatttggg ggttaatatt
     1861 tctgcgcctt gaggtgctgt ggacgggaga tgaccacttt ggttgcattg ccgtctttga
     1921 tctcaagaag ccaggcacgg ccgcgctggc caaggaccgt accggtcttg ccgtggaaac
     1981 gcgggtgcgg catgcctttc tgcacgctcg gctcaatgac gatgtgaacc ttctgaccct
     2041 cttcgaagtg ctggataacc gacgttacat tcggcattcc ccgctcacga agacccttct
     2101 gaagcttata ccgtgtccgt ttcttaatac cattatgttt tgccattttt actcatctcc
     2161 attattaatt ccgtcgacct gcaccacgtc taacgcgaca actttggcgg ggacggcgag
     2221 gatttcagcg agacttggag ttgttctacc tccgtctcct gatacgagtt ctttgatata
     2281 gagtcctcct tctcctacaa cctcgatgcg gaagagggaa tcctctattc cgagacactc
     2341 gatatcaacg acccttctct ctctgataag atccgctctg cgatgtgcta cgcgttcagg
     2401 cgtgcgctgg tgaatcaaag ctccttttag ctttgatacc gcattttgaa ccgtttcaag
     2461 agagatactg tcgtctatat cgaccagaat cctgtatgtt ttatgccctt tgtgtgattt
     2521 aagcatttcc accgtctttt tatcggacca ccgttccagc tttacctgga cgcggggtgc
     2581 ggcgaatgta tttatcgctg attccagttc caggagattt acatttcgga tttttggatt
     2641 ctgcatctcc atcacaaacg gtctgccggt acctatcatc agtgcatcaa tgtcttctct
     2701 gccggatccg tgtaatactc cgttttccgc agcaaacatc tgttttggaa cctctgcaat
     2761 cagttcttca acggaagttg gatactgttt gccggtgaaa ttacactttt cacatccctt
     2821 tccccggcat gcgcggcagt cccagtgcgt ctgtgggatt cctctttcat acttgaggta
     2881 tctgccgtag aagtagatgg atgcaatctg cagctccaca cagtcatcag cgatgttcag
     2941 tacagctgtt atctcagggt tctttgggtc tcctcgtttt ccggtcagag cggatactgc
     3001 tttacccacc tcacggttca tttccgattt aatcggttcg ggctgttcca gagaaagatc
     3061 cgaccagatc atctcttctg attcgctcat cattggcggg acgcgtgtac ctatcacaaa
     3121 ggtactgtac tcaatgtcct gcatggcggt tgccactttt tctgcccatg caggtacagt
     3181 gtcgaagaga tcgttacaga tccagcaggt acctttctca tagggaacaa acgggatatt
     3241 atatgcaagg gcatgggcaa cccgcagtgc atgtccgcgc tgttcattcg ttaggccaaa
     3301 cgatcgtttt gcaaacagtc ttcccaaaca gtgatcacag atatcaccgt actccagaat
     3361 ctgatgtact ttctccagaa tatccgtcat gatatctccc tccggtcaaa ctcatttaag
     3421 atcactgtaa ttgtgtgatc agcatgcagt acccggggac cgaccgagta ccgcggaaga
     3481 tctttcagta gttcttcctc ctcttcggtg aagttcatgt gatcgcttaa gatgaaagtt
     3541 tccggcagtg tctctacttc ccggatatcc tcaccgtttt catcaaggac tgcaaaggaa
     3601 aattcctgta aaagttctgc aaggcctgct gatcgaattg caattccgtc cgcggctttt
     3661 ttgaactctg gttctgcagg agtactcaat ccttttttga tcagtgcacc ggcacttcgc
     3721 tcgtcagggt tcagtgagcg gatcgtctct ccggagaacc ggagtatctt gtgcggctcg
     3781 cctccttcaa ggattagata acactctgta ttacggcgga gatcgtgact gaggaagaat
     3841 gaactgttga tgcagcgaca gagaacgtcc attcgtccgg cacctcccgg catgtcgttt
     3901 aaggagaagt ccggggatgt tgtagccttg tggccgagga ttgcaaaccg gagcatgtga
     3961 gaatatacat tggctgttaa tcggcataag acgttcgccc ggtgggtttg ttccgaattc
     4021 ttttttggtg tatttgcaga gttagtgaag tgattgctgt agagaggtac tttctgttgc
     4081 tctttttctc cacaaataat ctatagcgtg agagcccaca tacaccttat acgtttgagg
     4141 agtattttat gcagaaaaaa cacattctta tgatcctcgt aatctgcctt cttgctgcag
     4201 gtatttccgc agctccggca gctgcccgtg aagtttcctc cggcagtact atctttgttt
     4261 acgaggatgg gctaaatttc accggtgatc tttctggtgc tgcgcaactg cagtacaagt
     4321 caaacaataa tatcctcaac atgatctctg ttcaggatcc tgataatttc aacctgctcg
     4381 catcttctgt tggtgattac accggttcat gggatgtcat taacagttcg gggacaaagc
     4441 ttggtacagt tctgatctat tatcctgatc tctccattga catcgttctt gcaaaagacg
     4501 gtgtttcttc agtgcagcag tggggattcc ttgataccga ggagtataag atcaagatta
     4561 gttcaccgca tgtgggtcca agcggcattg gtgccaaggt aaatgtcatc ttcaaagggg
     4621 cagacggttc tgaaactaca tatgcctcgg gcaatagttt tgctgacatc gatatgaaca
     4681 gtgttcaggt tctgacagag gcatccttcc tgccaaagga tctgggcaag accaccacga
     4741 tctatgctga gtggagttct ccaacttcct tccacaacta tgcggcaaca tccaactcca
     4801 tcaccatgga gtataatgat gtaactgatc ctggtgtgag catcaccact aacccgaccc
     4861 caactgtaac tacatcaact ccctctgtta ctgtaacgac cattccccca acaacagtag
     4921 caacaactcc agtacccatt gaaacaacat cagaaccgac cactgaggaa cctgcacctt
     4981 ctccaacaaa atcttctctc cccgctttcc tggctccgtt ctctctcctt ggagtactca
     5041 tcctgttccg gagataaaa
//
LOCUS       DALUAJ010000063         3722 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_11560,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000063 DALUAJ010000000
VERSION     DALUAJ010000063.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11560"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="O0X37_01440"
     CDS             complement(<1..849)
                     /locus_tag="O0X37_01440"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presenilin family intramembrane aspartyl
                     protease"
                     /protein_id="HJK61624.1"
                     /translation="MSEFSIRSLVPYAGMLFLMLATGVLSLLLIYPVTEAGLGAFEDP
                     DSIANPFVFLFIMLVFTAILLLLIKWKAQTVISAIIGICLALVIYYVVSSLLFSYVPM
                     LPASPIGVVVAVLVVLLLWYRPEWYVINVFGILVSAGCASIFGVSLSVIPVMILLILL
                     IVYDAVSVNRTKHMLTLADGVLRQKMPIMFIVPKTRGYSYRKTGFSLQDKKEERGAYM
                     IGMGDMIMPAILVVSAQVYAGGEGILSIAGIALPALGALVGGIAGLCLLMIPLNSGKP
                     QPGLPYI"
     gene            892..1221
                     /locus_tag="O0X37_01445"
     CDS             892..1221
                     /locus_tag="O0X37_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61625.1"
                     /translation="MNVRRESLPGILLEILLVIVMAVSGLAIIIRLWQDAIVAIGVEL
                     LIISVGVLLLSILFRLRRLEEQSAARERIMRSNLEDLGRQMIQRQDATSQKVVEAVES
                     IKSRMYR"
     gene            1231..2232
                     /gene="fen"
                     /locus_tag="O0X37_01450"
     CDS             1231..2232
                     /gene="fen"
                     /locus_tag="O0X37_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease-1"
                     /protein_id="HJK61626.1"
                     /translation="MGVALRPLIADYKETLGWDQLGGTAAIDAYNALYQFLSGIRQPD
                     GTPLMDDDGHITSHLSGLLFRTANLVEKNITPVYVFDGKPPAFKEATLAERRIIRENA
                     KDSWERAVKEGDEETARKYAMASSKVDAYVINSSKELLTALGIPWIQAPEEGEAQSAY
                     MAAAGDVTYAVSQDYDSLLFGTPKLLRNLTVSGKRRMHGKYVPVYPERLILEDVLNGL
                     GMSRVELIAAALLIGTDYNSGVKGVGPKTAVKIVRDGKFAERITESENAADPEDLICY
                     FLDPPVEKNYTIAKRSPVQEKVFEILCEEHGFSEERVSAGLERLGAKKGQATLDAWF"
     gene            complement(2235..2612)
                     /locus_tag="O0X37_01455"
     CDS             complement(2235..2612)
                     /locus_tag="O0X37_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HJK61627.1"
                     /translation="MTDCPFCNPDDILLENDLAFAKYDLYPVSPGHVLVVPKRHTASW
                     FDLTPAEQTAMFALANQAKKLLDERYAPAGYNLGVNCGEAAGQTIMHVHLHIIPRYCG
                     DVPNPRGGIRAVIAAKQDYTHWV"
     gene            complement(2620..3483)
                     /gene="artA"
                     /locus_tag="O0X37_01460"
     CDS             complement(2620..3483)
                     /gene="artA"
                     /locus_tag="O0X37_01460"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase A"
                     /protein_id="HJK61628.1"
                     /translation="MLEQLIEAVVIFSCIAFLLFLIPGKHQKYVASVGWITMEAVLLL
                     MFPFFFEEGNFVYPTIAIVAIPCVYITVKRLFMDDYYIRRLTYAASIAYLVYAPFAFI
                     PAVGNWLISVVVELVAVVLTAIGTPFTMADWNIFMGEVLPGNTFGYQVEVILGCTGIQ
                     AIAILLGVVAIIPSSWKRKLGLFFLVTIPIFAVNIFRNAYVVTAYTQQWYPWFQEWFP
                     GPEMVGYASFFWAHNVFCELLALAVLVVIAYLLFRLNPTMVAVLREIGVVYYDELTAM
                     RDRLFGKKATE"
     gene            3596..>3722
                     /locus_tag="O0X37_01465"
     CDS             3596..>3722
                     /locus_tag="O0X37_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor S"
                     /protein_id="HJK61629.1"
                     /translation="MFCPKCGKLMKSKDGKLTCAKCGYAEEISSAEKEHMKKVAYM"
BASE COUNT         1002 a          903 c          975 g          842 t
ORIGIN      
        1 aatatatgga agacccggct gcggttttcc tgagttcagc ggaatcatca gcaggcacaa
       61 acctgcaatt cctccaacca gcgcgccgag tgcgggaagt gcaatccctg ctattgagag
      121 gattccttcg ccacctgcat agacctgtgc ggaaaccaca agaatcgcag gcatgatcat
      181 atctcccatt ccgatcatgt aggccccgcg ttcctctttt ttgtcctgca gggaaaagcc
      241 tgtctttctg tacgagtatc cgcgggtttt ggggacaata aacataatcg gcattttctg
      301 gcgaagaaca ccgtccgcaa gtgtcagcat atgttttgtc cggtttactg ataccgcatc
      361 atatacaatt aacagaatca agagaatcat caccggaatg accgaaagcg aaacaccaaa
      421 gatcgaagca caccctgctg aaacaagaat tccaaagaca tttattacat accattccgg
      481 tcggtaccag aggagaagta ccaccagtac cgcaactacc actccaatgg gggatgccgg
      541 gagcatcgga acataggaga acagcagtga ggagacgaca tagtagatca ccagtgcaag
      601 acagatcccg atgattgcac tgataaccgt ctgtgctttc catttgatca aaagcagcag
      661 aattgcggta aacacgagca tgatgaacag gaatacaaac ggatttgcga tcgagtccgg
      721 atcctcaaaa gctccaagcc cagcttctgt taccgggtaa atcagcagaa gcgacagcac
      781 ccctgttgca agcatcagga atagcatgcc agcgtagggt acaagagaac gaatcgagaa
      841 ttcactcatg tataggtaac ttaatatttt agcggtctcc ttagttaaaa tatgaatgtt
      901 cgtcgtgaaa gccttccggg aatcctactg gaaattctgc tggtaattgt catggcagtg
      961 tccggtcttg ccattattat ccggctttgg caggacgcaa ttgtcgcaat cggagttgaa
     1021 ctgctgatta tttccgtggg agtactgctg ctttcgatac ttttccgcct tcgccgtctt
     1081 gaagagcaga gcgcggcacg tgagcggatc atgcgcagca atcttgagga ccttggccgg
     1141 cagatgattc agaggcagga tgcaacctcg cagaaggttg ttgaggcggt cgagagcatc
     1201 aaaagcagaa tgtaccggta aggagatttc atgggtgttg cgctgcggcc gcttattgcg
     1261 gactataaag agacccttgg atgggatcag cttggcggta ctgcggccat tgatgcctac
     1321 aacgccctgt atcagttttt aagcggcatt cgtcagccgg acggcacacc gctgatggat
     1381 gacgacggcc atatcacgtc gcatctcagc ggtcttttgt tccgtaccgc aaaccttgtg
     1441 gaaaaaaata tcacgcctgt gtatgtgttt gacggaaaac ctccggcatt taaggaggct
     1501 acccttgccg aacgccgtat cattcgcgaa aatgcaaagg attcctggga acgtgcagta
     1561 aaagaagggg atgaggagac tgcacgcaag tatgccatgg catcatcaaa ggttgatgcc
     1621 tatgtcatca actcctccaa agaacttcta acggccctgg gaatcccgtg gattcaggcc
     1681 cctgaggagg gagaagcaca gtctgcctac atggcagccg caggggacgt gacttatgct
     1741 gtctcccagg actatgactc cctgctgttt ggaacaccga aacttctccg gaacctcacg
     1801 gtttcaggca agcgccgtat gcacggaaag tatgtgccgg tctatccgga gcggctgatt
     1861 ttggaagatg tcctgaacgg gcttggcatg agccgtgttg agttgatcgc agcagccctt
     1921 ctcataggga ccgactacaa cagcggtgta aagggtgtag ggccgaagac tgcggtaaag
     1981 attgttcgtg acggaaagtt cgccgagcgc attaccgagt cggaaaacgc cgcagacccg
     2041 gaggacctta tctgttactt ccttgacccg cctgtggaaa agaactacac cattgcaaaa
     2101 agatcccctg tgcaggagaa ggtgtttgag attctctgtg aagagcacgg cttctcggag
     2161 gaacgcgtgt ctgcgggact cgaacggctc ggtgcaaaaa agggccaggc gacccttgat
     2221 gcctggttct gaggtcagac ccagtgggta taatcctgtt ttgccgcaat taccgcacgt
     2281 attcccccgc ggggatttgg gacatcaccg cagtatcggg gaatgatatg cagatggaca
     2341 tgcatgattg tctgacctgc cgcctctccg cagtttacgc ccaggttgta cccagcaggc
     2401 gcatatcgtt catccagtag tttttttgcc tggttggcaa gtgcaaacat ggcggtctgt
     2461 tctgcgggtg tgagatcaaa ccaggatgct gtatgccgct tgggaaccac cagcacatgg
     2521 ccgggcgaga caggatacag atcgtacttg gcaaatgcca gatcattttc taggagaatg
     2581 tcatccgggt tgcagaaggg acagtcggtc ataaaaaaat tattctgttg cttttttgcc
     2641 aaacagacgg tcacgcattg cggtcagttc gtcataatac actactccga tctcacgaag
     2701 tactgcaacc atggtggggt tgagtctgaa gaggaggtag gcaataacga ccagcaccgc
     2761 aagggcgagg agttcgcaga acacattgtg cgcccagaag aaacttgcat agccaaccat
     2821 ctctggtcct gggaaccatt cctggaacca ggggtaccat tgctgggtat atgcggttac
     2881 aacatatgca ttgcggaaga tgttcactgc aaagataggg attgtcacca aaaagaacaa
     2941 tccgagtttc ctcttccagc tcgacgggat gatggccact accccgagaa gaatggctat
     3001 tgcctgaatt ccggtacagc caagaatcac ctctacctga taaccgaagg tgttgcccgg
     3061 cagaacctca cccatgaaga tgttccaatc cgccatggta aacggtgttc caattgctgt
     3121 cagaacaaca gctaccagtt ccaccaccac gctgatgagc cagtttccaa ctgccggaat
     3181 gaacgcaaac ggtgcatata ccagataggc aatggatgct gcgtacgtga gtcggcgaat
     3241 atagtagtcg tccatgaaca accgctttac ggtgatgtaa acacacggaa tggcaacaat
     3301 tgcaattgtt ggatagacga agtttccctc ttcaaagaag aaaggaaaca tcagcaggag
     3361 gaccgcctcc attgtaatcc acccgacact cgccacgtac ttctgatgct ttcctggaat
     3421 cagaaacagc aggaatgcaa tacaggaaaa gatgactacg gcttcgatca actgctccag
     3481 cataagaact ggttacactg ttagccgtaa tggatgaata gggttttgtt ggggttctgc
     3541 acctgcgggt atggtcagag tatataaggt tccaagacaa atatttaact ctatgatgtt
     3601 ctgtccgaaa tgcgggaaac tgatgaagtc caaagacggc aagctgacct gtgcaaaatg
     3661 cgggtatgca gaggagatca gctcagcaga gaaggaacat atgaaaaagg ttgcttacat
     3721 gc
//
LOCUS       DALUAJ010000064         3840 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_11665,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000064 DALUAJ010000000
VERSION     DALUAJ010000064.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11665"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            7..408
                     /locus_tag="O0X37_01470"
     CDS             7..408
                     /locus_tag="O0X37_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6e"
                     /protein_id="HJK61630.1"
                     /translation="MVDFKVVLSDPKTGLSYKIDATGAAAGALLGKKIGTEVDGAPFG
                     LNGYKITITGGSDKTGTPARPDLPGNGRRNLLLSDGFGFHAKHNGERKRKSQRGNEIA
                     ADFVQVNAKISTYGEKPIAEIFAPAEEATAE"
     gene            complement(549..1031)
                     /locus_tag="O0X37_01475"
     CDS             complement(549..1031)
                     /locus_tag="O0X37_01475"
                     /inference="COORDINATES: protein motif:HMM:NF021513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2240 family protein"
                     /protein_id="HJK61631.1"
                     /translation="MNELETTVAAPFRHGRKDRLSKMELVFYYVQDKRWMSQDQAKKL
                     IDLAAKRGLLLKEGDDNTYRCAESLGDIAIPLGFRPGDEIFSESSDEKDPIEALIEDI
                     ATATGRSRKDLAAEMQEIAEHFDNLLVPEASVVLLAKKYGVNTVAYIPALRAAMREGQ
                     "
     gene            complement(1028..1450)
                     /locus_tag="O0X37_01480"
     CDS             complement(1028..1450)
                     /locus_tag="O0X37_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61632.1"
                     /translation="MRRVSYQFPNRCDLEFEVDVPFSVFLNAIESLAGRQDNFRTDAG
                     PIKREVSVFLAHDRLGFEKIERGANISKTTTDAADILITLTDTLLRASGNVPLNDHRF
                     MIRTMERGARTEIFVTKSPSIDGFDAMDMKKMILGALS"
     gene            complement(1524..1901)
                     /locus_tag="O0X37_01485"
     CDS             complement(1524..1901)
                     /locus_tag="O0X37_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8e"
                     /protein_id="HJK61633.1"
                     /translation="MLWQGRSVRKSTGGRYHANRGKKRFEIGRSPADTTIGETRVKTI
                     RVTGGNTKVRALRCEYANVSDKKTGKVQKVKINSVAENAANPNYVRRNLMTKGAIIAT
                     DLGKARIVSRPGQDGVINAVLLE"
     gene            complement(2059..2706)
                     /gene="hypB"
                     /locus_tag="O0X37_01490"
     CDS             complement(2059..2706)
                     /gene="hypB"
                     /locus_tag="O0X37_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HJK61634.1"
                     /translation="MHHVDVHMEAEIYGANNRLAEHNAEHFKAHGVRAFDLLGAIGSG
                     KTSLIEQIVPELKKRNLNTAAIAGDVYGDDDFQRIVKTGIPAFNANTGKECHLDAHLV
                     HHALDHLDLDDIDVVFIENVGNMVCPTDFKLGAEKRVVVISTTEGDDVVNKHPMMFRD
                     CQIAVINKVDLAEAVGCNVERMIADIKRYNPEMVIIKTNLRKGEGVAEVVDAILA"
     gene            2940..3296
                     /locus_tag="O0X37_01495"
     CDS             2940..3296
                     /locus_tag="O0X37_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61635.1"
                     /translation="MNTFSKIAAFLLALSILAIPAMAVEEEEHLILVKSDPNNPDIVY
                     MTQITIPGHEEIPEHAELRPFFRDSDPEFAVAETDTTSYPVENVVISKETAIPDSPVD
                     IASYEKGEKYEYAAIP"
     gene            3385..3657
                     /locus_tag="O0X37_01500"
     CDS             3385..3657
                     /locus_tag="O0X37_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle subunit SRP19/SEC65
                     family protein"
                     /protein_id="HJK61636.1"
                     /translation="MAIRYLYPCYFNAELTRAKGRRVAKDIAVPSPNLAQVARAAKQC
                     GFTVLDEERDVAHPAYWFSREGRLRVEHEGSKEELLKKVAHKLSGK"
     gene            3661..>3840
                     /locus_tag="O0X37_01505"
     CDS             3661..>3840
                     /locus_tag="O0X37_01505"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HJK61637.1"
                     /translation="MYDFHCHTTMSDGELLPTELIRRMAVLGYSEMAIADHADFSNVR
                     ELICAEEAVKRSAELY"
BASE COUNT         1035 a          909 c          911 g          985 t
ORIGIN      
        1 aatacaatgg tagactttaa ggtcgtactg tcagacccga aaactggtct ttcgtacaaa
       61 atagatgcca cgggcgcagc tgcgggtgca cttctgggaa agaagatcgg caccgaggtt
      121 gacggcgctc cgtttggtct gaatggttac aagattacaa tcaccggtgg atcggacaaa
      181 accggaactc cggcacgccc ggacctccct ggtaacggca gaagaaacct ccttctctcc
      241 gacggatttg gtttccacgc aaaacacaac ggtgagcgga aaagaaagag ccagcgcggc
      301 aacgagatcg ctgctgactt tgttcaggta aacgcgaaaa tctccaccta cggcgaaaag
      361 ccgatcgcag agatctttgc ccctgctgaa gaagcaactg cagagtaaat ctctctcaat
      421 actttttctc ttcttctctt gatgtattca caggtaagat catttttccc tgcgaatcgt
      481 ctattcaaaa gcaaaagact atactggcag agtagagatt ccctgccacc atttctaagg
      541 aaaaacagtt actgtccctc gcgcattgct gcgcggaggg caggaatata ggcaacggta
      601 ttgacaccgt atttttttgc aaggagaaca acagatgctt caggaacgag aagattatcg
      661 aaatgttctg cgatctcctg catctcagca gcaagatctt ttctggatct gccggttgca
      721 gtggcaatgt cctcgatcag tgcctcgata ggatccttct catcggagga ctcggaaaag
      781 atctcatcac cgggacgaaa acccagtggt atggcaatgt ctccaaggga ttctgcacaa
      841 cggtacgtgt tgtcatcccc ctccttaaga aggagacctc gctttgcggc aaggtcaatt
      901 agtttttttg cctgatcctg gctcatccac cgtttgtcct gcacataata aaacaccagc
      961 tccattttac tgagtcgatc ttttctcccg tgccggaaag gtgctgcaac cgtcgtttcc
     1021 agttcgttca cgaaagtgcc ccaagaatca ttttcttcat atccattgca tcgaacccat
     1081 cgatagacgg ggacttcgtg acaaatatct cagttcgtgc tccacgttcc atggtacgaa
     1141 tcatgaaccg atgatcgttc aacggaacat tcccagaagc tctcagcaga gtatctgtaa
     1201 gggtgatcag gatgtctgct gcatctgtag tggttttgct gatatttgca ccacgctcaa
     1261 ttttttcaaa accaagccgg tcgtgcgcaa gaaaaacgga gacctcacgc ttgatgggac
     1321 ctgcatctgt tcggaagttg tcctgccgac ctgcaagtga ttcaatagcg ttcaggaaga
     1381 cagaaaacgg gacatcaact tcaaattcaa gatcacagcg gtttgggaac tgataggaca
     1441 ctcttctcat gcatcagata ttgggcactg atatggataa gtatattttg ctgggaacaa
     1501 gagaaaagaa aaaaatattt tgtttattcg agcaggactg cgttgataac gccatcctga
     1561 cccgggcggc tgacgatacg ggctttaccg agatcggttg cgataattgc gcccttggtc
     1621 atcaggttcc gacgaacata gttcgggttt gctgcatttt ctgcaacaga gttgatcttg
     1681 accttctgaa cctttccggt cttcttgtcg ctgacgtttg cgtattcgca gcggagtgcg
     1741 cggaccttgg tgttaccacc ggttactctg atggtcttta cacgggtttc tccaatggtg
     1801 gtatcagccg gggatctgcc gatctcaaac cgctttttgc cacggttggc atggtaacgg
     1861 ccaccggtgg actttcttac tgatcttcct tgccaaagca tgaattaata tctccaataa
     1921 ttaagtttct ctgattaaac gcccgaagaa gttacgcttt ccggacgggt gatcctgaac
     1981 cggaaggtcc agcctcagga tcatctcagt cttttccaga gtcctatatg tataggcttt
     2041 cccgatattt aaattccgtt aggcaaggat ggcatcaaca acctcggcca caccctcccc
     2101 tttacggagg ttggttttga tgatgaccat ttcagggttg taacgcttga tatcggcaat
     2161 catgcgctcg acattacagc cgactgcttc agcgagatcg accttattaa taacagcaat
     2221 ctggcagtca cggaacatca tcgggtgttt attgacaaca tcatcgcctt cggtggtgga
     2281 gatgacaact acgcgcttct ccgcgccgag tttgaaatca gtgggacaga ccatgttgcc
     2341 gacattttcg atgaagacca cgtcaatgtc gtcaaggtca aggtgatcaa gggcatggtg
     2401 aacgaggtgc gcatctaaat ggcactcttt tccggtgttt gcattgaaag ctggaatgcc
     2461 cgttttgaca attctctgaa aatcatcatc accatagaca tcaccggcaa ttgcagcagt
     2521 attgaggttg cgctttttaa gttccgggac gatctgttca atgagagagg tcttacctga
     2581 cccaattgct ccaaggagat caaatgcacg aacgccgtgt gctttgaagt gctcggcgtt
     2641 gtgttctgcg agacgattat tggcgccgta aatctcggcc tccatgtgaa catccacgtg
     2701 atgcatacaa gtactatatg gtgcgcgctg gtttatagat acactgttcc tcatgtgtat
     2761 gagtgcatca caccaatgag ataaaaaata tgatcagagt tacccagtgt ggatatgtag
     2821 taatcaagac aaaacaaaat gtaaagttat attaacattg aagatctact acttagtgtc
     2881 agagatactc tggcactcaa atacatatca taaccacaac catacaggag tacgctatca
     2941 tgaatacgtt ctcaaaaatt gcagcctttc tgcttgcgct gtccattcta gcaattccgg
     3001 caatggcagt ggaggaggaa gaacatctga tacttgttaa atctgaccca aacaatcctg
     3061 acattgtcta tatgacacag atcaccattc ccggacatga ggagatccct gaacatgcag
     3121 aacttcgtcc ctttttccgg gacagtgatc ctgagtttgc cgtagcagag acagatacaa
     3181 caagctatcc ggtagaaaat gttgtcatat ccaaagagac agcgataccg gactcacctg
     3241 tcgacattgc atcctatgaa aaaggagaaa agtacgagta cgcagctatc ccataaccgg
     3301 gaagagaaga cacattgatt caatattttt tcggcggact gccaattacc ttatagattt
     3361 tcacaccaat actgtatagt attcatggcg attcgatatc tctatccctg ctactttaat
     3421 gcagaattaa cccgagccaa gggtagacgt gtggcaaagg acattgctgt accgtcacca
     3481 aatcttgcac aggttgcgcg tgcagcaaaa cagtgcggct ttacagtact tgatgaggaa
     3541 cgagatgtgg cacacccggc atactggttc tcaagggaag gaaggctgcg ggtcgagcac
     3601 gaagggagca aagaagagct tctcaaaaaa gttgcccaca agttgagcgg gaagtaacca
     3661 atgtacgatt tccactgcca tactacgatg agtgacggag agctgttgcc aacggagtta
     3721 atacggagga tggcagtcct tggctattca gagatggcaa tcgcagatca cgcggacttc
     3781 tccaatgtta gggagttgat ctgtgcagaa gaagcggtaa agcgatcggc ggagctatat
//
LOCUS       DALUAJ010000065         2075 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_11760,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000065 DALUAJ010000000
VERSION     DALUAJ010000065.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11760"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="O0X37_01510"
     CDS             complement(<1..804)
                     /locus_tag="O0X37_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HJK61638.1"
                     /translation="MVMKRYLNQITADEAVTIIKSVPPHCSSRRIPVADAVGHRLSAS
                     LFALYSVPEVPVSAMDGFAVRSSETVGAGDQTPVVLRVFSRVNTGNVIPGEFDAVVRV
                     EDAWFEGEDPHEITVRKSVNPGTNVRPAGEDIKEGQLILPAGTVIRPFDVGAIAAYGI
                     THVNVSRVSVGILPTGTELIEPGTKPRPGQVVESNTVMAEAYLRQFGVDVVRYPPVVD
                     DPILIRGALERVLAENDVVIVSAGSSAGTKDFTASVIEEMGKLLFHGVAM"
     gene            complement(798..2000)
                     /locus_tag="O0X37_01515"
     CDS             complement(798..2000)
                     /locus_tag="O0X37_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HJK61639.1"
                     /translation="MRFLQLVSSAEAESTLLALGQPLPEELVPLAESVGRILSRRIVC
                     PEDIPGFHRSVKDGYAVRSVDTLGAGEQKPKLLKLTGRILMGQNASGMLEEKTTKYIP
                     TGGVMPIGADAVVMQESTELAGDMVLIKGPVSPGADVLRADEDFAKGEVVLEAGTKLT
                     AQATGVLAAFGIDPVPVVRRARVGILSTGNELVAPAATRSPGQIRDANSMLLISFMRE
                     AGAVPVFYGIIPDDASSLLPVLKTAASECDLVVVSGGSSKDERDVTAGVVAELGVIHV
                     HGVSIAPGKPTIIGSIGTTPVLGLPGHPAATYMITSVFAGPLLRRMSGEIFSQQVISA
                     PLSMSFPSEKGREDLVRVRILSDGRAEPILGKSGLLNTLVRSDGYIRVPAGLDGCEAG
                     EVVEVYLW"
BASE COUNT          546 a          667 c          442 g          420 t
ORIGIN      
        1 cattgcaact ccgtggaaca acaacttccc catctcctca atcaccgacg cagtgaaatc
       61 ctttgtcccc gcagaagaac ccgcagagac gattacaaca tcattctccg caagaacacg
      121 ctccaaggcc ccgcggatca gtatgggatc atccactact ggcggatacc gcacaacatc
      181 taccccgaac tggcggagat atgcctctgc catgaccgta ttactctcca ccacctgacc
      241 cggacgcggc ttggtccccg gctcaatcag ctcagtgccg gtcggtagaa taccaacaga
      301 aacacgggat acattcacgt gcgtaattcc ataggctgca atcgcaccca catcaaacgg
      361 ccggataact gttcctgcag gaagaatcaa ctgcccttct ttaatatcct caccagcagg
      421 cctgacattt gtacctgggt ttaccgattt gcgcaccgta atctcatgcg gatcctctcc
      481 ctcaaaccag gcatcctcaa cacgaacgac tgcatcaaac tccccgggaa ttacattacc
      541 cgtattcacc cgcgaaaaaa cacggaggac aacaggcgtc tgatccccag caccaaccgt
      601 ctctgatgac cgaactgcaa aaccgtccat tgcagaaacc ggaacttcag gaaccgaata
      661 cagtgcaaat aacgacgcag aaagccggtg acctaccgca tccgcaaccg gtattcggcg
      721 ggatgaacag tgcggcggca cactcttaat aattgtaact gcctcatcgg ctgtaatctg
      781 attcagatat cgtttcatta ccatagatac acctccacga cctctcctgc ctcacagccg
      841 tccagaccgg ctggaactcg gatgtagccg tcacttcgta ctagcgtatt taatagaccg
      901 gacttcccaa gaatcggctc tgcccggcca tcggaaagaa tccggacacg taccagatcc
      961 tcccttcctt tctctgacgg gaaactcatc gaaagcgggg cagaaataac ctgctgcgaa
     1021 aagatctcac cggacatcct ccggagcagc ggaccggcaa acacagacgt tatcatgtac
     1081 gtcgccgccg gatggcccgg aagtccgagt accggcgttg tgccaataga tccgataatg
     1141 gtaggtttgc ccggagcaat cgaaaccccg tgcacgtgaa tgacaccaag ttcggcaaca
     1201 acacctgctg taacatcacg ttcatccttg gaactaccac ccgatacaac caccagatca
     1261 cactctgagg cagcggtctt cagtaccggc agaagagatg atgcatcgtc tgggatgatg
     1321 ccgtaaaaaa ccggcaccgc acctgcttca cgcataaaag aaatcaaaag catactgttt
     1381 gcatccctta tctggcctgg gctccgcgtt gcggcaggtg caaccagttc attaccggtt
     1441 gagagaatac caacccgtgc ccggcgcacc actggtactg gatcaatgcc aaacgctgca
     1501 agaacaccgg tcgcctgagc tgtcagcttt gtaccggcct ccagtaccac ctcacccttg
     1561 gcaaaatcct catctgcccg aagcacatca gcccccggag aaaccggccc cttgatcaga
     1621 accatatcac cggccagctc agtactctcc tgcatcacca ccgcatcagc ccctattggc
     1681 atcacaccgc cggtcggaat atacttcgtt gttttctcct caagcatacc tgacgcattc
     1741 tgtcccatca aaattctgcc cgtaagcttc agaagtttcg gtttctgttc ccctgcaccc
     1801 aaggtatcca cagaccggac tgcatatccg tctttgaccg agcgatgaaa accgggtata
     1861 tcctctgggc acacaatcct gcgtgagaga atgcgaccga ccgactccgc aagagggacc
     1921 agttcttccg gcaaaggctg tcccaaagcc agcagggtac tttccgcctc cgcagaggac
     1981 actagttgta aaaatctcat acaaccttcc tgcacataat cccggagaca ggatactcca
     2041 tacctctccc agtggcttca aatatcatat ctaac
//
LOCUS       DALUAJ010000066         3583 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_11800,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000066 DALUAJ010000000
VERSION     DALUAJ010000066.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11800"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="O0X37_01520"
     CDS             <1..433
                     /locus_tag="O0X37_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833876.1"
                     /note="Directs the termination of nascent peptide
                     synthesis in response to the termination codons UAA, UAG
                     and UGA; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HJK61640.1"
                     /translation="IMDEKKEMTRFFKELIKENGCAAYGEESVRANLEAGAVDTLLLS
                     EKLRKARLTIECGNCGYQEVKTLQVEAGKKFKDLPFGHCPKCQSPLYLADETDIIEEL
                     TSLADISNTRVEIISDDFEEGAMLFSAFGGIAAILRYPTGM"
     gene            439..2115
                     /gene="argS"
                     /locus_tag="O0X37_01525"
     CDS             439..2115
                     /gene="argS"
                     /locus_tag="O0X37_01525"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HJK61641.1"
                     /translation="MYREYIHKVEHLLREITGENDVLLTDGGDHADIASTVAFALAKK
                     ERKNPSVIASEIVTKIIEHPAAAGLEVSAKGPYINFIFGGDYVAASVRAARHPDYGQL
                     PARTERVILEHTSANPNGPLHVGHIRNTVIGDTLVRVFRKAGYPVEAQYYLNDMGRQI
                     AIVAWGVKHLHYERLPNEKGDEFIVRHYVEANKIAKEKPEIEPEFDEMMEKIEAGDPE
                     TVKLFHDSVDICAEGIKQTLAAMNVHHDRFVRETTFLWNGDMQNVLARVEALPEAHHD
                     GQMMYLDLSDKGFGNRYVLRRSNGTSVYAARDLAFHLWKNAQCDRSIDVLGADHKLIG
                     AQLQTTLSMIGERPPEIVHFEFVSLPEGSMTTRGGVFITADELLAETKKRALEEVTIR
                     RPELGETERERIASAVAVGAVRYDIVKVSAEKSTVFDWKEALDFERQSAPYIQYAHAR
                     ACSILEKSKADGEFSENYTFTDPYEIALAKHLAQFPYVIEKVVVELRPHLLATYVRDL
                     ADLFNSFYRFAPVLKAEGSVRDARLTLVDASRNTLAAALDVLGIEALESM"
     gene            2468..3328
                     /locus_tag="O0X37_01530"
     CDS             2468..3328
                     /locus_tag="O0X37_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HJK61642.1"
                     /translation="MVTFSPAQVCVKVGSAGKTKCCLPAGNMFVRAFLAGAYIAMGAA
                     LATVGSTGVADVLGAGIAQFILGALFPIGLVLVVLTGAELFTGDAMFAPMAVLQGHTG
                     LKGLIYLWIVVYIGNLIGSLFMAFLVSYGPYTAWDAAGVATATAFGTRAIAIATAKVS
                     YFGPMGILSVFFKGILCNWLVCLALFLGLAADEVVSKILAIWFPIMAFVATGFEHCVA
                     NMYFIPAGIITNMIANGGADTAVLNWGTMWTNNIIWSTLGNIVGAVIFMAVIYYYCYK
                     SEICALCETK"
BASE COUNT          882 a          825 c          884 g          992 t
ORIGIN      
        1 catcatggac gaaaagaaag agatgacacg gttcttcaaa gagctcatca aagagaatgg
       61 atgtgcggca tatggtgagg aaagtgttcg tgcgaacctt gaggcgggag ccgttgacac
      121 tcttcttctt tcggagaagt tacgcaaagc ccggcttacc attgaatgtg gtaactgcgg
      181 ttatcaggaa gtaaagactc tgcaggttga agcagggaaa aagttcaagg atcttccgtt
      241 tggtcactgt ccaaaatgtc agtctccgct ttatcttgca gatgagacag atatcattga
      301 ggagttgacc agccttgcgg atatctccaa tacccgtgtt gaaatcatct ctgatgactt
      361 cgaggagggt gccatgctct ttagtgcatt tggtggtatt gccgcaatcc tgcgctatcc
      421 aacggggatg taacaatcat gtatcgggaa tatattcaca aagttgaaca ccttctccgt
      481 gagatcacgg gagaaaatga tgtactgctc acagacggcg gagatcatgc agacattgcc
      541 tcaacggttg catttgccct ggcaaaaaaa gaacggaaga atccaagcgt aattgcatct
      601 gagattgtta ccaagattat tgagcatccg gctgccgcgg gtcttgaggt ttctgcgaag
      661 ggtccctata tcaacttcat ctttggaggg gactatgtag ccgcatccgt tcgtgctgct
      721 cggcatccgg attacggcca gcttccggca agaaccgaac gggttattct tgaacacacc
      781 agtgccaacc cgaatggtcc tttgcatgtt ggtcatatca gaaacactgt cattggagat
      841 acgctggtcc gtgtattccg caaggccggg tacccggttg aagcgcagta ctacctgaat
      901 gatatggggc gccagattgc aattgttgcg tggggtgtca aacacctcca ctatgaacgg
      961 ctgccaaatg aaaagggtga tgagttcatt gtgcgccatt atgtcgaagc gaacaaaatt
     1021 gcaaaggaaa agccggagat tgaacctgag tttgatgaga tgatggaaaa gatcgaagca
     1081 ggcgatccgg agacggttaa acttttccac gactcagtgg atatttgcgc tgagggtatc
     1141 aaacaaactc ttgctgcaat gaatgtacat cacgatcggt tcgttcgtga gactaccttc
     1201 ctctggaatg gcgatatgca gaatgttctt gcaagagtcg aagctcttcc cgaggcacat
     1261 cacgacgggc agatgatgta tcttgatctg tcggacaaag gttttggaaa ccgttatgtg
     1321 ctgcgcagaa gcaatggcac ttctgtttat gcggcacgtg atcttgcatt ccatctctgg
     1381 aagaatgctc agtgtgaccg gagtatcgac gtccttggtg ccgatcacaa actgatcggt
     1441 gcacagctcc agacgacact ttcgatgatt ggtgaacgcc ctccggagat tgtacacttt
     1501 gagttcgttt ctctccctga aggatcgatg accacccgtg gcggtgtctt catcactgcc
     1561 gatgagcttc ttgccgaaac caaaaaacgg gcacttgaag aggttaccat tcgtcgtcct
     1621 gagcttggtg agaccgagcg cgaacggatt gcatcagctg tggcggttgg tgctgtgcgg
     1681 tatgatatcg ttaaagtttc tgcggagaaa tcaacggtgt ttgactggaa agaagcactt
     1741 gattttgaac ggcagagtgc accctatatc cagtatgcac atgcccgtgc ctgttctatt
     1801 cttgaaaagt caaaagccga tggcgaattt tcagaaaact atacctttac tgatccctat
     1861 gaaattgcac tggcaaaaca tcttgcacag ttcccgtatg tgattgagaa agttgttgta
     1921 gaattgcgcc ctcacctcct tgcaacctat gttcgggacc tcgcggatct gtttaactcc
     1981 ttctatcgtt tcgcgccggt tctgaaagct gaaggttctg ttcgtgacgc tcggctcaca
     2041 ctggttgatg ccagccggaa tacgctcgcc gcagcacttg atgtactggg tattgaagca
     2101 ctggagtcaa tgtagccaca ctgttgtgcc caacaggacg aaaatattct ttcagtctct
     2161 tccttttttc cgtagaaata ctttttcctt gggatacatt tcccgatttg cattggagaa
     2221 tatcgctgcg tgcctgattt tctgttacct cagataccgc gcagtatttg gtctctgcca
     2281 aaaattcaaa atattatata caaaatttaa atataaatta tatttgatta acaactggct
     2341 gacaagacac caaataacgg cgtatatgca tcagaatcaa tagttgggtt ataaatatgc
     2401 ctccctccaa tttccaatta acacagctct acccgtcaaa atcgggaggg atgtgaggtg
     2461 tgtattcatg gtaacattta gcccggcgca ggtttgcgtc aaagttggat cggcaggtaa
     2521 aaccaagtgc tgtctgccgg caggcaacat gtttgtccgt gccttcctcg caggtgccta
     2581 catcgcaatg ggtgcggcac tcgcaacggt tggctcgacc ggtgttgcag atgtactcgg
     2641 tgcaggtatt gctcagttca ttcttggagc actgttcccg attggtttag ttctcgtggt
     2701 tctcactggt gcagagctgt tcaccggtga tgcaatgttc gccccaatgg cagtccttca
     2761 gggccacact ggtcttaaag gcctcatata tctgtggatt gttgtgtata ttggtaacct
     2821 cattggttct ctcttcatgg cattccttgt cagctacggt ccatacactg catgggatgc
     2881 agcaggtgtt gcaacggcaa ccgcatttgg aacccgtgcc attgcaatcg caacggcaaa
     2941 ggtatcctac ttcggcccga tgggtatcct ctcggtcttc ttcaagggta ttctttgtaa
     3001 ctggcttgtg tgccttgctc tgttcctcgg tcttgcagcc gatgaagttg tttccaagat
     3061 ccttgcaatc tggttcccaa tcatggcttt cgttgccacc ggatttgaac actgtgttgc
     3121 aaacatgtac ttcatcccgg caggtatcat cacaaacatg atcgcaaatg gtggtgctga
     3181 cacggcggtt ctgaactggg gaaccatgtg gacaaacaac atcatctggt ccacccttgg
     3241 aaacatcgtt ggtgcagtca tcttcatggc agtaatctac tactactgct acaagagtga
     3301 gatctgtgca ctctgcgaaa ccaagtaatt gggcagacca aggttgaaaa gcgattaaat
     3361 cctacgcttt ctcaatctac ctctttttgt tttcttttac caacatcaaa gcatacaagt
     3421 atttatgggg gcggaatgca tttacagtaa gatagttccg gcatcccggg ggtttgtgta
     3481 actatatgaa aattcatggc gtaacaatcg gcatctctcc actgcatgtc cttgtagtgg
     3541 ttttctttct ggtactgttt agtgcatcat actttgccac atc
//
LOCUS       DALUAJ010000067         3342 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_11971,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000067 DALUAJ010000000
VERSION     DALUAJ010000067.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11971"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="O0X37_01535"
     CDS             complement(<1..673)
                     /locus_tag="O0X37_01535"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HJK61643.1"
                     /translation="MVPPETQILTVVLWLIVMKFCQMTTYPYLKPAVGNLSYGLAYPF
                     SILVLTFVTWYLGLVGLPVQLALLVFVTSGAYAFVSKKYDKTEILKNLKWDLVFFGAF
                     ALMLISRFLTPGIIPSGEKFMDAAFLGSIMLSPTVTPLDPWYAGGELSIYYYLGHWMC
                     GVLGILSNGTPTVVFNLMLPTVFALAAVSAYAIGVLLLKRHQRISMLVFILPNAALIW
                     HTIVGG"
     gene            complement(660..1640)
                     /locus_tag="O0X37_01540"
     CDS             complement(660..1640)
                     /locus_tag="O0X37_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HJK61644.1"
                     /translation="MWKKISAVLIPSLVAAALIVFMLARVWDDLLIAVQHLVPAYLIA
                     AILICLAAWYLRGWRYQYILKRLGTQIGLNFSTACIYVSQTANLIIPARLGDFVRLFI
                     LKHEKGTPYTNSFTSVVAERVYDVIIIAVLGLFALPMLISLVPAWFVWMIVFVLGAGA
                     VFFLFLLLSRRLHAENKIFKKILEVLDQLRQVSSTPASLAALSLSSAIIWMMDVTICY
                     LVSMMFGVTIPFMLVLLAIVIGNLVKAVPITPGGIGTYELALVITFEIGGVPAATATL
                     IAVVDHLIKNLVTLAGGVGSLYYFGDWAVSLLKRLFHEDAKKLKEEHGSP"
     gene            complement(1624..2340)
                     /locus_tag="O0X37_01545"
     CDS             complement(1624..2340)
                     /locus_tag="O0X37_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HJK61645.1"
                     /translation="MNPVHITVVLPVYNDVEALRSAIPKSLEALTEFGKTFELIVAED
                     GSSDGSRELVEEWERKDPRVRLLHSDERQGRGRALNRALAESNGEIFCYYDVDLATDI
                     KHLPELLTRIEDGAAVATGSRLMPESKIIRSGDREFASRSYNTLVRLFLGSCLRDHQC
                     GFKAYRADVLRELVPKIRAPHWFWDTESVVLAQRAGLRVDEFPVVWTQGPGTTVRFKD
                     VYGMGMDILKMWWRLHVEKN"
     gene            complement(2337..3329)
                     /locus_tag="O0X37_01550"
     CDS             complement(2337..3329)
                     /locus_tag="O0X37_01550"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJK61646.1"
                     /translation="MNGKLHPLTTPMEIFHYPCLSFFQKVRLGMFVLSSRRIDMAALD
                     NITAKEYLHEKVGEDIYDAFFAPLLASKFGPMKDDVSAAWLMSRIAIRSDRGAEGERL
                     GYLKGGWHRLVDSMVDRLEASGVDLRLSTPVNVLRISEGKWIINGEEFDAVVSTLPPQ
                     ITTSLMDKETLEMFSLPQLPYQGAACMTLGLAKDPAAGIYWTNMGDPAPYGAVVVHTN
                     FVPYDWYGEHVVYLASYFKDDPATDLKERMIDDFCNRFGIGKEIIHHADLYIDKFAGP
                     VYVTGYRNMIPNTDAGHNLFIAGMYSVENYPERSMDGSVRAGHRVAALLKEKLS"
BASE COUNT          922 a          876 c          758 g          786 t
ORIGIN      
        1 ctccaccaac aatagtatgc cagattagag cagcgttggg gaggatgaag acaagcattg
       61 atatccgctg atgacgcttt aacaggagaa cacctattgc atatgctgac acagccgcaa
      121 gcgcaaacac ggtcggcagc atcagattga acacaactgt aggtgtaccg ttggaaagaa
      181 taccaagcac tccgcacatc caatggccga ggtaataata gatggacagt tctccgcctg
      241 cataccaggg atccagcggc gtaactgttg ggctcagcat aatgcttccc agaaatgcag
      301 cgtccataaa cttctctccg cttggaataa ttcctggtgt caggaaccgt gagatcaaca
      361 tcagtgcaaa tgccccgaag aacaccagat cccatttcag gttcttaagt atctctgttt
      421 tatcatactt ttttgagaca aaggcatacg ctcctgaagt tacaaatact aacagggcaa
      481 gctgtaccgg tagaccaacc agaccaagat accaagtgac aaaggtcagt acaagaattg
      541 aaaacgggta ggcaagacca tacgaaaggt tacccactgc aggttttaga tacggatagg
      601 tggtcatctg gcagaacttc atcacaatca gccagagaac gaccgtcagt atctgtgttt
      661 cagggggaac catgctcctc cttcagtttt tttgcatcct catggaacaa ccgttttaac
      721 agtgacactg cccagtcgcc gaagtagtac agggagccca ctcctccggc aagggtaaca
      781 aggtttttga tcagatgatc caccactgcg atcagagtag ccgttgccgc aggaactcct
      841 ccgatctcaa acgtaatcac gagtgcaagt tcatacgttc cgataccacc gggagtgatc
      901 ggtaccgctt tcacaagatt tccgatcaca attgcaagca gtaccagcat aaacgggatc
      961 gtcactccaa acatcatcga gacaagataa cagatggtca catccatcat ccagatgata
     1021 gcagatgaaa gtgataacgc tgcaagtgat gctggcgttg aagacacctg tctgagctga
     1081 tctaacacct ccaaaatctt tttgaagatt ttattttccg catggagtct gcgtgaaagt
     1141 agaagaaaaa ggaaaaatac tgcacccgct cccagaacga aaacgatcat ccagacaaac
     1201 cacgcgggaa cgaggctaat cagcattggc aatgcaaata gtccaagaac tgcgatgatg
     1261 atgacatcat acacacgctc tgcgacaact gaggtgaaac tgtttgtata aggcgttcct
     1321 ttctcgtgtt tcagaataaa gagccgaaca aagtcaccga gccgtgctgg aatgatcaga
     1381 ttcgctgttt gggatacata aatgcaggcg gtagaaaagt taagacctat ttgcgtacct
     1441 aacctcttta ggatatactg gtaccgccag ccacgcaggt accatgcggc aagacagata
     1501 agaatcgctg cgataaggta ggcgggaacc aaatgttgga ctgcgatcag cagatcatcc
     1561 cagactcttg cgagcataaa cacaatcagt gctgcagcaa caagactggg gataagtaca
     1621 gcactaattt ttttccacat gcagccgcca ccacattttt agaatatcca ttcccatccc
     1681 gtacacgtcc ttgaaccgga ctgttgttcc cggtccctgt gtccaaacaa ccggaaattc
     1741 atccacgcgg agtcctgcac gctgtgcaag taccacagac tcggtatccc agaaccagtg
     1801 aggcgcacgg atctttggaa ccagctcccg cagcacatct gcccggtatg ccttgaatcc
     1861 gcactgatga tcccggaggc agcttccaag aaacagccgg accagtgtat tgtaactgcg
     1921 gctcgcaaac tcccggtcac cactccggat aattttactc tccggcatca gacgcgatcc
     1981 ggtagcaact gctgcaccat cttcgattcg ggtcaggagt tcaggaagat gtttgatgtc
     2041 ggttgcaagg tccacgtcat agtaacagaa tatttcgcca ttagactccg caagtgcacg
     2101 gttcagtgct cgtccccgtc cctgccgttc gtcgctgtgc agcagacgga cacgcggatc
     2161 tttccgttcc cactcctcaa ccaactcgcg gctcccgtcg gaactcccgt cctcggcgac
     2221 aatcagttca aaagtttttc cgaactcagt caacgcttca agagattttg ggattgcaga
     2281 tcgtaatgcc tcaacatcat tatagaccgg cagcactacg gtaatgtgta cgggattcac
     2341 gaaagtttct ccttgagcag ggcagctacg cggtgtcctg cacgaacaga accatccatg
     2401 ctccgttcgg gatagttctc cactgagtac attccagcaa tgaacagatt gtgtccggca
     2461 tcggtattgg gtatcatgtt ccgataaccg gttacatata cgggccccgc gaacttgtca
     2521 atgtacaggt cggcgtgatg aattatctct tttcctattc caaacctgtt acagaaatca
     2581 tcaatcattc tctctttgag atcggttgcg gggtcgtctt tgaagtatga ggcgagatac
     2641 actacatgtt cgccgtacca gtcataaggg acaaagttcg tgtgaacaac cactgcccca
     2701 tacggtgcag gatctcccat attggtccaa taaatccctg ctgccggatc cttggcaagt
     2761 cccagcgtca tacaggctgc tccttggtat ggtaactgag gcaaggagaa catttctaac
     2821 gtttctttgt ccatcaaaga cgttgttatc tgtggcggaa gagtggaaac aactgcatca
     2881 aactcctctc cgttgatgat ccattttcct tccgaaatcc ggagtacatt taccggcgta
     2941 gaaagccgca agtctacccc tgatgcttcc aggcgatcca ccatagaatc aacaagcctg
     3001 tgccacccgc ctttcaggta tccgagccgt tcaccttccg caccgcgatc ggatctgatt
     3061 gcaatccggc tcatcagcca cgccgcagac acatcatctt tcatcggacc aaacttactt
     3121 gcaagaaggg gagcaaaaaa tgcatcatag atatcttcac caactttttc atggagatac
     3181 tcctttgcag tgatgttgtc aagtgccgcc atatctattc tgcgggaact taggacgaac
     3241 attccgagtc gtaccttttg aaaaaaactc aggcaaggat aatggaaaat ttccatgggt
     3301 gtggtaagcg gatgcagttt tccgttcata taatacccgg tc
//
LOCUS       DALUAJ010000068         5592 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_12102,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000068 DALUAJ010000000
VERSION     DALUAJ010000068.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12102"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /gene="gatD"
                     /locus_tag="O0X37_01555"
     CDS             <1..770
                     /gene="gatD"
                     /locus_tag="O0X37_01555"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HJK61647.1"
                     /translation="TDTMLYSAAAVSFMLEIPAPVIFVGAQRSADRPSSDNAMNAICS
                     AKAGVSDLGEVVVCMHATSDDVNCALHRATRVRKNHTSRRDAFQSIGRVPVGMVSYPD
                     GNVTLGKDAVLRGTHELRLFDRLEPHCGLLQYYPGMNPAVFDAFSGYAGLIIAGTGLG
                     HVGTDCIEKIAALTAAGTFVVMTSQCQAGEVCDRVYETGRDLLNAGVVEGGNMLPEVA
                     LVKLMWVLGNTTRREDAVRMMQTNLKGELDYDLWREM"
     gene            771..2609
                     /gene="gatE"
                     /locus_tag="O0X37_01560"
     CDS             771..2609
                     /gene="gatE"
                     /locus_tag="O0X37_01560"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HJK61648.1"
                     /translation="MTDFDYEALGLKAGIEIHQQLNTKEKLFSHTPTIIRDSSEHTGE
                     VKRYLRVATSEMGDVDRAAKEEMLAARLFTYYTYDTTGLVEIDEQPPAPLNPDALDLV
                     LTIAKMFNMTPLPQIHTMRKMIVDGSATSGFQRTALVALNGAIDQTCRIETVAVEEDA
                     CQRVTDQVFSVDRLGIPLIEITTAPCMKTPEQVHDVAQYIGMTLRSTGRVKRGLGTIR
                     QDVNVSIRDGARVEIKGVQELDLIAEVVRREVQRQLSLLAIKDELIARNALVNGEVYD
                     VTSVFAKTQSQVLKKAKVILGTVLHGFAGLVGMEIQPGRRLGSEMSDYAKKCGVGGLF
                     HTDELPAYGVTAEEVSVLKESLGAGEQDAVIIVAATEQKSRCAIQMIIRRAKMAMEGV
                     PEETRKMLEGGSTAYMRPLPGAARMYPETDILPVPVTQEYWESITTPELLIDKAERFV
                     HEYGLDAGMAHQIAFSEKLPLFERAVAEGIRPVFAARTILASLKELSRAGISPDVIPD
                     DSVIAVMKAVETGHAAKEAVSEILSACASGMTLEEAIETVAPAFSREELQALVHTIVS
                     ERVDFIKSRGKAALGPVMGVVMKEVRGRIDGKVVSEVLEEELGRII"
     gene            2708..2977
                     /locus_tag="O0X37_01565"
     CDS             2708..2977
                     /locus_tag="O0X37_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5350 domain-containing protein"
                     /protein_id="HJK61649.1"
                     /translation="MGKTGSINWHQIKGVNGQIRLVPQKEGEVKKPGPNQRFKRASIV
                     TKIESRMANAPQQGRRGPKIADPRVRRRIRRSKKSMMGAKAKSKR"
     gene            3218..4456
                     /gene="mmp10"
                     /locus_tag="O0X37_01570"
     CDS             3218..4456
                     /gene="mmp10"
                     /locus_tag="O0X37_01570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833686.1"
                     /note="Mmp10 (methanogenesis marker protein 10) is a
                     cobalamin-requiring radical SAM methyltransferase that
                     creates the methylarginine modification to methyl coenzyme
                     M reductase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl coenzyme M reductase-arginine
                     methyltransferase Mmp10"
                     /protein_id="HJK61650.1"
                     /translation="MVHLTVDIGGRPGIDCRGFCAYCYFKHAKDVPPFGCRYCLPFLK
                     GCDYCSRGVKEEYSGFLPLRTVAESFLADLQMVTEDVTRITISGGGDPSCYPDFRDLV
                     ELLGTLDIPLHIGYTSGKGFDDPEIADFLVENHLTEVSFTVFAADAELRKKWMHDPTP
                     EASLEIIRRLAKKIDVYAAVVILPDVNDGDVLTKTLSWLSDIGVKGVILMRFANRPEQ
                     GLILDNAPILPGQRVHTVEEFADLVQASAAAFPHLRISGTPLYDPLFDSPFAIRKMPE
                     LLDHLPKINKKATIITGSVAAPYIKEIIRSRGGDPDSVIAVNKEIACLITIEDLTSLN
                     LQDLSDTVIIPGRAFVHDAEAEKVLSRDGVYRTVIRGPEMLSADGETSMGMTEVDVLT
                     MEMEGFSTLIRLINQYGSTT"
     gene            4453..4923
                     /locus_tag="O0X37_01575"
     CDS             4453..4923
                     /locus_tag="O0X37_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HJK61651.1"
                     /translation="MNYAELYEKIGYTFKNEAYLNQAMTRTAYARENETPMSETMDAL
                     AVLGDAVLDLVIICEIMADGEYDKGEITRKKIDAVNMTVVRRIAEQLDLPSYMRWGKG
                     ELRMQIWTSGRVSAECFEALIGAAYMDGGVEAATTIIRTVRTPQGQTPNSINEP"
     gene            5090..5371
                     /locus_tag="O0X37_01580"
     CDS             5090..5371
                     /locus_tag="O0X37_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HJK61652.1"
                     /translation="MVRVKASHILVKTEAEAKEIIRKLSSGDDFAKLAKLYSQCPSGR
                     AGGDLGYFGKGQMVKPFEDAAFKAKVGEVVGPVKTQFGWHVIKVTDMKN"
     gene            5390..>5592
                     /locus_tag="O0X37_01585"
     CDS             5390..>5592
                     /locus_tag="O0X37_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61653.1"
                     /translation="MDEKTFLCCIEAIKSSDVAIQEDAANTIAALTEERWLPQILYHL
                     NDANPLVRRVMLWTLRNYTGKIS"
BASE COUNT         1530 a         1254 c         1521 g         1287 t
ORIGIN      
        1 gaactgacac tatgctctac agtgcagctg cagtttcatt catgctggag attcccgccc
       61 cagtaatctt cgttggtgca cagcggtctg cggatcgtcc aagcagtgac aatgcaatga
      121 atgcgatctg ctcggcaaaa gcaggtgttt cagaccttgg cgaggttgtt gtctgcatgc
      181 acgcaacctc ggatgatgtg aattgtgccc tgcaccgggc aaccagggta cgcaagaatc
      241 acacatcgcg cagggatgca ttccaaagca ttggaagagt accggttgga atggtttcat
      301 acccggatgg taacgtaaca cttggaaaag atgcagttct gcgcggaacg catgagctcc
      361 ggttgttcga tcggcttgaa ccgcactgtg gtctccttca gtattaccct ggaatgaatc
      421 ccgcagtgtt tgatgcattc agcggttatg caggactcat tatcgcggga actggtcttg
      481 gtcatgtggg gactgactgc atcgaaaaaa ttgctgcact aactgctgca ggaacatttg
      541 tggtgatgac ctcgcaatgc caagcaggag aggtctgtga tcgtgtgtac gaaacaggac
      601 gagacctgct taatgcaggt gttgtagagg gagggaacat gctgccggaa gtggcactcg
      661 tgaagctgat gtgggttctt ggtaacacca cccgacgcga ggatgctgtg cgaatgatgc
      721 agacaaacct gaaaggcgaa cttgactatg atctctggag ggagatgtaa atgaccgatt
      781 tcgactatga ggccctggga ttaaaggcag gaatcgagat ccaccagcag ctcaatacaa
      841 aggaaaagct gttctcccac accccaacaa tcatccgaga ttcatccgag cacaccggcg
      901 aggtgaaacg gtacctgcgt gtggcaacat ctgagatggg agacgttgat cgcgcagcaa
      961 aagaggagat gcttgcagcc aggctgttta cgtactacac ctatgacaca acaggtcttg
     1021 tggagattga cgaacagccg cctgcaccac tgaatcccga tgcattggat ctggtgctga
     1081 cgattgcaaa gatgttcaac atgacaccac tgccgcagat tcacacgatg cggaagatga
     1141 tcgtggacgg atctgcgaca agcggatttc agcggactgc actggttgca ttgaatggtg
     1201 caattgatca gacctgcagg attgaaacgg tcgccgttga agaagacgcc tgccagcgcg
     1261 ttaccgatca ggtgttttca gttgaccgcc ttggcatccc gttgatcgaa attacaaccg
     1321 cgccatgtat gaaaacaccc gagcaggtac atgatgttgc ccagtacatc ggtatgacat
     1381 tgcgatccac ggggcgggta aagcgcggcc ttggtacaat tcgtcaggat gtgaatgtct
     1441 ccatccgcga tggagcacgc gtggagatta agggggtaca ggaactggac ctgattgcag
     1501 aagtggtccg tcgtgaagta cagcgccagc tttcactgct cgcaatcaaa gatgaactga
     1561 tagcgcgcaa tgcactggta aacggggagg tgtacgatgt aacatctgtc tttgccaaaa
     1621 cacagtcgca agtactgaag aaagcaaagg tgattctcgg aacagttctc cacgggtttg
     1681 cagggcttgt tggaatggag atccagcctg gccggagact tggctcggag atgtcggatt
     1741 atgcaaagaa atgcggagtc ggcggcctgt tccacaccga cgagctgcct gcatacggcg
     1801 tgaccgctga agaagtatca gttctgaaag aaagtcttgg tgctggtgag caggatgcag
     1861 ttatcatcgt ggcagcaaca gagcagaaat ccagatgcgc gatacagatg attatccgcc
     1921 gcgcgaagat ggcaatggaa ggtgttccgg aagagacacg caagatgctt gagggggggt
     1981 caaccgctta catgaggccg ctgccaggtg cagcacgaat gtatccggaa acggatattc
     2041 tgccggtacc ggtcactcag gagtactggg aaagtatcac aacgccggaa cttttgatcg
     2101 acaaagctga gcggtttgtc catgagtacg gtcttgatgc ggggatggca catcagatag
     2161 cattttccga gaaactgcca ctgtttgaac gggcagttgc cgagggaatt cgtcctgtgt
     2221 ttgcggcgcg caccatactt gcttccctaa aagaactgtc gcgtgcggga atctcaccag
     2281 acgtaatccc ggatgactct gtcatcgcag taatgaaggc agttgagacg gggcatgcag
     2341 caaaagaggc agtgtcagag atattatcgg catgcgcaag cggtatgacc ctggaagaag
     2401 caattgagac ggttgcacct gcattttccc gcgaagaact gcaggccctc gttcacacaa
     2461 ttgtttccga acgtgttgat ttcataaaat cacgcggaaa ggcagcactc ggacctgtga
     2521 tgggagtagt gatgaaagag gtgcgtggca ggattgatgg taaggtggtc tcggaggttt
     2581 tggaggagga acttggacgg atcatctgat ccgctccatc agatctggtt tcaaggagct
     2641 tacaccactc cgcagtcatt gagatatgtt taagtgaata gggcacgaac aaggtaagga
     2701 gttcaatatg ggaaagacag gtagcatcaa ctggcaccag atcaaaggtg ttaacggtca
     2761 gatcagactc gttccccaga aagagggaga ggtcaagaaa ccaggaccga accagagatt
     2821 caagagagca agcattgtta cgaagatcga aagccgtatg gcaaatgccc cacagcaggg
     2881 ccgccgtgga ccaaagatcg cagacccgcg cgttcgccgc cggatccgga gatcaaagaa
     2941 gtccatgatg ggcgcaaaag caaagtcaaa gcgctgatct tttcggacac aatacttcca
     3001 tccccaattt atgtattccc acgggagtac atttccaatt ttttctgttt gctgaaactc
     3061 atctgtttat ttcgtggtag tagactcagt ctacaaagat cctaaatgaa aaactctcta
     3121 gcatgatgtc atgaaaaagt aggatttgct agacgaggga acgccattta tctcaaccat
     3181 tcataacccc gcagcactaa tggatataga agagactatg gtgcatctta ccgttgatat
     3241 aggcggacgt cccggcattg actgccgggg tttttgtgcc tactgctact ttaagcatgc
     3301 aaaggatgta cctccgttcg gctgcaggta ctgtcttccg tttttgaaag gatgtgacta
     3361 ctgctcccgc ggtgtcaaag aggagtactc aggatttctg cctttgagga ccgttgctga
     3421 atcatttctt gcagaccttc agatggtcac agaagatgtc acccgcatca ccatctcagg
     3481 cggtggtgac cccagttgtt accccgattt tcgtgatctg gtagagcttc ttgggacact
     3541 ggatataccg ctgcacatcg gctacaccag cggcaaagga tttgatgacc cagaaatagc
     3601 agactttttg gttgaaaatc accttacaga ggtttccttc accgtatttg ccgcggatgc
     3661 agaactcaga aaaaaatgga tgcatgatcc aacgcctgag gcatcacttg agatcatccg
     3721 gcgtcttgca aagaagatcg acgtgtatgc agcggttgtt atcctgccgg atgtaaacga
     3781 cggtgatgtg ttgaccaaaa cactctcatg gctctctgat atcggggtaa agggagtcat
     3841 tctgatgcgg tttgcgaaca ggcccgaaca gggactgatc cttgacaatg ccccaattct
     3901 cccaggtcag cgcgtacaca cagttgagga atttgcagat cttgtacagg cgagtgctgc
     3961 cgcatttccc catctcagga tctccgggac tccattgtat gaccctctgt ttgattcccc
     4021 attcgccatc cgaaaaatgc cagagttgct ggatcatctc ccaaaaatta ataaaaaagc
     4081 tacaattata acaggatctg ttgcagcccc atatattaag gaaataatca gatcccgcgg
     4141 cggagaccca gactcggtca ttgcagtgaa caaagagatt gcatgtctga taaccattga
     4201 ggatcttact tcactgaacc tgcaggatct gtctgatacc gtgattattc ccggccgtgc
     4261 attcgtccat gatgcagagg cagaaaaagt actgtcgcgt gatggggtgt atcgtacagt
     4321 catccggggc ccagagatgc tttcagccga cggggagaca agtatgggca tgacagaggt
     4381 tgatgttctc accatggaaa tggaaggatt ttctactctc atcaggctga taaatcagta
     4441 tgggagtact acatgaacta tgccgaattg tacgagaaga taggatacac attcaaaaat
     4501 gaagcgtatc tcaaccaggc aatgacacga accgcgtacg cccgtgagaa cgaaacaccg
     4561 atgagtgaaa ccatggatgc acttgcagta ctcggggatg cggtactgga tctggtaatt
     4621 atctgtgaga taatggcaga tggagagtat gacaaggggg agattacgcg taaaaagatt
     4681 gatgctgtga atatgacggt cgtccggaga attgccgaac aactcgatct tccatcttac
     4741 atgcgatggg gaaaaggtga gctccgcatg cagatctgga cgagcggacg tgtttcagca
     4801 gagtgctttg aagcactgat tggtgccgca tacatggatg gaggtgttga ggcagcaacg
     4861 acaatcatca gaaccgttcg tacaccgcaa ggacagaccc ctaattccat caatgaaccg
     4921 tgaacctcga gaacagaaca taccagatag attgcaatat cctccatatc accagttctt
     4981 ttttttgata atttagacaa tacaaatttg catgagatag agaatacata gtgtaacata
     5041 actagcgtgc tatcatagtt tattgcgttg cagtgcgata tgctatggta tggtacgggt
     5101 aaaggcatcc catattttgg taaagacaga ggcagaggca aaagagatta taaggaagct
     5161 ttccagcggg gatgattttg caaagcttgc aaagctctac tcccaatgtc catctggaag
     5221 agcaggtggg gatctgggat acttcggtaa aggacaaatg gtaaagccct ttgaggacgc
     5281 ggcatttaaa gctaaagttg gtgaggttgt agggcccgtc aaaacgcagt ttgggtggca
     5341 tgtgatcaaa gtaactgata tgaaaaacta gatatacagt cagaaggcaa tggacgaaaa
     5401 gacctttttg tgctgcattg aagcaataaa aagcagcgat gtggcgatac aggaggatgc
     5461 ggcaaatacc atagcagcgt taacggaaga gcgctggctg ccgcaaattt tgtatcatct
     5521 caatgatgca aaccctctcg tacgcagggt gatgctctgg acgctgcgta actacaccgg
     5581 aaagatatcg ta
//
LOCUS       DALUAJ010000069         1680 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_12104,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000069 DALUAJ010000000
VERSION     DALUAJ010000069.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12104"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="O0X37_01590"
     CDS             complement(<1..230)
                     /locus_tag="O0X37_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61654.1"
                     /translation="MGERSVQLINYTPHTIGICQPDGTIISIPSSGEIRVMPVKHNNG
                     KLPNGVQLQTVTYQKLILPDRQPGTWLIVSGYV"
     gene            complement(385..1230)
                     /locus_tag="O0X37_01595"
     CDS             complement(385..1230)
                     /locus_tag="O0X37_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HJK61655.1"
                     /translation="MIMIGDCRQMLPTIEPESIDCIVTSPPYYNLRDYGMPGQIGAEN
                     TVEEYIDNLTDVFSKSRTVLKPNGALWLNVADTYAQKNLLGIPWQLAFALRNDGWILR
                     QDIIWCKPNPMPESVVDRCTKAHEYLFLLTKSAKYYFSLDSVKEEAQYKPSDSPKVKK
                     SCRYGGKKYALNQDMFYRTKSGNIYNYTGTRHPRSVWTIPTQPFSGEHFATFPEELAR
                     RCILAGCPPSGIVMDPFCGSGTTGKVAYDLGREFCGIELNPKYAEIATKRIGISRQMP
                     LEMIV"
     gene            complement(1227..1388)
                     /locus_tag="O0X37_01600"
     CDS             complement(1227..1388)
                     /locus_tag="O0X37_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61656.1"
                     /translation="MTDEIRGIDGCIAAHCHQLRTREGQHYCKDGMCGGKALDGRRFW
                     CGVHRRVDT"
     gene            complement(1385..>1680)
                     /locus_tag="O0X37_01605"
     CDS             complement(1385..>1680)
                     /locus_tag="O0X37_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61657.1"
                     /translation="SGKVSSMDTNLIRSGNLYVCSQCGCEFFLPSPPERTGPPSTIPP
                     YLYCPHCSKEAGIRVDSTDERELIHTHLQTKTAYLALLDQRTRELREMEVVQQ"
BASE COUNT          340 a          408 c          456 g          476 t
ORIGIN      
        1 acgtatccac tgacgatcag ccaggtgcca ggctgccggt ccgggaggat cagtttctgg
       61 tatgtgacgg tttgcagctg gacgccgttg ggaagttttc cgttgttgtg tttgacaggc
      121 attacgcgga tctcgccgga ggaggggatc gagatgatcg tcccgtccgg ctgacagatg
      181 ccgatggtgt gtggtgtgta gttgatgagt tgcacgctcc tttcccccat ttttcgctag
      241 cattcgctat tcatgagtgt gtgttcagca ttcgtcgatg gtgatagcca ggttgatgac
      301 ctggcctttc aggttctgga atacctggtc ttcagtgaga cttaagcagg agactgatat
      361 cttccagcgg tgagatcgct tggatcatac tatcatctcc aagggcattt gtcgagatat
      421 tccgattcgt ttcgttgcaa tttctgcgta cttaggattc agctcgatcc cgcagaactc
      481 acgtccgagg tcatatgcta ctttcccagt cgttccgcta ccgcagaacg gatccataac
      541 aataccactc ggcgggcaac ctgcaaggat acaacggcgg gcaagttctt ccggaaatgt
      601 tgcgaagtgc tctcctgaga agggctgtgt tgggatagtc cacaccgatc tcggatgtct
      661 ggttccggtg tagttgtata tgttcccaga ctttgtgcga tagaacatat cctgattcaa
      721 agcgtatttc ttacccccat atctgcagga ttttttgacc tttggcgaat cactcggttt
      781 atactgcgct tcttccttga cactgtcaag agaaaagtag tatttcgcgg atttggtgag
      841 caagaacaga tattcgtgtg ctttggtgca tctatccacg acgctttctg gcatggggtt
      901 cggtttgcac cagatgatgt cctgtcgaag tatccaccca tcattgcgga gagcaaatgc
      961 cagttgccac gggatgccaa ggaggttttt ttgtgcgtag gtgtcagcta cgttgagcca
     1021 gagcgcccca tttggtttga gaacagttcg tgacttagag aatacatctg tgagattgtc
     1081 aatgtattct tcaactgtgt tttcggctcc aatttgccca ggcatgccat agtctctcaa
     1141 gttgtaatat ggcggactgg ttacgatgca gtcaatactc tctggctcga tggtagggag
     1201 catctggcga cagtcaccta tcattatcat gtgtcaaccc tccggtgtac tccacaccag
     1261 aaccgccttc cgtcaagtgc ttttcctccg cacatgccgt ccttgcagta gtgctggccc
     1321 tctctggttc ggagctggtg gcagtgtgcc gcgatacagc catcaatccc acgtatctcg
     1381 tcggtcattg ctggaccacc tccatctccc ggagttctct tgtgcgttgg tccagcagtg
     1441 cgagataggc tgttttagtc tggagatgtg tgtggatcag ttccctttca tcagtgctgt
     1501 caacacgaat tcctgcttct ttgctgcaat gcgggcagta aagataggga gggattgtgc
     1561 tgggaggacc ggtccgttcc ggtggtgagg gcaggaagaa ttcacagccg cactggctac
     1621 agacatagag gttgccggac cggatgagat tagtgtccat gctgctcacc tttccggaca
//
LOCUS       DALUAJ010000070         2122 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_12646,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000070 DALUAJ010000000
VERSION     DALUAJ010000070.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12646"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="O0X37_01610"
     CDS             <1..418
                     /locus_tag="O0X37_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HJK61658.1"
                     /translation="LNGELLPANGEVIDGCGYMHGHTIPAPELAGKLILCGHHHPVVN
                     IYDEVGCSLRGTPGYLLAEIDSTALELPETQTTTRVLLVPAFYELAGGLDVRLIPGNK
                     ISPIAKAIRTETAEVFLKDGTYVDTWENLSPNPEEK"
     gene            415..>2122
                     /locus_tag="O0X37_01615"
     CDS             415..>2122
                     /locus_tag="O0X37_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJK61659.1"
                     /translation="MTGAIEELRESLHPKIRDALDKRGFDEFSEIQMRAVPKLIHGDN
                     AVLIAPTGTGKTESALLPIFHRMLTEPHPPGFTTLYITPLRSLNRDMMNRLEWWGKEL
                     GLRIAVRHGDTTQNDRRKQATHPPNLLITTPESLQAMMMGKVLRKHLAGVRFIVIDEI
                     HELAGSKRGAQLSVGLERLMELSGEVQRIGISATVGNPDVIGKFLCGKRPYTVVQVPV
                     AKTLDITVRFAGESFADQTKLIEKCIDSHTSTLVFTNTRSVAEAIGHALRERGDIDVH
                     HGSLSREMRIDAEDRFRAGLTRGMISTSSMELGIDIGQIDHVVQFNSPREVARLMQRT
                     GRAGHQLHATSKGTVLATGFDDCIESMVIVRKAMENEPENVRPHINAADVIANQIAAL
                     AVERGEIAIKKIEEIFSRSYCFEDAGPLIREVIAQMEKHYLIRTENDTVITKARARRY
                     LSQNLSMIADEKKSIVFDVVSRKPVGTLDESFVISWISSGAVFVARGQMWRVLDIDED
                     RIMVEPAKNAKGELPSWEGEQIPVPFTVATEAGRLRRLRTFEGYHADERTVQFAEKIL
                     AEM"
BASE COUNT          550 a          564 c          561 g          447 t
ORIGIN      
        1 cctgaatggg gaacttcttc cggcaaacgg cgaggtcatt gatggatgcg gttacatgca
       61 cggccacacc attcctgcac cggagcttgc cggaaaactc atcctctgcg gtcatcacca
      121 tccggttgta aacatctacg acgaagtcgg atgttcactc cgcggaaccc ccggatatct
      181 tctggcagag atagacagta ctgcccttga acttccggag acacagacca cgacccgcgt
      241 ccttctggtt cccgcatttt atgaacttgc aggcggactc gatgtccggt taattccggg
      301 gaacaagatc tcaccgattg caaaagcaat cagaaccgag accgcggaag tgttcctaaa
      361 agacgggacc tatgttgaca cctgggaaaa tctcagcccg aacccggagg agaaatgacc
      421 ggagctattg aggagttgcg ggaaagtctg catccaaaaa ttcgggatgc cctcgataaa
      481 cggggttttg acgagttctc tgagattcag atgcgggcag tccctaagct catccacgga
      541 gacaatgctg ttctgattgc accgaccgga accggaaaga ccgaaagtgc ccttctgccg
      601 atatttcacc ggatgcttac cgagccgcat ccgccaggat ttaccacact ctacattaca
      661 ccgcttcgat ctctcaaccg tgacatgatg aatcgtcttg agtggtgggg aaaagaactc
      721 ggactgcgta ttgcggtccg ccacggcgac accacacaaa atgatcgccg caaacaggca
      781 acacacccgc cgaacctgct tatcacgacg ccggaaagtc ttcaggcaat gatgatggga
      841 aaagtgctgc gcaaacatct tgccggtgtc cgtttcattg tgatcgatga gatccatgag
      901 ctcgcgggat ccaaacgcgg cgcacaactc tctgtcggac tggagcggct gatggaactc
      961 tctggagagg tacagcgaat cggcatctcc gcaacggttg gtaatcctga cgttatcggg
     1021 aagttcctct gcggcaaacg cccgtacacc gttgtgcagg taccggtcgc aaaaaccctg
     1081 gacataacgg tcaggtttgc tggcgagtca tttgcggatc agacaaaact gatcgaaaaa
     1141 tgtatcgact cccacacctc aacactcgtc ttcaccaata ctcgtagcgt agctgaggca
     1201 atcggccatg cactgcggga acgcggagac attgatgttc atcacggttc tctttctcgt
     1261 gagatgcgaa ttgatgccga ggaccgattc cgtgcagggc tcacgcgggg aatgatcagc
     1321 acatcctcca tggagctcgg catcgacatt ggccagatcg atcacgtagt acagttcaac
     1381 tcaccgcgtg aggttgccag acttatgcaa cgaaccggcc gtgcaggaca tcagctgcat
     1441 gcaacatcaa agggaactgt tcttgcaacc gggtttgatg actgcattga gtcgatggtg
     1501 atcgttcgca aagcaatgga gaacgaaccg gaaaatgttc ggccgcacat caacgcagcg
     1561 gacgttatcg caaaccagat cgcagcactt gcagttgagc gcggcgagat tgccatcaaa
     1621 aagatcgaag agatcttttc ccgcagttac tgttttgaag atgcaggccc gctgatccgt
     1681 gaggttattg cacagatgga gaaacattac ctcatcagaa ccgagaatga cacggtcatc
     1741 accaaagcac gcgcacgtcg ttacctctcg cagaatctgt cgatgattgc ggacgaaaag
     1801 aagagtatcg tctttgatgt tgtatcacgc aaacccgtcg gaaccctgga cgaatcattt
     1861 gtgataagct ggatctcatc cggtgcggtg tttgttgccc gcggccagat gtggcgagtt
     1921 cttgatattg acgaagaccg gatcatggtc gaacccgcaa agaatgcaaa aggagaactg
     1981 ccgtcatggg aaggagaaca gattccggtt cccttcacgg tcgcaacaga ggcaggacga
     2041 ctccgtcgcc tgcgtacctt tgaaggttac cacgcggacg aacgaacagt acagttcgca
     2101 gaaaaaattc ttgctgagat ga
//
LOCUS       DALUAJ010000071         1986 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_12648,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000071 DALUAJ010000000
VERSION     DALUAJ010000071.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12648"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="O0X37_01620"
     CDS             <1..959
                     /locus_tag="O0X37_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HJK61660.1"
                     /translation="IATLIGTPANGILVAQMQTIFPDAPAVDFFTWLKFGIPLLLIFI
                     PVTWAWLTHVAYRKMPKTLEHAKKALATEVAALGPMSRGEKNTLAVFLLTAFLWIFEK
                     NKDFGTFTIPGLEMIFPGIDDCTVAIFGALLLFLLPVNWKKQEFTMNWQWAVRIPWGI
                     LLLFGGGMCLSAAFIESGLAQAIVDYFTVLNGVPVVLLILIVALVVCFLTEVTSNTAI
                     ASVMMPILAVTGLSLGINPWILMMTAAVCSSFAFMLPVATPPNAIAYSTEYVEMKDMV
                     KAGWAMNLLGIAIFMILLYTVVLWAFGFTMDLPEWAFTTTIN"
     gene            1060..1710
                     /locus_tag="O0X37_01625"
     CDS             1060..1710
                     /locus_tag="O0X37_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61661.1"
                     /translation="MKYLAAGLAAILACCFVFSAGCINTTPSGPIAAGDNVTVEFTMY
                     IGDTVMVTSNETKYNQILTSGQMPVPLILTEDPIVTAGSTTDNDDLTIPTKTHSNPYW
                     FLIGEYNVIAGNIVGLEKGDTICIPIYAEGTEKTGFWTNEQCDEQGLNKQLLKAGDRL
                     TYTRTIGDATSTEDGSYDQNNLTNQQTMIHQLLVIDVTAEGVTYQELYDTVEITVV"
BASE COUNT          530 a          413 c          482 g          561 t
ORIGIN      
        1 gaattgcgac actgatcgga actcctgcaa acggaattct cgttgcacag atgcagacaa
       61 ttttcccgga cgcacctgcg gttgatttct ttacctggct gaagtttgga attccgctgc
      121 tgctgatttt catcccggtt acctgggcat ggttaacgca tgttgcatat cgtaagatgc
      181 caaagacgct tgaacacgca aagaaagcgc ttgctactga agttgcagct cttggcccga
      241 tgtcacgcgg cgaaaagaat acacttgcgg tctttctgct gacagcattt ttgtggattt
      301 tcgagaagaa caaagacttt ggaacattca cgattccggg acttgagatg atattccccg
      361 gcattgatga ttgtaccgtt gccatttttg gtgctcttct actgttcctg ctcccggtca
      421 actggaagaa acaggagttt accatgaact ggcagtgggc agtacggatc ccgtggggta
      481 ttctgcttct ctttggcgga ggtatgtgtc tgtctgctgc atttattgag agtggtcttg
      541 cccaggcaat tgtggattat ttcacggtgt taaacggtgt gccggttgtt ctcctgattc
      601 tgatagttgc gctggtggtc tgtttcctga ccgaggttac atcaaataca gctatcgcat
      661 cggtaatgat gccgattctt gcggtaaccg gtctgtcact tggcattaac ccatggattc
      721 tgatgatgac cgcagcggtc tgttcgagct ttgcatttat gctgccggtc gcaacaccgc
      781 caaatgcgat tgcttacagt acagagtatg ttgagatgaa ggacatggtg aaggcgggat
      841 gggcgatgaa ccttctgggt attgcaatat tcatgattct gttgtacacg gtggttctgt
      901 gggcatttgg gttcacgatg gatctgccgg agtgggcatt cacaaccaca ataaactaaa
      961 ctcttttttt actagaaacc ggcggacgaa gataaggaaa tatagacgac gagctttgtt
     1021 ccataaggtt gttaatgtat agtgtcctat acatcttgca tgaaatatct cgcagcagga
     1081 cttgctgcta ttttggcatg ttgttttgtg ttttccgcag gatgtatcaa tacaacacca
     1141 tctggaccaa tagcagcagg tgataatgtc accgttgagt ttaccatgta tatcggcgat
     1201 accgtaatgg tgacgtcgaa cgagacaaag tataatcaga ttttaacatc aggacagatg
     1261 ccagtcccac ttattttaac cgaagatccg attgttactg cgggttccac cactgataac
     1321 gacgatctta ctatccctac aaaaacccac tcaaaccctt actggttcct gattggtgag
     1381 tacaatgtaa ttgcaggaaa cattgttggt ctcgaaaaag gtgatacaat ctgtattcca
     1441 atctatgcgg agggaactga aaaaacgggg ttctggacaa atgagcagtg tgatgaacag
     1501 ggactcaaca aacaactgtt aaaagcaggg gaccgcctga cgtatacacg aacgatcgga
     1561 gatgcaacca gtacggagga cggaagctat gatcagaata acctcacaaa tcagcagacg
     1621 atgattcatc agcttctggt cattgatgta actgctgaag gagttaccta tcaagaactc
     1681 tatgatacag ttgaaattac agttgtctaa gtaatacagc agtgcgcggc aggaattctt
     1741 acttgccgca ggagatcttt ttttgttaaa tgatttttcc cataaattct ttgtggggtc
     1801 gggtataagg atttcagact cagtccactg agtttgatga aaaaatgtca ggattgagac
     1861 tccaacaact atcccaccca ttatcaattc ataggtctta ggagggacat atttaagaaa
     1921 caaaaagggc aactaccata acgtaatacg tgtgttgtca cgagtgtatc ggacagtgag
     1981 tattcc
//
LOCUS       DALUAJ010000072         4246 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_12695,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000072 DALUAJ010000000
VERSION     DALUAJ010000072.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12695"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            56..763
                     /locus_tag="O0X37_01630"
     CDS             56..763
                     /locus_tag="O0X37_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HJK61662.1"
                     /translation="MISVIVPTFNEEEGVEACLKSLCDQTLPRDQYEIIVVDGNSKDK
                     TREIAEKYADKVFIQTSKKVGGARNDGAMAAKYDILATTDADCFLPRDWLEKILADFA
                     KYPDASTIYGIVYPLEPGIKHKLSLLGYNIVSRIGYWTGTIYMTLGCNTAFRKDLFME
                     AGMYITADAGDDFEIARRMKKYGRVRLDTNLKVGFSMRRYIEFGAIKSIYQWLYIVAH
                     GGSSEKYQYAGKEYGKK"
     gene            complement(771..1424)
                     /locus_tag="O0X37_01635"
     CDS             complement(771..1424)
                     /locus_tag="O0X37_01635"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HJK61663.1"
                     /translation="MLTPTRRSILKRTLLLCIALGVMAVGVALSIKADLGTSPISSVP
                     YVVSLMSEMSVGTATILINAILLVGEILIMRRQFQPIQLLQLAVVTLFGWLIDLCLWG
                     LDGLTYADYGQQWMICIAGIIILGIGVSLEVTANLIPLPGEGLILSICTVLPIKFGNM
                     KVITDVSLVLVAVLLSFAFLSGLYGVREGTIAAAICVGLVAKTVSRLIGPYQRRLTS"
     gene            complement(1579..2724)
                     /locus_tag="O0X37_01640"
     CDS             complement(1579..2724)
                     /locus_tag="O0X37_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HJK61664.1"
                     /translation="MTVVGVACCGSYDRDAVLTAVEKAVNAAGGLPPVRGKRVLIKPN
                     LLSDLGADSSVTAHPEIVRAVCRMVLDAGGQPIIADSPGAGHLYTPKTLRRIYERSGM
                     AAVAEETGAELSFDTGSAEVPCPNGTVMKRFTVITPAQNADVIISVCKLKTHMFTKFS
                     GAVKNTFGVVPGLDKPVFHSRFVDQQDFSEMLVDLNELIGVDFVIMDAVVGMEGNGPM
                     GGDPKFCGYVLASNSVYGLDYSAQRLIGMDPDQIATTAAARRRGLLDTVEERGDTIVP
                     VQNFVLPATYAGPQRNTRLRRMILTRMQKAGRVYAPAPRVIQERCIGCGQCVRICPKK
                     AIRLTKGKAEFDLARCIRCYCCHEMCQARAIDLKRGVIGELLHTLIR"
     gene            complement(2721..4136)
                     /locus_tag="O0X37_01645"
     CDS             complement(2721..4136)
                     /locus_tag="O0X37_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK61665.1"
                     /translation="MNIIENPLYQKLLLTAVAIGVVMDGLDGSIVNVVLPVIAADFGT
                     DTGTIAWVVITYLLMMAGFLLVFGKLADRGHIRKIFLGGFVIFTAGSAACGLSPDLYC
                     LLGARMFQGIGAAMIAAAAPMLCVKCLPARMLGLALGVLTMASSIGFAAGPALGGILT
                     HYLSWHWIFLINVPIGIAAVPFALRVIPKDGTTEKKPFDLTGAALLFGMMTFGIYALE
                     RLPHLGVSNPQILACTVLCIVFAVLFVFLELRTPTPLINVRVFAAWQFSAVVVAFLII
                     NVVYMGVLYLLPFYLSTGMAFDTAVSGIYLLIPPALTAVLGIPIGKWSDRTERRSFSI
                     GACVVLVLFNLIYAVILPEMGIVPLLCALVLMGVLWGLAGGPAASRIVETAPEGERGT
                     GSSLMAVCIYLGSVIGTALYATIFTFATSPDGTIVAFADLPVATFLAGFHFTILSGIV
                     LGVLAVVLSVLVREQKRGELQ"
BASE COUNT         1107 a         1145 c         1075 g          919 t
ORIGIN      
        1 atatggacgg ctgtatccgc agcgttctca actttgtaca aaaacaggaa aaggcatgat
       61 ttcggtaatc gttcccacgt ttaatgagga ggagggtgtg gaggcctgcc tcaaaagtct
      121 ctgtgatcag acactgcccc gcgatcagta tgagatcatc gttgttgacg gtaactccaa
      181 agacaaaacc cgtgagattg cggaaaagta cgcggacaag gtgtttattc agacgagcaa
      241 gaaggtcggc ggtgcccgca acgacggtgc gatggctgcg aagtatgata tccttgcaac
      301 caccgatgcg gactgtttcc ttccccgcga ctggctggaa aaaattctcg ctgactttgc
      361 caagtatccg gatgcatcaa cgatttacgg cattgtgtat ccgcttgagc cgggtatcaa
      421 acacaagctt tcgctgctcg gttacaacat cgtgtcccgt atcggttact ggacgggaac
      481 gatctacatg acgctcggat gcaacacggc gtttcgaaag gatcttttca tggaggccgg
      541 gatgtacatt acagcggatg caggtgatga ctttgaaatt gcacgccgga tgaaaaaata
      601 cggacgagtc cggctggata cgaatctgaa ggtaggattc tcgatgcggc ggtacattga
      661 gtttggggcg ataaagtcga tttaccagtg gctgtatatt gtggcgcacg gcggatcttc
      721 ggagaagtac cagtacgccg gaaaagagta cgggaaaaaa taatcttttt tcagctggtc
      781 agccgccgct ggtagggtcc gatgagtctg ctgaccgttt ttgcaacaag gccgacacag
      841 atcgcagctg cgatggttcc ttcccggaca ccgtagagac cggagaggaa tgcaaaggag
      901 agcagcacgg caacaaggac cagggaaacg tctgtgatca ccttcatgtt gccgaatttg
      961 atcgggagaa cagtgcagat ggagaggatg agtccttctc caggaagcgg gatgaggttt
     1021 gcagttacct caaggcttac accaataccg agaatgatga ttcctgcaat acagatcatc
     1081 cactgttgac cgtagtctgc ataggtcagt ccgtcaagtc cccagagaca cagatcaata
     1141 agccagccga agagtgtgac cacggcaagc tgtaagagct gtatcggttg aaactgcctc
     1201 cgcatgatga ggatctcccc gacaagcaga atggcgttga tgagaatggt tgcggtacct
     1261 acactcatct cagacatcag gctgacaaca tagggtacgc tggagatagg agatgttcca
     1321 agatctgctt tgatggagag ggcaacaccc actgccatga cacccagtgc aatgcagagg
     1381 aggagggtac gtttgagaat ggatcttcgt gttggtgtaa gcattgcgga tttcgcccgt
     1441 tgtgtggtag aaatatttgt ggtttgggaa tttacgtgtt gtttctccaa aacctaccca
     1501 tcccacggag agtatcattc ctctgccgcg tccaagctac ccatctgaga aaaaacacgt
     1561 gatttacgaa agtgagcttc accggattag ggtgtgaagc agttccccaa taacaccccg
     1621 tttcagatca attgcccgtg cctgacacat ctcatggcag cagtaacagc ggatacagcg
     1681 ggcaagatca aactctgcct tcccttttgt aagccgaatc gcctttttcg ggcagatacg
     1741 aacacactgt ccgcacccga tgcaccgttc ctgtatcaca cgcggtgccg gtgcatatac
     1801 tcttcctgcc ttctgcatcc gggtaagaat catcctgcgg agccgggtat tcctctgtgg
     1861 tccggcatat gttgcaggca gaacaaaatt ctgtaccggc acaatcgtgt ctcctctctc
     1921 ctctaccgta tccagcagac cacgccttcg tgccgcagca gttgttgcaa tctgatccgg
     1981 atccatcccg atcaaccgct gtgcagaata atccaatccg tacaccgaat tagatgccag
     2041 tacatacccg cagaactttg gatcaccccc catcggtccg ttcccttcca ttccgaccac
     2101 cgcgtccatt atcacaaaat ccacaccgat cagctcgttt aaatccacca gcatctccga
     2161 aaaatcctgt tgatccacga accgcgagtg aaacaccggc ttgtccagac cgggtaccac
     2221 cccgaatgtg tttttcactg cgccggagaa ctttgtaaac atatgcgtct tcagcttaca
     2281 aacggagatt atcacatccg cattctgggc aggagtaata acggtaaacc gtttcattac
     2341 tgtaccgttt ggacacggga cctccgcaga tccggtgtca aacgaaagtt ctgctccagt
     2401 ctcttctgcc acagccgcca tcccggaccg ttcataaata cgccggagag tctttggcgt
     2461 gtacaaatgt cccgcccccg gactgtctgc aataatcggt tgccctcctg catccagaac
     2521 catccggcac actgcacgca caatttcggg atgtgccgtc accgatgagt cagctcccag
     2581 atccgacaac aggttcggtt tgatcaaaac acgttttccc cgaaccggcg gaagccctcc
     2641 tgcggcattt actgcttttt caaccgctgt caaaaccgca tcacgatcgt acgaaccaca
     2701 gcacgccaca ccaacaaccg tcattgcagt tctccccgtt tttgttcacg taccagaacc
     2761 gacagaacca ctgcaagtac tccaagcact atccctgaaa gaattgtgaa atggaatcct
     2821 gcgagaaatg ttgcaaccgg cagatccgca aacgccacaa tcgttccgtc gggggacgtt
     2881 gcaaatgtaa agatcgttgc atacagtgcc gttccgatga cactcccaag atagatgcag
     2941 accgccatca gagacgaacc ggttccacgc tctccttccg gcgcagtctc gacaatcctg
     3001 cttgccgcag gtccgcctgc aagaccccag agaacaccca tcagcaccag ggcacaaagc
     3061 agcggcacaa ttcccatctc cggcagaatt accgcataga tcagattgaa cagcaccaga
     3121 accacgcagg cgccaattga aaaactcctg cgctcagtac gatccgacca tttgccgatt
     3181 ggaatcccga ggactgcggt taatgcggga ggaatcagaa gatagatccc gcttactgct
     3241 gtgtcaaatg ccattcccgt actgagatag aacggcagaa gatacagcac gcccatatac
     3301 accacattga tgatcagaaa cgccacgact accgctgaaa actgccatgc ggcaaatacc
     3361 ctgacgttaa tcagcggtgt tggtgttctc agctccagga acacaaacag taccgcaaac
     3421 acaatgcaga ggacagtaca tgccagtatc tgcgggttgg atacgccaag atgcggcagc
     3481 cgttccagtg cataaatgcc aaaggtcatc atgccaaaca gcagtgccgc tcccgtgaga
     3541 tcgaacggct tcttctcggt cgtaccgtcc ttgggaatta cccgcagtgc aaacgggact
     3601 gctgcaatac cgatggggac gttgatcaaa aagatccagt gccaggagag gtaatgcgtg
     3661 agaatcccgc cgagcgcagg acccgcggca aatccgatcg agcttgccat cgtgagaact
     3721 ccgagagcaa gaccgagcat tcttgctgga agacatttga cgcagagcat aggagcagcc
     3781 gcggcgatca tggctgcacc gattccctga aacattcgtg cgccaaggag acagtacaga
     3841 tccggggaga gaccacaggc agctgaccct gcggtgaaga tgacgaatcc gccgaggaag
     3901 attttgcgga tgtgaccacg gtcggcgagt tttccaaaca ccagcagaaa tcctgccatc
     3961 atcagcaggt aggtgatgac aacccaggcg atggttccgg tgtcggtacc gaagtctgcg
     4021 gcaatgaccg ggagaaccac attgacgatg gatccgtcaa ggccgtccat gacgacaccg
     4081 atcgcaacgg cggtgaggag aagtttctgg taaagcgggt tttcaatgat attcatagga
     4141 ggtgaccacc ggatttttga gatgtagtgc tgcctgcatc atcatatatc ttttcgtcag
     4201 tactccaaga aaaaaaggtt agtcggtttt tgcaatctgc tgcatg
//
LOCUS       DALUAJ010000073         3333 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_12900,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000073 DALUAJ010000000
VERSION     DALUAJ010000073.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12900"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(114..791)
                     /gene="radB"
                     /locus_tag="O0X37_01650"
     CDS             complement(114..791)
                     /gene="radB"
                     /locus_tag="O0X37_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair and recombination protein RadB"
                     /protein_id="HJK61666.1"
                     /translation="MEEGLTKKISTNVLGFDRLLGGGLEPRMITQFVGEAGSGKSTLC
                     LVSAVAVLRAGGGVVYIDSEGFSVERFSQIAGENTEEFAKRIYIEEPMTFAEQGIMIA
                     GCESLLRAGKVGLIVVDSATALYRLEQMETKEALSMLSHQMMVLLALAKRFDVPVVIT
                     NQVFMDLERHKLSGLGGTALAHISKAIVRVEKHDGFRRAVVTKHRSQPEGVSWDFVIT
                     GDGVRDR"
     gene            complement(776..1948)
                     /gene="larC"
                     /locus_tag="O0X37_01655"
     CDS             complement(776..1948)
                     /gene="larC"
                     /locus_tag="O0X37_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HJK61667.1"
                     /translation="MRLLVIDPGFGAAGDMMCGALLAAGADRNTVLCAMRSVAPDLSV
                     SQVVRCGMPALYVQTHTGPAHRTLDEVLAIVRAADAPAAAVSLAERVFSRIAAAEEVV
                     HGTHHVHFHEVGADDAIADVLGACTALVTLGVDAVHILPLSVGSGTIVCAHGTMPVPA
                     PATAEILRNSDLQVMIGGFAGELCTPTGAALLAEFSASFGTKDHVGKIVSTGCGAGTR
                     DPAEYPNVLRAMVLVTEDQFFAPAVDVLETNVDDVSGELLSDTVSSLMSAGARDACLV
                     PVVMKKGRPGYIIRVIAMPEDSERLARLLARETGSLGIRCTPMIHRFVADRRISSETV
                     IVNGNAFTVDVKTAFMDGVPYSRKAEFDQVRDAANAAGISVRDMKRVIEEEAWKKD"
     gene            2068..>3333
                     /locus_tag="O0X37_01660"
     CDS             2068..>3333
                     /locus_tag="O0X37_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJK61668.1"
                     /translation="MPEISLKVDSAYPEDQGSGKARLDPETMQKLGILPGQLVVIEGK
                     RKTVAKVWRAMTADWKQEKIRIDKFTRINAGVNPGDRVLVRPVEEQIEADRVCLIPPI
                     RMPQNYDAEPEEIVESLINFPITEGDILPIITNLPNTYLEFKIASIEPEGACIISRNT
                     DIEISDEGDLEGFEGTKQISYEDIGGLKRELRRVREMIELPIRHPELFETMGIDPPKG
                     VLLYGPPGTGKTLIAKAVANESGAHFISIAGPEVISKYYGESEQRLREVFEEAEANAP
                     AIIFIDELDSIAPRREDVSGEVERRVVAQLLTMMDGITNRGQVVVVGATNRPDAIDPA
                     LRRPGRFDREIEIGVPSEADRQEILQIHTRGMPFEGAAHIKELKLHGTEKKIEAAETE
                     YNQNREKLLAQLASLSKGFVGADLAALARE"
BASE COUNT          927 a          922 c          782 g          702 t
ORIGIN      
        1 atggaacacg gccggagatt ctgtttgaaa ccgcggacta tgtgattgaa aacatctgtg
       61 aagcagcacg gataattcag gaaatggcag tgaacagtga aacagagtag gcgttaccta
      121 tccctaacac catccccggt aatgacaaag tcccacgaaa ctccctccgg ttgcgaacga
      181 tgtttagtca ccactgcacg ccggaaccca tcatgcttct ctacccgcac aattgctttc
      241 gaaatatgtg caagagcagt cccgccaagt ccgctcagtt tgtgccgctc cagatccata
      301 aacacctgat ttgtaatcac caccggaaca tcaaaacgtt ttgcaagagc aagtaaaacc
      361 atcatctggt gagaaagcat ggaaagtgct tctttggtct ccatctgctc cagacgatac
      421 aaagcagttg ccgagtcaac cacgataagc ccgacttttc ccgcacgcag aagagactca
      481 cacccggcaa tcatgatccc ctgctccgca aatgtcatcg gctcttcaat atagatacgc
      541 tttgcaaact cctcggtgtt ctctccggca atctgggaga aacgttccac cgaaaatccc
      601 tcggaatcaa tatagaccac cccacctccg gcacgcagta ccgcaaccgc agagacaaga
      661 caaagagtac tctttccgga acctgcctct cccacaaact gtgtaatcat ccgcggctca
      721 agtccgccac ccagaagtct gtcaaatccc agaacattcg tggaaatttt tttcgttaat
      781 ccttcttcca tgcctcctcc tcaatcacgc gcttcatgtc ccggacagaa atccctgctg
      841 catttgcggc gtcccgtact tgatcaaact ctgctttgcg tgagtagggt accccgtcca
      901 tgaatgctgt tttgacatcc acagtaaatg cattcccgtt cacgatcacc gtttctgacg
      961 agatgcgacg atctgccaca aaccggtgta tcatcggcgt gcaacggata ccaagacttc
     1021 ccgtttcccg ggcaagcagt cgtgcaagcc gttcagaatc ctctggcatt gcaatcaccc
     1081 ggataatata gccaggccgt ccctttttca tcaccactgg caccaaacat gcatctctgg
     1141 cacctgcact catcagagat gacacagtat ctgagagaag ttctcctgac acatcatcca
     1201 cgtttgtttc cagcacatca acggcgggtg cgaagaactg gtcttctgta acaagtacca
     1261 ttgcccgcag gacgttcggg tactctgccg gatcacgggt acctgcacca cagccagttg
     1321 aaacaatctt tccaacatga tccttcgttc caaaagacgc ggaaaactct gcaagaagtg
     1381 ctgcacctgt cggcgtgcac agttcaccgg caaaaccgcc gatcattacc tggagatccg
     1441 agtttcgtaa aatctcggcg gttgcaggag caggaaccgg catagtcccg tgggcacaaa
     1501 ctattgtacc ggatccaacc gacagcggca gaatatgtac tgcatccacc ccaagtgtca
     1561 caagtgccgt acatgcccca agcacatctg caatcgcatc gtcagcacca acttcatgga
     1621 aatgcacgtg gtgtgtacca tgaacaacct cttcagcagc agcaatcctg gaaaataccc
     1681 gttcggcaag agaaacggca gcggcaggag catctgctgc acggacaatc gccagtacct
     1741 catcaagtgt cctgtgcgca ggtccagtgt gggtctggac atatagtgca ggcataccac
     1801 accgcacaac ttgtgagacc gatagatctg gagctactga acgcatggcg caaagaacag
     1861 tattccgatc agcacctgca gcaagaagtg cgccgcacat catgtcaccg gcagcaccaa
     1921 accctggatc aatcaccaac agacgcatag aagaatcttg aataatactc ttgcattccc
     1981 ggaataaaat tctccccatt agacagatga aatactgctc cctccagaat attattaatc
     2041 aattccggaa cactgtacta ctcaattatg ccggaaatat cactcaaagt cgacagtgcc
     2101 tatcctgagg atcagggatc aggaaaagct cggctcgacc cagagactat gcagaaactt
     2161 gggatactcc ccggccagct tgtcgtcatc gaaggaaaga gaaaaacggt tgccaaagtc
     2221 tggcgtgcca tgactgcgga ctggaaacag gaaaagatcc gcatcgataa gttcacgcgg
     2281 ataaacgcag gggtcaaccc tggagaccgt gtacttgtcc gaccggttga agaacagatc
     2341 gaagcagacc gtgtgtgtct cattcctcct atccgcatgc cccaaaacta tgatgcggag
     2401 ccggaagaaa tcgttgagtc tcttatcaac ttcccgataa cagaaggaga cattctaccg
     2461 atcatcacca acctccccaa cacctacctt gagtttaaaa tagcatcaat tgaaccggaa
     2521 ggtgcgtgca tcatctcccg taatacggac atcgagatat ccgatgaagg agatctagaa
     2581 gggttcgaag ggacaaaaca gatcagctat gaggacattg gcggacttaa aagagaactg
     2641 cgccgcgtcc gtgagatgat tgaactgccg attcgtcacc cggaactttt tgaaaccatg
     2701 ggtattgacc caccaaaagg agttctgctc tacggacctc cgggaacagg aaagaccttg
     2761 atcgcgaagg ccgtcgcaaa tgaaagcggt gcacacttca tatcaattgc aggtcccgag
     2821 gtcatctcta aatattatgg tgagtccgaa caacggcttc gagaggtctt tgaagaggct
     2881 gaagcaaacg ctccggcgat tattttcatt gatgaactag actcgattgc tccgcggcgt
     2941 gaagatgtat ccggagaagt agaacgacgt gtggttgccc aacttctgac tatgatggat
     3001 ggaattacca accgagggca ggttgttgta gtgggggcaa caaacaggcc ggatgcgatc
     3061 gaccctgcgc tccggcgccc cggaagattc gatcgtgaga tagagatcgg tgttccgtcc
     3121 gaagcggaca gacaagaaat cctgcagatc catacgcgcg ggatgccgtt cgaaggggcc
     3181 gcgcacatca aagagctgaa gcttcacgga acagagaaaa agatcgaagc agcggaaacc
     3241 gagtataatc agaaccggga gaaacttctt gcacagcttg catccctgtc caaagggttt
     3301 gtgggagcgg atctggcagc ccttgcccgt gag
//
LOCUS       DALUAJ010000074         1921 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_13110,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000074 DALUAJ010000000
VERSION     DALUAJ010000074.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13110"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            88..915
                     /gene="dapF"
                     /locus_tag="O0X37_01665"
     CDS             88..915
                     /gene="dapF"
                     /locus_tag="O0X37_01665"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HJK61669.1"
                     /translation="MTIAFTKLHGNGNDFILIDEMAGTVIPDDMKAQFAVSYCDRRFG
                     IGGDGVLFISPSDSADVRMRLFQPDASEAEMCGNGIRCLAKYAFDNNYTKTKLFKVET
                     LAGVLTVRAGYDNEGNFSATIDMGVPAYDAASIPATGSGDFVAEIEGMTVYAANTGVP
                     HAVIFVDDVEALDLDAVAPKIRHNSVFPKGTNVNFVQVTGPSAITIRTFERGVEGETL
                     SCGTGSTASALISAKIGKVSGDVVHVDTVGGPLDIGVGERATMTGPAETVFVGEIPF"
     gene            complement(995..>1921)
                     /gene="pheA"
                     /locus_tag="O0X37_01670"
     CDS             complement(995..>1921)
                     /gene="pheA"
                     /locus_tag="O0X37_01670"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HJK61670.1"
                     /translation="VLHASIDKLYVGSEEFLAEIEAGLGASKISAPKVGYQGVAGSFG
                     EQAAREYFGETASTITNYKEFEDVFLALEQGEIDYGVLPIENSTAGDVLEVADLITQC
                     NLYVVGEHIIRVRHNLLGVPGASTKNVREVYSHPQAISQCRDFLRHHSEINAYPYANT
                     ALAAKFVAETHDPAKASIGSLRAAELYGLDILEKNIQTAKNNYTRFVILSRHLEITRQ
                     SDKISLVFSTAHKSGALYNILAHFAYNGLNLLKIQSRPKRGTPWEYIFFLDVAGNLED
                     ANVLIALGKVREQSSWFKLIGNYPHCHHTEKE"
BASE COUNT          439 a          438 c          527 g          517 t
ORIGIN      
        1 ggggcatcct ggattgcaac ccacctcaca tccgcaggtt ctgatgtgtg atcttcaagt
       61 tttctgaaga ataatactgt aagtacaatg acgatcgcat ttacgaagct tcacggcaac
      121 gggaatgatt tcattctgat cgatgagatg gcaggcacgg ttatcccgga tgatatgaaa
      181 gcgcagtttg cagtgagcta ctgtgaccgc aggtttggta tcggtggtga cggtgttctg
      241 ttcatctctc cgtcggatag cgcggatgta cgtatgcgcc tgttccagcc ggatgcaagc
      301 gaagcggaga tgtgcggcaa tggtatccgg tgtctggcaa agtatgcgtt tgacaacaac
      361 tatacaaaga cgaaactgtt caaagtagaa acgcttgcag gtgttctgac tgttcgtgcc
      421 ggatatgata acgagggcaa cttctctgcc acgattgaca tgggtgtccc tgcctacgat
      481 gcagcatcga tccctgcaac cggctctggt gatttcgttg ccgagattga aggaatgact
      541 gtgtatgctg caaacacagg tgttccgcat gcggtcattt ttgtggacga tgttgaggcg
      601 cttgaccttg atgcagttgc gccaaagatt cgccataact ccgtattccc gaagggaaca
      661 aatgtgaatt ttgtacaggt gactgggcct tctgcaatta cgatccggac atttgagcgg
      721 ggagttgagg gagaaacact ctcgtgcggt accggttcaa ccgcatccgc cctcatctct
      781 gcaaagattg gaaaagtttc gggagatgtg gtgcatgttg acacagttgg cggccccctg
      841 gacatcggtg tcggtgagcg tgcaactatg accggacctg ctgaaacggt atttgtcggt
      901 gagatcccgt tctaactttt ttttgaaaaa attatttttt ggatgggtcg ttgccaaact
      961 gcatgattag agcgtatgca gcgcagatat tgcattactc tttttctgtg tgatggcagt
     1021 gaggatagtt tccgatcagt ttgaaccagc tgctctgttc gcggactttt ccaagagcaa
     1081 tcaatacgtt tgcgtcctca agatttcctg caacatcaag gaagaagatg tactcccatg
     1141 gtgtacctcg tttgggacgg gactggatct ttaacaggtt cagcccgttg taggcaaagt
     1201 gggcaagtat attgtagagt gctccgcttt tgtgagctgt ggagaagaca aggctgattt
     1261 tatccgactg tctggtaatc tccagatgcc gcgaaaggat gacaaatcgc gtgtagttgt
     1321 tctttgcagt ctggatattt ttttccagaa tgtcaagacc gtagagttct gcggcgcgca
     1381 ggctgccgat ggaagccttt gcgggatcgt gtgtctctgc aacaaacttt gcggcgagtg
     1441 ctgtgttcgc atacgggtag gcgttgatct cactgtgatg ccggaggaag tcacggcact
     1501 ggctgatggc ctgcggatgg gagtacacct cgcgaacgtt tttggttgat gcacccggga
     1561 caccaagcag attatgacga acgcggatga tgtgttcgcc gaccacatac agattgcact
     1621 gtgtgatcag atccgcaacc tcaagcacat ctccggcggt tgagttctca atcggcagaa
     1681 caccgtaatc gatctcgcct tgttcaagcg ccagaaaaac atcctcgaac tctttgtagt
     1741 tggtgatcgt cgatgcagtt tccccgaagt actcgcgggc ggcctgttct ccaaaagaac
     1801 ctgcgacacc ctgatatcct acttttggtg cagagatttt tgaggcacca agtcctgctt
     1861 cgatctctgc cagaaactct tcgctgccga catacagttt gtctattgag gcatggagta
     1921 c
//
LOCUS       DALUAJ010000075         1713 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_13230,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000075 DALUAJ010000000
VERSION     DALUAJ010000075.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13230"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1713)
                     /locus_tag="O0X37_01675"
     CDS             complement(<1..>1713)
                     /locus_tag="O0X37_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HJK61671.1"
                     /translation="VVMGLRGHVVELDFTKGYSNWRSEEHPPRSLINAGIEKHPTEKK
                     IVALMQKHAKKADRITIATDYDTEGELIGMEAYELVRAVNQKATVDRARFSAITKDEI
                     TKAIADAKEIDFNLAAAGETRQVIDLVWGASLTRFLSIAAHRGSDNILSVGRVQSPTL
                     AMIVDREKEIEAFVPEKYWMLSLTAKVAGEEIASRHVHGRFTVKAEADAAFAGTRDPV
                     IVREVITGKKNDKAPTPLDTTALIVGAGRLGLSAASAMSKAEDLYMRGFISYPRTDNT
                     AYPKSLNIRQHLKMFAGGEFERDANYVLTHMRAAPTRGKKETTDHPPIYPTSKGTREE
                     IGDEATWKLYEFIVRRFFATLSPDAEWKTLKVNLTADKEPYTITGGRLLVPGWRAVYP
                     YSKAEETVLPEFSVGERLELVDKNCEEKETQPPARYSQSRLIQRMEELGLGTKSTRHE
                     VISKLIGRKYIEGNPMKPTVVGRAVTESLEKFADTITEPTMTKTLEESMEDIAAGKKT
                     MNAVLTESRMMLSSIFDDLEANKEAIGQDIMDRTREEQTVGQCPICGAPLRIRRVGTS
                     QFIGC"
BASE COUNT          331 a          467 c          463 g          452 t
ORIGIN      
        1 acatccgata aactgggagg ttcctacacg ccggatccga agcggtgcac cgcagatcgg
       61 acactgacca acggtctgct cctcacgtgt gcggtccatg atgtcctgac caattgcctc
      121 tttgtttgct tccagatcat caaagatgga ggaaagcatc atccttgact cggtcagaac
      181 cgcattcatg gtctttttcc ctgcggcgat gtcctccatc gactcttcca gcgttttagt
      241 catcgtcggc tcggtgatgg tatccgcaaa cttttccaga gactcggtaa ccgcacgacc
      301 gacaacggtc ggtttcatcg ggttgccttc gatatatttt ctgccgatca gtttgctgat
      361 aacttcatgc cgtgtacttt tggtaccaag gccgagttct tccatccgct ggataagtct
      421 gctctgtgag tagcgtgccg gaggctgggt ctctttctcc tcacagtttt tatcgacaag
      481 ctcaagccgt tctcctactg aaaactccgg cagaactgtc tcttcagcct tgctgtaagg
      541 atacaccgca cgccatccgg gaacaagcag tcgtccgccg gtgattgtat agggctcttt
      601 gtctgcggtc aggtttacct tcagcgtctt ccactcggca tctggggaaa gggttgcaaa
      661 aaatctgcgg acaataaact catagagttt ccaggtggct tcatcgccga tctcctcgcg
      721 ggtacctttg gaggttggat agattggcgg gtggtcggtg gtctccttct ttccacgggt
      781 cggtgctgcc cgcatatgtg tcagcacata atttgcatcg cgttcaaact caccgccggc
      841 aaacattttc aggtgctggc ggatgttgag gcttttaggg tatgcggtgt tatcggtacg
      901 gggataggag ataaatcccc gcatgtaaag atcctctgct ttgctcatcg cggacgcagc
      961 ggagagacca agacgtcctg ccccgacaat aagggctgtg gtgtcaagcg gggttggtgc
     1021 cttgtcgttt ttcttgccgg tgatgacctc ccgtacgatg accggatcgc gtgtcccggc
     1081 aaatgccgca tcagcctctg ctttgacagt gaatctccca tgcacatggc gggatgcaat
     1141 ctcctcgccc gcaacctttg cggtgaggga aagcatccag tacttctccg gtacaaatgc
     1201 ctcgatctct ttttcccggt caacgatcat ggcaagcgtc gggctctgca cgcggccgac
     1261 ggagagaatg ttgtcggaac cgcggtgtgc ggcaatggag aggaaccggg tcagggatgc
     1321 tccccagaca aggtcaatga cctgacgggt ctcaccagct gctgcaaggt tgaagtcgat
     1381 ctcttttgcg tcagcaattg ccttggtgat ctcgtccttt gtgattgccg agaaccgggc
     1441 acggtcaacc gttgccttct ggttcaccgc ccggacgagt tcataggcct ccatgccgat
     1501 gagttctcct tcagtatcgt aatcggttgc aatggtgatg cggtcggctt tctttgcatg
     1561 cttctgcatg agagcaacga tctttttttc cgtcgggtgt ttttcaattc ctgcgttaat
     1621 gagggaacgc ggcgggtgct cctcgcttcg ccagttactg tagcctttgg tgaagtcaag
     1681 ttcgaccaca tggccgcgaa gacccatcac cac
//
LOCUS       DALUAJ010000076         1564 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_13342,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000076 DALUAJ010000000
VERSION     DALUAJ010000076.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13342"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..922)
                     /locus_tag="O0X37_01680"
     CDS             complement(<1..922)
                     /locus_tag="O0X37_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HJK61672.1"
                     /translation="MDSERRYYVDEEEAKIVREIFERYASGETVADIIRDLNRRKLTT
                     SLGKPFNKNSVHRILNNRRYIGYYLYKGQETPGGMPRIIEDELFERVHYILDRNKKAP
                     ARARGREEYLLTTKLFCGYCREMMTGYGGTGKSGKTYHYYACNNFKRRKCKKKVISKE
                     KIEDRVVLECRKLLTDSNIERIAASVSEVCESNRDTASIKRIRAAIQEADTAIENLWK
                     ALERGQAADMITERIEKRQHEKEELQAQLAVEMAKQVTLTAPQVRAYLYSLKQGDKND
                     ENIKRGIINIFLRAVYLYDDRFTLVLNGSDT"
     gene            complement(924..1391)
                     /locus_tag="O0X37_01685"
     CDS             complement(924..1391)
                     /locus_tag="O0X37_01685"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HJK61673.1"
                     /translation="MNVVIYARYSSHSQTEQSIEGQLHTCYEYARNNGYLVVGEYIDR
                     AQSGTTDSRAEFQRMIADSDKHTFEGVLVYQLDRFARNRYDSAINKAKLKKNGVRVIS
                     ARENISDDASGILVEGVLESMAEYYSAELSQKVRRWRSMHKSFGQWQQPWFRL"
BASE COUNT          334 a          416 c          369 g          445 t
ORIGIN      
        1 gagtgtcgct accgttcaaa accaaggtga accgatcatc atacaaatat accgcgcgga
       61 ggaagatatt gatgatccca cgtttgatgt tctcgtcatt cttgtcccct tgcttgagcg
      121 agtagagata ggctctaacc tggggtgctg tcaaagtcac ctgctttgcc atctcgacag
      181 ccaactgtgc ttgcagttcc tctttttcgt gctggcgttt ttcaatccgc tcagtaatca
      241 tgtcggcggc ctgtccccgc tccagcgcct tccagagatt ttcaatggcg gtgtctgctt
      301 cctggatggc agctctgatg cgtttgatgg atgctgtgtc ccggttggat tcacagacct
      361 cggataccga agctgcgatc cgctcaatgt tgctgtctgt cagcaacttg cggcactcca
      421 acacgacacg atcctcaatt ttctctttac tgataacctt ctttttgcat ttgcgccgtt
      481 tgaaattatt gcaggcatag tagtgatagg tcttgccgga ttttcctgta ccaccgtaac
      541 cggtcatcat ctcccggcag tagccacaga acagctttgt ggtgagcaga tactcttccc
      601 gccctctagc tcgggcaggt gcttttttgt tgcgatcaag gatatagtgg acacgctcaa
      661 acagctcatc ttcgatgata cgaggcatac cgcccggcgt ttcctgtcct ttgtagaggt
      721 aatacccgat gtaacgccgg ttgttcaaaa tgcggtggac gctgttttta ttaaagggtt
      781 tgccaagaga agtcgtgagc tttctgcggt tcaaatcacg gataatgtct gcaacggttt
      841 ctccactagc ataccgttca aaaatctccc gaacgatctt tgcttcttcc tcatccacat
      901 agtaccgacg ttcggaatcc accttatagc ctaaaccagg gttgctgcca ttggccaaaa
      961 cttttgtgca ttgatctcca cctgcgaacc ttttgggaaa gctccgctga gtagtattca
     1021 gccatacttt ccagaacacc ttccaccaga atgccgctgg catcatcgct gatgttttcc
     1081 cgcgcggaga tcactcgcac accgttcttt ttcagttttg ccttgttgat ggcactgtca
     1141 tatcggttgc gggcaaaacg gtcaagctgg taaaccagaa caccctcaaa ggtatgcttg
     1201 tcactgtcgg caatcatcct ctggaactcg gcacggctat cggtggttcc gctctgggcg
     1261 cggtcaatat attctccaac aacgagatag ccattgtttc tggcatactc atagcaggtg
     1321 tggagttgtc cctcgataga ctgctcggtc tggctgtggc tggagtagcg ggcgtagatg
     1381 acaacattca tttcttcacc tccccgagca tttgcagcag ggtctccttg agccgcgcat
     1441 tcatggggga tgcggggtca aagcacatct ccacaaactt ctgcatatgg agatcattct
     1501 gctggatttt aggcttatac tcatacagct taccctgagg attgtccgtg cgtccaaaga
     1561 tata
//
LOCUS       DALUAJ010000077         2194 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_13496,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000077 DALUAJ010000000
VERSION     DALUAJ010000077.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13496"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="O0X37_01690"
     CDS             complement(<1..486)
                     /locus_tag="O0X37_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJK61674.1"
                     /translation="MTTPFITIKDLCMDFPANPEGPSDTTPCAAAQESVRVLDNINLE
                     IAEGEIVGIIGRSGCGKTVLIHLVRGIDQPPTSGQIIYHVAVCPKCGRIELPGHAGTA
                     CSYCGAVLEARDVDFWAPENETLKTRIMARTAIMFQRTFALYGDDRVIEHVLRALDDI
                     DY"
     gene            complement(607..>2194)
                     /locus_tag="O0X37_01695"
     CDS             complement(607..>2194)
                     /locus_tag="O0X37_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-CASP ribonuclease aCPSF1"
                     /protein_id="HJK61675.1"
                     /translation="GIVIGKNGSTLRDITMKVGWTPKVVRTPPIPSVTIKQVRQFLRE
                     TRDERKEFLRKCGRRIHRDSIYTGRDKEQWLRITTLGCCRQVGRAAFLLTTPESKVLI
                     DCGESPGATGSASSPFLNVPEIYPFTSLDAVVLTHAHLDHSAYIPLLYRYGYDGPVYT
                     TPATRDTAAMLQLDYLDVNNKEDKVPPYSSNEIREFVKHTIALGYGDVTDIAPDMKLT
                     LHNAGHILGSAIAHFNVGNGLYNIAFTGDFFYSKSRLFNPAVSQFPRLEALVMESTYG
                     GSEDFSPSRKDAEDHLYEVINTTLARGGKVLIPAFAVGRSQELMLALEEGFRNQRIPQ
                     CKIYLDGMIKEATALHTAYPEYLNNDLRNLIFRDNYNPFLAECFEQVDSQEKRQQVIF
                     SEDPCVIISTSGMLNGGPVLEYLGNLAESEKNMLIFVGYQAEGTYGRRIQKGWREVPV
                     GKKGSIVINMEVQTVEGFSGHADRRQLMNYVQYLQPKPERVFTIHGEESKTIDLASSI
                     YKKYHIQTVAPQNLETYRLV"
BASE COUNT          546 a          544 c          556 g          548 t
ORIGIN      
        1 atagtcgata tcatccaagg cccggaggac gtgttcaatg acgcgatcat ctccgtacag
       61 ggcaaatgtc cgctggaaca tgatcgcagt ccttgccatg atacgggttt tgagtgtttc
      121 attttccggt gcccagaaat ccacatcccg cgcttcaagt acagcaccac agtaactaca
      181 ggctgttccc gcatgaccag gaagttcaat tctgccgcat ttggggcaga cagcaacgtg
      241 ataaatgatc tgtccggatg tagggggctg gtcaatacca cgaacaagat ggatgagtac
      301 ggtcttaccg catccggatc gtccgatgat accaacgatc tctccttcgg ctatctcaag
      361 attgatgttg tccaaaaccc ttactgactc ctgcgcggct gcacagggcg tagtatcgga
      421 aggaccttca ggatttgcag gaaaatccat gcagaggtcc tttatggtga taaacggtgt
      481 cgtcatgcaa acatcctcta taatgctaaa tgtatgtgct ttgaggtgtt attagtgttt
      541 agaagaatgc attctggcgg gattattcac aatgtgacat cccagccttg aaaaagaaaa
      601 aaaagttcaa accagacggt aagtctcaag attctgtggt gcaaccgtct gaatgtggta
      661 tttcttgtag attgaacttg ccagatcaat tgtctttgac tcttcaccgt gaatggtgaa
      721 cacccgctca ggtttgggct ggaggtactg cacatagttc ataagctgac ggcggtctgc
      781 atgaccggag aatccttcca ctgtctggac ttccatgttg atgacaatac tgcctttttt
      841 gccgaccgga acttcacgcc atcctttctg aatgcggcga ccgtacgttc cttcagcctg
      901 atacccaaca aagatgagca tgttcttctc tgactccgca aggtttccaa gatactccag
      961 gaccggtcct ccgttcagca taccgcttgt tgagataatc acacacgggt cttccgagaa
     1021 gataacctgc tgacgtttct cctgtgaatc tacctgttca aaacactcgg caaggaaggg
     1081 attgtagttg tcacggaaga tgaggttccg aagatcgttg ttcaggtact caggatatgc
     1141 agtgtgcagg gcagttgctt ccttaatcat tccgtcaagg tagatcttac actgagggat
     1201 gcgctggttt ctgaaccctt cctcaagagc aagcatcagt tcctgtgatc tgccaacggc
     1261 gaatgcagga atcaacacct taccgccgcg ggcaagagtc gtgttaataa cttcatagag
     1321 gtgatcttct gcatcctttc ttgacgggga gaaatcctca gagcctccat acgtactctc
     1381 cataaccagt gcttccaggc gcgggaactg tgaaacagcc gggttgaaca gacggctttt
     1441 gctgtagaag aagtcaccgg taaatgcaat gttgtaaaga ccattcccaa cattgaagtg
     1501 ggcaatggct gaaccgagga tgtgacctgc gttgtgcagt gtcaatttca tatcaggcgc
     1561 aatatccgtt acgtccccat aaccgagagc aatggtgtgc ttcacaaact cacgaatttc
     1621 atttgaggag tacggcggga ctttgtcctc tttgttgttc acatcaagat agtcaagctg
     1681 aagcattgca gcggtgtcgc gggttgcggg tgttgtgtag accggaccgt cataaccata
     1741 acggtaaagc agcgggatat aggccgagtg atccagatgt gcgtgcgtaa ggacaaccgc
     1801 atcaagcgag gtgaacggat aaatctccgg aacgttcagg aacggtgaag aagcactgcc
     1861 tgtggcccca ggagactccc cgcagtcaat cagaaccttg ctttccggag tggtgagcag
     1921 aaatgccgca cggccaacct gccggcagca gccgagtgtg gtgatacgca gccactgttc
     1981 cttatcacgt ccagtgtata tcgagtcacg gtgaatacga cgtccgcatt tgcgcaggaa
     2041 ctcctttcgc tcgtcacggg tttcgcgcag gaactgacgc acctgcttga ttgtcacaga
     2101 gggaatcggc ggagttcgta caaccttcgg tgtccagcca accttcatcg taatgtcacg
     2161 aagcgtcgat ccgtttttac cgatgacaat accc
//
LOCUS       DALUAJ010000078         3727 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_13822,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000078 DALUAJ010000000
VERSION     DALUAJ010000078.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13822"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(77..787)
                     /locus_tag="O0X37_01700"
     CDS             complement(77..787)
                     /locus_tag="O0X37_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HJK61676.1"
                     /translation="MSVSPDAELATLRTELAALQAYPREKLEEIIADVGFSCTCCGRC
                     CTRSFNGHVFLLSTDAERLRTFAPEMLMPAPDFPYSDTSGNFYVSGYALRTKQNGDCV
                     FLSEEKRCTIYEDRFSICRIYPYMLHREPDRRGHVDWRQISGIDEHGEYDCPISSEDV
                     ERIASEVIAYETAYLEQEFSFHEALIAKFSREGMRFVRRDHDRRFREFMKGDAHAVVY
                     VWDGQIFRREEAVPSDYR"
     gene            complement(941..2041)
                     /gene="mfnA"
                     /locus_tag="O0X37_01705"
     CDS             complement(941..2041)
                     /gene="mfnA"
                     /locus_tag="O0X37_01705"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004837 - tyrosine decarboxylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase MfnA"
                     /protein_id="HJK61677.1"
                     /translation="MEDKGCSREEVLSFLAGLRAEDVHHDHILSSMCTIPHPIAVAVH
                     EMFSATNLGDPGLYPGTAKVEDLLIHSLGELMHLPNAGGYSTSGGTESNLQAIRIAKK
                     LKPEAKNPNIVVPASAHFSFDKTCDMLGLEMRIVPYGESYTVDTEKMADMVDKNTIAV
                     AAVAGTTEYGMIDDVPTVAKIAEECNCFCHVDAAFGGFVIPFLENPPQFDFAVPGVDS
                     ISLDPHKMGLATIPCGSLLLRDPDQLGCLNVDTPYLTVKKQCTLAGTRPGTDVAGAYA
                     VIRHLGREGFRAIVAGCMENTRRLVDGMEACGYHRVVDPIMNVAAFTGGDAPAGWKVS
                     HTRAGHLRFVVMPHVTRDVIECFLADVAAMYS"
     gene            complement(2074..>3727)
                     /gene="ppsA"
                     /locus_tag="O0X37_01710"
     CDS             complement(2074..>3727)
                     /gene="ppsA"
                     /locus_tag="O0X37_01710"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HJK61678.1"
                     /translation="WGLGEAIVSGTVSPDNYVYDRKNKRITTKTVANKSVEIIPDGNK
                     GTKTVPVPESRQNVQVLSDVEVAKLAEFAVISEEHYKVPQDMEWGMVGDTIYILQSRP
                     ITTIQKNHVVSASEKTADSAADGEIILQGYGASPGIASGPVVIVSDAKDTSRVKEGDI
                     MVSPMTNPDMVPAMRKVVGIITDEGGMTCHAAIVSRELGTPAVVGTKKATSLLKEGQI
                     VTIDGEKGLIYDGKLEAASPVTAAVAAPVAAAAPIITATSVKVNVSMPEAAARAAATG
                     ADGVGLLRIEHLIIGMNKTPSWYIKNGKEEEFISELYDGIKTVLDAFRGKPVWVRTID
                     SPTDEFLNMAGGEDEPHEHNPMLGFRGLRRDLRQMAQFKMQAECFKRLWEAGYDNLGI
                     MFPLVQHPREFLAAKEAFRSWGIDVENRVLGIMVEIPASAIIIDEFIKAGVKFCSFGT
                     NDLIQYTLAVDRNNGMIGDMYEPEHPAVMRLIKHCIEQCREAGVECSICGQAGSDPKF
                     VKWLVNQGISSVSSNIDAIPKIRETVAKTERQILLNMAFKKN"
BASE COUNT          931 a         1018 c          880 g          898 t
ORIGIN      
        1 aacgggataa taggtaactg gttccatgag ggagtatatt ggtttacttt ttagaagagt
       61 tatgcggttt tgtcttttac cggtagtcag aaggaaccgc ctcttctcta cggaagatct
      121 gcccatccca tacatacact accgcgtgtg catccccttt cataaactca cgaaaacgcc
      181 ggtcatggtc cctccgcaca aaccgcatac cttcccggga aaattttgca atcagtgcct
      241 catgaaagga gaattcctgc tcaaggtagg cagtctcata ggcaattacc tcagatgcta
      301 tcctctcaac atcttctgaa gagattgggc agtcgtactc cccgtgttca tctataccac
      361 tgatctgccg ccagtccaca tgcccgcgcc ggtcaggttc gcggtgaagc atatagggat
      421 aaatccggca gatggaaaac cgatcctcat agattgtgca tcttttttct tcagaaagaa
      481 acacacagtc accgttttgt ttcgtccgga gcgcgtagcc ggatacataa aaatttcctg
      541 aagtgtccga gtaggggaaa tccggtgccg gcatcagcat ctcaggtgca aaagtacgaa
      601 gccgttctgc atccgtagag agcagaaata catggccgtt aaatgaacgt gtacaacaac
      661 gtccgcaaca ggtacaggag aacccgacgt ccgcaatgat ctcctccagt ttttctctgg
      721 gatatgcctg gagggcggca agttctgtgc gaagtgtggc aagttcggca tctggactaa
      781 cggacatgac tgagaatata cttggctgca aagagatatg agtgtgattg ccgcatgtgg
      841 atgataccaa aaaaggcaac aacgattctg cttatctgat gttccggaaa tcgttagcta
      901 aggcggacag tcaatttact ggtgcctaca caaaaaaaga ttatgaatac atcgccgcaa
      961 catcggcgag gaaacactca atcacatcac gcgtcacatg cggcatcacc acaaaccgca
     1021 gatgtccagc acgcgtatgt gaaactttcc aacccgctgg cgcatcacca ccggtaaagg
     1081 ctgccacgtt catgatcgga tccaccaccc ggtggtagcc gcatgcctcc atcccatcaa
     1141 ccagcctgcg ggtattttcc atacatcctg caacgatcgc acggaatccc tctcgcccca
     1201 gatgccgtat cactgcatac gctcccgcaa catccgtacc aggccgtgtt cctgcaagag
     1261 tacactgttt tttcactgta agatatggtg tatccacgtt cagacagccg agctgatcgg
     1321 gatcgcggag cagcagtgag ccgcaaggaa ttgttgcaag ccccatctta tgcgggtcca
     1381 gtgagattga gtcaacccct ggaactgcaa aatcaaactg cggaggattt tccaggaacg
     1441 gaatcacaaa tccaccgaaa gcagcatcca cgtgacagaa gcagttacac tcctcggcaa
     1501 ttttggcaac agtagggacg tcatcgatca tcccatactc tgtagttcct gcaaccgccg
     1561 caacagcaat agtgtttttg tccaccatat ctgccatttt ctccgtgtcc acagtatagc
     1621 tttcaccata gggaactatc cgcatttcaa gccccagcat gtcacacgtc ttgtcgaacg
     1681 aaaagtgggc agatgccggg accacaatgt ttggattctt tgcctcaggt ttcagttttt
     1741 ttgcaatccg gattgcctga agatttgact cagttccacc cgaagttgag tatcctccgg
     1801 catttggaag atgcatgagt tccccaagag aatggatcag cagatcctcg acctttgcag
     1861 ttcccggata gagaccagga tctcccagat ttgttgcgct aaacatctcg tgtaccgcaa
     1921 ccgcaattgg atgcggaatg gtacacatgg aactgaggat atgatcatga tgcacatcct
     1981 cagcccgtaa accggcgagg aaggagagaa cctcctccct gctgcaccct ttatcctcca
     2041 taaagtagcc ctgaaaaaat tgagtgttga gtattagttc ttcttaaatg ccatattcag
     2101 cagaatctgg cgctcagtct ttgcgaccgt ctcacgaatc tttggaattg cgtcaatgtt
     2161 tgaggagaca ctggagattc cctggttcac cagccatttc acgaacttcg gatccgatcc
     2221 cgcctgacca cagatggaac attcaacgcc cgcctcacgg cactgttcaa tacagtgttt
     2281 gatcaggcgc atcacagccg ggtgttccgg ctcatacata tcgccgatca taccgttgtt
     2341 gcggtccacg gcaagggtgt actggatcag atcgtttgtt ccaaaggaac agaacttgac
     2401 acctgccttg atgaactcat caataatgat agcggatgcg ggaatctcaa ccatgatacc
     2461 aagcacacga ttctctacat caatacccca ggaacggaac gcctcctttg ctgcaaggaa
     2521 ctcacgtggg tgctgtacca gcgggaacat gataccaagg ttgtcgtatc ctgcctccca
     2581 caggcgcttg aaacactctg cctgcatctt gaactgtgcc atctggcgaa gatcacggcg
     2641 cagaccgcgg aaaccaagca tcgggttgtg ttcgtgcggc tcatcctctc cgcctgccat
     2701 gttcaggaac tcatcggtcg gagaatcgat agtacgcacc cagacaggct ttccgcggaa
     2761 agcatcgagc accgtcttga taccatcata cagttcggag atgaactctt cttccttgcc
     2821 gttcttgatg taccagctcg gagtcttgtt catgccgatg atcaggtgct cgattctcag
     2881 cagacccaca ccgtcagcac ccgttgctgc cgcacgtgct gctgcctcgg gcattgaaac
     2941 gtttaccttc actgaggttg cggtgatgat aggtgctgca gctgcaaccg gtgctgctac
     3001 cgcggctgtc acgggtgatg ctgcctcaag cttaccgtcg taaatcagac ccttttcacc
     3061 atcgatggta acaatctgtc cttctttcag aagagaggtt gcctttttcg taccgacaac
     3121 cgcaggagta cccagctcac gcgacacaat tgccgcatga caggtcatac caccttcatc
     3181 agtgatgatg cccaccactt tccgcatagc aggaaccatg tccgggttgg tcatcgggga
     3241 gaccatgatg tcaccctctt ttacacggga ggtgtccttt gcatcggaaa cgataacgac
     3301 cggacctgat gcaattccgg gggaagcacc gtatccctga agaataatct caccgtctgc
     3361 tgcagaatcg gctgtcttct cggacgcgga gacgacatga ttcttctgaa tcgtagtgat
     3421 tgggcgggac tggagaatat agatagtgtc gcccaccatt ccccactcca tatcctgcgg
     3481 aactttatag tgctcctcag agatgacagc aaactcagca agttttgcaa cctcaacgtc
     3541 agaaagaacc tgaacattct ggcggctctc cggaacaggg acagtctttg ttcctttgtt
     3601 accgtccgga atgatctcaa cagatttgtt ggcaacggtc tttgtggtga tccgcttgtt
     3661 cttgcggtca tagacatagt tgtcaggtga cacggtgccg gatacaattg cctcgccaag
     3721 accccat
//
LOCUS       DALUAJ010000079         2259 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_14239,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000079 DALUAJ010000000
VERSION     DALUAJ010000079.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14239"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="O0X37_01715"
     CDS             <1..791
                     /locus_tag="O0X37_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJK61679.1"
                     /translation="SREALAEIVAGHTAGDIAAFGMESADPAVVAANNLKGDADAVFR
                     AVEIVNEVGAKRRDGIPELLPGLNFIAGLAGETAETFAMNEAFLQRILSSGLMVRRVN
                     IRQLMPFEGTRAYEDNTLGQHDRLFHQFKERVRETFDVPMLARVFPVGTVLRDIVIEV
                     SGSVSFGRQMGTYPILCGIPMQIPVGTVMDAAVVSYGQRSVTALPVPIEINRLNAAAL
                     KWLPGVSKKAAAQIAAKKPYADAAAFRSAVSSDAVAPLIELMRF"
     gene            complement(788..1240)
                     /locus_tag="O0X37_01720"
     CDS             complement(788..1240)
                     /locus_tag="O0X37_01720"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HJK61680.1"
                     /translation="MESIRVRPYSSADYPSICELDAPLFAGMGGPVLFRHIEELFPSL
                     FFVAENAEKDIIGYILGGIHLDDPKTGKLIRIGVVQNYQRKECGTKLTEALFSAMKER
                     NVTAVHLTVAETNKPAIAFYQKIGFMQKERIKNYFYPTTPRLVLWKTL"
     gene            1347..1616
                     /locus_tag="O0X37_01725"
     CDS             1347..1616
                     /locus_tag="O0X37_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HJK61681.1"
                     /translation="MKWQISDLFGMSVYSDKAVFLGKVEDVVLDVTNKKISGLALTSV
                     NQNVLDIKNYQGVVIPYRIVKEVGDIILVRHIPGAFKVSEPLFGE"
     gene            1632..>2259
                     /locus_tag="O0X37_01730"
     CDS             1632..>2259
                     /locus_tag="O0X37_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5'-guanylyltransferase"
                     /protein_id="HJK61682.1"
                     /translation="MKDREIFTGLITTVPFILRLDGRSFHHLSRNNGYAKPYDPVFSR
                     CMVQTAQAMLENSGLAPSFAYTFSDEISLYVTSPVFDCRVEKLVSVAAGFASSAFTLF
                     SRSDVPLSFDCRVVPIDEVHLAEYLAWRQAEAWRNHINGYAHKLLMDTGLSATAAQRK
                     LDGMKSSDLHELAFSFGVNLAETPAWQRRGTAVYRSVVEKEGYNPVSQE"
BASE COUNT          521 a          510 c          597 g          631 t
ORIGIN      
        1 catcccgcga agcactcgcg gaaattgttg cagggcacac ggcaggagat attgcagcgt
       61 ttgggatgga gtctgccgat cccgcggtgg ttgcggcaaa taatctgaaa ggtgatgcgg
      121 atgctgtttt ccgggcagtt gagatcgtta atgaggttgg tgcaaagcgc agggacggaa
      181 ttcctgaact tctgcccggt cttaacttta ttgcgggtct tgccggtgaa actgcagaga
      241 cgtttgccat gaatgaggca tttttacagc ggatcctttc ttccggactt atggttcgcc
      301 gtgtgaacat ccgccagctg atgccttttg aaggaacacg cgcatatgag gataacacgc
      361 ttggacaaca cgacagactg ttccaccagt ttaaagagcg tgttcgggaa acgtttgatg
      421 tgccgatgct tgcacgtgtg ttccctgtcg gtactgtttt gcgtgatatt gtcattgagg
      481 tgtcgggatc ggtgtcattt ggcagacaga tgggaacgta tccgattctc tgcggtattc
      541 caatgcagat tccggtaggt actgtgatgg atgctgcggt tgtttcctat ggtcagcggt
      601 cggtgactgc acttccggtc ccaattgaga tcaaccggct gaatgctgct gcgctgaaat
      661 ggctgccggg cgtatccaaa aaagctgcgg cacagattgc tgcaaaaaaa ccgtatgcgg
      721 atgcagctgc atttcgctca gcggtttcct cagatgctgt tgcccctctt attgagctga
      781 tgcggtttta aagcgtcttc cagagcacaa gccgaggagt agtcggataa aagtagtttt
      841 taatgcgttc cttttgcatg aatcctattt tttggtaaaa cgcaattgcc ggtttgtttg
      901 tctcagccac cgtcagatgg accgctgtaa catttcgctc tttcattgca gaaaacagtg
      961 cctcggtaag ttttgtaccg cattccttgc gctgatagtt ctgcacaacc cctattcgga
     1021 tcagttttcc ggttttcgga tcatcgagat gaatgccgcc aaggatgtag ccgatgatat
     1081 ctttctcagc gttttctgca acgaagaaga gagaggggaa cagttcctca atgtggcgga
     1141 acagtaccgg tccccccata cctgcaaaca gcggcgcatc cagttcgcag atggatggat
     1201 aatctgcaga ggaataaggg cggacacgga tcgattccat atattcacaa gtatatgtgg
     1261 tttggagatg ttgaacatat ctcatagctt gtcccgcgac atgcaaacat acttatgctt
     1321 gcgggagtat aattttcaaa agtatgatga agtggcagat ctcggatctc tttggcatga
     1381 gcgtctattc agacaaagct gttttccttg gcaaggttga agatgttgtc ttggatgtta
     1441 ctaacaaaaa aatcagtggt cttgcactga ccagtgtaaa ccagaatgta cttgatatca
     1501 agaactatca gggtgttgtc ataccttatc gtatcgtaaa agaggtagga gacataattc
     1561 ttgtccgtca tattcccgga gccttcaaag tatccgagcc gctctttggg gaataatctg
     1621 taaatagaat aatgaaggat cgcgagattt ttaccggcct tatcaccacc gttcctttta
     1681 ttttgcggct ggacggaaga tccttccacc acttatcccg caacaacgga tatgccaaac
     1741 cgtatgatcc ggtgttttcc agatgtatgg tacaaaccgc gcaggcgatg cttgagaaca
     1801 gcggccttgc accatccttt gcctatacat tttcggatga gatcagcctc tatgtcacgt
     1861 cccctgtttt tgactgccgg gttgagaaac ttgtctctgt ggctgctggt tttgcttctt
     1921 cggcgttcac attgttttcc agatcagatg ttccgctttc ctttgactgc cgcgtagttc
     1981 cgattgacga ggtccatctt gcggagtatc ttgcctggag acaggctgag gcttggcgaa
     2041 atcacattaa cggctatgcg cataagttgc tcatggacac gggactttct gcgactgctg
     2101 cacagagaaa actggacggt atgaagtcct cggatctgca tgaacttgct ttttcttttg
     2161 gggtgaacct tgctgaaaca cctgcatggc agcggcgcgg gacagcagtg tatcggtcag
     2221 ttgtggagaa ggagggctac aacccggttt cccaggagg
//
LOCUS       DALUAJ010000080         1771 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_14435,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000080 DALUAJ010000000
VERSION     DALUAJ010000080.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14435"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="O0X37_01735"
     CDS             complement(<1..625)
                     /locus_tag="O0X37_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HJK61683.1"
                     /translation="MGFIEDISTATDIIRSADAVTLVSHIDADGITSEAIAAQAISRL
                     GIPVTPVFVRQLEPMTMRHVPNDDTLKVFTDLGAGQQNLMEDAGLPTDRVLILDHHIN
                     QPAPGGTVYPQVNSQFYGSEYAKCSAAGVAYMIARKLDPANTDLAELAVVGNVGDMMA
                     RETCCLTGLARWIADDGVEAGRIRISKGLNCYGLSTRPLHLCLANSDD"
     gene            774..>1771
                     /locus_tag="O0X37_01740"
     CDS             774..>1771
                     /locus_tag="O0X37_01740"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HJK61684.1"
                     /translation="MRELSSFGLSGIISADEMRVIDKNADGWGISALQRMESAGTALA
                     SAVRDERPDRVTIFCGTGNNAGDGFCAARHLANETDVVVFCVGDPKTPEARTQLAALA
                     ACPVTITKTITPDLFTRDVIVDALLGTGARADLKEPYADLVTRMNTSDARIIACDVPT
                     PGCRADRIVAFHLAKTPGSEVYNIGIPLAAEVFCGEGDLLLIPKKPAGSHKGSGGTVL
                     IVGGGPYQGAPFLAGVAALRAGADIVRVASPAEGFAPDIIHDRLSGDHVCEAHRDHLI
                     SLAENADAVVCGPGLGTAAESLAVAAEVVEASEKAVVDADLLRQPLPHAAGETIYTP"
BASE COUNT          430 a          458 c          485 g          398 t
ORIGIN      
        1 gatcatcact gtttgcaaga cagaggtgca gaggacgtgt ggacaagccg taacaattga
       61 gacctttact tatgcggata cgtccggcct ccacaccgtc atcagcaatc caccgggcaa
      121 gtccggtaag acaacaggtc tcgcgtgcca tcatgtcgcc gacattgcca accacagcaa
      181 gttctgcaag atcggtgttt gccggatcca gtttgcgtgc aatcatgtag gcaacgcctg
      241 ctgcagaaca ttttgcatac tccgaaccat aaaactggga gtttacctga ggataaacgg
      301 ttcctccggg cgctggctga ttgatgtgat ggtcgaggat caggacacgg tcggtaggca
      361 gtccggcatc ttccataaga ttttgctgac cggccccaag atcagtgaag accttaagcg
      421 tatcgtcgtt tggcacatgc cgcatcgtca tcggttcaag ctgccggaca aaaacagggg
      481 ttacagggat gccgagacgg gagattgcct gagcagcaat tgcctcgctt gtgataccgt
      541 cggcatcaat gtgcgagaca agtgttactg cgtccgcact tcggatgatg tcagttgcgg
      601 tactgatgtc ctcaatgaaa cccattctta tctacttggt gttgcggcaa actatagata
      661 tggtttacca cagctttgcc gcgcgagagg acacgccagg aaaattttgt cactacatca
      721 gacgaggaga agatagttta tcttctggca ggcaaacgta tgtacgacga aaaatgcggg
      781 aactatcatc cttcggcctg tcaggaatta tctcagcaga tgagatgcgt gtcattgaca
      841 aaaatgcaga cggctggggg atatctgcac ttcagcgcat ggaaagtgcc ggtacggcac
      901 ttgcatccgc agtcagggat gaacgaccgg atcgtgttac tattttttgc gggacgggaa
      961 acaacgcagg cgacggattc tgtgctgcac gccatctggc aaacgaaaca gatgttgttg
     1021 tattctgtgt gggagatcca aaaacccccg aggcacgtac ccagcttgcc gcgcttgcag
     1081 catgtccggt gacgataaca aagacaataa ctccagacct gtttacacgc gatgtgatcg
     1141 tggacgcact tcttgggacc ggagcgcgtg ctgatcttaa agagccgtat gcggatctgg
     1201 taacccgcat gaacacatca gatgccagga ttattgcctg tgatgtccca acaccaggct
     1261 gcagggcaga tcgcatcgtg gcatttcatc ttgcaaaaac tcctggttct gaggtgtaca
     1321 acatcggaat tccacttgct gccgaggtgt tctgcggtga aggagacctt ctcctgatcc
     1381 cgaaaaaacc ggcaggatca cacaaaggat caggcggcac agttctcatc gtaggcggtg
     1441 gcccgtatca gggagcgcct ttccttgcag gagttgcagc tcttcgtgcc ggggctgata
     1501 tcgtccgcgt cgcctcccct gcggaaggtt ttgcaccgga cataattcac gatcggctct
     1561 ccggcgatca tgtctgtgaa gcacaccgcg atcacctcat ctctcttgca gaaaacgctg
     1621 acgctgtagt ctgcggaccg ggacttggaa ccgcggcaga aagtcttgct gttgcggcag
     1681 aagtagtgga agcatcagaa aaagcggtag tggatgcaga tcttctccgc cagccgcttc
     1741 cgcacgcagc aggcgagacc atctatacac c
//
LOCUS       DALUAJ010000081         3642 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_14640,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000081 DALUAJ010000000
VERSION     DALUAJ010000081.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14640"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="O0X37_01745"
     CDS             <1..1365
                     /locus_tag="O0X37_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HJK61685.1"
                     /translation="IVNIALPTISDSFDLTSSSVAWVSTIYLLVMAGCLLIIGKLSDV
                     VGFKKIFLIGFILFTVGSFTCGFLPDVTGQFSTLLASRVLQALGGAMMTVIAPAMLSY
                     YLPGDKRAKGMSLVVLFAAVGMALGPTLGGYLTEYLSWHWIFFINVPVGIFAIILGYA
                     AIPASPVAKTSLKGFDSIGAVLVFVGLAALLFAFSEGSSLGWTSWPVLASLALAVLGL
                     GGFLWRELRCGDPLLDLSLFQSRSFVVLNVVFALLFFTFAGTNYLLPFYLEHVHNYSV
                     SESGLIITAMSVGMMITGILAGLIYAKLKGKIRYLVMAGIALVAVGFFFLTHLTPVTG
                     LNVIISALALIGLGLGLTTTPLTTLIMSAAPASKQGMVSSLTGLERFAPMTIGVAVYN
                     SIFMHGIMAAVRNSGITEKPPGDIAAAVLSYGFDLAFVVSVILAVVLFVLCIFIREET
                     AEEK"
     gene            1326..2294
                     /locus_tag="O0X37_01750"
     CDS             1326..2294
                     /locus_tag="O0X37_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HJK61686.1"
                     /translation="MHLYPGGDGRREITTAVFFDEIFSILNAEYSLSIRGCFAVFKIC
                     LVLSVVLVLAAVSAGCISTPAELDNNTVLLVTVPPMVEMVSTVAGDGFTVISVVPEGM
                     SPHTYEPTPSDVSSFAAADMWFTLGEGFLPLEDQVAEALPDLPRVVTGTGIQAVAEAG
                     SEEGETDPHIWLSARNGILMTKAICDGLSAQYPDLAERFSAQADAFCSALSEVDARLA
                     DAAASMEPKAFLTTHGSFGYLAEDYNITQLVIAREGKEPAARELAEIVDSAKSAGVHI
                     IVTEPLSGERTAMVLSEELGIEPVQINPLSVHYMDTLNSLEEVLKG"
     gene            2291..3073
                     /locus_tag="O0X37_01755"
     CDS             2291..3073
                     /locus_tag="O0X37_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK61687.1"
                     /translation="MSEVLEIDHLVVERSGHVVLDDVSFVLHQGDVAAIIGPNGGGKT
                     SFLMSILGLIQPKSGTIRVFGEEPAAARARIGYVPQVHPFDFWYPMTVQEMVLTGRLG
                     HIPGLVKRYHEKDYAAAKDALAAMDLTGYENRPIAELSGGEQQRAVISRALAGCPQLL
                     ILDEPTVYVDGPTGERLMTLLEELRDRMTVLMVTHDISAMSGHVNRVACLNRKLFTHD
                     TDKITGDMVAAAYGCPVELITHGQVPHRVLARHDDTDQEGNQ"
     gene            3070..>3642
                     /locus_tag="O0X37_01760"
     CDS             3070..>3642
                     /locus_tag="O0X37_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HJK61688.1"
                     /translation="MTDLLSYVFMQHAFLAAALASVVCGIIGSYIIVRRMTAISGGIS
                     HATFGGVGLGYLLDFSPIAGAAGFAVSAAVVMGILKEYAHQKVDMLIGAVWAVGMALG
                     VLFVALTPGYVPDIESYLFGNILLVTSGDLILAGVFAVVITAVVALLYQPFLAVTFDE
                     EYATVMNLPVKMLNLLLLILIALAVVMLIQV"
BASE COUNT          726 a          841 c         1013 g         1062 t
ORIGIN      
        1 attgtcaaca ttgctctgcc aaccatttcc gactcgtttg atctcacgtc ctcttctgtt
       61 gcctgggttt caacgattta tctgctggtt atggcaggct gtttgttaat catcggaaag
      121 ttgtcggatg ttgttggatt caaaaagatc ttcctcatag gtttcatact gtttaccgtc
      181 ggttctttca cctgcggatt tttaccggat gttaccggac agttttccac tcttctggca
      241 tcccgagtac ttcaggcact gggcggggca atgatgacgg tgattgcgcc tgccatgctc
      301 tcttactatc tgccaggtga caaacgggca aaaggaatgt cgctcgttgt gttgttcgcc
      361 gcggtaggga tggcgcttgg ccctaccctt ggaggatatc tgactgagta tctgtcctgg
      421 cactggatct ttttcatcaa tgtgccggtt ggaatatttg ccataatatt aggatacgcg
      481 gctattcctg cgtctcccgt tgcaaaaaca tccctgaaag ggtttgacag tatcggggca
      541 gttcttgtgt ttgtcggact tgccgcactt ctcttcgcct tttccgaagg cagttccctt
      601 ggctggacct catggccagt tcttgcatcg cttgcccttg cagttctggg acttggagga
      661 tttctctggc gcgaactccg ctgtggagat ccccttcttg atttgagtct tttccagagc
      721 cgatcatttg ttgtgctgaa tgttgtgttt gccctgctct tcttcacgtt tgccggtaca
      781 aactatcttc ttcctttcta tctggaacat gttcacaact acagtgtttc agaatccggt
      841 ctgatcatca ccgcaatgtc ggtcgggatg atgatcacag gaattcttgc cggactgatt
      901 tatgcaaagc tgaaaggaaa gatccggtat ctcgtgatgg caggtattgc actggttgcg
      961 gttggattct ttttcctgac ccatcttact cctgtcaccg gccttaatgt gatcatctct
     1021 gctcttgctc tcattggcct tggtcttggt cttacgacca ctccactcac aaccctcatt
     1081 atgagtgcgg ctcccgcatc aaaacagggt atggtctcga gtcttacagg tcttgagcgt
     1141 tttgcgccga tgacaattgg tgttgctgtc tacaatagca tctttatgca cgggatcatg
     1201 gctgcagtcc gcaactcagg cattaccgaa aaacctccgg gagacattgc cgctgctgtt
     1261 ctcagttacg gctttgatct ggcatttgtg gtgtctgtca tactggctgt ggttcttttc
     1321 gtgttgtgca tctttatccg ggaggagacg gcagaagaga aataaccact gccgtctttt
     1381 ttgacgaaat ctttagcatc ctaaacgctg aatattcatt atccatacgg gggtgttttg
     1441 ccgttttcaa aatatgtctt gttctaagcg ttgttctggt acttgccgca gtttcggcgg
     1501 gatgtatctc cactcccgct gagttggata acaatacagt actgcttgtt accgttcctc
     1561 ctatggtgga gatggtctcg acggttgccg gagacgggtt caccgttatc tcggttgtgc
     1621 cggaggggat gtctcctcac acatatgaac caaccccttc cgacgtgtca tcttttgctg
     1681 ctgcggacat gtggtttaca cttggcgagg ggttcctgcc gctggaggat caggtggcag
     1741 aagcgctgcc ggatctgccg cgtgttgtaa caggtaccgg aattcaggcg gttgccgagg
     1801 ccggcagtga ggagggagag acggaccccc acatatggct ttccgcccgg aacgggattt
     1861 tgatgacaaa agcgatatgt gacggacttt cagcacagta tcccgatctt gcagaacgat
     1921 tttctgccca agctgatgcg ttttgttctg cgttgtctga ggtggatgcc cggcttgcag
     1981 atgcagctgc ctccatggaa ccaaaggcgt ttttgacgac tcatggatcg tttggctatc
     2041 tcgcggaaga ttataacatc acacaactgg ttattgcccg cgaaggaaag gagcctgcag
     2101 cccgtgaact tgcggaaatt gttgactcgg caaaatcagc aggtgttcat atcattgtga
     2161 ctgaaccttt gtcgggagag aggaccgcga tggtactttc cgaggagctt ggaattgagc
     2221 cggtacagat taacccgttg tcggtccatt atatggatac actgaattct cttgaggagg
     2281 tactgaaagg atgagcgagg ttttggagat tgatcatctg gtagtggaac ggtccgggca
     2341 tgtggtgctg gatgatgtga gttttgttct gcaccaaggt gatgttgcgg caattatcgg
     2401 accaaacggc gggggaaaaa catcattttt gatgagcatc cttggtctga ttcagccgaa
     2461 atcagggaca atccgtgtct ttggcgaaga acctgcagca gcacgggcac ggattgggta
     2521 tgttcctcaa gtacacccat ttgatttctg gtatcccatg acggtacagg agatggttct
     2581 gactggacgt ctcgggcata ttcctggatt agtaaaacgg taccacgaga aggattatgc
     2641 agcggcaaaa gatgcccttg cggcgatgga tctgaccggg tatgagaacc gcccgattgc
     2701 cgagctgtca ggaggcgagc agcagcgtgc tgtgatctcc cgtgctctgg ccggctgccc
     2761 ccagctcctg attcttgatg agccgacggt gtatgtggac ggacctaccg gggaacggct
     2821 gatgacgctt ttggaggaac tgcgtgatcg tatgacggtt ctgatggtaa cgcatgacat
     2881 tagtgcgatg tcgggacatg tgaaccgggt tgcgtgcctg aatcgaaaac tcttcacgca
     2941 tgatacagac aagattaccg gagacatggt ggcagccgcc tacggctgtc ccgttgagct
     3001 gattacgcat ggtcaggttc cccaccgtgt tctcgcaaga cacgatgata ctgaccagga
     3061 gggcaatcaa tgacggatct gctgtcctat gtgtttatgc agcatgcttt tcttgccgct
     3121 gcactcgcaa gcgttgtctg cgggattatc gggagttaca ttattgtccg gcgaatgact
     3181 gcaatctcag gaggaatctc tcatgcgacg tttggaggtg tgggtcttgg atacctgctt
     3241 gatttttccc cgattgccgg tgccgcaggt tttgctgttt cggcagcggt ggtgatgggg
     3301 attttgaagg agtatgcgca tcagaaagta gacatgctga ttggtgcggt ctgggcggtt
     3361 gggatggcac ttggagtact gtttgttgca ctgacgcctg gatatgttcc ggatattgag
     3421 tcgtatctgt ttggtaatat tctgcttgtg accagcggcg atcttattct tgccggtgtt
     3481 tttgcggttg tgattacagc ggttgttgca ctgttgtatc agccgttcct tgcggtgacg
     3541 tttgatgaag agtatgcaac ggtgatgaat ctaccggtga agatgctgaa tcttcttctg
     3601 ctgatcctga tcgcactggc ggtggtgatg ctgattcagg tg
//
LOCUS       DALUAJ010000082         3642 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_14765,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000082 DALUAJ010000000
VERSION     DALUAJ010000082.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14765"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1927)
                     /locus_tag="O0X37_01765"
     CDS             complement(<1..1927)
                     /locus_tag="O0X37_01765"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HJK61689.1"
                     /translation="MADSVVDSILAARYYRVGEKSFEDVCRRVANALGETPEETKEYF
                     EAMMSLEFLPNSPTLMNAGTPLGQLSACFTLPVNDSLPEIFDAIRWGAIIHQSGGGTG
                     YNFSHLRPEGSPVRSTDGVASGPVSFMRVFNAATDVIKQGGRRRGANMGILNVWHKDI
                     MKFIKSKAKEGDFSNFNISVMVNDEFMKAVASGDLDREWLKTTDGETVTVREIWDGIV
                     EGVWKNGEPGILFYDTINRKNPTPQLGDIDTTNPCGEQPLLPFESCVLGSINLAKFIR
                     ADGVNYDALDKMTRMAVRFLDAVITKNVFPIEQIKEATNRTRKVGLGLMGVHDAMLML
                     RIPYDSEAGRNFCEEVMARINDVATEESERLGKEKGTFPAYKGSVWDKQGKIMRNAAL
                     TTIAPTGTISLLAGCSSGIEPVFSYAYTRRNTVGKTFIMVHPYFESELRRVIAGLGFT
                     GKEAEAKRDEVISHVHETGTVQDVLWLPDAFRAVFKTALDIQWKDHVLMQAVFQKHVQ
                     ASISKTINMPFSATKEQVADAVLLAWKEGIKGMTLYRTGSREDVVLALEKKEEKKEEP
                     VAAAEPKVVQQLPFSRPRELDGRTFLAQSGCCRLYITVNTMNGKPMEVFIRTVGAGCE
                     ASSNALGRSISTGLQNGV"
     gene            2111..2662
                     /locus_tag="O0X37_01770"
     CDS             2111..2662
                     /locus_tag="O0X37_01770"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HJK61690.1"
                     /translation="MVVPEKVFFTRGCGVHKDKLVSFEMALRDAGLAPYNLVTVSSIM
                     PPDAEIISREEGLPYLSPGEIVYCVMARTETNIPGQAVAASVGLAVPEIHGKQHGYLS
                     EYHAADISGHECGEYAEDLAATMLATIMDIPFDPETAWQEREQVYLASGKIIKTSHLA
                     ASAVCGDDGKWTTVIVAAVFILP"
     gene            2727..>3642
                     /locus_tag="O0X37_01775"
     CDS             2727..>3642
                     /locus_tag="O0X37_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HJK61691.1"
                     /translation="MCGIAGIVANSEVSGSLYWALYALQHRGQESGGISTYDNGIVHK
                     YKGYGLVSEIFTSSILEKLSGKTGLGHVRYPTTGGSKPENIQPFNFLFRGHSLSIVHN
                     GNLVNTDELRYEYEGRGHIFSTTSDTEVIAAILANEIIHGHTVVEAISFCMRSIRGSY
                     AVIFMLDGVLYAFRDPLGIKPLCLGEFEDGGHIVASESVALDAVGAKLIRDVAPGESI
                     RLMADKVYPHQIMTAKSPAHCVFEYIYFARADSVIDGISVYKVRQKTGFLLAGESPAH
                     ADIVSPVPDSGTAAATGYAAASGIPFREA"
BASE COUNT          796 a          977 c          923 g          946 t
ORIGIN      
        1 gtactccgtt ctgcaagcca gtactgatac ttctgccaag tgcattgctg cttgcttcgc
       61 atcctgcacc gactgtccgg atgaacacct ccatcggttt tccgttcatc gtgtttaccg
      121 taatgtagag acggcagcat cccgactggg caaggaatgt tcttccgtcc aactcacggg
      181 gacgtgagaa cggcagctgc tgtacaacct tcggttctgc cgctgctacg ggctcctctt
      241 ttttctcctc cttcttctca agtgccagta ccacgtcctc ccgtgagccg gtgcggtaca
      301 gcgtcatgcc tttgatgcct tccttccagg cgagcagaac tgcatcggcc acctgttcct
      361 ttgttgcaga gaacggcatg ttgatcgttt ttgagatgga tgcctgcacg tgcttctgga
      421 atacagcctg catcagcaca tgatccttcc actggatatc aagtgcagtc ttgaacacag
      481 cacggaatgc gtccggaagc cacagtacat cctgaacggt tcctgtctca tgcacatggc
      541 tgataacttc gtcccgcttt gcctctgctt ctttcccggt aaatccaagg cctgcaatta
      601 cgcgccgcag ctcggactcg aagtatgggt gaaccataat aaacgttttt ccaacggtgt
      661 tccggcgggt gtaggcgtac gaaaacaccg gttcaattcc tgatgaacat cctgcaagca
      721 gtgagatcgt tccggtcggg gcaatagtcg taagtgccgc gttgcgcata atctttccct
      781 gtttgtccca gacggatcct ttgtatgcgg ggaatgttcc tttctccttt ccaagccgct
      841 cggactcttc cgttgccaca tcgttgatac gtgccatgac ctcttcgcag aagttgcgcc
      901 cagcctccga gtcgtaggga atgcggagca tcagcatggc atcgtgtact cccatcaggc
      961 caagaccgac ttttctggta cggtttgttg cctccttgat ctgctcgatc gggaatacgt
     1021 tctttgtgat gaccgcatcc aggaaccgca ccgccatccg cgtcatctta tccagtgcat
     1081 cgtagtttac cccatcggca cggatgaatt ttgcgaggtt gatacttccc agaacacagg
     1141 actcaaacgg cagcagcggc tgttctccgc acgggttggt cgtgtcgata tcacccagct
     1201 gcggtgtcgg gtttttccgg tttattgtat cgtagaaaag aattcccggt tccccgttct
     1261 tccatacacc ttccacaata ccgtcccaga tctcgcgaac agtcaccgtc tcaccgtcgg
     1321 ttgttttcag ccactcgcga tccagatcgc ctgatgcaac ggctttcatg aactcatcgt
     1381 ttaccatcac agagatgttg aagttcgaaa agtctccctc ctttgctttg cttttgatga
     1441 acttcatgat atccttgtgc cagacgttca ggatacccat gtttgcgccg cgcctgcgcc
     1501 ctccctgttt gataacatct gttgccgcgt taaagacacg cataaacgac acagggccgg
     1561 atgcaactcc gtctgttgag cggacagggg atccctctgg tctgaggtgg gagaagttgt
     1621 agccggtacc gccgcctgac tgatggatga ttgcacccca gcgaattgca tcaaaaatct
     1681 ccggcagaga gtcgtttact gggagtgtaa aacaggcgga cagctgtccg agcggggttc
     1741 ccgcattcat cagtgttggt gagttcggga gaaattcaag gctcatcatc gcctcaaaat
     1801 attctttcgt ctcttccgga gtttctccga gggcatttgc cacacgcctg caaacatcct
     1861 cgaatgattt ctcaccgacg cggtaatagc gtgcggcaag aatgctgtct acaactgagt
     1921 ccgccataaa atcaacctga tattattagt acaattttca ttttgccgga ggaaatgggg
     1981 atagtccata cggccgggtg cagagtactt accaagtaat tagcgagcac gattattaat
     2041 ttttgacatt tggcataagg ggggtaaatc acaactatta atgagatgca catcaatttt
     2101 ttaaagaatc atggttgttc ccgaaaaggt attttttacc agaggatgcg gagtccacaa
     2161 agacaagctg gtgtcctttg aaatggccct cagggatgcg ggacttgccc cgtacaacct
     2221 cgtaacggtc tcctcgatca tgccgccgga cgctgagatc atctcccgcg aggagggact
     2281 gccttatcta tcgccgggtg aaattgtcta ctgcgtcatg gcgcggacgg aaacaaatat
     2341 tccggggcag gctgttgccg catccgttgg tcttgcggta ccggagattc atgggaaaca
     2401 gcatgggtat ctctccgagt atcatgccgc agatatctcc ggtcatgagt gcggtgagta
     2461 tgctgaggat cttgcggcaa caatgcttgc caccattatg gacatcccgt tcgatcctga
     2521 aactgcgtgg caggaacgcg agcaggtcta tcttgcaagc ggaaagatca tcaaaaccag
     2581 ccatcttgcc gcatccgcgg tatgtggtga tgacggtaag tggaccaccg ttattgttgc
     2641 cgctgtcttc attcttccat aatattcttt tttttctgcg ggacaggcga gtaactaatt
     2701 accttccagc gctaatactt ctccacatgt gcggtatcgc gggcatcgtc gcaaactccg
     2761 aggtttcggg ttcattgtac tgggcgctgt atgcacttca gcaccgcgga caggaaagcg
     2821 gtggaatatc cacctatgac aacggaatag ttcacaaata caaaggatac gggctggtgt
     2881 ccgagatatt cacgagcagc attctggaga agctttccgg gaaaacagga cttggccacg
     2941 tccggtatcc gacaaccggc gggtccaaac cggagaatat ccagccgttt aactttttgt
     3001 tccgcggtca ttcgctatcc atcgtccaca atggaaatct ggtcaacacc gatgagctgc
     3061 gttatgagta tgagggcagg ggtcacatat tttccaccac ctccgatact gaagtgatcg
     3121 ccgcaatttt ggcaaacgag atcattcacg ggcacaccgt tgttgaagcc atcagcttct
     3181 gcatgcggag tatcagagga tcatatgcgg tcatcttcat gctggacggc gttttgtatg
     3241 cgttccgcga tcccctcggc atcaaacctc tctgtcttgg ggagtttgaa gacggaggac
     3301 acatcgttgc atccgaaagt gttgctttgg atgcagtcgg tgccaaactg attcgcgacg
     3361 ttgctcccgg ggaaagcatc cgtctcatgg cagacaaggt gtatcctcat cagatcatga
     3421 ccgcaaaatc tccggcgcac tgtgtgtttg agtacatcta ttttgcccgg gccgattcgg
     3481 tgatcgatgg tatctctgtc tacaaggttc gtcagaagac tggattcctt cttgcaggtg
     3541 agtcacctgc gcatgctgac attgtttcac cggtgccgga ctcaggaact gccgctgcca
     3601 cagggtatgc tgcggcgtcg ggcattccct tccgagaagc ac
//
LOCUS       DALUAJ010000083         2456 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_14782,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000083 DALUAJ010000000
VERSION     DALUAJ010000083.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14782"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..963)
                     /locus_tag="O0X37_01780"
     CDS             complement(<1..963)
                     /locus_tag="O0X37_01780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK61692.1"
                     /translation="MLIERPLSQKLLMILAAAAVFMDYLDTSIVSIALPAISESFGSG
                     SSLSSWVMTSYLLALGSTLLLFGKLADRTGLQRIIFTGGFVLFTLASFFCGIATDIVS
                     LIGFRVFQGVAAAMMVATATMLITLHLPKQIQPIGMGVIATAGGVALALGPGIGGVLT
                     QYISWHWIFFINIPVGIVGVLGALFLIPKPDPADLPTRSKPFDSLGAVYLAATLVTLL
                     AGLEFGVSDGWTLPVLLLIVLSPVFGYLFLHRELRHPDPILSARLLYNRTVMWAAVST
                     LCITLVYLGVIYVMPFLLTEEFLLPTALAGSIMLLPPVAMALVGI"
     gene            complement(1041..1490)
                     /locus_tag="O0X37_01785"
     CDS             complement(1041..1490)
                     /locus_tag="O0X37_01785"
                     /inference="COORDINATES: protein motif:HMM:NF020489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61693.1"
                     /translation="MRLLLIHADLIAYETLSKTKMAEPEIVAKDEMQDALVVFCAVES
                     VDETDLDDIAAQACAEIASVATKVGATRVMIYPYAHLSSDLASPSSAVNILKEIVRVL
                     ADSTSLEVRRAPFGYYKKFTVTCKGHPLSELSRHLVPSSNHKGQSLQ"
     gene            complement(1605..>2456)
                     /locus_tag="O0X37_01790"
     CDS             complement(1605..>2456)
                     /locus_tag="O0X37_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HJK61694.1"
                     /translation="SYAGKYLLRIAIILMGARVTADVLMSASASGLVIILAVLAVTIV
                     LAMMLGKKFGHNWETSALTGTGNGICGVSATLSVAPVIKADQKYVHAVVGVISLLGIV
                     GVFLIPAIAVGVGMNDAQAEVFIGGTLHEIGNVIPAADLYTAISGGEDVGALSLAYKM
                     IRVAMLVVVASVFGWLWCRRQEMEQASCPADRVKAKVQGFLILFVIMAVGMTAFITAS
                     PDLGHAVQSSLVNISVTILTVAMAGVGLSMNLRETLSIGKTLLPFASLIWLIQVVLLL
                     GLTMFLV"
BASE COUNT          743 a          620 c          609 g          484 t
ORIGIN      
        1 aattcctacc agtgccattg caacaggagg cagaagcata atactgcctg ctagtgcagt
       61 cggcaaaaga aactcttccg taagaaggaa cggcatcacg tagattacac caaggtagac
      121 aagggtgatg cacagcgttg aaaccgcagc ccacatgacc gtacggttgt aaagcagacg
      181 ggccgagaga atcggatcgg gatgccggag ctcgcggtgc aaaaacaagt aaccgaaaac
      241 cggtgacagc acaataagca ggagaactgg cagtgtccag ccatcggata ccccgaactc
      301 cagccctgca aggagcgtaa ccagcgtagc cgcaagatat accgctccaa gcgaatcaaa
      361 cggtttggat ctcgtaggta gatcggcggg atcgggtttt ggaatcagga acagcgcacc
      421 taaaacaccc actataccca cggggatgtt aatgaaaaat atccagtgcc agctgatgta
      481 ctgggtaagt actccgccga ttcccggacc aagggcaagt gccaccccgc cggcggtggc
      541 gatgacgccc atccctattg gctggatctg tttggggaga tgcaacgtga tcagcatcgt
      601 agctgttgca accatcatgg cggcagcaac cccttggaag actcggaacc ctatcagcga
      661 gacaatgtct gttgcaatgc cgcagaagaa tgaggcaaga gtaaacaata caaatcctcc
      721 ggtgaagata atccgctgaa ggccggtccg atcagcgagc ttgccgaaga gaagaagcgt
      781 acttcccagt gcgagcagat acgaggtcat cacccatgac gacagggacg aaccggaacc
      841 aaaactctca gagattgcag gcagtgcaat ggagacgatg cttgtatcaa ggtaatccat
      901 aaagacagcc gcagctgcca gaatcatcag aagtttttgg gagagaggac gttcgatcag
      961 catcaacaca caccggatag gggatgaaaa acagagcgga gtactggatg tatcccaccc
     1021 tgttatcgga agatatggat ctattgaagg gactgaccct tatggttgct ggatggaaca
     1081 agatgccggg aaagttctga gagaggatga cctttgcagg taaccgtgaa ctttttgtaa
     1141 tacccaaacg gtgcacgccg cacttccagc gaagtactgt ccgctaaaac acggacaatc
     1201 tctttgagga tattgactgc tgaagaaggc gaggcaagat cacttgagag atgagcgtac
     1261 ggatagatca tgacacgggt tgcaccaact ttggtagcga ccgatgcaat ctccgcacat
     1321 gcctgtgcgg caatatcatc caggtctgtt tcatctacgg actcaacagc acagaacacc
     1381 acaagagcgt cctgcatctc gtcctttgca acaatctccg gctctgccat ctttgtttta
     1441 gagagagttt catacgcaat aagatcagcg tgaatgagaa ggagacgcat actacatcca
     1501 ggtaactctg tatttcacca tacttaggtg aattatgtgt acatgggcgt taaacccata
     1561 ttaaggtaaa gtaagaagca gtacctggaa aaagagagtc tggattatac caagaacatg
     1621 gtcagcccga gaaggagcac aacctgaata agccagataa gtgaggcaaa cggaagcagt
     1681 gtcttgccta tagaaagtgt ttcacgcaga ttcatcgaga gaccaacccc ggccatcgca
     1741 acggtcagaa ttgttactga aatgttgaca agagatgatt gtactgcatg cccaagatcc
     1801 ggtgacgccg tgatgaacgc agtcatcccg actgccataa tcacaaagag gatcaaaaat
     1861 ccctgaacct ttgccttcac acgatcagct ggacaggaag cctgttccat ctcctgacgt
     1921 ctgcaccaca gccagccgaa caccgatgcc accacaacca gcatagcgac acgtatcatt
     1981 ttgtaagcga gagaaagtgc accgacatcc tctcctccgg agatggcagt gtaaagatcc
     2041 gcagcgggaa taacattgcc aatctcatgc agtgttccgc cgatgaacac ctcagcctgt
     2101 gcatcattca tacctacacc aactgcgatt gcaggaataa gaaacacacc aacaattcca
     2161 agaagactga taaccccgac aacagcatga acgtacttct gatctgcttt gatgaccgga
     2221 gcaacagaca gggttgcaga gacaccacag ataccattac ctgtacctgt cagtgcagag
     2281 gtctcccagt tgtgaccaaa ttttttccca agcatcattg cgagaacaat ggtaaccgca
     2341 aggactgcaa gaataatgac aagtccgctt gcagacgcgc tcattaacac atccgctgtg
     2401 acacgtgcac ccatcagaat aatggcaatg cgcagaagat attttccggc atacga
//
LOCUS       DALUAJ010000084         4475 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_14849,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000084 DALUAJ010000000
VERSION     DALUAJ010000084.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14849"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="O0X37_01795"
     CDS             complement(<1..204)
                     /locus_tag="O0X37_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HJK61695.1"
                     /translation="MVGDSELSRIGISLPQNLLDKFDGIIGLRGYSSRSEGIRDAIRS
                     YITYYEWMSDVKGERQGVITMVYD"
     gene            380..1615
                     /locus_tag="O0X37_01800"
     CDS             380..1615
                     /locus_tag="O0X37_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     gamma"
                     /protein_id="HJK61696.1"
                     /translation="MHDPTIPNVNIGVVGHVDHGKTTLVSQLTGSWTDRHSEELKRGI
                     SIRLGYADATFYKCPKCEGADSWSNADTCPICGEKLEPVRSVSFVDAPGHETLMATML
                     SGSAIMDGAMLVIAANEPCPQPQTKEHLMALELTGIKNIVIVQNKIDVVPQKQAIENY
                     KQIKAFVKGTVAENAPIIPVSAQKKINFGVLIDALNTTIPDTERDADVPPIMLIARSF
                     DVNRPGSSWREIKGGVIGGSLIRGVFREGDDIEIRPGRQYMSENKAKWEPITTKITSM
                     NKASHKVPVATPGGLSAIGTKLDPAITKSDTLVGQVAGAVGSLPPVWDKLKFDMTLMD
                     RVVGSASEQRIEPLRLKEPLMLSVGTAVTVGIVMAAKKNQAEVILKRPVCAELGSRIA
                     ISRQVGGRWRLIGMGILTE"
     gene            1579..1992
                     /locus_tag="O0X37_01805"
     CDS             1579..1992
                     /locus_tag="O0X37_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HJK61697.1"
                     /translation="MASDRHGHSDRVNVILDTNALMMPAQFGVDLFEGLRELLGGYDA
                     LVPAEVVYELRGLAQGRGNNAAAARFGLTVAARCTVLPPLEVEVPVDDKVIRTAEMFN
                     AVVVTNDKKLKDRLLSLRIPVVVLRSRSRLELMGK"
     gene            2013..2585
                     /locus_tag="O0X37_01810"
     CDS             2013..2585
                     /locus_tag="O0X37_01810"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HJK61698.1"
                     /translation="MYYKLKLNDKVRVPPERMGEDLNTVILDVLQEQFEGSVDKEMGI
                     FIAVTGVDRVGDGEIIYSDGGVYYDVDFEAVVLRLTLQEIVEGIVVETTSFGAFISLG
                     PIDAMLHMSQITDEYIDYDEKNSRLVCKDTGRTIAVGDRVRARVVALSLNEREPRESK
                     IGLTMRQPGLGTLAWIEDERNREKAEKERK"
     gene            2585..2800
                     /locus_tag="O0X37_01815"
     CDS             2585..2800
                     /locus_tag="O0X37_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase, subunit E''"
                     /protein_id="HJK61699.1"
                     /translation="MAPKRTQKKLLQACRTCHKVLEADKTVCPECQGNALTTEWFGYL
                     VIIDSRHSDVAKKMNIEYNGRYALKVR"
     gene            2800..3285
                     /locus_tag="O0X37_01820"
     CDS             2800..3285
                     /locus_tag="O0X37_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-dependent dephospho-CoA kinase family
                     protein"
                     /protein_id="HJK61700.1"
                     /translation="MLVLPSEHRGLFKQPFGTLFQEFSDIVPQISGRIVCTVGDVVTH
                     SALTRDITPAIGVIDGFTMRSPYLAMPEIAQHVLRVKNPAGTITEELVAVLDEAVRSM
                     PCMILVDGEEDLAVLPLIERLPDGALILYGQPNEGVVVCEVTPVLRQHAKELLSYFES
                     V"
     gene            3415..3708
                     /locus_tag="O0X37_01825"
     CDS             3415..3708
                     /locus_tag="O0X37_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S24e"
                     /protein_id="HJK61701.1"
                     /translation="MEIKITSNTRNELLSRNEVAFTATYDGATPARADIGAKIAAMQN
                     TPVENLILSPLKGRFGARAVTGVARIYDTPEALKATEREYLIARGQPKAAEEE"
     gene            3713..3895
                     /locus_tag="O0X37_01830"
     CDS             3713..3895
                     /locus_tag="O0X37_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27ae"
                     /protein_id="HJK61702.1"
                     /translation="MAAKKAAKEPQKRSALYSVDGQGKATTTHRHCPRCGPGVFMGEH
                     KDRFACGKCGYTEFKQ"
     gene            3907..>4475
                     /locus_tag="O0X37_01835"
     CDS             3907..>4475
                     /locus_tag="O0X37_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HJK61703.1"
                     /translation="MPETRVLGIEGTAWNFSAAVFDEDLVCLHSSPYVPPHGGIHPRE
                     AAQHHAAVAAEVIGKVLAEAGDDIDGVAFSIGPGLGPSLRTVATAARSLALKYDVPLI
                     GVNHCVAHVEIGRWYTKFSDPIVLYASGANTQVLGFLNGRYRIFGETLDIGLGNALDK
                     FARSHDLPHPGGPIIEEMAKKGSYIPLPYT"
BASE COUNT         1141 a         1065 c         1238 g         1031 t
ORIGIN      
        1 atcatagacc atggtaatca ctccctgccg ttcccccttg acatcggaca tccactcata
       61 ataggtgatg taactgcgga tggcgtcgcg aataccctct gagcgggaag agtagccgcg
      121 aagtccgata attccgtcaa atttatccag aagattctgc ggaagagata tgccgatgcg
      181 tgaaagctcc gagtctccta ccataatata gtattatatc aggctgttcc tttattactc
      241 ttattacata atcttatgca gagcgagagt ttgtcatacg aagtctgttg tccgtttgca
      301 aagaagacgg gagaggacta ccacacaaat atatctgttt ggaggtcgat tatatagggt
      361 tgaggaggtt ttggtttctt tgcatgatcc tacaatacca aacgtgaata tcggcgtggt
      421 aggccacgtt gaccacggaa agaccaccct ggtcagtcag cttaccggct catggaccga
      481 ccgccacagt gaggaactga agcgcgggat ctccatccgc ctcggatatg ctgatgcgac
      541 gttttacaag tgccccaaat gtgagggagc agattcctgg tctaatgcag acacctgtcc
      601 gatctgcgga gaaaaactcg aaccggtgag atcagtttcc tttgtggatg ctcccggaca
      661 tgaaacgctg atggcaacaa tgctctcggg ctctgccatc atggacggag caatgctggt
      721 cattgccgca aatgagccgt gcccgcaacc gcagaccaaa gaacacctga tggccctgga
      781 gctgaccggt atcaaaaaca tcgtcattgt gcagaataag attgatgtcg taccccagaa
      841 acaggcaatt gaaaactaca aacagatcaa ggcattcgtg aagggaacag ttgcagaaaa
      901 tgcgccaatc attccggttt ccgcacagaa aaagatcaac ttcggagtct tgattgatgc
      961 gctgaacacc accattccag acacagagcg ggatgctgat gttccgccga ttatgctgat
     1021 cgcccgatcc tttgatgtaa acagaccagg gagttcctgg agagagatca agggaggagt
     1081 tattggtgga tctttaatcc gcggggtctt ccgtgagggg gatgatattg agatccgtcc
     1141 cggaagacag tacatgtctg agaacaaggc aaaatgggag ccgattacaa ccaaaattac
     1201 ctccatgaac aaggcatccc acaaagttcc ggttgcaacg ccgggaggcc tgtctgccat
     1261 tggtacaaag cttgacccgg cgatcacaaa gagtgataca ctggtgggtc aggttgccgg
     1321 agcggtaggt tctcttccgc cggtctggga caaactgaag tttgacatga ccttaatgga
     1381 tcgcgtcgta ggatccgcat cagaacagcg tattgagccg ctgcggttga aagagccact
     1441 aatgctgtca gttggaacag cggttacagt tggtattgtc atggcggcaa agaaaaacca
     1501 ggcagaggtt atcctcaagc ggcctgtttg tgcggagctt ggatcccgca ttgcaatcag
     1561 ccggcaggtc ggtggcagat ggcgtctgat cggcatgggc attctgaccg agtaaacgtc
     1621 attctggata cgaacgcgct gatgatgcca gctcagttcg gtgtggatct gtttgagggt
     1681 ctgcgcgagc ttcttggtgg ctacgacgca ctcgtgcctg ctgaggtggt gtacgagctg
     1741 cgcggtcttg cacagggacg tgggaacaat gcggcggcag cacggtttgg tctaactgtt
     1801 gcggcgcgct gcacagttct tcccccattg gaagtggagg tccctgtgga tgacaaagtg
     1861 atccgaaccg cagagatgtt taatgctgtt gtagtgacca atgataaaaa gctgaaagat
     1921 cgcctgctca gtctccgcat tccggttgtt gtactccggt cacgatcacg acttgagttg
     1981 atgggaaaat aattcgacga ggagccaagg acatgtatta taaattaaaa ttaaacgata
     2041 aagtcagagt cccaccagaa cgtatgggtg aggatctgaa cacagtaatt cttgatgtac
     2101 tgcaggagca gtttgagggt agtgttgaca aagagatggg tatcttcatc gccgtcacgg
     2161 gtgttgaccg tgttggtgac ggtgagatca tctacagcga tggcggcgtg tactatgacg
     2221 tggactttga agccgttgta cttcgcctga cacttcagga gatcgttgag ggtattgtgg
     2281 tagagacgac aagctttggc gcgttcatct cgcttggccc cattgatgca atgctgcaca
     2341 tgagtcagat cactgatgag tacatcgatt acgatgaaaa gaactcacgt cttgtctgca
     2401 aagacacagg aagaacaatt gcggttggcg atagagtgcg cgcccgtgta gtggctctct
     2461 ccctgaacga acgtgagccg cgtgagtcaa agatcggact taccatgcgc cagcctggtc
     2521 ttggaaccct tgcatggatt gaggatgagc gcaaccgcga aaaggcggag aaggagcgga
     2581 agtaatggca ccgaagcgga cacagaagaa actcctgcag gcatgccgta cctgccataa
     2641 ggtcctggaa gccgataaaa cggtctgccc ggagtgtcag ggtaatgctc ttaccacaga
     2701 atggtttggg tatctggtca ttattgactc ccgtcactcg gatgttgcaa agaagatgaa
     2761 catcgaatac aacggccgtt acgcgcttaa ggttcgataa tgcttgttct cccgtcggaa
     2821 caccgagggc tgttcaaaca accctttggt accctttttc aagaattttc tgatattgtc
     2881 ccgcagatat ccggacgaat cgtttgtacg gtgggagatg tggtaacaca ctcagccctc
     2941 acacgagaca ttactcccgc gataggtgtt attgacggat ttaccatgcg ctctccctat
     3001 cttgcaatgc cggagattgc gcagcatgta ctgcgggtca aaaacccggc gggaacaatt
     3061 accgaggagt tggttgctgt ccttgacgaa gcggtgagat ccatgccgtg catgattctg
     3121 gtggacggtg aggaggatct cgcggttctg ccgctgatag aacgtctgcc cgatggtgca
     3181 ctgattcttt acggccagcc gaatgaaggg gttgtggtct gtgaggtaac acccgtactg
     3241 cgacagcatg cgaaggaact gttgtcctat tttgagtctg tgtgagtaaa tcctttgaga
     3301 aaatagggcg ggaagtagtc aaacatctgg gaaacagaag acttccgcac gaagaggttt
     3361 tcgaagataa caccagtatt aaatatactg cattccaata attgtgggat atcgatggag
     3421 attaagatca cctctaatac cagaaacgag cttttaagcc gtaacgaggt tgcattcacc
     3481 gccacatatg acggcgcaac ccctgcccgt gcagatatcg gagcaaaaat cgcagccatg
     3541 cagaacaccc cggtggaaaa cctcatcctc tctccgctga aaggacggtt cggagcaaga
     3601 gcagtcaccg gtgttgcacg catttacgat acgccggaag cactgaaagc aactgagcgc
     3661 gagtacctga ttgcccgcgg ccagccgaaa gcagctgagg aggagtaaga ttatggcagc
     3721 aaagaaggca gcaaaagagc cgcagaagcg cagcgcgctc tactccgttg acggacaggg
     3781 aaaagcaacc accacgcacc gccactgccc acgttgcggc ccgggtgtat tcatgggcga
     3841 gcacaaggac cgtttcgcct gcggaaagtg cggatatact gagtttaagc aataaaatac
     3901 attattatgc ccgaaacaag ggttcttggc attgaaggga cagcttggaa cttcagtgcc
     3961 gctgtttttg atgaggatct ggtttgtctt cactcctcac catatgtgcc tccgcatgga
     4021 ggaattcatc cacgcgaagc tgcccagcac catgcagcag ttgccgctga ggtgatagga
     4081 aaggttcttg cagaagccgg agatgacatc gacggtgtgg cattttccat cggaccgggt
     4141 cttggtccgt cactccgaac ggttgcaacc gcggcgcgtt ctcttgccct gaagtacgat
     4201 gtaccgttaa tcggtgtgaa tcattgtgtc gcacatgtgg agatcggtcg ttggtacacg
     4261 aaattctctg atcctattgt gttgtatgcg tcaggagcaa acacgcaggt actcggcttc
     4321 ctgaacggcc gttaccgtat tttcggtgag acgctggaca tcggccttgg gaacgcactg
     4381 gataagtttg cccgcagcca tgatctcccg cacccaggag gcccaatcat tgaagagatg
     4441 gcaaagaaag ggtcctacat tccgcttccc tacac
//
LOCUS       DALUAJ010000085         3986 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_15195,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000085 DALUAJ010000000
VERSION     DALUAJ010000085.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15195"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..2009)
                     /locus_tag="O0X37_01840"
     CDS             complement(<1..2009)
                     /locus_tag="O0X37_01840"
                     /inference="COORDINATES: protein motif:HMM:NF016219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF460 domain-containing protein"
                     /protein_id="HJK61704.1"
                     /translation="MSSLIFGIDIIRGSVRSGTIRPHYALVRVEDGEVISEEKNVTRF
                     RLMRYIRTELPEILAVDSIQEIAQGTADLYTFLAELPPGTKLVQVTGDGIKMETLPRV
                     AARYNLSFDKQNPMEEAKASALLASFGAGYEVLAYTGITDITVSRCRSPGRGGWSQNR
                     YIRKMHGAVRTHARDIEMKLISANLEYSMTVRRAFGGESRAVFTVYAPREQIPISPSK
                     GGDVQIRIAGRRRDKIAFQPLTKKPRYLIVGIDPGTTIGIAALDLDGTLIYLASTRLF
                     SAADLTREIATLGKPLIIASDKAEMPVGVEKIRRAFSAIAWNPKKDILIKEKYALAAG
                     YDFKNDHERDSLSAALYAYRTYKNKFESILKRAPAGVDTDELRAQIARGLSLEQALSQ
                     SSGTIPTTTETQKETDTVTEIIFDERDERITRQEETIRRLRALVETLSRESTGKDKTI
                     SSLHKKLNAEREMQHTELLARSEIIERDREIAAVKKQLRKEERRCKNLRVRLDRMKRY
                     VALQAGEGCSALKVLPQLSRDAVRSLDDEMGFGEEDLLYVLQIDGWGRSVVRELANAR
                     IKAVILPRHVYTRAQEQHLIEEFREAGLPVLSGADLSPRVKGQIGVADTAAVEEALAA
                     WDASQTVYSKEKKAAVINSTVKEYQAERVREVRELGIDPATIIPER"
     gene            complement(2006..2875)
                     /locus_tag="O0X37_01845"
     CDS             complement(2006..2875)
                     /locus_tag="O0X37_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HJK61705.1"
                     /translation="MPLSAEDIKNLHKYEVRILRALERMMHRYAWVPEDDLKAAVHLS
                     AAETKYRVGNLIHRDMVRSDAVPYKGYALVFKGYDALALSALAQKKTISALGTMIGVG
                     KESEIYEALGFGVVVLKLHKIGQRSFQTVRTNREYMPEKTHCPWIFASAKSAEREYEA
                     LKALNGKVNVPVPIDMNRHVIVMSFIPGVNLHRCRFEDPDKIWQEILSQVKSAYDAGF
                     IHGDLSEYNIMYDDTVVWIIDWPQWIPPDHVNADAVMRHDIETVAAFFAKKYQREYDI
                     DDAIRYVTGREVK"
     gene            2969..3562
                     /locus_tag="O0X37_01850"
     CDS             2969..3562
                     /locus_tag="O0X37_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61706.1"
                     /translation="MQCSRCGKEAVIVQPYSGVSLCAVHAARDLEAKAKRRIRKCGSI
                     SSGERLYVQESFGPESFALSVLLSRILAGRRDVSLVSDPSDATAVVSSETLDSSACCF
                     LQQIISGTTPEILSLPSRKVLDPLAVVPRVEVLQYARHHGWDGVDVRPRLSDPFTADI
                     CAFLEHFSVDHPSAAYALMQVRDALPQLYMERTNHAL"
     gene            3552..>3986
                     /gene="nadE"
                     /locus_tag="O0X37_01855"
     CDS             3552..>3986
                     /gene="nadE"
                     /locus_tag="O0X37_01855"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HJK61707.1"
                     /translation="MLCKDIGCAITRIKDLIRQTLWASGAKGVVVGISGGIDSAVATA
                     IAVKALGPEHVLGVHMPSASSSPLDIADAKDFCDKFGIELLTVPLGDVVSAAFSDPYL
                     MDTPILRGNYTSRLRMATLYNIAAARGFLVCGTSNKTEYMIGY"
BASE COUNT          822 a         1033 c         1035 g         1096 t
ORIGIN      
        1 cgctcgggga taatcgtggc aggatcaatt cctaactccc gcacctcccg tactctctcc
       61 gcctggtact cttttaccgt ggagttgatg acggcagctt tcttttcttt ggagtacacc
      121 gtttgtgacg catcccatgc ggcaagtgcc tcttcaaccg ctgctgtgtc tgccacacca
      181 atctgtccct ttactcgggg ggaaagatct gctccggaaa gaaccggcag tcctgcctcc
      241 cggaactcct cgattagatg ctgctcctga gcacgggtat agacgtgacg ggggaggata
      301 acagctttta tccgcgcatt ggcgagttcc cgcacaacgc tccgtcccca tccgtctatc
      361 tggaggacat acagtaagtc ctcctcgcca aatcccatct cgtcatcaag actccgtacc
      421 gcatcgcgag atagttgtgg aagaaccttg agagcggaac atccctctcc tgcctgcagt
      481 gcgacgtatc gcttcatacg atccaaccgc acacgcaggt ttttgcacct gcgttcctct
      541 tttctgagct gtttttttac agcggcgatt tcccggtcgc gctcgataat ctcggatcgt
      601 gcaagcagtt ctgtgtgctg catctctcgt tccgcgttga gttttttatg cagggatgag
      661 attgtcttgt cctttcctgt ggattcccgt gaaagtgtct ccaccagtgc tctcagcctc
      721 ctgatggtct cttcctgtcg tgtaatccgt tcatcacgct cgtcgaagat aatctcagta
      781 acagtgtccg tttctttttg tgtctctgtc gttgtcggta tagttccact gctctgagat
      841 agtgcctgtt caagtgagag tccccgtgca atctgcgccc gcagctcatc ggtatcaact
      901 cctgcaggtg ccctcttgag gatactttca aacttgtttt tgtaagtccg gtacgcatac
      961 agtgctgcgg acagcgagtc acgttcgtga tcgtttttga agtcataacc cgctgcaagg
     1021 gcatactttt ctttgatcag gatatctttt ttcgggttcc atgcgattgc ggaaaatgca
     1081 cggcggatct tctccacccc aaccggcatc tctgctttgt cggatgcgat gatgagcggc
     1141 tttccgagtg ttgcaatctc gcgggtcaga tcagccgcgg agaataaccg cgtgcttgca
     1201 aggtagatga gggttccgtc gagatcaagg gctgcaatgc cgatggtagt tccaggatcg
     1261 attccgacga tcaggtacct gggtttcttt gtcagcggtt ggaatgcgat cttgtcgcgt
     1321 ctgcgccctg cgatgcgtat ctgtacgtca ccgcctttgg aaggagaaat tggaatttgt
     1381 tcacgcggtg catagaccgt gaagactgcc cggctttctc ccccgaaagc acggcggacc
     1441 gtcatggagt actcaagatt tgcagagata agtttcatct caatgtcacg ggcatgagta
     1501 cggacggcac cgtgcatctt tcggatgtat cggttctgac tccaccctcc gcgccccggt
     1561 gaccggcagc gggagactgt gatgtcagtt ataccggtgt atgcaaggac ctcgtatccc
     1621 gcgccgaatg atgcgaggag tgctgaagct tttgcctctt ccattggatt ctgcttgtca
     1681 aatgagaggt tgtagcgggc cgccacacgc ggcagtgttt ccatttttat tccgtccccc
     1741 gtaacctgta caagttttgt accagggggc aattcggcaa gaaatgtata cagatctgcg
     1801 gttccctgtg ctatctcctg aatactgtca actgcaagaa tctccggcag ttctgtacgg
     1861 atgtagcgca tgagtcggaa ccgtgtgacg tttttttctt cagagatgac ctctccgtcc
     1921 tctacacgga ctagtgcata gtgcggtcgg atggttccgg acctgaccga tcctcgaatg
     1981 atatcaattc cgaatatgag gctgctcatt tcacctccct gccggtaacg tatcggattg
     2041 catcatcaat gtcatactcc cgctggtatt tttttgcgaa gaatgctgcg acggtttcga
     2101 tgtcatggcg cataaccgca tctgcattca catgatctgg cggaatccac tgcggccagt
     2161 caatgatcca taccactgtg tcgtcataca tgatgttgta ctcggagaga tccccgtgga
     2221 tgaaccctgc atcatatgcg gactttacct gggagaggat ctcctgccag atcttatccg
     2281 gatcttcgaa tctgcaccgg tgcagattta ctcccggaat gaaggacatg acaattacat
     2341 gccggttcat atcgatgggg accgggacgt ttactttccc gttgagtgct ttaagtgcct
     2401 catactcccg ctcggcggat tttgcagagg caaatatcca gggacagtgg gttttttccg
     2461 gcatgtactc acggtttgta cgaacggtct gaaacgatct ctggccgatc ttgtgaagtt
     2521 tcagcacaac cacaccaaat ccaagagcct cataaatttc ggactcctta ccgacaccga
     2581 tcatagtgcc aagcgcagat atggtttttt tctgtgccag ggcagagagt gcgagagcat
     2641 cgtatccttt gaagacaagt gcatatcctt tgtaggggac tgcatcagat ctaaccatgt
     2701 cccggtgaat gaggttacca acacgatatt tggtctctgc tgccgagagg tggacagcgg
     2761 ccttaaggtc gtcttcagga acccaggcat aacggtgcat catcctctcc agtgcacgaa
     2821 ggatgcggac ttcatattta tggaggtttt tgatgtcttc ggctgacaga ggcatgagat
     2881 attggttgac tttgcaggga taaaagtatc atgtccgatg tgccgaagaa cgaatctaca
     2941 tatttgctga acaccaactg agtagtatat gcagtgttcg cggtgcggga aagaggcagt
     3001 gattgtacag ccctactcag gcgtatccct ctgtgccgta catgcagccc gcgatcttga
     3061 agcgaaggca aaacgaagga tccggaagtg cgggagtatc tcgtctgggg aacgactgta
     3121 tgttcaggag tcatttggcc cagaatcatt tgcactctcc gttcttctct caaggattct
     3181 tgcaggccgc cgtgatgttt cgttagtgtc agatccttct gatgcaactg cggtggtttc
     3241 atcggaaaca cttgactcct ccgcctgctg ctttcttcaa cagatcattt cgggaacgac
     3301 gccggagata ctctccctcc cttccaggaa agtacttgat cctctggccg tagttcctag
     3361 ggttgaggtg ttgcagtatg cccgtcacca tggatgggac ggtgtggatg ttcgtccacg
     3421 tttgtccgat ccgtttactg cggatatctg tgcgtttctt gaacacttct cggtggatca
     3481 tccttctgcg gcctatgcac tgatgcaggt ccgggatgcc ctgccccaac tgtatatgga
     3541 gagaaccaat catgctctgt aaggatatcg ggtgtgctat cacccgcata aaagatctga
     3601 tccgtcagac cctctgggct tcgggtgcga aaggtgttgt tgtcggaatt tccgggggta
     3661 ttgactcggc ggtcgcaacg gcgattgcag taaaggccct tgggccggag catgttcttg
     3721 gtgtacatat gccgtctgcg tcaagcagtc ccttggatat tgcagatgcc aaggattttt
     3781 gtgacaaatt tggtattgaa ctgctgactg ttcctctggg agatgttgtt tcagcggcgt
     3841 tttctgatcc atacctgatg gatactccga tacttcgcgg gaattacact tcccgcctgc
     3901 gaatggcaac tctctataat attgcggcag cccggggctt tctggtctgt gggacctcaa
     3961 acaagaccga gtacatgatc ggttac
//
LOCUS       DALUAJ010000086         3061 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_15299,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000086 DALUAJ010000000
VERSION     DALUAJ010000086.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15299"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="O0X37_01860"
     CDS             <1..516
                     /locus_tag="O0X37_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4013 domain-containing protein"
                     /protein_id="HJK61708.1"
                     /translation="RVYRGGEAKLGEWIKMLIDGILLWIIGIIYSIVPFIVLVIFGGA
                     ALLNPFVLMDPNAAETAFAGISIIGLIIALIVSIIFGIFGSIASVRFAKEESLGAAFQ
                     LGEIINIIGKIGWIHYILSVIVLAIIFAVIGFICGLLTIILIGIILLLILMPLLLIWS
                     ARFVANLYESA"
     gene            594..1067
                     /locus_tag="O0X37_01865"
     CDS             594..1067
                     /locus_tag="O0X37_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61709.1"
                     /translation="MNYKLVTVCVLLLAALVFVAGCVDSNDPSETPTPTPTVTPTVNP
                     DFSLTPGPTDEFPAGKDVTVQIGRDSNKPTITATYAGGKGTNQVSRIEVTLYRADDSQ
                     IITQQFGKEPKIGTELVFEGSSTPSTSDRVKVVAYYYSGEVVVVSDDLYTYKNRT"
     gene            complement(1086..1763)
                     /locus_tag="O0X37_01870"
     CDS             complement(1086..1763)
                     /locus_tag="O0X37_01870"
                     /EC_number="1.1.1.302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone
                     5'-phosphate reductase"
                     /protein_id="HJK61710.1"
                     /translation="MRPYVIINAAMSADGKISTKEHRQTKISGDQDFARVDRLRADCD
                     AVMVGIGTILADDSSLRLKNPDLIAERAAAGKPEQPMRVVIDSQARMPPDGDMFKKGT
                     GQRVIFVAKSAPADRSAALAKKETVITAGTNTVDLNIVLDKLGEMGVRKLMVEGGATL
                     LWSFTSQRLFDEIRIYVGALIIGGADAPTFVDGFGFTRSEGFTRLTLKDVEKIDDGLL
                     ITWLKKE"
     gene            1946..2911
                     /locus_tag="O0X37_01875"
     CDS             1946..2911
                     /locus_tag="O0X37_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HJK61711.1"
                     /translation="MPNLTVAVLAPNGYARDIGKKGTSTDITFYNLKKGHDTVTLLEP
                     TRYPDRLAPLFYVVSMAHEAIVVVEEINPMFAEWVLILDCAGVSKGTIILKNYIQPSD
                     IAPLIKGTVLEGYEICEEDFITLSANLLARAAAQPEIEPKEGSVGTVPVDHHFNVRGI
                     GTVILGCVADGWIKKHDKVRVEPIGKTAQIRSVQKHDDDFAWAYAGDRVGCALKDIEA
                     EDLDRGFVLTTDPQVKSSTTITGPAELVKYWPAAVKEQMVISVGHWMQFLAARVTAVD
                     NGSDWHKPVLTIEMETPLVYKPGDKAVLHYLDGGKLRIMGTIILP"
BASE COUNT          770 a          800 c          748 g          743 t
ORIGIN      
        1 cgtgtatacc gcggcggaga agcaaagctc ggcgaatgga tcaagatgct gattgatggt
       61 atcctgctgt ggattattgg tattatttac agtattgtac cgttcattgt gctggtaatc
      121 ttcggtggtg cagcactgct gaatccgttt gtcctgatgg accccaatgc agcagaaacc
      181 gcttttgcag gcatcagtat cattggtctg atcattgcac tgatcgtctc catcatcttt
      241 ggaatcttcg gatccattgc atctgtccgg tttgcaaagg aggaaagtct gggtgcagca
      301 ttccagctcg gcgagatcat taacatcatc ggcaaaatcg gatggattca ctacatcctg
      361 tccgtgattg ttcttgcaat catctttgcg gtgattggtt tcatctgcgg actgctgacg
      421 atcatcctga ttggaatcat cctgctcctg atccttatgc cgctcctgct catctggtcc
      481 gcacggtttg ttgcaaacct ctacgagtcc gcataagcag gattgcaaat aacccttttt
      541 ttctttccgg ttcccagcgt aaattataaa cgtggacagg acgattttag agtatgaact
      601 acaaacttgt gaccgtgtgt gtcctgcttc ttgcagctct ggtttttgta gcgggatgtg
      661 ttgattccaa cgacccctca gaaacaccta caccaacacc aacggtaacg ccgacggtca
      721 acccagactt cagtctgacc cccggaccca ccgatgagtt cccggcagga aaggacgtaa
      781 cagtacagat tggccgtgac tcgaacaaac caaccatcac cgcaacctat gccggtggta
      841 aaggtacaaa ccaggtcagc agaatcgagg ttaccctcta ccgtgcggat gatagccaga
      901 tcattaccca gcagtttggc aaagagccaa agatcggaac tgaactggtc tttgagggat
      961 cctccacacc ctctacaagc gaccgtgtaa aggttgttgc ctattactac tccggcgagg
     1021 ttgtggttgt aagcgatgat ctctatacct acaagaacag aacgtaaaac agaaaaaaat
     1081 tatttttatt cttttttgag ccaggttatc agaaggccgt catcgatttt ctcaacatct
     1141 ttgagagtaa gacgggtgaa tccttcgctg cgggtaaatc caaagccgtc cacaaaggtg
     1201 ggggcatctg caccgccgat gatcagtgcc cctacataga tgcggatctc atcaaagagt
     1261 cgctggctgg taaatgacca cagaagcgtg gccccgcctt ccaccatcag ttttcgaaca
     1321 cccatctccc cgagtttgtc cagaacaatg ttgagatcca ctgtgttggt tccggcagta
     1381 atcacggttt ccttctttgc aagagctgcg ctacgatctg caggtgcgga ctttgcaaca
     1441 aaaatcaccc gctgaccggt ccctttcttg aacatgtccc cgtccggcgg catccgtgcc
     1501 tgactgtcga tgacaacacg catcggctgt tccggttttc cggcggcagc acgctccgca
     1561 atcagatcag gatttttaag acgaagcgag gagtcatcgg cgagaatggt tccgatacca
     1621 accataactg catcgcagtc cgccctcaga cggtccacac gtgcaaagtc ctgatcgcct
     1681 gaaattttgg tctgccggtg ctctttggtg gatattttcc cgtcagcact cattgcagca
     1741 ttgatgatga cgtagggacg catgtatgtc cctttgtgtt tgagggatat atacgttgca
     1801 ggtttgggaa actacgggat caagattgaa gaagagaaaa ggcagacact ctgtactccg
     1861 ctctctgttt tagagaagag gattctgttc caactggacg atcattgaaa gagttaatcc
     1921 cccgcaacgt ccaatacata cggatatgcc aaacctgaca gttgcagtcc ttgctcccaa
     1981 cggatatgcc cgggatatcg ggaaaaaggg aacaagcacc gatattacct tctataacct
     2041 gaaaaaagga cacgataccg ttacactgct ggaaccaacc cgctatccgg acagacttgc
     2101 cccgctgttt tacgtggtct ccatggcaca cgaggcaatt gtcgtcgtgg aagagatcaa
     2161 ccccatgttt gcggagtggg tgctgattct tgactgtgca ggtgtctcca aagggaccat
     2221 catcctgaaa aactacattc agccctcaga cattgcgccg ctgatcaaag gtacggttct
     2281 tgaaggatac gagatctgtg aggaggattt cattaccctg tccgcaaacc ttcttgcccg
     2341 tgcagcagca cagccggaga tcgagcccaa agagggatcg gtcggaaccg taccggttga
     2401 tcatcacttc aatgtgcggg gtatcggaac cgtcattctc ggctgcgtgg cagacggctg
     2461 gatcaaaaag catgacaaag tacgggttga gccgatcggc aaaaccgcac agatccgatc
     2521 agtacagaaa cacgacgatg acttcgcctg ggcatacgct ggagaccgtg tcggatgtgc
     2581 actgaaggat atcgaagccg aggatctgga ccgcggattt gttcttacaa cggatccgca
     2641 ggtgaaaagc tccacaacga tcacaggacc cgcagagctg gtgaagtact ggcctgcagc
     2701 agtaaaagag cagatggtca tctcggttgg tcactggatg cagttccttg ccgcacgggt
     2761 gaccgcagtg gacaatggtt ctgactggca caaaccagtg cttaccattg agatggaaac
     2821 cccgcttgtc tacaaaccgg gagacaaggc ggttctgcac tatctggacg gaggaaaact
     2881 ccggattatg ggaacaatta ttcttcccta accttttttt ctactctaaa aatagtcggc
     2941 gtcctccgca cccacacact catattctcc cggcaacaaa ctaacagaca caacacatgt
     3001 ccaccgccga ataccgctat catctcgcag aatttcccgc gccgaatgtt tctgtccgcc
     3061 a
//
LOCUS       DALUAJ010000087         2550 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_15344,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000087 DALUAJ010000000
VERSION     DALUAJ010000087.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15344"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="O0X37_01880"
     CDS             <1..443
                     /locus_tag="O0X37_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HJK61712.1"
                     /translation="IDKFAGMPDGKAKLEAIEHRPPAEVIDDEYPIWLTTGATIWHWP
                     SGSMTRRCEQLDRDAPTAWLEMNPADAAKKGIADGEKVILYSRRGEVAVNARITPLIK
                     EGVFFMPFHFTEARVNLVTNTAYDPVSRTPEFKVCAVNVKKMEA"
     gene            446..1681
                     /locus_tag="O0X37_01885"
     CDS             446..1681
                     /locus_tag="O0X37_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HJK61713.1"
                     /translation="MSAKGDMYYAWTKSDIKGECGGAVISLLKYALENKIVDVVLTVQ
                     KGIDIYDPKPVFITNPADLDACAGSLHCGTLLLPKLIKKYLNGAKDIKVAVTCKGCDA
                     KALLELGKRQQINLDNVISIGLNCGGSVSPMPARRMIREKYGVNPDDVVKEEIDKGKF
                     IIMTKDGEHKGISIEELEEEGYGRRKNCQRCETKIPRQCDLACGNWGVIGEKAGKATF
                     VEICSEKGAMLFDAAQKAGAIDSCAPEAKGLEIRGKIENVMIKMGQKNQEKQFAELGT
                     GTERLNKIMQQSSKCIKCYGCIENCPICYCVECSTKKPHLVRPGIIPPDFMFQMIRFA
                     HIADSCINCGQCTELCPMDIPNSLYMHAQQVELEKMFGHKPGYDMTMPVLSYAEEMEE
                     RARLNATGSDMIFENVFKE"
     gene            1781..2368
                     /locus_tag="O0X37_01890"
     CDS             1781..2368
                     /locus_tag="O0X37_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HJK61714.1"
                     /translation="MIDSFIEGNKIFLEQDFERKKDRYMTLVESQHPTVLWIGCSDSR
                     VNAERITHCRAGELFTHRNIGNIVPTHDWNFATVLEYAVRHLKVKDIVICGHSDCGAL
                     KALDADMTGDAYIPMWINNAREAQVRVDARRGKPATTPEEKAARKKEIEIENIRLQME
                     HLRTYPLVTEAEKNGKLEVHGLYYDLNTGVLSKIA"
BASE COUNT          656 a          638 c          662 g          594 t
ORIGIN      
        1 acattgacaa gttcgcaggc atgcctgacg gcaaagccaa actcgaagca atcgagcacc
       61 gcccgccggc agaagttatc gacgacgagt atccgatctg gctgaccacc ggtgcaacca
      121 tctggcactg gccgagtgga tccatgaccc gcagatgtga gcagcttgac cgcgatgcac
      181 caaccgcctg gcttgaaatg aacccggcag acgccgcaaa gaagggtatc gcagacggcg
      241 agaaagttat tctttacagc cgccgcggcg aagtagcggt caacgcccgt attacacctc
      301 tcatcaaaga aggtgtgttc ttcatgccgt tccacttcac cgaagcccgt gtaaacctcg
      361 ttaccaacac tgcatacgat ccagtatccc gtactccgga gttcaaggtc tgtgctgtga
      421 atgttaagaa gatggaggcc taaaaatgtc agcaaaaggc gatatgtact acgcatggac
      481 caagtccgat atcaaaggag agtgcggagg agctgtcatc tcccttctga aatatgcact
      541 tgagaacaag atcgttgatg ttgttctgac tgtgcagaag ggcattgata tctacgaccc
      601 gaaaccggtc ttcatcacta accctgcaga ccttgatgcc tgtgcaggtt ccctccactg
      661 cggaaccctg cttctgccca aactcatcaa gaagtacctg aacggtgcaa aggacatcaa
      721 agtcgctgtc acctgcaaag gctgtgatgc aaaagcactt cttgagcttg gcaaacgcca
      781 gcagatcaat ctggacaacg ttatctccat tggtctgaac tgtggtggtt ccgtttcccc
      841 gatgccggcc cgcagaatga tccgcgagaa gtatggtgtc aaccccgacg atgtcgttaa
      901 agaggagatc gacaagggta agttcatcat tatgaccaag gacggcgagc acaagggtat
      961 ctccattgag gagctcgaag aggaaggcta cggccgccgt aagaactgcc agcgctgtga
     1021 gaccaagatt ccgcgccagt gcgatctcgc ctgcggaaac tggggtgtca tcggtgagaa
     1081 ggctggaaag gcaacctttg ttgagatctg ctcggagaag ggtgcaatgc tctttgatgc
     1141 agcacagaag gcaggcgcaa ttgactcctg tgcacctgag gcaaagggtc ttgagatccg
     1201 cggtaagatc gagaacgtta tgatcaagat gggacagaag aaccaggaga agcagttcgc
     1261 agaacttgga actggtactg agagactcaa caagatcatg cagcagtcct ccaagtgtat
     1321 caaatgctac ggctgtattg agaactgtcc gatctgctac tgtgtcgagt gttctacgaa
     1381 gaagccgcac ctggttcgcc cgggaattat tccgccggac ttcatgttcc agatgatccg
     1441 ctttgcccac attgctgact cctgtatcaa ctgtggtcag tgtactgagc tttgtccgat
     1501 ggacattccg aactcgctct acatgcacgc ccagcaggtt gagctggaga agatgttcgg
     1561 tcacaagccg ggttacgaca tgacgatgcc ggtcctctcc tatgctgagg agatggaaga
     1621 gcgtgcacgt ctcaatgcaa ccggctcgga catgatcttt gaaaacgttt tcaaagagta
     1681 atctctctca caactttttc cggatgcgtt gccggtctgt cgtgagacag accgtgtgcg
     1741 tctcctcttt ttcacaatag acaacaaggt ggtactgaaa atgattgata gtttcatcga
     1801 aggcaacaaa atatttctcg aacaggattt tgagcggaag aaggatcggt atatgactct
     1861 ggtcgagtcc cagcacccga cggttctgtg gatcggttgt tctgattctc gtgtcaatgc
     1921 cgaacgcatt acacactgtc gtgcaggaga gctgtttaca cacagaaaca tcggcaacat
     1981 tgttccgacg catgactgga actttgcaac ggttctggag tatgcggtcc gtcacctcaa
     2041 agtcaaagat attgttatct gtggtcattc cgactgtggt gcactcaaag cacttgatgc
     2101 agatatgacg ggtgatgcct atattccgat gtggatcaac aatgcccgag aagcccaggt
     2161 gcgtgttgat gcacggcgtg gtaaaccggc caccactccc gaggaaaaag ccgcacggaa
     2221 aaaggagatc gagatcgaaa atatccgtct ccagatggaa catctcagaa cgtaccctct
     2281 ggtcactgaa gcagagaaga acggaaagtt agaagtccat ggattatact atgatctcaa
     2341 taccggcgtt ctttccaaga tagcctgagt gtttttgatc atcttctttt ttatcttgca
     2401 tcgccggtga aaatattact cagcgttttc ctgcgcacgg cggtctcccc tgatattgcg
     2461 gtagcttttt tgcgagaagt gtaaccgctt cctctggtgt atccacgccg aatatccgat
     2521 catcagggat atctgggcac cgttccttat
//
LOCUS       DALUAJ010000088         2562 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_15539,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000088 DALUAJ010000000
VERSION     DALUAJ010000088.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15539"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(79..1260)
                     /locus_tag="O0X37_01895"
     CDS             complement(79..1260)
                     /locus_tag="O0X37_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF373 family protein"
                     /protein_id="HJK61715.1"
                     /translation="MTAGRTLILSVDRDDDIGYKAGVMSPAVGREACLDAATRLGIAD
                     PEDSDTNAIFQAIKTYDELAAKGADVEVAVIGGNHMNMLEGDRRIAELLEKVVAETGV
                     ENCILISDGAEDEFILPIIQSQLRITSVVRVVIKQLPNIEGTYYIIKKLFNDPKVARG
                     VLVPIALAMILYVVASLVSDEVPAMLVVIGIIGVYLLIKGFGLDEYIGYIYYGLVDSF
                     HKGRISFVGYIIAGIMAICGAIAGLMSIIVYYPISGDAGLLYNIMTFLYGAIIWMMGA
                     GLLAAASKIADCVQNEQKALARMFVVPFFVVAIGLIMYGAVIYFLSISPVEPFPFDVT
                     EGIAAIILLTIAGLIVAFTGIYYRPAVQRYVLDWIERKMQQDAEAETKGRAPVYKKIK
                     Y"
     gene            complement(1393..2274)
                     /locus_tag="O0X37_01900"
     CDS             complement(1393..2274)
                     /locus_tag="O0X37_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HJK61716.1"
                     /translation="MTEDLVEKRKTLLEESEEHKAKRNELNAQASQFARERNELNNAT
                     RQYVEDAQKNKELRDQYNNDVQALKEQRNELNDKANALFEEIDALRGDAGSGAAAASH
                     EKKASVKDILRQIESLEERQQTEVMTTEKERELVDKIKQLRAEVKEQEVEHEQNKEVR
                     SRLIEAREYRKQASALHADVTEKAELAQKHHDLMVECYRKADKSREGADAKHKQFVEA
                     QEAADAEHKAFLECQKNLRDYDKVLTGMRSKQKKVKTVKENKSARKEAETIFNAFKSG
                     EKLTTEDLLKLQRSKLI"
BASE COUNT          571 a          714 c          601 g          676 t
ORIGIN      
        1 agttagatta ctgcctcttt ggaaaaaagt attcctcaca gcagcgggat ttcctgtaga
       61 ttttttcaaa aaagaagctt aatatttaat cttcttatac accggcgcac ggccttttgt
      121 ctcggcctcc gcatcctgct gcatcttgcg ctcgatccag tcaagcacat accgctggac
      181 cgccggccgg tagtagatgc ccgtaaacgc cacaatcagt ccggcaatgg tcagcagaat
      241 aattgccgca ataccctcgg tgacatcgaa cgggaacggc tccaccggcg agatcgacag
      301 gaagtagatc accgcaccgt acatgatcag cccgatcgcc accacaaaga acggcaccac
      361 aaacatccgg gcaagtgcct tctgctcgtt ctgcacacag tccgcaattt tacttgcagc
      421 cgcaagaagg cctgcaccca tcatccagat gatcgcaccg tacaggaacg tcataatatt
      481 gtagagcagt cctgcatcgc ctgagatcgg gtaatagacg atgatactca taaggcctgc
      541 gatggctccg cagatcgcca taattcctgc gatgatgtag cctacaaacg atatacgccc
      601 tttatggaag gagtcaacaa ggccgtagta aatgtagccg atgtactcgt caagaccgaa
      661 tcccttgatc agcagataca caccgatgat gccgatgaca accagcattg ccggaacctc
      721 atccgagaca agcgatgcga ccacatacag aatcatcgcc agtgcaatcg gcaccagaac
      781 accgcgggcg accttcgggt cgttgaacag cttcttgatg atgtagtagg taccctcaat
      841 gtttggcagc tgcttgatca ccacccgcac cacactggtt attctcagtt gtgactgaat
      901 gatcggcagg ataaactcat cctccgcacc gtccgagatc agaatacagt tctccactcc
      961 ggtctcggca accaccttct ccaaaagctc cgcaatcctg cggtcacctt ccagcatatt
     1021 catgtggttt ccgccgatca ccgcaacctc gacgtccgca cctttggcgg caagttcgtc
     1081 gtaggtcttg atggcctgga agattgcgtt tgtgtccgaa tcttcggggt ccgcaattcc
     1141 gagccgcgtt gctgcatcca gacaggcctc tctcccgacg gcagggctca tcactcctgc
     1201 tttatatccg atgtcatcgt cacgatcaac gctgagaatc agagtccgtc cggcagtcat
     1261 tgactatacg tttgcgagtt ctttgataat atacctgtgg gagacaaatg tcagacatac
     1321 gcccaacttt tgcacaaaac agcgttgtaa caccgggacc ggtcgtcccg caaaacagaa
     1381 aaaaatatgg atttagataa gcttcgaccg ctggagcttt aacaggtcct cggtcgtcag
     1441 cttctcgccg ctcttgaaag cgttgaagat ggtctctgct tccttgcgtg cagacttgtt
     1501 ctccttgacg gtcttgacct tcttctgctt ggaacgcatg ccggtcagga ctttgtcgta
     1561 gtcgcggagg ttcttctggc actcaaggaa tgctttgtgc tctgcatctg ctgcttcctg
     1621 agcctcgaca aactgtttgt gctttgcgtc tgcaccctcg cgtgacttgt cggctttgcg
     1681 gtagcactca accatcaggt cgtggtgctt ctgtgcaagc tctgccttct cggtcacatc
     1741 tgcgtggagt gcggatgcct gcttgcggta ttcgcgggcc tcgataaggc ggctgcgaac
     1801 ttccttgttc tgctcgtgct caacttcctg ctctttgact tcggcacgga gctgcttgat
     1861 cttgtcaaca agctcgcgct ccttctccgt cgtcatgacc tcagtctgct gtctctcttc
     1921 aagagactca atctgacgga ggatgtcctt tacggatgcc ttcttctcat ggcttgcagc
     1981 ggctgcgccg gaacctgcat cgcctctgag tgcgtcaatc tcctcaaaga gtgcatttgc
     2041 cttgtcgttt aactcattgc gctgttcctt cagagcctgg acgtcgttgt tgtactggtc
     2101 acgaagttct ttgttcttct gtgcatcctc aacatactga cgggttgcat tgttcaactc
     2161 gtttctctcg cgtgcaaact ggcttgcctg cgcgtttaac tcgttgcgtt ttgctttgtg
     2221 ttcttcggac tcttcaagga gtgtttttct cttttcgaca agatcttcgg tcaacgttct
     2281 tacctttcct gagtagtcgt ctgtcagatc agagcgggga tgtgcatgcg gaaccggaca
     2341 cgaaatcaaa tacaaagctg catgaagaat gacacaaaaa cgaatctaaa atttcaagcg
     2401 aacgaatggt attcaacctc ctgtactttt tgccgtatgc cttttgcttc tgctctgcag
     2461 atatacgact ttcctataat aaatagcgct atgctgattt aaacatatcg aaaatccgaa
     2521 agacacaagt tccaacccgg cggcgggttg gggttttgag gg
//
LOCUS       DALUAJ010000089         1940 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_15564,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000089 DALUAJ010000000
VERSION     DALUAJ010000089.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15564"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..84)
                     /locus_tag="O0X37_01905"
     CDS             complement(<1..84)
                     /locus_tag="O0X37_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866167.1"
                     /note="in yeast this protein is involved in start site
                     selection during the initiation of translation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HJK61717.1"
                     /translation="MNSGICPKCGLPKELCICEEVAKEQQRI"
     gene            complement(187..822)
                     /locus_tag="O0X37_01910"
     CDS             complement(187..822)
                     /locus_tag="O0X37_01910"
                     /inference="COORDINATES: protein motif:HMM:NF021414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2115 family protein"
                     /protein_id="HJK61718.1"
                     /translation="MGFWNRFGAEDRTVCGVDWNALARISRKGDLLAQLKSYGMLFTP
                     DDLEEMLVRYGKKLVHVKKEYATALLASARVQIVDGYHRMMIAELASVHAEVRLPKSW
                     HGFVQSAAVQTSGKRLAELKYLIAAYTMYIAEEPAHPVGTPFPGGYTVELHEGVYYCP
                     VRAAQSEVDETLCRFCPAVQSRERDLVLTREQREFVERGEKLENYFYNYHG"
     gene            complement(823..>1940)
                     /locus_tag="O0X37_01915"
     CDS             complement(823..>1940)
                     /locus_tag="O0X37_01915"
                     /inference="COORDINATES: protein motif:HMM:NF028231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HJK61719.1"
                     /translation="GVVESGVFYPETKRAQARETLIRLCKMHAIDIIAIGNGTAGRET
                     ERFVKSISFGRPLPVLLTSECGASVYSASAEARREFPDLDITLRGAVSIGRRVQDPLA
                     ELVKIDPKSLGVGQYQHDVDQNRLAAKLADVVEQTVNAVGVNLNTASPALLSYVSGIG
                     PVLAEKIVAYRTEHGPFKNRRELLDVPGVGPKIFEQAAGFLRIRNGDNPLDLTGVHPE
                     RYPVVEQMAADLGVLPGRLVGGTELLKRIEPERYVSPDCGLVTITDILEELANPGRDI
                     RVTGEEEYSFDPAVHAITDLAEGMVLPGIVTNVTAFGAFVDVGVHQDGLVHISELADR
                     FVAHPSEIVTVGDRTRVVVLAVDISRSRISLSIKRAV"
BASE COUNT          506 a          538 c          438 g          458 t
ORIGIN      
        1 gattctttgc tgttcctttg cgacttcttc acaaatacag agttcttttg gtaaaccaca
       61 ttttgggcag atgccactgt tcattgcact actaaatggt gtccgtgagt taaaatagat
      121 attggcagga tgggctgaga tgaaaaaaca aaaaccgtag aagaaaatcc gtctttattt
      181 tatgactcac ccatgataat tgtaaaaata gttctccagt ttctccccgc gctccacaaa
      241 ttcacgctgt tcgcgggtaa gcacaagatc tcgttcacgg ctctgtactg ccggacagaa
      301 ccggcagaga gtttcatcaa cctcactctg ggctgcacgg acagggcagt agtatacacc
      361 ctcatgcaac tcaacagtat aacctcccgg aaacggtgtt ccaaccggat gggccggttc
      421 ctctgcgatg tacatggtgt atgctgcaat cagatatttg agttccgcaa gccgtttccc
      481 gctggtctgt acagctgctg attgcacaaa cccgtgccat gatttcggta gacgaacttc
      541 cgcatgaacg gacgcaagct cagcgatcat catacgatgg taaccgtcca caatctgaac
      601 acgggctgaa gcaaggagag cagttgcata ctcttttttt acgtgtacga gttttttacc
      661 gtaccggaca agcatctctt caagatcatc gggagtaaac aacattccgt acgactttaa
      721 ttgcgcaaga agatctcctt tgcgcgagat acgtgcaagg gcattccagt caacaccaca
      781 gacagtacga tcttctgctc caaaccggtt ccagaacccc atttacaccg cccgctttat
      841 actcagacta atgcggctgc gggagatatc aaccgccaga accactacac gtgtccgatc
      901 tcccactgtc acaatctccg aaggatgggc aacaaagcga tcagcaagtt cgctaatgtg
      961 tacaagcccg tcctgatgta cccccacatc cacaaatgcg ccaaatgcag taacatttgt
     1021 aacgattccc gggagtacca ttccctcagc aagatcagtt atcgcatgaa cggccggatc
     1081 aaaggagtat tcttcctctc cagttacacg aatatctcgg ccagggtttg ccaactcctc
     1141 aagaatatca gtgatggtca caaggccgca gtccggactg acatatctct ccggttcgat
     1201 ccgtttcagg agttctgtac ctccaacaag ccggcctggc aaaacaccaa gatcggctgc
     1261 catctgttcc acaactgggt accgttcggg atggacaccg gtcagatcaa gcgggttgtc
     1321 accattcctg atacggagaa atcccgcggc ctgttcaaaa atcttgggac cgacacccgg
     1381 cacatccaac agttccctgc ggtttttgaa cggaccatgt tcagtacgat aggcaacaat
     1441 tttttcggca agcacaggac caatgcccga gacgtaagaa agcagtgcag gacttgctgt
     1501 attgagattg actcctacag cgttgaccgt ctgctcaaca acatcggcga gttttgcggc
     1561 gagccgattc tgatccacat cgtgctggta ctgaccgaca ccaagtgact tcggatcaat
     1621 cttaacgagc tctgccagtg gatcctgtac acggcggcca atggacacag caccacggag
     1681 agttatgtca agatctggaa actcgcggcg ggcttctgcc gacgccgagt acacagatgc
     1741 accgcactca cttgtcagca gcactggaag tggtcttcca aaggagatac tcttaacaaa
     1801 tctttccgtt tcacggccag cagttccgtt tccgatcgcg ataatatcga tcgcatgcat
     1861 tttacagagc cggatcaggg tctcacgtgc ctgagcacgt ttcgtctccg gatagaaaac
     1921 accgctctct accacaccac
//
LOCUS       DALUAJ010000090         3305 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_15575,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000090 DALUAJ010000000
VERSION     DALUAJ010000090.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15575"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="O0X37_01920"
     CDS             <1..207
                     /locus_tag="O0X37_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HJK61720.1"
                     /translation="MYISGTITSAHVHPEHVVSALSPDSDGFMTVEVLDGCIVARVEG
                     ESLRSVIATVDDYLMNLSVSEQLC"
     gene            259..879
                     /locus_tag="O0X37_01925"
     CDS             259..879
                     /locus_tag="O0X37_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3ae"
                     /protein_id="HJK61721.1"
                     /translation="MARKKQSGGRKVEGWKAKSWFKVYAPEFLGKQFIGEIVSSDTAN
                     LPGRVLSVSLGELIQDYSKQNVKISFKINDVAGDAAYTQFHGHEMTKDFIRAMVKRRA
                     SRIDSTITIQPLGSVRELHVTITAFTINHARLSQIHEIRAMMVKVVQDYAKEADFETF
                     VSAMVKGELSKKMLEACKPIFPIRKIEVIKSESVSSAAERAAALVR"
     gene            complement(1052..1882)
                     /locus_tag="O0X37_01930"
     CDS             complement(1052..1882)
                     /locus_tag="O0X37_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HJK61722.1"
                     /translation="MKSIFSEPLHILFVDTACIRCGLCATMCTTGKIVASETKSPKIC
                     DDAPCLSCGQCIAVCPQAALSTDTPVFVTPADNSAYKTGINPDLLSTYLKSRRSVRIW
                     KEKEVAQKDLEQLIGIAAYAPSACNIHPVKWVVISDPATIRKFTDASIAALKALPPDH
                     PLAQLSQMLIANADNGSDPICRSAPALLIAASDADQEYGLIDSIIALSYIDAFAPSIG
                     LGTCWVGYVMIMLHIKPELGEILGIPKNWVPQYAMLAGYPGVSFSQIPPREIPEVIWK
                     "
     gene            complement(2047..2131)
                     /locus_tag="O0X37_01935"
     tRNA            complement(2047..2131)
                     /locus_tag="O0X37_01935"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2093..2095),aa:Leu,seq:cag)
     gene            complement(2214..2747)
                     /locus_tag="O0X37_01940"
     CDS             complement(2214..2747)
                     /locus_tag="O0X37_01940"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HJK61723.1"
                     /translation="MVFDCGANLGVFTVLAAYRGAEVFAFEPIPEARTILRETLKLNP
                     AFADRVHVIPCALGDEEKTAEFTILEDTLVGSSMVLPQTGRKMRVPVTTVDAFVEAEG
                     LDAVNFIKADIEGAERQMLAGAGDTLRNYTPKIAICTYHLADDPLVIANLLRNADPRY
                     QLISRWKKMYGVVPSKR"
     gene            complement(3053..>3305)
                     /locus_tag="O0X37_01945"
     CDS             complement(3053..>3305)
                     /locus_tag="O0X37_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HJK61724.1"
                     /translation="LVFQLIIAVILMVPSTPGGAGVAEISYAAFYSLVLPSAVVGIFV
                     VLLRLILYYSNLLIGFVASFRIVKREATDEMVKLEEEGV"
BASE COUNT          851 a          779 c          805 g          870 t
ORIGIN      
        1 atgtacataa gcgggacaat cacgtcagcc catgttcatc cagagcatgt agtctcggca
       61 ttgtctcctg actcggacgg gtttatgact gttgaggttc tggatggttg tattgtggca
      121 agagtagagg gagagtcgct tcgatcggtg attgcgacgg tggatgatta tctgatgaat
      181 ttatccgtct cggaacagtt gtgttagatg cacggtttcc gggtacaaaa atacaatttc
      241 aaaaattaag gtgataccat ggcaagaaag aagcagagtg gtggacgcaa agttgaggga
      301 tggaaggcaa agagctggtt caaggtttac gccccggagt tcctcggcaa gcagtttatt
      361 ggggagatcg tctcctctga cacggcaaac ctgccgggtc gtgtactgtc ggttagtctt
      421 ggtgagttaa ttcaggacta ctccaagcag aatgtgaaga tttcgttcaa gatcaatgat
      481 gttgcaggag atgcagcata cacgcagttc cacggccacg agatgaccaa agatttcatc
      541 cgtgcaatgg tgaagaggcg ggcatcccgt atcgacagca ccattacgat ccagccgctt
      601 ggcagcgttc gtgaacttca tgtgacgatt accgccttca ctatcaacca cgccagactt
      661 tcccagattc acgagattcg tgcaatgatg gtgaaggttg tgcaggacta tgcaaaggaa
      721 gcagactttg agacctttgt ctccgcaatg gttaagggtg agctttccaa gaagatgctc
      781 gaggcatgca agccgatctt cccgatccgt aagatcgagg tcatcaagtc cgagtctgta
      841 agttctgcag cagaacgtgc agcagcactt gtccgttaaa tcaacggata cttttctttt
      901 ttaattctct tgtgacaacc gagacccatt ttttcacgat agatgggcga tctcagtctg
      961 aaatataatt tctaggataa atgatgagtg aaccgatgtg tcctgttttt tttgcagtag
     1021 tatttgttgc atgttggaaa atgtaaatgc attatttcca gataacctcc gggatttctc
     1081 gcggaggaat ctgtgaaaag gagacaccgg ggtatcctgc aagcatcgca tactgcggta
     1141 cccagttttt gggaattcca agtatctctc ctaactcagg ctttatgtgc agcatgatca
     1201 tgacataacc gacccagcag gtaccaaggc cgattgacgg tgcaaatgca tcaatgtagg
     1261 acagcgcaat gatactgtcg attaatccat actcttgatc cgcatcggac gcggcgatca
     1321 ggagagccgg ggcgctgcgg cagatgggat cggatccgtt gtctgcattg gcaatcagca
     1381 tctgtgagag ttgtgccaga ggatgatcag gggggagtgc tttcagcgcg gcgattgagg
     1441 catctgtaaa cttccgtatc gttgcaggat cagagatcac cacccacttt accggatgga
     1501 tgttgcaggc tgacggtgca tacgctgcaa ttccgatgag ttgttcaaga tccttctgtg
     1561 caacctcttt ttctttccag atacgtactg atctgcggct tttcaggtac gttgacagaa
     1621 gatcagggtt tattcctgtt ttatatgcag aattatctgc tggtgtgaca aataccggag
     1681 tatccgtgga taacgctgct tgaggacaaa ctgcaataca ttgtccgcag gagagacagg
     1741 gtgcatcatc acagattttt ggggactttg tttctgacgc gacgatcttg ccggtcgtac
     1801 acattgttgc gcacaacccg catctgatac aggcagtatc aacaaagagg atgtgcaatg
     1861 gttcggaaaa tattgacttc atcagaaata ctctggaacg tgcgggtgat agagttttgg
     1921 gttctgacac cctctggact atcggtctat ctgttagtta tgatatactc acataaggtt
     1981 catggtttgg caaactggaa aaaaatcctc gcgtattgta cttttccaaa atggtacgac
     2041 aaacagtgcg aaggaaggga ttcgaacccc agaactccta tgagaccaga ccctgaatct
     2101 ggcgccgttg acctggcttg gctaccttcg catgaaaata gaaaacatcc tgcaatcagg
     2161 attgtcttcg tgccctatta tatttgtgat tctcatactt aaatttaggc atattatctt
     2221 ttagatggaa ccaccccgta catctttttc catcgtgaaa tcagttggta ccggggatcc
     2281 gcattccgga gcagattagc aatcacaaga ggatcatccg caagatgata ggtacagatg
     2341 gctatctttg gggtgtagtt tcgcagcgta tcccccgctc ctgcaagcat ctgccgctct
     2401 gccccttcta tatctgcttt gataaaattc accgcatcca gcccttctgc ctctacaaat
     2461 gcatctactg ttgttaccgg aacacgcatc tttctgcctg tttgtggtag taccattgac
     2521 gatccgacca gtgtatcttc aagaatagta aactccgcag ttttttcctc atcacccagt
     2581 gcgcagggga tcacatgtac ccggtcagca aaggccgggt tcagctttag cgtctctctt
     2641 aggatcgttc tggcctcagg tatgggttca aatgcaaaca cttcggctcc acggtatgcg
     2701 gcaagtacag taaacacacc gagatttgca ccgcaatcaa acaccacatc atcacgcgca
     2761 atccgtacat ctccccactc atacggcccc tagtccacat acccatacct ctgaaacaaa
     2821 gctgggatga gaatatctcc tatctccatt gcgtaatatg catcctcgcg tgttgggtgt
     2881 tccgatacgg acagacgcgg tagatggagc ggtccgatac gcagacatct ctcctctgag
     2941 gagtagaatc gtgcaaaaaa ccgttcatac tggtagcggt aaaaatcctc ggtgcggtct
     3001 ccctttgtca gaatgtatcg gagcctatcg cgcaggggaa tataatcccg gttcagactc
     3061 cttcctcctc aagttttacc atttcatctg tggcttcacg tttcacgatc cggaaacttg
     3121 caacaaaccc gatgagaagg tttgagtagt acagaatcag ccgcagcagt actacaaata
     3181 ttccgactac tgctgacgga agaaccagcg aataaaatgc ggcatagctg atctccgcca
     3241 ctcctgcacc gcctggtgtt gacggaacca tcagaatcac tgcaataatc agttgaaaca
     3301 caaga
//
LOCUS       DALUAJ010000091         2610 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_15605,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000091 DALUAJ010000000
VERSION     DALUAJ010000091.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15605"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="O0X37_01950"
     CDS             <1..367
                     /locus_tag="O0X37_01950"
                     /inference="COORDINATES: protein motif:HMM:NF017913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HJK61725.1"
                     /translation="LVVAALMGVAETVTLVMTALQDPTQHALSNVLQAILLIIVIATL
                     VDMVASYVRAGRVLVRPILIAGITTMVRRLLVSDLTFIDIIGTTIVILGLTVAMVYVG
                     RDEMEMMEHLKDMKRSEDQ"
     gene            454..526
                     /locus_tag="O0X37_01955"
     tRNA            454..526
                     /locus_tag="O0X37_01955"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:488..490,aa:Pro,seq:ggg)
     gene            555..1412
                     /locus_tag="O0X37_01960"
     CDS             555..1412
                     /locus_tag="O0X37_01960"
                     /inference="COORDINATES: protein motif:HMM:NF014046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ARMT1-like domain-containing protein"
                     /protein_id="HJK61726.1"
                     /translation="MRLAPECRACLLSRVVAEAKHAGADADTVSAALERCTELYDSRA
                     DEPIGASVIAGAVHRCCYAVIGSKDPYADLKVQDNAIAEKVVQDVQPMLSNVHDYIVA
                     AVIGNAMDYGVAGHEIAKDFTGYFRKMFEQGLAQDDTEKILALSKRVVYCTDNCGEAV
                     FDKLLCAELRKHGSHVTIVVKDQPMLNDVTMQEAKSLHLDEAADAVYHAGGGAQLGLH
                     PDLYPPEVVAAMQNATLIIAKGLANYESLTEYTGLPPIAYLMTVKCEPVARHVGAKKG
                     DLIAVLRHQ"
     gene            1637..1927
                     /locus_tag="O0X37_01965"
     CDS             1637..1927
                     /locus_tag="O0X37_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30e"
                     /protein_id="HJK61727.1"
                     /translation="MDFNLSLRRAIKTGKVVLGQNSVEKVIAENKAELVIVAKNAPAK
                     VRAIINAADNLPLYEFNGSSRQLGKECGRDHMISVLAIVDAGESDILSLKRA"
     gene            1929..2402
                     /locus_tag="O0X37_01970"
     CDS             1929..2402
                     /locus_tag="O0X37_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HJK61728.1"
                     /translation="MSEITISEDAMRMIAQFENLTGAGARDCVVDDKFGRILFVINPG
                     EMGLAIGKKGASIKKASDAFGKKVEVVEYNPDKVQFIRNCFLPVQVMTVTFEEDEDGE
                     IAYVEVRDDDRGLAIGKEGRNIIKAKKLAFRQFDVSNVELKQDEVEEEDEFDDEL"
BASE COUNT          708 a          554 c          711 g          637 t
ORIGIN      
        1 gctggtagta gcagcactga tgggcgtggc tgagactgta acgctggtga tgacagcact
       61 gcaggacccc acacagcatg cgttgtcaaa tgtgctgcag gcaatccttt tgatcatcgt
      121 aattgcaacg ctggtggata tggttgcctc ctatgtccgc gccggacggg ttctggtccg
      181 gccgattctc attgcaggaa tcacaacaat ggtccggcga ctgctggtaa gcgatctgac
      241 gtttatcgat atcatcggaa caacgattgt aattctcgga ctgaccgttg caatggtata
      301 cgttggtcgc gatgagatgg agatgatgga gcatctgaaa gatatgaagc gttcagagga
      361 tcagtaatcc tttttttgcc gatgtcaaag taaaaactaa taagggaagt acatcctata
      421 tgtatgatac ttcatccata ggatgttgta tgaggggccg tagggtagcc tggaatccta
      481 ggagactggg ggtcttctga ctcgcgttca aatcgcggcg gccccattcg tttttgtgtt
      541 taggtgataa tgttatgaga cttgcaccag aatgcagagc atgtctgctc tcaagggttg
      601 ttgcagaagc aaagcatgca ggagcggatg cagatacagt atctgccgcg cttgaacgtt
      661 gtacggagct gtatgactcc cgcgcggatg aaccgattgg cgcatcagta attgcaggcg
      721 cagtacaccg ttgctgctat gcagtgatcg gatcgaagga tccgtatgct gatctcaagg
      781 tacaggacaa cgcaattgca gagaaagttg tacaagatgt acagccgatg ctttcgaatg
      841 tgcatgacta tattgttgca gcagttattg gaaatgcaat ggattacggt gtggccggtc
      901 atgagattgc aaaagacttc accggctatt tccgcaaaat gttcgagcag ggtcttgcgc
      961 aggatgacac ggaaaaaatt cttgcgctct ccaaacgcgt ggtgtactgt accgacaact
     1021 gtggggaggc ggtatttgat aaattgctgt gtgcggaact tcgtaaacac ggcagccatg
     1081 tgacgattgt ggtaaaagat cagccgatgc tcaatgatgt aacaatgcag gaagcaaaaa
     1141 gcctgcacct ggacgaggca gcagatgcgg tgtaccacgc gggaggagga gcccaacttg
     1201 gcctgcaccc agacctgtat cctcctgagg ttgttgctgc aatgcagaat gcaaccctga
     1261 ttattgcaaa gggtctggca aactatgaaa gcttaacgga gtacacagga ctgccgccga
     1321 ttgcatacct gatgacggta aaatgcgagc ctgttgcgcg gcatgtagga gcaaagaaag
     1381 gcgatctgat tgcggtgttg agacaccagt aaaagccata ctagacacgc tatttttcag
     1441 atgtagaata cattcattat ctgcgagata caatatgtat agagtttcct cgggaagttc
     1501 agttgactga ccggataact gccgcagttg tatctttact gcgggacctg cgggaagatt
     1561 cggattttat gggggccacc tcccctggtg ccggatcgga gtgcagaact aaccacaaaa
     1621 actggtgaat ctacaaatgg atttcaactt atccctgaga agggcaatca agaccggcaa
     1681 ggtcgtttta ggacagaaca gcgttgagaa agtcattgca gaaaacaaag cagaacttgt
     1741 catcgttgca aagaatgcac cggcaaaggt ccgggcaatc attaacgcag cagacaatct
     1801 cccgctctat gagttcaacg gcagttcccg tcagctcggt aaagagtgcg gtcgcgatca
     1861 catgatcagt gttcttgcta tcgttgatgc aggcgaatcc gacattctga gcctgaagag
     1921 agcataatat gagtgaaata acaatttccg aagatgcaat gcgtatgatc gcgcagttcg
     1981 agaatctgac tggcgcaggt gcacgcgact gtgtggttga cgacaaattc ggccgaattc
     2041 tcttcgttat caacccggga gagatggggc ttgcaatcgg aaagaagggt gcaagcatta
     2101 agaaggcatc cgatgcgttt ggcaagaagg tcgaggttgt ggagtacaac cctgacaaag
     2161 ttcagtttat tcgtaactgt ttcctgccgg tgcaggttat gaccgtcacg tttgaggagg
     2221 acgaggacgg tgagatcgca tatgttgagg ttcgtgatga tgatcgcggc cttgcaatcg
     2281 gcaaagaagg aagaaatatt atcaaagcaa agaaactcgc attccgtcag tttgatgtca
     2341 gcaacgttga gctcaaacag gacgaagtag aggaagaaga cgagtttgac gacgagctgt
     2401 aaacacagct tgtttttctc tttttacaca ttcccgcaaa tgaactgctg ggtaatacac
     2461 acagaatagt ttgatttttg tgaacagata gttttcacga caaaaaaaca ggttactcct
     2521 tttcgcgccg tgatgttgcg acctgttcca gctctgtaat ccacgcatta aagtgtggac
     2581 aacatttccg catctttgca agaccgatcc
//
LOCUS       DALUAJ010000092         3892 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_15664,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000092 DALUAJ010000000
VERSION     DALUAJ010000092.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15664"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="O0X37_01975"
     CDS             <1..215
                     /locus_tag="O0X37_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HJK61729.1"
                     /translation="TILTCYGTAQKTVRRAELVGNISQISGTHSLCVVSDYRKEKKIG
                     KTLVIGEERLKHVSDGSDLLEMVTEK"
     gene            537..2198
                     /gene="thsA"
                     /locus_tag="O0X37_01980"
     CDS             537..2198
                     /gene="thsA"
                     /locus_tag="O0X37_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HJK61730.1"
                     /translation="MSAQLGGQPILILKEGGSRTRGRDAQSMNIAAAKAVAGAVRTTL
                     GPKGMDKMLVDTIGDVVITNDGVTILKEMDIEHPAAKMMVEVAKTQDDEVGDGTTSAV
                     VIAGELLKKSEELLEQDVHPTVIPLGYRQAAEKAQELLQTIAVDVKAKDKEILSKIAG
                     TAMTGKNAEASKEKLCDLIVRAITLVADADGTVDTENVKVEKRVGGAIEDSEIVEGMI
                     IDKERVHPGMPKTVKAAKILLLNAAVEYKKTEVDAEISITSPDQLQLFLDEEERMIKS
                     IVEKIKASGANVLFCQKGIDDIAQHYLSKAGILAVRRVKKSDMEKLARATGGALISSI
                     DAISADELGVAGLVEERKVGGEEMIFVEKCKNPKAVSIIIKGGTEHVVDELGRALEDA
                     LRVVGVVVEDKKIVAGGGAPEVELSLRLREYAATQGGRIQLAIEAFASALEVIPRTLA
                     ENAGLDPIDKLVELRAAHEKGEKTYGLDVFEGKPVDMLAAGVVEPLRVKTQAIASAAE
                     AAVMILRIDDVIASAKSAGPSPDEMAAMGGAGGMGGMPGGMPGMM"
     gene            2286..2834
                     /locus_tag="O0X37_01985"
     CDS             2286..2834
                     /locus_tag="O0X37_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61731.1"
                     /translation="MDPLTVLFAYGATWVDTLNYTLGAAVIALAFVYFTRNFSFVQRI
                     AAPAIVGVLVSVLGTGVVISASALPQLGYPLCLFALGSGCALSYLAVTHITNRNLGWV
                     LILVFSHLAAYCVLLSAVFVPFVGASIFGFFVAAVLFSAVLFGVATWCVLHWNPAWTP
                     WWISGDAEGRAAFFRKLRRKKE"
     gene            2907..>3892
                     /locus_tag="O0X37_01990"
     CDS             2907..>3892
                     /locus_tag="O0X37_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61732.1"
                     /translation="MRPEVLYEYDLPLEVLDEIPADEEVLEVLYARSSENYHLPILIT
                     RLRIVCAYPETSVVYRVFQIDYVDLAEVHVKFTRGLSTVLTFTRLDGEMNVFDRVRNT
                     HEEIRAALLTLRDLMIEKVGGEWQFQHRQNLLTDEYAVKESDPVLSSVPTIPAEDLFE
                     DKEMPGLGCYEPTEKLFEHFPQDAEDGDVFEESAEILPETDAELKSVAMNSRIQRMIE
                     TAEIEAEHPEITDTGEVSVNDATVLESGPGVTDSQWANEDGGDAVILPDQHAGSRQSS
                     NGTRAAVPLVPVDEDDSEVYVTPKPSPKKQKEEEAAAMIAEELDKLPVPEAK"
BASE COUNT          937 a          973 c         1063 g          919 t
ORIGIN      
        1 agacaattct cacctgctac ggtactgcac aaaagacggt ccgtcgtgcc gagctggtcg
       61 gtaacatctc gcagatttct ggaacacact cattatgtgt ggtatccgac taccgtaagg
      121 aaaagaagat tggcaagacg cttgttatcg gggaggagcg gctgaagcat gtctccgatg
      181 gttcggatct gttggagatg gtgacagaaa aataattttt ttcattcatc tgattttttg
      241 agccgctgtt tgttaatgcg ctgtctggtt gatcccatac tgataatatt gagtcccgca
      301 gatttctctg tcatccctcc agaaaagcag tcagtgggag gatcattcat atctgataaa
      361 caagacatct ccttactcaa caggcgcgcc cctgcggggt tgtgctgggc ttggtaaatt
      421 ttcttacatt tttgtcccga ttgattcaca atacaatcaa atttatatcg atcaacaaat
      481 caacctaatc tgcatacatt actctgtatg ccacagagta tttggagata gaaatcatgt
      541 cagcacaact tggcggacag cctattctga ttcttaaaga aggcggttcc cgtacccgtg
      601 gacgggatgc ccagagcatg aacattgctg cagcaaaggc agttgcaggt gcagtcagaa
      661 ctacccttgg tccgaagggc atggacaaga tgctcgtcga caccatcggc gatgttgtca
      721 tcaccaacga tggtgttacc atcctcaaag agatggatat tgagcacccg gcagcaaaga
      781 tgatggttga ggtcgcaaag acccaggacg acgaagtcgg agacggaacc acctctgccg
      841 tcgttatcgc aggcgaactc ctgaagaaat ccgaagaact tctcgagcag gatgtccacc
      901 cgactgttat cccccttgga taccgccagg cagcagagaa ggcacaggaa cttctccaga
      961 ccattgcagt cgatgtaaag gcaaaggaca aggaaatcct ctccaagatt gcaggtactg
     1021 caatgaccgg caagaacgcc gaggcatcca aagagaaact ctgcgactta atcgtccgcg
     1081 caatcaccct cgttgctgac gcagacggaa ccgttgacac ggagaacgtc aaagtcgaga
     1141 agcgtgtcgg tggtgcaatc gaggactccg agattgtcga aggtatgatc atcgacaaag
     1201 aacgtgtcca ccccggtatg ccgaagaccg ttaaagctgc aaagattctc ctcctgaacg
     1261 cagctgtcga gtacaagaag accgaagttg acgcagagat cagcatcacc agtcccgacc
     1321 agctccagct gttccttgat gaggaagagc gcatgattaa gagcatcgtc gagaagatca
     1381 aggcatccgg tgcaaacgtt ctcttctgtc agaagggtat cgacgacatc gcccagcact
     1441 acctctccaa ggcaggcatc ctcgcagtcc gccgtgtcaa gaagtccgac atggagaagc
     1501 tggcacgtgc aaccggcggc gcactgatct cctccattga cgcaatcagc gcagatgagc
     1561 ttggtgtcgc tggtcttgtc gaagagcgca aggtcggcgg agaagagatg atcttcgttg
     1621 agaagtgcaa gaacccgaag gcagtctcca tcatcatcaa aggcggaacc gagcacgtgg
     1681 ttgacgagct tggccgtgca cttgaggacg cactccgtgt tgtcggtgtt gttgttgagg
     1741 acaagaagat tgttgcaggc ggaggagcac cggaagtcga gctctccctc agactccgtg
     1801 agtacgcagc aacccagggc ggccgtatcc agctcgcaat cgaggcattt gcatccgcac
     1861 ttgaggtcat cccgagaact cttgcagaga atgcaggtct tgacccaatc gacaaacttg
     1921 tcgagcttcg tgcagcccat gagaagggcg agaagaccta cggtcttgac gtcttcgagg
     1981 gtaagccggt tgacatgctt gcagcaggcg ttgttgagcc gctccgtgta aagacacagg
     2041 caattgcatc cgcagcagag gcagctgtca tgattctcag aatcgatgat gtgattgcat
     2101 ccgcaaagtc cgcaggcccg tctccggacg agatggcagc aatgggcggt gcaggcggca
     2161 tgggcggtat gcccggcggc atgcccggta tgatgtaaat ctccccaata tcaatctctt
     2221 ttttgaaacg ttctctttct ccctgaaacg ctatattctc ccaaagcaaa cacatgtacc
     2281 agtttatgga tccgctgacc gttcttttcg catatggtgc cacttgggtg gatactctca
     2341 actacacact tggtgcggcg gtgatcgccc tggcatttgt gtactttacc cgaaattttt
     2401 ccttcgttca gcggattgct gcccctgcga ttgtcggggt ccttgtgtcg gttcttggaa
     2461 ccggtgttgt aatctctgca tccgccctgc cgcagcttgg gtatcctctg tgtctgtttg
     2521 cgctcggaag cggctgtgcg ctcagctatc ttgcggtgac ccacatcaca aatcgtaatc
     2581 tcggatgggt gctgattctt gtcttctcgc accttgctgc gtactgtgta ctgctttccg
     2641 cggtgtttgt tccgtttgtc ggtgcatcca tcttcgggtt ttttgttgca gcggtactct
     2701 ttagtgcagt tcttttcgga gttgccacat ggtgtgttct gcactggaat cctgcatgga
     2761 cgccgtggtg gatatctggg gatgcggagg gccgggctgc gtttttccgg aaacttcgca
     2821 ggaagaagga gtaactgtct gcgtcctgtt tacgaacttt aactaagaaa taacatcaaa
     2881 ttataacaac agttgagacg gtaactgtgc gacctgaagt cctctatgaa tacgatctcc
     2941 cgcttgaagt actggatgag attccggctg atgaagaggt gctggaggtt ctctacgccc
     3001 gcagtagtga aaactatcat ctgcctatct taattacacg cctgcggatt gtctgcgcat
     3061 atccggaaac atcggtggtg taccgtgtgt tccagattga ttacgtggat ctggcagagg
     3121 ttcatgtgaa gtttacccga ggtctttcca cggttctgac cttcacccgt cttgacggcg
     3181 agatgaacgt cttcgaccgc gttcgcaaca cccatgagga gatccgggca gcccttctga
     3241 ctctgcgcga tctgatgatc gaaaaggtcg gcggtgaatg gcagttccaa caccggcaga
     3301 acctgctgac ggatgagtat gcagtaaagg aaagcgatcc tgttttgtca tcggtcccaa
     3361 caattcctgc agaggatctc ttcgaggaca aagagatgcc gggccttgga tgctacgaac
     3421 caacggagaa actgttcgag catttcccgc aggatgctga ggacggagat gtgtttgagg
     3481 agtccgcaga gattcttcca gagactgatg ctgagctcaa atctgttgcg atgaactcgc
     3541 ggattcagcg gatgattgaa actgcagaga tcgaggcaga acatcctgag attacagata
     3601 cgggggaggt atccgtcaac gacgcaacgg ttcttgagag tggtcccggt gtcaccgatt
     3661 cccagtgggc aaacgaggat ggaggcgatg cggtgattct gccggatcag cacgctggtt
     3721 cccgccaatc cagtaatggt acgcgggctg ccgtaccgct ggtaccggtt gacgaggatg
     3781 attccgaggt gtacgttacc ccgaaacctt ccccgaagaa gcaaaaggag gaagaggcag
     3841 cagcaatgat cgctgaggaa cttgacaaac tcccggttcc ggaggcaaaa ga
//
LOCUS       DALUAJ010000093         1699 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_15720,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000093 DALUAJ010000000
VERSION     DALUAJ010000093.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15720"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(38..499)
                     /locus_tag="O0X37_01995"
     CDS             complement(38..499)
                     /locus_tag="O0X37_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61733.1"
                     /translation="MSEKIIDNEEKVPVSGTNISQSTPVLSPANPETELLKKLQESID
                     AMNALIQEVRETNVTSSKKTETANRLSCAVYILTAGLFLLTIINIGVVVAASVLSAGS
                     AELINASMNLLDLAIFVGMLFVLLAAIFFIINWVPDLCEWFKERKMRKSSP"
     gene            complement(568..927)
                     /locus_tag="O0X37_02000"
     CDS             complement(568..927)
                     /locus_tag="O0X37_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61734.1"
                     /translation="MTTTDEFSEFDYPYPILLVCMKPGYTDEDGIRVPARIVSSTAVA
                     GHLQDVSAREILRAPEGIYLPGDRRFYTSTPLAEDSLLKITESNGDVTSWTIVGCESA
                     AHTLFFPVRYTYLLRRQ"
     gene            complement(914..1276)
                     /locus_tag="O0X37_02005"
     CDS             complement(914..1276)
                     /locus_tag="O0X37_02005"
                     /inference="COORDINATES: protein motif:HMM:NF024659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HJK61735.1"
                     /translation="MPEATVDQVKQLLGPAAVRYTDEEISLSLEEAKEFMAMKGVGSA
                     AMPRASYGKAQRLLAARSLLAESPAAANVASRSEGNMSITYRDSDSRISIWDAEIGEL
                     LRLLRPSSSPVIPLYDNY"
     gene            complement(1279..1515)
                     /locus_tag="O0X37_02010"
     CDS             complement(1279..1515)
                     /locus_tag="O0X37_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61736.1"
                     /translation="MMQAQVTDGTLTLKDASGKYVTFRKGDWITVPDTSGEWPAAVRE
                     AVRTGQLAVAAVADLPPQKVKAVPEKKPGKAVEE"
BASE COUNT          424 a          483 c          395 g          397 t
ORIGIN      
        1 gagcgatatt agaatagcga cgggggaaaa gagggggtta gggggaagat tttctcatct
       61 tgcgttcttt gaaccattca catagatcag gaacccaatt aatgataaag aagatggcag
      121 ccagtaatac aaacagcata cctacaaaaa tcgccaaatc taaaagattc attgaagcat
      181 taattaactc agcagatccg gcagataaaa ccgaagcggc cacaacaaca ccaatattga
      241 tgatggtcag taagaacaag cctgccgtca atatgtaaac cgcacacgag agcctgttgg
      301 ctgtttctgt cttttttgac gaagttacgt tagtttctcg aacctcctga ataagcgcat
      361 tcatggcatc aattgactcc tgcagctttt tcaataactc agtttctgga tttgcaggtg
      421 aaagaactgg tgttgattga gaaatgtttg ttccagagac aggcactttt tcttcattat
      481 caatgatttt ttctgacatt acggatcaat aaccaatgta tccacatata aatataccca
      541 tcatctttga accatgatga gggagactta ctgccgtctc agcagatacg tgtaccgcac
      601 cggaaaaaag agcgtatggg ccgctgactc gcagcccaca atggtccagg aagtaacatc
      661 cccgttggat tcggtaattt tcagaagaga atcttcggca agtggagtag aggtgtagaa
      721 ccgccggtcg cccggaaggt agatgccctc cggtgcccgc aggatctccc tggcagagac
      781 gtcctgcaga tgaccggcaa cagctgtact actgacaatc cgggccggta cccgtattcc
      841 gtcttcgtca gtataacccg gcttcataca cacaaggagg atcggatagg ggtaatcgaa
      901 ctcggagaac tcgtcagtag ttgtcataga gtggtatcac tggtgaggaa cttggtctga
      961 ggagccgcag cagctccccg atctcagcat cccagatgga aattctgctg tcactgtctc
     1021 ggtaggtaat gctcatgttc ccctcgcttc tggatgcaac attcgctgcc gcaggactct
     1081 ctgccagcag cgaccttgca gcaagaagac gctgagcttt cccataactg gctcggggca
     1141 tcgccgcact cccgacaccc ttcatcgcca tgaactcttt tgcctcttcc agggacaggc
     1201 tgatctcctc atccgtatac cggaccgcag caggaccaag cagctgcttc acctgatcaa
     1261 ccgtcgcctc gggcattctt actcctcaac cgcttttccc ggcttcttct ccggcaccgc
     1321 tttcaccttc tgcggcggca gatccgcaac cgcagcaacc gccagctgcc cggtccggac
     1381 cgcctcacgc acagccgcag gccactcacc ggaagtatcc ggaaccgtga tccagtcccc
     1441 tttgcggaac gtcacatact tccctgatgc atccttcagt gtcagcgtcc cgtccgtaac
     1501 ctgtgcctgc atcatctcac cccagcagcc ccgtcagttt cgtaatcagt ttcggatgag
     1561 aaatgtaggc attcgccttc gccagagcca ccgtcttata ggcgaccata tccttcccca
     1621 ggaacttgcc gtccacactg taggtccgga tcggatgacg tacatcccag gtaccgcagc
     1681 tcagcgtccg cagcacata
//
LOCUS       DALUAJ010000094         1563 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_16014,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000094 DALUAJ010000000
VERSION     DALUAJ010000094.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16014"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(135..698)
                     /gene="dcd"
                     /locus_tag="O0X37_02015"
     CDS             complement(135..698)
                     /gene="dcd"
                     /locus_tag="O0X37_02015"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HJK61737.1"
                     /translation="MILVDWEIADHIKRGFIGVDPYDPALVQPNSLDIRLGNHFVWYE
                     PCNDVIDPYKKETILSHTSERKSSYFDIMPGQFVLAETLETITLPDNIVSSIEGKSSV
                     ARLGIELHQTGGWIDAGFSGTITLEMCNVNCRPVRVYAGMPIGQLVFYVTKRCSCPYD
                     KKPDAKYQNQKNATISRYDKNTLKNVE"
     gene            complement(795..1340)
                     /locus_tag="O0X37_02020"
     CDS             complement(795..1340)
                     /locus_tag="O0X37_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HJK61738.1"
                     /translation="MIRLSTPLGAEVLDLQAGDRVLLSGTIYTARDEAHLKIQKDGFP
                     FDPAGAVLYHCGPVVDTKRNVIVAAGPTTSARMNGLTAPMIDAGVRALIGKGGMSAEV
                     REILSGRGVYFAFTGGCAALAASCMGLVACHYPELGMAEAVWEIRVRDMPLVVGIDAH
                     GGDLFADVACEVRERYTKLPL"
     gene            complement(1337..>1563)
                     /locus_tag="O0X37_02025"
     CDS             complement(1337..>1563)
                     /locus_tag="O0X37_02025"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HJK61739.1"
                     /translation="AKEAILEPIDTMDAYEQKICDAINSLGIGPMGLGGDTTALAVKV
                     KRGFCHTASLPVAVNIQCWCCRRGVREVAL"
BASE COUNT          408 a          417 c          364 g          374 t
ORIGIN      
        1 ctgtcatatc cagttatatt gatcggggca ggttatgagg gttgggaatg tgaaagacag
       61 catactgaat gtggatttca tctggggatg agacggcacc tactggcaat cagaaaaagt
      121 agattggggg agatctattc aacgtttttg agagtattct tgtcgtagcg cgagatggtt
      181 gcatttttct ggttctgata ctttgcatcg ggttttttgt catagggaca ggaacagcgt
      241 ttggttacat agaagacgag ctgacctatg ggcatccctg catagacacg gaccggacgg
      301 cagttaacat tgcacatttc aagagtaatt gtcccggaaa atccggcatc gatccagccg
      361 ccggtctgat ggagttcaat accgagacgt gcgacactgc tttttccttc aatggaggat
      421 acaatgttgt cggggagggt gatggtttca agggtttctg caaggacaaa ctgtcctggc
      481 atgatgtcaa aataggaact ttttcgctcg ctcgtgtgag agagaatggt ctcttttttg
      541 taaggatcaa tcacgtcgtt acagggttcg taccagacaa agtgatttcc aagacggata
      601 tccagtgagt tcggctgtac tagcgcagga tcatacgggt ccacaccgat aaatccgcgt
      661 ttgatgtgat ccgcgatttc ccaatctacc agaatcatat cttatctata tgaaggcaac
      721 agtaataaac cctgtatgaa tttgtgggat tgtcccattg agtttacaca gatatttacc
      781 agctttgaaa aatattagag aggtaatttt gtatatcgtt cacgaacctc acacgcgaca
      841 tccgcgaaca gatcgccgcc gtgggcatca atgcccacca ccagcggcat atcccgtaca
      901 cggatttccc acaccgcctc agccataccc agctcagggt aatgacacgc aacaagtccc
      961 atgcatgatg cagcaagagc agcacaacca ccggtaaacg caaagtacac accccgtcct
     1021 gacagtatct cacgtacttc ggcagacatt ccgccttttc caatcagagc cctcaccccg
     1081 gcatcgatca tcggcgcagt cagaccattc atccgtgccg acgtcgtcgg acctgccgca
     1141 acgatcacat ttcgcttggt atccaccacc ggtccgcagt gatacaacac ggcccctgca
     1201 ggatcaaacg ggaaaccatc cttctgaatc ttcaaatggg cctcatcccg tgcggtgtaa
     1261 atcgttccgg acagaagcac gcgatcccct gcctgcagat ccaaaacctc agcacccagc
     1321 ggagtactca gacgaatcac agtgccacct cccgaacccc gcgcctgcag caccagcact
     1381 ggatattcac cgcaaccggc agagaggcag tatgacagaa tccgcgcttg acctttaccg
     1441 caagtgctgt cgtatcacct ccaagaccca taggacctat cccaagtgaa ttaatcgcat
     1501 cacaaatctt ctgctcatat gcatccattg tgtcgatcgg ttccagaatc gcctcctttg
     1561 caa
//
LOCUS       DALUAJ010000095         3586 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_16022,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000095 DALUAJ010000000
VERSION     DALUAJ010000095.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16022"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(308..766)
                     /locus_tag="O0X37_02030"
     CDS             complement(308..766)
                     /locus_tag="O0X37_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HJK61740.1"
                     /translation="MQQITIHLGATGITPTSTFSGGNISPKIDLTGVPAEVKYIAVIV
                     QNKSGEGKTHCIWSVWNIPSVGYIPPGYEQGPAPSFPFPAVQGLNDFGEHGWHGPESK
                     LGSQERMLFQVFGRAEPLSISADAKMDEVIEALRDGKTTAYGSLESTYFL"
     gene            871..1737
                     /gene="tsaA"
                     /locus_tag="O0X37_02035"
     CDS             871..1737
                     /gene="tsaA"
                     /locus_tag="O0X37_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HJK61741.1"
                     /translation="MDELTIYTDGASRGNPGEAAAAWLILRGCEVLESEVRVLGVQTN
                     STAEYTALLGALTAAKKYGDPAATSLTVYSDSELMISQMTGTYAVRSETLKPLYTEAA
                     AAASVYAKVRYVHVPRENAYVGSCDWLCNNALDTLAAAKYAAEQQKRHGPIECRPIGI
                     VHSPFLDRKSAPNQGKNTNELSTVEVYPEYADGLLGLSEGDSVFILCWFDRSDRSILQ
                     VIPRGGRRGLTGVFATRAPVRPNPISLTLVTIVSIDKTRITVKGLEALDQTPVLDIKP
                     YYGDIDVPAKQD"
     gene            1840..2706
                     /locus_tag="O0X37_02040"
     CDS             1840..2706
                     /locus_tag="O0X37_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HJK61742.1"
                     /translation="MSDKPEGCDGNCDGCTQKTDTCQQPKKADISVRHVILVLSGKGG
                     VGKSTVSVNLAYALSNHGYQTGLLDLDIHGPSIAKMLGIEDLKLQAIGNKIMPVKVPG
                     SLKVVSMALLLNTTDSPIVWRGPMKAAAIQQFLGDVDWGDLDYLVVDLPPGTGDEALN
                     IVQFAPNVEGAVVVTTPQDVAVLDSTKAIKFVEMMDLPVLGIIENMSGMVCPHCGEVV
                     DIFGKGGGEKAAKQYEVPYLGAIPIDIEMRKAGDEGRPFIVRKPGQANPTWDAVDKVM
                     ENLIAEVEKRDE"
     gene            2956..>3586
                     /locus_tag="O0X37_02045"
     CDS             2956..>3586
                     /locus_tag="O0X37_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61743.1"
                     /translation="MSERRTLTIGVAINVGNYESLRLEVSDCVQTEEEAAELAAYLNR
                     VLDGYAQNDATTRAAIDRYRSRVLEKYVPEEEPSSSDTLDEIYGTAPVSEDISFSTGP
                     EPSEPVSAPEVLTPVVSEPGPVVVVDTPSVPAPVSAVDVPSVSDPVPSEQEPVAAADT
                     PSVPEPVSFESEPVPAVETPSVPDPTPSESEPAAAAQAPKTSEPVTYTCE"
BASE COUNT          854 a          971 c          932 g          829 t
ORIGIN      
        1 tcttcttgga gtgctttcca gctgccttcg ttgcttcgac tttttcttct gcaaccggtg
       61 ctgctgtctt ttttgccgct ttgtgtgaca tacattttca tctgcttttg gggtgtataa
      121 agctgtgtgt cagggtaatg aaaaatctca ttatttcttc ctgttttgaa aataccttga
      181 aatctggcga taagtcagtc ctgtaaaaca cttggatttc tcctcgcgca caaggccggt
      241 acaaccgcgg cgatctatca ggattttttt gcttcaaact gcggcacgcc gcaaaaaaga
      301 acggggttta gaggaagtag gttgactcaa gacttccgta ggcagtggtt tttccgtcac
      361 ggagagcctc aataacctca tccatttttg catccgcaga gattgacaac ggctcggcac
      421 ggccgaagac ctgaaacagc atccgctcct gactgccaag ctttgactcc ggtccgtgcc
      481 agccgtgctc gccaaagtca ttgaggccct ggaccgcagg gaaggggaag gaaggcgcag
      541 gcccctgctc atacccaggc ggaatgtacc ctaccgaagg aatattccag accgaccaga
      601 tacagtgtgt ctttccttct ccggacttgt tctgaacaat cactgcgatg tactttacct
      661 cagcgggaac gccggtcaga tcgatctttg gcgagatgtt tccgccggag aacgtacttg
      721 tcggcgtaat cccggtcgcc ccgagatgaa tggttatctg ctgcatacag tacactcacc
      781 cgagaaccta atagatgtat agattgggga aaaaatctcc cgcgtcccaa atccccagta
      841 ataaatagca gaaaaagcaa cacaacccgt atggacgaac tgaccatcta caccgacggt
      901 gcaagccgcg gaaatccggg ggaagcagcc gcagcatggc tgattctccg gggatgcgag
      961 gtgctggagt ctgaggtgcg ggttcttggg gtgcagacca acagcaccgc tgagtacacg
     1021 gcacttctgg gtgctcttac cgctgcaaaa aagtatggtg atccggctgc cacttccctc
     1081 accgtctact cggacagtga actgatgatc tcgcagatga caggaacata tgcggtccgc
     1141 tccgagaccc tgaagcccct ttacaccgaa gcggcagctg cggcgtccgt ttatgcaaag
     1201 gtacggtatg tacatgttcc gcgcgaaaat gcatacgtcg gatcctgtga ctggctgtgc
     1261 aacaatgcgc ttgacactct tgccgcagca aagtatgccg cagaacagca gaagcgtcat
     1321 ggtcctattg agtgcaggcc gattggtatc gtccactcgc ctttccttga tcggaaaagt
     1381 gctccgaatc aggggaagaa cacaaacgag ctgagtaccg ttgaagtgta ccccgagtat
     1441 gctgacgggc ttttggggct gtctgaaggt gactcggtct tcattctctg ctggtttgat
     1501 cgctccgacc ggagtattct tcaggtcatc ccccgcggcg gccgccgggg tctgaccggc
     1561 gtgtttgcaa cccgtgctcc ggtcagacca aacccgatct ccctgacgct ggtaacaatc
     1621 gtctccattg acaaaacccg catcacggtt aaagggcttg aagcactcga tcagacacca
     1681 gtgctggaca tcaaacccta ctacggcgac atcgatgtcc cggcaaaaca ggactgaggc
     1741 gctgccactc tcccgtgcca aatacgtaaa ggatatactc tcccccgtac aacagattat
     1801 gcaataagtt atatagactt acattacagg tgaaatcaaa tgtcagacaa acccgaagga
     1861 tgcgacggta actgcgacgg atgcacgcag aagaccgata cctgccagca gcccaagaag
     1921 gcggatatca gcgtacgcca tgtgattctt gttctctccg gaaaaggcgg cgtcggcaag
     1981 agtacggtct ccgtcaacct tgcgtatgcc ctctcgaacc acggatatca gaccggtctt
     2041 ctcgatctgg atattcatgg tccaagcatt gcaaagatgc ttggaattga ggatctgaaa
     2101 ctgcaggcaa tcggcaacaa gatcatgccg gtcaaggtcc ccggctcact caaggttgtc
     2161 tcaatggccc ttcttctcaa caccaccgac agtccgatcg tctggcgcgg accaatgaag
     2221 gcagcagcaa tccagcagtt cctgggcgat gttgactggg gagatcttga ctacctcgtt
     2281 gttgatctgc cgccgggaac cggtgacgag gcactgaaca ttgtacagtt tgccccgaat
     2341 gtagagggag ctgttgttgt taccactccg caggatgttg ctgtgcttga ctccacaaag
     2401 gcaatcaagt tcgtggagat gatggacctg ccggttctcg gtattatcga gaacatgtcc
     2461 ggtatggtct gcccgcactg cggtgaggtt gtggacatct tcggcaaggg cggaggagaa
     2521 aaggcagcaa aacagtacga agtcccctat cttggtgcaa ttccaatcga cattgagatg
     2581 cgcaaggcag gcgatgaggg cagaccgttc atcgtcagaa aaccgggaca ggcaaacccg
     2641 acctgggatg cggttgacaa ggttatggaa aacctgatcg ccgaagtcga gaagagagac
     2701 gaataatcct ctcttttttt gttgtttcca ttatctgtcc ggcaggtatt ttacaggaat
     2761 atactgattg taaaacagac agagggcatc agacccctgc gtcatcatac cctttcgggt
     2821 cagcgggggt aactttcatc atctgccgcc tgtgtggaat acctattcta ttgaaaagcc
     2881 aaactatcag tctttcgctt tagagcatcc ggatacacat cattaattct gcaaaacacc
     2941 aacactatta ctattatgtc agagaggagg acactgacaa tcggggttgc aataaacgtc
     3001 ggcaactatg aaagtctccg gcttgaggtg agtgactgcg tgcagaccga ggaggaagct
     3061 gcagaacttg ccgcatatct gaaccgggtt ctggacgggt acgcccagaa tgatgcgaca
     3121 acccgtgcag cgattgaccg gtaccgctcc cgcgtactgg aaaagtatgt tcctgaggag
     3181 gagccatctt cgtctgatac gcttgacgag atctacggca cggctccagt atcagaggat
     3241 atctcctttt caacagggcc ggaaccgtct gaaccggtgt ctgctccaga agttcttacg
     3301 cctgtcgttt ccgagccggg tcccgtggtc gtcgtagata ctccgtcagt acctgcaccg
     3361 gtatctgctg tggatgttcc ctcggtttcc gacccggttc cctccgagca ggaaccagtt
     3421 gccgctgccg acactccatc cgttcctgag ccagtttctt tcgaatctga accggttcct
     3481 gctgtggaaa ctccatccgt tcccgatcca accccctccg aatccgaacc ggctgccgca
     3541 gcgcaggctc cgaagacctc cgagccggtt acctacacct gtgaaa
//
LOCUS       DALUAJ010000096         2053 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_17025,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000096 DALUAJ010000000
VERSION     DALUAJ010000096.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17025"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="O0X37_02050"
     CDS             complement(<1..282)
                     /locus_tag="O0X37_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HJK61744.1"
                     /translation="MAGHFPEKAVHGGKVQELRRVFGHDFLDVSASMNPFVPQVSADF
                     SCADLSVYPDDSYAELKECIARVFSRDPEEICLGNGSAELIRVYCHTMLR"
     gene            500..685
                     /locus_tag="O0X37_02055"
     CDS             500..685
                     /locus_tag="O0X37_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HJK61745.1"
                     /translation="MDRITIRLPPQQVDLLEKLVETGEFPTVSEAVRYAVREFLAGRS
                     ERILRESEQVSTFDVRL"
     gene            712..1875
                     /gene="ftsZ"
                     /locus_tag="O0X37_02060"
     CDS             712..1875
                     /gene="ftsZ"
                     /locus_tag="O0X37_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HJK61746.1"
                     /translation="MQSIINEALKNSELEKGMQKTSIVDDDDMLGQPRIVIVGCGGAG
                     NNTINRLHNMRVAGSETIAINTDKQHLDMIQADKRVLIGKSLTRGLGAGGFPDVGRRA
                     AEMARPTLEEILKDADLVFVTAGMGGGTGTGSAPVVAQIAKEHGAIVVAMVSYPFQVE
                     RARMLKAEDGLEAMRQAADSVIVLDNNRLKNFVPNLPLGQAFSVMDQIIAETVKGISE
                     TITEPSLINIDYADVRAIMSKGGLAVMLVGESKQQNKAESVIRDCLAHPLLDIDFRGA
                     TGGLIHITGGNDLTLHDAEEIAQQLTYELDPHADVIWGARVRKDFEGKASVMAIMTGI
                     QSPQILGGHAVNTATRSSRSEGSIYGFQSSSPANQSSNNTATARSGSSFDWIM"
BASE COUNT          563 a          602 c          506 g          382 t
ORIGIN      
        1 gcgaagcatg gtatgacagt agacgcggat cagttcggcg gatccgtttc ccaggcagat
       61 ttcctcagga tcccgcgaaa acacccgtgc aatgcactct tttaactcgg cataactgtc
      121 gtccggatac acggacaggt ccgcgcagga gaagtccgcc gaaacctgcg gcacaaacgg
      181 gttcatgctt gcgctcacgt caagaaagtc atgaccgaaa acacgcctga gctcctgcac
      241 cttacccccg tgaactgcct tttccggaaa atgtcctgcc atactgcctt cttcctgata
      301 cctactattc acaccccgga cataaataaa gtgcagaaag gaaagatttc ccggttaacc
      361 cgaactgcag agtatacata tctatatata ccaagagatg caattatgat ttgtccaaaa
      421 ttaggacgtc agtggagtgt gttacaattg tcagacaagt gtcggacagg tggctgacaa
      481 ttgtctgaat ggagttacaa tggacagaat caccatccgc ttaccgccgc agcaggtaga
      541 tctgctcgag aagctcgtgg aaacagggga gtttcccacc gtatcggaag ccgtcagata
      601 tgcagtaaga gagtttctcg caggaagatc agagcggatt cttcgtgaaa gtgaacaggt
      661 atccacattc gacgtacgac tctaaagtta aaattcattg gggtgtgaaa aatgcagagc
      721 atcattaacg aggcattaaa aaattcagaa cttgagaagg gaatgcagaa gacctccatc
      781 gttgacgacg acgacatgct cggacagccg agaattgtca ttgtcggatg cggcggtgcc
      841 ggaaacaaca ccatcaaccg tctccacaac atgagagtgg caggatccga gacgatcgcc
      901 atcaacaccg acaaacagca ccttgacatg atccaggccg acaagcgtgt tctcatcggc
      961 aaatccctga cccgcggatt aggtgccgga ggattcccgg atgtcggccg ccgtgcagcc
     1021 gagatggccc gcccgaccct tgaagagatt ctgaaagatg ccgacctcgt gttcgttacc
     1081 gccggtatgg gcggaggaac cggaaccgga tccgcaccgg tcgttgccca gattgcaaaa
     1141 gagcacggcg caatcgttgt cgccatggtc agctatccgt tccaggttga gcgtgcaaga
     1201 atgctcaaag cagaagacgg tctggaagca atgcgccagg cagccgactc cgtcattgtc
     1261 ctggacaaca acagactcaa gaacttcgtc ccgaacctcc cgctcggcca ggccttctct
     1321 gtgatggacc agatcatcgc agaaaccgtc aaaggcatct ccgagaccat caccgaacct
     1381 tccttgatca acatcgacta cgctgacgtc cgcgccatca tgagcaaggg cggccttgcc
     1441 gtcatgcttg tcggcgagtc caaacagcag aacaaagcag agagcgttat ccgcgactgt
     1501 ctcgcacacc cgcttcttga catcgacttc agaggcgcaa ccggcggtct gatccacatc
     1561 accggcggca acgacttaac gctccacgac gcagaagaga ttgcacagca gctcacctac
     1621 gagcttgacc cgcacgcaga tgtcatctgg ggtgcacgcg tccgcaagga cttcgaaggc
     1681 aaggcctctg tcatggcaat catgaccggt atccagagcc cgcagatcct tggcggccac
     1741 gcagtaaaca ccgcaaccag atcctcccgc tcagagggaa gcatctacgg attccagtcc
     1801 tcctcaccag ccaaccagtc ctcaaacaac accgcaaccg cccgcagcgg ttcgtccttc
     1861 gactggatca tgtaaggctg ccagcccata gttacaccgg tttcatacat tacaccacac
     1921 acaggttggt ccgggacaaa accacaccac actacaccac accaaaactc ccggaaccac
     1981 ccctcaggag acaaagaacg gacaatacaa atacaagatc aatactcaca cggggacgga
     2041 cacagggcac aga
//
LOCUS       DALUAJ010000097         4973 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_17037,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000097 DALUAJ010000000
VERSION     DALUAJ010000097.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4973)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4973)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4973
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17037"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            150..431
                     /locus_tag="O0X37_02065"
     CDS             150..431
                     /locus_tag="O0X37_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61747.1"
                     /translation="MDMQQDGPRPFSPITVTDEVIAYMDKLGGDFRISTSCSGPVLMP
                     VKIKPAKQSDIPIKAGSHTIYVSQYQLPWLTEINMRLVPRILYTENDDV"
     gene            421..834
                     /locus_tag="O0X37_02070"
     CDS             421..834
                     /locus_tag="O0X37_02070"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HJK61748.1"
                     /translation="MTFEELEHTADVRMKITAPTLPELFAESGFALAETLYGEYQREP
                     AAVSFPIESEGKDREEGIVNFLSELLFLTETEYLVPVSFDLCVEGTAVRGTVAGVLFD
                     RAKHAGGIGVKGISYSGLSFRETAAGYELIIIFDI"
     gene            838..2280
                     /locus_tag="O0X37_02075"
     CDS             838..2280
                     /locus_tag="O0X37_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HJK61749.1"
                     /translation="MNDQIRNISDTEWEIPRDFVPGMRVPGTFFLSHHLAEGLEEGAL
                     TQLANVATLPGILRCSLGMPDIHWGYGFPIGGVAAFDEDEGIISPGGVGFDINCGVRM
                     ITTPLTASDIPDMKGLIEDLFAAVPTGVGSKSPVRLSQNDLSDILTDGAANAVEMGYG
                     VEEDVRRCEENGAMAGAKLEFVSKKARARGIPQCGTLGSGNHFLEVQAIDEIADPKTA
                     EIFGVKEGQICVMIHCGSRGLGHQVCTDHLKVLEEAVKKYNISLPDRQLACAPLHSPE
                     GEAYFGAMAASANYAWANRQIITHLVRELLEKKFGIAYEEMPLVYDVAHNVAKWEEHE
                     IPGDGKGRRRVCVHRKGATRAFGPGRNEIVQEFRDAGQPVIIPGSMGTSSYLLAGTTT
                     AMERTFGSTCHGAGRVQSRSSAKRSLTGTAVAEDLARRGIIVRAPNAAAIAEEAPDVY
                     KPSSEVVQVVHDAGISRIVARFRPLGVIKG"
     gene            2277..2870
                     /locus_tag="O0X37_02080"
     CDS             2277..2870
                     /locus_tag="O0X37_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61750.1"
                     /translation="MMDTHNHIAVFWDNKMMFHRLVEDACGYCEAVTPLMLAAPFYKG
                     RYTGIIIPTGFGNLQYSKLLPALRAVSGRIEDYLENGGKLLVYGAADPANTGNPYNWL
                     PVDVEYHFEFAEHKLEIDASSPWKSLFDGYDTDKFAADGWFVKYPGKPVAVAENGFPV
                     LVECPVGEGTLLLASAHEYPSDAFLKQFGAAATEIQF"
     gene            2962..3948
                     /locus_tag="O0X37_02085"
     CDS             2962..3948
                     /locus_tag="O0X37_02085"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HJK61751.1"
                     /translation="MTQTIFDVLRILDEAGIGNPVPGEVISERLGITRTAVWKYVNLL
                     REVGYCIDASPKTGYSLQKRTWLLLPYEVKRFLKTQTIGQEMHHFEQVPSTNALARQM
                     LHEVGNEVQPGTVLIAEQQTGGLGRMNRVWMSPEGGIWATIVVMPKLNVDQTFSIMMA
                     GSLALARAIRHEFGLSALIKWPNDVFIGDKKVAGMTIELAADEDILRYCLIGVGIDVN
                     VSVERTMPNISKLVTSISDELGHEVERAQFFAKFLKEFERRYQMVLMGETEPLFREWR
                     SLSYTLHRRVRIRTMKESFEGEAIDIDEHGALLVHRDGGEVERVIAGDCFML"
     gene            4074..4628
                     /locus_tag="O0X37_02090"
     CDS             4074..4628
                     /locus_tag="O0X37_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HJK61752.1"
                     /translation="MYGNEKRARLLAESAAFIALIAVGGWISIPILIIPFTLQNIFIL
                     LAAAVMKRYAVIPVGLYVLFGSLGLPIFHNGTAGLGVLLGPTGGFLIGFVIMGAVAGW
                     LFEKETIRYDILACAAFVTGLYVFGTAWFMISTGAELTAALLACAVPYIPGDIIKCVI
                     VEAVILRLRSAKNKTSQTNNDIHS"
     gene            4645..>4973
                     /locus_tag="O0X37_02095"
     CDS             4645..>4973
                     /locus_tag="O0X37_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJK61753.1"
                     /translation="MDIPHLSIPEGISAVTGDNGAGKTTLLRLAAGMLLPETGTITIN
                     GTAPRACEVGCVSEFPDRHLIFSVVRDEIASPLRFAHRKPAEIDDAVTSITDRLGIAH
                     LLDRECRT"
BASE COUNT         1221 a         1232 c         1371 g         1149 t
ORIGIN      
        1 ggaattctct ctacacgccg caacccctcc ggaactctcg gggtatacga agtcaaagaa
       61 cagtatgttg atgtaattga gcaggcgatc gcaacagcgg tctagccctc ccttaacttt
      121 tttgacgggc aaggcccaat taatacagaa tggacatgca acaggacggt ccccgtccct
      181 tttctcccat caccgtcact gatgaagtga tcgcctatat ggacaaactc ggcggagatt
      241 tccggatcag tacctcgtgc agcggcccgg ttctgatgcc ggtcaagatc aaaccggcga
      301 aacaaagcga tatcccgatc aaagcaggca gccatacaat ctatgtatcc cagtaccagc
      361 ttccctggct tacagagatc aatatgcggc ttgttccacg gattctgtac accgaaaacg
      421 atgacgtttg aggaacttga gcataccgcc gatgtgcgga tgaaaattac cgcaccgaca
      481 cttccggaac tgtttgccga aagcgggttt gcacttgctg aaaccttgta cggcgagtac
      541 cagcgggaac cggctgcggt gtcttttccc atcgaatccg agggaaaaga tagagaggaa
      601 ggtatcgtaa atttcctgtc agaactgttg ttcctcaccg aaacagagta tctcgtcccg
      661 gtctcctttg atctttgcgt ggaaggaact gcggttaggg gaactgttgc aggagtattg
      721 ttcgatcgag caaaacatgc gggcggcatc ggtgtgaaag gtatttcata ctcagggctg
      781 tccttcagag aaaccgccgc aggttatgaa ctcatcatca tctttgatat ctaaaccatg
      841 aacgatcaga tacgaaatat ttcagatacc gagtgggaga tcccgcggga ttttgtgccc
      901 ggtatgcggg ttccgggaac ctttttcctc tcccatcatc ttgccgaagg acttgaggaa
      961 ggggcactga cccagcttgc aaatgttgca acactgccgg gaattctccg ctgttcgctt
     1021 ggtatgcccg atatccactg gggatacgga tttcccatcg gcggagttgc cgcatttgac
     1081 gaggacgagg gaatcatttc tccgggcggt gtcgggtttg acatcaactg cggtgtccgg
     1141 atgatcacca caccgctgac tgcatcagac attcccgata tgaaaggact cattgaggat
     1201 ctgtttgcag cagtacccac cggagtcgga tcgaaaagtc cggtacggct cagtcagaat
     1261 gatctctccg atatacttac agatggtgcg gcaaatgcgg tggagatggg atatggagtg
     1321 gaggaggatg tccgccggtg cgaagagaac ggtgcgatgg ccggggcaaa gcttgagttt
     1381 gtcagtaaaa aagcccgggc acggggaatt cctcagtgtg gaacgcttgg ttccggcaat
     1441 cacttccttg aggttcaggc gattgatgag atcgcagatc caaagacggc ggagattttt
     1501 ggcgtgaagg aaggacagat ctgtgtgatg atccactgcg ggtcgcgggg actcggccac
     1561 caggtctgta cggatcatct gaaagtgctg gaagaggcgg tgaaaaagta caacatctcc
     1621 ctgccggacc ggcagcttgc ctgtgcccct ctgcattctc cggagggtga agcatacttt
     1681 ggcgcaatgg cggcctctgc aaactatgcc tgggcaaacc gtcagatcat tacacatctt
     1741 gtccgagaac ttctggaaaa gaagttcggt atcgcctatg aggagatgcc gctcgtgtat
     1801 gatgttgccc ataatgtggc aaagtgggag gaacacgaga tccccggtga cggaaaaggc
     1861 cgccgccgtg tctgtgttca ccgcaaggga gcaacacgcg cctttggtcc ggggagaaac
     1921 gagattgtgc aggagttccg ggatgcaggt cagccggtga ttattccagg aagcatggga
     1981 acatcttcgt atctgcttgc gggaacaaca accgcaatgg aacgaacgtt tggcagtacc
     2041 tgtcacggtg caggaagagt acagagcaga tcatctgcaa agcggagttt aacaggtacg
     2101 gcagtagccg aggatcttgc aagacgcggg attattgtcc gtgctccaaa cgccgcggca
     2161 attgcagagg aggcaccgga cgtgtacaag ccgagcagcg aggttgtaca ggtggtacat
     2221 gatgcaggaa tttcacggat cgttgcacgg ttccggcctc tcggggtgat caaaggatga
     2281 tggatacaca caatcacatt gcggtgttct gggacaacaa gatgatgttt caccgcctgg
     2341 ttgaggacgc gtgcggctac tgcgaggcgg taactcccct gatgctcgcg gcaccgtttt
     2401 acaaaggccg ctacacaggc atcattatcc caaccggatt tgggaatctg cagtactcaa
     2461 aactgcttcc ggcactcagg gcagtcagcg gacgcatcga agattatctt gagaacggcg
     2521 gaaaactgct tgtgtacggt gcggcagatc cggcaaatac cggtaacccg tacaactggc
     2581 ttccggtgga cgttgagtac cacttcgagt ttgccgagca taaactggag attgatgcgt
     2641 catccccatg gaaatcactc ttcgacgggt atgatacgga taaatttgca gcagacgggt
     2701 ggtttgtgaa gtatccggga aaaccggttg cagttgcaga gaacgggttt ccggtgttgg
     2761 tagagtgtcc cgtcggcgaa ggaacgcttc tcctcgcaag tgcacacgag tatccgtccg
     2821 atgcatttct caaacagttt ggtgccgcag ctaccgaaat ccagttctaa ctttttttcc
     2881 atcttgagcg aaatcttctt gtgggtctat gccgaaaatt gaaggtatag atgttccgga
     2941 gaaatccggg acggagtggt catgacacag acaatatttg atgttctgcg aatccttgac
     3001 gaggcaggta tcggcaatcc ggtacccgga gaggttatca gcgaacgcct tgggattacc
     3061 cgtaccgccg tatggaaata tgtgaatctg cttcgggagg taggatactg tattgacgca
     3121 tcaccgaaga cggggtattc actccagaag cgaacctggc ttttgctgcc gtatgaagtc
     3181 aaacggttcc tcaagacgca gaccattggt caggagatgc accactttga acaggttccc
     3241 tcaacaaatg cccttgcccg tcagatgctg catgaggtag gcaatgaggt ccagccggga
     3301 actgtactga ttgccgagca gcagacaggc ggccttggca gaatgaaccg tgtatggatg
     3361 agccctgaag gaggaatctg ggcgacaatt gtagtcatgc cgaagctgaa tgtcgatcag
     3421 acgttcagca tcatgatggc cggatccctt gccctcgccc gggcaatccg tcatgagttt
     3481 ggcctgtcag ctctgatcaa atggccgaac gatgtgttca tcggcgacaa aaaggttgca
     3541 ggaatgacga ttgagcttgc agcagatgaa gacattctcc gctactgtct gattggcgtc
     3601 ggaatcgatg taaacgtctc ggtggaaagg acaatgccga acatctcaaa gcttgtcacc
     3661 tccatctcgg atgagctcgg ccatgaagtg gagcgggccc agttctttgc aaagttcctg
     3721 aaagagtttg aacgccggta tcagatggtg ctgatgggtg aaaccgagcc gctgttccgc
     3781 gagtggcgca gtctgtccta cacactgcac cgccgggtcc gcatccggac aatgaaagag
     3841 agcttcgagg gagaggcaat tgacattgat gaacacgggg cactgctggt gcaccgcgac
     3901 ggcggagagg tcgaacgcgt gattgcgggc gactgcttca tgctctaatc attttctttt
     3961 tcagagtttg cgcatcttgt gaaaactccc tgataccata tacactgatt ttttatcccg
     4021 cttccaatca gcacccttat tgtttaccaa aatcaaacaa gtagtttaca atcatgtacg
     4081 gtaatgaaaa acgggcacgt ttgctcgcag agtcagcggc attcatcgcc ctcatcgcag
     4141 ttggcggatg gatatccatt ccaattctga taattccctt taccctgcaa aatatattca
     4201 tactgcttgc cgcagccgta atgaagcgat atgcagtaat tccggtcgga ctctatgtcc
     4261 tctttggatc acttggtctg ccgatcttcc ataacggaac cgcaggactt ggagtacttc
     4321 tcggaccgac cggaggattt ctgataggat ttgtgataat gggtgcagtt gcaggatggc
     4381 tgtttgaaaa agaaaccatc cgctatgaca tactggcctg cgctgcattt gtcactggcc
     4441 tttatgtctt cggaaccgcc tggtttatga tctcgaccgg agcagaactt acggcagccc
     4501 tcctcgcgtg tgctgtaccg tacattccag gagacatcat caaatgcgta attgtcgaag
     4561 cagtaattct ccggctgcgc agcgcaaaaa acaaaacatc acagacgaac aatgatatcc
     4621 atagctgatc tcaggtacgg cattgtggat attccccacc tttccatccc agagggaatc
     4681 tccgctgtta ccggagacaa tggtgcagga aaaaccacac ttctccgtct tgctgcagga
     4741 atgctgctgc cagaaacagg aacaatcacc atcaacggga ctgcaccgcg agcatgtgaa
     4801 gtcggttgtg tctccgagtt tccggaccgg catctgatct tttcggtagt acgtgacgag
     4861 atcgcatctc ccctccggtt cgcacaccgc aaacccgcag aaatagacga tgcagttacc
     4921 tcgatcacgg accggcttgg aattgcacat ctgctggaca gagagtgccg gac
//
LOCUS       DALUAJ010000098         4155 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_17524,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000098 DALUAJ010000000
VERSION     DALUAJ010000098.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17524"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            251..1744
                     /gene="fdhD"
                     /locus_tag="O0X37_02100"
     CDS             251..1744
                     /gene="fdhD"
                     /locus_tag="O0X37_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HJK61754.1"
                     /translation="MEEDMIASCRGYRYQNGNVTEIDDVVVREDTITLFLNGREYLRM
                     AATCDMLTELGAGFFTAAGIAERILSVRVKGTKVFVEAELRCDLTPDGFAPAKPARCI
                     TTEGVLIAPNEIFTLREALNTKVWKDTGGMHCAGLWYDHRCVVVANDIGRHNAVDKVI
                     GWMVLHNLPAKECMLGSTGRQPAGMVTKAVNAGIPVIAARAAATVKGAKRAEEMGVTL
                     IGFVREGRFTVYTHPERITELNPEAGEPQPAKNCGVLAAPLRGIRYREGKSIPVEDAA
                     VAEDLVTLFLNGKEFIRTVASGEHLRELAVGFFVDAGLVCSASDILSVRTEGANVFVE
                     ASCTACVAGELESAGGFTPLHARGRAHPGGEITPEEIFVIRDAINAEVWDATGGLHCA
                     VLFHDHRVVFLASDIGRHNAVDKVIGYMILAGLNPQECVIGSTGRQPAGMVMKAANAG
                     VPIVVSRAAATDAGIATAKECGVTLICFTRPPRFTVYAHPQRITGIL"
     gene            complement(1849..2985)
                     /locus_tag="O0X37_02105"
     CDS             complement(1849..2985)
                     /locus_tag="O0X37_02105"
                     /EC_number="2.1.1.213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004809 - tRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine(10)-N(2))-dimethyltransferase"
                     /protein_id="HJK61755.1"
                     /translation="MEYRQVTEGATTFEAPVQDEQTAFPPGTAPVFYNTKMEFNRDMT
                     VLLLSCISPENYLDAMAATGVRGLRVSHETGIPAIINDRDPAAVAMIEENAERIGGDI
                     RVICDDANRLMCTERFDAIDLDPFGTPAPFLDAASRSAKRYLFVTATDTAPLCGAQLK
                     AGLRRYFATPKNTEYHAEVGLRMMMGAMAKELVKYDRGMEPILSFAKNHYFRSHVRVL
                     GRVTAAEATMDQIGFVMQCPKCLYRAEQKGSLLPRSHTCPYCGAETEAIGPLWMGPLQ
                     DKEIISSLLEKLPSMRFGTAKQMDKLLSQLLREPDTCSFYDYHVVSRNLRVSPPAMDE
                     MLDGLNDLGYYTVRTHFSVTGIKTTAPLHVIEAWILAWNKERNL"
     gene            complement(3004..3078)
                     /locus_tag="O0X37_02110"
     tRNA            complement(3004..3078)
                     /locus_tag="O0X37_02110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3041..3043),aa:Arg,seq:cct)
     gene            complement(3266..3526)
                     /locus_tag="O0X37_02115"
     CDS             complement(3266..3526)
                     /locus_tag="O0X37_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 1-beta"
                     /protein_id="HJK61756.1"
                     /translation="MGEVAVILKVMPESPEVDMEKLQADIRAKVSGIQDMRVEPIGFG
                     LSAIKIAMITEDEEGSGDKIEGLFAGIAGIDRAEIESLNRML"
     gene            complement(3536..3697)
                     /locus_tag="O0X37_02120"
     CDS             complement(3536..3697)
                     /locus_tag="O0X37_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HJK61757.1"
                     /translation="MAATKCTSCNAPLAECGATEFKCPDCGEVIYRCAYCRKQSIKYT
                     CPKCGFQGP"
     gene            complement(3765..>4155)
                     /locus_tag="O0X37_02125"
     CDS             complement(3765..>4155)
                     /locus_tag="O0X37_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="uridylate kinase"
                     /protein_id="HJK61758.1"
                     /translation="YGWFLSTFGVAVDDVCSMPLAGAKILLPYRVLRDADPLPHSWDV
                     TSDTISAWIAGKLGCPLILLKSVDGVFLGGDLLEEISADIETDVIDPCCRGVLRRYAV
                     PAHIINGRCPDRLSAYLQGDCVPGTRL"
BASE COUNT         1107 a          949 c         1093 g         1006 t
ORIGIN      
        1 ggtgatacct gcgatctttg tcagcggttc gatgaatgca agcgtctctt caggagtttt
       61 gcttcgctcg gtctgtccgc agtaagtttt gggagacggc tgaagctcta tcattatcat
      121 cattcttcgg gcgcgaaggc tattgagggt ttcgttgtcg tacgccagtt cagtttttga
      181 gttcgttcgc tgaatttata tcctgttcgt ataaacatat gggcattcac gccgtgaaaa
      241 gagggaaaac atggaagagg atatgattgc ttcctgcaga ggataccgat atcagaacgg
      301 aaatgtcaca gagattgatg atgttgtggt acgcgaagat acaattacac tgtttctgaa
      361 cggaagagag tatctgcgga tggcagcaac ctgtgacatg ctcacagagc ttggcgcagg
      421 atttttcacg gcagcaggaa ttgcggaacg tattttatcg gtccgtgtaa agggaacaaa
      481 ggtgtttgtg gaggcggagt tgcggtgtga tctaacaccg gacgggtttg caccagcaaa
      541 accagcccgt tgtattacaa cagaaggggt actgattgca cccaacgaaa tttttacact
      601 tcgggaagca ctgaacacaa aagtctggaa agataccggg ggtatgcatt gtgcaggact
      661 gtggtatgat caccgttgtg ttgttgtggc aaatgatatt ggacgacaca atgcagtgga
      721 caaagtaatt ggctggatgg tactgcataa tctgcctgcg aaagagtgta tgctcggcag
      781 tactggaaga caaccggcag gcatggtgac aaaggcagtg aatgcaggaa tcccggtgat
      841 tgcagcacgc gctgccgcaa cagtgaaggg agcgaaacgt gcggaagaga tgggagtgac
      901 actgatcgga ttcgtacgcg aaggcaggtt tacagtatac acacatcctg agcgaatcac
      961 agaactaaat ccagaggcgg gagagccaca acctgcaaag aactgtggag tacttgcagc
     1021 accactccgt ggtattcgat atcgagaggg gaaatcaatt ccggtagagg atgcagcagt
     1081 tgcggaggat ttagtgacgc tgtttctgaa tggaaaagag tttatcagaa cagtggcaag
     1141 tggtgaacat ctgcgggaat tggcagtggg tttttttgtg gacgcaggcc tggtgtgctc
     1201 tgcctcagat attttatcgg tgcgcacaga gggagcaaac gtatttgtgg aagcatcatg
     1261 tacagcatgt gttgccgggg agctggaatc agcaggaggg tttacaccgc tgcatgcgcg
     1321 cggcagagca catcctggag gagaaattac accagaggag atctttgtaa tccgggatgc
     1381 aatcaatgca gaggtgtggg atgcaaccgg cggtcttcac tgtgcagttc tgttccatga
     1441 tcaccgtgtg gtattccttg catccgatat cggacggcat aatgcagttg acaaggtaat
     1501 cggctacatg attcttgcgg gacttaatcc ccaggaatgt gtaattggat ctactggaag
     1561 acagccggca gggatggtta tgaaggcagc aaatgcagga gttccaatcg tggtatcacg
     1621 tgcagcagca acagatgcag gaatagcaac ggcgaaagaa tgcggggtaa cactgatttg
     1681 tttcacccgg ccaccgcgat ttacagttta tgcgcatcca cagagaatca cagggatact
     1741 ctgatgtaga gagaatcgtt cgcttcaggg atttgcagat ccttcttttt tggaactggg
     1801 aatgtttgaa tacatcagct ccgggttgta cttcctctgc aacagattct acagattccg
     1861 ttctttattc catgcaagta tccatgcctc gatgacatgc agtggggccg tagttttgat
     1921 accggtgact gagaaatgag ttctgacagt gtagtatcca agatcgttca ggccatccag
     1981 catctcgtcc attgcgggag gggatacacg aaggttgcgg gagacaacat ggtagtcgta
     2041 aaagctacag gtatccggct cacggagaag ctgactgagc agtttgtcca tctgctttgc
     2101 ggtaccaaac cgcattgaag gaagtttttc aagaagcgag gagataattt ccttgtcctg
     2161 aagcggaccc atccaaagcg gacctatggc ttctgtttcc gcgccgcagt aaggacaggt
     2221 gtgagagcgg gggaggagag aacctttctg ttctgcacgg tagaggcatt tgggacactg
     2281 cattacaaag ccaatctgat ccatagtagc ttccgcagcg gtgacacgac caagtacacg
     2341 cacatgagat cggaagtagt ggtttttggc aaaactaaga atcggttcca tgccgcgatc
     2401 atatttaacg agctcttttg ccattgcacc catcatcatg cgaagtccga cttctgcatg
     2461 gtattcggta ttttttggtg tggcaaagta acgccgcaga cctgctttga gctgtgcccc
     2521 gcaaagaggg gcagtgtccg ttgcagtgac aaagaggtag cgtttcgcgg aacgtgatgc
     2581 agcatcaagg aagggagcag gagtaccgaa cggatcgaga tcaatcgcat cgaagcgttc
     2641 agtacacatg agtcggttgg catcatcaca gataacgcgg atatcaccgc caattctttc
     2701 tgcgttctcc tcaatcattg cgacagcagc agggtcgcga tcgttgatga ttgcaggaat
     2761 cccagtttca tgggatacac gaagaccgcg gacaccggtt gctgccatcg catcgaggta
     2821 attttctgga gagatacagg agaggagaag aacggtcatg tcacggttga actccatctt
     2881 tgtgttgtag aagacaggag cagttcccgg ggggaatgcg gtttgctcgt cctgtactgg
     2941 ggcttcaaag gtggttgccc cttccgtgac ctgtcgatat tccataatgc atctccaaaa
     3001 agacggacat gtagggattc gaaccctaga tcttcagctt aggaggctga tgccatatcc
     3061 tggcttggct acatgtccag aaggtgagga tatatgtttg gagagacgcg ggtatatctt
     3121 ttacgtaagc atgatcctaa aatgaaccaa accttctgaa acatacagac aatacacatg
     3181 gtatcctcca tttgttttga tgctgcaaaa ccccaaacac gtaccgcagc gaaacactat
     3241 cgaagtgtga aaagaaaata agtatttaga gcatccggtt cagagattca atctctgcac
     3301 gatcgatgcc tgcaattcct gcaaagagcc cctcaatctt gtcgccggaa ccttcctcat
     3361 cctcggtgat catggcgatc ttgattgcag agaggccaaa tccaatcggc tcaactctca
     3421 tatcctgaat tccggaaacc tttgctctga tatcagcctg aagtttttcc atgtcaactt
     3481 ccggagactc cggcatgacc tttaaaatta ctgcaacttc gcccatatta tttcctcatg
     3541 gtccctggaa tccgcacttc ggacacgtgt atttaatgct ctgcttacgg cagtatgcac
     3601 aacggtagat aacttcaccg cagtcaggac acttgaactc ggttgcaccg cactcggcga
     3661 gtggggcatt acatgacgta catttcgtag ctgccattcg atcaaactct ctagactatt
     3721 agctccctac aattataaac gtgtggcctc tggcacgcac aatatcacaa cctcgtaccg
     3781 ggaacgcaat ctccctgcaa ataggcagac aggcgatccg gacatctccc attgataata
     3841 tgtgctggaa ccgcatatct gcgcagaaca cccctgcagc agggatcaat tacatcagtc
     3901 tcaatatccg ctgagatttc ctcaagcaga tctcctccca aaaaaactcc atcaactgat
     3961 ttcaaaagaa tcagagggca gcccagcttg cctgcaatcc atgcagatat tgtatccgac
     4021 gtcacatccc atgaatgcgg aagaggatct gcatcccgca aaacccgata cggaagaaga
     4081 atttttgcac cagccagagg cattgagcaa acatcatcca ctgcgacacc aaacgttgac
     4141 aaaaaccacc cgtac
//
LOCUS       DALUAJ010000099         4227 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_17896,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000099 DALUAJ010000000
VERSION     DALUAJ010000099.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4227)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4227)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4227
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17896"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="O0X37_02130"
     CDS             <1..914
                     /locus_tag="O0X37_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit A''"
                     /protein_id="HJK61759.1"
                     /translation="LGLPRLIEIMDARKEPSTPTMTIFLEKGYREDRDKAREVSWKIE
                     AAPLHEFGDIETDIADMCVIVQINREVCKKRKISVAEILERAPQKIKDKRHYRDFEVE
                     VDEKEAILRFLPKNEESYQNLFQLAEHVRQVIVQGIDDIRRVVVRKENDEYILHTEGS
                     NLKDVFEIDGVDCTRTRTNNIAEIANTLGIEAARSAIIYEAFSTLKEQGISVDLRHIM
                     LVADIMCMDGEVKQIGRHGIAGEKESVLSRASFEVTVNHLLDAAVAHEFDELSGVTEN
                     VIVGQPILLGTGDVKLMVRRVNPTKSA"
     gene            1014..1445
                     /locus_tag="O0X37_02135"
     CDS             1014..1445
                     /locus_tag="O0X37_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HJK61760.1"
                     /translation="MSVTNGNTIRVHYTGTLTDGTQFDSSEGRDPLEFVVGSGMVIPG
                     FDKGVVGMEIGETRTLEIPAKDAYGPRSDDMTVAIPRSEFGSDFTAEIGEQLLIQLSD
                     GNQIPVTITQIDDKTVTLDANHKLAGKDLIFTVTLVEIVAE"
     gene            complement(1537..2112)
                     /locus_tag="O0X37_02140"
     CDS             complement(1537..2112)
                     /locus_tag="O0X37_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HJK61761.1"
                     /translation="MKRELPAYAVLPPCPVTVVCTYDADEKPNGLAVSWTGIAVSEPP
                     HVMIAVRPGRYSHAALCERGEFTMNIPSEDQMCQADYFGITSGRKTNKFEDLDLTPMP
                     AEHVHAPMIAEFPLSLECRVVSRQEIGSHSVFIAEILAVHADERILDAEGKIDVVLLR
                     PLAYDHAKYLYLAQGQVLGRAFSAGKILKKS"
     gene            2148..2405
                     /locus_tag="O0X37_02145"
     CDS             2148..2405
                     /locus_tag="O0X37_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HJK61762.1"
                     /translation="MEKPLYRSANDRWLAGVCGGIAEYAKIPSILVRLIFIALCLVLL
                     PFWTIILILFYIVASFYLPEAPSRKTIEDPNVIDAEFEVKE"
     gene            2412..3014
                     /locus_tag="O0X37_02150"
     CDS             2412..3014
                     /locus_tag="O0X37_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5591 domain-containing protein"
                     /protein_id="HJK61763.1"
                     /translation="MPSGSRSDLTEPPFYLPQFEQAYRYIIDEYLVQPRETAIFIPCA
                     VRKPYSQSPSHKLFHGMFHDVFPDQGRYHVVIFGTCGTVPAELELMYPYAHYHYMLGN
                     VKDQRIKDDFLRIETRRLIGYLEKTRFTYEKRIAYCIGIFRAAMEAATAETKIPVEIY
                     PTRPVIEKLYDADCPFPEGSLSMQEYLDEFRAALIRVRDA"
     gene            3017..3394
                     /locus_tag="O0X37_02155"
     CDS             3017..3394
                     /locus_tag="O0X37_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HJK61764.1"
                     /translation="MAVREVSVVRLDEKKSKFYAHLYEIDTVDDVLEVRAIHEKLYKK
                     AAHHCYAMVCGNVTDSRADGEVGSPGRALAEVMERNNLGSHVLMVSRIFGGIKLGPAG
                     VARAFREAGAGAVAEYQKTRKKL"
     gene            3418..3606
                     /locus_tag="O0X37_02160"
     CDS             3418..3606
                     /locus_tag="O0X37_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61765.1"
                     /translation="MDQLRGCPMANGSTCTAENCQWWNDEWKDCAVNVMVRYLRAQDV
                     RATFDHAVPAEFLEGSRK"
     gene            3607..>4227
                     /gene="surE"
                     /locus_tag="O0X37_02165"
     CDS             3607..>4227
                     /gene="surE"
                     /locus_tag="O0X37_02165"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HJK61766.1"
                     /translation="MRPKILLTNDDGVNSGGLWAAYEALSAFADVTVVAPAVQQSAVG
                     RSISIFEPLRMNEVWIGDRSAYSVEGRPTDALLLGLYGLNLKPDLVVSGINLGENISY
                     ESITTSGTVGAAMEAANQGVPALAYSLQMSDQGNKFTDPRCHTQDFSQAKEVVAKLTR
                     IFLASGLPKNCHLVNINIPACRMEGYEVTTLGTRLFDTYVEKRIDPR"
BASE COUNT         1020 a         1026 c         1118 g         1063 t
ORIGIN      
        1 ccttaggtct gccgcgtctc attgagatta tggacgcacg caaggaaccg tccacaccga
       61 cgatgaccat cttccttgag aaggggtacc gtgaggatcg tgacaaggcc cgtgaggttt
      121 catggaagat cgaggctgct ccgctgcacg agttcggtga tatcgagacg gacattgcgg
      181 atatgtgtgt gattgtgcag atcaatcgcg aggtgtgcaa gaagagaaag atctccgttg
      241 ccgagatctt agagcgcgct ccgcagaaga ttaaagacaa gcgccactac cgtgatttcg
      301 aggtggaggt tgacgaaaag gaggctatcc tccgtttcct cccgaaaaac gaggagtcat
      361 accagaacct gttccagctt gccgagcatg tccgccaggt aattgtgcag ggtattgatg
      421 atatccgccg tgtggtggtc agaaaggaga atgatgagta cattctccat acggaaggtt
      481 ctaacctgaa agacgtcttt gagattgatg gtgtggactg taccagaacg cgtacaaaca
      541 acattgcaga gattgcaaac acgcttggta ttgaggcagc acgttccgca attatctatg
      601 aggcattctc aaccctgaag gaacagggta tctctgtgga tctccgccac atcatgcttg
      661 ttgcagatat tatgtgcatg gatggtgagg tcaaacagat cggtcgtcac ggtattgcag
      721 gagaaaaaga gtcggttctc tcccgtgcat ccttcgaggt tacagttaac cacctgctgg
      781 acgccgcagt tgcccatgag tttgatgagc tgtccggtgt gacggagaac gttatcgttg
      841 gtcagccgat tcttctcggt acaggcgatg tgaagctgat ggttcgccgg gttaacccga
      901 cgaaatcagc ataatacttc ttttcttttt tgatgttttc ctgtcgtgta gcgttcacgc
      961 gaaacggttt cttttccgaa attacttaac ttcgcacaac ccacatgagc ggtatgtctg
     1021 ttacaaacgg caatacgata cgcgttcatt ataccggaac tctcaccgac ggtacacagt
     1081 ttgactcttc ggagggccgt gacccgctgg agtttgttgt cggtagcggc atggtgatcc
     1141 ccggattcga caaaggtgtt gtcggtatgg aaatagggga gacccgtact ctggagatcc
     1201 ctgcaaagga tgcctatggg ccaagatctg atgatatgac cgtcgccatc ccgcgcagcg
     1261 agtttggttc tgactttacc gcagagatcg gtgagcagct gttaatccag ctgagtgacg
     1321 gaaatcagat tcctgtgacc attacccaga ttgatgacaa aactgtaacg cttgatgcaa
     1381 accacaagct cgcaggaaag gatctgatct ttaccgtgac gcttgtggag atcgttgcag
     1441 aataagatat ccttcaaaaa tatttttccg ggtgatgaag acaattcatt tcatcctgtc
     1501 aacctcatcc gtttttttcg tgagttacag atcctgtcat gattttttca gtatctttcc
     1561 cgcggaaaat gcccgtccca gtacctgccc ctgcgcgaga taaagatatt ttgcgtgatc
     1621 atatgccaga ggccgcagta acaccacatc gatcttccct tccgcgtcca gaatgcgctc
     1681 gtcggcatgt actgcgagaa tctctgcaat aaacaccgag tgtgatccga tctcctgtcg
     1741 tgagacgact cggcattcaa gagagagcgg gaactctgca atcatggggg cgtgcacgtg
     1801 ctcagcaggc atcggcgtca gatcgagatc ctcgaattta ttcgtctttc tgccgctggt
     1861 gattccgaaa taatccgctt ggcacatctg atcctctgag gggatattca ttgtgaactc
     1921 tccccgctca cagagtgctg catgactgta gcggccggga cgcacggcaa tcatcacatg
     1981 cggcggttcg gacacggcaa tccctgtcca tgacactgca aggccgtttg gtttttcatc
     2041 ggcatcatac gtacagacaa cggttaccgg gcatggaggc agaaccgcat aggcaggcaa
     2101 ctcacgcttc atacaactat atttttgcag caggacaata tgttagtatg gaaaaacctc
     2161 tctaccgttc tgcaaacgac cgctggcttg caggagtctg cggaggcatt gctgaatacg
     2221 caaaaattcc gtcaatactt gtcaggctga tctttattgc cttgtgcctt gttctgctcc
     2281 cgttctggac catcattctg atcctgtttt acattgtggc atccttctat ctccctgaag
     2341 caccttcgcg aaaaaccatt gaagatccca atgttatcga tgcagagttc gaggtaaagg
     2401 agtaatcccg catgccgtcc ggctcccgca gtgatcttac cgaaccaccg ttttatcttc
     2461 cgcagttcga gcaggcctac cgctacatca tcgatgaata tttggtgcag ccgcgcgaaa
     2521 ccgcgatttt tattccgtgt gctgttcgaa aaccttacag tcaaagcccg agccacaaac
     2581 tgtttcatgg catgtttcac gatgtttttc ccgatcaagg gaggtatcat gttgtcatat
     2641 ttggtacctg cggtacggtt cccgcagagc ttgagctgat gtatccctat gcccattatc
     2701 actacatgct tggcaacgtg aaggaccagc gtatcaaaga tgattttctc cggattgaga
     2761 ccaggcgtct tatcgggtat ctcgaaaaaa cgaggtttac ctatgaaaaa cgcattgcct
     2821 actgtatcgg catcttccgc gccgcaatgg aggctgcaac tgctgaaaca aaaattccgg
     2881 ttgagattta tccaacccgt ccggtgatcg aaaaactcta tgacgctgac tgtccgtttc
     2941 cggaaggaag cctttccatg caggagtacc ttgatgagtt ccgcgcagca ctgattcgtg
     3001 tgagggatgc ataagaatgg ccgtccgcga agtttccgtt gtccggctgg atgaaaaaaa
     3061 gtccaagttc tatgcgcatc tctatgagat tgacacagtc gacgatgttt tggaggtgcg
     3121 tgcaatccat gaaaaactct acaaaaaagc tgcccatcac tgttatgcaa tggtctgtgg
     3181 aaatgttacc gacagtcgtg ccgacggaga ggttggttct cccggccgcg cacttgccga
     3241 agtgatggag agaaacaatc tcggatcgca tgttcttatg gtttcccgca tcttcggcgg
     3301 catcaaactc ggaccagcgg gtgttgcccg tgcattccgt gaggctggtg caggtgcggt
     3361 ggcggagtac cagaagacgc ggaagaagtt atagctccaa aaagaaaaca tagtagcatg
     3421 gatcaattac gcggatgccc gatggcgaac ggcagtacct gtactgcgga gaactgccag
     3481 tggtggaacg atgagtggaa ggactgtgca gtgaatgtga tggtccgtta tctgcgggca
     3541 caggatgttc gtgcaacctt tgatcatgcg gttcctgctg agttccttga aggcagcagg
     3601 aaataaatgc ggccaaaaat tcttctcact aacgatgacg gtgtgaactc cggaggtctg
     3661 tgggcagcgt atgaggcact gtcagcgttt gcggatgtga cagtggtagc gcctgctgtg
     3721 cagcagagtg cggtcggcag atcaatctcg atctttgaac cgctcaggat gaatgaggta
     3781 tggatcggtg accggtcggc gtacagtgtg gaaggcagac ctactgatgc gctgctgctt
     3841 ggcctttacg gtctgaatct caaacctgat ttggtggtga gcgggatcaa tctcggtgaa
     3901 aatatttcgt acgaatcaat tactacctcc ggaacggtcg gcgctgcgat ggaagccgca
     3961 aaccaggggg ttcctgcact tgcctactcg ctgcagatga gtgatcaggg aaacaagttt
     4021 accgatccgc gctgccacac gcaggacttt tcccaggcca aagaggttgt agcaaagctg
     4081 acgcggatct ttcttgcatc cggtcttccg aaaaactgcc atctggtaaa tatcaatatc
     4141 cctgcctgcc gtatggaggg ctatgaggtt accacacttg gaacgcgtct gtttgatacg
     4201 tatgtggaaa aacgaatcga tccccgc
//
LOCUS       DALUAJ010000100         2189 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_18107,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000100 DALUAJ010000000
VERSION     DALUAJ010000100.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18107"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(138..902)
                     /gene="mtxX"
                     /locus_tag="O0X37_02170"
     CDS             complement(138..902)
                     /gene="mtxX"
                     /locus_tag="O0X37_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker protein Mmp4/MtxX"
                     /protein_id="HJK61767.1"
                     /translation="MTIGIGCGADAWKVVQSANETATEDLHIRCYCCPGAVDDLSSGP
                     FVELAVCSLPYQRIVDDLLAGKINGAVRGTLPANETLRYLKKACGVDRLERIALLETV
                     DHQCFFLAPVGVDEGWTIQEKISLINDGRELARRFGLSEKTAVLAGGRIGDIGRHPIV
                     DKTISDAQEIAQLTGAVHGEILIEDAVRDCGVIIAPDGISGNLIFRTLTFLGHGVGHG
                     APVVNISPVFVDSSRASADYGGSLRLAAALSTGKKH"
     gene            complement(902..1888)
                     /locus_tag="O0X37_02175"
     CDS             complement(902..1888)
                     /locus_tag="O0X37_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 2 protein"
                     /protein_id="HJK61768.1"
                     /translation="MVADNYTPESVANAVRQYDGVRRKQAIGTLVRALHIDNLDVIAS
                     YGEDAAVIQNGRTALLLAADGIWNKLMEVDPYWAGYCAILVNVHDIAAMGGRPVAMVD
                     VLSASNDELMEEVSRGMRDAATAFDVPVVGGHLHPDAPYNVIDVSILGVAELENIIYS
                     SKAEPGNAIIVAIDLNGRIHPSGPMKWDSVTMKTPEVLRAQIGVMKDLGEHHHLTAGK
                     DISNPGVIGTLGMLIESSGVGAVVDLTAVPHPDLDALGITFEQWVRMYPGMGFIMTAD
                     QSQVDIVCKKFRAVGMAAQIIGTVTNTKRLTLVKGDSETVLFNFCAEGITNI"
BASE COUNT          636 a          519 c          513 g          521 t
ORIGIN      
        1 atataaatat atctcatgta tggggtgttt ttcgcagttt gccggctttg gtgaaccgaa
       61 tatgactttt tgcaaagaat atggacgata tttgaaatta attatggtaa cggaccagat
      121 ttgaaaaata acaaaattta atgctttttt ccagttgaca aggctgctgc aaggcggaga
      181 gatcctccat agtccgcaga tgcgcgcgaa ctgtccacaa aaacgggaga tatgttaaca
      241 acaggtgcac catgtcctac tccatgacca aggaatgtga gggtccgaaa gatcaggttt
      301 ccagagatac catcaggagc aatgataaca ccgcagtccc ttactgcatc ctcaataaga
      361 atttctccat gaacagcacc ggtaagctgt gcgatctcct gggcatcaga gatggtttta
      421 tccacaatag gatgtctccc aatatcaccg attctgccgc cagcaagaac agcggtcttc
      481 tcagagagac caaaccggcg ggcaagctcc cgaccgtcat tgatcaaaga gattttttcc
      541 tgaatggtcc atccctcatc aacaccaaca ggtgcaagga aaaagcactg gtgatcaacc
      601 gtttcaagaa gagctatccg ctcgagacga tcgacaccac aggctttttt aagataccgc
      661 aatgtctcat tggcaggaag agtcccgcga acagcaccat tgatcttccc agcaaggaga
      721 tcatcaacga tgcgttgata aggtagagaa cagacagcaa gctcaacaaa gggaccggaa
      781 gacaaatcgt caacagcccc agggcagcag taacagcgga tatgcaggtc ttcggttgcg
      841 gtttcattcg cactttggac gactttccaa gcatccgcac cacaaccgat tccaatcgtc
      901 atcatatatt agtgataccc tctgcacaga aattgaagag tacagtctca gaatcccctt
      961 tgacaagagt gagtcgtttg gtgttggtaa cagtaccgat tatctgagca gccataccaa
     1021 ctgcacggaa ttttttgcag actatgtcaa cctgggactg atccgcagtc atgataaaac
     1081 ccattccagg gtacatacgc acccattgtt cgaaggtgat gccaagagca tcaagatcag
     1141 ggtgaggtac tgcggtaaga tcaacaacgg cgccaacacc actggactca atgagcattc
     1201 caagggttcc aatgacacct gggttggaga tatcttttcc ggcggtaaga tggtggtgtt
     1261 caccaagatc tttcatgaca cctatctggg cgcgaaggac ttccggagtt ttcatggtga
     1321 cagagtccca tttcatgggt cctgagggat ggatacggcc gttgagatca atggcaacga
     1381 tgatggcgtt acccggttct gctttgctgc tgtagatgat gttttcaagt tcggccacac
     1441 caagaatcga gacatcaatg acgttatacg gagcatcggg gtggaggtga cccccgacaa
     1501 caggaacatc aaaagcggtt gccgcatcgc gcataccgcg gctgacttcc tccataagct
     1561 catcgtttga tgcggagaga acatcaacca tcgcaacggg tctccccccc attgcagcaa
     1621 tatcgtgaac gttaacgaga atggcacagt aacctgccca gtacgggtca acttccatga
     1681 gtttgttcca gattccatct gctgcaagca gaagtgcagt acgtccgttc tgaataacgg
     1741 cggcatcctc accatatgat gcgatgacgt cgaggttgtc gatatgaagc gcacgaacga
     1801 gggttcctat tgcctgtttg cgtctgacac cgtcgtactg cctgactgcg tttgcaactg
     1861 attcaggggt ataattgtct gcaaccattg ttctctctgt caccaagggg catggcagcc
     1921 ccagttgtta cctattgtat tcccgttcaa cggtgataag atgcacgatt aggattgggc
     1981 gagatacatc ctggaaaatt tgcatttaga acagaaataa aaacgagttt cacacattat
     2041 catatattta tcatttaaaa aagggaatca caccacataa agacagggat ttgagaaata
     2101 tcagccacag atggtttaac cctctgcaga aaggccaaga aactgataca caaacgtgaa
     2161 taaatatatg taaatcagat gagaaaaag
//
LOCUS       DALUAJ010000101         5919 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_18116,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000101 DALUAJ010000000
VERSION     DALUAJ010000101.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5919)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5919)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5919
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18116"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="O0X37_02180"
     CDS             complement(<1..437)
                     /locus_tag="O0X37_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HJK61769.1"
                     /translation="MELDKYDQALLDGEYGESRQKMMEILLALGKIYDAERFIPVKSV
                     QVSGASFKTIGEAGLEWLSSIDAKAVVPAFLNPIGMPRTGWEEIGIPQSFAEKQIQVN
                     EAYIRLGIRPTCTCTPYYFSIVERGDHLAWSESSAVSYANSVLG"
     gene            complement(451..1692)
                     /locus_tag="O0X37_02185"
     CDS             complement(451..1692)
                     /locus_tag="O0X37_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HJK61770.1"
                     /translation="MRDFIKRMMAEGLVEEITDPVSSVYEAPKRAFTSKKMLYFHNCD
                     GHRCVMNAIYDRKSLSVALGIPEDKLVKTLAACTYAGKTHDAGKLSFGPAKLSDLPIM
                     KHFPKDAGRYLTSGIVFSAWGGVENASIHRMEVLDDTHLIGRIVEGRHTYKLLQQAKA
                     AGEKLPIAVVIGAHPAVTFAACTRVPEGKEMAYAAEILGEDMPLYECPNGIRVPDAEI
                     VLYGYMTAELHEEGPFVDISGTYDPIRMQPVIELCGMVCKPDYIYHGIVPAGAEHKML
                     MGAPYEPRIYQAAANVTNVRDVFLTPGGAGYFHAVVQVKKMTNGDGKNVIMAAFAAHT
                     SLKHVVVVDEDINIYDPNDVEFAIATRVRADQDVMIISGVRGSSLDPCRIGDGMNVKM
                     GIDATMQLGHEDEFIRADWNE"
     gene            complement(1689..2237)
                     /locus_tag="O0X37_02190"
     CDS             complement(1689..2237)
                     /locus_tag="O0X37_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HJK61771.1"
                     /translation="MKRIVVGVTGASGTLYAKRLIEALTKTDGVEVYLIVSDTARTVA
                     ELEGVDLSGYPVQYEENCDLSAGIASGSFLFDAMIVIPCSMKSLAAIANGYSTTLIAR
                     AADVALKERRKLILVPRETPYSRVHLTNMLAAHDAGAVIMPASPPLYTHPQTITDLAD
                     MIAGRVLDHCGIPHTIGSRWKE"
     gene            complement(2243..3448)
                     /locus_tag="O0X37_02195"
     CDS             complement(2243..3448)
                     /locus_tag="O0X37_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HJK61772.1"
                     /translation="MPKQIKDPVHGYIEVPAEILPLVDSPALQRLHHIRQLGFAYLVY
                     PGANHTRFEHSLGAMHLASLLCRHLGFGTAETRTICCAALLHDIGHGPYSHASERLMQ
                     EYTQFSHDDIREQLKEPILAKQLEKNSISPDEVAALISGSHRYAGIIHGDLDVDRMDY
                     LLRDAHYTGAPYGNFDAERLTKSLAVVDNTIVLNDSGISAAESLLIARTLMGPSVYYH
                     HACRIAEEMFLLACRSHFRKDIDAVREFLTMDDVTATALLLNSDEEITRDLINRIRTR
                     KLYKRAVYAGKELVDRDHLPQTKSGIERLHTAIAETAGVAPDKIILDIPPLRKEMKMD
                     IKVRNHHDLVPFEEIVPMIAMMNATRQGQWRLGVYAPAELRERVSDAAREVLALRRAT
                     KQHKLTGII"
     gene            complement(3455..3847)
                     /locus_tag="O0X37_02200"
     CDS             complement(3455..3847)
                     /locus_tag="O0X37_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61773.1"
                     /translation="MNKTEIIAVTFAVLVVFCGIGAGIGGLIVEEANGNYVQGLADKT
                     GTEIVKLTANDGSSAAAFRTADGVYIDLLSMINAYNGLTDVVVVYNVNNGQITGEQNA
                     AISFNHAAGLLSSPAGALLFNLAEAGQN"
     gene            3980..4378
                     /locus_tag="O0X37_02205"
     CDS             3980..4378
                     /locus_tag="O0X37_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HJK61774.1"
                     /translation="MITLDHGATSENAEHQDICFCPLHGLLDTISKKWALMIIAVIGN
                     HGSAGFNELKRQLCNVSSKTLSNTLKDLEEHGLVYRQVVDQTPPVVRYYLTVSGWELR
                     ELLIPLLAWVMKNGGHADEGCPIHFNTKNV"
     gene            4463..4544
                     /locus_tag="O0X37_02210"
     tRNA            4463..4544
                     /locus_tag="O0X37_02210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4497..4499,aa:Leu,seq:caa)
     gene            4601..5191
                     /locus_tag="O0X37_02215"
     CDS             4601..5191
                     /locus_tag="O0X37_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HJK61775.1"
                     /translation="MTPRILAISTSPRRHGNSESALDTVLAEIGDRFPAEKVVLGDLA
                     IAPCKGCGACERLGRCVQEDDFQSLSQKILDADVLILATPVYSMSVCAQAKALIDRCQ
                     VFWSRKYVLHTFEEPAGNKLGLFIATAGQTRDNIFDHTVPVARFLFDVSGIKPKHTML
                     LLLKGLDKKNDYLEDASAVAKTKEITASLLASLEVV"
     gene            5188..5772
                     /locus_tag="O0X37_02220"
     CDS             5188..5772
                     /locus_tag="O0X37_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HJK61776.1"
                     /translation="MTLITAVNGSPRKHGNTETVLDAFLRGAESTGAETNKLSLVDID
                     HKNCRGCNACHKKGVCILKDDLTPIFDAVLASDILVLASPIYSMTVTAEMKSFIDRGQ
                     FLWAQKFVTKTLSFSDEHLKHHIGVYLGTSGQEIPHIFDAAFPVVRAFFNDAGFSYTE
                     NVLFAGMDQHGGVKGWPESVAFAEAEGKRIAELI"
BASE COUNT         1334 a         1538 c         1470 g         1577 t
ORIGIN      
        1 ccaagtactg agtttgcata gctgaccgcc gaactctccg accatgcaag atgatcgccg
       61 cgttccacga tgctgaagta atacggtgta caggtacagg tcggccggat accaaggcgg
      121 atgtatgcct cgtttacctg aatctgcttc tctgcaaaac tctgcggaat accaatctcc
      181 tcccaaccgg ttctgggcat gcctatgggg ttgagaaatg ccggtaccac tgcttttgca
      241 tcaatgcttg acagccactc aaggcccgcc tcgccgatgg tcttgaacga tgctcccgaa
      301 acctgaacac tctttaccgg aataaaccgc tcagcatcgt aaatttttcc gagtgccagc
      361 agaatttcca tcatcttctg gcggctttca ccgtactcgc catccagcag cgcttgatcg
      421 tacttatcca gttccatatt cactccttac ttattcattc cagtctgcac ggataaactc
      481 atcctcatgc ccgagctgca ttgttgcatc gattcccatc ttcacattca tgccgtcgcc
      541 aatccggcag ggatcaagcg aggaaccgcg gacaccggag atgatcatca cgtcctgatc
      601 cgcccggacc cgtgttgcaa ttgcaaactc cacatcattt ggatcataga tgttgatgtc
      661 ctcgtccacg accactacat gtttgagcga ggtgtgtgct gcgaatgctg ccatgatgac
      721 gtttttaccg tcaccgtttg tcatcttctt gacctgaacc accgcatgga agtatcctgc
      781 tcctcccggg gtaaggaaca catcacggac gtttgttacg ttcgccgctg cctgatagat
      841 gcgcggctca tagggagcac ccatcagcat cttgtgttct gctcctgcgg gaacaatacc
      901 gtggtaaata tagtccggtt tacagaccat cccgcagagt tcaattaccg gctgcatccg
      961 gatgggatca tatgttccgc tgatgtccac gaatggtcct tcctcatgca gctctgctgt
     1021 catgtatccg tagaggacga tctctgcatc cggaacacgg atgccgttcg gacattcata
     1081 cagcggcata tcctctccaa ggatctctgc ggcgtatgcc atctcctttc cttccggaac
     1141 cctggtacat gcggcaaagg ttaccgccgg gtgtgcgccg atcacaactg cgatcggaag
     1201 tttttctccg gcagcttttg cctgctgcaa aagtttgtac gtgtgacggc cttccacaat
     1261 tcttccgatc agatgggtgt cgtcaagaac ctccatccgg tggatggatg cgttctctac
     1321 ccctccccat gcggagaaca caattcctga ggtcaggtat cggcctgcat ctttggggaa
     1381 gtgtttcatg atcggcaggt ccgagagctt tgccggaccg aaggaaagtt ttcctgcatc
     1441 atgagttttc cctgcgtagg tacatgcagc aagtgttttg acgagtttgt cctccgggat
     1501 tcccagtgca accgagaggc ttttgcgatc atagatcgcg ttcattacac atctgtgtcc
     1561 gtcgcagttg tggaagtaga gcatcttttt actggtaaat gcccgcttcg gtgcttcata
     1621 gacagaagaa acaggatcgg ttatctcttc aacgagtcct tctgccatca ttcgtttgat
     1681 aaaatctctc attctttcca ccgtgatcca atagtgtggg gtattccgca gtgatccagt
     1741 acgcgccctg cgatcatatc ggcaagatca gtgatcgttt gcgggtgtgt gtacagcgga
     1801 ggggaagcgg gcatgattac cgctcctgca tcatgggcgg caagcatatt ggtcagatgt
     1861 acccgggagt atggtgtctc tcttggaaca aggatcagtt ttcgccgctc tttcagggca
     1921 acatccgccg cccgtgcgat cagggttgtt gagtagccgt ttgcaattgc tgcaaggctc
     1981 ttcatactgc acgggataac aatcatggca tcaaacaaaa atgatccgct ggcaattcct
     2041 gcggagagat cacagttctc ctcgtactgt accgggtagc cggaaagatc cactccttcc
     2101 agctctgcga cggttcttgc ggtgtcggag acgatcagat acacctcgac gccgtccgtt
     2161 tttgtgaggg cttcaatcag ccgttttgcg tacagcgtcc cggaagctcc ggtgactccg
     2221 acaacgatcc gcttcatcct tcttatatta tacctgtgag tttatgttgt tttgttgcac
     2281 gccgcagtgc aagtacctcg cgggctgcat cactcacgcg ctcgcggagt tcggcaggcg
     2341 catacacccc gagccgccac tgtccctgac gcgtggcgtt catcatggca atcataggaa
     2401 caatctcttc aaacggtacg agatcatgat gattacggac cttgatgtcc atcttcatct
     2461 cttttctgag aggaggaatg tcaagaatga tcttatccgg cgcaaccccc gcggtttcgg
     2521 cgattgcggt gtgcagtcgt tcaattccgc tcttggtctg agggagatga tcccggtcaa
     2581 ccagttcctt cccagcgtac actgcccgtt tgtagagttt tcgtgtgcgg atgcggttga
     2641 tgagatcgcg ggttatctcc tcatccgagt ttagcagaag agctgttgcg gtcacatcgt
     2701 ccattgtcag aaactcgcgg accgcgtcta tatctttcct gaaatgactg cggcatgcaa
     2761 gaagaaacat ctcctctgca atccggcagg cgtgatggta atagacggac gggcccatta
     2821 gtgttcgtgc aatcagaagt gactcagcag ccgagattcc tgagtcgttg agaacgatcg
     2881 tgttgtcaac aactgcaaga gacttcgtca gccgttctgc atcgaagtta ccgtacggtg
     2941 caccggtgta gtgggcatcc cgcaggagat agtccatgcg gtccacatcc aggtcaccat
     3001 ggatgatgcc ggcatatctg tggctgccgg agatgagcgc tgcgacttca tcagggctta
     3061 tggaattttt ctcaagctgt tttgcaagga tgggttcttt cagctgctca cgaatatcat
     3121 catgtgaaaa ctgggtgtac tcctgcatca gtcgttcgct tgcatgtgaa tacggtccgt
     3181 gaccgatgtc gtggagaagt gcagcacaac agatcgtccg tgtctctgca gttccgaacc
     3241 cgagatgtct gcataaaaga gatgcgaggt gcatcgcacc aagagaatgt tcaaaccttg
     3301 tatggtttgc tccgggatac accagatatg caaagccaag ctgacggatg tggtgaagcc
     3361 gctgcagtgc cggtgagtcc accagcggca gaatctctgc ggggacttct atatatccgt
     3421 gtaccgggtc tttgatctgc tttggcatga ggatctagtt ttgtcctgcc tctgcaaggt
     3481 taaacagcag agctcctgcg ggtgagctga gaagtcctgc ggcatggttg aaggatattg
     3541 ctgcgttctg ttcaccggtg atctgtccgt tgttgacgtt gtacaccaca acgacatcgg
     3601 tcagtccgtt gtaggcattt atcatagaga gaagatcgat gtagacgccg tctgcagtac
     3661 ggaatgcagc ggcagatgat ccgtcattgg cagtcagttt tacgatctcg gttccggttt
     3721 tgtctgccag tccctgaaca tagtttccgt ttgcttcctc tacaataaga cctccaattc
     3781 ctgcgccaat accacagaag acaacgagta ctgcaaatgt taccgcaatg atttctgttt
     3841 tgttcattcc cataatgata actatatgcg tgtatttgtt aaataatctt tacactgtac
     3901 tttccgaaat gcgggctgat ttcctttcgt aacttagtgt cagaatttat cctccatcag
     3961 tacgtatgag tatattgagg tgatcacttt ggatcatggg gctacttctg agaatgcgga
     4021 gcatcaggac atctgttttt gtcctctgca cggtctactg gataccataa gcaaaaagtg
     4081 ggcgctgatg atcatcgcag ttatcggcaa ccacggctcg gcaggattca acgaactgaa
     4141 acgtcagctc tgtaatgtga gttcaaaaac actatcaaat acgctgaagg atcttgagga
     4201 gcatggtctc gtttaccgcc aggtggtgga tcagacacct cccgtggtcc ggtactatct
     4261 cacggtttcc ggatgggaac tgcgtgaact tctgattcca ttgcttgcat gggtgatgaa
     4321 aaacggtggt catgccgatg aggggtgccc aattcacttc aatacaaaga acgtatgata
     4381 aagtggtttt gtacccgggc agtggaccgt tgtctgcgac aggttgatgt tcccctctct
     4441 ccaatataat atccgtattt ttgcgagggt atccaagtgg ccaacggagg ctgactcaag
     4501 atctgctctc aaggagttcg cgggttcaaa tccctcccct cgcactgttt cttggcacgt
     4561 tccatctctt ttatctctcc tcctgccaat atactcacca atgactcctc ggattctcgc
     4621 catatccacc tcaccccgtc gtcacggaaa ctccgagtcg gcacttgata ctgttcttgc
     4681 cgagataggt gatcgatttc ctgcagaaaa agtggtgctt ggcgatctgg caattgctcc
     4741 ctgtaaaggc tgcggagcat gtgaacggct cggccgttgt gttcaggagg atgactttca
     4801 gtccctttcg cagaaaattc ttgatgccga tgtgttgatc ctcgcaacac cggtgtactc
     4861 catgtcggtg tgtgcccagg caaaagctct gatcgatcgc tgtcaggtgt tctggtcgcg
     4921 gaaatatgta ctgcatacct ttgaggaacc agccggaaac aaactcggcc ttttcattgc
     4981 aaccgccgga caaacacgcg acaacatctt tgatcacacc gtacctgtcg cacggtttct
     5041 gtttgatgtg tcgggaatca aaccgaaaca cacaatgctc ctcctcctca aaggtcttga
     5101 taagaaaaat gattatttgg aggatgcatc agcagttgcc aaaacaaaag agatcacagc
     5161 gtctcttctt gcatcccttg aggtagtatg accctgataa ccgcagtaaa tggaagccca
     5221 cgcaagcatg gaaacaccga gacggttctt gatgcttttc ttcgcggggc agaaagtacc
     5281 ggagctgaga cgaacaaact ctctcttgtt gatatcgatc acaaaaactg ccggggctgt
     5341 aatgcctgcc acaagaaagg tgtctgtatt ctcaaggatg atctcacgcc gatctttgat
     5401 gcagtgctcg cctcagatat actcgtgctt gcatccccca tctactcgat gactgtcact
     5461 gcggagatga agtccttcat cgatcgcgga cagtttcttt gggcacagaa gtttgtcact
     5521 aaaacattat cgttctcaga cgaacatctc aaacatcaca ttggcgtgta tctgggaacg
     5581 tccgggcagg agattccgca catctttgat gcggcattcc cggttgtgcg ggcattcttt
     5641 aatgacgcgg gattttcata caccgagaac gttctctttg caggaatgga tcagcacggc
     5701 ggggtgaaag gctggcctga gtcggtggca tttgccgaag cggaaggtaa acggatcgca
     5761 gaactcatct aatctgcatc cggctaattc tattttttca aaaaaaatta tttctgattt
     5821 gatctgtttt gaaggtcagg gtatgcacga atcagccgtc ttctccagat atcctccggc
     5881 gtttcatcca tcaatgcttc tgcgacatgc atctccgcc
//
LOCUS       DALUAJ010000102         2523 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_18135,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000102 DALUAJ010000000
VERSION     DALUAJ010000102.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18135"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="O0X37_02225"
     CDS             <1..930
                     /locus_tag="O0X37_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61777.1"
                     /translation="IYLFQLHTMGYPMPHDTPAESLIAAAEASLAAASSAILQNDLNS
                     AADHLETAVISLLDAGSVLLTSVQQADIAVLDSGLARILRAFAERFGLLPEDHQPILF
                     LLPASVDVLDASTQTAAKTHLPISEVCATVRSVYESKDTDGLELSEEELNTHISAVRL
                     LIDLLLSQRTLADTVFAENGAYDVFSDVASPYLISGAAFVGGSENSETYEAKIPQILA
                     GPVKRLVELLAALLTSAFMSGLILLIIRRILAVRKVRPGMVDNIIFAGSVAALVAANI
                     PALVDGVKQVAAELRNLQQIIEETDGGIEIPIA"
     gene            complement(941..2053)
                     /locus_tag="O0X37_02230"
     CDS             complement(941..2053)
                     /locus_tag="O0X37_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HJK61778.1"
                     /translation="MSLEEEIKAIEDEIRNTKYNKATSQHIGRLKAKLAKIKDDAVTR
                     AMKSAGSGEGYAVKKSGDGTIVLVGFPSVGKSTLLNKLTGAESETGAYAFTTLTVVPG
                     LMEYKGAKIQILDIPGLIAGAAMGKGRGKEVIAVVRTADLIVILGDVFNGVHVDVLMH
                     ELYDAGIRINKPKPDITIKKTGYGGVRLNTVGAVNIDIEEVRAILAESKVMNADVLVR
                     GSNVTQDDIIDAMMGNRMYIPAFIAINKVDLVTEKERQEVENEMREKYGVDPIMISAH
                     AGYNIDKLQEAIYEHLGFVRIYMKPYGEEADMEEPMIMRKGSTIEDICRRLHRDFVDQ
                     FRYAKIWGTSVKHDAAKVGLQHKVEDGDIVTIVTKL"
     gene            2166..2238
                     /locus_tag="O0X37_02235"
     tRNA            2166..2238
                     /locus_tag="O0X37_02235"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2200..2202,aa:Glu,seq:ctc)
     gene            2287..>2523
                     /locus_tag="O0X37_02240"
     CDS             2287..>2523
                     /locus_tag="O0X37_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HJK61779.1"
                     /translation="MYDVIIAGAGPTGSAAARFCAKAGLSTLVLEEHAAIGYPVQCAG
                     LLSNSAFAECGVSDRSVLNTVRGARICGSAGHELS"
BASE COUNT          528 a          680 c          568 g          747 t
ORIGIN      
        1 atttatttgt ttcaactgca tactatgggg tatccaatgc cgcatgatac tcctgctgaa
       61 tcattaattg cagcggctga ggcatctctt gccgccgcct cgagcgccat tctccaaaat
      121 gacctgaatt ccgctgctga tcatcttgaa accgcagtca tcagccttct cgatgccggt
      181 tctgttcttc tcacgtcggt tcagcaggcg gacattgctg ttcttgatag cggtcttgcc
      241 cgtatacttc gcgcgtttgc ggaacgcttt ggtcttcttc ccgaggatca tcagcctatt
      301 ctcttccttc ttcctgcgtc tgtggacgtt cttgacgctt caacacagac ggcagcaaaa
      361 acacaccttc ccatctccga ggtctgtgca actgtgcggt ctgtgtatga atccaaggat
      421 actgatggtt tagaactgtc tgaagaggaa ctgaacacac atatctcagc agtcagactt
      481 ttgatcgatc ttctcttgag ccagcggaca ctggctgata ctgtctttgc tgaaaatggg
      541 gcatatgatg tattctctga tgttgcttcc ccgtatctga tcagtggtgc ggcatttgtt
      601 ggtggcagcg aaaattccga gacgtatgaa gcgaagattc cgcagatact tgcaggacca
      661 gttaaacgtc tcgttgagct tcttgccgcc ctgttaacgt ccgcatttat gtccggcctt
      721 atcctgctga ttatccgtcg tattcttgct gtacgaaaag ttcgtcctgg tatggttgac
      781 aacatcatct ttgcaggatc ggttgccgcg cttgttgccg caaacatccc agcattagtg
      841 gacggtgtaa agcaggtcgc agccgaactg cggaacctgc agcagatcat cgaagaaact
      901 gatggcggta tcgaaattcc gatcgcctaa aaaatatttt ttagagtttt gtgacaattg
      961 ttacgatatc gccgtcctcc accttgtgct gaaggccgac ctttgcggca tcgtgtttta
     1021 ctgatgtgcc ccaaattttt gcataccgga actgatccac aaaatcacgg tgaagtctgc
     1081 ggcagatatc ctcaatcgta ctgcctttcc gcatgatcat cggctcttcc atgtccgcct
     1141 cctctccgta cggcttcata tagatacgca caaacccgag atgttcgtag atggcctcct
     1201 ggagtttgtc gatgttatac cctgcgtgtg ccgagatcat gatgggatca acaccgtact
     1261 tctcacgcat ctcattttcc acctcttgcc gctctttttc tgttacaaga tcaaccttgt
     1321 tgatcgcaat gaatgccgga atgtacatcc ggttgcccat cattgcatca atgatatcat
     1381 cttgggttac attactgccg cggaccagta cgtccgcgtt catcacctta ctctctgcga
     1441 gaattgcacg gacttcctca atatcaatat tcaccgcacc gactgtattc aggcggacac
     1501 cgccatatcc ggtcttttta atggtgatat ccggctttgg tttgttgatg cggattcctg
     1561 catcgtacag ctcatgcatc agcacgtcaa cgtgcacacc gttgaacaca tcacccaaga
     1621 tcacgatcag atccgctgtc cggacaacgg caattacctc tttaccgcgt cccttgccca
     1681 tcgctgcccc cgcaatcaga cctggaatat ccaagatctg aatttttgca cctttgtact
     1741 ccatcagtcc tggaacaacc gtcagtgtgg tgaacgcata tgcaccggtc tcactttctg
     1801 caccggtcag tttattgagc agtgttgatt taccgactga aggaaaacca accagcacaa
     1861 ttgtcccgtc accggatttt ttgactgcgt atccctcacc gctgccggca gacttcattg
     1921 cgcgcgtgac ggcatcgtcc ttgatcttgg cgagttttgc tttcaaccgc ccgatgtgct
     1981 gtgaagttgc cttgttgtat ttggtatttc tgatctcgtc ctcaatggct ttgatctctt
     2041 cttcaaggct catattgtct atacctatac gttttggtat tagatgaaca tatgggggag
     2101 tgtgtgcttt tcaatcggta tctataaata tttcatacgg caacattagt tacgcaagtt
     2161 ctcttgctga tatagtgtag tggccaatca tgtcggcctc tcacgccgac gactggggtt
     2221 cgaatcccca tatcagcact ccttttgttt atcttctctg actgataaca tctctgtatc
     2281 ttttccatgt acgatgtcat cattgccggt gccggtccga ccggttcagc cgccgcacgg
     2341 ttctgtgcca aagctggtct ctccactctg gtactggaag aacatgctgc aatcggttat
     2401 ccagtccagt gtgcgggtct tctctccaac tcggcatttg cggagtgcgg ggtgtctgac
     2461 agatcggttt tgaacaccgt tcgcggtgcc cgtatctgtg gttccgccgg gcacgaactt
     2521 tcc
//
LOCUS       DALUAJ010000103         1879 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_18347,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000103 DALUAJ010000000
VERSION     DALUAJ010000103.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18347"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="O0X37_02245"
     CDS             <1..276
                     /locus_tag="O0X37_02245"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HJK61780.1"
                     /translation="LPTPEEVYEGVISSRIAAHVGDMVKLPKTREADLEMGHARRDLD
                     WERQYAVSLNPKKAREIRDSRMPADTDACTMCGDYCAIKIVQKNFHF"
     gene            360..1115
                     /locus_tag="O0X37_02250"
     CDS             360..1115
                     /locus_tag="O0X37_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61781.1"
                     /translation="MRDLITGTISAFVKAYERRPGTATRWGDPLAGFCDANSDLVQGL
                     KQSVAPDHLMPSDLLPDATVVVVYFLPFTRELAETNRSGTAASPDWARAYAETNALFA
                     ELNDHLVDLIQFHGGRAALPGTAAGFSGTLLKSRWSHRHFAVLAGLGTFGMNNMLLTA
                     SGCCGRVSSFAADIPVTPDKPLSEEFCLYKRSGKCGKCISRCPSGALSPEGFDRERCF
                     AVCMENEARYPGCDVCGKCVTGVPCSFWDSVPF"
     gene            complement(1127..>1879)
                     /locus_tag="O0X37_02255"
     CDS             complement(1127..>1879)
                     /locus_tag="O0X37_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HJK61782.1"
                     /translation="VSAGEALNPEVTRVWEEATGKTIYEAYGQTETVMVIGTFPCIPN
                     KPGSIGKPAPGWCVQLHDEQGNEVPVGEEGKIAIKTKDPSPVGLFKEYLDNPEATSAV
                     FVNGWYYTGDKAVKDEDGYFWFMGRDDDIIKSSGYRIGPTEVESALIEHPAVKESAVV
                     GSPDPIRGVIVKAFVILKDGWKPSDDLVKELQNHVKKTTAPYKYPRAIEFVEDLPKTI
                     SGKVRRVELRDREMKRYQALHHNDPESDSGSQ"
BASE COUNT          379 a          503 c          496 g          501 t
ORIGIN      
        1 ctgccgactc ccgaagaggt gtacgaagga gtgatcagtt cccggattgc tgcccacgtg
       61 ggtgatatgg tgaagcttcc aaagacccgt gaggcagatc tggagatggg tcatgcaaga
      121 cgcgatcttg actgggaacg ccagtatgcg gtttcgctga atccaaagaa ggcacgggag
      181 atccgtgact cacggatgcc tgcggacacg gatgcgtgta cgatgtgcgg tgactactgt
      241 gcaattaaga ttgtgcagaa gaacttccac ttctaatctt ttttatattc tttttcagta
      301 gttccttctc tgctttgttc cggtatgctt atttttctat ccgctgtatg tttttcatca
      361 tgcgggatct gatcaccgga acgatttcag cgtttgtgaa agcatacgag aggcggcccg
      421 gcactgcaac gcggtgggga gatccgctgg cgggattctg cgatgcgaac tcggatcttg
      481 tgcaggggtt gaaacagagt gttgcccctg atcatctgat gccgtcggat cttcttccgg
      541 atgcaactgt ggtggttgtg tacttcctgc cgtttacccg tgaacttgcc gagacgaacc
      601 gatccggtac cgctgcttct cctgactggg cacgggcgta tgccgagacg aatgcactgt
      661 ttgcggagtt aaacgatcat cttgtggatc ttattcagtt tcacggaggc agagctgctt
      721 tgccgggcac ggctgccggg ttttccggta ctcttctgaa aagccggtgg tcgcatcgcc
      781 attttgccgt ccttgccggt cttggaacgt ttggtatgaa caatatgctt cttaccgcat
      841 ccggctgctg cggcagggtg agttcatttg cggcagatat tccggttact ccggataagc
      901 cactctctga ggagttctgt ttgtacaaac gatccggaaa atgcggcaaa tgtatctccc
      961 gctgtccgtc tggagcactt tcaccggagg gttttgatcg ggaacggtgt tttgcggtgt
     1021 gtatggaaaa tgaagcgcgt tatcccggat gtgatgtgtg cgggaagtgt gttactggtg
     1081 tcccatgttc gttttgggac tcagtcccct tttaatctga aaaaagttac tgggatccgg
     1141 agtctgattc cggatcattg tggtgcagag cctgataccg cttcatctcg cggtcacgaa
     1201 gctcaactct tctgaccttg ccggagatcg tcttcggcag atcctccaca aactcgatcg
     1261 cacgcgggta cttgtacggt gcggtcgtct tcttcacatg attctgcagc tctttaacaa
     1321 gatcatccga cggcttccag ccgtccttca gaatcacaaa tgccttcaca atcacaccgc
     1381 ggatcggatc agggcttccc acaacagccg actccttcac tgccgggtgc tcgatcagtg
     1441 ccgactccac ctcagttgga ccaatccgat agccggaact cttgatgata tcatcatcac
     1501 gtcccataaa ccagaaatag ccgtcctcat cctttactgc tttatctccg gtgtaatacc
     1561 agccgtttac aaacaccgcc gacgtggcct cggggttgtc cagatactcc ttaaagagac
     1621 caaccggact tggatccttc gtcttgatcg cgatctttcc ttcctcgccg acaggaacct
     1681 cattaccctg ttcatcatgc agctgcacac accatcccgg agcgggcttg ccgatggatc
     1741 ccggcttgtt tggaatacag gggaaggtac caatgaccat cacagtctcg gtctggccgt
     1801 atgcctcgta gatcgtcttt ccggttgcct cttcccagac acgggtaacc tccgggttca
     1861 gtgcctcacc ggcagacac
//
LOCUS       DALUAJ010000104         2954 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_18400,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000104 DALUAJ010000000
VERSION     DALUAJ010000104.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18400"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="O0X37_02260"
     CDS             complement(<1..449)
                     /locus_tag="O0X37_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61783.1"
                     /translation="MMQVEQGFISALTLKEHTVHHGADLEGVEVTTADGCCFTIESEH
                     PLPEFFVSEERLRERLMHELTLVHGIGGMREQICRRRGIQTLTDLQRTNWKVEAREVA
                     DILLNGTPRDIIQLFREKGRGADPLLVGLGAAVPKEDLLFFDIETLG"
     gene            482..1252
                     /locus_tag="O0X37_02265"
     CDS             482..1252
                     /locus_tag="O0X37_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HJK61784.1"
                     /translation="MTVSGFLIDLDGVVYINGEPIPGAVPALQELKRRGIPFRFVSNN
                     THRSRETIQARLARFGVDIPVEWIFTPLTAAISYLRQAGATSCWLLGSPDAAKELTDA
                     GINPADPNASHVLIGDLSDVLGYEMFVAGFRVLMRNHAELLALEHDRYFKGSDGLLLS
                     AGAFVAALEFAADTSAKLLGKPSAAFFQAALSSLGLPPEEVVMIGDDPRSDIGGAATV
                     SVRGVLVLTGKFDGVLPDGVVAPWKILPGLADVLDLTQ"
     gene            complement(1242..1397)
                     /locus_tag="O0X37_02270"
     CDS             complement(1242..1397)
                     /locus_tag="O0X37_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61785.1"
                     /translation="MTSIALTSMAERAAQYTVIQRKNQLSKLCWYAAGTPGNMIPSLK
                     DPTYLIA"
     gene            1526..2254
                     /locus_tag="O0X37_02275"
     CDS             1526..2254
                     /locus_tag="O0X37_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK61786.1"
                     /translation="MLEISNLHVEVGGREVLHGVNLKINDGETHVLLGPNGSGKTTLL
                     RTIMGFGSSQVTSGKILFNGVDVTEKPVHERAQLGMGIMFQRPPTISGLKLGKFLTAT
                     TKIDEERIPSLAERMHMTKFLDRDVNAGFSGGEIKRSEILQLMVQNPSFVMLDEPESG
                     VDVENMALLGNAAASLLEKDQHMINRTKSGLIITHTGYILDYIEADVGHVLIDGVMRC
                     SGNPREILRSVKTSGYKECIACQK"
     gene            2242..>2954
                     /locus_tag="O0X37_02280"
     CDS             2242..>2954
                     /locus_tag="O0X37_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HJK61787.1"
                     /translation="MSEMKGFDSISTEDKERLALTGIQTDSMEGRAGSFLLVNDHVLH
                     AGSNAEGVEVMMIDKALVKYAWLADYCWNVVPKDKDQYTQYVADHPQRGYVIIARKGA
                     KTTFPLQSCMFLAGDAIQAVHNIVIAEEGAEVHLIAGCASSMKTKEGAHYGINEIYVG
                     KNAKVTSTMIHTWGEEIEVFPRTASVVEEGGTFISNYVCMKPTKMVQMYPTAHLRGKG
                     AVSRFSSVIVAGPGSHIDAG"
BASE COUNT          715 a          794 c          755 g          690 t
ORIGIN      
        1 ataccaagcg tctcaatatc aaagaacagc agatcctcct ttggcactgc cgccccaagt
       61 ccaaccagaa gcgggtcggc accgcgtcct ttttcccgaa acagctggat aatatctcgc
      121 ggagtaccat tcagaagaat atctgcaacc tcccgcgcct caaccttcca gttggtacgc
      181 tggagatcag taagcgtctg tatcccgcgt ctgcggcata tctgctcgcg catccctcca
      241 atcccatgca caagtgtcag ctcgtgcatc agccgttcgc gaagtctctc ctcggataca
      301 aaaaactccg gcagaggatg ttcggactca attgtaaagc agcatccgtc cgcggttgtc
      361 acttcaactc cttccagatc agccccatgg tgtacagtat gttccttgag ggtgagggcc
      421 gaaatgaacc cctgctctac ctgcatcatt ttttcattct ctctccaata ttgatactca
      481 tatgacggtt tcggggtttt tgatcgattt agatggtgtt gtgtatatca acggcgagcc
      541 gattcccggc gcggttcccg ctctgcaaga gttgaagcgg cgcggcattc cttttcgatt
      601 cgtttccaac aacacgcaca gaagcaggga aaccattcag gcacgtcttg cacgattcgg
      661 cgtggacatc ccggttgaat ggatctttac cccccttact gccgcaatct cctatctccg
      721 gcaagcagga gcaacctcat gctggctgct cggaagtccc gacgccgcaa aggagcttac
      781 agacgctgga attaatcccg cagacccaaa cgcgtcacac gttctgatcg gcgacctgag
      841 cgatgtgctg gggtatgaga tgtttgttgc aggcttccgt gtattgatgc gtaaccatgc
      901 cgaactgctt gcccttgaac acgatcgcta cttcaaagga agtgacgggc ttctgctctc
      961 agcaggtgca ttcgtagcag ctctcgagtt tgccgcagac acatccgcca aactgctggg
     1021 aaaaccatcc gcagcatttt tccaggcggc actctcttcc cttggtcttc ctccggaaga
     1081 agtggtcatg ataggtgatg atccgcgttc ggacatcggc ggtgcagcca ctgtatccgt
     1141 gcggggtgtt ctcgttctca ccggcaagtt tgacggagtg cttcccgacg gtgtagtagc
     1201 tccctggaaa atactcccgg gacttgcgga tgttcttgat cttacgcaat aaggtaggtc
     1261 ggatccttca gagacggaat catgttgcct ggagttcccg cagcatacca gcagagcttg
     1321 gataactgat tcttgcgctg aattacagta tactgcgcag cacgttctgc cattgaggtt
     1381 aatgcgatgc ttgtcatgat caatcatcag aatggaggaa tataaacccc ggaccgcgcg
     1441 tactgaaagt gccctcccat atggaaaacg taatcaaaca cgtatcttta caaccctcca
     1501 caacaaatta catagcagat tttccatgct tgagatcagc aatcttcatg tggaagttgg
     1561 cgggcgtgaa gtccttcacg gcgttaatct caaaattaac gatggtgaga cccacgtact
     1621 tctcggaccg aacggatcgg gaaaaaccac ccttctgcgt accatcatgg gatttggcag
     1681 ttcccaggtc accagcggca aaatactttt taacggcgtt gatgtcaccg aaaaaccagt
     1741 gcacgaacgg gctcagcttg gtatgggtat tatgtttcag cgtcccccca ctatctccgg
     1801 cctgaaactt ggtaagtttc tgaccgcaac aaccaagata gacgaggagc gtatcccttc
     1861 gctggcggaa cgcatgcata tgacaaagtt cctcgatcgt gatgtcaatg ccggattctc
     1921 cggcggcgag atcaaacgca gtgaaatcct ccagctcatg gtgcagaacc cctctttcgt
     1981 catgctggac gagccggaaa gcggtgttga tgtcgagaac atggcactgc tcggaaatgc
     2041 tgcggcaagt ctgctggaaa aagatcagca catgattaac cgcaccaagt ccggcctcat
     2101 tatcacgcac accggctaca tcctcgatta tatagaagcc gatgtcggcc acgtcctgat
     2161 cgacggcgtg atgcggtgca gcggaaaccc gcgtgaaatt ctccggagtg taaagacatc
     2221 cggttacaag gagtgtatcg catgtcagaa atgaaaggat ttgactccat cagtaccgag
     2281 gataaagagc ggctggcact gaccggcatt cagacagatt ccatggaggg acgtgccggc
     2341 agtttccttc ttgttaatga ccatgttctc cacgcaggct caaatgccga gggcgttgag
     2401 gtcatgatga tcgacaaagc actggtgaag tatgcatggc tggcagacta ctgctggaat
     2461 gttgtaccaa aagacaaaga tcagtacacg cagtacgttg ccgatcatcc ccagcgcggc
     2521 tatgtaatca tcgcccgcaa aggtgccaag accaccttcc ccctgcaaag ttgtatgttc
     2581 cttgccgggg acgccattca ggcagtgcat aatattgtaa ttgccgagga aggggccgag
     2641 gtccatctga ttgccggatg tgccagttct atgaagacaa aagaaggtgc acactacggt
     2701 atcaatgaga tctatgtcgg caagaacgca aaggtgacct caaccatgat ccacacctgg
     2761 ggtgaggaga tcgaggtgtt tccgcggaca gcctctgttg ttgaagaggg aggaacgttc
     2821 atctcaaact atgtctgcat gaagccaacc aagatggtgc agatgtatcc taccgcacat
     2881 ctccgcggga agggtgcggt ctcccgtttc tccagcgtga ttgtggcagg gcccggatca
     2941 cacattgatg cggg
//
LOCUS       DALUAJ010000105         4741 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_18435,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000105 DALUAJ010000000
VERSION     DALUAJ010000105.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18435"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="O0X37_02285"
     CDS             complement(<1..801)
                     /locus_tag="O0X37_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015655 - alanine:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0032328 - alanine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HJK61788.1"
                     /translation="MSVFETVNAVLTSAVAVVNEYIWYLAFIMLGCMGLYFTWKTKGV
                     QLSMLKESCRLSFHGMKASDVLGGKNAVSSFQAFCVSMGARVGVGNISGVGMAFIMGG
                     PGAIFWMWVFALIGAATSFVECTIGQIYQEKGADGLFHGGPAYYIKNALGKGKFAIFV
                     ALLVIITYPICFTQVQANTITDAFVTAFNIDPLIMAVIVTVISAIVILGGIARVARVS
                     SWLVPIMAVVYLIIALIVIAMNFTKIPEMFSVILGCAFGFQQIGGAALG"
     gene            complement(894..1211)
                     /locus_tag="O0X37_02290"
     CDS             complement(894..1211)
                     /locus_tag="O0X37_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61789.1"
                     /translation="MGSEVYQAQVLRAFFDTITGTDRNLTRIYMCVISLAKLRGEDPE
                     KLRFLVEQMRASKEKRELSIDILDYMAEAANSLEPWAGQSAFGITTPVKSEDFGGISM
                     DSF"
     gene            complement(1254..2426)
                     /locus_tag="O0X37_02295"
     CDS             complement(1254..2426)
                     /locus_tag="O0X37_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HJK61790.1"
                     /translation="MLLLALAGKPNCGKSTFFKSLTLANVEIANYPFTTIDANKGVAY
                     VRSPCPCKELGLDNCTSCVDGVRFIPVELLDVAGLVPDAHLGRGLGNQFLDNLREADG
                     IIQVVDASGSTDAEGNPVDIGTRDPREDVEFLRYEFAMWMAGIVDKHLAKLVRQAQGK
                     EAVLIDLLAGALAGLRINAIQIREAVDETGINLAKATPEEIEQMCEVLLRVSKPMIIA
                     ANKADQASEANLASLKELGAVPTIAAGELALKSAAQAKILRYLPGDGTFAPIEGAKLS
                     APQLKALTMIAENMKKFGGTGVQEALNTLVFEEIGMIVVYPVEDDNKCCNAKGVVLPD
                     AFLMPKGSTPKDLAFRVHTDIGNGFLYAVDARTKMRIKDSTELKSGDVIRIVSTAK"
     gene            complement(2680..3615)
                     /gene="cas1"
                     /locus_tag="O0X37_02300"
     CDS             complement(2680..3615)
                     /gene="cas1"
                     /locus_tag="O0X37_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HJK61791.1"
                     /translation="MSDAVPWVTVWGYGADIRATRRTLTIREGPVRRSYPLSSIRHLL
                     IAGGHTLETAAVSHLMDNNVPVSFFDIHGEPVGTIRPSGHSAYPLRPAQKSIPVHRFA
                     MSVIITSLKSRMGYLHELGSRRDDGLYYKGELEILTDASHELEYLITLPELSRVFLLT
                     RTMYYEILSRVADPRLGYRRREKPPYLDPINAMLAHGYAVLYASTSVSVAGAGLDPEI
                     GSLYGNVIPPGKNRSACVMDIMEPLMTPMVDRVVMDMAQEGVIAGRYEISSRCLLSEK
                     LMEECNRRFAESINSDDIDRAVSEYAAAITTSTSA"
     gene            complement(3612..4253)
                     /locus_tag="O0X37_02305"
     CDS             complement(3612..4253)
                     /locus_tag="O0X37_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HJK61792.1"
                     /translation="MFMSRHNEIIYHMGVHVFWDSRVPIGLSRPVTEELSGVLEMPVS
                     RIDNGTFPLEGFDPLRQQCDAVKILTKLDIFRRRYPQLFKPDDVDIEFYNKFNHIHEK
                     VLLVTPVDLFEPLADFVFGLAYPALGVAVVSPARLTNEYYGRHPDDDALIDRIVKESA
                     HEIAHLFGLEHCSNSNCIMYCPRNLDDLDRKRKYFCGKCRIQLNGQNEGDLFQ"
     gene            4283..4675
                     /locus_tag="O0X37_02310"
     CDS             4283..4675
                     /locus_tag="O0X37_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0146 family protein"
                     /protein_id="HJK61793.1"
                     /translation="MSVYQIERYVGTYIRDHYSSAVEIGFGGKTVAAEIIQDAGIPIL
                     CTDIHSYQTTVVPAVVDDVFSPTHSYYQGKDVLYAIRPGCEMIPPMIALAEQIGAELI
                     VYHLGFELYENGGEVLDLGDILLHRYVK"
BASE COUNT         1334 a         1147 c         1114 g         1146 t
ORIGIN      
        1 accaagggct gcaccaccaa tctgctggaa gccaaatgca caaccaagga tgacggagaa
       61 catttccgga atcttggtga agttcattgc aatgacaatc agtgcaatga tcaggtacac
      121 aaccgccata attgggacaa gccatgagga tacccgtgca acacgagcaa ttccaccaag
      181 aatgacgatt gcagagatca ctgtcacaat aactgccata atcagaggat caatgttgaa
      241 cgctgtgaca aacgcatcag tgatggtgtt tgcctgcacc tgcgtaaagc agatcggata
      301 cgtgataatg acaagaagtg caacaaagat agcaaactta cctttaccaa gggcattctt
      361 gatgtaatag gcaggtccac cgtggaacag accatcggca cctttctctt ggtagatttg
      421 accaattgta cactcaacaa aactcgttgc tgcgccaatc agagcaaaga cccacatcca
      481 gaagatagca ccaggtccac ccatgatgaa ggccattccc acaccagaaa tgttacctac
      541 accaacacgg gcgcccatgc tgacacaaaa cgcctgaaaa gaagatacag catttttacc
      601 accaagaaca tcacttgcct tcattccatg gaaggaaaga cggcaggact ctttgagcat
      661 actaagctgc acaccctttg ttttccaagt gaagtacaac cccatacaac cgagcatgat
      721 gaatgcgagg taccagatgt actcgttcac caccgctact gctgaggtga gtactgcgtt
      781 gacagtctca aacacactca tgagataaat cttttctcat cgtaacatat taacgtgttg
      841 taacgtatcg ccacaacaaa aaatgaaaag aagttcctaa cgtatggaac tatttagaag
      901 gaatccattg agattccgcc gaaatcctcg gacttcacag gggttgtgat gccgaatgcg
      961 gactgccccg cccaaggctc aagagagttg gctgcttctg ccatataatc aagaatatcg
     1021 atggagagtt cgcgcttttc tttgcttgcc cgcatctgct caactaaaaa tctgagcttt
     1081 tcagggtctt caccccggag tttagcaagg gatatcacac acatgtagat acgggtcaga
     1141 ttcctgtcgg taccggtaat ggtatcaaag aatgcccgaa ggacctgtgc ctgatacacc
     1201 tcgcttccca taattactct aactttgcgg ctgataggta atatagattg ccgtcatttt
     1261 gcggtactga cgatccgaat aacgtcaccg gatttcagct cggtactgtc tttgattctc
     1321 atttttgtac gggcgtcgac agcatagagg aatccgtttc cgatatcagt atgcaccctg
     1381 aatgcaagat ctttgggggt actgcctttt ggcatgagga aggcatcagg cagaacaaca
     1441 ccttttgcat tgcagcattt attgtcatcc tcaacgggat agacaacaat cataccgatc
     1501 tcctcaaaga caagagtgtt gagtgcctcc tgcacaccgg ttccaccgaa ctttttcatg
     1561 ttctccgcaa tcatggtaag cgcttttagt tgcggtgccg aaagttttgc accttcgatc
     1621 ggcgcaaacg taccatctcc cgggagataa cggaggatct ttgcctgtgc tgcagactta
     1681 agggcaagct cacctgctgc aatagtgggg acagcaccaa gttctttgag tgatgcaagg
     1741 tttgcctcgg atgcctgatc cgctttgttt gcagcgatga tcatgggttt tgaaacacga
     1801 agcagaactt cgcacatctg ttctatctcc tcaggtgttg cttttgcaag gttgatgccg
     1861 gtttcatcca ccgcttcacg gatttggatt gcgttgattc gtaaaccggc aagtgctcct
     1921 gccagaagat cgattaacac agcctctttg ccctgtgcct gacgaacaag tttggcaaga
     1981 tgtttgtcaa caatccccgc catccacata gcaaactcgt atctgaggaa ctccacatct
     2041 tcacggggat cgcgggtacc aatatcaacc gggttacctt ctgcatcagt acttcccgat
     2101 gcatcaacaa cctggataat accgtcggcc tcccggagat tatcaaggaa ctgattgcca
     2161 agacctctgc caaggtgtgc atcgggaaca agacctgcta catccaggag ttcaacaggg
     2221 atgaatcgaa caccgtcaac acaggaggtg cagttgtcca gaccaagttc tttacagggg
     2281 cagggggacc gcacataggc gacacctttg tttgcgtcaa tggtggtgaa cgggtagttg
     2341 gcaatctcaa cgtttgcaag agtcagcgac ttaaaaaatg tcgattttcc gcagtttggt
     2401 tttcccgcaa gggcaagaag caacatgagt agaatctcct gaacttttcc ggcggagtac
     2461 gatatttttc ctgccgggca tattttcaag aaatgattag gttatgcaga cagatgtatc
     2521 cttcggaatt cctggggaca atgatccgac acattttgat caggaaccac cgatgattgc
     2581 agatgggttt gcgaatagga ggtcttgcag ttggctccgc tctgatgact gtttaaaaga
     2641 cctgcaaact cagtcgcact gcatgaagaa ccgggaagct taggcactgg tactggttgt
     2701 gatcgcagcc gcatactctg aaaccgcacg gtcaatatca tctgagttaa tagactctgc
     2761 aaaacggcgg ttacattctt ccatcagttt ttccgaaaga aggcaacgcg aacttatctc
     2821 atatctcccg gcgatgacac cttcctgtgc catatccatg acaacacgat cgaccatcgg
     2881 tgtcatcaaa ggctccatga tgtccataac acatgcactg cggtttttcc ccgggggaat
     2941 gacatttccg taaagagaac cgatctccgg atcgagtcca gcaccggcaa cagatacaga
     3001 agtacttgca tagagaacag catacccgtg agcaagcatt gcgtttatgg gatccagata
     3061 cggaggtttt tccctgcgcc ggtaaccaag cctaggatct gcaacacgcg agagaatctc
     3121 atagtacatg gtacgagtaa gcagaaagac tctggatagt tcagggagag taataagata
     3181 ctctaattca tgggatgcat cagtgagtat ttccagttct cccttatagt acaggccgtc
     3241 atcacggcgc gaaccgagtt cgtggagata ccccatacgc gatttcagtg aagtgataat
     3301 gacggacatt gcaaaccggt gaaccgggat acttttctgt gcgggtctaa ggggatatgc
     3361 actatgccca gacggacgga ttgttccaac cggttctcca tgaatatcaa agaaagaaac
     3421 ggggacatta ttatccatca gatgtgaaac cgcagctgtc tcaagtgtat gaccgcctgc
     3481 aatgagcaga tggcggatgg aggaaagagg ataggatctc cgcacaggtc cctcgcggat
     3541 agtgagggtt ctccgagttg cacgaatatc tgcaccgtat ccccagacag taacccacgg
     3601 aactgcatca ctcattggaa caaatctccc tcgttctgac cgttcagttg gattctgcac
     3661 ttgccacaga aatattttcg ttttcgatca agatcatcaa gattgcgcgg acagtacatg
     3721 atacaattag agttgctgca gtgctccaga ccaaagaggt gagcaatctc atgggcactc
     3781 tccttaacga tacgatcaat tagtgcatcg tcatcaggat ggcggccgta atactcattc
     3841 gtcagtcggg ctggagacac cacagcaact ccgagagcag gataagcaag accaaataca
     3901 aaatcggcaa gaggttcaaa cagatctacc ggagttacca gcaggacttt ttcatgaatg
     3961 tggttgaatt tattgtaaaa ctcaatatca acatcatcag gcttaaagag ctggggatat
     4021 cgacggcgaa aaatatcaag tttcgtgaga atttttaccg cgtcacactg ctggcggagc
     4081 ggatcaaatc cttcaagcgg aaaagtaccg ttgtcgatac gggacacagg catctccagt
     4141 acacctgaga gctcctctgt gacaggtctg ctcagaccaa tcggaacccg gctgtcccaa
     4201 aaaacgtgta ctcccatgtg gtagataatt tcattgtgac gggacataaa catattgaac
     4261 acgtatcaaa aggagaaggc gtgtgtcagt atatcagatt gagagatacg taggaaccta
     4321 catccgcgat cattactcat ccgcagttga gatcggattc ggggggaaaa cagtcgcagc
     4381 agagataatt caggatgcgg gaattccgat tctgtgtact gacattcact catatcagac
     4441 gaccgttgta cctgctgttg tagatgacgt gttttccccg acacattcct attatcaggg
     4501 aaaagatgta ctgtatgcta tccgaccggg atgcgagatg attcctccca tgatagcact
     4561 tgcagaacaa atcggagccg aactgattgt gtatcatctc gggtttgagc tgtatgaaaa
     4621 cggaggagaa gttctggatc ttggagatat tttgctgcac cggtatgtga aatgaaccaa
     4681 acgaaacatg atacccgcac cttcttttgg caacactaaa accattacac gggagacaag
     4741 c
//
LOCUS       DALUAJ010000106         2594 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_18466,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000106 DALUAJ010000000
VERSION     DALUAJ010000106.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18466"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /gene="pheT"
                     /locus_tag="O0X37_02315"
     CDS             complement(<1..709)
                     /gene="pheT"
                     /locus_tag="O0X37_02315"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HJK61794.1"
                     /translation="MPVVKLNNAYLERLTKTDIKTIRDNLPMMGSEVEREEEDQTDVQ
                     FFPNRPDLYSAEGTARAMRGYLGIETGLPIYTVKPSGISFSIDPKLANIRPYLGSAII
                     RNVHMDNAMIESLMGLQESLHWAVGRGRKKVAIGVHDLDKIEGPFSYVAADRSTTFVP
                     LDYDISMTMDQILAEHPKGKAYAKIVEYLPLFPLIHDAKGRVCSFPPIINGELTRVTE
                     NTRNILLDVTGIEPRAVM"
     gene            complement(712..2136)
                     /locus_tag="O0X37_02320"
     CDS             complement(712..2136)
                     /locus_tag="O0X37_02320"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HJK61795.1"
                     /translation="MDLTINEKRVLASLAELKSADAAVLAEKLDAPADAVVQWAHLCA
                     DRGLAVLEKNVVETAILTEEGKKYAAAGLPERQVLATINGPTPMGELTKHPLSKIAIG
                     WLRKKNWITIDKGVVTVNGEVPAGEDELALKNPVAGTPGCKELVKRGLAEITETVSWT
                     ISITPAGTEIAAAGLDLREEVGTLSREQILSGEWKSLPLRRYSVDKLPKKIYGGRVHP
                     NQQILDEIRALLFEMGFSEFHGSIVQNAFWNFDSLYQPQDHPAREMQDTFHLAEEIDL
                     PDGWEKIRNIHMTGGDTGSTGWGGEWSPKVARKCVLRTHSTSLSIQHLAKNPNPPVKA
                     FSISRVYRRETIDPTHLPEFEQLEGIVMDKDLTFGHLLGFFKEFFGRMGFESVRFRPG
                     YFPYTEPSVEPEVWVDGLGWVELGGAGVFRKEVTAPWGIDCPVLAWGLGVSRVSMLRM
                     GLKDLRQLYRSDIDWIRASPVRGV"
     gene            complement(2139..>2594)
                     /locus_tag="O0X37_02325"
     CDS             complement(2139..>2594)
                     /locus_tag="O0X37_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HJK61796.1"
                     /translation="AGHTFEETDAIVREVEREFGGFGFLLPSATYHSFLQGLQGGKMS
                     SSVPDSLVWFDDPDKDVKKKIMGALTGGRQTLEEQKKLGGEPEKCSIYQLNRFHMQED
                     DKELSEMCRACKAGELMCGTCKKETLERVRNFLREFKEKRDEVAHMVEW"
BASE COUNT          597 a          737 c          604 g          656 t
ORIGIN      
        1 ccatcaccgc acgcggttca ataccggtta cgtccaggag gatgtttctg gtattttctg
       61 taacacgggt cagctccccg ttaataatcg gcgggaatga acagactctg ccctttgcat
      121 catggatcaa cgggaacagc ggaagatact ccacaatttt tgcataggcc ttgcctttgg
      181 gatgctcggc aagaatctgg tccattgtca ttgagatgtc ataatcaagt ggaacaaacg
      241 tcgttgaccg atcagccgcg acataagaga acggaccttc aatcttatcg agatcgtgta
      301 cgccgatagc aaccttcttt cttccgcggc caactgccca gtgtaacgac tcctgcagac
      361 ccatcaggga ttcgatcatc gcattgtcca tgtgaacatt gcgaataatc gcagagccga
      421 gatacggacg aatgtttgca agtttgggat caatggagaa tgagatccct gatggtttca
      481 ctgtataaat cggaagaccg gtctcgatgc cgagatatcc ccgcattgca cgtgctgttc
      541 cttctgcact gtagagatca ggccggtttg ggaagaactg aacatcggtc tgatcctcct
      601 cctcacgctc aacctcggat cccatcatcg gcagattgtc acggattgtc ttgatatccg
      661 tttttgtcag ccgttccaga tatgcattat tgagttttac caccggcata gttacacccc
      721 cctgactggg cttgcacgaa tccagtcaat atcgctccgg tagagctgac gaagatcctt
      781 gagacccatc ctcagcatgg atacgcggga taccccgagt ccccaggcaa gtaccgggca
      841 atcgataccc cagggagcgg taacctcttt gcggaacaca ccggctcctc ccagttcaac
      901 ccagccgagt ccgtcaaccc agacctcagg ctccacggac ggttcggtat aggggaagta
      961 acccggccgg aaccgcaccg attcgaatcc cattcttcca aagaactctt tgaaaaatcc
     1021 aagcagatgg ccgaatgtca gatctttgtc catcacgatt ccttccagct gctcaaactc
     1081 cggtaggtgg gttggatcaa tcgtctcgcg gcggtacaca cgcgaaatag agaaagcctt
     1141 caccggagga ttcggatttt tagccaggtg ctggatggaa agtgaggtac tgtgagtccg
     1201 cagaacacat ttgcgggcaa ccttgggact ccattcacca ccccatccgg ttgatcctgt
     1261 atcaccgccg gtcatatgga tgttcctgat cttttcccag ccgtccggca gatcgatctc
     1321 ctctgcaaga tggaatgtat cctgcatctc acgggcggga tgatcctgcg gctgatacag
     1381 agagtcgaag ttccagaacg cattctgtac aatcgatcca tggaactcgg aaaatcccat
     1441 ctcaaacagc agtgcgcgga tctcatcaag gatctgctgg ttgggatgca cgcgtccccc
     1501 atagatcttc tttggaagtt tgtccacact gtaacgacgc agaggtaaac tcttccactc
     1561 gccggacaga atctgttcac gggaaagtgt tcctacctct tcgcggagat cgagacccgc
     1621 tgcagcgatc tcagttccgg cgggggtaat ggatattgtc catgaaaccg tctcagtgat
     1681 ctcggcaagg ccacgtttta ccagttcttt gcacccaggg gtccctgcaa caggattttt
     1741 cagtgcaagc tcatcctccc ccgcgggtac ttcgccgtta accgttacaa ctcctttgtc
     1801 gatggtgatc cagtttttct tacgcagcca gccgatcgca atctttgata acggatgttt
     1861 cgtaagttct cccatcggtg tgggaccatt gatagttgca agaacctgcc gctccggaag
     1921 gccggccgct gcatattttt ttccttcctc tgtcaggata gccgtctcta caacattttt
     1981 ctcaagtacg gcaagaccgc ggtcggcgca aagatgtgcc cactgcacca cagcatcagc
     2041 cggggcatcc agtttttctg caagtactgc agcatccgca gatttcagct ctgcaaggct
     2101 tgcaagtaca cgtttttcat ttattgtcag atccatagtt accactccac catatgtgca
     2161 acctcatcgc gtttctcttt gaactcccgt aagaagttcc ggacccgctc aagtgtttct
     2221 ttcttgcagg ttccgcacat tagttctccc gctttacagg cacggcacat ctcggaaagc
     2281 tctttgtcat cctcctgcat gtggaaccgg ttcagctgat aaatcgaaca tttctccggt
     2341 tcacccccaa gttttttctg ttcctcaagc gtctgccttc ctccggtgag tgcgcccata
     2401 atcttctttt taacatcctt gtcaggatca tcaaaccaga ccagactgtc cggaacactt
     2461 gaagacatct ttccgccctg cagtccctgt aaaaagctgt gatatgtggc agacggtaac
     2521 aggaagccaa aaccgccaaa ttcacgctcg acctcccgga cgatcgcatc ggtttcttca
     2581 aatgtgtgtc ctgc
//
LOCUS       DALUAJ010000107         3029 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_19426,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000107 DALUAJ010000000
VERSION     DALUAJ010000107.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19426"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(103..1401)
                     /gene="purD"
                     /locus_tag="O0X37_02330"
     CDS             complement(103..1401)
                     /gene="purD"
                     /locus_tag="O0X37_02330"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HJK61797.1"
                     /translation="MNMRILVVGGGGREHAITLALSKNTKTDLYCVMGKKNPGIAKLC
                     REVLIHPETDADAVLAFAKEHDIDYAVVGPEAPLAAGVSDALVAAGIGCVGPSKAAAR
                     IETDKGFCRSLMQKYGIDGCPAYKLCKNPEEAAEYIRSYTGDLAVKPTGLTGGKGVKV
                     MGEQVDREGAVAYAMTLRDQTIILEERLLGEEFTLMAFVDGKTLVPMPLVQDHKRAFD
                     GDVGPNTGGMGSYTLADHTFPFVTEADYNAAFAIMQETVAALAKEGCPYKGVLYGQFM
                     NTADGPKVIEFNARFGDPEAMNVLTLLDSDFVTIAEHIVAGTLTPEDVSFKKQATVCK
                     YIVPKDYPEHPHAGDPITLGSHENTILYYANVVEENGVLQTQTSRTMAFVGVGADLAE
                     AETAAETACRNVSGNVRHRADIGTEALFAKRIAHMKELRG"
     gene            complement(1427..1939)
                     /gene="pyrE"
                     /locus_tag="O0X37_02335"
     CDS             complement(1427..1939)
                     /gene="pyrE"
                     /locus_tag="O0X37_02335"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HJK61798.1"
                     /translation="MENPVLDLLIQYKAVEFGDFTLASGVKSKYYIDVKTAIMQPALL
                     GEIAKEVAERYTFDAVAGVAVGGVPLAVAVSLASGKPCAVIRAAAKDHGKSQMVIGDV
                     AGKHVLLIEDVTTSGGSSKYGIDELRKAGAIVDSVVVVVDRDSGAEALLAAEGVTLHP
                     LVRASELLNR"
     gene            complement(2089..2646)
                     /locus_tag="O0X37_02340"
     CDS             complement(2089..2646)
                     /locus_tag="O0X37_02340"
                     /EC_number="2.7.7.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol
                     synthase"
                     /protein_id="HJK61799.1"
                     /translation="MIVETILAVVTTFVAAFWIMVPAYVANAGAVPFGGGTPVDFGKC
                     AKDGRRYLGDGKTWRGLIGGIVVGILFGLLQIFLAQTFHWDWLPEQTIVTITALAAGA
                     LLGDMVKSFFKRRAGKDRGEKWPIADMYDLVAGSLVLLAVFAWPWIAANMTIWIFIAI
                     LIWTPLLHRGANIIGYLIGIKQVPW"
     gene            2748..>3029
                     /locus_tag="O0X37_02345"
     CDS             2748..>3029
                     /locus_tag="O0X37_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJK61800.1"
                     /translation="MKIIKETKGMCPVCGKLLPATVTEEDGAIWIRRTCPDHGEFRSL
                     YWSDAEMYHRFEQYDREGRGVYNPNTNTPGECTQRCGLCSHHKSGTLLAN"
BASE COUNT          724 a          830 c          688 g          787 t
ORIGIN      
        1 gaggaactcc cgcataccgc tggcgtttca gccgtgcagc gagtgtaagg atgtcgtcgt
       61 actcttcgca cgagagatcc gtaattgaga taaagtcctt tttcatcccc gcagctcctt
      121 catgtgcgca attcttttcg caaacagtgc ctctgttccg atatctgcgc ggtgacggac
      181 attgccggac acattcctgc atgcggtctc tgctgcggtc tccgcctcgg caagatcggc
      241 accgactccg acaaatgcca tagtccggga tgtctgtgtc tgcagtactc cgttctcctc
      301 caccacgttt gcatagtaga gaatcgtatt ttcgtgtgat ccaagtgtga tcggatcgcc
      361 tgcatgcgga tgctcggggt agtcttttgg tacaatgtac ttgcagaccg ttgcctgttt
      421 tttgaaggag acatcctccg gggtgagcgt tccggccacg atgtgttctg caatggtaac
      481 aaagtcagag tccagaagcg tcaacacatt cattgcttcc ggatccccaa acctggcatt
      541 gaactcaatg acttttggtc cgtctgctgt attcataaac tgcccgtaca gtactccttt
      601 gtaaggacaa ccttcctttg caagtgctgc aacagtctcc tgcatgattg caaacgctgc
      661 gttgtagtcc gcttcggtta caaacgggaa ggtgtgatct gcaagcgtat acgatcccat
      721 cccgccggtg ttcgggccga catccccgtc aaacgcacgc ttgtgatcct gcacaagagg
      781 catggggacc agtgtctttc catccacaaa cgccattaac gtaaactcct caccaagaag
      841 ccgttcttcc agaatgatcg tctgatccct gagcgtcatc gcataagcca ctgccccttc
      901 gcggtcaacc tgctcgccca tcaccttgac tcccttgccg ccggtaagac cggttggctt
      961 taccgcaaga tcccctgtat aggatctgat atactcagca gcctcttcgg gatttttgca
     1021 gagtttgtat gcaggacagc cgtcaattcc atatttctgc atcagacttc tgcaaaatcc
     1081 tttgtcagtc tcaatgcgtg ctgctgcctt tgacggacct acacatccga tacctgctgc
     1141 aaccagtgca tcagagacac ctgccgcgag cggggcttca ggacccacaa ccgcataatc
     1201 aatgtcgtgc tcttttgcaa atgcgagcac agcatcggca tctgtttcgg gatgaatcag
     1261 tacttcgcgg cagagctttg cgattcctgg gttcttctta cccattacgc agtacagatc
     1321 tgtttttgta ttcttcgaga gtgcgagtgt gattgcgtgt tcccgtcctc ctccacccac
     1381 aacaagtatt ctcatgttca tatctgtctc ctgttttgca aataatttag cggtttaaca
     1441 actcacttgc tctgaccaga ggatgcagcg ttacgccctc tgctgcaagc agtgcttccg
     1501 ccccgctgtc gcgatcgacg acaacaacaa ctgagtcaac aatagctccc gctttgcgca
     1561 gctcatcaat tccatacttt gaggaaccac ccgatgttgt tacatcctca attaagagga
     1621 catgctttcc ggcaacatca ccgatgacca tctggctttt tccgtgatcc tttgctgcgg
     1681 cacggatgac agcacagggt tttccgcttg caagcgatac tgcaaccgcg agcggtactc
     1741 ctcccacggc aacaccggca acagcatcaa acgtgtagcg ttcggcaacc tcttttgcaa
     1801 tctcgccaag cagtgcaggc tgcatgattg ctgtctttac atcgatgtaa tatttgcttt
     1861 ttacaccgga agcaagagta aaatctccga actccacggc tttatattgg attaacagat
     1921 ctaataccgg attttccatg atctccgcaa aactcctgat gaaatattgt gtactatagg
     1981 aggtcgaaga acattaaata ttcccaggat tttctggtat cgttttctac atctcttgat
     2041 ctgtgggtgc tcttacctct gtaaaatatg gataaccgga aattccggtt accagggaac
     2101 ctgtttgata cctatcaggt atccgataat gtttgccccg cggtggagaa gcggtgtcca
     2161 gatgagaatt gcaataaaga tccagatggt catgtttgca gcaatccacg gccaggcaaa
     2221 gactgcaaga agcacgaggg atcctgcgac cagatcgtac atatccgcaa ttggccactt
     2281 ttccccgcga tcttttcctg cgcggcgttt aaagaaactt tttaccatgt ctccaaggag
     2341 cgcaccggcg gcaagggcgg ttatggttac aatggtctgt tccggcaacc agtcccagtg
     2401 aaatgtctgt gcaagaaaga tctggagaag accaaacaga attccaacaa cgattcctcc
     2461 gataagtccg cgccatgttt ttccgtcccc aagatatctg cgtccgtctt ttgcacattt
     2521 tccaaagtcc accggagtac cgccgccgaa tgggacggct cctgcatttg caacatacgc
     2581 gggaaccatt atccagaatg ctgcaacaaa cgtcgtcaca actgcaagaa ttgtctccac
     2641 gatcatcaga tcacctgatc catagtttat ttaatagaaa gcagaaagat ataataactt
     2701 acattttggc ggtctgatcc gccaataccc aaggagatag cgggtccatg aaaattatca
     2761 aagaaacaaa aggcatgtgt ccggtctgcg gcaaactgct ccccgcaaca gttactgaag
     2821 aggatggagc tatctggatt cgcagaacct gcccggacca tggagagttc cgatcccttt
     2881 actggtcgga tgcagagatg taccaccggt ttgagcagta tgaccgtgaa ggccgtggtg
     2941 tctataatcc gaacaccaat actcccggtg aatgtaccca gcgctgtggt ttatgttccc
     3001 atcacaaatc cggtaccctt cttgcaaac
//
LOCUS       DALUAJ010000108         1963 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_19675,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000108 DALUAJ010000000
VERSION     DALUAJ010000108.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19675"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            155..>1963
                     /locus_tag="O0X37_02350"
     CDS             155..>1963
                     /locus_tag="O0X37_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HJK61801.1"
                     /translation="MGTIDMVYRLGPGCEVDDIVEGQVYLGKVQGFATFGTFVLLNDR
                     VKGLLHKSNVKTEKKERDQILVQVNQIRPNGNIDLREVQLPEGSYETQLVTKKITLSR
                     LSDLKNKIGRNVTIEADVVQIKQTSGPTIFTVCDDSGVEDAAAFTEAGVRSYPEVNLG
                     DIVRIFGEATRRNNQIQVEVSDMHVLKGTEADAVRARINKALEARAEPPEVTPLIESP
                     VLDALWPEMRKLAKIVRRAVLTQQPIILRHHADADGICAAVAVETAVLQLIRDTGGDP
                     DQDNFLFRRSPSKAPFYEIEDVTRDLDMMLKDNVRFGQKLPLILLMDNGSTEEDMPSY
                     KMTEVYQLDVVVADHHHPDETIDKYLLAHVNPYHVGGDFGVTAGMLGTEIARLINPAV
                     EARILHFPSVAGVADRSEAPELDAYLALIEGKYSRDECKDMALALDYEQYWLRFNDGR
                     EIVKDILNLNNAPDRHNRLVALLVTEANAAIEDQMNTMMPHVKDKVLASGANLFLADV
                     EMFAHRFTFPPPGKSSGEVHDILCKRYENQPVVTLGLGPDFAVIRSRGVKMNIPQMVR
                     AMRDEMPGAGISGGGHLVVGSIKFVEGMRSEVIAKMI"
BASE COUNT          483 a          500 c          521 g          459 t
ORIGIN      
        1 ccgctggacc taccggcctg cacgcagtgt tgtgctgcac gcaggcgacc tgctggtagc
       61 acgcggccgg cgtgacggag aagagaaact gtatggtctc tgcggtgcag acaccgatga
      121 aatagaagat tttgaagact cggagtaaat ttacatgggt acaattgata tggtataccg
      181 gctaggcccc ggttgtgaag tggatgacat cgtagaaggc caggtatatc ttggaaaagt
      241 tcaggggttt gcaacattcg gtacgtttgt tctcttaaac gatcgcgtca aaggactgct
      301 gcataaaagc aatgtcaaga ccgagaaaaa agagcgtgat cagattcttg tacaggtaaa
      361 tcagatccgc ccgaacggaa acatcgatct gcgcgaagtg cagctgcccg aaggaagcta
      421 tgaaacgcaa ctcgtcacca aaaagatcac actatcccgt ctttcagacc tgaaaaacaa
      481 gatcggcaga aacgtcacca ttgaggctga tgtcgtgcag attaagcaga catctggacc
      541 taccattttc accgtttgtg atgactccgg tgttgaggat gctgcagcat tcaccgaagc
      601 tggtgtcaga tcttatccag aggtcaatct cggtgacatc gtccgtatct ttggagaggc
      661 aacccgcaga aacaatcaga tccaggttga ggtctccgac atgcatgtcc tcaaaggaac
      721 cgaggcagat gctgtccgtg cacgtatcaa caaagccctt gaagcccgtg cagaacctcc
      781 tgaagttacc ccgctcatcg aaagcccggt gcttgatgca ctctggccgg aaatgcgtaa
      841 gcttgcaaag atcgtccgcc gtgcggtact gacccagcag ccgatcattc tccgccacca
      901 tgcggatgct gacggtatct gtgccgctgt tgctgttgag acggcagttc ttcagttaat
      961 ccgtgacact ggcggcgatc cggatcagga caacttcctt ttccgcaggt ccccctccaa
     1021 agccccattc tatgagattg aggatgtaac ccgcgatctt gacatgatgt taaaggataa
     1081 cgtccggttt ggccaaaagt tgccgttgat tcttctgatg gacaacggtt caaccgaaga
     1141 ggatatgccc tcctacaaga tgaccgaggt gtatcagctt gatgtcgttg tcgccgatca
     1201 ccaccatccg gacgaaacca ttgacaaata tctgcttgcc catgtcaacc cctatcatgt
     1261 tggcggtgac tttggtgtca ccgcaggaat gcttggaact gaaattgccc gcctgatcaa
     1321 tccggctgtt gaagcacgga tccttcactt cccctcggtt gccggtgttg cagaccgcag
     1381 tgaggctccc gagcttgatg catatctggc actcattgaa ggtaaataca gccgggatga
     1441 gtgtaaggat atggcacttg cactggacta cgagcagtac tggctcaggt tcaatgacgg
     1501 ccgtgagatt gtaaaagaca tcttaaacct caacaatgca ccagaccgcc acaaccggct
     1561 ggttgcgctt cttgtaaccg aagcaaatgc agcaattgag gatcagatga acacgatgat
     1621 gccgcatgtc aaagacaagg ttctggcatc tggggccaac ctcttccttg ccgatgttga
     1681 gatgtttgcc caccgcttca ccttcccgcc gccgggcaaa tcctccggtg aggtccatga
     1741 tattctctgc aagcgttatg agaatcagcc ggtcgtaacc cttggccttg gtccggactt
     1801 tgcggttatt cgttcccgcg gtgtgaagat gaacatcccc cagatggttc gtgccatgcg
     1861 cgatgagatg ccgggagcag gaatttcagg cggcggtcat ctggttgttg gtagtatcaa
     1921 gtttgttgag ggaatgcggt ctgaggtcat tgcaaagatg atc
//
LOCUS       DALUAJ010000109         3821 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_19707,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000109 DALUAJ010000000
VERSION     DALUAJ010000109.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19707"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="O0X37_02355"
     CDS             <1..571
                     /locus_tag="O0X37_02355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PGF-CTERM sorting domain-containing protein"
                     /protein_id="HJK61802.1"
                     /translation="LQSSNAADALTKMIDNPNIDDVYTKLTFTVVPAMITINNPGDQG
                     VGTKFTISGTTNLAVGDQIIVEVQSSSFTAVDKSSTSSTDGVSVTTKVVAGEGTDNVW
                     SVEVDTTNWKLDEYTIRAEGIEVDVSATTNFNLVEKTVTPTTTATATGTATATATATA
                     TATPATPGFGAFIALAGLGAVALLVLRRN"
     gene            complement(959..1843)
                     /gene="argB"
                     /locus_tag="O0X37_02360"
     CDS             complement(959..1843)
                     /gene="argB"
                     /locus_tag="O0X37_02360"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HJK61803.1"
                     /translation="MNRQSVLMEALPYIRKFHGKTIVIKLGGHAMVDPKIMNTVIEDA
                     VLLHYVGMRVVLVHGGGPEITEKMKALGKEPKFIGGLRVTDAETLEIAQMVLAGKISS
                     AIVSLIAKNGAHGVGVSGNDGGLVIAEKMPLKKIMVGDHEEEVDLGFVGEIREINSKL
                     LETLLDAGYIPVVSPLAIDRKGNDLNINADTMAGEIAVALKAFKLISLTDVDGVMDKE
                     RTQVFHRLTLKNVDALMADGTISGGMIPKLEASVNAVRHGVEGAHVVNGNAEHNLLLE
                     LFTDDGVGTMITASMISF"
     gene            complement(1840..2991)
                     /gene="argJ"
                     /locus_tag="O0X37_02365"
     CDS             complement(1840..2991)
                     /gene="argJ"
                     /locus_tag="O0X37_02365"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HJK61804.1"
                     /translation="MQSICAVEGVTAWGVKEGKYGLALIKASGTGAAVFTSNKVRAPV
                     VNLMAERTKRGKLAGIIVNSGCANAYTGKRGYEDAVTMASIGAEALGISEKETGVAST
                     GVIGRYLDLDLIRRQSADVAGKLAHSADAEKAAAAAIMTTDTVQKHALVQREGFTVAG
                     ICKGSGMIAPNMGTMLSFVYTDAEVSAEYLHDALKSAVRRSLNRVVVDGDESTHDSLF
                     CTATGEAGRVPKKEFTAALEECCVSLAKQIAADGEGATKLLEVRVKGARREKDAEKIA
                     RAVITSPLVKSAVYGEDPNWGRVVCAAGYSGVEFDIDELSLSIGEGESETSLVRKGEI
                     TADLTKAKAAMTGKHVVFTITLESGKKEATAWGCDLTEQYVEINGKYTT"
     gene            complement(3038..>3821)
                     /gene="argC"
                     /locus_tag="O0X37_02370"
     CDS             complement(3038..>3821)
                     /gene="argC"
                     /locus_tag="O0X37_02370"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HJK61805.1"
                     /translation="LAVPHTAAMQYALQLRERGIKTVDLSADYRLPQEIYEKTYGVKH
                     TAYFKAPYGIPELHRNEVRGADFVANPGCFPTGATLAAAPVADIAATIIYDSKSGVSG
                     AGDSVSETTHYPNVDENIAPYKITAHRHLPEMKQEAAFLGSSAKIYFTPHLLPAIRGI
                     ITTAHILFDEPVSLEEVKRRYETFYKDEPFVRLQTAKLGGVRGSNFCDINFELEADGT
                     RLVAVSAIDNLVKGAAGQAIQNMNIMCGYAETDGLRMPGMFP"
BASE COUNT          954 a         1137 c          813 g          917 t
ORIGIN      
        1 acttcagagt tcgaatgcag ctgatgcgct gacgaagatg atcgacaacc cgaacatcga
       61 tgatgtttac accaagctga ccttcaccgt tgttccggca atgatcacaa tcaacaaccc
      121 aggagaccag ggtgttggca ccaagttcac tatctccggt acgaccaacc tcgctgttgg
      181 cgatcagatc atcgttgaag tacagtcctc atccttcact gcagttgata agtcctccac
      241 ctcatccacc gatggtgtct ctgtaaccac caaggttgtt gcaggcgagg gtactgacaa
      301 cgtctggtct gttgaggttg acacgaccaa ctggaagctt gatgagtaca ccatcagagc
      361 cgaaggtatt gaggttgatg tatcagctac gaccaacttc aaccttgttg agaagactgt
      421 aaccccgacc acaaccgcaa ctgcaaccgg aaccgcaact gcaactgcaa ccgcaactgc
      481 aaccgcaacc ccggcaactc cgggcttcgg cgcattcatt gcactcgcag gacttggtgc
      541 agtcgcactg ctcgtcctcc gccgcaacta agcagcgtga aataaaagag agatgaacca
      601 tcctcaggag aactcctgag agatacaaaa tctctttttt gagagaaatg tgaacgatct
      661 tttttcaaga atttttcaaa cgaactggca atacaaaatc cagtacaaaa aaagaaaaaa
      721 attatttcgc cagcaagtga tttgccacct taataaacaa acactcgccg gtccccagct
      781 cataaaacgt atctgccttc ttatcgatct tcgcatcagc tcgccagaac ttacactctt
      841 tacccataca tctctcctgc gtcatcggac aaataaatcc cacaaaatca cctttttctg
      901 agaatatcga atcacaaaaa taaatcactt tcgttttgca aaacaaaaaa aagaaaaatt
      961 agaagctgat catcgacgca gtgatcattg ttcctacacc atcatccgtg aacagctcaa
     1021 gaagcagatt gtgctccgca tttccgttca ccacatgggc accctcaacg ccatgccgga
     1081 ccgcattcac actcgcctca agtttcggaa tcataccgcc ggagatagta ccgtctgcca
     1141 tcagagcatc cacattcttc agagtcagcc ggtggaacac ctgcgtccgt tccttatcca
     1201 ttacaccatc cacgtccgtc agagagatca gcttaaacgc cttcagtgcc accgcaatct
     1261 caccggccat cgtatccgca ttaatattca gatcattacc cttccgatca attgccagcg
     1321 gagacacaac cggaatgtat cccgcatcca gcaacgtctc cagcagcttt gaattgatct
     1381 cgcgaatctc tcccacgaac ccgagatcca cctcctcctc atgatcaccg accattatct
     1441 tcttcagagg catcttctcc gcgatcacaa gaccgccgtc atttccggaa accccaaccc
     1501 cgtgagcacc gttcttcgca atcaggctca caattgcact actgatcttt ccggcaagca
     1561 ccatctgggc aatctccaga gtctccgcat ccgtcacccg cagaccgccg atgaacttcg
     1621 gctccttacc cagagccttc atcttctccg taatctccgg accgcctccg tgcaccagca
     1681 caacacgcat ccccacataa tggagaagca ccgcatcctc aatcacggta ttcataatct
     1741 tcggatcaac catcgcatgg ccgccgagct taattacaat cgttttgccg tggaacttcc
     1801 ggatgtacgg aagtgcctcc attaacacac tctgtcgatt catgttgtat acttcccgtt
     1861 gatttccaca tattgttccg tcagatcgca accccacgcc gttgcctcct tcttcccgga
     1921 ctcaagcgta atcgtaaaca ccacatgttt accggtcatt gcagcctttg ccttcgtgag
     1981 atccgcggta atctctccct ttcgaacaag cgatgtctcg ctctcgccct caccaatcga
     2041 aagcgacagt tcatcaatat caaactccac accagagtac ccggcggcac agacaacccg
     2101 gccccagttc ggatcctcgc cgtagaccgc actcttcacc agcggtgacg taatcactgc
     2161 cctggcaatt ttttccgcat ctttctcacg gcgtgctccc ttcacccgga cctcaagcag
     2221 ctttgttgcg ccttcaccgt ctgcggcaat ctgctttgca agcgaaacac agcactcctc
     2281 aagtgctgcg gtaaactctt tctttggaac acggcctgcc tcgccggttg cagtacaaaa
     2341 aagcgagtcg tgtgtactct catccccgtc caccaccacg cgattcagac tccgccgtac
     2401 cgcactcttc agcgcatcat gaagatactc agcagagacc tccgcatcgg tgtacacaaa
     2461 cgaaagcatt gtacccatgt tcggtgcgat cataccggat cccttacaga taccggcaac
     2521 cgtgaaccct tcacgctgca ctaaagcatg cttttgtacc gtatcagtcg tcatgattgc
     2581 agcagctgcg gctttctccg catcagcaga gtgagcaagt tttcctgcaa catctgcact
     2641 ctgacggcga atgagatcca gatcaaggta tctgccgata actccagtac tggcaacacc
     2701 tgtctccttc tcagagatgc caagtgcctc agcaccaatc gatgccatgg taacggcatc
     2761 ctcgtacccg cgttttccgg tgtacgcgtt ggcacatccg ctgttcacaa taatacctgc
     2821 aagctttccg cgtttggtac gttccgccat gaggttgaca accggagcac ggactttatt
     2881 gctggtaaaa acagctgcac cggttccgga tgctttgata agtgcaaggc cgtattttcc
     2941 ttcctttacg ccccacgcgg tgacgccttc aacagcacag atactctgca tatgaattcc
     3001 tgtctattat atattgttct gggtatcttg aatgttctca cgggaacatt ccgggcattc
     3061 tcagaccgtc tgtctctgca tatccgcaca taatattcat gttctggatg gcctgacctg
     3121 ccgcaccttt gacaaggttg tcaattgccg agaccgcaac aagccgcgta ccgtcagcct
     3181 caagctcgaa gttgatatca cagaagttgg atcctcgaac acctcccagt tttgcagtct
     3241 ggagacggac aaaaggctca tccttgtaga aggtctcata ccgtcttttt acctcctcca
     3301 gactaaccgg ctcgtcaaag aggatatggg ccgtggtaat aatgcctcgg attgccggaa
     3361 gcagatgggg agtgaagtag atctttgcag aagaacccag gaaggcagct tcctgtttca
     3421 tctcgggaag atgacggtgt gctgtaattt tgtacggggc aatgttctca tcgacgttag
     3481 ggtagtgggt tgtctctgaa actgaatcgc ctgccccgga gactccgctc tttgagtcgt
     3541 agataattgt tgcagcaatg tcagctaccg gagctgcggc gagggttgcg ccggttggaa
     3601 aacatcccgg atttgcaaca aagtcggcac cgcgtacttc gttgcggtga agctcgggaa
     3661 ttccgtacgg agctttaaag taagcggtat gcttgacacc gtaggtcttt tcataaatct
     3721 cctgcgggag ccggtaatcc gcggagagat caacggtttt gatcccgcgt tcccgaagct
     3781 gaagagcata ctgcattgct gcggtgtggg gaactgcaag g
//
LOCUS       DALUAJ010000110         1691 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_19940,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000110 DALUAJ010000000
VERSION     DALUAJ010000110.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19940"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(382..>1691)
                     /locus_tag="O0X37_02375"
     CDS             complement(382..>1691)
                     /locus_tag="O0X37_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proline symporter"
                     /protein_id="HJK61806.1"
                     /translation="AIGTYLNWKFIAKRLRIYTHKAKDSLTIPEFITNRFQEKKGFIT
                     AIASIFILIFFIVYTSAQFKAGGVLFSSLMTIYGVDLTSIGITDASSLYIAGVLICAL
                     IVVCYTFTGGFKAVCITDTIQGMLMLFALILVPITVCIILFGEGASFSSIDPQMFSLF
                     HEYNAASGIFEFVSVFTIVSGLAWGFGYFGQPHILPRFMAIENPEDVPKARMAAMIWV
                     VLSLAAAIFVGLVGQLLFPNPAVVGDSEQIFIQMTGSVFGAVPFIAGIIYCGILGAIM
                     STASSQLLVASSAISQDLYKNLINKEAPDKRLLWISRVTVLIVSVVAIALSLDQASSV
                     FNIVSNAWAGFGCTFGTVILLGLFWKRMNWQGALAGIIVGGIVSLGWGTYLTAVTNLY
                     EMVPGVIASLLAIYLVSRFTAEPEKEVTDMYDEYVAAVGDRST"
BASE COUNT          466 a          408 c          417 g          400 t
ORIGIN      
        1 attacattgt aatatttcga tggtcgtgca tcaccggtac agtgaaaaag tacatccggc
       61 acctgaagga agtatcattt ttcctgtctc ggtgcggcac gtatgttgtt ggcatttcga
      121 cccatcggat attactctaa aaccatcgcg gtcataatga tacccggtat cgaggccgcg
      181 gagaacttca gccttgtacc taactccccg acctggtgca gaaatgcagt ggccaggttg
      241 gctgaaggac acgttttgga tctgttttac atgttttttg cagatgtgta caccgtattc
      301 gcattgccac atctttttca ctaatgggtg tctttgtcaa aagcgattga agagataatc
      361 tgaaaaaaga aacgggagtt tttaggtact ccggtcacca actgctgcta cgtactcatc
      421 gtacatgtcg gtgacttcct tctccggctc ggcggtgaac cgcgagacaa ggtagattgc
      481 aagcagactt gcaattacac ccggcaccat ctcatacaga ttcgtgaccg cggtcaggta
      541 agtaccccag ccaagagaga cgatacctcc cacgatgatt ccggcaagtg caccctgcca
      601 gttcatccgt ttccagaaga gacctaaaag gatcactgta ccgaacgtac aaccgaatcc
      661 tgcccatgcg tttgatacaa tattgaacac tgagcttgcc tgatccagtg agagggcaat
      721 tgcaactact gagacgatca gaaccgttac acgggagatc cagagcagcc gcttgtcagg
      781 agcttctttg ttgatcaggt tcttgtagag atcctgtgag attgccgatg atgccaccag
      841 aagctgagat gatgcggtac tcatgatcgc accgaggata ccacagtaga tgatacctgc
      901 gatgaacgga actgcgccga acacacttcc ggtcatctga ataaagatct gttcagagtc
      961 accgacaact gctgggttcg ggaacagaag ctgtcccaca agacctacaa agattgcagc
     1021 ggcaagagag agaaccaccc agatcattgc tgccatacgt gccttgggga catcttcggg
     1081 gttctcgatt gccatgaacc ttgggagaat gtgcggctga ccgaagtagc cgaatcccca
     1141 ggcaaggccg gaaacgatcg taaacactga tacgaactca aagataccgc ttgctgcatt
     1201 gtactcgtgg aacagtgaga acatctgcgg atcgattgag gagaagcttg ctccctcacc
     1261 gaacagaatg atacaaacgg tgatcggtac aagaatcaga gcaaacagca tcagcattcc
     1321 ctgaatggtg tcggtaatac agaccgcctt gaagcctccg gtaaaggtgt agcagaccac
     1381 aatcagtgca cagatcaata cccctgcaat gtagagcgag gatgcatccg taattccgat
     1441 ggaggttaag tcaacgccat aaatcgtcat cagagagctg aacagaacac ctcctgcctt
     1501 gaactgtgct gaggtataga cgatgaagaa tatcagaata aagatagatg caattgcggt
     1561 gatgaaccct ttcttttcct ggaaacggtt cgtgataaac tcgggaatcg ttagtgagtc
     1621 ctttgctttg tgcgtgtaaa ttctcaggcg ctttgcaatg aacttccagt tcagataggt
     1681 tccgatggca a
//
LOCUS       DALUAJ010000111         6568 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_20482,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000111 DALUAJ010000000
VERSION     DALUAJ010000111.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20482"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(116..1621)
                     /gene="lysS"
                     /locus_tag="O0X37_02380"
     CDS             complement(116..1621)
                     /gene="lysS"
                     /locus_tag="O0X37_02380"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0017101 - aminoacyl-tRNA synthetase
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HJK61807.1"
                     /translation="MNEKIHWADAKASRVPADRHQLIATGITPSGPIHLGNMREVMTG
                     DMIYKALKSRGVDAELVYVADDFDPLRKVYPFLPDSYQKYIGWPVSDIPCPCGEHKSY
                     AEHFLEPFLSAIEELDIHPRIIRTSESYRSGMFTEQIKASLEHAEEIKGILERISGRK
                     LEEGWTPYYPICEKCGIISRATILSHDPENHTVTYRCSCGHEGVSDYSKGEGKLVWRV
                     DWPMRWSAYGVTIEPFGKDHASPGGSYDTGKCITKEIYGYEAPIPVTYEWISLKGRGE
                     MHSSKGVVLTIRDMLDIVPPEVLRYMIAKTKPDKTIAFDPGMGLLRLIDEYDRAAASG
                     DSREYELSVIASPQTTIPFRHMVTVVQIAQNDEGIFDILKRSGYEIVDRNAVLDQANR
                     ARMWLERYAPEDVKFAIQPILPEIAAAEPANVKAAVAAYATAVAADEWKADVLHNAVY
                     DAAASAGITGKELFTAIYRAFLGADRGPRLGWFLEAIGREATLARLNEMTA"
     gene            complement(1784..2896)
                     /gene="cofH"
                     /locus_tag="O0X37_02385"
     CDS             complement(1784..2896)
                     /gene="cofH"
                     /locus_tag="O0X37_02385"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HJK61808.1"
                     /translation="MTAPTLNSAELARILDDALAGSRMSETEAAALFRLTGRDIWKVA
                     AAADERREEVVGDTVTYVRNMNLHMTNICKNRCGLCAFGRKPTDPDAFCFTDEEFREH
                     ARDAAKKEVTEVCYLAGVHPGFTIENYETMIRTLCEEIPGVHVHGCSPDEIAFAAQRS
                     GLTTKETLIRLKEAGLGSVQGTAAEILVDSVRKVICEKKIPTEEWVRIIREAHEVGFK
                     ATSTIMYGSVETPEERAHHLSILRDLQDETQVFTELVPLAFLHHNTPLEKDGTVTYGS
                     TGREDILLTAVSRLFLDNMKNIQVPWSKIGQKMTQLGLMSGGNDVGGTMFVDSLSRTA
                     GAGEEADFFDPKDMQYLCDDMGRKLARRDTLYNILE"
     gene            complement(2893..3615)
                     /locus_tag="O0X37_02390"
     CDS             complement(2893..3615)
                     /locus_tag="O0X37_02390"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HJK61809.1"
                     /translation="MSKSPKKAESNADADDVQKSSLMKILKSDDPKISLIRDILSVVI
                     VVAIIGGVLFGVSGTWPAIVAVESGSMVPNMNVGDLIFVVEENRFGPLLTAEEAEAAG
                     VTSFNGYGDVIIYRPNGNTQVTPIIHRALTEINASTAAEMGFTGEAAHAGYITKGDHN
                     EIIDQYGNFSNNIGRMQPVKEEWIVGKALFAIPLLGYLPLHIWEFAVVVVILLVIWEL
                     YSRKKEKDREVTKKPQKKGGRK"
     gene            3809..4306
                     /locus_tag="O0X37_02395"
     CDS             3809..4306
                     /locus_tag="O0X37_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HJK61810.1"
                     /translation="MVELTIKKRHAIKKSQLASLMQKLTDTIGSDAALYNASMIEIAE
                     TASKFNIYIIDKKPLIMEQDTWAFPTLRGAVLRPFSGHRIVVDMGAVPYMINGADVMR
                     PGIVSVTDDVVAGKPALVVDEIHDKPLAVVIPLYDAAGIIALEKGKAAKNMHYIGDEL
                     WNLEL"
     gene            4358..4732
                     /gene="sepF"
                     /locus_tag="O0X37_02400"
     CDS             4358..4732
                     /gene="sepF"
                     /locus_tag="O0X37_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HJK61811.1"
                     /translation="MGFLDGVFGSKKDTLTEDEYMKLDLASYEGVAGAEEPAVMYVKV
                     ASIADIKDCPRVKDEVYNNNIVIVDITKLKLDKIMFDRVMGDLRAVSKDVNGDIIGLG
                     DQRYVIITPTGVRISREKIGGM"
     gene            4738..5292
                     /locus_tag="O0X37_02405"
     CDS             4738..5292
                     /locus_tag="O0X37_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZPR1 zinc finger domain-containing protein"
                     /protein_id="HJK61812.1"
                     /translation="MRQVVPGPCPDCGKQIEYIYDTENIPYFSDILLLSGVCPDCGFR
                     VTDTMILNDHEPCRWEMNVETPEDLDARVIRSMQGEVDIPEFGINIRPGPACSGFVSN
                     IEGVLLRAEDAIRRALPSCEGDEITTAHELLEKIDQARASQFPFTVIISDPSGNSGIV
                     SSKAVKTKLDVEPQPDGCTIHPLA"
     gene            5308..>6568
                     /locus_tag="O0X37_02410"
     CDS             5308..>6568
                     /locus_tag="O0X37_02410"
                     /inference="COORDINATES: protein motif:HMM:NF020804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJK61813.1"
                     /translation="MDSGAKRHLLTDAEETRLRKLIENRSSDSRLDSLTLAYIEKISG
                     KKWKDESVLEKIRAAITVQKDSYWKEGERRVVQYKGGYSVLGYMAYQMPGYVAEFTEF
                     FVRLLREGLIRDHVRILDVGAGPGTVAVAVARVLEMCDGITAEVVSLERSEVFREAYM
                     AVVPAFVKDAGDHVTAPRPIAADITETMPEGEFDLIVCSNMINELAVYGGEKTDLMMQ
                     LSTHLVADGNLILLEPADLANATMLRDLSRDLKRRGLTLYAPCNDIRGVHCSVSPCWT
                     FETYADIRPTKLMFALGGDTEKFRFVNTDVKFSYAILRKDGHRRCGYRVPAAAKRARL
                     AQLKKHVGKRIHVTVSVMSADIGDAKNYLYLVCDGTGEVPVYVALPAHHRNPEHEALL
                     SAPYGSVVAIDSVLVRFNPKQNAYNLLM"
BASE COUNT         1448 a         1653 c         1716 g         1751 t
ORIGIN      
        1 cagcagtcgc gatcacacct tccggtaatg aagaggacaa gctttgctcc ttgatagcag
       61 agcttgcatc cctcactgag atttttcggg agtttgtctt ttgcttttgc cattgttatg
      121 ctgtcatttc gttcagccgt gcaagtgttg cttcacggcc gattgcttcc aggaaccagc
      181 cgagacgcgg tccgcggtct gcaccaagga aggcgcggta gatggcggta aacagttcct
      241 ttccggtgat acctgcggat gctgcagcat cgtagactgc attgtgcaag acatcggcct
      301 tccattcatc cgcagcgacc gcagttgcat atgcagcaac agctgcctta acgtttgccg
      361 gttctgctgc tgcaatctcc ggaaggatcg gctggattgc gaacttcaca tcctccgggg
      421 cgtagcgctc aagccacatt cttgccctgt tcgcctgatc cagtactgca ttcctgtcca
      481 caatctcgta gccgctgcgt ttcaggatat cgaagatacc ctcatcgttc tgggcaatct
      541 ggactaccgt gaccatgtga cggaatggaa tcgtggtctg cggggaggcg ataaccgaga
      601 gttcgtactc gcgtgaatca ccggatgctg cggcgcgatc gtactcatcg attaaccgga
      661 gaagacccat gccgggatca aacgcgatgg tcttgtccgg ttttgtcttt gcgatcatgt
      721 aacggagcac ctccggcggg acgatgtcaa gcatatcgcg gatggtcaga acaacacctt
      781 tggaggagtg catctcaccg cggcccttca ggctgatcca ttcataggtt accgggatcg
      841 gtgcttcata tccgtagatc tctttagtga tgcacttgcc ggtatcgtag gatcctccgg
      901 gagatgcgtg gtctttgccg aacggctcaa tggttacgcc gtatgccgac cagcgcatcg
      961 gccagtccac acgccagaca agttttccct cgcctttgga gtagtcagag acaccttcgt
     1021 gtccgcatga acaccggtag gtcacagtgt ggttttccgg atcatgggaa agaatggttg
     1081 ctcgggaaat gattccgcac ttctcacaga tcggatagta cggggtccac ccctcttcga
     1141 gtttgcggcc ggagattctc tcaagaatcc ctttgatctc ttctgcatgt tcgagggatg
     1201 ccttgatctg ttcggtgaac atgccggacc gataggactc acttgtccgg ataatgcggg
     1261 ggtgaatgtc aagctcctcg atcgcggaga ggaatggttc aaggaagtgc tcggcgtagc
     1321 ttttgtgttc tccgcagggg cagggaatat ctgagaccgg ccacccgatg tatttctggt
     1381 atgagtcggg gaggaacgga tagactttac ggaggggatc gaaatcgtct gcaacataga
     1441 cgagttctgc gtcaacacca cggcttttca gtgccttgta gatcatgtcg ccggtcatga
     1501 cttcacgcat gtttccaagg tgaatcggtc cggacggggt gattcctgtg gcaatgagct
     1561 ggtgtctgtc ggccgggacg cgggaggctt ttgcgtcggc ccagtggatt ttttcgttca
     1621 cgcttttgta ctcctctgaa tgtataatta agatctatgt gttttccagt gagatatatc
     1681 tcatgggcat attctcaggg attttgcaat aattgggtcc gcttttcctg agtgagataa
     1741 tgcaatgtca gtacgcgccg gtactcgtcg gcaaaaaaaa gagttattcc aagatgttgt
     1801 acagcgtatc gcggcgtgcg agttttcgac ccatatcatc gcaaaggtac tgcatatctt
     1861 ttggatcgaa gaagtctgct tcttctccgg cacctgcggt ccgtgacagt gagtccacaa
     1921 acattgttcc gcctacatcg tttccgccgg acatcaggcc aagctgtgtc attttctgcc
     1981 cgatcttcga ccacggcacc tgaatgtttt tcatgttatc caagaacagc cgggagactg
     2041 ctgtcagaag aatgtcttcc cggccggttg atccgtacgt gaccgttccg tccttttcaa
     2101 gcggtgtgtt gtgatgcaga aatgcgagcg gcacaagctc tgtgaacacc tgtgtttcgt
     2161 cctgaaggtc gcggagtatt gagaggtgat gagcacgctc ctctggagtc tccaccgatc
     2221 cgtacataat tgtcgaggtc gccttgaacc cgacctcgtg tgcctcgcgg atgatccgca
     2281 cccactcctc ggtcgggatc tttttttcgc agattacttt acggaccgag tccaccagaa
     2341 tctccgcggc ggttccctgc accgatccaa gtcctgcttc ctttaaccgt atcagtgtct
     2401 cttttgttgt gagaccgctc ctttgtgctg caaatgcaat ctcatccggg ctgcatccgt
     2461 gtacatgaac cccaggaatc tcctcgcaca gtgtacggat catcgtctca tagttttcaa
     2521 tcgtgaatcc tggatgaacg cccgccagat aacagacctc ggtgacctcc ttctttgctg
     2581 cgtcgcgtgc atgttcccgg aactcctcgt cggtaaagca gaaagcatcc gggtcggtgg
     2641 gctttcgtcc aaatgcacac agtccgcagc ggttcttgca gatgttggtc atgtgcaggt
     2701 tcatgttccg gacataggta acggtatctc ccacgacctc ttcgcggcgt tcatcagctg
     2761 cggctgcaac tttccagatg tcacggccgg tcagccggaa cagggctgcc gcctctgttt
     2821 cactcatgcg tgatccagca agtgcatcgt caaggattct tgccagctct gcactgttca
     2881 gtgttggtgc agtcattttc gcccaccttt tttctgaggt ttcttggtta cttctctatc
     2941 tttttctttc ttgcggctgt acaactccca gatcaccagg aggattacga caacaaccgc
     3001 aaactcccag atgtgaagcg ggaggtaacc caacaggggt attgcaaaca gtgcctttcc
     3061 gacgatccac tcctctttta ccggctgcat tctgccgatg ttgttgctga agttgccgta
     3121 ctgatcaatt atttcgttgt gatcaccttt ggtaatgtat cctgcatggg cagcttctcc
     3181 ggtgaatccc atctctgctg cggttgatgc attgatctct gtaagtgccc ggtgaatgat
     3241 cggcgtcacc tgtgtattcc cgttcggccg gtagatgatc acatcgccgt atccgttaaa
     3301 agatgttact ccggcggctt cagcctcttc tgcagtcagg agaggtccga accggttctc
     3361 ttcgaccaca aagatcagat ctccgacatt catgttcggc accatactcc ctgactcaac
     3421 tgcaacgatc gcaggccagg ttcccgacac accgaacagc acaccgccga ttatggcaac
     3481 aacgatgacc accgagagaa tatcacggat aagactgatc tttggatcat ccgatttcag
     3541 aatcttcata agagaggatt tttggacatc gtccgcgtct gcattacttt ctgctttttt
     3601 gggcgacttc gacataatgc tgtatctaac taagtcctca ctggtataga agtttcccgt
     3661 aataccctgt tgttcgtcaa tcctcagtac tggatcatac ctgtccgtct tctgtctttg
     3721 ggatctgttt tttctgtttt tctcaaggga ccttttgcct ggcaaactga accattatag
     3781 tttcctgtaa ccaacggagt atgcacaaat ggtagaactc acgattaaaa agcgtcacgc
     3841 aatcaaaaaa agtcagctgg catctctcat gcagaaactt accgatacca ttggttctga
     3901 tgctgcactt tacaatgcct cgatgattga gattgctgag actgcctcaa agttcaacat
     3961 ctatatcatc gacaaaaaac cgcttatcat ggaacaggac acttgggcat tcccgacact
     4021 tcggggtgct gtccttcgtc ccttctccgg tcataggatc gttgtggata tgggcgcagt
     4081 tccctacatg atcaacggcg cagatgtaat gcggcccgga attgtctcag tgactgatga
     4141 tgttgtcgca ggaaaacctg cccttgtcgt ggacgaaatc cacgataaac cgcttgccgt
     4201 tgttattcct ctgtatgatg ctgccggcat catcgcgctg gaaaaaggaa aggccgcaaa
     4261 gaatatgcac tacatcggtg acgagctctg gaatcttgaa ctctgatctg tgatcatacc
     4321 aaagcattaa ataaccaaca aattaactat tttttatatg ggatttctcg acggagtttt
     4381 cggatcaaaa aaggacaccc ttactgaaga tgaatacatg aaacttgacc ttgcctccta
     4441 cgaaggagtt gcaggtgccg aggaaccggc ggtcatgtat gtgaaggtcg caagtattgc
     4501 cgacattaag gactgcccgc gtgttaagga tgaagtctat aataacaata ttgtgatcgt
     4561 tgatatcacc aaactcaagc tcgacaaaat catgtttgac cgcgtgatgg gcgatctccg
     4621 ggctgtttcc aaagatgtga atggtgatat cattggtctt ggagaccagc ggtacgttat
     4681 catcacaccg accggtgtcc gcatctcccg tgagaagatt gggggcatgt aaccttaatg
     4741 cgtcaggttg ttccgggccc ctgcccagac tgcggcaaac agatcgagta tatttacgat
     4801 acggaaaata tcccctattt ttccgacatt cttctgctga gcggtgtctg tcctgattgt
     4861 ggttttcgtg tgacggacac gatgatctta aacgatcatg agccctgccg ctgggaaatg
     4921 aacgtggaaa cacccgaaga tctggatgcc cgcgttatcc gcagcatgca gggagaggtc
     4981 gatattccag agtttgggat aaatatccgt ccgggtcctg catgcagtgg ttttgtgtcc
     5041 aacattgaag gggtgctgct gcgtgctgag gatgcaatcc gccgtgctct tccctcttgt
     5101 gagggtgatg agattaccac tgcacatgaa ctgctggaaa aaattgatca ggctcgtgcc
     5161 tcacagttcc cctttaccgt gatcatttca gatccctcgg ggaactcggg cattgtctcg
     5221 tcgaaagcgg tgaaaacaaa acttgatgtg gagccgcagc cggacggatg tacgattcat
     5281 cctctggcat aaactgtttt tgaatttatg gactctggag caaaacgcca tctgctgacg
     5341 gatgccgaag agacgcgtct gcgcaaactg atcgaaaacc gatcatctga ttcccgtctt
     5401 gattcgctga ccctcgctta catcgaaaaa atttcgggta agaaatggaa agacgagagt
     5461 gttcttgaaa agattcgtgc ggcaattaca gtccagaagg actcctactg gaaggaaggt
     5521 gaacgccgcg tggtgcagta caagggtggc tacagtgtgc tgggttacat ggcataccag
     5581 atgcctgggt atgttgcgga gttcacggag ttttttgtcc ggcttctccg tgagggactg
     5641 atccgggatc atgtccgtat tctggatgtg ggcgctggtc ccggcactgt tgcggttgcg
     5701 gttgcccgcg ttctggagat gtgtgacggt atcaccgcag aggttgtttc gctggaacgc
     5761 tcggaggtgt tccgggaggc gtacatggcg gttgtcccgg cgtttgtaaa agatgcaggt
     5821 gatcatgtta cagctccgcg gccgatcgct gctgatatta ctgagacaat gccggaggga
     5881 gagttcgatc tgatcgtctg ctcgaatatg ataaatgagc tggcggttta tgggggtgaa
     5941 aaaacagatc tgatgatgca gctttcaacg catctggtcg cggacggaaa cctgattctg
     6001 ctcgaacctg cggatcttgc aaacgcaacc atgctgcggg atctttcccg tgatctgaaa
     6061 cgccgcggtc ttacactgta tgcgccgtgt aatgacatac ggggagtaca ttgcagtgtc
     6121 tctccctgtt ggacgtttga gacatacgca gacattcggc cgacaaaact gatgtttgca
     6181 ttaggcggtg atacggaaaa attccggttt gtgaacacgg atgtgaagtt ttcatatgct
     6241 attcttcgca aggacggtca ccggcgctgt ggataccgtg ttcctgcggc ggcaaaacgt
     6301 gcaaggcttg cacagctgaa aaagcacgtc ggtaaacgca ttcatgttac cgtgtcggtg
     6361 atgtccgccg atattggcga tgcaaagaac tatctgtatc tggtctgcga cggaactggt
     6421 gaagttcctg tgtatgttgc ccttcctgcg catcatcgga atccggagca tgaggcactg
     6481 ttgtccgctc cctatggttc ggttgttgca attgactcgg ttctggttcg gttcaatcca
     6541 aaacaaaacg cctataatct gctgatgg
//
LOCUS       DALUAJ010000112         6366 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_20835,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000112 DALUAJ010000000
VERSION     DALUAJ010000112.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6366)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6366)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6366
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20835"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="O0X37_02415"
     CDS             <1..283
                     /locus_tag="O0X37_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK61814.1"
                     /translation="DGDDGWTLLDVNDDVAEKFIEALKHPGITVKIQRDAVSHNLTEC
                     VDCGLCISICQKKVFSFDDSWRLQVNSERCVLCGRCVMYCPQRALSLQK"
     gene            280..990
                     /locus_tag="O0X37_02420"
     CDS             280..990
                     /locus_tag="O0X37_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HJK61815.1"
                     /translation="MIREHFEFRQTITTILADDPAHIAAAKAGMLSGRADLESYLRTD
                     PFFGTSYSPVPAEPDAPLIVHRMADAAEEACVGPMAAVAAAIAWAGVESMVVSGASFG
                     LIDNGGDIVFVAADRSVRVGLYAGAAPVSGKYAFVLPPAPDIRSVCTSSATVGPSVSF
                     GVADAVVVFSHNPAKADAWATGLCNALRPTDEGIVLPKKAAIDGVYAVIGEWTAAWGT
                     VPEIVPAAVRYDMITKGE"
     gene            complement(993..1406)
                     /locus_tag="O0X37_02425"
     CDS             complement(993..1406)
                     /locus_tag="O0X37_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61816.1"
                     /translation="MVQPCSYAELTLVADDLYNRCNDDDAELARLMHDLDYDTAVQLC
                     TSDLTNAAQAYMYAFNEIPDDDVYDILLLEPSSQLLHGIRIQQVELAELIMGYDKSKK
                     LFFIGVWDGERIVAAFTGDGAYDRAVAFAREHCSV"
     gene            complement(1484..2251)
                     /locus_tag="O0X37_02430"
     CDS             complement(1484..2251)
                     /locus_tag="O0X37_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61817.1"
                     /translation="MTDIAMELTVVVAFTSLVVSIISSIYGFTQNRKLQEFEHRTRED
                     TKEDLVKLDTALKSIIIKSSYRNDMRNLVFDAEKNIISDFLLSPTWLAIEYLINQNGD
                     KTVLHVELLIILNRQDGITIGFAAHNAERELSRISSQYQEELLNLKDNIYTMLPEISG
                     DNVDRLSKTAVSAVKDRITYNDDYVKDAFRYLKEVKGVTDPNIDLFLAVLDNNEELLK
                     QSIDAGGNLAMDDKDLLKKYEKLLKDHKFVRKPELPA"
     gene            2341..2661
                     /locus_tag="O0X37_02435"
     CDS             2341..2661
                     /locus_tag="O0X37_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61818.1"
                     /translation="MNNEEPTRSGEWNGCTRESTTDWDLYHMLTEDKPVMISELVERS
                     GWNRDVVEASLSRLEDACLIAVMGESVSLLSLSDMLMLNEMMHAPDMPVYIENGVIKA
                     KKQC"
     gene            2655..3209
                     /locus_tag="O0X37_02440"
     CDS             2655..3209
                     /locus_tag="O0X37_02440"
                     /EC_number="2.1.1.206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(56)-2'-O)-methyltransferase"
                     /protein_id="HJK61819.1"
                     /translation="MLKPESYILRIGHRPERDQRVTTHVGLSSRALGASGMYLAADDQ
                     KVADSIADVAHRFGGTYFCENNVKWKSCINNFKRNGGKVVHLTMYGLRLQDVISQIRT
                     EEKILVVVGAEKVPGDMYELADYNVAVANQPHSEISALALFLDHLYEGAELELSFPDA
                     ELEVLPTAVGKTTVKHEEHHGDEA"
     gene            3206..4282
                     /locus_tag="O0X37_02445"
     CDS             3206..4282
                     /locus_tag="O0X37_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HJK61820.1"
                     /translation="MSPSVLVAGYTTRHVAKSAAQQGYDVYAVDHFCDQDLLWCTKDA
                     IAFDELDELPFAIEEMLGRYEIDYVITTSGAELLDVPNRMGTDPTVAARFMDKGKTQE
                     FFESLGVPVPRRLSDGEYPAMMKTLSGAGGWRNAVVRNDEERVCWEEFVEHEPFLMQE
                     VISGIPASVSCVGTGSSAKAIVANEQILRGGESCAYAFSGSVTPCVHPMAKRMMDTAE
                     KIVAASGCVGSVGVDFVLTETEAYAIEVNPRFQGTVETVEAATGLSLFKLHMDACRGI
                     LPEIVPQPRQFCVRKILAAPEPLVLNADMRTLAGTITDIPHPGTMFEEGEVMFSVLGC
                     GASRAEAFTSLDKHITDAIQHIKA"
     gene            4279..4800
                     /locus_tag="O0X37_02450"
     CDS             4279..4800
                     /locus_tag="O0X37_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor"
                     /protein_id="HJK61821.1"
                     /translation="MTAITEEQLQNPAIYQYLLKLVGDEGIELLRRCPNEELSDEDIA
                     AKTEINLNSVRHTLYNLYEHRLAEYRRIKNNETGWLTYLWVMRMDNLNLVLRSEMETV
                     VGKLSARLRYDEANDFYQCKNCGLMTTFNNACTTNFACEQCGEMLVHFDDELLVSALK
                     SRVEKMREALSDA"
     gene            4793..5275
                     /locus_tag="O0X37_02455"
     CDS             4793..5275
                     /locus_tag="O0X37_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HJK61822.1"
                     /translation="MPENQYSQILFSAGCDAGVVRHCEVVALVASRFSGDSVDNDLVR
                     AGSMMHDIGRSVTHDISHAQTGARICSERGEPEELTQIVLRHTGAGLDADECTLLGLQ
                     PIDCVPQTLEEKIVAHADNLVKGSKVISIEERMMRIPDLSTRSKKRIWRLAMEVELLG
                     "
     gene            complement(5285..5845)
                     /locus_tag="O0X37_02460"
     CDS             complement(5285..5845)
                     /locus_tag="O0X37_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 family protein"
                     /protein_id="HJK61823.1"
                     /translation="MGLLTEGITEVIAVTKDNAAPMGIIVKPGMSPRMILFKGSKTVE
                     NILTYGWVTANFVSDCYLYPQYAFSDVEPGDLAEVFVGGIMMQRLLSADAWMAFRTTV
                     LHETEETYYIDLLPVASEYVREDPHPINRGFNSVIDATVHATRYVHSKDEHLRELIEY
                     HLSVVTKCGGPREREAAQLLREICGL"
     gene            complement(5833..>6366)
                     /locus_tag="O0X37_02465"
     CDS             complement(5833..>6366)
                     /locus_tag="O0X37_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HJK61824.1"
                     /translation="GKGIAGATELVKKTTVTDAVLFYKAFGLTQVRVRTEDPMDVNDP
                     ASIQRLKDEQITMYSVMEYSAPHDMVAREWTNGFALTRRAADLLFAQKDGVHAIPAMF
                     LTLMTEYPDTFIAKKFDEATAEEVMYKARAVRDGRQTLAAFDDECIKAGINPGSLADI
                     CIAGIFIALLEGWQWDC"
BASE COUNT         1470 a         1412 c         1750 g         1734 t
ORIGIN      
        1 cgacggggac gacggatgga cgctgctgga tgtgaacgat gatgtggcgg agaagttcat
       61 tgaggcctta aaacatcccg gtattacggt aaaaattcag cgtgatgcag tttcccataa
      121 tctgacggaa tgtgttgact gtggtctgtg catcagcatc tgccagaaaa aggtgttttc
      181 ctttgatgac tcctggaggc tgcaggttaa ctcggaacgt tgtgttctat gcggcaggtg
      241 tgtaatgtac tgtccgcagc gtgcgttaag tctgcagaaa tgattcgcga acacttcgag
      301 tttcgtcaga cgatcactac gatccttgct gatgatcctg cccatatcgc agcggcaaaa
      361 gcagggatgc tttcaggcag agcggacctt gagtcctacc tccggactga tccttttttt
      421 ggaacatcct actcaccggt accggcggag ccggatgccc cgctaattgt acataggatg
      481 gcggacgccg cagaagaggc gtgcgttggg ccgatggctg ctgttgctgc ggcgattgcg
      541 tgggcagggg tggagagtat ggttgtatcc ggtgcttcat ttggcctgat tgacaacggt
      601 ggtgatattg tatttgtagc agccgatcga tccgtacgag ttggtctgta tgccggggca
      661 gcaccggtgt ccgggaagta tgcgtttgta ctgcctcctg cccccgatat ccggagtgta
      721 tgtacatcat ctgcaacggt cggtccatcg gtttcgtttg gtgttgcgga cgcagtggtg
      781 gtgttttctc ataacccggc aaaagcggat gcctgggcaa cagggttgtg taatgccctc
      841 aggccaacag atgaaggaat tgtcctgccg aaaaaagcgg caattgatgg tgtgtatgcg
      901 gttatcgggg agtggaccgc tgcatggggt acagtgccgg agattgttcc ggctgctgtt
      961 cggtatgata tgattacgaa aggcgagtaa aattataccg aacagtgctc gcgtgcaaaa
     1021 gctacggcac gatcgtatgc accgtctccg gtgaaagccg caacgatcct ttccccgtcc
     1081 cacactccaa tgaaaaataa ttttttggat ttatcatatc ccatgatcag ttctgcaagc
     1141 tcaacttgtt gaattctgat tccgtgcaga agttgggatg atggttcaag cagcagaatg
     1201 tcatacacgt cgtcatcggg gatttcattg aaggcgtaca tataggcctg cgctgcattt
     1261 gtcaggtctg atgtacagag ttgtactgca gtatcatagt caaggtcatg catcagccga
     1321 gcaagctctg catcatcgtc attacagcgg ttgtagagat catctgccac aagcgttagt
     1381 tctgcataac tgcacggctg aaccatacgt tatccctcct tttgtagtca ccacttgtcg
     1441 cactcatttg ctgtgtagta ttcgtatttc tcagatgaat aatctatgca ggtagctccg
     1501 gtttcctgac aaatttatga tctttcagga gtttctcgta cttctttaga agatctttgt
     1561 catccatggc aaggtttcct cccgcgtcaa tagactgttt gagtaactcc tcgttgttgt
     1621 ccagaaccgc aaggaacaga tcaatgttgg gatcagtcac cccttttacc tctttgagat
     1681 agcggaacgc atctttgacg tagtcatcgt tgtaggtgat tctgtctttt actgcactca
     1741 cagctgtctt tgacagacgg tccacattgt ctccggaaat ctctggaagc atggtgtaga
     1801 tgttatcctt aagatttagc agttcttcct ggtactgact gctgattctg ctgagttccc
     1861 gttctgcgtt gtgggcggca aagcctatgg tgataccgtc ctgcctgttg aggatgatca
     1921 ggagttctac gtgcagtacg gttttgtcac cgttttggtt aattagatac tctattgcaa
     1981 gccaggtggg acttaagagg aaatcactga tgatattctt ctccgcatca aatactagat
     2041 ttctcatgtc gtttcggtag gaggatttga tgatgatgga tttgagtgcg gtatcaagtt
     2101 ttactagatc ctcttttgtg tcctcacgtg tccggtgttc aaactcctgg agttttctat
     2161 tctgggtgaa tccgtagata ctggagatga tgctgaccac aagggaagta aatgcgacaa
     2221 cgacggtaag ttccatagct atgtctgtca tcttttccag tgataaagga attgtttgtg
     2281 acaagatgcg tgatcctaag catacatcct taatctcttc cgcgtgcaaa ctgttacctc
     2341 atgaacaatg aggagccgac ccgctccggt gagtggaatg gctgtacacg cgaaagcact
     2401 accgactggg atctctacca catgcttaca gaagataaac cggtaatgat ttccgaactg
     2461 gtggagcgat ccgggtggaa tcgtgatgtt gtagaggcat ctctgtcccg gcttgaggat
     2521 gcctgcctta ttgcggttat gggcgagtcg gtgagtcttc taagtttgtc ggacatgctg
     2581 atgttgaacg agatgatgca tgctcccgat atgccggtct atattgaaaa cggagttatt
     2641 aaggcgaaaa aacaatgctg aaaccagaat cctatatttt acgaatcggc cataggccgg
     2701 agcgcgatca gcgggttacc acacatgttg gactgagttc ccgtgccctt ggagcaagcg
     2761 gaatgtatct tgccgcggat gatcaaaaag ttgctgactc cattgcagat gttgcacatc
     2821 ggtttggcgg tacatacttc tgcgagaata atgtaaagtg gaagagctgt atcaacaact
     2881 tcaaacgaaa cggcggaaag gttgtgcatc tgaccatgta tggtctgcgg ttgcaggatg
     2941 tgatctccca aatccgaacc gaggaaaaaa ttctggttgt ggtgggtgct gaaaaagtgc
     3001 cgggcgatat gtatgagctt gcggattaca atgtggcggt tgcaaaccag ccgcactcgg
     3061 agatctctgc acttgcactg tttctggatc atctctatga gggtgcggag ttggaactgt
     3121 cattccctga tgcagagctt gaggttctgc caaccgccgt tggtaagact accgtaaagc
     3181 atgaggagca ccacggggac gaagcgtgag tccgtcggtt ctagttgcag ggtacaccac
     3241 tcgccatgtg gcaaagtctg cagcgcagca aggatatgat gtgtatgcgg tggatcattt
     3301 ctgtgatcag gatctgctct ggtgtacaaa agacgcaatt gcgtttgacg aactggatga
     3361 acttccgttt gcaattgagg agatgcttgg acgctatgag atcgattatg taatcacaac
     3421 gtcgggcgca gagcttcttg acgtaccgaa caggatggga actgatccaa ccgttgcggc
     3481 acggtttatg gataaaggga agacccagga attttttgag tctcttggtg ttccggttcc
     3541 ccgtagattg tctgacggag agtatccggc aatgatgaaa acactgtcgg gtgctggggg
     3601 ctggcggaat gcggtagtgc gcaacgatga agagcgggtg tgctgggaag agtttgtgga
     3661 gcatgaaccg tttctgatgc aggaggttat atccggtatt ccggccagtg tctcctgtgt
     3721 tggaactgga agttctgcaa aagcgattgt ggcaaatgag cagatactcc gcggtgggga
     3781 gagctgtgcc tatgcatttt caggttcggt tactccctgt gtgcatccga tggcaaaacg
     3841 aatgatggac actgccgaaa agattgttgc ggcaagtgga tgtgtgggct cggttggtgt
     3901 ggattttgtt ttaaccgaaa ccgaagctta cgcgattgag gtgaatcctc ggtttcaggg
     3961 aacggtcgaa acggtggaag cagcgaccgg cctaagtctg tttaagctgc acatggacgc
     4021 ctgccgaggt attttaccgg agatcgtacc gcagccccgg cagttctgtg tgcgcaagat
     4081 tcttgcagct cctgaacctt tggttttgaa tgcggatatg cgaactcttg cgggaacgat
     4141 caccgatatt ccacatccgg gcacgatgtt tgaggaaggg gaagtgatgt tttcggttct
     4201 tggatgcgga gcatcgcgtg ctgaagcatt cacatccctg gataaacata ttactgacgc
     4261 catccaacat ataaaggcat gacggcgatc actgaggaac agctgcaaaa tccggcaata
     4321 tatcagtatc ttctgaaact cgttggcgat gagggaattg aactcctccg ccgttgtcca
     4381 aacgaggagc tgagtgacga ggatattgcg gcaaaaacgg agattaactt aaactccgtg
     4441 cgccatacgc tgtataatct ctatgagcac cggctggcgg agtatcgccg gattaaaaat
     4501 aatgagacgg gttggctgac ctatctgtgg gtgatgcgga tggataatct gaatcttgtc
     4561 ctgcgctcgg agatggagac tgtggtggga aagctttcag cacgcctgag atatgatgag
     4621 gcaaacgact tttatcagtg caaaaactgt ggcctgatga cgacgtttaa caatgcctgt
     4681 acgacgaact tcgcctgtga gcagtgtggc gagatgctgg tgcatttcga tgacgaactg
     4741 ctggtttctg ctctgaaaag ccgcgttgag aagatgcgcg aggctctttc ggatgcctga
     4801 gaaccagtat tctcagattc ttttttctgc gggatgtgat gctggtgttg tccggcattg
     4861 cgaggttgtt gccttggttg catctcggtt ctctggagat tcggttgata atgatctggt
     4921 gcgtgcagga tcaatgatgc atgatattgg aagatcggtg acgcatgaca tctctcatgc
     4981 tcagaccggt gcacgtatct gtagtgaacg tggtgaacct gaagaactga ctcaaattgt
     5041 gctccggcat acgggtgctg ggcttgatgc tgatgagtgt actcttcttg gtcttcagcc
     5101 gatagactgt gtgcctcaaa cacttgagga gaagattgtt gcccacgcag ataatctggt
     5161 gaagggatcc aaagtgatct cgattgaaga acgcatgatg agaattcctg atttgtctac
     5221 acgttcaaaa aagagaatat ggcggcttgc catggaagtt gaactgcttg gataatccgt
     5281 ggaattacag tccgcagatc tcgcggagaa gctgtgctgc ctcacgctcg cgtggtccgc
     5341 cgcatttggt caccacactg agatgatact caatgagttc tcggagatgt tcgtctttgc
     5401 tgtgaacata ccgcgttgca tgtactgttg cgtcaatgac actattgaat ccacggttga
     5461 tcgggtgtgg gtcttcccgg acgtactctg atgctactgg taagagatcg atataatagg
     5521 tctcttctgt ttcatgcaga acagttgtcc gaaatgccat ccatgcatct gcagagagca
     5581 gacgctgcat catgattcca ccgacaaaca cctctgccag atctcctggc tcaacgtccg
     5641 agaatgcata ctgcgggtag aggtagcagt ccgacacgaa atttgctgtg acccagccgt
     5701 atgtaaggat gttttcgacc gtttttgatc ccttgaagag gatcattctc ggtgacattc
     5761 cgggttttac gatgattccc atcggtgccg cattgtcttt ggtaacggct atgacctcgg
     5821 tgattccttc agttaacaat cccattgcca tccctccaga agggcgataa aaatacctgc
     5881 aatgcagatg tctgcaagag aacctgggtt tattcctgct ttgatacact catcgtcaaa
     5941 tgcggcaagc gtttgtctgc catcccggac agcgcgtgct ttgtacatta cttcctctgc
     6001 ggttgcttcg tcaaacttct ttgcaataaa ggtgtcgggg tattcggtca tcagggtaag
     6061 aaacatcgca gggattgcgt gtactccatc cttttgcgcg aacagcagat ctgctgccct
     6121 gcgtgtcagg gcaaaaccgt ttgtccattc acgtgcgacc atgtcgtgcg gagcagagta
     6181 ctccatcacg ctgtacatgg ttatctgctc atccttcagc cgctgtatgg atgcaggatc
     6241 gttcacgtcc atcgggtctt cggtcctgac ccgtacctgt gtgagtccaa acgctttgta
     6301 gaagagaact gcatctgtga cggtcgtttt tttcaccagc tctgtagcac cagctatccc
     6361 ttttcc
//
LOCUS       DALUAJ010000113         3084 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_20920,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000113 DALUAJ010000000
VERSION     DALUAJ010000113.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20920"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            577..972
                     /locus_tag="O0X37_02470"
     CDS             577..972
                     /locus_tag="O0X37_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61825.1"
                     /translation="MNLRLGASFIQKFNKYMEGRQETKVHFIRAAMEYWMSVDGDIEK
                     IAERLKQAEQVIALQQERIDELRTFQERCIAYQREIIEEKNKRIQYLTDQLKKSPKAD
                     IYYPENLLEEPTVAEPEPAYQNQDAPTEK"
     gene            complement(1003..2358)
                     /locus_tag="O0X37_02475"
     CDS             complement(1003..2358)
                     /locus_tag="O0X37_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein Srp54"
                     /protein_id="HJK61826.1"
                     /translation="MGLDNLSNSLKDAMKKLAGKTVIDRAAVDELVRDLQRALLSADV
                     NVKLVMQLSQSIKSRALDEDLPKGMNVREHVLRIVYQELVNLVGKEAEFSLRPQKILM
                     AGLQGSGKTTTTGKLCRYFQRKGLKVGAIGADNFRPGAYAQLETLCKKINVPCYGDPK
                     EKDAVKITRDGLAALKDVEVIIVDTAGRHALEDDLIEEIMQINDLLKPDHRWLVIDAA
                     LGQAAREQAKRFHEAIGIDGVIVTKMDGTAKGGGAMSAVSETESGIVFIGNGETIEDL
                     ERFDANGFISRLLGMGDLKALVEKAEEAIKPEDVDVNAMLRGKFTLNDMYKQLEAVQK
                     MGPLKQVLSMLPLGGLNVPSEALDGTADRMKRFRIIMDSMTPQELDDPSLINTSRMTR
                     VAKGSGSSVDEVRELIKYYKMMQKTLKGFRGNRMAMNKMMKQMSKGGGDLGGMGPMGP
                     M"
     gene            complement(2358..>3084)
                     /gene="ftsY"
                     /locus_tag="O0X37_02480"
     CDS             complement(2358..>3084)
                     /gene="ftsY"
                     /locus_tag="O0X37_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HJK61827.1"
                     /translation="VGTHRKIRESPEEVVTNALKHAIEDVLGDGFDLVGYIKAHEKPV
                     KILFTGVNGTGKTTSVAKIAYYLQSQGLSVVVGAGDTFRAGAIEQIRVHCDRLGIKLI
                     AHQEGADPSAVLYDTVEYAKAHNIDVVLADSAGRFHNRVNLMNQLEKIKRVMKPDLVF
                     YVDEAVAGNDAVIRAEEFEKTVSTNGVILTKVDMDPKGGAAISIAYTIGKPLVFLGVG
                     QEYSDMKPFTPALIIDEIFGDEE"
BASE COUNT          853 a          826 c          633 g          772 t
ORIGIN      
        1 atgcaaaagc aggggcatat gcacgtgaac attttgggat gtttgccgga tatgcacagg
       61 aatatctgtt tgcatcccgc gaactcatca gcagaaaagg agaataacca gtgcctgaaa
      121 gactcggtat gagatacaca ggagagacag cacaagcaaa gcgaacctca atacccaaca
      181 ttcaggaaaa ataatcttgt gatatcagaa agcctgtcca acagaaaaac agatctgttg
      241 gccaattcgt ccgacaaaac atgactcccc atcatgtttg gtccaggaat acagactttt
      301 ttatatccgt ggacttgcgg aaacatcttt tctgttgggc agactttttt ccgtcaccgg
      361 agagtaaatg gaaaaattct gaaatacacc agtttccctc cgcaatgcct tgcctggaag
      421 aattattctt tttccctttg caacagacga tcgggacgaa taactatctt ttgactatgc
      481 aaattatacc tattgactat ccatatactt atacctatcc attaggatac ctgttatcta
      541 atgacgggca gtccccggaa acgtccacct gatcagatga acttgcggct cggtgccagt
      601 ttcattcaga aattcaataa gtacatggaa ggacggcagg agacaaaagt ccattttatc
      661 cgtgctgcaa tggagtactg gatgtctgtg gatggggaca tcgagaaaat tgctgagaga
      721 ctaaagcagg cagaacaggt cattgcactt cagcaggaaa gaatcgatga actccgtaca
      781 tttcaggaac ggtgcattgc gtaccagcgt gagatcatcg aagaaaaaaa caaaagaatc
      841 caatacctta cagaccagct gaaaaagagc cctaaagcag acatatatta tcccgaaaat
      901 cttcttgaag agccaaccgt cgccgagccg gagcctgcat accaaaacca agatgcgcct
      961 actgaaaaat aattttttca aaaaagatag tggcaaggag agttacatag gacccattgg
     1021 gcccatgccg ccgagatctc cacctccctt actcatctgt ttcatcatct tgttcatcgc
     1081 catccggttt ccgcggaaac ctttcagcgt tttttgcatc atcttgtagt atttgataag
     1141 ctctcgaacc tcatcaacag aagaaccaga tccttttgcg actctggtca tccgtgatgt
     1201 attgatcagc gagggatcat caagctcttg gggcgtcatc gagtccataa tgatgcggaa
     1261 ccgtttcatc ctatcagcag taccatccag agcttcagag ggaacattca acccaccaag
     1321 cggcagcatg gaaaggacct gttttaacgg ccccatcttc tgcactgctt caagctgctt
     1381 gtacatatca ttcagcgtga acttaccccg cagcattgcg ttgacatcca catcctccgg
     1441 cttgatggct tcttcggcct tttcaacaag tgctttcaga tcgcccatgc caagcagacg
     1501 ggagatgaac ccatttgcat caaaccgctc cagatcctcg atcgtttcac cattaccgat
     1561 gaacacgatt ccagactcgg tctcggaaac cgcagacatg gcacctccac cttttgccgt
     1621 accatccatt ttggtaacga tcacaccatc aataccgata gcctcatgga accgcttcgc
     1681 ctgctcgcgt gcagcctgtc ccagtgctgc atcaatcacc agccagcggt gatccggttt
     1741 gagcagatca ttgatctgca taatctcttc aatgagatca tcctccaatg catgacgacc
     1801 ggccgtatcc acaataataa cttcgacatc cttgagtgcc gcaagaccgt cacgggtaat
     1861 cttcaccgcg tctttctctt tgggatcacc ataacaggga acattgatct ttttgcacag
     1921 agtttccaac tgtgcatagg caccaggccg gaagttatca gcaccaattg ctccaacctt
     1981 cagtcctttt cgctggaagt aacgacaaag ttttccggtt gttgttgttt taccagaacc
     2041 ctgaagacct gccatcaaaa ttttttgggg acgcaggctg aactctgcct ccttaccgac
     2101 gaggttgacc agttcctgat acacaatccg cagtacatgt tcgcggacat tcattccctt
     2161 gggaagatcc tcgtccaacg cccgcgactt aatcgactgt gacaactgca tcacaagttt
     2221 cacattcaca tccgcagaca acagggcacg ctgaagatca cgaaccaact catccactgc
     2281 cgcacgatca ataaccgtct tcccggcaag tttcttcatc gcatccttga gcgaattgga
     2341 aagattatcg agtcccatta ctcctcatcc ccaaaaattt catcaatgat caaagctggc
     2401 gtaaagggct tcatatccga gtactcctga ccaacgccaa ggaacaccag aggtttgccg
     2461 attgtataag cgattgatat tgcggcaccg cctttgggat ccatatccac ctttgtcaga
     2521 atcacaccgt ttgttgaaac agtcttctcg aactcctcag cacgaatcac tgcatcgttt
     2581 ccagcaaccg cctcatccac ataaaagacg agatccggct tcatcacgcg cttgatcttt
     2641 tccaactgat tcatcaggtt gacacggttg tggaaccggc ctgcagaatc agccaaaacg
     2701 acatcaatat tgtgagcctt ggcatactcc acagtatcat aaagtactgc ggatggatca
     2761 gctccctcct gatgggcaat aagtttgata ccaagtctat cgcagtgaac acggatctgt
     2821 tcaatcgccc ctgcccggaa cgtatcacct gcaccaacaa caacagacaa cccctggctc
     2881 tggagataat aagcgatttt tgcaacggat gtggtcttgc cagtaccatt cacaccagta
     2941 aagaggatct ttaccggttt ttcgtgtgct ttaatgtagc caaccaaatc aaatccgtcg
     3001 ccgagaacat cttcgatcgc atgtttaagc gcatttgtaa cgacctcttc tggggattcc
     3061 cggatttttc tgtgggttcc gacc
//
LOCUS       DALUAJ010000114         3422 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_21053,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000114 DALUAJ010000000
VERSION     DALUAJ010000114.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21053"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..150)
                     /locus_tag="O0X37_02485"
     CDS             complement(<1..150)
                     /locus_tag="O0X37_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HJK61828.1"
                     /translation="MSQEYQDVKTGTTTVGIVFQGGVVLATERRATMGTLIASKKAKK
                     VHSITD"
     gene            complement(379..1659)
                     /locus_tag="O0X37_02490"
     CDS             complement(379..1659)
                     /locus_tag="O0X37_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HJK61829.1"
                     /translation="MIEYSDIVIFVILLILSAFFSASEVALVGITRAKVRILSEEGGA
                     AGKRLEKLKNNPDHFLITILVGNNIANVGASAIATAVAFGIFGDAGVAIATGVVTLLL
                     LIFGEIGPKTFATRKTESVALFVATPIYYLTLLLSPFFWVYDRLRRTGKNGTADVPAV
                     TEEEIREWIDVGEMEGAIEQDEKEMIYSVLRFNDTTAREIMTPRPDVAVIEDTASLED
                     AVAHFRDTGYSRVPVFHEHTDNIVGTLNIKDLFNAYTAGNHRAFVKTLMFDVYCVPES
                     KKIDDLLRELQLRRMHMAIVLDEFGGFSGVVTFEDILEELVGDIMDESDTDEVADIIE
                     IGEGLYLVDAQARVTLLNEHFGIALPEDPSNYETVGGLVFSRLGHIPRLGESITIAEQ
                     YITIVVTKMRGRQILKLKLILPQQETDEQDISGE"
     gene            complement(1755..2603)
                     /locus_tag="O0X37_02495"
     CDS             complement(1755..2603)
                     /locus_tag="O0X37_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HJK61830.1"
                     /translation="MKENYLITMIRLGASSMFFHEYQPAEIFSAMYSAGLDHIEFWME
                     TPEFWMQGADISVLENLIEQYPHLAPIAMHAPIFDLNPCSFNPGVAKLSADYTCKTIE
                     MLDQLGGGVVTIHPGKRTAKRPVGPLDRARLTVYLDAVSKSVFGTGVTVAIENMPPAV
                     NAQLVTPDAVRDILDEYAWLSFTWDYAHAQAALPDDPFAFLRECKDRMVNIHASLGTA
                     EMMHMPLSGTSAGEQLRCELAAAKYSGLMTFELEDLNFRKNLDYAEKTSVLAAEAAWF
                     SGLRSI"
     gene            2625..>3422
                     /gene="xseA"
                     /locus_tag="O0X37_02500"
     CDS             2625..>3422
                     /gene="xseA"
                     /locus_tag="O0X37_02500"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HJK61831.1"
                     /translation="MAGKGQQMLLTFCDTEPDERTNTAISPVLENSTLVPHEPEAPAE
                     PAVLSVSELAERIRDAIDTPLLTNICVQGEITGYRPHSSGHMYFSLSEHSDDKATMPC
                     VMWKYAAKTVAFPVKDGILVRATGYVDFYPPYGKMQFVIKKLEPAHAGKTGLYLLKEE
                     WKKQLTEEGVIPRSPCDRRDPPAFPCSVGVVTSRTGSVLQDIRNVLSRRYPLPVLLAH
                     TAVQGEGAHIQIATAIRSLQKKVDVIIVARGGGSFEDLFEFNHPDVVR"
BASE COUNT          929 a          868 c          821 g          804 t
ORIGIN      
        1 atccgtgatg ctgtgtactt tctttgcctt tttgctggcg atgagggtac ccatagtggc
       61 acgccgttcg gtcgcaagga caacaccgcc ctggaaaaca ataccgacag tggtagttcc
      121 cgttttaacg tcctgatatt cctgtgacat ggagacctcg tacttggaca cctgttctgc
      181 tgttccctca aagggctgct atttgcaggt aacgcttgag aatttttgag taaagccgca
      241 aggtataacc ctgctccact ccccgcaccc ggggtcgtga ccgcatgcat atctgcagca
      301 gtgaaaagcg ctattatgta atgtgcatgg gatgagataa agttgccgac cgtgatacga
      361 aaaaaaacga agaaggggtc actccccgct gatgtcctgc tcgtcggttt cctgttgggg
      421 aaggataagt tttagtttga gaatctgacg cccacgcatc tttgtgacga caatggtaat
      481 atactgctcg gcaatcgtaa tcgactcacc gaggcgggga atgtggccca gtcgtgaaaa
      541 aacaagacca ccaactgttt cataattact gggatcttcc ggaagcgcga tcccaaaatg
      601 ctcattgagc agagtaacgc gtgcctgagc atccaccaga tagagaccct caccaatctc
      661 aatgatatcg gcaacctcat cagtatccga ttcgtccatg atatcgccga ccagttcctc
      721 aaggatgtct tcaaacgtca ctacaccgga aaatcctccg aactcatcca ggacaattgc
      781 catgtgcata cggcgcagtt gtaactcacg cagaaggtca tcgatctttt tggattccgg
      841 tacacagtag acatcaaaca tcagcgtctt tacaaacgca cgatgattcc ctgcggtgta
      901 ggcattgaac agatctttga tgttgagtgt tccgacaatg ttgtcagtgt gctcgtgaaa
      961 caccgggacg cgagagtaac cggtatcacg gaaatgggca accgcatcct caagcgatgc
     1021 agtatcctca atcaccgcaa cgtcaggacg gggtgtcatg atctcccgtg cagttgtatc
     1081 attaaaccgg aggacagagt agatcatctc tttctcatcc tgttcaatcg ccccttccat
     1141 ctcacctaca tcaatccact cgcgaatttc ctcctcagtt acagcgggaa catcggcagt
     1201 accattttta ccagtacgcc ggagacggtc atacacccag aaaaatgggg acagcagaag
     1261 agtcagataa tagatcggcg ttgcaacaaa caatgcgaca gactcggtct tccgtgttgc
     1321 aaaggtcttc ggaccgattt cgccgaagat taacagcaac agtgttacca cacctgttgc
     1381 aatagcaaca ccagcatccc cgaagatacc aaacgctacc gcggtagcaa ttgctgaagc
     1441 accaacgttg gcgatattat tgccgacaag aattgtaatc aggaaatgat ccggattatt
     1501 cttcagtttt tcaagacgtt ttcctgcagc ccccccttct tcggaaagaa tgcgaacctt
     1561 cgcacgggta atcccgacaa gtgcaacctc ggatgccgaa aaaaaggcgg acaagatcag
     1621 caggattaca aaaataacaa tatctgagta ttctatcata tatttcacgc cgcaggtgcg
     1681 gcagatctat tccggtgtcc tgagcccgta atcatcattc gcaaatctct ttcttaatat
     1741 atggatgatc gtccttaaat tgatcggaga cccgaaaacc atgcggcttc cgcagcaagt
     1801 acggacgttt tttctgcgta atcaagattc ttccggaagt tgagatcttc aagttcaaag
     1861 gtcatgagcc cagagtattt tgctgcggca agttcacatc tgagctgctc ccccgcagat
     1921 gtacctgaga ggggcatgtg catcatctcg gcggttccaa gacttgcatg gatgttaacc
     1981 atgcgatctt tgcactcacg aagaaacgca aacggatcat caggcagtgc tgcctgagca
     2041 tgggcatagt cccaggtaaa cgaaagccat gcatattcat ccagaatatc ccgcactgca
     2101 tcaggagtga caagctgggc atttactgcc ggaggcatat tttctattgc aacagtaaca
     2161 ccagtaccaa acacagattt agagacagca tccagataaa cagtgagacg cgcacggtcc
     2221 agcggaccaa ccggacgttt tgctgtacgc ttcccgggat gaatggtaac aacgcctccg
     2281 ccaagctgat ccagcatttc gatcgttttg catgtatagt ctgccgaaag ttttgcaaca
     2341 cccgggttaa aggaacacgg attcagatca aaaatcggcg catgcattgc aatgggggca
     2401 agatgcgggt actgttctat cagattctcg agtactgaga tatctgctcc ctgcatccag
     2461 aactccggtg tttccatcca gaactcaata tgatccaacc ctgcagaata catcgcggag
     2521 aagatctctg cgggctgata ctcgtggaaa aacatgctgg atgcgccaag ccggatcatg
     2581 gtgatcagat agttttcttt catccaaata ccaatctatg ggtaatggca ggaaaagggc
     2641 agcagatgct gctgacattc tgtgacacag aaccggatga aaggacaaac acagccatca
     2701 gcccggttct ggaaaactca acacttgtgc cgcatgagcc cgaggcccca gcggagcctg
     2761 cagttctcag tgtgtccgaa ctcgctgaac ggatacgtga cgcgatcgat acacctcttc
     2821 ttactaacat ctgcgttcaa ggtgagataa ccggataccg cccccactca tcaggacaca
     2881 tgtacttctc actgtccgaa cacagtgacg ataaagcaac gatgccgtgt gttatgtgga
     2941 agtatgcagc gaaaaccgtt gcatttccgg taaaggacgg gatactggtt cgtgcaaccg
     3001 ggtatgtgga cttttatccg ccatatggca agatgcagtt cgtgatcaaa aaactcgaac
     3061 ctgctcacgc aggaaagacc gggctgtatc tcctgaaaga agaatggaaa aaacaactga
     3121 cagaggaagg tgtgatcccc agatctccgt gcgatcgtcg tgatcctccg gcatttcctt
     3181 gcagtgttgg agtagtaacc tcacgaacag gatctgtttt gcaggatatc agaaatgtcc
     3241 tgtcacggcg gtatcccctg ccggtccttc ttgcgcacac cgcagtacag ggagaggggg
     3301 cgcatatcca aattgctaca gcaatcagat cacttcagaa aaaagtggat gtgatcatcg
     3361 tggcaagggg aggaggaagt tttgaggatc tgttcgagtt taatcatcct gacgtggtga
     3421 ga
//
LOCUS       DALUAJ010000115         1667 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_21204,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000115 DALUAJ010000000
VERSION     DALUAJ010000115.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21204"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="O0X37_02505"
     CDS             complement(<1..893)
                     /locus_tag="O0X37_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HJK61832.1"
                     /translation="MADPNLYLKGGVIPERDNEHYIIRLRTPAGMLGVDALAGIAEIA
                     KKYGIQDTHLTTRQTIELLHVGPEILDDLLADLEKNGTPIGAERNEVVNVTACLGNAN
                     CKYSVIDTISLAKKLDEKHFGKEMPVKVRIAISGCPNGCTSERLNEIGITGLRRPVRN
                     EGLCTGCGTCGYYCREHAILIENGKLRLNQGDCMLCGFCIHACPFQYINFEDPLYLIT
                     LGGRRGRHPKIGRTFYIARSEDDVVEIVGKIIYWIFRSIASDKMLPEQLTDEEFAEFR
                     EKVVKSLKPLGIDGVDPTNAY"
     gene            complement(1012..1491)
                     /locus_tag="O0X37_02510"
     CDS             complement(1012..1491)
                     /locus_tag="O0X37_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HJK61833.1"
                     /translation="MTLPSYFSIPDTLDSAQLKVQAEELFNSGQFYCSEAVVKIIRDT
                     FCPSVPDAIIATASGFPVGMGGGGCTCGAVAGGVMALGLVFGRTKPEEKVQSAECMKY
                     ARELHDRFRKENKAICCRILTKNMLEDSSEHKKQCAELTGEVVEIVAEIILREARAQ"
     gene            complement(1488..>1667)
                     /locus_tag="O0X37_02515"
     CDS             complement(1488..>1667)
                     /locus_tag="O0X37_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HJK61834.1"
                     /translation="QFQLGTQPLVGAYRVTFNVTSAQATDEEIESLKEAAYNGCMALF
                     TVRNAIPLTVSAKRA"
BASE COUNT          399 a          398 c          364 g          506 t
ORIGIN      
        1 cagtaagcat tagttggatc cacaccgtca atgccaagcg gtttcaaact cttcacaact
       61 ttttcgcgga actcagcaaa ctcctcatca gtaagttgtt ctgggagcat tttatctgat
      121 gcgatacttc tgaaaatcca gtagatgatc tttcccacga tttccacaac atcgtcctcg
      181 gagcgtgcga tatagaatgt tcttccgatc tttggatgac gtccgcgtct accaccaagt
      241 gtaattagat agagaggatc ctcgaaatta atatactgga aaggacatgc gtgaatgcag
      301 aatccacaga gcatacaatc tccctgattt aaccgaagtt ttccgttttc aatcaggatt
      361 gcatgttccc ggcagtagta tccgcaggtt ccgcatccag tacaaagccc ctcattacgt
      421 actggccgac ggagtccggt aatgccaatc tcatttaatc gttcactcgt acacccgttc
      481 gggcagccgg agatcgcaat gcgaactttc acaggcatct cctttccgaa atgtttctca
      541 tcaagttttt tggcaagtga gatggtgtcg ataacggagt acttacagtt tgcatttcca
      601 aggcatgcag tgacatttac gacttcatta cgttctgcac cgatcggagt cccgtttttt
      661 tcaagatccg caaggagatc atcgagaatt tcagggccta cgtgaagcaa ctcaattgtt
      721 tgtcttgttg ttaagtgagt gtcctgaatt ccgtatttct ttgcgatctc ggcaattcct
      781 gcaagagcgt ccacaccaag cataccggca ggtgttctga gccggataat atagtgttcg
      841 ttatcccgtt ctgggatcac tccgcctttt agatagaggt tcggatctgc cattgttgta
      901 cctcgatcat tggagagggg ataataaaag aattgtcttt gcagcacctg tttcaaacag
      961 tttgttctgg ttgaaaaata gcaaagcgaa gatgactctc agagacttct tttactgtgc
     1021 tctggcttcc ctgagaataa tctcggcaac aatttcaaca acttccccgg taagttctgc
     1081 acactgtttt ttgtgctctg atgagtcctc aagcatattt tttgtcagta tcctgcagca
     1141 gattgctttg ttttctttcc ggaatctgtc gtgtagttcg cgtgcatatt tcatacactc
     1201 tgcggactgt actttttctt cgggtttggt tctgccaaag acaagtccaa gagccattac
     1261 tcctcctgcc actgccccgc atgtacatcc acctccgccc atacctacag ggaaccctga
     1321 agctgtggcg atgattgcat ccggaactga tgggcagaat gtgtctctga ttatttttac
     1381 tactgcttcc gagcaataaa attgtccgct gttgaaaagt tcttctgcct gaacttttaa
     1441 ttgtgcactg tcgagtgtat ctgggattga aaaataggaa gggagtgtca tgctctcttt
     1501 gcagagacgg ttaacgggat tgcatttctt accgtaaaca gtgccatgca cccattatat
     1561 gctgcttcct tgaggctttc gatctcttca tcagttgcct gtgcgctggt tacattgaag
     1621 gtaactctgt atgcaccaac cagaggctgt gttccgagct ggaactg
//
LOCUS       DALUAJ010000116         3814 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_21296,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000116 DALUAJ010000000
VERSION     DALUAJ010000116.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21296"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="O0X37_02520"
     CDS             <1..671
                     /locus_tag="O0X37_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJK61835.1"
                     /translation="SGMSEAFRAVGKKTNVIVYAGDGGTADIGIQALSGALERGEDFL
                     YICYDNEAYGNTGMQRSGATPLGAITTTTPNGKTVNKKDLDRIIEAHNLPYQATACAS
                     YPADIYNKVKKALSIPGPKFMHILAPCPPGWRTPSEKTVEIGKMAVKSGIWVLWEKEY
                     DKFTISAPSRAAMKKPAPVIDYLTAQGRFRKVDEATAARIQAQVDKNLKKVAAEAAYS
                     EENA"
     gene            668..1798
                     /gene="porA"
                     /locus_tag="O0X37_02525"
     CDS             668..1798
                     /gene="porA"
                     /locus_tag="O0X37_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HJK61836.1"
                     /translation="MSDKLVMSTGNKAMASAVKMAKPTVVAAYPITPQTEVIESIADY
                     VESGAMDAKYIPVESEHSAMTACIGASITGVRVFTATSSHGLLYMHEMLHWAAGARLP
                     IVMGQVNRTLAPGWNIWAEHSDALSQRDTGWLQVYVSTVQEAYDATLMAFRIAEDNRV
                     LLPVMINMDGFLLSHIMQPFEMTEPGDFIPPINLPHKIDVNNPMGYGAMSPSDIHFTF
                     RHDMEKAMKNARTVIAETEAEFAKRFGRSYAPVEEYLCDDADVVIVAMGTLGKEAEVA
                     ADLLRKEGIKAGVMRIRWLRPFPLDMKIEGKELVVIDRDYSFGFGGVIAGEIQARYPD
                     AKIARVIAGLGGQEVTYEDMAELVRTRKIGTEFWFGIPEEEQ"
     gene            1799..2326
                     /locus_tag="O0X37_02530"
     CDS             1799..2326
                     /locus_tag="O0X37_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HJK61837.1"
                     /translation="MYEIRIHSRGGQGGVTAARMMAAAAVKDGKFATACPFYGAERRG
                     APIVSFVRIDDHPVRIYSQIKKPDMIIVLDPTVMETVNVLDGLKDGGAIFINTHENVQ
                     FPDKFTVHKADLTGIALSENLVVAGSPILNTPVIGALAKLGLFTPESGEAAITDTFTD
                     TRNVTVAKKAYDEVI"
     gene            2327..2578
                     /locus_tag="O0X37_02535"
     CDS             2327..2578
                     /locus_tag="O0X37_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK61838.1"
                     /translation="MTMPKMTISTPKEGAMGQTGSWRTFRPVVNREICNHCGICAMYC
                     PDAVIDPDNEEIDLVYCKGCGICANECPKKCIEMVREEH"
     gene            2743..>3814
                     /gene="aspS"
                     /locus_tag="O0X37_02540"
     CDS             2743..>3814
                     /gene="aspS"
                     /locus_tag="O0X37_02540"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050560 - aspartate-tRNA(Asn) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HJK61839.1"
                     /translation="MRIPIKDVTPDCERAEIAGWVHEERDLGGLTFLLVRDRTGILQV
                     TIPKKKVSPEVLAAVKEVSRESVISCEGVVKATEKAPGGRELVPDTLKVISRADTPLP
                     LDVSEKVPADLDTRLDNRYLDLRKPRVNAIFLIRNAALRAINEFLWDHHFTQVQTPKI
                     VAAATEGGTELFPLAYFDKEAFLNQSPQLYKQMLMSAGFDRVFEIGPIFRAEEHNTVR
                     HLNEATSIDIEMSFADEQDAMKVLEETVSYAYRKVADTCSNELAVLGLNDFEVPATPF
                     PKITYQEAIDIATAKGEALVFGDDLTTAAEKIVGDEMGTMYFITEWPSSTRPFYTMPF
                     EDRPEVCRAFDMMHPRMELSSGA"
BASE COUNT         1021 a          978 c         1030 g          785 t
ORIGIN      
        1 cctcgggaat gagcgaggcc ttccgtgcag ttgggaagaa gacgaatgtc atcgtctatg
       61 caggcgatgg aggaaccgct gatattggta ttcaggcact atccggtgct ctggagcggg
      121 gagaggactt cctctacatc tgctacgaca atgaggcata tggaaacacc ggtatgcagc
      181 ggtcaggagc aaccccgctt ggtgcaatta caacaacaac gccaaacgga aagaccgtca
      241 acaaaaaaga tcttgacaga atcattgagg cacacaacct tccatatcag gcaacagcct
      301 gtgcatcata tcctgccgac atctacaata aagtaaagaa ggcactctcg attcccggac
      361 caaagtttat gcacatctta gcaccctgcc cgcccggatg gagaactcca tcggaaaaga
      421 cggttgagat cggaaagatg gcagtaaaat caggtatctg ggtgctctgg gaaaaggagt
      481 acgacaaatt taccatcagt gccccgtcgc gtgcagcgat gaaaaaacct gcaccggtga
      541 ttgactacct gacagcccag ggaagattcc gcaaggtgga tgaggcaacc gctgcacgga
      601 ttcaggcaca ggttgacaag aatctgaaga aagttgcagc agaagcggca tactcggagg
      661 agaacgcatg agcgacaaac tggtaatgtc aaccggtaac aaggcaatgg catctgcagt
      721 gaagatggca aagccgaccg tggttgcggc atatccgatc acaccacaga ccgaggttat
      781 tgagtcgatt gcagattatg tcgagagcgg tgcaatggat gccaagtata tcccggtcga
      841 atcggaacac tctgcaatga ccgcctgtat cggtgcatca atcacaggcg tccgtgtgtt
      901 tacggcaaca agttcgcacg gtcttctgta catgcatgag atgctgcact gggcagcagg
      961 cgcacggctt ccgattgtga tgggacaggt aaaccgtaca cttgcgcctg gatggaacat
     1021 ctgggctgaa cactcggatg ctctctccca gcgtgatacc ggatggctac aggtgtatgt
     1081 ttcaacggtc caggaagcat acgatgcaac actgatggcc ttcagaattg ccgaggataa
     1141 cagagtgctg ctgccggtga tgatcaacat ggacggattc ctgctctctc acatcatgca
     1201 gccgttcgag atgacagagc caggagattt catcccgcca atcaatctgc cgcacaagat
     1261 cgatgtgaac aacccgatgg gatacggggc aatgtcgccg tctgatattc acttcacctt
     1321 ccggcatgat atggagaagg caatgaagaa tgcccgtacc gtgattgccg aaacagaggc
     1381 agagtttgca aagcggttcg gcagaagcta tgcaccggtc gaagagtatc tctgtgatga
     1441 tgcagatgtt gtgatcgttg caatgggaac tcttggaaaa gaggctgagg ttgcagcgga
     1501 tcttctccgc aaagaaggta tcaaagcagg tgtgatgcgc atccgctggc tccggccgtt
     1561 cccgcttgac atgaagatcg aaggaaagga actggttgtg atcgaccgtg attactcgtt
     1621 cggattcggt ggtgtgatcg caggagagat ccaggcaaga tatccggatg caaagattgc
     1681 acgggtcatc gccggtcttg gcggtcagga ggtaacatac gaggatatgg cagagcttgt
     1741 ccgcacacgc aaaattggaa ccgagttctg gttcggcatc cccgaggagg aacagtaaat
     1801 gtacgagatt cgtatccatt cacgcggcgg acagggtggt gtgaccgctg cacgcatgat
     1861 ggcagcagca gcagttaaag acggaaagtt tgcaacggca tgcccgttct acggcgcaga
     1921 gcgccgtggt gcgccgatcg tctccttcgt gcggattgat gatcacccgg tcaggatcta
     1981 ctcacagatc aaaaagccgg atatgatcat cgttctcgat ccgacggtga tggaaaccgt
     2041 aaacgtactg gacggtctca aagacggagg agcaatcttc atcaacaccc atgagaacgt
     2101 gcagttccct gacaagttca cagtgcacaa agcggatctg acaggaatcg cgctttcaga
     2161 gaatcttgtt gttgccggaa gcccgattct gaacacgccg gtgatcggag ctcttgcaaa
     2221 acttggtctg ttcacgcctg agtcgggaga ggcagctatc acggatacat ttacggatac
     2281 ccggaatgtg acggttgcaa agaaggcata cgatgaggtg atctaaatga caatgccgaa
     2341 gatgacaatc agtactccaa aggaaggggc aatgggacag accggttcct ggagaacatt
     2401 ccgcccagtg gtaaaccgcg agatctgtaa ccactgcggt atctgtgcga tgtactgtcc
     2461 tgatgcggtg atcgatcccg acaatgagga gatcgatctg gtgtactgca agggctgcgg
     2521 gatttgtgca aacgagtgcc cgaagaagtg catcgagatg gtacgagagg agcactaagg
     2581 cttctttctg attttctttt tttctcagtt gtgttctgaa aatgtatcaa gggaaaagag
     2641 ttctcccctg atgccattat cacagaaaga cgggtaactt tacaacagca tgaggcagga
     2701 caaacactaa tctatccaga ccgcaaataa catagagatt ttatgcgcat cccaataaaa
     2761 gacgtcaccc ccgactgtga gcgggcagag atcgccggat gggttcacga agagcgtgat
     2821 ctcggcggtc tcaccttcct cctggtccgc gacagaacag gaattctaca ggtaaccatc
     2881 cccaagaaaa aagtcagccc cgaagtgctc gccgcagtca aagaagtaag cagagagtct
     2941 gttatctcct gcgaaggagt tgtcaaggca accgaaaaag cacccggagg acgggaactt
     3001 gtccccgata ccctcaaggt tatctcccgc gcagacaccc cgctgccgct ggatgtctcc
     3061 gaaaaagttc cggcagatct tgacacccgc ctcgacaacc gttatcttga cctgcgcaag
     3121 ccccgcgtca acgcaatctt tctaatccgt aacgcagccc tgcgtgcgat caatgagttc
     3181 ctctgggacc accacttcac ccaggtgcag acaccaaaga tcgtcgcagc cgcaaccgaa
     3241 ggcggaacag agcttttccc ccttgcatac ttcgacaagg aggcattcct caaccagagc
     3301 cctcagctct acaagcagat gctcatgagc gcagggttcg accgtgtctt cgagattggt
     3361 ccaatcttcc gcgcagagga gcacaacacc gtccgtcact taaacgaagc aacctcgatt
     3421 gatattgaga tgtcctttgc cgacgaacag gacgccatga aagtactcga agagaccgtc
     3481 tcatacgcat accggaaggt tgccgatacg tgcagcaacg aacttgccgt ccttggtctc
     3541 aacgacttcg aggttccagc aactcccttc cccaagatca cctatcagga agcaatcgac
     3601 attgcaacag caaaaggtga agcccttgtc ttcggtgatg atctcaccac cgccgcagag
     3661 aagatcgtcg gtgacgagat gggcacgatg tacttcatca ccgagtggcc gtcctcaacc
     3721 agaccgttct acaccatgcc gtttgaggac agacctgagg tctgcagagc atttgatatg
     3781 atgcacccga gaatggagct ttcctccggt gccc
//
LOCUS       DALUAJ010000117         2969 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_21489,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000117 DALUAJ010000000
VERSION     DALUAJ010000117.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21489"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="O0X37_02545"
     CDS             <1..270
                     /locus_tag="O0X37_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61840.1"
                     /translation="ILQYNAMDTYPHWGGPFPTYVYLPDSLSGMNVSVVEILTSAKVK
                     RATEQFPMSSGMTYYLESHVIIPGENVSGWIPIPGFSRNVPWDRF"
     gene            866..1795
                     /locus_tag="O0X37_02550"
     CDS             866..1795
                     /locus_tag="O0X37_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61841.1"
                     /translation="MDRETYRSLLQRYLTTCDRYTLSTHTSGSLEDPESVSYPCNEET
                     IRVLEQYENFLSLGTQTSIAFYYQDTCVCYTIPDRELCIIDTLFLHDPHVVLKPFYQP
                     LRRAYREYCTDYQHCLGKGELYSLQDTTHTCYLQYLEKLFSSCHTFMLVNHDPHTPEK
                     LSQIQEALIRFEISKNIPVTYPGVWEYTQNITTHIYECCPESMQILEKLDNFFSMGEH
                     TDIVFSRYDKCVLYTLSDEKLCIIDLWTLGETQPGLQLSPVVKIDNSEISEPALFQIM
                     NAAGVASRAGLERYLLHAGHTWGTPDSTERASK"
     gene            1908..2327
                     /locus_tag="O0X37_02555"
     CDS             1908..2327
                     /locus_tag="O0X37_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61842.1"
                     /translation="MLSRNTKYILMILVVIGFIGIGLVIEQSSAIGFSLLAYCLGLVI
                     GLRSAVALISLNTGKTKSFPDVTLKKSGYAVIAVLILLFAAIAGWTAYCLGCFMQEGF
                     ETAVMAYIAGFGISCIAVSTCFYLQRYLPHEENPDET"
     gene            complement(2336..>2969)
                     /locus_tag="O0X37_02560"
     CDS             complement(2336..>2969)
                     /locus_tag="O0X37_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HJK61843.1"
                     /translation="VDNREMSSKVAEHLSNFGAKITLAALPVGDYAIGDRVLVERKTA
                     QDFVDTLVDRDLFGQIRGLAESASRPVLIIEGGSIADLYELRNIHPNAIRNTLASIAV
                     DYDVSILFTRDAEETAGMLYAFARRELGEAKSERSFHRHKSARSGREELEYILTAVPE
                     VGPKAAREILSAFGTLRAVFSASEEELMRIKGVGEKTAGGIVETAMRQYR"
BASE COUNT          809 a          772 c          650 g          738 t
ORIGIN      
        1 attcttcagt acaatgcaat ggatacgtat ccacactggg gcggaccgtt tcccacgtat
       61 gtgtatcttc cggactcact ttccggcatg aacgtgtctg ttgttgaaat attgacttca
      121 gcaaaggtaa aaagagcgac cgaacagttt ccgatgagtt ctggtatgac gtactacctg
      181 gaaagccatg taatcattcc gggagaaaat gtgtccggat ggattccgat accaggcttt
      241 tcccggaatg ttccctggga tagattctga tgcctctcat tcatcattat cacagaactc
      301 agatcagaca aaatactcct ggaggtgacc aagtatgatg tactcagaaa aacgatgttt
      361 ggaaatctat cgtgtagcga gagaaaaaaa tctcttctac tccgcacacc agcgtgatca
      421 tgattcctat gagctggttc ttgattattt actcaggcag tgtgacaggt tttctcttga
      481 aatagatgtt atctataaaa acaaccaaaa aacgaactgg aatgaacctc agatttctgc
      541 aaagctctct ccctattttc tggaaaagcg gtgtgttgag acagaatctg catggcccag
      601 aaaaaataac agcataacca cccagttcaa agagatctca cactatcggt gttgtgagga
      661 atccatagca atacttaagg aatacgaaaa tttcctcagc atcgacgatc tgctggacat
      721 ctgtttcttc acaaaagaaa agatgattct ccacataatt tctcatgaag atcagtatct
      781 catggacccg aattttttcg ctgacttccc ggagtttctg gaaagagaaa aaacactctt
      841 tacagagagc cgtcatctca gagaaatgga tcgcgaaaca tatcgttcgc ttctccaacg
      901 ctatcttacc acgtgtgatc gctatacact cagtacacat acatctggat cgctggaaga
      961 tccagaatct gtttcatatc cctgtaatga agagacgatc cgtgttcttg aacagtacga
     1021 aaattttctc tcacttggca cgcagacatc catcgccttc tattatcagg atacatgtgt
     1081 ctgttatacc attcctgaca gagaactctg cattattgat acgctgtttc tccatgatcc
     1141 gcatgtcgtc ctcaaaccct tttaccaacc actgaggcgt gcatatcgtg agtattgtac
     1201 agattatcaa cactgtcttg gtaaaggaga actctattca cttcaggaca caacacatac
     1261 atgctatctc cagtatctgg aaaaactgtt ttcctcctgc cacacattca tgctggtaaa
     1321 ccatgatccc catactcctg aaaaactctc acagatacaa gaggcactta tccggtttga
     1381 gatctccaaa aacatacctg tcacgtatcc gggggtatgg gaatatacac agaacatcac
     1441 aacacacatc tatgaatgct gcccggaatc catgcaaatt ctggaaaagc tggataattt
     1501 tttctcaatg ggagaacata ctgacatcgt tttctccagg tatgataaat gcgttctcta
     1561 tactctttca gacgaaaaac tttgcataat tgatctctgg acgcttggtg aaacacagcc
     1621 cggtctccag ttatctccgg tagtcaaaat agataatagt gagatttcag aaccggcact
     1681 tttccaaatc atgaatgctg ccggtgttgc atcacgtgca ggattggaga gatatttact
     1741 gcatgcaggt catacctggg ggactcctga ttccacagag cgtgcatcga aatgagtgcg
     1801 tccctgcctg acaaattatc agcagaacgg gcagggagag acgagagtcg gaaaaacatc
     1861 agatcatccc tgacagtagt cagtatacca aacggcggga tctccccatg ctcagcagaa
     1921 acacaaaata catactcatg atcctggtgg tcatcgggtt catcggtatc ggtcttgtga
     1981 tcgaacaatc atccgccatc ggattttcgc tgctggccta ctgtttgggt ctggtgatcg
     2041 gactgagaag cgcagttgca ctcatcagcc ttaacacggg gaaaaccaaa tcgttcccgg
     2101 atgtaacact aaaaaagtcc ggatacgccg tcattgcagt actaattctc ctctttgccg
     2161 ccatcgccgg atggaccgcg tactgtctcg gatgtttcat gcaggagggt tttgaaacgg
     2221 ctgtcatggc gtacatagca gggtttggaa tcagctgcat cgcagtcagt acatgcttct
     2281 atctccagcg gtatctgccg catgaggaga acccggacga aacctgagag atacctcagc
     2341 ggtactgccg cattgccgtc tccacaatac cgcccgctgt cttctccccg acacctttga
     2401 tccgcatcag ctcctcctcc gatgccgaaa acaccgcccg gagtgtcccg aacgccgaaa
     2461 gaatctcgcg ggcggctttc ggccccacct cgggaacagc ggtcagaata tactccagct
     2521 cctcacgacc gctgcgtgcg gacttgtgac ggtggaaact ccgctccgac tttgcctccc
     2581 caagctcccg gcgggcaaac gcatacagca ttcccgccgt ctcttccgca tccctggtaa
     2641 aaagaatgga gacatcataa tccaccgcaa tcgaggcaag cgtgttgcgg atcgcattcg
     2701 gatggatgtt gcgcagctca tacagatccg caatcgaacc gccctcgatg atcagaaccg
     2761 gacgggacgc cgactcggca agaccgcgga tctggccgaa gagatcgcgg tcaaccagtg
     2821 tatccacaaa gtcctgggcg gtcttgcgtt ccaccagcac acgatcaccg attgcataat
     2881 caccgaccgg aagagccgca agcgtgatct ttgcgccgaa gttgctgaga tgctccgcaa
     2941 cctttgagga catctcacgg ttatccacc
//
LOCUS       DALUAJ010000118         2402 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_21527,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000118 DALUAJ010000000
VERSION     DALUAJ010000118.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21527"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /gene="uppS"
                     /locus_tag="O0X37_02565"
     CDS             <1..660
                     /gene="uppS"
                     /locus_tag="O0X37_02565"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HJK61844.1"
                     /translation="RYAKAHGKDTGYGHRLGADTTERVLDWMGELGIRHVTFFAFSTE
                     NFRRPEEEKEELHQLFMEKFSALLTDERVSQYGINVSVIGDRSLLDAELLQIIEKAEA
                     ATKNNSRYFVHIALAYGGRNEIVATARKIVAAVRDGSISADAITPRLVTENMYQGISM
                     PPVDLIIRTANDRRTSNFLPWMANGNEAAVCFCTPTWPEFRYVDMLRALRVYDCRMRE
                     S"
     gene            635..1111
                     /locus_tag="O0X37_02570"
     CDS             635..1111
                     /locus_tag="O0X37_02570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJK61845.1"
                     /translation="MTAACVKAEGKNMECVIKHFSELSRDELFEIYKLRVAVFVVEQQ
                     SPYPEVDDADPVAYHIWFKDQEGILAYARVLPAHTVCETVSIGRIIAVKRRCGLGTKI
                     VAEAIRVAEEMFGAETITITAQTYAKEFYEKSGFVQCSEEYLDAGVPHIKMIRKRK"
     gene            complement(1120..1728)
                     /locus_tag="O0X37_02575"
     CDS             complement(1120..1728)
                     /locus_tag="O0X37_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HJK61846.1"
                     /translation="MTKKVLALFGSNILGGNTDKLLSAAIKGVEDAGCTVDRINVAHI
                     HAGPCNQTYSCMIEPTCAIEDDMQDAYRKIKQADSIIIATPVMTYGIPGALKSFMDRC
                     QPFYMAKYYRKQPFITPEHAKIRRTLFLCIGGMNAPDVFTGPVLTTKAFCAIVDAKYA
                     DELLQNDMDTIVNLDNKPEILAEAYEKGKALGERIVKMRDGE"
     gene            1919..2296
                     /locus_tag="O0X37_02580"
     CDS             1919..2296
                     /locus_tag="O0X37_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HJK61847.1"
                     /translation="MTEKTHTCGCHNGTGDMKELEKNIGHVPVFFRDLAESDPGMHEA
                     VLKLDNYIWADGALSRKQKKLMAIAIAAAMRDDHALHAQMQGAKKLGVTIEEVDEALR
                     VAFMLAGMPSYVYGKTEAAEIFK"
BASE COUNT          635 a          555 c          624 g          588 t
ORIGIN      
        1 aggtatgcca aagcccatgg aaaagatacg ggatacggac acagactcgg tgccgacaca
       61 acagaacgtg tgcttgactg gatgggagaa cttggcatcc gccatgtgac tttttttgca
      121 ttttcaaccg agaacttccg ccgtcctgaa gaggaaaaag aggaactgca ccaactgttt
      181 atggaaaagt tctctgctct tctaacggat gagcgggtat cccagtacgg gatcaatgtt
      241 tcggtgatag gcgatcgatc actgctggat gcggaacttc ttcagataat tgagaaggca
      301 gaggcagcaa caaagaataa ttcccgctac tttgtacaca ttgctcttgc atacggaggc
      361 cgcaacgaga ttgtggcgac tgcgagaaaa attgtagctg ccgttcggga tggatcgatc
      421 tccgcggatg caataacacc cagactggta acggaaaata tgtatcaggg aatttctatg
      481 ccacctgtag atctaattat ccgtacggca aacgatcgcc ggacctcgaa ctttttaccg
      541 tggatggcaa acggcaacga ggcggcggtc tgcttctgta cgccgacatg gccggagttc
      601 cggtacgtgg atatgctgcg tgctcttcgt gtgtatgact gccgcatgcg tgaaagctga
      661 agggaaaaac atggagtgtg tgatcaaaca tttctcggag ttgtcccgcg acgaactatt
      721 tgagatctac aaactcaggg ttgcggtttt tgtcgtggaa caacagtctc cgtatcctga
      781 ggtggacgat gcggatccgg ttgcctacca tatctggttt aaagatcagg aagggattct
      841 ggcatatgca cgggttctgc ctgcacatac agtgtgcgag acggtatcta ttgggaggat
      901 aattgcagtt aaacgcagat gcgggctggg tacgaagata gttgccgaag cgatcagggt
      961 ggcggaggaa atgtttggag cagaaacaat aacgatcact gcacagacct atgcaaagga
     1021 attttacgag aaatcaggat ttgtacagtg ctccgaagaa tatcttgatg caggggttcc
     1081 gcatatcaaa atgatacgga agagaaagta agagagggat tactctccgt cacgcatttt
     1141 tacaatacgt tcaccgagcg cttttccttt ttcgtatgcc tcagcaagaa tctccggttt
     1201 gttatcaaga ttgacgatgg tgtccatatc gttttgcagc agttcgtccg cgtattttgc
     1261 atccacaatt gcgcagaatg ctttcgtggt caggaccgga ccggtaaaga cgtcgggcgc
     1321 attcattcct ccgatacaca gaaagagtgt gcggcggatc tttgcgtgtt ctggcgtgat
     1381 aaacggctgt ttacgatagt actttgccat gtagaacggc tggcatcgat ccataaagga
     1441 ctttaatgcc cccggaatgc cgtaggtcat caccggggtt gcgatgataa tgctgtccgc
     1501 ctgtttgatt ttgcggtagg catcctgcat atcatcctca atagcacagg tcggttcaat
     1561 catacaggag tacgtctggt tacagggtcc ggcatggata tgggcaacat tgatccggtc
     1621 aaccgtacaa ccggcatcct ctacaccttt gattgctgca ctcagcagtt tgtcggtgtt
     1681 tcctccgagg atattgctcc cgaacagcgc cagcactttt tttgtcatta cgtggtttat
     1741 gtgtcagcca aatataaaaa tgaggtgatg ggtccaaatt accgggaaaa gccatcaaga
     1801 acagacaaca aacctggaaa ctcccgggtg ggagatccca caggatttta tctccgatgt
     1861 caccacctaa aagactaata gcaaaaccgg ctgatatatt ggatcagtga aaatatccat
     1921 gacagaaaaa actcatacct gcggctgcca caacgggacc ggcgacatga aagaactgga
     1981 aaagaacatc ggccatgttc cggtattctt ccgtgatctt gccgagtcgg atcctggaat
     2041 gcatgaagca gttctcaaac ttgacaacta catctgggcg gacggggcac tctcccgcaa
     2101 acagaagaaa ctgatggcaa tcgccattgc agctgcaatg cgtgacgatc atgcactcca
     2161 cgcacagatg cagggagcaa agaaactcgg cgttaccatt gaagaggttg acgaggcact
     2221 gcgtgttgcg ttcatgctcg cgggtatgcc gtcttatgtg tacggtaaga ccgaggcagc
     2281 agagatattc aaataaacct gatttttgcg ggagcctgca cacctccccc tctaaacttt
     2341 catcatcttt tccgtcctaa acagatagta tgaatgatct cctgccgctt gcagtctccg
     2401 ca
//
LOCUS       DALUAJ010000119         1787 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_21535,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000119 DALUAJ010000000
VERSION     DALUAJ010000119.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21535"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            2..1510
                     /locus_tag="O0X37_02585"
     CDS             2..1510
                     /locus_tag="O0X37_02585"
                     /EC_number="2.4.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP phosphorylase"
                     /protein_id="HJK61848.1"
                     /translation="MVDIATVIVLLHADDARQIGVREGDRVTILNTPKGTSVRAPVAI
                     TKTLITPGQVTISSGVNKRLQIEDGESVEIRASPRPQSIEFIRKKMDGGKLTREETAQ
                     IVEDMTKEVLSPSEITAYITAAYINGLDMNEVEHLTREMVASGDRLTFNTRPIVDKHS
                     IGGVPGNKITLLVVPVIAAAGLLIPKTSSRAITGAGGTADLMEALAPVTFTVQEIQQM
                     TLKAGGVIVWGGATNIAPADDMIITYEYPLKIDARGQMLASIMAKKMAVGSDTCVIDI
                     PVGPGTKVSDEAEGRVLASELIELGRRLGIRVECAITYGGAPVGRNIGVNLEVAEALR
                     ILEGESGPNSLMQKSAALAGMALEMTGKTAYGYGAEAAMDLIRSGKALAKMRDIIEVQ
                     GGDPKIKSSDLVFGSNVFDVPAPNDGYVVSVKNRDLINIARTAGSPADHGAGLHLHKK
                     PGEAVKKGEPILTIYAERGWRLQRAIEEARVSMPVVVEGMLLDRVPPIRRVA"
BASE COUNT          428 a          396 c          524 g          439 t
ORIGIN      
        1 catcgttgac attgccacag tgattgttct gcttcatgca gatgatgcac ggcagattgg
       61 tgtccgtgaa ggggatcggg ttacaatatt aaatacaccc aaagggactt ctgttcgtgc
      121 ccccgttgca ataacaaaga cgctgataac gccgggacag gtaacaatca gttccggcgt
      181 caataaacgc ctccagatag aggacggcga gtccgtagag attcgtgcat caccccggcc
      241 ccagtcgatt gagtttatcc ggaaaaagat ggatggggga aagctgaccc gtgaggaaac
      301 cgcacagatc gtggaggaca tgaccaagga ggtactttct ccctctgaga tcacggccta
      361 cattactgct gcctacatca acggccttga tatgaatgag gtggagcatc tgacccgtga
      421 gatggttgcc tccggagacc gcctgacctt caacacccga ccgattgttg acaaacactc
      481 catcggaggt gttccgggga acaaaatcac gctccttgtg gtaccggtta ttgcggctgc
      541 gggtcttctc atccctaaga cgagttcccg tgctattacc ggtgcaggag gtacggctga
      601 tctgatggag gcgcttgccc cggttacttt caccgtgcag gagatccagc agatgacact
      661 gaaggccggc ggtgtgatcg tttggggtgg tgcaacaaac attgctcctg ctgatgatat
      721 gatcatcacg tatgagtatc cgctgaagat tgatgcacgg ggtcagatgc ttgcaagtat
      781 catggcgaaa aagatggcgg tgggctctga cacctgcgtg atcgatattc cggtaggtcc
      841 gggaacaaaa gtttccgatg aggccgaggg tagagtgctt gcaagcgagc tgattgagct
      901 tggccggaga cttggtattc gtgttgagtg tgcaattacg tacggcggtg ctccagttgg
      961 cagaaacatt ggtgtgaatc tggaggttgc cgaggctctc agaattcttg aaggcgaaag
     1021 cggaccaaac tcgctcatgc agaagagtgc cgctcttgcg ggcatggcac ttgagatgac
     1081 gggaaaaaca gcgtatggat acggagcaga agcagcgatg gatcttatcc ggagtggaaa
     1141 ggctctggca aagatgcggg atatcattga ggtacaagga ggggatccta agatcaagtc
     1201 atcggatctt gtgtttggtt cgaacgtgtt tgatgttccg gcaccaaacg acggatacgt
     1261 agtctccgtg aagaatcgtg atctgataaa tatcgcccga accgcaggat cacctgcgga
     1321 tcacggagca ggtctgcacc tgcataagaa accgggagag gcggtgaaga agggagagcc
     1381 gatcctgacg atttatgcag agcggggatg gcggcttcag cgtgcgattg aggaggcaag
     1441 agttagcatg ccggttgtgg tggagggtat gcttctggat cgtgtgccgc cgatccggcg
     1501 tgtggcataa ctattggtgt agatttccca cctaattttt tggtcattta cccggtatct
     1561 gctgatgatc gttgatgata atttgcttta tctgtggtta cctttgttat gaccacatca
     1621 gtgattattt tttgaaaaga gagctttcaa tttttttctc aaaaagagat tggtatctct
     1681 caggcattcc cctgaggatt tatcatctct tttttatttc acgctgctta gttgcggcgg
     1741 aggacgagca gtgctactgc accaagtcct gcgagtgcaa cgaatgc
//
LOCUS       DALUAJ010000120         3182 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_21776,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000120 DALUAJ010000000
VERSION     DALUAJ010000120.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21776"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1699)
                     /locus_tag="O0X37_02590"
     CDS             complement(<1..1699)
                     /locus_tag="O0X37_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61849.1"
                     /translation="MRVSEGNLTQQYYCDYLEILAEEILSASVTEEVRRGVHENIVFR
                     NLFLHRMGVDGGFHARVISAILHQPDDSRLLMDLLMASKQVDTDVLPVEEWLKQEYAS
                     AVDKQNSARMVAVEALLRSYISGTNQAAEIIASAGDSHPQSWWQLETRRITIAEVANP
                     DMYFGVGRRPFWHEFPLDRYAAVVAGVPDLLPHEIYDCVVCIAGAWFSSLTREEMITW
                     LNMPQPTLSEWETLCAPLLKCDTPDAALSTANWLYASGNNDPAMDLYANITLAFPGTP
                     AEISSFELIGTILRKLGDYDNAFEAYKNAFIASRGAGGYQIAIGLKNLCEIGEDLGED
                     MSDYYTRIAGIADMLPVSEKLRLYLDLAASCRRRHAYEEEYRYLEQVLDAEDEGDEDL
                     VAAAMSRLSEMNMSLSADGTPDTLLLAAKDEEAESSVSVTRGTAAYFGFDPASALMWY
                     ERSGSSHISSLQFAAAMAAGVDAEEYAKTPSQRAVVLAAEGSPVTEIVRELNVAVTDA
                     WRSGEDAVQVFEQIIPYLTPDQMSTVFVEVTARSTRDDERAVVCSAAAKALLSAGYVN
                     "
     gene            complement(1872..2891)
                     /gene="mtrH"
                     /locus_tag="O0X37_02595"
     CDS             complement(1872..2891)
                     /gene="mtrH"
                     /locus_tag="O0X37_02595"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit H"
                     /protein_id="HJK61850.1"
                     /translation="MFKFEKEQQVFDFNGTKIGGQPGEYPRVLGASIFYNKHECVLDD
                     HTGKIDKAKAEALWNRCQELTDETGNHFFLQIIAEHGEAFESYFQWFDSIDNKTAFLM
                     DSSAPEALVHATKYVTEVGLADRAIYNSINGSIQPENIQALAESDVDAAIVLAFNPGD
                     GSVAGREKVLSEGGVAGQAKGMLQIAEECGIKRPILDTAATPLGLGSAGSFREILACK
                     AIHGLPTGGAYHNMTVSWTWLKRWRKNIHEQYKGKDILAEQMFHHHYGGIEGVRQIAW
                     SSPDIGCNIMAMTLGADLIMFGPIENMEGIATAAAFSDIVLSEALTELGGEVQDHNGP
                     LYKLV"
     gene            complement(2906..>3182)
                     /gene="mtrG"
                     /locus_tag="O0X37_02600"
     CDS             complement(2906..>3182)
                     /gene="mtrG"
                     /locus_tag="O0X37_02600"
                     /EC_number="2.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030269 - tetrahydromethanopterin
                     S-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019386 - methanogenesis, from carbon
                     dioxide [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetrahydromethanopterin S-methyltransferase
                     subunit G"
                     /protein_id="HJK61851.1"
                     /translation="DPGAFGADPIVVQISDDEGGAAGGATVASANPQFLEIEKRLDAI
                     EKKIEFTDAEIAMRVGRKIGRDIGILYGLVAGCIAFIVILMLGLNFM"
BASE COUNT          836 a          854 c          718 g          774 t
ORIGIN      
        1 cattcacata tcctgcggaa agtaatgcct tcgctgccgc ggaacacaca acagcacgtt
       61 catcatcgcg ggtggaacgt gctgttacct cgacaaaaac agtactcatc tgatcgggag
      121 tgagatatgg aattatctgt tcaaacacct gtacggcatc ttcaccactc cgccacgcat
      181 cagttaccgc cacattcagt tcacgaacaa tctccgtcac cggtgaacct tctgctgcaa
      241 gaaccaccgc acgttgggaa ggggttttcg catactcctc cgcgtcaaca ccggcagcca
      301 ttgctgcagc gaactgtagc gatgagatat gtgaagaacc ggaccgctca taccacataa
      361 gtgcactggc aggatcaaat ccaaaataag cggcagttcc ccttgtaacc gaaacagaag
      421 actctgcctc ttcatctttt gctgcaagca aaagagtatc cggtgtacca tctgctgaaa
      481 gggacatatt catttctgac aaccgtgaca ttgcagcagc caccaaatcc tcatcaccct
      541 catcctctgc atcaagcacc tgctcaagat acctgtactc ctcctcatac gcatgacgtc
      601 tcctgcagga tgcagcaaga tccagataca gacgcaattt ttcagaaacg ggtaacatat
      661 ccgcaattcc agcaatccgc gtatagtaat ccgacatatc ttcaccgagg tcctcaccaa
      721 tctcacacag atttttcaaa ccaattgcaa tctggtaacc acctgcacca cgtgatgcaa
      781 tgaaagcgtt cttgtaggcc tcaaacgcat tatcataatc tcctaatttt cgcagaatag
      841 tacctatcag ttcaaatgac gatatctcag cgggggttcc ggggaaggca agagtaatat
      901 ttgcatagag atccattgcc ggatcattgt ttcctgaagc atacaaccag tttgcagtag
      961 aaagtgctgc atcgggggta tcacacttca aaagcggagc acagagagtt tcccactcag
     1021 acaatgtagg ttgcggcata ttcagccatg tgatcatttc ctcacgcgta agagaagaga
     1081 accatgcccc tgcaatgcag acaacacagt cataaatctc atggggaaga agatcaggta
     1141 cgcctgcaac aactgccgca taccggtcaa ggggaaattc atgccagaac ggacggcgcc
     1201 caacaccgaa gtacatgtca ggatttgcaa cctcagcaat tgtgatacgc cgtgtttcaa
     1261 gttgccacca actctgggga tgtgaatccc cggcagatgc aatgatctcg gctgcctgat
     1321 ttgttcccga aatataggat cgcagcagtg cctcaactgc taccatgcgt gcactgttct
     1381 gtttatcaac tgctgatgcg tactcctgct tcagccactc ttcgaccggt agcacatcgg
     1441 tatcaacctg ctttgatgcc ataagaagat ccatcagaag ccggctatca tctggttgat
     1501 gcaggattgc agagatcact cgtgcatgga aacctccatc tacacccatt cggtggagaa
     1561 acaaattgcg gaacacaata ttttcatgca cacctctccg cacctcctca gttacagacg
     1621 cagataatat ctcttctgca agaatctcca gataatcaca ataatattgt tgggtcagat
     1681 tcccctcgct gactcgcata cttatgtagt gttttgtacg tttaccctat gtatgccttc
     1741 tgatagatct aagaaagtga aaaaagaaca caaaaccgaa tcgggataac ccgttactgc
     1801 aggtgataac caggataaac ccgatttttt cccacactac agaaaaaaat tgattttatt
     1861 ggtcaggaag attagaccag cttgtacagc gggccgttgt gatcctgaac ctcgccgccg
     1921 agctcggtaa gagcctcgga cagaacgatg tcggagaagg ctgctgcggt tgcaatacct
     1981 tccatgttct caattggacc aaacatgatc agatctgcac caagggtcat tgccatgatg
     2041 ttacatccaa tatctgggga agaccaggca atctggcgaa cgccctcgat accgccatag
     2101 tggtggtgga acatctgttc tgcaaggatg tccttaccct tgtactgctc gtggatgttc
     2161 tttctccagc gtttcagcca ggtccaggac acagtcatgt tgtggtatgc accaccggtc
     2221 ggcagaccgt ggattgcctt gcatgcaagg atctcacgga acgaaccggc agagccaaga
     2281 ccgagcggag ttgctgcagt gtcgagaatt gggcgtttaa taccgcactc ttctgcaatc
     2341 tgaagcatac cctttgcctg acctgcaaca ccaccctcag aaagtacctt ctcacggcct
     2401 gcaacggaac cgtctcccgg gttgaatgca agaacaattg cagcgtcaac gtcagactcg
     2461 gcaagtgcct ggatgttctc cggctggatg gaaccattga tggagttgta gattgcacgg
     2521 tctgcaagac caacttctgt aacgtacttc gttgcgtgaa cgagtgcctc aggggcagat
     2581 gagtccatca ggaatgcagt cttgttgtcg atgctgtcga accactggaa gtaggactcg
     2641 aatgcctcgc cgtgttctgc aatgatctgg aggaagaagt ggtttccggt ttcgtccgtc
     2701 agctcctgac agcggttcca gagcgcttct gctttcgctt tgtcgatctt accggtgtgg
     2761 tcatcaagaa cacactcgtg cttgttgtag aagatggatg caccaagaac acgcgggtat
     2821 tctccgggct gtccaccaat ttttgttccg ttaaagtcaa agacctgttg ttccttttcg
     2881 aacttgaaca tattggcacc tcaatttaca taaagttcag ccctaacatc agaatcacaa
     2941 tgaatgcaat acatcctgct acaagaccgt agaggatacc gatgtcacga ccaatttttc
     3001 ttccgacacg catcgcgatt tctgcgtccg tgaactcaat cttcttctcg attgcgtcaa
     3061 gacgcttctc gatctcgagg aactgcgggt ttgcacttgc aaccgttgca cctccggcag
     3121 ctccgccttc gtcatcagag atctgaacaa cgattgggtc ggcgccgaat gccccgggat
     3181 cg
//
LOCUS       DALUAJ010000121         7008 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_22008,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000121 DALUAJ010000000
VERSION     DALUAJ010000121.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22008"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="O0X37_02605"
     CDS             complement(<1..984)
                     /locus_tag="O0X37_02605"
                     /inference="COORDINATES: protein motif:HMM:NF014073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HJK61852.1"
                     /translation="MNEYWTARVNILSRRYSGMLGLAAGCVAGVLLGTISGIIPGVHS
                     NTVAGFLAGASGPLLVLFGPEGLAAAIVATMVTHTFLDAIPSTFLGVPDPDTVLSVLP
                     AHRLCLAGHGEEAVRVSALGSVAGFVLCLPLFVIFMFLLPPMQGYIDWGIGLVILLAA
                     GLLIVFSRSPGWALAVFGVSGILGVFSLGYAHFSFGVFGVGEVLLPLLTGLFGVPVLL
                     SSIRSIAEIPPQKFTGLLIGRRETLWSGLRGAVAGAVVGWLPGFSSGTANALLAIRRD
                     GDFEKTDPREYLIATSAANTANAVLGIAALYAIGRMRSGAMVALASLELPSL"
     gene            983..1681
                     /locus_tag="O0X37_02610"
     CDS             983..1681
                     /locus_tag="O0X37_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HJK61853.1"
                     /translation="MLLAITRLAEKGAADAALCKQYGHECRVVSSLRSEIRSSTIQTF
                     VLAASDNEFDAIFFTSALPAQKIAPLLNPGITKTTRVIAIGPQTAKVLHESGIAAETL
                     PTYYSRDFVPYLGSWIKEKRIGIPRADVPNPDLINAIEEAGGMAFEYRCYGLEPTNEP
                     LNLDGVDAILFTSANSYKMAILPPLTSSILPMAIGEITAAAMRAGGLEPNVVGDGSLE
                     GTLKNLNTYLRSRE"
     gene            1678..2535
                     /locus_tag="O0X37_02615"
     CDS             1678..2535
                     /locus_tag="O0X37_02615"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided pseudouridylation complex
                     pseudouridine synthase subunit Cbf5"
                     /protein_id="HJK61854.1"
                     /translation="MSYTGVLLVDKPQGPSSHQVAAWVGSMLKSSVGHSGTLDPMVSG
                     VLVVMLGKAVRLAPLLLLHEKEYVACLRLHADVPRERVEEAVKQFTGRIYQRPPRRSA
                     VKRALRIRVIHRIELLDMQDRLVLIRVRCEAGTYIRSLCVHIGRILGCGSQMIELRRT
                     RSGGFSVEDTHTLHEIRDAVERGDEDLIRSMLLPPEKAITDIPKIVVTPKAADAVAHG
                     AVLAGVGVLQCDPFVQKQNVAIITEEGKLIALAEAVRDSAKFVCGEPGFVARSTRVFV
                     SPNDVNKKY"
     gene            2569..2652
                     /locus_tag="O0X37_02620"
     tRNA            2569..2652
                     /locus_tag="O0X37_02620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2601..2603,aa:Ser,seq:gga)
     gene            complement(2766..3695)
                     /locus_tag="O0X37_02625"
     CDS             complement(2766..3695)
                     /locus_tag="O0X37_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HJK61855.1"
                     /translation="MDYFIALNQILILFLLIGVGFFCRRVGILNETSVASLSKFLLHI
                     CIPAMIIYSMQIPFTSELLQNGELLILAAFGYYAVSLIVAWFAPILLRAKHEEYGVFR
                     FMLVFSNSMFMGFPVLSMIFGSEAIFYAAIFNIPFTVLAYSLGIWLLTVHGGGEGRPV
                     FQPKLLLNAAFLSTFVGLALFLTSTTIPDPINGSLELLDSVTTPLSLVVTGGFLAQLD
                     LSRIFGNVRQYFVAGIRLLILPALVYVIFSPFIEDPLILGVVVVTAGMPAATNTVMLA
                     SEHRVHPDIAAQGVFITTLVSVFTLPLLAMFLS"
     gene            3777..4316
                     /locus_tag="O0X37_02630"
     CDS             3777..4316
                     /locus_tag="O0X37_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61856.1"
                     /translation="MDEIVEQGFDRLQTLISETKQKQDDAYRELCEQSALLLSRMIEA
                     VAPIAGEIGSEFLLKAKQDTKGELYDQKYYDEKMIILGKTDSPMEYRPDDPKKKVTQQ
                     FCVLSEKGVLYELMFSNDGFVVDTFASPLTPEDALVFYGYDIMYMLYSAMRDYYLAEE
                     EILASLDIALGYIQKKTQN"
     gene            complement(4351..4424)
                     /locus_tag="O0X37_02635"
     tRNA            complement(4351..4424)
                     /locus_tag="O0X37_02635"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4388..4390),aa:Ile,seq:gat)
     gene            4552..6339
                     /gene="iorA"
                     /locus_tag="O0X37_02640"
     CDS             4552..6339
                     /gene="iorA"
                     /locus_tag="O0X37_02640"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HJK61857.1"
                     /translation="MAEEKNKQYLLGNDAIAHGCLEAGVDFVSGYPGTPSSEVIDTLR
                     RVKDPWYYLEWSVNEKVAFENAIGACWCGLRSIVTMKHVGLNVAADPLMTSSYTGVKG
                     GFVILSADDPFAHSSQNEQDSRMYAKFAQIPCLDPKDVQQAHDMITAAWDLSEKFSLP
                     VLFRPTTRICHSKSDVALGDPSPSPRKGEFVHDPKQYVVIPAHTRPLHAVLTKKQADV
                     AKYLVEAGFNSADIRGTRAVIAGGISVSYVEEILPEDVSLIRIGAYPIDQTWLADVVA
                     KHTEVLVVEELMPVIEEAVRQAACTTVVHGKLDGYLPHEGEFSTALVAKALVKSGFLS
                     ENPFPPEPAQEAVAVRPPILCPGCLHRSVFYAMKKVFRDGVFPSDIGCYTLGLQMGAV
                     DTTICMGASVTVGSGIAHACPGTDVVSTIGDSTFLHTGVNGLINAVYNNANQTIIILD
                     NRITAMTGHQPNPNSGVTATGVESPKISLEELARACGVSFVESVDPYDLTDLLETLKA
                     AKERPGVKVIIAKQPCVIMNKRNGVKRSRYVVDADACIKCGACIRYGCPALETREDGT
                     SVTTALCTGCGVCADICPAGAIHRGGAKQ"
     gene            6336..6920
                     /locus_tag="O0X37_02645"
     CDS             6336..6920
                     /locus_tag="O0X37_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HJK61858.1"
                     /translation="MKKSYDVLIVGIGGQGTILASNVLGDACVIEGRHVRGAETHGMS
                     QRGGSVETHIRIDGKFGSLIAPGSADLLIAFDVLEALRYRHFLKENGVMVVNREMVVP
                     TSVFSAKLPVPQMEDVVAQLKSGKATVLLVDATEIATKAGSPLAANIVLLGAASHQLP
                     LSVSSLEEAVRRNVPQKTIALNEQAFAMGREVRL"
BASE COUNT         1832 a         1801 c         1818 g         1557 t
ORIGIN      
        1 cagcgaggga agctccagtg aagctaaggc caccattgcc ccgctccgca tccgcccgat
       61 tgcatacaaa gctgcaattc ccagaactgc atttgcagtg ttggccgcac ttgttgcaat
      121 caggtactcg cgtgggtccg tcttttcaaa atcaccatcg cgtctgattg caagaagtgc
      181 atttgcagtt ccgcttgaaa atccgggaag ccatccgaca acagcacccg caaccgcacc
      241 acgcagaccg ctccagagag tctcacgacg tccaatcaac aggccggtaa acttttgagg
      301 aggaatctcc gcgatcgaac gtatcgacga caacagaaca ggaacaccaa acagccccgt
      361 cagcagaggc agcaatacct ctccaacccc aaacactccg aatgaaaaat gtgcgtaccc
      421 gagcgaaaac acaccaagaa ttcccgaaac cccaaacacc gcaagagccc atccgggaga
      481 tcgtgagaac acgatcaaaa gacctgctgc aagcaaaatg acaaggccga tgccccagtc
      541 aatgtatccc tgcatcggcg ggagaaggaa cataaatatc acaaacagcg ggagacacaa
      601 tacaaatcct gcaacactcc ccagagccga cacccggact gcctcttccc catggcccgc
      661 aaggcacaac cgatgggcag ggagaaccga cagaacagtg tcaggatccg gcacaccgag
      721 aaatgttgac ggaatcgcat cgaggaacgt atgcgtaacc atcgttgcaa caatcgcggc
      781 tgcaagcccc tccgggccga acaaaaccag aagaggaccg gaagcacctg ccagaaatcc
      841 cgcgaccgta ttggagtgta cacccggaat aattcctgaa attgttccaa gaagaacacc
      901 tgccacacat cccgcagcaa ggccaagcat acctgaatat cgacgggaca gaatattaac
      961 tctggccgtc caatactcat tcatgttact tgcaatcacc cgtcttgccg aaaaaggtgc
     1021 ggcagatgcg gccctctgca aacaatatgg acacgaatgc cgtgtcgtct catcacttcg
     1081 ttctgaaatc cgcagcagta cgattcagac ctttgtcctt gcggcatccg acaacgaatt
     1141 tgatgcaata tttttcacaa gcgctctccc cgcacaaaaa attgcgccgc tcctcaaccc
     1201 cggtatcacc aaaaccacgc gggtaattgc cattggcccg cagaccgcaa aagttctgca
     1261 tgaatccggc attgccgcag aaacacttcc aacctattac tcacgtgact tcgtcccgta
     1321 ccttggcagc tggatcaaag aaaaacgcat cgggattcct cgtgcggatg ttccaaatcc
     1381 tgatctgatc aacgcgattg aggaggcagg cgggatggcg tttgagtacc ggtgttacgg
     1441 acttgaacca accaacgagc cgctgaacct cgacggcgtt gatgcgatcc ttttcaccag
     1501 tgcaaactcg tacaaaatgg ctatccttcc tcctctaacc tcatcgattc tcccgatggc
     1561 tatcggagag ataacggcgg ctgcaatgcg tgccggaggt ctggaaccga acgttgtcgg
     1621 agatggctcc cttgagggga ctctcaaaaa tctgaacact tatctgcgga gtcgcgaatg
     1681 agttatacgg gcgttctgct ggtggataaa ccacaagggc cgagcagcca tcaggttgcg
     1741 gcatgggttg gcagtatgct caaaagtagt gtgggacatt cggggaccct tgacccgatg
     1801 gtctcaggtg tgctggtggt tatgctcgga aaagccgtgc ggcttgcacc cttactgctt
     1861 ctgcatgaga aagagtacgt ggcatgtctc cgtctccatg ccgatgttcc ccgcgaacgt
     1921 gttgaggaag ctgtaaagca gttcacgggc cgcatttatc agcgcccgcc gcggcgttcg
     1981 gcggtgaagc gggcactgcg catccgggta attcaccgaa tcgagcttct ggatatgcag
     2041 gatcggctgg tcctgatccg ggttcgctgt gaggcgggaa cctacatacg atcgttgtgc
     2101 gtccacatag gcaggattct tgggtgtggt tcgcagatga tcgagttgcg gcgtacacgt
     2161 tccggcggat tttcggtgga ggacacacac actctgcatg agattcgtga cgccgtggag
     2221 cggggtgatg aagatctgat tcgttccatg ctgctccccc ctgagaaggc catcacagac
     2281 attcccaaga ttgttgtcac gcccaaagca gcggatgccg ttgcacacgg cgccgttctc
     2341 gcgggtgtag gcgtactaca gtgtgatccg tttgtacaga aacaaaatgt tgcaatcatt
     2401 accgaagagg gaaaactgat tgcgcttgca gaggcagtcc gtgactcggc aaagtttgtc
     2461 tgcggagagc cgggatttgt ggcacgatct acccgggttt ttgtctcgcc caatgatgta
     2521 aacaagaaat attaataaac acagacaacg cacgtaatat gctagtttgc tgaggtagtc
     2581 tagcggtagg gcgcgggcct ggaaagcccg tgaggcgaaa gtctctcggg agttcaaatc
     2641 tccccctcag cgtttttgtt tttcttgtgt aatttctgta tagagttgga tccgttttcc
     2701 gataaatatc gtactgaaca ggagttttgt tctctaaaaa agtggaaatg aattgcggct
     2761 gtgtttcacg acaggaacat cgcaagaagc ggcagtgtaa atacactcac cagcgtggtg
     2821 ataaacacgc cctgtgccgc aatatccgga tgcacacgat gttctgatgc aagcatcacg
     2881 gtgtttgttg ccgccggcat acctgctgtc accaccacca cacccaggat cagcggatct
     2941 tcaatgaacg gcgagaagat cacatatacc agggccggca gaatcagcag ccggatcccc
     3001 gcgacaaaat actgccgtac atttccaaag atccgcgaaa gatccagctg tgcaagaaaa
     3061 cctcccgtaa caaccaggga cagcggtgtc gtcacgctat ccagcagctc cagagatccg
     3121 ttaatcggat ccgggatcgt tgttgatgtc agaaacagtg caagacccac gaacgttgag
     3181 aggaatgccg catttaacag cagcttcggc tgaaacactg ggcgtccttc tcctcctccg
     3241 tgaacggtca gcagccagat gcccagtgag tatgccagaa ccgtaaaggg aatgttaaag
     3301 atcgctgcat aaaatatcgc ctctgatcca aagatcatcg acagaaccgg gaatcccata
     3361 aacatcgagt tcgagaacac cagcatgaac cggaataccc cgtactcctc atgttttgca
     3421 cgcaggagaa tgggtgcaaa ccatgcaaca atcagtgaaa ccgcataata cccaaatgct
     3481 gcaaggatca acagctcacc attctgcaag agttctgatg taaacggtat ctgcatcgag
     3541 tagatgatca ttgccggaat acagatatgc agcaaaaact tcgaaaggga tgcgactgac
     3601 gtctcattca aaattccaac acgcctgcag aaaaatccca ccccgataag gaggaacaga
     3661 attaaaattt ggtttaaggc gataaaataa tccacagcct atggcctcct ttactattat
     3721 ttacaatata aaaccgaacc gatagttaaa ctatcggacc cgtccatacc tatggtatgg
     3781 acgaaatcgt cgaacaggga tttgaccgtc tccagacact cattagcgag acaaaacaga
     3841 agcaggatga cgcataccgt gaactctgtg aacagagcgc attactcctc tcccggatga
     3901 ttgaggctgt tgcaccgatc gcaggtgaga ttggatcaga gttcctgctc aaagcaaaac
     3961 aggataccaa aggggaactc tacgatcaga aatactacga cgaaaagatg atcattcttg
     4021 gaaaaaccga ctccccgatg gagtaccggc cggatgatcc gaagaaaaaa gttacacagc
     4081 agttctgcgt cctctctgaa aaaggagttt tatatgaact catgttctcc aatgacggat
     4141 tcgttgttga cacctttgca tcaccactta ctccggaaga tgcacttgtg ttctacggct
     4201 atgatattat gtacatgctc tactccgcaa tgagagacta ctatcttgca gaagaggaaa
     4261 tacttgcatc cctggacatt gctctggggt acattcagaa aaagacccag aactaacttt
     4321 ttttagttac aaaaaatcac agaaaaacag tgggcccgat gagattcgaa ctcatgacct
     4381 cccggttatc agccgggcgc accacctagc tatgctacgg gccccttttg ggtgttaccc
     4441 taatacatag gatatagaaa tataaataga ttgtgaattg tgagttgaga cagacttaac
     4501 tctttatcct gctaaacgta atggaagaag tatatcgtgg caggatagag aatggcagaa
     4561 gagaaaaaca aacaatatct cctgggaaac gatgcaattg ctcacggttg tctggaagcc
     4621 ggagtggact ttgtatccgg ttacccggga acaccttcgt ccgaggtcat tgacacgctc
     4681 cgccgggtta aggatccctg gtactatctt gagtggtcgg tcaacgaaaa agtcgcattc
     4741 gaaaatgcaa tcggtgcatg ttggtgcgga ctgcgctcca ttgtgacgat gaaacatgtt
     4801 ggtctgaacg tcgcagcaga tccgctgatg acctcctcct acaccggggt aaaaggagga
     4861 tttgtcattc tctccgcgga cgatcccttt gcccacagct cacagaacga acaggactcg
     4921 cggatgtatg caaagtttgc ccagatcccc tgtcttgatc caaaggatgt ccagcaggct
     4981 catgatatga tcacagctgc ctgggatctc tcggagaagt tttctctgcc ggtactgttc
     5041 cggccgacaa cccgtatctg ccactcgaag agtgatgtgg cgctgggtga tccgtcgcca
     5101 tccccaagaa agggcgagtt tgttcatgac ccgaaacagt acgtagtcat ccctgcccat
     5161 acccgtcccc tccatgcagt gcttactaaa aagcaagccg atgttgcgaa gtatctggtt
     5221 gaggccgggt ttaactccgc agatatccgg ggaacacgtg cggtgatcgc cggaggaatc
     5281 tcagtgtcct atgtggaaga gattcttccg gaggatgtat cactcattcg tatcggtgca
     5341 tatccgatcg atcagacctg gcttgcagat gtcgtggcaa agcacacgga ggttctggtg
     5401 gtggaagagc tgatgccggt gatcgaagag gccgtacgtc aggcggcatg cacaaccgta
     5461 gtgcatggaa aacttgacgg ttacctgccg catgagggtg agttctccac ggcccttgtt
     5521 gcaaaggcac tggtgaagtc cggtttcctt tctgaaaatc cgttcccacc cgaaccggca
     5581 caggaggcag ttgcagtgcg gccgccgatt ctctgcccgg gatgcttgca ccgttccgtg
     5641 ttttatgcaa tgaaaaaggt gttccgtgac ggcgtattcc ccagtgacat cggctgttac
     5701 acccttggtc tgcagatggg ggcagttgac accacgatct gtatgggagc atcggttacg
     5761 gtaggttcgg gtattgccca tgcctgtccg ggaacggacg ttgtttcaac aatcggtgac
     5821 tcgacgtttt tacataccgg ggtaaacgga ttgatcaacg cagtgtacaa caatgcgaac
     5881 cagacgatca ttattctgga caaccgtatt acagcaatga cgggccacca gccaaacccg
     5941 aactctggtg taacagcaac aggtgtggag tctccgaaga tatctctgga agagcttgca
     6001 cgtgcctgcg gtgtttcgtt tgtggagtct gttgacccgt acgatctgac ggatcttctg
     6061 gaaacactaa aggctgcaaa agagagaccg ggtgtgaagg tcatcatcgc aaaacagccg
     6121 tgtgtgatca tgaacaaacg caacggtgta aagcgcagcc ggtatgtagt ggatgcagac
     6181 gcctgcatta agtgcggagc gtgtatccgg tacggctgcc ctgctctgga aacccgtgag
     6241 gacggaacat cggtaacaac tgcgttgtgt accggatgcg gtgtgtgcgc agacatttgt
     6301 cctgcgggtg cgattcaccg gggaggtgca aagcaatgaa gaagagttac gatgttctga
     6361 ttgttggtat tggcggtcag gggacgattc tcgcatcgaa tgtactgggt gatgcgtgtg
     6421 tgatcgaagg acggcatgtc cgcggtgcag agacgcacgg gatgtcacag cgcggcggat
     6481 cggtggagac gcatatccgg attgatggaa agttcggttc actgattgca ccgggttctg
     6541 cggatcttct gattgcgttt gatgttcttg aggcgctccg gtaccggcat ttcctcaaag
     6601 agaacggcgt gatggtggtg aaccgtgaga tggttgtacc tacctctgtg ttctcggcaa
     6661 aactgccggt tccgcaaatg gaggatgtcg tggcccagct gaagtccggc aaggcaacgg
     6721 tgctcttggt ggatgcaact gagattgcaa caaaggcagg cagccctctt gcagcaaata
     6781 ttgtcctcct aggtgctgca tcccaccagc ttccgctctc ggtctcctcc cttgaagaag
     6841 cggtccgcag gaatgttccg cagaaaacaa ttgccctgaa tgaacaggca tttgcaatgg
     6901 gccgagaggt ccgtctctaa cctttttttt gatttttgtt tttgggaaac cttcgtggta
     6961 cactaacgta cagattgtca ttttggaaca ttgcaaaata tccaatac
//
LOCUS       DALUAJ010000122         1804 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_22021,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000122 DALUAJ010000000
VERSION     DALUAJ010000122.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22021"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="O0X37_02650"
     CDS             complement(<1..409)
                     /locus_tag="O0X37_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61859.1"
                     /translation="MTNESSPTTPTLDEAMQLFNAGQFTDVISLVSGTSDPALLLLAA
                     RSYAETGQYDTAGYLLRDLIQVMPGSSYLHSYLADVMEKTGDEHAVSEYAAALILDPE
                     NRPALRSYARLLLDKNDLRGAIPSLRTLVRFGSD"
     gene            complement(414..926)
                     /locus_tag="O0X37_02655"
     CDS             complement(414..926)
                     /locus_tag="O0X37_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HJK61860.1"
                     /translation="MKILFAIAPDRFLDQEYTIPKAAFEEKGIECVTASTIRGTCYGM
                     HGEIVESDLSFDEVNPADYDGIIIVGGIGCQDYLWRSEKLIDITNAMGTAGKITAGIC
                     LAPVVIAEAGLLAGKNATTLDTPASRRVMELDKAVLVNEPVVVSGNIITARMPQDAKA
                     FADTILQHLA"
     gene            complement(1000..>1804)
                     /locus_tag="O0X37_02660"
     CDS             complement(1000..>1804)
                     /locus_tag="O0X37_02660"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK61861.1"
                     /translation="FDVRGTMLYAVAMFCTMYGLSTLPDLLSAVLTVIGIILMAAFVW
                     YELRIPSPVLHVKLFFTNQRFARSSYAALLNYGCTYGSVFFVSLYLQSVGQLTAAEAG
                     MILFFQPLIQAIMTPIAGRFSDRIDPRYIVTIGMILSGIGVLLLAGLDTGTDLHYIAV
                     TQVFIGLGSALFSAPNTNAIMGSVPAEEYSTASGIVAVMRQAGMIISMAVCMSAISVY
                     VGGTDMLGPEMYQEFLQALKVSMIFCAVLAFVGVLFSWFRGKGTVPDAS"
BASE COUNT          450 a          453 c          490 g          411 t
ORIGIN      
        1 agtcagaacc gaaccgcacc aacgttcgca gtgagggtat tgcaccgcgg agatcatttt
       61 tgtcaagaag cagacgggca tagctcctga gagcaggccg gttttccggg tcgagaatca
      121 gtgcggctgc atactcggat acagcgtgct cgtcgccggt tttttccatg acgtcggcaa
      181 gatagctgtg caggtacgat gatcccggca ttacttgtat gagatcccgg agaaggtatc
      241 ctgcggtatc gtactgaccg gtttctgcat aactccgggc agcgagaaga aggagagcgg
      301 gatcgctggt gccggagacg aggctgatga cgtcggtgaa ctgtccggcg ttaaagagct
      361 gcatcgcctc atcaagtgtg ggagtggttg gggaagattc gtttgtcatt gtgtcaggcg
      421 aggtgttgaa ggatggtatc ggcgaaggct ttggcgtcct gcggcatgcg tgcggtgatg
      481 atgtttccgg agacgaccac cggttcatta acgagtactg ctttgtcgag ttccattaca
      541 cggcgtgatg caggcgtgtc aagcgtggtt gcgttctttc ctgcaagaag tccggcctcg
      601 gcgatgacga ccggtgcaag gcagatgcct gcggttattt ttccggcagt acccatggca
      661 ttggtaatgt caatgagttt ctctgatctc cagaggtaat cctgacagcc aatgccgccc
      721 acgatgataa tgccgtcgta atcagcgggg ttgacctcat caaaggagag atcggactca
      781 acaatctcac catgcatgcc atagcacgtt ccgcggatgg tggaggctgt tacgcactca
      841 attcctttct cttcgaaagc tgctttgggt atggtatatt cctgatctaa gaaccggtcc
      901 ggagctattg cgaaaagaat cttcatatgt atttactatg gttttcccga gggattaagg
      961 gtttggtact gaataggcag tactatacat cccaaatcct tagcttgcat caggcacagt
     1021 acctttacca cggaaccagg aaaagagtac tcccacaaat gcaagtaccg cacagaagat
     1081 catcgaaact ttcagtgcct gtaagaactc ctgatacatt tctggtccga gcatatccgt
     1141 cccgcccacg tacaccgaaa tcgctgacat acagaccgcc atcgagatga tcattcctgc
     1201 ctgccgcatc acagcaacaa ttcctgatgc tgtactgtac tcctctgccg gtactgatcc
     1261 cataatcgcg tttgtgtttg gagcggagaa caacgctgat ccaagaccaa taaacacctg
     1321 ggtgactgca atgtaatgca gatcggttcc cgtgtccagt cctgcaagca ggagaacgcc
     1381 gattccggag agtatcatac cgatggtgac aatgtagcgg gggtcgatcc tgtcggagaa
     1441 tctccctgca atcggtgtca tgatcgcctg aataagcggc tggaagaaga ggatcattcc
     1501 tgcctctgct gcggtaagtt gtccgaccga ctgcaggtac agactcacga agaagaccga
     1561 accgtaggta cagccgtagt tcagcagggc tgcatacgag gatcgggcaa accgctggtt
     1621 cgtaaagaac agtttcacat gcagcacagg cgacggaatc cgcagttcat accagacaaa
     1681 tgctgccatc aaaataatac cgatgacggt cagcacagcc gagagcagat ccggcagtgt
     1741 ggaaagacca tacatcgtac aaaacatcgc aaccgcatac aacatcgttc cgcgaacatc
     1801 gaac
//
LOCUS       DALUAJ010000123         1921 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_22231,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000123 DALUAJ010000000
VERSION     DALUAJ010000123.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22231"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="O0X37_02665"
     CDS             complement(<1..459)
                     /locus_tag="O0X37_02665"
                     /inference="COORDINATES: protein motif:HMM:NF021370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2070 family protein"
                     /protein_id="HJK61862.1"
                     /translation="MATEAKTENLSKYLFSAPVWWQSLIIIIVLGLLVDGAVYVLSGG
                     TGTMMYGVAFAAAAVAAMLLTKPLVDLLGTEKLTWNRSALVALASMIFSLLWMAIGFA
                     LNAALAYVLGLGFILAIRLMVLTAICDYRIGRMFLPAAVQTVAGIIFGVYL"
     gene            complement(468..1124)
                     /locus_tag="O0X37_02670"
     CDS             complement(468..1124)
                     /locus_tag="O0X37_02670"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR03679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HJK61863.1"
                     /translation="MTWAALTSGGKDSILALQKALDAGMDVSYMVTVVPENPDSYMFH
                     SANLAAVPVIAERCGMTYVGIPTLGEKEDELQDLEKGLAALPIEGVIVGAIESEYQRS
                     RVAALCDRLGLVMFAPLWHMDPLKLLYEVAERMDVRIVVTAADGLGENVLGKRIDADL
                     IDLLCRIAAKRRIHIAGEGGEYESLTLDAPCFSSPIETEGWTMTFSCGRGVAAIEKFW
                     "
     gene            complement(1121..>1921)
                     /locus_tag="O0X37_02675"
     CDS             complement(1121..>1921)
                     /locus_tag="O0X37_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HJK61864.1"
                     /translation="DRQNYYGGGAANIAVGIAKLGGTAELIAAVGRDYPGSAYEHHLK
                     EMGVTTRLFRTDKENCSTAFMVNNAAGDQITYFEWGASALFVTADAPSLSFAHMATGD
                     ADFNVKVAQQAEFATFDPGQDVKYYEASHLREIFENIDMLICNNFEAEIMCGKLGWTE
                     KELIASVPVAILTKGKNGSVLYQNGDVVEIPACPVTMVDPTGAGDAYRAGLLTAYRRG
                     YALPVCCKIGAVTSSFAVEKVGTQTNLADWEQMSERYAEQFGTLKKSE"
BASE COUNT          467 a          574 c          441 g          439 t
ORIGIN      
        1 cagatacacc ccgaagatta tgcccgcaac cgtctgtacc gctgcgggaa gaaacatccg
       61 tccaattctg taatcacaga tggcagtaag taccatcagc cggatggcaa gaataaatcc
      121 gaggccgagt acataggcaa gtgctgcatt cagtgcaaac ccaattgcca tccagagcag
      181 ggaaaagatc atggatgcaa gtgccaccag tgcagaccgg ttccaggtaa gtttttctgt
      241 accgagcagg tccacaagcg gttttgtcag aagcattgca gcaaccgctg ccgcagcaaa
      301 tgcaactccg tacatcatcg ttccggttcc gccggacaga acgtagacag caccgtccac
      361 aaggaggcca agcacgatta taataatcag tgactgccac cagaccggtg ccgaaaataa
      421 gtactttgaa agattctctg tttttgcttc agtagccatg acacctatca ccagaacttt
      481 tcaattgcag caactcctct gccgcaggaa aacgtcatcg tccacccctc ggtctcaatc
      541 ggagaggaaa aacacggggc gtccagggtt agcgactcgt actccccgcc ctcaccggcg
      601 atatggatcc gccgttttgc tgcaatgcgg cacaacagat ctattagatc cgcatcaatc
      661 cgttttccaa gtacattctc gccaagcccg tcagcagccg tcaccacgat ccgcacatcc
      721 atccgttctg caacttcata cagaagcttt agcggatcca tgtgccacaa cggcgcaaac
      781 atgacaagtc ccaacctgtc acacagtgca gcaaccctac tgcgctgata ctcggactca
      841 atcgctccca caattacacc ctcgatcggc agtgcggcaa gacccttttc cagatcctgg
      901 agctcgtcct ccttctctcc caacgtagga attcctacat aggtcatccc gcaccgttct
      961 gcaatcaccg gaacagcggc caagtttgcc gagtgaaaca tgtaggagtc gggattctcc
     1021 ggaaccaccg tcaccatgta ggagacatcc atccctgcat caagtgcttt ttgcagggcg
     1081 agaatggaat ccttaccccc ggatgtcagt gcagcccagg tcattcactc ttcttcaggg
     1141 tgccgaactg ctcggcatac cgttcgctca tctgttccca gtcggcaaga tttgtctggg
     1201 taccaacttt ttccaccgca aaggacgacg tcactgcccc gatcttacaa cagacaggca
     1261 gtgcgtatcc gcgccggtat gcggtaagga gacctgcacg gtatgcgtcc cccgcacctg
     1321 tcggatccac catcgtcacc ggacatgccg ggatttccac aacatcaccg ttttgataca
     1381 gaacacttcc gttctttccc tttgtgagaa ttgcaaccgg gacggatgca atcaattcct
     1441 tctccgtcca gccgagtttg ccgcacataa tctcggcctc aaagttgttg cagatcagca
     1501 tgtcaatgtt ttcaaagatc tctcgcagat gggatgcttc atagtatttt acatcctggc
     1561 cgggatcgaa ggtggcaaac tccgcctgct gggcaacttt cacgttgaag tctgcatcac
     1621 cggttgccat gtgtgcaaag gaaagagatg gagcatcagc tgttacaaaa agtgcacttg
     1681 caccccactc aaaataggtt atctgatccc ctgcggcatt gttcaccatg aacgcggttg
     1741 aacagttttc tttgtcggtg cgaaagaggc gggttgtaac gcccatctct ttgagatgat
     1801 gttcataggc acttcccggg taatcccttc caaccgctgc aatcagctcg gcggttcctc
     1861 cgagttttgc gatacccaca gcgatgtttg ccgctcctcc gccgtagtag ttctgccggt
     1921 c
//
LOCUS       DALUAJ010000124         2832 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_22281,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000124 DALUAJ010000000
VERSION     DALUAJ010000124.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22281"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="O0X37_02680"
     CDS             <1..267
                     /locus_tag="O0X37_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HJK61865.1"
                     /translation="ILLDYNIIKQLVEVYDHQVILNKDDPMVKALSPFQLPVLTDGDP
                     TSELLAEDICERLNRFCVEAGVDARVTKIRVWESDGCYAEVDAA"
     gene            264..881
                     /locus_tag="O0X37_02685"
     CDS             264..881
                     /locus_tag="O0X37_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJK61866.1"
                     /translation="MRVTETFVSLQGEGLRQGMPCMFVRFAGCNLHCAWCDTRYSLND
                     SQGVEMTPEEIMQAVLESGMRYVCITGGEPLLQKEDLVPLLAAMHEADVLVDIETNGT
                     VPFDDVQEFASVCMDVKCPSSGEMSDLSLLDQLRPTDSVKFVIGDEADYLYMVEVLAA
                     HPHLSAPVCITPVFGTDVQWLIETIISEKLPVRFQLQLHKAVNIP"
     gene            878..1549
                     /gene="queC"
                     /locus_tag="O0X37_02690"
     CDS             878..1549
                     /gene="queC"
                     /locus_tag="O0X37_02690"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HJK61867.1"
                     /translation="MKKAVCLLSGGMDSTTLAYVAKDMGYEILALHMNYGQRTERKER
                     ECAKKIANRLNAVDFVEISLDYFTKFGASSLTDMRIPVEEGTVGKADHPNTYVPFRNA
                     NLIAIATSYCEARDGDAVFIGVQSGDHIGYPDCTPQFIDAMQKVISIGTQTDKEIRLL
                     TPFVSMTKTDILREGLRLGVPYEDTWSCYDTEETSCGVCSSCLSRLKAFSDLGVEDPI
                     PYARR"
     gene            complement(1534..1914)
                     /locus_tag="O0X37_02695"
     CDS             complement(1534..1914)
                     /locus_tag="O0X37_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61868.1"
                     /translation="MTAEILTTEQILFAFICWICGGIFLIVSIAAYRSKRAVSFYAQM
                     PVAENRITNIPAYNRENAALWAAYSIPYFLIGFVGTTADLSISGILLVIVCVGSIPAL
                     ILAYQRIYRKYSGEPSVKKDQRRA"
     gene            2025..2525
                     /locus_tag="O0X37_02700"
     CDS             2025..2525
                     /locus_tag="O0X37_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HJK61869.1"
                     /translation="MPILYDTPRLKIETKPVHLPNGRDREYLFVQPVSAVCILPTDAE
                     SVYLIRQYRAVIDSYIYEVPAGGMESDDADPITAARRELAEEARFSAGELIPRGFVYS
                     SPGFCTEKLWLFEARDLSPCEEFARDEDEIIEVVRVAKSEAVAMVMDGRICDGKTIAL
                     LMRCLM"
     gene            complement(2552..>2832)
                     /locus_tag="O0X37_02705"
     CDS             complement(2552..>2832)
                     /locus_tag="O0X37_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61870.1"
                     /translation="IESRTLMETLNASHIAALKDDLLPRVKAAGPEQLIGLQREFLIL
                     RMNKLMVQEFFHRFPVYAIVPNFLLIADPEAQEVLDSTGDEKSIKDLL"
BASE COUNT          572 a          628 c          895 g          737 t
ORIGIN      
        1 atccttcttg actataatat catcaagcag ttggtggagg tgtatgatca tcaggtgatc
       61 ttaaacaagg atgatccgat ggtaaaagcc ctctctccgt tccagctgcc ggttttgacg
      121 gacggcgatc cgaccagtga gctgcttgca gaggatattt gcgagcgact gaaccggttc
      181 tgcgtcgagg cgggcgttga tgcccgggtg acaaagatac gggtctggga gtcggacggc
      241 tgctatgcag aggtggatgc agcgtgagag tcaccgagac gtttgtgagc ttgcagggtg
      301 agggactgcg gcaggggatg ccgtgtatgt ttgtgcggtt tgcagggtgc aatctccact
      361 gtgcctggtg tgatacccga tactcgctga acgattcaca gggtgttgag atgacgcccg
      421 aggagatcat gcaggcggta cttgagtctg ggatgcggta tgtctgtatt acgggaggag
      481 agccgctgct tcagaaggag gatcttgtac cgctgcttgc ggcgatgcat gaggcggatg
      541 tcctggtgga tattgagacg aatggaacgg ttccgtttga tgatgtgcag gagtttgctt
      601 ctgtctgtat ggatgtgaag tgtccgtcgt ccggggagat gagtgatctg tctctgctgg
      661 atcagctcag gcccacggac tcggtgaagt ttgtgattgg ggatgaggcg gactatctgt
      721 acatggtgga ggttcttgca gcccatccac atctttcggc gccggtctgt attacgccgg
      781 tgttcggaac ggatgtgcag tggctgatcg agacgatcat ttcagaaaaa cttccggtca
      841 gattccagct gcagctgcac aaggcggtga atattccatg aagaaagccg tgtgtctgct
      901 ttcgggcggg atggattcga cgactctcgc atacgttgca aaggatatgg gctatgagat
      961 tttggccctg cacatgaact acggtcagcg gacagagcga aaggagcggg agtgtgcaaa
     1021 aaaaattgca aaccgtctga atgcggtgga ctttgtggag atctccctgg attattttac
     1081 gaagtttggt gcaagcagtc tgacggatat gcgtattccg gtggaggagg gaaccgtcgg
     1141 gaaagcggat catccgaaca cctatgtgcc gttccggaac gcaaatctga ttgcgatcgc
     1201 aacgtcgtac tgtgaggcac gcgacggcga tgcggtgttt atcggggttc agtccggtga
     1261 tcacatcggg tatcctgact gtacgccgca gtttattgat gcgatgcaga aggtgatctc
     1321 catcgggacg cagactgaca aggagatccg gcttctgacg ccgtttgtgt cgatgacaaa
     1381 gacggacatc ctgcgtgagg gtctgcggct tggcgttccg tacgaggata cctggtcgtg
     1441 ctatgataca gaagagacct cctgcggtgt atgcagttcg tgtttgtcac ggctgaaggc
     1501 gttttccgac ctgggcgttg aggatccgat tccctatgcc cgccgctgat ctttttttac
     1561 ggatggttcg ccggagtatt tccggtatat tctctggtag gcaaggatga gtgccgggat
     1621 acttccgaca cagacgatca caaggagtat tccgctgatt gagaggtcgg cagtggtccc
     1681 aacaaatccg atgaggaagt acgggatgct gtatgccgcc cagagggccg cattttcccg
     1741 gttgtacgcg ggtatgttgg tgatgcggtt ttctgcaacc ggcatctgtg cgtaaaagga
     1801 tactgcccgt ttgctgcggt aggcagcgat ggacacgatc aaaaagattc ctccgcagat
     1861 ccagcagatg aaggcaaata ggatctgttc ggttgtgagg atttctgctg tcatctgtgc
     1921 ctcgcggacg ggtttctggt tatgtgtatg gcgggtctgc tactttattc tgtctgaggg
     1981 tttttatttc tccacagcca atggagtttc agtgagcatt ttccatgccg attctgtatg
     2041 ataccccgcg tctgaagatc gaaacgaaac cggtacatct cccgaacggc cgcgaccggg
     2101 agtatctctt tgtccagccg gtatctgcgg tgtgtattct gccgacggat gccgagtcgg
     2161 tgtatctgat tcgtcagtac cgggcggtga tcgattcgta catttacgag gttccggcag
     2221 gcgggatgga gtcggatgat gctgatccga tcactgcagc ccggcgcgag cttgcggagg
     2281 aagctcgttt ttctgccggt gaactgattc ctcggggatt tgtgtactca tcacccggat
     2341 tctgtactga gaaactgtgg ctgtttgagg cccgggatct ttctccctgt gaggagtttg
     2401 cccgtgatga ggatgagatt attgaggtgg tgcgtgtggc aaaaagcgag gcggttgcca
     2461 tggtgatgga cgggcgtatc tgtgacggga agacgattgc gcttttgatg cggtgtctga
     2521 tgtgatgcgg tgaatctttt ttctattctg ctcagagcag atccttaatg cttttttcat
     2581 caccggtgct gtcgagtacc tcctgtgctt ccgggtctgc aatcagcagg aagttgggga
     2641 ctatcgcata gacggggaac cggtgaaaga actcctgtac catcagtttg ttcatccgga
     2701 gaatcagaaa ctcccgctgc agaccgatca gctgttccgg tcctgccgct ttcacccggg
     2761 ggagcagatc atctttgagg gctgcaatgt ggctggcatt gagggtttcc atgagagtcc
     2821 ggctttcaat tc
//
LOCUS       DALUAJ010000125         3324 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_22355,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000125 DALUAJ010000000
VERSION     DALUAJ010000125.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22355"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="O0X37_02710"
     CDS             <1..296
                     /locus_tag="O0X37_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HJK61871.1"
                     /translation="VKAELLPLISLRGIGRIRARRLLDRGITTPEELLAADTRDVVAI
                     LGNVLAKNVLEQAAKKTGKTMEIAELPEEKNETVPATASEEAKPQKTLFDFGE"
     gene            297..806
                     /gene="cgi121"
                     /locus_tag="O0X37_02715"
     CDS             297..806
                     /gene="cgi121"
                     /locus_tag="O0X37_02715"
                     /inference="COORDINATES: protein motif:HMM:NF011465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KEOPS complex subunit Cgi121"
                     /protein_id="HJK61872.1"
                     /translation="MMTEQLLTIHEAKATVENTAAVLAQINKIAEKTGSTIVLFDAEK
                     VAGPDHIRSAVRHAERSFASGKPVARTLAMEILLYASGQRQCSLAPKFGLHTGENLLF
                     TVILGGDAAQAQQLLNGIVSFTMHGMTASRATLMEEFGITEDEIAVVGEDRMNELVIE
                     RVALMDAYK"
     gene            complement(827..1945)
                     /locus_tag="O0X37_02720"
     CDS             complement(827..1945)
                     /locus_tag="O0X37_02720"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJK61873.1"
                     /translation="MSCPLCSSEHILPLYSVMNVPAFQNKVYPTESLAESVPCGNISL
                     SFCENCGFVWNATFNPSLLEYDEHYQNEQGYSPVFQSHLQNVCDILLPHLSSKAHIVE
                     IGCGKATFLRKLGELGYNVHGYDPAYEGTDTRIIKEYYSRETVSQHSFGEADLIILRH
                     VLEHIPDPLQFLQSIAEANNYHGLLYIEVPDFRWIIEKNAFYDVFFEHCNYFSLQALT
                     GLFSKVIHYGTFFGDQYCYVIARLADLRAPSMGSYRLLSPPTLDFERCVSAWKDVVLS
                     GSFVIWGAGAKGATFLREIDPTRQTILAVVDINPKKQGKYMPKTAHPVVTPEDLHDLK
                     ISGLIIMNENYLKEISERVWNICGEVPIYVLGKKPIPL"
     gene            complement(2138..2644)
                     /locus_tag="O0X37_02725"
     CDS             complement(2138..2644)
                     /locus_tag="O0X37_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJK61874.1"
                     /translation="MPDLSFSSVKTDAEIAAVAELAQEIWTEHFPPIIGPAQTAYMIE
                     TFQSAKAIHRQITEDCYQYYLLQLGSDRIGYTAVHPEPKNHAMFLSKIYIRKKFRGSG
                     YSRMTMNFIADLCRDDGLNMIYLTVNKQNLSSIAVYEKLGFVRARELVTEIGNGFVMD
                     DYVMEKYL"
     gene            complement(2647..3135)
                     /locus_tag="O0X37_02730"
     CDS             complement(2647..3135)
                     /locus_tag="O0X37_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HJK61875.1"
                     /translation="MPAEHKKDISIQTVVITVSTTRTEKTDLSGKIIRDAFTAADIPV
                     VSVVIVKDDISEIRTAVLEALHVANCIVVNGGTGLTHDDCTIEAVAPLFQKTMDGFGE
                     LFRMKSYEQVGNSVILSRASAGITNGKAIFCIPGSPKAVKLATEEIIIPEIRHILTHA
                     GQ"
BASE COUNT         1013 a          698 c          741 g          872 t
ORIGIN      
        1 gtgtgaaagc agaactcctg ccgctgatct ctctcagggg aatcggccgc atccgtgcac
       61 ggaggttgct cgaccgcgga atcaccacac ccgaagaact gctcgcagct gatacccgtg
      121 atgtggtggc aattcttgga aacgttctcg caaaaaatgt cctggaacag gcggcgaaaa
      181 agacaggaaa aaccatggaa attgccgagc tcccggaaga gaagaatgaa acagttcctg
      241 ctacagcttc agaagaggca aagccgcaaa aaaccctatt tgattttgga gagtaaatga
      301 tgacggagca gttgttaaca atacatgagg caaaagccac ggtggaaaat accgcggcgg
      361 tgcttgcaca gatcaataag attgcagaaa aaaccggatc aacgattgta ctgtttgatg
      421 cggagaaggt ggccggacct gatcatatcc gctctgcggt ccggcatgca gaacgatcgt
      481 ttgcgtccgg aaaaccggtt gcccggacac ttgcaatgga aattcttctg tatgcatccg
      541 gacagcggca gtgttctctt gcaccaaaat tcggtctgca tacaggagag aatctgctgt
      601 ttacggtaat tcttggcggt gatgcagcgc aggcacaaca gctgctgaac gggattgttt
      661 cgttcacaat gcacggcatg actgcatcac gggcaactct gatggaggag tttgggatca
      721 ccgaggatga gattgcggtc gtcggagagg atcgcatgaa tgagctggta attgaacggg
      781 ttgcactgat ggatgcatac aaataaacca ataagaaaaa tgtaaattag agtggtatcg
      841 gttttttgcc gagtacgtat attggaacct caccacaaat gttccaaact ctctccgaaa
      901 tttctttcaa ataattctca ttcatgataa taaggccgga tattttcaga tcgtgcaaat
      961 cctcaggagt aacaacagga tgcgcagttt ttggcatata cttcccctgt ttttttggat
     1021 taatatcaac aacagctaag atagtctgtc gtgttggatc tatttcacgc aaaaatgttg
     1081 ctccctttgc ccctgcaccc caaatcacaa aagaaccaga aaggacaaca tctttccatg
     1141 cagaaacaca tctttcaaaa tccagagtcg gcggggacaa taagcggtat gaccccatag
     1201 atggagcacg tagatctgca agcctggcaa taacataaca gtactgatca ccaaaaaacg
     1261 ttccataatg aattacttta gagaataagc ctgttaaagc ctgtagagag aaataattgc
     1321 aatgctcaaa gaaaacatca taaaatgcat ttttttcaat gatccagcga aaatcaggga
     1381 cttcaatgta caataaccca tgatagttat ttgcttcagc aatcgactga agaaattgaa
     1441 gaggatctgg aatatgttca agaacatggc gtaaaataat tagatcagcc tcaccaaaag
     1501 agtgttgtga aacagtctca cgagaataat attcttttat aattcttgta tctgtccctt
     1561 catatgccgg atcatagcca tgaacattat atccaagttc ccctaatttc ctgagaaagg
     1621 tcgcttttcc acaccctatc tccacaatat gagccttaga agagaggtga ggaagtaaaa
     1681 tgtcacaaac gttttgaagg tgagattgaa acacaggaga atagccttgc tcattctgat
     1741 aatgttcgtc gtattccaaa agcgatggat tgaacgttgc attccataca aatccgcaat
     1801 tttcacagaa agataaagaa atgtttccac aaggaacaga ttcagcaaga ctctctgtgg
     1861 ggtacacttt gttctggaaa gcagggacat tcattacaga gtacaaaggc aaaatatgtt
     1921 cggaagaaca gagggggcag gacattatat aacctctgta tttggaagac tgcaatcaaa
     1981 acattcagag ggagagagga ttaacaaaca gttatgaaat gcacaactaa cataacatac
     2041 attaaacatt ttttaatata tatttgggtc tatatattaa tctattgact gagaagacga
     2101 attccagttc tctttggaag agtaataaag taaaaaatca gaggtacttt tccattacat
     2161 aatcgtccat tacaaatccg tttccaatct cggtcaccag ctcccgggca cgaacaaaac
     2221 cgagtttttc gtacaccgca atggaggaaa ggttctgctt gttcactgtg agatagatca
     2281 tgttcaatcc gtcgtcccga cagagatccg caataaagtt catcgtcatg cgggagtaac
     2341 cgctgccgcg gaactttttt cggatgtaaa tttttgaaag aaacattgca tgattctttg
     2401 gttcagggtg aacagccgtg tagccaatcc gatcagatcc taactgcagg agatagtact
     2461 gatagcagtc ttcggtgatc tggcgatgta ttgctttggc agactgaaac gtttcaatca
     2521 tgtacgcagt ctgtgctgga ccgatgattg gagggaagtg ttccgtccag atctcctgtg
     2581 caagttctgc aacagctgca atttctgcat cagtttttac cgaggaaaag gagaggtccg
     2641 gcataatcac tgacctgcgt gtgtaaggat gtgacgaatt tcagggataa tgatctcttc
     2701 tgttgcaagt tttaccgctt ttggcgatcc cgggatgcag aaaattgcct ttccatttgt
     2761 aattcctgcc gatgcacggg aaaggataac cgagtttcca acctgttcat aactcttcat
     2821 ccggaacagt tcaccaaatc catccattgt cttttgaaac agcggagcaa ctgcttcgat
     2881 agtgcagtca tcatgcgtaa gtccggttcc gccattgact acaatacagt ttgcaacatg
     2941 caatgcctca aggactgccg ttctaatctc agagatatca tccttaacaa tgacaaccga
     3001 aacaaccgga atgtctgctg cagtaaatgc atcgcggatg atcttcccgc tcagatccgt
     3061 tttttctgta cgtgtagtgg agacggtaat cacaacagtc tggatggaaa tgtccttttt
     3121 gtgctcggct ggcatagcaa gtatgtagga actcttcagt attagcaatt cgtattcgaa
     3181 taagttcaat aaaaaacctt gaccatttac aaaaaatgga aaacagatgt ggtgtgataa
     3241 ttttttgatg aggatttttg attctttgcg ggaaactccc ccaccccttt atttcgagtg
     3301 gaacatatgg aagatatctt gcca
//
LOCUS       DALUAJ010000126         1727 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_22611,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000126 DALUAJ010000000
VERSION     DALUAJ010000126.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22611"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="O0X37_02735"
     CDS             <1..381
                     /locus_tag="O0X37_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61876.1"
                     /translation="TRRVSVFSNVYRLPDGRIMYIWYNKNPITGTARPYIAADDGTTH
                     QAYIPPLPDQIRQKYPALNPSDTRMAAAMQIKLAKLLRLPKTDPEPTAEERLSQLADW
                     LAVMYRLTDTVPTAAEQQPVSYPN"
     gene            544..813
                     /gene="gatC"
                     /locus_tag="O0X37_02740"
     CDS             544..813
                     /gene="gatC"
                     /locus_tag="O0X37_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HJK61877.1"
                     /translation="MVSENDVLHIAELADIGIATSELGKFTEQFNAILEYFDILDTLA
                     AEDELDRPLVNVFREDEVRPSLTQEEALANSHNPEDGYIRAPKVI"
     gene            814..>1727
                     /locus_tag="O0X37_02745"
     CDS             814..>1727
                     /locus_tag="O0X37_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HJK61878.1"
                     /translation="MTDPYNAFLSDIEIPSSAAGPLAGIRVAVKDNISTKDIPTTCAS
                     KILLGYIPPYDAHAVELLKAAGATIAGKTNMDEFGMGTTTENSAFGPALNPRDTTRVT
                     GGSSGGSAAAVAAGLVPMALGSDTGGSIRCPASYCGIVGLKPSYGRVSRYGLIAYANS
                     FEQIGPMAANVTDTAKLFSVIAGGDRRDSTSVHKPYNFADLHADIKGKIIGVPKEYFG
                     EGVDADVAKTVETSIGKLEELGAVIHEVSLPSMQYALAAYYVTCTCEASSNLDRFDGV
                     RYGPEPEMNLPWHDAYTKVREAGFGPEGR"
BASE COUNT          459 a          535 c          389 g          344 t
ORIGIN      
        1 accagaagag tgtccgtctt cagcaatgtt taccgtctcc ccgacggcag aatcatgtat
       61 atctggtaca acaaaaaccc gatcacagga acggcacgtc cctacattgc agcggatgac
      121 gggacaacac accaggcata catacctccg ctgccggatc agattcggca gaaatatcct
      181 gctctgaacc cttcagacac ccgcatggcc gcagcaatgc agatcaagct tgccaaactt
      241 ctcagactgc caaagacgga tccagaacca accgccgagg aacgactatc ccagcttgcc
      301 gactggttag ctgttatgta ccgcctcacg gatacagtac ccacagcagc cgaacagcag
      361 ccagtcagct atccgaacta aagaccacat ccgcactata ataatacacc ccaactgctc
      421 tgcgccggac caaggatcac cggaactttt tccaaaaaat ggatcgatcc tgccggaaga
      481 gtagtaactc tttttaccca aaacaacatc atcactaaac tactattcac aggtcgcaga
      541 aatatggtct ctgaaaacga tgttctccat atcgcagaac ttgccgatat cggtattgca
      601 acgtccgaac tcggcaaatt caccgaacag ttcaatgcaa ttcttgaata cttcgatatc
      661 ctcgacaccc ttgccgcaga agatgaactt gaccgtccgc tggtcaatgt attccgcgaa
      721 gacgaggtca gaccctctct tacacaggag gaggcccttg caaactcgca caatcccgag
      781 gacggataca tccgcgctcc caaggtgatc taaatgaccg acccgtacaa tgcattcctc
      841 tcagacatcg agataccctc ctcagccgcg ggaccacttg ccggtatccg cgtcgctgtc
      901 aaagataaca tctccaccaa agatatcccc actacctgcg cctccaaaat ccttctcgga
      961 tacatcccgc catatgatgc ccatgcagtt gaactgctca aggcggcagg ggcgacaatc
     1021 gcaggaaaaa ccaatatgga cgagttcggt atgggaacca ccacggagaa cagtgctttc
     1081 ggtccagcat taaaccctcg cgacacaaca agagtcaccg gaggatcctc cggaggttcc
     1141 gcagcagcag tggccgcagg tcttgttccg atggcactcg gatcagatac cggcggatca
     1201 atccgctgcc ctgcatccta ctgcggtatt gtcggcctca aaccctctta cggccgtgtc
     1261 agccgctacg gtttgattgc ctatgccaac tcctttgaac agatcggccc gatggcagca
     1321 aacgtcaccg acaccgcaaa actgttcagt gtcattgccg gcggtgaccg ccgtgactca
     1381 acatcggttc acaaacccta caactttgcc gacctccacg cagatatcaa aggaaagatc
     1441 attggtgttc ccaaggagta cttcggtgaa ggtgtggatg cagacgttgc aaagaccgtg
     1501 gaaacatcca tcggtaaact cgaagagctg ggcgcagtca tccacgaggt ctcacttcct
     1561 tccatgcagt atgctctcgc cgcttactac gttacctgca cctgtgaggc aagctccaac
     1621 ctggaccggt tcgacggtgt cagatacggt cccgaacctg agatgaacct tccctggcac
     1681 gacgcctaca ccaaggtccg cgaagcaggc ttcggcccgg aaggccg
//
LOCUS       DALUAJ010000127         1611 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_23036,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000127 DALUAJ010000000
VERSION     DALUAJ010000127.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23036"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /locus_tag="O0X37_02750"
     CDS             complement(<1..1081)
                     /locus_tag="O0X37_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel protein"
                     /protein_id="HJK61879.1"
                     /translation="MMERDNQPINLKDVLIEMKDISELMVDLAYSAVLFESNAIADEV
                     IELEERMNDLVYQARITSMMSVRRLEDTEPMSGLLQLAEASERISNQAADIAKNIMRH
                     VSFPAKLRKALPEAEEATHRTVVAAGSTLDCARLGDIKLQSATGIRIIMIRRMRQRIY
                     DPDKHTVLRAGDVLVGRGPEYGFPALCELAGQPAPNDDDDVATAISDLDRAAALMIEM
                     KNISELSVGLAYTALLHENMDLANEVVALEEELDEMRLKLDLWTLEAAKRTEDVASLR
                     GMLYMSSFAESISDAASSIADVVLREIDVPPIIKRIVRESDEIISWVTVHPGSPLDKK
                     SLADSHVGTVTGMVIFALKHENRWTY"
BASE COUNT          384 a          411 c          403 g          413 t
ORIGIN      
        1 ggtaggtcca gcggttttca tgtttgaggg caaagatgac cataccagtg accgttccca
       61 catgcgagtc ggcaagggat tttttatcca gcggggaacc gggatggacc gtgacccaag
      121 agatgatctc atccgattca cggacaatcc ttttgataat cggcggaacg tcgatctccc
      181 ggagaactac atctgcaatc gaacttgccg catcggagat ggattcggca aacgaagaca
      241 tgtagagcat tccgcgaagg cttgcaacat cctccgtacg ctttgcagct tcaagtgtcc
      301 agagatcaag tttgaggcgc atctcatcaa gttcctcttc cagagcaaca acctcgttgg
      361 cgagatccat gttttcatga aggagtgccg tgtatgcaag gccgacggag agttcactga
      421 tgtttttcat ctcgatcata agggctgccg cccgatcgag gtcgctgatg gcagtcgcga
      481 catcgtcatc atcatttggc gcaggctgac cggcaagttc gcagagtgca gggaaaccat
      541 actcaggccc cctcccaacc agcacatccc ctgcgcgaag cacggtgtgt ttgtcaggat
      601 cgtagattct ctgccgcatc cggcgtatca tgataatacg aataccagtt gcggactgga
      661 gtttaatgtc gccaagacgc gcacagtcaa gggtactgcc cgccgcaaca acggtacggt
      721 gggttgcctc ttctgcctcc ggcagtgctt ttctgagctt tgccgggaag gaaacgtgcc
      781 gcatgatatt ttttgcgata tccgcagcct ggttggagat acgctccgag gcttctgcaa
      841 gctgcaggag accgctcatc ggttcagtgt cctccagacg gcgtacgctc atcatactgg
      901 tgatacgtgc ctgatatacg aggtcgttca tccgctcttc aagttcgatg acttcgtctg
      961 caatggcgtt gctctcaaag agtaccgcag agtaagccag atcaaccatc agttccgaga
     1021 tgtccttcat ttcaatgagg acatccttga ggttgatggg ttgattatcg cgttccatca
     1081 tgccttacta ttataatgga gacgtctgac tacttaagtc aaatcctcct agtcagtttg
     1141 tcaatattat tggaaaaatt gtctctgagt ttgttttaag gcactttcat tctgcacggt
     1201 tgagggttta taatcctatc tgccttactg tcaccatgag gtgtaagcat tcatgatccc
     1261 tgcaatgtta ccatcagagt acctgggtta ctatgccagg gaaagtacac ggatgtcact
     1321 tcagaactcc ttgagacgtt atattaccta tgtcacagga gaggaatgta cagacgttga
     1381 tgccagctgg tccaattatc tcaatgaaag agatgcagct gcaattgcct ctgatctaat
     1441 gaatttttac agatttcccg gggtatcgga tcttgcgccc ctgaccgtga taacatattt
     1501 gtcgggcgct gagggatatc tccgtgacac ctgtgaaatt tccctaaccc ctctgcaaaa
     1561 acggctgcga cgcaagcgga cccctgagag accgcgccca ctaacacagg a
//
LOCUS       DALUAJ010000128         2006 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_23127,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000128 DALUAJ010000000
VERSION     DALUAJ010000128.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23127"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="O0X37_02755"
     CDS             complement(<1..386)
                     /locus_tag="O0X37_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HJK61880.1"
                     /translation="MAFGAIILFCAVVLDRLIGDPRSRLHPVALLGTVIGWWGKTNTY
                     PKRLERITGIVGWLITVLIFILPFLVICLIPETYWYIQLPLAAVLLSFCFASRSLEEH
                     VAAVETALGKGDIEGRAAVQMLVSRDV"
     gene            complement(368..913)
                     /locus_tag="O0X37_02760"
     CDS             complement(368..913)
                     /locus_tag="O0X37_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61881.1"
                     /translation="MAIVEIQLNRLGINSLELSTDAVDVSAGTALHVRFVNHGSPTHA
                     TLRCEASAYTDFTYENIYVEGESELEIKMKEEAGTGSFDMQVITGYGMRREAFTIHVL
                     KSCPAPVPEPVIFPEEPTEDPKKGSFGQKAAMILIMPIIAAIIIALWQFGLLNIDGHA
                     MTIIVYLVMLAGVIVAWRSAQ"
     gene            complement(925..1104)
                     /locus_tag="O0X37_02765"
     CDS             complement(925..1104)
                     /locus_tag="O0X37_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methytransferase partner Trm112"
                     /protein_id="HJK61882.1"
                     /translation="MKRWVLDIICCPVCKGNFMLTEMEGNDTDIVEGLLTCTSCKRVY
                     PISSGIANLLPNQED"
     gene            complement(1108..>2006)
                     /locus_tag="O0X37_02770"
     CDS             complement(1108..>2006)
                     /locus_tag="O0X37_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011842861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HJK61883.1"
                     /translation="PNAGHTVEVGDKKYGVRMVPSGFVYPHARLMIGSGVLVDPRVFA
                     HELEVLGCRDRTFIDGRCGIIEEDHIYKDKHDDHLSKTVGSTGSGCGPANSDRVMRTA
                     RLAKDVPELAPYIIDVAQNVNDAIDAGDSVIIEGTQGFGISLYYGNYPFVTSKDTSAS
                     QMAADVGVGPTKIDDVVVVFKAFPTRVGEGPFPTELTHEQSLAMGIQEFGTVTHRERR
                     IGTWDGKMARYSAMINGCTAIAITGVDHIDKDVFGVKEYSKLTPKVREFLEQIESDTG
                     CPVGIISTGPEQSQIIDIRSEL"
BASE COUNT          471 a          553 c          503 g          479 t
ORIGIN      
        1 acatcgcggc tcacaagcat ctgtaccgct gctcttccct cgatatctcc ctttccgagt
       61 gccgtctcaa cggcagctac gtgctcctcg agtgatcgtg atgcaaaaca gaatgacagc
      121 agtactgctg caaggggcag ctgaatatac cagtatgtct ctggaatcag acagatcaca
      181 aggaacggca ggataaagat gagaacggtt atcagccacc ctacaatacc ggtgatccgt
      241 tccagccgtt ttggataggt atttgttttt ccccaccagc cgatcacagt tcccaggaga
      301 gctaccgggt gcaggcgcga tcgcggatca ccgatcagcc gatccagaac tactgcacaa
      361 aaaaggatta ttgcgccgaa cgccatgcaa cgatgactcc ggcgagcatt acaaggtaca
      421 caataattgt catggcgtgt ccgtcaatat tcaggagacc gaactgccac aatgcaataa
      481 tgattgcagc gatgattggc ataatcagta tcatcgctgc tttttgacca aaggatcctt
      541 ttttcggatc ctctgtcggt tcttccggga agataaccgg ttccggtacc ggggcaggac
      601 aggacttcag cacatggatc gtgaacgcct cgcggcgcat accatagccg gtaatgacct
      661 gcatgtcgaa acttccggtt cccgcctcct ccttcatctt gatctcaagc tccgactcgc
      721 cctcaacgta gatgttttcg tatgtaaagt ccgtgtaagc cgatgcctcg caccgcagtg
      781 ttgcgtgtgt cggagatccg tgattcacaa accggacgtg cagtgcggtt cctgcgctta
      841 catccacggc atcggtggat agttcaaggg aatttatccc cagacggttc agctgaattt
      901 caacaattgc catagtagag cacctcagtc ctcctgattt gggagaaggt ttgcaatgcc
      961 ggaggagatc ggatacaccc gcttgcagga tgtgcaggtc agcagtcctt cgacaatatc
     1021 tgtatcatta ccctccatct ctgtaagcat aaagtttccc ttgcagacag gacagcagat
     1081 aatatcgagg acccagcgtt tcatggttta cagctcggat ctgatatcaa tgatctggga
     1141 ctgttcagga ccagttgaga taattccgac cgggcaacct gtgtcagact cgatctgttc
     1201 aaggaactca cggaccttcg gtgtgagttt gctgtactct ttgacaccaa acacatcctt
     1261 gtcgatgtgg tcaacaccgg tgatggcgat ggccgtacat ccgttgatca ttgcggagta
     1321 acgtgccatc tttccgtccc atgtaccgat tctccgctcg cggtgcgtaa cggtaccaaa
     1381 ctcctgaata cccattgcaa gggactgttc atgggtgagt tcggtgggga acggtccttc
     1441 accgactctg gtcgggaatg ccttgaagac cacaaccaca tcatcgattt tggtcggccc
     1501 gacaccgaca tcggctgcca tctgcgatgc ggaggtgtct ttacttgtca cgaacggata
     1561 gtttccatag tagagggaaa taccgaaccc ttgcgtaccc tcgatgataa ccgagtcacc
     1621 cgcgtcgatt gcatcgttca cgttctgggc aacatcaatg atgtacgggg caagttcggg
     1681 aacatccttt gcaagacgtg ccgtgcgcat gacacgatcg gagtttgccg ggccacagcc
     1741 ggagccggtt gatccaaccg tcttagagag gtggtcatcg tgtttgtcct tgtaaatatg
     1801 atcctcttcg atgattccac aacggccgtc aatgaaggtc cggtctctgc atccaagcac
     1861 ctccagctcg tgggcaaaga cccgcgggtc aacaagaact ccgctgccga tcataagtct
     1921 tgcgtgggga tacacgaaac ccgacggcac catccgcaca ccgtatttct tatctcccac
     1981 ctcgacggtg tgaccggcat tcggcc
//
LOCUS       DALUAJ010000129         1714 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_23437,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000129 DALUAJ010000000
VERSION     DALUAJ010000129.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23437"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="O0X37_02775"
     CDS             <1..909
                     /locus_tag="O0X37_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide amidohydrolase"
                     /protein_id="HJK61884.1"
                     /translation="DVLIVRGNFRAASSGIDGGIADVKTILNITVPRNFSGDAALEIN
                     RVATEQGFLQPQFGLLTAVPITNLCIARYDYITVFVTAGVSENNRTINIIVTSDRPLS
                     DTALLGAMITVTETKMQVLADRKLPAGASPTDAVVIAAEKSRSVPEMFAGVLTDTGER
                     IAKAVRQALTEALIRFDNYLLSTWGISRGWSRGSSSIVRRTRPSFFIYSRYGGDHWTE
                     WVPEGCPYYPCHNYPRQQCSFCYCPLYPCMDETLGTMIETPHGEIWSCMDCCLVHIPE
                     VADHLLKNPEAGVLELKALAKKLNEK"
     gene            complement(917..1102)
                     /locus_tag="O0X37_02780"
     CDS             complement(917..1102)
                     /locus_tag="O0X37_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HJK61885.1"
                     /translation="MAITIDSTIADLLREKPESAAILQSFGMGCLGCAIANNETIREA
                     AMVHGIPLEELAKKLGI"
     gene            1189..>1714
                     /locus_tag="O0X37_02785"
     CDS             1189..>1714
                     /locus_tag="O0X37_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61886.1"
                     /translation="MKPMNLLEINMDQNQIQQITDRISHKLESKGVSPDRQSITDKLS
                     SYINEFGVVPYEAERKVLSDQMRLFDIPEDDVSSAPSSVPEGGVYSLSDIQPGEWVTV
                     EVKVVSLQTPSSPSISQTGVLADSSGAVRFVVFSKASEIPPLELEQWYRIESAVVDSF
                     REVPSLKLHSGSRVT"
BASE COUNT          391 a          416 c          460 g          447 t
ORIGIN      
        1 gatgtactga tcgtccgcgg gaatttccgt gcggcaagca gcggtattga cggcggcatt
       61 gccgatgtca aaacaatcct gaacatcaca gttcccagga atttttccgg tgatgctgcg
      121 cttgaaatca atcgggttgc aacggaacag ggatttttac agccgcagtt cgggctttta
      181 acggctgtcc ccataaccaa tctctgtatt gcccgctatg actacatcac tgtctttgtc
      241 accgcaggtg tttcggagaa caaccgcacg atcaacatta ttgtcacctc cgatcgcccg
      301 ctttcggata cggcactgct tggcgcaatg atcactgtga cagaaacaaa gatgcaggta
      361 ttggcggatc gaaagcttcc tgccggtgca tcacccacgg acgccgtggt gattgctgcg
      421 gagaaatccc gttcggtgcc tgagatgttt gccggggttc ttaccgatac cggtgaacgc
      481 attgcaaaag ccgttcgcca ggcattgacc gaggcactga tacgttttga caactatctc
      541 ctttccacat ggggtatctc ccgcggatgg tcacgtggtt catcctctat tgtccggaga
      601 acgcgtccct cgtttttcat ttacagcagg tatggcgggg atcactggac ggagtgggtc
      661 ccggaaggct gcccgtatta tccgtgtcat aactatccgc gtcagcagtg cagtttctgt
      721 tactgtcccc tttacccctg catggatgag acgcttggga caatgatcga aacaccacac
      781 ggagagatct ggagctgtat ggattgttgt ctagttcata taccggaagt tgcggatcat
      841 ctgctgaaaa atccggaagc cggtgtattg gaactgaaag ctcttgcaaa aaagctgaat
      901 gaaaaataaa atatttttag atgccgagct ttttggcaag ctcttctaag gggattccgt
      961 gaaccattgc ggcctcgcgg atggtctcgt tgtttgcgat tgcacagcca aggcatccca
     1021 tgccgaagct ctggagaatt gcggcacttt ccggcttttc gcggagcaga tcggcgatag
     1081 ttgaatcgat ggtaattgcc atgtatacta cattggcaat tcgactactt gatgtttccg
     1141 aacagggcac cggcactttc agtacgcatg gtcgggggtt aatatagtgt gaagccaatg
     1201 aatctgttgg agatcaatat ggaccaaaat cagatccaac agattaccga cagaatctcc
     1261 cataaattag aatccaaagg agtttcgccg gacagacaga gcattacgga taaactctca
     1321 agctacatca atgagttcgg tgtggtgccg tatgaggctg aacgaaaggt tttgtccgat
     1381 cagatgcggc tgtttgatat tcctgaggat gatgtatcct ccgcgccgtc ctcggtgccg
     1441 gaaggcggtg tctattctct ttccgatatt cagccgggtg agtgggtcac cgtcgaggtg
     1501 aaggtggtgt ctttgcagac accgagttcc ccgtcaattt cacagaccgg tgttcttgct
     1561 gacagctccg gtgcggtacg gttcgtggtg ttttcaaaag cgagtgagat tcctccgctg
     1621 gaacttgaac agtggtaccg gattgagtcg gcggttgttg attcgttccg cgaggttcca
     1681 agtctgaaac ttcactcggg ctcccgcgtt acac
//
LOCUS       DALUAJ010000130         2105 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_23471,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000130 DALUAJ010000000
VERSION     DALUAJ010000130.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23471"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="O0X37_02790"
     CDS             complement(<1..911)
                     /locus_tag="O0X37_02790"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HJK61887.1"
                     /translation="MGEPYYIGGQPTYSPNTVTVIFPYTGEAFTEVCIAGMEDQARAA
                     NCARQGFAETKTLPAHRRAEILQRVADKIEEERETFVDILIHESGKTVRQAHFEVTRA
                     VSVMRTSAEESVRLTGEVIPLDKTEAGEGYTGYYYKVPSGPVLCITPFNYPLNLACHK
                     IGPAIAAGCSFVLKPSTKTPRSALLLGKIILEAGYPAKAVNVVPCTGSSAETLVRDPR
                     FSVLSFTGSPAIGWRLKELFPGRHIGLELGGNAAAVVHKDADLTYAARRIAEGAVSNA
                     GQSCISVQRVYLHADIYDTCLAMILNN"
     gene            1049..1157
                     /locus_tag="O0X37_02795"
     tRNA            join(1049..1085,1119..1157)
                     /locus_tag="O0X37_02795"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1082..1084,aa:Tyr,seq:gta)
     gene            1188..1260
                     /locus_tag="O0X37_02800"
     tRNA            1188..1260
                     /locus_tag="O0X37_02800"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1308..1380
                     /locus_tag="O0X37_02805"
     tRNA            1308..1380
                     /locus_tag="O0X37_02805"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1342..1344,aa:Asp,seq:gtc)
     gene            complement(1505..1882)
                     /locus_tag="O0X37_02810"
     CDS             complement(1505..1882)
                     /locus_tag="O0X37_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit beta"
                     /protein_id="HJK61888.1"
                     /translation="MANPQAGIPPQIQQQLAMFQQIQQQLQQVSSQKMQYEMTLRETK
                     RALEELNAAADDVVVFSSAGSILIQKKKETVSTELQEKIDSLELRIGSLDKQEKTMAT
                     KASQLQKQIQDAVAGAGSIPTAE"
     gene            complement(1885..>2105)
                     /locus_tag="O0X37_02815"
     CDS             complement(1885..>2105)
                     /locus_tag="O0X37_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="KEOPS complex subunit Pcc1"
                     /protein_id="HJK61889.1"
                     /translation="AEFRFVCPYAEKLYAVLAPEAGSDPGEKSRVRISRDGDAVTLFV
                     EAEDASALRASLNMWLRLVNVCKEVLEL"
BASE COUNT          467 a          556 c          493 g          589 t
ORIGIN      
        1 atattattca gaatcatggc aagacaggtg tcgtaaatat cggcgtggag ataaacccgc
       61 tgtacactaa tacagctctg gcccgcattg gatactgctc cttcggcaat tctccgtgcg
      121 gcatatgtga ggtctgcgtc tttatggaca accgccgctg catttcctcc cagctcaaga
      181 ccgatatgcc ttcccgggaa caactctttt agtctccagc caattgcagg gcttccggta
      241 aaggacagta ctgaaaaacg tggatcgcgt accagcgttt cggcactgga tcccgtacac
      301 gggaccacat tgaccgcctt tgcgggatat cctgcttcga gaataatctt gccgagcagg
      361 agggctgacc gtggtgtttt tgtggaaggt ttgagaacaa aagagcaccc tgcggctatt
      421 gcgggtccaa ttttgtggca ggcaagattc agaggataat tgaacggggt gatgcacaat
      481 accggtcctg aaggtacttt gtagtagtaa ccggtatacc cttcgccagc ctctgtttta
      541 tccaacggga tcacctctcc agtaagccgg acactctctt ccgcggatgt ccgcatcact
      601 gatactgcac gtgttacctc aaaatgtgcc tgacgaaccg tttttcccga ctcgtgaatc
      661 agaatatcca caaacgtctc gcgttcctct tctattttgt ctgcaactct ttggaggatc
      721 tctgcgcggc ggtgagccgg cagagttttt gtttcggcaa atccttgtct tgcacagttt
      781 gctgcgcggg cctgatcctc cataccggcg atacatactt cggtgaatgc ttctccagta
      841 tagggaaata ttacggtaac tgtgtttgga ctatatgttg gctgaccccc aatgtagtat
      901 ggttctccca ttatctcaac tcccctgggt ttcttttgtt ctatctcttc tgctctatct
      961 attaaagagt taggaatctg ccaatgaatc tgctctacaa aatcgtcata tttatatttt
     1021 caaacagcat tcttattaaa gcagtcatcc cgacttaact cagatggtag agtgcgcggc
     1081 tgtagtaaga ttcacgcctg cgggcgtact gcgcggctac cgcgatgtcc ccggttcgaa
     1141 tccgggagtc gggaccattt tcatgaccgc agtcaaaaac ctcctgtgct caagtagtgt
     1201 agtggcctat catgatgccc tgtcacggca tcgactcgga ttcgaattcc gacttgagcg
     1261 tttatgtttt tgattgctgt cgagggatga cctgccaaca tatctgtgct caagtagtgt
     1321 agtggcctat catgatgccc tgtcacggca tcgactcgga ttcgaattcc gacttgagcg
     1381 ttttcttttc ccaatatttc agattgtttt gtctgatctt tcgtacctgt ttcctacggt
     1441 ctctatccgg aaacgaccgc cggatcgcca gagtatcttg tgaaaaccaa gtaaaaaaag
     1501 aaaattattc tgcggttggg atgctgcctg caccggcaac agcatcctga atctgttttt
     1561 gcagctggct tgcctttgtt gccatcgtct tctcctgctt gtcaagagag ccgatacgca
     1621 gctcaaggga atcaatcttc tcctgaagtt cagtcgaaac cgtctctttc ttcttctgga
     1681 ttagaattga tcctgccgag gagaatacaa caacgtcatc tgctgctgcg ttgagttcct
     1741 ccagcgcacg tttggtctcg cgaagcgtca tctcatactg catcttctgg gaagatacct
     1801 gctgcagctg ctgctgaatc tgctgaaaca ttgccagctg ctgctgaatc tgcgggggaa
     1861 tgcccgcctg tgggtttgcc atttttacaa ctccaatact tctttacaca cgttcactaa
     1921 ccgaagccac atatttagtg atgcacggag agccgatgcg tcctccgcct ccacaaagag
     1981 tgtcacggca tctccatctc gggaaatccg tacacgggat ttctctcccg gatccgatcc
     2041 tgcctccggc gcaagaaccg catacagttt ctccgcgtaa ggacacacga accggaactc
     2101 tgcgg
//
LOCUS       DALUAJ010000131         1737 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_24192,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000131 DALUAJ010000000
VERSION     DALUAJ010000131.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24192"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="O0X37_02820"
     CDS             complement(<1..590)
                     /locus_tag="O0X37_02820"
                     /inference="COORDINATES: protein motif:HMM:NF025825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HJK61890.1"
                     /translation="MGEDVAGSSMPLDILNTFYSGENPLCVMQNLSEDQNRRKIIIVS
                     NQRLMYFEENATGMYDETLYAFARIDSVIFHEGKKSSELKIVDTDGSILKIGWLLNEE
                     AQRVLLTLQSAMNDLGTAMVSLDRRKPMFSGEEWILKKPLDYLTKTVKSDAPPMTESY
                     IAPILPKDEAEDCDGSQELFEGEDDVIPASVVQPKDG"
     gene            812..>1737
                     /locus_tag="O0X37_02825"
     CDS             812..>1737
                     /locus_tag="O0X37_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HJK61891.1"
                     /translation="MYPPELVAILILLLVPLFAAVLLAVLNRDSIRNGIVGVVSAVLI
                     AASLYLAYLAWSSPAPITIALESAKVISVGLFIVELVVALAIIALSLKYRRILPLALA
                     LIQLALLLVLELVYAESVIISTAFTVNNLSAIMVLIIGIIGTLICVYALGYMKDYHHH
                     HTEVADRKPYFFAMLFTFLTAMFGIVLSDSLMWILCFWEVTTLCSFLLIGYSKTPEAV
                     TNSFRAVWMNLIGGIAFTLAIISLLETDPFGATLLSITDIISYHDMAALTIPAALIAI
                     AGLTKAAQMPFSTWLTGAMVAPTPVSALLHSST"
BASE COUNT          421 a          473 c          372 g          471 t
ORIGIN      
        1 ccatcctttg gctggaccac ggatgcaggg attacatcat cctccccctc aaacagttcc
       61 tgcgaaccgt cacaatcctc tgcctcatct ttgggaagga taggggcgat gtaactctcg
      121 gtcatgggag gagcatctga ttttaccgtc ttggtcaggt aatcaagagg ttttttcagg
      181 atccactcct cgccggaaaa catcggcttt ctgcgatcaa gggataccat agcagtccca
      241 agatcattca ttgccgattg cagcgtcaga agaacccgtt gcgcttcctc attcaagagc
      301 cagccaatct tcagtatcga accgtcagta tcgacgattt tcagctcaga ggattttttg
      361 ccttcatgaa aaataacaga atcaatacgg gcaaacgcgt acaacgtctc atcatacatg
      421 ccggtcgcgt tctcctcaaa gtacatcaaa cgctgattag aaacgatgat aatttttctg
      481 cggttttgat cctcggaaag gttttgcatg acgcacagag gattttcacc ggagtaaaag
      541 gtattcagaa tatccagggg catcgaactg cctgccacat cctcacccat aacggttgat
      601 tgtttgttgt tgcaggtctt atgccttctt atagagcaat accgcggcac aatatatcga
      661 aaggttcagc agatggagtt tacaaaaagg catgtgaatc accaaaagga cagggtttcg
      721 aagagaaata caaaacggcc ccatatcctc gcctggaaga tattagaatg attaataatg
      781 atcaataccc atactttcaa caagtgatat tatgtacccg ccggaactcg tagccatcct
      841 catactgctg ctggttcccc tgtttgccgc agttcttctt gcggtattga acagggactc
      901 cattcgcaac ggtatcgtcg gtgttgtttc ggcagttctt attgcggcat ccctctacct
      961 tgcctatctt gcatggtcaa gtcctgcccc gataacgatt gccctggaat ctgccaaagt
     1021 gataagcgtc ggcctcttca tcgtagagct cgtggttgcc cttgcaatca tcgcactttc
     1081 tctcaaatac cggcggattc ttccgcttgc tctcgcccta atccagcttg ccctgctgct
     1141 tgttctggag ttggtatatg ccgagtccgt catcatctca acagcattca ccgtgaacaa
     1201 tttgtcagcc atcatggtgc tgatcatcgg catcattgga acactcattt gtgtgtatgc
     1261 cctcggctac atgaaagact atcaccacca tcatacagaa gttgcagacc gcaagccgta
     1321 cttctttgca atgctcttta ccttcctgac agcaatgttt ggaatcgttc tctcagatag
     1381 tctcatgtgg atcctctgct tctgggaagt gacaacactc tgttcattcc tcctcattgg
     1441 atactcaaag acacccgagg cggtcaccaa ctcattccgt gcggtatgga tgaacctcat
     1501 cggagggatt gcattcacgc ttgcgatcat ctctctcctg gaaactgatc cgtttggggc
     1561 aacactcctc agcatcacag acattattag ctaccatgac atggcagccc tcaccattcc
     1621 tgcagcactt atcgccattg ctggtcttac aaaagcagcg cagatgccgt tctcaacatg
     1681 gctcaccggt gcaatggttg ccccgacgcc ggtctccgct ctgctgcact caagtac
//
LOCUS       DALUAJ010000132         3315 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_24316,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000132 DALUAJ010000000
VERSION     DALUAJ010000132.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24316"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="O0X37_02830"
     CDS             <1..344
                     /locus_tag="O0X37_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HJK61892.1"
                     /translation="DKATDSTKKALRPPLLDVDIAGATAALINVIGGPDMTMFEAEGV
                     VQEVYQRIDPGARIIWGVQVDPEMEGRMRTMLIVTGVQSPQIYGKQEQTYTASQSSQV
                     QKSKFEIDFLR"
     gene            407..592
                     /locus_tag="O0X37_02835"
     CDS             407..592
                     /locus_tag="O0X37_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase SEC61 complex subunit gamma"
                     /protein_id="HJK61893.1"
                     /translation="MPEVSKQSISEFFRKYLRVLKLARRPTKDEFFKISAVAAAGIAV
                     IGVLGFIIYLVFHYLLP"
     gene            597..1073
                     /locus_tag="O0X37_02840"
     CDS             597..1073
                     /locus_tag="O0X37_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor Spt5"
                     /protein_id="HJK61894.1"
                     /translation="MTDSEFGNHYYIIKTTSKHERTVADNIREAIENNMTDVVVTAVV
                     VPEDIKGYVFVESPEEHARIEELVETIAHARVVLKSETSMEEIKHFLVPKPAVSGIEE
                     GTIVELIAGPFKGEKAVVKRVDHTKEEITVELYESIVPIPITVRGDNVRVIDRSHE"
     gene            1357..1830
                     /locus_tag="O0X37_02845"
     CDS             1357..1830
                     /locus_tag="O0X37_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HJK61895.1"
                     /translation="MAETVEVLVPGGRATAGPPLGPALGPLGINVKAVVDDINKKTAE
                     FNGMSVPVTVTVDDKKNVTLTVGIPPTTALVMKEAGIEKGSGTPNTQAVGNLPLEAVI
                     RIAKMKMESMLSYDLKNAVKEVMGTCVSVGVTIDGKTAKAAIAAVNAGEYDAQLA"
     gene            1953..2594
                     /locus_tag="O0X37_02850"
     CDS             1953..2594
                     /locus_tag="O0X37_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HJK61896.1"
                     /translation="MVDKTQIISAVTAAMEQAPKRKFQESIDITINLKHVDMAQPKNR
                     IDETILLPQAIGVKKIAVLGKGDIVSQARNAGVDIIIGPEEIERLGGAPREARKLAGQ
                     YDYFLAETAVMPLVGRWLGQRLGPRGKMPQPIPMGQDITPIVERLRNSVKIRSKDRLN
                     MSVKIGNTAMSAEAVAENVDAVLKRVLGRLENGELNIRSVYVKTTMGPAVKVM"
     gene            2597..>3315
                     /locus_tag="O0X37_02855"
     CDS             2597..>3315
                     /locus_tag="O0X37_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HJK61897.1"
                     /translation="MAIYTHHLPAWKREEVESIVENAGKHELIGLVDVYGIPARQFQQ
                     IRRNLHGSAVVKVARNTLVEHAFNELGGHFLDLNGHVSEHSALIFADGNPFKLFKSLE
                     QTKTKRSAKAGEVAPEDIVVPAGPTSFKPGPIVGELQQAGIPAAIDGGKVKIKETKTV
                     VKAGQAISKKQADVLAKLDIKPMPVGLSLLAVVYEGEIYLPDVLGIDEEAYKAKITLA
                     AQQVFNLAVNAAIPTPCAQIT"
BASE COUNT          929 a          797 c          864 g          725 t
ORIGIN      
        1 gggacaaagc aaccgactct accaagaaag cactccgtcc cccactgctg gatgttgaca
       61 tagccggcgc aaccgccgca ctcatcaatg taatcggcgg tccagacatg acgatgtttg
      121 aggcagaggg tgttgtacag gaagtttacc agcggattga tcccggagca cggattatct
      181 ggggagttca ggttgacccg gagatggaag gccgcatgcg gacaatgctt attgtaacgg
      241 gtgttcagtc cccacagatt tatggtaagc aggagcaaac atatacggca tcacaatcaa
      301 gtcaggttca gaaatccaag tttgagattg acttcctgag gtgaatatat tggcagatga
      361 aaaaaatact gaaaagaagg cagcatcctc tgctgtaaac gtaaagatgc cggaggtctc
      421 caaacagagt atctccgagt tcttccgcaa gtacctgcgt gtattaaaac ttgcacgcag
      481 accgacaaag gatgagttct tcaagatctc cgcagttgcc gcagccggta ttgcggtaat
      541 cggagttctc ggtttcatca tttacctggt cttccactac ctgctcccgt gaaactatga
      601 ctgattcaga gtttggcaac cattactaca taattaaaac aacatccaag cacgagcgga
      661 cggttgcaga taatatccgc gaggcaatcg agaataacat gacggatgtt gtcgtgacag
      721 ctgtagtggt tccggaggat attaagggct atgtctttgt ggaaagccca gaggaacacg
      781 cgcgcattga agagctggta gagactattg ctcatgcacg tgttgttctg aaaagcgaaa
      841 cgtcgatgga agagatcaaa cacttccttg tacccaaacc ggcagtctcc ggtatcgaag
      901 aaggaacaat tgttgaactg atcgcagggc cgttcaaagg tgaaaaggca gtggtcaagc
      961 gtgtagacca caccaaagaa gagatcaccg ttgaactgta tgagtcaatt gttccaattc
     1021 caatcaccgt gcgcggcgat aatgtgcgcg taatcgaccg tagccacgag taaatcccaa
     1081 tccatttttt agatcctgaa ataggtctgc gcgatctgtt cgcagcaata tctctccagt
     1141 gtatgttaaa atatatttca gagatagaaa ttgctttgaa gaaccagata atccgttaac
     1201 aacaccattt gtacaaacgg gaatagacgt tgcagaatac actgcactac ccaagaagaa
     1261 ccctatcacc cactaccttt atgttctcat acgtcgacct tattgaacca atgttaggca
     1321 gacctttgaa ggtctgtact gacatacggt gatacaatgg cagaaactgt cgaggtactg
     1381 gtacctggcg gtagagcaac tgcaggacca ccacttggtc cagcactggg tccccttggg
     1441 atcaacgtga aggcagtcgt tgatgacatc aacaagaaga ctgctgagtt caacggtatg
     1501 tctgttccgg taacggttac agtcgatgac aaaaagaacg tcacgttaac agtcggtatt
     1561 cccccgacaa ccgcacttgt tatgaaggaa gcgggaatcg agaagggatc cggcactccg
     1621 aacactcagg cagttggtaa tctgccgctt gaagctgtca ttcgcattgc aaagatgaag
     1681 atggagagca tgctttccta cgacctgaaa aacgcggtca aggaagtcat gggcacctgc
     1741 gtttccgttg gtgtgaccat cgacggcaag acggcaaaag ccgcaattgc cgcagtcaat
     1801 gcaggcgagt acgacgcaca gcttgcataa agttctgtgt ccggcttcaa acgggtagcg
     1861 ggtgaaagaa tcccgcgaaa aatgtacagt agtagagaac aaccatagga gatcggacaa
     1921 attccggtcg agaactatgg aggcttcatc aaatggttga caagacccaa attatcagcg
     1981 ctgttacggc agcaatggag caggcgccca agaggaagtt ccaagagagt attgatatta
     2041 ctatcaatct caagcacgtg gacatggcgc agcccaaaaa ccgtattgat gagacgattc
     2101 ttctgccaca ggcaataggc gtcaaaaaga tcgctgtctt gggtaaggga gacattgtgt
     2161 cccaggctcg caacgcaggt gtcgatatca ttatcggtcc tgaagagatt gagcgtctgg
     2221 gtggtgcccc gcgtgaggca cgtaagctcg caggtcagta tgactacttc cttgcagaga
     2281 ctgcggtcat gccacttgtt ggtcgctggc tcggtcagag actcggtccc cgcggcaaaa
     2341 tgccgcagcc gatcccgatg ggtcaggaca ttactccgat cgttgagcgt cttcggaact
     2401 ccgtcaagat ccggtccaag gacagactga acatgtctgt aaagatcgga aacaccgcca
     2461 tgagtgcgga ggcagttgcc gagaacgtcg atgcggtctt aaagagagta ctgggaagac
     2521 tcgaaaacgg cgaattaaac attcgttccg tctacgtcaa gactacgatg ggtcccgctg
     2581 ttaaggtgat gtaaacatgg caatttatac ccaccacctt cccgcgtgga aacgcgaaga
     2641 agttgaatcg attgtggaga acgccggaaa gcacgagctt atcggcctgg tggatgtcta
     2701 cggaattccc gcaagacagt tccagcagat ccgcaggaac ctgcacggca gtgcagttgt
     2761 gaaggttgca agaaacacgc ttgttgagca tgcattcaat gagcttggcg gtcacttcct
     2821 tgaccttaac ggccacgtaa gtgagcactc tgcactgatc ttcgccgacg gaaacccgtt
     2881 caagctgttc aagtcccttg agcagacgaa aacaaagcgt tccgcaaaag caggcgaggt
     2941 cgctcctgag gatatcgttg tccctgcagg accaacttcc ttcaagcccg gaccgatcgt
     3001 cggagaactc cagcaggcag gtatcccggc agctattgac ggcggtaagg tcaaaattaa
     3061 agagaccaag accgttgtaa aagcaggcca ggctatcagc aagaaacagg cagacgttct
     3121 ggcaaagctt gatatcaagc caatgccggt tggtctctca cttctggccg tcgtctacga
     3181 aggcgagatt tatctgccgg atgttcttgg tattgacgaa gaggcataca aggcaaagat
     3241 cactcttgca gcacagcagg tgttcaacct cgcagtcaac gcagccattc cgaccccatg
     3301 tgcacagatc actga
//
LOCUS       DALUAJ010000133         2546 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_24587,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000133 DALUAJ010000000
VERSION     DALUAJ010000133.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24587"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="O0X37_02860"
     CDS             <1..786
                     /locus_tag="O0X37_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kae1-associated kinase Bud32"
                     /protein_id="HJK61898.1"
                     /translation="MCEERGAKFMAPPRVYMGDNGAMIAYTGKVMLEAGSTIPIDQSF
                     VNPGYRSDQVEVTWRSDAGELFAPGQSEITERGAEATVDLTGTDAIKTRHSKGYRVPA
                     LDAHLITERTRAEARCIATARRSGVPVPVIRDLYESAIVMEKLTGDVLKYVISSDYAY
                     AAGESVGKLHKAGIVHGDLTTSNMIWRNGRVYLLDFGLAQMTDEIEPRGVDLHVLFQT
                     LESTTSAPAELKQAFCEGYRSVFPEADAVIKREQEIELRGRYL"
     gene            783..1325
                     /locus_tag="O0X37_02865"
     CDS             783..1325
                     /locus_tag="O0X37_02865"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HJK61899.1"
                     /translation="MITVVTGNKNKAAEVAAFFAGITDVTHIAFDCVEPQADSVEDIA
                     RAKAEQAYAALGVPLIVDDTGLFIDALSGFPGPYAAYVQDTVGNTGILRLMEGKEDRD
                     AHFTTAVAYADATGIQVFSGRVDGVITTEERGNEGFGYDPIFSVGGKTLAEMTLAEKN
                     SLSHRARALAAFRDWYVGSA"
     gene            1388..1531
                     /locus_tag="O0X37_02870"
     CDS             1388..1531
                     /locus_tag="O0X37_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L40e"
                     /protein_id="HJK61900.1"
                     /translation="MRFPEAEARLLNVKVCMKCNARNAIRATTCRKCGSTSLRPKNKE
                     RKA"
     gene            complement(1611..2285)
                     /locus_tag="O0X37_02875"
     CDS             complement(1611..2285)
                     /locus_tag="O0X37_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1917 domain-containing protein"
                     /protein_id="HJK61901.1"
                     /translation="MSEERAPESGDEIMSEELTDLAFGMFTIFMEQQLEKEGTCLYRE
                     IEAGTDLSVRCETLLTAFCEEYPPLAPALAMLGTATDIAGLYAAGEGIIPTKTTKSYW
                     IQQDSPDVHPGATGAEDAGKWLIFLAPEEVDVAWQKVRDATAAGTLGIGAKVSTAKEN
                     EDSRDDRKVIYVFTPDWSDEEDVMRVRNELKNLGFVDRIGYKRNLDTYAGEYREKGKR
                     VTYYSV"
BASE COUNT          636 a          644 c          666 g          600 t
ORIGIN      
        1 atgtgtgaag aacgcggtgc aaagttcatg gcaccacccc gcgtgtacat gggggacaat
       61 ggtgcgatga tcgcctatac cgggaaagtg atgctggaag cgggttcaac catcccgatc
      121 gatcagtcat ttgtaaatcc cggttaccgg tcggatcagg tggaagttac ctggcggagt
      181 gacgcgggag aactgtttgc acccggccag tccgagatta cggaacgcgg tgcagaagcc
      241 acagtggatc taaccggaac agatgcgatc aagacaagac actcgaaagg ataccgtgtc
      301 cctgcgctgg atgcacatct gattactgaa cgtacacgcg cagaggcgcg gtgtattgca
      361 actgcacgac gcagcggagt tccggttccg gtgatccgcg atctctacga aagtgcaatt
      421 gtgatggaaa agctgacagg agatgtgctg aagtatgtta tcagtagtga ttatgcctat
      481 gcagccggag agtccgttgg taaactgcac aaagcaggaa tcgtccatgg agatctgacc
      541 acctctaata tgatctggag aaacggccgc gtgtatctgc tggacttcgg acttgcacag
      601 atgacggatg agatcgagcc gcgcggtgtg gatctgcatg ttttgttcca gacactggag
      661 agtacaacct cagcaccagc tgaactgaaa caggcatttt gtgaagggta ccggtcggtg
      721 ttccccgaag ctgatgcagt tatcaaacgt gaacaggaga ttgaactgcg gggaagatat
      781 ctgtgatcac agttgttacc ggcaacaaaa acaaggctgc tgaggttgcc gcattttttg
      841 ccggaatcac agatgtgaca cacattgcct ttgactgtgt cgagccccag gcagactccg
      901 tggaagacat tgccagggca aaagcagaac aggcatatgc ggcacttggt gttccgttga
      961 tcgtggatga taccggactc ttcatcgatg ctctttccgg atttcccgga ccttatgctg
     1021 catatgtgca ggacaccgtt gggaatacag gcatactgcg gctgatggag ggaaaagagg
     1081 atcgggatgc acactttaca actgcagtag cctatgcaga tgctacggga atccaggtct
     1141 tttccggaag agttgacgga gtcatcacaa ccgaggagcg cggaaacgag gggtttggct
     1201 acgatcccat cttttccgtg ggtggaaaaa cacttgcaga gatgacattg gcagaaaaga
     1261 attccctctc acaccgggca cgtgcccttg cagcgttccg cgactggtat gtcgggtccg
     1321 catgagcgta attgttaata tcattcagca cccacaatat agaactcaat ataggggtta
     1381 ttcatacatg agattcccag aagcagaggc aagacttctt aatgtgaaag tttgcatgaa
     1441 atgcaacgca cgtaacgcta tccgcgcaac cacctgccgc aagtgcggct ccacttcgct
     1501 ccgcccgaag aacaaggaac ggaaagcata agcggctcag tgtgtccgcg cggcctcatg
     1561 tatacagggg cgcggcatta ccgcaccgat ttttttcatt tttaaaaaag ttagacggag
     1621 tagtaggtta cgcgttttcc cttttcgcgg tactcacccg cataggtgtc aaggttgcgt
     1681 ttgtacccga tacgatccac aaatccaagg tttttcagtt cattacgcac gcgcataaca
     1741 tcctcctcat ccgaccagtc gggggtgaaa acatagatga ctttgcggtc atcgcgtgag
     1801 tcctcatttt cctttgccgt gctcaccttt gcgccaatgc ccagcgttcc ggcagcagtt
     1861 gcgtcgcgca ccttctgcca tgcaacatcc acctcttcgg gtgcaagaaa gatcagccac
     1921 tttcccgcat cctctgcacc agttgcacca ggatgaacat ctgggctgtc ctgctgaatc
     1981 cagtaggact tggtagtttt cgttggaata attccctcgc ctgctgcata taacccggca
     2041 atatccgtag ctgttccaag cattgcaaga gcaggtgcaa gcggaggata ctcttcacaa
     2101 aatgcagtga gaagtgtttc acagcgtaca gacagatctg tacctgcttc aatctcacga
     2161 tacaaacagg tcccctcctt ttccaactgc tgttccataa agatggtgaa catcccaaac
     2221 gccagatcgg tcagctcctc agacatgatc tcatccccgg actcaggggc gcgctcctca
     2281 gacatagata ttgaaatatt gtatcagaac cattttagat ttcgcatcca ggacacgaaa
     2341 catattatct cccgcgtctc tattaatatc tacaatgata tggaagcgtg actacggcct
     2401 ccttgcccgc cagatggtgg cgtggctggt tatgggcctt atctacgttg tactcttctc
     2461 agtgatcgcg tggtggctgg caggaaccgg catgttcgga acctcctacc tctatctgat
     2521 cattggaatg gcagcagtaa tggcac
//
LOCUS       DALUAJ010000134         2500 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_24685,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000134 DALUAJ010000000
VERSION     DALUAJ010000134.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24685"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="O0X37_02880"
     CDS             complement(<1..415)
                     /locus_tag="O0X37_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HJK61902.1"
                     /translation="MTKEKLTSSRKMDHLRICSQTDIETGSTLFDDVHLVHCALPECD
                     MDAIDLSVEFLGKRLSSPLFIAAMTGGHPDTAEVNRVLASAAERYGLGIGVGSQRAAL
                     EKPELADSFAIVRETAPHAFVCGNLGAVQLVEHGTE"
     gene            complement(412..1176)
                     /locus_tag="O0X37_02885"
     CDS             complement(412..1176)
                     /locus_tag="O0X37_02885"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HJK61903.1"
                     /translation="MSEITVLKLGGSVVTKKSEAGVIDTARIRELAAQIAPVAKSVPL
                     VIVHGAGSCGHPEAKQYRIQEGVTKSNAEGIAVTHFAVRRLNEEFVSLLRAEGVDAVC
                     MHPFCTSLAKDGRLVYSGEEQIKALLALGVVPVLHGDVVMDTVRGACIVSGDQIVPAM
                     AKALSATRVGVATDVPGVLADDRVVPVITRENVGEIDLGCSSNTDVTGGMRGKVEELL
                     LLADAGIPSHLFHISRVMDFLQDRDHGGTIVQQRKS"
     gene            complement(1169..2041)
                     /gene="mvk"
                     /locus_tag="O0X37_02890"
     CDS             complement(1169..2041)
                     /gene="mvk"
                     /locus_tag="O0X37_02890"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HJK61904.1"
                     /translation="MATWSAPGKVFLFGEHAVVYGKPGIAMAIKPRVMVTVRETRYHQ
                     KPNSPYIAECFRLMDVKGSVYVRSQLPSSSGLGSSAAVTVATLCAINDEFGLDKTRAE
                     IADLAHQVEMSAQNGRASATDTYVSTFGGMVLVRGNEKRRLLPPQNFSLVIGNTLVSH
                     STSEMVGKVAELRKNSPDIANPILDSIAAITMRAMYNLDNQKELGNLMNMNHALLDAL
                     GVGHPVLSKLVLAARSAGAHGAKTTGAGGGGCMYAICSKGARNRVAGAIEGCDARAII
                     TTIDTEGARKEKDE"
     gene            complement(2041..>2500)
                     /gene="amrB"
                     /locus_tag="O0X37_02895"
     CDS             complement(2041..>2500)
                     /gene="amrB"
                     /locus_tag="O0X37_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HJK61905.1"
                     /translation="LLRVQENSLEVQMPFIRYRFPQVKVVPVLLGEQSCAEVHRVADA
                     ILRAIDAVHSDNVVILASGDGSHYVPASVAERDDLTVLAAAAKLDVQKFYEKLIEIQP
                     SMCGYGCIAVMMLVAKQRGAQEAKVIMYQTSGDATGDNREVVGYAAMEVV"
BASE COUNT          606 a          704 c          635 g          555 t
ORIGIN      
        1 actcagtacc atgctcaaca agctggacgg caccgagatt gccacagaca aatgcatgcg
       61 gtgcggtctc acggacgatt gcaaacgagt ctgccagctc cggtttttca agggctgcac
      121 gctgggaacc aacaccgata ccaagtccat agcgttctgc agcactggca aggacacggt
      181 taacctcggc agtgtccggg tgaccgccgg tcatcgccgc aatgaacagc ggtgatgaaa
      241 gacgttttcc gagaaactca accgagagat cgattgcgtc catgtcgcat tcaggcagag
      301 cacagtgaac cagatgcaca tcgtcaaaaa gtgttgatcc tgtctcgatg tcggtctggc
      361 tgcagatacg cagatgatcc atcttgcgtg atgaggtaag tttctctttt gtcatgattt
      421 tcgttgctga acaattgttc cgccgtgatc acggtcttgc aggaagtcca tgacacggga
      481 gatatggaaa aggtgagacg gaataccggc atctgcaagc agcagaagct cctccacctt
      541 tccccgcatt cctccggtta catccgtatt tgaggaacac ccaagatcga tttcaccaac
      601 gttttcgcgg gtgatcaccg gcacaacccg atcgtctgca agaacgcccg gaacatcagt
      661 tgcaacaccg acacgggttg cactgagagc ctttgccatt gcaggcacga tttgatcgcc
      721 tgaaacaata caggctccgc ggacggtgtc cattaccacg tcaccgtgca gaaccggcac
      781 aacgccaagt gcaagaaggg ccttgatctg ttcctcgccg gagtaaacaa gacgtccgtc
      841 ctttgcaaga ctcgtacaga acgggtgcat gcaaaccgcg tcaacacctt cggcacggag
      901 aagagagacg aactcctcgt tcagtcttcg gacggcaaag tgggtaacag caattccttc
      961 cgcgttggat ttagtgacgc cttcctggat acggtactgt tttgcttccg gatgaccgca
     1021 ggatcctgca ccgtgtacaa taacaagcgg gacggatttc gcaaccggtg caatctgtgc
     1081 tgcaagctca cgaattctgg cagtatcaat tacaccagcc tcggattttt ttgtaacaac
     1141 acttccgcca agtttgagta cggtaatttc actcatcttt ttccttccgt gcaccttccg
     1201 tgtcaatcgt ggtgatgatg gcgcgtgcat cgcagccctc aatggcaccg gcgacccggt
     1261 tgcgtgcgcc ctttgaacag atggcataca tacacccgcc tccgccggct ccggtagtct
     1321 ttgctccgtg ggctcccgca gaacgtgctg caagaacaag tttcgagaga accgggtgac
     1381 caacacccaa agcatccagc agggcatggt tcatgttcat gagattgccg agctctttct
     1441 gattatccag attgtacatc gcccgcatgg taatcgccgc aatggagtcg agaatcggat
     1501 tggcaatgtc cggagagttc ttacgaagct cggcaacctt tccgaccatc tccgaggtgc
     1561 tgtgcgagac aagggtgttt ccgataacaa gcgagaagtt ctgcggggga aggagacggc
     1621 gtttttcatt gccgcgaaca agtaccatcc cgccaaacgt ggagacatag gtatcggtcg
     1681 cactggcccg cccgttctgt gcagacatct caacctgatg agcgagatcc gcgatctcgg
     1741 cgcgggtctt atccagaccg aactcatcgt tgattgcgca cagcgttgca acagtaaccg
     1801 ccgcagatga accaagcccg gaggaacttg gcagctggga tctgacgtaa acacttccct
     1861 ttacatccat cagccggaaa cactcggcaa tgtaggggga gttaggcttc tggtgataac
     1921 gcgtttcccg aacggtaacc ataacacggg gcttgattgc cattgcaata ccgggctttc
     1981 cgtagaccac ggcgtgttcg ccgaagagga agactttgcc tggtgcactc cacgttgcca
     2041 tcagacaacc tccattgccg catatccgac aacctcgcgg ttatcaccag ttgcatcgcc
     2101 tgatgtctga tacatgataa ctttcgcttc ctgtgcgcca cgttgctttg caacaagcat
     2161 cataacggca atacagccgt aaccgcacat cgacggctgt atctcgatga gtttttcata
     2221 aaacttctgc acgtcgagtt ttgcagctgc ggcaagaacg gtcagatcat ccctttctgc
     2281 aacggatgcc ggtacataat gtgaaccgtc gccggaagca agaatgacga cattgtcgct
     2341 atgtaccgca tcaattgccc gtaagatggc atctgccacg cggtgaacct ccgcacaaga
     2401 ctgctccccc agcagaaccg gcacaacttt tacctgaggg aaccggtacc ggataaacgg
     2461 catctgcacc tcaagagaat tttcctgaac tctcaaaaga
//
LOCUS       DALUAJ010000135         2807 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_24729,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000135 DALUAJ010000000
VERSION     DALUAJ010000135.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24729"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="O0X37_02900"
     CDS             complement(<1..271)
                     /locus_tag="O0X37_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HJK61906.1"
                     /translation="MEIFPAVDVLRGMCVQLVQGERSTATIYGTPLENAERWINAGAK
                     NLHVVNLDGAFSTSSENVKVIREVVAETDVFVQVGGGIRSIADARG"
     gene            complement(271..1140)
                     /gene="hisG"
                     /locus_tag="O0X37_02905"
     CDS             complement(271..1140)
                     /gene="hisG"
                     /locus_tag="O0X37_02905"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HJK61907.1"
                     /translation="MSDAAKVRLAIPNKGRIAEPINDLMNKSGIHILMSASRQLIAKT
                     VDPQIEVLFVRPIDIPEYVAKGVADIGITGVDMVAERGSDVETLLDLRFGSARLVLAV
                     PEKSPVSTLKDLGGMRVATEFPGICRKYFADAGVDVTLIEVSGACEAAPQLGVADAIV
                     DLTSSGTTLEINKLRIVEELLSSTTNVIANRDALRTKHEKIEEILLAFESVIAAKGQC
                     YLMLNVEKAKLEEIRGLIPGLGGPTIMEIAGSDSVALHAVVPHERVYQLINQLKRNGA
                     RDILVLDITRMVR"
     gene            complement(1144..2361)
                     /locus_tag="O0X37_02910"
     CDS             complement(1144..2361)
                     /locus_tag="O0X37_02910"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HJK61908.1"
                     /translation="MKRNISVKHLSQTPIEKQKVELVERKCIGHPDSLADGVAEAVSR
                     ALCKEYMAECDGGVLHHNTDQGEVVAGESAPAFGGGKIIKPIYFLLTGRATRKFGDKV
                     FATDAIAVNAAREYLKTTIPTFDMDRDIIVDCRMGSGSTDLCDVFNTKKGHTTVPRAN
                     DTSFGVGHAPFSQVEQIILGLDDYIANEFRPKNPMLGYDIKLMGLREINTITITVAAA
                     MVDRYCSDITDYVEMKEKMEESFTKVAKKYTDRKVKVEINTADIIRKKTTSVFLTVNG
                     TSAEMGDDGSVGRGNRCNGLITPNRPMSMEATSGKNPINHIGKIYNLLSTEIAREACQ
                     KVDGIEEMYVRLLSQIGHPIDYPLVASVQCITKRGYDFKEYQSEVEEIVNHRLENITD
                     ITRLVIEGKLKTF"
     gene            complement(2565..>2807)
                     /locus_tag="O0X37_02915"
     CDS             complement(2565..>2807)
                     /locus_tag="O0X37_02915"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HJK61909.1"
                     /translation="LEIFKAVYGHEIKVTAMINKIMETAVAEKDYAAVGELQWFVTEQ
                     VEEEETVRDIVDMLEKAGKSPEALIFIDHQLGSQKE"
BASE COUNT          638 a          707 c          711 g          751 t
ORIGIN      
        1 acccgcgtgc gtctgcaata ctgcggatac cgccgccaac ctgtacaaac acatctgtct
       61 ctgcaacaac ctctcggatt actttcacat tctccgacga tgtcgaaaat gctccgtcaa
      121 gattcaccac atggaggttt ttggcaccgg cattgatcca ccgctcagcg ttctcgagtg
      181 gcgtaccgta aatggttgca gtggaacgct ctccctgcac cagctgtaca cacataccgc
      241 gcagtacatc aactgcaggg aagatctcca tcagcgcacc atccgtgtta tgtcaaggac
      301 aaggatgtcc cgggcaccgt tgcgcttgag ctggttgatc agctgatata cccgctcgtg
      361 gggaacaacc gcgtgcagtg caaccgaatc ggatccggca atctccatga tggtcggtcc
      421 tccaagaccg gggatcagac cacggatttc ctcgagcttt gccttctcaa cattgagcat
      481 cagataacac tggccttttg cagcgatgac actttcaaag gcaaggagaa tctcctcgat
      541 cttctcatgc tttgtgcgaa gtgcatcgcg gtttgcaata acatttgtcg tactggagag
      601 cagttcctca acgatcctga gtttgttaat ctcaagtgtg gtacctgacg aagtaagatc
      661 cacaattgca tcagcaacac cgagctgcgg tgccgcctca caggcgccgc tgacctcgat
      721 gagtgtcaca tcaacaccgg catctgcaaa atattttctg cagataccgg ggaattctgt
      781 tgcaaccctc atacctccaa gatccttcag tgtacttacc ggtgatttct ccggcactgc
      841 aagaaccagc cgggctgagc caaacctgag atcaaggagt gtctcaacat ccgatccgcg
      901 ctcggctacc atgtcaacac cggtgatgcc aatgtcggca acaccttttg caacatactc
      961 ggggatatca atcggccgca cgaacagaac ctcaatctgg ggatcaacgg tctttgcgat
     1021 cagctgacgg gatgcactca tcagaatgtg aatgcctgat ttgttcatca gatcgttgat
     1081 aggctctgct atccgtccct tgttggggat ggcaagccgg actttggctg cgtctgacat
     1141 actttagaag gtctttaatt tgccttcgat aacaaggcgg gtgatgtccg tgatgttttc
     1201 gagacggtgg ttgacgatct cctcaacttc ggactggtac tctttgaagt catagccgcg
     1261 tttggtgatg cactgcacac ttgcaaccag cgggtagtca atcgggtggc cgatctggga
     1321 aagaagtctg acatacatct cttcaattcc atcgaccttc tggcaggctt cacgtgcgat
     1381 ctcggttgag agcaggttgt agatcttacc gatgtggttg attgggttct ttccggaggt
     1441 tgcttccatg gacatcggtc tgttcggcgt gataagaccg ttgcagcggt taccgcgtcc
     1501 aacagatccg tcgtcaccca tctctgcgga tgttccgttg acggtgagga agacagaggt
     1561 ggtcttcttt ctgatgatat cagcggtgtt gatctcaact ttgactttgc gatcggtgta
     1621 cttcttggcg accttggtga aggactcctc catcttctct ttcatctcga catagtcggt
     1681 gatgtcagag cagtagcggt caaccattgc ggctgcaacg gtgatggtga tcgtattgat
     1741 ctcacgcagt cccatgagtt tgatatcgta tccgagcatc gggtttttgg ggcggaactc
     1801 gtttgcgatg tagtcatcga ggccaaggat gatctgttca acctgggaga agggtgcgtg
     1861 accaacacca aatgaggtat cgttcgcccg cgggacggtg gtgtggccct tcttggtgtt
     1921 gaagacatca cagagatcgg tggagccgga acccatgcgg cagtcgacga tgatgtcgcg
     1981 atccatgtca aaggtaggaa tagtggtctt cagatattcc cgtgcagcat taactgcaat
     2041 tgcatcggtt gcaaacactt tgtcaccgaa cttgcgggtt gcacgaccgg tcaggaggaa
     2101 gtagatcggt ttgatgatct ttccaccgcc gaatgccgga gcggactcac ctgcaacgac
     2161 ctcgccctga tcggtgttgt ggtggagaac tcctccgtca cactcagcca tatattcctt
     2221 gcaaagtgcg cgggaaactg cttctgcaac accgtcagca aggctgtccg ggtggccgat
     2281 acatttacgc tcgacaagct cgactttctg tttttcgata ggagtctgag agagatgctt
     2341 aacgctgata tttctcttca cttttgaatc acttctcaat catctgattt ttaagtataa
     2401 aaagatatgt gttgaaaatc atgggatttt gctatgttga cggtctgtgg cgttgtgatt
     2461 ttcagacatc aaaagaaatt ttttcgctgg aatgaataga tccgtatctc ttgtcacatg
     2521 atccggacct caggctaagg atggaaaaaa aaggagagaa aaaattattc tttctgggag
     2581 ccgagctggt ggtcgataaa gatcagggct tccggagact ttcctgcttt ttcgagcatg
     2641 tcaacgatgt ctcttactgt ctcttcttcc tcaacctgtt cggtaacgaa ccactggagt
     2701 tctccaactg ctgcatagtc tttctcggca acagccgttt ccatgatctt gttgatcatt
     2761 gcagtgactt tgatctcgtg tccatagact gctttgaaga tctcaag
//
LOCUS       DALUAJ010000136         2317 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_24755,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000136 DALUAJ010000000
VERSION     DALUAJ010000136.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24755"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="O0X37_02920"
     CDS             complement(<1..683)
                     /locus_tag="O0X37_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2099 family protein"
                     /protein_id="HJK61910.1"
                     /translation="MVDEHVIEAVGMTRVVIRDGTVVETGEPKIRSCPLAKRFANPVD
                     PITSEAVAANISSRIQRFGMCTRDRMVLDKETFVLFGASELMNTGLRTGTLDAAVIAC
                     DGAGTVIVRSPEMVQGIGGRMSGLVSTTPYPEVIARIEAEGGVVVNPETAEMDALAGL
                     AKAKEMGWTKTAVTIAGFQHELAEEIREKYPEALIIVVHTSAVSSLENAMRLARAADL
                     VFSCASKYVR"
     gene            743..1474
                     /locus_tag="O0X37_02925"
     CDS             743..1474
                     /locus_tag="O0X37_02925"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HJK61911.1"
                     /translation="MGRYWELDACRGVAILLMIGYHVLFQISFFDPALLPWFDPYVFT
                     GAPIAFLFVTIAGISLILFTAKEASLPKTAQKMFVRGVYILFFAAVITIASWLLFPSE
                     VVVFGILHLIGCSTILAIPFVVLKIRGWIVFGFGVIIIALSPFLAYVRGPALLIPLGI
                     TPVGFATLDYEPLIPWFGVLLIGVALGSVLYRGGVRCKVLEKLGEMPKVASPLAFLGR
                     HSLLIYLIHNPIIFAVLWIAGVIPW"
     gene            1544..>2317
                     /locus_tag="O0X37_02930"
     CDS             1544..>2317
                     /locus_tag="O0X37_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 14 protein"
                     /protein_id="HJK61912.1"
                     /translation="MTAGFFDRFTAKKPWIVESVPPPATGHGAGMSVPEYKSKPYFIV
                     ASVEMGNTTTKCILTGTDLEDGRSYVINKTVSMSRDVRPPKPGEEVFGTTLDGTELTR
                     ESVTELVRDTLIKCHHDADLSIKNDLDFVVRSTGVVAAMDSPDQVGDFIVALANGCLE
                     AGVPPKKMTPPMGKDNLPPKLREFSFADRLVFTGAVAGVTPPAGASGVEMVANEMEGE
                     LAMAGIKEGAKWTPVDFRNPCISMDFGTTLDGRITSDVDP"
BASE COUNT          527 a          574 c          566 g          650 t
ORIGIN      
        1 cgtacatact ttgatgcaca ggagaagaca aggtctgctg cccgcgccag ccgcattgca
       61 ttttcaagcg atgataccgc ggatgtatgc accacaatga tcagtgcttc aggatatttc
      121 tctcggatct cctcagcaag ttcgtgctgg aacccggcaa ttgttaccgc cgtttttgtc
      181 catcccatct cttttgcctt ggcaaggccc gcaagagcat ccatttcggc ggtctctgga
      241 ttaaccacaa ctcccccctc tgcctcaatg cgtgcaatga cctctgggta gggagtggtg
      301 gaaacaaggc cggacatcct gccgccgata ccctgtacca tttcgggtga tcggacaatt
      361 acagttcccg caccatcgca tgcgattact gcggcatcca gtgtacctgt ccgaagaccc
      421 gtgttcatca gttcggatgc accaaacagt acgaatgtct ctttgtcaag aaccattctg
      481 tcccgtgtac acataccaaa cctctgaatt cgggatgaga tatttgcagc aactgcttca
      541 cttgtaatgg gatccaccgg attggcgaat cgttttgcaa gcggacagga tctaatcttt
      601 ggttcgcccg tttcaacgac tgttccgtcg cgaattacta cacgggtcat accgactgct
      661 tcaataacat gttcgtcaac catgggaatc acataactga tgtatagata tggttcggca
      721 aaaaacataa gatcactaca ctatgggacg atactgggaa ctcgatgcat gccgcggtgt
      781 ggcaatcctt ctgatgatag gttatcatgt actgtttcag atcagttttt ttgatccggc
      841 cctgctcccg tggtttgatc cctatgtttt cactggtgcc ccgattgcat ttctgtttgt
      901 gacaatcgct ggtatctctc tcattctctt tacggcaaaa gaggccagtc ttccgaagac
      961 tgcacaaaaa atgtttgttc gaggtgtcta cattctcttc tttgccgcag tgatcacaat
     1021 cgcctcatgg ctgctctttc catctgaagt ggtagtgttt gggattctgc atctgatcgg
     1081 atgttcaacg atccttgcca taccttttgt tgtgttaaag atacgtggat ggatagtctt
     1141 cggctttggt gtaatcatca ttgccctctc accattcctt gcctatgtta gaggtccggc
     1201 actgctgatt ccgttaggca taactcctgt cgggtttgca acactggact atgaaccgct
     1261 tattccctgg tttggagttc tgttaatcgg tgttgccctc ggttctgttc tctacagagg
     1321 tggtgtccgc tgcaaggtac tggaaaagct tggtgaaatg ccaaaggttg cctcgcctct
     1381 tgcatttctt ggacgtcatt ccctccttat ttacctgatc cataacccga tcatctttgc
     1441 agttctctgg attgcaggag ttataccctg gtaaccgggg tggagttcat tcgctgcagc
     1501 gatggaaatg tatttatgaa tcagcgatag aactctctgt aatatgacgg caggtttttt
     1561 tgaccgattt accgcgaaga aaccgtggat cgtggagagc gtccctccac cggctacagg
     1621 tcacggagcg ggaatgagtg ttcctgaata caaatcaaag ccgtatttca ttgttgcatc
     1681 tgttgagatg ggaaatacga caaccaaatg cattctcacc ggtactgatc tggaagatgg
     1741 tcggtcgtat gttatcaaca aaacagtctc tatgagccgt gacgttcgcc ctcccaaacc
     1801 aggtgaagag gtttttggga ccactcttga cggaacggag cttacccgcg aatcggtgac
     1861 ggaactggtt cgggatacct taatcaaatg tcatcacgat gccgatctga gcatcaaaaa
     1921 cgatctggat tttgttgtca gaagtactgg tgttgttgct gcaatggact ctccggatca
     1981 ggtcggggat ttcattgttg cccttgcaaa cggctgcctt gaggcaggtg tcccgcccaa
     2041 gaagatgact ccgccaatgg gaaaagataa ccttcccccc aaacttcgtg agttcagttt
     2101 tgccgatcgt ctggtcttta ccggagctgt agctggagtt accccgcctg ccggagcatc
     2161 tggagtggag atggtcgcaa acgagatgga aggggaactc gccatggcag gtatcaaaga
     2221 aggggcgaag tggacacctg ttgatttccg gaatccctgt atttcaatgg attttggtac
     2281 tacacttgac ggaagaatca ccagcgatgt tgatccc
//
LOCUS       DALUAJ010000137         2390 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_24807,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000137 DALUAJ010000000
VERSION     DALUAJ010000137.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24807"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="O0X37_02935"
     CDS             <1..820
                     /locus_tag="O0X37_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HJK61913.1"
                     /translation="SGNLRVAAAVGPFDMNRAMMLAEAGADAIIVDCAHGHNMHVVQG
                     VKDIKGSVSCDVIAGNIATAAAARELVDFVDGIKVGIGPGSICTTRIVAGVGVPQVSA
                     IANVCDIADPAGVPVIADGGVKYSGDVAKAIVAGASSVMMGSMFAGTDEAPGKIIVVK
                     GRRYKQYRGMGSLGVMTSGQSSDRYFQKKGIGATKYVPEGVEGATPYVGSVTDVIYQT
                     IGGLKSAMGYTGSKDIESMRKNARFVRITAAGLKESHPHDILITDEAPNYRMGL"
     gene            complement(916..1434)
                     /locus_tag="O0X37_02940"
     CDS             complement(916..1434)
                     /locus_tag="O0X37_02940"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HJK61914.1"
                     /translation="MKEYLITLTFLLAVALFAVPATAAIVSDFTYTVDPSDPLTIHFT
                     EKSTGGAGNWFWMFNDGGEVETSTERNPTHTFSGEGTYLVSCVAQSSDYSEENTMRKQ
                     LTITLGGVTDDSGGSGSSSGGSSSSGGNSGGGISLPDISLGGISIPNPMDIIGEYIKL
                     IRVMIIPSNYNI"
     gene            complement(1444..>2390)
                     /locus_tag="O0X37_02945"
     CDS             complement(1444..>2390)
                     /locus_tag="O0X37_02945"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HJK61915.1"
                     /translation="YVPLSVLFTATATNSPTEYQWTFGDGTSETETTTNTITHEFKKT
                     GTYTVNVTAKNTNGTSEKKSITIVVNANASNYKVAIDATPVKGIAPLTVKFKLNTTIP
                     DEDISEVDWDFGVDNKSWISKNDPAYKSPSWTYEEDGNYKVTLTVKSALTNEEYQADV
                     MISVSDIVASFTASTTSGTAPLEVKFTDTSTGATAWTWNIYKTDGGSRTLQKELTDRN
                     ITYTFQNTGTYEVELIAKKGSNTATESKTITVTARATTVPTTVKTTAPTTATTTVSAV
                     KSASLSADDNPIPNPLDIIEELIRLLKVMLVPENYSLA"
BASE COUNT          511 a          481 c          661 g          737 t
ORIGIN      
        1 gagcgggaat ctccgtgtgg ctgcagctgt cggtccgttt gatatgaacc gtgcgatgat
       61 gcttgctgag gctggtgctg atgcaattat tgtggactgt gcccacggtc acaacatgca
      121 tgttgtgcag ggtgtaaaag acatcaaagg ttccgtctcc tgcgatgtga ttgcaggaaa
      181 catcgcaaca gctgcggctg cccgtgaact ggtggacttt gttgacggta tcaaggttgg
      241 tattggtcct ggctcgattt gtacaacccg cattgttgca ggtgttggtg ttccgcaggt
      301 gtcggctatt gcaaatgtct gtgatattgc ggatcctgct ggtgttccgg tgattgcgga
      361 tggtggtgtg aagtacagcg gtgatgtggc gaaggcaatt gttgctggtg catcgtcggt
      421 gatgatgggc agtatgtttg ccggaacgga tgaggccccc ggtaagatta tcgtggtaaa
      481 gggccgccgc tataagcagt accgcggtat gggctctctt ggtgtgatga cttccggtca
      541 gtcaagcgac cggtacttcc agaagaaggg aatcggtgca acgaagtatg tgccggaggg
      601 tgttgagggt gcgacaccat atgtggggtc tgtaacggat gttatctatc agactatcgg
      661 cggtctgaag tctgcgatgg gttacaccgg cagcaaggac attgagtcaa tgcgcaagaa
      721 tgcacgcttt gtgcggatta ctgctgctgg tctgaaggaa agtcacccgc atgatatttt
      781 aattacggac gaggccccga actaccggat gggtctctga tttttttcat ttttttgttt
      841 gtgaatttct ctgtgatcca tttccctatg cttgcttatc tgcacgtgtg gagtgatgga
      901 tagaaaaaag agtgtttaga tgttgtagtt gcttgggatg atcattacac ggatgagttt
      961 gatgtactca ccaatgatgt ccatcgggtt gggaatgctg atgccgccca atgagatgtc
     1021 cgggagactg attccgccac ctgaattccc accgctggaa ctgcttccac cagaggagct
     1081 tccagatcca ccactgtcat cagttacacc gccgagcgtg atagtgagtt gttttctcat
     1141 ggtgttctct tcactatagt cagatgactg tgctacacag gaaaccagat atgtcccctc
     1201 tccgctgaag gtatgtgtgg gattgcgctc ggttgaggtc tccacctccc ccccgtcatt
     1261 gaacatccag aaccagttac ctgcaccccc agtggacttt tcggtgaaat gaattgttag
     1321 agggtcagag ggatcaacgg tatacgtaaa gtctgagacg attgcagcag tggcaggtac
     1381 tgcaaatagt gcaactgcaa ggaggaaggt gagtgttatc agatattctt tcatatgttt
     1441 acctcaggca agtgagtaat tctctggaac cagcatgacc ttcagcaggc gtatgagttc
     1501 ctcaatgata tccagaggat tggggatggg attgtcatca gcagataggg atgctgattt
     1561 cacggcagag accgttgttg tagctgttgt tggtgctgtt gtttttactg tggttggaac
     1621 tgttgttgct cttgccgtta cggtaattgt tttggattcg gtggctgtgt tggagccttt
     1681 ctttgcgata agctctactt cataggttcc cgtattctgg aacgtatagg tgatgtttcg
     1741 atctgtcaat tctttctgca gggttcgaga cccgccgtcg gttttgtaga tgttccatgt
     1801 ccaggcggtg gcacctgttg aggtgtcagt gaatttaacc tctaggggag ctgtgccgga
     1861 tgttgttgat gcggtaaagg aagcaacaat gtcggataca gatatcataa catctgcttg
     1921 atattcctca tttgtcaggg ctgatttcac agtgagtgtt accttataat taccatcttc
     1981 ttcgtatgtc cacgaggggg atttgtatgc tggatcattt tttgatatcc atgatttatt
     2041 gtccactcca aaatcccaat caacctctga gatatcttca tcagggattg tggtattcaa
     2101 cttgaactta actgtcagcg gggctatccc tttcactggt gttgcatcga tggcaacttt
     2161 gtaattggag gcatttgcat ttactacaat cgtaattgat ttcttttctg atgtcccgtt
     2221 tgtatttttt gcggtgacat tcacagtata cgttcctgtt ttcttgaatt cgtgggtaat
     2281 ggtattggtt gtggtttctg tctctgatgt cccgtcacca aatgtccatt gatattctgt
     2341 tggtgagttt gttgcagttg cagtgaataa tacagatagt ggtacatatc
//
LOCUS       DALUAJ010000138         6042 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_24834,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000138 DALUAJ010000000
VERSION     DALUAJ010000138.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24834"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="O0X37_02950"
     CDS             complement(<1..885)
                     /locus_tag="O0X37_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HJK61916.1"
                     /translation="MTDYEETYSTFSIAVPEYYNFGFDVVDAWAKKDRNKLAMIWTNQ
                     KGEEKYFTFRHMMNLSNQIANMMFKQNIGKGDRVMIMLPRVPEWWTFAIAAIKIGAVF
                     CPSPCILTPHDLKYRINQGKFKMIVTDMENSWKIEEILSECPTLQIKFLTDGGLPGWI
                     NYQTELVHPAPASTKLIPLARSVRTKATDPMLIFFSSGTTGDPKMVLHTHAYPLGHIV
                     TGRFWYDLTENDLHFTVADTGWGKSSWGKFYGPWMQGACVFVYDYRGKFNATELLPLI
                     EHYEITTFCAPPTIYRMLI"
     gene            1133..4075
                     /locus_tag="O0X37_02955"
     CDS             1133..4075
                     /locus_tag="O0X37_02955"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HJK61917.1"
                     /translation="MSVSHTIRYALFGELLTETFAAYQDAASRDPFGTWFVLPTRNLA
                     DVILRHPIAKERLILPDHMLTARDFAQYICRETDPDLYILSSGEQDLLIRRILTSPDL
                     RKSTASLFGEVTHTTCSSLRTLITGLEQNLTDLSVIHSSTRKIETLSAIHQEYTRILK
                     EEHLADKETVYETAVVALGSGKIPFRTVFIYGMYSLQPNEQAFINAIIATAESVHEVR
                     PAVNNHAIFPGFTGEPIHGDPEKAAFAEGLFAGHEHRIPASLPVNLTTVIYPDTTAEV
                     SSVLDQVSGLFEDGVGAEDILILSPAVKETVRLIDDIIDDFSIRNPDGTRTRISYTAA
                     TARPISNYPETAALLAVLKAPVADYPLATLLQLLTLPRIRKKFGIHTSQLFSLSQTLG
                     ITRGKSNWITIADQRIPFLLQKIEDAGTDSQSAKFYQVQIDELQKLQQGLISLIEEIT
                     PKNQKKQSVRQHCRDCLDLFIKLGLLEPVECEMPTEQQLRQKAFHDLISGLAASPVAQ
                     TISLTHAAFWDTLSELCTSTPAPLSPRTAKHTITVTGLRESALTSANHVFILGLSDKF
                     IPRIQFTLPYLTVKETAKIKNTSFETDILREKYYFAAACLVAGESLHLSCAERDSSGA
                     LSPSSFFTRFPEQYKTQAPDFLSHSQTDNQMTAGLAVLNNTIADHPDLFGLPPYPPRK
                     QETLQVCFGDLPDLAEQFSSAYNDQTTFAPTMLETYASCPFKWYLEKPLRLIPPVSSH
                     SEERSMLGLVLHRIMERFFTEWTDPITLENKEDAKLCLTEIAAEEFDRPGFTSPEWEA
                     EKNLYLTRRLFGVIDTELDPKLIHQPAAFRRLEQPLADTTAVSVPDAAPLRISGRPDR
                     IDFFPDNSFTVYDYKSGDISTAKYNVKGLEAGISLQLPLYVAAVEAEDSGVSGAAAFY
                     QIHPRGVKLQRFSKDVLYKHLPDILSYCKSYRSGMQNGVCSPNPNSSDCSYCPWRFVC
                     RVTTGGEE"
     gene            4075..>6042
                     /locus_tag="O0X37_02960"
     CDS             4075..>6042
                     /locus_tag="O0X37_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HJK61918.1"
                     /translation="MPETLTPSQKRALDVTKSTCVTAGAGTGKTFILSQRYRSVLLQN
                     HANKQFSPANILALTFTEKAAAEMRERIAADLSEDIRACSDPTVQQGLLRMQDEFYRA
                     SVTTFHSFASSILREFAVEAGLDPSFGIMDAGSQSDLVHTTVTALLTHPPEDLRQDVL
                     CLLQYLKSDTLSSLLAYALDDWRKAEPWFQQLEENPDAVRELWETTSADFLVLVRDNL
                     VQNQEICQTIGEISAVVGETKYGETVTENWQKLKAAESTAEILAALRGLKGANKGNIC
                     LTGIDETIFKDPINEILNMDLDPGKPPFDEISLVLISFGRVLRAAAKSIDAEKQRLNL
                     LDFDDLIYRAVRLITENPAVLSELWERYRYILVDEVQDNDPRLTDLILRLAGSPAASD
                     RLFIVGDLKQSIYRFRGADIRKNSALFDAFPATSRVSLDVSFRTVPEIVHLVNTVFSQ
                     VFTGAARSYDVSYDALSANRTQDTGSVQLLCRKKEQGEDTSTARMRESEDIAAWIAGS
                     VRNQTLLIKTKDGVSRPAEYGDIAVLLNTRTPLPQLTAALQKFSVPYLIYKSGMLYQQ
                     QETYDFSNLLEVIINPENDAALYGLLRSPWFGISDADLCRLRTGRFSLWKDVRDSPLA
                     ESVVQTIRHWQKLSLQQPIGRFLRTVLRESGI"
BASE COUNT         1456 a         1680 c         1370 g         1536 t
ORIGIN      
        1 aatcagcatc cggtaaatcg tcggcggagc acagaacgtc gtgatctcgt agtgttcaat
       61 cagcggcaga agttcggttg cattgaactt tccgcggtaa tcgtacacaa acacacaggc
      121 accctgcatc caggggccgt agaacttacc ccaggaggac tttccccatc cggtgtctgc
      181 gaccgtgaaa tgcagatcgt tttccgtcag atcataccag aaccttccgg ttacaatgtg
      241 tcccagagga taagcgtgcg tatgcagcac catctttgga tcaccggtcg ttcccgacga
      301 gaagaagatc agcatcggat cggttgcctt tgtgcggaca ctgcgtgcca gcggaatcag
      361 cttcgttgac gcaggagccg ggtgaacgag ttctgtctgg tagttaatcc agccgggcag
      421 tccaccgtct gtcaggaatt taatctgcag agtcggacac tcggacagga tctcctcaat
      481 cttccaggag ttctccatgt ccgttacgat catcttgaac ttgccctggt taatacggta
      541 cttcagatcg tgcggagtaa gaatgcaggg ggacggacag aaaactgcac caatcttaat
      601 ggcggcaatc gcaaaggtcc accattccgg aacccgcggc agcataatca ttacccggtc
      661 gcccttgccg atgttctgct tgaacatcat atttgcaatc tgattggaca gattcatcat
      721 gtggcggaag gtaaagtact tctcctcccc tttctgattc gtccagatca tggcgagctt
      781 gtttctgtct ttcttcgccc atgcatcgac tacgtcgaat ccgaagttgt agtactccgg
      841 cacggcaata gaaaacgtgg aataggtttc ctcgtaatct gtcatattgt gtgatgccgt
      901 ttcggtcatg cgctcacatg ttgcacgcga cagatgataa attctctcgt gatggatagg
      961 tgatcttgcc cggtattctg gtggtgctgg ctctcctgtc cccaggcaaa ctaccatata
     1021 gaagtacaat ataaataaca agagtcaaat cagttagtga aacgtaccgg taactacaca
     1081 aatggactgt tatagtccgg caacctcctg aatatcatca tctttcacaa ccatgtctgt
     1141 atcacacact atccgttatg ctctttttgg agagcttctt actgaaacct ttgctgccta
     1201 tcaggacgct gcatcccgtg acccgttcgg tacatggttt gtgcttccca cgcggaatct
     1261 tgctgatgtg atcctccgcc atccaatagc caaagaacga ctgatcctcc cggatcacat
     1321 gcttaccgca agagattttg cccagtacat ctgtcgcgaa acagatccgg atctctacat
     1381 tcttagctct ggagaacagg accttctcat ccgcagaata ctcacaagcc ctgatcttcg
     1441 caaaagtacc gcctctcttt ttggcgaggt cacccacacc acttgcagca gtctccggac
     1501 actcatcacc ggtcttgagc aaaatctgac cgatctctct gttattcact cttcaacccg
     1561 aaagatagaa acactctctg caatccacca agaatacacc agaatactaa aggaagaaca
     1621 tcttgcagat aaagagactg tctacgaaac tgccgtggtt gcccttggtt caggaaaaat
     1681 tcctttcagg acggtcttta tctacgggat gtactccctg cagcccaacg aacaagcttt
     1741 catcaacgct attattgcta cagctgagag tgtgcatgaa gtgcgtcccg cagtgaataa
     1801 ccatgctatt ttcccgggat ttactggaga accaattcac ggtgacccag agaaggctgc
     1861 gtttgctgaa ggattgtttg ctggacacga acaccgtatt cctgcatctc ttccggtcaa
     1921 tcttaccaca gtcatctatc cggacaccac tgcagaagtt tcctcggttc ttgatcaggt
     1981 atccggtctt tttgaggatg gtgtcggtgc agaggatatc ctcatcctca gcccagccgt
     2041 aaaggagact gtccggctga tcgatgatat cattgatgac tttagtatcc ggaatccaga
     2101 cggtaccaga acacgcatct cgtacaccgc tgcaaccgca cggccgatta gtaactatcc
     2161 ggaaacggca gcacttctcg cagttctcaa agcccctgta gcggactatc ccctggccac
     2221 tcttctccag ctccttacgc ttccgcggat ccggaaaaag tttggcatcc acacatcaca
     2281 actgttctct ctttcacaga cgctcggtat cactcgcgga aaatccaact ggatcactat
     2341 tgctgaccaa cggattccgt tccttctcca aaagatcgag gacgcaggta cggacagtca
     2401 gtctgccaaa ttctatcagg tacagataga tgaacttcag aaactccaac agggtcttat
     2461 cagtcttatt gaggaaatca ctccaaaaaa tcagaaaaaa cagtctgtta ggcagcactg
     2521 tagagactgt cttgatcttt ttattaagct tggcctgctt gagcctgttg agtgtgagat
     2581 gccaacagaa cagcagctca ggcagaaagc gttccatgat ctcatctcgg gtcttgctgc
     2641 atcgccggtt gcacaaacca tcagtctcac tcatgccgcg ttctgggaca ccctttccga
     2701 actgtgcacc agcactcctg cccccctctc tcctcgtacc gctaaacaca ccatcaccgt
     2761 taccggactc agagagtcgg ccctcacctc tgcaaatcac gtctttattc tcggtctttc
     2821 cgacaagttc atccctcgca ttcaatttac tcttccgtat ctgaccgtca aagagactgc
     2881 aaaaatcaaa aatacctcct ttgaaaccga tatcctccgt gaaaagtact actttgccgc
     2941 agcctgcctt gtagctggag agagcctgca tctctcctgt gctgaacgcg actcctccgg
     3001 ggcccttagc ccttcctcat tttttacccg gttcccggaa cagtacaaaa cccaagcacc
     3061 agatttcctt tcccattccc aaactgataa tcagatgaca gcagggcttg ctgttttgaa
     3121 taataccatc gctgaccatc ccgatctgtt tggtcttccc ccgtatcctc cgcggaaaca
     3181 ggagaccttg caggtttgtt ttggtgatct tcctgacctt gccgaacagt tttcttctgc
     3241 ctacaatgat cagaccactt ttgctcctac catgcttgag acctatgcct cctgtccgtt
     3301 taaatggtac ctcgaaaaac ccctccgtct catcccgcca gtgtcttctc actcggagga
     3361 aagaagcatg cttggtcttg ttcttcatcg aatcatggaa cggttcttta ctgaatggac
     3421 agatccgatc accctggaaa ataaagaaga tgcaaagcta tgccttacag agatcgcagc
     3481 cgaggagttt gatcgtcctg gctttaccag tcctgagtgg gaggctgaaa agaatctcta
     3541 cctcacccgc cggctctttg gcgtaataga tacggaacta gatccgaaac ttattcacca
     3601 accggccgca ttccgccgtc ttgaacaacc tcttgcagac accactgcag tatctgtgcc
     3661 ggatgccgcc cctcttcgta tttcaggacg gcctgaccgc atcgattttt tcccggacaa
     3721 cagcttcacc gtctatgact acaaatcagg tgacatcagc acagcgaagt acaatgtcaa
     3781 gggcctcgaa gctggcatct cgcttcagct tccgctctac gttgctgcgg ttgaggcgga
     3841 ggattccggt gtatccggtg ctgccgcatt ttatcagatc catccacgcg gagtaaaact
     3901 gcagaggttt tcgaaagatg tactttacaa gcacctcccg gatatcctct cctactgcaa
     3961 atcctaccgg tccgggatgc agaacggcgt ttgttctcca aatccgaaca gttctgactg
     4021 ttcatactgt ccatggcgat ttgtctgccg cgtaactacg ggaggtgagg agtgatgccc
     4081 gaaacactta ccccttctca gaaacgggca cttgatgtta ccaaaagtac ttgtgtcact
     4141 gccggagcag gtaccggcaa gaccttcatt cttagccagc gctatcgttc tgttcttttg
     4201 cagaatcatg caaataaaca atttagtccg gcaaatattc ttgctcttac ctttaccgag
     4261 aaagccgcag ccgagatgcg ggaacgcatc gcagccgatc tttcggaaga tatccgtgcg
     4321 tgcagtgatc caactgtgca gcagggcctt ttgcggatgc aggatgagtt ctaccgtgca
     4381 tccgtcacca ccttccacag ttttgcatcc tccattctca gggaatttgc cgtcgaagca
     4441 ggtcttgatc cgtcgtttgg tatcatggat gcaggatcac aatcggatct tgttcataca
     4501 acagtaactg ctcttctcac tcatccgcca gaggatctcc ggcaggacgt tctctgtctt
     4561 ctgcagtatc tgaagagcga tactctcagt tctctccttg catatgcatt ggatgactgg
     4621 cggaaagcgg aaccctggtt ccagcagctg gaggagaacc ctgatgccgt ccgggaactc
     4681 tgggagacaa ctagtgcaga tttcttggtg ttagtccggg ataatcttgt tcaaaatcag
     4741 gaaatatgtc agacaatcgg tgagatctcc gcggttgtcg gggagaccaa atacggtgaa
     4801 accgtcacgg aaaactggca aaaactcaag gccgccgaaa gtaccgcgga gattcttgca
     4861 gctctgcggg gtttgaaagg tgccaacaaa ggtaatatct gtcttaccgg aattgatgag
     4921 acgatcttca aagacccaat caatgagatt ctgaatatgg atctggatcc tggaaaacct
     4981 ccgtttgatg agatcagttt ggttctcatc tcctttggac gggtgctcag ggctgctgcg
     5041 aagagcattg atgcagaaaa acagcggttg aaccttcttg attttgacga tcttatctac
     5101 cgggcagtcc ggctcatcac cgagaatcct gcagtcctaa gcgagctttg ggaacgatac
     5161 cgatacatcc ttgttgatga agtacaggac aatgatcccc gtctcacgga tctgatcctc
     5221 cgtcttgccg gttctccggc tgcatccgac cgccttttca tcgtcggcga tctcaagcag
     5281 tccatctacc ggttccgcgg agccgacatc cggaaaaatt ccgcactctt tgatgcattc
     5341 cccgcgacat cccgtgtcag tcttgatgta agtttccgga cggttccgga gatcgttcat
     5401 ctcgttaaca cggtattctc ccaggtcttc accggagctg cacgatccta tgatgtctcg
     5461 tatgatgctc tatctgcaaa ccgtacccag gacacgggtt ctgtccagct cctttgcagg
     5521 aaaaaagaac agggggagga cacaagcacc gcccgtatgc gggaaagcga agacattgca
     5581 gcctggattg ccggatccgt ccgtaaccag accctcctca tcaagacaaa agacggtgta
     5641 agccgtcccg cagagtacgg tgacattgcc gttctcctta acacccgaac accgctcccc
     5701 cagctcaccg cagccctgca aaagttctcg gtaccgtatc tgatctacaa aagcggcatg
     5761 ctctatcagc agcaggagac atatgacttt tccaaccttc ttgaggttat catcaatccg
     5821 gaaaatgatg cagcattgta tggtcttctc cggtcaccct ggtttgggat cagcgatgcc
     5881 gatctctgcc gtctgcggac cggtcgtttc tccttatgga aggatgtccg tgacagtcct
     5941 ctggctgagt ccgttgtgca gaccatccgg cactggcaaa aactctcact tcaacagccc
     6001 attggaaggt ttttgcgaac tgttctcaga gagtccggca tc
//
LOCUS       DALUAJ010000139         2184 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_24972,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000139 DALUAJ010000000
VERSION     DALUAJ010000139.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24972"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /gene="argF"
                     /locus_tag="O0X37_02965"
     CDS             <1..653
                     /gene="argF"
                     /locus_tag="O0X37_02965"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HJK61919.1"
                     /translation="ARVMSRYVHGAIMRTYKHETITEFAKYASIPVINALSDKEHPCQ
                     ILADSLTLKEKFGDLNGLRVAWIGDGNNVCNSLIMATVQTGMEIAVATPKGYEPDPAA
                     IRFARENGGKIVEYDDPKKAVEDAHAIYTDTWISMGEEDIKDTKLKDFNGFCLDMPLL
                     KRAAPDAIVLHCLPAHRGEEITDEVIDSMQSGVWDQAENRLHAQKAVLVRLMGQNL"
     gene            complement(654..1322)
                     /locus_tag="O0X37_02970"
     CDS             complement(654..1322)
                     /locus_tag="O0X37_02970"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HJK61920.1"
                     /translation="MDEYSEVVLWLNDHANSHSAEGMRRFGISGGKILGISVVNLRRY
                     AKTLGKDQGLSVFLWNTGIHEARILASMTADPACADDALLELWVLDLDSRDVCDQVCK
                     NFFAHCSGTQERAVRWCSREEEFVRRAGFVLICEMAIHHKDMPDEAFLPYLDLIRRYS
                     TDDRNFVKKAVNWALREIGKRRHGISRECLDLSDELAVSENPTARWIGRNAGQELRDK
                     IKKK"
     gene            1363..1920
                     /locus_tag="O0X37_02975"
     CDS             1363..1920
                     /locus_tag="O0X37_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011450100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HJK61921.1"
                     /translation="MTDQEIFSDLSRIGKRLFIEHLVGGNFGNMSARYADGYFITRTG
                     SYLDADPAQVVLMPLNGRVTPGASSEWRVHTAIYNEAEQHAAIVHAHPPYAVALSLLV
                     ENEILTIDSEGKMLAPSIAVVDGAPGTQELADAVAAGLSCANVVIARGHGTFAAGKDL
                     DQAYLYTSLAEHCCKVLHYTKLYRQ"
     gene            complement(1944..>2184)
                     /locus_tag="O0X37_02980"
     CDS             complement(1944..>2184)
                     /locus_tag="O0X37_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61922.1"
                     /translation="YQKEKLSFAFPALCDALKNKYGDKVSVELVPLDKEIPDRIKDIV
                     RADHPPLPIILINGKCVPVGAVSVPRISESIDPLL"
BASE COUNT          584 a          555 c          540 g          505 t
ORIGIN      
        1 ccgcacgggt gatgtcccga tatgtccatg gtgcaattat gcggacatac aaacatgaga
       61 cgattacgga gtttgcaaag tatgcatcca ttccggtgat caatgcactg tccgacaagg
      121 aacacccgtg ccagattctt gcagattctc ttactctgaa ggagaagttt ggggatctca
      181 acggtctgcg ggttgcctgg atcggtgacg gcaacaatgt ctgtaactca ctgattatgg
      241 caacggtcca aacgggaatg gaaatagcgg ttgcaacacc gaagggttac gaaccggatc
      301 cggctgcaat ccggtttgca cgggagaacg gcggaaagat tgtggagtat gatgatccaa
      361 agaaggcagt agaggacgca cacgctatct acacggacac atggatttcc atgggcgagg
      421 aggacatcaa ggacaccaag ctgaaggact tcaacggatt ctgtcttgat atgccgctcc
      481 tcaaacgcgc tgctccggac gcaattgttc tgcattgttt gcctgcccat cgcggagagg
      541 agattacaga cgaggttatc gactccatgc agagcggagt ctgggatcag gcagagaacc
      601 gtctgcatgc acaaaaagca gttcttgtcc ggctgatggg acagaatctg taatcatttt
      661 ttctttattt tatcccggag ttcctgcccg gcatttctac caatccatcg tgcagtggga
      721 ttctcagaga ccgccagttc atcagaaaga tccagacact ctctgctgat accgtgtctg
      781 cgttttccaa tttcacgcag tgcccagttg accgcttttt ttacaaaatt ccgatcgtcg
      841 gttgagtacc ggcgaatcag atccagatac ggaagaaaag cttcgtccgg catatctttg
      901 tggtggatgg ccatctcgca gatgagaaca aaacctgcac gtcgaacgaa ctcttcctca
      961 cggctgcacc agcgtacggc ccgttcctga gttccgctgc agtgcgcaaa gaagttcttg
     1021 cagacctgat cacagacatc ccgtgaatcg agatccaaaa cccataactc aagaagggca
     1081 tcatcagcac atgccggatc agctgtcatg gacgcaagaa ttcttgcttc gtgaatccct
     1141 gtattccaga ggaaaacaga aagtccctga tcttttccaa gtgttttggc atacctccgc
     1201 aggtttacaa ccgaaattcc aagaatcttc ccgccggaga taccaaagcg tctcataccc
     1261 tcggcactgt gggaattggc atgatcgttg agccacagaa caacctcgga gtactcatcc
     1321 ataccagtat ctttaggaga atacagcaaa tatattccgc atatgactga tcaggaaatc
     1381 ttttccgatc tctcccgcat cggaaaacga ctatttatcg aacatctcgt cggcggaaac
     1441 tttggaaata tgagcgcacg ctacgctgac ggctatttta ttacacggac cggttcctac
     1501 ctcgatgcag atccggcaca ggtagttctc atgccgttaa acggcagagt tactccggga
     1561 gcatccagtg agtggcgggt acacacagca atctacaacg aagcagaaca gcatgcagca
     1621 atcgttcatg cccacccgcc ctatgcagtc gcactctccc tactggtgga aaatgaaatc
     1681 cttaccatcg acagtgaagg caaaatgctc gccccgtcca ttgcagtggt ggacggagca
     1741 ccgggaacac aggaactcgc tgacgcagtt gcagcgggat tatcttgcgc aaacgttgtc
     1801 attgcaagag gacacggaac atttgccgcg ggaaaggatc tggatcaggc atacctgtat
     1861 acctcgcttg ccgagcactg ctgtaaagtt ttacactaca caaaactcta ccggcagtaa
     1921 acaaccagaa aaaaatattt tttttagagg agaggatcga tggattcact gatccgcggg
     1981 actgagactg caccgactgg aacacatttt ccattaatca aaatgatcgg aagcggcggg
     2041 tggtcagcgc ggacaatgtc tttgatgcgg tcggggatct ctttgtcaag cgggaccagc
     2101 tcaacagaaa ccttatcccc atacttattc ttgagggcat cgcacaatgc cgggaaggca
     2161 aaagagagtt tttctttctg atag
//
LOCUS       DALUAJ010000140         7164 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_25187,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000140 DALUAJ010000000
VERSION     DALUAJ010000140.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25187"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            169..1731
                     /locus_tag="O0X37_02985"
     CDS             169..1731
                     /locus_tag="O0X37_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HJK61923.1"
                     /translation="MDEHTIHREIISLIFASSDPDIQSIKLSVCRKYSLDAMPKNSAI
                     LAAATPDEYEAVRRVLQVKPTRTLSGVAPIAVMTSPCACPHGKCLPCPGGPEHEFRSP
                     QSYTGEEPAALRARQNEYDPYRQVTARLGQFKLLGHHVDKAELIIMGGTMTAREPSYQ
                     EWFVSECLRGMNEFSGKTSVAHNLAELMDENEHAAVRCIATTFETRPDWCREEHINRM
                     LDLGVTKVELGFQHTDDDLLMQNKRGHTVANSVEANRLLRDAGIKVGFHVMPNLYGSS
                     IPRDQKMFDTLFTDPRFCPDFLKIYPTLVTPGAELEELWKSGEYETYDEDDLIDLLAY
                     AKERLPEYVRLQRIQRDIPAKLIVSGSIHGHIRQMAQKRLAEQGKQCHCIRCREIGRR
                     PSHAPVEEKSLIYDCCGGTEHFLSTVSGDALIGFVRLRFPGTVFRSELDGAALVRELH
                     VYGSIVPLGEHGEGEDRQHRSYGQKLLSRAEDTARNAGYDKVAVMSGVGVRPYYRKQG
                     YVRTGPYMIKNL"
     gene            1728..2873
                     /gene="priS"
                     /locus_tag="O0X37_02990"
     CDS             1728..2873
                     /gene="priS"
                     /locus_tag="O0X37_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase catalytic subunit PriS"
                     /protein_id="HJK61924.1"
                     /translation="MKPATYEFLRQRFSAYYNGEVQGAGAVYIPEALTEREWGFIFFS
                     SGSKTAGMRRHLSFSSPEEVHTYLKTMIPAHVYYSTAYYARPGAGQMSDKGWLGADLI
                     FDLDADHIVRGPYDVMLARVKEELFKLIDMLTSELGFFERDLRINFSGGRGYHIHIPT
                     LAVRSWDSTERRELVNYVSGTGLSAESMLAGPARNKGWQKRYRAALLAELDRIAALES
                     DAQAEYLTGFKGISDKFATGFLKNLDATRTTAATIPEKLLENKVIRAITNPENKPFQD
                     QILAMAAQADEPVTTDIKRLIRHPGSLHGGSGMRVTPLEVDRLDAFDPLLDAVVFGER
                     QVTVECSFPLTMPILGNSYSLSKGLNTVPEALAVFLCCRGIAEIGGV"
     gene            2875..3576
                     /locus_tag="O0X37_02995"
     CDS             2875..3576
                     /locus_tag="O0X37_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication complex GINS family protein"
                     /protein_id="HJK61925.1"
                     /translation="MPDRISISELHGYLIDERNSGALTEIPATLYEEVHAELNALTSE
                     ARSMGDPFGEGVQILLKERESLREYIRDLYAERTRKIFSLALAKATGEEINREELRCM
                     VPGERSLYEVVADSATACRKALLDGKQILDTTTAFHFSPGEAGIVADEPNSTDAASFA
                     DAHADEVFQNGNADAETAPESYRVIMVQEKVEEFQDYSGRCYALSPGDIVSLPQPMAE
                     ILCNRNIALNIRLRK"
     gene            3618..3896
                     /locus_tag="O0X37_03000"
     CDS             3618..3896
                     /locus_tag="O0X37_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L44e"
                     /protein_id="HJK61926.1"
                     /translation="MKKPVKFNTYCPYCRKHTEHEVEKVKKGKTTGLHWIDRQKARRS
                     SVGNRGKFGKVPGGDKPTKKINVRYRCKECGKAHLRAGYRAGKFELTE"
     gene            3903..4091
                     /locus_tag="O0X37_03005"
     CDS             3903..4091
                     /locus_tag="O0X37_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S27e"
                     /protein_id="HJK61927.1"
                     /translation="MVKSTRENRSKFLKVKCPDCEHEQLVFEKASSKVECIVCGRVLA
                     EPTGGKADIKADVIASYE"
     gene            4109..4882
                     /locus_tag="O0X37_03010"
     CDS             4109..4882
                     /locus_tag="O0X37_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit
                     alpha"
                     /protein_id="HJK61928.1"
                     /translation="MSERDWPIEGELVVCSVVEVKDFAAFVALDEYEGRQGLIPIAEI
                     ARGWIKYIRDYIREGQKVVCKVLHVDESRGHIDLSLKDVNEHQRREKIQDWKNEQKAH
                     KWIEFASKDSNVPVKDIEEAMYKEYSSLYAAFEDIAIYGDTTLEKFSLPEVVRNALAK
                     VASENVKVPRVTVSAILELTSNKPDGVNVIRRALRSAEPKVDGAEIELLYLGAPKYRV
                     KVVAPDYKTAEKALEKAANAAIGVMERADGTGKLVRKQK"
     gene            4894..5055
                     /locus_tag="O0X37_03015"
     CDS             4894..5055
                     /locus_tag="O0X37_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-protein complex protein Nop10"
                     /protein_id="HJK61929.1"
                     /translation="MTGHIRQCPIDHTYTLLKVCPKCGAVTESVHPARYSPQDKYGKY
                     RRMIREWNK"
     gene            5043..5801
                     /locus_tag="O0X37_03020"
     CDS             5043..5801
                     /locus_tag="O0X37_03020"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome assembly chaperone family protein"
                     /protein_id="HJK61930.1"
                     /translation="MEQVNVRWYVEDVSAHSASVVIAGLPGVGHVGKLVVDHLVRALS
                     AVKVAEITSTLFPPQMYLDCDAVLRLPRNEIFFVPGTDEHDPVLFLAGDCQSTSAEGH
                     YTLAEGYLRVFDLLGVRRIYTLGGYGVGRMVEHPRVLSALSSASLKEEVLAAGAVINP
                     EEPVGGIIGAAGLLITLGRLHGMEGIALLGETSGYLVDPVSSTAVLDVLERLTHIHAD
                     RTELTDLAEQMMTDIAAIANAAMPKPPDDLSYIG"
     gene            5807..6925
                     /locus_tag="O0X37_03025"
     CDS             5807..6925
                     /locus_tag="O0X37_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014868339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease Q family protein"
                     /protein_id="HJK61931.1"
                     /translation="MQKNTDLHLHSCFSMATSPDMVPEHILAGCRIKGIDVIGSGDAL
                     HPEWRKMWEPYVDNEFGITVIPQTEVEDSSRVHHVILAETFDQFAELQDMFEPVCGHL
                     TTAGRPHLQLGGEEIATAVHDVGGMIGPAHAFTPWTSLFAAFTSPSECYGEETFEFCE
                     LGLSADSTYGAGIAEFADVPFLTNSDAHSPTPIKLGREFTRMDLSTDSPKSVLAGISS
                     GNIILNAGFFPEEGKYNRTACTRCYQQFSLAEAEHFHWKCPHDNGRIKLGVRERAEKL
                     STLAEPTQRPPYLKMIPLGEMIARVLGTASPNTKKCKELYNRFVETFDNEIAVLLEIP
                     KQDLCSVSCEVADAVIKMREGQVTLFPGGGGKYGSFAF"
     gene            complement(6966..>7164)
                     /locus_tag="O0X37_03030"
     CDS             complement(6966..>7164)
                     /locus_tag="O0X37_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HJK61932.1"
                     /translation="PVQLPMMCIRDHGVSRCRLVMDPFMGIGSTAVAAVRLGIPFIGF
                     EIDEGYINIAEERIAAELLPQ"
BASE COUNT         1967 a         1782 c         1851 g         1564 t
ORIGIN      
        1 tatcccctat ccatatccat aaaaataaga atcttcaccg tgttgccctc cttacgcttg
       61 gaaacgagat cgttaccgag atgaacgtcc acaaaaagcc ccagacttca tttccccaag
      121 agtccggtgc gggcgtcgtt gcagaacact aactcttttc cctcaaatat ggacgagcat
      181 acaattcacc gggagataat ctccctcatc tttgcctcgt cagacccaga cattcaaagc
      241 atcaagttat ccgtctgtcg gaaatactcc cttgatgcca tgccaaaaaa ctcggcaatt
      301 cttgcagcgg caaccccgga tgagtacgag gcagtccgtc gggtacttca ggtaaaaccg
      361 acgcgaacac tatccggagt tgcaccaatt gctgttatga cctccccatg tgcatgcccg
      421 catggaaaat gtctcccctg tccaggagga ccggaacatg agttccgatc accgcagagt
      481 tacactggag aggaaccagc cgctcttcgt gccaggcaaa acgagtatga cccctaccgc
      541 caggtaacag cgcggcttgg acagttcaaa ctcctcggac atcatgtaga taaagccgaa
      601 ctgattatca tggggggaac catgaccgcg cgtgaaccct cataccagga atggttcgtc
      661 tccgagtgtc ttcgcgggat gaacgagttc tcaggaaaaa catctgttgc acataatctc
      721 gcagaactca tggatgagaa cgagcacgca gcagtacggt gtattgcaac cacctttgaa
      781 acaagaccgg actggtgccg tgaagagcac ataaacagga tgcttgatct cggagtcacc
      841 aaagtcgaac ttggattcca gcacaccgat gatgacctgc tgatgcaaaa caagcgcggt
      901 cacaccgttg ccaacagtgt cgaagcgaac cgtctattgc gtgatgcagg gatcaaagtg
      961 gggttccatg tcatgccaaa tctctatggc agcagcattc cgcgcgatca gaaaatgttt
     1021 gatacgctct tcacagatcc aaggttctgc ccagattttc tcaagatata tccaactctc
     1081 gtgacacccg gagcagagct tgaagaactc tggaaatcgg gagagtacga aacgtatgat
     1141 gaagatgacc tcatcgatct tctcgcctat gccaaagagc gacttcccga atatgtccga
     1201 cttcagcgga ttcagcgcga catcccggca aaactcattg tatccggatc catacacggc
     1261 cacattcgcc agatggcaca gaaacgcctt gcagaacagg gaaagcagtg ccattgtatc
     1321 aggtgccgtg agattggacg aagacccagc catgcacctg ttgaagaaaa atccctgatc
     1381 tatgactgct gtggcggaac agaacacttt ctctccacag tctcgggaga tgccctcatc
     1441 ggatttgtac ggctgcggtt cccggggacg gtcttccgat cagagcttga tggggcagca
     1501 ctcgtccgtg aactacatgt atacggcagt atcgttcccc tcggcgaaca cggagaggga
     1561 gaggacagac aacatcgatc ctacggccaa aaactgttgt cccgcgcaga agatactgca
     1621 aggaatgcag ggtatgacaa agtcgcggtt atgagcggtg taggggtacg accctactat
     1681 cgcaaacagg gatatgtgcg gacaggccca tatatgatca agaacttatg aagccagcca
     1741 cctatgagtt cctcaggcag cggttctctg cctactataa tggagaggtt cagggagcag
     1801 gagctgtgta tatccccgag gcacttactg aacgggaatg gggcttcatc ttcttttcaa
     1861 gcggatcaaa aacagccggg atgcgcaggc atctttcctt tagttcacca gaggaggtac
     1921 acacctatct caaaaccatg atccccgcgc atgtttacta ctcaaccgcg tactacgcgc
     1981 ggccaggagc ggggcagatg agtgataaag gatggctggg agcagatctt atttttgacc
     2041 ttgatgcgga ccacatagtc agaggaccgt atgatgtcat gcttgcgcgg gtcaaagagg
     2101 agctattcaa gctcatcgac atgctgacat cggaactcgg gtttttcgag cgcgacctca
     2161 ggatcaattt ctccggtggc cgcggttatc atatccacat accaaccctt gcagtccgca
     2221 gttgggacag tactgagcgg cgagaactgg tcaactacgt ctcaggaaca ggtctttccg
     2281 cagaaagcat gcttgcaggt cctgcgagaa acaaaggctg gcaaaagcgt taccgtgcag
     2341 cacttctcgc agagcttgac aggatagccg cactggagtc tgatgcacag gcagaatacc
     2401 ttaccggatt caaagggatc tccgacaagt ttgctacagg gtttctgaaa aatcttgatg
     2461 caacccgtac aaccgccgca acaatcccgg aaaagcttct cgaaaacaaa gtcatccggg
     2521 cgattacaaa tccagaaaat aaaccatttc aggatcagat cctagcaatg gcagctcagg
     2581 cagatgaacc ggttacgact gacattaagc gtctcatccg ccaccctggt tctctgcacg
     2641 ggggttccgg tatgcgggta actccccttg aggttgaccg tcttgatgca tttgatccgc
     2701 ttttggacgc cgtggtcttt ggcgagcgac aggtaacagt tgagtgttcc ttcccgctga
     2761 caatgccgat acttggaaac agctacagtc tttccaaagg attaaacacc gtccccgagg
     2821 cacttgcagt attcctctgc tgtcgcggaa ttgcagagat cggaggagtg taatatgcct
     2881 gaccgcatca gcatctcaga acttcacgga tatctgatcg acgagcgcaa cagcggagcg
     2941 ctcaccgaga tacctgcaac cctgtatgaa gaggtgcatg cggaactcaa tgcattaacc
     3001 agtgaagcgc gcagcatggg agatccattc ggagaaggag tacagattct cttaaaagaa
     3061 cgtgagagtc tgcgcgagta catccgggat ctctatgccg agcggacccg caaaatcttt
     3121 tcactggccc tcgccaaagc aaccggggaa gagattaacc gcgaagagct ccgctgcatg
     3181 gtacctggag aacgttcact ctatgaggtt gtcgcagact cagctacggc atgcagaaag
     3241 gctcttcttg acggtaaaca aatattagat accacaaccg cattccactt cagccctgga
     3301 gaggcaggga tagtggcaga tgagccgaat tcgactgatg cagcctcttt tgcggacgcg
     3361 catgcagatg aagtgttcca gaacggaaac gccgatgcag aaactgctcc cgaatcgtac
     3421 cgcgtgatta tggtacaaga aaaagtagag gaatttcagg attatagcgg acgatgttat
     3481 gctctttcgc caggagatat cgtttcgctg ccacaaccca tggcagaaat tctctgcaac
     3541 cgcaacatag cattaaatat caggcttcgc aaataagtat ggtattcggt atccttgatt
     3601 tagaggggac aatattcatg aaaaaaccag taaaattcaa cacctactgc ccgtactgcc
     3661 ggaagcacac cgagcatgag gtggaaaagg taaagaaggg taagacaacc ggcctccact
     3721 ggattgaccg tcagaaggca cgccgcagct ctgttggaaa ccgcggtaag ttcggtaagg
     3781 ttccgggagg agacaagcca accaagaaga tcaacgtacg ttaccggtgc aaggagtgcg
     3841 gaaaggcaca cctgagagca ggataccgcg ctggcaaatt tgaacttacg gagtgagcaa
     3901 gaatggtaaa atcaacccgc gaaaaccgga gcaagtttct gaaggttaaa tgcccagact
     3961 gcgagcacga gcagctcgtc tttgagaagg catcctctaa agttgagtgc atcgtctgcg
     4021 gacgtgtcct tgcagagccg actggcggta aggcagacat caaagcagat gtcatcgcat
     4081 cttacgaata aattcggaac catcatcaat gagtgaaaga gactggccaa ttgaaggaga
     4141 acttgtcgtc tgcagtgttg tagaggtcaa ggactttgcg gcattcgttg ccctggatga
     4201 gtatgaagga cgtcaggggt taatcccgat tgcggagatt gcccgtggat ggatcaaata
     4261 catccgggac tatattcgtg aaggacaaaa agtcgtatgc aaagtccttc atgtggacga
     4321 atcccgcggc catattgatc tttccctcaa agatgtaaat gaacaccagc gccgtgaaaa
     4381 gattcaggac tggaagaacg aacaaaaagc ccacaaatgg atcgagtttg cctcaaagga
     4441 cagtaatgtc ccagtgaagg atattgaaga ggcaatgtac aaggagtact cctctctcta
     4501 tgctgcattt gaggacatcg ccatctatgg tgacacaaca ctggaaaagt tctccctgcc
     4561 tgaggtcgta aggaacgcac tggcaaaagt tgcatcagaa aatgtgaaag ttccccgggt
     4621 tacggtttcc gcaatcctgg aactgacctc caataaaccg gacggagtaa acgtaattcg
     4681 acgcgcactc cgttccgctg aacccaaagt tgacggtgcc gagatcgagt tgttgtatct
     4741 gggcgcaccc aaataccggg taaaggtcgt tgctccggac tacaaaacag cagagaaggc
     4801 tctggaaaaa gctgcaaatg ctgcgatcgg ggtgatggag cgcgccgatg gtaccgggaa
     4861 actggtacga aaacagaaat aactaggtgt attatgactg gtcacattcg gcaatgcccg
     4921 attgatcaca cctatactct tttgaaagtt tgtccgaaat gtggtgctgt aactgagtca
     4981 gtgcaccctg cacgatattc cccacaggat aaatatggta aataccggag gatgatacgc
     5041 gaatggaaca agtaaatgtg cgctggtatg tagaagatgt atctgcccac tctgcaagcg
     5101 tggtaatagc tggtcttccg ggtgttggcc atgtgggaaa actggtggtg gatcatctgg
     5161 tccgtgcact ttccgcagtg aaggttgcgg agataacttc tacactgttt ccgccgcaga
     5221 tgtatcttga ttgcgatgct gttcttcgcc ttccacgcaa cgagatattt tttgtgccag
     5281 gaaccgatga acacgatccg gtgcttttcc tcgcgggtga ctgtcagagt accagtgccg
     5341 aagggcatta cactcttgcg gaaggatatc tgagagtgtt tgatctgctg ggcgtccgcc
     5401 gcatctatac gctgggcggt tacggtgttg gaagaatggt cgaacacccg cgggtacttt
     5461 cagcactgag ttctgcatcc ctcaaagagg aagtacttgc agccggtgca gtgataaatc
     5521 ccgaggaacc ggtaggaggc attatcggtg ctgcagggtt acttattacg ctcgggcgct
     5581 tgcacgggat ggaaggaatt gcgcttcttg gagagacctc cggttacttg gtagacccag
     5641 tgagctcaac agcagtactg gatgtactgg agcgactaac acacattcat gcagaccgga
     5701 cggaactgac ggatcttgct gagcagatga tgactgatat tgcagcaatt gcaaatgctg
     5761 caatgccaaa acccccggac gatctcagct acatcgggtg agacgaatgc agaaaaatac
     5821 agacctgcat ctgcactcct gtttctccat ggcaacgtct ccggacatgg tgccggaaca
     5881 cattctggcg ggatgcagaa tcaaaggcat tgatgttatc ggaagcggcg atgcactgca
     5941 tcctgaatgg cgcaaaatgt gggagccgta tgtggacaat gaatttggga ttacagtgat
     6001 accacaaacc gaagtggagg actcctcacg cgtacatcac gtaattctcg cagaaacatt
     6061 tgatcagttt gcggaactgc aggacatgtt cgaaccggta tgcgggcatc ttactaccgc
     6121 agggagacca catcttcagc tgggaggaga agagatcgca acagcagtcc atgacgtggg
     6181 aggaatgatt ggtcctgcac atgcatttac tccgtggacg tcattgtttg cagcattcac
     6241 atccccttcc gaatgctatg gggaagagac gtttgagttc tgtgagcttg gactctcagc
     6301 agattccacg tacggtgcag gtattgcaga atttgccgat gttccattcc ttacaaactc
     6361 agatgctcac agcccgacac caataaaact cgggcgcgag tttacacgaa tggatctttc
     6421 cacagactcg cctaaatcag ttctcgcagg aatttcctca ggaaacatca tcctgaacgc
     6481 cggatttttt ccggaagagg gaaaatacaa ccgtacggca tgtactcgct gttaccaaca
     6541 gttttcactt gcggaggcag agcacttcca ctggaaatgc ccgcatgaca acggaagaat
     6601 caagcttggt gttcgtgaac gcgcagaaaa actctccaca cttgcagaac caacacaacg
     6661 tccgccatat ctgaaaatga ttcctttggg agagatgatt gcacgcgtac ttggaacggc
     6721 ctcaccaaac acgaaaaaat gcaaagaact gtacaacaga ttcgtcgaaa cattcgacaa
     6781 tgagattgca gttctgctcg agattcccaa acaggatctc tgttccgtat catgtgaagt
     6841 ggcagatgca gtgataaaaa tgcgggaggg acaggtcaca ctcttcccag gcggcggcgg
     6901 gaaatacgga tcatttgcat tctgaatatt tacggctgac aaaatgaaaa caatgggcag
     6961 ggctctcact ggggaagcag ctcagctgcg atccgttcct cggcaatgtt gatgtatcct
     7021 tcatcgatct caaacccgat aaacgggatc ccaagacgaa ctgcggcaac agcagtactg
     7081 ccaataccca taaacggatc catgacaagg cggcaacggc tgacaccgtg atcacggata
     7141 cacatcatcg gcagctgaac cgga
//
LOCUS       DALUAJ010000141         1535 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_25574,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000141 DALUAJ010000000
VERSION     DALUAJ010000141.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25574"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="O0X37_03035"
     CDS             <1..778
                     /locus_tag="O0X37_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HJK61933.1"
                     /translation="SIATEVLEGRGTEHGGVWLDVTHLPREQIETRLPLMLEQFLQFG
                     VDIRTQPMEVAPTAHHFMGGLRITPEAHTTLPHLFAAGEVTGGVHGGNRLGGNALADT
                     QVFGRRAGLAAASVEHTAKRASDADQIAAAEEKISRLYEGSAASAEVRRSMKQIMWNN
                     VGIYRNELDLKVAERDIAKLRATPLGISSPREINDAIITENMLLVASLVIDGALLRRE
                     SRGAHTRTDVSVPWTNETSPFGHTFFSKARSGIEILEGRL"
     gene            775..>1535
                     /locus_tag="O0X37_03040"
     CDS             775..>1535
                     /locus_tag="O0X37_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HJK61934.1"
                     /translation="MKVQISRFDPDKDAAPHLETYDVHVENGARVLNVLDAVRDGIDP
                     TLGYRHCCRAGQCGSCAVRVNGEPVLACMEEAHDGDLIEPLSLPRIRDLITDIAPVMA
                     QMAWLNPGVEGISCSCGVTHDTIESIKPLRECIECYSCVSSCPAFAASEYAGPTIMRQ
                     EQRLNLDPRDGGDRVEEAIAKGLFACTTCHKCVEVCPKGIETPRKAVEKLRAEAAKRG
                     LSLPAHKALARLVEETGRSVERKETPFLERVPDVI"
BASE COUNT          348 a          386 c          448 g          353 t
ORIGIN      
        1 atcgatcgcc actgaggttc ttgagggtcg cgggacggaa cacggtggtg tgtggctgga
       61 tgtaacccat ctgccaagag aacagattga aacccgtctg cctttgatgc tggaacagtt
      121 tctccagttc ggtgtcgaca tccgtactca gccaatggag gttgcaccaa ctgcccatca
      181 cttcatggga ggtctgcgga taactcctga ggcccatacc acgctgccgc atctgtttgc
      241 cgcaggagaa gttaccggcg gtgtgcacgg aggcaaccgt cttggcggga atgcacttgc
      301 ggatacccag gtgttcggtc gccgtgcagg cttggccgct gcttcggtag aacatactgc
      361 aaaacgtgca tcagatgccg atcagattgc ggcagccgag gaaaaaattt cccggctcta
      421 tgaaggatct gctgcatctg cagaggtccg cagatcgatg aaacagatta tgtggaacaa
      481 tgttggcatt tatcgaaacg aacttgattt aaaagttgcc gagcgtgaca ttgcaaagct
      541 ccgggcaacg cctttgggca tatcttcacc acgcgagatc aacgatgcaa tcatcacgga
      601 aaacatgctg cttgttgcat cgcttgtgat cgatggtgca cttctccgcc gcgagtcacg
      661 cggtgcccat acgcggaccg atgtcagcgt cccgtggaca aatgagacct caccgtttgg
      721 tcacacattc ttttcgaagg caaggtctgg tattgagatt ctggaggggc ggctgtgaaa
      781 gtacagatct cgcggtttga tcccgacaag gatgctgcac cgcacttgga gacgtatgac
      841 gtacatgttg aaaacggtgc acgtgtacta aacgttcttg atgccgtacg cgacggaatt
      901 gatccaacgc ttggataccg gcactgttgt cgtgcaggtc agtgcggctc atgtgcagtc
      961 cgtgttaacg gagagccggt gcttgcatgc atggaggaag cgcatgacgg agatctgata
     1021 gagccgcttt ccctcccccg catcagggat cttatcaccg atattgcacc ggtgatggca
     1081 cagatggcct ggctgaaccc aggggtagag gggatttcct gcagctgcgg ggtaacacac
     1141 gatactatcg aatctatcaa accgcttcgt gagtgtattg agtgttacag ctgtgtatcc
     1201 tcttgtcccg catttgcggc atccgagtat gccggaccta ctatcatgcg gcaggaacag
     1261 aggttgaacc ttgatccgcg ggatggtggt gaccgtgttg aggaagcaat tgccaaaggg
     1321 ctctttgcct gtacgacctg ccacaaatgt gtggaggtct gcccaaaggg aattgagact
     1381 ccgcgaaagg ctgtggagaa acttcgagcg gaagctgcaa agcgtggcct gtctcttcct
     1441 gcccacaaag cacttgccag gcttgttgaa gagactggcc gctcggtcga acggaaggaa
     1501 actccgttcc ttgaacgtgt gccggatgtt atcga
//
LOCUS       DALUAJ010000142         2058 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_25588,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000142 DALUAJ010000000
VERSION     DALUAJ010000142.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25588"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..2027)
                     /locus_tag="O0X37_03045"
     CDS             complement(<1..2027)
                     /locus_tag="O0X37_03045"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HJK61935.1"
                     /translation="MVDELYHPGYIAKRMEVGAVIGAAPANNVRREVPAAGDVVILLG
                     GRTGRDGCGGATGSSKSHTAASLESCGAEVQKGNAPEERKIQRLFRNRDAAVLIKRCN
                     DFGAGGVSVAIGELADGVLINLNAVPKKYDGLDGTELAISESQERMAVVVAAADAQKF
                     ISLAHAENLEATIVAEVTAEPRLVMVWNGDKIVDISREFLASNGVQKHTDVEMADPQP
                     FSREISGDFAENYRNLAEDLNVCSKRGLSERFDSTIGAGAVLMPFGGKYQQTPVQAMV
                     TKISVEFGDTDDCSLMAWGCNPQILEKSPYHGAYLAVVESVSKLVATGAEFSDVYLSF
                     QEYFEKLGSDAQRWGKPAAALLGALKAQMGLSIGAIGGKDSMSGSFEQLNVPPTLISF
                     AVTTEKIPHIISNEFKGSDHRVILLSPIYEDDLPTTDSLLSVFSQVTGLMREGRVLSC
                     WTPTYGGVAEGVLKMGLGNKIGFADDASIGMGDIFGYRYGSFILELADDTDVGVLLGR
                     TTDAYELRFGSETVSLDEIQDAYEAKLEPVFACNISQGDGVIEKISYTERNTAKPRVR
                     VAHPRVLIPVFPGTNCEYDSAKAVSRAGAVPEIVIINNMTAQDTAESVDRVADAIRRS
                     HAIFIPGGFSGGDEPDGSGKFITAFFRNAKIKDAVHDLLKNRDGLMCGICNV"
BASE COUNT          520 a          587 c          493 g          458 t
ORIGIN      
        1 acgttgcaga taccgcacat caatccatcg cgattcttca gaagatcatg caccgcgtcc
       61 ttgatctttg cattgcggaa gaacgccgtg atgaatttac ccgagccgtc gggttcatca
      121 ccacctgaga aacctccggg aatgaagatt gcatgcgaac ggcgaatagc atccgccacg
      181 cgatccacgg actcagcggt gtcctgagca gtcatgttgt tgatgatgac aatttcagga
      241 accgcaccgg cacgagagac agctttagca ctgtcatact cacagtttgt acccgggaag
      301 accggaatga gtacacgggg atgggcaaca cggacacggg gttttgcagt gtttcgttcg
      361 gtataggaga tcttctcaat tacaccatct ccctgggaga tgttgcaggc aaataccggt
      421 tcaagttttg cctcataggc atcctgaatt tcatcgagcg aaactgtttc agaaccgaaa
      481 cggagctcat aggcatcggt agttctgcca agcagaactc ccacgtccgt atcatcggca
      541 agctcaagga tgaatgaccc ataccggtag ccgaaaatat cgcccatccc aatggatgcg
      601 tcatcggcaa aacctatctt attcccaaga cccatcttca gaacaccttc cgcaaccccg
      661 ccatacgttg gggtccagca ggaaagcacc cggccctcgc gcatcagacc tgtcacctgt
      721 gaaaagacgg aaagcaggga gtctgttgtc ggaagatcat cctcataaat tggagagagc
      781 aggatcacac ggtgatctga ccccttaaac tcatttgaga tgatatgcgg gatcttctcc
      841 gtggtcacgg caaacgagat cagtgttgga ggtacgttga gttgctcaaa ggatccgctc
      901 attgagtctt tgccaccaat agcgccgata ctaagtccca tctgtgcttt gagtgcacca
      961 aggagagcag cagcgggttt tccccaacgc tgtgcatcag aaccgagctt ttcgaagtac
     1021 tcctggaagg aaagatacac atccgaaaac tcggcaccgg tagcaaccag cttggatacg
     1081 ctttcaacta ctgcaagata tgccccgtga taaggactct tctccagaat ctgcgggttg
     1141 catccccatg ccatcagaga acagtcatcg gtatcaccaa actcaacgga aatcttggtg
     1201 accattgcct gcaccggcgt ctgctggtat tttccgccga acggcatcag cacggctccg
     1261 gcaccaatcg ttgaatcaaa ccgctcggag agtccgcgct tggaacagac attcagatcc
     1321 tctgcaagat tccggtagtt ctctgcgaag tccccggaga tctcgcggga aaacggctgc
     1381 ggatctgcca tctccacatc agtgtgcttt tgaacaccgt ttgatgcaag gaactcacgt
     1441 gagatgtcaa caatcttgtc cccgttccag accatcacca gacgcggttc ggcggtgacc
     1501 tctgccacaa tcgtagcctc aagattttcc gcatgtgcaa gcgagataaa tttctgagca
     1561 tctgcagccg caacaacaac ggccatgcgc tcctgcgact cactaattgc aagctccgtt
     1621 ccgtccagac catcatactt tttggggacc gcattaagat tgatcaagac accatcggca
     1681 agttctccaa tagcgacaga aacgccaccg gcaccaaaat cattacagcg tttgatcaaa
     1741 actgcggcat cgcgattacg gaacagccgc tggatcttcc gctcttcagg tgcatttcct
     1801 ttttgtacct cagcaccgca ggactcaaga gatgccgcgg tgtgagactt ggatgaacct
     1861 gttgctccgc cgcacccgtc acgtccggtt ctacctccga ggagaatcac cacatctcct
     1921 gcggcaggaa cttcacggcg cacattgttg gccggtgcag caccaataac ggcaccaacc
     1981 tccattcgtt ttgcgatgta accaggatga taaagttcat ccaccatacc ggtggctagc
     2041 cctatcgggt ttccatag
//
LOCUS       DALUAJ010000143         8242 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_25740,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000143 DALUAJ010000000
VERSION     DALUAJ010000143.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 8242)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8242)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8242
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25740"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="O0X37_03050"
     CDS             <1..463
                     /locus_tag="O0X37_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HJK61936.1"
                     /translation="STEEFQRVLDVNVTGTFLCTREVAKLMIAQNKGGAIVNFSSTFA
                     VVGSPGYLAYHASKGAIASFTRAVAVALLPYGIRVNAVAPGTTETPGLYDGARDTGNL
                     ETGLAGFLALQPLKRFGRPEEIAEVVLMLASDEASFVYGAVWMADGAYTII"
     gene            complement(477..791)
                     /gene="hisE"
                     /locus_tag="O0X37_03055"
     CDS             complement(477..791)
                     /gene="hisE"
                     /locus_tag="O0X37_03055"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HJK61937.1"
                     /translation="MTDAHVFDELWQVICQRATEDPGKKSYVRHLLFHEKGIDKPLEK
                     VGEESCEFVLAVKNGREEAIVGEAADLLFHLMVALKAADVDFAKVEEELEIRRAGMHL
                     HD"
     gene            complement(788..1237)
                     /locus_tag="O0X37_03060"
     CDS             complement(788..1237)
                     /locus_tag="O0X37_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusA-like transcription termination
                     signal-binding factor"
                     /protein_id="HJK61938.1"
                     /translation="MERTIGFKERRYIEELRILTKATAIDCLIDDRFDRIIYVIKQGD
                     MGLAIGKSGDNIKKMSRVLGKRIEMVEFSEEPDHFIANMFKPAEILDVQFGGDDGPVT
                     VLVPEKSDVGLAIGKGGSTIEKARQLVRRFYCKDIGNIAVPGDAETT"
     gene            complement(1335..2768)
                     /gene="proS"
                     /locus_tag="O0X37_03065"
     CDS             complement(1335..2768)
                     /gene="proS"
                     /locus_tag="O0X37_03065"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HJK61939.1"
                     /translation="MADDTEGLPPRSDFSAWYNEVIRRAEIMDVRYPVKGLYVWYPFG
                     FALRNHTYTLLRSLLNRDHEETLFPLLIPETEFMKEAEHIKGFEDEVYWVTHGGLSPL
                     DVKLALRPTSETAIYPMYALWIRSHADLPLKLYQIVNTFRYETKHTRPLIRLREITSF
                     MESHTVHATWDEANEQVEYELALSQEFYRDLGVPIIISKRPDWDKFPGADFTMAIDAV
                     MPDGRTLQIGTVHHLGDHFSKTFGITYEDVNGEQQFGSQTCYGISERCIAAIIGVHGD
                     DKGLVLPATVAPTQVVIIPVIVGKRGDEILAAVKTLESELKSAGLRVKTDSRDMRPGA
                     KYYHWELHGVPLRVELGPRDLDNNQLVCVNRLGVKTVIPRENAAESVKKLLDEAHDQI
                     LERAENHLESHLMTAVTPEECNEKLDGNVVVVHWCGCRDCADKLEELTNSSLLGTEVR
                     SKYVVDDEGPCIICGKKGKAALVGRSY"
     gene            complement(2947..4104)
                     /locus_tag="O0X37_03070"
     CDS             complement(2947..4104)
                     /locus_tag="O0X37_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HJK61940.1"
                     /translation="MRLAHLFSALEMSDSSWDTALIFGRINMYYLTGTIQDGVLVLRR
                     NGDVLFFVRRSFERAQAESPLGTVYPMKSYRDMRDYLPSDLGRIFVETDQVPVAVLDR
                     LKKYFRFETVLALDPVMLSLRAVKSQYELEFLRCAGREHDHLLMKIVPEILYEGMSEA
                     QLFGELYHAMMDLHYQGVVRFSGFGTEMQIGQMGFGTTTLEATCFDGPGGMLGASAIV
                     PSVGSRDRLLAKGDLVFVDIGYGYNGYHTDKTQVYSFQGKLPDHALKLHEECIAVQRK
                     AASLLAAGNIPSEIYAEILNSISPELSENFMGYKSRRVSFLGHGIGLVVNEWPVIALG
                     FDSPLTENMVIALEPKSGVPGVGTVGVEDTYVVGRSGGECITGGGRSVLEI"
     gene            complement(4250..4828)
                     /locus_tag="O0X37_03075"
     CDS             complement(4250..4828)
                     /locus_tag="O0X37_03075"
                     /inference="COORDINATES: protein motif:HMM:NF014052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HJK61941.1"
                     /translation="MLSDRCLYIIALAVVVVVFFGSCIYGYAESIEMPAMADEIVVTF
                     QSSTTELDSSMPALLSWQIFFNNLKVCLILFIGGVTFGVVTLFVLVSNGYVIGSISSV
                     MLRGYDVSVFAATLVPHGIFEIAAILMAAALGLQTGRSLYLDAQGRDNAGKTCLWYGT
                     RFLLVVVPLLVIAALVESFVTPEIAMLVLSGV"
     gene            complement(4828..5685)
                     /locus_tag="O0X37_03080"
     CDS             complement(4828..5685)
                     /locus_tag="O0X37_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF63 family protein"
                     /protein_id="HJK61942.1"
                     /translation="MFNEIGAFIQKYYIDAVLEDQPYNIYLTITCAILLLVTLYLLYR
                     WARANNIRVDTQLILASMPFVIFGGVLRVVEDTGFIPEPWWVLFVTPPIFFLVLLYTI
                     FVLVLSRTLENHGIVASYTKPYFWVGVISNLACLVFLCWWGITQTELALWVAGCILVM
                     ATVVASLFWALLRYGFSWEYVTHPLYKILIFGQLLDASATSFALDLHPMHYIEQHVLG
                     DALIQLTGTAFVMFPLKMVVLIPAIWVLEKFRNEEGMDEVWHLVLLAMITVGFAPGVR
                     DILRMVLFI"
     gene            5701..6783
                     /locus_tag="O0X37_03085"
     CDS             5701..6783
                     /locus_tag="O0X37_03085"
                     /EC_number="1.1.1.261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-1-phosphate
                     dehydrogenase"
                     /protein_id="HJK61943.1"
                     /translation="MNVDNNRILKDKTFDKSRWIQMPRNVVVGHDALLQIPGIIDDLG
                     VAGPIMLLSGPTTMQTVGARVAELLVGHSITTCLVGRITYDEIQRIEDEAKKTGVVLI
                     IAVGGGRVIDTAKVVSYNLDIQFVSVPTAASHDGIASSRASVVTDSGNVSVAAQPPLA
                     IVADTGIIATAPHRLLAAGCADIIANYTAILDWELSHRLTGETISEYALTLSKITAEI
                     LVNNTDLVSHNDETAVWIVVKALFSSGIAMSIAGSSRPASGGEHKFAHMLERLAPGSA
                     LHGEACGLGTIIGMYLHGGDWKGIRTSLKRIGAPTTPQDLGISNEICVEAVLRACEIR
                     PERFTIFDTGITREAAVAAVEALYEV"
     gene            6790..7251
                     /locus_tag="O0X37_03090"
     CDS             6790..7251
                     /locus_tag="O0X37_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0179 family protein"
                     /protein_id="HJK61944.1"
                     /translation="MDDTEKIVTIVGSVLAHEGAEFVYAGKITECDNCKVGKVCHNAK
                     LREGRRYRVIAVRPTKHECFVHAGGAVAVEVAEAPITAVIPTSQATRRTRITYTPVCD
                     DRFCKGYAFCHPDGMTDKGRYVVLEVLGSYNEMCPMGKKNLKLVELRHVPT"
     gene            complement(7217..8089)
                     /locus_tag="O0X37_03095"
     CDS             complement(7217..8089)
                     /locus_tag="O0X37_03095"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HJK61945.1"
                     /translation="MTFISEKERIFGGLFGVAAGDAVGLTFEGMMPGPRYNLSVTGGG
                     QFSLPAGAVTDDTMMTLSLLETYLSYGTFDREAFLLRMTRVVLQNPVTFGRTTKTLCV
                     LLEQGCVSDEAVKVVDLLFGSRTNGSVMRTLPVGMICTPETVASEARRVSAFTHYDPI
                     AGDCCAAVSAAAAVLLAGGDKDEALAAAWDASGRSDLFSGALIPSVDAVAATRCAFSC
                     FRDSESVRDCIECAVTLGGDTDTIASISGGLAGLYFGINDLPTEWVCRINIRKKITEC
                     ANALMLARGAAQRV"
BASE COUNT         2272 a         2192 c         1875 g         1903 t
ORIGIN      
        1 gagcacggaa gagttccagc gtgttctgga cgtaaacgtc acaggcacat ttctctgtac
       61 ccgggaggta gcaaaactca tgattgcaca gaacaaaggc ggtgcgatcg tcaacttttc
      121 ctcgaccttc gccgtagtcg gctccccagg ctaccttgct taccatgctt ctaaaggagc
      181 aattgcatca ttcacccgtg cagtagctgt cgcactcctg ccgtatggaa tccgtgttaa
      241 tgcagttgca ccgggaacta ccgagactcc cggtctctat gacggtgcgc gagataccgg
      301 caatttggag acaggtcttg ccgggttcct tgcacttcag ccactcaaaa gattcggcag
      361 accggaagag atcgcagagg tggtacttat gctcgcaagt gatgaggcat cctttgtcta
      421 cggagcagta tggatggctg acggtgccta cactataatc taaacctcaa aaaaagttag
      481 tcgtggaggt gcattccggc acgccggatt tcaagctcct cctccacctt tgcaaaatcc
      541 acgtccgcgg ccttgagggc aaccatcaaa tggaacagca gatccgcagc ctcaccaaca
      601 atcgcctcct cacgtccgtt tttcaccgca agcacaaact cacaggactc ctcgccaacc
      661 ttttcaagcg gtttgtcaat ccctttctca tgaaacaaaa gatgacggac atacgatttt
      721 ttgcccggat cctctgttgc acgctggcag ataacctgcc acaactcatc aaacacgtgt
      781 gcatctgtca tgttgtctca gcatctcccg gaactgcaat gtttccgata tctttacagt
      841 aaaatctgcg aaccagctga cgcgcctttt cgatggttga tccaccttta ccgatggcaa
      901 gaccaacatc acttttctca ggaacaagga ccgttaccgg accgtcatct ccgccgaact
      961 gtacatccag gatctcagca ggcttgaaca tgttggcaat aaagtgatcc ggctcctcgg
     1021 aaaactcgac catctcgatc cgttttccga gaacccgtga catctttttg atattatcac
     1081 cgctctttcc gattgcaagt cccatgtcac cctgcttaat cacatagatg atccgatcaa
     1141 atcgatcatc aatcaggcag tcaatcgcag tagcctttgt taaaatccgg agctcctcga
     1201 tataccggcg ttctttaaat ccgatagtcc gctccattgc atatttgtca ataatatcca
     1261 tacgtatcag aaatttacct attgcaacaa ttgatgcggg gacatgcccc gcagtattcg
     1321 agaatgattt tgggttagta ggatcttcct acaagagccg ctttaccctt cttaccgcag
     1381 atgatacatg ggccctcatc atcgaccacg tacttgctgc ggacctcagt tcctaacaga
     1441 cttgagttcg tcagctcctc gagcttgtct gcacagtcac gacagccgca ccagtgaaca
     1501 accactacgt ttccatcgag tttctcgtta cactcttcag gagttacagc agtcatcaga
     1561 tggctttcca gatgattttc cgcacgttca aggatttgat cgtgggcttc gtcaaggagt
     1621 ttctttaccg actctgcagc attttcacgt ggaataaccg ttttcacacc aagacgattc
     1681 acacagacaa gctggttgtt gtcaagatca cggggaccga gctcaacacg gagcgggaca
     1741 ccgtgcagtt cccagtggta atattttgca ccgggacgca tgtcacggga atcagtcttc
     1801 acgcgaagac cggcagattt cagttcactc tccagagttt ttactgcagc aagaatctca
     1861 tcaccgcgct ttcccacgat gaccggaatg ataacaacct gtgttggggc gaccgttgcc
     1921 ggaagtacca gtcctttgtc atccccgtgc acaccaatga ttgcagcaat acacctctcg
     1981 gagataccgt aacaggtctg tgatccaaat tgctgctcac cattcacatc ctcataggtg
     2041 atgccaaacg tcttggagaa gtgatctcca agatggtgga cagttcctat ctggagtgtg
     2101 cgtccatccg gcattacagc atcaatcgcc atggtaaagt cagctcccgg gaacttatcc
     2161 cagtccggac gcttggagat gatgattgga acgccgagat cgcgataaaa ttcctgagag
     2221 agagcaagtt catactcaac ctgctcattc gcctcatccc aggttgcgtg gactgtgtga
     2281 gactccatga aagaggtgat ctcacgaagc cggatgagcg gacgtgtgtg ctttgtctca
     2341 taccggaagg tgttcacgat ctgatagagt ttcagcggga gatcagcatg agaccggatc
     2401 cacagcgcat acattggata aattgctgtt tcagaggttg gacggagggc gagtttcaca
     2461 tccagagggg agagaccgcc atgagtaacc cagtacacct catcctcaaa acctttgatg
     2521 tgttccgcct ctttcataaa ctccgtctcg ggaatcagga gcgggaacag tgtctcttca
     2581 tgatctctgt ttaagaggct gcgaagcagt gtgtaagtat ggttgcggag tgcaaatcca
     2641 aacgggtacc acacatacag accttttacc gggtagcgga catccataat ctccgcgcgc
     2701 cgaataactt cattgtacca cgcgctgaaa tcacttcgcg gcggtaatcc ttccgtatca
     2761 tctgccatgt tgtatataca cctgaagcag tagatctatg cgtcgagaga gtattaagat
     2821 aaccaacagg atttgtccaa aaaattggaa ctcatacagg agaagtacac acgtcacgtt
     2881 atggttttga agtacaacag ttggaacagg agcgtagcga aacaataacc acagggaagg
     2941 gcagaatcaa atctcaagaa ccgatcgtcc gccgccggta atacactcac ctccggatct
     3001 tccgaccaca taggtatcct caacgccgac cgtcccgact ccaggaacac cggattttgg
     3061 ttcaagtgca ataaccatat tctctgtaag cggtgaatca aaacccagcg caatcaccgg
     3121 ccattcgttt accacaagac caataccgtg accaaggaag gacacacggc ggcttttata
     3181 ccccatgaaa ttctcggaga gctctggact gatagagtta agaatctctg cataaatttc
     3241 agatggaatg tttcctgcgg caaggaggga tgcagccttt ctctgcactg caatacactc
     3301 ttcatgcagc ttcagggcat ggtccggcag ttttccctga aaggagtaga cctgtgtttt
     3361 atctgtgtga tatccattgt acccatagcc gatatcaaca aacacgagat ctccttttgc
     3421 aagcagacga tctctgctac ctaccgaggg gacaatcgca gatgcaccca gcattccccc
     3481 agggccgtca aagcaggttg cttcaagggt cgttgtgcca aaacccatct gaccgatctg
     3541 catctctgtt ccaaaaccgg aaaaccgtac cactccttga tagtgcagat ccatcattgc
     3601 gtgatagagt tccccaaata actgtgcctc actcatcccc tcatacaaaa tctccggcac
     3661 aatcttcatc agaaggtgat cgtgttccct ccctgcacac cgcagaaact ccagttcata
     3721 ctgacttttc accgcacgga gactgagcat caccgggtca agggcaagca ctgtctcgaa
     3781 ccggaagtac tttttcaacc ggtcaagtac agctaccggg acctgatccg tttcaacaaa
     3841 gattctcccc aaatccgagg ggagatagtc cctcatatcg cggtagcttt tcattggata
     3901 aacagtacca agcggggact cagcctgtgc ccgctcgaaa ctccttcgaa caaaaaacag
     3961 cacatccccg tttctgcgta aaacaagaac accgtcctga atcgttccag tgagatagta
     4021 catattgatt cttccaaaaa tgagggctgt gtcccaggac gagtcagaca tttcaagtgc
     4081 ggagaagagg tgtgcaagcc gcatctccga ctcagattta ggaagtttca gatacacgaa
     4141 aaatctccat ccgtaaaatt actgatgtga tatgttgcac cagagattaa tactgaggga
     4201 ttagggagat gtcctcagaa attacccact ctatggtttg tagacgaaat cagacacctg
     4261 aaagaacgag cattgcaatc tccggcgtca caaaggactc taccaatgct gcaatgacca
     4321 aaagcggaac aacaacaagc aaaaaacgtg tcccatacca tagacatgtt tttccagcat
     4381 tgtctcttcc ctgtgcatcc agataaagag aacgtcctgt ctgcagacca agtgctgcag
     4441 ccatgagaat tgctgcaatc tcaaaaattc cgtgagggac aagtgttgcc gcaaaaactg
     4501 atacatcata cccgcgcagc ataacacttg agatgctacc gatcacatag ccatttgaca
     4561 ccagaacaaa cagggtgacg accccaaacg tcactccacc gataaacagg attaagcaga
     4621 ccttcagatt attgaaaaag atctgccagg acagcaatgc aggcatcgag gaatccagtt
     4681 ctgtagtaga gctctggaat gtcacaacaa tctcgtcagc catagccggc atctcgatgg
     4741 actcggcata cccatatata caggagccaa agaataccac cacaacaacc gcaagtgcaa
     4801 tgatatagag acatctatct gacaacatca gatgaacagc accatccgca gaatatcccg
     4861 aacgcccggt gcaaatccga ccgtgatcat agcaagcagt accagatgcc agacctcatc
     4921 cataccctct tcgttccgga acttctccag aacccagatg gcagggatca ggactaccat
     4981 tttgagagga aacatcacaa atgccgttcc cgtaagctgg ataagtgcat ctcccaagac
     5041 gtgctgttca atatagtgca tcggatgcag atccagtgca aaacttgtgg ccgacgcatc
     5101 cagaagctga ccaaatatca gaatcttgta cagtggatgt gtcacgtatt cccaggaaaa
     5161 accatagcgg agtaaggccc agaacagaga cgccacaacc gttgccatca ccagaataca
     5221 tcctgcaacc cacagcgcaa gctccgtctg agtgattccc caccagcaaa ggaacactaa
     5281 acacgcaaga tttgatatga caccaaccca gaaatacggt tttgtatagg aggcgacaat
     5341 cccgtgattt tccaaggtgc gggagagtac cagaacgaaa atcgtgtaca acaacaccag
     5401 gaaaaagatt ggaggtgtaa caaacaacac ccaccatggc tcggggataa atccggtatc
     5461 ctccacaacc cggagtactc cgccgaagat gacaaacggc atcgatgcca agataagctg
     5521 cgtatcaacc ctgatgttat tcgcccgtgc ccagcggtaa agaagataaa gggtgacgag
     5581 taacaagatt gcacacgtaa tcgtcagata gatattgtac ggctggtcct caaggactgc
     5641 atcgatatag tatttttgta tgaacgcacc tatttcatta aacatatggg ggtcccgacc
     5701 gtgaacgtag acaataatag gatactaaag gataaaacct ttgacaaatc ccgttggatt
     5761 cagatgccac gcaatgtcgt ggtcggccat gacgcactac tgcaaatccc aggaattatc
     5821 gacgacctcg gtgttgcagg acccatcatg ctcctgtcag gccctacaac tatgcagacc
     5881 gttggcgcac gggttgcaga gctcctagtg gggcactcta tcaccacctg ccttgttgga
     5941 cgaatcacct atgatgagat tcaacgcatt gaagacgagg caaagaaaac aggagtcgtt
     6001 ctcatcatcg cagtaggggg aggacgtgtc attgacactg caaaagttgt ttcctataat
     6061 cttgacattc agtttgtcag tgtccctacg gcagcatccc atgatggtat tgcatcttcc
     6121 cgcgcttcgg ttgtaacgga ttctgggaat gtcagtgttg cagcccaacc accgcttgca
     6181 attgtagctg atacaggtat cattgcaacc gccccgcacc gactgcttgc tgcaggatgt
     6241 gcagacatca ttgcaaatta tactgctatt cttgactggg agctctccca tcgtcttacg
     6301 ggtgagacaa ttagcgagta tgcgctgacc ctctcaaaga tcactgcaga gattctggta
     6361 aacaacacag atcttgtcag ccacaatgat gaaactgctg tgtggattgt agtaaaggca
     6421 cttttttcct cagggattgc catgagcatt gccggaagtt cccgccctgc atccggcggt
     6481 gaacacaagt ttgcccacat gcttgaacgt cttgcaccgg gatcagcttt gcacggtgag
     6541 gcttgtggac tcggtactat cattggcatg tatcttcacg gaggggattg gaaaggtatc
     6601 cggacctcac tcaaacgcat cggggccccg accacaccac aggaccttgg tattagtaat
     6661 gagatctgcg ttgaggcagt cctcagggcc tgtgagatcc gacccgaacg attcacaatc
     6721 tttgacacag gaatcacgag agaagcagcg gttgcggcag tagaagcatt gtatgaggtg
     6781 taatacagaa tggacgatac agaaaaaata gtcaccatcg ttggatccgt gcttgcccac
     6841 gagggagccg agttcgtcta tgcgggaaag ataaccgaat gtgataattg taaagtcggc
     6901 aaagtatgcc acaatgcaaa actccgggaa ggacgcagat accgtgtgat tgcagttcgt
     6961 ccaacaaaac acgaatgctt tgttcatgcc ggcggagcag ttgcagtgga ggtggcagaa
     7021 gcgccaatca ctgcggtaat tccaacaagc caggcaaccc gccggacccg aattacttac
     7081 acaccagttt gtgacgatcg gttctgtaaa ggctatgcat tttgccatcc tgatgggatg
     7141 acagacaaag gacgttatgt tgtgcttgag gttcttggat cctacaatga aatgtgtccg
     7201 atgggaaaga aaaatctcaa actcgttgag ctgcgccacg tgccaacata agggcattgg
     7261 cacactcagt gatctttttt cggatattga tccggcaaac ccattccgtt ggcagatcgt
     7321 ttatgccgaa gtacagtcca gcaagtcccc cggagataga tgcaattgta tccgtgtcac
     7381 cgccaagtgt taccgcgcat tcaatacagt cacgaaccga ctcagagtca cggaaacagg
     7441 aaaacgcaca tcgtgttgcc gccactgcat cgaccgaggg tatgagggct ccggaaaaca
     7501 aatccgatct ccctgatgca tcccatgctg ctgcaagagc ttcatctttg tcaccccctg
     7561 ccaataatac tgcggccgca gcggaaaccg ctgcacagca gtctcctgca atcggatcat
     7621 agtgggtgaa cgccgacact cgccgcgctt cagaggctac cgtttcagga gtacagatca
     7681 ttcctactgg aagcgttcgc atgacactgc catttgttct gctcccaaaa agcaagtcta
     7741 ccactttaac tgcctcatca gaaacgcatc cctgttccaa aagtacacat agtgtctttg
     7801 tggtcctgcc gaatgttact ggattctgca gaacaaccct cgtcatacgc aggagaaatg
     7861 cctcccgatc aaacgtacca taggaaagat acgtttcaag cagggaaagt gtcatcattg
     7921 tgtcatcggt taccgcaccg gcaggaaggg aaaattgtcc gcccccagta accgagaggt
     7981 tgtaacgtgg ccctggcatc attccctcaa aggtaagacc aaccgcatca cctgctgcca
     8041 caccgaacag ccctccaaag atcctttcct tctcagatat aaacgtcatc caaaccctca
     8101 tatgtgagtg atgtactccc aatagaaaac ccggttcgca caggttattt tgtaaaatta
     8161 cgcttgtagc aagcagaact aagtcacaca cctggggaac taaaccggga gtttcttctc
     8221 agatataaac gtcaccaata ac
//
LOCUS       DALUAJ010000144         1956 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_25793,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000144 DALUAJ010000000
VERSION     DALUAJ010000144.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25793"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="O0X37_03100"
     CDS             complement(<1..435)
                     /locus_tag="O0X37_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HJK61946.1"
                     /translation="MTRNSIEAEVLSRVLPTDAERAAAQAMGEKLIAAVYEAANVPAM
                     MTGSVARGTWVRGDKDIDIFMLFPPELSREELQEKGLAAAYAVVEKFSGTAEEKYAEH
                     PYLNAVIEGFDVDLVPCYHVASTAEMKCAVDRTPFHTRYLLPK"
     gene            complement(432..971)
                     /gene="thpR"
                     /locus_tag="O0X37_03105"
     CDS             complement(432..971)
                     /gene="thpR"
                     /locus_tag="O0X37_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HJK61947.1"
                     /translation="MVRTFIAVEPSDEIKTALADAGRSLLGTPARITAVQASLMHITL
                     KLLGEVPESQLPKIRTALSGIHAAPYQITVAGAGVFGRPPRVIKAEIEDCGATADLAR
                     QIDTLLQPLGIPRDPKPFSPHLTIARVREYSPALLPKIAALKQQPSFGTCTIGEVLLK
                     KSTLTPSGPIYETIGGVRL"
     gene            complement(1023..1826)
                     /gene="folP"
                     /locus_tag="O0X37_03110"
     CDS             complement(1023..1826)
                     /gene="folP"
                     /locus_tag="O0X37_03110"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HJK61948.1"
                     /translation="MEVRIQHLCIGNSSSPKIMGVLNISPESFFSDSFTPCDQVVTRV
                     EEMIREGADIIDLGARSTALNAPPLTVAEERDRVITAMKELDGYGAVLSLDTMYPEVL
                     DAALHYDLAAINDINGLGNPAYARLAADSGLPVIAMAAHKLPGDPLDRAGTHAAMQEI
                     LDRAGQYGITDIILDPGVGKWVADRSTAADWDLCRQFAELKRYGCPLLAAVSRKTFIG
                     DCIGKPAHERLYGTLGVLFYLLENGADLIRVHDVGAARDVVRVFEQLCR"
BASE COUNT          412 a          516 c          589 g          439 t
ORIGIN      
        1 cttggggagg aggtagcggg tgtgaaacgg cgttcgatca accgcacatt tcatctcggc
       61 agttgaggca acatggtagc aggggacgag atccacatcg aagccttcga ttacggcgtt
      121 gagatagggg tgttcggcat acttttcctc ggcggttccg gaaaactttt ccaccactgc
      181 gtaggctgcg gcaagccctt tttcctggag ttcctctcgc gaaagttcgg gggggaagag
      241 catgaagatg tcgatgtcct tgtcgcctct gacccaggtg ccacgcgcaa ccgatccggt
      301 catcattgcg ggaacattgg ctgcctcata gaccgcggcg atgagttttt ctcccattgc
      361 ctgagccgca gcccgttcag catcggtagg gagcacacgg gataacacct ctgcttcgat
      421 cgaatttctg gtcacagtct tactcctcca attgtttcat atataggtcc cgacggggtt
      481 aaggtactct ttttgaggag cacctcgcca atggtacagg ttccaaacga cggctgctgt
      541 tttaaggcgg caatttttgg aagaagggca ggcgagtact ccctgacgcg ggcaatggtc
      601 aggtgcgggg agaagggttt ggggtctctg gggattccca gcggctggag aagcgtgtcg
      661 atctgccgtg cgagatccgc agttgcaccg cagtcctcaa tctctgcttt gataacacgc
      721 ggaggccggc cgaaaacccc tgctcctgca accgttatct gatagggggc agcatgaatg
      781 cccgaaagcg cagttctgat tttgggaagc tgcgactcgg gaacctcgcc aaggagcttg
      841 agcgtaatgt gcatcagaga tgcctgcact gctgttattc tggcaggtgt tccaagaagg
      901 ctgcgccctg catcagcaag agcggttttg atctcatcgg acggctccac cgcaataaat
      961 gtccgcacca tgtatgcgga gtatataggc ggttgaaaaa ataggagttt tcggcgtttg
     1021 ggttaccggc agagctgttc aaagacccgg accacatccc ttgcggcgcc aacgtcatgg
     1081 acgcggatga ggtccgcacc gttttccagc agatagaaca gtacgccaag tgttccgtac
     1141 agacgctcat gcgccggttt tccgatgcag tcgccgatga aggtcttgcg ggagaccgcg
     1201 gcaagcagcg ggcatccgta gcgtttcagt tcggcaaact gccggcagag atcccagtca
     1261 gccgcggttg accggtcagc cacccatttt cccacgccag gatcaaggat gatatcggtg
     1321 atgccgtact gcccggcacg gtcaaggatc tcctgcatgg cagcatgggt tccggctctg
     1381 tcaagcgggt ctccggggag tttgtgtgct gccatggcga tcaccggcag gccggagtct
     1441 gcggcaagtc tggcatatgc agggtttccc agaccattga tgtcattgat tgcggcaagg
     1501 tcataatgca gggcagcgtc cagtacctcc ggatacatgg tgtcaagcga aagaacggca
     1561 ccgtatccgt caagttcttt cattgcggtg atgacgcggt cccgctcttc cgcaacggtc
     1621 aggggaggcg cgttcagtgc cgtgctgcgg gcacccagat ctattatgtc ggctccctcg
     1681 cggatcatct cctcaacgcg ggttaccacc tgatcacagg gagtaaacga gtctgaaaaa
     1741 aaggattcgg ggctgatgtt gagaaccccc atgatctttg gcgaggagga gtttccgatg
     1801 caaagatgct gaatccggac ttccatttca gatcgcccaa atgcctttat tcccgaatct
     1861 ccgggtagag cggatagcgc agacagaagg tggtgacttc ctcgcgggtt tctgcaatca
     1921 gatcggcatc gtcgatgtgg tggagcacac gggaga
//
LOCUS       DALUAJ010000145         4344 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_25855,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000145 DALUAJ010000000
VERSION     DALUAJ010000145.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25855"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="O0X37_03115"
     CDS             complement(<1..170)
                     /locus_tag="O0X37_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0147 family protein"
                     /protein_id="HJK61949.1"
                     /translation="MASPEEMIKQCVMMLQAISEDSTIPRNIRRVADETKTILLDEKK
                     TTGLRAADALSL"
     gene            269..721
                     /locus_tag="O0X37_03120"
     CDS             269..721
                     /locus_tag="O0X37_03120"
                     /inference="COORDINATES: protein motif:HMM:NF018392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61950.1"
                     /translation="MLSICDEQTNSITMTNGARKPDDIMAEYLLGGAKMLADLCPKCG
                     APMFEVKGNRMCVVCAEKAATSQTETPKVVPANGAESLPEKVRVITPKYAKPQSTPTP
                     VSSDIPARLDALILQFCARAENEPDPARCLSYMECIRTAAEARTMLNR"
     gene            763..1302
                     /locus_tag="O0X37_03125"
     CDS             763..1302
                     /locus_tag="O0X37_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HJK61951.1"
                     /translation="MKVIGVVGYPASGKGEFSQIAAGLGIPVVVMGDIIRRQVSAQGL
                     ALTDENIGATARKLREDFGMDAVAMLTAEEVGKQHTDVVVIDGIRGDAEVRYFRSVFP
                     TFVLVAVEASFPVRLSRMQVRGRSDDTTTAESLKGRDEREESFGLATAMTLAEVRLVN
                     ESTRDVYEAAVRKVLSEEL"
     gene            1299..2078
                     /locus_tag="O0X37_03130"
     CDS             1299..2078
                     /locus_tag="O0X37_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HJK61952.1"
                     /translation="MKIYFASSRLSLKDPESWVASIAEAGFDGWEISMDGWFHSVEDI
                     PKKFPGIKKVLKETGLEASVHLPFSGLNPASLNTDVWSATVSQLSACIEAASEIADTI
                     TLHPGYLEPNGREATSAAWNAHREALEKFGEAAEHSGVCVALENMPNLEDFYCRDPYE
                     LAGFADAVSGISLTFDVGHANTNGNLDTFCKVILPRAAHMHIHDNHGKYDEHLPLGKG
                     SIDWKRVMPKIRAEYRGKIMVVEGRNPAEGRISLDLIREWF"
     gene            2081..3022
                     /locus_tag="O0X37_03135"
     CDS             2081..3022
                     /locus_tag="O0X37_03135"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HJK61953.1"
                     /translation="MAGGETLFVHFLGTAGALPTPNRNPSCIMIRRGSDTLLFDCGEG
                     AQQQMMRAKTGFTVNAIFVTHWHADHYLGIFGLVETMAFNGRTEPLTIYGPAGVEGFV
                     EIIHRLTPKIPFVLSAVEVADGDVTLFDGYSVMAFKTYHGMESVGYVLEEDMRPGRFN
                     REGAIALGVPPGPLFGKLQRGESVRIVRNGEEVTISPEQVMGERRRGRKVVYTGDTRP
                     VKNKNELLEGADLLIHEATFDEEEGSARAKEVWHSTAREAGMVAAAVRPKILALVHFS
                     SRYTSTANHIQDAKEFFDGEVVAPADLSVIEIPFSDV"
     gene            complement(3096..>4344)
                     /locus_tag="O0X37_03140"
     CDS             complement(3096..>4344)
                     /locus_tag="O0X37_03140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK61954.1"
                     /translation="AVEGVGNALMFGTAIALVTSVVNAELRGTAIGIAMTGVFLGQLA
                     GPLLAGALTDVYGWQMVYLILVPIALLSFVLAVPFIPRDAPTDKMPYDWVGAVLFILG
                     MTLGLYGFSKMPDVLALVLLVCGLLLLGIFFWYEMRAANPLIPVQIILKNRRFTFNNS
                     ANLLYYVAIYAMGSLVSMYMTHAWNFSALDRALVVTTQGLILVLFTIVTGKFYDHLLP
                     RWLILSGVGMAVVGGAAAYLLGVPSADPVWLLAAVIAASIAAFGCGSIVLAVIDRMVK
                     NAPPRYATTTGLFIIALGMVLLLTCGAEQSVWTMIAVQALFGVGIAIFVTPNSTAIMN
                     AVSEQEYGMASGTLSTTRMLGMAVSIGMMTVLTNLFLGAGVQMGPEYVEEFLVMMHAT
                     VLAAIAVLVIAMILSWTADSRTS"
BASE COUNT         1139 a         1087 c         1220 g          898 t
ORIGIN      
        1 attaaggaca gggcatctgc tgcccgcagt ccggttgtct tcttttcatc aagaagaatg
       61 gtctttgtct catctgcgac acggcggatg ttgcggggaa tcgtcgagtc ctcgctgatt
      121 gcctgaagca tcatcacaca ctgttttatc atctcttccg gacttgccat cttgaaaatc
      181 cctcctgaaa gcatgaccta tcctaataga acaggatagc attacctcaa aactatgctt
      241 tagattagag cgtgcacaaa gataaaggat gctatctata tgtgacgaac agactaatag
      301 tattacaatg acaaacggtg cacgaaaacc tgacgatatc atggccgagt atcttctcgg
      361 aggggcaaag atgctggcgg atctgtgccc aaagtgcggg gcaccaatgt ttgaggtgaa
      421 aggcaaccgt atgtgtgtgg tctgtgccga gaaagcagca acctcacaga cagagactcc
      481 aaaagtggtc ccggcaaacg gagctgagtc actccctgag aaggtacggg tgattacccc
      541 caagtatgcc aaaccgcaga gcacaccaac tccggtttcc agtgacattc ccgcaagact
      601 ggatgctctg atactgcagt tctgcgcccg ggctgagaat gaaccggatc cggcacgatg
      661 tctatcatat atggagtgta tcagaacagc agcagaggcc cggacaatgc tgaaccgctg
      721 aaagcgatgg gcttaatgaa attccctgca tatgaattac agatgaaagt aatcggtgtt
      781 gtcggatatc cggcgagcgg aaagggtgaa ttttcgcaga ttgccgcggg acttggcatt
      841 cctgtcgtgg taatgggaga tattatccgg cggcaggtaa gtgcccaggg ccttgctctc
      901 accgatgaga atatcggagc cactgcacgc aaacttcgcg aagatttcgg gatggatgcg
      961 gtcgcaatgc tgaccgctga agaggttgga aaacagcata cggatgtggt ggttattgac
     1021 ggaatccggg gagatgcaga ggtgcggtac ttccggtcag tattcccgac gtttgtgctg
     1081 gtagcagttg aagcttcgtt tccggtacgg ctttcccgaa tgcaggtacg ggggagaagc
     1141 gatgacacca caacagcaga gagcctgaaa ggccgcgatg agcgcgagga gtccttcggt
     1201 ctcgcaaccg cgatgacact tgccgaagta cggctcgtga atgagtcgac acgggatgtg
     1261 tatgaggcag cggtgcgcaa ggtgctttcg gaggagttat gaagatttat tttgcttcat
     1321 cacggctttc cctaaaagat ccggaaagct gggttgccag tattgccgag gcggggtttg
     1381 acggatggga gatctccatg gacggatggt tccacagtgt tgaggatatc cccaagaaat
     1441 ttccggggat caaaaaagtc ctgaaagaga cagggcttga ggcctcggta catctgccgt
     1501 tttcaggcct gaacccggca tcactaaaca cggatgtgtg gagcgcaacg gtgagtcagc
     1561 tctccgcatg tattgaggcc gcatctgaga ttgcagacac cattaccctg catccgggat
     1621 accttgaacc aaacggccgc gaggcgacat cagccgcatg gaatgcccac agagaggcgc
     1681 tggaaaagtt tggagaggca gcagaacatt cgggagtctg cgtagcactg gagaatatgc
     1741 cgaaccttga ggacttttac tgccgggacc catatgagct ggcaggtttt gcggatgcag
     1801 tttccggaat ctccctgaca tttgatgttg gtcatgcaaa cacaaacgga aatctggaca
     1861 ccttctgtaa agtgattctg ccgcgggcgg cacatatgca catccatgac aaccacggga
     1921 agtacgatga gcatctcccg cttgggaaag gatcaatcga ctggaagagg gtcatgccaa
     1981 agattcgggc agagtaccgg ggaaagatta tggttgtgga agggaggaat cctgccgagg
     2041 gcaggatcag tctagatctg atcagggagt ggttctgagt atggctggcg gagagacgct
     2101 gtttgtacat tttctgggaa ctgctggtgc gctcccgaca ccaaacagga atccttcctg
     2161 tattatgatc cgccgaggtt cagacacact tctgttcgac tgtggggagg gagcccagca
     2221 gcagatgatg cgggcaaaga ccgggtttac ggtaaatgcg atctttgtaa cgcactggca
     2281 tgcagatcac tatcttggga tattcgggct ggtggagacc atggcattca acggcagaac
     2341 agagccgctg accatctatg gacctgccgg agtggaaggg tttgtggaga tcattcaccg
     2401 gctgacacca aagattcctt tcgtattgtc tgcggtggaa gtagctgacg gtgatgtaac
     2461 actgtttgac ggctactcgg tgatggcgtt taagacatat catgggatgg agagtgttgg
     2521 gtatgtgctg gaggaggata tgcgtcccgg acggtttaac cgcgaaggag caatcgctct
     2581 cggagttcct cctggcccgc tgtttggaaa actgcagcgc ggggagagtg tacgaatcgt
     2641 ccgtaacgga gaggaggtca caatctctcc ggaacaggtg atgggcgagc gccgccgcgg
     2701 cagaaaggtg gtgtacaccg gcgacacacg gcctgtgaag aacaaaaacg aactgctgga
     2761 aggagcggat ctactgatcc acgaggcaac atttgacgag gaggagggat cagcccgcgc
     2821 aaaggaggtc tggcactcaa ccgcacggga ggctggaatg gttgccgcag cggttcgtcc
     2881 gaagattctg gcactggtgc atttcagttc gcggtacacg tcaacggcaa accacataca
     2941 ggatgcaaag gagttctttg acggagaagt tgttgcgccg gctgatctgt ccgtgattga
     3001 gattccgttc tcggatgtct aagctgggct tccagtccac agaaaaaaac atcatactgg
     3061 ataccatatc cagaaaatct ctttttagaa aaaaattagg atgtccggga gtctgcggtc
     3121 caggatagaa tcattgcgat aaccagcacc gcaatcgccg cgagaactgt tgcatgcatc
     3181 atcaccagaa actcttcaac atactccggc cccatctgca caccggcacc gaggaacagg
     3241 ttggtaagga ccgtcatcat cccgatcgag actgccatcc caagcatccg cgtcgtcgag
     3301 agtgtgcccg atgccatgcc gtactcctgc tcgctcaccg cgttcatgat cgcggtactg
     3361 tttggagtca caaagattgc aatacccaca ccaaacagtg cctgaaccgc aatcatcgtc
     3421 cagacagact gttctgcccc gcaggtcagg agaagcacca tgccgagtgc aataataaac
     3481 agaccggttg tcgtcgcata gcggggaggc gcatttttca ccatgcggtc aattaccgca
     3541 agcacaattg atccgcagcc aaacgctgcg atacttgctg caatcaccgc tgcaagcagc
     3601 cagacagggt ccgcagacgg aacaccaagc agatatgccg cagcaccgcc gacaactgcc
     3661 atacccacac cagacagaat caaccagcgc ggcagcagat ggtcgtagaa cttgccggtc
     3721 acgatcgtaa acagaacaag aatcagtccc tgcgttgtta ccaccagcgc acgatccaga
     3781 gccgagaagt tccaggcatg ggtcatatac atcgagacca gcgaacccat tgcataaatt
     3841 gccacataat acagaagatt tgccgagtta ttgaacgtaa atctacggtt cttcaggata
     3901 atctgtacgg gaataagcgg gtttgccgca cgcatttcgt accagaaaaa gatgccaagc
     3961 agcagaagac cgcaaaccag cagcaccagt gcaagtacgt caggcatctt ggaaaagccg
     4021 tagagtccaa gtgtcatgcc aagtataaac aaaaccgccc ccacccagtc atacggcatc
     4081 ttatccgttg gtgcgtcacg cgggatgaag ggaactgcca agacaaatga cagaagggca
     4141 atcggtacca gaatcagata caccatctgc catccgtata catccgtcag agcaccggcg
     4201 agaagggggc cggcaagctg tccgagaaac acaccggtca tggcaatacc gatcgccgta
     4261 ccccgcagtt cagcattcac cacagacgtg accagggcaa ttgctgtgcc aaacatcaac
     4321 gcgttgccga caccctcaac cgcc
//
LOCUS       DALUAJ010000146         2343 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_26242,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000146 DALUAJ010000000
VERSION     DALUAJ010000146.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26242"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="O0X37_03145"
     CDS             <1..1508
                     /locus_tag="O0X37_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61955.1"
                     /translation="TVFSTNWCGGVLLGAAGTAYTGYVVSTIADPWATCLTLVLMAIF
                     FCAILRKLKIASLSEMYRLRFGKRGATVATVMCIPSMITWTAANIVALTTIFKLFLDF
                     DPLICAIIAGLIVVLYTYLGGMLAVVYTDNIQAVMIMLGLIILIPTGIAFVGGFDVLA
                     AATPENFWNLLPNDGAGAVATGFTVDPLGIFTWLAGLSGMGFGYLASVELTQRVLCAR
                     DTKAARQGLLIGSGMYALAGFFSMMIALIAIVMVSQGTTTPAGVPMGEILAEDGNYVL
                     LVLAERLFDPSVMGIIGVVLMAIFIGSLLAAIMSTSSSTIFAASAALSTLMLHGSLSK
                     YATTSLQVLRLTRMLVLVIGVFCVFFSFVIDSLYWMMIFSFTILFNCMFWALVGGLFW
                     KRCNASAAIASIVVGFAGVILCIIAQSLYFGQIAIEPQDNLWIGIQTFVPWAMGGIAM
                     LITALLTQKSDPPVALCDTDGELVKWEDLPLSADTIEVRRRIEEIKAREEE"
     gene            complement(1598..>2343)
                     /locus_tag="O0X37_03150"
     CDS             complement(1598..>2343)
                     /locus_tag="O0X37_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HJK61956.1"
                     /translation="DRLIIADRGYQKGIEDQIRKAHAAGWKIEWDDHHKGSEEEMERV
                     RPFVISLTVDTSVCATGVVCRAFAQGNPVATEVARVVCDYDLWKHEDSRSAVLGMVTS
                     KREYLELVRNKLTEGIIIDDEISEIFAEIERDKNRCMQKSIRRAKIFRGKYTIAVMPA
                     YGYPSETAAEARKELGCDMELLVFDTGKFSLRSVAPVSHLIAKQFNGGGHPNASGGSF
                     AYGWKEKWMLKLFGRVSCAKEFAKTAELV"
BASE COUNT          562 a          574 c          548 g          659 t
ORIGIN      
        1 gcacggtctt ctccacgaac tggtgtggcg gagtgctact gggagctgcc ggtacagcat
       61 acacaggata tgtggtgtcc acgattgctg atccatgggc aacctgtctg acccttgtac
      121 tgatggcaat attcttctgt gcaattctgc gcaaattaaa gattgcatct ctgtcagaga
      181 tgtatcggct gagattcgga aaacgcgggg caacagttgc aacggtaatg tgcattccgt
      241 cgatgatcac gtggaccgcg gcaaacatag ttgcactgac aacgattttc aagctgtttt
      301 tagattttga tcccctgatc tgtgcaatta ttgccgggtt gattgttgta ttgtacacgt
      361 atctcggtgg aatgcttgcg gttgtgtata cagataacat ccaggcggtg atgatcatgc
      421 ttggtcttat tattctgatt ccaaccggaa ttgcgtttgt cggaggattt gatgtactag
      481 ctgcagcaac gcctgagaac ttctggaacc ttctgccaaa tgacggagcg ggagcagttg
      541 caacaggctt cactgtggat ccgcttggaa tctttacatg gcttgcaggc ctatctggaa
      601 tgggatttgg ataccttgca tcagttgagc tgacacagcg ggttttgtgt gcccgtgaca
      661 cgaaggctgc acgtcagggc ctgctcatcg gatcaggcat gtacgcactt gcagggttct
      721 tctcgatgat gattgcactg atcgcaattg tgatggtttc acagggtaca acaacgcccg
      781 cgggcgtccc tatgggagaa attcttgcag aagacggaaa ttatgtactg ttagtacttg
      841 cagagcgctt gtttgatcca agcgtgatgg gaatcattgg cgttgtattg atggcgatat
      901 tcattggatc tctccttgca gcaattatgt ctacatcaag ttcgacaatc tttgccgcct
      961 ctgcagctct ttcaacactg atgctgcacg gatcactgtc gaagtatgca acgacctcat
     1021 tacaggtact tcggctgaca cgtatgctgg tactggtgat aggagtattc tgtgtattct
     1081 tcagctttgt gatagacagt ctgtactgga tgatgatctt ttcgtttacg atcctgttca
     1141 actgcatgtt ctgggcactt gttggcggcc tgttctggaa acggtgtaat gcatcagctg
     1201 caattgcctc aattgttgta ggttttgctg gggtaattct ctgtattatt gcccagtcgc
     1261 tctacttcgg ccagattgca atcgagccgc aggacaacct gtggattggt atccagacct
     1321 ttgttccgtg ggcaatgggt ggaattgcaa tgctgattac agcactcctt acacaaaaat
     1381 cagatcctcc agtggcattg tgtgatacgg atggagaact ggtaaaatgg gaagatcttc
     1441 cgctttcagc agatacgatt gaggttcgca gacgtatcga ggagattaaa gcacgagaag
     1501 aggagtaaat ttcctttttg gatttctttt ttgcaacaac cctaaaaatg tactaaaaaa
     1561 ggaatggtgc agattacact gcattttcct gaaaaaatta tactaactca gcagtcttgg
     1621 caaactcctt tgcacaggag acacgtccga acagtttcag catccacttc tctttccacc
     1681 cgtacgcaaa actcccaccc gatgcatttg ggtgtccacc cccgttaaac tgtttggcaa
     1741 tcagatggct cacaggtgca acgcttcgga gagagaactt gccggtatca aataccagta
     1801 actccatatc acacccgagt tcttttctcg cttctgctgc agtttcacta ggataaccat
     1861 aggcaggcat taccgcaatc gtgtactttc cccggaatat ctttgcacgt cggatgctct
     1921 tttgcataca cctgttctta tctcgctcga tctccgcaaa aatttcagag atctcatcat
     1981 caatgataat cccctctgtc agcttattcc ggaccagctc aaggtactcc ctctttgagg
     2041 tcaccattcc caaaactgcg gaacgcgaat cctcatgttt ccacaaatca taatcacaca
     2101 caacccgcgc aacctctgtg gccacaggat ttccctgggc aaatgcacga caaacaacac
     2161 cagtggcaca gacagatgta tccacggtaa gagagatcac aaacggccgg acgcgttcca
     2221 tctcctcttc cgaccctttg tgatgatcat cccattcaat tttccatcct gccgcgtgcg
     2281 ccttgcggat ctgatcctca atccctttct gatacccacg atccgcaatg atcagacgat
     2341 ccc
//
LOCUS       DALUAJ010000147         3895 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_26679,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000147 DALUAJ010000000
VERSION     DALUAJ010000147.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26679"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /gene="fdhF"
                     /locus_tag="O0X37_03155"
     CDS             complement(<1..1616)
                     /gene="fdhF"
                     /locus_tag="O0X37_03155"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HJK61957.1"
                     /translation="MEIKYVTTTCPYCGAGCTFNLVVKDGKISGVQPCQRGPVNEGKL
                     CPKGIFGWEFIVSEDRLKTPLIKDKATGEFKPATWDEALTVIAENFKKYSPEEIAVIS
                     SARCCNEDNYAMQKFARIVLKTPNVDHCARLCHAPTVAGLNMVFGSGASTNSFDDLAI
                     ADFVFIIGSNNFEAHPLAGRRIMQAKEKGAHILVCDPRVTPTAKQADLHIQHYPGTDI
                     QLLNCLMKQIIERGWVDEEFVKNRTNGYEELKACVTQDKYSIENTSVVCGVPAEKIEQ
                     ALEWLHECQHKTAFVHCLGITQHTVGVDNVRSIAFVQTLLGNIGKPGCGVNALRGQNN
                     VQGSCDMGALPNVYAGYLKVTDPEAAKKVAAYWGIDEIPNGKLGLHIPEMLETFEEHP
                     DKLKCLYLMGENPVMSDPNSKGVEQAMKNCEFLVVQDIFETETTKYADVVLPGSCYAE
                     EDGAQTNAERRVQRFRKAADAPGESKLDWEIMKMIAEKMGYGQYFQWQTSEDVFNEMR
                     GITPQYAGITYAKLEGDGIQWPCPTEDHPGT"
     gene            complement(1897..3255)
                     /gene="cfbB"
                     /locus_tag="O0X37_03160"
     CDS             complement(1897..3255)
                     /gene="cfbB"
                     /locus_tag="O0X37_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide synthase"
                     /protein_id="HJK61958.1"
                     /translation="MRSLLISGDRSGAGKTSITLAIAGLLAQDSPVQTYKVAMDYIDT
                     SYLAGVTGRPSYNLDTFVQTPEETSGLFSYGSVGAEYGIVEGVRGLYEGVDALTDVGS
                     TASVAKMFDLPVILVINARSITRSAAALVKGFAAFDPDIRIEGVILNNVMGERHIGKA
                     TKAVEYYCGIPVLGAVPRRPEMELAMRHLGLVPYREGSDNAEFHERISFITSVIGEYV
                     DIDRIRETAQDRTPRPNAVCEMLTAQSTPTMRIAVAYDEAFNFYYGELTAVLRSLGAE
                     VVHFSPIHDRLPDADGYIFGGGYPELFLEELEHNDAMREAVRETAENNVPIYAECGGL
                     MYLTRSLTLEAGWQGRDDRISCEMCGVFAGDTCMPVRKALGYVIGTASLPSGDFAFRG
                     HEFHYSGISMDAGVHYAYRLSRGTGIQNGCDGALVHQTLGAYTHLMPVSSRSMLKSFF
                     GL"
     gene            complement(3252..>3895)
                     /locus_tag="O0X37_03165"
     CDS             complement(3252..>3895)
                     /locus_tag="O0X37_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni-sirohydrochlorin a,c-diamide reductive
                     cyclase catalytic subunit"
                     /protein_id="HJK61959.1"
                     /translation="SEELERQRRGLAAANATERARGAACKTYISPSRGDLKHVAATEL
                     LSLARSGKRGLAIMNAKKETAYMFADELLALHDTVPDADITYVANLEGRGLPKVRGDA
                     AAILAEMRERGVDPVLCGALDEYGENGSAIAEIIAETHPDFVLLVGVPHAVIPEALQG
                     ITVFSVTNGPRQVAPLKEQGHAYVMVEIDLHPKTLGVHEIVESEFGAVLRSVA"
BASE COUNT          954 a         1069 c          928 g          944 t
ORIGIN      
        1 gttcccgggt ggtcctcagt cgggcacggc cactggatac cgtcaccttc gagttttgca
       61 taggtaatac ctgcatactg cggggtgatt ccacgcatct cgttgaagac atcctcggaa
      121 gtctgccact ggaagtactg gccatagccc atcttttctg cgatcatctt catgatctcc
      181 cagtcaagct tggactcacc aggtgcgtcg gcagcctttc tgaatctctg gacacggcgc
      241 tctgcgttgg tctgggctcc gtcctcttct gcatagcagg agccggggag aacaacatca
      301 gcgtacttcg tggtttcagt ctcaaagata tcctggacaa caaggaactc acagttcttc
      361 attgcctgct cgacaccctt ggagttcggg tcggacataa ccgggttttc tcccatgagg
      421 tagagacact tcagcttgtc agggtgctct tcaaaggtct caagcatttc tgggatgtgt
      481 aatccaagtt tgccgttcgg aatctcgtca attccccagt aggctgcaac cttcttagct
      541 gcttccggat cggtgacttt gaggtaaccg gcgtaaacgt tcgggagtgc tcccatatcg
      601 caggaaccct gcacgttgtt ctgaccgcgg agtgcgttga caccacagcc tggcttgccg
      661 atgttaccga gaagggtctg gacaaaggca atggaacgga cgttgtcgac accaacagtg
      721 tgctgcgtga taccaagaca gtggacaaag gcggtcttgt gctggcactc gtggagccac
      781 tcaagggcct gctcgatctt ttctgccggt acaccacata cgacagaggt gttttcgata
      841 ctgtatttgt cctgagtaac gcaggctttg agttcttcgt aaccgttggt gcggttcttc
      901 acgaactctt cgtcaaccca gccgcgctcg atgatctgct tcatcaggca gttaaggagc
      961 tggatatcgg ttcctgggta gtgctgaatg tgcaggtcag cctgtttggc ggtcggagtg
     1021 acacgcgggt cacagaccag gatgtgggca cccttttcct tagcctgcat gattctgcgg
     1081 cctgcaagcg ggtgggcttc gaagttgttc gaaccaatga taaacacgaa gtctgcgatt
     1141 gcaaggtcgt caaaagagtt ggtcgatgca ccggatccaa agaccatgtt cagaccggcg
     1201 acggtcggtg cgtggcacag acgggcacag tggtcgacgt tcggagtctt taagacaata
     1261 cgggcaaact tctgcattgc gtagttgtct tcgttacagc agcgggcaga ggagataact
     1321 gcgatctctt cggggctgta ttttttaaag ttctcagcaa tgacagtgag agcttcgtcc
     1381 caggttgcgg gcttgaattc accggttgct ttgtctttaa tcagaggggt cttaagacgg
     1441 tcttcggaga cgatgaattc ccagccgaag atacctttcg ggcagagttt accttcgttg
     1501 accggaccgc gctggcacgg ctggacaccg gagatcttac cgtcctttac caccaggtta
     1561 aaggtacagc ctgcaccaca gtaggggcag gtcgtggtta catacttgat ttccatggat
     1621 aaacctcgga tactataacc tctgtaaaga ggacataaat acctatccag atttttctga
     1681 tttttgtgca ttattggtgg gttgaatgtt tttcatcaga actgagaatt caatatgttg
     1741 tgttaataag actatgagat taaacagatg tgcacaataa gcgcgcattt gcggcagatg
     1801 agcgcagatt tatccaaaag caggagtgtc tataaacgaa taggaacctg atctgttcta
     1861 aaaaccggag gagaaagtgg gcactccgaa cagatctcac agtccaaaga aactcttcag
     1921 cattgaccgg gacgataccg gcatcaaatg tgtatatgca cctaacgtct gatgtaccaa
     1981 tgcaccatca catccgtttt gaatacccgt tccacgggaa agtcggtacg catagtgtac
     2041 ccctgcgtcc atcgaaatac cactgtaatg gaactcatgc ccacggaatg caaaatcacc
     2101 actcggaagt gatgcagtcc ctataacata gcctaaagcc ttacgaacgg gcatgcatgt
     2161 atctcccgca aatactccgc acatttcgca tgaaatccga tcgtcgcgac cctgccagcc
     2221 tgcctcaagc gtaagcgacc gcgtcaagta catcagacct ccgcactccg cgtagattgg
     2281 tacattgttc tccgcagtct cgcgtacagc ctcacgcata gcatcattgt gttcaagctc
     2341 ctccaaaaac aactcaggat acccacctcc aaaaatataa ccgtctgcat caggtaagcg
     2401 atcatgaatg ggactgaaat gcacgacctc tgcccccaga cttcgcaaga ccgcagtcag
     2461 ttctccataa tagaaattaa acgcctcatc atatgcaact gcaatgcgca tagtgggagt
     2521 tgattgggca gtgagcattt cgcacaccgc attcggacgc ggcgtcctgt cctgtgcagt
     2581 ttcgcgaatc ctgtcaatat ccacatactc cccaatcaca cttgtaataa aggagatcct
     2641 ctcatggaac tccgcattgt ccgaaccctc acggtaggga acaagaccca gatgccgcat
     2701 tgcaagctcc atctccggcc ttcgcggaac agcccccagg accggaatac cgcagtaata
     2761 ctcaaccgct ttcgttgctt ttccgatgtg gcgttcaccc attacattat tcaggataac
     2821 tccctcgata cgaatgtccg gatcaaatgc ggcaaaccct ttcaccagtg cagcagcact
     2881 gcgtgtaata ctcctggcat tgatcacaag aatcaccggc agatcaaaca tttttgcaac
     2941 cgatgccgta ctgccgacat cagtcagtgc atccacccct tcatacagac cgcgtactcc
     3001 ttctacgatt ccgtactccg ctcccaccga accataactg aacagaccag atgtctcttc
     3061 tggagtttgc acaaatgtat ccagattata ggaaggacga ccggttaccc ctgcaagata
     3121 cgacgtatcg atataatcca ttgcgacttt gtacgtctgt actggagagt cctgagcaag
     3181 cagacctgca attgcaagtg ttatacttgt cttccccgca ccggaacgat cacctgaaat
     3241 cagcagagac ctcatgcaac actccggagc acagccccga actcactttc cacaatctca
     3301 tggacaccaa gcgtttttgg atgcagatcg atctcaacca tcacatacgc atgtccctgc
     3361 tctttcagag gggcaacctg tcgcggccca tttgtaacag aaaacactgt tattccctga
     3421 agtgcctcag gaatgacggc atgcggcaca cccaccagca gaacaaaatc agggtgagtc
     3481 tctgcaataa tttctgcaat cgcagaaccg ttctccccat actcatccag agctccgcag
     3541 aggacaggat ccaccccgcg ctcacgcatc tccgcaagaa ttgctgcggc atctccccgg
     3601 actttcggaa gtccccgtcc ctcaaggttt gccacatatg tgatatctgc atcaggaact
     3661 gtatcatgca gcgcaagaag ttcatccgca aacatgtacg cggtctcttt ctttgcattc
     3721 ataatagcaa gaccccgttt accggatctg gcaagtgaaa gaagttcagt tgcggctacg
     3781 tgtttcagat caccgcgtga cggtgaaatg taggtcttac aggcggcacc tcgtgcccgc
     3841 tctgttgcat ttgcggctgc aagtccacgc cgctgccgct caagttcttc agaac
//
LOCUS       DALUAJ010000148         1732 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_26765,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000148 DALUAJ010000000
VERSION     DALUAJ010000148.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26765"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            164..1378
                     /locus_tag="O0X37_03170"
     CDS             164..1378
                     /locus_tag="O0X37_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK61960.1"
                     /translation="MEEMSANKKIRDIICGHFLIDIYTPVLALILPLLIAQMNLSYFL
                     AGLIVTVFNVTSSVSQPFVGLYGDKTGWRASVPLCLLIGSVGISLTALSGNYLLLLFL
                     AAGAAVGHALFHPAAMDIMYRLSPPAKRGFYNSIFTTSGSIGYAIGPVLAGILIALGG
                     LPAVAWLIIPGIVGATWMYRNSRRFRHENPAPEPVRKPVQTVSEKPVQKKKYWWVPAG
                     LVVTICSLRAWAYLGIITYLPTFLVLGHHGLDTFTASLVVTIMLFFGVAGQVVGGYMS
                     DRFGRKEMLILGLAGAIPFFALIFSTNELLMYLGIMVYAFFASSCYVMSVTMTQDLLP
                     GNVGFASGLTLGFSMGVGGVGAAVIGWAADMMGSLAPALYLLIIPTILCPVLALFIKY
                     PLKSLARKKPSA"
     gene            complement(1404..>1732)
                     /locus_tag="O0X37_03175"
     CDS             complement(1404..>1732)
                     /locus_tag="O0X37_03175"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HJK61961.1"
                     /translation="AGADFLCMVSPSEHLALPDTRDIIEGTRVAKVAAHVGDLVRRRD
                     AELPRQAAMAEARRTLDWEAQYALALFGDHARAVHDRDGVCETCSMCGDLCAIKIVEK
                     ALEKQI"
BASE COUNT          371 a          452 c          461 g          448 t
ORIGIN      
        1 cacaacggta tttgtccatt ttgattttcc ttccttgtaa taaggcacat tactattggg
       61 atcgataatt tatattttgt tgggatgtga catggggggt ggcatttcct gctgcatcac
      121 cgaacttcct ttataaggga tgataccaaa acgtagtgat attatggagg agatgagtgc
      181 aaacaaaaag attcgcgaca ttatctgcgg tcattttctg attgacattt acacgccggt
      241 actagcgctg attcttccgc tgctgattgc acagatgaat ctttcgtact tccttgcggg
      301 actgatcgtg accgtattca atgtgacctc ctcggtatca cagccgttcg ttggtctgta
      361 cggggacaaa accggatggc gggcaagcgt gccgctgtgt cttttgatcg gaagtgtggg
      421 tatcagtctc acagcacttt caggaaacta tctgctgctg ctgttccttg cggcaggggc
      481 ggcagtcggt catgcgctgt tccacccggc ggcaatggac atcatgtacc gcctgagtcc
      541 gccggcaaaa cgcgggttct acaactcaat ctttacgacg agcggaagta tcgggtacgc
      601 gatcggcccg gtgcttgcag gtatccttat cgcactcgga ggacttcccg cggttgcctg
      661 gctgataatt ccgggaattg tcggagcaac gtggatgtat aggaacagcc ggagattccg
      721 ccacgaaaac cccgcaccgg aaccggtacg aaagccggtc cagacggttt cggaaaaacc
      781 ggtacaaaag aagaagtact ggtgggttcc ggcaggactg gtggtcacca tctgctcgct
      841 ccgggcatgg gcataccttg gtatcattac ctatcttccg accttccttg tccttggtca
      901 ccatggtctt gacaccttta ctgcatcact ggtggtaaca ataatgctgt tcttcggtgt
      961 cgccggtcag gttgtcggcg gatacatgtc ggatcggttt ggcagaaaag agatgctgat
     1021 ccttggtctt gctggtgcaa ttccgttctt tgcgctgatc ttttccacaa acgaactgct
     1081 gatgtatctg ggtatcatgg tgtacgcatt ctttgcgtcc tcctgttatg tgatgtcagt
     1141 gaccatgacg caggatctgc ttcccggaaa tgtcggattc gcctcgggac tgacactcgg
     1201 attttcgatg ggagtcggcg gggttggcgc agcggtcatc ggatgggcag ctgatatgat
     1261 gggatcgctt gctccggcac tgtatctgct gatcattccg acgatcctct gcccggtact
     1321 ggcactcttt atcaaatacc cgctgaagag tcttgccaga aagaaacctt ccgcataact
     1381 ttttttggaa aagaggagac agattagatc tgtttctcaa gtgctttttc cacgatctta
     1441 attgcacaca gatcgccgca catcgaacat gtctcgcaga caccgtcacg gtcatgcacc
     1501 gcacgggcgt gatccccgaa gagagcaagt gcatactgtg cctcccagtc cagagtccgg
     1561 cgggcttcgg ccatcgccgc ctgccggggc agttctgcat cgcgtctgcg caccagatca
     1621 ccgacatgcg ccgcaacctt tgcaacccgt gttccctcaa taatatcccg agtatcagga
     1681 agcgccagat gctccgaggg tgacaccata cacaggaaat ccgcacctgc cg
//
LOCUS       DALUAJ010000149         2154 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_26903,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000149 DALUAJ010000000
VERSION     DALUAJ010000149.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26903"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(279..351)
                     /locus_tag="O0X37_03180"
     tRNA            complement(279..351)
                     /locus_tag="O0X37_03180"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(315..317),aa:Glu,seq:ttc)
     gene            complement(433..1296)
                     /gene="ilvE"
                     /locus_tag="O0X37_03185"
     CDS             complement(433..1296)
                     /gene="ilvE"
                     /locus_tag="O0X37_03185"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HJK61962.1"
                     /translation="MLVYVNGKFVPEEQATVSIFDHGFLYGDGVFEGIRAYNGRVFRL
                     MEHVDRLFDSAKAIDLIPPVTKDEMAEIIKETLRRNNLKNAYIRPIISRGIGSMGLDP
                     HHCEKPTVICAASEWGAMYGDLYEKGLTAVTVCVRRNTPDSLPPNIKSLNYLNNILGK
                     IEANYKGGDEAIFLDRTGKLAEGSGDNIYLVKGGKLYTPPTINNLKGITRHVLLELLD
                     KLEIPYEICELGLFDLYTADEVMVTGTAAEICAITKIDGRVIGDGKPGAVYRRLIPAF
                     QEVTQNEGDAY"
     gene            complement(1486..>2154)
                     /locus_tag="O0X37_03190"
     CDS             complement(1486..>2154)
                     /locus_tag="O0X37_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HJK61963.1"
                     /translation="PDSDLIRSAAAKAHVYVSIGVTVRDATSATLYCTNLFFGPDGTL
                     LGKHRKLKPTGTERCIWGEGDGSTLPIIRTPFGVMGSLICWENYMPLARAAMYQKGVS
                     LYLAPTADSRDEWQATMRHIALEGRCFVIGCNQYVTKSMYPPDLYCGGELKDLPENMC
                     PGGSCIYDPFGRCVAGPVFDREEILYADLALDDIVLSRMDFDPSGHYSRPDVFVLKVC
                     EPDR"
BASE COUNT          590 a          519 c          512 g          533 t
ORIGIN      
        1 aacctcgtca ccattgtcgt accagatctc accgttaccc aggtggtaac ttaatttaca
       61 cggttttttg gcatgtgttg cttcgtcaat gagggtcacg tccaagataa gccagccctc
      121 agtatcggag tagtgaacag cttcaatacc gtttgcgagc tctaaaacct ctctggggtt
      181 atcccgtcct cgtacgatta caagatacat actgtgatct tttgtgatcc aacaaagata
      241 aacattcaga aacaggtaag ataaaacaaa aaggttagtg ctgatatcgg gattcgaacc
      301 cgagtcgctg gcgtgaaagg ccagcatgat tggcctctac actatatcag cccaatcact
      361 gctgcatcgc agtattgcct atagagtttg tcgtgaaaaa ataaaaagat tatgatatca
      421 atctgatgag ggttagtatg cgtctccttc gttctgggtg acttcttgga atgcagggat
      481 cagtctgcgg tacactgcgc caggttttcc atcgccaatc actctgccat caattttggt
      541 gattgcacag atttctgcgg ctgttccggt aaccataacc tcgtcagcag tgtacagatc
      601 aaacagacca agttcacaga tttcataggg aatctcaagt ttgtccagaa gttcaagcag
      661 gacatggcgc gtaattccct tgaggttgtt gatggtcggc ggggtgtaga gtttaccgcc
      721 tttgaccaga tagatgttgt caccggagcc ctcagcaagt tttccggtac ggtcaaggaa
      781 gatagcttcg tcaccgcctt tgtagtttgc ctcgatctta ccaaggatgt tgttgagata
      841 gttcagtgat ttgatgttgg gcgggagaga gtcaggagtg ttgcggcgga cacaaacggt
      901 tactgcagtc agaccttttt catacaggtc gccatacatc gcaccccatt cagatgctgc
      961 acagatgacc gtcggttttt cacagtggtg agggtcgagg cccattgatc cgataccacg
     1021 gctgatgata ggacggatgt aagcattttt caggttgttc cggcgcagag tctctttgat
     1081 gatctcggcc atctcatctt tggtaaccgg cggaataaga tcaattgctt ttgctgagtc
     1141 aaagagccgg tccacatgtt ccattaaacg gaacacacgg ccgttgtatg cacgaattcc
     1201 ctcaaacaca ccgtcgccgt agagaaaacc gtgatcaaaa atggatacag tcgcctgctc
     1261 ttcagggaca aattttccat tgacatacac taacatgatt gagttgtttg tcgggattgc
     1321 tatatctaat gtgcggctca ctatcggaag agataggaaa aacgcataga aacacacaat
     1381 taagacccgt acattactcc caagtcccga gaactcgcgg ctgaaaaata tgagagtcag
     1441 tgcccatact ccattcactg gaaaatacag ccaggtacgt gaaatctatc gatcaggttc
     1501 acagactttc agcacaaaaa catcaggacg gctgtaatgt ccagagggat caaaatccat
     1561 acgggataaa acgatgtcat cgagcgcaag atctgcatac agaatttctt cacggtcaaa
     1621 aacaggacct gcaacgcagc ggccgaacgg atcgtagata cagctacctc cgggacacat
     1681 gttctccggc agatccttca attcacctcc acagtacaga tcaggaggat acatcgattt
     1741 ggtcacgtac tgattgcaac caatcacaaa gcatctgcct tccaaggcta tgtggcgcat
     1801 ggttgcctgc cattcatcgc gcgaatcagc agtaggtgca agataaagag aaactccttt
     1861 ctggtacatc gcagcacgcg caagcggcat gtaattttcc cagcagataa gagaacccat
     1921 cacgccaaac ggcgtgcgaa tgatcggaag agtgcttccg tccccttccc cccagatgca
     1981 ccgttcagtt ccggttggtt tcagcttccg atgtttcccc aaaagagtac catccggacc
     2041 aaaaaagaga tttgtacagt agagggttgc agaagtagca tcgcgtacag tcacaccgat
     2101 ggagacatag acatgagctt tggcagcggc actgcggata agatccgaat cagg
//
LOCUS       DALUAJ010000150         2540 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_27128,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000150 DALUAJ010000000
VERSION     DALUAJ010000150.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27128"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            39..893
                     /locus_tag="O0X37_03195"
     CDS             39..893
                     /locus_tag="O0X37_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HJK61964.1"
                     /translation="MGGVITEADGEHCTVRLRMPAGIATADQLIAAAELAKKYGTDEL
                     HFTTRQTLEIPHIPADQLPSFYEELIAAGNDVGAERGEVVNITACPGTHRCKFANIDS
                     VGMAKKIDAKYFGKDMPGKVRIAISACPNACTSETLNEIGITGICTPIRQPGYCTGCG
                     TCVQYCQEEALTVREGKIVMDDDACLDCGTCVRSCIYGILESQPQSYRITLGGRRGRH
                     PKVGMHLITVNTEDAAVQVIDSIIDWIYRYARFDTPIVQQIGQELDMEDLKKNLQKNI
                     PAEQIVVL"
     gene            complement(905..1462)
                     /locus_tag="O0X37_03200"
     CDS             complement(905..1462)
                     /locus_tag="O0X37_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61965.1"
                     /translation="MTDNEKPVFFSTHLHDDLDHAFDDLRRIHDEITAVIRNMEDAAS
                     KCSDPEREKELRFYADAALGPTDDLEKWTTTYEDAVNEKLKGNHLVYERDTYQTLVRI
                     LQWDRVDVRQLARWIRELKELTSHIGLDVPYLLHVKQIPTEPIPEDVEKYPVFVLDRQ
                     GYCLCGMDLAEIRYIDEVRDLMAEK"
     gene            complement(1497..2117)
                     /locus_tag="O0X37_03205"
                     /pseudo
     CDS             complement(1497..2117)
                     /locus_tag="O0X37_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448962.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
     gene            complement(2288..>2540)
                     /locus_tag="O0X37_03210"
     CDS             complement(2288..>2540)
                     /locus_tag="O0X37_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HJK61966.1"
                     /translation="AEGGTIIIKGKAHRRVGGQMVKGEIYVFHGCDVMMPGFKKVEER
                     EIEVDGETHMFNVFIGDLGERHPKSKGEVVYGHIYTLKE"
BASE COUNT          692 a          701 c          550 g          597 t
ORIGIN      
        1 aatataaata gatgaaatcc gactccaaaa tatacgaaat gggaggagta attaccgaag
       61 cagacgggga acactgtact gtccggttac gaatgcctgc ggggatcgca acagctgatc
      121 agcttattgc cgcagcggag ctcgccaaaa aatacggaac agacgaactt cacttcacca
      181 cccggcagac actcgagatc ccgcatatcc ctgcggacca gctcccatca ttttacgagg
      241 agctcattgc cgcaggaaat gatgttggtg ccgaacgcgg ggaagttgta aacatcactg
      301 cctgccccgg aacccaccgc tgtaaatttg ccaacatcga ttcggtcggc atggcaaaga
      361 aaattgatgc caaatacttc ggcaaggaca tgcccggaaa agtccgtatt gccatatcag
      421 catgcccgaa tgcctgtact agtgaaaccc tgaatgaaat aggcatcacc ggtatctgta
      481 caccaatccg ccagccaggc tactgtacag ggtgtgggac atgtgtacag tactgccagg
      541 aagaggcact aacagttcgc gaagggaaaa tcgtcatgga cgatgacgca tgtcttgact
      601 gcggaacctg cgtccgctcc tgtatctacg gaattcttga gtcacaaccc cagtcctacc
      661 gcattaccct tggcggcaga cgcggacgtc accccaaagt aggcatgcat cttatcaccg
      721 tcaacactga agatgccgca gtccaggtga tcgatagtat cattgactgg atctatcggt
      781 acgcaagatt tgataccccc atcgttcaac agatcggaca ggaactcgat atggaagatc
      841 tcaaaaagaa tctgcagaag aacatccccg cagaacagat tgtagtgctg taaaaacaaa
      901 aaaattattt ttctgccatc agatcgcgga cttcatcgat gtagcgaatc tcggcaagat
      961 ccattccgca aagacagtaa ccctgacggt ccagcacaaa cacaggatat ttctctacat
     1021 cttcaggaat cggctccgtt ggaatctgtt tcacatgcag aagatacggg acatcaaggc
     1081 cgatgtgtga tgtaagctct tttaattccc gaatccatcg tgccagctga cgaacatcaa
     1141 cacgatccca ttgaagaatc cgcaccagcg tctgatacgt atcccgctca tacaccagat
     1201 gatttccttt cagcttctca ttcactgcat cctcataggt cgtggtccac ttctccagat
     1261 cgtcagtggg gccaagggct gcatccgcat agaatctcaa ctccttttcc cgttcaggat
     1321 cagaacattt ggatgccgca tcctccatgt tgcggataac agcagtgatc tcatcatgaa
     1381 ttctccgcag atcatcaaac gcatggtcaa gatcatcatg gagatgggta ctaaagaaaa
     1441 ccggcttttc attatcagtc ataaaaatct ctcaaaaaat gtttgatctg agaaagttat
     1501 ttcttcagat agaggttcac atagaccgtc tggccttttg atgtcgggtg gcgctcacca
     1561 agatcgccga tgtagtgggc aaagacagcc ttttctccgt caacctcggc ctcaacatcg
     1621 tcaacgtatc tgtatccggg catcatatac tcaatcttgc cgaaaacata gacgtcaccc
     1681 tttactgcct gaccgccgac acgcccctct acatttcctt tgatgatgat ggttccaccc
     1741 tcgccgtggg tcagaacgtg aataaacaca tcgccctcga tgatgatggt tccaccgcgc
     1801 ataaaggtac ctacatcgtt acctgcattg ccggtcacgc ggaagacacc accggtcata
     1861 ccgcgccagt cgccgcggca tgcacagccg acgtggttct gcgcactgcc gtttaccgta
     1921 agttctccac cagccattgc aataccagtg aaggagtcaa cgttaccgtt tacggtaatc
     1981 ttgccgccct tcatccagcc gccggtgtac atatcggcgt tgctgttgat cacaatctca
     2041 ccagcagtca tctgctggcc gatacgcttt acacgagtcc agtcgccgtt caagacgatc
     2101 ttggtctcag ctgcagtacc agcccaaact gactgtgagg aaacatccga actattacta
     2161 ccacatagaa tggggcagag ttagaaagtg tggcatctcc acatacagat ctctgtccta
     2221 ttcttctctg cacttgatgc aaaaaatctg tttttaaaac aagaatacaa aaactgaaaa
     2281 aggagagtta ctccttcaga gtgtagatgt ggccgtaaac aacctcacct ttgctcttcg
     2341 ggtggcgttc gccaagatcg ccgatgaaca cattaaacat gtgggtttca ccgtcaacct
     2401 caatctctct ctcctcaacc ttcttgaaac caggcatcat cacatcacag ccgtggaaga
     2461 cataaatctc acctttcacc atctgaccgc caacacggcg atgagctttg cccttaatga
     2521 tgattgttcc accctctgcg
//
LOCUS       DALUAJ010000151         1523 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_27161,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000151 DALUAJ010000000
VERSION     DALUAJ010000151.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27161"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(128..1354)
                     /locus_tag="O0X37_03215"
     CDS             complement(128..1354)
                     /locus_tag="O0X37_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HJK61967.1"
                     /translation="MTHTGEQKIQWAQQYMPVLNAIRERFEKEKPLAGHRIGMALHVE
                     AKTACLVRTLAAGGAEVYITGCNPLSTQDDVAEALRQGGVACYAHRGCSTEEYYEAID
                     KVLDAHPTLTIDDGMDLINRVHLDRRDVLPEIIGACEETTTGIHRLRAMEADGKLEFP
                     VIAVNDTPMKHYFDNVHGTGESALASIMLTTNVLLAGRHVVVAGYGYCGRGVATKARA
                     LGARVIVTEIDPRRALQAHFDGFDVMSMNEAAKIGDLFITTTGNCSIITGRHFPLMKS
                     GAILSNAGHFNNEIEIPWLAEHATAVEHRDGIDTYVIDGKKIHLLAEGRLVNLATPKG
                     MGHPVEVMDLSFAVQALSVEYVAKHGRELAAGVHDVPTEIDEYIARLKLAAVGATIDE
                     LTDAQKEYMCSWNHGT"
     gene            1426..>1523
                     /locus_tag="O0X37_03220"
     CDS             1426..>1523
                     /locus_tag="O0X37_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HJK61968.1"
                     /translation="MGLTRRIIPCLDLKDGRVVKGVNFEGLRDAGDP"
BASE COUNT          325 a          414 c          382 g          402 t
ORIGIN      
        1 gatgcttacc tgatgacacc aaacgtgttt gcctatgcgg gtcatgcatt tctgatcttt
       61 gcagatgagg ttgttggccg tcggctcatc tggaaaaaaa gtgaataaaa aaatattttt
      121 tattttgtta tgtgccgtgg ttccagctgc acatgtactc tttctgcgca tctgttaact
      181 catcaatcgt cgcacccaca gctgcaagtt tcaggcgtgc gatgtactca tcgatctcgg
      241 tcggcacatc atgcactccg gctgcaagtt cgcgtccgtg ttttgctaca tactcaacag
      301 atagtgcctg taccgcaaag ctgagatcca taacctcaac cggatgaccc attcccttcg
      361 gggttgcaag gttgaccagt cttccttccg caagcagatg gatcttcttt ccgtcgatca
      421 cgtatgtatc aataccgtcg cggtgctcaa ctgccgtcgc atgttctgca agccacggaa
      481 tctcgatctc gttgttgaag tgtcctgcat tggaaagaat cgcaccagac ttcatcagcg
      541 ggaagtgacg gccggtgatg atactgcagt tacctgtcgt tgtgatgaac agatctccga
      601 tctttgctgc ttcgttcata ctcataacat caaagccgtc aaagtgtgcc tgcagtgccc
      661 ggcgcggatc gatctcggtg acaatcacgc gtgcaccgag tgcacgtgcc ttggttgcaa
      721 ccccgcgacc gcagtacccg tatccggcaa ccactacatg gcgtccggca agcagaacgt
      781 tcgttgtcag catgatcgag gcaagagcgc tctcgccggt tccatgcaca ttgtcaaagt
      841 aatgcttcat cggggtgtcg ttcaccgcga taaccgggaa ctccagcttc ccgtcagcct
      901 ccattgcgcg cagacggtga ataccggttg tggtctcctc gcatgcgccg atgatctccg
      961 gcagcacatc gcggcggtcg agatgtaccc ggttgatcaa atccattccg tcgtcgatgg
     1021 ttaaggtcgg atgtgcatca aggaccttat cgattgcctc atagtactcc tcggtactgc
     1081 atccacggtg cgcatagcag gcaactccgc cctgacgaag agcttctgcc acgtcgtcct
     1141 gtgtggaaag cgggttacat ccggtgatgt acacctccgc tcctcctgcc gcaagtgttc
     1201 ttaccagaca tgcggtcttt gcctctacat ggagagccat acctatgcgg tgtcctgcaa
     1261 gcggtttttc cttttcgaag cgttcacgaa tggcgttaag aacaggcata tactgctgtg
     1321 cccactgtat tttctgttcg ccggtatgcg tcatatccag aaatgttggt tgtgcaagcc
     1381 tataatacgc aatgataatt tttcctgccc gctaactata ctggcatggg acttacacgg
     1441 cggattattc cctgtcttga tctcaaagac ggaagagttg tgaaaggcgt gaactttgag
     1501 ggtctgcgtg atgcgggcga tcc
//
LOCUS       DALUAJ010000152         2478 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_27452,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000152 DALUAJ010000000
VERSION     DALUAJ010000152.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27452"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="O0X37_03225"
     CDS             <1..246
                     /locus_tag="O0X37_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HJK61969.1"
                     /translation="TIVPSRSASLLMRINQRSLAKAVLVFLIVVSFVFCGPFGLMILA
                     AATLVGLVPGLLDVPRIFCMGAIMVPVMLFTLDVLRL"
     gene            complement(249..1241)
                     /locus_tag="O0X37_03230"
     CDS             complement(249..1241)
                     /locus_tag="O0X37_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HJK61970.1"
                     /translation="MTPATNNFREFETGNEFEPKVPIRFIGSATPVSDFLQLYDNRKT
                     RENYRLAISAYLRCFNPGTFKRPHEIDPHAVTYLRELMGGRNYHHDLRMAGNIFSRSY
                     APMTANLYLKATCVWLEDSGFPLSRRERSRIFVSLPPARAATQEIELKRRVFQEMYFE
                     LPEHIGVLLLTLTGSGMRLGEATKLLKTDLDETQSRTAVNIRAETTKTKTARTTYLTQ
                     EASEALDDYFASRKDNDPRVFPISPLTAQYHFRKTADLLGYGNPDPATKCRQLHWHMT
                     RKWFISRFSLAASKDIAELLAGHEGYLAASYQRYTRKQILKQFKKAEREISILR"
     gene            1419..2411
                     /locus_tag="O0X37_03235"
     CDS             1419..2411
                     /locus_tag="O0X37_03235"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HJK61971.1"
                     /translation="MLNKYKGCLLGAVLGDALGMPGETTVSRFIGVTLGFKRAYKGHP
                     NHDLLPGQYTDDSQLILIASRLLAEASWDPESYAKELLRTYNLNKFRYPDGTIYAACK
                     RMKTTDDLVGSGVYSDSAGCVSLAVPFALAYKDRKEMAPRLLEACNITHTHPAVHAAT
                     LGFALLLNTLIETGSTDAGYKALFTAAENMDPDLAARLKNAVRIEGTGMQIDDALAAI
                     GNSSSVYHTLPLAVFLCKRYDVPDELLAYASACGGNADTLTLLCGAFAGARYGISALP
                     QDLVAQLERRSEFELLAEKLLNPQKPEPPAPEKTVTEKSPEEPESIYDSGWEPE"
BASE COUNT          561 a          633 c          621 g          663 t
ORIGIN      
        1 acgattgttc catcacgatc cgcctctctg ctgatgagga taaatcagcg gtcgcttgca
       61 aaagcagtgc ttgtgttctt gattgtggtg tcgtttgtgt tctgcggacc gttcggcctg
      121 atgatcctgg cagccgctac gctggtagga ctggttcctg ggctattgga tgtacccagg
      181 attttttgca tgggggcaat tatggttccg gtaatgttgt ttacgctgga tgtgctccgg
      241 ctctgagcct accggagaat actgatctcg cgttctgctt ttttgaactg tttgagaatc
      301 tgttttcggg tatagcgctg atacgatgcg gcaagatatc cttcatgacc tgcaagaagt
      361 tcggcaatat ctttgcttgc cgcaagactg aaccgggaga taaaccattt gcgggtcatg
      421 tgccagtgga gctgacgaca ttttgttgca ggatctgggt tcccgtatcc gaggagatcg
      481 gcagtttttc tgaaatgata ctgggcagtg agcggtgaga tgggaaacac ccgcggatca
      541 ttgtctttgc ggctcgcaaa gtagtcgtcg agggcctcgg atgcttcctg ggtgagatag
      601 gtggttcgtg ctgtttttgt tttggtagtt tctgcacgga tgttgacggc ggtccgtgac
      661 tgtgtttcat caagatcggt tttgagaagt ttggtggctt ctccaagccg catcccggaa
      721 ccggtcaggg taagaaggag aacaccaatg tgttcgggca gttcaaagta catttcctga
      781 aagactctgc gtttcagttc tatctcctgt gttgccgccc tggccggggg gagactgaca
      841 aaaattcgtg atcgttcccg tctgctgaga ggaaaaccac tgtcctcaag ccatacacag
      901 gttgctttga ggtagaggtt tgccgtcatg ggagcatagc tccgggaaaa gatgttccct
      961 gccattctga gatcgtgatg atagtttctg cctcccatga gttcccggag ataggtaact
     1021 gcatgcggat cgatctcatg gggtcgtttg aaggtcccgg gattaaaaca cctgagatag
     1081 gcactgattg caagccggta gttttccctg gtctttctgt tgtcataaag ctgcagaaaa
     1141 tcactgacgg gagttgctga accgataaag cgaataggta cttttggttc aaactcgttt
     1201 cctgtttcga attctctgaa attgttggtg gctggtgtca ttgttgtttt cactcatccc
     1261 cgaattgacc gtttgttgtg ctggggttta gttagtatgt acttcccggc ggccatgttc
     1321 tcaacatcaa tttcacacag actgaagtct ctgctgcaca aaaagatcaa aaaaacacac
     1381 cataacaatt aacctccctc cctcccataa cataatacat gctgaacaaa tacaagggat
     1441 gcctgctcgg cgcagtactt ggcgatgccc ttggcatgcc cggcgaaacc accgtaagcc
     1501 ggttcatcgg agtcacactc ggatttaaac gagcatacaa aggccacccg aaccatgatc
     1561 ttctccccgg tcagtacacc gatgactccc agctcattct cattgcatcc cggctgcttg
     1621 ccgaagctag ctgggatccc gaatcatacg caaaagaact tctccgtacc tataatctca
     1681 acaagttccg ttacccggac ggaactatct acgccgcatg caaacgcatg aaaacgaccg
     1741 acgatcttgt tggttccggg gtatactccg acagtgccgg atgcgtctcc cttgcggttc
     1801 cctttgctct tgcctacaag gatcgtaaag agatggctcc aagactgctt gaggcatgta
     1861 atattaccca tacccacccc gctgtccatg cagcaaccct tggatttgct cttcttctga
     1921 atacgctcat tgaaaccgga agtaccgatg caggctataa ggccctcttt actgcggcag
     1981 agaacatgga tccggatctg gctgcccggc tcaagaacgc tgttcgcatt gaaggaaccg
     2041 gtatgcagat cgatgatgcc cttgccgcaa tcggcaactc ctcttcggtg tatcatacgc
     2101 tcccgcttgc ggtcttcctc tgcaaacgct acgatgttcc ggatgaactc cttgcctatg
     2161 cttcggcctg tggtggtaat gccgacacgc ttacccttct ttgcggtgca ttcgccggtg
     2221 cccgctatgg aatctcagca cttccacagg atcttgttgc ccagcttgaa cgaaggagtg
     2281 agtttgaact cctcgcagaa aaacttctga acccccagaa gcccgaacca ccggcacctg
     2341 aaaaaaccgt cacggagaaa tcaccggaag aaccggaaag tatctacgac tccggctggg
     2401 aaccggaata accctcttct tttttcacat tcataacaga atttaatctc tttcacaacc
     2461 catactgtag tcatggac
//
LOCUS       DALUAJ010000153         6525 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_27486,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000153 DALUAJ010000000
VERSION     DALUAJ010000153.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27486"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="O0X37_03240"
     CDS             <1..1183
                     /locus_tag="O0X37_03240"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HJK61972.1"
                     /translation="MKTERKAASILSRTPKGKIPLDITVGVNENGHLAVIGNRAAAVS
                     ESPMQDAQTRPVTREALEAQLRKTGGTPFVIRSIVMNYDGTKFLRMGVINELRRRFLA
                     ACSDALTVPPVRTHRPFSEKGTATPEPMKPIIQVYTDSIVAAKAASTAGAGQVVYEGT
                     DEIYDHAIIYKLPRILHEEELAKTLARLPASAAGVMVDGVGVAEEIKGIPKYGGSGLN
                     ITNARSAVVYGKTCRQVCLSPELSGKQIKVLMNHLAAYAHPPKIEVIVQGSLEVMITR
                     NCIPATALGCRSCNQPWALKDATGRIFRVRTDANCCSHILNAAEICLIEHVQTLAATG
                     VSVLSIDARGRSPEYVRRMIALYTAALEGENAKELKEQVRDLASGGVTAAHYLRGVEK
                     E"
     gene            1423..2826
                     /locus_tag="O0X37_03245"
     CDS             1423..2826
                     /locus_tag="O0X37_03245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK61973.1"
                     /translation="MEWDFMNNNQHYTGSKLLVILLAATLASFLTPFYTSMVNIAIPA
                     IGESFTVPAETLAMLSTVYLISSVIFLVPAARIADIFGLRKVFVGGLCISAFASFVAP
                     FSISIGMLLACQAVAGIGFACVISNAIALLSIVYPPSRRGAAIGIAVAGVYLGLTAGP
                     LIGGALTSVFGWSSIYLFVLLLCLITAIMVRLSISTELFTTPDQPFDLRGSVLYGVVV
                     LFLMLGLINLPDPFAVAGLIFGIILLVLFIRFETKIQYPVFPVRLFAENQVFARANIT
                     TMINYGATFAVSFFMSLYLQVIGTLTPMEAGVVMIAMPVIQMIFSPIAGRLSDKIGGR
                     VLMVAGLFVTGIGMALFGFIGTEYNQTAIILNLTVVGFGIALFGAPNTNLVLGSVDFR
                     DNGSANSILATMRQMGMVISMAVATCCVMLFTSTEAELGINASGLVSAMHLTFALGAI
                     LSFLGAFLGWTAVPKKE"
     gene            2859..4274
                     /locus_tag="O0X37_03250"
     CDS             2859..4274
                     /locus_tag="O0X37_03250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK61974.1"
                     /translation="MTTGYQYTIREMQMVLVAAALASFLSPFLGSMVNVAIPAIGEAY
                     VATAESLAMLSTAYLISSVMFMVPAARIADILGRKKIFLAGIFLLAVSSILAPFSPSI
                     EILILCRIIEGVGVAAITSNSIALLSAVYPPQKRGAVIGYAVSAVFLGLSAGPVLGGV
                     LTQILGWESIFYFVVVLAVFTFAAIWFSISHDIRENEGEPYDYIGTCVYMLLILTLVL
                     GLIDLPKPWAVGLLAVGCVVLLPLFVFVEKRTQYPVFALRLFSGNRVFTRGNLATVIN
                     FGATYAISFFLSLYLQVAGMLTPMEAGVVLVAMPFTQMIFSPLAGRISDKIDPRYLTT
                     AGMLLMAGGLTVLLQLGAVLDLPMLLATLVLMGFAVALFASPNNNLILGSVQRREYGS
                     ANSIIGTMRQMGMVLSMGLATCFISIFLGTTTEMAENLDAFLSAMHLSFACGVVFCLI
                     AAVLSFSSKPLLTADIQRNTR"
     gene            complement(4371..4442)
                     /locus_tag="O0X37_03255"
     tRNA            complement(4371..4442)
                     /locus_tag="O0X37_03255"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4407..4409),aa:Arg,seq:gcg)
     gene            complement(4517..5920)
                     /locus_tag="O0X37_03260"
     CDS             complement(4517..5920)
                     /locus_tag="O0X37_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HJK61975.1"
                     /translation="MFLDGTGHDSLSGSSIPVYNPATGEVIDTVPAGNADDVAAAVTA
                     AEIAQKKWEQVSPADKSRILIAAGALIRSEADALATLLTTEQGKPFREAKDEILGTAH
                     VFEFYASMTGTLQGDAAVLPKYGYMNVVRKPLGVCGAIVPWNMPAIIFGWKAGAALAC
                     GNAVIAKPSETAPLTVLRLAELLVKAGVPGGAMNVVTGSGTVAGDAIVRNTGIRHVSF
                     TGSVATGRSVALAAAPTLKKLTLELGGNDAFIVTADADIDAAVAGAVRNRFYNCGQVC
                     TSAKRILVDAVCADEFVRKAKDAIEKLVVGSGLEKVNMGPLNNPSQRDAVAAAVDRIV
                     SEDCGKIICGGKKKEGAGNFYAPTLLADVSPDAVSEEIFGPVMPVIPFTTLDEAVEIA
                     NGTPYGLGASVWTKNLKTAYTVADQIRSGVVWVNRHLILPPEIPFGGTGNSGYGRENG
                     REFIYEYTEPKSILLGL"
     gene            complement(5997..>6525)
                     /locus_tag="O0X37_03265"
     CDS             complement(5997..>6525)
                     /locus_tag="O0X37_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK61976.1"
                     /translation="MKLQRNIAIILAAAFVLLPALAGAAAAADSSTETLQRVVLFQSP
                     EMSNHYGYITVDSVDVELNGVNAIYTVNYSIDPWIAFLVFLLGKQDLKNRLLKIINPP
                     APSNGQTQEIVFQYVDNDQAIISVSNAAMSYGDNTYWYPEQEFAITIPQITFTASFTK
                     TYNNTRVIERGFGYY"
BASE COUNT         1596 a         1663 c         1536 g         1730 t
ORIGIN      
        1 gatgaagacc gaacgaaaag ccgcatccat cctctcacgg acccccaaag gaaaaattcc
       61 gcttgatatt accgttggcg taaacgaaaa cggtcatctc gcagttatcg ggaatagagc
      121 agctgctgtc agtgagagtc cgatgcagga cgcacagacc cgacctgtta ccagagaggc
      181 acttgaggca cagcttcgta aaacaggcgg caccccgttt gtgatccgca gtattgtaat
      241 gaactacgac ggaaccaaat tcctgcggat gggggtcatc aatgagctcc ggcgcaggtt
      301 ccttgctgca tgcagtgatg cgttaaccgt cccgccggta cgaacacacc ggccgttcag
      361 tgaaaagggt actgcgaccc cagagccgat gaaaccgatc attcaggtct atactgactc
      421 catcgttgca gcaaaagcgg caagtacagc cggagccgga caggttgtct atgaaggaac
      481 cgacgagatt tacgatcatg caatcatcta caaactcccg cggattcttc acgaggagga
      541 gcttgccaaa acacttgcca gactcccggc atctgctgcg ggagtcatgg tggacggagt
      601 gggggttgca gaagagatca aaggcattcc aaagtacggc ggttctggtc tcaacatcac
      661 caatgccaga tctgctgtag tgtacggcaa aacatgccgg caggtctgtc tttcgccgga
      721 gctttccgga aagcagatca aagtactcat gaaccatctt gcagcatacg ctcatccgcc
      781 aaagattgag gtcatcgtgc agggaagtct tgaggttatg ataacccgca actgtattcc
      841 tgcaactgca ctcggctgcc ggagctgtaa ccagccgtgg gcacttaagg atgcaaccgg
      901 cagaatattc cgggttagaa cagatgccaa ctgctgtagc catatcctca atgcagcaga
      961 gatctgtctg atcgaacacg ttcagaccct tgccgcaact ggcgtgtccg tactttccat
     1021 tgatgcccgc ggcagatcgc ctgagtatgt ccgcagaatg atagccctct ataccgcagc
     1081 tcttgaagga gaaaacgcca aagagctcaa ggaacaggta cgtgaccttg cttcaggcgg
     1141 tgtaaccgca gcccactatc tccgcggtgt ggaaaaagag taaaaaaccg atttctggtt
     1201 caaaataacc atctcaacca atattagaga gaacattttt tatagtggta gcaactacac
     1261 aatatatctg ttagtatcat ggactaatat cttattctgt cccattacgt gggtatttcc
     1321 gtggtgctag actctacgct tactaaccgt aagaccaaaa tttgggtctc gaggactttt
     1381 tcacaatgaa cgaaaagttt cagtaacgac ggttaggagt aattggaatg ggattttatg
     1441 aacaacaatc aacattatac gggaagcaag ttgctcgtaa ttcttctcgc tgcgacgctt
     1501 gcatcatttt taactccctt ttacacctca atggtgaata ttgcaatacc tgcaattggt
     1561 gaaagtttta cagtccccgc agaaacgctt gcaatgctct caacagtgta cctaataagt
     1621 tccgtgatct ttcttgtacc tgcagcacgg attgccgata tctttggttt gagaaaagta
     1681 tttgtgggtg gtctctgcat atcagctttt gcttcgtttg ttgcaccatt ctcaatctca
     1741 attggaatgt tgcttgcctg tcaagcagta gcgggtattg gattcgcctg cgttatcagt
     1801 aatgcaattg cactactatc aattgtgtat cccccatcac gacgtggtgc agccattggg
     1861 attgctgttg caggtgtata cctgggatta actgctggtc ctcttattgg aggggcacta
     1921 acatctgtct tcggttggtc aagtatctac ctctttgtat tgctactgtg tcttataact
     1981 gccataatgg ttagactatc gataagtact gaattgttta ccacccccga tcaaccattt
     2041 gatcttcgtg gatcggtcct ttatggcgtg gtagtgttat tcctaatgct tggtttaatc
     2101 aacttaccgg atccatttgc tgtagctggt ctcatttttg gaataattct cctggtactt
     2161 ttcatcaggt ttgagacaaa gatccaatac ccagtgttcc cagtccggct ctttgccgaa
     2221 aatcaggtgt ttgcccgcgc caacataacg acaatgatca actacggtgc aacatttgca
     2281 gtcagtttct tcatgagcct ataccttcag gtgattggta cgctgactcc aatggaagca
     2341 ggtgttgtta tgattgccat gccagttata caaatgatat tctccccaat tgcaggacga
     2401 ctctcggata aaatcggagg tagagtcctc atggttgctg gacttttcgt aacgggtatt
     2461 ggtatggcac tgttcgggtt cattggaact gagtataatc agaccgcaat tattctcaat
     2521 ctcacagtag ttggattcgg tattgcacta ttcggtgctc caaacaccaa tcttgttctt
     2581 ggatcagtag atttccgtga caatggcagt gccaacagca ttctcgcaac aatgaggcag
     2641 atgggaatgg tcatctctat ggctgttgca acctgttgtg tcatgctctt taccagtaca
     2701 gaagctgaac ttggaatcaa tgcttcgggt cttgtctctg caatgcacct gacttttgct
     2761 cttggggcaa tcctgtcttt cctcggtgca ttcctcggct ggactgctgt tccgaaaaaa
     2821 gaataatatt attatcacat caaaccgaca aagaacatat gacaacggga taccagtaca
     2881 caatacgcga aatgcagatg gtactggttg cagccgccct tgcttccttt ttaagtcctt
     2941 tccttggatc catggtaaac gtggcaatac cggccattgg cgaagcctat gttgcaaccg
     3001 ccgagtcgct tgcaatgctt tctacagcat atctcatcag ttcggtgatg ttcatggtgc
     3061 ctgccgcacg aattgccgac atcttaggca ggaaaaaaat atttctggca ggaatttttc
     3121 ttcttgcagt ctcatccatt cttgcgccgt tctcaccatc aattgagata ttaatcctct
     3181 gcagaattat tgagggagtc ggtgttgcag caattacgag caactcgatt gcacttctct
     3241 cagcagttta tcctccgcag aagcggggag cagtcatcgg gtatgcggta tctgcggtgt
     3301 ttctcggcct gtcggcaggt ccggttcttg gcggggttct gacgcagata cttggatggg
     3361 aaagtatctt ctactttgtt gtggtgctgg cggtgtttac atttgctgca atatggttct
     3421 ccatctcgca cgacatccgg gaaaacgagg gagaaccgta cgactacatc ggaacatgtg
     3481 tgtacatgct tctcattctt acgcttgttc ttggtctcat tgaccttccg aaaccctggg
     3541 ccgtaggcct gcttgccgta ggatgtgtgg tgctgcttcc gctgtttgtt ttcgttgaaa
     3601 aacgcactca gtatccggtg tttgcactcc ggctcttttc cggaaaccgc gtgtttaccc
     3661 gcggaaacct tgcaacagtg atcaacttcg gggcaacata tgcgatcagt ttcttcctga
     3721 gtctgtatct gcaggttgca ggaatgctta cgccgatgga ggcaggagtg gtcctggttg
     3781 ccatgccgtt tacccagatg atcttctccc ctctggccgg cagaatctcg gataaaatcg
     3841 atccacgcta cctgaccact gccggtatgc tgctgatggc agggggacta acagttcttc
     3901 tgcagctggg tgctgtgttg gatcttccga tgctccttgc aacccttgta ctgatgggct
     3961 ttgccgtggc actgtttgcc tcaccaaaca acaacctgat tctcgggtca gtgcagcgcc
     4021 gtgagtacgg cagcgcaaac agcattattg gtacgatgcg gcagatgggc atggtacttt
     4081 cgatggggct tgcaacctgt ttcatctcaa tcttccttgg caccaccaca gagatggcag
     4141 aaaatctgga tgcatttctt tccgccatgc atctgtcatt cgcctgcggg gtagtgttct
     4201 gcctgattgc agcagtgctc tccttctctt ccaaacccct cttaacagca gacattcaga
     4261 gaaataccag gtaaaaatcc gcaaagcgga tcaaactcca gtctattttt gcagatgata
     4321 cagacatcac ataacatctg aacgaattgt acaagacaaa aaaacagcta cgtcccggct
     4381 gggacttgaa cccaggtcaa aagctccgca ggcttctagg atatcctcta ccctaccggg
     4441 actaaacgca tctatgtgct ctaatacatt ggatatggat tgatataaac ccaccgtttc
     4501 atatttttca aaaaaatcag aggccgagga gaatgctctt cggttccgtg tattcataga
     4561 tgaactctcg tccgttttct cgtccatatc cggaatttcc ggtacctccg aagggaattt
     4621 ccggcggcag aatcaaatgc cggtttaccc acacaacacc ggatctgatc tgatccgcaa
     4681 cggtgtatgc cgtcttcagg ttctttgtcc atactgacgc gccaaggcca tacggcgtac
     4741 cgtttgcaat ctcgaccgct tcatcaagcg tcgtaaatgg gatcaccggc atgaccggcc
     4801 caaagatctc ctcggacaca gcgtcaggag agacatcagc aagcagagtc ggcgcataaa
     4861 agtttcctgc cccttccttc tttttcccac cgcatatgat ttttccgcag tcttcgctga
     4921 caatccggtc aacagctgct gcaactgcat cacgctgact gggattgttc agcggcccca
     4981 tgttaacctt ttcaagaccg ctgccaacaa caagtttctc gattgcatcc ttggcctttc
     5041 ttacaaactc atcagcacag actgcatcca ccaggattcg ttttgctgag gtacatacct
     5101 gaccgcagtt atagaaccgg tttctcaccg ctcccgcaac agccgcatca atgtctgcat
     5161 ccgctgtcac aatgaacgca tcatttccac cgagttcaag cgtgagcttt ttcagagtag
     5221 gtgctgccgc aagagctaca gaccgtccgg ttgcaaccga accggtaaat gaaacatggc
     5281 ggataccagt attccgtacg atcgcatcgc ccgcaacagt accacttccg gtaacaacgt
     5341 tcatggcacc acccggaaca ccggctttta caagaagttc cgccagacgg agaaccgtca
     5401 ggggcgctgt ttccgacggc tttgcaataa ctgcgtttcc gcaggcaagc gctgcgcccg
     5461 ctttccatcc aaaaataatt gcaggcatgt tccatggcac aatggctccg cagacaccaa
     5521 gcggttttct gacaacgttc atgtatccgt acttcggcaa aacagctgca tctccctgga
     5581 gggttccggt cattgaagca taaaactcaa agacgtgggc tgttcccaga atttcatctt
     5641 ttgcctcgcg aaacggcttt ccctgttcgg tggtcagaag cgttgccaaa gcatccgcct
     5701 cgctgcggat aagtgccccc gctgcgatca gaatacggga tttgtctgcc ggacttacct
     5761 gttcccactt cttctgggca atctccgctg ctgtgaccgc tgctgcgaca tcatccgcat
     5821 tcccagcagg aacggtatca atcacttccc ctgttgccgg attatagaca ggaatcgaac
     5881 ttccggaaag gctgtcatga ccggtcccat caagaaacat ctgcatagat gttgtatttg
     5941 atcgaaaagt gtttagagat gatcatcacg gcagaatttt ccgttcaaaa aaaagattag
     6001 tagtagccga atccgcgctc gattacgcgg gtgttattgt aggttttggt aaacgatgca
     6061 gtgaaggtaa tctgcgggat cgtgatcgca aactcctgtt ccggatacca gtaggtattg
     6121 tctccatagc tcattgcggc atttgagacc gagatgatcg cctgatcatt atccacatac
     6181 tggaacacaa tctcctgcgt ctgaccatta cttggggcag gagggttgat gattttgagc
     6241 agacggttct tcagatcctg ttttccaaga agaaatacca gaaacgcaat ccacggatca
     6301 atagagtagt ttacagtata gatggcattt accccgttca gttctacatc cacggaatca
     6361 acggtaatgt agccatagtg gtttgacatc tccggacttt ggaacaggac cacacgctgc
     6421 agagtctccg tactggagtc ggcagcagct gcagctcctg ccaatgcagg cagaagaaca
     6481 aatgccgccg caagaatgat ggcaatattc cgctgtagct tcatc
//
LOCUS       DALUAJ010000154         1747 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_27547,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000154 DALUAJ010000000
VERSION     DALUAJ010000154.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27547"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            178..1362
                     /locus_tag="O0X37_03270"
     CDS             178..1362
                     /locus_tag="O0X37_03270"
                     /inference="COORDINATES: protein motif:HMM:NF017485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJK61977.1"
                     /translation="MTSPAHLLHSLVLFRNLLRLPVYQKLLALLDDDQSDISRLLDRY
                     AEFVAELYCHTDNLSEYIQKSVYEDDNVYVRLVAEGKTIRPAVAETLQSELRTLEMLS
                     RLTPADLKGDYSGYLPSWQTAKIDLVGGYAERMANIQTTGYGIFAAHHVFIIKNEIIT
                     PLKHPDTQKLSDLYGYEAERRKVIANTKALLAGKPSANVLLYGDAGTGKSSTVKAIAN
                     EFASDGLRLIEINKSQLYLIPSVIEQLAANPLKFILFVDDLTFTETDNEFASLKAILE
                     GSAAARTKNIVVYATSNRRHLMKETFSSREGDDVHRNDTLEETISLSARFGIIITFQK
                     PRADAYLEIVDSLAREYNLSVGKDQIHAGAEKYALRAGGRTPRAAKQYIELLAANVLD
                     TV"
     gene            1605..>1747
                     /locus_tag="O0X37_03275"
     CDS             1605..>1747
                     /locus_tag="O0X37_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HJK61978.1"
                     /translation="MLLTGAEIIAEVLIEQGATTIFGYPGGAVLNIYDALYEYRDKIR
                     HIMT"
BASE COUNT          483 a          462 c          380 g          422 t
ORIGIN      
        1 gtatcactgt atcctcaatg aaattcagcc gattcgctaa cacgtgacct tttttcaggg
       61 cagattcatc aacgataccg tttcctgtag cagaaaaaag aacgaaatcc ctgttttcaa
      121 attccgccaa aacctatttc ctctctcacc tggaaacata accagcacat accagccatg
      181 acatctcccg ctcatcttct ccacagtctt gtcctgttcc gcaacctctt gcgtctgccc
      241 gtttatcaga aactccttgc ccttcttgat gacgaccaaa gtgatatctc acggctcctt
      301 gacaggtatg cagagtttgt tgccgaactc tattgccaca cagacaatct cagtgagtac
      361 atccagaaaa gtgtctatga ggacgacaac gtctatgtca ggcttgttgc tgaaggaaaa
      421 accatccggc cggcagttgc agaaaccctt cagtcagaac ttcggacact ggagatgctc
      481 agccgtctta ctcccgcaga cctgaaagga gattacagcg gataccttcc ttcctggcag
      541 accgcaaaga tcgatcttgt cggcgggtat gcggaacgaa tggcgaacat tcagacaacc
      601 ggctacggaa tttttgcagc ccatcatgtc tttatcatca aaaacgagat catcaccccc
      661 ttaaaacacc ctgacacgca gaaactttcc gacctttatg gatatgaggc tgagcggagg
      721 aaggtcattg caaacacgaa agcccttctt gcgggaaaac cgagtgccaa tgttctcctc
      781 tatggcgacg caggaaccgg aaaaagttct accgtcaaag cgatcgcgaa cgaatttgca
      841 tcagacggtc ttcggcttat tgagatcaac aaatcccagc tgtatctgat accatctgtc
      901 atcgaacagc tcgcagcaaa ccctctcaag ttcatcctct ttgttgatga tctgaccttt
      961 acggaaaccg acaatgagtt tgcttccctg aaagcaattc ttgaaggaag tgccgcggcg
     1021 cgtaccaaaa acatcgttgt ctatgcaacc agcaaccgcc gacacctcat gaaggaaacg
     1081 ttcagcagcc gggaaggaga tgatgttcac cggaatgata cccttgaaga gaccatcagt
     1141 ctctccgcac ggtttggtat tatcatcacc ttccaaaaac ccagagctga tgcgtatctg
     1201 gagattgttg actctcttgc ccgtgaatac aatctgtccg tgggaaaaga tcagattcac
     1261 gcaggtgctg aaaagtacgc ccttcgtgcg ggcggcagaa caccgcgtgc cgcaaaacag
     1321 tacattgaac tccttgcagc aaatgttttg gatactgtat aatccccatc cctgtttttc
     1381 aaacttctgt cagcgcgtcc attgcgcgtc tgtccatctg tattcgaccg gacactctgt
     1441 gtaatcggcg gatagattgt tcagatgatg tggtgtgtca gtgtgtgcca tgccaaaggt
     1501 atacatacct cggacgaact acatataaga gcacgcgcaa ctcaaaaaca gcgtgatagg
     1561 agatgaccaa agagaggaag agatctcttt ggaatgaaaa aaacatgtta ctaactggtg
     1621 cagagatcat cgcagaagtt ctcattgaac agggagccac caccatcttc gggtatcctg
     1681 ggggtgcggt tctcaacatc tacgatgctc tctatgaata ccgtgacaaa atccgtcaca
     1741 tcatgac
//
LOCUS       DALUAJ010000155         2640 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_27932,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000155 DALUAJ010000000
VERSION     DALUAJ010000155.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27932"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="O0X37_03280"
     CDS             <1..225
                     /locus_tag="O0X37_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HJK61979.1"
                     /translation="SEDIAALFGELELSGKNICFLIGGPLGLSPAVLAAVDRTISFSR
                     LTFTHTMIRLLLFEQIYRGFRILRGEPYHK"
     gene            complement(311..988)
                     /locus_tag="O0X37_03285"
     CDS             complement(311..988)
                     /locus_tag="O0X37_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HJK61980.1"
                     /translation="MNMILHKPHVLMISEITADGKLTLKRGASSKILMKHMSHEAEIL
                     LHETRAAYDAIMVGSSTIKIDNSFLTVRMVPGKSPLRVIPSSMADLPLDANVLDTSTV
                     PTVVAVSAAAPKDRVAALQEKGVSVIVAGKEKVDLPLLMDILYREFSVRKLIIEGGPT
                     LNWHMLHHHLVDEIRLIHLPFIVGGNDTPSLVGGMHIDSEDQMIRLELSGTRMVGTNL
                     ITEYRVL"
     gene            1106..1753
                     /locus_tag="O0X37_03290"
     CDS             1106..1753
                     /locus_tag="O0X37_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HJK61981.1"
                     /translation="MNITALRPRLIGCIDPIGNAFVRIGLKPNQITFLSLIFGIACAV
                     CYMQRLFLLGSILLGVSAILDLVDGNVARKTNRKSDFGAVLDWIIDKYVDGIVLLGIG
                     LSGTAVISQFLTVPAWSDTAIVGLAIIGSLMNTFIKPVTYAEIGYTKKEDGKISDPLE
                     GIGFFGRPETIICLILFGLISQIWIAVILIAVCTNLSALQRILYLARRHGSYKEE"
     gene            complement(1767..>2640)
                     /locus_tag="O0X37_03295"
     CDS             complement(1767..>2640)
                     /locus_tag="O0X37_03295"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HJK61982.1"
                     /translation="LAYLHDADMAVREAALLLFMEGEKPACDALVAAVSSENEELQFS
                     AVQALGQFRRPEAIDPLIQAAGAKNPDIREVAILSLGVYSEKRVVPLLLASLKDEPQI
                     RLAALEGLRGRELSPEDIVTVLECLHDTDFPEIRAAAVAVLGPLVPEEFTEDPSPQVR
                     RVGASVVASQKLLARLCGDTDASVRMAAAESLGKQEELQEDVLLPLLTDSVPGVRRAA
                     VTALASSRRPDVVAALIDRLHDPKRGVQAAAATVLGEIGGDEVVVALTEASKSGNVIL
                     RGITKNALNAALKK"
BASE COUNT          642 a          671 c          595 g          732 t
ORIGIN      
        1 agcgaagaca ttgccgcatt gtttggggag ctggagcttt ctggaaaaaa tatctgtttt
       61 cttattggcg ggccgctagg actgtcacct gcggttcttg ccgcggtgga tagaacgatc
      121 tcattttccc gcctgacatt tacacatact atgatccgtc tgcttttatt tgaacagatc
      181 taccgaggtt tccggattct gcgtggtgaa ccataccaca aatgatctgg aattgtgtgg
      241 caaaaacgtg gggggcggag gagtgtgtac tgcacctaat cactccccct gatgatttac
      301 ttttcctact tcagagtacc cgatactctg tgatcagatt tgttccaacc atgcgtgttc
      361 ctgaaagttc aagccggatc atctgatcct ccgagtcaat atgcatccct ccaaccagcg
      421 atggagtatc atttcctccc acaataaacg gaagatggat taatcggatc tcatccacca
      481 gatggtgatg cagcatatgc cagtttagtg tcggtcctcc ctcaattatc agctttctca
      541 ctgaaaattc ccggtataaa atatccatca gcagagggag gtccactttt tctttaccgg
      601 caacaatgac agacacgccc ttttcctgaa gggccgcaac gcgatctttt ggtgctgctg
      661 ctgacacggc gacaactgtc ggaacagttg atgtgtccag tacgtttgca tccaacggga
      721 ggtctgccat gcttgaggga atcacccgca gaggactttt tcccgggacc atacgcactg
      781 tcaggaatga gttatcgatt ttaattgtgg atgatccaac catgatggca tcataagcgg
      841 cacgcgtctc atgaagcaga atttccgcct catgcgacat atgtttcatc aaaattttgc
      901 tggacgcacc gcgctttagg gtaagtttcc catctgctgt gatctcagag atcattaaca
      961 cgtggggttt atgcagtatc atattcatga agtatttcct cttgattcat ataggaataa
     1021 aagctaaaga atatttggta tggaaatccc ggtcaaacgt gcatcatttc cactcccgtc
     1081 agatatctga tgccagcagg ttatcatgaa tattaccgct cttcgtccac ggctgattgg
     1141 ctgtattgat ccaattggca atgccttcgt ccggattggg cttaaaccaa atcagattac
     1201 attcctctcc cttatttttg gtattgcctg tgctgtctgt tatatgcagc ggctgtttct
     1261 ccttgggagt atcctgcttg gggtctctgc aatccttgat ctcgtcgacg gaaacgttgc
     1321 ccggaagacc aatcgtaaga gtgattttgg tgcagtactt gactggatca ttgacaaata
     1381 cgtggacgga attgttcttc ttgggattgg tctctctggg actgctgtca tctctcagtt
     1441 cctcaccgtc cctgcctggt ctgataccgc aatcgttggc cttgctatta tcggctccct
     1501 catgaatacc ttcatcaaac cggtaaccta tgctgaaatc ggctacacta aaaaggaaga
     1561 tggtaaaatc agtgatcctt tggaagggat tggatttttc ggcagacctg aaactatcat
     1621 ctgtctcatc ctgtttgggc tgatcagcca gatttggata gccgtaatcc ttattgctgt
     1681 ctgtacaaat ctctctgctt tacaaagaat tctctacctt gcacgccgcc acggttccta
     1741 caaggaagag tgaaaataag atatttttat tttttcagag ctgcattcaa tgcatttttt
     1801 gtaattccgc gcagaatgac gttaccactc tttgatgcct cggtaagtgc tacaacaacc
     1861 tcatcaccac caatctcgcc cagtacggtc gctgctgcag cctgaacacc gcgctttgga
     1921 tcatgcagac ggtcaataag tgcggcaacc acatcaggcc ttcgcgatga tgcgagtgct
     1981 gtaaccgcag cccgtcgaac ccccggtact gaatcagtta gtagcggcaa caacacatcc
     2041 tcttggagtt cttcctgttt tcccagtgat tctgccgccg ccatacgaac tgaggcatct
     2101 gtatccccgc ataacctggc aagaagtttt tgtgatgcga ctactgatgc accgacacgc
     2161 cggacctgtg gacttggatc ctctgtaaat tcctcaggaa ccaaggggcc aagcactgca
     2221 actgctgcag cacgaatttc cggaaaatcg gtgtcatgca ggcattctaa aacagttact
     2281 atatcttcag gagacaactc ccgtccccga agaccctcca gcgcagcaag acgaatctgg
     2341 ggctcatctt tcagtgatgc aagaagtaga ggaaccactc tcttctccga gtatacacca
     2401 agggataata ttgcaacttc acgaatgtct ggattttttg caccagctgc ctggatcaga
     2461 gggtcgattg cctctggtct ccggaactgt cctagtgcct gaactgcgga aaactggagt
     2521 tcttcgtttt cggaagatac tgcagctacc agtgcatcac acgcaggttt ttccccttcc
     2581 ataaacaaaa gcagcgcagc ctcgcgcaca gccatatcag catcatggag ataggcaaga
//
LOCUS       DALUAJ010000156         1629 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_28034,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000156 DALUAJ010000000
VERSION     DALUAJ010000156.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28034"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="O0X37_03300"
     CDS             complement(<1..333)
                     /locus_tag="O0X37_03300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK61983.1"
                     /translation="MYTTVTDSSHLKKLLAVIALAIFIDSLDGSIVNIALPTIAEGFS
                     VDMSTAAWVIMAYFLFLVGLIPLFGKIADHGKLQEVFCAGFVVFTIGSVVCGFAPDLL
                     MLVAARAVQ"
     gene            complement(334..1347)
                     /locus_tag="O0X37_03305"
     CDS             complement(334..1347)
                     /locus_tag="O0X37_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJK61984.1"
                     /translation="MTDPVSVQTAERLTAERKFTEAADVYRSLLEVEENKTDAGLWCG
                     LGRVLMLDQQFYDAIDAFTTAANFEPKNPKVLAELGDALAAVHEYEEAKLWFEKAASL
                     EDNIRYQLRAGDMLAYLGKYDEALVYYTLLSSKYPENADLLHNKGKVLHHLGRTSDAM
                     NTILEEIRLRKEMAERSPDAQSYAKLAAAYKRIGLWQEAEEEYSTAVRLAPENPEYHM
                     FLGSARVMNGKPAEGVAEYETAAEISGENFSLLSEIAESATKFKMYDAAIRIYTRALA
                     VRNINGDAWAGIAYALLMMNQKDDARAFFEMAKATGSMRELPWADKLHKSYKTEALDQ
                     AFS"
     gene            1465..>1629
                     /locus_tag="O0X37_03310"
     CDS             1465..>1629
                     /locus_tag="O0X37_03310"
                     /inference="COORDINATES: protein motif:HMM:NF001220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HJK61985.1"
                     /translation="MTKTIITVVGKDTVGIIAKVCSYLAENNVNVEDISQTIVQGYFN
                     MMMIADATASP"
BASE COUNT          433 a          455 c          366 g          375 t
ORIGIN      
        1 ctgtactgcc cgggcagcca ccagcatcag cagatccggt gcaaaaccac agacaacgga
       61 tccgatcgta aacaccacaa accctgcaca gaacacctcc tgcaacttcc cgtggtccgc
      121 aatttttcca aacagcggaa tcagacccac aagaaacaaa aaataggcca taatcaccca
      181 tgccgccgtt gacatatcca cggaaaatcc ttccgcaatc gtcggcaggg caatattcac
      241 aatcgaaccg tccagcgaat caataaaaat cgccagagct atcaccgcaa gcagcttctt
      301 taggtgggac gaatcagtaa ccgtcgtgta catctaggag aacgcctgat ccagtgcctc
      361 ggtcttgtaa ctcttatgca gcttatcagc ccacggaagt tcccgcattg atcccgttgc
      421 ctttgccatc tcgaaaaatg cccgggcatc atccttttgg ttcatcatca gaagagcata
      481 ggcaatacct gcccacgcat caccgttgat gtttctgacc gcaagtgccc gggtgtaaat
      541 ccggatcgct gcatcgtaca tcttaaactt cgtcgcagac tccgcaatct cggacagaag
      601 tgaaaagttc tctccggaaa tctcagcggc agtttcatac tcggcaaccc cttcggcagg
      661 ttttccgttc atcacacggg cggaaccaag gaacatatga tactcaggat tctccggcgc
      721 aagacggaca gccgtggagt attcctcctc tgcctcctgc cacagtccga tacgcttgta
      781 ggctgccgca agttttgcat aggattgtgc atccggtgaa cgctccgcca tctcttttct
      841 gagccggatc tcctccagaa ttgtgttcat cgcatcgctg gttctaccaa ggtgatggag
      901 aactttccct ttgttgtgca gaagatctgc attctccgga tactttgagg aaagaagcgt
      961 atagtagacc aaagcctcat catacttccc aagataggca agcatatcac ccgcccggag
     1021 ctgataacgg atattgtcct caagggatgc agctttctca aaccacagct ttgcctcctc
     1081 atactcatgc acggctgcaa gagcatctcc cagttctgcc agaactttcg gatttttcgg
     1141 ctcaaaatta gcagcagtcg tgaacgcatc aattgcatcg taaaactgct gatccagcat
     1201 cagaacacgg ccaaggccgc accagagccc agcatcggtt ttgttctcct caacctccag
     1261 aaggctccga tagacatctg cggcctccgt gaatttccgt tctgccgtca ggcgttctgc
     1321 tgtctgcact gatacaggat ctgtcataga tctgttattg gcaggctgaa gagaaaaaga
     1381 gtactgactg agacgggcaa caacctattt gacgcgggaa agcgaatgaa tatacgacat
     1441 ttgaaccggt gaggaatatc gaggatgaca aagaccatta ttactgttgt aggaaaggac
     1501 acggtgggaa ttattgcaaa ggtgtgcagt taccttgctg aaaacaatgt gaacgttgag
     1561 gatatctccc agaccattgt acagggctac ttcaacatga tgatgatcgc cgacgcaacc
     1621 gcatcaccc
//
LOCUS       DALUAJ010000157         1844 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_28341,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000157 DALUAJ010000000
VERSION     DALUAJ010000157.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28341"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="O0X37_03315"
     CDS             <1..1089
                     /locus_tag="O0X37_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HJK61986.1"
                     /translation="DHIGAVQKLAHRYNCPIIATPYTTELIKQQIEGERKFSVTNKTF
                     ALKAGGKYTISQHLTLEFVRVQHSIIDSVMAVLHTPAGCVVYANDFKLDMTPVIGEAP
                     DLARMRAIGKEGVKVLIVESLNLDDRGYAPSEQVARLRVRDAITRCEDDKNAIIVSTF
                     ASQIARIKTITECAREIDRIPILLGRSMERYNGTAEMMKQVAFPQGTSIYGNRKTTDR
                     MLRRVAKEGKDKFLLVVTGHQGEPGSMLSRMASGDTPFKIEKGDKVMFSANIIPNPVN
                     YAQRAEVDRLLLRQGARIFDGLHVTGHAFYQEHYDLISMLNPEHIVPSHGPFDMKGGY
                     VTLGREFGYALNKEIHIMQNGDRLVLPK"
     gene            1113..>1844
                     /locus_tag="O0X37_03320"
     CDS             1113..>1844
                     /locus_tag="O0X37_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HJK61987.1"
                     /translation="MELSEYLKSVAAKVDQEADEYSKATDTTLQKASAHLLLGGGKRL
                     RPAMVLLAADAVRKGASTDVFSAALALEWTHTFTLVHDDIMDGDSLRRGRPTVHVVWD
                     EPTAILAGDVLYAKAFEYLCSVKNASAESKVAAVQMLSHACYEICEGQQEDVSFETRN
                     DVTADEYLEMVRKKTGVLYAAAAGIGAALAGGDMKQVSAFYTLGLNTGIAFQIQDDII
                     DLMTPPEISGKDQASDLRENKQTILA"
BASE COUNT          446 a          461 c          528 g          409 t
ORIGIN      
        1 gaccacatcg gtgcagtaca gaaacttgcc caccgctata actgcccgat catcgcaacc
       61 ccgtacacga ctgaactgat taaacagcag attgaaggcg agcgcaagtt ctcggtgacg
      121 aacaaaacgt ttgcgctgaa agcaggcggc aaatatacca tctcccagca cctgacgctg
      181 gagttcgtcc gtgtccagca cagtattatc gactccgtta tggcggtgct tcacacgcca
      241 gcgggttgtg tcgtgtatgc caacgacttc aagctggata tgacaccggt gatcggcgaa
      301 gcacccgacc ttgcacgcat gcgtgcgatc ggtaaggagg gtgtgaaggt tctgatcgtt
      361 gagtcgctga accttgatga ccgcgggtat gccccaagcg aacaggttgc ccgtctgcgg
      421 gtgcgggatg cgatcacgcg ttgtgaggat gataagaacg cgatcatcgt ctcgaccttt
      481 gcttcacaga ttgcaagaat caaaaccatc accgagtgtg cccgcgagat tgaccgcatc
      541 cctattcttc tggggagatc catggaacgg tacaacggaa ccgccgagat gatgaagcag
      601 gttgccttcc cgcagggaac aagtatctac ggcaaccgca agacaaccga ccggatgctg
      661 cgccgtgtgg caaaagaagg aaaggacaag ttcctgcttg tggttaccgg tcaccaggga
      721 gagccgggtt cgatgctttc ccgcatggct tccggtgaca ccccgttcaa gattgagaag
      781 ggcgataagg tgatgttttc ggcaaacatc attccaaatc cggtgaacta tgcccagcgt
      841 gcagaggttg accggcttct gctgcggcag ggtgcaagaa tctttgatgg tcttcatgta
      901 acgggtcacg cgttttatca ggagcattat gatctgatct caatgctgaa tccggaacac
      961 attgttccgt cgcacggacc gttcgacatg aagggcgggt acgtgaccct tggccgtgag
     1021 ttcggttatg cgctgaacaa agagattcac attatgcaga acggagaccg tttggttctt
     1081 ccgaaataac caaaaaattg agagtgtgta ttatggagtt atccgagtat ctgaaatccg
     1141 tcgccgcgaa agttgatcag gaggcagacg agtacagcaa agcgactgac acaaccctgc
     1201 agaaagcatc cgcacatctg ctcctcggcg gaggaaaacg ccttcgtccg gcaatggttc
     1261 tgcttgcagc agatgctgtc cgcaaaggtg catccacaga tgttttctcc gcggctcttg
     1321 cccttgagtg gacacatacg ttcacgctgg tgcatgacga tatcatggac ggggactcac
     1381 tgcgccgtgg aagaccaacc gtgcatgtgg tctgggatga accgacggca attcttgcag
     1441 gcgatgtttt gtatgcgaag gcgtttgagt acctgtgttc tgtgaagaat gcaagtgccg
     1501 agtcaaaggt tgcagcggtc cagatgctgt cgcacgcctg ttatgagatc tgtgaaggtc
     1561 agcaggagga tgtgtccttt gagacccgga atgatgttac cgcagacgag tatcttgaga
     1621 tggtcagaaa gaagaccggc gttctgtatg cggctgctgc gggaattggt gcggcacttg
     1681 ccggtggaga catgaagcag gtctctgcgt tttacacgct tggcctgaac accggtattg
     1741 cgttccagat ccaagatgac atcatcgatc tgatgacgcc tcctgagatc tccggaaaag
     1801 atcaggcatc cgatctgcgc gagaacaaac agacgattct ggcc
//
LOCUS       DALUAJ010000158         3064 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_28395,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000158 DALUAJ010000000
VERSION     DALUAJ010000158.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28395"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            21..1031
                     /locus_tag="O0X37_03325"
     CDS             21..1031
                     /locus_tag="O0X37_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HJK61988.1"
                     /translation="MEIRKVQVTGGSSYVISLPKGWVRQQKIEKNDPVGVIAQADGTL
                     LLTPNLTYDSTTRIREFSLKDYPVPDMLLRSLIGAYISGFTTIRITSAGRIPPKVRQV
                     VRKFTQMTIGQEVAEESDNSIILKDILNPSEMPFENTIRRMYVIVKAMHEDALYALEQ
                     GRRELAEDVSARDTDVDRLHWLVHRQFSLIVTNPALSRRMEIAPAKAATYYQVSRIIE
                     RVGDHAVAISEAALVLIDMRVDHTVVAKITKAGEASLDIFNRSIKAIYANSIPEANAI
                     IEAADDLLPEYHTLSAMTQKMKVKEAVAMTTIANSMHRISEYSSDIAEILINQMVGTD
                     GT"
     gene            1163..1759
                     /locus_tag="O0X37_03330"
     CDS             1163..1759
                     /locus_tag="O0X37_03330"
                     /inference="COORDINATES: protein motif:HMM:NF040019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HJK61989.1"
                     /translation="MFIMATGIALSIRADMGITPISSLPYVVSLGVPLTVGMLTILLH
                     AGFLLIEFLLMPREFEPIQLLQLGAAVVFGAFIDLVLAGLAWLVPVSYPEQILLVVLS
                     CALLGVGICFEVAPNVLMMASEGMLTAAVRRFHVEFGTIKVALDVTLVVLSIILSLAM
                     FSTVYGIREGTVIAAVLVGIFVKLFKLPVYAATQKLIG"
     gene            complement(2072..2536)
                     /locus_tag="O0X37_03335"
     CDS             complement(2072..2536)
                     /locus_tag="O0X37_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK61990.1"
                     /translation="MAGLPMVKEILAQSVKKPVTNLFPAVRLPKSITGFLGSVAEGKA
                     EIVPPVETPPAFRGKIAYDRSACNGCGLCLKVCPAHAIDQVVYPPVTVQVTDAEGNVT
                     EKVKKEKRIRIYVSSCIFCGQCTDICAKNALSLQNEFLLATEDRFAESQIVE"
     gene            complement(2538..>3064)
                     /locus_tag="O0X37_03340"
     CDS             complement(2538..>3064)
                     /locus_tag="O0X37_03340"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HJK61991.1"
                     /translation="AAGVSNPFSMLTLMQISVWDVVGPLGILALLILLVMMAWVTPGE
                     LSKIPFDSPEAETELAGGILVEYSGRNLGLFTLSQSVKTVAMSAFGIILLLPWNLSPI
                     LGLSGLSAGAVDLVFFVLKVLFVVIVSVTVVRTMMARFRITQVVGLYWLVLGVLGALA
                     IILLMADAFLLGVI"
BASE COUNT          839 a          748 c          776 g          701 t
ORIGIN      
        1 acatagagta ttgcagatct atggaaattc ggaaagtgca ggttaccggg ggttcgtcat
       61 atgtgatctc cctgccaaaa ggatgggtac gccagcagaa aatcgagaag aatgatcctg
      121 tcggtgtaat tgcccaggcg gacgggacac tgcttctcac accaaatctg acttatgaca
      181 gtacgacacg gatccgggag ttttccctaa aagactatcc ggttccagac atgctgctca
      241 gatccctgat cggtgcatat atttccggat ttaccaccat tcgcataaca tctgctggaa
      301 gaatcccgcc aaaagtgcgg caggtagtac ggaagtttac acagatgacc attggccagg
      361 aggttgccga ggagagtgac aacagcatca tcctaaaaga tatcctgaac ccttccgaga
      421 tgccgtttga gaatacgatc cgccggatgt atgtaattgt aaaggcgatg cacgaggacg
      481 ctctgtatgc actggaacag ggacgtcgtg agctcgccga ggatgtctct gcccgtgaca
      541 cggatgtgga tcgcctgcac tggctggtac atcggcaatt ttcgctgatt gtcacaaatc
      601 cagccctctc gcgaagaatg gagattgccc ccgcaaaggc tgcaacctac tatcaggttt
      661 cacgaatcat tgaacgagtg ggagatcacg ctgtggcaat ctctgaggct gcactggtgt
      721 taattgatat gcgggttgat cacacagttg tcgcaaagat aacaaaagca ggtgaagcat
      781 ccttggacat cttcaacagg agcatcaagg cgatttatgc aaacagtatc cctgaggcaa
      841 acgcaatcat tgaggcagct gatgatctgc tgccggagta tcacacactc tctgcgatga
      901 cccagaagat gaaggtaaaa gaggcggttg caatgaccac aattgcaaac agtatgcacc
      961 ggatcagtga gtacagctcg gacattgcag agatcctgat caatcagatg gtcggaaccg
     1021 acggaacctg agtacacagc aaaagagtag aactcacgca acaagaaaaa ttcacaaata
     1081 ctttcgccgc aaatatagat ccacgcaacc gaaatcagta tatttcaaat tagcccgaaa
     1141 atatggtgtt ttcatcttcg ggctgttcat tatggctacc ggtatcgcct tatcgattcg
     1201 tgcagatatg gggattacgc ctatatccag tttgccgtat gttgtgagtc ttggtgttcc
     1261 actgacggtt gggatgctga caattctgct gcatgccggg tttctgctga ttgagtttct
     1321 gttgatgccg cgggagtttg aaccgatcca gttgcttcag ctgggagctg cggtggtgtt
     1381 tggggcgttt attgatctgg ttcttgcagg tcttgcctgg ctggtaccgg tcagttatcc
     1441 ggaacagata ctgcttgttg tgctgagctg tgccctgctt ggtgtcggta tctgttttga
     1501 ggtggcacca aacgtactga tgatggcaag cgaaggtatg cttaccgcag cagtccgcag
     1561 gttccatgtg gagttcggga caataaaggt cgctctggat gtaacgttag tggtgctctc
     1621 aattattctg tcacttgcaa tgttttccac agtgtatggc atccgggaag gaacagtgat
     1681 agccgcagtt ctggtgggta tctttgtgaa actgttcaaa ctgccggtct atgcggcaac
     1741 acaaaaactc atcggctgag aaaagctctg caaggtatct gccagacttc tgccaggtga
     1801 aattccgcat attttttgta aaattggaaa aagacgaaag acagattgat ttcgctaggc
     1861 tgctagcttg aaacgatatc ccggacctta ccagaaaaat ccgactgcta atttttgaat
     1921 gcccatagag atagaggcag atcacaccaa aggttggcaa gctagcagaa aacgatattt
     1981 tttcgttaaa attaggaaat acagaattat tcaagaaatc aggttggcac aaaccagaca
     2041 ctgggttagc agatcaaaaa aggagaatga gttactccac aatctgggac tcggcaaacc
     2101 gatcctcagt tgcaagcaga aactcattct gaagagacaa cgcattcttt gcgcagatat
     2161 cagtacactg tccgcagaag atacagctgg aaacataaat ccggatacgt ttctcctttt
     2221 tcaccttctc ggtaacattg ccctctgcat cggttacctg tacggtcacc ggcggataga
     2281 caacctgatc gatcgcatgt gcggggcaga ccttcaaaca caggccgcag ccgttacagg
     2341 cagatctatc atacgcaatc tttccgcgga atgccggcgg tgtctcaacc ggaggtacaa
     2401 tctccgcttt accttccgca accgagccga ggaaaccggt gatcgatttc ggcaggcgta
     2461 ctgccgggaa cagattggtc accggctttt tcaccgactg tgcaagaatc tcctttacca
     2521 tcggaagccc agccatctca gatcaccccc agaaggaatg catccgccat aagcagaatg
     2581 attgccagag caccgagcac gccaagcacc agccagtaca ggccgacaac ctgcgtaatt
     2641 ctgaaacggg ccatcatcgt tctgaccacg gttacagaaa cgatcaccac aaacagtacc
     2701 ttcaggacaa agaagaccag atccactgca ccggcagaca gacccgacag tccaaggatc
     2761 ggggacaggt tccacggaag cagcagaatg atgccgaacg cagacatggc aacggttttc
     2821 actgactggg aaagggtgaa aagaccgagg tttcgaccgg agtactccac aagaatacca
     2881 ccggcaagct ccgtctccgc ttccggtgaa tcgaacggaa tctttgacag ctcgcccggt
     2941 gttacccagg ccatcatcac aagaaggatg agaagtgcaa ggataccaag cgggccgacc
     3001 acatcccaga ccgagatctg catcagtgtc agcatcgaaa acgggtttga tacgcctgca
     3061 gcca
//
LOCUS       DALUAJ010000159         5002 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_28775,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000159 DALUAJ010000000
VERSION     DALUAJ010000159.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28775"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="O0X37_03345"
     CDS             complement(<1..455)
                     /locus_tag="O0X37_03345"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HJK61992.1"
                     /translation="MTFAADILSLLFHPKEFFTDTSRTHSLKLPLLYTAVYGIFAAVL
                     GYQTTAASAELIGMPDYAGVLGAVGAVTGFIMAFIVWVFVAVFFFVFIKFITHTATGF
                     KPFLVVTGYGSLPVLIGTVLMLLAGAVTPGVFDGWAGYLLNAAVLLWCMP"
     gene            complement(505..1395)
                     /locus_tag="O0X37_03350"
     CDS             complement(505..1395)
                     /locus_tag="O0X37_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJK61993.1"
                     /translation="MVDRTIENFDCGHRACGGCGASSAVRMILKGAGENTIVVSPTGC
                     LEVFSTPYPETAWKTPWIHSLFENASAVASGIEASLKKQGRLGKEKILVIGGDGATVD
                     IGMLCISGAFERGHDFTYVCYDNEAYMNTGIQRSGATPYDADTTTSPSGACSTGNNRP
                     KKDLPQILVAHGSPYVATATMAYPMDLMKKVEKAMNTPGPCYIQVHAPCCTGWGFDGS
                     KTMEIGRMAVQCGLWPCYEMVNGELVSAKKVKRVPVDEYLKAQKRFRHLFKPTRNDEE
                     IAKIQKIADANAAKYGIDIE"
     gene            complement(1396..2553)
                     /gene="porA"
                     /locus_tag="O0X37_03355"
     CDS             complement(1396..2553)
                     /gene="porA"
                     /locus_tag="O0X37_03355"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorA"
                     /protein_id="HJK61994.1"
                     /translation="MTLQIMEGSIAVAETVRLCRPQVISAYPITPQTHIVEGLASIVA
                     DGRLDAEYICVESEFSALSCCIGASAAGSRVYSATTSQGLALMGEVVFNAAGMRLPIV
                     MGIANRAISAPLSIWNDQQDSIMMRDSGWLQFYAEDNQEAVDLHVLAYKICEDHNILL
                     PAFVCFDGFILSHVFEPVDIPSQEEVDEFLGPFTPYQKLDVNDPISFGMYATPEYYLE
                     FRYEHDQAVHRAADAMRKYGKAFGEKFGRDYSSLVEGYKLEDAEIAIVAMGSICGTTK
                     DAIDEMRAEGKKVGLLKIRCFRPFPAEDIAKALAHVNTIAVLDKNVSLGAKGAVAIEV
                     KEACYGSGIPVYDYILGLGGRDVRKKDIKHIVELAEKGSGDMFYGLREELL"
     gene            complement(2554..2820)
                     /locus_tag="O0X37_03360"
     CDS             complement(2554..2820)
                     /locus_tag="O0X37_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK61995.1"
                     /translation="MPLGIGCTARPGQSRNNKTGSWRVFYPILDKEKCTKCGTCQLIC
                     PEGCITQLPDKTYEIDLDFCKGCGMCAEECPDKAKAITMHKEEK"
     gene            complement(2820..3365)
                     /locus_tag="O0X37_03365"
     CDS             complement(2820..3365)
                     /locus_tag="O0X37_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase subunit
                     gamma"
                     /protein_id="HJK61996.1"
                     /translation="MKELRIHGRGGQGSVTAAELIATAAFAGGVHAQAFPAFGVERRG
                     APVQAFVRFSNEKIRLRSQVYEPDYIIVQDSTLIRDVNVFAGMSEGGIAIINTEKKGE
                     YALPEGVRLIVIDATQIALEEIGLPITNTTLMGAFAAASGEITLDALKDAIEERFPGK
                     LAETNFAAAKRAYNMVLEGAV"
     gene            complement(3543..4430)
                     /locus_tag="O0X37_03370"
     CDS             complement(3543..4430)
                     /locus_tag="O0X37_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 12 protein"
                     /protein_id="HJK61997.1"
                     /translation="MYIGIDHGTTSLRFAADNGKRFKMSREAAKEFTIEDLQKLGPVE
                     DIAGIALCYSMGDNFTQITPVDKLKNRGVITRDGAGEHIGGGTRVFDAVRESGIPAIA
                     IPGIHRGSDTDPRFKIYSHQTSPEKLGIAYLVSETLGDTFIVSDISSNTVSLLIANGK
                     VIGAFDACVFAPGTRHGALDVDAIRKVDAGEITANEAFTTAGVMYHLEEKYQNPTVAM
                     WAAMECASLSLLAPDASIALAGSLAPELAEEISALLQKPVIVYDEWAASDGLAKIARD
                     VFSGAKQILGIPVSPNLRI"
     gene            4525..>5002
                     /locus_tag="O0X37_03375"
     CDS             4525..>5002
                     /locus_tag="O0X37_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HJK61998.1"
                     /translation="MAETRTHYTILGVPQNASPEMIKKAYVLLVKQYHPDRAGSDEAK
                     QEEYNERLLEIMASYEILSDPVKRAEYDAVLSGTESTSGTPRGKKMCGMPVKGGDIET
                     DYPISMAIAAYGKGKIPMKVAGERVVVKVYPGVRRYRIEGYGVPVEPGKPRGDLYVN"
BASE COUNT         1146 a         1283 c         1311 g         1262 t
ORIGIN      
        1 ggcatacacc agaggaggac cgcagcattc agaagatatc ctgcccatcc gtcaaagact
       61 cccggtgtga ctgctcctgc aagcagcata agtaccgttc cgatcagaac cggaagggat
      121 ccgtacccgg taacgacaag gaatggtttg aaacctgtag ctgtgtgcgt aatgaacttg
      181 ataaacacaa agaaaaatac cgcgacaaaa acccagacta tgaaggccat gataaacccg
      241 gttaccgcac caactgcacc gagtacccct gcatagtcag gcatgccgat gagttctgcg
      301 gatgccgcag ttgtctgata cccgagaact gctgcaaaaa ttccgtagac tgctgtatag
      361 agcaggggaa gtttgagaga gtgcgtacgg gacgtatctg taaagaactc ttttggatgg
      421 aatagcaggg agaggatatc tgctgcaaac gtcattacag tctatattcg ttctctttgg
      481 atatgagtac ttggaaaaaa agagttattc aatgtcaata ccgtactttg cggcgttggc
      541 atcggcaatt ttctggatct ttgcgatctc ttcatcattt ctggtcggtt tgaacaggtg
      601 gcggaaccgc ttctgggctt tgagatactc atcgaccggt acacgcttga ccttctttgc
      661 ggagacaagt tcaccgttca ccatctcata gcacggccac agaccgcact ggaccgccat
      721 tcttccgatc tccatcgtct ttgatccgtc aaatccccat ccggtacagc agggcgcgtg
      781 tacctggatg taacagggac cgggagtgtt catagctttc tcgacttttt tcatcaggtc
      841 catcgggtat gccattgttg cagttgccac atacggagat ccgtgtgcga ccagaatctg
      901 cgggagatcc ttcttgggcc ggttgtttcc ggttgagcat gcaccggaag gtgacgtggt
      961 ggtgtctgcg tcgtacggcg ttgcaccgga acgctggata cctgtgttca tgtaggcttc
     1021 attgtcatag cagacatagg tgaagtcatg accgcgctcg aacgctccgg agatacagag
     1081 cataccgata tctacagtgg cgccgtctcc gccgatgacg aggatctttt cctttccgag
     1141 gcgtccctgc ttctttaggg atgcctcaat accggacgca accgcggatg cgttctcgaa
     1201 cagtgagtga atccagggtg tcttccatgc ggtctccggg tatggagtgg agaacacctc
     1261 aagacatccg gttggactca caacgatggt gttttctccg gctcctttca ggatcatccg
     1321 aaccgcagaa gatgcaccgc atccgccgca tgcacggtga ccgcagtcga agttttcaat
     1381 tgtacgatct accatttata acaactcctc gcgcagtccg tagaacatat cgccggaacc
     1441 tttctcggca agttctacaa tgtgtttgat atccttcttg cgtacgtcgc ggccgccaag
     1501 accgaggatg tagtcataga ccggaatgcc ggaaccgtag caggcttcct tgacctcaat
     1561 agctacagct ccttttgcac caagactgac attcttgtcc agcacggcaa tggtgtttac
     1621 atgggcaagt gcttttgcaa tgtcctctgc agggaacgga cggaagcagc ggatctttaa
     1681 gagaccgacc ttcttaccct ccgcacgcat ctcatcgatt gcatctttgg tggtaccgca
     1741 gatggaaccc attgcaacta ttgcgatctc cgcgtcctcg agtttgtatc cttctacaag
     1801 gctggagtag tcacggccga acttttcgcc aaaggctttg ccgtacttgc gcatcgcatc
     1861 tgctgcacgg tgtactgcct gatcgtgttc gtaccggaac tcaaggtagt actcaggtgt
     1921 tgcgtacata ccaaacgaga tcggatcgtt cacgtcaagt ttctggtacg gagtgaacgg
     1981 accaaggaac tcgtccacct cttcctgcga agggatgtca accggctcga atacgtgtga
     2041 aagaatgaat ccgtcaaagc agacgaatgc cggaagcagg atgttgtggt cttcacagat
     2101 cttgtaggca agaacatgca gatcaactgc ttcctgattg tcctctgcat agaactgcag
     2161 ccagccggaa tctctcatca taatggagtc ctgctgatca ttccagatcg ataacggtgc
     2221 agagattgca cggtttgcaa tgcccatcac aatcgggagc cgcattccag cagcgttaaa
     2281 gacaacctct cccatcagtg caagaccctg ggatgtggtt gcagagtaca cacggctgcc
     2341 ggcagcagat gcgccgatac agcaggaaag tgcggagaac tccgactcca cacagatgta
     2401 ctcggcatca agccgtccgt ccgcaacgat ggatgcgagt ccttctacaa tgtgggtctg
     2461 cggggtgatc gggtatgcgg agatgacctg cgggcggcag agccgtacgg tctctgcaac
     2521 agcaatggac ccctccataa tctgaagagt catttatttt tcctccttgt gcatagtgat
     2581 tgcctttgcc ttgtcgggac actcttccgc acacatgccg cagcctttgc agaagtcgag
     2641 atcgatctcg taggttttgt ctgggagctg ggtgatgcag ccttccggac agatcagctg
     2701 acatgttccg cattttgtac atttttcctt gtcaaggatc gggtagaaaa cccgccagga
     2761 tccggttttg ttgtttctgg actgtcccgg ccgtgctgta catcctattc caagcggcat
     2821 tatactgctc cctccaaaac catattgtat gcccgttttg ctgcggcgaa gttcgtctca
     2881 gcaagctttc cggggaaccg ttcttcgatt gcatccttga gtgcatcaag ggtgatctca
     2941 ccgctggccg cagcaaatgc tcccatcagc gtagtattgg tgatcgggag tccgatctct
     3001 tcaagggcaa tctgtgttgc atcaataacg atcagtctga caccttccgg aagggcatac
     3061 tctcctttct tttcggtgtt gatgatggca attcctcctt cggacattcc tgcaaagacg
     3121 ttgacgtcgc gaatgagggt gctgtcctgt acaataatat aatccggctc gtagacctgg
     3181 cttctcagcc ggatcttctc gttgctgaac cgcacgaatg cctgaacggg tgcaccgcgg
     3241 cgttcaacac caaatgctgg aaacgcctgg gcgtgcacac caccggcaaa agctgcggtt
     3301 gcaataagtt ccgcagctgt gacggacccc tgtccgcctc tcccatggat gcgtagttct
     3361 ttcacgtgaa taaccagtat aatttatcac gattaatcaa tatgaattta gtgtttccac
     3421 cgatgtctct tattttggga tattttctaa aaatagtggt gaattatctg ttttttggga
     3481 ttggttgttg tattcttttt tatctcttgg gcggctgaat cactgtccgc cgatttgtgg
     3541 tttcagatcc tgaggttcgg agagacaggg attccaagaa tctgttttgc tccggaaaag
     3601 acatcacggg cgattttcgc aagtccgtcg gaggctgccc actcgtcata gacgataacc
     3661 ggtttttgca gaagcgcaga gatctcctcg gcaagttcgg gtgcaagact tccggcaagg
     3721 gcaatggaag catccggtgc aagcagggaa agtgatgcgc attccatcgc tgcccacatg
     3781 gcaacggtcg ggttttggta tttttcctcc agatgataca tcacgcctgc ggtggtgaag
     3841 gcttcgtttg cggttatctc tcctgcgtca acctttctga tagcatcaac gtcaagggca
     3901 ccgtgacggg tgccgggcgc aaacacacag gcatcaaagg cgccgatcac ttttccgttc
     3961 gcgatcagta gcgagacagt gtttgagctg atgtcggata cgatgaaggt atcaccgagt
     4021 gtctcggaga caaggtacgc gataccgagt ttttccgggc tggtctggtg cgagtaaatc
     4081 ttgaaacgcg gatcggtgtc agatcctcgg tgaatacccg gaatggcaat tgccggaatg
     4141 cccgattccc gtaccgcatc aaagacgcgt gttcccccgc caatatgctc tcccgcaccg
     4201 tcgcgggtaa tgacgccgcg gtttttcagt ttgtcaaccg gcgttatctg cgtgaagttg
     4261 tcacccattg agtagcagag ggcaatgcct gcaatatcct caacaggtcc gagtttctgc
     4321 aaatcctcaa ttgtaaactc ttttgctgct tcacgggaca tcttgaaccg tttcccgttg
     4381 tctgcagcaa aacggagtga ggttgtcccg tgatcaatcc caatgtacat gggaattata
     4441 tttggaggaa gtcctgataa tggttggtct cctgtatttt cccagtaaga ttatgatttt
     4501 gtcccgccaa catgatctca tttcatggcc gaaaccagaa ctcactatac tatcctgggt
     4561 gttccgcaga atgcaagccc ggagatgatc aaaaaggcct acgtcttgtt ggtgaagcag
     4621 tatcatccgg atcgtgcggg atctgatgag gcaaaacagg aggagtacaa tgagcgtctg
     4681 cttgagatca tggcaagcta tgagatcctc tctgatccgg tgaagcgtgc cgagtatgat
     4741 gcggtgctga gcggtaccga gtctacatcc ggaaccccgc gcggaaaaaa gatgtgcggt
     4801 atgccggtga agggcgggga tattgaaacg gactatccga tctccatggc tattgctgcc
     4861 tacggcaaag gcaagatccc catgaaggtt gctggtgaac gcgtcgttgt caaggtctat
     4921 ccgggagtcc gccgctaccg catcgaaggg tacggcgttc ctgttgaacc gggaaaaccc
     4981 cgcggtgatc tgtatgtgaa cc
//
LOCUS       DALUAJ010000160         6938 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_28779,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000160 DALUAJ010000000
VERSION     DALUAJ010000160.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6938)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6938)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6938
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28779"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            185..493
                     /gene="rpl12p"
                     /locus_tag="O0X37_03380"
     CDS             185..493
                     /gene="rpl12p"
                     /locus_tag="O0X37_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein P1"
                     /protein_id="HJK61999.1"
                     /translation="MEYIYAALLVHSAGKTVDEESVKAVLSAAGIAVDDSRVKALIAA
                     LDGVDIEEAISKAAAAPVAVAAAGAAAPAAAAEEAAAPEESKEEEEEDAMAGLGALFG
                     "
     gene            complement(706..1899)
                     /gene="thiI"
                     /locus_tag="O0X37_03385"
     CDS             complement(706..1899)
                     /gene="thiI"
                     /locus_tag="O0X37_03385"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HJK62000.1"
                     /translation="METNTAVMVRIGELWLKSEQVKKQFMASLMRNIRASLDAAGIEY
                     KTEEYRGRILIYGDAERIAAVVSRVFGIVDVSICTTCGNSPDEIAAAAVAVADGKLRP
                     GMRFAVRARRQHVGGFTSQELARIVADAVWDQVPDFVVDLEHPEYEIFVEAREYGGIV
                     YDSRIPGQGGLPLGTAGKAMLLLSSGIDSPVAAWLMMRRGVVISGVFMDGGRWAGPAT
                     KDLALDNARILSTWSPGKDFAVWVVDMEPFFAAMTTSCDRHYTCIFCKRFMMRVADAL
                     ASRGKFDALVSGENLGQVASQTIQNMRVITESVKTPILRPILTYDKEETVAIARKIGT
                     FHESPGDTGCRAVPKKPATRSSVELIEKEETKLDLAELIEQACASAELWIARDGTISR
                     KMIET"
     gene            complement(1878..2666)
                     /locus_tag="O0X37_03390"
     CDS             complement(1878..2666)
                     /locus_tag="O0X37_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HJK62001.1"
                     /translation="MNEKSSLIPAKLSSVLQQHAPLLIALSGGQDSLVLLASAKALDL
                     PVDAATIVSEFSVPGEMERASEMCRRLGVCWHPIEVSLLDNPAIRSNPVDRCYLCKHQ
                     LMYPLVVLANELGSTVCDGTHADDLPEERPGYAVLKQLGICSPFADAGIGKSEIQALG
                     DALGIPRIPPSSCLATRIPFGVPITAERLKLIAAAETLLRDQGISGILRVRLIADEAV
                     VEVEAWEMQKALALLGQIEDLGFSHVRVAGYTAGGVERWRQTQQ"
     gene            2814..3458
                     /locus_tag="O0X37_03395"
     CDS             2814..3458
                     /locus_tag="O0X37_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2150 family protein"
                     /protein_id="HJK62002.1"
                     /translation="MAAKKSSKKTAEPEAPAKLFYIFYNQERWDNWLNTLAEADFSGD
                     EDSDEMPEGYRILDGFSDDITLATIKIIRLYQNGRISLEEARTKLRGVEEIVMEELPD
                     SEIAEIVGSMQVSMLVLFAAGQKYLEEAYPPSEEVKNLVKEGRKVFEKDPEKALDLAS
                     SIGAAVINGASCCGKYVKDTDEPTLFDEWLVEVERIADAMKSLKNFDEEAGEAQ"
     gene            3455..3907
                     /locus_tag="O0X37_03400"
     CDS             3455..3907
                     /locus_tag="O0X37_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5814 domain-containing protein"
                     /protein_id="HJK62003.1"
                     /translation="MIAGRARFRYIKKLQRAAGYRLPDGAFHPANLEAITGSMNIDSL
                     DPGTRDQVLRFYKDFLECNCRDSPLCGCPERKFVIAILELREMGLNHKEIHNHLLDEY
                     GIDLFPADILSFLEDSVHLLEAVLSVADLAGQKDLVKLSDEHIRQIAR"
     gene            3957..4727
                     /gene="cofE"
                     /locus_tag="O0X37_03405"
     CDS             3957..4727
                     /gene="cofE"
                     /locus_tag="O0X37_03405"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HJK62004.1"
                     /translation="MNPGFSVYGVATGLLEPGDDIVERVIAGLAKTDAGEIHDGDILL
                     FAESPLSTTEGRNIRLADITPGKEARELAKQYSLDPRIAEIVIAESDEILGGIPGFLL
                     AARGNLVLPNAGVDESNAPDGWVTRLPKDANASAARIRKEILNRTGKEVAVIIIDSRT
                     HAMRLGVSGVAIGCSGILPITDERGKPDLFGHELQVTRRAIADSLASTAELLMGEANE
                     GVPVVLVRGYPYIFAENAVIESIAPDEDLFLGSLMKKE"
     gene            complement(5209..5673)
                     /locus_tag="O0X37_03410"
     CDS             complement(5209..5673)
                     /locus_tag="O0X37_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HJK62005.1"
                     /translation="MVEFDLDFQDAIREVEEKGAKATAAEWLARVEAEYGTAPLIYKK
                     LEDSPEALISHLLYKNAVNQMSSLEPRVVELISLAVGAALRCDHCTAYHMQVAKKMGI
                     PKEEILEAVLVAGLLANSSVLANAYRVVIPDAAKQPCGASCSISGIPAKKEE"
     gene            complement(5676..6614)
                     /locus_tag="O0X37_03415"
     CDS             complement(5676..6614)
                     /locus_tag="O0X37_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanogenesis marker 7 protein"
                     /protein_id="HJK62006.1"
                     /translation="MILVPVTYKGGVYRLDEVVDYIEDLGGYIVQRHNIANEVVLQVL
                     MPQEDIEGLKEFSRPLAGEVQTSPLVGTEIAVVIPSLEIHHLPHSACDVAEYLRSHGS
                     KSNILGMARGFGKRISQINDEERDLINEHDMAIYVLGNFASCIEKKFPKLRRGITVPI
                     ILTGGPSREQLEKQTDPPVEGYVGGLGRFMHRTQTEADIHRLDLVIEEVERVISEKRK
                     ELSHDPLSVSPARIQDMIKQEIPEIEDVYSPSPIAVQLTGLRVKLAYEEYAQRIRDLV
                     IEGDITVGDVADVLPSRMRNYILIRIRPLSETNIVV"
     gene            complement(6611..>6938)
                     /locus_tag="O0X37_03420"
     CDS             complement(6611..>6938)
                     /locus_tag="O0X37_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methanogenesis marker 17 protein"
                     /protein_id="HJK62007.1"
                     /translation="TYLAPMLAMLWKRLGHDNVDQPDRFTVIISNVEIPKDIENWVVI
                     DPAESLFKDLIYAIQVIAPEGFKVRRENYGSDRFSYAASENTLPAEDVDRIIADRFKH
                     MEEELR"
BASE COUNT         1862 a         1814 c         1664 g         1598 t
ORIGIN      
        1 cattccgacc ccatgtgcac agatcgctga ggctcagatt gcaaaggctg tccgtgaggc
       61 acggaacctt ggtgtggaag ccccaatcta cgagaagggc gtcatcgaga tgatcatcag
      121 gaaggcatac agacaagcaa acgccctgaa ggcataatta tcatacaaaa taggtgaaga
      181 taaaatggag tacatttacg cagctctgtt agttcactcc gcaggaaaga ctgtggatga
      241 agaatcggtt aaggcagttc tctctgccgc aggtatcgca gttgacgact cccgtgtaaa
      301 ggcacttatc gctgcacttg acggtgtcga catcgaggaa gcaatctcca aggcagcagc
      361 agcaccggtt gcagttgcag cagcaggtgc agcagcaccg gcagcagcag cagaagaggc
      421 agcagcaccg gaagagagca aggaagagga agaagaggac gccatggcag gccttggcgc
      481 actctttgga taagttcgaa catctccctg ctgagatacg tcgaatgccg cagcaaagat
      541 atgctgtggc attatccata ttttcctgaa attatctgat gaaatttgtt agtacgtttt
      601 ttctgatgca ttttataaca gatcccaaac gggaaaactg tgtaagactt ttatccagaa
      661 agaaaaatat ccgttacaaa aaaaacagca ggatgaaaag aaccatcagg tctcaatcat
      721 ctttcgggaa atagtaccgt ctcgtgcaat ccacaactca gctgatgcac aagcctgttc
      781 aatcagttct gcaagatcca gcttcgtctc ctctttctca ataagttcaa cagaggatct
      841 cgtggcaggt tttttcggaa cagcccggca tccggtatca cccggactct catgaaatgt
      901 acctattttc cgggcaatgg caactgtttc ctctttgtca tacgtaagta tcggccgcag
      961 gattggtgtc ttcacagact cagtaatcac acgcatattc tggatcgtct gtgatgcaac
     1021 ctgcccaaga ttttccccgg aaacaagggc gtcaaacttg ccccgcgatg caagtgcatc
     1081 tgcaacccgc atcataaacc gtttgcagaa gatacacgta taatgtctgt cacaggatgt
     1141 agtcatcgcc gcaaaaaacg gttccatatc aacaacccag actgcaaaat ctttcccggg
     1201 tgaccaggtc gaaagaatgc gtgcattatc aagtgcgaga tcctttgtgg caggccctgc
     1261 ccatcttccg ccgtccatga aaacaccgga aataacgacg ccacgacgca tcatcagcca
     1321 tgctgcaacc ggagagtcga ttcccgagga aagaagaagc attgcctttc ctgccgttcc
     1381 gagaggcaga ccaccctgac cgggaatacg gctgtcatag acaattccac catactcacg
     1441 tgcttcaaca aagatctcgt actcgggatg ttccagatcc accacgaaat ccgggacttg
     1501 gtcccagaca gcatccgcga cgatccgcgc aagctcctga ctggtgaacc cgccaacatg
     1561 ctgacggcgt gcccgtaccg caaaccgcat tcccggacgc agttttccgt cggccacggc
     1621 taccgccgct gcggcaatct catccggcga gtttccgcag gttgtacaga tactcacatc
     1681 cacaatgccg aagacacgtg acacgacagc tgcaatgcgt tccgcatcgc catagatcaa
     1741 aatcctgcca cgatactctt cggtcttgta ctcgatcccc gctgcatcga gtgacgcacg
     1801 aatgttgcgc atcagcgatg ccatgaactg tttcttaacc tgctcactct tcagccagag
     1861 ttccccaatt ctcaccatta ctgctgtgtt tgtctccatc tctccacacc tcctgctgtg
     1921 tatccggcaa cccgtacatg agaaaaacca agatcttcga tctgaccaag aagagccaaa
     1981 gctttctgca tctcccacgc ctcaacctcc acaaccgcct cgtccgcgat gagacgtaca
     2041 cgaagaattc cagagatacc ctgatcacgc agcagggttt ctgctgcggc aatcagtttc
     2101 agacgttctg cggtaatcgg tacgccaaaa ggtatccggg tcgcaagaca tgatgacggc
     2161 ggaatacggg gaatcccaag tgcatctcca agagcctgga tttcggattt tccgataccc
     2221 gcatcagcaa acgggctaca gatcccaagc tgtttcagaa ccgcataacc aggtctctcc
     2281 tccgggagat cgtcagcatg cgtaccatcg cagacagtac tgcccagttc attggcaagt
     2341 acgacaagag gatacatgag ctgatgcttg cagagataac aacggtctac aggatttgat
     2401 cggatcgctg gattgtcaag cagcgaaacc tcgataggat gccaacatac tccaagcctt
     2461 cggcacatct cagaggcccg ttccatctca cctggaaccg aaaactccga cacgatcgtg
     2521 gcggcatcga ccggaagatc aagagctttt gctgatgcaa ggaggacaag actgtcctga
     2581 ccgccggaga gtgctatcag cagtggtgca tgttgctgca aaaccgagga taattttgcc
     2641 ggaatcaaag aggacttttc attcactttg tgaacatcca gaagtactca gtatttgctc
     2701 tgaataaata aatatgcaat ggcttgagaa agggatactc cattgcacac ccagttccga
     2761 caggaatatc acaacatcta taagaatcag ggcagaacta tttacagtga gatatggctg
     2821 caaaaaaatc ttctaaaaaa acagccgagc cagaggcacc ggcaaagctg ttctatatct
     2881 tctataacca ggagcgctgg gacaactggc tgaacaccct tgccgaggct gatttctccg
     2941 gtgatgaaga cagtgatgag atgccggaag gataccgtat tctcgacgga ttctccgacg
     3001 atatcacgct tgctacaatc aagatcatcc gcttgtacca gaacggaaga atttctctgg
     3061 aagaggcacg aaccaagctc cgcggtgttg aggagatcgt gatggaggaa ctgcctgact
     3121 cagaaatagc agagattgtc gggtcgatgc aggtatccat gctggtgctg tttgcagccg
     3181 gacagaaata tttggaagag gcatacccgc caagtgagga ggtcaaaaat ctcgtaaagg
     3241 aagggcgtaa ggttttcgaa aaggatccgg aaaaggcgct ggacctggca tcctccattg
     3301 gagcagccgt tatcaatggt gcctcgtgct gcgggaaata tgtcaaggac accgacgagc
     3361 ctacgctttt tgatgagtgg cttgttgagg tggaacgcat tgccgatgcc atgaagtctc
     3421 tgaagaactt cgacgaggaa gccggagaag cccagtgatc gcaggccgtg cccggttccg
     3481 ctacatcaaa aaacttcagc gggcagcagg ataccgatta ccggatggcg cctttcaccc
     3541 ggcaaatctg gaagccatta ccggttcaat gaacatcgac agtcttgatc ccggaacacg
     3601 ggatcaggtt ctccgatttt acaaagattt tctcgagtgt aactgccgtg actctccgct
     3661 ctgcggatgc ccggagcgaa aatttgtgat cgcgattctg gaacttcgag agatgggact
     3721 taaccataaa gagattcaca atcatctcct tgatgaatac ggaattgatc tcttccccgc
     3781 agatatactc agctttttag aagattccgt tcatctcctg gaagcagtac tcagtgttgc
     3841 ggatcttgcc ggacagaaag atctggtcaa actatctgac gaacatatcc gccagatagc
     3901 ccgctgacgc acattcccat cacgtttttt tgagatacag acacaataga tcatacatga
     3961 atccggggtt ttccgtctat ggggttgcaa caggtcttct tgagccggga gacgacattg
     4021 ttgaacgggt gattgcaggt cttgcgaaaa cagatgcagg agagattcac gacggagata
     4081 ttctcctgtt cgcagagtct cccttgtcca caacggaggg gcggaatatc cggttggccg
     4141 atattacacc cggaaaagaa gccagagaac ttgcaaaaca gtacagtctt gacccgcgaa
     4201 tcgcagagat tgtcattgcg gaaagcgatg agatcctcgg tggtatcccc ggattccttc
     4261 ttgctgcacg gggaaatctg gttcttccaa atgccggggt ggatgagtcc aacgcaccgg
     4321 acggctgggt tacccggctg ccaaaggatg caaatgcaag tgctgcacgc atccggaaag
     4381 agatcctaaa ccgtacagga aaagaggttg cagtaatcat tatagactcg cgtacacatg
     4441 caatgcggct cggtgtttcc ggggttgcaa tcggatgttc tggtattctc cccatcactg
     4501 atgagcgggg aaaacccgat ctcttcggac atgaattaca ggtaacacgc agggcaattg
     4561 ccgattcgct tgcatcaaca gcggagcttc tgatgggaga ggcaaatgaa ggtgtacctg
     4621 tagtgcttgt acgtggctat ccgtacattt ttgcagaaaa tgcagtcatc gagtccattg
     4681 cacccgatga agatcttttc ctcggaagtc tgatgaaaaa agagtgacaa agccacatct
     4741 tcgcacatga aagaggtgaa acggatgtgt cactgatctg aaaaaatatt ttttagttcc
     4801 gcaatgctcc aatcaccact atgccgcaga cgatcagtca cgcccaccag aagatgaacg
     4861 atgatgactc cggctgtaac ccacgacaga aaccttatgc aggtttatga gatcgttttt
     4921 tatccgcaca agtgtcgcca aggaaagttg gatttaccga gccactcatg aggaacgtcg
     4981 tcgtcgaaat tatgttcgag ttgagaaaga taggtctcgt gtctcctctt tactctctgg
     5041 atgatgaatt gtatcagatc tagtacagca ccgggacaga gagtcaccag acctgaacaa
     5101 aatcaaacgc aaaaacgatt acgtatcccc tcaaaaaccg gcatatccca gaaagaacac
     5161 taaaaaattg aagacaggac cctataaaat cctgagtatg atgacaaatt attcttcttt
     5221 tttggcagga attccggaaa tagagcagct tgccccacac ggttgtttgg cagcatcagg
     5281 tatcacaaca cgataggcat ttgccagaac ggatgagttt gcaagaagtc ctgcaaccag
     5341 gacagcctcc aatatctcct ccttgggaat acccatcttc tttgcaacct gcatgtgata
     5401 tgccgtacag tgatcacagc gaagtgctgc ccccactgca aggctgatca attcaacaac
     5461 tcttggttcc agactgctca tctgattcac ggcattctta tagagaagat gtgagatcaa
     5521 cgcctcggga gagtcctcga gttttttgta aattaacggg gcagtcccgt actctgcctc
     5581 aacacgagcc agccattctg ctgcggttgc tttcgcccct ttctcctcaa cctcgcggat
     5641 agcatcctgg aaatccaaat cgaactctac catgattaca ccacaatgtt cgtctctgaa
     5701 aggggacgga tcctgatcag aatataattc cgcatccgtg acggaagaac atcagcaaca
     5761 tcgccgaccg taatgtcgcc ttcaattacc agatcgcgga tacgctgtgc atactcttca
     5821 tatgcaagtt ttacccgcag accggttagt tgtaccgcaa tcggtgacgg ggagtacacg
     5881 tcctcgatct ccgggatctc ctgcttgatc atgtcctgaa tccgtgccgg tgatactgac
     5941 aggggatcgt gggacaactc ttttcgtttc tcggagataa cacgttccac ctcctcaatc
     6001 accagatcaa gccggtgaat atccgcctcg gtctgtgtcc ggtgcatgaa cctcccaaga
     6061 cctccgacat atccttccac cgggggatcg gtctgttttt ccaactgttc acgtgacggc
     6121 ccgccggtca aaatgatcgg cacggtaatt ccgcggcgaa gtttcgggaa cttcttctca
     6181 atacaggatg caaagttgcc aagtacatag atggccatgt catgctcgtt gatcaggtca
     6241 cgctcttcgt cattgatctg tgagatgcgt ttgccaaatc ctctggccat gccgaggatg
     6301 tttgattttg agccgtggct acgcaggtac tcggcaacat cacaggcaga gtgcggcagg
     6361 tggtgaatct caagacttgg aatcacgacc gctatctcgg tcccaaccag cggtgaggtc
     6421 tgcacctctc ctgcgagcgg acgggagaat tccttaagcc cttcaatatc ttcctgcggc
     6481 atcagtacct gcagcacaac ctcgttggca atattgtgcc gctgaacgat gtagcccccg
     6541 agatcctcga tgtagtcaac aacctcatca agccggtaca cacctccctt gtaggtaacc
     6601 ggaacaagaa tcatcgcagc tcctcctcca tgtgtttgaa tctgtcagcg ataatccgat
     6661 caacatcctc agccgggagt gtattctcac ttgccgcata ggagaaacga tcggatccgt
     6721 aattctcacg gcgaactttg aacccctccg gtgcaattac ctggattgca tagatcaaat
     6781 ccttaaacaa cgactccgca ggatcaataa caacccagtt ttcaatatct ttgggaatct
     6841 caacgttgct gatgataacc gtgaacctat ctggctgatc cacgttgtcg tgaccaagac
     6901 gcttccacag catggcaagc attggagcaa gataggtc
//
LOCUS       DALUAJ010000161         4387 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_29376,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000161 DALUAJ010000000
VERSION     DALUAJ010000161.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29376"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="O0X37_03425"
     CDS             complement(<1..299)
                     /locus_tag="O0X37_03425"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834018.1"
                     /note="catalyzes the reversible formation of fructose
                     1,6-bisphosphate from glycerone phosphate and
                     D-glyceraldehyde 3-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HJK62008.1"
                     /translation="MFGKQIRMERIVDRNSGRVVVVPLDHGISMGPIPGLIDMRDTIN
                     KISLGGATAVLMHKGMVPYGHRTSGKDIGLIVHLSAGTGLGGDPNSKVIVTTVEEA"
     gene            495..1253
                     /locus_tag="O0X37_03430"
     CDS             495..1253
                     /locus_tag="O0X37_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62009.1"
                     /translation="MKRTLLLAAAAVLCIICAAAAGCITITLPSDTNPSTYVPTPAGP
                     GPVTSVVPGEWEGVLINADSTLVKYKIDCKFGGSAELEIDKRVGVKDQERTYYGTWTE
                     TGAGEYSLVFEAAGTYTFVMNNDGTGTLTTPEAATATLYPDHYSAVTPDPFIGDWDGR
                     VIKDSGVQIEYNIDIQRSGTAEIEIETTVPRVSETDVTYYGTWTQTAENVYEITGSIP
                     GLVETHTYTMTLAENGTATLSTPDNGDVLLIRDY"
     gene            1316..2734
                     /locus_tag="O0X37_03435"
     CDS             1316..2734
                     /locus_tag="O0X37_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HJK62010.1"
                     /translation="MKSSPFLAPVPFYWCDHCHVPVMGKLCACGRKTRQVSVTPPGDV
                     RPTFERDRTLINRLFEEQFGVPLIPEGHLALLNKVPDEDRMEEIILGGAVVCAIRYIP
                     AEKRWEVLPREAAALIVQPTKRIIVVTDEAGEYIKEGKSVLMPGVVSVSPEISVGDSV
                     FVMAENGECVAVGRAKMSYEETKEATRGQLVRTRRTQKPVVDTAPSTWEDAIRANNDI
                     LDLYEGKSIEFIRDVIAKNPDLQPTISYSGGKDSLVTLLITLKAVGKLPVIFADTGLE
                     FPETIENVQKVKETYGLELIERSGNAGFWEGFEVNGPPAVDFRWCCKACKLLPVKELI
                     EETWGEALSLIGQRKFESAKRMQSPRVWRNRNVPCQLSAAPIQHWTAMHDWLYLFREK
                     APYNPLYELGLDRIGCFMCPSSDISCMKDIAAMYPEMWAEWEKKLADWGSRNGKTPEW
                     AAKGLWRIRESGEEDGDHDSHF"
     gene            2718..3329
                     /gene="hisH"
                     /locus_tag="O0X37_03440"
     CDS             2718..3329
                     /gene="hisH"
                     /locus_tag="O0X37_03440"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HJK62011.1"
                     /translation="MTATSKIAVIDYGLGNLRSVVRGLEAAGCCPVITADPELIASAD
                     GIVLPGVGAFAEGMEKLAPVRAVVEEAVREKPLLGICLGMQMLLERSEEHGMHDGLGM
                     IPGTVRKFPRVPAMKVPQMGWNTITPSGHPLFADIADGTFVYFVHSYYADTTPEATIA
                     STEYIVPYASAVACGNALGVQFHPEKSGPAGLAILQNFVAMTE"
     gene            complement(3403..4158)
                     /gene="cobA"
                     /locus_tag="O0X37_03445"
     CDS             complement(3403..4158)
                     /gene="cobA"
                     /locus_tag="O0X37_03445"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HJK62012.1"
                     /translation="MSGKVYLVGSGPGGLGLLTAKAREVIDAADVILYDQLPGEEILA
                     TLPERAEKIDVGKYGGCHTMKQADIEALLVEKAKACDIVVRLKGGDPFMFGRGGEEME
                     TLREHGIAVEVVPGVTSGIAVPECVGIPVTHRSWASQVTFVTGHEDPEKEVSSIDWKW
                     LAGSPGTIVIFMGVKNLPIIANLLIENGKAPETKIAVIERGFRKDQRVTCATLADIGE
                     VAARVGVKPPAIIVIGEVVSLYRDGSEGAWAHQ"
     gene            complement(4155..>4387)
                     /locus_tag="O0X37_03450"
     CDS             complement(4155..>4387)
                     /locus_tag="O0X37_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HJK62013.1"
                     /translation="ERAVMELVGGGCFTPQGIFCREGHLIAEVLSLDGTRSERIAEKV
                     QSIEEATEIGAQFREIASELIEEAKTALGLRS"
BASE COUNT         1081 a         1137 c         1207 g          962 t
ORIGIN      
        1 gcctcctcaa cggtggtgac aataactttg gagttgggat ccccgccaag accggttcct
       61 gcggagaggt ggacgatcaa tccgatgtct ttaccggagg tacggtggcc gtaggggacc
      121 atccctttgt gcataagaac agcagttgct ccacctagag agatcttgtt gatcgtgtcg
      181 cgcatatcga tgagaccagg aatcggcccc atggagatac cgtgatccag agggacgaca
      241 acaactctgc cggagttgcg gtctacaatt ctttccatac ggatttgttt tccgaacatg
      301 tgtgctacat gttagcacgg atgacacttc aatgtttttg tttggttagg tctggatgga
      361 gaacaaaaac agacagccat ctatctctgg cgcatatcgg ggattgacaa cggcagcaga
      421 cgtccgctcc accagagaca aaaatacaga aacatacagt gaacataata gcaggtaaca
      481 caaaaagtag aggtatgaag cgaacactct tactcgctgc cgccgcagta ctctgtatca
      541 tctgcgcagc tgctgcagga tgtatcacca ttactcttcc gtctgacaca aacccgtcca
      601 cgtatgttcc cacaccggca ggaccagggc cggtaacaag tgtggttccc ggtgagtggg
      661 aaggtgtgct gattaacgct gacagtacac tggttaagta caagatcgac tgtaaatttg
      721 gtggatcggc agagcttgag atcgacaaac gcgttggcgt gaaggatcag gagcgaacct
      781 actacggtac gtggacggag acgggagccg gagagtacag tctcgtattt gaggcagccg
      841 gaacatacac gttcgtgatg aacaacgacg gaacaggaac actgacaacg ccggaggctg
      901 caaccgcgac actgtatccg gatcactaca gtgcagtgac gcctgatccc tttatcggag
      961 actgggacgg cagagtcatc aaagacagcg gagtccagat agagtacaat atcgacatcc
     1021 agcgttccgg aaccgcagag atagaaattg agacaaccgt tccacgggtc tccgagacgg
     1081 atgtaaccta ctatggaaca tggacacaga ccgcagagaa tgtctatgag atcaccggct
     1141 caattcctgg tctggttgag acacacacct acaccatgac cctggcggaa aacggcactg
     1201 caacactatc aacacctgac aacggtgacg ttctcctcat ccgcgactac taatccaagg
     1261 cggaacctga acggttccgc tgtgttcatt atttttcagt acccattagt tatccatgaa
     1321 gtcctccccg tttcttgcac cagtaccgtt ttactggtgc gaccactgcc atgttccggt
     1381 aatgggcaag ctgtgtgcct gcggcaggaa aacacgccag gtatccgtga caccgccggg
     1441 agatgtacgg cccacatttg aacgggacag aaccctgatc aaccgtctgt ttgaggagca
     1501 gttcggtgtc ccgctgatcc cggagggaca tcttgctctt ctgaacaagg tccctgatga
     1561 agacagaatg gaggagatca ttctcggagg agcggtggtg tgtgcaatcc gttacattcc
     1621 tgccgagaaa cgctgggagg ttctgccccg tgaggccgct gcacttattg tccagccgac
     1681 gaaacggatt atcgttgtta ctgacgaggc gggagaatac atcaaagagg gaaagagtgt
     1741 cctcatgccc ggtgttgtgt cagtatcgcc ggagatttct gtgggtgaca gcgtctttgt
     1801 gatggcagaa aacggagagt gtgttgcggt cgggcgggca aagatgtcat atgaggagac
     1861 aaaagaggcg acccgcggcc agctggtgcg tacgcgcaga acacagaaac cggtggtgga
     1921 cacagccccg tcaacctggg aggacgcaat ccgggcaaac aatgacatcc tggatctgta
     1981 tgagggaaag tccattgagt tcatccgtga tgtcattgca aaaaatccgg atctgcagcc
     2041 gacgatctcc tactcgggag gaaaagacag tctggtcaca ctgctgatta cgctgaaggc
     2101 ggttggaaaa ctgccggtta tatttgcgga taccgggctt gagtttcccg aaacgatcga
     2161 aaatgtacag aaagttaagg agacgtatgg tctcgagctg attgaacgtt ccggcaatgc
     2221 agggttctgg gaaggctttg aggtgaacgg gccgcctgcg gtggactttc gctggtgctg
     2281 taaagcatgc aagcttctgc cggtaaaaga actgattgag gagacatggg gtgaggctct
     2341 gtcactgatt ggacagcgga agtttgagtc agcaaaacgg atgcagagcc ccagagtgtg
     2401 gcggaaccgg aatgttccct gccagttgtc ggcggcaccg attcagcact ggactgccat
     2461 gcacgactgg ctgtatctgt tccgcgaaaa agcaccatac aacccgctgt atgagttagg
     2521 tctcgatcgt atcggctgct ttatgtgtcc gtcatccgat atctcctgta tgaaagatat
     2581 tgcggcgatg tacccggaga tgtgggcaga gtgggagaag aagcttgcag actggggaag
     2641 ccggaacggc aaaacaccgg agtgggccgc aaaaggtctg tggcgtatca gagagtcagg
     2701 cgaggaggat ggtgatcatg acagccactt ctaagattgc agtgatcgat tatggactcg
     2761 gaaacctccg gagtgtggtc cgtggactgg aagctgcggg atgctgcccg gtaatcacag
     2821 cggatccgga gctgattgca tcagcggacg gcattgtcct gcctggagtc ggagcatttg
     2881 ctgagggtat ggagaagctt gcaccggtcc gggcggttgt ggaggaggca gtacgggaaa
     2941 aaccgctgct tggtatctgt cttggaatgc agatgctcct tgaacgaagt gaagagcatg
     3001 gaatgcacga cggccttgga atgatccccg gaaccgtgcg aaagttcccc cgtgttccgg
     3061 ctatgaaagt tccccagatg ggatggaaca ccatcacccc gtcgggtcat ccgctgtttg
     3121 cagatattgc ggacgggacg tttgtgtatt ttgtacactc ctactatgcg gatacaacac
     3181 cagaagcgac gattgcgtca actgagtata ttgtgccgta cgcatccgcg gtggcatgcg
     3241 gaaatgcact aggcgtacag ttccatccgg aaaagagcgg gcctgcggga cttgcaattc
     3301 tccagaactt tgtggcaatg accgagtgaa agattccggc cctgaaaaaa gaaaagcaca
     3361 tccagcgtgc tattcccaaa aaaaagtgta tggaaacgta ggtcactgat gtgcccatgc
     3421 cccttccgat ccgtcacggt acaggcttac gacctcgccg attacgatga ttgccggggg
     3481 cttaacacca acacgggccg caacttcgcc aatgtctgca agcgttgcac aggtgacccg
     3541 ctgatccttg cggaacccgc gttcaatcac cgcaattttt gtctctggcg cttttccgtt
     3601 ctcgatcaga aggtttgcaa tgatcgggag attttttaca cccataaaga tgacgatcgt
     3661 tccgggactt cctgcaagcc acttccagtc aatggaggaa acttcctttt cgggatcctc
     3721 gtgaccggta acaaatgtca cctgacttgc ccaggaacgg tgtgtcaccg gaataccgac
     3781 acactcggga acggcaatgc cgcttgtcac accgggcacc acctcaacgg caatgccgtg
     3841 ttcgcggagc gtttccatct cctcgccgcc gcggccaaac ataaacggat caccgccctt
     3901 taggcggaca acaatatcac aggccttcgc cttttcaaca agcagtgcct caatgtccgc
     3961 ctgtttcatc gtgtgacacc cgccgtactt gccgacatcg atcttttccg cacgttccgg
     4021 aagtgttgct aggatctctt ctcccggcag ctgatcgtac aggattacat cagctgcatc
     4081 aatcacctca cgtgcctttg ctgtcagcag gcccagtcct cccggtccgg accccactaa
     4141 atacaccttt ccactcatga tcgtaacccg agagcagttt ttgcttcctc gattaactcg
     4201 gaggcaatct cgcggaactg tgctccgatc tccgttgcct cctcaatact ctgaaccttc
     4261 tctgcaatcc gttcactgcg ggtaccgtcc aaggacagaa cttctgcaat gagatgacct
     4321 tcacgacaga aaataccctg cggcgtgaaa catcctccgc caaccagttc cattacagca
     4381 cgctcaa
//
LOCUS       DALUAJ010000162         1930 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_29730,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000162 DALUAJ010000000
VERSION     DALUAJ010000162.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29730"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="O0X37_03455"
     CDS             <1..258
                     /locus_tag="O0X37_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HJK62014.1"
                     /translation="ICLAAYQKIVEHFRPKGEELRLYSNEYRYSDVAFARDVEAEDVS
                     RLLAGMPVQVIDTGFAIHLQPLGLSKGAALAKLAELMGLTP"
     gene            277..429
                     /locus_tag="O0X37_03460"
                     /pseudo
     CDS             277..429
                     /locus_tag="O0X37_03460"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
     gene            complement(485..736)
                     /locus_tag="O0X37_03465"
     CDS             complement(485..736)
                     /locus_tag="O0X37_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HJK62015.1"
                     /translation="MKSEQFTHSIRRKRVMSTDGKIIGQIKNITFDSATGQLLNLIVR
                     PDQTFDASGYKLDGDAITLPFEAVKDITDFIVVDRYMLR"
     gene            complement(798..1676)
                     /locus_tag="O0X37_03470"
     CDS             complement(798..1676)
                     /locus_tag="O0X37_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HJK62016.1"
                     /translation="MDLSLIQKDILITLISLYHQYSHPIKGEDIADIIKRNPGTVRNQ
                     MQALKALGLVDGVPGPKGGYHPTSRAYSELNVTADDSDSEVAILRNGESVDGVRVNEI
                     DFTTLTHADLCHALIKVIGNVRVFEVGDKVTIGPTPVNKLLIKGEVFGKDEINSALLI
                     STSEMTSLPKLPIREYMTTPLVSLEADMTVAYVMKTLLQRRIHGAPVIEGSRLLGIVT
                     QTDIVSAIDRGVPMDGQIGDIMVRNVVTIPGDMRLYEVIRRFKEQDIGRVIVTEEGKP
                     VGILTHSDIIRVFPTL"
     gene            1730..>1930
                     /locus_tag="O0X37_03475"
     CDS             1730..>1930
                     /locus_tag="O0X37_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HJK62017.1"
                     /translation="MYFDNIPTIPTADELLDRSFRRAAKKMREKNNKNRANEDFVRAI
                     TQATHDKLVSIIQSFPEFEQLPP"
BASE COUNT          519 a          546 c          443 g          422 t
ORIGIN      
        1 atctgccttg ccgcctacca aaagatcgtg gaacacttcc ggccgaaagg agaagaactc
       61 aggctttaca gtaacgaata ccggtactcc gacgtcgcat ttgcccgtga cgtggaagca
      121 gaagacgtga gcagactcct tgcgggaatg ccggttcagg tcatcgacac aggctttgcc
      181 atccacctcc agcccctggg actctccaaa ggagcagcac ttgcaaaact tgcagaactc
      241 atgggcctta ccccgtagga cttccttgcc gcaggcgact cgatcaacga cgtcagcatg
      301 cttaatgcct ccggagtagc cattggtccg gcaaacgcaa gccccgaagc acgtgcagca
      361 gcagaccaca tcatggacaa acccttcgga gaaggaacag ccgaagctct tgcccaatac
      421 tttccctgaa aaaaagattc tgaagagtat tcccctgccc cggcaggaga acctgtaact
      481 atttttaccg cagcatatag cggtcaacca cgataaagtc cgtaatatcc ttgaccgcct
      541 caaacggcag agtgattgca tcgccgtcaa gcttgtagcc cgacgcatca aacgtctgat
      601 ccggacgcac gatcagattc agcagctgcc cggttgcact gtcaaaagtg atgttcttga
      661 tctgtccgat aatctttccg tcggtgctca tcactctctt tctgcggata gaatgggtaa
      721 actgctcact cttcatagta atcagtagaa tctattgata acaaaagtat atatccgttt
      781 tgaataaaac agacggttca aagggtggga aacacccgga taatatcaga atgggtaaga
      841 ataccaaccg gcttaccctc ctcggtcaca atcactctcc cgatatcctg ctccttgaac
      901 ctgcggataa cctcatacag ccgcatatcc ccggggatgg taacaacatt acggaccata
      961 atatccccaa tctgcccgtc cattggaaca ccgcgatcga tcgcggaaac aatatccgtc
     1021 tgcgtaacaa tccccaaaag ccgcgaccct tcaatcaccg gcgcaccgtg aatcctccgc
     1081 tgcaaaagcg tcttcatcac ataggcaacc gtcatatctg cctcaagaga aacaagcggg
     1141 gttgtcatgt actcgcggat cggaagcttc ggcagagatg tcatctccga cgttgagatg
     1201 agaagagcac tgtttatctc atcttttccg aacacctccc ctttgataag cagcttgttc
     1261 accggtgtgg gaccgatggt cactttgtcg ccaacctcaa aaacccggac attgccgatc
     1321 accttgatca gagcatggca gagatcggcg tgtgtcaggg tggtaaaatc gatctcattc
     1381 acgcgcacgc cgtccacaga ttccccgttg cggagaattg caacctcgct gtccgaatca
     1441 tccgcagtaa cattgagttc gctgtaggcg cgggaggtgg ggtggtatcc tcctttcggc
     1501 cccggaaccc cgtcaaccag acccagcgct ttgagggcct gcatctgatt gcgaaccgta
     1561 ccggggttgc gcttgataat atctgcgata tcttcccctt ttatcggatg cgaatattga
     1621 tgataaagcg agataagtgt aataaggata tctttttgga tcagcgacaa atccatactg
     1681 attgttcaga ctgccaccct aataaagaaa tcgagtgaat caggcaacta tgtactttga
     1741 caatattccc acaataccca ccgccgacga actgctggac cgcagtttcc ggcgtgccgc
     1801 aaaaaagatg cgggaaaaaa ataataaaaa ccgcgcaaac gaggacttcg tacgggcaat
     1861 cacccaggca acccatgaca agctggtaag catcatccag agcttcccgg agtttgaaca
     1921 gctaccgccg
//
LOCUS       DALUAJ010000163         1888 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_29849,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000163 DALUAJ010000000
VERSION     DALUAJ010000163.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29849"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(264..809)
                     /gene="fae"
                     /locus_tag="O0X37_03480"
     CDS             complement(264..809)
                     /gene="fae"
                     /locus_tag="O0X37_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016840 - carbon-nitrogen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-activating enzyme"
                     /protein_id="HJK62018.1"
                     /translation="MGFLQSVDNAVGGVISPFTRVMFGEALVGEGAEVAHIDLVIGPK
                     GSAVEQAFVSALASPQKGHTPLLAVVTPNLPPKPATLMVNKVTIKNANQAVMMFGPAQ
                     HAVAMAVMDSVEEGVIPKEKAEDLLIIASVFIEWDAADKKKVHDWNYEATKLAIKRAI
                     TGEPKVDEVLAKKNSAVHPFN"
     gene            1136..>1888
                     /locus_tag="O0X37_03485"
     CDS             1136..>1888
                     /locus_tag="O0X37_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HJK62019.1"
                     /translation="MLKAVKIKNGVYWVGAIDWNIRDYHGYTLPGTTYNAYLVIGDKV
                     ALIDGTYPGHEWQMIERIESVLPLEKIDYIVANHIEIDHSGSLPLLSKKLPNVPIYMT
                     EIAKKGFARHYDTSAWNIHTIKNLENLPLGGGKTLTFLEAPFLHWPDSMFTYLGEDKI
                     LFPNDAFGQHVASSERFDDELGIDVAMEHAAKFVANLIVPLSPKVLKKLGEVTELGVP
                     IEMIAPSHGIIWRSHAADIINAYVNWANGVSKN"
BASE COUNT          529 a          457 c          425 g          477 t
ORIGIN      
        1 ccataatccg ggaaaaggag acctcaccct catcacggat aagaaacaga atattattcc
       61 gaaccggact actcaccgct gtaacagtac cgtcactgga aaacaacttc aagggaggga
      121 ctagatcagg ttcaggcatg atatgttcac gtgtttttgc atctattttt tcaaaaaggt
      181 tagattggat attaatttgc ggcgaacaga taagaatgtt cgcgatggtg caaaaaagtt
      241 tgacagaatc tctgaaaaaa agattagttg aagggatgaa ctgctgagtt tttctttgcc
      301 aggacctcat ccactttcgg ttcaccggta atggcacgct tgatggcaag ctttgttgcc
      361 tcatagttcc agtcatgtac tttcttcttg tctgctgcat cccactcaat gaaaacggat
      421 gcaatgatca gcagatcttc agctttctct ttgggaatga caccctcttc aacagagtcc
      481 ataacagcca ttgcgactgc atgctgggca ggaccaaaca tcatcactgc ctgatttgca
      541 ttctttatgg taactttgtt aaccataagt gtcgccggtt ttggcggcag attgggagta
      601 accacggcaa gaagcggcgt gtgccctttc tgcggagagg caagcgcact cacgaaggcc
      661 tgttcgacgg cacttccttt tggtccgatc acaagatcga tgtgggcaac ttctgcgccc
      721 tcaccaacca gtgcttctcc aaacattacg cgggtaaacg gagagatgac tccgcccacc
      781 gcattgtcaa cgctttgtaa aaatcccatg ttggtacctc gcgggctaat ttcagatcca
      841 tttcgggtct gctgatctgt tgatatggcg ataacgggat aaatatttgc tctttgggat
      901 acaaaaatat ttgacaatac tgttttttga gagttccaca atcacatgag tttaccgagt
      961 aaatttacca caaaaatggc atttacttat gaaaaacaga aaatcaaata aacgaaatat
     1021 ctcaacggaa aaagtgccgc gaccgaacat gtattaaata tcaagaacaa atatgtgagc
     1081 aaccatagga cactgcggct ctgtatcctg ctgcagtcca ggatgggaaa ttaccatgtt
     1141 aaaagcagta aaaattaaga atggcgtcta ctgggtagga gctatcgact ggaacattcg
     1201 tgactatcac ggctacacac ttccgggaac cacctacaat gcatacctcg tcattggcga
     1261 caaggttgca ctgattgacg gtacctatcc aggacatgag tggcagatga ttgagcgtat
     1321 tgaatcggtt cttccactgg agaagatcga ctacatcgtt gcaaaccaca ttgagattga
     1381 ccactccggt tccctcccgc ttctctccaa gaaactcccg aatgtcccga tctacatgac
     1441 cgagatcgcc aagaaaggat ttgcacgcca ctatgataca agcgcatgga acatccacac
     1501 gatcaagaac ctggagaacc tgccccttgg cggtggaaag acgctgacct tccttgaggc
     1561 accgttcctc cactggccgg attccatgtt cacgtacctt ggagaagaca agattctctt
     1621 cccgaacgac gcattcggtc agcatgttgc atccagcgag cgtttcgatg atgaactcgg
     1681 aattgatgtc gccatggagc acgcagcaaa gtttgttgca aacctgatcg ttccgctttc
     1741 cccgaaggtt ctcaagaaac tcggcgaagt tactgagctt ggtgtcccaa ttgagatgat
     1801 cgcaccgtca cacggtatca tctggagaag ccatgctgca gacatcatta atgcctacgt
     1861 caactgggca aatggtgttt ccaagaac
//
LOCUS       DALUAJ010000164         1654 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_29885,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000164 DALUAJ010000000
VERSION     DALUAJ010000164.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29885"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(155..>1654)
                     /locus_tag="O0X37_03490"
     CDS             complement(155..>1654)
                     /locus_tag="O0X37_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HJK62020.1"
                     /translation="VFSVEIFPAIDEERFRVLYSQNASRPNTPVNIIVGALIIKELFA
                     LSDDEIVENLMLDIHYQYALHTTSYEEQPLSDKSLSRFRKRCYEYEKTTGVDLFHECV
                     CDLNRKIAKLMGINSHVKRMDSLMIEANIRKLSRIELLYSCVARLAKRIEKKEGIGKL
                     TGLEHYCQPGDRNFIVYHCRNTDMNERIETILKDADQLLLRSAAQYQETEEYRLLVRC
                     LDEQTIAGSGGRRLRKKGEESPSTTSLQNPTDPDATYCVKAGKDHIGYAANVVESVDS
                     SGSVITDYQYEKNIFSDPRFLRDHLTRTEEQKETVRIVADGGYVGQECRELAAEKNIE
                     LINTALKGVAVPDIYGGFLLSDNGKSVQQCPAGYAPIRCSYTERTSRIRAYFKSEHCM
                     GCDHREECQAKQRRATCQMSLSRSAVERARQQQQMHAPDFQNWHRIRNGIESVPSILR
                     NRYQVDKMPVRGFIPSKFFFGAKISAMNFNKLLRHRRGLLFCAQNPLLS"
BASE COUNT          395 a          418 c          375 g          466 t
ORIGIN      
        1 gtaccacatt ttgacattaa ggtcaaaagg gaggttttga agttagcgca gctactgtta
       61 ggcctgtttt gctgctccaa aacaatatca taccaataaa aattgcctgc cgtccctcta
      121 tacgggacgg caggcaatcc aatcttccga ttgtttaact gagtagagga ttttgggcgc
      181 agaacaacaa gcctctccgg tgtcgaagaa gtttgttgaa attcattgcg gagattttag
      241 ccccaaaaaa gaatttactc gggataaatc ctctcacggg catcttgtct acttggtacc
      301 ggttacgcag tatggacgga accgattcta ttccgttccg aattctatgc caattttgaa
      361 aatcaggtgc atgcatctgt tgctgctgcc ttgcgcgctc cacagccgag cgggaaagtg
      421 acatttgaca ggtcgcccgt cgctgctttg cttggcattc ttcgcggtga tcacatccca
      481 tgcaatgttc agatttaaag taggccctga ttcgggacgt tctttctgtg taggagcagc
      541 gtattggcgc atatccggct ggacattgct gcacactctt tccgttatca cttagcagaa
      601 aaccgccgta aatatccggt acagctacac cctttaatgc tgtattgatg agctcaatat
      661 ttttctctgc tgcaagttct ctgcactcct gccccacata gccgccatcc gcaacaatcc
      721 gcacggtctc cttttgttct tctgtgcgcg taagatggtc gcgaagaaag cgcgggtcgg
      781 aaaatatgtt tttctcatat tgataatccg taatcacgga accgctgctg tccacactct
      841 ccaccacatt cgcagcatac ccaatatggt ctttccctgc ctttacacag tatgtcgcat
      901 ccgggtcagt aggattttgc agggaagttg ttgacgggga ttcctctccc ttcttgcgaa
      961 gccgtcttcc gccgcttccg gcaatggtct gctcgtccag gcatcggaca agcagacgat
     1021 actcctctgt ttcctgatat tgtgccgcac tccgcagcag aagctggtcc gcatctttca
     1081 gaatggtctc tattcgctca ttcatatctg tgttccggca atgatacacg atgaaattgc
     1141 ggtcacctgg ctggcagtaa tgttccagcc ctgtcagttt accgatgcct tccttctttt
     1201 cgatacgctt ggcaagtctt gcaacacagg aatagagcag ctcgatccgg cttaattttc
     1261 ggatatttgc ctcgatcatc agcgaatcca tacgtttgac gtgagaattg atccccatca
     1321 gttttgcgat tttgcggttc agatcgcaga cgcactcatg gaacaaatct acgccggtag
     1381 ttttttcgta ttcatagcag cgtttccgaa aacgggacaa ggatttgtcg ctcaacggct
     1441 gctcctcgta gctggtggtg tggagagcat actgataatg gatgtccagc atcagattct
     1501 caacaatctc gtcatcggac aaagcaaaca gttcttttat aatgagcgca cccacaataa
     1561 tattgactgg ggtattgggt cgtgaagcat tttgagaata caaaactcgg aaacgttctt
     1621 cgtcaatggc gggaaaaatc tcaacagaaa acac
//
LOCUS       DALUAJ010000165         8592 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_30016,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000165 DALUAJ010000000
VERSION     DALUAJ010000165.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30016"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="O0X37_03495"
     CDS             <1..1102
                     /locus_tag="O0X37_03495"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HJK62021.1"
                     /translation="YFLTSNLVGGQFGMGIWFATIAGLVAGFGIGLITEYYTSFDYKP
                     TKAISESCQTGAATNIINGFAKGMESTVWPVLIIAAATYVAYYFAGMYGIGIAAVGML
                     STLGLTLAVDAYGPVADNAGGIAEMSGQPHSVREVTDTLDAVGNTTAAIGKGFAIGSA
                     ALTALALFSAYATAVGLEAIDLLNTNVFIGILIGGMLPFLFSALTMTAVGQAAQKIVI
                     EVRRQFKEIKGLMEGTAEPDYTSAIAIATDSAIHKMILPGVIAIVAPIIVGLVLGPLA
                     LGGLLVGSLVAGVLLALTMANSGGAWDNAKKYIEIGNYGGKGSDAHKAAVVGDTVGDP
                     FKDTSGPALNILIKLMSMIALVFVPVILAFAA"
     gene            complement(1283..1615)
                     /locus_tag="O0X37_03500"
     CDS             complement(1283..1615)
                     /locus_tag="O0X37_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HJK62022.1"
                     /translation="MNYDPKSLIGTPVDLASLTEYQPGSVVSRMVINKKAGTVTAFAF
                     EAGEGLSEHTAPYDALVIVLEGEADIPVGGVLHHLKAGQMIIMPANVPHAVHPTTRFK
                     MMLVMIHE"
     gene            complement(1660..2142)
                     /gene="rimI"
                     /locus_tag="O0X37_03505"
     CDS             complement(1660..2142)
                     /gene="rimI"
                     /locus_tag="O0X37_03505"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HJK62023.1"
                     /translation="MVVVNGVGGTAAGCTLRKATLEDIPEIYRIELMCFSDPWDYKGM
                     IEMMGVFLTSFYVAEADGRIVGFSAGAVEETDEEKYGHICNIAVVPELRGFGIGRLLL
                     RKLERDFFLDGCCACSLEVRVSNTAAQGFYKKIGYEEVIVFGEYYADGEDAIVMMRWF
                     "
     gene            2193..3452
                     /locus_tag="O0X37_03510"
     CDS             2193..3452
                     /locus_tag="O0X37_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HJK62024.1"
                     /translation="MVTIYPFVGLHPSDEAFAFVPSVPYDVISAEEAAAEIAKNDKTL
                     LRVIRTDAELPDIDPHDDRIYERAREMFAKMKEDGLLVSDEKPAFYIYRITLGEETFT
                     GLVSCVSVAEYKDDTIKKHELTRYDKEEDRTRHIDAVSAHTGQVFLLYKDPGNIHAYI
                     SGLADAMQPFGEYRSEGGNLHQIYRVTDHADIAKLETMFAEVPNLYIADGHHRAKSSV
                     NVYDRRKAEGRCTPDAERFMSVLFAHDKVRIYGYHRLVRDLAGMNADQFLSDLALRFT
                     ITPIKKVDITKNTVPPLADTDGSTHIIHLYLESQWYELTRPVDPKADAIARLDVSVLQ
                     ESVLDDMLGIIDPRGDPRISFVGGIVPLSDVTREVDAGEFSAAFIMQPVTAQGVIDVA
                     DNAMIMPPKSTWFEPKLLSGMVIHEIH"
     gene            complement(3505..4392)
                     /locus_tag="O0X37_03515"
     CDS             complement(3505..4392)
                     /locus_tag="O0X37_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62025.1"
                     /translation="MTEETDRAADITPEKTNPPKRRRVTKKKTETETVQAPTETPEKQ
                     TENKIELAAIEATTETEEKATEEAERPHKAVPEQFLNDLAQFADILEKTPALLDPIRE
                     VFEYHLVDPIDRKTFPRMVNAMSMLLGPSATMVVMTACHVNSAAFFEDLLYAIKDEKS
                     RGAVWIIQHLTALYGNRVQQAYTLSSGTMDEDWHTVDVNTYKREGENAYWIVDVKMML
                     YSGKESQIKMTPDSIFQLVEILAAELGRNIPIENVDPGLVKKCEENFTIFYEKFYGAK
                     KCEETEKTDGEEEHPPGYA"
     gene            complement(4514..5386)
                     /gene="frhB"
                     /locus_tag="O0X37_03520"
     CDS             complement(4514..5386)
                     /gene="frhB"
                     /locus_tag="O0X37_03520"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit beta"
                     /protein_id="HJK62026.1"
                     /translation="MEYLGQYKAVYKAKAGCEDILSKAQDGGIVTAMFAYALETGIID
                     GAIVAGPGEEAYKPEPMIATTVDELLAARGTKYSISPNMSLIKEATRSYGLDKIGIVG
                     TPCQIQAVRKAQLYPIGLRDVPDKIALALGIFCMENFPYQGLYQMVEDHCATKIDNVT
                     KMDIGKGKFSVYTERGVNAQIPLKVTHKYEQPGCHVCLDYVANLADISTGSVGTPDGW
                     STVFVRSAAGEAAFNKAVEAGWIIAESMDGVKPGLELVTKLATEKITKNQKYIEHRAH
                     ADHPALKPLRNPYI"
     gene            complement(5407..6393)
                     /gene="frhG"
                     /locus_tag="O0X37_03525"
     CDS             complement(5407..6393)
                     /gene="frhG"
                     /locus_tag="O0X37_03525"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit gamma"
                     /protein_id="HJK62027.1"
                     /translation="MGLLDKFKSLLFGKGDVSEGTQDEKPVASKQEPVASKQEEPVSA
                     KIEAPVTKAEPADMKVEQKPIIQKPKEEKPVADKITVGHVHMSGCTGCLVSLADNYEG
                     LLTILDKYADLVYCLTLADVRHIPEMDVALVEGSVCLNDECSMEEILETREKAKIVVA
                     LGGCACYGNTTRFCRGGQQNQPQHEAFLPVGDLIKVDVYIPACAPTPQQIRNVCLMAY
                     LLLKGNDEQKALATAYLTPLMNLATAGTEACGCDLMTEVINQGLCCGCGSCAAACTVR
                     AITMQYGRPQIERDLCIKCGACYAQCPRSFFSFDVCGQYEAITNLIGEVMKP"
     gene            complement(6396..6878)
                     /gene="frhD"
                     /locus_tag="O0X37_03530"
     CDS             complement(6396..6878)
                     /gene="frhD"
                     /locus_tag="O0X37_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-reducing hydrogenase, FrhD
                     protein"
                     /protein_id="HJK62028.1"
                     /translation="MEGLFPEIIIAGVGNELFGDDGFGPAVIRALSEYELPDNVKAMD
                     VGLGGPHLVFSMLDPEETKKLIIVDCMDFGGKPGDLTKIPADLLPEGRQERYMDAHSG
                     NLLDPIGRLKGKMDIVILGCQPAYIPAPDSEDFALELSPEVQGAIPKTVNVILKEIGS
                     "
     gene            complement(6887..8254)
                     /gene="frhA"
                     /locus_tag="O0X37_03535"
     CDS             complement(6887..8254)
                     /gene="frhA"
                     /locus_tag="O0X37_03535"
                     /EC_number="1.12.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase subunit alpha"
                     /protein_id="HJK62029.1"
                     /translation="MSKVVEISPTTRHEGHSKLVLKVNDEGIIERGDWLSITPVRGVE
                     KLAIGKTYEQVPKIASRVCGICPIAHTLAATEAMEASMGVEIPDDAYLLRIILQCANR
                     LHSHALHDILALPDMYLPGTDSKINPFSPEEPVRSVAKRIQTLRMIGQTIGEIAGGEA
                     VHPSNPRVGGMYKNITPRARTKIYDLAKQALPLAREQMEFMIAVFENYAKRDWAEMCG
                     REVAIPKDLGFHQQGYMATDPCYGSSSLDEHPTWDPARWLEVRPWDWYMGEEEITLED
                     PTYPIGGTTKAGTKAWPQMQACTGVPLYDGQPVEVGPRARLAMYRNYDRKGAIGLNIA
                     REMEYMDSVYKIIEAADALNTSGKVVADVIPQGDGTIGWAANEAPRGADVHLAKVRNG
                     KVEWFSMLVPTTWNFPTCSRALTGAPWELAEVVVRAYDPCVSCATHMIVLDEDNRLVA
                     QKLLE"
BASE COUNT         1888 a         2221 c         2089 g         2394 t
ORIGIN      
        1 ctacttcctg acctcgaacc ttgtcggcgg ccagtttggt atgggtatct ggtttgcaac
       61 catcgccggt cttgttgcag gctttggtat cggtctgatc accgagtact acacctcctt
      121 cgattacaaa ccgacgaagg caatctccga gtcctgtcag accggagctg cgaccaacat
      181 catcaatggt tttgcaaagg gaatggagtc gaccgtctgg ccggttctga tcattgcagc
      241 cgcaacctat gttgcatact acttcgctgg catgtacggt atcggtattg cagctgttgg
      301 tatgctgtct accctcggtc tgacccttgc agttgatgca tacggtcctg ttgctgacaa
      361 tgccggcggt atcgccgaga tgtccggtca gccgcactct gtccgtgagg ttaccgacac
      421 gcttgatgca gtcggaaaca cgactgctgc aatcggtaaa ggatttgcaa tcggttccgc
      481 tgcactgacc gctcttgctc tcttcagcgc ctacgcaact gcagtgggtc ttgaggcaat
      541 cgatctctta aacaccaatg tgttcattgg tatcctgatt ggcggtatgc ttccgttcct
      601 cttctctgca ctcaccatga ccgcagtcgg tcaggcagca cagaagattg tgattgaggt
      661 tcgccgtcag ttcaaagaga ttaaaggcct gatggaaggt actgcagagc ctgactacac
      721 gtctgcaatt gctattgcaa ccgactcggc aattcacaag atgattcttc cgggcgttat
      781 tgcaatcgtt gcaccgatca ttgtcggtct tgttctcggt ccccttgcac ttggcggtct
      841 ccttgtcggt tcccttgttg ctggtgtgct tcttgcactt accatggcaa actccggagg
      901 agcttgggac aatgcaaaga agtacattga gattggcaac tacggtggta aaggctctga
      961 tgcccacaag gcagcagttg tcggtgacac cgttggtgat ccgttcaagg atacatctgg
     1021 tccggctctg aacattctga ttaagctgat gagcatgatt gccctggtgt ttgtgccggt
     1081 aatcttagct ttcgctgcat aatcagagaa aaatcatatc cacatttttt tccaagactg
     1141 ttttggtact ccttaatagt atgtaccagt cgtattcaaa aaagtgtgat gatggttttt
     1201 ctggcaaaat aaaccaggtg ttgttgaatc tttttccggt ttgtgtactg tttttgcaac
     1261 aacaaaccac taatggcttc ttttattcgt gaatcattac cagcatcatc ttgaaccggg
     1321 ttgtcgggtg caccgcatgt ggtacatttg ccggcataat aatcatctgt ccggctttca
     1381 ggtgatgtaa aacaccgccg accgggatat ctgcctctcc ttcaagaaca attaccagcg
     1441 cgtcatatgg tgcggtatgt tcggaaagcc cttcccctgc ttcaaatgca aatgcagtaa
     1501 ccgttcccgc ttttttattg atgaccatcc gggacacaac cgatcctggc tgatactccg
     1561 tgagagaagc aagatcaaca ggggttccga tcagggattt tggatcatag ttcataccga
     1621 gtatctcgct gtctgaaaag ataaacctct gttttccggt caaaaccacc gcatcatcac
     1681 gattgcatcc tcaccatccg cgtagtactc gccgaacaca atcacctctt catacccgat
     1741 ctttttgtaa aatccctgtg cagccgtgtt acttacccgg acctcaagac tgcatgcaca
     1801 gcatccgtcc agaaaaaagt cgcgttccag tttgcggagc aagagtcttc caatcccaaa
     1861 cccccgcagt tccggaacta ccgcaatgtt acagatatgg ccgtacttct cctcatccgt
     1921 ctcctcaacc gctcccgcgg aaaacccgac gatccgtccg tccgcctctg caacatagaa
     1981 agaggtcaga aacactccca tcatctcaat cataccctta tagtcccatg ggtctgaaaa
     2041 gcacatcagc tcgatccggt agatctccgg aatatcctca agtgtcgcct tacggagagt
     2101 acagccggcg gcagttccgc cgacaccgtt caccactacc attgttattt tagtattgtc
     2161 accgaactat attataggag tctttgaaac caatggtcac catctacccg tttgtcggtc
     2221 ttcacccgtc ggatgaggca tttgcttttg ttccgtccgt tccgtatgat gtcatcagcg
     2281 cagaagaagc agccgctgaa attgcaaaga atgacaaaac ccttcttcgt gttatcagaa
     2341 cagatgcaga actgccggat attgatccgc atgacgatcg tatctatgag cgtgcccgtg
     2401 agatgtttgc aaagatgaaa gaggacggac ttcttgtctc agatgaaaaa ccggccttct
     2461 acatttaccg catcactctt ggagaggaaa cgttcactgg tcttgtttcc tgcgtgagtg
     2521 ttgccgagta caaagatgat acgatcaaaa agcatgaact gacccgctac gacaaagaag
     2581 aggatcgtac ccgtcacatc gatgccgtct cagcacacac cggtcaggtg ttcctgctct
     2641 acaaagatcc gggaaatatt catgcctaca tctcgggtct tgcggatgcc atgcagccgt
     2701 ttggtgagta ccggagtgag ggcgggaacc tgcaccagat ctaccgcgtg acagatcacg
     2761 ctgatatcgc aaaacttgag acaatgtttg ccgaggtacc caacctctac atcgcagacg
     2821 gccatcaccg tgccaagtca tctgtcaatg tgtacgatcg caggaaggct gagggaagat
     2881 gcacgccgga tgccgaacgg ttcatgagcg ttttgtttgc tcatgacaaa gtgcggatct
     2941 acgggtacca ccggctggtt cgtgatcttg caggcatgaa tgctgatcag ttcctcagcg
     3001 atcttgccct gcggtttacg ataacaccaa ttaagaaggt tgacatcacg aaaaatacag
     3061 ttccgcccct tgcagatact gacggttcaa ctcatatcat ccatctctat ctggagagcc
     3121 agtggtatga gttgacacgt ccggttgatc cgaaagcgga tgcgattgcg cggctggatg
     3181 tttcagtgtt gcaggaatct gtactcgatg atatgctcgg catcattgat ccccgcgggg
     3241 acccccgtat ctcctttgtc ggcggtattg ttcccctgtc ggacgttacc agggaggttg
     3301 atgccggtga gttctcagct gcattcatta tgcagccggt gaccgctcag ggagttatcg
     3361 atgttgcaga caatgcgatg attatgccgc cgaagtccac gtggtttgaa ccaaaacttc
     3421 tctccggcat ggtcatccat gaaatacact aacatttttt aatgggatag aattcccgct
     3481 gattagcgtc tcgtttgaaa aaaattatgc atatcctggg ggatgttcct cttccccgtc
     3541 agttttttcc gtctcttcac atttttttgc gccgtagaac ttttcataaa agattgtaaa
     3601 gttctcctcg cattttttca caagacccgg atccacattt tcgattggaa tgtttcttcc
     3661 aagctctgct gcaagaattt ccacgagctg gaagattgag tccggcgtca ttttgatctg
     3721 gctctctttc cccgagtaga gcatcatttt tacatcaaca atccagtacg cgttctctcc
     3781 ttcacgcttg taggtgttga catccaccgt atgccagtcc tcatccattg ttcctgatga
     3841 cagggtgtat gcctgctgta cacggtttcc atataatgca gtcaggtgct ggatgatcca
     3901 cacagcaccg cggcttttct catccttgat tgcatacagc agatcttcaa agaatgctgc
     3961 tgagtttaca tggcaggctg tcatcacaac cattgttgct gacggaccaa gcagcattga
     4021 cattgcattt accatgcggg ggaatgtttt gcgatcgatc ggatccacca aatgatactc
     4081 aaacacctcg cggattggat ccagcagtgc aggtgtcttt tccagaatat cggcaaactg
     4141 tgcaagatcg ttcaggaact gttcaggaac tgctttgtgc ggtctctccg cctcttctgt
     4201 cgctttttcc tctgtttcag tcgtggcctc aattgcggca agctcaattt tgttttcagt
     4261 ctgtttttct ggagtttctg tgggagcctg aaccgtttcc gtttcagttt ttttctttgt
     4321 aacccttcgt cgttttggtg ggtttgtttt ttccggggta atatctgctg ctcggtccgt
     4381 ctcctcagtc atactaactc atttggatcc gatatacaaa aacctcatcg gtctgcagaa
     4441 cttcagtcat cagattttca aaaaaaggtt gtacccgaga ggggtgggta catgtacaac
     4501 ccttaggaca cggttagatg tatgggttgc gcagtggttt cagtgccggg tggtccgcgt
     4561 gggcacggtg ctcgatgtac ttctggttct tggtgatctt ctcggttgcg agtttggtca
     4621 cgagttccag acctggtttg acaccgtcca tcgactcagc aatgatccag cctgcttcga
     4681 ctgccttgtt gaaggctgcc tctcctgctg cggagcggac gaagacagtg gaccatccat
     4741 ccggagttcc tacggaaccg gttgagatat ctgcaaggtt tgcgacgtag tcaagacata
     4801 cgtggcatcc tggctgctca tacttgtggg taaccttcag cggaatctgt gcgttcacac
     4861 cgcgctcggt gtacacagag aacttgccct ttccaatgtc catcttggtg acattgtcga
     4921 tcttggttgc acagtggtcc tcaaccatct ggtagagacc ctggtatggg aagttctcca
     4981 tacagaagat accaagagca agagcaatct tgtccggaac gtcacggaga ccgatcgggt
     5041 agagctgtgc ttttcttact gcctgaatct gacagggggt accgacgata ccgatcttgt
     5101 caagaccgta gctgcgggtt gcctccttga tcagggacat gttcggagag atcgagtatt
     5161 tcgttccacg tgctgcgaga agctcgtcaa cggtggttgc gatcattggc tcgggcttgt
     5221 atgcctcttc tcctggtcct gcgacgattg ctccgtcaat aatgcctgtc tcaagtgcgt
     5281 aggcaaacat tgcagtgacg attcctccgt cctgggcctt tgagagaatg tcttcacagc
     5341 cggctttggc tttgtagact gccttgtatt gtccaagata ttccattttt ttctcctccg
     5401 ggaccctcag ggtttcatga cctctccaat gaggttcgta atcgcttcat actgtccgca
     5461 cacgtcgaag ctgaagaagc ttctcgggca ctgtgcatag caggcgccgc acttgataca
     5521 cagatcgcgc tcgatctgcg gtctgccgta ctgcatcgtg attgcacgaa cagtacaggc
     5581 tgcagcacag ctgccgcatc cgcagcagag gccctggttg atgacttcag tcatcaggtc
     5641 gcatccgcat gcttcagttc ctgctgttgc aaggttcatc agcggggtca ggtatgcagt
     5701 tgcgagtgct ttctgctcgt cgttgccttt cagcagcagg tatgccatca ggcacacatt
     5761 gcggatctgc tgcggggttg gtgcacatgc tgggatgtac acatcgacct tgatcaggtc
     5821 accgacaggc aggaatgcct catgctgggg ctggttctgc tggccaccac ggcagaagcg
     5881 ggtggtgttg ccgtaacatg cacagcctcc gagagcaaca acaatctttg ctttctcacg
     5941 ggtttccaga atctcttcca ttgagcactc gtcgtttaaa cagacggaac cctcgacaag
     6001 tgcgacatcc atctctggga tgtggcgcac atcggcgagc gtcaggcagt agacgaggtc
     6061 agcgtatttg tcaaggatag taagtaatcc ttcgtagtta tctgcaaggg acacgagaca
     6121 tccggtacat ccgctcatgt gtacgtgtcc tacagtaatc ttgtcagcca ctggcttctc
     6181 ctcctttggt ttttgaataa ttggcttttg ttcgaccttc atgtcagccg gctctgcctt
     6241 cgtaacaggc gcttcaatct ttgctgacac aggttcttcc tgtttactgg caacaggctc
     6301 ctgtttactg gcaacaggct tttcgtcctg cgttccctcg ctgacatcgc cttttccgaa
     6361 cagtaagctt ttgaatttat ctaataatcc catctttagc ttcctatctc tttcaagatg
     6421 acgtttacag ttttgggaat ggccccttgt acttccggac tcagttcaag agcaaagtct
     6481 tcactgtccg gtgcaggtat ataggcgggc tggcatccga gaataacaat gtccattttc
     6541 cctttcagtc tgccgatagg atcgagcaga tttccggaat gggcatccat gtaacgctcc
     6601 tgtctgcctt ccggcaggag gtcggcgggg atcttcgtca ggtctccggg ctttccccca
     6661 aagtccatgc agtccacaat gatcagtttc ttcgtttctt ccggatcgag catggaaaaa
     6721 accagatgtg gacctccgag gcccacatcc attgctttta cattgtcggg aagttcgtac
     6781 tcggaaagtg ctctgatcac tgccggacca aatccatcgt cgccaaacag ctcgttaccg
     6841 acgccggcga ttattatttc tggaaacagt ccttccatgt atatactcac tcaaggagct
     6901 tctgtgccac aagccggttg tcttcatcca gtacaatcat gtgggttgca caggatacac
     6961 aggggtcata tgcacggaca acaacttctg caagctccca tggtgcgccg gtaagtgcac
     7021 ggctgcaggt cgggaagttc caggtcgtcg ggacgagcat ggagaaccac tcaactttgc
     7081 cgttgcggac ctttgcgagg tgaacatctg cgccacgcgg tgcttcgttt gctgcccatc
     7141 cgatggttcc gtcaccctgc ggaatgacgt ctgcgacgac ttttccggag gtgttgagtg
     7201 catctgctgc ctcaatgatc ttgtagacgg agtccatgta ttccatttca cgtgcaatgt
     7261 tgagaccaat ggctcccttg cggtcgtagt ttctgtacat tgcaagacgt gcacgtggtc
     7321 ctacctcaac aggctgtccg tcgtagagcg ggacaccagt acatgcctgc atctgcggcc
     7381 atgcctttgt acctgctttg gtggttccgc caattgggta tgtcgggtct tcaagagtga
     7441 tctcttcttc gcccatgtac cagtcccaag gtctgacttc aagccagcgt gctggatccc
     7501 aggtcgggtg ctcgtcaaga gagctgcttc catagcatgg gtcggttgcc atgtatccct
     7561 gctggtggaa accaagatcc ttcggaattg caacttcgcg accgcacatc tctgcccagt
     7621 cacgctttgc atagttctcg aagactgcaa tcatgaactc catctgctca cgtgcgagcg
     7681 ggagtgcctg ctttgcaaga tcatagatct tggttcttgc acgcggagtg atattcttgt
     7741 acataccacc aacacgcggg ttggacgggt gaactgcttc tccacctgcg atctcaccaa
     7801 tcgtctggcc gatcatacgc agagtctgga tacgctttgc aacagatctg accggctcct
     7861 caggggagaa cgggttgatc ttcgagtcag tgcccggcag gtacatatcc ggcagtgcaa
     7921 gaatgtcgtg cagtgcgtgg gagtgcagtc tgtttgcaca ctggagaatg atacgcagaa
     7981 ggtatgcgtc gtctgggatc tctacaccca ttgatgcttc cattgcctct gttgcggcaa
     8041 gagtgtgggc aatcggacag ataccacaca cacgggaagc gatcttggga acctgctcat
     8101 aggtcttacc gatggcaagc ttctctactc cacgaaccgg cgtgatggaa agccagtctc
     8161 cgcgctcaat gattccttca tcgtttactt ttaaaacgag cttggagtga ccttcatgtc
     8221 tggtggtcgg ggaaatttct actactttcg acaatgttat cgcctcatcc taaatatttt
     8281 aggttgttcg attagctata caggaaaatt accattgtcc ttttgggtta atgacacgta
     8341 ccgaagtacg ctatcttaac tttctcgcat ggcatttatt aaagattttc tctctttgtg
     8401 tcaaagaatt acctagctgt tttcggtcct ttttcaattg gttatactta gatcagatga
     8461 aattatcgta agtgttacta ttttttaaaa ttgtagcact catcttgaaa aaacgtttct
     8521 tttgccattg ttctttgaga gagataacgt atttttttgg cgctatctag aattatttat
     8581 gcgctctgga aa
//
LOCUS       DALUAJ010000166         1688 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_30049,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000166 DALUAJ010000000
VERSION     DALUAJ010000166.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30049"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /gene="pstB"
                     /locus_tag="O0X37_03540"
     CDS             <1..210
                     /gene="pstB"
                     /locus_tag="O0X37_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833488.1"
                     /note="ATP-binding protein; PstABCS is an ATP dependent
                     phosphate uptake system which is responsible for inorganic
                     phosphate uptake during phosphate starvation; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HJK62030.1"
                     /translation="TSKIETLITELKKNYTVIIVTHNMQQAARVSDTTGFMYLGKLVE
                     VDDTKKIFSNPSEELTERYVTGRFG"
     gene            211..882
                     /locus_tag="O0X37_03545"
     CDS             211..882
                     /locus_tag="O0X37_03545"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HJK62031.1"
                     /translation="MNEYFHLELGSYKDQVNWYGRFALGMLKNSLMAFETVDREIAAD
                     VVRQKDYIASQYDLLNERGVLLIALNQPMATDLRIIACSLDMITSSERVGRYGKDIGE
                     LVVQFTDREHVAPIAKQLTGMGDLTSSMLEIVYNSFAAGETDPLSSLSDMEESVDGMY
                     DVIYDDCVAAMEQDVSVVPQCSAYHMINRYLERCADHACRMGEKVYYMQTGKRVSIDG
                     ISRMG"
     gene            complement(927..>1688)
                     /locus_tag="O0X37_03550"
     CDS             complement(927..>1688)
                     /locus_tag="O0X37_03550"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HJK62032.1"
                     /translation="TILRENPADHRLYLTTDLTDPQWHCEITHAEGTPLTTVTSSSKY
                     YTINGFILSYSHEILVTITLTGTVPADTIGKELTLLSAEESTSGDIVISSYEKKTTIG
                     SAAVTPTPTQTITVTPTETQTAVPTATKTPVPTVTPTPKPTPTVATLIASSSPSGANL
                     FIDNNYKGFTPITQTDITPGTHVVTLKKEGYLSDEQKITFEAGTTYEISFTLTEEPKA
                     SEVFTDLIKQYPTMALIGIILIGFGVLALLIRRKH"
BASE COUNT          348 a          301 c          533 g          506 t
ORIGIN      
        1 acgtccaaga ttgagacgct gattacggag ctgaagaaaa attacaccgt tattattgtt
       61 acccacaata tgcagcaggc cgcacgtgtc agtgatacga caggatttat gtatcttggc
      121 aagcttgttg aggtggacga tacgaaaaag atcttttcga atccttccga ggaacttacc
      181 gaacgttatg ttaccgggag gtttggttaa atgaacgagt attttcatct ggaacttggt
      241 tcctataagg atcaggtgaa ctggtacggc cggtttgcct taggtatgct gaagaactcc
      301 ctaatggcgt ttgagacggt tgaccgtgag attgcggccg atgttgtccg gcagaaagat
      361 tatattgcaa gccagtatga tctgctgaat gaacgcggtg ttctgctgat tgcgctgaac
      421 cagccgatgg cgacggatct ccgcattatt gcatgcagtc tggatatgat tacgtcctct
      481 gagcgtgtgg gtcgctatgg taaggatatc ggcgagcttg ttgtgcagtt tacggaccgc
      541 gagcatgttg ctccgattgc aaagcagctg accggaatgg gggatctgac cagttccatg
      601 ctggagattg tgtacaacag ctttgcagcg ggggagactg atccgctttc ttcactttct
      661 gatatggagg agtcggttga cgggatgtat gatgttatct atgacgactg tgttgcggcg
      721 atggaacagg atgtttcggt tgttccgcag tgcagtgcgt atcatatgat caaccggtat
      781 ctggaacgtt gtgctgatca cgcctgccgg atgggggaga aggtgtatta tatgcagacc
      841 ggaaagcgtg tgtcaattga tggtatcagc cggatgggct gattcctttt tgattttttt
      901 ggtgctgcct tttgaaaaag agggggttag tgttttcttc tgatgagaag ggctaaaacc
      961 ccgaatccga tgagaattat gccaatgagg gccattgtcg gatactgttt gatcaggtcg
     1021 gtgaaaactt cggatgcttt tggttcctct gtaagggtga agctgatttc gtaggttgta
     1081 ccggcttcga atgtaatctt ctgttcatca ctgaggtacc cttctttttt gagggtgact
     1141 acatgggttc ccggggtgat gtcggtctgg gttatcgggg tgaatccttt gtagttgttg
     1201 tcaataaaga ggttggcacc ggacgggctg gaagaggcga tgagtgttgc aacagtgggg
     1261 gttggtttcg gcgtgggtgt tacagttggt accggggttt ttgttgcggt cggtacggct
     1321 gtctgggttt cagtgggcgt tacggttatg gtctgtgtgg gtgttggagt tacagctgcg
     1381 gaaccgattg tggttttctt ttcgtacgag gagatgacga tgtcgccgct ggtactttct
     1441 tctgcgctga ggagggtgag ttcttttcca attgtatctg cggggactgt tcctgtcagt
     1501 gtgatggtta cgaggatttc atgggagtag gagaggatga atccgtttat ggtgtagtat
     1561 tttgaggagc ttgttactgt ggtaagggga gttccttctg catgggtaat ctcacagtgc
     1621 cactgcgggt cggtaaggtc ggtggtgaga tagaggcggt gatctgccgg attttcccgc
     1681 aggatagt
//
LOCUS       DALUAJ010000167         1828 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_30777,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000167 DALUAJ010000000
VERSION     DALUAJ010000167.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30777"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="O0X37_03555"
     CDS             complement(<1..300)
                     /locus_tag="O0X37_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HJK62033.1"
                     /translation="MGQGKFAARKLVRSAKKFRWSDSVYARRALKLKLKADPLEGAPL
                     GRAIVLEKVGVEAKQPNSAIRKCVRVQLIKNGRQVTAFAVGDGAINFIDEYDEVTI"
     gene            complement(506..1660)
                     /locus_tag="O0X37_03560"
     CDS             complement(506..1660)
                     /locus_tag="O0X37_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62034.1"
                     /translation="MNLPAYIYDESRICGNIQKLRNTFPQFTILYSIKANPYPPLVRY
                     IASQNIGADAASKNEVLLACKEGFTPDQIYYSGPGKTKTDLVETVGRCNLIADSFHEL
                     QLINQIAEEKEIIQEVGIRINPNFTMNGRPGVPGKFGIDEELCTKDAFAAYPAVRITG
                     MHTHVKSQELNWEKIAGYYENVFSLAVRVQQEIGHPLTYINFGSGIGVPYLKTETPVN
                     LTELRKHTAELVAFYQPKIAAKLLIESGRYLVCEAGTYITEVLDRKQSRGKTYLIVAG
                     GLNGFVRPVLSAFAEDGHAEAEPLFTAKDAWRITSSATATETEVVDIVGNLCTSSDIL
                     AENIRLPRANIGDRITFTHAGSYACTLSPHQFGSQKKPDEVLRPQGTISH"
     gene            1725..>1828
                     /locus_tag="O0X37_03565"
     CDS             1725..>1828
                     /locus_tag="O0X37_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HJK62035.1"
                     /translation="MTTRIYKETHFDASHRLMHYEGKCSRLHGHRWEV"
BASE COUNT          399 a          402 c          462 g          565 t
ORIGIN      
        1 gatggtgact tcgtcgtact catcgatgaa gttgattgca ccgtcgccga ctgcgaatgc
       61 agtgacctga cggccgtttt tgatcagctg aacacgaacg cactttctga ttgcggagtt
      121 cggctgtttt gcttcaacac caaccttctc taagacgatt gcgcgtccga gcggtgcgcc
      181 ctcaagcggg tctgccttca gcttcagttt gagcgctctg cgcgcgtaaa ctgagtcgct
      241 ccaccggaat ttcttggcgg aacggacaag ctttcttgct gcgaattttc cctgtcccat
      301 taaattacct cagttacagt agattttttg gatagatgaa tcttgtaaca cagtctcctg
      361 tgctgtgttt cccggaaaaa aacgtgcacg tagatttacg ggaaactatt cctctgttgg
      421 aatattgtga atgcgagggc agggtcttga gagatagcct tcccttgcat gttcctatac
      481 ttatgagcag gctgtcctaa taatattaat gggagattgt gccctggggc cggagtactt
      541 cgtccggttt tttctggctg ccgaattgat gcggggagag tgtacaggca taactgccgg
      601 catgtgtaaa ggtgatccgg tctccaatat tggcgcgtgg cagccggatg ttttctgcaa
      661 gaatgtctga ggaggtacag aggtttccga caatatcaac aacctctgtt tctgttgcgg
      721 ttgcactcga ggtaattctc catgcatctt ttgcagtgaa gagcggttct gcttctgcgt
      781 gcccgtcctc tgcaaatgcg gagagtaccg gacggacaaa tccattaagt ccccctgcga
      841 cgatgaggta tgtttttccg cgggattgtt ttctgtccag tacttccgtg atataagtac
      901 ctgcttcaca gacgagatat cttcccgatt cgatcagaag ttttgcggct atctttggtt
      961 ggtagaatgc gacgagttct gctgtatgtt ttctgagttc cgtaagattt actggggttt
     1021 ctgttttcag atagggtacg ccaattccgg atccaaaatt gatataggta agtggatgtc
     1081 cgatctcttg ttgcactctg actgccagtg agaaaacatt ttcataatat ccggcgattt
     1141 tttcccagtt caactcttgg gatttcacat gggtatgcat cccggtaatc ctgactgcag
     1201 gatatgcggc aaatgcatct tttgtacaga gttcttcatc gataccgaac tttcctggga
     1261 ctcctggtct gccgttcatg gtaaagttgg gatttatgcg gatgccgacc tcctgtataa
     1321 tttccttctc ttctgcaatc tgattgatga gttgaagttc atgaaagcta tcagcgatga
     1381 ggttacagcg tccaacggtt tcaacaagat cggttttggt tttccctgga cctgaataat
     1441 agatttgatc cggtgtgaat ccctctttgc atgcgagcag tacctcattt tttgatgctg
     1501 cgtctgcccc tatgttctga gatgcgatgt accgaacaag cggagggtat gggtttgctt
     1561 ttatggagta gagaattgta aactgtggaa aggtgtttct gagtttttgt atattgccac
     1621 agatgcggct ttcatcatat atgtatgcgg gaaggttcat cctgtttagt ctgttaccct
     1681 atcccctata attatagttt cccgcgagca atatgtggtc aagtatgact acccggatct
     1741 ataaggaaac acatttcgat gcgtctcatc ggctgatgca ttatgaggga aaatgttccc
     1801 ggctgcacgg tcaccgctgg gaggttga
//
LOCUS       DALUAJ010000168         2161 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_31186,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000168 DALUAJ010000000
VERSION     DALUAJ010000168.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31186"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(154..579)
                     /locus_tag="O0X37_03570"
     CDS             complement(154..579)
                     /locus_tag="O0X37_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthase"
                     /protein_id="HJK62036.1"
                     /translation="MRKIVATGTFDILHPGHLFYLEESRKLGDELWVIVARERNVVHK
                     PRPIVPEEQRLKMISGLRCVDHALLGDLEDMFRPIADIDPEVVTLGFNQKFSEEKLIA
                     QMRERGIRADVVRIGPFCGCAFNSSTRIIEEALKRRGSQ"
     gene            complement(576..950)
                     /locus_tag="O0X37_03575"
     CDS             complement(576..950)
                     /locus_tag="O0X37_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HJK62037.1"
                     /translation="MFVRAIDREVLDLLLEMGRSSSPDEFIVMLGSEKGVITTVYPIA
                     GTTVTTDSATIFMDMVPLGMQIAGTAHSHPNGVLHPSAADLQTFAETGQCHIIVGDPF
                     EADSWRCFDREGHEKSLEVVSS"
     gene            991..1728
                     /locus_tag="O0X37_03580"
     CDS             991..1728
                     /locus_tag="O0X37_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HJK62038.1"
                     /translation="MRSGKATCPGTVTCNGQEIPYTIIYSDRSRSWAIEVKADASVIV
                     RMPQNIPPEKAKTLVETKSEWIFQQVQKYTSRAQISRRYTDGEKLPFLGQDYPIIRKT
                     GDYAKAEFTDGLFYITLPGGFTESDQTALARDLIIMLYRRVGAAALEDIINHYSPLAG
                     VDPPKLRIRMQKRKWGCCTPKNGIIVNARILLAPKIVAEYIVVHELVHLRFRHHQKTF
                     WNEVERLMPTYRDAETILKNDGWKFVF"
     gene            complement(1785..2117)
                     /locus_tag="O0X37_03585"
     CDS             complement(1785..2117)
                     /locus_tag="O0X37_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HJK62039.1"
                     /translation="MKMVVAVIRPEKVDDVVDALEAVNVPGVTITDVRGRGEQGGICL
                     QYRAGKMQIHTLPKTKLEIVVPDKDVETIIKTIREHARTGKKGDGRIFVLPVEAAAWV
                     RTDDFITG"
BASE COUNT          582 a          599 c          439 g          541 t
ORIGIN      
        1 acctccggcc gaacccatta caaccaaaca aacacttcat tctcacccac tcccctgtca
       61 caatctgatc agcagagatc tttactgcat caccgccttt ttcacggata aaatgagtca
      121 gtctgtcaac ttcctcatcg atagagtatt ttttcattgt gaacctctcc gtttcaatgc
      181 ttcttcaata atccttgttg atgaattaaa ggcacacccg cagaacggcc cgatccgaac
      241 aacatccgcc cgaataccac gctcccgcat ctgggctatc agcttctcct ccgaaaactt
      301 ctgattaaac ccaagcgtga ctacctctgg atcaatatct gcaatcggcc tgaacatatc
      361 ctccagatct cctaaaagtg catgatccac acatctgaga ccggagatca ttttcagacg
      421 ctgttcctct gggacgatcg gtcgcggttt atggacgacg tttcgttccc gggcgacaat
      481 cacccacagc tcatcaccca gcttccttga ctcctcaaga taaaacaaat gtccgggatg
      541 cagaatatca aacgtcccgg ttgcaacgat ttttctcacg aagaaacaac ctccaaagac
      601 ttttcatgac cttcgcgatc gaagcagcgc cacgaatctg cctcaaacgg atcacccacg
      661 ataatatgac actgacctgt ctcggcgaat gtctgcagat ccgcagcaga cggatgcagt
      721 acaccgtttg gatgactgtg tgcagttcct gcaatctgca ttccaagcgg taccatatcc
      781 atgaagatag tggcactgtc ggtggtaacc gtcgttcccg caattggata caccgttgta
      841 attacacctt tttctgatcc gagcatcacg atgaactcat cgggagagct tgacctcccc
      901 atttcaagaa ggagatccag aacttcccga tcaattgcgc gaacgaacat attctattta
      961 tgttggcgtg acggagagat aatcattcac atgcggtccg gaaaagcaac ctgccctggg
     1021 acggtcacct gcaacgggca ggaaatcccg tataccatca tatacagtga ccgcagcaga
     1081 tcatgggcca tagaagttaa ggccgatgca tctgtcatag tccgcatgcc acagaatatt
     1141 cctcccgaaa aagccaaaac tcttgtggaa accaaaagtg aatggatatt ccaacaggta
     1201 caaaaataca catcgcgtgc ccaaatatcc cgcagataca ctgatggtga aaaactccct
     1261 ttccttgggc aggactatcc tattatccgt aagactggtg actatgcaaa agcagagttt
     1321 acagacggat tgttctacat caccctccca ggtggtttca ctgagtctga tcagactgct
     1381 cttgcacgtg atctgattat tatgctgtac cggagagttg gtgctgccgc ccttgaagat
     1441 attatcaatc actattcccc tcttgcagga gtggatcctc caaaacttcg catacggatg
     1501 cagaagcgaa aatgggggtg ctgtacacca aaaaatggca tcattgtcaa tgcccgcatc
     1561 ctgcttgctc caaagattgt tgctgagtat attgttgtcc acgaacttgt ccatctcaga
     1621 ttccgccacc accaaaaaac gttctggaat gaggttgaaa gacttatgcc gacataccgc
     1681 gacgcggaaa caattctcaa aaacgacgga tggaagtttg tattctaacg tctcaaaaat
     1741 tagaaaatag agatgggaga tactccctca tcgagctaac agcattatcc ggtgatgaag
     1801 tcatcggtcc gcacccatgc agccgcctca acaggcagca caaagatacg gccgtcccct
     1861 ttcttccccg tccgtgcatg ttcacgaata gtcttgatga tagtttcaac atctttgtct
     1921 gggaccacga tctccagctt tgttttgggc agggtatgaa tctgcatctt gcctgcacgg
     1981 tactgcagac agatacctcc ctgttcaccg cgtccgcgga catcggtaat ggtaactccc
     2041 ggaacattta ctgcttcaag ggcatcaacc acgtcatcta ccttttcggg tctgataact
     2101 gctacaacca ttttcatact gtatcatctc ctccatttag tttgtcaact gctcaccgtg
     2161 c
//
LOCUS       DALUAJ010000169         3839 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_31516,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000169 DALUAJ010000000
VERSION     DALUAJ010000169.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31516"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="O0X37_03590"
     CDS             <1..1098
                     /locus_tag="O0X37_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HJK62040.1"
                     /translation="PETHESPEIRDLLKGNFTLMLVQWILAYVQRKIGMNVGIEPGAE
                     MKEAIRLAEERGIRLVLIDRDIRITLARFWGGMKILEKIKLIWALMQSIFAADDEDDE
                     TAEGIDKVAIDELTNPDIIEMALEEFRKFSPNGAKALIDERDAYLAHGVIDLEKSPYE
                     RAVVVVGAGHVPGITKYRENPSTLPPMAELLAKPKHYPWGKIIGGLFVAMFAVIIISI
                     AFSGATDLLVWAIIYWVLLHALFAGVATLLVRGHPFSAAVAAALAWATSLNPFLAAGW
                     FAAIVEAKMRPPTAGDLKAISKAESLGEMFSIPLFRILVVAAVANVGSSLATVCFFVF
                     LTPLLGVDLDMMTQILMTGLSNLWQFLTGWI"
     gene            1184..1714
                     /locus_tag="O0X37_03595"
     CDS             1184..1714
                     /locus_tag="O0X37_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HJK62041.1"
                     /translation="MNVRNPGIANFGITVIQSRHSVRKFKDEEIPPEFIRKALECASK
                     APTGRNKQPWIFVVIKNKETLAKIAELAPNGKFIEGAAVGILVLGESDWKFTVEDCSA
                     ATENLLLALHAYGYGGCWIAGNQMPYADAVRELVHVPETYKLVSIIAAGVPDVGGITL
                     AEKIPFEKLIYEEKYQ"
     gene            1822..3105
                     /locus_tag="O0X37_03600"
     CDS             1822..3105
                     /locus_tag="O0X37_03600"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase"
                     /protein_id="HJK62042.1"
                     /translation="MVRPVLQVALDLTELSRAEKIAEEAVAGGADWIEIGTPLVKSEG
                     MESVRRMRDLFPTTTLVADLKTSDTGAVEVEMAAKAGANVVCVLAAADDAVIADALRG
                     AALYGVEIMADMMNVSDPAARAEELAALGVQIINAHVGIDQQMTGKDPLALLDRLGKL
                     PVKIAVAGGLNAETAAAAAARGADIVIVGATIIKAADVTAAAAAVRAALDSPKAAQEE
                     KKTLDDQIRELLQTVSAPNVTDALYRKGAMAGLIERHVPHKMIGRAVTVQTFGGDWSK
                     PVQAIDSCMPGDILVISNDKRTDVAPWGELATHSAMNRGVAGIVIDGAVRDWDDIQTL
                     DIPVFATAVQPNAGEPKGFGEIGAEISCCGQTVRPGDWLIGDQSGVVVIPRERAYEVA
                     RRAVEVWKTETRVREEIRRGGTLGSVTQLLRWEKK"
     gene            3105..>3839
                     /locus_tag="O0X37_03605"
     CDS             3105..>3839
                     /locus_tag="O0X37_03605"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJK62043.1"
                     /translation="MVKKPCVQNPSDPVLWCKEAAARIEAGDHKGAIYCYEESLKIRP
                     EVPDVWYNLGLLLEKIGQPTAALGCYTKSSEMFPGDYRFFAERARLLASAGKYLDAVV
                     AVSDALAINPHEPQLHANKAGYLIRAGDAKGAAAAADAALAIEPTNSAAFVNKAHALV
                     ALGENGEAESVLRKGKEAVPEDERIAKMLANLLVRTGKWEEALTIADEVLLVTPEDDE
                     VWSIKGAACAYLGRKDEAVAAFGKAMK"
BASE COUNT          911 a          954 c         1141 g          833 t
ORIGIN      
        1 ccggagaccc atgagtctcc agagatacgg gacctgctga aaggcaactt caccctcatg
       61 cttgtacagt ggattctggc ctatgtccag cgcaagatcg ggatgaacgt cgggattgaa
      121 ccgggcgccg agatgaaaga ggcaatcaga cttgccgaag agcgcggcat ccgactcgtt
      181 ctcatcgacc gtgatatccg gattacgctt gccaggttct ggggcgggat gaagattctg
      241 gaaaagatca aactcatctg ggcactgatg cagtctatat ttgccgcaga cgatgaggac
      301 gacgagacgg cggagggtat cgacaaggta gccattgatg aactgaccaa tccggacatt
      361 atcgagatgg cgctcgaaga gttccgtaag ttctcaccga acggggcaaa ggcactgatt
      421 gatgagcgtg acgcatatct cgcccacgga gtgatcgatc tggagaagag tccgtacgag
      481 cgggccgtgg tggtggtcgg cgcaggacac gtcccgggaa ttaccaagta ccgggagaac
      541 ccgtcaaccc ttccgccaat ggcagagctt ctggcaaaac cgaaacatta cccatgggga
      601 aagatcatcg gcgggctgtt cgttgccatg tttgcggtga tcatcatttc catcgcattt
      661 tccggggcaa ccgatctgct ggtatgggca atcatttact gggttctgct gcatgcactc
      721 tttgccgggg ttgcaacact tctggttcgc ggacatccct tctccgcggc agttgccgca
      781 gcgcttgcct gggcaacttc actgaatcca ttccttgcag ccggatggtt tgccgcaatt
      841 gttgaagcaa agatgcggcc gccaaccgca ggcgatctga aagctatctc aaaagcagaa
      901 tcacttggcg agatgttttc cattccgctg tttagaatat tggtcgttgc ggcggtggca
      961 aatgttggaa gttcgcttgc gacggtctgt ttctttgtgt tcctgacacc gctactcggc
     1021 gtggatctgg acatgatgac acagattctc atgactggcc tgtcaaatct ctggcagttt
     1081 ttgaccggct ggatctaatc ttttttgaac aaacacaccg atttcccaca acaggattat
     1141 ccattcagaa aggctcttta cttctcctgc ataaggagta catatgaatg taagaaatcc
     1201 cgggattgca aacttcggta tcaccgtaat tcagtcacgt cacagtgtcc gcaagtttaa
     1261 ggatgaggag atcccgccgg agtttatcag aaaggcgctg gagtgcgcat ccaaagcacc
     1321 aaccggaaga aacaagcagc cttggatctt tgttgtcatc aaaaacaagg agactcttgc
     1381 aaagattgca gagcttgcac cgaacggaaa gttcattgag ggtgcagcgg tgggaatcct
     1441 tgttctcggc gaatctgact ggaagttcac cgttgaggac tgttctgcgg caaccgaaaa
     1501 tctgcttctt gccctgcacg cctatggata cggcggatgc tggattgcag gaaaccagat
     1561 gccctacgcg gatgcggttc gtgagctggt acatgtccct gagacctaca aactcgtctc
     1621 cattattgca gcaggggtac ccgatgtcgg cggcatcaca cttgcggaga agattccgtt
     1681 tgagaagctg atttacgaag aaaaatatca gtaagatcag agtcacgttt cacgtgaccg
     1741 aacaatcctt tttttctgcc aaatacggtc tcatccagta catcaccaac cttatcccct
     1801 cgcttcacca aattagttga tatggtcaga ccggttttgc aggtcgcact ggaccttacg
     1861 gaactctccc gggcagagaa gatcgccgag gaagcggttg ccggcggtgc cgactggata
     1921 gagataggaa ctcctctggt gaagagcgaa ggtatggaaa gtgtccgccg gatgcgggat
     1981 ctctttccaa ctacaacgct ggtagccgat ctcaagacct ccgataccgg agcggttgaa
     2041 gtggagatgg cagcaaaggc cggagcgaac gttgtctgtg tgcttgccgc agcggatgat
     2101 gcggttattg ccgatgcact gcggggggcg gcactgtatg gtgttgagat tatggccgat
     2161 atgatgaatg tcagtgaccc ggctgcccgg gccgaggagc tggctgcact tggggtacag
     2221 atcatcaacg cccacgtcgg tatcgatcag cagatgaccg gaaaagatcc gcttgcactg
     2281 ctggacaggc ttggaaaact gccggtgaag atcgcggttg ccggaggcct caatgcagaa
     2341 accgctgcgg cagccgcagc acgcggtgcg gatattgtca tcgtcggagc tacgatcatc
     2401 aaagcagcgg acgtgaccgc cgcagccgca gccgtccgtg cagcactcga cagtcctaag
     2461 gcagcacagg aggagaagaa aactcttgac gatcagatac gcgaactgct tcagaccgta
     2521 tccgcgccaa acgtcaccga cgccctttac cgcaaaggag caatggcagg acttattgag
     2581 cgccatgttc cgcacaagat gatcggtcgt gccgtgaccg tgcagacctt cggcggtgac
     2641 tggtccaagc cggttcaggc gattgactcc tgtatgccag gggacattct ggtcatcagt
     2701 aatgacaaac gtactgatgt ggcaccgtgg ggagaactcg ccacgcattc tgcaatgaac
     2761 cgaggggttg cgggaatcgt cattgacggt gcggtccggg actgggatga tatccagaca
     2821 ctggacatcc ccgtatttgc aaccgctgtg cagccgaacg caggagagcc gaaagggttt
     2881 ggagagatcg gcgcagaaat ttcctgctgc ggtcagacgg ttcgtcccgg tgactggctg
     2941 attggcgatc agtcaggtgt tgtggttatt ccccgtgaac gtgcctacga ggttgcacgc
     3001 cgtgctgtag aggtgtggaa gacggagacg cgggtaaggg aagagatccg ccgcggcgga
     3061 acgctgggaa gtgtgacgca actgcttcgc tgggagaaaa aataatggta aaaaagccgt
     3121 gtgttcagaa tccttccgat ccggtgctct ggtgtaaaga ggctgctgca cgaatcgagg
     3181 caggggacca caaaggtgcc atctactgct acgaggagtc gctgaagatc cggccggagg
     3241 tgccggatgt ctggtacaat cttgggctgc tcttggaaaa gattggtcag ccaaccgcgg
     3301 cacttggctg ctacacaaag tcttcagaga tgtttccggg agattatcgt ttttttgcgg
     3361 agcgtgcacg actgctcgcc tctgccggaa agtatcttga tgcggtggtt gctgtaagtg
     3421 atgcactggc aatcaatccc catgagcccc agctccatgc aaacaaggct ggctacctga
     3481 tccgtgccgg ggatgcaaag ggtgcggctg ccgcagcaga tgcggctctt gcaattgaac
     3541 cgacaaactc cgcagcattt gtaaacaaag cacatgcact ggtggcgctg ggagagaacg
     3601 gcgaggctga atcagttctc cgcaaaggaa aagaggctgt tccggaggat gaacggatcg
     3661 caaagatgct ggcaaacctt cttgtgcgta ccggtaagtg ggaagaggca ctcaccatcg
     3721 cagacgaggt tctgctggtt acgccggagg acgatgaggt ctggagcatc aaaggtgcgg
     3781 cgtgtgcata tcttggaaga aaggatgagg cggtcgctgc attcgggaag gcaatgaag
//
LOCUS       DALUAJ010000170         2327 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_31766,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000170 DALUAJ010000000
VERSION     DALUAJ010000170.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31766"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="O0X37_03610"
     CDS             complement(<1..1085)
                     /locus_tag="O0X37_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HJK62044.1"
                     /translation="MNTNTNRIKQWQEQEALSRFQLIAPLLQEDLDDAKRLQLRKQIA
                     QDNDISVRSIYRYEKAYREGQFAGLKPAAREKRRSQKLPENFDLLLEQAIQLRKEVPE
                     RSVAQIICILELEGYAAPGVLKRSTLERHLYRAGYGREQMQMYRDARGSSSKRFCKPH
                     RMMLVQGDIKYGPKLPIGKNGARVQTYLSSAIDDHSRLLLASRFYDSQEEAIVEDTFR
                     QAILRYGKFDACYFDNGSQYIARQIRLSLSRLGIRVLHAKPRSGKSKGKIEKFHQVVD
                     MFNREAKLKNIKTLEELNRLWAVFLDAYYHQKPHEGISEYYESLGAAVPESGITPLQE
                     WNRDSRPLTYLDVSVVSEAFLHHETRKV"
     gene            complement(1160..1648)
                     /locus_tag="O0X37_03615"
     CDS             complement(1160..1648)
                     /locus_tag="O0X37_03615"
                     /inference="COORDINATES: protein motif:HMM:NF040103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6431 domain-containing protein"
                     /protein_id="HJK62045.1"
                     /translation="MSSDDTPVCPVCGGTLKYRDTRLRIRKKEGGVKEYLMIRRLRCT
                     ECLRHHNELPDCLVPHKHYEAEVISGVLDGIVTSEDADSEDSPSLLTMLRWLQWFQMN
                     LANIEGFLRNAGYRILGLGEELLFSHASLLDTIRQIHQDCLERILRIIYNSGGFLPAV
                     PW"
     gene            2053..>2327
                     /locus_tag="O0X37_03620"
     CDS             2053..>2327
                     /locus_tag="O0X37_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62046.1"
                     /translation="MVDYREILRLQSLGYNITQIAGSLRSSRNTVREVERLADEKGIR
                     WPLGEKVTNPQLYVLLYPERQKKANVYLEPDCAWIHRELAKKGVNLTL"
BASE COUNT          586 a          562 c          577 g          602 t
ORIGIN      
        1 actttacgtg tctcatggtg cagaaatgct tcggatacaa cagagacatc cagataggtc
       61 aggggacggc tgtcacggtt ccattcctga aggggagtga tccctgattc cgggactgcc
      121 gcaccaaggc tttcgtagta ttcgctgatc ccttcatggg gcttttgatg atagtacgca
      181 tcaaggaaca cggcccacag ccggttcagc tcttccaggg ttttgatgtt ttttagtttt
      241 gcctcccggt taaacatatc cactacctga tggaactttt caatcttccc tttgcttttg
      301 ccgctccgcg gttttgcatg gagaacccgg atccccagtc ttgaaagaga gagcctgatc
      361 tgtctggcga tatactggga accgttatca aagtagcagg catcaaactt tccgtatcgg
      421 aggatcgcct ggcggaacgt atcctcaaca atggcttcct cctggctgtc atagaaacgg
      481 gaagccaaca gtaggcggga atggtcatcg attgcagagg aaagatacgt ctgtactctg
      541 gcaccatttt tcccaatcgg aagtttcggg ccgtacttga tatccccctg taccagcatc
      601 atacggtgcg gtttgcagaa acgtttggaa gaacttcccc gtgcatcgcg gtacatctgc
      661 atctgttccc ttccgtaacc agcccggtac aggtgccgtt ccagagtgga gcgtttcagg
      721 actcccggtg cagcatagcc ttccagttcc aggatgcaga tgatctgtgc gacagaccgc
      781 tccgggactt ctttacggag ctggattgcc tgctcaagca gaagatcaaa gttttccgga
      841 agtttctgtg aacggcgttt ttcgcgtgcg gctggtttta agccggcaaa ctgtccttca
      901 cggtaggcct tttcatagcg gtagatggac cggacggaaa tatcattgtc ctgagcaatc
      961 tgtttccgga gctgcagcct ctttgcatcg tccagatctt cctgaagcag tggtgcaatc
     1021 agctggaacc gggaaagggc ttcctgttcc tgccattgtt tgattctgtt tgtattggta
     1081 ttcatatata gtgacctcct tctgagacca ctatacctgc tggcccggtg acagacgaat
     1141 caaaacaggt gcaatagcat caccatggta cagcaggtaa aaaaccgccg ctgttgtaga
     1201 taatacggag gatacgttcc agacagtcct gatgtatttg gcgtatggta tcgagcagtg
     1261 acgcatgaga gaaaagaagt tcttctccca atccaagtat ccggtatccg gcattgcgca
     1321 ggaatccttc aatatttgcg aggttcatct ggaaccactg gagccatcgt aacatggtca
     1381 gcagggaagg agagtcttcc gaatcagcat cctccgatgt tacgattccg tcgagcacgc
     1441 cggagatcac ctcggcttca taatgcttgt ggggcacaag acaatcagga agctcgttgt
     1501 gatgacgcag acattccgtg cagcgaagcc tgcggatcat aagatactct ttcacgccgc
     1561 cttccttctt ccggatacgt agtctggtat cgcgatactt caaagtaccg ccacagacgg
     1621 ggcagacagg ggtgtcatca ctgctcataa caaaaaacac cggagtccgc cttgtaggtc
     1681 agtgtgtatt ctgatataat aaccatggtt tttataaccg ggtctcagag tacacgactt
     1741 tccagggagg gtgctctgag actttttctt tctgtagtct tatgacagta tacagagcaa
     1801 tccgctgaca ggcaagtaga tcataaatat gctattttaa gaaatcaata acatttaagt
     1861 tcattgcttt ttagtatcta taatataaat tgtatatttc agataaactg accatccttc
     1921 acggtcaatc tgaccacctt tcacggagat tcagatcact gatctcggag agggatagtc
     1981 ggcgatcaaa gtcattctta taatgatgat ggcacacatt cgtgtgacta tcatttaagg
     2041 aggacgatga tcatggtaga ttatcgcgaa atcctgaggc tccagagcct tgggtacaac
     2101 atcacacaga tcgccggatc gctgcgcagc tcccgcaaca ccgtaagaga agtggaacgg
     2161 cttgcagatg agaaaggaat ccggtggccg cttggagaga aagtaacaaa tccacagctt
     2221 tatgttctgc tttatccgga acgacagaaa aaagcaaatg tttatctgga accggactgt
     2281 gcctggatcc accgcgaact ggcgaaaaaa ggagtgaacc tgacact
//
LOCUS       DALUAJ010000171         1966 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_31807,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000171 DALUAJ010000000
VERSION     DALUAJ010000171.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31807"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="O0X37_03625"
     CDS             <1..257
                     /locus_tag="O0X37_03625"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075761.1"
                     /note="catalyzes the reversible transfer of the terminal
                     phosphate of ATP to form a long chain polyphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HJK62047.1"
                     /translation="SLTHRPIINALYEASNEGVKIDLIVRGICMLRPGVPNLSENIRV
                     ISIVGRFLEHSRVFYFRNNGDEQVFIGSPDMMSRNLKRRV"
     gene            440..1039
                     /locus_tag="O0X37_03630"
     CDS             440..1039
                     /locus_tag="O0X37_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HJK62048.1"
                     /translation="MVDLNKQELQTIHDAAVVLTEDFHHAEQVCRLSLAIYDDLSSAH
                     GYGKRTRRLLAAAALLHDIGWSISTVSHNKCGMTFIIEDRMLPLSLYERILVALAVRY
                     HRGGTPKRRHNLYGNLSKHDRQKVCLIAGIIRIADGLDRSHRSVVRSVHAKLSDETLV
                     LLCTGYGRGGPERRASARKADLLMKALSAKIQICWKRCP"
     gene            complement(1046..1624)
                     /locus_tag="O0X37_03635"
     CDS             complement(1046..1624)
                     /locus_tag="O0X37_03635"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HJK62049.1"
                     /translation="MADTTRKIADVADVLGISEDECRRIADEYETVLPCKKIGKVRVY
                     DENMVDRFRKIADLRAQGLPQDVILEAIKGGKSLEERAREDMRRMGVEVPKEPQMQVP
                     KPVPRTETEEELILAVRSAEAAVQTMDHRMAAMRERIAEDNAAVLDAVAEVAKEVAAL
                     RSEVHTLWDQIASLEQYFRNQDAQKKSGFWKR"
     gene            complement(1707..>1966)
                     /locus_tag="O0X37_03640"
     CDS             complement(1707..>1966)
                     /locus_tag="O0X37_03640"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HJK62050.1"
                     /translation="IGVVALLSGVTDVISAFSRGMSAGQRVLMLILGIIGIIVGMFFL
                     LFPEEGAPILTLVLGILLAIAGVVSLIQGYVYKKEYPHLVE"
BASE COUNT          531 a          512 c          434 g          489 t
ORIGIN      
        1 actccttaac tcacagaccg attatcaacg cattatatga ggcatcgaac gaaggagtaa
       61 agatagatct gatcgtccgt ggtatctgta tgcttcgccc aggtgtccca aacctttccg
      121 aaaacatccg agtcatctca atcgtcggca ggttcctgga acacagcaga gtgttttatt
      181 tcagaaacaa tggagacgag caggtattca ttggtagccc agatatgatg tcacgtaacc
      241 tcaagcggcg tgtttagatc atctgtccgg ttctggatcg caaaatcaag aatgcaatca
      301 tcaatagaat tctccctgcc ttcatccacg acacagcaaa aggacatctg cttacatcag
      361 atggtatata tctccctccc aagcatcccg aatctaatct gggagcacaa cagatgttta
      421 tcacaatgag aaaggtctgg tggtagatct gaacaaacag gagttacaga ctatccatga
      481 tgcagcggtt gtactcaccg aggatttcca tcacgcagaa caggtctgtc gtctctctct
      541 tgcaatctat gatgatctct catccgctca tggatacggc aagaggacgc gaagacttct
      601 tgcagcagcg gctcttctgc atgatatcgg ctggagtatc tcaactgtct ctcataacaa
      661 gtgtgggatg acattcatta tcgaggatcg gatgctgccg ttatcactgt atgaacggat
      721 tcttgtggca cttgccgtcc gttatcacag aggagggaca cccaaacgtc gtcacaatct
      781 ctacggcaat ctctccaaac atgaccggca aaaagtgtgt ctgattgcag gtatcatacg
      841 gatagcagac gggctggatc gatcccaccg cagtgttgtc cgatcagttc acgcaaaact
      901 atcagatgaa accctcgtcc tcctctgtac cggatacgga cgaggaggac ctgagagacg
      961 agcttcagca agaaaagcgg atctgctaat gaaggcatta tcagcaaaga ttcagatctg
     1021 ctggaaacga tgcccataac ctgaattacc ttttccagaa tccggacttc ttttgtgcat
     1081 cctgatttct gaagtactgt tccagtgatg caatctgatc ccagagtgtg tgtacctcac
     1141 tgcgaagtgc tgcaacttct tttgcaacct cagcaactgc atccaaaact gctgcattat
     1201 cctcagcaat tcgctcccgc attgctgcca tccgatgatc catcgtctga acagctgcct
     1261 ctgccgatct gaccgcaagg atgagctcct cctcggtttc tgtccggggg accggctttg
     1321 gaacctgcat ctgcggttct ttcggtacct caacccccat gcgcctcatg tcctcccgtg
     1381 cccgttcttc aaggcttttc ccgcctttga ttgcttcaag tatcacatcc tgcggtaggc
     1441 cctgcgcacg aagatcggcg atctttctaa accgatcaac catgttttca tcgtacaccc
     1501 ggaccttgcc gatcttttta caggggagaa cggtttcata ctcatcagct attcgccggc
     1561 attcatcctc ggatatccca agaacgtcag cgacatccgc aatttttcgt gtggtatcag
     1621 ccatagatat ttctacgttt ttcatgggac aaagactgat aaaaggtttg ggatgaggaa
     1681 cccaaaaaaa tgagagagtt gtatccttat tcaacaagat ggggatactc ttttttgtag
     1741 acatagccct gaatcaggga tacaacacct gcaatagcaa gaaggatacc aagaaccagg
     1801 gtaagaatcg gagcaccttc ctccgggaag agaaggaaga acataccaac gataataccg
     1861 ataattccaa gaatcagcat cagtacccgc tgacctgcag acattccgcg gctgaaggca
     1921 gaaataacat cagttacccc gctgagaagg gcgactacac caataa
//
LOCUS       DALUAJ010000172         4539 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_31879,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000172 DALUAJ010000000
VERSION     DALUAJ010000172.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31879"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(361..1668)
                     /locus_tag="O0X37_03645"
     CDS             complement(361..1668)
                     /locus_tag="O0X37_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62051.1"
                     /translation="MKLWKSALIITACLMLVLIAAPVSAAQVSYSTAGIELFATAEGG
                     NELVPGTETDFTIKLTNNAQYEVVAAESGLQRQYDVATAYQLRVTLEDADGITVKTGT
                     QTVSQIAPGESATLTYRVSVDQYATPGPQTLKVRVVYNELFSVENYGDALGYYWLNDR
                     VADVPLDVTVRSQVSPEVLEVSCDDISVGTTGFLTLTIKNAGGMDAQNAYASLITSSS
                     GISSTAIVPVESSIFIGDFPAGSTKTVTFKVSVANNAEVNEYPVGLQIVYQDNYGVDQ
                     VSSPVIVGVPVDGKVEFKVTNVESSLGPGDKGLIKVTYTNTGNIKVYNAEARLSAVDP
                     FSSNDDSAYLGDMEPGESVVGSYEVTVDSSATEKEYGLNTEIRYYDANDNSILSKSMK
                     AVVDIKDQSGPLAFFTSPIFIIIVVAIVLIGGYYVFMKRKGKV"
     gene            1925..2875
                     /locus_tag="O0X37_03650"
     CDS             1925..2875
                     /locus_tag="O0X37_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62052.1"
                     /translation="MGELVHANAVIEIRRVLETAGYDTEEEQGPFNLSAVRGRTCLIV
                     LCSDDLNLLQRFDMTPYTIMLEDTKVRCDKLIFTGNAYFRPKEADIWSREQLEHYIAA
                     SAMARIYSEPFDLDEPSVATYTPTAAPASTSSFTGAIGDQMLPVRITDRDAVRIAHQD
                     GPTILRMIPYWYYRYTSRGEANYKGKMVSFDDEGSGWVNAINGLETDFGDVAPVLGSI
                     PTDADVMSPVNTKAETEERVRADLVQKLTKRVRIKTTSGDAIFAEEKEFRPSPDNIEI
                     TVEKVYVPVWQVRGKKDIVEVNAYTGEELSLPSDEGCEVF"
     gene            2876..4189
                     /locus_tag="O0X37_03655"
     CDS             2876..4189
                     /locus_tag="O0X37_03655"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJK62053.1"
                     /translation="MIIVIGGGPAGRMAAVRLASAGRKVTLLERRALGGQCVHDGCML
                     ICGLNDVARSIRNTEFLRGCGVIDGSVSVRYPEVVRNLEKVQARLSQILERETLDAGV
                     KIEYGVTAEVRDGKVIVDGSVREAEAVVIACGAEINIPDVPGAGLPGTYTARTIRSMP
                     ELPKRMAIIGGGITAAEFAYIFSAFGVEVLLFARHKLLSILPDRMQKAVRRDLSSVEI
                     YEDRTLDAVLDEESVCGVVAGGETFACDAVLFATGMRPSSPHVSGVLKREDGSIVVNE
                     RMETSVPGVYACGDVTGAPYFTPVSRLQGFAAADAILGNPRTVDLSRIPFTVVLGLDY
                     TVCPCADESGATFSSPNIAGPGSVWHVSDGSVGTMELTVVPDSGKIVGFSSSGPGTGI
                     VGTYLGYLVRKGVTVHEFSPMLEVHPISDGLYSMIRFAKDHLGND"
     gene            complement(4317..4388)
                     /locus_tag="O0X37_03660"
     tRNA            complement(4317..4388)
                     /locus_tag="O0X37_03660"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4354..4356),aa:Ala,seq:cgc)
     gene            complement(4419..>4539)
                     /gene="tfb"
                     /locus_tag="O0X37_03665"
     CDS             complement(4419..>4539)
                     /gene="tfb"
                     /locus_tag="O0X37_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617032.1"
                     /note="stabilizes TBP binding to an archaeal box-A
                     promoter; responsible for recruiting RNA polymerase II to
                     the pre-initiation complex; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription initiation factor IIB"
                     /protein_id="HJK62054.1"
                     /translation="LSGERRTQREVADVAGVTEVTIRNRYKELAEQLDIEIIL"
BASE COUNT         1084 a          989 c         1199 g         1267 t
ORIGIN      
        1 gatgaagacg agctgggttt ttcctatcag gaactagatg cagcactgat gagtctggag
       61 gatcatgggt ttgtaccaca gaatgctctt gaagaacagg tccttgcgct ggtgactaaa
      121 agttcccaca aacggacttc ggcagtaagc ctgctgtaag cactgctttc gttcattttt
      181 ttgtttctgt tttgtcaagg agcattgttc cttatcccta cggatagact ctaggagata
      241 atgtggcatc gctccttagc gtaatcttat gcgaacagat ccgccagtga tctttctgaa
      301 aaacagtgcc attgcagcac ttcaaaaatt actgttttaa caaaaaagat tgggggagga
      361 ttatacctta cccttacgct tcatgaacac atagtagccg ccgattaaga caattgccac
      421 aacgataatg atgaagatcg ggctggtaaa gaatgccagc ggtcctgact gatctttgat
      481 gtcaacgaca gctttcatcg atttggagag aatgctgttg tcatttgcat catagtagcg
      541 gatttcggtg ttgagaccat actctttttc ggttgcagaa ctgtcaaccg ttacttcata
      601 gcttcccact acagactctc ccggttccat gtcgccaaga tacgctgagt catcgttgga
      661 agagaatgga tccactgcac tcagacgggc ttctgcattg tatactttga tatttcctgt
      721 attcgtgtag gtgactttga ttaagccttt gtctcccggt ccaagcgatg actctacatt
      781 ggttactttg aactcaacct ttccgtcaac cggtacccca acaattactg gggaagagac
      841 ctgatcaaca ccataattgt cctggtaaac aatctgcaaa ccaaccgggt attcattcac
      901 ctctgcattg tttgcaacag atactttgaa ggtgactgtc ttggtagatc ctgcggggaa
      961 gtcaccaata aagatactgc tctcaactgg gacgattgct gtggatgaga tacccgagct
     1021 ggaggttatc agcgatgcgt atgcgttctg tgcgtccatt cctcctgcgt ttttgatcgt
     1081 aagggtcagg aatccggtgg ttcccacgct gatatcatca caggagactt caagtacttc
     1141 tggagatacc tgggaccgaa ccgtcacatc aaggggcaca tctgctaccc gatcgttcag
     1201 ccagtagtat ccaagtgcgt cgccgtaatt ttctactgag aacagttcat tgtaaaccac
     1261 gcgaactttg agggtttgag gtcccggggt tgcatactga tcaacggaga cacggtaggt
     1321 cagagttgcg gattctcctg gtgcaatctg ggagacggtc tgcgtaccgg tctttactgt
     1381 gattccgtca gcatcctcca gcgttacgcg aagctgatat gcggttgcga catcatactg
     1441 acgttggagg ccggactctg ctgcaacaac ctcgtactgt gcattgttcg tgagtttgat
     1501 cgtaaagtct gtctcggttc cggggacaag ctcgttccct ccctcagctg ttgcgaacaa
     1561 ttcaattccc gcagttgagt acgaaacctg tgccgcagac accggtgctg caataagcac
     1621 aagcatgaga catgcggtta taatcagtgc tgatttccaa agtttcaaag aaaacaccta
     1681 atttgagtta ttctaactaa tttatgcgta tgggtgcata aatacttgct tagcgaccaa
     1741 tccagtaacg gaagaatagg tacttttctg caaaatatcc ctgaaatatg tgaagacagg
     1801 attactggta ttgaattgtt gtattagaca aagagcgaat ctaaaaatac caattaacga
     1861 attctcgggt gtcttcggtt tgcaaagtat ttttgaccac gaggtgcata gtagttacat
     1921 aagtatgggt gaacttgtac acgcaaatgc tgttattgag atacgccgcg ttcttgaaac
     1981 agctggatat gatacggagg aggagcaggg tccatttaat ctctcggccg tccggggaag
     2041 gacgtgtctc attgttctct gttcggatga tctgaatctt ctccagcggt tcgatatgac
     2101 accgtacaca ataatgctcg aggacacgaa ggtccggtgc gataaactga tcttcacggg
     2161 caatgcctat ttccgtccaa aagaggcgga catctggtcg cgtgaacagc ttgaacatta
     2221 tattgctgcc tctgcgatgg cacggattta ctcagagccg tttgatctgg atgaaccatc
     2281 tgttgctact tatactccca cagccgctcc tgcgtccacc tcatctttta caggggcaat
     2341 tggtgatcag atgttgcctg tcagaatcac agatcgtgat gcagtgcgga ttgcccatca
     2401 ggatggacct acgatacttc gcatgattcc ctactggtat taccggtaca caagccgtgg
     2461 tgaggcaaac tacaagggaa agatggtgag ctttgatgat gaggggtctg gatgggtgaa
     2521 tgcaatcaat ggtcttgaga ctgattttgg tgatgtagca ccggttttgg gttcgatacc
     2581 tacagatgca gacgtaatga gcccggtaaa tactaaggcg gaaaccgagg agcgtgtccg
     2641 cgctgatctt gtccagaaac tgacaaaacg tgtgcggatc aagacaacct ccggcgatgc
     2701 aatttttgcg gaagaaaagg agttccgtcc gtcaccggat aatattgaga ttactgttga
     2761 gaaagtgtat gttcctgtgt ggcaggtccg cggcaagaaa gatatcgttg aggtgaatgc
     2821 gtacaccggc gaggagttat cactgccgtc ggatgaagga tgtgaagtgt tttaaatgat
     2881 tatcgtcatc ggtggaggtc cggcgggcag gatggcagct gtgcgtcttg cctctgccgg
     2941 tcgaaaggtt actcttttgg aacggcgggc actaggaggg cagtgtgtgc acgacgggtg
     3001 tatgctgatc tgtgggttga atgatgtcgc cagatcgatc cgaaatactg aattcttacg
     3061 cggctgtgga gtgattgacg gttcggtctc tgtcaggtat cccgaagtgg tgcgcaatct
     3121 tgagaaagtt caggcacggt tatcccagat tcttgaacgt gagacgcttg atgccggagt
     3181 gaagatcgag tacggtgtaa cggccgaggt tcgcgacggt aaagtgattg tggatggatc
     3241 tgtacgtgag gctgaggcgg tggttattgc atgcggtgct gagataaata ttccggatgt
     3301 tccaggtgca gggttgccgg ggacttatac cgcacggaca attcgatcaa tgcctgaact
     3361 cccgaaacgt atggcaatca ttggtggagg tattacggct gcagagtttg cctatatctt
     3421 ttcagcattt ggtgttgagg ttctgctttt cgcccggcac aaactgttgt caatccttcc
     3481 cgatcggatg cagaaagctg tacggcgtga cctgtcatcg gttgaaattt acgaggatcg
     3541 tacacttgat gcagtattgg atgaggaatc ggtttgcgga gttgttgcgg ggggtgagac
     3601 gtttgcgtgt gatgccgtgt tgtttgcaac gggaatgcgt ccgtccagtc cccatgtatc
     3661 tggtgttctg aaacgggaag atggatctat tgtggtgaat gagcgtatgg aaacgtcggt
     3721 tcctggtgtg tatgcctgcg gtgatgtaac cggtgctccg tactttacac cagtgtcacg
     3781 gttgcaaggg tttgctgcag ctgatgcaat tcttggcaat ccgcggacgg tggatctgtc
     3841 ccgtattccg tttaccgttg tgcttgggct ggattacacg gtttgcccgt gtgctgatga
     3901 atccggtgca acattttcct ctccgaatat tgcaggtccc gggtctgtgt ggcatgtttc
     3961 agacgggtct gtagggacaa tggagctcac tgtagtaccg gatagcggaa agattgtggg
     4021 tttttccagt tctgggccgg gtacaggtat tgtgggaacg tatcttggat acctggtgcg
     4081 aaaaggagta acggtgcatg agttttcccc gatgcttgag gttcacccaa tttcagatgg
     4141 cttgtattct atgatacggt ttgcaaagga tcatctcggg aatgactgag tttcaattat
     4201 ttttgtgatt cctgcatatc ttccctttag gagtacgctc taactatacc cgttttttgc
     4261 cacaatgctt ttctggcgat aacgttactt ccaaattttt ttgaaaaacg aaatgatgga
     4321 cccggcggga tttgaacccg cggcatctac gttgcgaacg tagctatcta cccctgatat
     4381 acgagcccga taaaatgtat cggggaaaag aaatcccctt agagaataat ctcaatatct
     4441 aattgttctg ccaattcctt atatctgttc cgaatcgtga cctctgtcac acctgcaaca
     4501 tccgcgacct cacgctgggt tctccgttct cctgagaga
//
LOCUS       DALUAJ010000173         7548 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_32104,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000173 DALUAJ010000000
VERSION     DALUAJ010000173.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 7548)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7548)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7548
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32104"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            228..1451
                     /locus_tag="O0X37_03670"
     CDS             228..1451
                     /locus_tag="O0X37_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HJK62055.1"
                     /translation="MVGRHYDCIIIGAGPAGLFCASLLAPARVLVLEKMVLPGRKLLI
                     AGSGQCNITHTGDVKNFVSHYGEHGAFLRPALMAFPNTAVREFFERLGVSFVEKESGK
                     VFPESHSADDILDALLDACDAAGVEILSYTPAESVACENGMYAVKTLHTTYYAKILVI
                     ATGGMSYPQTGSTGDGFLFAKALGHSVTPPCPALTPVYAVRHQLADLSGISVYGATIS
                     IWRGGKKLLVRSGDLLITRFGYSGPVILDSARWMRTGDILRIGFCKKKPEELDTLIRA
                     ACSSAGTKQIQNLLDDAECPDRLVKHMVQESGIPEGTTGAQLTASQRSRLVENLTSYP
                     VEIDHLGDFRVAMATAGGVVLDEINKKTCESKISPGLFFIGEVLDIDGDTGGYNLQAC
                     FSTAFLSAQRIRQIL"
     gene            1569..1641
                     /locus_tag="O0X37_03675"
     tRNA            1569..1641
                     /locus_tag="O0X37_03675"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1602..1604,aa:Thr,seq:ggt)
     gene            1785..2693
                     /locus_tag="O0X37_03680"
     CDS             1785..2693
                     /locus_tag="O0X37_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HJK62056.1"
                     /translation="MKKTMMIIAAVLLAAVLLVGSGSAVDKVVTGIDDLSGAVIGVQL
                     GTTGDELVTAFEGDANGTKIERYNKGADAVQALKTGKIDCVVIDLLPAQSFVEKNSDL
                     MILSEGFDQEEYALVVKKGNTELVDAINRALAELKADGTIDQINANFIPTSELKGTCP
                     YVSPEGIERPNGKLVVATNAEFPPYEYWESGKITGIDMQIMQAICDKLGYEMKIEDMA
                     FDSIIAAVSSGKADVGAAGMSVTEDRMKNVDFSDPYTTAQQVIIVKKPVTSTTEPGEA
                     TPTKAPAPVLGILAGLGAAVVLLRKF"
     gene            2808..3713
                     /locus_tag="O0X37_03685"
     CDS             2808..3713
                     /locus_tag="O0X37_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HJK62057.1"
                     /translation="MCNKTMKNVLERVLFLALTVLLVSSMFMGAVSAATDNNTGNENS
                     GTTVSEEPMTIFGDISSAVIQYFEGLKLFFEELPDKLYQNFIEDDRYKYLIEGFCNTI
                     VITIFALILGLVLGTCIAIVRTIHDLHGKLKVANAICKVYLAVIRGTPVMLQLLIIYF
                     VIFAAVDVDKVLVAVVAFGINSAAYVAEIIRAGINAVPKGQFEAGASLGLPFKLTMMT
                     IILPQAVKNILPALCNEGITLMKETAISGYIGLVDLTKAGDIIRSQTWEAFIPLIAVA
                     LVYLVMVLILTHLVQKLERRLNNAN"
     gene            3703..4446
                     /locus_tag="O0X37_03690"
     CDS             3703..4446
                     /locus_tag="O0X37_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HJK62058.1"
                     /translation="MLTEGEVLIEVNDLRKSFGDHEVLKGISTTIHAGEVVAIIGPSG
                     CGKSTFLRSLNLLEIPTGGTILFEGTNITDKSVDVNKMREKIGMVFQQFNLFPNMTVK
                     ENIMLAPTRLGKMTKEEAEKKAENLLQRIGLLDKADVHPQKLSGGQQQRVAIIRALAM
                     NPDVMLFDEPTSALDPEMVGEVLTLMKQLADEGMTMVIVTHEMGFAREVADRILFIND
                     GVIQEENTPEEFFTHPKNQRLQEFLSKVL"
     gene            4559..4906
                     /locus_tag="O0X37_03695"
     CDS             4559..4906
                     /locus_tag="O0X37_03695"
                     /inference="COORDINATES: protein motif:HMM:NF018637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HJK62059.1"
                     /translation="MEIFETKTPSASDPENMRITLLISRLKEHGFSIERYNQRTSPKK
                     FQENHTVEKLITTHGKQICPITLVDGFAMITGRYPSNEEIRQILTVPASLIAEKNEGC
                     CCIQGCNREEKIV"
     gene            4903..5250
                     /locus_tag="O0X37_03700"
     CDS             4903..5250
                     /locus_tag="O0X37_03700"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HJK62060.1"
                     /translation="MTEQIFKALSDRTRLRMVSLVMKRELCVCEIMEVLNLSQSNASR
                     HLTILKNAGVLSGRKQAQWAYYKISEMLPPELYQYLSRTFSEDPLYAEDRKRLETCTV
                     ADGCKHTTTGDSP"
     gene            5369..6253
                     /locus_tag="O0X37_03705"
     CDS             5369..6253
                     /locus_tag="O0X37_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014865920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HJK62061.1"
                     /translation="MSLADTLVSAGEYFLIITAELVLLFVVVSLFVGVLQVYVSPRTM
                     QRVLGNTGSVAGSMFGAAFGALTPFCSCSTIPITLGFLKSGVAFSSSMSFLFASPLLN
                     PIILAMMLVIFGPIITVVYAVIMFVFAVGIGLILERAGYRKYLKPVAVEGGIDQAGTK
                     FQQIVNFALTIFRQMLPYLLLGAGIGAFIYGFLPSDWVLAAAGPENIFAIPIAALIGI
                     PLYIRAETILPIAAVFLEKGMGTGTVAALLIGGAGMSIPEITMLSAIFEKKLVGVFVI
                     MIFASAVLTGCILQIITW"
     gene            6261..6968
                     /locus_tag="O0X37_03710"
     CDS             6261..6968
                     /locus_tag="O0X37_03710"
                     /inference="COORDINATES: protein motif:HMM:NF040157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HJK62062.1"
                     /translation="MQNDSTEPACLICGAPLRYLECAREMTCAICGKICLSNAECIHG
                     HFVCDDCHSTPAAVVIRSIALAASEKDPFVIATAMMKSPAVHMHGPEHHILAGASLLA
                     AYRNAGGELDLPTAVEEMLRRGTMIPGGFCGLAGTCGAAISAGMFYSIVTKTNPLSKE
                     TWRDAQLLTAACLTAIGSHGGPRCCKRDTYLALETTVRFMREHTGIAMEWQRKPQCIF
                     SQRNRECLKETCPYFPE"
     gene            complement(7029..7262)
                     /locus_tag="O0X37_03715"
     CDS             complement(7029..7262)
                     /locus_tag="O0X37_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62063.1"
                     /translation="MAETDDVDLIVPPGTPRTIIRDIALTFDVEMVTVTRPLNYANMV
                     NDERELIAFRGKPDEVKKVEAFLVEKLKEFIES"
     gene            complement(7269..>7548)
                     /locus_tag="O0X37_03720"
     CDS             complement(7269..>7548)
                     /locus_tag="O0X37_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004038393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2114 family protein"
                     /protein_id="HJK62064.1"
                     /translation="CSERGLLPPNSSIGFTGRAIISGNKPQYILDGVAERGLYDDPVT
                     HLVFVDDGLARGAALMGRCMNSLGHPKCPIGGVRSGKCIMAKRQKIGR"
BASE COUNT         2153 a         1653 c         1823 g         1919 t
ORIGIN      
        1 aaacagccgt aagtcacgaa aagagcagct gcaacaagta catctgttgc aaacaagcat
       61 tcccgcagac attctgcgga gccgaactga ctcatcgtaa tcccaaagcc aagaatggca
      121 gtgtcgtaac agacagacac gtcggactta cttcagccag cactgtaaag tgcttcttct
      181 atataggaga gaagatgaga taagggttgc gggaaaatat actgggtatg gtaggccggc
      241 actatgactg tatcattatc ggggcaggac cagcaggtct tttctgtgca tcacttcttg
      301 ctcctgcgag ggtattggtc ttggaaaaga tggtacttcc gggacgaaaa ctgctgattg
      361 cagggtcagg ccagtgcaat attacgcata ccggggacgt gaaaaatttt gtttcccatt
      421 atggagaaca cggtgcattt ttgcggcctg cattaatggc attccccaac acggcagtgc
      481 gagaattctt tgagagatta ggagtttcat tcgtggaaaa ggaatcggga aaggtgtttc
      541 cggaatcaca ctccgcagac gatattttag acgcattgct ggatgcctgc gatgcagcag
      601 gcgttgagat tctgagttac acgcctgcag aaagcgttgc ctgtgaaaac ggaatgtatg
      661 cagtaaaaac gctgcatacg acatattatg caaaaatact tgtcattgca acaggaggta
      721 tgtcatatcc acagacagga tcaacaggag acggattttt gtttgcgaaa gcacttggcc
      781 actccgttac accaccctgc ccggcactaa caccggtcta tgcggtacgg caccagttag
      841 ctgatttgtc cgggatttct gtatacggag caacgatatc aatctggcgc gggggaaaga
      901 aactgcttgt gcgttccgga gatctgctga tcaccagatt tggttattcc ggaccggtga
      961 ttctggattc tgcccgatgg atgcgaacag gagacatact aaggattgga ttttgtaaaa
     1021 agaaaccgga agagctggat acactgatcc gtgccgcgtg ttcttctgcg ggaacgaaac
     1081 aaatacagaa tttgctggat gatgctgaat gcccggaccg gttggtgaaa cacatggtac
     1141 aggaatcagg aattcctgag ggaacaacag gagcccagct cacggcatca caacggagca
     1201 gactggtaga aaatctcacg tcttatcctg tagagattga tcacctggga gatttccggg
     1261 tggcaatggc cactgcggga ggggtcgtac tggatgagat caacaaaaag acgtgtgagt
     1321 caaaaatatc tccaggtcta tttttcattg gcgaggtttt ggatatcgat ggcgatacag
     1381 ggggttacaa tctacaggca tgtttttcga cggcattcct ttcagcgcag cgtattcgac
     1441 agattctctg atttttcgaa ttgacgcacc tgttttttca gttagaagaa aatgttacat
     1501 gtacagccca gagactttca atccataatg tttaaatctg caaacttcct actttgttat
     1561 cacgcaatgc ctcagtggct tagccggcat agcgcgtcct tggtaaggac gaggtcgcga
     1621 gttcaaatct cgcctgaggc tttttctttt cttacgattt gtttcatcag gtacgtctgc
     1681 tgagatagct actaaatctc ctaaatcctg agtaatcaat aactcgaaag actcatataa
     1741 aacgcgaaat acatgttcat gtgtattaga ggtgtgttta gactatgaaa aaaaccatga
     1801 tgatcattgc ggcagttctt cttgccgcag tccttctggt aggatccgga tctgccgtag
     1861 acaaagtagt tacggggata gatgacctct cgggagcagt aattggtgtt cagctgggaa
     1921 caaccgggga cgaactggta accgcttttg aaggtgacgc caatggcacg aaaatcgagc
     1981 gctacaacaa aggtgctgac gcagtgcagg cattaaaaac cgggaaaatc gactgtgtcg
     2041 taattgactt gttaccagcc caatccttcg tggaaaaaaa cagcgatctg atgatccttt
     2101 cggaaggctt tgatcaggaa gagtatgcgc tggttgttaa aaaaggaaac acagaactcg
     2161 ttgatgctat caacagagcg cttgctgaac tgaaagcaga cggaacaatt gaccagatca
     2221 atgcgaactt tatcccaacc tcagagctga aaggtacctg cccgtatgtg tcgcctgagg
     2281 gtattgaacg accaaacgga aagctggttg ttgcaaccaa tgcggagttc ccgccatacg
     2341 agtactggga gtctggaaag atcactggaa ttgacatgca gatcatgcag gcgatctgtg
     2401 ataaactcgg ttatgagatg aagattgagg acatggcatt tgactccatc attgcagcag
     2461 tttcctcagg aaaagcagat gttggtgcag caggaatgtc agttacggaa gacagaatga
     2521 agaatgtaga cttctctgat ccgtacacaa ctgcacagca ggttatcatc gtgaaaaaac
     2581 cggtaaccag tacgactgaa cccggagaag ctaccccgac caaagcaccc gctccggttt
     2641 tgggaattct tgcaggtctt ggtgctgcag ttgtacttct gagaaaattc taaccttttt
     2701 tttctaaaac acagaagcac agactcagga ctgcggcaat agataagtaa taattcttat
     2761 gtctttagag aacgcacgtt accgatacgt ttaccggggg tctctgaatg tgtaacaaaa
     2821 caatgaaaaa cgtccttgaa cgggttctct tccttgcatt gacggtgctt ttggtatcat
     2881 cgatgtttat gggggctgta tccgccgcaa cagacaataa tacaggaaat gaaaacagtg
     2941 gaactaccgt ctccgaagag ccgatgacaa tctttggaga catctccagt gcagtaatcc
     3001 aatactttga aggactaaag ttgttttttg aagagctccc cgacaaactt tatcagaact
     3061 tcattgaaga tgaccggtac aaatatctca tcgaaggttt ttgcaacacc atagtcatta
     3121 cgatatttgc cctgatcctt ggactggtgc tcggtacctg catagccatt gtcagaacga
     3181 tccacgatct ccatggaaaa ctaaaggtag caaacgctat ctgcaaagta tatcttgctg
     3241 ttatccgggg gacaccggta atgctacaac tgcttatcat ctactttgtg atatttgccg
     3301 cggtggatgt ggataaagta ctggtggcag tggtggcgtt tggaattaat tctgcagctt
     3361 acgtggcaga gattattcgt gctggtatca acgcagttcc caaaggacag tttgaagcag
     3421 gagcaagtct tggactgccg ttcaaactta cgatgatgac cattatcctg ccgcaggcag
     3481 ttaaaaacat tctaccggca ctgtgcaatg aaggtatcac actgatgaag gaaactgcaa
     3541 tcagcggata tattggtctc gttgatctga ccaaagccgg agatattatc agaagccaga
     3601 cctgggaagc cttcattcct ctgatcgcgg ttgctctggt gtatctggtg atggttttga
     3661 ttctgacaca tctggtacag aagctggagc ggaggttaaa caatgctaac tgaaggtgag
     3721 gtattaatcg aggtcaatga cctcagaaaa tcattcggcg atcacgaggt cctgaaagga
     3781 atctcaacca ccattcatgc aggagaagta gtcgccatta ttggaccgtc ggggtgtgga
     3841 aaatccacgt ttcttcgttc actaaatctt ctggagattc caaccggagg aacaattctc
     3901 tttgaaggaa caaatattac ggataaatcc gttgacgtca ataagatgcg cgaaaagatc
     3961 ggaatggtgt tccagcagtt caacctcttc ccaaatatga cggtcaaaga aaatatcatg
     4021 cttgctccaa ccagactggg caagatgaca aaagaggagg cggaaaagaa agctgaaaac
     4081 ctcttgcaga ggatcggcct tttggacaaa gcagatgtac atccgcaaaa gctgtcaggt
     4141 gggcagcagc agcgcgttgc tatcatccgg gcactggcca tgaacccaga tgtgatgttg
     4201 tttgatgaac caacctcagc acttgatcca gagatggttg gtgaggtctt aactctcatg
     4261 aaacagcttg cagatgaagg gatgacaatg gtgatcgtca cgcatgagat gggatttgca
     4321 cgggaggtgg cagatcggat tctgttcatc aacgatggtg taatccagga agaaaataca
     4381 cccgaagagt tctttacaca cccgaaaaat cagagactgc aggagttcct ttccaaagtc
     4441 ctttgagagc actgaattaa aaccccatct cattttttcc actaacattc ggttcaatag
     4501 atctctcttt cgaaaaccat catatgcaca tatgttcata taatgattta tggtgaagat
     4561 ggaaatcttt gaaacaaaaa caccatcagc atcagaccct gaaaatatgc ggataacatt
     4621 gttgatttcc cgcctcaaag aacatggatt cagtattgag cggtataatc agagaacctc
     4681 tccaaaaaaa tttcaggaga atcacacagt agagaagctg atcacgacac atggcaaaca
     4741 gatttgccca ataacactag tagacggttt tgcaatgatt acaggacgtt acccgtccaa
     4801 cgaggaaata cgccagatcc ttacagttcc tgccagcctc attgcagaaa aaaatgaagg
     4861 atgctgttgt attcaagggt gtaaccgaga ggagaaaata gtatgactga acagatattc
     4921 aaagctctga gtgacagaac aagacttcgt atggttagtc tggtaatgaa acgtgaactg
     4981 tgtgtctgtg agattatgga agtcctgaat ctctctcaat caaatgcatc acgacatttg
     5041 accattctca aaaatgcagg agtactctcc gggagaaagc aggcacagtg ggcatactac
     5101 aaaatcagcg aaatgctgcc tccggaactg taccagtatc tttcacgaac attttccgaa
     5161 gatcccctct atgcagaaga tagaaaacgg ctggaaacct gcactgtagc agacggatgt
     5221 aaacatacaa ccacgggtga ttcgccatga aagaagaaaa agggattttt aaaaagttga
     5281 aaaaagctgt taccggagaa caatgctgtt gctgcaatat taaaattgta cctgagacgt
     5341 ctgaagataa caaagaggag aacactaaat gagtcttgcc gacacattag tttctgctgg
     5401 agagtatttt ctcatcatta cagcagaact ggtacttctt tttgttgtag tcagtttgtt
     5461 cgtgggagta ttacaggtgt acgtttcacc gcgtacaatg cagcgagtgc ttggaaatac
     5521 agggagcgta gcaggaagca tgtttggggc agcatttggt gcactcacac cattctgttc
     5581 ttgttccacc atcccaatca cgcttggctt tttgaaatcc ggggtcgcat tttcaagttc
     5641 gatgtcattt ctatttgcct ccccattact aaatcctata attttagcaa tgatgttggt
     5701 cattttcgga ccaataatta ctgttgtcta tgcagtgatt atgtttgtct ttgctgtggg
     5761 aatcggtctt attttggaac gcgcaggata ccggaaatat ctcaaaccag ttgctgttga
     5821 gggtgggata gatcaagctg gtacaaagtt tcagcagatt gtcaacttcg cactcaccat
     5881 tttccggcaa atgttgccgt atcttctgct gggagcaggt atcggggcat tcatctatgg
     5941 atttcttccc tcagactggg tattggcagc agcaggcccg gagaatatct ttgcaattcc
     6001 aatagctgca ctcattggga tccctttata catccgggca gaaacaatac tacctatcgc
     6061 agcagtcttt cttgagaaag ggatgggtac cggaacggtt gctgccctcc ttattggagg
     6121 tgccgggatg agtattccag aaatcactat gctatctgca atatttgaaa agaaacttgt
     6181 gggtgtattt gtgataatga tatttgcttc agccgtactc actggctgta tccttcagat
     6241 aataacttgg tgatgcaaaa atgcaaaatg attcaacaga acctgcctgc ctcatctgcg
     6301 gggcccctct ccggtacctt gaatgtgcgc gcgagatgac ctgtgctatt tgtggaaaaa
     6361 tctgcctcag caatgcggag tgtattcacg gtcactttgt ctgtgatgac tgccactcaa
     6421 cccctgcagc agtggtaatc cgtagtattg cattggcagc atcagaaaaa gatccgtttg
     6481 taattgcaac ggcaatgatg aagagtcccg ctgtacatat gcacggaccc gaacaccaca
     6541 tcttggcagg agcctcactt cttgcagcgt atcggaatgc aggaggagaa ctggatcttc
     6601 ccacagcagt agaggagatg cttcgccgcg gtacaatgat cccgggtgga ttctgcggac
     6661 ttgcaggaac ctgtggggct gcgatcagcg cagggatgtt ttactccatt gtaacgaaaa
     6721 caaatcccct gagcaaagaa acttggagag atgcccagct actaactgca gcatgtctta
     6781 ctgcaattgg cagtcacgga gggccgcgct gctgtaaacg cgacacatat cttgctcttg
     6841 agacaacagt tcggtttatg cgggaacaca ccggtattgc aatggaatgg caaagaaaac
     6901 ctcagtgtat cttttcccaa agaaatcgtg aatgtctgaa ggaaacgtgc ccatatttcc
     6961 cggaataaac agcatatcac acacaggaga gtactggagc aaaaaaagaa aatgaaagag
     7021 aagtaaattc agctctcaat aaattccttg agcttttcca caaggaatgc ttccactttt
     7081 ttgacctcat caggttttcc gcggaatgca attagctcac gttcatcatt gaccatgttg
     7141 gcatagttca gcggtcgcgt aactgtaacc atctccacgt caaacgtgag ggcaatgtcc
     7201 cgaataattg tacggggagt tcccggagga acgattaaat ccacatcgtc tgtttccgcc
     7261 atatcagctt acctcccaat cttctgacgc ttagccataa tacactttcc actgcgaaca
     7321 ccgccaatcg gacattttgg atgaccaagg ctgttcatac atctgcccat cagggcggca
     7381 ccgcgtgcca gaccatcatc cacaaagaca aggtgcgtta ctggatcgtc gtaaagccca
     7441 cgctctgcaa caccgtccag aatgtactgc ggtttgttcc ccgagatgat agcacgaccg
     7501 gtaaacccga tggaactgtt tggcggaagc agtccgcgtt cgctgcac
//
LOCUS       DALUAJ010000174         2883 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_32239,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000174 DALUAJ010000000
VERSION     DALUAJ010000174.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32239"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            93..836
                     /locus_tag="O0X37_03725"
     CDS             93..836
                     /locus_tag="O0X37_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HJK62065.1"
                     /translation="MPQIDIVLVEPLYEGNIGFAARVMKNFGFHHMVLVNPPKLTVEA
                     TARASHAKDVLESAEILTLDEVFARSNLAVATTGGLSKSVSNPMRMPYYSPAELREMI
                     KDVDGRISILFGRENWGLNNDEIRRCDIVVTIPTSPEYPIMNISHAIGVVCYELAHLP
                     RGEYMLASRVEMDALYAHFERFLEGIGHPIEKRETTLLLAKRVLGRTNLTVREASTIH
                     GILRRTEWHLKHPGVAYDDKGREYWDGEE"
     gene            complement(868..2397)
                     /locus_tag="O0X37_03730"
     CDS             complement(868..2397)
                     /locus_tag="O0X37_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HJK62066.1"
                     /translation="MTWTADTWIALFLILMYFALLLGIGVWAAKRIKNSEDYILAGRS
                     LGFWIFVLLLITSICSGVTLIGGSGMGFTYGWPSIWDQIFVPLSAVFCIVFFGSKLNL
                     IGKKQGIVTLEDYFSLRYENNRELRTISAVIGILVSLVYLVGQYTAISIVLVWLFGME
                     HWQALLIAAAIITTYTILGGFYAVSWTGMVQGLILIFGMLISAPMIVMYAGGLEHINT
                     VLAAIDPNFVMPAFPERYAAYAYITPEIIFSFGVLLVVGLACAPHVVSNVLAVKDVRF
                     FRWAPLIAFAVYLTISMLVRFSGMGVRTMVEEGTVVLPDVTNAADYSFVYGISAAAGS
                     VFAMGLFAVMILAAVMSTTDRLMLTVGTQFSWNIFKVILHPKATEKQVIRVSQITMLA
                     AVIISLLLAVNPPDVLVFLIFLAIGLILASFAVPLIAGMYWRRATTAGAVASMVFGLA
                     AALCFGYYDQFVEKLPMHFSIYALLLSIAAMVIASLLTKKNSDTALDKTYTGWYLHPK
                     K"
     gene            2521..>2883
                     /locus_tag="O0X37_03735"
     CDS             2521..>2883
                     /locus_tag="O0X37_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF126 domain-containing protein"
                     /protein_id="HJK62067.1"
                     /translation="MILKGRSIAKGTGSGELLYTDTPISFLGGVDAATGIIIDEKHPL
                     HGQSVAGKVLAFPYGKGSTVGSYVMYGLARNNVAPAAIINTECETIIAIGAIISGIPM
                     VDRLDGDVSALSGTITVNG"
BASE COUNT          771 a          718 c          775 g          619 t
ORIGIN      
        1 gccagtatgc ggatgcaatt gttgtgggca acgcagtata cgatgcagga actgcggttc
       61 tcaaagaaac ggtgaaggcg gtcagacgct aaatgccaca gatcgatatc gtacttgttg
      121 agccgctgta cgagggaaac atcgggtttg ccgcacgggt catgaagaac ttcgggtttc
      181 accatatggt gctggtaaat ccgccgaagc tgacggtgga agcaaccgcc cgggcatcgc
      241 atgcaaagga tgttcttgaa tcagctgaga ttctgacact ggacgaggtg tttgcccgca
      301 gtaatcttgc agttgcaaca accggcggcc tgtcaaagtc tgtgtcaaat ccgatgcgga
      361 tgccctacta ctctccggca gagctgcggg agatgatcaa ggatgtggac ggacggatct
      421 cgattctgtt cgggcgggag aactgggggt taaacaatga tgagattcgc cggtgtgaca
      481 ttgttgtaac gattcctacc tctcccgagt atccgattat gaacatctcg catgcgatcg
      541 gcgttgtctg ttatgaatta gcacatcttc cccgcggcga gtacatgctt gccagccggg
      601 ttgagatgga tgccctctac gcacacttcg aacggttcct ggaaggtatc ggacatccta
      661 ttgaaaaacg ggagacgacc cttcttcttg caaagcgggt tttgggcaga acgaatctga
      721 cggtccgtga ggcaagcaca attcacggaa ttctccgccg tacggagtgg cacctgaaac
      781 atccaggcgt agcctacgat gataagggcc gtgaatactg ggacggagaa gaataatttt
      841 tcagaagaat acaatttttt cttttgatca cttcttggga tgcagatacc agccggtata
      901 ggttttgtcc aatgcggtat ccgagttctt ttttgtcaga aggcttgcga tcaccattgc
      961 cgcaatggag agtaataatg catagatgga gaagtgcatc ggcagctttt ccacaaactg
     1021 atcatagtag ccgaagcaca gtgctgcggc aagtccaaaa accatactgg ccacggcacc
     1081 tgcggtagtg gcccggcgcc agtacatccc tgcaatcagc gggaccgcaa aggatgcaag
     1141 gatcaatcca atggcaagaa agatcagaaa taccagaacg tccggcggat ttactgcaag
     1201 cagaagggag atgatcaccg cggcgagcat ggttatctga ctgacgcgga tgacctgttt
     1261 ctccgttgct ttgggatgca aaatcacctt gaaaatgttc caggagaact gtgtgccaac
     1321 cgtgagcatc agccgatcgg tggtggacat gaccgcggca agaatcatca ccgcaaacag
     1381 tcccatggca aatacactcc cagccgcagc actgatgccg tacacaaacg agtagtcggc
     1441 ggcattggtt acgtcaggca gaacaaccgt tccctcctca accatggtcc tgacacccat
     1501 tcccgagaac cggaccagca tggagatggt gaggtacacg gcaaatgcaa tgagcggtgc
     1561 ccagcggaaa aaacgaacat ctttgaccgc aaggacattg gataccacgt ggggagcaca
     1621 ggcaaggccg accaccagca aaacgccgaa ggaaaagatg atctccggag tgatgtaggc
     1681 atatgccgca taccgctccg ggaatgcagg catcacaaaa ttgggatcaa ttgcagctag
     1741 gacggtgttg atgtgttcaa gtcctccggc atacatcacg atcatcggtg cggagatgag
     1801 catcccgaag atgagaatca gcccctgaac catgcctgtc caggacacgg catagaaacc
     1861 gccaagaatg gtatacgtgg tgataatggc agcggcaatc aggagtgcct gccagtgttc
     1921 catcccaaac agccagacaa ggacgatgct gatggcggtg tactgaccta ccagatacac
     1981 cagagaaaca agaatgccaa tgaccgcaga gatcgtccgg agctcacgat tattttcata
     2041 tctgagggag aagtaatctt ccagcgtaac aattccctgt ttttttccga tcagattcag
     2101 tttcgatcca aagaatacaa tacagaacac cgcagacagc ggcacaaaga tctgatccca
     2161 gatgcttggc cagccgtagg tgaaccccat cccgcttccg ccgataaggg tgactccgct
     2221 gcagatggat gttatcagca gcaggacaaa tatccagaac ccaagacttc gtccggcaag
     2281 gatgtagtcc tcggaatttt tgattcgctt tgcggcccag acaccgatgc caagcaggag
     2341 ggcaaagtac atcagaatca aaaaaagggc aatccaggtg tcagctgtcc aggtcatagc
     2401 agtccgtttg ttgcagagtt tgtgaacgaa aatttcgtta ccgaatttgt cggttgtata
     2461 gatataagag tgtaatggtt ttgtgaggct ttaatgccgg agaggcacat atatagaggt
     2521 atgattctga aaggccgcag cattgccaaa ggtacgggat ccggcgagct tctctatacc
     2581 gatacaccca tctccttcct tggcggcgtt gatgccgcaa caggcattat catcgatgaa
     2641 aaacatccgc tgcacggaca atccgttgca ggaaaggttc tcgcattccc ctacggaaag
     2701 gggtccaccg ttggctcata cgttatgtac ggccttgcaa gaaacaacgt ggcaccggct
     2761 gcaatcatca acaccgagtg cgagaccatc atcgcaattg gtgcgatcat ctccggcatt
     2821 ccgatggttg accggctgga tggtgatgta tcggccctct ccggaacgat caccgtcaac
     2881 ggc
//
LOCUS       DALUAJ010000175         2374 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_33080,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000175 DALUAJ010000000
VERSION     DALUAJ010000175.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33080"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="O0X37_03740"
     CDS             <1..444
                     /locus_tag="O0X37_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HJK62068.1"
                     /translation="TLRVTQYLLRDIGEEIAALELVADMIRTHPALVTYNGRSFDLPF
                     TNSRLSYYGERECRPALHFDLLHTSRRLFRSDLPDCCLGTVERQILGFGRTEDLPGYL
                     VPVYYQRYLRTGDTSPLKQIVDHNRSDVTTLALLLAKQTEMMYGS"
     gene            434..>2374
                     /locus_tag="O0X37_03745"
     CDS             434..>2374
                     /locus_tag="O0X37_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJK62069.1"
                     /translation="MDPEMYTRVFLARDAVYKEPETLPSPVICTYLKSHNIRLYTHQA
                     DSYDAVMRGANIILTTPTASGKTLAYTLPVLEKLMQNRDATALFIYPTKALARDQLGV
                     LQELDMDLVAKTRPAIYDGDTPRDARAKIRSSSRIILTNMYELHQILAWRMKWGDFWA
                     NLSFVIIDEAHRYRGIFGSHIALVLRRMRRICNYYDAHPQFILSSATIGDAPSFAETL
                     TGVTAVEIANDGSPRAQQTFRLYNPWCSGKSSLSATADLIQDQIRSGMQTLCFTKSRN
                     MAEITALRCRESLPSSSISSYRGGYRPNERRVIENDLKKGDIAGVISTNALELGIDVG
                     GLDSVIICGFPGTMMSVRQQAGRAGRGGKDALITFIANLNPLDQYFMRCPDAFFDAPH
                     ERPILDLENPYLLREHILCAAAELPYRTERDGEYFGPLAAEIVRSLKEEHLLASTPKG
                     YVYCGTEPPSQQVSLSGRTSGTCTIMHGSRVLETMDTSQMFREAYPGAVLYHQSERYR
                     VEEVDRKNLIVHVKNITENYHTRPFYTTDVRVLSCEKTYRHGSLLVYYGSVSVCSQMI
                     GYSVLEYDQIVSTHSLDVPPLSFTTKACWFVPDCGNEIHPSEIAGSLHGTEHALIAAM
                     PVHVLCDRSDIGGVSNPFHSDVG"
BASE COUNT          539 a          635 c          578 g          622 t
ORIGIN      
        1 acccttcggg ttactcagta tctgcttcgc gacattggcg aggagatcgc agcactcgaa
       61 ctcgtcgcgg atatgatacg tacccatcca gctcttgtca cctacaacgg gagatcgttt
      121 gatctgccgt ttaccaacag tcgcctctcg tattatggag agcgggagtg ccggcctgca
      181 ttgcactttg atctgttaca cacgtcaaga cgcctgtttc gttcggatct gccagactgc
      241 tgtcttggaa cggtggaaag acagatcctc gggtttggcc gtactgagga ccttccaggt
      301 tatcttgttc ctgtctatta tcagaggtat ctgcgtactg gtgatacctc tcctctcaag
      361 cagatcgttg atcacaaccg gagcgatgtt accacccttg ccctgttact tgcaaaacaa
      421 acggagatga tgtatggatc ctgagatgta cacacgtgtg ttcctggcca gggatgcagt
      481 atacaaagag ccggaaacgt taccgtctcc ggtgatttgc acatatttga aaagccacaa
      541 tattcgtctc tacactcatc aggcagactc ctatgatgcg gtcatgcggg gtgcaaatat
      601 aatcctcacc actccgaccg cctcaggaaa aactcttgca tacactctgc cggttctgga
      661 aaaactgatg cagaaccgtg atgctactgc gttgttcatc tatcctacca aggctctggc
      721 ccgtgatcag cttggtgttc tgcaagaact cgacatggat cttgttgcca aaacccgccc
      781 tgcaatctac gacggtgaca ctccccgcga tgccagggca aaaatccgca gtagttcccg
      841 gattattctc accaacatgt atgaactgca tcagattctt gcatggagaa tgaagtgggg
      901 tgacttctgg gcgaatctct cctttgtcat tatcgacgag gcacatcggt accgggggat
      961 atttggttcg catattgctc ttgttttacg gaggatgcgg cgtatctgta attattatga
     1021 cgcccacccg cagtttattc tctcctccgc aaccatcggt gatgccccgt cgtttgccga
     1081 aactctcact ggtgttacag ctgttgagat tgcaaacgat ggctctcccc gggcgcaaca
     1141 aacctttcgg ttgtacaatc cgtggtgttc gggaaaaagc agcctttctg caactgctga
     1201 tctcatccag gatcagatcc ggagcggtat gcagaccctc tgctttacca aatccagaaa
     1261 tatggcagag atcacagcac ttcgctgccg tgagagtctg ccaagcagca gtatctcctc
     1321 ttaccgggga ggctatcgtc cgaacgaacg gagagtcatt gagaatgatc tcaaaaaggg
     1381 agatattgct ggcgttatca gcaccaatgc cctggagctc ggtattgatg ttggcggtct
     1441 tgactcggtg atcatctgcg gctttccggg aaccatgatg tctgttcgtc agcaggcggg
     1501 acgggctgga cgcggaggaa aagatgccct tatcaccttt attgcaaatc tcaacccgct
     1561 agatcagtac ttcatgcggt gtcctgatgc tttctttgac gcaccacatg agcgcccaat
     1621 tcttgatctt gaaaatccct atcttttgcg ggagcatatt ctctgtgccg ctgccgaatt
     1681 accctatcgg acggaacggg atggtgagta cttcggacca cttgccgctg agattgtccg
     1741 tagtctgaaa gaggagcatc tccttgcaag cacacccaaa ggatatgtgt actgcggcac
     1801 cgaacctcct tcccagcagg tatctctttc cggacgaacg tctggaacct gtacgatcat
     1861 gcatgggagc cgtgtgctgg aaacaatgga cacatcacag atgtttcggg aggcctatcc
     1921 gggagcggtt ctctatcatc agagcgagcg ttatcgtgtg gaggaggttg accggaagaa
     1981 tctcattgtg cacgtcaaaa acatcacgga aaattaccat acccgaccat tctataccac
     2041 cgacgtccgt gtcctttcct gcgaaaaaac ctaccgtcac ggttctctcc ttgtttatta
     2101 cggttcggtt tccgtttgtt cacagatgat cggttactct gttcttgagt atgatcagat
     2161 agtttccacc cactcccttg acgttccccc gctatccttc accaccaaag cctgctggtt
     2221 cgttccggac tgcggcaatg agatccatcc atcagagatt gccggatctc tacacggtac
     2281 agaacatgcc ttgattgctg cgatgccggt acatgtcctc tgtgacagat ctgatattgg
     2341 cggtgtatcc aatcccttcc attccgatgt aggg
//
LOCUS       DALUAJ010000176         1538 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_33875,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000176 DALUAJ010000000
VERSION     DALUAJ010000176.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33875"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="O0X37_03750"
     CDS             <1..339
                     /locus_tag="O0X37_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter ATP-binding protein"
                     /protein_id="HJK62070.1"
                     /translation="AVARALATDPAVMLMDEPFGALDAQTRNKMQRELLNIWQETKKT
                     ILFVTHSVDEAVYLSDKIVVLTPRPGTVHEIDDIDLPRPRDRTSVEFAQIRKNILHVI
                     EDLETGNQNI"
     gene            394..894
                     /locus_tag="O0X37_03755"
     CDS             394..894
                     /locus_tag="O0X37_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HJK62071.1"
                     /translation="MVRKPARMYRDLAKKAYCRREYMGGVPGVKVVQFDMGNLSADFP
                     VAIHLEVLEACQIRHTAMEAARINMNRRLMKDVGRNNFHLKIRAYPHHVIREHKQATG
                     AGADRVSEGMRLAFGKAVGTAARVAPRQRIFTVWTTEQYINQAKDALLHSGYKLPTPT
                     RVVVEN"
     gene            1130..>1538
                     /locus_tag="O0X37_03760"
     CDS             1130..>1538
                     /locus_tag="O0X37_03760"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HJK62072.1"
                     /translation="MDSLLFERLAQAVEDAVREAEIILPPDVEAALRRAANRETNPVA
                     RGEFANILDNLSKARDLRVPICQDTGIPVIYLTVPPTVPVSCDLYDAVADGVRRATRS
                     VPLRPNGVDPISRENSGDNTGAGLPPVHIVPGDV"
BASE COUNT          345 a          368 c          406 g          419 t
ORIGIN      
        1 gcggttgcgc gcgcccttgc gacggatccg gctgtcatgc tgatggatga accgttcggt
       61 gctcttgatg cccagacacg caataaaatg cagcgcgagc tgttaaacat ctggcaggag
      121 acaaaaaaga cgattctgtt tgtgactcac agcgttgatg aggcagtgta tctttcggat
      181 aaaatcgtag ttctgacgcc ccgtcccgga accgttcatg agattgacga tattgatctg
      241 cccaggccgc gggatcggac gagtgttgag tttgcccaga ttcggaaaaa cattctgcat
      301 gttattgagg atctggaaac tggcaatcag aacatttaat atcgtttccg tcaaacctga
      361 ttagcacatt tagagagcga ggagttattc aaaatggtca gaaaaccagc acgaatgtac
      421 cgcgaccttg ctaagaaggc atactgccgc cgtgaataca tgggtggtgt gcctggtgtt
      481 aaggttgtac agtttgatat gggaaacctc tctgctgatt tcccggttgc aattcacctt
      541 gaagttcttg aggcatgcca gatccgccac acggcaatgg aagcggcacg tatcaacatg
      601 aaccgccgtc tgatgaagga tgttggaaga aacaacttcc acctgaagat ccgggcatac
      661 ccgcaccacg ttatccgtga gcacaagcag gcaaccggtg ccggtgcaga ccgtgtttct
      721 gagggtatgc gccttgcatt cggtaaggct gtaggtactg ctgcacgtgt tgctccgaga
      781 cagcgcatct tcaccgtctg gacgaccgag cagtatatta accaggcaaa ggacgctctt
      841 ctgcacagcg gatacaagct tccgacgccg acccgtgttg tagttgagaa ctaagcctac
      901 cctggctctc aaccaatact atattttcct tttgcagttt tttgttagtg ttcgtgtctt
      961 tttcgatttg ttcgttgttc gtccggcgtt tttcgtgata tttaccatcg cagaatgtac
     1021 tttctgtctg tttcaaacaa agaacatccc tgatgtttct ttttccgtct ttgtgaaggg
     1081 gtctgttcaa acgacagtct taatccatat ctcctgcaat actcttttca tggattccct
     1141 gctgtttgaa cgtctggccc aggccgtgga agatgcggtg cgcgaagcgg agattattct
     1201 tcctcctgat gttgaggcgg ctcttcggcg tgcagcaaat cgtgagacaa atcctgttgc
     1261 acgtggtgag tttgcaaata ttctggataa tctttcgaag gcgagggatc tgcgtgttcc
     1321 gatctgtcag gatacgggta ttcctgtgat atatctgaca gtgccgccaa ctgtcccggt
     1381 aagttgtgat ctgtatgatg ctgttgctga tggtgtccgg cgggcgacgc ggtctgtgcc
     1441 tctccggcca aatggagttg atcccatctc tcgggaaaac tccggggaca atacgggcgc
     1501 gggtcttcca ccggtacaca ttgttccagg tgatgtac
//
LOCUS       DALUAJ010000177         6023 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_34020,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000177 DALUAJ010000000
VERSION     DALUAJ010000177.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34020"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            108..908
                     /locus_tag="O0X37_03765"
     CDS             108..908
                     /locus_tag="O0X37_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HJK62073.1"
                     /translation="MPDDPLSNILRSKRETTRFQVLVEVAEHQPSIRQQEIAEKLGVT
                     PQAISEYVRDLAEDGFISAEGRGRYYVTHKGVEWVLNNAEVLEAYARHVRRDIIHQVA
                     TWAAIADSDLKTGDSVGVYMKNGWLYAGRQPQTAMGMVIADANAGMDVGVARLAGIID
                     HTEGKVDVAKVPRIERGGSKMISPDKLLPLVKKADIVGAVGLEAYLALKNAKINPDMF
                     FGAREGVIEAAFHGMHCLILIVDEEFTDFLKRLETAGLSYTIHELVSQ"
     gene            909..2924
                     /locus_tag="O0X37_03770"
                     /pseudo
     CDS             909..2924
                     /locus_tag="O0X37_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(3707..3970)
                     /locus_tag="O0X37_03775"
     CDS             complement(3707..3970)
                     /locus_tag="O0X37_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62074.1"
                     /translation="MDSYTIRKIDARRFTVTVTSPSKQSWRTLESAGLSVTDEKRASY
                     KGGECYSWTVQAIQPGIYELKMIQQSDDGAYRKVVIPIIAEAS"
     gene            complement(4200..4283)
                     /locus_tag="O0X37_03780"
     tRNA            complement(4200..4283)
                     /locus_tag="O0X37_03780"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4247..4249),aa:Ser,seq:cga)
     gene            complement(4415..6001)
                     /gene="thsA"
                     /locus_tag="O0X37_03785"
     CDS             complement(4415..6001)
                     /gene="thsA"
                     /locus_tag="O0X37_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thermosome subunit alpha"
                     /protein_id="HJK62075.1"
                     /translation="MSSRSGQPVVILRDNVERVPGQEALRSNIMAAKVLGNTVRTTLG
                     PRGMDKMLVTQGSDDIVITNDGATILHQIHVQHPGAKLVVEVAETQDDECGDGTTTAV
                     VMVGSFMEQAENLIDAGVHPSVIAKGYNLGMMKALEILDTLAIEVTPKDTAMLKQIAK
                     TAMTGKSIEMIMDKACDVVVEAVSNVAVTSGKKTTVNEDDILVKTKRSETMDAEMIKG
                     VLIDKTRLDALMPKKIKGVKAAFIANPLEITKTQTKSKIKITSHEQLEAFSVAERETL
                     RAMAEKFVENGVNVVLCQKGIADPVQYYLAENGIYALEFVPEKDLKYAAKALGGQIVN
                     KPEDLSPDVIGTAGNLEMLEDIEMTKLSGCKNPQAVTILLRGSSQHLVDELERAIEDA
                     TRVVQDVVEDGAYLIGGGSVETELALRLSEYAATEGGRVQLAIEGYAKAFEIIPKTLA
                     ENSGFDTVDKVIDLRQAHATGEKYAGLNVFTGKVVDMKSEGVVEPKRVKRQAIQSASE
                     TAMLLIRVDDMMISKGADQM"
BASE COUNT         1413 a         1514 c         1587 g         1509 t
ORIGIN      
        1 accgcgcacg atgattctgc cgcaacgcat ttactcactc gaaaaccatt atagaattac
       61 aaccaatata agtgtagggt ttctgcacca gaaaccagga agaaactatg cccgacgatc
      121 ccttatccaa tatacttaga agtaagcgtg agaccacacg ctttcaagtc ctcgtagaag
      181 tcgcggaaca tcaaccctcc atccgccagc aggagatcgc cgaaaaactt ggtgtcaccc
      241 ctcaggctat ctccgagtat gtccgtgacc ttgccgaaga cggcttcatt agtgcggagg
      301 gacgcggtcg ttactacgtc acacacaaag gcgttgagtg ggtcttaaac aacgctgagg
      361 tccttgaggc ctatgctcgg catgtccgca gggatattat tcaccaggtt gcaacttggg
      421 ctgcaattgc cgattctgac cttaaaacgg gggactctgt cggtgtgtat atgaaaaacg
      481 gatggctgta tgcaggccgt cagccgcaga cagcaatggg catggtaatt gcggacgcaa
      541 atgcagggat ggatgtcggc gttgcccgtc ttgcgggtat catcgaccac actgagggca
      601 aagtggacgt cgctaaagta ccgcggatcg aacgcggcgg ttcgaagatg atctcgccgg
      661 ataaacttct tcctctggtg aaaaaagcag atatagtcgg tgctgtcggt cttgaagctt
      721 accttgcact gaaaaatgcc aagatcaatc ctgacatgtt ttttggtgca cgggaaggtg
      781 tcattgaagc agcgttccac gggatgcact gcctgatcct tattgtggat gaagagttca
      841 cagacttcct taaaagactt gagaccgcag gtctctccta tacgatacat gaactggtga
      901 gtcaataagt ggtgaccgtt gtccaaccct ataaaaaagg gtacaaagtc tatttttgtg
      961 acgattcaaa aaatggaaaa ataaaacacg tcggaactgt tgaactggag gagacctcta
     1021 agggtatccg cccttcagag ttcttcgtac ggcgtcccgg gacatctcat gcccagaaaa
     1081 ctccgacaaa ggagcttatc acagtccttc gtgcaaacgg tatggtacag ctgaccgaga
     1141 cattgccgga gtttcaggat ttcctgcgaa gtatgcagat cccctgggaa aaggtgacgc
     1201 tctgccggac ctgcatgttt gatgatcggc ttacgccgct gtcgggggca actcgtatca
     1261 aatgcggtca ggagtatgtc tgtctggact gtgcaaagcg tgagctccgc cgcgagcttg
     1321 cgcacatcaa acggcttggc aaacggaccc agaaccacat cgaggatctg ctggaggagt
     1381 accgcgatct tgatatggta ctggcaattc ttcagccgga gtcgcttgac acgaagaaaa
     1441 caatgttcga ccggctggaa gcacacgaac agacgccgac ggaacgtatc gagaaccttc
     1501 cgctgccgcg tgagtttgtg gatatctgcg gggtcgagac ccttatgcct gcccagcagc
     1561 gtgctgttga ggcaggacta ttgtttggaa aggatctttt ggtggttgcg gcaactgctt
     1621 caggaaagac gttcatcggc gagatggctg gtttgaaaaa ttatctggaa aagcgtggtc
     1681 gtatgctctt ccttgtgccg ctggttgctc tagcaaacca gaagtacgat cgtttttcca
     1741 gaaaatatga gagtgttgta acaacctcgg taatgacggg tatctcccgc atcaatcttc
     1801 cggagacaag gcctgtcggt aaccgcgggg cgggtggcgg cattgtggtc ggaacgtatg
     1861 agggtgtgga caatcttctc cgcaaagggt ccggtcttca caacatcggt acaatcgtta
     1921 tcgatgaggt acagatgctc gaggatccgg aacgcgggca tcgtcttgac ggccttattg
     1981 cccgactgaa gtatgctgcc cccaaagctc agtttctgta tctttcggca acgattggat
     2041 cccctcatct gcttgcgaaa aagcttggag ccaccctggt ctcgtatgct gatcggccgg
     2101 tcgctcttga acgacatctc atcttcacag aaaaggatgc gaaggttccc tacattaaga
     2161 aactcgtgtt tgaggagtac aataaaactt catcaaaagg gtaccgcggt cagagtatca
     2221 tcttcacgaa tgcccgtaca cgttgccata cgatagctga tgccattgga aagcttgctg
     2281 ccccgtacca tgccggtctt acttcgcagg aacggcgtgc agttgaaaaa cagttcgagg
     2341 atggtaaact tgcctgtgtt gtaacaactg cggcgcttgc agcgggtgtg gacttccctg
     2401 catcacaggt gatctttgac gcacttgcaa tggggatcaa ctggctgaca gtacaggagt
     2461 tccatcagat gatgggccgt gcaggtcgtc ctgattttca tgatattggc cgcgtggttg
     2521 ttcttgcaga gccgggagca tcgtattcac gcgagacgaa actgactgag gaagaggttg
     2581 ccatccgcct tctcaaaggc gacatggaag aagttgctcc tgtctacgac attgaagaga
     2641 cctcggaaga gtttgcggcg aacgcgatcg tgtgccgcgg acgtgaagca gatattgtga
     2701 aggtcggtga gagtatggtg ggttgtggtg aggatccgct gccggaactt ttgcgtcaca
     2761 aactcgtccg gaagaacgga ggagttctgg aactctctcc gcttggacgt gtgatggccg
     2821 agcagttcat cggcatggag aaactccttg agattgatcg gctcgttcgg attatggatg
     2881 atccgctgga actgatcgca gagattgagt gtgccgaaga ggagcgtgat aaagaggcac
     2941 gacgtgccga tcgcaggatg accgagaaga aggagtccga ctggatggct ggaaagaaaa
     3001 cttcgtctgc cccgtcatca gagaagaaga tacgcaggga tgctcctcct gcctgggttg
     3061 ccgaacggaa gtctgcgatc gttgcggcga aacgtgaaca tgagagtgat cgtaaactcc
     3121 gttccggcaa aactgaaccg gagcgaaaac tccggcgtga acatgaagtc ggcaccaccg
     3181 aggtaaaagc accgcgtcgt gcaagcaagg aactagagga tcggatcgag cgtaagaagc
     3241 gtgaggcggc atcatttatc cctgctccaa aacgaaagaa gactgatgag tgggatgcaa
     3301 ttgaagcagg tattgatcag cgccgtgccg gaaaatatga cgatgcactg aagatcctcg
     3361 gcagttacgt tgcggatcat ccttctgaca gtcgtgcact gaccgagctt ggcaaggtgt
     3421 atgatctccg cggtgatcgc gatacggctt actcctgcta tcagcgtgca gcagcggcag
     3481 acactcggaa ccgcgaggcg attgatcgga tgaacgccta tcttattgga attaccgtgg
     3541 acattgatgc ggacgctcct gccaaacgtc agccgactgg tgtggtgaaa aagaagaaac
     3601 agtgaactcc cctgatattt tttttatttt ctattttccg gtccgcacgg gtcccgtaaa
     3661 atgtttgtca caatcaaaaa agatcgggtg aatgccccga tatgcatcag gaagcttccg
     3721 caatgattgg gataaccact ttgcggtatg ccccgtcatc actctgctga atcattttta
     3781 gttcataaat gcctggttgt atcgcctgaa ccgtccagga ataacactcg ccacccttgt
     3841 acgacgcgcg tttctcatcc gtcactgaaa gtcccgcgga ctccagtgtc cgccaggact
     3901 gtttgcttgg gctcgttacg gttaccgtaa accttcgcgc gtcgattttt cggatcgtat
     3961 atgagtccat gttctctttc tccgttctgg attgggtttt tctgataact ctgcggtttc
     4021 gcaattgatg atatattgtt atcctatata aatgatgtcg tcaaattttg cgcataattt
     4081 tgcaaagtcc ctatctgttt tccttgttat tatgtcgaat ttctgttcga cggtaactga
     4141 tgctgacaca ggaatatgac aggttcccga tgtactcgta gtattttgta aaataacgac
     4201 gccgaggggg agatttgaac tcccgaggtg ttaccaccag aggctttcga ggccaccgcc
     4261 cttccggact agactacctc ggcacatccg tttagcactc aaaacagtat gcgcgaccag
     4321 ttcttcgcca atgacagcaa gacatgatct ctcgcaacac tttactgcct atacacatcg
     4381 acttgtggtc taaaaaagat agtgtttttt ggtattacat ctggtcggcg cctttgctga
     4441 tcatcatgtc atccacacgg atcagcagca ttgccgtctc ggatgcgctc tggatcgcct
     4501 ggcgttttac acgcttcggc tccacaacgc cttccgactt catgtcaaca acctttccgg
     4561 taaacacgtt cagacctgca tacttctcgc cggttgcgtg tgcctggcgg agatcaatga
     4621 ccttgtcaac tgtatcaaaa cctgagtttt ctgcaagtgt cttggggatg atctcaaatg
     4681 cctttgcgta tccctcgatt gcaagctgta cacggccgcc ctcggttgct gcgtactcac
     4741 tgagacgcag ggcgagttct gtctcaacgg atccgccgcc gatcaggtat gctccgtctt
     4801 caacgacatc ctgaaccaca cgggttgcat cctcaattgc acgctccagt tcgtccacga
     4861 ggtgctggga agatccgcgc agaagaatag ttaccgcctg cgggttcttg catccggaga
     4921 gcttggtcat ctcaatgtcc tcaagcatct caaggtttcc tgcggttccg atcacatcgg
     4981 ggctgagatc ctctggcttg ttgacgatct gtccgccgag tgcctttgca gcatacttca
     5041 gatccttctc cggcacaaac tcaagtgcat agataccgtt ctcggcaagg tagtactgca
     5101 ccggatctgc aattcccttc tggcagagga ctacattgac accgttttcc acaaacttct
     5161 cggccattgc gcggagtgtc tcacgctctg caacggagaa tgcctcaagc tgctcgtggg
     5221 aagtgatctt gatctttgat ttggtctggg tcttggtgat ctcaagcggg tttgcaatga
     5281 atgcagcctt tacgccctta atcttcttcg gcatcagtgc gtcaagtctg gtcttgtcaa
     5341 ttaagacacc tttgatcatc tcagcgtcca tcgtctcgct gcgctttgtc ttgacaagaa
     5401 tgtcgtcctc gttgaccgtc gtcttcttgc cggaggtaac cgcaacattg ctgaccgcct
     5461 ccacaacaac atcacaggcc ttgtccataa tcatctcaat ggacttaccg gtcattgctg
     5521 tctttgcaat ctgcttgagc attgcggtgt ctttcggagt aacttcgatt gcgagggtat
     5581 cgaggatctc aagtgccttc atcataccaa ggttgtaccc ttttgcgata actgacgggt
     5641 gaacacctgc gtcgataagg ttctcggcct gctccatgaa ggatcctacc atgacaaccg
     5701 cggttgtggt tccgtcaccg cactcgtcat cctgtgtctc tgcgacctca acaacgagtt
     5761 ttgcaccggg gtgctgtacg tgaatctggt gaaggatggt tgcgccgtca ttggtgatga
     5821 cgatgtcatc gcttccctgc gtaaccagca tcttgtccat accgcggggg ccaagagtgg
     5881 tccgaacagt gtttccaagg acctttgcgg ccatgatatt ggagcggagc gcctcctgtc
     5941 caggcacgcg ctcaacgttg tcccgtaaaa taacgacagg ttgtcctgat ctgcttgaca
     6001 tgaaatctat tatctcctgt act
//
LOCUS       DALUAJ010000178         1525 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_34221,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000178 DALUAJ010000000
VERSION     DALUAJ010000178.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34221"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(44..>1525)
                     /locus_tag="O0X37_03790"
     CDS             complement(44..>1525)
                     /locus_tag="O0X37_03790"
                     /inference="COORDINATES: protein motif:HMM:NF036590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N C-terminal domain-containing
                     protein"
                     /protein_id="HJK62076.1"
                     /translation="VKAPEFTRMIQTILGKRAFSWALDLYHRKFAGRNASPNDWLETM
                     GSIGKIDFSTMAGRWLTQTGYPTVTATASYNPEEQTAEITVEQTGFGDQDPWIFPLVG
                     TLLTDKGNTVAEIFEKIDGAHQTFSVPCAGAFAAASWNPGHAAYIRINSTASDDELYL
                     LLKYDTDTVSRFLAWQTLVDREMYKLCSNPAAVTDPRLVNWYIETLTNNTKMQSTGVL
                     PLTIFEYTSDPAYSYCYTTLHAAKQRILRSVSETLGKTRLNVLYTTYNLPERQTTTPA
                     ELARIFKARAVKNLILTHLAAFDTPDVHEILKTACQTATCATDRTHALTLYLSSSAPD
                     RLDLLKTELTRAKADPVAWENFLAAVAASSSPDTVRYLRYIEQRGNLHIEQAGESRSL
                     YLRFAANRKISLETEEGREFLSDSLTTLARVNEYISTGILNVFSHLDSYEDTVRDACF
                     AILTNLINTVPETEAPSVIRTARRILQKSPKAKEAYDQAHRST"
BASE COUNT          309 a          332 c          483 g          401 t
ORIGIN      
        1 ctttcatatt tatacgggca agccatatgc ggcgttctgc tgcttacgta ctccggtgcg
       61 cctgatcata tgcttctttg gctttcggac ttttctgaag aatgcggcgt gcggtacgga
      121 tgacggacgg agcctcggtc tcgggaacgg tgtttatcag gttggtaaga atggcaaaac
      181 acgcatcacg gacggtgtct tcatagctgt caagatggga gaagacgttg agaatgccgg
      241 ttgagatgta ctcgttaacc cgggcaaggg tggtgagaga gtctgaaaga aactcgcgtc
      301 cctcttctgt ttcaagggag atcttgcggt ttgcagcaaa ccggaggtag agggatctgc
      361 tttctcctgc ctgttcaatg tggaggtttc cacgctgttc gatgtatctg agatagcgta
      421 ctgtatctgg cgaggaggac gcggcaacgg ctgcaaggaa gttctcccag gcaaccgggt
      481 cggcttttgc ccgggtgagt tctgtcttca ggagatccaa cctgtcaggt gcggagcttg
      541 agaggtacag cgtgagggca tgcgtccgat ctgtagcgca ggtcgcggtt tggcaggctg
      601 ttttgagaat ctcgtgaaca tcaggtgtgt caaaggctgc gagatgggta aggatcagat
      661 tcttcactgc ccgtgctttg aagatacgtg caagttccgc cggtgttgtg gtttgacgtt
      721 ccgggaggtt gtaggtggtg tagagtacat tgaggcgtgt ctttcccagg gtttcggaca
      781 ccgatcgtag gatacgctgt tttgctgcgt ggagggtggt ataacagtag gagtaggcgg
      841 gatcgcttgt gtactcgaag atggtcaggg gaagaacacc ggtactttgc attttggtat
      901 tattggtcaa tgtctcaatg taccagttga caagacgcgg atcagtgact gccgcaggat
      961 tgctgcagag tttgtacatc tcacgatcta ccagtgtctg ccatgcaagg aatctgctga
     1021 cggtatctgt atcgtacttg agcaggaggt acagttcgtc gtctgatgcg gtgctgttga
     1081 tgcggatgta ggcagcgtgg ccggggttcc aggatgcagc agcaaaagct cctgcacacg
     1141 ggacagaaaa tgtctggtgc gccccgtcga ttttttcaaa gatctctgca acggtgtttc
     1201 ctttgtcggt gagcagggtt cctacgagag ggaagatcca gggatcctga tccccgaatc
     1261 cggtctgttc aactgtaatc tcggcagttt gttcctcggg gttgtatgag gccgttgcgg
     1321 tgacggttgg gtagccggtc tgtgtgagcc agcggcctgc catggtggag aagtcaattt
     1381 ttccgatgga ccccatggtt tcaagccagt cgttcgggga tgcattcctg cccgcaaact
     1441 tacggtgata cagatcaagt gcccaggaga aagcccgctt tcccagtatt gtctggatca
     1501 tgcgggtaaa ctcaggggct ttgac
//
LOCUS       DALUAJ010000179         2512 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_34602,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000179 DALUAJ010000000
VERSION     DALUAJ010000179.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34602"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="O0X37_03795"
     CDS             complement(<1..462)
                     /locus_tag="O0X37_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HJK62077.1"
                     /translation="MRQEIRFSGLGGQGIITAAVILGRAAALYGGKHVVQTQSYGPEA
                     RGGASASAVIISDEPIHYPKVTDPHIYAIMSEEAFKKYGANAAKDAIMLIDPGYVTSR
                     PDCTCYMVPATAVAKERLGKPVFANVIMLGGLLEATGIISYEALEHAVLDSV"
     gene            complement(459..1247)
                     /locus_tag="O0X37_03800"
     CDS             complement(459..1247)
                     /locus_tag="O0X37_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HJK62078.1"
                     /translation="MTLEDWYRQDRLPHIYCAGCGNGTILNCTLRAVNELGLDRDKTT
                     FVSGIGCSSRAAGYTSCDSLHTTHGRALPFATGVKLVKPENNVIVFTGDGDLSAIGGN
                     HFIHACRRNIDLTVVCMNNSIYGMTGGQGSPCTPYGAITTTTPYGMHEMPFDLCAVAE
                     AAGANYVARWTSFHVKELTAAIRAGIETPGLSFIEVMAQCPTSFGSKNKMKQVSQMID
                     MFRTNAVLKGKADRDAAAGIPLAPGKFVVGELVRRSNPVLGGRP"
     gene            complement(1244..2377)
                     /locus_tag="O0X37_03805"
     CDS             complement(1244..2377)
                     /locus_tag="O0X37_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HJK62079.1"
                     /translation="MTDKIEFLNGNIACAEGAIAAGCRFFAGYPITPSTEVAERMAAR
                     LPKVGGTFIQMEDELASMAAIIGGAWAGARTMTATSGPGFSLMMENIGYAAMTETPCV
                     VVNVQRGGPSTGQPTMAAQGDMMQARFGSHGDYSIIALSPSSVQEMFDLTVKAFNLAD
                     RFRTPVFLMADETIGHMREKVVLRDNVEITPRRELSTGDLPCQPDVEGVPGFGTFGNG
                     HGVHITGLTHDERGYPATDSAALHESLVRRQVGKIENFRNEIADVDIVNPDAEIVFIS
                     YGAPTRAVQQLLADYPGVYGHLNLRIAWPFPDAKLSEFHAVKAFVVPEMNLGQIAREV
                     RMHTNVPVVTAPKLGGALHTVDELKHAADVAETATGTFTEVLL"
     gene            complement(2374..>2512)
                     /locus_tag="O0X37_03810"
     CDS             complement(2374..>2512)
                     /locus_tag="O0X37_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HJK62080.1"
                     /translation="RCTNCRLQIMYGRQLCGMCQMTCPEQAISWVEEKPYEAHKVVIE
                     Y"
BASE COUNT          585 a          669 c          672 g          586 t
ORIGIN      
        1 aacgctgtca agaacagcat gctcaagcgc ttcatagctg atgatgccgg tagcctccag
       61 aaggccgcca agcatgatga cgttggcaaa caccggcttt ccaagccgtt cctttgcaac
      121 ggctgttgcc gggaccatat aacaggtaca gtccgggcgg ctggtaacat acccaggatc
      181 aatcagcatg atcgcatctt ttgcggcgtt tgcaccgtac ttcttgaacg cttcttcgga
      241 cattatcgca tagatgtgtg gatcagtgac ctttggataa tgaatcggct catcggagat
      301 gatgacagcg gatgcacttg caccaccacg cgcttcgggt ccgtagcttt gtgtctggac
      361 aacatgtttt cccccataga gtgctgcagc acgaccgaga atgacagctg cagtaatgat
      421 accctgacct ccgagcccag agaatcgtat ctcctgcctc atggtctacc tcctagaacc
      481 gggttgctgc gccggacaag ttcaccaaca acgaactttc ccggagcaag cggaatgccg
      541 gcagccgcat cacgatcggc ttttcctttc agaaccgcat tcgtacggaa catgtcgatc
      601 atctgagaga cctgcttcat tttgttttta ctgccaaagg acgtggggca ctgtgccatg
      661 acctcaatga aggagagacc gggtgtttca attcctgcac ggattgctgc agtgagctcc
      721 ttgacgtgga acgaggtcca gcgtgcaaca tagtttgcac cggctgcttc ggcaacagca
      781 caaagatcaa acggcatttc atgcataccg tagggagtgg tagtagtgat tgcaccgtac
      841 ggagtgcagg ggcttccctg acctccggtc atcccgtaga tactgttgtt catgcagaca
      901 acggtgagat caatgtttct ccggcatgca tgaatgaaat ggttaccacc tattgcggaa
      961 agatcaccgt ccccagtaaa gacgatgacg ttattttccg gttttacaag tttaacaccg
     1021 gttgcaaacg gcagtgcacg accgtgcgta gtgtgcaggg agtcacaaga ggtgtatccg
     1081 gcagcacgtg aagaacaacc aattccagag acaaaagttg ttttgtcgcg gtccagccca
     1141 agttcgttta ctgcccggag tgtgcagtta aggatggttc cgttcccgca acctgcacag
     1201 tagatgtgag gtaggcgatc ttggcggtac cagtcttcga gggtcacagc agcacctccg
     1261 tgaatgtacc agtagcggtt tccgcaacgt ctgcggcgtg cttaagctcg tcaacggtgt
     1321 gcagtgcacc accgagtttg ggggctgtga caaccgggac gttggtatgc atacggacct
     1381 cgcgagctat ctggccgagg ttcatttcgg gaacgacaaa tgctttcact gcatggaact
     1441 cggaaagttt tgcgtccggg aacggccagg caattctaag atttaagtgg ccgtatactc
     1501 ccggatagtc agccagaagc tgctggactg ctctggttgg tgctccatag cttatgaaaa
     1561 caatttctgc atcagggttt acgatgtcaa catctgcgat ctcatttcgg aagttctcaa
     1621 ttttcccgac ctgacgtctg acaaggctct cgtgcagggc tgcggagtcg gttgccggat
     1681 atccccgctc gtcatgggtg agtccggtga tgtgaacacc gtgtccgttt ccgaaggtac
     1741 cgaaacctgg aactccctcc acgtccggct ggcagggaag atcacctgta gagagttcac
     1801 gacgtggagt aatctcgacg ttgtcccgta aaacaacctt ttcccgcatg tgaccaattg
     1861 tttcatccgc catgagaaaa acgggggtcc ggaatcggtc tgcaagatta aaggctttga
     1921 cagtcagatc aaacatctcc tgcacagagg atggagaaag ggcaatgatg ctgtaatctc
     1981 catgagagcc gaaccgggcc tgcatcatat ctccctgagc cgccattgtg ggctgacccg
     2041 tggatgggcc tccacgctga acattgacaa ccacgcaggg agtctcggtc atggcagcat
     2101 aacctatgtt ttccatcatg agagagaaac cagggcctga ggttgcagtc atcgtacgtg
     2161 cgcctgccca agcgcctcca atgattgcag ccatactggc aagctcatcc tccatctgga
     2221 tgaaggtccc gccgactttg ggaaggcggg ctgccattcg ctcagcaacc tccgtggacg
     2281 gagtaatcgg gtatccggca aagaagcggc acccagcagc aattgcaccc tcagcacagg
     2341 cgatatttcc attgaggaat tcgattttat cagtcaatat tcaatcacca ctttgtgtgc
     2401 ctcgtagggt ttttcctcta cccatgagat ggcctgctcc ggacaggtca tctgacacat
     2461 cccgcagagc tggcgaccat acattatctg caggcggcag ttggtgcagc gc
//
LOCUS       DALUAJ010000180         3533 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_34674,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000180 DALUAJ010000000
VERSION     DALUAJ010000180.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34674"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="O0X37_03815"
     CDS             <1..890
                     /locus_tag="O0X37_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJK62081.1"
                     /translation="KAREEIPLVEDTVASLAGKIGALTAKSETYREAVDLVVAYAPEE
                     IEFSLDDLVCTPDQLAIVRKVQISLDNQEFLRSHRIYELGRILLVGPPGTGKTSFALA
                     LSRSVHMPVLEVRLSMLTSQYLGETSKNIDRIFDLAKKIAPCILFIDEFDYIAKTRIS
                     DDNGTMKRAVNTLLKSIDHINLIKDQVLLIGATNHAGMLDEAAWRRFDEVVTFTLPDT
                     TMREAILRHVASDIPCTMDFTTLAERTDGFSGADLRMMLTEAIVSALLAGRKEINEED
                     VNAGMELVNRRNIARSGCL"
     gene            896..1630
                     /locus_tag="O0X37_03820"
     CDS             896..1630
                     /locus_tag="O0X37_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJK62082.1"
                     /translation="MKITALGTGDVVGTPKIGCDCEVCREARRTGKHRLRTSILVEHK
                     GKHLLIDTGPDMRAQLLAAGSPYIDGVIWTHGHYDHFMGFGDFYRVQRELIPVYGAAE
                     VLNYCGGIFSFMRHREVTMIPYEPMEICGMLVTIFPVMHDTPTYGVRIEADGAVFVYS
                     CDTTDQLSARTLDTMKGADLLLLDGIFPPEVHISKHMNIREAELLAEKLSPKEFWCVH
                     MSHKIWWSYRYGAQDMQTWDLPKNSE"
     gene            1694..2698
                     /locus_tag="O0X37_03825"
     CDS             1694..2698
                     /locus_tag="O0X37_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HJK62083.1"
                     /translation="MKKDIYYRVDKRVQNGVVKYYLMREVNSDTRKFRASKLIKAGTP
                     PTDPEVARAILLYGFELEMKCCEKVVSYRVKNFKYQYEENEKIYSQLEKYRYLDLRYQ
                     DYLSEEEAARRISEREYHYIHESVKNVGCTFTYPEVVQMLETGKIPNGKYLRDINEIQ
                     NLYRCAVLRNKNPRKVTLALVLQIRNMILDNIEEPEQISPEKKAAIQKLLNTYYQKIN
                     DGYHPAEQIIILYNNFRKLKPFKNGSKRTLREIIQYLVLPIGYPLEYQSSQLIVQASE
                     MLTQHQTKISIPNIIEQYAEAGKISHLEEQIRKKIVERWDLQKNKNQKQLDFYLSDDC
                     "
     gene            2757..3107
                     /locus_tag="O0X37_03830"
     CDS             2757..3107
                     /locus_tag="O0X37_03830"
                     /inference="COORDINATES: protein motif:HMM:NF024088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HJK62084.1"
                     /translation="MINKLSFMGLVGLLGLLSIPTGDVRYCAFFAFFVFFGYLFTIED
                     ELFTAYVRKAASTAFFLQTALFFIIYAGTVLIGDAAVFKAAFPLTFAAGMFVFICLLV
                     IHEYREQRGVESGT"
     gene            3097..3300
                     /locus_tag="O0X37_03835"
     CDS             3097..3300
                     /locus_tag="O0X37_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HJK62085.1"
                     /translation="MALKTKIREYRVKQNITQEDLAKAVGVRRETIGHLENGKYNPSL
                     KLGFDIAKVLNVPVEELFEFTDD"
     gene            complement(3346..>3533)
                     /locus_tag="O0X37_03840"
     CDS             complement(3346..>3533)
                     /locus_tag="O0X37_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62086.1"
                     /translation="FTVMNVGCVLGVALFSVAAATGSGSADVYTPTGIAIACAVGFVV
                     SVIALVTSKLARDTVIC"
BASE COUNT         1052 a          858 c          821 g          802 t
ORIGIN      
        1 aaaaagcacg tgaagaaata ccgctcgttg aagacaccgt ggcatccctt gccggaaaga
       61 taggtgcact tactgcaaag tctgagacat accgtgaagc agtggatctg gttgtcgcat
      121 acgctcccga agagatcgag ttttccctcg atgatctcgt atgtacgccg gaccagcttg
      181 caatcgtccg aaaggttcag atatccttgg ataaccagga atttttacgc agtcacagaa
      241 tttacgagct ggggagaata ctcctcgttg gacctccggg aacaggaaag acctccttcg
      301 cacttgcact ctcgcgttcg gtgcatatgc cggtactgga ggtcaggctc tccatgctga
      361 catcccagta cctgggagag acatccaaaa atatcgatag aatatttgat ctcgcgaaaa
      421 aaattgctcc ctgtattctc ttcattgatg agtttgacta catcgcaaag acccgtatct
      481 ccgacgacaa cggaacaatg aagcgtgctg taaacacact tctcaaaagt atcgatcaca
      541 tcaacctgat caaggatcag gtacttttaa tcggagcaac aaaccacgcg ggaatgctgg
      601 atgaggcggc atggcggaga tttgatgagg tggttacgtt cactctgcct gataccacca
      661 tgcgcgaagc aattctccgg cacgttgcat ccgatatccc ctgcacaatg gatttcacaa
      721 cacttgcaga acggacagac ggattctccg gagctgatct ccgcatgatg ctaacagaag
      781 caatcgtctc agcacttctt gcaggccgca aagagatcaa tgaagaggat gtgaacgctg
      841 gaatggagct ggtaaaccgc cggaacattg cccggagcgg ctgcctgtaa aaaggatgaa
      901 gataacagca cttggtactg gcgacgtggt cggaacacct aagatcggct gtgactgtga
      961 ggtctgccgc gaggcacgca ggaccggaaa acaccggctt cgtacctcga ttcttgttga
     1021 gcacaaaggc aagcatctcc tgatcgacac gggaccggat atgcgtgcac aacttcttgc
     1081 cgcaggatcc ccatacatcg acggagtcat ttggacacac ggccactacg atcacttcat
     1141 ggggttcggt gatttctacc gggtgcagcg tgaactaatt cctgtctacg gtgcagcaga
     1201 agtactcaac tactgtggag gcatcttttc atttatgcgc catcgtgagg tgacgatgat
     1261 accgtatgaa ccaatggaga tctgcgggat gttggtgacg atttttccgg taatgcacga
     1321 tacaccgaca tatggggtac ggattgaagc ggacggggcg gtgtttgtgt actcctgcga
     1381 cacaaccgat cagttgtctg cacgaacact ggatacgatg aaaggtgcag accttctgct
     1441 gcttgacgga atttttccgc cagaggtgca catctccaaa cacatgaaca tccgcgaagc
     1501 tgaactactg gcagagaaac tgtcaccaaa ggagttctgg tgcgtacaca tgagccacaa
     1561 gatctggtgg agttacaggt acggcgcaca ggacatgcag acgtgggatc tcccgaaaaa
     1621 cagtgaataa ttttttttga agtacaccgg cgaaacttac tacacaccct tccgccaata
     1681 tactacacaa aggatgaaga aggacatcta ctatcgtgtg gacaaacgag tgcagaacgg
     1741 tgtggtaaaa tattatctga tgcgtgaggt caacagtgac acgcgcaaat tccgcgcatc
     1801 caaactcatc aaagccggaa ctcctcctac cgacccggaa gttgcgcggg caattcttct
     1861 ctacgggttt gagcttgaga tgaaatgctg tgaaaaagtc gtttcatacc gagtaaagaa
     1921 tttcaaatac cagtacgaag aaaatgaaaa aatctactcc cagctggaaa aatacagata
     1981 tcttgacctg cgctatcagg actacctttc cgaggaggaa gcggcaagac ggataagtga
     2041 gcgtgaatac cactacattc atgaatcggt aaaaaatgtt ggatgcacgt ttacgtatcc
     2101 agaagttgta cagatgcttg aaacaggaaa aataccgaat ggaaaatacc tgcgcgatat
     2161 aaatgagatt caaaatctgt accgctgcgc cgttcttcgc aacaaaaacc cccggaaagt
     2221 cacacttgcc cttgtccttc agatcagaaa catgatcctc gacaacattg aggagccgga
     2281 acagatcagc cctgaaaaaa aggctgccat tcaaaaactt ctcaacacct attatcagaa
     2341 aatcaatgac ggataccatc ccgcagaaca gattatcatc ctgtacaata acttccggaa
     2401 actcaaacca ttcaaaaacg gatccaaacg aacgcttcgg gagatcatac agtacctggt
     2461 tctgccaatc ggctacccgc tggagtatca gtcgagccag ctgatcgtgc aggcgtctga
     2521 gatgttaacg caacaccaga caaagatcag catccccaac attattgaac agtatgccga
     2581 ggcaggaaag atctcgcatc tggaagaaca gatacggaaa aaaatagttg aacggtggga
     2641 tttgcagaaa aacaaaaacc agaaacagct tgacttctat ctatccgacg actgctgatg
     2701 cccattcgat gatgtgacat atttatatca tcatgttaca aatatatcac acaccaatga
     2761 taaacaaact cagtttcatg ggactggttg gattgctcgg actcctttcg ataccaaccg
     2821 gtgatgtacg gtactgtgcc ttctttgcat tcttcgtgtt tttcggctac ctattcacca
     2881 ttgaggatga actattcaca gcatatgtaa gaaaagcagc atcaacagcg tttttcctcc
     2941 agaccgcctt attcttcatc atatatgccg gaacagtact tattggagat gcagcagtgt
     3001 tcaaagccgc atttccgctg acatttgctg caggcatgtt tgtattcatc tgtcttctgg
     3061 taatccacga atacagagag cagcggggcg tggaaagtgg cacttaaaac aaagattcgt
     3121 gagtatcgtg taaaacaaaa tatcacccag gaagatcttg ccaaagccgt aggtgtcagg
     3181 cgtgagacca tcggtcatct ggaaaacgga aagtacaacc catctctgaa acttgggttt
     3241 gatattgcaa aagttctgaa cgtacccgtt gaggaactct ttgagttcac cgacgactaa
     3301 cactcttttt tgataaaaac atcgtcccat aaaaggacag atagatcagc atatcaccgt
     3361 atcacgcgca agttttgagg taacaagtgc gatcaccgaa acaacaaacc caactgcaca
     3421 ggcgatcgca ataccggttg gcgtatacac atcagcactg cctgaaccgg tagcggcagc
     3481 aaccgagaac agggcaactc ccagtacaca tcccacattc atcaccgtaa aga
//
LOCUS       DALUAJ010000181         2164 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_35526,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000181 DALUAJ010000000
VERSION     DALUAJ010000181.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35526"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="O0X37_03845"
     CDS             <1..485
                     /locus_tag="O0X37_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HJK62087.1"
                     /translation="IDGRGGATGSSPKFLKDASSVPTVYALARAAKFLQNHAPEIDLV
                     ITGGLRTSRDVVKALAMGADAVAVASAALMALGCDRYRVCHTGKCPAGIATQDPKLRQ
                     NLSIDESARRVANYLAVTTEELRSFCRVTGHADIHELSAEDLATTSSEISGHTDIRHT
                     "
     gene            complement(493..1722)
                     /locus_tag="O0X37_03850"
     CDS             complement(493..1722)
                     /locus_tag="O0X37_03850"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJK62088.1"
                     /translation="MTPIYPRERYLSKVRPHINKPLIKVITGIRRSGKSALLSLIEDE
                     LRNRGVASDHILRINFEDTAAEPLTDRHRLSSYLEERMTGSPPYYILLDEIQEVGEWE
                     KLVNSLLAGGNTDIYLTGSNSRLLSSELSTYLAGRYIEITVQTLSFSEYLEFIREITG
                     TCPENRQESFMHYLRLGGFPLIHTAQYDETSANIVVGALYSSVVLRDVIQRHNIRSTD
                     LLERLIRYLFDNVGRIFSAKNISDFLKSENRTFSTNTIYEYLGYLEEAFVIHRTPRYD
                     LSGKRILKTLEKYYVSDISLVNATLGYRETAIAGLLENVIFLELLSRDYRVYIGKQGE
                     REIDFIAEKQGEKLYVQVCRTLAGSPETLRREITPLLAAKDQYPKYIVTMDEPWAANQ
                     DGVQILSMAEFLLREHW"
     gene            1982..>2164
                     /locus_tag="O0X37_03855"
     CDS             1982..>2164
                     /locus_tag="O0X37_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833579.1"
                     /note="replaces guanine with 7-cyano-7-deazaguanine at
                     position 15 in the dihydrouridine loop of archaeal tRNAs;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine(15) transglycosylase"
                     /protein_id="HJK62089.1"
                     /translation="MGIYFEVIHKDIAGRVGKLKVGDKTIRTPALLPVVNPHLQIIPP
                     SEMKKMGVEGIITNAYI"
BASE COUNT          527 a          494 c          594 g          549 t
ORIGIN      
        1 cgatcgacgg ccgcggcgga gcaacgggtt cgtcgccgaa gtttttgaag gatgcatcaa
       61 gcgttccgac ggtgtatgcc cttgcacgtg cggcgaagtt tttgcagaac catgcacccg
      121 agatcgatct ggtgattaca gggggactgc ggacgagccg cgatgtggta aaagcactgg
      181 caatgggagc ggacgcggtg gcggttgcat cagccgccct gatggctctc ggctgtgacc
      241 ggtaccgggt ctgccacaca gggaagtgcc cggcaggaat tgcaacacag gatccaaagc
      301 tgcggcagaa cctttcgatc gatgagagtg cccgccgggt tgcaaattat cttgcggtta
      361 ccaccgagga gctgcggtca ttctgccggg taacgggcca tgcggacatt catgaacttt
      421 cagcagagga tcttgccacg accagcagcg agatatccgg tcatacggat atccgccaca
      481 cctgaatttt tcttaccagt gttcccggag aaggaattcg gccatactga ggatctgtac
      541 gccgtcctgg tttgcggccc atggttcatc catagttaca atgtactttg gatactgatc
      601 ttttgcggca agcagcggtg ttatctcgcg tcgaagggtc tcgggagatc ctgcaagcgt
      661 tctgcatacc tgcacgtaca gtttctctcc ctgtttttct gcgatgaaat cgatctcacg
      721 ctctccttgt ttaccgatgt acacacgata gtcccgggag aggagttcaa ggaaaatgac
      781 gttttccaga agtccggcga tcgcggtttc ccggtatccg agggtggcat ttaccagaga
      841 gatgtcgctg acatagtact tttctagcgt tttgagaatc cgttttccgc tcagatcata
      901 tcttggtgtc cggtgaataa cgaaggcctc ttccagataa ccgaggtact cgtatatggt
      961 gtttgttgag aatgtgcgat tttcactttt aagaaaatcc gagatgtttt ttgcggaaaa
     1021 aatccgtcct acattgtcaa agagataccg gatcaggcgt tcgagaagat ctgtgctgcg
     1081 aatgttgtga cgctggatta catcacgaag tactacggat gagtagagtg cacctacaac
     1141 gatgttggcg gatgtttcgt catactgggc ggtgtgaatg agaggaaacc cgccgagacg
     1201 aaggtagtgc atgaaggatt cctgccggtt ctccggacag gttccggtaa tttccctgat
     1261 gaactcaaga tattccgaga aggaaagtgt ctgaacggta atctcgatgt aacgtccggc
     1321 aagataggtg gaaagttctg aggagagaag gcgggagttg gaaccggtca gatagatgtc
     1381 agtatttcct cctgcaagca gggagttgac cagtttttcc cactcaccaa cctcctggat
     1441 ctcatcgagg agaatataat atggtgggga cccggtcatt ctttcctcaa gataggagga
     1501 aaggcggtgg cgatcggtga gaggctctgc ggcggtgtct tcaaagttga ttctgaggat
     1561 gtgatccgaa gcaactcccc ggtttctaag ttcatcttcg atgagtgaca gaagagcaga
     1621 ttttccggag cggcggatac cggtgataac ttttatcaga ggtttgttta tgtggggtct
     1681 gaccttgctg aggtagcgtt cacgcgggta tatgggtgtc attgtgagca gcagtatttt
     1741 tcttttacat agtgaaaaat atttgtgttc aagataatta ttcttttcat actgaactat
     1801 tatgggttgt ttcaacgaga tgtatctctg tactgaaatg atttccgaac acgctggaaa
     1861 tagatccgca acaacagtac cgcggagtta tgagtcctgc gtattccttt tggataagcc
     1921 ccagatttaa tatacctccc gccaaacaat aaccatcttt tccaaacgga gacaaccggg
     1981 catgggcata tattttgaag taatccacaa agacatcgca ggacgcgtcg gcaaactgaa
     2041 ggtcggcgac aaaacaatca gaactccggc actgctgccg gtcgtcaacc cgcacttaca
     2101 gatcatcccg ccctcggaga tgaagaagat gggtgttgag ggaattatca ccaacgccta
     2161 catc
//
LOCUS       DALUAJ010000182         3660 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_35571,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000182 DALUAJ010000000
VERSION     DALUAJ010000182.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35571"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            37..615
                     /locus_tag="O0X37_03860"
     CDS             37..615
                     /locus_tag="O0X37_03860"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HJK62090.1"
                     /translation="MEAVIEKAVRVLARDGLVVYPTETIYGLGADALSEVAVYKVYEA
                     KQRPMGKPISVAVSDIEMIYGVAYVDGFAERFIQKFLPGPVTVVLPVKSCLPPDLSGG
                     TGTIGIRYPDHAVAQAIIAGLDSPITSTSANISGEVSPVTSDQVNVPHDYLIDGGKLP
                     GTPSTVINLATKKIERPGAMLEEIASFLKEEL"
     gene            670..1164
                     /locus_tag="O0X37_03865"
     CDS             670..1164
                     /locus_tag="O0X37_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HJK62091.1"
                     /translation="MTCTEYQKPSLPILIGSVVLCFIAAALGSLVTITGTDSWFMTEL
                     IKPECQPPSYLFGPVWTVLYLLMGIALALILAQGAERREVRVAAGMFGVQLVLNVLWS
                     YLFFGWQMIGAAAVEIILLWIVIGVTMYLFYRIRPAAAYLLIPYIAWVTFAAILTVTI
                     WTLN"
     gene            complement(1174..1641)
                     /locus_tag="O0X37_03870"
     CDS             complement(1174..1641)
                     /locus_tag="O0X37_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HJK62092.1"
                     /translation="MSLNVNDIIPNFCLQDASGREVCMPDPFAKLVVLYFYPKDNTSG
                     CTLEAKEFSELLPEFTKLGVSVYGISQDSVKSHEKFVQKQELTVPLLSDPDHVAIEGF
                     GVWVQKKLYGREYMGVDRSTFIIDGSGKILAVWNKVKAKGHAAAVLQKVQELR"
     gene            complement(1733..2467)
                     /locus_tag="O0X37_03875"
     CDS             complement(1733..2467)
                     /locus_tag="O0X37_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate
                     carboxylmethyltransferase"
                     /protein_id="HJK62093.1"
                     /translation="MIGPNRVIVRGHGREYYVRAGEGKLSTDLGMIDLAAVAELESGD
                     TIQTHLGQSFTVLVPRPTDFFSHGKRTGAPMMPKDIGMVIAYTGMSRRDRVLDAGTGS
                     GIAAIFFGGVADSVVTCEAREDFSKTAAGNIRDAGLSNVECRACDVLDVSDGPFDVVH
                     LDMMIGPKHVEHAYGLLRCGGYFATYTPFLEQTFCVMDTARRLFGDESVMTFECMERE
                     LTRSARGTRPSTKVSHTGYLTIARKF"
     gene            complement(2464..2805)
                     /locus_tag="O0X37_03880"
     CDS             complement(2464..2805)
                     /locus_tag="O0X37_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nascent polypeptide-associated complex protein"
                     /protein_id="HJK62094.1"
                     /translation="MMPGINPKQMKAAMKKMGMKMEEIEDVQKVVVYTSSGNYVFEPA
                     EVVGITMQGQTSYQLSGTPRFEPAKVEIPESDVELVASQTSVSPEAARAALEECNGDI
                     AEAILKLTAVE"
     gene            complement(2863..3357)
                     /locus_tag="O0X37_03885"
     CDS             complement(2863..3357)
                     /locus_tag="O0X37_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HJK62095.1"
                     /translation="MISSEELTSLLPVSLQRVRRIADFQFGRGAGDALFPDETTFSYS
                     NTKRIRYANLGKTRLVTVRAGDGRLTLGYAAAERLHAFLLAPKNRVVVMADAVPFILD
                     GKNAMAKHVIASDPDIMAEDEVFVVDENDNLLATGMAILSGTEMLGFSYGTAVKVRQG
                     KNRQ"
     gene            complement(3290..>3660)
                     /locus_tag="O0X37_03890"
     CDS             complement(3290..>3660)
                     /locus_tag="O0X37_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HJK62096.1"
                     /translation="GVADAGSIQWIHTHDVYPDTIVGIGAGMALSKLDPAKPIMVLCN
                     VADEPDLIKISMRTYEMVLQRGVDLQAALVAAASEFNGAGGGHNIAAGAYIPRGCEDD
                     FIRRVNELIAGQLAAGAPHC"
BASE COUNT          918 a          993 c          848 g          901 t
ORIGIN      
        1 ccctacagct atgaacgtgc agaggtaacg cctgcactgg aagcagtgat tgagaaagca
       61 gtacgcgttc ttgcacgtga cggactggtg gtgtatccta ccgagaccat ttatggactt
      121 ggggcagatg cactgtccga ggttgctgtc tataaggtgt atgaagccaa acaacgcccc
      181 atgggaaaac cgatctcagt tgcagtctca gatattgaga tgatctacgg cgttgcttac
      241 gtggacgggt tcgccgagcg gtttatccag aaatttttgc cgggaccggt aacggtagta
      301 ttaccggtga aaagctgcct cccgcccgat ctctccggtg gaaccggcac gattggaatt
      361 cggtatccgg atcatgcggt tgcacaggca atcattgcag ggcttgacag tccaatcacc
      421 tcaacatctg caaacatctc cggtgaagta tcaccagtga cctctgacca ggtaaatgtc
      481 ccgcatgatt atctgattga cggaggtaaa cttcccggaa ccccaagcac agtgattaat
      541 cttgccacaa aaaagattga acgccccgga gcaatgcttg aggagatcgc ctctttcctg
      601 aaagaggaac tctgatcttt tttggtatca tcagcccgta tttatctgtt gggcacaaag
      661 atactgcata tgacttgcac agaataccaa aaaccgtctc tcccgatact gatcggatca
      721 gttgttctct gttttattgc cgctgcactt gggtcgctgg taacaattac aggaaccgac
      781 tcctggttca tgacagaact catcaaacct gagtgccaac caccaagtta cctgttcggc
      841 cccgtctgga cggttttgta tcttctgatg ggaattgcac ttgctcttat tctggcacaa
      901 ggcgcggaac gcagagaggt gcgggttgcg gccgggatgt ttggagtgca gcttgttcta
      961 aatgtactct ggtcgtatct gttcttcggc tggcagatga tcggtgccgc agcagttgag
     1021 attatcctgc tctggattgt aataggcgta acaatgtatc tcttctacag aattcggccg
     1081 gccgctgcat acctgctgat tccctatatt gcatgggtga cgtttgcagc gatactcacc
     1141 gtgacaattt ggacgttaaa ctgaactgag gagtcacctc agttcctgta ctttttgaag
     1201 aacagcagct gcatgacctt tagccttgac tttgttccag acagcgagaa tttttccaga
     1261 accgtcaatg atgaaggtac tgcgatcaac acccatgtac tcgcgcccat agagcttttt
     1321 ctgaacccac acgccaaatc cctcaatcgc cacatgatcg ggatcggaaa gcagcgggac
     1381 ggtcagttct tgcttttgga caaatttttc gtggcttttc acgctgtcct ggctgatacc
     1441 gtaaacggaa acaccgagct tggtaaactc aggcagaagc tcggagaact cttttgcctc
     1501 cagcgtacat cccgaagtat tgtcttttgg ataaaagtag agcactacaa gcttcgcgaa
     1561 tgggtccggc atacagactt cgcggccgga ggcgtcctgc aggcaaaagt tagggatgat
     1621 gtcatttaca ttcagtgaca tgctttcaac gtatgcagga gacagacata aagtatcagg
     1681 ttgtggaaag tcagtacaaa taccgcacaa cgttagcaga caaaaaaagt attcagaatt
     1741 tccgtgctat tgtcagatag ccggtatgag atacctttgt cgatggccgg gtaccccttg
     1801 cactccgcgt cagttcacgt tccatacact caaaggtcat tacagattcg tcaccaaaca
     1861 accgccgcgc cgtatccatc acacaaaatg tctgctccaa aaacggagtg tacgttgcaa
     1921 agtacccgcc gcagcgaagc agaccataag catgctccac atgctttggc ccgatcatca
     1981 tgtcgaggtg cactacatca aacgggccgt cagagacatc cagtacatca cacgcccggc
     2041 actcaacatt ggacagcccg gcatcacgaa tatttccggc agccgttttg gaaaaatctt
     2101 cacgtgcctc gcaggtcacc acactgtctg caacgccacc aaagaagatt gccgcaatcc
     2161 cagaaccggt acccgcatca agtacgcgat cacggcgaga catccccgta taggcaataa
     2221 ccataccaat atcctttggc atcatcgggg caccggtacg ttttccatga gagaagaagt
     2281 ccgtcggacg cggtaccaga acagtaaatg actgccctag atgggtctga atagtgtcgc
     2341 cgctttcaag ctcagcaacc gccgcaagat cgatcatccc gagatctgtt gagagttttc
     2401 cctctcccgc acgcacatag tactcgcgcc cgtgcccccg gacaatcact ctgtttggtc
     2461 cgatcattct actgccgtca gtttcagaat tgcctcggca atgtctccgt tacactcctc
     2521 aagagcagca cgggcagctt ccggggacac agaagtctgg gaggcgacaa gttccacatc
     2581 tgactcgggg atctccactt ttgcaggttc aaaccggggg gtaccggaca gctgatagga
     2641 ggtctgcccc tgcattgtaa taccaacaac ctcggcaggc tcgaagacgt aatttccgga
     2701 ggaagtataa acaacgactt tctggacatc ttcaatctcc tccatcttca tgcccatctt
     2761 cttcattgca gccttcatct gtttcggatt aatacctggc atcatagtta tcaaccaatt
     2821 ctcgttactg tttttattca aagattctta attccatcgg gattactgcc ggtttttgcc
     2881 ctgacgtacc ttcactgctg ttccgtagga gaagccaagc atctcggttc cagaaaggat
     2941 cgccatacca gttgcaagca gattatcatt ttcatccaca acgaacactt catcctctgc
     3001 cataatatcc ggatcacttg caatcacatg ttttgccatc gcattctttc cgtcaaggat
     3061 aaacggcaca gcatccgcca tcaccacaac acggttcttt ggggcaagga gaaatgcatg
     3121 aagacgctcc gctgcagcat aaccaagggt tagtcgacca tctcccgcac gcacggtgac
     3181 aagtctggtt ttaccaagat tcgcatatcg gatacgcttt gtatttgagt aggaaaacgt
     3241 tgtttcatcg ggaaacagcg catctcccgc ccccctgccg aactggaaat cagcaatgcg
     3301 gcgcacccgc tgcaagctga ccggcaataa gctcgttaac tcttctgatg aaatcatctt
     3361 cacaacctct ggggatatat gcccccgctg caatattatg tcctccgcct gcaccattga
     3421 actcagatgc tgcagcaacc agcgccgcct gtaaatcaac accgcgctgg agtaccatct
     3481 catacgtacg catcgagatt ttgatcagat ccggctcgtc tgcaacatta cagagcacca
     3541 tgattggttt tgccgggtcc agtttggaca gggccattcc tgcaccaatc ccaacgatcg
     3601 tatccggata tacatcgtgt gtgtgaatcc attgtatact gcctgcgtct gcaacaccgg
//
LOCUS       DALUAJ010000183         2753 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_35597,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000183 DALUAJ010000000
VERSION     DALUAJ010000183.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35597"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="O0X37_03895"
     CDS             <1..538
                     /locus_tag="O0X37_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62097.1"
                     /translation="SCTGQVLVSLPDSAADLTILLAGARQTGSVPAFWYVSGDVILGD
                     PALVQSTLIYDSLIGSDIWSSELGKSKPLVREVSLFYSSVYPGKSMTGVSARSFEAVV
                     MLADAMSVSGSTAPSSVGSALKTLNYSADQSIFFGDGVSFDASGVAIGPETMLLQVQS
                     GTTRIISSSADLRLMTKD"
     gene            638..>2753
                     /locus_tag="O0X37_03900"
     CDS             638..>2753
                     /locus_tag="O0X37_03900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HJK62098.1"
                     /translation="MTIRYLRFLVGGMHCSACSAHLESTLKALPGILEVTVNLSTATV
                     SANIDTDLVTPEKIDEAVRSAGFSVNSVPVSFIVTPADDVSISAAAKRALQELSGVQD
                     VSESGDLVTILYHPLLADPSQFVSVLKKAGYSSVTVNSGFDDLFYIPEHEKDELSGLR
                     NRTILGFIGSAVLMGLMLFVHPLFAEDMFLMDILMLVIATPILVVCAYPIFQQGISQL
                     RGGMLGMEVMYMLGITTSYIAGLGAVAGYLPSPDFLLFETAVMLTAFLGLGRFLETRA
                     RGRTSDAILALLHLRPPVATVIRDGVAQEVRVGELVAGDTVLVKTGGQIPSDGIVLEN
                     PVTVNESAITGESVPVVKMPGTSVIGGTVVSQGVLTMEVEKVGADTVLAGIIKMVREA
                     QETKPPVQRLADAAVRYFIPFVLSVAIIAFVYWFFFAGETLLIALSSFIAVIVVACPC
                     ALGLATPTAVTVGIGRAASLGILIRNGEVLERAGKLKAVAVDKTGTLTVGSPTVSEVV
                     PAPGTSEEDLLAIAASLGRKSLHPLDSAIVAEADKRGIADLAAARVETVPGRGMTARV
                     KRETVVIGSAEFMEEMSIIVDDLVSSAAEKFRAAGSSVVFVAVAGKVVGAVGISDALR
                     PDAVRAVAEFESMGLPVTMLTGDNARAAEVVAGLAGISHVEAGLMPDEKRTIIQHMQE
                     EGKAVVFVGDGINDTPAMTQADV"
BASE COUNT          581 a          573 c          800 g          799 t
ORIGIN      
        1 atcctgtacc gggcaggttc ttgtaagttt acctgatagt gcagcggatt tgaccattct
       61 tcttgctggt gcccggcaga ctgggtcagt ccctgctttc tggtatgttt caggggatgt
      121 gattcttggt gatcctgcct tggtacaaag tacactgatt tatgattctc ttattggttc
      181 agatatctgg tcctcagaac ttggaaagtc aaaaccgctg gttcgagagg tgtcactgtt
      241 ctattcaagt gtgtatcctg gaaagtcgat gactggtgta tctgccagat catttgaggc
      301 ggtggtgatg cttgccgatg ctatgagtgt gtccggatcc actgctccat cgtcagttgg
      361 ttctgctttg aaaaccctca actactctgc agaccagagt attttctttg gtgacggtgt
      421 ctcatttgac gcttctggtg ttgctattgg tccggagacg atgctcttac aggtccagag
      481 tggtactacc cgtatcatca gcagttctgc agatcttcgt ctgatgacaa aagactaacc
      541 tgattttttc tttttggtgc tgtctacacg ttattcctgt tatttttcgt gaaccctaac
      601 agagtctatt tgtgtagtga ggaataacat atggttcatg acgattcgtt acctccgatt
      661 tctggttggc ggcatgcatt gcagcgcatg ctcggctcat cttgaatcca cactaaaggc
      721 cctccccggt attttggagg tcacggtcaa tctttccaca gcaactgtga gcgcaaatat
      781 cgatacggat ctggtaaccc cggaaaagat agatgaagca gtccgatcgg ctgggttctc
      841 ggtgaactca gtgccggtct cgtttattgt aactcctgcg gatgatgttt ccatatcagc
      901 tgctgcaaag cgtgcactac aggaactttc tggcgtgcag gatgtttctg agagcgggga
      961 tctggtaacg atcttgtatc atccgttgct tgcagaccca tcacagtttg tttcagtttt
     1021 gaaaaaggca ggttattctt cggttaccgt gaacagcggg tttgatgatc tgttctatat
     1081 ccctgagcat gagaaggacg aactgtctgg tttgcgaaac agaacgattc tcggatttat
     1141 cggttcagca gttttaatgg ggttgatgct gtttgttcat cctctgtttg cggaagatat
     1201 gtttctgatg gatattttga tgctggttat tgcaacaccg attctggttg tatgtgcgta
     1261 tccgatcttc caacagggga tctcccagct tcgcggaggg atgctcggta tggaggttat
     1321 gtatatgctg ggtattacga cgtcgtatat tgcgggtctt ggtgctgttg caggatattt
     1381 accatcacca gactttctgc tgtttgaaac tgctgtgatg cttaccgcat ttttaggact
     1441 tggccgattt ctcgaaacac gggcgcgggg gcgaacatcg gatgcgattc ttgcactgct
     1501 gcatctccgt cctccggttg caacggtcat tcgtgatggt gtagcccagg aggtgcgagt
     1561 tggggaactt gtcgcagggg acacagttct ggtaaagaca ggtggtcaga ttccatcgga
     1621 tggtatagtt cttgaaaacc cggttacggt gaatgagtct gcaattactg gtgagtctgt
     1681 gccggtggta aaaatgccgg gaacgagcgt tattggtggt actgtggtgt cacagggagt
     1741 tctcacgatg gaggtggaaa aggtgggggc agacactgta cttgcaggga tcattaaaat
     1801 ggtgcgggaa gcacaggaga ctaaacctcc tgtacagcgg cttgcagacg cagccgtccg
     1861 gtactttatt ccgtttgttc taagtgtagc aattattgca ttcgtgtact ggttcttctt
     1921 tgccggcgag acactgttga ttgcattgtc gagtttcatt gcagtaattg tggttgcctg
     1981 tccctgtgca ctgggacttg caacaccgac tgcggtgact gtaggtattg gtcgggcggc
     2041 aagtcttgga attttgatcc ggaatggtga ggtactggag cgtgcaggga aactgaaagc
     2101 cgttgcggtg gataagacgg gtacactaac agtaggttct ccaacagtat ctgaagtagt
     2161 acctgcccca ggtacaagcg aagaagatct ccttgcgatc gccgcatctc ttgggcggaa
     2221 atctttgcat ccacttgact cggcgattgt tgcagaggcg gataaacgcg gtattgcgga
     2281 tcttgcggcg gcacgggtgg aaactgttcc cggcagggga atgacggcac gggtgaaacg
     2341 tgaaactgtg gtgataggat cggccgagtt catggaggaa atgagtatca tcgtggatga
     2401 tttagtatca tctgctgcgg aaaaattccg tgcagcggga tcgtcggtgg tgttcgttgc
     2461 tgtcgccgga aaggttgtgg gagctgttgg gatctccgat gcactccgac ctgacgccgt
     2521 ccgtgccgtt gcggagtttg agagtatggg ccttccggta acgatgctga caggggataa
     2581 tgcacgtgct gctgaggttg ttgctggcct tgctggcatc tctcatgttg aagcaggtct
     2641 gatgccggat gagaaacgta cgattatcca gcacatgcaa gaagagggaa aggcagtggt
     2701 gttcgtgggt gatggaatta atgatactcc ggcgatgacg caggctgatg tag
//
LOCUS       DALUAJ010000184         3097 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_35756,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000184 DALUAJ010000000
VERSION     DALUAJ010000184.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35756"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="O0X37_03905"
     CDS             <1..496
                     /locus_tag="O0X37_03905"
                     /inference="COORDINATES: protein motif:HMM:NF023212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3320 domain-containing protein"
                     /protein_id="HJK62099.1"
                     /translation="LDSYHQFASVPDSVLGTAIVQIVAIEGPISPSVLAARIKELGRV
                     PRMTAAVRNRIASAAETEVSEGRLSADEEGFLTVPECVISPRERPAKWSAADISLAEI
                     STAAVSVLEKQFSTPRDDLIRQTAVVLGFKATAAVKERIASGIDAGIADGRILLEGNI
                     CKAA"
     gene            565..1251
                     /locus_tag="O0X37_03910"
     CDS             565..1251
                     /locus_tag="O0X37_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HJK62100.1"
                     /translation="MSEAVSAVLTSAPYLLEGMCVTLALVALALLIGFLCGIVLTIGQ
                     VYGPRYIKRLVDIYVWFFRGLPNIVLLFLFYFALFPMTGFDVPAFAVAVVVLGLRSAA
                     YQSQIFRGAIESIADGQMLAARSLGMTKWQSIRTVILPQALRLSLPGWSNEYPVLLTD
                     SAVAYVIGVAELLTRTAQVISKTGEPMVLYLTCAVIFIILNYGGMMLIQRVEKKVRIP
                     GFGNNEVTET"
     gene            1248..1988
                     /locus_tag="O0X37_03915"
     CDS             1248..1988
                     /locus_tag="O0X37_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HJK62101.1"
                     /translation="MSDTPILRVENLSKSYGDLEVLKSVSFDVYKGETKAFIGPSGTG
                     KSTLLRCINQLTVPDDGHVYLNGEEITHSGSKINEYRQKMGMVFQNFNLFDHLTAVRN
                     VEIALLKVKKMTKEEARKKAMFELERVGMADRADYYPAQLSGGQAQRVSIARALAMDP
                     DVMLFDEPTSALDPELKREVLEVMRHLAADGMTMLIVTHEMHFATSFASEILFMEKGE
                     IVERGSPADIPTCPSFERTRAFMGTYEE"
     gene            1991..2746
                     /locus_tag="O0X37_03920"
     CDS             1991..2746
                     /locus_tag="O0X37_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HJK62102.1"
                     /translation="MDAATTVTTNATALSGVSEFLNELWAYIVSEIPFWQDILLPALW
                     DGLLVTLQLVVLTAPFGFLLGLLVAVARVYGVKPVRWAAQLYVIFFRGCPLLLLLFIL
                     YFGLPSIGIVLGSYLSALIGFILCNSAYNSEYIRGALQSIKGGQMTAAQALGMTKLQA
                     VRYIVIPQALRKAVPGVSNEFIYLIKYSSLAYVVTVIELTGAAKIIASKYFAYFEAFA
                     AVGVFYLVIVTIATIAINRLEKKYAIPGTGAGR"
     gene            2789..3040
                     /locus_tag="O0X37_03925"
     CDS             2789..3040
                     /locus_tag="O0X37_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5654 family protein"
                     /protein_id="HJK62103.1"
                     /translation="MSFSLDVIDKLSALITAAFGLVAALAWNSAIQEIFKEVFGEQSS
                     IPAMLGYAVFVTVIAVIFTLWIGFVSNKAKEKIVPEKKE"
BASE COUNT          633 a          792 c          803 g          869 t
ORIGIN      
        1 ccttgacagt taccatcagt tcgcctctgt acctgactcg gtccttggaa ccgccatcgt
       61 tcagatcgtc gcaattgaag gtcccatctc cccgtctgtt cttgcagctc ggatcaagga
      121 actgggccgc gtgccccgca tgaccgcagc ggttcggaac cgtattgcct ccgctgctga
      181 aacagaggtt tccgagggaa gactttccgc ggatgaggaa ggattcctga ccgttcctga
      241 gtgtgttatc tctccgcgtg aacgacctgc caagtggtct gctgcagaca tttcccttgc
      301 cgaaatatct actgcggccg tttcggtttt ggaaaaacag ttctccactc cgcgtgatga
      361 tctcatccga cagacggccg ttgttcttgg cttcaaagca accgctgctg ttaaagaacg
      421 gattgcatca ggtattgacg cgggtattgc cgatggccgt atccttcttg aaggaaatat
      481 ctgcaaagct gcctaacgaa tccatcccta cacttttttc tgatatcgca tcacagtata
      541 tactacttct ttgggggaag tcctatgtct gaggcagtat ccgcagtact tacatccgct
      601 ccctatcttc tggagggaat gtgcgtaacc cttgcactgg tagcactggc gctcctgatt
      661 gggttccttt gcggcattgt tctcaccatt ggacaggtgt atggtccccg ctacattaag
      721 cgtctggttg acatctacgt ctggttcttc cgcggcctgc cgaatattgt acttctgttt
      781 ctgttctact ttgcgctctt cccgatgacc ggttttgatg tgccggcatt tgcagttgcg
      841 gttgtagtac ttggtctcag aagtgccgca tatcagtccc agatcttccg cggggccatt
      901 gagtcaattg ccgacggtca gatgcttgcc gcacggtccc ttggcatgac aaagtggcag
      961 agtatcagaa cggtgatcct tccccaggcc ctccggctct ccctgccggg atggtccaat
     1021 gaatacccgg tccttctgac ggactccgcc gttgcctatg tgattggtgt tgcagagctt
     1081 cttaccagaa ccgcgcaggt catcagcaaa accggtgagc cgatggttct gtatcttacc
     1141 tgtgcggtta tcttcatcat tcttaactac ggcggcatga tgctcatcca gcgggtggaa
     1201 aagaaagtcc ggatccccgg attcggcaac aatgaggtga cagaaacatg agtgatactc
     1261 caattctccg tgttgaaaac ctctccaaat cctatggtga tctggaggtt ctcaagtcgg
     1321 tatcgtttga tgtctataaa ggcgaaacca aggcgtttat cggcccctcg ggtacgggaa
     1381 agagtacgct tctgcgctgt atcaatcagc tgaccgttcc ggacgatggt catgtgtacc
     1441 tcaacggtga ggagatcacg cactccggat ccaaaattaa tgagtaccgc cagaagatgg
     1501 ggatggtgtt tcagaacttt aacctgtttg atcatctgac cgcggtccgt aacgtggaga
     1561 ttgcccttct caaggtcaaa aagatgacca aggaagaggc ccgcaagaag gccatgttcg
     1621 agcttgagcg ggttggtatg gcggaccgtg ccgactacta tccggctcag ctttccggcg
     1681 gtcaggctca gcgtgtgtcc attgcccggg cacttgcaat ggatcctgat gtcatgctgt
     1741 ttgatgagcc gacctcggct cttgatccgg aactgaaacg cgaagtgctg gaggttatgc
     1801 ggcacctcgc cgcagacggt atgacaatgc tgattgtcac gcatgagatg cactttgcca
     1861 cctcgtttgc cagtgagatt ctgtttatgg agaagggaga gattgttgag cgcggcagtc
     1921 ctgctgatat tccgacctgt ccgtcgtttg aacgcacccg tgcctttatg ggaacatacg
     1981 aagagtagtc atggacgcag cgacaaccgt taccaccaat gcgacagcgc tgtccggtgt
     2041 atccgaattc ctgaatgagc tttgggctta catagtttcg gagattccgt tctggcagga
     2101 cattctcctt cccgcgctgt gggatggtct gcttgtcacg cttcagctgg ttgtgcttac
     2161 tgcaccgttc ggtttccttc ttggcctcct ggttgcggtt gcccgcgtgt acggcgtgaa
     2221 accggttcgc tgggcagcgc agctgtatgt gatcttcttc cgcggctgtc cgcttctgct
     2281 tttgctgttt attctgtact tcggtctgcc atccattgga atcgttctgg gttcctatct
     2341 ctcggcactg ataggattta ttctctgtaa ctccgcctac aactccgagt acatccgcgg
     2401 tgcacttcag tccataaaag gcggtcagat gaccgcagcc caggctcttg gtatgacaaa
     2461 gcttcaggcg gtccgctaca ttgtcatccc acaggcactt cgcaaagcag ttcccggtgt
     2521 ttcaaacgag tttatctatc tgatcaaata ctcctccctt gcctatgtgg tgacggtgat
     2581 tgaactgacc ggtgcggcca agattattgc aagtaagtac tttgcctact ttgaggcgtt
     2641 tgcagcagtt ggagtgtttt atctggtgat tgtgaccatt gcaactattg cgatcaaccg
     2701 tcttgaaaag aagtacgcaa tccccggtac cggtgccggg agatgaggtg ttacggttta
     2761 agtatttttg atccctaata gacactgcat gtcatttagt cttgatgtga tcgataagct
     2821 gtctgcactt attaccgccg catttggtct tgttgccgca cttgcatgga actccgccat
     2881 tcaggagatt ttcaaggagg tctttggtga gcagagcagt attcctgcga tgcttgggta
     2941 tgcggttttt gtaacggtca tcgctgttat cttcactctt tggattgggt tcgtttccaa
     3001 caaggcaaag gaaaagatcg tgcccgagaa aaaggagtga tccttttctc cacttttttg
     3061 aacaatattt attatcgggt attttcatac gtgtata
//
LOCUS       DALUAJ010000185         1733 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_35897,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000185 DALUAJ010000000
VERSION     DALUAJ010000185.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35897"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="O0X37_03930"
     CDS             <1..307
                     /locus_tag="O0X37_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine permease"
                     /protein_id="HJK62104.1"
                     /translation="ITIPTVITGCLLIFIMTSQLSAALGVLIEHEGTGVFSFNNGVVI
                     GFSILIAATFSFLPDTAVAAIPAVIRPVLANGFVAGTVAVMLLEHVVFREKKTETEQ"
     gene            complement(336..524)
                     /locus_tag="O0X37_03935"
     CDS             complement(336..524)
                     /locus_tag="O0X37_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HJK62105.1"
                     /translation="MVEMMTTETAYAILGVPVGSDFATTAKAFRELRDKYNPETNPFR
                     RKEFAEVMAAFEFLQRQF"
     gene            complement(570..1268)
                     /locus_tag="O0X37_03940"
     CDS             complement(570..1268)
                     /locus_tag="O0X37_03940"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HJK62106.1"
                     /translation="MCEDTNCLCGAFEDECGCEGFYHEMPRLGEPAPDFVAETTHGQI
                     KLSDYKDKKWVVLFSHPADFTPVCTTEFVAFSKEYSEFEKRNVALIGLSVDSSSSHLA
                     WVRDIEANFGVHVPFPIIADLDMGVASLYGMIQPAMSSTAACRAVFFIDPKGILRAMI
                     YYPLTTGRCVAEILRVIDALQVNDEHHVATPANWKPGDKVIVPAPKTQKEMDEREKIT
                     GPDKKTWYLLMKDL"
     gene            1490..>1733
                     /locus_tag="O0X37_03945"
     CDS             1490..>1733
                     /locus_tag="O0X37_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834080.1"
                     /note="involved in the sodium dependent uptake of proline;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proline symporter"
                     /protein_id="HJK62107.1"
                     /translation="MLDWNLIGIGAAFIIYFAAMIYIGFFYFKKTKTTSDYVLGGRKL
                     HPFVAAMSAEASDMSGWLLLGLPGLAYATGMSAAGWT"
BASE COUNT          461 a          452 c          411 g          409 t
ORIGIN      
        1 catcaccatc ccgaccgtaa tcacgggatg ccttctcatc ttcatcatga ccagccagct
       61 ttccgcagca cttggtgtcc tcatcgaaca tgaaggaaca ggagtgttct ccttcaacaa
      121 cggagttgtg atcggcttct caattctgat cgcggcaaca ttttcattcc tgccggacac
      181 agcagttgcc gcaatcccgg cagtcatccg gcccgtactt gcaaacggat ttgtcgcagg
      241 aactgtggca gtaatgctgc tggaacatgt agtgtttcgg gaaaagaaaa cagagacaga
      301 acaataacat ccaaaaaaac aaaaaattgc gaggatcaga actgccgctg caaaaactca
      361 aaggcagcca tcacttcggc aaactctttt ctgcggaagg gattcgtctc cggattatat
      421 ttatcacgaa gctcacggaa tgcctttgca gtcgttgcaa aatccgaccc aaccggaaca
      481 ccgagaatcg catacgccgt ctcggtcgtc atcatctcaa ccatactact tacctcaggc
      541 ggcaaaagag aaaaagtttg aggaaggatc tacagatcct tcatcagaag ataccaggtc
      601 ttcttatccg gacccgtgat cttctcacgc tcatccatct ctttctgtgt ctttggcgcc
      661 ggaacaataa ccttgtcccc cggtttccag tttgcaggcg ttgcaacatg atgctcatca
      721 ttcacctgca aagcatcaat cacccggaga atctctgcca cacagcggcc ggttgtcagc
      781 ggatagtaga tcattgcacg gagaattcct ttcggatcaa tgaagaacac cgcacggcag
      841 gcagcagtcg aactcatcgc cggctgaatc ataccgtaaa gggaagcaac tcccatatcc
      901 agatccgcaa taatcgggaa cggtacatgc acgccaaagt tcgcctcaat gtcccttacc
      961 catgcaaggt gggaagagga actgtcaacc gacagaccga ttaacgcaac atttctcttc
     1021 tcaaactcgg agtactcctt tgagaacgcg acgaactccg tcgtacagac cggggtaaag
     1081 tctgccggat gggagaacag cacgacccac ttcttatcct tatagtctga gagtttgatc
     1141 tggccgtgag tagtctctgc aacaaaatca ggagccggtt ctccgagacg gggcatctca
     1201 tggtagaaac cttcgcagcc gcactcatcc tcgaatgcgc cgcaaagaca gttggtatct
     1261 tcacacattt ttgtggttca ccttgtatgt ctgttgaatc catgaaggaa taagtgttca
     1321 gttaacggca aaatgacgga ttcggatcgg aaaaaccgtt tcgaagtctg tgtaagagca
     1381 cataaatagc atgttaacga attaagtaag ttgcagtaac tttgtgtatt tgtacccggc
     1441 agcccggtgt ggggcaggcg ggagaaatac cactgccggg ggaatatttg tgttagactg
     1501 gaacttaatt ggcattggag cggcgttcat catctacttc gctgcaatga tctatatcgg
     1561 gttcttctac ttcaagaaga ccaaaaccac aagtgactac gtgcttggag gacgtaaact
     1621 gcatccgttt gttgcagcaa tgtccgccga agcatccgat atgagcggat ggctgctcct
     1681 tggtctgccg ggtcttgcgt acgcaaccgg tatgtcggca gccggctgga ccg
//
LOCUS       DALUAJ010000186         3692 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_36108,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000186 DALUAJ010000000
VERSION     DALUAJ010000186.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36108"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(66..572)
                     /locus_tag="O0X37_03950"
     CDS             complement(66..572)
                     /locus_tag="O0X37_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJK62108.1"
                     /translation="MPKTLRIPFVRDDPILIRGCSSFLLVARSREKFPLIIETDDEEL
                     VQGVYPGDLIVVSAPEGGEIPPALYLLEMVNTYHTPVIALGKDHPASRRLSYVISAAE
                     RIEMRCDIRRGTHPEQHLLCAADEFTGMILYAEEEDLVIENCREELRLSYMTWNLSLA
                     ETTYQNCG"
     gene            complement(575..733)
                     /locus_tag="O0X37_03955"
     CDS             complement(575..733)
                     /locus_tag="O0X37_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62109.1"
                     /translation="MCSAGGPVDVDFNDPLKGKGKKYRCNECGQTFTGLGKHPMCPSC
                     QSEDVTEI"
     gene            complement(765..1283)
                     /gene="hpt"
                     /locus_tag="O0X37_03960"
     CDS             complement(765..1283)
                     /gene="hpt"
                     /locus_tag="O0X37_03960"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HJK62110.1"
                     /translation="MADTIEVLIQEEQIDARIRELAKQIDADYAGKSVVLLTTLKGAC
                     FFACELAKRLTIPVFMEYIQAASYEGKSSTGTLTMKLDIPADAITGKHVIIIEDVIDT
                     GRTLSAVKELIRSRSPASLAVCSLLDKHECRVVPFEGDYIGFSIGNDFVVGYGLDWDQ
                     KYRNLPYIGIVR"
     gene            complement(1350..>3692)
                     /gene="carB"
                     /locus_tag="O0X37_03965"
     CDS             complement(1350..>3692)
                     /gene="carB"
                     /locus_tag="O0X37_03965"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HJK62111.1"
                     /translation="VQGGCNIQFAFRNGDYRVIEVNPRVSRSSALASKATGYPIARVA
                     AKIAIGMHLDEITNTVTGTTPACFEPAVDYVVVKVARWPFDKFKTADRTLTTAMKSTG
                     EVMAIGRTVEEGFKKALRSLDTDIIHHTDLNEIKMILSRPTDERFPTLFDAFRLGMTV
                     EDVYKLTQIEPFFLEKIQHVVDIELELREHPTEELVKTAKKFGFSNAEIRELTGWNIY
                     KIEQLVGLPTYKMVDTCAAEFPAKTPYYYSTWEQECELKDTPNKKVLILGSGAIRIGQ
                     GIEFDYCTVHAVKSLREEGIEVHILNNNPETVSTDFDTSDRLYFEPMQLEDVVNILRK
                     GDYDGVMVQFGGQNSVNLAIPIQEEIKLFGLKTKILGTSPDNMDVAEDRNRFSVLLEK
                     DNIPSPANGSAYSEKEAYEIAKKIGYPVLVRPSYVLGGRAMELVHDELELQTYIKEAV
                     RVSNTHPVLIDRYLDNATELDVDAVSDGTDVLIGGIMEHIEEAGVHSGDSACVIPTQT
                     LTAEQIATVKDYTRKIALSLGVVGLINIQYALHNGTIYVLEANPRASRTVPFVAKATG
                     IPLAKIAAKVMLGKKLADLGYHEKEIKHVAVKEVLLPFSRLPGVDPILGPEMKSTGEV
                     IGIDYDFGRAFYKASQAADNKLPLEGNVFISITDDQKTAILPIAQKLTDLGFNLYGTE
                     GTVKFLEQHNIKMNLVRKVQEGSPNIIDMVRGADIDLVINTPGDKNARADHFQIMRAT
                     IDYSIPYITTIFGAEAAVQAIESMKSNKITIEPLSHYHSE"
BASE COUNT          828 a          917 c          951 g          996 t
ORIGIN      
        1 gtaggaattg tttgatcacc attctccaac aaaaaatgtg tcagggatgt gagacagagg
       61 agtgttcagc cgcagttctg gtatgttgtt tctgcgaggg agaggttcca ggtcatatat
      121 gacagtctga gttcttcacg acagttttcg atcaccagat cctcttcttc ggcatacaga
      181 atcattccgg tgaactcatc tgcggcgcag agcagatgct gttctggatg cgtaccgcgt
      241 ctgatgtcac accgcatctc gatccgttct gcagctgaga tgacatatga gagtctccgg
      301 cttgcgggat gatcttttcc taaggcgatc accggcgtgt gataggtatt aaccatctcc
      361 aaaagataca gagcaggtgg tatttctccg ccttcgggag cagagaccac aatcagatct
      421 cccgggtaca ctccctgcac aagttcttca tcatcggttt cgatgatcaa cggaaacttt
      481 tcccgggaac gtgcaacgag caaaaaagaa gagcatcctc tgatgaggat tggatcgtcc
      541 cgcacaaacg ggatacggag agtttttggc attgttatat ctcagtgacg tcctcggact
      601 ggcaggaggg gcacatcgga tgcttgccaa ggccggtaaa tgtctggccg cactcgttgc
      661 accggtattt tttgcctttt cctttcagag gatcgttgaa atcaacatcg actggtccgc
      721 ctgcggaaca cataccagaa catcgacgcc tgagatgata agcgttaccg cacaatcccg
      781 atatagggaa ggttgcggta cttctgatcc cagtcaagac cataaccgac aacgaaatcg
      841 ttgccgatac tgaatccgat gtaatctcct tcaaacggaa caacccgaca ctcatgcttg
      901 tccaaaagcg aacataccgc aagtgacgcc ggactgcggc tgcgaatcag ctctttaaca
      961 gcggagagtg ttctgccggt gtcaataaca tcctcaataa tgatcacatg ttttccggtg
     1021 atcgcatctg ctgggatgtc cagcttcatg gtgagggttc cagttgaaga ttttccttcg
     1081 tagcttgctg cctgaatgta ctccataaag acggggattg tcaggcgttt ggcaagttcg
     1141 catgcaaaaa aacaggctcc tttcaaggtg gtaaggagga ctacggattt tccggcatag
     1201 tctgcatcga tctgttttgc aagttctctg atgcgtgcgt cgatctgctc ttcttggatc
     1261 agcacctcta ttgtatccgc cataaactct ggtatgtata gagcagagta tgaaataatt
     1321 ctgccgatcg tttgaaaaaa gaaaaaaaat tattcggagt ggtagtgcga aagcggctca
     1381 atggtaatct tgtttgattt catcgactcg atagcctgca cagctgcttc tgctccgaag
     1441 atggttgtga tgtaggggat actgtaatcg attgttgccc gcatgatctg gaagtgatct
     1501 gcccgtgcat tcttgtctcc cggcgtgttg atcaccagat cgatatctgc accccgcacc
     1561 atatcgatga tgttcgggga tccttcctgc acttttctga ccagattcat tttgatgttg
     1621 tgctgctcca ggaacttcac tgtaccttcc gttccgtaga ggttgaagcc aagatctgta
     1681 agtttctggg caatcgggag aattgcagtc ttctgatcgt cggtgatgga gatgaacaca
     1741 tttccttcca gcggaagttt attgtctgct gcctggcttg ccttgtagaa tgcgcggccg
     1801 aagtcgtagt caataccgat aacctcaccg gtcgatttca tttccggacc aagaatcgga
     1861 tcaacacccg gcagccgtga gaacggcaga agaacttctt tgactgcaac gtgcttgatc
     1921 tctttctcgt ggtatccgag atctgcaagt ttcttaccga gcatcacctt tgccgcaatc
     1981 tttgcaagcg ggataccggt cgcttttgca acgaacggaa ctgttctgct ggcacgcggg
     2041 tttgcctcaa ggacatagat ggttccgttg tgcagggcat actggatgtt gatcagaccc
     2101 acaacgccaa gggagagtgc aatctttctt gtgtagtctt ttacggttgc aatctgttct
     2161 gcggtcagtg tctgcgtcgg aatcacacag gcagagtcac cggagtggac acctgcctct
     2221 tcgatgtgtt ccatgatacc gccgatgaga acatcggttc cgtcggacac tgcatccaca
     2281 tccagttcgg tcgcattgtc gaggtagcga tcgatcagca ccgggtgtgt gttcgagaca
     2341 cggactgcct ctttgatgta ggtctgcagt tcaagttcat cgtggaccag ttccattgca
     2401 cggccaccga gcacataact tggccggacc agcaccgggt agccgatctt ctttgcgatc
     2461 tcatatgcct ctttctcgga gtatgcagat ccgtttgccg gggatgggat gttatctttc
     2521 tccagcagaa cgctgaaccg gtttctgtcc tcggccacat ccatgttgtc cggactggtt
     2581 ccaaggattt tggtcttgag accgaacagc ttgatctctt cctgaattgg gatcgcaagg
     2641 ttaacggagt tctgaccgcc gaactgtacc attaccccgt cgtagtctcc ttttctcagg
     2701 atattgacaa catcctccag ctgcatcggc tcaaagtaca accggtcgga tgtgtcgaag
     2761 tcggtggaaa cggtctctgg gttattgttg aggatatgta cctcaattcc ttcctcacgc
     2821 agggacttga ctgcatgtac agtacagtag tcgaactcga taccctgacc gatacggatt
     2881 gcaccggaac cgaggatgag cacttttttg tttggtgtgt ccttcagctc gcactcctgc
     2941 tcccaggtgg agtaatagta tggtgtcttt gccgggaact ctgctgcaca ggtatcgacc
     3001 atcttgtagg taggaaggcc cacgagctgt tcaatcttgt aaatgttcca gccggtcagc
     3061 tcccggatct ctgcgttgga gaatccgaac ttctttgcgg tcttgaccag ttcttccgtc
     3121 gggtgttcgc ggagttcaag ctcaatgtca accacgtgct gaatcttctc caggaagaac
     3181 ggttcaatct gggtcagctt gtagacatcc tccactgtca taccaaggcg gaatgcatcg
     3241 aacagcgtcg ggaaccgttc atccgtcggg cgggagagga tcatttttat ttcatttaag
     3301 tcggtgtggt ggatgatgtc ggtatcaaga gaacgaagtg ctttcttgaa tccctcttcg
     3361 acggttctgc cgattgccat tacctcaccg gtagacttca ttgcagtggt gagcgttctg
     3421 tctgcggtct tgaacttgtc aaacggccag cgtgcaactt tgaccaccac gtagtcaaca
     3481 gccggctcaa agcatgctgg cgttgtaccg gtgacggtgt ttgtgatctc gtcaaggtgc
     3541 ataccgattg cgatctttgc ggcaacacgg gcgatcgggt aaccggttgc tttggatgca
     3601 agggcagagg accgagatac acgcgggttg acttcaatga cccggtaatc tccgtttctg
     3661 aaggcaaact gaatgttaca tcctccctgc ac
//
LOCUS       DALUAJ010000187         1948 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_36140,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000187 DALUAJ010000000
VERSION     DALUAJ010000187.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36140"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="O0X37_03970"
     CDS             <1..960
                     /locus_tag="O0X37_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62112.1"
                     /translation="VRDGLSIDDIVNIRSRTLRGGKELDVKVPDVGKVQEIALSEKPL
                     DIEVAFTKPVQFHRNFDGTVEPVGMSGTMKKLDVIDNARVSRVVDRCTSDTDLRATEA
                     ARILFENGTDVYKITNLLTAGLLGVQRKVVPTRWAITATDDMISSGMKKDVIRMPALP
                     EYQVFCATLHGNTLCFLLIPSEEWRFEMIERWQKHSLWAGDEETVIEDGETGLSKSKY
                     SPIGGAYYSARLAVLEYLKSIGRCAKVLCVRDISGEYWAPLGTWVIREASHVSLRTAP
                     IRVGTLGEATTAAAAGLRTNFWIPYAKMLKDIKQQKTLADFFS"
     gene            957..1598
                     /locus_tag="O0X37_03975"
     CDS             957..1598
                     /locus_tag="O0X37_03975"
                     /inference="COORDINATES: protein motif:HMM:NF022732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HJK62113.1"
                     /translation="MTDDKTKMDWKPFLWLGIGMILLAVIGVAAIYISGDTPETLAIN
                     YADPYNWYAEGTNPNAEMDVFYLHGDVDISEIKPHAINVDVTVFEVRQNVHDSILDAV
                     NEYPDEMNAYIPYYRQVTEYAKQSSTAISEKTAEALAYADAKAAFHYYISEENNDRPY
                     VLAGSGQGAKYISMMITEWFSTRPAYLKNLRAVYLDGVLQDEDDVVILLGTEI"
     gene            complement(1629..>1948)
                     /locus_tag="O0X37_03980"
     CDS             complement(1629..>1948)
                     /locus_tag="O0X37_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HJK62114.1"
                     /translation="PAFVETTLKDDAREDPPITKDALDILGIVSAKDYETLKELTQKI
                     ATIIKEELAKKNIELYDIKFEFGKIGDQIVLIDEISGGNMRAYRNGEYILPLDLEKLV
                     LEE"
BASE COUNT          528 a          438 c          503 g          479 t
ORIGIN      
        1 gtacgggacg gattatccat cgatgacatt gtgaatatcc gttcccgaac acttcgcgga
       61 ggtaaagagc tcgacgtcaa ggttccggac gttggaaaag tgcaggagat agcactgtcc
      121 gaaaaaccgc ttgacatcga ggttgcattt accaaaccgg tacagtttca tcgcaacttc
      181 gacgggacgg ttgaaccagt aggaatgtct ggcacaatga aaaagctgga tgtgatcgac
      241 aacgccagag tatcccgcgt tgttgatcgc tgtacttcag acacggatct cagagcaaca
      301 gaagcagcac gcatcctgtt cgaaaacgga acagatgtgt acaagatcac aaatcttctc
      361 accgccggcc ttctcggagt acaaagaaag gttgtcccca cacggtgggc aattactgca
      421 acggatgaca tgatctcctc cgggatgaaa aaggacgtga tccggatgcc ggcacttcct
      481 gagtatcagg tgttctgcgc aacacttcat ggaaacacac tctgtttcct tttgataccg
      541 tccgaggagt ggcggtttga gatgatcgaa cgctggcaga aacattctct gtgggcgggt
      601 gacgaagaga ccgtgattga ggatggagag accggtcttt caaaaagcaa gtattctcct
      661 attggcgggg catactactc ggcacggctt gcagtgcttg agtacctgaa atccatcggc
      721 aggtgtgcaa aagtactctg cgttcgtgac atatccggcg agtactgggc gccgttgggt
      781 acctgggtta ttcgcgaagc atcacacgta tccctgagaa cagcacccat acgggtgggt
      841 actctgggag aagctaccac tgctgcggct gccggtcttc gaacgaactt ctggattccg
      901 tatgcaaaaa tgctgaaaga catcaaacaa cagaaaacac ttgcggactt cttttcatga
      961 cagacgataa aaccaaaatg gactggaaac catttctctg gctgggaatc ggtatgattc
     1021 ttcttgcggt gattggtgtt gctgcaattt acatctcggg ggacacaccg gagacacttg
     1081 cgatcaacta tgcggatccg tacaactggt acgcggaagg cacgaatccc aatgcggaaa
     1141 tggatgtgtt ctatctgcac ggggatgtgg atatttctga aatcaaacct catgcaatta
     1201 acgtggacgt caccgttttt gaggttcggc agaatgtgca tgactcaata ctggatgctg
     1261 tgaacgaata cccggacgag atgaatgctt acattcccta ctaccgtcag gtgactgaat
     1321 atgccaaaca atcctccaca gctatttcag aaaaaacagc agaagcactg gcatatgcgg
     1381 atgcaaaagc ggcgttccat tactatatat cagaggaaaa caatgatcgc ccatatgttc
     1441 ttgcaggatc ggggcaggga gcaaagtaca tctcaatgat gatcaccgaa tggttttcca
     1501 cacgccctgc atatctgaaa aatctgcgcg cagtgtatct ggacggagtt ttacaggatg
     1561 aagatgatgt ggtaattctg cttggaacag agatctaaaa aataaaaatg agtttagagg
     1621 gaagagtgtt attcttccag gaccagtttt tcaaggtcaa gcgggagaat gtactcaccg
     1681 ttgcggtacg cacgcatgtt tccgccggag atctcgtcga tgagaacaat ctgatcgccg
     1741 atttttccga actcgaattt aatgtcgtaa agttcgatgt tcttcttggc aagttcctct
     1801 ttgatgatag tggcaatttt ctgggtaagc tcttttaagg tttcatagtc ttttgcagaa
     1861 acaatgccaa gaatgtcaag tgcgtctttg gtaatcggcg ggtcctcgcg tgcgtcatcc
     1921 tttaaggtcg tctcaacaaa tgcgggga
//
LOCUS       DALUAJ010000188         4736 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_36706,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000188 DALUAJ010000000
VERSION     DALUAJ010000188.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4736)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4736)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4736
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36706"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="O0X37_03985"
     CDS             complement(<1..864)
                     /locus_tag="O0X37_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62115.1"
                     /translation="MVETIGWRHVNNLSGLDMTTQDLLITRISDQIREEQDYTKILEK
                     GGLRLARTKYWVPVPVLLFLIWAVVCITNGISALTILMLWIVGSFFIYMFYFFLMMIP
                     SLGSDSSSHLTSTHTQIQIREFLRLANLLKTVRKNKVTFIEIFWNAFLINAKPLAKGF
                     AIIYLIDMVCAVILYAEEMIDLHLLIIVCFQILLIFAFYAKIVRSKPDTPGFFVPRNQ
                     ENVSPETETAKIKIWLYISLFAMITGLLVVGAMLFPGITLNNYLLVISILPSEYPMIL
                     ILVLLVQAVIIR"
     gene            875..1810
                     /locus_tag="O0X37_03990"
     CDS             875..1810
                     /locus_tag="O0X37_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HJK62116.1"
                     /translation="MGADADNTKKQNVARLSVFSNLFLTVAKIITGISIGSVSIISEG
                     IHSGMDLLASCIAYFSVKKSAEPPDEDHAFGHGKFEDASGLIEALLIVLAAGIIIWEA
                     CHKLLTGGGMISLELLYAGMIVMGISAVINFVVSQRLMKVAKETESIALESDAWHLRT
                     DVLTSAGVFAGLVLIQLTHLVFLDSIIAIIVALLILREAYSLIRRSFADLMDESLDED
                     EVILIREIICRHANEFTNFHSLKTRRSGPNRFVEFHLMMPRATPLDAAHAVLKEIENE
                     LVAEMPRTSVIVHLDPCDGRCGKMECSFTCKKAEK"
     gene            1867..3489
                     /locus_tag="O0X37_03995"
     CDS             1867..3489
                     /locus_tag="O0X37_03995"
                     /inference="COORDINATES: protein motif:HMM:NF037981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HJK62117.1"
                     /translation="MDFADRPVYNVGDNVPPSVLVLSILQHFFVLAVYMTYPVIITKA
                     INGGEDLSTFLISATLIGSGIATILQAIRYTGCGYIFPMVPNSSYLPASMLAATAGGL
                     PLLYGMMIVSGFLEMILSRLTRYFRVLFPGEVVGVVMFMLGLAIIPFSFPLFFGSGDT
                     GPLDPAATAVGIITLGSMIILGIQHQKIFRFYAVLIGIIIGFASSVLFGVLTLADIES
                     AGSTSLFSIPNPVGIVSYSFDVGLLIPFAIGMLCVMLKSAGNIALLDEYTGETGRGNL
                     RRGILSEGFGAAVCSAIGGIGIGSSASNTGLIPGTGIASRKIGLGLGLFLIICGFLPA
                     IGWFFHIMPEPIMGAVVIYAIAFIMLGGVQSISSRVLDNRRTFVVILPIMIGVSSVMC
                     PNLYTALPETIQLFFASPLTSGAIFVVVLGLLFKIGLPTHNTIRFGDHPEADVSRLLL
                     DCGRLWTMDKTQMFQIIHHIQALIRAMPEGMHPEQLTLTLKNSTGALTAELIVPGPVD
                     ETAMKSAGHYPATVTIATDAGKTIIRSGYLMI"
     gene            complement(3521..3718)
                     /locus_tag="O0X37_04000"
     CDS             complement(3521..3718)
                     /locus_tag="O0X37_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62118.1"
                     /translation="MTIESSIDRLVIGMMVSAIVIGLSRVLMTQPAEAGYYPLLAYIS
                     AIIVIVIILYKMKTRKFKREE"
     gene            complement(3696..>4736)
                     /locus_tag="O0X37_04005"
     CDS             complement(3696..>4736)
                     /locus_tag="O0X37_04005"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HJK62119.1"
                     /translation="ALKIQRPNIQERIEQDIVLLRSLAERIEERRPDLRIYNPVALVD
                     DFSVQIKKELDFTRDGMNAERLARNMRVSGIPGIKVPKIYWEFSGKCLLAMEFVSGVR
                     SDDVDAIVAMGMDPKELASRGLKAFMVQIFQNGFYHGDPHAGNIRVSPKGELVFLDFG
                     VCGVLMKDTRNKFISLMLALFAADADLTIRYVKNLGVKIPSSGMDEFRGELYLALQDS
                     KDMGAQMNFSGLVAAIQELFQRNNIRVPPNLMQLLKALMLVSNIAFTLDPKLEFSKEA
                     EPYLKKLIAEDMKNPENVQKKILEAKVRLEDLSRVPQQLSAVLEMASEGKLRIDCSKR
                     GERTPYDDREFH"
BASE COUNT         1050 a         1238 c         1171 g         1277 t
ORIGIN      
        1 ccggatgata accgcctgta ccagcagtac aaggataaga atcatcggat actcgcttgg
       61 aagaatggag atgaccaaga gatagttgtt gagtgtaatt cccggaaaca gcattgcccc
      121 cacaaccaga agcccggtga tcatggcaaa gagactgatg tagagccata tcttgatctt
      181 tgccgtctct gtttccgggg atacgttctc ctgattccgg gggacaaaga atcccggcgt
      241 gtcgggtttt gaccggacaa tttttgcata gaatgcaaag atgagcagaa tctggaaaca
      301 gacgatgatc agcaggtgca gatcgatcat ctcttctgca tacaggatga cggcacagac
      361 catatcgatg agatagatga tggcaaatcc ctttgcgagc ggttttgcat tgatgagaaa
      421 tgcattccag aagatctcga tgaaggtaac cttgtttttc cggaccgttt tcagcaggtt
      481 ggcaagccgc aggaactcgc ggatctgaat ctgggtatgc gtactggtaa gatgacttga
      541 tgaatcagat ccaagagacg ggatcatcat gaggaagaag tagaacatgt agatgaaaaa
      601 actcccgacg atccacagca taagaatagt cagcgcggaa atcccgtttg taatacacac
      661 gaccgcccag ataaggaaga ggaggaccgg taccggaacc cagtattttg ttctggcaag
      721 tctgagccct cccttttcga ggattttggt gtaatcctgc tcttctctga tctgatctga
      781 gatgcgggta atcagtagat cctgggttgt catgtctaga ccgcttaagt tatttacgtg
      841 ccgccaccct atagtttcta ccattgtgag cggtatgggc gcggatgcag ataataccaa
      901 gaaacagaat gttgcccgtc tgtcggtgtt ttccaatctg tttctcacgg ttgcaaaaat
      961 tatcaccggt atctcgatcg gttcggtgag tattatttcg gaaggtatcc attcgggaat
     1021 ggatctgctt gcatcctgta ttgcatactt ttcagtgaaa aagtcggcgg agcctccgga
     1081 cgaggatcac gcgttcgggc acggaaagtt tgaggatgca tctggtctta tcgaagcact
     1141 gctgatcgtc cttgcggcag gcatcatcat ctgggaggca tgccacaaac ttctcaccgg
     1201 cggggggatg atctcgcttg agctgctgta tgcaggtatg attgtaatgg gaatctctgc
     1261 tgtgatcaac ttcgttgtgt cgcagcggct gatgaaggtt gcaaaggaga ccgagtcgat
     1321 tgcactggag agtgatgcct ggcatctgcg tacggacgtt ctgacctctg ccggagtgtt
     1381 tgcgggtctg gtcctgattc agcttactca tctggtgttc cttgattcga tcatcgcaat
     1441 cattgtagct ctgctgattc ttcgtgaagc atactccctg atccgcagaa gttttgcgga
     1501 tctgatggat gaaagtctgg atgaggatga ggtgattctc atccgggaga tcatctgccg
     1561 ccatgcaaac gagtttacca acttccacag tttgaagacc cgcaggtccg gaccgaaccg
     1621 gtttgttgag ttccatctga tgatgccgcg tgcaactccg cttgatgcag cccacgcagt
     1681 cctcaaagaa attgaaaacg aacttgttgc agagatgccg aggacttcag tgattgtgca
     1741 tctggacccc tgcgacggac gctgcggaaa gatggagtgt tcgtttacgt gtaagaaggc
     1801 ggaaaaataa tttttgcctc tctccaaact ttctaatatc cgccagacaa acctatctct
     1861 ttaattatgg actttgcaga ccgtccggtg tacaatgtcg gcgacaatgt tcccccgtcc
     1921 gttcttgtcc tgagtattct tcagcatttt tttgttctcg ccgtttacat gacataccct
     1981 gtcatcatca ccaaggcaat caacggcggc gaggatctct cgacgtttct tatcagtgca
     2041 acccttatcg gttccggtat tgctacgatt cttcaggcaa tccgctacac cggctgcggg
     2101 tacatcttcc caatggttcc caactcctcc tatcttcctg catccatgct tgccgcaacc
     2161 gcaggaggtc ttcccctcct ttacggtatg atgatcgtct ccggatttct ggagatgatt
     2221 ctcagccggc ttacgcggta cttccgtgtg ctcttccccg gtgaggttgt tggcgtcgtc
     2281 atgttcatgc tgggtcttgc aatcattccg ttctcgttcc cgcttttctt cggcagcggc
     2341 gatacagggc cgctcgatcc cgcggcaact gcggtcggca tcatcactct cggctcaatg
     2401 atcatccttg ggatccagca tcagaaaata ttccggttct atgcggttct tatcggcatc
     2461 atcattggtt ttgcctcttc tgttctcttt ggggttctca cgcttgcaga catcgagtcc
     2521 gcaggatcga cgtctctgtt ctccatcccg aaccctgtcg gaattgtcag ctacagtttt
     2581 gatgttggtc ttctcattcc gtttgccatc gggatgctct gtgtcatgct gaagtccgcg
     2641 ggaaacatcg cacttcttga cgagtacacc ggtgaaaccg gccgtgggaa tctccgtcgc
     2701 gggatcctct ccgaagggtt cggggctgct gtctgttctg ctataggcgg tattggaatc
     2761 gggtcgtctg catccaacac cggacttatc cccgggaccg gtattgcttc ccgtaaaatc
     2821 ggtcttggtc ttggtctctt cttaatcatc tgcggatttc ttcctgcaat cggctggttc
     2881 ttccacatca tgcccgaacc aatcatgggg gcagtcgtca tctatgcgat cgccttcatt
     2941 atgctgggag gtgtacagag catctcatcc cgtgtcctgg acaaccgccg gacctttgtg
     3001 gtgattctgc caatcatgat tggcgtctcc tctgtcatgt gcccgaatct ttacaccgca
     3061 cttcccgaga ccattcagct tttctttgca tcccccctta cctccggggc gatctttgtg
     3121 gttgtgcttg gtcttctctt taaaatcggt cttccaacac acaataccat cagattcggg
     3181 gatcatccgg aagctgatgt ctcccgtctc ctgcttgact gcggacgcct ctggaccatg
     3241 gacaagaccc agatgtttca gatcattcat catatccagg cactcattcg tgccatgccg
     3301 gagggtatgc atcctgaaca actgaccctc accctcaaaa acagcaccgg tgcccttact
     3361 gccgaactca tcgttccagg tccggtcgat gaaactgcca tgaagtctgc ggggcattat
     3421 cctgcaactg ttaccattgc aactgatgca ggaaaaacga tcatccgttc cggctatctt
     3481 atgatctgat ggaatgtccg gaatctgtat aaaaaaagag ttactcttcc cgcttgaact
     3541 tgcgggtctt catcttgtag aggatgatca caatcacgat gattgcggag atgtaagcca
     3601 gcaacgggta gtatcctgcc tcggcgggct gggtcatcag cacccgggag agaccgataa
     3661 caatcgccga gaccatcata ccgatcacca gccggtcaat ggaactctcg atcgtcatac
     3721 ggagttcgct cacctctttt gctgcaatct attctgagct ttccttccga cgccatttcc
     3781 agcactgcgg acagctgctg cggtacacgg gagagatcct ccagccgcac ctttgcctca
     3841 agaatcttct tctggacgtt ttccggattt ttcatgtcct ccgcgatcag cttcttgagg
     3901 taaggctcag cctcttttga gaactcaagc tttggatcca gcgtgaaggc aatgtttgat
     3961 acaagcatca gtgcttttag gagctgcatc aggtttggcg gaacccggat gttgtttcgc
     4021 tggaacagct cctgaattgc tgcaacaaga ccggagaagt tcatctgggc acccatgtct
     4081 ttgctgtcct gcagggcaag atacaactca ccgcggaact catccatacc gcttgacgga
     4141 atctttaccc cgagattttt tacgtaccgg atcgtcagat ccgcatctgc tgcaaagaga
     4201 gccagcatca gcgagatgaa tttgttccgg gtatccttca tcaaaacgcc gcagacgcca
     4261 aaatcaagaa acactagttc gccttttggg gatacacgga tatttcctgc atgcgggtca
     4321 ccgtggtaaa atccgttctg gaagatctgg accatgaatg ctttcaggcc ccggcttgca
     4381 agttctttcg ggtccatgcc cattgcaacg atcgcatcca cgtcgtcact tctgacacct
     4441 gaaacgaact ccattgcaag cagacacttt ccggagaact cccagtaaat ttttggcact
     4501 ttaataccag ggatgccgga tacccgcata ttccgtgcaa gccgctcggc attcattccg
     4561 tcacgggtga agtccagttc ctttttaatc tgtaccgaga agtcgtccac cagggcaacc
     4621 ggattgtaga ttctgagatc cggtctccgt tcctcaatcc gctcggcaag ggatcggagg
     4681 agaacaatat cctgttcgat tcgctcctga atgttcgggc gctgaatctt tagggc
//
LOCUS       DALUAJ010000189         2522 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_37345,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000189 DALUAJ010000000
VERSION     DALUAJ010000189.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37345"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(85..1722)
                     /locus_tag="O0X37_04010"
     CDS             complement(85..1722)
                     /locus_tag="O0X37_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HJK62120.1"
                     /translation="MLRYGAERAGDDRPALAYYGNHISYGQLLADVHACAAGLRAHGV
                     KRGDFVTIFLPNIPQCVVAVYAVNLIGAVCNMVHPLSTTGETGYAVDLTESKYVLTCE
                     INEAVCSGMDVEIIRCRTPSYFPKTLKGYLMGWMYRRAVRKCLNGENVRKITEWDDLL
                     AEGRAAVAASGIPEMVGSPDDTAVIMYTGGTTGTAKGVMLSNFAVNTVAVQLVVGIGG
                     EDICVGDGFLAILPMFHAFGLAVTAHTPLSAGLKVVLLPRFNAKECAHAILSEKTAYI
                     AGVPAMYERMYSEFEGKDLSFIKLIVAGGDSVSGSLINRYNQLLRQGNCMVKFRPGYG
                     LTEACSVCALTEDGYRTVPTGCVGIPLMGNKVCTVEPGTVNVLPDGEEGELCLFSGAV
                     MTGYYRNQEASEAVLRRHEDGNIWLHTGDIVKITPNGDICFRSRYKRMVKVNGYNVYP
                     MMIEEVMQTHPDVKEVCAIGIPWKHDTRIKLYVTLKRKMDPAEAERKLIEYAMGRLNR
                     WSIPAAVEIVASLPRTKMEKTDYRALEELELRKNASR"
     gene            complement(1838..>2522)
                     /locus_tag="O0X37_04015"
     CDS             complement(1838..>2522)
                     /locus_tag="O0X37_04015"
                     /inference="COORDINATES: protein motif:HMM:NF016030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase large subunit PriL"
                     /protein_id="HJK62121.1"
                     /translation="GIGKMPVFKYVELASTVREPKWRLINRDVDGGMVTISEDELDVL
                     LRERIRKILVSQLPLTIPPSMEHEFVPWCEKITAALQERTLAEFGAIDESAYPPCIQA
                     LIAGAAAGVNLSHSGRFAMTAFLNNIGMTPNQVAGIFARSPDFNPDMTMYQVDHITTH
                     EYTTPACQTMLTHGICVNRDSLCDKIAHPLNYYRAKKKLLERKRLRQEAQALADAKKT
                     VSEKPEKQL"
BASE COUNT          612 a          697 c          598 g          615 t
ORIGIN      
        1 atctgaccgt tgccggtctg actgttatca aaaagacaat cacctaaaac atcagggaaa
       61 aaaatatttt tttgctgttt agactcagcg ggatgcgttt ttacggagct ctaactcctc
      121 cagcgcacga taatctgttt tttccatttt tgtacgcggg agtgaggcaa caatctcgac
      181 tgcggcggga atactccacc tgttcagtct tcccattgca tactcaatga gcttgcgctc
      241 cgcttctgcc ggatccatct tccgcttcaa tgtgacatac agcttgattc tggtgtcatg
      301 cttccaggga ataccaattg cgcagacctc cttgacgtcg ggatgcgtct gcatgacctc
      361 ctcaatcatc atggggtata cattgtagcc gttcaccttt accatgcgtt tgtaccgtga
      421 gcggaaacaa atgtcaccgt tcggagtaat ctttacaata tccccggtgt gcagccagat
      481 atttccatcc tcatgcctcc tgagaacagc ctcgcttgcc tcctggttcc gatagtaccc
      541 ggtcattact gcaccgctga acaggcaaag ctccccctcc tctccgtcgg gcagaacatt
      601 gactgttccc ggctcaaccg tacagacttt gtttcccatc agcggaatac cgacacaacc
      661 ggtgggaaca gtacggtatc catcctccgt cagtgcacag acactgcatg cctcggtcag
      721 cccgtacccg gggcggaact tcaccataca gttcccctga cggagaagct ggttgtaccg
      781 gttgataaga ctgccgctga cactgtcgcc gccagcaacg atcagtttga tgaacgagag
      841 atcctttcct tcaaactcac tgtacatccg ctcgtacatt gcgggaaccc ctgcaatata
      901 tgcggtcttt tccgagagaa ttgcatgcgc acactctttt gcattaaacc tcggcagaag
      961 aacaaccttc agaccggcag aaagcggtgt atgggcagta acggcaagac cgaatgcgtg
     1021 aaacatcggg agaatagcca ggaatccgtc acccacacaa atatcctcac cgccgatacc
     1081 gacaacaagc tgaacagcga cggtattaac cgcaaagtta gagagcatca caccttttgc
     1141 tgtcccggtc gttccgccgg tgtacataat aactgcggtg tcgtcaggtg atcccaccat
     1201 ctcaggaata ccggatgcag caaccgcggc acgcccttct gcaagaagat catcccactc
     1261 ggttattttg cggacgtttt caccgttgag gcacttccgg actgcacggc ggtacatcca
     1321 ccccataaga tagcctttca gggttttggg gaagtaggat ggggttctgc aacggataat
     1381 ctcgacatcc attcctgaac agaccgcttc gttgatctca caggtcagca catacttgct
     1441 ttcggtcaga tcaacggcat atccggtctc accggtggtt gacagcgggt gaaccatgtt
     1501 gcagaccgcg ccgatcagat tgaccgcata gacggcaaca acgcactgcg ggatgttagg
     1561 aagaaagatg gtaacaaaat caccacgctt aaccccgtga gcacggagac cagcagcgca
     1621 ggcatgaaca tcagcaagaa gctgtccgta actgatgtgg ttcccataat aggcaagagc
     1681 tgggcggtca tctcctgctc gctctgctcc gtatctgagc atagaataca gggtttgttt
     1741 cgctgagtca ggatgaatta ctactggcac gttgttcctc cacaccccac agtttactca
     1801 tatgatgtat cggcggtgtc tataaatatt tacgggatta cagctgtttc tccggctttt
     1861 ccgaaacagt tttttttgca tcagccaatg cctgtgcctc ctgccggaga cgttttcttt
     1921 ccagaagttt cttctttgca cgatagtagt ttaagggatg ggcaattttg tcacacagag
     1981 aatcacgatt cacgcagata ccatgtgtca gcatggtctg gcaggcaggc gtcgtgtact
     2041 cgtgtgtggt aatgtgatcc acctgataca tggtcatatc gggattaaaa tcaggactcc
     2101 gggcaaatat tcctgcaacc tggttcggag tcataccaat gttgttcaga aacgctgtca
     2161 ttgcaaacct tcccgagtgg gagaggttaa caccggcagc agccccagca atcagtgcct
     2221 gaatgcacgg aggatatgct gactcatcga tagccccaaa ctctgcgagt gttctctcct
     2281 gcaaagcagc ggtaatcttt tcgcaccagg gcacaaactc atgctccatc gacgggggaa
     2341 tggtgagagg gagctgcgac acaaggattt ttctgatgcg ttcccgcagc aggacatcca
     2401 gttcatcctc ggatattgta accatcccgc catccacatc acggttgata agacgccact
     2461 tcggctcacg gacggtcgag gcaagttcca catatttgaa caccggcatc ttcccaatgc
     2521 cc
//
LOCUS       DALUAJ010000190         3260 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_37493,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000190 DALUAJ010000000
VERSION     DALUAJ010000190.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37493"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(18..1760)
                     /gene="oadA"
                     /locus_tag="O0X37_04020"
     CDS             complement(18..1760)
                     /gene="oadA"
                     /locus_tag="O0X37_04020"
                     /EC_number="7.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-extruding oxaloacetate decarboxylase
                     subunit alpha"
                     /protein_id="HJK62122.1"
                     /translation="MTKLYITDTTLRDAHQSLAATRMRTEDMLELAAAMEKAGFWSVE
                     AWGGATFDTCIRYLNDDPWERLRDLKKIFVKTPIQMLLRGQNLVGYKHYPDDVVDKFI
                     AAAAKNGVDVFRVFDALNDIRNMQRSMTAVLNEGKHLQGTISYTTSPVHSTEGFIDMA
                     EELYSLGCNSICIKDMAGLIMPEAARALITGIKERIDIPVCLHTHSTSGIAPMSYQAA
                     IEAGVDILDTAMSPFAGGTSQPATESIVASLAGTKRDTGIKLTTLRDIKNAAAAVRCK
                     YDCLIDPISVRIDSDVLIYQLPGGMISNLVSQLKEQNALDKMDEVLKEIPVVRKDLGY
                     PPLVTPTSQIVGTQAVLNILMGGRYKTVTNEVRDYLKGMYGKSPAPLDDALVSSIIGS
                     EERISCRPADTLGPIYDKMKADAESQGLIKKDEDVLTYILYPAIAPAFLKGEKKAEPL
                     PEAAPATAVAAAPVDIDIPRSMEVEVDGEVFMVRILSVEGESVAAGSSEPGKRQTPHG
                     DVPGGVKSSIQGMVLDIKVQMGQKVSAGDTLLVLEAMKMENPVVSPVDGTVTEIFVEN
                     GAVVQSGDALVGVK"
     gene            complement(1892..2539)
                     /locus_tag="O0X37_04025"
     CDS             complement(1892..2539)
                     /locus_tag="O0X37_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62123.1"
                     /translation="MTDIRIRLTVVILLSLTAFTGVLGTLLAAASWLLFCADETFSHN
                     SWKIFVPSAVLAAGFPGLILYLTGGDGMIYAAKIFVIFCFAFWLGVSHKPGEFLDLGV
                     WALGRKTGFDLGLSAELTMQYLSGISDDLSHMKSALKIKGEKLTRKTIPPLATGLLLL
                     SLSRADQIGAHLARRGYRMGGTYLPHFTTTKTDILMLCTAGVCAAAVLSSSIPAL"
     gene            complement(2532..3098)
                     /locus_tag="O0X37_04030"
     CDS             complement(2532..3098)
                     /locus_tag="O0X37_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJK62124.1"
                     /translation="MKLIFDAVRFQRDHFTLSVDAAFSCGIHLISGRIGTGKSTLALA
                     AAGILSPNSGEILYEEIMDTPLLLMQFPEYQVTGTTVSEEITSWNITGNEEPFHRLNT
                     GPSSRDPLTLSRGELRRLELACILSRRSDLLILDEPYASLDQAAKPVLTGLLEQRTGI
                     TLIFSHETEYLPTTAEHWTIQEGKLHHD"
BASE COUNT          725 a          815 c          896 g          824 t
ORIGIN      
        1 gaagtagcgg tctttgttca tttcaccccc acaagcgcgt cgccgctctg gacaaccgca
       61 ccgttctcca caaagatctc cgtgaccgtt ccgtcaaccg gactgaccac cgggttttcc
      121 atcttcatgg cttcgagcac gaggagtgtg tcgccagcag agactttctg acccatctgt
      181 accttgatgt cgagcaccat tccctgaata ctgctcttca caccgcccgg cacatctccg
      241 tgaggtgtct ggcgcttgcc gggttcagag cttcctgcgg caacagattc tccttcgaca
      301 gagaggattc tgaccatgaa cacctcaccg tcaacctcga cctccattga gcgggggatg
      361 tcaatgtcaa ccggtgctgc tgcgactgct gtcgcgggcg ctgcttccgg caggggttct
      421 gccttcttct cgcctttcag gaatgccggg gcgattgccg gatagaggat gtatgtcagc
      481 acatcttcgt ccttcttgat aagaccctgg ctttcggcgt ctgctttcat cttgtcgtag
      541 ataggaccaa gcgtatctgc cgggcggcag ctgatccgct cctcactgcc gatgatcgag
      601 gagaccagtg cgtcatccag cggtgccggg gattttccgt acatgccttt cagataatcg
      661 cggacctcgt tggttacagt cttgtaacgt cctcccatca gaatattgag aactgcctgg
      721 gttccgacga tctgtgaggt tggggttacg agcggcgggt agccgagatc ttttctgacc
      781 accgggatct ctttgagtac ctcgtccatc ttatcaagag cgttctgctc ctttaactgg
      841 gatacaaggt tggagatcat acctcccgga agctgataga tcaggacatc actgtcgata
      901 cggaccgaga ttggatcgat caggcagtcg tatttgcacc ggacggcagc tgcggcattc
      961 ttgatgtcac gaagtgtggt cagcttaatg cctgtatccc gtttggtacc ggcaagagat
     1021 gcgacaatac tctccgttgc gggctgtgat gtacctccgg cgaacgggga cattgcagtg
     1081 tcgaggatgt caacgcctgc ctcaattgct gcctgatagc tcatcggcgc aattccgctg
     1141 gttgagtggg tgtgcagaca tacggggatg tcgatccgct ctttgatacc agtaattaat
     1201 gcacgggcag cttcaggcat gatgagtcct gccatgtctt taatgcagat cgagttacag
     1261 ccaaggctgt acagctcctc ggccatgtcg ataaaccctt cagtactgtg aacggggctt
     1321 gtcgtgtaac tgattgttcc ctgcaggtgt ttaccttcat tcaggacggc agtcattgag
     1381 cgctgcatat tgcggatatc gttgagcgca tcaaacacac ggaagacatc aaccccattc
     1441 tttgcggcgg cggcgatgaa cttgtcaacc acatcatccg gataatgctt gtagccgaca
     1501 aggttctgac cgcgaagcag catctgaatg ggagtcttga caaagatctt tttcagatcc
     1561 cgtaaccgct cccacggatc atcgttcaga taccgaatgc aggtgtcaaa tgttgctcct
     1621 ccccaggctt caacagacca gaagcccgct ttctccatcg cggcagcgag ttcaagcatg
     1681 tcttcggtac gcattcgtgt tgctgcgagc gactggtgcg catcccgaag ggttgtatca
     1741 gttatataga gctttgtcat ggttgtctcc cggttctgca ttgtgagtgc agtttatcta
     1801 aacctatact gttttggaat gaaaaggtaa aaaagtagtc ctgattgatc cggtccggaa
     1861 ctgcgtgtcc ggatactatt tatatgatgt atcacaatgc cgggatggag gaagagagaa
     1921 cggcggcagc gcagactcct gcggtgcaga gcatgaggat atccgttttt gttgtggtaa
     1981 aatgcggcag ataggttcca cccatgcggt atcctctcct tgcaagatgt gcgcctatct
     2041 gatccgcacg ggagagggag aggagaagaa gtcctgttgc gagcggcggg atggttttgc
     2101 gggtgagttt ttctcccttg atcttcagcg cggatttcat atgggagaga tcgtctgaga
     2161 tgccggagag gtactgcatg gtaagttcgg cggaaaggcc gaggtcaaac cctgttttcc
     2221 tgccaagtgc ccagaccccg agatcaagaa actctcccgg tttgtgtgat acacccagcc
     2281 agaatgcgaa acagaatatg acaaagatct ttgctgcata gatcatgccg tcacctccgg
     2341 tgaggtacag aataagtccc ggaaaccccg ctgcaagaac tgcggaggga acgaatattt
     2401 tccatgagtt gtgggagaat gtttcatctg cacagaataa tagccaactg gccgcagcaa
     2461 ggagtgttcc aagaactccg gtgaatgcgg taagggagag gagaataacc accgtgagac
     2521 gtatgcggat gtcagtcatg atgcagtttt ccctcctgaa ttgtccagtg ttccgctgtt
     2581 gttggcaggt actcagtctc atgagaaaat atgagggtga tgccggtacg ttgttcaagc
     2641 agtccagtga gtaccggttt tgcggcctga tccagcgagg catagggttc atcaagtatg
     2701 aggagatcac ttctccgcga aagtatgcag gcgagttcca gtcggcgaag ttctccgcga
     2761 gataaggtga gtggatcacg ggaggatggt ccggtgttca ggcgatgaaa gggttcctcg
     2821 tttccagtaa tgttccagga ggtaatctct tcagataccg tggttccggt cacctgatac
     2881 tcgggaaact gcatcaatag cagcggcgta tccatgattt cttcgtatag gatctctcct
     2941 gagtttggag agagaattcc tgctgctgca agtgcgagtg tggattttcc ggtaccaatc
     3001 ctccctgaga tgagatgaat gccacaggaa aatgctgcat ccacggatag tgtaaagtga
     3061 tcccgctgaa accgtactgc atcaaaaata agtttcacag atcactcacc ttcccgtaca
     3121 cgagtgtgat ccggtgatct gctctttctt ggacgctgcg tgagtgtgta ctccagagta
     3181 catacgggca gccgctgttt ctgatgatct gaaacagttc atctgtggtc tgcggatcaa
     3241 gatgggaatc gggctcgtca
//
LOCUS       DALUAJ010000191         2834 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_37648,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000191 DALUAJ010000000
VERSION     DALUAJ010000191.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37648"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(94..708)
                     /locus_tag="O0X37_04035"
     CDS             complement(94..708)
                     /locus_tag="O0X37_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 subunit beta"
                     /protein_id="HJK62125.1"
                     /translation="MVDSYEDMLKAAYSGMTEPTSTGERFVMPKTKVYIEGKTTVLEN
                     FADIAGTLNRDKDHFMKFILGELGTAGKIDGTRGVFNGKFEESQFEAIVTTYVNDYVI
                     CSECGRPDTKLVKDDRVLMLLCEACGSKRPIRKRKTKTEVQGPAIEEGKELEVQIESI
                     SKKGDGVAHVGKYILYVSGAKAGQTVKVRITRISGSVAFTQKIL"
     gene            805..2829
                     /gene="purL"
                     /locus_tag="O0X37_04040"
     CDS             805..2829
                     /gene="purL"
                     /locus_tag="O0X37_04040"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HJK62126.1"
                     /translation="MLAEHDEEYIRSVLGRNLKPLEAAAFEKVWSEDCSYRITRKLLD
                     TLPTIGGSVILGPGDDAAIVKFSDELSLAIAMGSQNRSSCTNPYQGAATGVGSVVRDV
                     LSMGAQPIGIQTPWFFGDISDEKTQWIIRNATAGAADYSNTINVPVLPGYQMFDESFR
                     GNPLINMICFGVMKPNRFMFGKAFKTGWKLILFGRTTGRAGSSDVEGEPQIEANLISA
                     TLEMFEKKTILACRGLGTTGICGASADMCVDVGVRIFADAIPLHAEEMSTEEILFSET
                     QERMLAEIHPDKVEEAKEICAKYGISCVVVGETTGSDRYVVEFGGEVVCDLPIKLLAE
                     GAPEKEYPTKPYTAEKPFVRPAGTIRELALKVLSHPDLADNSMHATQFNSQAQGRTYA
                     ATPFYSVLELEGNGLSLACGCNARHTYLDPYTGAANTILELASHIVSVGGIPFCALDS
                     LNFGSPENPEIMWQISEIVKGIGDMCRVLHIPIAGGNVSLYNESDTCDTTIKPAAAVA
                     MFGKGDLIGWEWPDVGDSIAIVGETRAELGGSVLDAVTGCGGTVPELGSVAALPVIQD
                     LVEKAQVSGSMAITRGGLLYALVNLAAQAKVTIRGDAVTKLFSETYGRFLVTFSDEHF
                     LKESGLSYEIIGKITADGKLTIQTEEEEVVLTQQDLFMAGSTITRACRAR"
BASE COUNT          710 a          607 c          816 g          701 t
ORIGIN      
        1 atcccgaaga gtttgcaaat acggtactgg aattttttgg gacgtaagag tttttcaaaa
       61 aaaagtgtac cgggagattc cggaacagat attttagaga attttctggg tgaatgcaac
      121 ggagccggag attctggtga tccgtacttt gacggtctga ccggcttttg cccctgagac
      181 atacaggata tatttgccga cgtgggcaac gccgtctccc tttttgctga tggattctat
      241 ctgaacttcg agttcttttc cttcctcgat cgccggaccc tggacttcgg tctttgtctt
      301 acgctttcgg atcgggcgtt tggatccgca tgcttcgcag agaagcatca gtacacgatc
      361 gtctttgacg agttttgtat ccgggcggcc gcactcggaa cagatcacat agtcgtttac
      421 gtaagtggtg acaattgctt caaactgtga ctcttcgaac tttccattga agacaccgcg
      481 ggtaccgtcg atcttgccgg cggttccaag ctcacctaag atgaacttca tgaagtggtc
      541 tttgtcacgg ttgagtgttc cggcgatgtc ggcgaagttt tccaggacgg tggtcttacc
      601 ctcaatatag accttggtct tgggcatgac gaaccgttct ccggtgcttg tcggttccgt
      661 catacctgag tatgccgcct tcagcatatc ctcatatgaa tcaaccatac tcctatattt
      721 tagcgctgga ggcttaaagt tctggcgtca cccggataca tacaggtgtt tttctttgca
      781 gcgatcaaat agtttggata agttatgctg gcagaacatg atgaggagta tattcgatcc
      841 gttctcggac ggaatctgaa accgctggaa gcggctgcat ttgaaaaagt gtggagtgaa
      901 gattgtagct accggattac aaggaagctt ctggatacac tgccgacaat aggaggaagt
      961 gtaattctcg gacccggaga tgatgcggcg atcgtgaagt tctcggacga actttccctg
     1021 gcaattgcaa tgggatcaca aaaccgttca agttgtacaa atccatatca gggggcagca
     1081 acaggagtgg gaagtgttgt tagagatgtg ctttcgatgg gggcgcaacc tatcggaata
     1141 cagactccct ggttctttgg agacatttca gacgaaaaaa cgcagtggat aattcgtaat
     1201 gcaaccgcgg gagctgcgga ttacagtaac acaatcaatg tgccggttct tccgggatac
     1261 caaatgtttg acgaaagttt cagaggaaac ccgctgatta atatgatctg tttcggggtg
     1321 atgaaaccga accggttcat gtttgggaaa gcgttcaaaa ctgggtggaa gctgatcctc
     1381 tttggcagaa caacaggacg ggccggttct agcgacgtag agggagagcc ccagattgag
     1441 gcaaatctta tctcagcaac actggagatg tttgaaaaga agacgatcct tgcatgtcgg
     1501 ggtctgggga cgacaggaat atgcggggca tctgctgata tgtgcgtgga tgtcggtgta
     1561 agaatttttg cagatgccat acctctgcat gcggaagaga tgagcacaga agaaattctg
     1621 ttttccgaaa cgcaggagag aatgcttgca gagattcatc ccgacaaagt ggaggaggca
     1681 aaggagatct gtgcgaagta tggtatttca tgtgtggtgg tcggcgagac gacaggatct
     1741 gatcggtatg tggttgagtt cggcggggaa gtggtgtgtg atctgccgat aaaactcctt
     1801 gcggagggtg cgcccgaaaa ggagtacccc acaaaaccct atacggcgga aaagccgttt
     1861 gtccggccag caggaacaat ccgggagctt gccctcaaag tgttgtccca tccggatctt
     1921 gcggacaaca gtatgcacgc aacccagttc aattcacagg cacaggggag aacttatgcg
     1981 gctaccccgt tttactcggt actggagctg gaagggaacg gactctctct tgcctgcggg
     2041 tgtaatgcca ggcataccta tctggatccg tacactggtg cggcaaacac aattctggaa
     2101 ctcgcatccc acatcgtctc agtcggaggg atcccgttct gtgctctgga cagtttaaac
     2161 ttcggttcac cggaaaatcc ggagatcatg tggcagattt cagagattgt gaagggtata
     2221 ggggatatgt gccgggtgct gcatattcca attgcagggg gaaatgtttc actgtataat
     2281 gagtctgata cctgtgacac tacgataaaa cctgccgcag cggttgcaat gtttggaaaa
     2341 ggagatctga tcggctggga gtggccggat gtgggagaca gcatcgcaat tgtgggagag
     2401 acaagagcag agcttggagg atcagtcctg gatgcggtaa ccggatgcgg cggtactgtt
     2461 cccgaactcg gaagcgttgc ggcattgccg gtcatccagg atttggtcga aaaagctcag
     2521 gtaagcggtt cgatggcgat tacacgggga ggactgctgt atgcgctggt gaatcttgca
     2581 gcccaggcaa aggtaacgat ccggggagat gcagtaacaa aactgttctc agaaacatac
     2641 ggacggtttt tggtgacgtt ttcggatgag catttcctca aagagagcgg actttcctat
     2701 gagattatcg gaaaaattac ggcggacgga aaactgacga ttcagaccga agaggaggaa
     2761 gtagtactga cacagcagga tctcttcatg gcgggaagta cgattacgcg ggcctgtcgt
     2821 gcacggtgag atac
//
LOCUS       DALUAJ010000192         3033 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_37788,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000192 DALUAJ010000000
VERSION     DALUAJ010000192.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37788"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="O0X37_04045"
     CDS             <1..537
                     /locus_tag="O0X37_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HJK62127.1"
                     /translation="ALSLLYEHVKADSLRGHCIATAAVMKGLAEELSEDAGLWETIGI
                     LHDIDFEEIGEDMTRHGLAGYDILKAAGVGDEIAEPIKRHNHMLFSGEYTTPVEICLQ
                     VADSVSGLVIACAYVKGGKITDVTPKTVTKKYKTASFAAGCDRNRIALGDALVPRERM
                     YEIAIREISAVRDQLGLA"
     gene            540..1052
                     /locus_tag="O0X37_04050"
     CDS             540..1052
                     /locus_tag="O0X37_04050"
                     /inference="COORDINATES: protein motif:HMM:NF021625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62128.1"
                     /translation="MSAKEQSVILKALQQAHGRVMNIPRYLLPSGGVALAYAVANPRD
                     IKDIAVVRSNGIGFGTDDTLVRVLLTVVRFDAEVRAVGCIRYTEEIADVVRETLRDVA
                     EYDPSKFPPGISSMDWGIASCCRDGVPLVIIDSGTKDTEPLIRILGTTPEEVANRILI
                     ISERLTYTDI"
     gene            1063..1257
                     /locus_tag="O0X37_04055"
     CDS             1063..1257
                     /locus_tag="O0X37_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17e"
                     /protein_id="HJK62129.1"
                     /translation="MGIKPSYIKTMGTALMEQHGASFNNNFDDNKRVVSEVTTIESKI
                     IRNRVAGFITRKVNTNNRRR"
     gene            1460..2353
                     /gene="dapA"
                     /locus_tag="O0X37_04060"
     CDS             1460..2353
                     /gene="dapA"
                     /locus_tag="O0X37_04060"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HJK62130.1"
                     /translation="MFEGVIPALITPFREDPSQSLDIEGLRSCIEHVISGGVHGLLPC
                     GSTGESATMTHAEHIRVIEEAVSAANNRIPVIAGTGSNNTEEALLMTRAAKIAGADGA
                     LLISPYYNKPNRSGLLKHYKKVADVGLPVVVYNIPGRTGQNLPPDLIIEMAKTIPNIV
                     AVKEASGNIDQMMAIIHGLEGVEREYPFVLTSGDDSLTLPVLSIGDQGCISVTANIEP
                     KRMVAMYDAFVHGDMDKARKIHYELMELSKALFMDVNPVPAKRAAEIRGLIASGNVRL
                     PLDSLNPQMTEKLREVLSHYD"
     gene            2346..>3033
                     /gene="dapB"
                     /locus_tag="O0X37_04065"
     CDS             2346..>3033
                     /gene="dapB"
                     /locus_tag="O0X37_04065"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HJK62131.1"
                     /translation="MTKVMICGALGRMGTTIANMVVENPDLELVGGVDIRPGQILGKP
                     VVESSCLAAFIEEQKPDVMIDFTVAAATMVNAKIAAAHGVALVIGTTGFTPEQDAELL
                     DAVKGVPVVKTTNFSVGVNIFWELVREAAKRLGDYDIEVIEAHHRHKKDAPSGTAKTI
                     LKVLEEEVGKREEMYGRCGMTERGNEIGVHVIRGGDVVGDHTVQFHQNYETIELTHRA
                     YDRAVFARGAV"
BASE COUNT          708 a          770 c          858 g          697 t
ORIGIN      
        1 gcgttatccc ttctgtatga acatgtaaag gcggattcgc ttcgcggcca ctgtattgcg
       61 accgctgctg tcatgaaggg acttgccgag gagctttcgg aggatgcagg tctgtgggag
      121 acgatcggta ttctgcatga cattgatttc gaagaaattg gcgaggatat gacccggcac
      181 ggccttgctg gatatgatat tttaaaggcc gccggagtcg gcgatgagat tgcagagccg
      241 atcaaacgcc acaatcatat gctgttttct ggtgagtata caactccggt cgagatctgt
      301 ctgcaggttg ccgacagtgt ctccggtctg gtgattgcat gtgcctacgt caaaggaggc
      361 aaaattacgg atgtcacgcc aaagacggtg acaaagaagt acaaaacggc ctcttttgct
      421 gcgggatgcg accggaacag aattgccctt ggcgatgcgc ttgttccgcg tgagcggatg
      481 tatgagattg caattcgcga gatttccgcg gtacgggatc agctcgggct tgcataatta
      541 tgtcggcaaa agagcagtct gtaatactga aggctcttca gcaggcccac ggtcgtgtga
      601 tgaatattcc acgctatctc cttccttccg gcggggtggc acttgcgtat gcagtagcaa
      661 atccccgtga cataaaagac atcgcggtag tcaggtcgaa cggaatcggg tttggtaccg
      721 acgacacgct tgtccgcgtg ctgctgacgg tcgtcagatt tgacgccgaa gtccgcgcgg
      781 ttggatgtat ccggtacacc gaggagatcg cggacgttgt cagggaaacg ctgcgtgatg
      841 ttgccgagta cgatccgtca aagtttccac cggggatctc gagtatggac tgggggattg
      901 cctcctgctg ccgtgacggt gtccctctgg tcattatcga cagcggaacc aaggataccg
      961 agccgctgat ccggattctt gggaccactc ctgaagaggt tgcaaacaga atccttatca
     1021 tatctgaacg acttacatat acagacatct gaggaatatc gcatgggaat taagccaagc
     1081 tatatcaaga ctatgggcac tgccctcatg gaacagcacg gagcaagctt caacaacaac
     1141 tttgacgaca acaagagagt tgtttccgag gtcaccacca ttgagagtaa gattatccgc
     1201 aaccgtgttg ccggattcat tacccgcaag gtcaatacca acaaccgccg ccggtaaatt
     1261 atcacccccg caaatactcg tgcgggatgt gtccgttccc ccgtggggtt ccgcctcccg
     1321 gcgaaaaaac atttttttct gtctgtactg taccggatag ttttcaggat cagcgcatca
     1381 tactgccacg gtcagttcgg ttttttgtgc atgtccaagc gaagttacat gtagctggtg
     1441 cgtcctacgt ataagtacta tgtttgaagg tgtgattcct gcactcatca ctccgttccg
     1501 tgaggatcct tcacagtctc ttgatattga gggactccgg agctgtattg agcatgtgat
     1561 ctccggaggc gttcacggtc ttctgccctg cggctcgacc ggcgagtcgg caacgatgac
     1621 acatgccgaa cacatccgtg tcattgaaga agcggtatcc gcggcaaaca accgtattcc
     1681 tgttattgcc ggaaccgggt ccaacaacac tgaggaggca cttctcatga cccgcgccgc
     1741 aaaaattgcc ggagcagacg gcgcccttct gatcagcccc tactacaata agccaaaccg
     1801 gagcggtctt ctcaagcatt acaaaaaggt cgcagatgtc ggtcttcccg tggtggtgta
     1861 caacatcccg ggcagaactg gtcagaacct gccgccggac ttaatcatcg agatggcaaa
     1921 gaccatcccc aacatcgttg ccgtcaaaga agcaagcggc aacattgatc agatgatggc
     1981 aatcattcac ggtcttgagg gtgtggagcg cgagtatccg tttgttctta cctccggtga
     2041 tgattcgctg acgcttccag tgctttccat cggcgatcag ggatgtatct cagttaccgc
     2101 aaacattgag ccgaaacgga tggttgccat gtatgatgcc tttgtgcacg gcgatatgga
     2161 caaggcacgc aaaatccact acgagctaat ggagctgtcc aaagcactgt tcatggatgt
     2221 caaccctgtt ccagccaaac gtgcggcaga gatccgcggc cttattgcct ccggcaatgt
     2281 ccgcctgccg ctggactcgc tgaatccgca aatgaccgag aaacttcggg aggtgctttc
     2341 ccactatgac taaagtaatg atctgcggcg cacttggccg catgggcaca accatcgcca
     2401 acatggttgt ggaaaatccg gaccttgaac tggtcggcgg tgttgatatc cgtcctgggc
     2461 agattctggg aaaaccggtg gtggagtcgt cctgccttgc tgcattcatc gaggagcaga
     2521 agccggatgt gatgatcgac ttcacggttg ctgcggcaac catggtgaat gcaaagattg
     2581 cggctgccca cggtgtggct cttgttatcg gaacgaccgg ttttacgccc gagcaggatg
     2641 ccgagctgct tgatgctgtc aagggtgtgc cggttgtgaa gacgacgaac ttcagcgtcg
     2701 gtgtcaacat cttctgggaa ctggtgcgtg aagctgcaaa gagacttggc gactatgaca
     2761 ttgaggtaat cgaggctcac caccgccaca agaaggatgc tccgagcgga accgccaaga
     2821 cgattctgaa ggttcttgaa gaagaggtcg gcaagcgtga ggagatgtac ggccggtgcg
     2881 gcatgaccga gcgcggaaat gagataggtg ttcatgtaat ccgcggcggg gatgttgttg
     2941 gggaccatac cgttcagttc caccagaact acgagacgat cgagctgaca caccgggcat
     3001 atgaccgggc agtgtttgcc cgcggtgcgg tca
//
LOCUS       DALUAJ010000193         5791 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_37792,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000193 DALUAJ010000000
VERSION     DALUAJ010000193.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37792"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="O0X37_04070"
     CDS             complement(<1..599)
                     /locus_tag="O0X37_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HJK62132.1"
                     /translation="MTSDAVLIDGYVDEPACLGVPPYISPYIRTVAGVFAEHGITTDY
                     VTIDQLRRDPLRVATLSSYKYVVMIAGVTVPGKYLSGTPATLTELQQIGFTLRGRSVS
                     LLGGPIGFGYSPQGGAKAVAQAVSGWTAMLHGDPAAALDAYLSGGEPDGVCRYQDFDR
                     WAVLGAEIIRKHPFYPFVMCELETAKGCSRAVSGGCSFCT"
     gene            complement(589..1218)
                     /locus_tag="O0X37_04075"
     CDS             complement(589..1218)
                     /locus_tag="O0X37_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HJK62133.1"
                     /translation="MKAEEWEPYYSEILDFFGFSREDDEHAAHVLAELLPRDDIAVLR
                     ETISGQDCIVCGNAPSLADEIRKTDFSGKVIIAADAAARVLFHAGHRPDVILSDIDGM
                     DDDFLVMNDLGTILVLHAHGDNIPLVRSWVPKVRGPIVGTTQATPLAHVYNFGGFSDG
                     DRCVFCAYELGAARVSLIGFDLDDRSVDPVKHGKLMIARKLLKLCGHDI"
     gene            complement(1208..2698)
                     /locus_tag="O0X37_04080"
     CDS             complement(1208..2698)
                     /locus_tag="O0X37_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HJK62134.1"
                     /translation="MVESDGVLPNNNQTHKKIKYLILGCGSIGYNVLEELKEDDENVL
                     VIDVNEKRVEDLRDHRHQAMVGDMTDPGLLAKIPEPEMVFILAADKDANLAAVQNIKG
                     AYPQTHVIARAVDLFSADQLVDHGADVVLYPQQVVAKSAVNHMNNLVASRNAQKLFSL
                     LSSWSGTLGIITHKNPDPDAISSAMALSAIAANASGGKLATRILYEGNIGHQENRAFV
                     NLLEIKMERLTPEILGECNYLALVDCVAPGMNNDLPLDTPINIVIDHHSTEGVVRLRK
                     PEYLDSRPNIGATASIMTQYLQELYLPIDKKVATALFYGIRADTREFQRNLSPQDLHN
                     AAYLLPLSDRSLLEVIMAPSVSQETLDVLGHAIVNREVRHGYLFSNVGYVRNRDAIPQ
                     AADMLLNLEGVSTAMVYGITDTSITLSARNKDIRLHVGDVMKEAFSPIPGASAGGHAT
                     MAALSIPLNAFSLVKNKDELLNMIIDPVLSNFMRIVGISEAEGDES"
     gene            2819..2892
                     /locus_tag="O0X37_04085"
     tRNA            2819..2892
                     /locus_tag="O0X37_04085"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2853..2855,aa:Lys,seq:ctt)
     gene            2921..2994
                     /locus_tag="O0X37_04090"
     tRNA            2921..2994
                     /locus_tag="O0X37_04090"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2955..2957,aa:Lys,seq:ctt)
     gene            3177..4937
                     /gene="thrS"
                     /locus_tag="O0X37_04095"
     CDS             3177..4937
                     /gene="thrS"
                     /locus_tag="O0X37_04095"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HJK62135.1"
                     /translation="MKVIYNDGKVGECPKEEELHVIRHTAAHVLAQAVKRLYPHASFA
                     YGPATEKGFYYDVDLGDTKLSEADLPAIEQEMREIVKANLPIKPFILSRADALKLMEE
                     RHEPYKIEHIADLDENEPLSFYEQGEYIDMCVGPHLTYTKTLKAFKLTGLSGAYWKND
                     SKNKMLTRINGTAFATTKELEDFLKLQEEAERRDHRKIGKDMKLFMFEDVGPGFPFFL
                     PNGMIIKNELIEYWRELHTANGYQEISTPLIMNRCLWETSGHWDHYKENMYATRIDDE
                     DYCIKPMNCPGGVMVYANEPHSYRELPMRLAELGIVHRHELKGVLHGLFRVRCFTQDD
                     AHIFMRQDQIAEEISSVIRLIHQVYSKFGFEYTLELSTRPEDSMGSDEDWEAATNGLV
                     NALEQLNLPYTINEGDGAFYGPKIDFHLRDCLGRTWQCGTIQLDFQMPINFNLSYVDE
                     NDERPRPIMIHRVCFGSIERFIGILTEHFAGKFPVWLAPMQAKVLLVSKKSEAYATEI
                     YQMLKDAKIRCELDNRNEKIGYMIRQAQYTERVPYMIIIGEKELETKTISVRTRDSKT
                     VSMTPEEFLAKITTEIANRD"
     gene            complement(4956..5228)
                     /locus_tag="O0X37_04100"
     CDS             complement(4956..5228)
                     /locus_tag="O0X37_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62136.1"
                     /translation="MTDPIVGDWEKSMMLVGRMHCRFYPDNTGIAIGKVLGHDINEPF
                     QWTCKGNGVYHICAAGYEHDIHLTGDRLETVFRGHALDMARVTCKK"
     gene            complement(5221..>5791)
                     /locus_tag="O0X37_04105"
     CDS             complement(5221..>5791)
                     /locus_tag="O0X37_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJK62137.1"
                     /translation="PAGVRVLECGCGNGKTLAAMRSRGWDVIGIDISPAAVSLASAAL
                     ARGNFSLSDDSTGLHCCSVVCGDVTSLPFSDNSFDAVFCWHVLGHLPATVWPTAASEM
                     WRVLRPSGKIFFKGFSRNDMRCGKGREVAPYSYLRGDGIVTHYFTGTELREVFGTGEL
                     TRVSWKMRVRGTDYLREELCGVFGREGFD"
BASE COUNT         1577 a         1532 c         1406 g         1276 t
ORIGIN      
        1 gtacaaaagg aacatccccc tgaaaccgca cgggaacatc cctttgcggt ctcaagctca
       61 cacatcacaa acggatagaa cggatgttta cggataatct ctgctccaag cactgcccat
      121 cggtcaaagt cctgataccg gcagacacca tccggttctc caccggacag atatgcatca
      181 agtgcggctg caggatcccc gtgcagcatt gcagtccatc cagagacagc ctgcgcaaca
      241 gcttttgctc caccctgtgg agagtaacca aacccaatag gtcccccaag cagtgatact
      301 gatcttcccc gcagcgtgaa acctatctgc tgcagctcgg ttagtgttgc aggtgtcccg
      361 gaaagatatt tgccgggaac ggtgacgcca gcgatcataa ccacgtactt gtaggaggag
      421 agggttgcaa ccctcagagg atcacgccgc agttgatcaa tcgttacata atcagtggta
      481 atgccgtgtt cagcaaacac accagcaaca gttctgatgt acggagagat gtacggcgga
      541 acaccaagac aggcgggctc atccacatac ccgtcaatga gaactgcatc agatgtcatg
      601 accgcaaagt ttcagaagtt ttcgtgcgat catcagtttt ccgtgcttta ccggatccac
      661 cgagcgatca tccagatcaa acccaatcag agagactcga gcagcaccaa gctcataggc
      721 acaaaacaca caccgatccc cgtcggaaaa tccgccgaag ttgtacacat gtgcaagcgg
      781 cgttgcctgc gttgtcccga caatcggccc tcggaccttt gggacccatg accgtacaag
      841 cggaatattg tcaccgtgcg catggagtac cagaatcgtc ccaagatcat tcataacaag
      901 aaagtcgtca tccatcccgt cgatgtcaga gagaattaca tccggacgat gaccggcatg
      961 gaaaagaaca cgcgccgcag catcggcagc aataatgacc ttaccggaaa agtcagtctt
     1021 acgaatctca tcagcaagag acggtgcgtt tccgcaaacg atacagtcct gaccagaaat
     1081 tgtctcacgc agaactgcaa tatcatcgcg gggcagaagc tctgcaagaa catgtgctgc
     1141 atgctcatca tcctcacgtg aaaatccaaa aaagtcgagg atttcagagt agtagggttc
     1201 ccactcctca gctttcatcc ccttcagcct cggagatacc gacaatccgc atgaagttgg
     1261 acagaaccgg atcaatgatc atattgagca gttcgtcttt gttcttcact aaagagaacg
     1321 cgttcaacgg aatagacagt gctgccattg ttgcatgacc tcctgcagat gccccaggaa
     1381 ttggagagaa cgcttctttc atcacatcac cgacatgcag acggatgtcc ttgttgcggg
     1441 ctgaaagcgt gatagaggtg tcagtgatac catacaccat tgcagtactc acaccttcaa
     1501 gattcaggag catatcagct gcctgcggaa ttgcatcacg attgcggacg tatccaacat
     1561 tggagaaaag atacccgtgt ctgacctcac ggttaacgat tgcatgaccg agaacatcca
     1621 gcgtttcctg tgagacagat ggtgccatga taacctcaag aagagatcga tccgacagag
     1681 gaagaagata agcagcgttg tgcagatcct gcggagaaag attccgctgg aactcccggg
     1741 tatctgcacg aataccatag aacagggctg ttgcaacctt tttatcgatc ggcagataca
     1801 actcctggag atactgcgtc ataatggatg cagttgcgcc aatgttaggg cgggaatcaa
     1861 ggtactccgg tttccgcaga cgcactacac cttcggtgct gtggtgatcg atgacgatat
     1921 tgatcggcgt atccagcggg aggtcattgt tcatgccggg agcaacacag tccaccaggg
     1981 caagatagtt gcattcaccg agaatctccg gtgtgagtct ttccatcttg atttcaagaa
     2041 gattaacgaa cgcacggttt tcctgatgac cgatgtttcc ttcatagagg atgcgtgttg
     2101 caagctttcc gccggaagca tttgcagcaa ttgcggaaag tgccattgcg ctggagattg
     2161 catcgggatc aggatttttg tgcgtgataa tgccaagggt cccgctccac gaacttaaaa
     2221 gggagaaaag tttctgggcg tttcttgacg caaccagatt attcatgtga ttgacggcag
     2281 acttagcaac aacctgctgc ggatacagca caacatccgc accgtgatcc accagctgat
     2341 ctgccgagaa cagatccaca gcacgggcaa tcacatgggt ctgcggataa gcgcccttaa
     2401 tgttctgtac tgctgcaagg tttgcatcct tgtccgctgc aagaataaag accatctctg
     2461 gttctggaat cttggcaagc agaccggggt cggtcatgtc gcctaccatt gcctgatgac
     2521 ggtggtcacg cagatcctcc acacgttttt cattcacatc aataacgagg acgttttcat
     2581 catcctcctt gagttcttcg aggacattgt aaccgatact gccgcagccc agaatgagat
     2641 attttatttt tttatgggtc tggttattgt tcggcagcac cccatcactc tcaaccatgt
     2701 accaatattc gtgttgtttg gatattaatg tagataccca cagggcccaa aatcccctcg
     2761 tgaagaaatc tataaatatt gatatgacta atctaattag gtcaacagac atcacaaagg
     2821 gcctatagct tagtcaggaa tagcgacgga ctcttaatcc gtaggccagg ggttcaaatc
     2881 cctttaggcc cgcttttggt gtgagttgaa tgtttttact gggcctatag cttagtcagg
     2941 aatagcgacg gactcttaat ccgtaggcca ggggttcaaa tccctttagg cccgctttta
     3001 gtgcagattt gagaaacaag gtccaaggca tataccagag cctttcgtga ccaaaataca
     3061 tatcctgtaa aaaaccaaaa catgtacggt tatcttgggc tgcacagaac tcgcctttgc
     3121 agcgtacaag aggatttgta tatccgctga aacggaaaac agcaggaact acttgcatga
     3181 aagtaattta caatgacgga aaagtgggcg agtgcccaaa agaagaagag ctgcatgtta
     3241 tacgccacac cgcggctcat gtacttgccc aggcagtgaa acgtctctac ccccacgcat
     3301 ccttcgccta cggccctgca accgagaagg gattttacta tgatgtcgat ctgggagaca
     3361 cgaaactttc ggaagccgac cttcctgcca ttgaacagga gatgcgggag atcgtcaagg
     3421 caaatctgcc gataaaaccg ttcatcctct cacgtgcaga tgcattaaaa ctcatggaag
     3481 aacggcatga accatacaag atcgaacata ttgcagacct tgatgagaat gaaccgctga
     3541 gtttctatga acagggcgaa tacatcgata tgtgcgtcgg tccccacctg acctatacaa
     3601 agaccctgaa agcattcaaa ctgaccggcc tttccggcgc atactggaaa aatgacagca
     3661 aaaacaagat gctgacaaga atcaacggaa ccgcctttgc caccaccaag gaacttgagg
     3721 atttcctgaa actgcaggag gaggcagagc gtcgtgatca ccgcaagatc ggcaaagaca
     3781 tgaaactctt catgttcgag gatgttggtc caggcttccc gttcttcctg ccgaacggaa
     3841 tgatcatcaa gaacgaactg atcgagtact ggcgcgagct gcacacggcc aacggatatc
     3901 aggagatctc aacgccgctc atcatgaacc gctgtctctg ggagacaagc ggtcactggg
     3961 atcactacaa agagaacatg tatgcgaccc gcatcgatga tgaggactac tgtatcaaac
     4021 cgatgaactg ccccggcggt gtgatggtgt atgcaaatga gccgcacagc taccgcgagc
     4081 tgccgatgcg tcttgccgag ctgggaattg tgcaccgtca tgaactgaaa ggtgttctgc
     4141 acggcctctt ccgggttcgc tgtttcacac aggatgatgc ccatatcttc atgcgccagg
     4201 atcagatcgc cgaggagatc agttctgtaa tccggctgat tcaccaggtg tactccaagt
     4261 tcggctttga gtacactctt gagctctcca cccgcccgga ggacagcatg ggaagtgatg
     4321 aggactggga agcagcaaca aatggtctgg taaatgcact ggagcagctg aatcttccct
     4381 acacgatcaa cgaaggcgac ggtgcattct acggcccgaa gattgacttc catctgcgcg
     4441 actgtcttgg acgtacctgg cagtgcggaa ccatccagct ggatttccag atgccgatca
     4501 acttcaactt atcctatgtg gatgagaacg acgaacggcc gcgcccgatc atgatccacc
     4561 gcgtatgttt cggatcgatc gagcggttca tcggtatcct gaccgaacac tttgccggaa
     4621 agttcccggt ctggcttgca ccaatgcagg caaaagttct gctggtctcc aagaagagcg
     4681 aggcatatgc aaccgagatc tatcagatgc tgaaagatgc aaaaatccgc tgcgagctgg
     4741 acaaccgcaa tgagaagatc gggtacatga tccgccaggc ccagtacact gagcgtgtgc
     4801 cctatatgat catcatcggc gaaaaagagc ttgagacaaa gactatctca gtccggacgc
     4861 gggacagcaa gacggtgtct atgacgcccg aagagttcct ggcaaagatt acaaccgaga
     4921 ttgcaaaccg ggactaacct cccgaaaata tttttttatt ttttgcaggt gacccgcgcc
     4981 atgtcaagtg catgcccgcg gaacaccgtt tccagccggt cacctgtgag atgaatgtcg
     5041 tgttcatacc cggctgcaca gatatgatag acaccgtttc ctttgcatgt ccactgaaac
     5101 ggttcattga tatcatgacc gagaactttg ccaatggcaa taccggtgtt atcgggataa
     5161 aaccggcagt gcatacgtcc gacaagcatc atcgactttt cccagtcacc aacgattgga
     5221 tcagtcaaat ccttccctcc cgaatacacc gcagagctct tcgcgcagat agtccgtccc
     5281 gcggacacgc atcttccatg aaactctggt tagttctcct gtgccaaaaa cttcccggag
     5341 ctcagtgccc gtaaaataat gcgtgacaat cccgtcaccc cgtagatacg agtacggtgc
     5401 aacctcccgg ccttttccgc accgcatatc gtttcgagag aatcccttga agaaaatctt
     5461 cccagacggc cggagaacac gccacatctc cgatgctgct gtaggccata ccgttgcggg
     5521 gagatgaccc agaacatgcc agcagaatac cgcatcaaaa gagttgtcag aaaacggcag
     5581 tgaagtaaca tccccgcaaa caactgaaca acagtgcaaa cctgtgctgt catcagagag
     5641 ggaaaaattt ccccgtgcaa gagccgctga tgcaagggat acagctgcgg gggaaatatc
     5701 gataccaatc acatcccagc cccgcgatct catcgccgca agagtttttc cgttaccgca
     5761 gccgcactca agaacgcgca ctcccgcagg a
//
LOCUS       DALUAJ010000194         1555 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_38338,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000194 DALUAJ010000000
VERSION     DALUAJ010000194.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38338"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="O0X37_04110"
     CDS             complement(<1..560)
                     /locus_tag="O0X37_04110"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HJK62138.1"
                     /translation="MLTAVGLGPGDAELLTLRAVRLLSEADTVFVPGGIAQDLVKPYA
                     KKIVALEFPMTRDEEVITACMERNAEKIVPVAKNGKAVFGLIGDPNFYSTFSRLAEMV
                     RARYPGLEVTTVPGISSITAVASHAEIPVNGAFLVTDGPDVPATKILMKVTHPREKAE
                     MLKTEGYNDFIVVERMYMDGECVHRGA"
     gene            complement(554..1087)
                     /locus_tag="O0X37_04115"
     CDS             complement(554..1087)
                     /locus_tag="O0X37_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJK62139.1"
                     /translation="MELPGGPTQPEVMAVSLEKLGVQPGDTVADIGCGTGTVTKAIAA
                     LAGTCGHVYAVDRRPAAVSITRDTCAAFPQVEVFEGEALSFLSGPHKQVNCAFCGGSR
                     DIAEVITRLDKEGCRSIVVNAVLIETAVETIHVMKELGIFREAVHLQVSRSYELTERI
                     MFKPINPIYIIHGGREC"
     gene            complement(1247..1462)
                     /locus_tag="O0X37_04120"
     CDS             complement(1247..1462)
                     /locus_tag="O0X37_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HJK62140.1"
                     /translation="MSVESMQDGRVRRNIHVGSAVAIVLKKDQPTGALTWGRVAEILT
                     NSPSHPHGIKVRLSDGSVGRVQQIYDK"
BASE COUNT          429 a          442 c          328 g          356 t
ORIGIN      
        1 gctccgcggt gcacacactc gccatccata tacatccgct ccacaacgat gaagtcattg
       61 tagccttcag tcttgagcat ctctgctttc tcacgaggat gcgtaacctt catcaaaatt
      121 tttgttgccg gaacatcagg accatcagtc acaagaaatg caccgtttac aggaatctcg
      181 gcatgcgatg ccacagcggt aattgagcta atacccggaa ccgtggtaac ttcaagaccc
      241 ggataccgtg cacgtaccat ctcggcaaga cgagaaaacg tcgaataaaa attcggatcc
      301 cctatcagac caaaaaccgc cttgccgttt tttgccacag gaacaatttt ctcagcattg
      361 cgttccatac atgccgtgat aacctcctca tcacgcgtca ttggaaactc aagggccaca
      421 attttcttgg catatggttt caccagatcc tgggcaatac ctcccggcac aaacacagtg
      481 tccgcctcag aaagcaaacg gacagctcta agggtcagaa gctctgcgtc accagggccg
      541 agaccgactg ctgtcagcat tcccgccctc catgtataat atagatggga ttgattggtt
      601 tgaacataat acgctccgta agttcatagg aacgagacac ctgcagatga accgcctccc
      661 ggaaaatacc cagctccttc atgacatgga tagtctcaac agcggtttcg ataagcactg
      721 cattaaccac aatactcctg cacccctctt tatccagccg ggtgataacc tcagcaatgt
      781 cacgactccc accgcagaaa gcacagttca cctgtttatg aggaccagag agaaacgaca
      841 gtgcctcacc ctcgaacacc tctacctgcg gaaaggcggc acaagtatca cgcgtaatcg
      901 aaacagccgc aggtctgcgg tcaacagcat atacatgccc gcaggtaccc gcaagtgcgg
      961 caattgcttt tgtcactgtg ccagtaccgc agccgatatc ggccacagta tcgcccggtt
     1021 gtacaccgag tttttcaagt gataccgcca tcacttccgg ttgtgtaggt cctccgggta
     1081 gctccatact aatctctctt cttaggaaaa attgtcttag cgaaagttgc taagtagtat
     1141 ttgtgaggga gagttattag ttgtatgcag ttcgtccggg aaaagcaaag atcgcaccat
     1201 cccacgaaaa ccccattccc caacaaaagg gttcccgaaa aagaggctat ttatcatata
     1261 tctgctgcac acggccaacc gatccatccg aaagccggac ctttattccg tgcggatgag
     1321 agggtgagtt tgtcagaatc tcagcaacac gtccccaggt aagagcacct gtcggctgat
     1381 cttttttcag aacaatcgca actgccgaac caacatgaat attcctgcgt acccgtccat
     1441 cctgcatact ctcaacactc attcgaatcc cggctataaa agttcatcat ctctgaggtg
     1501 aaacatgaaa aaacacacat ggcaaatgag tttgaacgcg ccgtcgttat ctgca
//
LOCUS       DALUAJ010000195         3626 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_38416,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000195 DALUAJ010000000
VERSION     DALUAJ010000195.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38416"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="O0X37_04125"
     CDS             <1..977
                     /locus_tag="O0X37_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2791 family P-loop domain-containing protein"
                     /protein_id="HJK62141.1"
                     /translation="ARICAGLTTQTEDHALKSIIDTWIYGIEDRLISTGIDENDAALA
                     DMTSKEIEAVLTRVSGINSGLAAALRTYYKANNSGDFATAQAAIGWISGEQTVGRSFK
                     AQAGLKGTVTEGDALAFLNGLVHIIVQAGYAGLLVSFDEVETAQAFARPQREKGYINL
                     RQIVDMIDRNELPNCFFLFAGTPALFDSAKGIRSLPPLYDRIGMIADDGFSNPMQTQI
                     VLPKFDVKKLEEVSLRVIDIYTEAYSEVDKTRVSIRFIRTMIDRVTGRFGGRVDVVPR
                     LYLREFVDVLDKCALYDSYNPMEKYAFVAKENDTSLKEEEKAVMEVSW"
     gene            1034..1975
                     /locus_tag="O0X37_04130"
     CDS             1034..1975
                     /locus_tag="O0X37_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HJK62142.1"
                     /translation="MDKNLYVKDITNGSDVSSLFLIKRSDLRSKRGEQFVHMTLADRT
                     GTIECKAYGNKAVEAARAVKTGMVYAISGVGKINQNGELHVFSDSPETLDSLLAGPVT
                     VMPGQETEFLFTPADIKGNAAEIQKVVASIGNSDLRLFVACCLDHRFYECPAAIRRHH
                     EYLGGLCEHSLETVRIALNLVETMPRTEINRDMVIAGAVLHDIGKIFCFDKVGYGYVP
                     NDTYNLIEHITMGIMFIDEYKSLISESQLLQLRHIIQSHHGIYGDVVPLTAEAWLVNQ
                     ADGASSMLRSIVDDLTETPKGQKKKGLRSEKFLWKAV"
     gene            2186..2881
                     /locus_tag="O0X37_04135"
     CDS             2186..2881
                     /locus_tag="O0X37_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048061969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HJK62143.1"
                     /translation="MHIMEGYLPIEWCVLWAVLSAPCVIYGLWRMKQMIQQDRRVLPL
                     MAVCGAFVFVLSALKIPSVTGSCSHPTGTGLSASFFGPWVTAVLGTIVLLFQAILLAH
                     GGLTTLGANVFSMAIAGPFVAWLVFVALRRYAHAGLGIAVFVTAAVANLVTYSITSLQ
                     LALAFPADGSVFAAFAAFAAIFAVTQIPLAVIEGIICGLVAKYIVRVKPDILKKLGII
                     EDAEIAKIQEEAA"
     gene            2878..3177
                     /locus_tag="O0X37_04140"
     CDS             2878..3177
                     /locus_tag="O0X37_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HJK62144.1"
                     /translation="MNRATTEIIIGIAVIVIFVIGTLFLIPSGGDDGEGWGGADGGAA
                     DLIDSTGYVPWFNPIWEPPSGEIESLFFCVQTAIGAVIVGYFFGYWRGAKGRKEE"
     gene            3322..>3626
                     /locus_tag="O0X37_04145"
     CDS             3322..>3626
                     /locus_tag="O0X37_04145"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62145.1"
                     /translation="MITFLIGIAILILGYCIWGKVAEKIFAPDDRLTPALANPDSVER
                     VPLSKKRNMLLQLLNIAGMGPIVGVALGMKFGAIVFLLLPIGNVIGGAIHDYYIGMIS
                     "
BASE COUNT          885 a          855 c          943 g          943 t
ORIGIN      
        1 atgcaagaat ttgtgccggt cttaccacgc aaacggagga tcatgccctc aaaagcatca
       61 tcgatacctg gatctacggt atcgaagacc ggctgatcag taccggcatc gacgagaacg
      121 atgcggcact tgccgatatg acctcaaagg agatcgaagc ggttctcacc agggttagcg
      181 gaataaactc gggtcttgca gcagcacttc gtacctacta caaagcgaat aacagcggtg
      241 actttgccac tgcacaggca gcaataggat ggatttccgg agaacagacc gtgggaagat
      301 cctttaaggc ccaggcagga ctgaaaggga cggtcacgga aggagacgcc cttgcattcc
      361 tgaacggtct ggtgcatatt atagtgcagg ccggatatgc ggggctgctt gtcagtttcg
      421 acgaggtgga gactgctcag gcatttgcca gaccgcaacg ggagaaagga tacatcaatc
      481 tccgtcagat cgtggatatg atcgaccgga atgagttgcc gaactgtttc ttcctgtttg
      541 ccggaacgcc ggcactcttt gacagtgcaa aaggtattcg atcacttcct cctctgtatg
      601 atcgaatagg aatgattgcc gatgacggat tctccaatcc catgcagaca cagattgttc
      661 ttccgaagtt cgacgtgaaa aaacttgaag aggtttctct gcgggtgatt gacatctaca
      721 ccgaagcata ctccgaggtt gacaaaacgc gtgtctccat ccggtttatc cgtacaatga
      781 ttgatcgggt aacaggaaga tttggcggcc gcgtggatgt ggtgccgcgc ctgtacctgc
      841 gggagtttgt ggatgtgttg gacaaatgtg cactgtacga ctcctataat ccgatggaga
      901 agtatgcatt tgttgcaaaa gagaatgaca cctcgttaaa agaggaggaa aaagctgtca
      961 tggaagtcag ctggtgagag attcctacaa tcctttattt tttcactgcc tataaatatc
     1021 cacggaacta tccatggata aaaatctcta tgtaaaagat attacgaacg gatcagacgt
     1081 atcgtctctt ttcctcatca aacgttcaga tctccgcagt aaacgcggtg aacagtttgt
     1141 tcacatgact cttgcagatc gtaccggaac aattgagtgc aaagcctacg gcaataaagc
     1201 agtggaggct gcacgggcgg tgaagaccgg aatggtgtat gcaatatcag gtgttggcaa
     1261 gatcaatcag aacggagagt tgcatgtctt ttcggactct ccagagacgc ttgattcact
     1321 tcttgcagga cctgttaccg tgatgccagg gcaagagacc gagttcctgt tcacgcctgc
     1381 ggatatcaaa gggaatgcag ctgagattca gaaagttgtt gcaagcattg gaaacagtga
     1441 tctcaggctg tttgttgcct gttgtctgga tcaccggttt tatgaatgcc cggcagcaat
     1501 ccgccgtcac catgaatacc tcggcggtct ttgtgaacat tcccttgaaa cggtacggat
     1561 agcgctgaat cttgtggaga caatgcccag gaccgagatc aaccgtgaca tggtgattgc
     1621 cggtgcggtg ctgcatgata ttggaaagat cttctgcttt gacaaagtcg gttacggata
     1681 tgtgcccaac gatacctaca accttatcga gcatattacg atgggaatta tgtttattga
     1741 tgagtacaag agtctcatct ccgagtcaca gcttttacag ctccgccata ttatccagtc
     1801 acatcacgga atctacggcg atgttgtgcc tctgactgct gaggcgtggc tggtgaatca
     1861 ggcggacggg gcgagttcga tgctccgcag cattgtggat gatctgaccg agacaccaaa
     1921 gggtcagaag aagaagggtc tccggagtga gaagttcctc tggaaggctg tctgatctcc
     1981 ttttcttttt caggtacata cggcctccat tttccgataa aaactatttt tccgcagcag
     2041 tactggtctg atactccatc attgtgcgct gcatcggtac tgaatggctc gtccttataa
     2101 gatcacacaa aacaaattat ttttctaata taaattataa tttgatttca taacagataa
     2161 ataatagtta gaacaagtat actttatgca cattatggaa gggtatttgc ccatcgaatg
     2221 gtgtgtgctg tgggctgtgc tctcagcacc atgtgtcatc tacggcttgt ggaggatgaa
     2281 acagatgata cagcaggaca gacgcgttct ccctctgatg gcagtctgcg gtgcgtttgt
     2341 gtttgtactt tccgcactca agattccgtc agtgacagga agctgttcgc atcccaccgg
     2401 aacaggtctg tcagcttcat ttttcggtcc gtgggtcacc gcagttcttg gaaccattgt
     2461 attgttattt caggcaattt tacttgcgca cggcggtctt accacgcttg gagcaaacgt
     2521 gttctcaatg gcaatcgcgg gtccgtttgt tgcctggctg gtctttgtcg cactgcgccg
     2581 gtatgcccat gcaggcctcg gtattgcagt gtttgtcact gcagcagttg caaacctggt
     2641 aacttactcg ataacttccc tgcagctcgc ccttgcattc cctgctgacg gaagtgtttt
     2701 tgcagcgttt gcagcatttg cggccatctt tgccgtgacc cagattccgc ttgcagtcat
     2761 tgagggtatc atctgcggtc ttgttgcaaa gtatattgtc cgtgtcaaac ctgacattct
     2821 caagaagctc gggatcatcg aggatgctga aattgcaaag attcaggagg aggcagcatg
     2881 aaccgggcga cgacggagat catcatcggc attgccgtga ttgtgatctt tgtgattggg
     2941 acgctgttcc tgattccttc cggaggagac gatggggagg gctggggcgg tgcagacgga
     3001 ggagccgccg atctgatcga cagcaccggg tatgttccct ggttcaaccc aatttgggaa
     3061 ccgccaagcg gggagattga gtcactgttc ttctgtgtgc agaccgcaat cggtgcagtg
     3121 attgtcgggt acttctttgg atactggagg ggagcaaaag gccgcaaaga agagtaagtg
     3181 agtacacacg tggacgaaaa gccgaacggc ttttcctact ccggggctgg acaccccgtc
     3241 tcttttttta caacgatgtt ctgaacatca aaaacagatc acctggttta tcgtctctca
     3301 acaccacaca tcttacgaaa catgatcact ttccttatcg gaatcgccat tttgatcctc
     3361 ggctactgca tctggggcaa agtcgccgaa aagatctttg ctcccgacga ccgtctcacc
     3421 cccgcacttg caaacccgga tagtgtagaa cgggtgccac tttccaaaaa acgcaacatg
     3481 cttttgcagc tgctcaatat tgccggcatg ggccctattg ttggcgttgc cctcggaatg
     3541 aagttcggcg caatcgtctt tcttctgctc cccatcggga acgtgatcgg tggtgcaatt
     3601 catgactact atatcggcat gatctc
//
LOCUS       DALUAJ010000196         2907 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_38532,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000196 DALUAJ010000000
VERSION     DALUAJ010000196.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38532"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            83..156
                     /locus_tag="O0X37_04150"
     tRNA            83..156
                     /locus_tag="O0X37_04150"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:117..119,aa:Lys,seq:ttt)
     gene            355..603
                     /locus_tag="O0X37_04155"
     CDS             355..603
                     /locus_tag="O0X37_04155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit H"
                     /protein_id="HJK62146.1"
                     /translation="MTRLNVLLHEMVPDHQIMTPEEVAKLLADYDITEEHLPKMYHDD
                     PAVKACGAKPGMVIRITRKSQTAGEATSYRLVVRRPKK"
     gene            619..2172
                     /locus_tag="O0X37_04160"
     CDS             619..2172
                     /locus_tag="O0X37_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B''"
                     /protein_id="HJK62147.1"
                     /translation="MIDRSVLSGAYFSQEHVARHQLDSFNNFVEYNLQKVVNEQRIVE
                     TEIVNRGKNQEPVWVELGELRVEKPIVREADGSQSKLYPTEARLRNLTYAAPMVLTMT
                     LHQGEKEFEPQETTIGQLPVMIGSKVCNLCGLSDAERTEQGEDPSDPGGYFIVNGTER
                     VLMTLEDLASNKIMTEYTERYNEQIHVSKVFSQFRGYRALVVVEKNKKNLLDVSFPSV
                     AGHLRFADLMRALGLETDEEIVSAVSLSEDILTYMMQNLEESECATSEEGIMYVGKKL
                     APNQTKDYQRKRAEFVLDSYLLPHLNYLIPSHLKPGDESYELYAKDVRVAKAEFLGRM
                     AEACFDLALNKRRIDDKDHYSNKRLKLAGDLMEDLFRISLNRLTRDVKYQLERASMRH
                     RELAIGTVVRADVLTERLIHPLATGNWVGGRTGVSQLMDRVDHMSVISHLRRVISPLS
                     RSQPHFEARDLHPTQWGRICPSETPEGPNCGLVKNFAQLVEISKGIEDEEEMLHIIRS
                     MDIEPIRSE"
     gene            2173..>2907
                     /locus_tag="O0X37_04165"
     CDS             2173..>2907
                     /locus_tag="O0X37_04165"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833534.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates. The beta
                     subunit is part of the catalytic core which binds with a
                     sigma factor to produce the holoenzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit B"
                     /protein_id="HJK62148.1"
                     /translation="MEKKMARVFVDGALFGKVDDAEGFTRNFRQMRRNGQIPTEVNIS
                     YKDYSNEIVINTDRGRARRPLIVVENGVPAVTDEDIEKVRSGEYEFMTLVQQGKIEFI
                     DAEEEDDLYIAVYEEDLTPEHTHLEIDPSLILGIGAAHVPFPEHNASPRVTMGAGMIK
                     QALGFAQSNMKLRPDTRGHMLHYAQVPMVHTQAAELIGSDNRPQGQNFCVAIISYEGY
                     NIEDALIFNKASVERGLGRSHYFRTYE"
BASE COUNT          769 a          744 c          744 g          650 t
ORIGIN      
        1 gtgtagtcat ctggaatgac taaccggtat gtatatatat ttttcaatcc caataatgat
       61 gctaccttcc agaggacaac aagggccagt agatcagtca ggcagatcgt cggactttta
      121 atccgcaggt cgggggttca actccctcct ggcccgctta cacgtcgcca tgggttttta
      181 ggatgtcgtt ggaatcaaca ggacaatcct atttactttt cggcagcgtg tattggcgcg
      241 tccgttggac atctgtacaa gcgtcaataa aaaactctga gacttttatg tggtgagaac
      301 tctgaacaca agcacagaca tccgtatcta tcaagatctt aaattaggag ctctatgaca
      361 cgactaaacg tattactcca tgagatggtt cccgatcacc agatcatgac tccggaagaa
      421 gttgcaaaac tccttgccga ctatgacatt accgaagagc accttcccaa aatgtatcat
      481 gacgatcctg ccgtcaaagc ctgcggagca aaaccgggaa tggtcatccg cattacacgc
      541 aaaagccaga ctgcgggtga agcaacatct taccgccttg tggtcagacg gccgaaaaaa
      601 taaccagagt ggatatactt gattgataga agtgtacttt ccggagcgta cttctcccag
      661 gagcacgttg cccgtcacca gctggattcg ttcaacaact ttgtggaata caaccttcag
      721 aaagttgtca acgaacagcg tatcgttgag acggagatcg taaaccgggg aaagaaccag
      781 gaacccgtat gggttgaact cggagaactc cgagtagaaa agccgattgt ccgtgaagcg
      841 gacggatcac agagtaagtt atacccaacc gaagcacgtc tgcgcaacct tacctatgca
      901 gccccgatgg ttcttaccat gacgctccac cagggcgaga aagagtttga accgcaggag
      961 accacgattg gtcagcttcc ggtcatgatt gggtccaagg tctgcaacct ctgcgggctt
     1021 agtgatgcgg agagaaccga acagggtgaa gatcccagtg atcccggagg atatttcatt
     1081 gtcaacggaa ccgagcgtgt gttaatgacg cttgaggatc ttgcctccaa caagatcatg
     1141 accgagtaca ccgagcggta caatgagcag attcatgtat caaaggtatt ctcgcagttc
     1201 cgcggatacc gtgcgctcgt tgttgtcgag aaaaacaaga agaatctcct cgatgtcagc
     1261 ttcccgtccg ttgccggtca cctccgcttt gcggatctca tgcgtgcctt gggtcttgag
     1321 actgatgaag agattgtgtc cgcagtctcc ctctcagaag acattctgac ctacatgatg
     1381 cagaaccttg aggagtccga gtgtgcaacc tccgaggaag gtatcatgta tgtcggtaag
     1441 aaactcgcac cgaaccagac gaaggattac cagaggaagc gtgccgagtt tgttcttgac
     1501 agctacctcc tgccgcactt aaactacctc atcccgtctc acttaaagcc gggagacgag
     1561 agctatgagc tgtacgcaaa agatgtccgc gtcgcaaagg cagagttcct cggcagaatg
     1621 gcggaagcat gtttcgatct cgccttaaac aagaggagaa tcgacgataa agatcactac
     1681 tccaacaagc gtctgaagct tgcgggagac ctgatggaag atctcttccg tatctcctta
     1741 aacagactta cccgcgatgt gaagtaccag cttgagcgtg cgagcatgcg ccatagagaa
     1801 ctcgccatcg gtaccgtcgt ccgtgcagat gtactgaccg agcggttaat tcacccgctt
     1861 gcaaccggaa actgggtggg cggcagaacc ggtgtgtccc agctgatgga ccgtgtggat
     1921 cacatgtccg ttatctccca cctgcgccgt gtcatctcac cgctgtcccg gtcccagcct
     1981 cacttcgagg cccgtgatct gcacccaacc cagtggggaa gaatctgtcc gtccgagaca
     2041 ccggaaggac caaactgtgg tctggtgaag aactttgccc agcttgttga gatcagcaaa
     2101 ggaatcgagg acgaagaaga gatgctgcac attatccgca gtatggacat tgaaccaatc
     2161 cggagtgagt aaatggaaaa gaaaatggca agggtattcg tcgacggtgc gctgttcggc
     2221 aaggttgatg atgcagaggg atttaccaga aatttccgtc agatgcgccg gaacggccag
     2281 attccaacag aagtcaacat ctcctacaaa gactacagta acgagatcgt tatcaatacc
     2341 gaccgcggac gtgcccgccg cccgctgatt gttgtggaaa acggtgtacc tgccgtcact
     2401 gatgaggata ttgaaaaggt ccggtctggt gagtatgagt tcatgacgct tgtccagcag
     2461 ggcaagatcg agttcattga tgcagaggaa gaggatgatc tctacattgc cgtctatgaa
     2521 gaggacttaa ccccggagca cactcatctg gaaattgacc cgtcgcttat cctcggtatt
     2581 ggtgctgcac acgtcccctt cccagagcac aacgcatctc cgcgtgtcac catgggagca
     2641 ggaatgatta agcaggccct cggatttgcc cagtcaaata tgaaactgcg gccggatacc
     2701 cgtggtcaca tgctccacta tgcccaggtc ccgatggttc acacgcaggc agcagaactg
     2761 attggttccg acaaccgccc gcagggtcag aacttctgtg ttgcaatcat ctcgtatgaa
     2821 ggatacaaca ttgaagatgc actcattttc aacaaagcct ctgtggagcg tggtcttgga
     2881 cgttcccact acttcagaac ctatgaa
//
LOCUS       DALUAJ010000197         2509 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_38549,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000197 DALUAJ010000000
VERSION     DALUAJ010000197.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38549"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="O0X37_04170"
     CDS             complement(<1..666)
                     /locus_tag="O0X37_04170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HJK62149.1"
                     /translation="MIDEIRYARSGDAKIAYRITGSGMPLVIVSGLGDSMKDWKESII
                     RTLAERFCVILPDNRGLGLTGMGSIPPQKMTIIQYAEDIHAVVEAEGVSEIFLLGHSM
                     GGMIAQEFAVLYPEMVKKLMLFATDYGPESSYRAHLMNRCVLPLQALCVVAPWHTKGF
                     RAGACAIASWPGSADRLGDICCRTFLLFGDADFLMHPDVAHEMERMIPDAELKMIPGG
                     THRM"
     gene            complement(741..1013)
                     /locus_tag="O0X37_04175"
     CDS             complement(741..1013)
                     /locus_tag="O0X37_04175"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit L"
                     /protein_id="HJK62150.1"
                     /translation="MKLKIIELEKGKIRLSLEGEGHTFMNALTEEILIDPEVDVAKYV
                     IEFQFSDPELFITMKEGSKKDPVTVIREACDRISTRCDDLLGCLKN"
     gene            1277..1361
                     /locus_tag="O0X37_04180"
     tRNA            1277..1361
                     /locus_tag="O0X37_04180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1311..1313,aa:Ser,seq:gct)
     gene            1454..1909
                     /locus_tag="O0X37_04185"
     CDS             1454..1909
                     /locus_tag="O0X37_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HJK62151.1"
                     /translation="MVEESELKYFVRIINNDLDGTRPVQLALTGIKGIGLHAALVISR
                     RAGVDTHATMGLLADEDVARLEEQVLAYPTSVPAWMVNRPVDVYTGVPKHLYGSDLAL
                     AKEDDINLMKKMRCYRGIRHENGLKVRGQCTKSTGRFGKIVGVSKRRNA"
     gene            1926..2471
                     /locus_tag="O0X37_04190"
     CDS             1926..2471
                     /locus_tag="O0X37_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HJK62152.1"
                     /translation="MGYPGKNTKQYSSPKRRFEKSRIESERVLAITYGLRNKREIWRA
                     TDILRKHRGGAREVLAMMSAVGETPKTVARRDELINTLQRYGMIGANAAMDDILSLKV
                     EDVLERRLQTIVYRKGLARSPKQARQLITHGHIAINGQRVSVPSYMVSVSEEAGIAYY
                     ATSSLADDANGERQRIMNVRA"
BASE COUNT          637 a          713 c          601 g          558 t
ORIGIN      
        1 catccgatgt gtcccgccgg gaatcatctt cagttctgcg tcagggatca ttctctccat
       61 ctcatgcgcc acatcaggat gcatcagaaa atctgcatcc ccaaacagca aaaatgtccg
      121 gcagcaaatg tcaccaagcc ggtctgccga tcccggccac gacgcaatcg cacatgcacc
      181 tgcccggaac cctttcgtgt gccacggtgc aaccacacac aatgcctgga gcgggagcac
      241 acaacggttc atcagatgtg cacggtatga cgactccggt ccgtaatccg ttgcaaacag
      301 catgagtttt tttaccattt ccggatacaa aaccgcaaac tcctgggcaa tcatcccgcc
      361 catggaatgc ccgagcagga aaatctccga gaccccttct gcttctacca ccgcgtgaat
      421 atcctccgcg tactgaataa ttgtcatctt ctggggagga atcgatccca tcccggtcag
      481 accaagacca cggttgtccg gcagaattac acaaaacctc tctgccagtg tccggatgat
      541 cgactccttc cagtccttca tcgagtcgcc aagaccactg acgatcacaa gaggcattcc
      601 ggaaccggta atccggtatg caatttttgc atcccctgat ctcgcatacc gtatctcatc
      661 aatcatggtt caaaaaagta ttattggttt tgtcccccat aaacatgcca tcgctgggga
      721 accggtcata tcctgtttgt tcagtttttc agacatccaa gcagatcatc acatctcgtc
      781 gagatgcggt cacatgcctc acgaataacc gtaaccggat ccttctttga cccttccttc
      841 atcgtaataa agagctccgg atcggagaac tggaactcaa tcacatactt tgccacatca
      901 acctcggggt ctataagaat ctcctcggtc agtgcattca taaatgtgtg tccttcccct
      961 tcaaggctca gccgtatctt gcctttctca agctcgatga tcttcagctt catgcggatt
     1021 acctatttgg cggatgagac tatatagatg acgcaggata cgtcatcaaa cgatccgatc
     1081 ttggataagt attatatcat cccatgccct atctattaaa gcactcaatt tagtggagcg
     1141 gtgtgtcggg caacacccga acgttcacac gaggatcctt cccgtgcgaa gtggcaccgc
     1201 gagtgtcgaa agcatatcaa accgtgagcg gtttgtatgc tgcctgcaga aatgcaggaa
     1261 agatgcggag ttctctgtag acgtagccaa gcctggtatg gcgcaggttt gctaaacctg
     1321 tgtccccctg ggactcgagg gttcaaatcc ctccgtctgc gcttattcca gggatgcaac
     1381 atccctgaaa cgttcaggag tacgaagcgc tcataacgta tgagacttat ctctctactg
     1441 agaggcgact ataatggttg aagagtcaga actgaaatat tttgtgcgga tcattaacaa
     1501 tgatctggac ggtacccgtc cggtacagct tgcactgacc ggaattaagg gcatcggcct
     1561 ccacgccgca ctcgtcatct cccgccgtgc aggagtcgac acccacgcaa cgatgggtct
     1621 cctcgcagac gaagatgtcg cgcgccttga ggagcaggta cttgcatacc cgacctcagt
     1681 cccggcatgg atggtcaacc gcccggtaga tgtgtacacc ggagttccga aacacctcta
     1741 cggcagcgac cttgcactcg caaaagaaga cgacatcaac ctcatgaaga agatgcgttg
     1801 ctatcgcggt attcgtcatg agaacggcct gaaggtacgt ggtcagtgca ccaaatccac
     1861 cggacggttc ggaaagattg tcggtgtttc caagaggcgg aacgcataat tcacggtgat
     1921 gaagaatggg atatccagga aagaacacca aacagtactc ctccccgaaa cgccgctttg
     1981 aaaagtcgcg tatcgagagc gagcgtgtac tcgctatcac ctacggtctt cgtaacaagc
     2041 gtgagatctg gagagcaacc gacatccttc gcaagcaccg cggaggagcc cgtgaagtgc
     2101 tcgcaatgat gtccgctgtt ggcgaaaccc cgaaaaccgt cgcacgccgc gacgaactga
     2161 tcaacaccct tcagcgctac ggcatgattg gtgcaaacgc cgcaatggat gatattctct
     2221 ccttaaaggt tgaggacgtc cttgagcgcc gtctgcagac catcgtctac cgcaagggtc
     2281 ttgcacgcag ccccaagcag gcacgccagt taatcaccca cggtcacatt gctatcaacg
     2341 gtcagcgtgt gtccgtcccg agctacatgg tttccgtctc cgaagaggca ggcattgcat
     2401 actatgcaac ctccagcctt gcagacgacg caaacggtga acgccagcgt attatgaatg
     2461 tgagggcgtg aagacaatgt cagaagcaag cgaaaagtgg ggcattgca
//
LOCUS       DALUAJ010000198         1933 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_39035,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000198 DALUAJ010000000
VERSION     DALUAJ010000198.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39035"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="O0X37_04195"
     CDS             <1..1063
                     /locus_tag="O0X37_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication factor C large subunit"
                     /protein_id="HJK62153.1"
                     /translation="IANDAYGVSDSIRKLCDPIPFRAITTSTLEKRMREICSAEQITC
                     GSDALSAIAESAAGDLRSAVNMLFASATGKSVIASDDINTSQKDERASIFDLVAGVYA
                     GYPDEKIQKLSRECEEKPDTVMQWIEESAAAVTNQNQRTGTYTALSRADIYLGRTMMR
                     QYYTLWRYATSLMTSGVAKEFTSAGFRPRIMPPSRWKRMGTAKKQKSVRRTLAAKLGE
                     SYSIPDSQIQKVYLDLLSRFATDAPLAFCERHDLDADQLTILLHDKTEATSVFKEAQK
                     IAKEKETKMKKISAAKRAAARKAEEERTAGLEAFHQETTSASTTPGVVSAAEPIKENV
                     DPPQEEKKKTPTQATLDFF"
     gene            1191..1571
                     /locus_tag="O0X37_04200"
     CDS             1191..1571
                     /locus_tag="O0X37_04200"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HJK62154.1"
                     /translation="MKYIVDDKELTASQFIEFVNNVWPGEYDREKTRIALTKTLNITA
                     YDNNMLVGCLRILSDGYYFGTITEILVLPEYQKQGIGSKLLQLAKDSTPTMLYFGSQP
                     RVEKFYEKNGCLRGLTSYTIETKR"
     gene            complement(1643..>1933)
                     /locus_tag="O0X37_04205"
     CDS             complement(1643..>1933)
                     /locus_tag="O0X37_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HJK62155.1"
                     /translation="DHIGEYDAIIPEWENTDIEPLHAVYRVSAVRQYVEEHESLSLRD
                     MVNSLHTLRVSPEEFRQFDPNLETFQNINTLDELQALGPDAAYLEKHPEKQG"
BASE COUNT          590 a          475 c          458 g          410 t
ORIGIN      
        1 catcgcaaac gatgcgtacg gggtctcaga ctccatccga aagctgtgtg acccgatccc
       61 gttccgcgct ataaccacat caacgcttga aaaacgtatg cgggagatct gttccgcaga
      121 acagataacc tgcggcagtg atgccctgag tgccattgca gaaagtgccg caggagatct
      181 gcggtccgcg gtcaacatgc tcttcgcttc agcaaccgga aaatccgtaa ttgcttcaga
      241 tgatatcaac acctcgcaga aggatgaacg ggcaagtatc tttgatcttg tcgcaggcgt
      301 gtatgcaggg tacccggatg agaaaatcca gaaactctcg cgcgaatgtg aggagaaacc
      361 ggataccgtc atgcagtgga tcgaggagtc tgcagcagct gttacaaacc aaaaccagcg
      421 gacaggcacg tacactgccc tctcccgagc cgacatctat cttggcagaa ccatgatgcg
      481 gcagtactac actctttggc ggtatgctac ctccctgatg acctcaggcg ttgctaaaga
      541 gttcacctca gcaggattcc gtccccgcat aatgccgccg tcgcggtgga aacggatggg
      601 aaccgcaaaa aaacagaaaa gtgtcaggag aacgcttgct gcaaaacttg gagaaagtta
      661 cagtatcccc gactctcaga tacagaaagt ctatcttgat ctcctgtccc gctttgctac
      721 ggatgcaccg cttgctttct gcgagcgcca tgacctggac gcagatcagc ttaccattct
      781 cctgcacgat aaaactgagg cgacctcagt ctttaaagaa gcccaaaaga ttgccaaaga
      841 aaaagaaacc aagatgaaaa agatctcagc agcaaaacgt gctgccgcac gaaaagccga
      901 ggaggaacgt acagcaggtc ttgaagcatt tcatcaggaa acaacgtccg catccacgac
      961 acctggtgtg gtgtctgccg cagaaccgat aaaagagaat gtcgaccctc cgcaggaaga
     1021 aaagaaaaaa actcctacgc aggcgacact ggatttcttt taatcatctg ctgtgaagta
     1081 catcccatca tctaatgtgg aaaaacagtg cggtaaaacc aggtacatga gaacacagga
     1141 ggggaaacct gcaacaaaag ggttgttgaa tttggttgag atgactaaca atgaaatata
     1201 tcgtggatga caaggagctt acggcatctc aatttataga atttgtcaac aacgtgtggc
     1261 cgggcgagta tgacagggaa aagacacgga tagcattgac aaaaacattg aacataactg
     1321 cttacgataa caatatgctg gtgggctgcc tgcgtattct ctcagacggt tattattttg
     1381 gaacaattac agagatactt gttcttccag agtaccaaaa gcagggtatc ggtagtaaac
     1441 tactgcaact cgccaaggac agtacaccaa caatgctgta ttttggatca cagcccaggg
     1501 tggaaaagtt ctacgaaaag aacgggtgcc tgagaggatt gacatcctac acaatagaaa
     1561 caaaaagata ggcaaattaa atgcacgcag tcccctgtga ccagaccgaa ctccgaacaa
     1621 aaagattcgg aaatgtaaaa aattatccct gtttttcagg atgtttctca agatatgcag
     1681 catcaggacc aagagcctgc agttcatcca gcgtattgat gttctgaaag gtctcaagat
     1741 ttggatcaaa ctgccggaac tcttcaggac tcacccgaag cgtatgcaga gagttcacca
     1801 tatcccgaag tgacagggac tcatgctcct ctacatactg ccgcactgca gaaacacggt
     1861 acaccgcatg cagcggttca atatccgtgt tctcccactc gggaataatt gcatcatact
     1921 caccgatgtg atc
//
LOCUS       DALUAJ010000199         1584 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_39102,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000199 DALUAJ010000000
VERSION     DALUAJ010000199.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39102"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="O0X37_04210"
     CDS             complement(<1..882)
                     /locus_tag="O0X37_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HJK62156.1"
                     /translation="MMEDITSTVEFQMSLLLFVALGGYLLATRIHQSAVIGEILVGLI
                     VGPSVLGLITYTDFVQSLAGLGAIILLFVIGFEFELSDITNWRYGVIALIGVIIPWIG
                     GYATSLFFNMDFYTAIFIGTALTATSIAITANVLREMGKLHQPFAKAIIGAAVIDDIL
                     SLIVLSICKDLGSTGELVPVAVLLTVIKSIGFVVIAAVVGIKVIPPLISKMDTTKLAR
                     QFPEFVFIFAMMVAFFYAMMAESVGVSAIVGAFLAGVCVNRVSLKHSLDIKLGAEYLY
                     IIFAAIFFVSLGIIVDLQ"
     gene            complement(936..1382)
                     /locus_tag="O0X37_04215"
     CDS             complement(936..1382)
                     /locus_tag="O0X37_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HJK62157.1"
                     /translation="MVQIPAFETVAQAHGHTCPGIALGYKIAVVAARWVGDDDDVRIV
                     AHTTRCPLDALKVTFDVRNHPERMVVEDTNTVSFVITKPNGKKLFIDELPGTHLTSDE
                     LTEIKKKVEAGTATADDLRRMNEIKNELFRIMQAIPDEKLFAVREE"
     gene            complement(1415..>1584)
                     /locus_tag="O0X37_04220"
     CDS             complement(1415..>1584)
                     /locus_tag="O0X37_04220"
                     /inference="COORDINATES: protein motif:HMM:NF003085.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor 1A"
                     /protein_id="HJK62158.1"
                     /translation="VTRLGRIKGKIKKRVWIREGDILIVIPWEFQDDKCDIIYRYTTP
                     QVDWLRAHKYL"
BASE COUNT          428 a          372 c          383 g          401 t
ORIGIN      
        1 ctgaagatcc acgataatgc ccagcgacac aaagaagatt gccgcaaaga tgatgtacag
       61 atactccgca ccgagtttga tgtcaagact gtgtttcagg ctcacacggt tgacacagac
      121 accggcaagg aatgccccga caattgcaga gacacctact gactctgcca tcattgcata
      181 gaagaacgca accatcattg caaagatgaa cacgaactcc gggaactgcc gggcgagttt
      241 ggttgtatcc atcttggaga taagcggcgg aattactttg attccgacga ctgctgcaat
      301 caccacaaat ccgatcgatt taattaccgt cagaagaact gccaccggca caagttcacc
      361 ggttgaacca agatctttac agatggacag tacgatcaga ctgagaatat cgtcgatcac
      421 cgcagcaccg ataatagcct ttgcaaacgg ttggtgcagt tttcccatct cccgcaaaac
      481 gttcgccgta atcgcaatac ttgttgcagt gagtgctgtt ccaatgaaga ttgcggtgta
      541 gaaatccatg ttgaaaaaca gggatgttgc atatcctcct atccagggga tgattactcc
      601 tatgagggct atcaccccgt atctccagtt ggttatatca ctgagttcaa actcaaagcc
      661 aatcacaaaa agcagaataa tggcgccaag acctgccaga ctctggacga agtcggtata
      721 ggtaataaga ccgagtacac tcggaccaac aatgagaccc accagaattt cgccgataac
      781 cgcagactga tgaatgcgtg ttgccaggag atatccaccg agggccacga agagcagcaa
      841 actcatctgg aactcgacgg tggatgtgat atcctccatc ataattcaat ttcacatggg
      901 ggttgtgacg taaaataaat atcgttagga cgaagttatt cttcgcgaac ggcgaataat
      961 ttctcgtccg ggattgcctg catgatgcgg aaaagttcgt ttttgatctc gttcatgcgg
     1021 cgaagatcgt ctgcggttgc tgtacctgcc tctacctttt tcttgatctc ggtgagttca
     1081 tcgctagtaa ggtgtgtccc aggcagttca tcgatgaaga gttttttgcc gtttggtttt
     1141 gtaattacaa aactgacggt gttggtatcc tcgacaacca tgcgttcagg atggtttctt
     1201 acatcaaagg taactttgag agcgtccagt gggcaccggg tggtgtgggc gacaatccgt
     1261 acatcgtcgt cgtcaccaac ccatcttgct gcaacgacgg caattttgta accaagagca
     1321 ataccgggac aggtatgtcc gtgagcttgt gctactgttt caaatgctgg aatctgtacc
     1381 atgattgttc tgagaaaaaa tggtgatgat gtgtttagag gtatttgtgt gcgcggagcc
     1441 agtccacttg cggggtagtg tagcggtaga taatatcaca tttgtcgtcc tggaactccc
     1501 agggaataac aatgagaatg tctccttcgc ggatccagac ccgtttctta atcttacctt
     1561 taatccgacc gagacgtgtg acac
//
LOCUS       DALUAJ010000200         3244 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_39366,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000200 DALUAJ010000000
VERSION     DALUAJ010000200.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3244)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3244)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3244
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39366"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            126..380
                     /locus_tag="O0X37_04225"
     CDS             126..380
                     /locus_tag="O0X37_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62159.1"
                     /translation="MTEPKTIRERYNDTQSRILGYLKAGVAKGSRFFKAKYIAKDLGL
                     SSKEVGTNLAILSEICDELDIQRWSYSNSTTWMVTSKTIA"
     gene            532..816
                     /locus_tag="O0X37_04230"
     CDS             532..816
                     /locus_tag="O0X37_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62160.1"
                     /translation="MKHLLMVSEKELQVQVPQYNSTQQRILNYLKAGVASGTRFFKAK
                     YIAKELGISSRAVGTNLALLAEKCLEFEIVQWGYSTSTTWMVKLRTLVNS"
     gene            977..1156
                     /locus_tag="O0X37_04235"
     CDS             977..1156
                     /locus_tag="O0X37_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62161.1"
                     /translation="MGKKIAEIVETIEFVADIEGAKDCLMSQQGELWFNIDVPKGHGL
                     KNGDKIRVIVERADE"
     gene            1159..1566
                     /locus_tag="O0X37_04240"
     CDS             1159..1566
                     /locus_tag="O0X37_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62162.1"
                     /translation="MNKKAGQTVTIKRMVLVLIGIIAVLTLAIVLTVGATSIDADDPV
                     YINGKLVTAFEYNGEPRDVWVQCTISRVDDLFAEEQVGGLMTNAPTFTSGRNICEFPI
                     ELEPGTYKVRIYVRERNEGAVRIAAFIKNFEIV"
     gene            1563..3170
                     /gene="ade"
                     /locus_tag="O0X37_04245"
     CDS             1563..3170
                     /gene="ade"
                     /locus_tag="O0X37_04245"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HJK62163.1"
                     /translation="MIHVFDNAFLFNPCTGEWLLTSFSVENGIVAAIGANHSLTGDQI
                     TDLGGSRVIPGLIDTHLHIESTFLVPQEFGRVALAHGVTTAIADPHEIANVAGTAGID
                     FMLQDAQCSPNDIFFMLPSCVPATAEDVGGAVLTAADLAKYSENPRVLGLGEMMNFPG
                     VLADDPEVLAKLSLFSHVDGHAPGLVGTDLCRYAAHGIKTDHECTSAEEAREKLRCGM
                     YILLREGEAARDVEALSSIVDASTVSRCCFCTDDRHVDTLVREGSIDHCIRIAIAAGM
                     PENLALRMATLSAAECFGLTDRGVIAPGRVADFCILADTEIFTIQKVYKNGIPADEIP
                     ISAKQTPYQVPPFSCRFPTQTDLSLPENGTARVIGIIPGELVTENIPARPDADGVQKL
                     LSIDRYHAGGFAIGLVKGFGMQTGAVASSIAHDAHNIIATGKTDEEILTAIRAVADAG
                     GGLAVVTGKEITLLSLPIGGLMTYEPADSLLRGLDTLSAHLKRTGACRQTFGYLSFLS
                     LTVIPHLKLTPRGLFDVDAFETVPLFH"
BASE COUNT          825 a          749 c          785 g          885 t
ORIGIN      
        1 acctttggtt atgtggattc tttttcataa cagcggtcgt tcccgctgaa ttcattatca
       61 cacctgtatg tcaagcacta tctttataat gtaaaacatc ctatacttac aagaggtaag
      121 tcattatgac cgaacctaaa acaattcgtg aaagatataa cgacacccag tctcggatcc
      181 ttggctatct gaaggcagga gtggcaaaag gaagccgttt cttcaaggca aagtacattg
      241 ccaaagatct tggtctttcc tccaaagagg ttggaacaaa ccttgccatc ctttccgaga
      301 tctgtgatga actggacatc cagcgctgga gttactccaa cagcaccacc tggatggtaa
      361 cgagcaaaac aatagcataa atacttcacc cctctttctt ttttgctccc ttacgcattg
      421 taaccaatat caacaacata ttctctgcgc gattgtctat ctctgcggta ttttgccttt
      481 gttccaactg tatcatttta ggagtcaaag aggaagtata tatattataa aatgaaacat
      541 ttacttatgg taagtgaaaa ggagcttcaa gttcaggtcc cgcagtacaa ctcaactcag
      601 cagcggattc tcaattatct gaaagcaggt gttgcatcag gcacccggtt cttcaaagca
      661 aaatacattg caaaagagct tggtatctct tctcgggcag tcggtacaaa tcttgctctc
      721 cttgctgaaa aatgtctgga gtttgagatt gtgcagtggg gttactcgac cagtaccacc
      781 tggatggtaa aactcagaac gctcgtgaac agttaatccg gaaaaatacg agctcctttg
      841 tgcgacacat ttagaatact cttttgtttt tgatacaaca tcccaacccg cccaattttt
      901 ggaactggat gtacgctttg cagtaaatcc gatgcgaacg ttactttaat aaataatccc
      961 tgcctcattt ttatctatgg gaaaaaagat tgctgaaatc gtcgaaacca tagagtttgt
     1021 tgcagatatt gaaggggcaa aagactgtct gatgtcccag cagggcgaac tctggtttaa
     1081 cattgatgtc cccaaaggtc acggcctgaa aaacggcgat aaaatccgtg taatcgtaga
     1141 gcgtgctgat gaataatgat gaataaaaaa gctggtcaga cggttaccat aaaacggatg
     1201 gtactggtcc tgataggaat cattgcagtt cttaccttgg caattgtctt gacggtaggg
     1261 gctacctcga ttgatgcaga cgatcctgtg tatatcaacg gaaaacttgt tactgcattc
     1321 gagtacaacg gagagccgcg tgatgtgtgg gtgcagtgta cgatctcccg agtggacgat
     1381 ctctttgccg aggaacaggt aggtggtctt atgacaaatg cgccaacatt tacctccggc
     1441 agaaatatct gtgagttccc gatagaactg gaaccgggaa cctataaagt acggatctat
     1501 gtcagggagc ggaatgaagg agctgtacgg attgcggcgt tcattaaaaa ttttgagata
     1561 gtatgatcca tgtttttgac aatgcctttc tcttcaatcc gtgtaccgga gaatggctac
     1621 taacttcttt ttccgtggaa aatggtattg ttgctgcaat tggtgcaaat cacagtctga
     1681 ctggggatca gatcaccgat cttgggggat cacgtgtcat ccccggactg atcgataccc
     1741 acctgcatat tgaaagtaca tttctggtac cgcaggagtt tggcagggtt gctcttgcgc
     1801 acggtgttac cactgcaatc gctgatcccc atgaaattgc caatgtggcc gggactgctg
     1861 gaatcgattt catgcttcag gatgcacagt gctcaccaaa cgatattttc tttatgcttc
     1921 cctcgtgcgt acctgcaact gccgaggatg ttggaggagc tgttcttacc gcggctgatc
     1981 ttgcaaaata ttctgagaat cctcgggtac tgggccttgg ggaaatgatg aacttcccgg
     2041 gagttctcgc agatgatcct gaggttcttg caaaactttc tctgttttcc catgtggacg
     2101 gacatgctcc aggtctggtt ggaactgatc tctgccggta tgcggcccac gggatcaaga
     2161 cggatcacga atgcacctcg gcagaggagg ccagggaaaa actccggtgt ggtatgtata
     2221 ttctgttgcg ggagggtgag gccgcacggg atgttgaagc cctttcttcg attgttgatg
     2281 caagtaccgt ctcacgctgc tgtttctgta cagacgatcg gcatgttgac acgcttgtcc
     2341 gcgaaggatc catcgaccac tgtattcgta ttgcaatcgc cgcaggcatg ccggagaatc
     2401 tcgcactccg gatggcaacc ctgtctgctg cggagtgttt tggtctcact gaccggggtg
     2461 tgattgcacc ggggcgggtt gctgacttct gtattcttgc agatacagag atctttacaa
     2521 tacaaaaggt ctacaaaaac ggtatccctg ccgatgagat ccctatttct gccaagcaga
     2581 caccatatca ggttcctccg ttttcatgca ggttcccaac ccaaacagat ctttctctgc
     2641 cggagaacgg taccgcccgg gtcatcggta ttattcccgg agagctggta actgagaaca
     2701 ttcctgccag acctgatgca gacggagtgc agaaactcct ctctattgac cgctatcatg
     2761 ctgggggttt tgccattggt cttgtgaagg gttttgggat gcagacaggg gctgttgcct
     2821 cctcaattgc ccacgatgcc cacaatatca ttgcaaccgg caaaaccgat gaagagattt
     2881 tgactgcaat tcgtgcagtc gctgatgcag gaggaggtct tgcggttgtc accgggaaag
     2941 agattacact gctttctctt cccatcgggg gtctcatgac ctatgagccg gctgactccc
     3001 tgctgagagg gctggataca ttatccgcac atctcaagcg aaccggtgca tgcagacaga
     3061 cctttggtta tctctccttc ctgtctctga cggtaatccc gcatctgaag ctaacgccgc
     3121 ggggtctttt tgatgtggat gcgtttgaaa ccgtccctct ctttcactga tttttcttca
     3181 acaggaaagg atattgagga agaacagcaa tactgattag aacgatgaag ctacagcgga
     3241 atat
//
LOCUS       DALUAJ010000201         5913 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_39420,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000201 DALUAJ010000000
VERSION     DALUAJ010000201.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39420"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="O0X37_04250"
     CDS             <1..728
                     /locus_tag="O0X37_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62164.1"
                     /translation="IYLPKHAYIHVSMADAQEGTPSYGQPVCSKVIPPQIDEETARSI
                     VRKWFGSDHFPFVPGEKMRFGKAVLVYYPFWRYRREDGGEEKTIYRPACGTLLTGLQN
                     IKYYHDTEAVDTPEDVTVLPVTVDSSVYLPELHGIARGEELIGVPLWLISYKVKHNIY
                     MIEVEAHSGLVLPEWHPIKEPVNWKKTAMTAFIPMFLLSLVAIYFNPWIFVIVVILLF
                     IFLYQSEMLGLITMKEREEPHGP"
     gene            718..2214
                     /locus_tag="O0X37_04255"
     CDS             718..2214
                     /locus_tag="O0X37_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62165.1"
                     /translation="MGLERSVEDRVGRVMYRSKVTKGVADRTIRDWFTKGIKAPDLPS
                     KAVMTELYMIYIPFWRFVAQGKAVACGYSEYNEKTGNTIRNIYKELIDDEFVWTEVAC
                     DTGKYGLTKLWLEPGGEVPYTPGAVVSMEPGGSAVEASRRGREAVRQMIDELAAKRID
                     TLTLEKSFLLPKVFELVYAPIWVAHYEYQGGDYTAVLDAVRGEILGGTAPINLTARTR
                     MMILSLAAGGIMIGSSLAMLLHAGAHPLSELFQVILLLLGVAMTMAAYPAFKEGRTFV
                     STGTMKHISSLRPAVRIPKKLTDQEILKRDSTILLCPVCGEEVEQPWGEVISVCKKCH
                     RLLNVTSNEVNVVPYDVAKPDMLAQAALESEPEYIPFWMFEVENKVTDLLRTGDTETG
                     LPEISGRRNYYICAADIPRYLAERWEIDLTIRNPEFSELPLNEHVEMKPAILNQNTAK
                     ELSEFLFLRYEEEKPGILQVLRYDFAVKSSRIVYVPYYVEDKTYIPGV"
     gene            complement(2265..3092)
                     /gene="nadC"
                     /locus_tag="O0X37_04260"
     CDS             complement(2265..3092)
                     /gene="nadC"
                     /locus_tag="O0X37_04260"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HJK62166.1"
                     /translation="MLPTDQLLSFFAEDVPAGDITTLALVPDSTVSARIEAREDMVLA
                     GVEECVWLFEHFGVSVTVISEDGRRVSAGARILSLAGSVQVILAVERTALNLLGRMSG
                     IATATATAQIRASVVNDHVRVAGTRKTAPGLRYFDKKAIRLGGGDPHRDSLSDAFLIK
                     DTHRALLPVAEAVRRAKEYSAYHLVECEVESIDDAVAAAEAGVDILMFDNMTPDAVRE
                     AIAVLAGMGLRDRLVLEVSGGITPETIHLWAGVDVDRISMGSLTHSVRCADVSLEIE"
     gene            complement(3092..3994)
                     /gene="nadA"
                     /locus_tag="O0X37_04265"
     CDS             complement(3092..3994)
                     /gene="nadA"
                     /locus_tag="O0X37_04265"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HJK62167.1"
                     /translation="MNYEQQIKKLAAEKNAIILAHNYQPAEIQDVADICGDSLELARL
                     AKEAKESTLVHCRVYFMAETAKILSPEKTVLIPRPEAGCPLADQLTPEMVRHAKAEHP
                     GSPFVVYVNSNAATKAECDITCTSANAAVIAASLPEKRILFGPDANLAGWVQRQLPDK
                     EIIPVPANGGCPTHHNFTVRDVEEARRMYPNATVICHPECSSEVQQACDLVGSTGYMV
                     RNCGTADEWVILTEKGILRPLEKRYPDTVFHVMETAVCPSMKVVDLSDLYVTLRDGVY
                     PVVVADEIANRARSAIERMIEASK"
     gene            complement(3991..4749)
                     /gene="nadX"
                     /locus_tag="O0X37_04270"
     CDS             complement(3991..4749)
                     /gene="nadX"
                     /locus_tag="O0X37_04270"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HJK62168.1"
                     /translation="MITVGLLGCGNIGRVIAAGQENFKITAVYDVVPARVEEFAREFN
                     AKPFSDFSAFLAEPTDIIVEAASVVAATEHAQEVLESGKDIVIMSVGALADKEFRAHL
                     VETARSLKRKIHIPSGAVMGLDNIRIGQISRVDKFVLRTTKNPKSLSREATERTQLFS
                     GKAHDCVRQYPKNTNVAESLSIACGRDVDVELWMDPEEDRNMHEIFFEGEFGEAYIRI
                     RNLPSPDNPATSYLAALSILTLLKNLDNPLVIGA"
     gene            complement(4868..5083)
                     /locus_tag="O0X37_04275"
     CDS             complement(4868..5083)
                     /locus_tag="O0X37_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HJK62169.1"
                     /translation="MPIILRLDRVMADRKMSLNELSEKVGVTNVNLSKIKTGKISAIR
                     FSTLEAICRVLNCQPGDILEYQPEEKS"
     gene            complement(5091..5681)
                     /locus_tag="O0X37_04280"
     CDS             complement(5091..5681)
                     /locus_tag="O0X37_04280"
                     /inference="COORDINATES: protein motif:HMM:NF022636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HJK62170.1"
                     /translation="MNEVEIQSKIKQNAKNVGIVLTIGKILVVFVIVLLFLTAALTAC
                     IYTVGGPAEFSHWIVSEDVLSSFGVGTSVPAGMDPTTAFIQASAIFLVSATVYLLTFA
                     GMIWAAARIFAEIRESGVPFTQANARSLKHVAICMALLCVVPVIIDSIGWYITGISVT
                     VGYLSIEGLIVAAVLYCLAHIFEYGTLLQKQADETL"
     gene            complement(5695..>5913)
                     /locus_tag="O0X37_04285"
     CDS             complement(5695..>5913)
                     /locus_tag="O0X37_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62171.1"
                     /translation="SYYLSLATVTAAAGLAAVLIVLGWSVMFGYCSLTVESFHLLSLL
                     VLGTAGSLVLLILLYLHFVVHHASSHPS"
BASE COUNT         1536 a         1502 c         1511 g         1364 t
ORIGIN      
        1 acatctatct tccaaaacac gcatatattc acgtatctat ggcagatgca caggagggga
       61 cgccgtcata tgggcagcct gtctgttcaa aggtaattcc cccgcagatt gatgaggaga
      121 ctgctcgcag tattgtccgc aagtggtttg gctcggatca ttttccgttt gtgccgggcg
      181 agaaaatgcg gttcggaaag gcggtgctgg tgtattaccc gttctggcgc tatcggcgtg
      241 aagacggcgg tgaagagaaa acgatctacc gtcccgcctg tggtacgctt cttaccggcc
      301 tgcaaaatat aaaatactat catgacaccg aggcagttga tacaccggag gatgtgactg
      361 tgctgccggt aaccgtggac tcatccgtgt atctgccaga acttcacggg attgcacgcg
      421 gcgaggagct gatcggggtg ccgctgtggt tgatcagtta caaggtgaag cataacatct
      481 acatgattga ggttgaggca cactctggtc tggttctgcc ggagtggcat ccgatcaaag
      541 agccggtgaa ctggaagaag acagcgatga cagcatttat tccaatgttt ctgctaagtc
      601 tcgttgcgat ttacttcaat ccgtggattt ttgtgattgt ggtgatacta ctgtttatct
      661 tcctttatca gagtgagatg cttggcctga ttacgatgaa agagagggag gagccgcatg
      721 ggccttgagc gttcagttga ggatcgcgtt ggacgggtaa tgtaccggtc gaaggtgacg
      781 aaaggcgttg ccgaccggac gatccgcgac tggtttacta agggcatcaa agcaccggat
      841 cttccaagca aggctgtgat gacggaactg tatatgatct acattccgtt ctggagattt
      901 gttgcccaag ggaaagcagt cgcctgcggt tattcggagt ataatgagaa gacaggcaat
      961 acgatacgca acatctacaa agaactgatc gatgacgagt ttgtgtggac ggaggttgcc
     1021 tgtgatacag ggaagtatgg ccttacaaag ctctggctgg aaccgggagg ggaagtcccc
     1081 tatacaccgg gagcagtagt ctcgatggag ccagggggat cagcagttga ggcaagcagg
     1141 cggggacggg aagcagtgcg acagatgatc gatgaactgg cagcaaagcg gattgatacg
     1201 ctgacactgg aaaagtcgtt ccttttgcca aaggtctttg aactggtata tgcacccatc
     1261 tgggttgccc attacgagta tcagggcggg gattatacgg cggttcttga tgcggtgcgc
     1321 ggagagatcc ttggcggcac agcaccgatc aatctgactg caagaacacg gatgatgatt
     1381 ctgtcgcttg cagcgggcgg tattatgatc ggatcatcac ttgcaatgct gctgcatgca
     1441 ggagcacatc cactctcgga actgtttcag gtaattttac tgttgctcgg tgttgcgatg
     1501 acaatggcgg cgtatccggc ctttaaggaa ggaaggacgt ttgtgagtac cggtacaatg
     1561 aagcatattt ccagtctgcg gcctgcggtg cggataccaa agaagctgac agatcaggag
     1621 attctgaaac gggactcaac gattcttctc tgtcctgtat gtggcgaaga ggttgagcag
     1681 ccgtggggtg aagtaatttc agtctgtaaa aagtgtcaca ggttactgaa tgtgacaagc
     1741 aacgaggtga acgtggtacc gtatgatgtt gcaaagccgg acatgcttgc acaggcagcc
     1801 ctggaaagtg aacctgagta tattccattc tggatgtttg aggtggagaa taaggtgacg
     1861 gatctgctgc ggaccggtga tacagagaca gggctgccgg aaatttccgg aagacggaat
     1921 tactatatct gtgcggcgga cattccccgg taccttgcgg agcggtggga gattgatctg
     1981 acgatcagga acccggagtt ttctgagcta ccgctgaatg agcatgtgga gatgaagcca
     2041 gcaattctga atcagaatac ggcgaaggag ctgtcggagt ttttattcct ccgttacgag
     2101 gaggaaaaac ctggaatatt acaggtgctt cggtatgatt ttgcggtaaa gagttcgcgg
     2161 attgtgtacg tgccgtacta tgttgaggac aaaacgtaca ttccgggagt ctgaatcttt
     2221 ccttttttga tgtctgggat atccagctat accacaaacc agatctactc aatctccaaa
     2281 ctcacgtcgg cacaccgtac cgaatgcgtc agagacccca tgctgatccg atccacatca
     2341 actcccgccc acagatgaat tgtctccggc gtaatccccc cggacacctc aagcaccagc
     2401 cggtcacgga gacccattcc tgcaaggacc gcaatcgcct cccgtaccgc atcaggcgtc
     2461 atattatcaa acatcagaat atcaactccg gcctccgctg ccgcaaccgc atcgtcaata
     2521 ctctccacct cacactccac cagatgatat gctgagtact cctttgcccg ccggacagcc
     2581 tcagccaccg gcaaaagggc acggtgtgta tccttgatca gaaatgcatc agagagagaa
     2641 tcccggtgcg gatcaccacc gccgagacga atagccttct tatcaaaata ccgaaggcct
     2701 ggtgctgtct tccgtgtacc ggcaacccgt acatgatcgt ttaccacgga cgcacgtatc
     2761 tgcgcagtcg cggtagccgt tgcaatcccg ctcatccgcc cgagaagatt cagtgcagtc
     2821 cgctctaccg caagaattac ctgcacagat ccggcaagtg acaaaatcct cgctccagca
     2881 gacacacgcc tgccgtcctc agaaatgacc gtcaccgaga caccaaagtg ctcaaacagc
     2941 cagacacact cctcaacacc ggcaagaacc atatcctccc gtgcctcaat ccgggcggat
     3001 acagtactgt cgggaactaa cgcaagagtg gtgatatcac ctgcgggaac atcctccgca
     3061 aaaaaagaaa gcagctgatc ggtaggaagc attacttcga cgcctcaatc atacgctcaa
     3121 tcgccgagcg ggcccggttt gcaatctcat ccgcaaccac aaccggatac accccgtcac
     3181 gaagcgtcac atacagatcc gaaagatcaa caaccttcat actcgggcag actgctgtct
     3241 ccatcacatg gaacacagta tccggatacc gcttttcaag cgggcggagg attcctttct
     3301 ccgtcagaat cacccattca tccgcagtcc cgcagttccg taccatatat ccggtcgagc
     3361 cgacaagatc acacgcctgc tgcacctcgg aactgcactc cggatggcag ataaccgttg
     3421 cattcggata cattcggcgt gcctcttcca catcgcgaac cgtaaagttg tgatgtgttg
     3481 gacatccgcc attcgccgga accggaatga tctccttatc cggcaactgg cgctgaaccc
     3541 atcccgccag gtttgcatcc ggaccaaaca gaattctctt ttctggaaga gacgctgcaa
     3601 tgactgcagc attcgccgaa gtacaggtaa tatcacactc cgctttcgtg gcagcgtttg
     3661 agtttacgta cactacaaac ggggatccgg gatgctctgc ctttgcatgc cgtaccatct
     3721 ccggagtcag ctgatctgcc agcggacatc cagcctcagg acgaggaatc agtacagtct
     3781 tttccggcga aagaatcttt gccgtttctg ccatgaagta cactctgcag tgaaccagcg
     3841 ttgactcctt tgcctccttc gcaagccgag caagctccag cgagtccccg cagatatcgg
     3901 caacatcctg aatctccgcc ggctgataat tatgggcaag aattattgca tttttctctg
     3961 cggcaagttt ttttatctgc tgctcgtaat tcatgcaccg atcaccagag gattatcaag
     4021 atttttcaga agggtgagaa tcgacagagc cgcaagataa ctggtcgcag gattgtcagg
     4081 agagggaagg tttctgatcc tgatatacgc ctcaccgaac tccccctcaa agaatatctc
     4141 atgcatattg cgatcctctt ccggatccat ccacaactcc acatcaacat cacggccgca
     4201 ggcaatcgac agtgactcag ccacattcgt gttcttcgga tactgtctga cacagtcgtg
     4261 ggctttgccg gagaacagct gtgtacgttc ggttgcttcg cgggaaagcg attttgggtt
     4321 cttggttgta cggagcacaa acttatcgac gcggctgatc tggccgatac ggatgttgtc
     4381 aagtcccatc actgcaccgg agggaatatg gatcttccgc ttcaggctgc gggcggtttc
     4441 cacaagatgt gcccggaact ctttgtctgc gagtgccccg acactcatga tgacaatatc
     4501 tttgcctgac tccagcacct cctgtgcgtg ttcggtggca gcaacaacgg atgcagcctc
     4561 aacaataata tcggttggtt cggcaaggaa agcagaaaag tctgaaaacg gttttgcgtt
     4621 gaactcgcga gcaaactcct ccacacgtgc cggaaccaca tcatacacag ccgttatttt
     4681 gaagttttcc tgacctgcag caatgacgcg gccgatattt ccgcatccaa gcagaccgac
     4741 ggtgatcata ctactaaata tcagagtgta cgtagttaag gattatgcca aaccccagac
     4801 ctccggggaa tgatagtgat gttgaaaaga tgtggttaaa acaaaatggg gagatgaaaa
     4861 atccaaatca ggatttttct tccggctgat actccagaat atcaccaggc tgacaattga
     4921 gtaccctgca tattgcttcc agggtggaga accgaattgc acttatctta ccggttttga
     4981 ttttggaaag attgacattg gtcacgccga ccttctcgga aagctcgttt aacgacatct
     5041 ttctgtccgc cataacgcga tcaagtcgga gaataatggg catgggtacc tcagagagtt
     5101 tcatcagcct gcttttgcag gagtgttccg tattcaaata tgtgtgcaag acagtataaa
     5161 accgcagcaa cgatcagccc ctcaatacta agataaccaa cagtaacgct gattcccgta
     5221 atataccaac caattgagtc tatgatgacc gggaccacac acagaagggc catacagata
     5281 gccacatgtt tcagactgcg ggcattggcc tgggtaaacg gcacaccgct ttcgcggatc
     5341 tccgcaaaga tcctcgctgc cgcccagatc attccagcaa aggttagaag ataaactgta
     5401 gctgatacta ggaatatagc tgatgcctga ataaatgcgg ttgtcggatc cattccagcg
     5461 ggaacagatg ttcctacccc aaatgaactg agaacatcct cggaaacgat ccagtgtgag
     5521 aattctgcag gtccccctac agtatagata catgcggtaa gcgcagcggt aaggaagagc
     5581 agtacgatca caaaaaccac caggatcttg ccgatcgtca ggacaatgcc aacattcttt
     5641 gcgttttgtt ttatttttga ctgaatttca acttcgttca tatagatacc tcgtttagga
     5701 tggatgggaa gaggcatgat gtacgacaaa atggagatac aggagaatga ggagaactaa
     5761 actccccgct gttccaagaa ctaacaggga aaggaggtgg aaactttcca ctgtcagact
     5821 acagtaccca aacatcacac tccatccaag aacaatcaga actgcagcaa gaccagctgc
     5881 agcggtgacc gttgcaagtg acaggtaata gga
//
LOCUS       DALUAJ010000202         2880 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_39674,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000202 DALUAJ010000000
VERSION     DALUAJ010000202.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39674"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="O0X37_04290"
     CDS             <1..352
                     /locus_tag="O0X37_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HJK62172.1"
                     /translation="STWRPERGDDELAELLRQRMMQLQQQQMAEQEQAQRQQQMQAQV
                     QSVLMLVMETEARERLNTIKLTKPEFAAAVEQQIVALAQSGRLRQKITDDQLKQLLSQ
                     IVPQKKEFNIRRVG"
     gene            349..942
                     /locus_tag="O0X37_04295"
     CDS             349..942
                     /locus_tag="O0X37_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha hydrolase"
                     /protein_id="HJK62173.1"
                     /translation="MKAGVLYSGGKDSTLAAVLLARDYEVELITFVFDPNHAVPSIEA
                     AAKATGFPWKKQVFAPGFLDEVVNMIVEDGHPANAINEIHRRSLCALAQEYEVVADGT
                     RRDDRVPMRTQSEVQSLEMKYGVSYVRPLLGIGKREIIRLCERSFEIAYGETGTIPNG
                     DFESEIRAEMVRRGIDYVPLFPKNHEQSLIIRKKNGD"
     gene            945..1097
                     /locus_tag="O0X37_04300"
     CDS             945..1097
                     /locus_tag="O0X37_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L39e"
                     /protein_id="HJK62174.1"
                     /translation="MSKLTKARKIRLAKATQQNRRVPAWVMIKTKRNVVSHPCRRNWR
                     RSTLKV"
     gene            1102..1365
                     /locus_tag="O0X37_04305"
     CDS             1102..1365
                     /locus_tag="O0X37_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31e"
                     /protein_id="HJK62175.1"
                     /translation="MVEAQKEQVYVIPLRDVKRVPCYKRANAAIKDIRSYLEHHMKSD
                     DVKLDKSINEAVWARGSQKPPRRIRVRAMKFEDGQVQAELAEE"
     gene            1366..2034
                     /locus_tag="O0X37_04310"
     CDS             1366..2034
                     /locus_tag="O0X37_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-6"
                     /protein_id="HJK62176.1"
                     /translation="MDHTLSLNGDPNIGVFARVFEDIAFVPLDSPEEFRTKIAEALDV
                     EVIPTFIQGSSVIGLLLTGNSRGFVVSGLIHDSELDLLQEYGDVLLLGEEMNAAGNVI
                     LTNDSFAVVHPDMSSEMRRMVGEFLKVETIPMSFGGVGTVGMAGACTNAGVLLPARSS
                     PDELVHLENSLPTADVSIGTGSVNMGSGLIGTGLLVNSKGYLAGNATTGFELGRIEDV
                     FGFL"
     gene            2046..2231
                     /gene="rpl18a"
                     /locus_tag="O0X37_04315"
     CDS             2046..2231
                     /gene="rpl18a"
                     /locus_tag="O0X37_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18Ae"
                     /protein_id="HJK62177.1"
                     /translation="MPKFEVKGTFKNDGQMKPYTKIVDAPSERLAGEFTLATIGSKHR
                     LERKYITVDSVKAINGE"
     gene            2221..2670
                     /gene="pfdA"
                     /locus_tag="O0X37_04320"
     CDS             2221..2670
                     /gene="pfdA"
                     /locus_tag="O0X37_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prefoldin subunit alpha"
                     /protein_id="HJK62178.1"
                     /translation="MASNANQATLEQEVQALRSYAAEYSQQFELLSQQLRFIETARAE
                     SLASIEALEALAAADGEVSTLLGLGGGVSVRATVVDSKKMLVGIGAGVTVEKSTEEAI
                     SFLRDRITEMDASGKRLSESLGKLQSQMAAVEQRMQEIYASAQQQNR"
BASE COUNT          731 a          661 c          772 g          716 t
ORIGIN      
        1 cagtacgtgg cgaccagaaa ggggagacga tgaactcgca gaacttctcc ggcagcgtat
       61 gatgcagctc cagcagcagc agatggctga acaggaacag gcccagcgcc agcagcagat
      121 gcaggcacag gtccagtctg ttcttatgct ggtaatggaa actgaagccc gcgaacggct
      181 gaacactatc aagctgacca aacctgagtt tgccgcagca gtcgagcagc agattgtcgc
      241 tcttgcccag tcaggacgcc tgcgtcagaa aattactgat gaccaactca agcagttgct
      301 ttcgcaaatt gtaccgcaga aaaaggaatt caatatccgc agggttggat gaaagcgggt
      361 gtgctctaca gcggcggcaa agacagtacg cttgccgcag ttctgcttgc gcgggactat
      421 gaggtggagc tgataacctt cgtattcgat ccaaaccatg ctgtcccttc cattgaggcg
      481 gcggcaaagg caaccggttt cccctggaag aaacaggtct ttgcgccagg tttccttgat
      541 gaagtcgtca acatgattgt tgaggacggg catcctgcaa atgccatcaa tgaaatccac
      601 cgacggtctc tctgtgcact tgcacaggaa tatgaggtgg tcgccgacgg aacccgtcgt
      661 gatgaccggg tcccgatgcg gacacagtcc gaagtacaga gccttgagat gaagtacggg
      721 gtcagctatg tgcggcctct gcttggcatt ggaaaaagag aaattatccg tctgtgtgaa
      781 cggtcctttg agatcgccta cggcgaaacc gggacgattc caaacggtga tttcgaaagt
      841 gagatcaggg ctgaaatggt tcgccgggga attgactatg tgccgctctt cccgaaaaac
      901 catgaacagt cgttgatcat tcgaaaaaag aatggtgatt aaccatgagc aaacttacta
      961 aagctcgtaa gattcgtctt gcaaaggcaa cccagcagaa ccgccgtgtt ccggcctggg
     1021 tcatgatcaa aaccaagcgt aacgttgtgt ctcatccgtg cagacgcaac tggagacgca
     1081 gcactctgaa ggtgtaagaa aatggtagaa gcacaaaagg aacaggtata tgttattccg
     1141 ctccgtgatg tcaagcgtgt cccgtgctac aaacgtgcaa acgctgcaat caaagacatc
     1201 cgcagctacc ttgagcatca catgaaaagt gatgacgtta agcttgacaa gagcattaac
     1261 gaagcagtct gggcacgtgg atcccagaaa ccgccaagac gcattcgtgt tcgtgcaatg
     1321 aagttcgaag acggacaggt ccaggcagaa cttgctgagg aataaatgga ccacaccctg
     1381 tcactgaatg gagacccgaa catcggtgtc tttgcccgtg tctttgaaga tattgcattt
     1441 gttccgctcg actctccgga ggagttcaga acaaagatag cggaagctct tgatgtagag
     1501 gtcattccta cctttattca gggatcatcc gtgatcggtc ttctcctcac cggcaattcc
     1561 cgcggttttg ttgtttccgg tctcattcac gattcagaac ttgatcttct gcaggagtat
     1621 ggtgatgtcc tgcttctcgg tgaagagatg aatgcggcag ggaacgttat tcttaccaat
     1681 gattcattcg cggttgtgca tccggacatg tcgtctgaga tgcgcaggat ggttggcgag
     1741 ttcctgaaag tcgagacgat tcctatgtca tttggcggcg ttggaacggt tggtatggca
     1801 ggagcctgta caaatgcagg tgttctgtta cctgcccgga gcagtcctga tgagctggtt
     1861 catctcgaaa acagtcttcc gacagcggac gtttccatcg gtaccggatc tgtgaatatg
     1921 ggttccggtc tgattggaac tggtttgctc gtgaacagca agggttatct ggcaggaaac
     1981 gcgaccacgg gttttgaact cggtagaatt gaagatgtat ttggatttct gtgaggtgaa
     2041 ataatatgcc gaaattcgag gttaagggca cgttcaaaaa tgacggacag atgaagccct
     2101 acacaaaaat agtcgatgcg cccagtgagc gtcttgcagg agagtttact cttgcaacga
     2161 ttgggagcaa gcaccggctt gaacgcaaat atattactgt tgattctgta aaggctatca
     2221 atggcgagta atgcaaatca ggcaacgttg gaacaggagg tacaggccct ccgttcctat
     2281 gccgcagagt attcccagca gtttgagctt ctgtcccagc agttgaggtt tatcgaaacg
     2341 gcacgcgccg agtctcttgc ctctattgag gcactagaag cactggctgc cgcagacgga
     2401 gaggtttcca ccctcttagg cctgggcggc ggtgtgtccg ttcgtgcaac tgtagtggac
     2461 tcaaagaaaa tgcttgttgg gattggtgca ggtgttacgg ttgagaagtc aaccgaggag
     2521 gctatctcct tcctgcgtga tcggatcacc gagatggatg catccggtaa acggctttct
     2581 gagtctcttg gaaaactgca gagtcagatg gcagcagttg agcagcgtat gcaggagatc
     2641 tatgcttctg cacagcagca gaaccgctga catttcctct tttattcatt cagtgtgtat
     2701 tttcttatcg gtccattcat tttcgttggg agattttcac aaatgcaaat tcatgaaata
     2761 cccgtatctg taagggaaga atattcttca atcatctaaa atgtctttgt ctctgttttt
     2821 gtattcttcg gtactcacgt tttttctctt taaaatgtcg gataatatct gattttattc
//
LOCUS       DALUAJ010000203         2882 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_39736,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000203 DALUAJ010000000
VERSION     DALUAJ010000203.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39736"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="O0X37_04325"
     CDS             complement(<1..180)
                     /locus_tag="O0X37_04325"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HJK62179.1"
                     /translation="MTDKPVRLGFVVAEFNRDLTYMMEMEAEEHAKFLGASVSDRIYV
                     PGAYDMPLAIRKLLKK"
     gene            complement(262..1332)
                     /locus_tag="O0X37_04330"
     CDS             complement(262..1332)
                     /locus_tag="O0X37_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HJK62180.1"
                     /translation="MSAAPAPGILAIGFAVLAAVLYGISSPVSKVLLDSVPPALLAAL
                     LYLGAGVGMAVVSLVQTSRRQTVREASLGKKDLPYVIGMIVLDIAAPILLMFGLTMTT
                     AANASLLNNFEIVTTSVIALLVFREAIDRRLWFAILLIVCASIILTVEDMSSFSFSAG
                     SVLVLLACVAWGFENNCTRMLSLKDPVQIVTIKGFGAGGGALLIALVVQETSADTGGI
                     LAALLLGFFAYGLSIYFYVLAQRSLGAARTSAFYAVAPFIGVGLSFVIFQTPLTVSFA
                     VATVIMIAGAYFAATGGHQHRHIHLPQMHEHSHSHDDGHHTHVHNPPVTGEHSHMHTH
                     EILEHSHPHAPDLHHTHDHKSR"
     gene            complement(1481..2092)
                     /locus_tag="O0X37_04335"
     CDS             complement(1481..2092)
                     /locus_tag="O0X37_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62181.1"
                     /translation="MYRDDAVSEVVGFSLILGMVILALGLLSVHATPQITEDLEYSQN
                     DNVLQQFAAFKYDLDLFLAAENTGVYGRTVFTFVPEQTSFSVFSFSDLTPGRISMERG
                     VPAFQNNGTDYYPVFFTYQSANRYAENILLELRAGTLTVDPSGLTIPAFGTEDSASAI
                     VVTDSSFVPWVVSGVEQVIVEYRLEHVLHSDSGTLYVFDFSMR"
     gene            complement(2092..2775)
                     /locus_tag="O0X37_04340"
     CDS             complement(2092..2775)
                     /locus_tag="O0X37_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62182.1"
                     /translation="MSSYADDGISEVVGFICILVLITVVLAVWAAGSVPAGELWEDQI
                     SDETAAVQFSDMKLAMDLLWITGDPGDIRSVILDAGSFSVGTSDAVIHVSRNDTAAAS
                     YTPLKLVYLPGPAATNRFALAVDSGAVIQMTRETTLMLLPPSVVADRDNVCITVPVLT
                     SPSVEIVKADPLAVTFHLQNIRENRFDNTTISVTGDPLLWNALSRELGDAVRYNLTVR
                     EVLYRVEVA"
BASE COUNT          827 a          697 c          735 g          623 t
ORIGIN      
        1 ctttttcaga agtttgcgaa tggcaagggg catatcatag gcgcccggca catagatccg
       61 gtccgaaact gacgcaccaa ggaactttgc gtgctcctct gcctccatct ccatcatata
      121 ggtaagatcg cggttaaact ctgcaacaac gaatccgagt ctgactggtt tgtcggtcat
      181 gataatctca gtacaattct gtcgcaaaag tatatagtgg ttcacaaaaa cgtcacaact
      241 caaaaatgag ttgacaacat ctcagcggga tttgtgatcg tgcgtgtggt gaaggtccgg
      301 tgcgtgcgga tgactgtgtt ccaggatttc atgcgtatgc atgtgcgagt gttctcccgt
      361 taccggcggg ttatggacat gggtgtggtg accgtcatca tggctgtgac tgtgttcatg
      421 catctgcggc aggtggatgt gccggtgctg atgtccgccg gttgcggcaa agtatgctcc
      481 cgcaatcatg atcacggtgg caacggcaaa agagaccgtc aacggagtct ggaatatcac
      541 aaacgagagc ccgacaccaa tgaacggggc aaccgcatag aacgcacttg ttctcgctgc
      601 accaagtgac cgctgggcaa ggacatagaa atagatcgaa agaccgtatg caaaaaatcc
      661 gagcagaagt gcagcaagaa tgcctccggt atccgcgctc gtctcctgta caacaagagc
      721 gatcagaagt gctccgcctc ctgcgccgaa ccctttgatg gttacaatct ggaccggatc
      781 ctttaacgag agcatccgcg tacagttgtt ctcaaaaccc caggcaacgc aggcaagcag
      841 aacgagaacc gaacctgctg agaaggagaa actgctcatg tcctcaacgg tgaggatgat
      901 acttgcacag acgatgagaa gaatggcaaa ccagagtctc cgatcaattg cttcccggaa
      961 cacaaggaga gcgataacag atgtggtgac aatctcaaag ttgtttaaga gggacgcgtt
     1021 tgccgcagtg gtcatggtaa gaccaaacat cagcagaatg ggagcagcaa tatcgagaac
     1081 aatcatcccg atgacgtacg ggaggtcctt ttttccaaga gaagcttcac ggaccgtctg
     1141 tctgcgcgat gtctgaacca gactgaccac cgccatccca acacctgccc caagatagag
     1201 aagagctgca agcagtgccg ggggcacact gtcaagcagt actttggaga ctggagagct
     1261 gataccgtag agaacagcgg caagtactgc aaatccaatg gcaagaatcc ccggggcagg
     1321 tgcagcagac atatgctgct aatactattt gcaatgatta catataattc cctgttagaa
     1381 tggatttttc aataattctg cggatttctt accaaaaatt tcagaacatg cagcacgtat
     1441 tacgaacgaa aaaaatgtgt gaaaagagta aacgatttca tcaccgcatg ctgaaatcaa
     1501 acacatagag tgtgccggaa tcgctgtgca gaacgtgttc gaggcggtac tcaacgatca
     1561 cctgttcgac cccggagaca acccatggaa caaaggagga gtcagtaaca acaatggcgg
     1621 atgcagagtc ctccgttcca aatgcaggaa tggtaagacc ggacggatct acagtgagtg
     1681 ttcccgcacg tagttcaagg agaatatttt ccgcataccg gttggcggat tggtaggtga
     1741 aaaacaccgg atagtaatca gtaccattgt tttggaatgc aggaacacca cgttccatac
     1801 tgatgcgtcc gggagtgaga tcagagaagg aaaatacaga gaacgaagtc tgttccggta
     1861 caaaagtaaa gacggtccgg ccgtaaacac cggtattttc tgctgcaaga aaaagatcca
     1921 gatcatactt gaacgctgca aactgctgga gaacattatc attctgggag tattccagat
     1981 cctctgttat ctgcggcgtg gcatgaaccg acaggagccc aagggcaagg ataaccatac
     2041 caagaatcag ggagaacccg accacctctg atactgcatc gtcccggtac atcaggcaac
     2101 ctcaactcga tagagaactt cccggacggt caggttgtat ctgacagcat cgccaagttc
     2161 ccgggacaat gcgttccaaa gaagggggtc tccggttaca gagatggttg tgttgtcaaa
     2221 acggttttca cggatgtttt gtagatgaaa ggtaaccgca agaggatctg ctttgacgat
     2281 ttccaccgac ggagaggtaa gtactggaac tgtgatacaa acattgtccc tgtctgcaac
     2341 aacggacgga ggaagaagca tcaacgttgt ttcgcgtgtc atctgaatta cagccccgct
     2401 gtcaaccgca agagcaaacc ggtttgtcgc tgcgggtcct ggcagatata ccagtttgag
     2461 cggggtgtag gacgcagccg cagtatcatt gcggctgaca tggatcactg cgtccgaagt
     2521 tccaacacta aaggatcctg catcaagaat caccgaacga atatctccgg gatctccagt
     2581 aatccacaga agatccatgg cgagtttcat atcagaaaac tgtaccgcag cggtttcgtc
     2641 actgatctga tcttcccaga gttcaccggc gggaacagac cctgcggccc acactgcaag
     2701 aacaactgtt atcagtacaa gaatgcagat gaatccgaca acttcggaga tcccatcatc
     2761 cgcatatgac gacatagcag aacatttcca aaaatcacaa tatacttttg catagtctct
     2821 gacgctgcaa tcatcagtta tttcagatgc gaacggttat ctgccatgcc atcgggagag
     2881 ac
//
LOCUS       DALUAJ010000204         3131 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_39740,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000204 DALUAJ010000000
VERSION     DALUAJ010000204.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39740"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(17..646)
                     /gene="thiE"
                     /locus_tag="O0X37_04345"
     CDS             complement(17..646)
                     /gene="thiE"
                     /locus_tag="O0X37_04345"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HJK62183.1"
                     /translation="MYSLYVVTDEKLSRGLTHAEIARHAVAGGANVIQLRDKEKSSRE
                     LYEIACEIREICCDRALFFVNDRLDIALAAKADGVHLGQDDLPIEAARQIAPRNFLIG
                     ISVGNVDEARAAEAAGADYVAVSPVFATASKTDAGAGHGIKAIRDIRAAVKCPVIGIG
                     GINADNAAELIDAGLDGVAVISAVVSATDVTAAAENLSAIVRNAKERRK"
     gene            complement(651..1445)
                     /gene="thiM"
                     /locus_tag="O0X37_04350"
     CDS             complement(651..1445)
                     /gene="thiM"
                     /locus_tag="O0X37_04350"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HJK62184.1"
                     /translation="MQKYAEILESVRTASPLVHQITNYVTVNDCANITLCIGASPVMS
                     HAPEDVIDMTKIANALVLNIGTLDPGQIEGMLVAGRVAAERNIPIILDPVGAGATPYR
                     TKTAQQLIEELPIAVIKGNAGEIATLAGVVATVRGVDSGDVAGDKKTIVKNLAAELGC
                     VVIMSGEEDLISNGTRVAGVRNGVPLMGRISGTGCMASAVTGAFAAVAPDTMDGCIAA
                     MVSLGIAGETAANTVAGPGSFKPAFLDAVAALTPEQVAGHAKVTEY"
     gene            1520..2749
                     /gene="hmgA"
                     /locus_tag="O0X37_04355"
     CDS             1520..2749
                     /gene="hmgA"
                     /locus_tag="O0X37_04355"
                     /EC_number="1.1.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase (NADPH)"
                     /protein_id="HJK62185.1"
                     /translation="MDEQLAKIRSGDLKLYALEKLMPADEAVALRRKYIEEETSADLT
                     PIGKYTIPIDRVVTRNVENMIGCVQIPVGVAGPVVVNGEYAKGEFWLPLATTEGALIA
                     SINRGCGAIRKAGGAEVRILRDGMTRAPVFAAKSVAHATEVARWAEDNLLLLKVAAEE
                     TTHHGELIGLTTYVVGTNVFVRFEFDTKDAMGMNMATIASEAAAKVIEAETGARMVST
                     SGNMCCDKKPSAVNVIEGRGKSVVAGVFLSDDLVAAQFKTDARTLAEVNTRKNLVGSA
                     RAVSLGFNAHAANVIAAMFLACGQDPAHVVEGSNAITTVDAVPGGVYVSVSLPSLQVG
                     TVGGGTGIATQQACLSMLGVAGGSEIPGDHAKAFGEIVATAVLAGELSLLGALGAQHL
                     AKAHQELGRGGAAGVGQ"
BASE COUNT          601 a          699 c          926 g          905 t
ORIGIN      
        1 caccactgct cctttgtcat ttccgcctct cctttgcatt acgcacgatc gctgaaaggt
       61 tctctgccgc tgctgttaca tccgtggcac tgaccaccgc ggagataact gcaactccgt
      121 caagtccggc atcaattaat tctgcggcgt tgtctgcgtt gattccaccg atgccgatca
      181 ccggacattt gaccgcggca cgaatatccc tgatcgcctt aataccgtgt ccagctcccg
      241 cgtccgtttt tgaagcagtt gcaaacaccg gactcacagc aacgtagtcc gctcccgccg
      301 cctctgccgc tcttgcctcg tcaacgtttc ccacagagat tcctatcaaa aaattccgtg
      361 gtgcaatctg tcttgccgcc tcaatcggca gatcgtcctg accaagatgc acgccatctg
      421 cttttgcggc aagggcaatg tcaaggcgat cgttcacaaa gaatagggca cggtcacagc
      481 agatttcccg tatttcacag gcgatctcgt acagttctcg cgaggatttt tccttgtccc
      541 gcaactgaat tacgtttgct cctcctgcaa cagcatggcg tgcgatctcc gcatgtgtga
      601 gaccgcgtga cagtttttcg tcggtgacta cgtagaggct gtacattggt ttagtactcg
      661 gtgacttttg cgtgaccggc aacctgttcg ggtgtgagtg cagcgactgc gtcgaggaat
      721 gccggtttaa atgatcctgg acctgcaacc gtgtttgccg ctgtttctcc ggcaatgccg
      781 agtgagacca tggcggcaat acagccgtcc attgtgtcgg gtgcaaccgc agcaaatgct
      841 ccggtaaccg cggacgccat acatccggta ccggagattc ttcccatcag gggaacacca
      901 ttcctgacac ctgcaacacg ggttccgttg ctgatcagat cctcttcccc gctcatgatt
      961 acgacgcacc cgagttcggc tgcaaggttt ttgacgattg ttttcttatc tcctgctaca
     1021 tcgccggagt ccacaccgcg aaccgttgcg acgacacctg cgagggttgc gatctctccg
     1081 gcgtttcctt tgataacggc tattgggagc tcttcaatca gctgctgtgc ggtttttgtc
     1141 cggtacgggg ttgcgcctgc tccgaccggg tcgaggatga ttggaatatt tcgttcagcg
     1201 gcaactctgc ctgcaacgag cataccttct atctgtcctg ggtcgagggt accgatgttg
     1261 agtacgaggg cgtttgcgat ctttgtcatg tcgatgacgt cttcgggggc gtgagacata
     1321 accggtgatg ctccgataca gagcgtgatg ttggcacagt cgttgacagt gacgtaattc
     1381 gtgatctggt ggacgagcgg tgatgctgtt ctgacggatt caagaatttc tgcgtatttc
     1441 tgcatggaac acctttttgg taatattagg tctgcggtca gaaaaatgtt ttagtgtgcg
     1501 tgctgaatgg tatattatta tggatgagca gcttgcaaag atccgctccg gcgatctgaa
     1561 actgtacgct ctggagaaac tgatgccggc agatgaggct gtggctctgc gcaggaaata
     1621 tattgaggag gagacttccg ccgatcttac tccgattgga aagtatacta ttccgattga
     1681 tcgtgtggtt acgcggaatg ttgagaatat gatcggttgt gtgcagatcc ctgttggtgt
     1741 tgcaggaccc gttgttgtta acggcgagta tgcaaaggga gagttctggc tgccgcttgc
     1801 aacgacggaa ggagcactta ttgcgtcgat taaccgcggc tgcggtgcga ttcggaaggc
     1861 tgggggtgcg gaggtgcgga ttctgcgtga cggtatgacg cgagcaccgg tgtttgcagc
     1921 aaagtcggtt gctcatgcta ctgaggttgc ccggtgggca gaggataacc tgttgctgct
     1981 gaaggttgca gctgaggaga caacgcatca tggtgagttg atcggtctta cgacgtatgt
     2041 ggtggggacg aacgtgtttg tgcggtttga gttcgatacg aaggatgcga tgggaatgaa
     2101 tatggcaacg attgcgtccg aggcggcagc gaaggttatt gaggcggaga cgggagcacg
     2161 gatggtgtca acgtctggaa atatgtgctg tgataagaag ccgtcagctg tgaatgtgat
     2221 cgaggggcgg gggaagtccg ttgttgccgg ggtgtttttg tcggatgatc ttgttgcggc
     2281 acagtttaag acggatgccc ggacacttgc ggaggtgaat acacggaaga atctggttgg
     2341 ttctgcccgt gcggtgtcgc ttgggtttaa tgcgcatgcg gcgaatgtga ttgcggcgat
     2401 gtttttggcg tgcggccagg atcctgcgca tgttgttgag ggaagtaatg cgattacgac
     2461 agttgatgct gtccctggtg gtgtgtatgt gtcagtgtct ttgccgtctc ttcaggtggg
     2521 aactgttggc gggggtacgg gaattgcaac ccagcaggcc tgcctgtcga tgcttggtgt
     2581 tgcgggcggc agcgagatcc cgggagatca tgcgaaggcg tttggggaga ttgtggctac
     2641 tgcggttctt gctggtgagt tgtcgcttct gggcgcactt ggtgcacagc atcttgcgaa
     2701 agcacatcag gagcttggac gcggtggtgc tgcgggcgtc gggcagtaac ttctctttct
     2761 ttttttgatt tttgtttggt aggatttagt gtttgtttct cggaaggatt caatctcctg
     2821 tttgtctttt tgtagtactt tgtttgtggt gttttgtgta tttttgttat ttgggatcgt
     2881 ctgtttcgtt gattgatgaa atttttctgt gtccaaaatt ccaagaattt tgtgtatttt
     2941 taggatatta gttggtgctg tatctgtggt gatgatgttt tctgtgcttt atgtgatggt
     3001 tttgtgtttg ttgttttttg atatttgcca tattttctgg tatgtatgcc tgttgtgatt
     3061 gaatcctcat ttaggaggaa ttacacttga aaaacacgaa aataatggca gttctcgtcg
     3121 ttctgctcgc a
//
LOCUS       DALUAJ010000205         1816 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_39825,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000205 DALUAJ010000000
VERSION     DALUAJ010000205.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39825"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="O0X37_04360"
     CDS             complement(<1..796)
                     /locus_tag="O0X37_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HJK62186.1"
                     /translation="MAQYTCGVCGYTYDEAAGVLFSELSDSWLCPVCSAGKQQFTLTS
                     SPAKETGYTGTSVMDEIHYMAETGRSITEPMETLMPVPSFDEILLLGAQLSRLPLDDD
                     AQVNVRTVIGKHAKQPMVLETPVFVSHMSFGALSREAKIALAKGSAAAKTAMCSGEGG
                     ILPEEKEASYRYIFEYVPNRYSVTDKNLSEADAIEIKIGQGTKPGMGGHLPGAKVTEE
                     IARIREKPVGEDVLSPSHFPGITTPADLKCLVDELRNRSGGRPIGVK"
     gene            complement(818..1360)
                     /locus_tag="O0X37_04365"
     CDS             complement(818..1360)
                     /locus_tag="O0X37_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HJK62187.1"
                     /translation="MANLKGTKTEANLQAAFAGESQARNKYNYYASKARKDGYEQIAE
                     LFEETANNEKEHAKIWFKLLHGGAVPDTLTNLADAAAGENYEWTDMYAGFAKEARAEG
                     FEDIAKLFEMVADIEKHHEERYLKLLANINEGIVFSRDGDMIWQCGNCGHLVIGKSAP
                     EVCPVCDHPKAYFRIEARNY"
     gene            1528..>1816
                     /locus_tag="O0X37_04370"
     CDS             1528..>1816
                     /locus_tag="O0X37_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62188.1"
                     /translation="MSELMQKIERATSSTFFDQTGRNEGSGRCCKIMRILLEGTCSFD
                     CAYCGVCTKKNGISFTPQELARGFLSLHNEGRVGGLLLSTGIPRGDVDLGME"
BASE COUNT          356 a          487 c          501 g          472 t
ORIGIN      
        1 tctttacgcc gatcggccgt cccccgcttc tgttccgcag ttcatccacc agacacttca
       61 gatccgcagg cgttgtaatt cccgggaagt gggacgggct tagcacatcc tcaccgaccg
      121 gtttttccct gatacgtgcg atctcttcgg taacctttgc gcccgggaga tgaccgccca
      181 ttcccggctt tgtcccctga ccgatcttaa tctcaatcgc atcagcctcg gaaagattct
      241 tgtcggttac gctgtagcgg tttggcacat actcaaagat gtaccggtag gatgcttcct
      301 tctcttcggg aagaataccc ccttcgccgc tgcacattgc agtctttgcc gcggccgacc
      361 cttttgccag ggcaatcttt gcctcccggg agagtgcacc aaagctcatg tgcgaaacaa
      421 agaccggggt ttccagaacc atcggctgct ttgcatgctt tccgatcacc gtccgcacgt
      481 tcacctgtgc atcatcgtcc agcggcagac gggaaagctg ggcaccgagc agcagaatct
      541 cgtcaaatga cggtaccggc atcagtgtct ccatgggctc ggtaatgctt cttccggtct
      601 ctgccatata atgaatctca tccattacgc tggttccggt gtatccagtc tctttggcgg
      661 gggaggatgt cagagtaaac tgctgttttc ccgcggaaca gacgggacag agccaggagt
      721 cggaaagttc tgaaaacaag acgcctgctg cttcatcata ggtatagccg cagaccccgc
      781 aggtgtactg tgccataata aaatctccaa aaaaaagtta gtagttccgg gcttcgatcc
      841 ggaagtatgc cttcgggtga tcgcagaccg ggcagacctc gggagcggat ttgccgatga
      901 ccaggtggcc gcagttgccg cactgccaga tcatatcacc gtcacgggag aagacgatgc
      961 cctcattgat gtttgccagg agtttcaggt accgctcttc gtggtgcttc tcgatgtctg
     1021 caaccatctc aaagagtttg gcgatgtcct caaagccttc tgcacgtgct tcctttgcaa
     1081 agcctgcata catgtcggtc cactcgtagt tttcgcctgc cgcagcgtcg gcaaggttgg
     1141 tcagcgtgtc cggaactgca ccgccgtgca gaagtttgaa ccagatcttt gcgtgttcct
     1201 tctcgttgtt tgcggtctcc tcaaacagct cggcaatctg ctcatagccg tctttgcgtg
     1261 ccttggatgc gtagtagttg tacttgtttc ttgcctggga ctcgccggca aatgcggcct
     1321 ggagatttgc ttcggtcttg gttcctttca gatttgccat agtgatacaa cctcggaaat
     1381 gattggtatc ccttatgtta gggagtgcgt ggtaatgaat gtgacgagtg tccggagagc
     1441 cgggtcttct gcggtgtttt tgcgggaggg cattttcact ccgcacggta gggggtttaa
     1501 gtagtgtcct gccgacagta tggtgtaatg tcggagttga tgcagaagat cgagcgggcg
     1561 acaagctcta cgttctttga tcagaccggc cgcaacgagg gttcgggacg gtgctgcaag
     1621 atcatgcgga tactgcttga gggtacctgt tcctttgact gtgcttactg cggtgtctgt
     1681 acgaaaaaga acggtatcag tttcacaccc caggagcttg cccgaggctt tctttccctg
     1741 cacaatgagg ggagggtcgg cggtcttctg cttagtaccg gtattccccg cggcgatgtg
     1801 gatcttggta tggaaa
//
LOCUS       DALUAJ010000206         2937 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_39901,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000206 DALUAJ010000000
VERSION     DALUAJ010000206.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39901"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="O0X37_04375"
     CDS             <1..700
                     /locus_tag="O0X37_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HJK62189.1"
                     /translation="RKEIEALRAEINRCIGTETSQNTLGALETFRKDTADAIIAGELA
                     RARETLETQAKNCPAKEQCLPQFEKTLAMLLNSLKSGSISTAGIAEIRREYEEAQKIC
                     GYDHCTGCLLEADKLFAAYIRLLKHAGVYAGDSIGEQIQELADEAVVAWIGEPLANVI
                     RVKILRSLASEPKSFADLGKLTGLRGGNLQFHLEKLISAGMILQKGERKEYVLTARGH
                     DLLGAVAILMEKFG"
     gene            1201..2154
                     /locus_tag="O0X37_04380"
     CDS             1201..2154
                     /locus_tag="O0X37_04380"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HJK62190.1"
                     /translation="MDAYELVTRNTAEIVTEDELRQLLNKPTKRVYTGYEPSGEIHLG
                     HLVTINKLMDMKAAGFDVVVLIANLHAYLNRKGTFEQIKELAEYNKACIEACGLKGAE
                     FVLGTDVQLTPKYQTEVLTLCQEITLNRATRSMDEVGRAMDNPMVSQMVYPVMQVVDI
                     PTLGVDAAVGGIDQRKIHMLAREHLPSLGYKPPVCIHTPIVNGLDGQKMSSSKGNVVS
                     VADSPEDIKKKMKKAFCPPEVEGNPILQVFQYNIFPRVSEIVIRRPEKFGGNAEYQSY
                     AELEAAYAAGKIHPMDLKAACGDALTELLADAYAYVQSYKN"
     gene            2253..>2937
                     /locus_tag="O0X37_04385"
     CDS             2253..>2937
                     /locus_tag="O0X37_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HJK62191.1"
                     /translation="MQLTVLVDNNTAIDKYYLGEPGLSFYIEDDSEKILFDCGYSDVC
                     KINAERMGVDLSRITTLVFSHGHDDHTGGLAYLQTILPPAKIVAHPDTFREKRSNGKN
                     AGSIFCTEELAEKYQLYLSREPQKVSDHLTFLGEIPKLNDFEPRIQFGEYKSPEGFVP
                     DFVMEDSALVYEREDGIFIITGCSHAGICNIIAYAKNLFQKPILGIIGGFHLKEVNER
                     TKQVIGYLET"
BASE COUNT          847 a          710 c          716 g          664 t
ORIGIN      
        1 caggaaagag atcgaagcgc tccgagccga aataaaccga tgtatcggaa ctgaaacatc
       61 ccaaaacacc ttaggggcac tggaaacgtt ccgcaaagat acagcagatg cgatcattgc
      121 aggagaactt gcacgcgcca gggaaacgct ggaaacccag gcaaaaaatt gcccggcaaa
      181 ggaacagtgt ctgcctcagt ttgaaaaaac actcgcgatg cttcttaaca gtctgaaatc
      241 tggatcgatt tcaacggctg ggatcgcaga aatccgccgc gagtacgagg aggcccaaaa
      301 aatatgcggc tatgatcact gtacaggatg cctgctggaa gcggacaaat tatttgccgc
      361 ctacatccgg ctactgaaac atgcgggtgt gtatgcaggt gacagtatcg gtgaacaaat
      421 ccaggaactt gccgatgagg cggtggttgc ctggataggt gagcctctgg cgaacgtgat
      481 ccgcgtaaaa attctcagat cccttgcaag cgaaccaaaa tccttcgcag accttggaaa
      541 gctgaccggc cttcgcggag ggaatctaca gttccatctg gaaaaactga tttctgctgg
      601 gatgattctg cagaagggag aacgaaaaga gtacgtcctg accgcacgcg gacatgatct
      661 tttgggagct gttgccattc tgatggaaaa attcggataa tttttctttt tgtcactatc
      721 cggaaccatc taatcttttg gaacgataga ttccgtggac agcatgaccc agagataaca
      781 atattatgtc ctctgaagtt atggaattcc tcaaaaaacc cgctacgtat ctcgcatccg
      841 ttgacaagga cgaaaatccc cttgtacgtc caggtaaagg accatccaag agtagaagtt
      901 gcaacatttg atcccgagaa aaaatcctgg ctctatatct cagaaaatct ggtcttcacg
      961 gatgatgaag atatcaaaaa cgatgtgtat ctggcacatc tgttcatgaa aaaactcttc
     1021 agattctttg aatattcaaa gttttatgtg tccactcttg ccggaaatgc agtgagtacc
     1081 gaatgtgaac aaaactgcca aagtcctata ccctgaaggg agagagaaac ccactttttt
     1141 caacctgtct acactgtatc acacatcctt tatctcattg tgattccaac gtagtagaat
     1201 atggacgcat acgaactcgt cacccgtaac acggcggaga ttgtcactga ggacgaactc
     1261 cggcagttgt tgaacaaacc aacaaaacgg gtctataccg ggtatgaacc cagcggcgag
     1321 attcacctcg gtcacctggt gaccatcaat aaactgatgg atatgaaagc agcagggttt
     1381 gacgtcgtgg tcttgatcgc aaacctgcac gcatatctga accggaaagg aacattcgaa
     1441 cagatcaaag aacttgccga gtacaataag gcgtgtattg aggcatgcgg tctgaaaggc
     1501 gcagagtttg tactggggac cgacgttcag ctgacaccga agtatcagac cgaggtatta
     1561 accctctgtc aggagattac cctgaaccgt gccacccgca gtatggatga ggttggccgg
     1621 gccatggaca acccgatggt ctcgcagatg gtgtacccgg taatgcaggt tgtcgatatt
     1681 ccgacactcg gcgtggatgc ggctgtgggc ggaattgatc agcgtaagat ccacatgctg
     1741 gcccgcgagc atctcccaag cctcggttac aaaccgccag tctgtattca cacaccgatc
     1801 gtaaacggtc ttgacggaca aaagatgtct tcatcgaaag gcaacgtcgt ctcggtcgca
     1861 gactcgcctg aggacatcaa gaagaagatg aaaaaggcat tctgcccgcc tgaggtggaa
     1921 ggaaatccga ttctgcaggt gttccagtac aacatcttcc cccgtgtgtc ggagattgtt
     1981 atccgccgtc cggaaaagtt cggcggcaat gcagagtacc agagctatgc agaactggaa
     2041 gcagcctatg ccgcaggaaa gattcacccg atggatctga aagcagcctg cggcgatgcc
     2101 ctgacagagc tccttgccga tgcctacgca tatgtgcaga gctacaagaa ctgatcgtgg
     2161 taaaaactgc aaaatttttt cttttttcag gtgactgata ctgtgtgccg ggaaaggcct
     2221 catatgatcc tccggcgaat accattgtac atatgcagct aaccgttctc gttgacaaca
     2281 acaccgcaat cgacaaatat tatctcggag aaccggggct ttccttctat attgaggacg
     2341 acagtgaaaa aattctcttt gactgcggat actcggatgt ctgcaaaatc aatgctgaac
     2401 gtatgggagt ggacctatca cgcataacaa cattggtgtt ttcccacggc catgacgatc
     2461 acaccggagg acttgcatat ctgcagacaa tacttcctcc ggcaaagatc gttgcccacc
     2521 ccgacacatt ccgagaaaaa cgaagcaatg gaaaaaatgc aggctcgatc ttttgtacag
     2581 aagaactcgc agaaaaatat caactctatc tttcacgtga gccgcaaaaa gtaagtgacc
     2641 acctaacatt tctcggagag atcccaaaac tgaacgactt tgagccgcgg atacagttcg
     2701 gtgagtacaa aagtcctgag ggatttgtcc ccgactttgt aatggaggac tcggccctcg
     2761 tctatgagcg ggaggacggt atctttatta tcaccggctg ttcgcacgcg ggtatctgca
     2821 atatcattgc ctatgccaaa aacctgtttc aaaaaccgat tctggggatc atcggcggat
     2881 tccatctcaa agaggtaaac gaacggacta aacaggtcat cggctatctg gaaacac
//
LOCUS       DALUAJ010000207         4837 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_39964,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000207 DALUAJ010000000
VERSION     DALUAJ010000207.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39964"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="O0X37_04390"
     CDS             <1..295
                     /locus_tag="O0X37_04390"
                     /inference="COORDINATES: protein motif:HMM:NF020549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1894 domain-containing protein"
                     /protein_id="HJK62192.1"
                     /translation="GDGMGCLENMNYEILAKNCSFKEAREIIRENCSEKYEVPAGFKL
                     LDKPLLGIPPILVGIKDEKLVYGYTKPCHGTFVVVVEDPEGMDNVRKNGKPVW"
     gene            303..863
                     /locus_tag="O0X37_04395"
     CDS             303..863
                     /locus_tag="O0X37_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HJK62193.1"
                     /translation="MFTSLLSTFFIAVGLAMDAFSVSLAGGAVLKKDVFRTALVAGLL
                     FGFFQFAMPLIGYVIGVPITNLITPYGYWLVFFPFLFVGGKMVYDAAFGREECGIDLT
                     GLKVLLLLAVATSIDALAVGVSYALLGEEILLPAVIIGVVAFGFSAAGVFVGNRLGCV
                     LGNKMEILGGVILVLIGCKFLLENVL"
     gene            complement(919..2001)
                     /locus_tag="O0X37_04400"
     CDS             complement(919..2001)
                     /locus_tag="O0X37_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62194.1"
                     /translation="MDHRYLLLILVVLFLAVPATAVEYDNTLTVSFDGYADSSPAESY
                     ADGSTVVIPYGSTVWIGATQYPGGKWTVTVPNLYPVKPSGKEIIQLSFGNVQGLYSVT
                     YDVPSVYYTNGAPKTITLNLNIGDVGGDSYADSIYVKYPNRYTLIFSDEYPVLQEGNT
                     KGLFSFGDPYNAPYIRVISVTTNGSEISQNARIVLVPQSSANPIPTPSQPYTALYDMK
                     VENIPVDAIEQVDFLFGVPIGVVTDAGFDPRYDIQLLRYTDSWEKLETSYVGEGTGQQ
                     AGMDWYAAKSPGFSYYAVGFAENVTKLPEEIFTPTPTATVEVIPPPPPTATTEPSVPT
                     APEQSSPFVFTGIIALLGAAVLCLRR"
     gene            complement(2028..2525)
                     /locus_tag="O0X37_04405"
     CDS             complement(2028..2525)
                     /locus_tag="O0X37_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62195.1"
                     /translation="MKIPVAAGVLAVLVLITFSAGCIGDQTTDIRLGNQSIGSITVTP
                     VYENMFYNSSLGDKFNVKLEIFGMVFTKDNVTRAEADNISTSILDKDGGVNMSFINSS
                     GLITPNANDAESNLTSFMDEVFNMPLTNRDKNTTINDINWDDAFSRMQESFNRLAGLL
                     NQSGN"
     gene            2664..3788
                     /locus_tag="O0X37_04410"
     CDS             2664..3788
                     /locus_tag="O0X37_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HJK62196.1"
                     /translation="MAIYDIDQFLASKRAIRTRQVIIWHFKAYFAWLYGSSSANDLTA
                     ASIRYITEPRNHFDTLQKYCTYMRDAADTLPNSQHQRLTYILQYLQWNEIELKSAQTN
                     LLRGILPPNIEQHNDTPLTREMIAAILAHCDTILRAIILVQVSSGMRINEVIGLRHGD
                     ISIDTQIKAHIPAARMKSSQPHTYHISHEAWSAVEEWLKIRDTVTASGAKRTEKTLGR
                     KKSWDPDQIFPITTTTLYRKFRTALKNAGYYEICQETSRLTISFHAFRRYAFTRMCDH
                     MPEEIANSMIAHTTNRLNRSYLRPDLDQYYAKVEPYLNILAPAEYIELKGSTAEQVSD
                     LAKAVASQVAKNQELEEELEEIRTALGLYMRAEKPDPDCS"
     gene            3996..>4837
                     /locus_tag="O0X37_04415"
     CDS             3996..>4837
                     /locus_tag="O0X37_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HJK62197.1"
                     /translation="MPGWGDILQEIEGNFAHDVVRRKYLKQLADHTGRNVIAYYSGWL
                     QFYDGELYISDNDKNGFMETIRGLDRTKGLDLLIHTPGGDIAATESIVEYLQEMFKGD
                     IRVIVPQLAMSAGTMIACSAKVIIMGKQSSLGPTDPQIVIQTNAGPQQVAAQGLIEEF
                     STAVDECNRDPRKLPFWNIIISQYSPTLIGNCQKAIEWSCDLTRQWLLNSMFKDDMAA
                     SEIVDNIVISLSNHSDTKEHGRHLSATTCKKIGLKVQMMEEDQALQDAILAVHHAYIH
                     TLSAT"
BASE COUNT         1316 a         1097 c         1159 g         1265 t
ORIGIN      
        1 aggtgatgga atgggctgtc ttgaaaatat gaactatgaa attctcgcaa aaaactgtag
       61 ttttaaagaa gcgagagaga tcattcggga gaactgttca gaaaagtatg aggtcccggc
      121 gggcttcaag ctgctggaca aacctctgct tggaattccg ccaattcttg tcggaattaa
      181 agacgagaaa ctggtgtacg ggtacacgaa accctgtcac ggaacctttg tcgttgtcgt
      241 agaggatccg gaaggcatgg acaatgtgag aaagaacgga aaaccggtct ggtagttttt
      301 tcatgttcac ctcactgctt tccacttttt tcatcgctgt cggtcttgca atggacgcgt
      361 tctccgtttc gcttgcggga ggtgcggtac tgaagaaaga tgtcttcaga acagcactgg
      421 ttgcaggtct gctgtttggt tttttccagt ttgccatgcc gctgatcggt tatgtgatcg
      481 gtgtcccgat cactaatctc atcacgccgt acgggtactg gcttgtcttt ttcccgttcc
      541 tgtttgtcgg gggcaagatg gtctatgacg cggcttttgg tagggaggag tgcgggatcg
      601 atctgaccgg tttgaaggtt ctccttcttc ttgcggttgc tacaagtatc gatgcccttg
      661 ccgttggtgt gagttatgct cttcttggag aagagattct tcttcctgct gttatcatcg
      721 gtgtggtcgc ttttggtttt tctgcggcag gtgtttttgt tggcaacaga ctcggctgtg
      781 tgcttggaaa caaaatggaa attcttggcg gagtcattct tgttttgatc ggttgcaagt
      841 ttctgctgga aaatgttttg tgaactggca taccctattt ttagtaaaac ggattttgaa
      901 aaaagaaaac tggggatttt accttcgcag gcacagcact gcagctccca gcagggcgat
      961 gatacctgta aatacaaagg gtgaggactg ttcgggcgcg gtcggaacag aaggttctgt
     1021 ggtcgccgtg ggtggaggcg gtggtatgac ctcaacggtt gctgtgggag tcggcgtaaa
     1081 gatctcctcc gggagcttcg ttacgttttc ggcaaatcct actgcatagt aggagaatcc
     1141 cggtgatttt gcggcatacc agtccatgcc ggcctgctgc ccagttcctt ccccgacata
     1201 tgacgtttcc agtttttccc atgaatcggt gtaacggagc agctggatgt cgtaccgcgg
     1261 atcaaatcca gcatcggtta ccacaccgat cggaacacca aagaggaaat ccacctgctc
     1321 aattgcatca actggaatat tctcgacctt catgtcgtag agtgcggtgt acggctggga
     1381 tggtgttggg attgggtttg cggagctctg cggaacgagt actattcttg cgttctgtga
     1441 tatctcagat ccatttgtcg ttaccgagat cacccggatg tacggggcat tatagggatc
     1501 gccgaaactg aagagtcctt tggtgtttcc ttcctggaga accgggtact catcggaaaa
     1561 gatcagcgta tagcggttcg gatactttac gtagatggag tcggcatatg aatctccgcc
     1621 gacatcgcct atgttgaggt tcagagtgat ggtctttggt gcaccgtttg tgtaatagac
     1681 agacggcacg tcgtaggtga cggagtacag tccctgaaca ttaccaaaac tcagctggat
     1741 gatctctttc cctgatggct ttaccgggta gaggtttgga acggtaaccg tccactttcc
     1801 tccaggatac tgggttgccc caatccagac ggtacttcca tagggaataa caacagtcga
     1861 tccgtctgca taactttcag cgggagaaga gtcggcatat ccgtcaaaag atacggttag
     1921 tgtattgtca tactccactg ctgttgccgg tacggcaaga aacaggacta ctaaaatgag
     1981 gagaagatat cgatgatcca tagtagtcca tgtgtgattg aattatatta atttcctgat
     2041 tggttcaata atcctgccag acggttaaaa gactcctgca tgcgggagaa tgcatcatcc
     2101 cagttgatat cgttgatagt ggtgtttttg tcccggttgg tcagcggcat gttgaatact
     2161 tcatccataa aggaggtgag attcgactct gcgtcattgg cattcggggt tatcaggccg
     2221 gaactgttga taaacgacat gtttaccccg ccgtctttat ccagaatact ggtactgatg
     2281 ttatcggctt cggcccgggt gacattatcc ttggtaaata ccatcccgaa tatctccagc
     2341 tttacattga acttatcccc gagtgagctg ttgtaaaaca tattttcgta caccggtgtt
     2401 acggtgatcg aacctatgct ctgattgcca agcctgatat cggtggtctg atccccgata
     2461 catcctgcgg aaaatgtgat gagaacaaga actgcaagaa ctcctgcggc aaccggaatt
     2521 ttcatagaaa tgtgtttgtg ccgactggaa ataaatattt gcggatatcc gctgaaaaat
     2581 aaatttaaaa attttttatt tggccataat cacccttcca taagctatta ttttatcacc
     2641 tcacaacccc aacataccgc caaatggcaa tctatgatat cgatcagttt ttagcatcaa
     2701 aacgagcgat acgcacacgt caggtcatca tctggcactt caaagcctat ttcgcatggc
     2761 tctacggatc atcatcagcc aatgatctca ccgcagcatc tattcgatac atcaccgaac
     2821 cccgcaacca ctttgacaca ctccaaaaat actgcacgta catgagggac gccgcagaca
     2881 cactcccgaa ctcacaacac cagcggctaa cctacatcct tcaatacctc cagtggaatg
     2941 aaatcgaatt gaaatctgct caaaccaacc tcttacgcgg catactcccg ccaaacatag
     3001 aacagcacaa cgacacgccg ctcacacggg aaatgatagc cgcaatcctc gcccactgtg
     3061 acacaatcct tcgggcaatc atcctcgtgc aggtatcatc cggcatgcgg atcaacgagg
     3121 tcataggtct gcgtcacggc gacatttcca tagatactca aatcaaagca cacatccctg
     3181 cagcaagaat gaaaagcagc cagccgcaca catatcacat atcgcatgaa gcctggtctg
     3241 cagtagaaga atggctgaaa atccgcgaca ccgtcaccgc atcgggagcc aaacgaacag
     3301 aaaaaaccct cggacgaaaa aaatcatggg atcctgatca gatattcccc atcacgacaa
     3361 caactctgta ccgaaaattc cgcaccgctc taaaaaatgc gggatattat gaaatatgtc
     3421 aggaaacctc aaggttaacc atatcgtttc atgcattccg gcggtatgcc ttcactcgca
     3481 tgtgcgatca catgcccgaa gagatcgcaa attcgatgat tgcccacact accaaccggc
     3541 tgaaccgatc ctatctccgg ccggatctcg accagtatta cgcaaaagta gagccgtatc
     3601 tgaacatact ggcacctgcg gaatatatcg agctcaaagg ctcaaccgcc gaacaggtgt
     3661 ctgatctggc aaaagcagtc gcttcacagg ttgcaaaaaa tcaggaactc gaggaagaat
     3721 tagaagagat acgcacagca ctgggactct acatgcgtgc ggaaaaaccc gatcctgatt
     3781 gttcataaca aaatcattaa ggaatctaat cctaatatta tgtgtaaata cagagaggag
     3841 agagcaatgg tatatactga taaagagata caggatttct tcacattgat ggaaaaacac
     3901 agaattcaaa cgtatgatac gctacctcaa aagcgacttc cgaaagcaaa atacacgtat
     3961 gtattagatt ataaaacccg tgctaaggtg tgataatgcc aggttggggt gatattctcc
     4021 aggaaattga gggtaatttc gctcacgatg tggtgcgaag aaagtatctg aagcagcttg
     4081 cggatcatac tggacgaaat gtcattgcat attattctgg atggttgcag ttctatgatg
     4141 gagaactgta tatctcggat aatgataaga acgggtttat ggaaaccatc cgggggttag
     4201 atcggacaaa gggtttggat ctgctgattc atactccggg aggtgatatt gctgctacgg
     4261 aatcgatagt ggaatatttg caggaaatgt tcaaaggaga cattcgtgtt atcgtccctc
     4321 aattagcaat gtcggcagga acgatgattg catgttctgc aaaagttatc attatgggaa
     4381 aacagtcctc cttaggtcca actgatccgc agattgtcat ccaaacaaat gcaggtccac
     4441 agcaggttgc cgctcagggg ttaatagaag agttttccac tgcagtggat gaatgtaaca
     4501 gagatcccag aaaattacct ttctggaata tcatcatctc ccagtattca ccaactttga
     4561 taggaaactg tcagaaagca attgagtggt catgcgatct cactcggcaa tggcttctta
     4621 attcgatgtt taaagatgat atggctgcat ctgagatagt tgataatatt gtgatatctc
     4681 tctcaaacca ttcggataca aaagagcacg ggagacatct ttctgcaacc acctgtaaaa
     4741 aaattgggct gaaggttcag atgatggaag aggatcaggc attgcaggat gcaatcctcg
     4801 cggtacatca tgcctatatt catactctat ctgcaac
//
LOCUS       DALUAJ010000208         3177 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_40268,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000208 DALUAJ010000000
VERSION     DALUAJ010000208.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40268"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="O0X37_04420"
     CDS             complement(<1..692)
                     /locus_tag="O0X37_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62198.1"
                     /translation="MKSVAEMLELDSLRFLEPDYASLVNNGEPGILICEAEPTPGGAR
                     MLIDEEENPVLFAVRAGDEWVGTSWNFRSPMEQEFALFTAADGDLYQEPRAAIDDAVR
                     AHFSQILREEIAPAREDLPPDRIEKVRDLLHEVWGASAPGLCLDACAGSGIGSMIIRE
                     MGGRPIAYDNDPALLALGLASGRLAPEDTICIDGTVASAYLPDAERGLGIMLGQMYVY
                     TQNLSQPIVEELA"
     gene            complement(689..1714)
                     /locus_tag="O0X37_04425"
     CDS             complement(689..1714)
                     /locus_tag="O0X37_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HJK62199.1"
                     /translation="MAEGKKRVIVAEPIPENVLIEAEEIPAEEVEQIRDVSPSSAAET
                     EELPAEEPQRSEDASAVATMATAELFKKCILPDKTELQERILKTKNDILVGDHCDIGF
                     GLYGNDVVVCEFCNLTGDVVAEGDLRIDNFCEINGTVICNGDAYLGEGVKVHGKLTVG
                     GNLDIGDNVTIDKEFKAFGDISIRNPMPVILYLLLYVMTMLHLDNEEEVEKTVNAIIS
                     EANSEPLVLPPRTTLDLRYFSVSTPMEVGANCRLHGNIKARAIPLKNDVTLFGGIHAT
                     RRVKIGERTAVHGDVIGQNIRIERGADILGDVSGDKVWLHEDALVSGIIRSPGGLTIG
                     RFEGKKE"
     gene            complement(1745..2968)
                     /locus_tag="O0X37_04430"
     CDS             complement(1745..2968)
                     /locus_tag="O0X37_04430"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HJK62200.1"
                     /translation="MTASKILLLIIDGVGDRPCDCLGGKTPLQAANIPNLDELARTGI
                     CGIMDPVAPGVRAGSDTSHLSLLGYPPQKYYTGRGPLEAAGCGIQMEPGMIGFRANYA
                     TLDDAGNVIDRRAGRISDTTEISAAIRDGVDLSKYGVTIEFEPGTGHRAALALKGAGL
                     SAAVSSNDPKATGIPPKRIHPEGEDTPEAKFTAEVCNEFCRQAEEILKNHPVNLARKA
                     DGQNPANVVLIRGAGAMGVYEPFEERHELSGSVVAAAALIAGIGSSVGLRRIPVKPDT
                     PLAEQVSLVKREFATQDFVLFNIKNADEYGHDGKAMEKKEFLEKIDKELRPLMEMPEL
                     LIVVCGDHSTPCTIKEHSADPVPLIIHGDGARIDQVSSYDEISCAAGALCRIRGGDLM
                     PILLDLIDKTHKYGA"
     gene            complement(3036..3110)
                     /locus_tag="O0X37_04435"
     tRNA            complement(3036..3110)
                     /locus_tag="O0X37_04435"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3073..3075),aa:Met,seq:cat)
BASE COUNT          752 a          851 c          803 g          771 t
ORIGIN      
        1 gcaagctcct caacaatcgg ctgagagagg ttctgcgtgt agacatacat ctggccgagc
       61 atgataccaa ggccgcgttc cgcatccggc agatatgcag atgcgacagt tccatcaatg
      121 cagatggtgt cctcaggagc aagacgtccg gatgcaagac ccagtgcgag cagggcagga
      181 tcgttgtcat aggcaatcgg gcgaccaccc atctcgcgga taatcatgga accgatacct
      241 gatcctgcac atgcatcaag acagagaccc ggagcggatg ctccccatac ttcatgcagg
      301 agatcccgaa ccttttcgat tctgtcaggc gggagatctt cccgtgcagg ggcaatttcc
      361 tcacgaagta tctgtgaaaa atgcgcacgc acggcgtcat caattgcggc acgcggctcc
      421 tgatacaggt ctccgtccgc agcagtaaag agcgcgaact cctgttccat cggactgcgg
      481 aagttccatg aagttccgac ccactcatcg cccgcacgga ccgcaaacag cactggattc
      541 tcctcctcat caatcagcat ccgggcaccc cctggcgttg gttcggcctc acagatcaga
      601 ataccaggct cgccgttgtt cacaagtgat gcataatcag gttcaaggaa gcggagagag
      661 tccagttcca gcatctctgc aacagatttc attctttttt accctcaaac cgtccgatgg
      721 tcagaccacc cggagaacgg atgataccac ttaccagtgc atcctcatgc agccagactt
      781 tgtctccgga gacatcacca aggatatcgg caccacgctc gatcctgata ttctgaccaa
      841 taacatcacc gtggaccgcg gtacgttcac cgatcttgac acggcgggtt gcgtggatac
      901 caccaaagag ggtcacatca tttttcaggg gaatagcccg agctttgatg tttccgtgaa
      961 gacgacagtt tgcaccaact tccattgggg tggaaacgga gaagtaacgg agatccagcg
     1021 tggtgcgcgg aggaagaacg agcggttcag agtttgcttc tgatatgatg gcattgacag
     1081 tcttctccac ctcttcttca ttgtcaaggt gaagcatcgt catgacatag aggagaagat
     1141 agaggatgac aggcatcggg tttctgatac tgatgtcacc gaacgctttg aactctttat
     1201 cgatggtgac gttgtcaccg atgtcaaggt ttccgccgac ggtcagcttc ccgtgcactt
     1261 tgactccctc tccgagatag gcgtcaccgt tgcagatgac agttccattg atttcgcaga
     1321 agttgtcaat gcgaagatcg ccttcagcaa caacatctcc ggtcagattg cagaactcac
     1381 agacgaccac atcgtttccg tacagcccaa acccgatgtc gcagtgatca ccgaccagaa
     1441 tatcattttt tgtcttgaga atgcgttcct gcagttcggt tttgtccggc agaatacatt
     1501 ttttaaagag ttcggcggtt gccatagtgg caacggcaga ggcatcctcg ctgcgctgtg
     1561 gttcttcggc gggaagttcc tctgtttctg ctgcggaaga cggagaaaca tcacgtatct
     1621 gttccacctc ttctgcaggg atctcctccg cttcaatcaa gacattctca ggtatcggct
     1681 ctgcaacgat gacccgtttt tttccctctg ccatactcca ccctgtgtat atatctaata
     1741 atcgttatgc gccgtactta tgggttttgt cgatgaggtc taaaaggatc ggcatcagat
     1801 caccgccgcg aatacgacaa agtgcaccag cggcacagga tatctcatca taagaggaga
     1861 cctgatcgat ccgtgcccca tctccgtgaa tgatcagcgg aacgggatca gcactgtgct
     1921 ctttgatggt acacggggtt gagtgatcac cacagacaac aatcagcaac tccggcatct
     1981 ccatgagggg gcggagctcc ttatcgatct tttccagaaa ctcctttttc tccattgctt
     2041 ttccgtcatg tccatactcg tcggcatttt tgatgttgaa gagtacaaag tcctgtgttg
     2101 caaattcacg cttgactagc gaaacctgtt ccgcaagcgg ggtatcaggt tttaccggaa
     2161 tacggcggag accaactgaa ctgccgattc ctgcaatgag ggctgctgcg gctaccacac
     2221 ttccggagag ttcgtggcgc tcctcaaacg gttcgtacac tcccatcgct ccggcaccac
     2281 gaatcaggac aacgtttgcc gggttttgcc catccgcttt gcgtgcaagg ttgaccggat
     2341 gattcttgag gatctcctcg gcctgacggc aaaattcgtt acagacttcg gcggtgaact
     2401 ttgcttcggg tgtatcctcg ccttccgggt gaatacgttt gggaggaata cctgttgctt
     2461 tgggatcatt gctggaaact gcagcagata gccctgcccc tttcagggca agagcggcac
     2521 ggtgcccggt tcccggctcg aactcaatgg tgacaccgta cttgctgagg tcaacaccat
     2581 cacggattgc ggcagatatc tcagtggtgt cagagattct tccggcacgc ctgtcgataa
     2641 cattcccggc atcatcaagg gttgcatagt ttgcacggaa tcctatcatg cccggctcca
     2701 tctgaatacc gcagccagca gcctcaagag gaccgcggcc ggtgtagtac ttctgtggcg
     2761 gatagccaag caggcttaag tgcgaggtat ctgatcctgc acggacaccg ggtgcaaccg
     2821 gatccatgat tccgcagata cccgttcgtg caagttcatc aaggttcggg atgtttgctg
     2881 cctgaagcgg ggttttgccg ccgagacaat cacatggacg gtcacccaca ccgtcaatga
     2941 tgagaaggag aattttagat gctgtcatta cctgatagat tggacgggta agactaagtg
     3001 tattgtgctt ttagaaaaat ctacagtaaa aacagtagcg aggaatggat ttgaaccatt
     3061 ggtctacggg ttatgagccc gtcgggatct cctgactacc ccacctcgct tctttaagag
     3121 gtataatatg tatgacgaat tcacataaat aaattccgat atgtatcttg catatcc
//
LOCUS       DALUAJ010000209         3790 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_40643,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000209 DALUAJ010000000
VERSION     DALUAJ010000209.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40643"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1667
                     /locus_tag="O0X37_04440"
     CDS             <1..1667
                     /locus_tag="O0X37_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62201.1"
                     /translation="TVEEIDIGGGKHIPSVCCPKSSADDMDDYSHFGFILYAWDHDSN
                     KVEVIPYKWVKDPVEDGHGMILPSTDFRVRNRSGDINNEYTFTMNMPHSKSSLEKHEY
                     ISLSSDGKARIEDDDTVRGYNIKSGNWSEIKVPGTRNIAVIRKINEKICDGKNGKILV
                     VIGDVMLDHYMVCKATEIRQSQRHDMDHDYSLDDSGYWRNEAFDSKLMGGAANVAATC
                     NQVIGTVKLFGITGNDGEGMDLFHTAINDLGEDNVCFTQIQNRYYPTITKIYHTIRYK
                     SETSSEIGIRVNREATESVGSEIVSLAYKKGELNYLLETFKNSIDGADGVLFKDHEKG
                     FLSNDKVFEEIVSIVNERLKTEKPFIVIVDPKYKWDRFKKLTRIDAILPNVKEMTAAL
                     YPDEVDKWKEREQNHSLADEDWDKMSMSQQWWNETGKVKCIAITEDNKGASLYLPAIN
                     SDHCNVVKNIYSISPMIIQKNLPKGAEIGCGDVFDAYFVSCLLNKESVLSSTNLSAEE
                     FYQYALKIANYAAGKKLTGEGSRALSFKSYKKNMFPPTHNEREKAI"
     gene            complement(1817..3121)
                     /locus_tag="O0X37_04445"
     CDS             complement(1817..3121)
                     /locus_tag="O0X37_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HJK62202.1"
                     /translation="MESTVFETFESNVRSYCRKYPVVFSQAKGSVLIDQDGREYIDFL
                     CGAGSCNYGHNNDYIKSKVIEYLMGDGLIHGLDMYSVAKGEFIKCLEEKVLIPRGYDY
                     KVLFPGPTGTNAVEVALKIARKVKGRSNVLALMGAFHGMSLGSLSLTTERSARDACGV
                     ARGNVTHVPHPCMMPGFDTIAYIDMLLTDDHSGVDKPAALIVEAVQGGGGINVLSNEW
                     LKEARALCDKHDMLLILDDIQTGCGRTGSFFSFERAGIVPDIVVMAKSIGGIGMPCSI
                     ALVKPEYDVLLPGEHNGTFRGFQLAFVAGKAAIEYMLEHDLEAETMRKGKIVEEYLSS
                     HLPEIDPSLSFRGLGLMWGIDMGAYPCGTTQQIRKVCFEHGLILELSGREDTVVKIMP
                     PLVIEDELLVKGLDILMKVLAEVVKPLAMPPAKQPILSRPAV"
     gene            complement(3548..>3790)
                     /locus_tag="O0X37_04450"
     CDS             complement(3548..>3790)
                     /locus_tag="O0X37_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62203.1"
                     /translation="VFEAKLELRSVPKDKERIPLADGAGRVCASAIIPYPPGIPFVCP
                     GEVLTGEVISYIRDLRAAGEKVIGVNDHGEITVGRQ"
BASE COUNT         1165 a          934 c          823 g          868 t
ORIGIN      
        1 aaacagttga agaaattgat attgggggag gaaaacacat tccatcagta tgttgcccaa
       61 aatcatccgc agatgacatg gatgattact cgcattttgg attcattctg tatgcatggg
      121 atcatgactc caacaaggtc gaggtcattc cttacaaatg ggtaaaagac cctgtggaag
      181 atgggcatgg tatgattctt ccctctactg atttccgcgt tcggaatagg agtggcgata
      241 tcaataacga gtatacattt actatgaaca tgccgcatag taagtctagc ttggaaaaac
      301 atgagtacat ctctctttct agtgacggga aagctagaat cgaggatgac gacacggtac
      361 gtggatataa tataaaatct ggcaactggt ctgagataaa agtgccggga acaaggaaca
      421 tagcagtcat caggaaaatt aacgagaaga tatgtgatgg aaaaaatggt aaaatattag
      481 tcgtgattgg agatgtgatg cttgatcatt acatggtttg caaagcaact gagattagac
      541 aaagtcagag acatgatatg gatcatgatt actcattaga tgatagtggg tattggagga
      601 atgaggcctt tgactcaaaa ctgatgggcg gtgcagcaaa cgtcgcagca acctgtaacc
      661 aagtaatagg gacggtaaaa ctctttggaa ttacaggaaa cgatggcgaa ggtatggatc
      721 tgttccatac tgcaataaat gacctgggag aagataacgt ctgttttaca caaattcaga
      781 accgatatta tcctacaatt actaaaatct accacacaat tcggtacaag tcagaaacat
      841 catctgagat aggaatccgt gtcaacagag aggcaactga gtcagtcgga tcggaaattg
      901 tatctcttgc ttataaaaaa ggagaattaa actatttact ggagacattc aaaaactcaa
      961 ttgatggtgc tgacggggtt ctttttaaag atcatgaaaa agggttcctc tctaacgata
     1021 aggtatttga agaaattgtt tcaattgtca atgagagatt gaagacggaa aaaccattta
     1081 tagtaattgt agatcccaaa tataagtggg atcgatttaa aaaactaacc cggatagatg
     1141 ccatcctccc aaatgtaaaa gaaatgacag cggcactcta cccagatgag gtagacaaat
     1201 ggaaagaaag agaacaaaac cacagtttag ccgatgaaga ctgggataag atgtcaatgt
     1261 ctcagcagtg gtggaatgaa acgggaaaag ttaagtgtat tgcaattact gaggataata
     1321 agggagcaag tctttatcta cccgcaataa acagcgacca ctgcaatgtg gtaaagaata
     1381 tttattcaat atcaccgatg ataatacaaa aaaaccttcc aaaaggtgca gaaattggct
     1441 gtggagatgt atttgatgcc tatttcgtga gctgtctgct aaacaaggag tcagtactct
     1501 cgagtaccaa cttgagtgca gaagagttct atcagtatgc attgaaaata gccaattatg
     1561 ctgcaggaaa aaaactaacc ggtgaaggga gtcgggctct ctcatttaaa tcctataaaa
     1621 agaatatgtt tccccctaca cataatgaac gagagaaagc catataagaa tgcaacctcc
     1681 catttgaaag ataattccca agatacatgg aatatacaca cgatataggt tgcaataaca
     1741 tccaaaaatt agatctagta aagaatataa tttaatcctg ataaaaaaac tgcgggaaaa
     1801 atcccgcaac cctgttttac acagctggtc ttgaaagaat cggctgcttt gcaggcggca
     1861 tcgcaagcgg ctttacaacc tcggcaagga ccttcatcag aatatccaga cccttcacca
     1921 gcagctcatc ctcaatcacc agcggcggca taatcttcac caccgtatcc tcacggcccg
     1981 acagctcaag aatcagacca tgctcgaaac aaaccttccg gatctgctgc gtcgtaccgc
     2041 acggatacgc acccatatcg ataccccaca tcagaccaag accgcggaac gacagcgacg
     2101 gatcaatctc cggcagatgc gaagacaaat actcctccac aatcttacct ttgcgcatcg
     2161 tctcagcctc cagatcatgc tccagcatat actcaattgc cgccttaccc gcaacaaacg
     2221 ccagctggaa cccgcggaac gtaccattgt gctcgcccgg cagaagaaca tcatactcag
     2281 gcttcaccag agcaatcgaa cacggcatac caataccgcc gatcgacttt gccatcacca
     2341 caatatccgg cacaataccc gcacgctcga acgagaagaa cgaacccgtt ctgccgcagc
     2401 cggtctggat atcatcgaga atcagcagca tatcatgctt atcgcacaga gcacgggcct
     2461 ccttaagcca ctcgtttgaa agcacattaa taccgccccc gccctgcacc gcctccacaa
     2521 tcagcgccgc aggcttatca acaccgctgt ggtcatccgt cagcagcata tcaatgtagg
     2581 cgatcgtatc aaagcccggc atcatacacg gatgcggcac atgcgtcacg ttaccgcggg
     2641 caacaccgca ggcatcgcgg gcactgcgtt ccgtcgtcag ggacagcgaa ccaagcgaca
     2701 tcccgtggaa cgcacccatc agtgcaagca cattcgatct gcccttcacc ttccgggcga
     2761 tcttcagtgc cacctcgacc gcattcgtac cggtcggtcc ggggaacagc accttatagt
     2821 cgtagccacg cgggatgaga accttctcct ccagacactt gataaactcg ccctttgcaa
     2881 ccgagtacat atcaagcccg tggatgagac cgtcgcccat cagatactca atcactttgc
     2941 tcttaatata gtcattatta tgaccatagt tacagcttcc tgctccacag aggaaatcga
     3001 tgtattctct tccatcctga tcaatcagca ccgatccttt cgcttgcgaa aagacgaccg
     3061 gatattttct gcagtacgat ctgacgttgc tttcgaatgt ttcgaatacc gtggattcca
     3121 ttaagacttc atttgtgttt cttaaaccca tagaccacac ccccacatta ctaggatagt
     3181 atgaatttac aggcaatctt actccgcaga tgccacaggg acaacaccgg cgtccctgcc
     3241 ggacgggact acaccagaca cccgtccgtt tttgctgcac cgagattaca cgatcatgtt
     3301 gcccccctca cttcttaaag gtgtccctcc caaaagaaat gattatagcc tgctgtatgg
     3361 actttcggca aaaattcttg gacttcaaaa accccccaca taccctgaat tccccatccc
     3421 tgaccccagg ggggttaaat ggttttcggg ttgatccgat gaccgcagtt ttgggaaaac
     3481 cggacgcgca ttacgggaga aataaaaaac ggattgagcg aaccaaacgt tttcgcaaaa
     3541 aaagaaatta ttggcgtcca accgtgattt cgccgtgatc gttgacgccg attacctttt
     3601 cacctgcggc acggaggtca cggatgtagc tgataacctc cccggtcagc acctcgcccg
     3661 gacagacaaa cggaataccc ggcggatacg ggataatagc cgaggcacag acccgcccgg
     3721 caccgtcggc aagcggaatg cgttccttgt ccttgggaac ggaccggagt tccagtttcg
     3781 cctcaaacac
//
LOCUS       DALUAJ010000210         2023 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_40650,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000210 DALUAJ010000000
VERSION     DALUAJ010000210.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40650"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="O0X37_04455"
     CDS             complement(<1..1158)
                     /locus_tag="O0X37_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HJK62204.1"
                     /translation="MVSLESVFHLKAEKTTVPREVIAGLTTFFAMAYIIIVNPAILSE
                     TGMEWGAVFLATIIAAIAGTLIIGLYANVPFAQAPGMGLNTFFVYTICFVLGFTWQQA
                     LSMVFLCGILNVIMTVTSVRKQLIASIPPSLQAAIGGGIGLFVAYIGLLNVGMIDFSS
                     GVPVLAALNQPVIWVFLIGFAVTLLLLVKQVKGALLIGIAAAAVVGIPLGVTESANVI
                     SISEAWAALPSTFGVIFTDAGLVSLFSDPMQLPLVLVTILAFGLCDMFDTIGTLVGTG
                     RRSGIFSDEDMRMMAENRGMKSKIERSMFSDAIATMIGAVVGTSNVNTYVESATGIAA
                     GGRTGLTSVVVACCLAASAFFASLVSAVPLAATSPILVIVGILMAASFADVA"
     gene            complement(1274..1936)
                     /locus_tag="O0X37_04460"
     CDS             complement(1274..1936)
                     /locus_tag="O0X37_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HJK62205.1"
                     /translation="MYIRYVGHSCFSLEGSRKVLIDPMPEAAASVDADLVLVTHAHGD
                     HLGITVDLKKQTVAIHELANYLSRKGVPAVGMNIGGTYAADRVKVTMVPALHSSSVTE
                     NGVDLYMGSAAGFVVEMDGIVVYHAGDTGLFSDMKLIHDLYHPDVALLPVGGHYTMGP
                     AEAMIAAEWVGAPLVIPMHYNTFPAIAQDLTEFEHAIESTTSMLVDVVAPGDGVEVSP
                     QQ"
BASE COUNT          574 a          644 c          430 g          375 t
ORIGIN      
        1 ggccacatcc gcaaaggatg ccgccattaa aatacccaca atcaccagaa tcggtgacgt
       61 tgccgccaac ggaaccgcac tcaccagact tgcaaaaaac gcactcgccg ccaaacagca
      121 ggcgaccacc acacttgtca gaccggttcg tccgccggca gcgataccgg ttgcactctc
      181 cacatacgta ttcacattcg acgttcccac caccgctccg atcatcgttg cgattgcatc
      241 cgagaacatc gaccgctcga tcttgctttt catcccccgg ttctcggcca tcatccgcat
      301 atcctcatca gaaaagatcc cgcttctccg gccggttccg accagtgtgc cgatggtatc
      361 gaacatatcg cacagtccga acgcgagaat tgtcaccagc accagcggca gctgcatcgg
      421 atccgagaac agcgaaacca gtcctgcatc cgtaaagatg acaccaaacg ttgacggaag
      481 agcagcccac gcctcggaga tgctgatcac atttgccgat tctgttactc cgagcggaat
      541 gcccaccacc gcagccgctg cgattccgat cagaagagca cctttcacct gtttgaccaa
      601 aagcagcagt gtcaccgcaa atccgatcag aaacacccag ataaccggct ggttcagtgc
      661 tgcaagtacc ggaacgcccg aggagaaatc aatcattccg acgttcaaaa gaccgatgta
      721 tgcgacaaac agaccgatac ctccgccgat ggcagcctga agcgagggag gaatggatgc
      781 gatcagctgt tttcgcacac tggtaaccgt catgatcacg ttcaggatac cgcacaaaaa
      841 caccattgaa agagcctgct gccaggtaaa gcccaaaaca aaacagatcg tgtacacaaa
      901 gaatgtgttc agtcccattc ccggcgcctg ggcaaacggg acatttgcat acagaccgat
      961 gatcagtgta cctgcaattg ccgcaataat cgttgccaga aacacggcac cccactccat
     1021 gccggtttcg ctcagaattg cagggttgac aataataatg taggccatcg caaaaaacgt
     1081 cgtaagacct gcgataacct cacggggcac cgttgttttt tcggctttca gatgaaacac
     1141 actttccaga gataccatac agacaacctt cccggaccgc tgctacttca cattcaacgt
     1201 tggcacataa atctgtttgg aaaaatccgg gaacatcccg tcaaaaaaag aacagccgca
     1261 aaaaagcggc atctcactgc tgcggcgaca cctcaacccc gtcgcccggt gcaaccacat
     1321 ccaccagcat cgacgtcgta gactcaatcg catgctcaaa ctccgtcaga tcctgggcaa
     1381 tcgccgggaa cgtattgtaa tgcatcggaa tcaccagcgg ggcaccaacc cactcggccg
     1441 caatcatcgc ctcggcaggc cccatcgtat aatgaccccc aaccggcagc agagcaacat
     1501 ccggatgata cagatcatgg atcagcttca tatccgaaaa cagtcccgta tcacccgcat
     1561 gatacacaac aatcccgtcc atctccacca caaacccggc agcagacccc atatacagat
     1621 ccaccccgtt ctccgtcacc gacgacgaat gcagagccgg aaccatcgtc accttcaccc
     1681 tgtccgccgc ataagttccg ccgatgttca tcccaaccgc aggaacacct ttccgcgaca
     1741 gataatttgc aagctcatga attgccaccg tctgcttttt gagatccacc gtaataccca
     1801 gatgatctcc gtgagcatgc gtcaccagaa ccagatccgc atcaaccgaa gcagcagcct
     1861 ccggcatcgg atcgatcaaa acctttcggg agccctccag tgaaaaacag gaatgtccca
     1921 catagcggat atacataaca gtactccccc tcctacctat aaataaacat gggtcgggga
     1981 aatatcagaa aaaaacggga gggaaatcag ttgatctcaa tac
//
LOCUS       DALUAJ010000211         4535 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_40699,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000211 DALUAJ010000000
VERSION     DALUAJ010000211.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4535)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4535)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4535
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40699"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="O0X37_04465"
     CDS             <1..378
                     /locus_tag="O0X37_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HJK62206.1"
                     /translation="EDDCPCGRGQKIARLFGRSDDMLTVRGINVFPSQIEHVLKNIPE
                     VGDQFMVYIDRLNHLDEMTIEVEINKHLFSGELSDLAKLQNKIGKALHETLTLRANVQ
                     LVEPGSLPRFEGKAKRVVDRRKI"
     gene            381..1682
                     /locus_tag="O0X37_04470"
     CDS             381..1682
                     /locus_tag="O0X37_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HJK62207.1"
                     /translation="MAYYNEKIETLPKAELEKLQYQELKVLVERLYATSVFYHDKMKA
                     AGVLPSDIKSIADIVKLPTMKKTDLRDNYPDKLVCCPKEDLVRYHVSSGTTGKPTVVA
                     YTKNDVELWSEAVARSLVSCGLGRGDVRQVAYGYGLFTGGLGLHYGGEMLGATVIPAS
                     TGNSERQIELIQDLGTTAVACTPSYFIHLIDVAKKMGVDIAKDTKLRTAILGAEPWSE
                     SMRQYIHEQSGVTAHNIFGTSEISGPMFTDCSELNGMHICGDIAYTEILDPETGESLP
                     AGEKGELTMTVLKKEAIPMIRYRIGDITSILEGDCPCGRTSPRIDRLQGRVDDMLVIR
                     GINVFPSAVEHALLRVPELTSHFMLEVTRTGPLDDMLVKVELKPDAMTDSINGLIGLQ
                     ARVERTLRDALNVSAKVELCPPNSLPRFEGKAKRVVDHRVF"
     gene            1683..2120
                     /locus_tag="O0X37_04475"
     CDS             1683..2120
                     /locus_tag="O0X37_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HJK62208.1"
                     /translation="MTRNKYFIKQLSIFSENKSGKLAAIAKVFEECKVNIHAFSIAEA
                     NSFGVVRAIVDKPELAFDAFSKQNYALQYTDVLGIKMNDVPGGLYHVANILGSIGINI
                     EYAYAFRTGTFGTLIVKVSNPEDAVEKILAAGLELVPATDYNF"
     gene            2193..2639
                     /locus_tag="O0X37_04480"
     CDS             2193..2639
                     /locus_tag="O0X37_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HJK62209.1"
                     /translation="MTNEYIIKQLSIFAPNTPGTLAEIAKIFQDNGVNISAFCIAEAK
                     NFGVVRAVVNQPDAAFAAFEKKNYLLKFTDVLAVKMSDVPGGLYEVASKFGEAGLNIE
                     YAYASRSAAVPTLVVYVSNPDIPLETAVERILHSGLSLVPAAEMQV"
     gene            complement(2667..3248)
                     /locus_tag="O0X37_04485"
     CDS             complement(2667..3248)
                     /locus_tag="O0X37_04485"
                     /inference="COORDINATES: protein motif:HMM:NF039705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HVO_0476 family zinc finger protein"
                     /protein_id="HJK62210.1"
                     /translation="MDTDMIILDCPVCGEETDFEILKEPPEAVVRCTECGHTMRVTLK
                     EPRIITVKTIVSYGTESHMGTVELMEGDVCSVGDFLVAEVGDESYGVEVMSIERDNAR
                     RAKLPAEEIDVLWTRLVDKVIIRASLNKGAVTIPLYEQVPGDKVYTVDHITSVGGKQF
                     RITRIKLRKGNIIQRKDKTAQAHEIKRIYGERS"
     gene            3348..3773
                     /locus_tag="O0X37_04490"
     CDS             3348..3773
                     /locus_tag="O0X37_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004039595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF371 domain-containing protein"
                     /protein_id="HJK62211.1"
                     /translation="MEIIETIHCRGHKNVRALHKSTFEITKETDLSPNGDCIIAVGAD
                     RGAADLSPAFRDALSRDGTILTTTLSCGGITATVRSEGCPGITLTHGTDLVWRRSTFL
                     CPRTIAVYSDHTACQLPRELIEKLQNEEEMIVTLRAELP"
     gene            complement(3748..3948)
                     /gene="xseB"
                     /locus_tag="O0X37_04495"
     CDS             complement(3748..3948)
                     /gene="xseB"
                     /locus_tag="O0X37_04495"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HJK62212.1"
                     /translation="MTETPETQTYESMIAELKAIAKQLDDPETPIEEAVRLHQRGLAL
                     IRSCEEFLQTAELTITEVQPEE"
     gene            complement(3932..>4535)
                     /locus_tag="O0X37_04500"
     CDS             complement(3932..>4535)
                     /locus_tag="O0X37_04500"
                     /inference="COORDINATES: protein motif:HMM:NF014640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62213.1"
                     /translation="SEVPVISAVGHETDTTLADYAADIRVPTPSAAAETVVPDRTVLL
                     QQLEEMRRTIRDTVVGRFAAERSTLADLAERVDPSRLSRKLDQMHQQMADFAERITSA
                     LLRRISAEHILCETLRETVAKSARTRIATARLEFLALKEIVVGRDPYKPLELGYALVW
                     KDGTVVRSAKNISTGDHLSLRLSDGDAVTVVENVTYDRDT"
BASE COUNT         1105 a         1097 c         1133 g         1200 t
ORIGIN      
        1 gaggatgact gtccctgcgg tcgcggtcag aagattgccc gtctgtttgg cagaagcgac
       61 gatatgctta cggtgcgcgg cattaacgtt ttcccgtccc agattgagca tgttctcaag
      121 aatatccccg aggttggcga tcagtttatg gtgtacattg atcgcctgaa ccatctggat
      181 gagatgacaa ttgaggttga gattaacaaa catctcttca gcggtgaatt gagtgatctc
      241 gccaagctcc agaacaaaat cggtaaagcc ctgcatgaaa ctcttaccct tcgtgcaaac
      301 gtccagctcg tagagccggg ttctcttccc cggtttgagg gaaaggcaaa gcgggttgta
      361 gatcggagga aaatctaatt atggcatact ataacgaaaa aattgaaaca cttcccaaag
      421 cagagctgga aaaactgcag taccaggaac tgaaagtcct tgttgaacgc ctttatgcaa
      481 catctgtctt ctatcatgac aagatgaaag ctgcaggcgt gctcccgtct gatatcaaat
      541 ccattgcaga tatcgtcaaa cttccaacga tgaaaaagac ggatctgcgt gataactatc
      601 cggacaaact cgtttgctgc ccgaaagagg atcttgttcg ttatcacgtc tcctcaggta
      661 caaccggtaa accaacggtg gttgcctaca cgaaaaatga tgtggagctc tggtctgagg
      721 cggttgcacg ttccctggtt tcctgtggtc ttggtcgtgg cgacgttcgc caagtcgcct
      781 atgggtatgg tctgtttacc ggcggtcttg gcctgcacta cggcggagaa atgctgggtg
      841 caacggtgat ccccgcatca actggaaact ctgagagaca gattgagctc attcaggatc
      901 tgggtactac agcagttgcc tgcactccat cgtacttcat ccatttaatc gatgttgcaa
      961 agaagatggg tgttgacatt gcaaaggata caaagctgag aactgctatt ctcggtgccg
     1021 agccgtggtc cgagtcgatg cggcagtaca ttcacgagca gtcaggagtt acggcccaca
     1081 acatctttgg aacctctgag atctctggac cgatgtttac cgactgcagt gaactgaacg
     1141 gtatgcacat ctgcggtgat atcgcctata cagaaattct ggatcctgag accggcgagt
     1201 cactgcccgc cggagaaaag ggtgagctga caatgacggt tctgaagaaa gaggcaattc
     1261 ccatgattcg ttaccgtatc ggtgatatta cctctattct tgagggagac tgcccctgcg
     1321 ggagaacttc tccacgcatt gatcgtttac agggaagagt tgacgacatg ctggttatcc
     1381 gcgggattaa cgtgttcccg tctgctgtgg agcatgcact tctccgcgtg cctgagttga
     1441 caagtcattt catgctggaa gttacccgta ccggaccgct tgatgatatg ctggtgaagg
     1501 ttgagttaaa accggatgcg atgacagaca gtatcaatgg cctgattggc cttcaggcga
     1561 gagttgagcg gaccctgcgt gacgctctga acgtctccgc aaaagttgag ctgtgtccgc
     1621 caaactctct cccgcggttt gaaggaaaag caaaacgtgt tgtggatcac cgggtgttct
     1681 aaatgacgcg aaataagtac ttcatcaaac agctttcgat cttctccgag aacaagtccg
     1741 ggaaacttgc agcaattgcc aaggtctttg aggagtgtaa ggtcaatatt catgcgttca
     1801 gcattgccga ggcgaacagc ttcggtgtgg tgcgggcaat tgtcgataag ccggaacttg
     1861 cctttgatgc attctcaaaa cagaactatg ctctgcagta cactgatgtt ctgggaatca
     1921 aaatgaatga tgttcccggt ggtctgtatc atgtggcaaa tattttaggc agtattggca
     1981 tcaacatcga gtatgcatat gcattcagaa caggaacgtt tggtaccctg atcgtgaagg
     2041 tatcaaatcc cgaagatgca gtggagaaga tcctcgccgc aggactcgaa cttgttccgg
     2101 ccacagatta taacttctaa tttctttttt tgagtacatc aaaaacttaa tacactatcc
     2161 agtgggagat agacagtagg agtgattgcg tcatgacaaa tgaatatatt atcaaacaac
     2221 tttcgatctt tgctccgaat acgccgggga ccctggccga aattgcaaag atattccagg
     2281 acaacggggt gaacatctca gcgttctgca ttgcagaagc gaagaacttc ggagtggtcc
     2341 gggcggttgt gaatcagccg gatgctgcgt ttgcggcatt tgagaagaaa aactatctgc
     2401 tgaagtttac ggacgttctt gcagtgaaga tgtcggatgt acctggcggc ctgtatgagg
     2461 ttgcatcaaa gttcggtgaa gctggtctga atattgagta tgcatatgca tcacgcagtg
     2521 cggctgttcc gacactggtt gtgtatgttt ccaaccctga tattcctcta gagacagccg
     2581 ttgaaaggat tcttcactca ggtctttccc ttgtgcctgc cgctgagatg caggtataat
     2641 catatttttg aaaaaagtga tagatttcag gaccgctcgc cgtatatccg tttgatctca
     2701 tgtgcttggg cagttttgtc tttccgctga ataatgtttc ctttccgcag tttgatacgg
     2761 gtaatccgga actgttttcc gccaactgag gtgatgtgat ccactgtgta cactttgtct
     2821 cccggtacct gttcatagag cggtatggtt accgcgcctt tgtttaagga agcgcgaatg
     2881 atgactttat ctacgagccg tgtccagaga acatcaatct cttctgcagg aagttttgct
     2941 cttcgggcat tgtctcgttc gatggacata acctcaacac cgtaggactc gtcacccact
     3001 tctgctacaa ggaaatcacc gacggaacat acatctcctt ccataagttc aacagttccc
     3061 atgtgcgact ctgttccgta gctgacaatt gttttgacgg taatgatcct cggttctttc
     3121 agcgtgaccc gcatggtgtg gccgcattcg gtacagcgta caactgcctc aggtggttct
     3181 ttaagaattt cgaaatccgt ctcctcaccg cagacgggac agtcaagaat tatcatatcg
     3241 gtatccattg tgctaatcat gttgcttttt gagatagtta agctactgct gatgtggaca
     3301 tcaagacaat ttatttgtcc cttcacaaca agatcatagc aaaaacaatg gagatcatcg
     3361 aaactatcca ctgccgcggc cataaaaatg tccgggcact gcacaaaagc acttttgaga
     3421 tcaccaaaga aacagatctc tctcccaacg gtgactgcat catcgcggtc ggagccgatc
     3481 gtggtgccgc agatctttct cctgcgttcc gcgacgcact ctcccgcgac ggaacaattc
     3541 tcacgaccac gctatcctgc ggcggtataa cggcgactgt cagatccgag gggtgtcctg
     3601 gtattacgct cacgcacgga acggatctcg tctggagacg cagtactttt ctttgtcccc
     3661 ggaccattgc tgtttacagt gatcataccg catgtcagct tccgcgcgag ctgatcgaga
     3721 agttgcagaa tgaggaagag atgatcgtta ctcttcgggc tgaacttccg tgattgtcag
     3781 ctctgctgtt tgcaagaact cttcacaact gcggatcagt gcaagtcccc gctggtgcag
     3841 gcgcactgcc tcctcgattg gggtttcagg gtcatccaac tgctttgcaa ttgcctttaa
     3901 ctcggcaatc atcgattcat acgtctgagt ttcaggtgtc tctgtcatag gtaacattct
     3961 ccacgacggt gaccgcatct ccatccgaga gtcggagtga caggtggtct ccagtactga
     4021 tattttttgc cgaacggaca actgtcccgt ctttccatac aagggcgtaa ccaagttcga
     4081 gtggtttgta cggatctctt ccaacaacga tctcttttag ggcaagaaac tccagtcgtg
     4141 cggttgcaat cctcgttcgg gcagattttg ccacagtttc tcgaagcgtt tcacacaata
     4201 tatgctcggc agaaattctt cgaaggagag ctgaggtaat tctttccgca aagtctgcca
     4261 tctgctgatg catctgatcc agtttccgcg aaagtcttga aggatcaacc cgctcggcaa
     4321 gatccgcaag cgtacttcgt tctgctgcaa atctcccgac tacagtgtca cggattgtgc
     4381 gccgcatctc ttccagctgt tgcaaaagta ctgtccggtc cgggacaacc gtctccgctg
     4441 ctgccgatgg tgttggaaca cggatatctg ctgcataatc tgcaagtgtc gtatcggttt
     4501 catgaccgac ggcactgatt accggtactt ccgac
//
LOCUS       DALUAJ010000212         2431 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_41308,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000212 DALUAJ010000000
VERSION     DALUAJ010000212.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41308"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            3..239
                     /locus_tag="O0X37_04505"
     CDS             3..239
                     /locus_tag="O0X37_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HJK62214.1"
                     /translation="MIQIAFKDDEFTMMFLHQLPAVNQAKAKAIVSISPSHAKKLLVA
                     LQKSVNEYEEKFGSIAPSAQPDAGSDGFTLRGYS"
     gene            326..397
                     /locus_tag="O0X37_04510"
     tRNA            326..397
                     /locus_tag="O0X37_04510"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:358..360,aa:Thr,seq:cgt)
     gene            complement(521..>2431)
                     /locus_tag="O0X37_04515"
     CDS             complement(521..>2431)
                     /locus_tag="O0X37_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrophobe/amphiphile efflux-3 (HAE3) family
                     transporter"
                     /protein_id="HJK62215.1"
                     /translation="IDNIVAMLPEEITSSIMPNGQLAIILIKLNTGTSSDAQQSILDN
                     LRSMASSADIPPGVTLTFTGNAAMMQDMAGDLGSNMIILLVAAIVLMMIACKVLFNHV
                     RFPLLSIVSVFTGLILTFGMMGLFGLPFSMTTIGAMPILLGIGVDYAIQFHSRLDDER
                     QNNRPLPEAIKLAVSKTGAAVFYAMVASALGFMAMMVSTLPDIRQFGITAILGIVCCY
                     IAALIIIPLAAVITDYQPKPRKASADGGKKETFSEKYNELLRKLAVKVTKFAIPIMLI
                     LCIIGFTGVYLDEEVPVSTDMTSYVPVDMPAIVNINTVTRAMGDTGGFQVYVQGGDLT
                     SPDAIKWMYEWGEHELSLYNTRFTSVSSIATVIVEKNGGVLPTTQVQIDNILNSMSDE
                     DKASYIIDNSQAVISFGMKSLSEAQQRSLVSEVTADVQFFSPPPGIEAVVTGSAYSFV
                     EIMNDIREGKTKMTLLAFVIIFAFLALLYRSAGMAICPLVPIVLIIGWNGAAMYFFGI
                     DYTILTATMGAMTIGVAAEYCIMMVERIYEEMEHHDTLTAVQNGTGKIGTAITVSGCA
                     TMCGFSALMFSNFPIISGFGMVTVIAMGFTLFGAVVAVPATVSIVLKNRKKDRIEGDG
                     DCTHTSMNCRAL"
BASE COUNT          708 a          599 c          523 g          601 t
ORIGIN      
        1 atatgattca gattgccttc aaggatgatg agtttacaat gatgtttctg catcagcttc
       61 cggcagtgaa tcaggcaaaa gccaaagcaa ttgtatccat cagtccgtca cacgcaaaaa
      121 aactgctggt tgcactccag aaaagtgtca atgagtatga agaaaagttt ggatctatcg
      181 caccatcagc gcaaccagat gctggatctg atggattcac tctgcgcgga tactcctaac
      241 cagaccttgg gactgccttt cctaaaggat aggcagataa cttatttatt gggtcacgtg
      301 caatttgata aagcacattt tttgtgccgt tgtggcttag cggtatagcg gctgattcgt
      361 aatcagcagg tcgagggttc aagtccctcc aacggctctc tcattttggc aatacatcca
      421 taaagaaact gtcttgaaca tatttcgaac aaagtgggat actgtatcca aggcccaaaa
      481 caaacggctt acagaactac ccacggagta aaaaaggaga ttataaggcg cggcagttca
      541 ttgaggtgtg agtacaatca ccgtcaccct caattctgtc cttcttgcgg ttctttaaca
      601 caatcgacac agttgcagga acggcaacca ccgcaccaaa cagtgtaaag cccatggcaa
      661 ttaccgtcac cataccaaat ccgctgatga ttgggaagtt ggagaacatc agtgctgaga
      721 atccgcacat tgttgcacaa ccggacacgg taattgcggt accaatcttg ccggtaccgt
      781 tttgtaccgc tgttagtgta tcatgatgct ccatctcctc ataaatacgt tccaccatca
      841 tgatacaata ctcagcagcc acaccgatgg tcatcgcccc cattgttgct gtcaagatcg
      901 tataatcaat accaaagaag tacatcgccg caccgttcca tccaatgatc agcacgatcg
      961 gtaccagtgg acagatagcc atacctgcac tgcgatagag aagtgcaagg aatgcaaaga
     1021 tgattacaaa cgcaagcaat gtcatcttcg tttttccttc gcggatatca ttcatgatct
     1081 caacaaagga gtacgcactg ccggtaacca cagcctcaat acctggcggt ggtgaaaaga
     1141 actgcacatc cgcagtaact tctgagacga gcgaccgctg ctgggcctcc gacaaggatt
     1201 tcatgccaaa gctgattact gcctgggagt tgtcgataat ataggacgct ttgtcctcat
     1261 cactcatgga attcagaata ttatcaatct gaacctgggt agttgggaga acaccaccat
     1321 tcttttccac gattacggtt gcaatactcg aaacgctggt aaaccgtgta ttgtacaacg
     1381 atagctcatg ctcaccccat tcatacatcc actttatcgc atcaggactg gtcagatctc
     1441 ctccctgcac atagacctgg aaacctccgg tatcacccat tgcacgtgtt actgtattga
     1501 tattcacaat ggcaggcata tctaccggca catacgatgt catatcagta ctaacaggaa
     1561 cttcttcatc cagatacacc cctgtaaatc caatgataca gagaataagc atgataggga
     1621 tggcaaactt tgtcaccttc acggcaagtt ttcttaaaag ctcgttgtac ttttcggaga
     1681 atgtttcttt ttttccacca tctgccgaag cttttcgtgg ttttggctga taatctgtta
     1741 taacagctgc cagcggaatg ataatcagcg cagcaatgta acaacagaca ataccaagga
     1801 ttgctgtaat accaaactgg cgaatatccg gcagagttga gaccatcatt gccatgaatc
     1861 caagggcact ggcaaccatt gcgtaaaaca ccgcagctcc cgtcttggat acggcaagtt
     1921 tgattgcttc aggaaggggg cgattgtttt gcctctcgtc atccaaacgg gagtggaact
     1981 gaattgcata gtccacacca atacccagga gaattggcat tgcaccaatc gttgtcattg
     2041 agaacggcag gccaaagagt cccatcatac caaacgtcag aatcagaccg gtgaacacac
     2101 tcacaatcga caacagaggg aaacgtacgt ggttgaacag gactttacag gcaatcatca
     2161 tcaaaacaat tgctgcaacc agcaaaatga tcatgttcga gccaagatca ccggccatat
     2221 cctgcatcat agctgcattt ccggtaaagg tcagcgtgac accaggagga atatcagcag
     2281 atgatgccat gctccttaag ttatcgagaa tactctgctg cgcatcagag gacgttcccg
     2341 tgttcagttt gatcaaaata atcgcaagct gaccattcgg catgatagag gaggtaatct
     2401 cttcgggcag cattgcaacg atgttatcga t
//
LOCUS       DALUAJ010000213         6394 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_41337,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000213 DALUAJ010000000
VERSION     DALUAJ010000213.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41337"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="O0X37_04520"
     CDS             complement(<1..1305)
                     /locus_tag="O0X37_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system ATPase subunit"
                     /protein_id="HJK62216.1"
                     /translation="MFQKKTKRIPVPEYDFVRDGPLVTPEYDPSLTLVEAYWTRPGLA
                     LVVIVLTPANEYQYLVFEPSLTPFEKTVLERLFPGVRDLLIRTDVAMDEGSRTKALYD
                     AMDLYLERFSIDLTPDAVQKIRYYLKRNYLGWGILDPLQQDVEIEDISCDGYSTPIFL
                     YHRRYRNIRTSIRFCTQEELDSMVVRFAQSAGKQISLASPIVDTALADGSRAQLTYGK
                     AVTSCGSSFTIRKFREIPFSPIDLILNNTFTVAEMVYFWMAVEYSQSILFIGGTASGK
                     TTSLNAVAQFIPHLSKVITIEDTREITLSHENWLSSVVTDVANPSDSRGRIEMFDLLK
                     AAMRQRPEYILVGEVRGVEAQTLFQAMNTGHTTFSTLHAGSVDSAIHRLENEPLNVPK
                     AMIASLNIVSSQIRLYREGKQIRRCSEIVEIMGISETKDVVVN"
     gene            1593..2366
                     /locus_tag="O0X37_04525"
     CDS             1593..2366
                     /locus_tag="O0X37_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62217.1"
                     /translation="MPDTGEQRVLHATRIPSFDKVIGGGVIPGSTILVLAEPGSGGRE
                     FIQTSLVNYCSDRVNRVSPPEGVQHPAEIHCISLACSRDVFEQQMEDLWNTKRDSQFQ
                     KMMERVHYTDLGEEIGGDLARLFERINAVPDGSVLFIDSLTVLLPYCTRTPDTWVELV
                     TMMRRVTRSAKKRRTTILFHLTSGILTRGQENELSDTVDVVLNMFWQKNVTVKRQRQM
                     YLLKLDGRMPRIDQRDMVIFNVNITPGTGFEITNMRRVA"
     gene            complement(2405..3697)
                     /locus_tag="O0X37_04530"
     CDS             complement(2405..3697)
                     /locus_tag="O0X37_04530"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HJK62218.1"
                     /translation="MLEFDIDTVLRAGDKLADETEVLVLTYEDLSLDQREMAVAAVSE
                     HAGTSIYIRTVSGGRIGVSATSDPTRWKECLSAAVSSARLAEPVDGWTGLPCPAVLPA
                     GDDPFDPHLAIDPDSAALLLARMNEGATAHPESRVVTAGVSLSKGSSTLANSHGVWYE
                     RRMTGVSLGLDAIADGSTSYEYDVSPFLSRIDPEKMGEQTAFLAAASRNGVAVPTGRQ
                     DVVLSEHVVDSLILELFTESINGRNVLTGKSVFSGKLGETVAAPSFSIADVPMDPKGN
                     SMRRFDTEGTPSSRCSILENGVLSSFLYDCKTAAQAGTASTGHALRGGNGATYISPHC
                     LQVSGTVADVTKEPCLFVREVIGAHTANPLTGEFSVEVANAFLMEDGEFVQPVKEAMI
                     AGNVFEILQQVDGISAETKVFDGAIVPKMRIPSMQVIG"
     gene            complement(3690..5027)
                     /locus_tag="O0X37_04535"
     CDS             complement(3690..5027)
                     /locus_tag="O0X37_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HJK62219.1"
                     /translation="MDPIRYYDIRYVRGNSTTIVVENGTVESAGSNFFGKALVRVLGE
                     HGWGYYCASPFNPDDQTAKQECISRAARAAKLANVPAEIADVPYGTPRSWACSAAEQA
                     LVPIEEKADLLLKMEAGAKIPEICSTSARYSEQYQDVWFEDCNGYSANSSVCRTLFTI
                     SAVAARSGNMQMNYEQEAVVGPLDLSAYLGYGEKCAKCAVDLLDASAVSGGRMPAVLD
                     PAIGGVFAHEAVGHASEGDAVRDGVSVLAGKLGTPIGSPLVTIIDDPSMHGYGYEPFD
                     AEGVACGPTELIKNGVMHAYMHSRETLAAVGEDTGDAGHARAEPGMQPLVRMSNTYIK
                     EGDASYDEIIAECKSGVLLIGSRGGQVDPGRGAFQFNAKYGYRIENGETTKMIRDVSL
                     SGDILSILHNIALCGKERKMSSGMCGKGQSVPVSDGAPHVYLTEAIVGGSGNA"
     gene            complement(5166..>6394)
                     /gene="lonB"
                     /locus_tag="O0X37_04540"
     CDS             complement(5166..>6394)
                     /gene="lonB"
                     /locus_tag="O0X37_04540"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HJK62220.1"
                     /translation="HKGVLFIDEINTLELSSQQSLLTALQEGEFPITGQSDRSSGAMV
                     RTEPVPCRFIMIAAGNLDAMQGMHPALRSRIRGYGYEVYMSETMDDTPENRARLVRFV
                     AQEVQNDGKIPHFDPSAVSEILREARRRSGRKGHLTLKLRDLGGLVRVAGDLARQAGD
                     PATTMKHVVDAKQIARSVEDQISDEYIRRTRDYDLTIVSGTLVGRVNGLAVVGNDAGS
                     ILPITAEVTPSQGAGTVIATGLLKEIAQESIKNVSALIKKFSGTDIRKVDIHVQFIGT
                     YNGVEGDSASVTVATAVISALENIPVRQDVAMTGSLSVRGDVLPIGGVTYKIEAAAKA
                     GIHTVIIPQSNLDDVLIEERYKEMVTIIPVTRIEEVLEYALVPQDRETFEKKLSLIGK
                     HMEIPKMPAVTEKEDA"
BASE COUNT         1668 a         1721 c         1534 g         1471 t
ORIGIN      
        1 atttactacc acatccttcg tctctgaaat ccccataatt tccacaatct cactgcagcg
       61 gcgaatctgc tttccttcac ggtacagccg gatctgggag gaaacaatgt tcagtgatgc
      121 gatcattgct ttcggaacat tcagcggttc attttccagc cggtggatcg cagagtccac
      181 cgaacctgca tgaagcgttg aaaacgtggt gtgtcctgtg ttcattgcct gaaacagtgt
      241 ctgtgcttca accccacgga cctctcccac caggatatac tcaggacgct gacgcatggc
      301 agctttcagc aggtcaaaca tctcgatcct tccacggcta tcggaggggt ttgcaacgtc
      361 agttacaaca cttgacagcc agttttcatg gctcagagtg atctctcgtg tgtcctcaat
      421 ggtgatcact ttggaaagat gcggaataaa ctgcgcaacc gcattcaggg aggttgtctt
      481 tcctgatgct gttccgccga taaagagaat tgactgactg tactccactg ccatccagaa
      541 gtacaccatc tcagcaaccg tgaaggtgtt attcaaaata agatcaatgg gcgaaaacgg
      601 gatttcccga aacttccgga tcgtaaatga tgagccgcag cttgttaccg ccttcccata
      661 cgtcagctgg gcacgtgatc cgtccgctaa ggctgtatcc acgatcggtg atgcaaggga
      721 tatctgtttt cctgccgact gtgcaaaccg aaccaccatt gaatcaagct cttcctgggt
      781 acaaaaccgg atggaggttc tgatattccg gtacctgcgg tgatacaaaa atatcggtgt
      841 actatacccg tcacaggaga tatcctcaat ctccacatcc tgctgcagcg gatcaagaat
      901 gccccatccc agataattcc gtttcagata atagcggatt ttctgcacgg catccggcgt
      961 aagatcaatt gaaaaccgtt ccagatacag atccatcgca tcatacagcg ctttggtcct
     1021 gctcccctca tccattgcca catccgtacg gatcagcaga tcacgaactc ccggaaacaa
     1081 tcgctccaga acagtttttt caaacggggt tagcgagggt tcaaaaacaa gatactgata
     1141 ctcatttgca ggagtaagca caatcacaac cagtgcaaga ccagggcgtg tccagtatgc
     1201 ctccacaagg gtgagggacg gatcatactc gggcgtaaca agcgggccgt cacgcacaaa
     1261 atcatactct ggaactggaa tgcgtttcgt ttttttctga aacatcctct gtttcactcc
     1321 aactcagaag gtgttgagca gaaccggaac cgtacctgtt cccagtggac aaatgttgtc
     1381 gtcagtcaca ggcctcatgc aaacgctcag gcatatatta tcccctaggg catgataata
     1441 cttgccactg aaaatacaaa aattccagaa atcagatgat cagatctctg ttcaattcta
     1501 tttttagaaa atatcaacga gatctgggaa agaaataatc atatgatcac ggaaaattat
     1561 aaattctcaa aaaagggaga taggtgtaat ctatgccgga tacaggagaa cagcgtgtgc
     1621 tacacgcaac ccgcatccca tcctttgaca aagtgattgg aggaggagtc attccgggat
     1681 ccaccattct tgtccttgca gaaccaggtt ccggaggaag ggagtttatc cagacatcac
     1741 tcgtgaacta ctgcagtgat agagtgaaca gagtgtctcc cccggaagga gtgcaacatc
     1801 cggctgagat ccactgtatc tcccttgcat gcagcagaga tgtctttgaa cagcagatgg
     1861 aagatctgtg gaacacaaag agagattccc agttccaaaa aatgatggaa cgtgttcatt
     1921 atactgatct tggggaagag ataggaggtg atcttgccag actatttgag agaataaatg
     1981 cagtgcctga tgggagtgta ctttttattg attctctgac cgttcttctt ccttactgta
     2041 ccagaacacc ggatacctgg gtggaacttg ttaccatgat gcgcagagtg acacggtcag
     2101 ccaaaaaacg aaggacgacc atcctattcc accttacttc gggaatcctc acccgaggac
     2161 aggaaaatga gctcagtgac accgttgacg tggtcctgaa tatgttctgg cagaaaaatg
     2221 ttacagtcaa acgtcaaaga cagatgtacc tactgaagct cgatgggaga atgccgagaa
     2281 ttgatcagcg agacatggtg atcttcaacg tgaacataac cccgggtaca ggatttgaga
     2341 tcacaaacat gaggcgggtg gcataggtac acagcctgat gtacaaaccg caaaaaaagt
     2401 gagattatcc gataacctgc atggacggta tgcgcatctt gggaacaatt gcgccgtcaa
     2461 aaaccttggt ctccgcactg ataccgtcaa cctgctgtaa aatctcaaac acatttcctg
     2521 caatcatcgc ttctttcacc ggctgcacaa actctccgtc ctccatcagg aaagcatttg
     2581 caacttccac cgaaaactct ccggtcagag gatttgccgt gtgagcaccg ataacctcgc
     2641 ggacaaagag acacggttct ttggtcacat cagctactgt gcctgaaacc tgcagacagt
     2701 gcggagagat gtacgttgcc ccgtttccgc cgcgaagtgc atgaccagtg gatgcagtac
     2761 ccgcctgggc ggcagtcttg cagtcgtaga ggaaggagga aagtacaccg ttttcaagaa
     2821 tactgcagcg ggatgaaggt gtaccttcag tatcgaatct ccgcatgctg ttccctttgg
     2881 gatccatcgg gacatcagca atggaaaaag agggggccgc aacggtctca ccaagctttc
     2941 cggaaaaaac agattttccg gtgagaacat ttcttccgtt gatactttcc gtaaacagtt
     3001 ccagaatcag cgagtcaacc acatgttccg acagaacaac atcctgccgt ccggtcggaa
     3061 ccgctacacc attccgggat gcagccgcca aaaatgctgt ttgctcaccc atcttttcgg
     3121 gatcaatgcg ggaaaggaac ggcgagacat catactcgta actggttgag ccatcagcaa
     3181 tcgcatctag tccaagggac actccggtca tacgccgctc ataccagaca ccgtgactgt
     3241 ttgcaagcgt tgaggaacct ttcgaaaggg aaacaccagc ggtaaccaca cgcgattcag
     3301 gatgtgctgt cgcaccttcg ttcatacgtg caagaagcaa cgctgcggag tccggatcga
     3361 ttgcaagatg cggatcaaac ggatcatctc ccgcaggaag gaccgctgga cagggaagac
     3421 cagtccatcc atccacgggt tcggcaagtc ttgccgagga gacggcagca gaaaggcatt
     3481 ccttccaccg ggttggatcc gaggttgcgg acacaccgat tctgccgccg gaaacagttc
     3541 tgatgtaaat ggatgttccc gcgtgctcgg agacagcagc aaccgccatc tcacgctgat
     3601 ccagtgatag atcctcatag gtgagaacaa gtacctcggt ctcatcagcg agtttgtctc
     3661 ctgcacgaag aactgtatct atatcaaact caagcattgc cggaaccccc cacaattgcc
     3721 tcggtaagat acacgtgcgg agcaccgtcc gagacaggaa ccgactgccc tttgccgcac
     3781 atacccgagg acatcttccg ctctttaccg caaagcgcga tgttgtgaag aatcgacaga
     3841 atatctccgg agagggatac gtcacggatc atcttggttg tctctccgtt ctcaatccgg
     3901 tagccgtact tggcattgaa ctggaatgca ccgcgtccgg gatccacctg tccaccacga
     3961 gagccaatga gaagaactcc ggatttgcac tcggcaatga tctcatcata ggaggcatcc
     4021 ccttctttga tgtatgtgtt gctcatacga accagcggct gcatgcccgg ctctgctctg
     4081 gcgtgtccgg cgtcgccggt gtcttcccca actgctgcaa gggtttcccg agagtgcatg
     4141 taggcatgca taacaccgtt tttgatcaac tcggtcggac cgcaggcaac accttccgcg
     4201 tcaaacggtt cgtagccgta accgtgcatg gacggatcat caatgatggt caccaatgga
     4261 gaaccgatcg gtgttccgag ttttcccgca agaacagaaa caccatcacg aacagcgtca
     4321 ccctcgcttg catgaccaac cgcttcatgc gcaaacaccc cgccaatggc aggatccaga
     4381 actgcgggca tcctgccgcc ggacaccgca gatgcatcca gcagatccac tgcgcatttc
     4441 gcacatttct ctccataacc aagatacgcc gaaagatcca gcggaccaac aacggcttcc
     4501 tgttcataat tcatctgcat atttccgctg cgtgcagcaa ccgcggagat ggtaaacaga
     4561 gtgcggcaca ccgacgagtt tgcagaatat ccgttacagt cctcaaacca tacatcctga
     4621 tactgttccg agtaccgtgc agaggtgctg catatctcgg gaattttcgc tcccgcttcc
     4681 atcttcagca gaagatcagc cttttcctca atcggaacaa gcgcctgttc cgcggcagaa
     4741 catgcccagc tgcgaggggt gccataggga acatccgcaa tctctgccgg aacgtttgcg
     4801 agttttgcgg cacgtgcagc acggctgatg cattcctgct ttgcagtttg gtcatccgga
     4861 ttaaagggtg atgcacagta gtacccccag ccgtgttctc ccagaacacg gacaagtgct
     4921 tttccgaaaa aatttgaacc ggctgactcc acagtaccat tctccaccac aatcgtcgtg
     4981 gagtttccgc ggacataccg gatatcatag tagcgtatcg gatccattac tgactattgc
     5041 attggcgaga gaaggataac tgattttgga aaggatgaga gtaacgtaaa agctgggttt
     5101 cacgagtaaa tcagaaagag ccaggaacga gtatgtaact atacaaaaaa agcaagaaaa
     5161 gaagattatg catcttcctt ttcggtgacg gcaggcatct tcggaatttc catgtgcttt
     5221 ccgataaggc tgagtttctt ttcaaacgtt tcccggtcct gcggaacaag cgcgtactcg
     5281 agaacctcct cgattctcgt aaccggaatg atcgtaacca tctctttgta ccgctcctca
     5341 atcagcacat catccagatt tgactgcggg ataatgactg tgtggatacc ggcttttgct
     5401 gcagcctcaa tcttataggt tacacctccg atcggaagca catcgccacg aacagaaagt
     5461 gaacccgtca tcgccacgtc ctggcggaca ggaatattct caagagcact gattaccgca
     5521 gttgccaccg tgaccgatgc ggagtctcct tcaacaccat tgtaggttcc gatgaactgc
     5581 acgtgaatat ccaccttgcg aatatcggtt ccggagaact tcttgatcaa agcactgaca
     5641 ttcttaatgg actcctgtgc aatctccttc agaagaccgg tcgcaataac agtacctgct
     5701 ccctgcgatg gtgtaacctc tgctgtgatc gggaggattg aacctgcatc gtttccaaca
     5761 accgcaagac cgttcacacg accgacaagc gttccggaaa cgatcgtcag atcataatca
     5821 cgggtcctcc tgatgtactc gtccgagatc tgatcctcaa ctgagcgggc aatctgtttt
     5881 gcatccacca cgtgcttcat cgtggttgcg ggatccccag cctggcgtgc aagatctccc
     5941 gcaacccgaa caagaccgcc aagatcacgg agtttcagcg tgaggtggcc cttgcggccg
     6001 gaccggcggc gtgcttcgcg gagaatttca gagaccgcag acggatcgaa gtgcggaatc
     6061 tttccgtcgt tttgtacctc ctgagcaaca aagcggacaa gacgtgcacg gttttctgga
     6121 gtatcatcca tcgtctcgct catgtacacc tcgtaaccat atccgcggat acggctgcga
     6181 agtgccgggt gcataccctg cattgcatcc aggtttcctg ccgcaatcat aatgaaccgg
     6241 cagggaacgg gctccgttct gaccattgca ccagacgatc tgtcgctctg accggtgatc
     6301 gggaactcac cctcctgcag tgcagtcagc agactctgct gtgaggaaag ctccaatgta
     6361 ttgatctcat cgatgaacag cacacctttg tggg
//
LOCUS       DALUAJ010000214         5307 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_41432,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000214 DALUAJ010000000
VERSION     DALUAJ010000214.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41432"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1134
                     /locus_tag="O0X37_04545"
     CDS             <1..1134
                     /locus_tag="O0X37_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62221.1"
                     /translation="ITILLICFGVGGSVTAAETTSNGQIIVTGYTLDPGVFYPGDEGI
                     LTVTLKNVGSTPVNVGMPVLLGGQFMHLNSKQMVPIGLLGAGGETSVSLQIKPTGSTT
                     AGMAYPYFSVDYTIDGGSSVTNPVRVQIPIEIDNSDLSIQLVEKPNMISQGSMGKYKL
                     RIGNPRAYEITGLTVSISGDGITSRQDGYFLGTITANNYTDIEIDIATAGDTNLIVSS
                     EYRNGMNVHTSSVEIPVYVTGSSSVVISDLVATKYENKYTVTGTVYNPGLRDISSVVV
                     VAGPPAIPEGSYQAALGVIEGNDSGEFELTFSVSGDPTTVPIIVTYQDELNMYHQEQN
                     VVNIIDYDPAPTSEPQPQSTPVLIPAFVAVGLVAFGVVRQTRK"
     gene            1144..2562
                     /locus_tag="O0X37_04550"
     CDS             1144..2562
                     /locus_tag="O0X37_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HJK62222.1"
                     /translation="MNLLIFFQLAVRNLRLNKFRSILAILGITIGIIAIATTGMSGAI
                     LENSQFQSMNEIGESIYLQVNYTYLWNQYDESSNYYGVAGGAMVATSVAGDSGVYIQP
                     KMEGITNKQIRELSKITAPYPVIPYKSTMSSFEIVEDEYSDKERPPKNPNDIWSSYVT
                     IYGIENEYLPSMFVLESGKFPKLRSEVLIGSKFAKKNEVTVGDELIFSVYKGGQQRNV
                     KVKICGIVKNTGEGLVISSDNSVIGSDSWFKSNFETYSMLTKGYESAVVVIKDVNVAP
                     EMQVKIDEYLNEKGSRVDITNSSSATQPVKDIIAASSKEMMSMGAIALLVAAVGIFNV
                     MLMSVTQRTHEIGILRSIGTKKRQVLLMFLCEAGIMSILGSLIGGVLSLIFALFKARD
                     MIAESAITLNPNAAIMQQMGLSVDQVQVSLADVLSYTFSANVLMYIPLGILIGITVGL
                     LSALYPAWRAANMDPIDALNEQ"
     gene            2762..3388
                     /locus_tag="O0X37_04555"
     CDS             2762..3388
                     /locus_tag="O0X37_04555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HJK62223.1"
                     /translation="MIKSERSLVVLTCFFVVSLIVSNIIAAKVVCIGWIEIPAAVIAY
                     PITFLCTDVISEIWGREEARRTVHLGFVVQIFSLILIYIAIYLPPAEYMLEFQSSFAE
                     TLGTSARFVLASLVAYGVAQTLDVHLFHWLRGRFEPKWMRNNGSTMVSQLVDTVIFIT
                     IGFWGVVPNLGWMMVSQYVVKWVFALADTPVFYYLTRDHGKQAVKTKE"
     gene            complement(3658..>5307)
                     /locus_tag="O0X37_04560"
     CDS             complement(3658..>5307)
                     /locus_tag="O0X37_04560"
                     /inference="COORDINATES: protein motif:HMM:NF041431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEMAR_RS02690 family S-layer glycoprotein"
                     /protein_id="HJK62224.1"
                     /translation="MVYFVYLDGASASDINPTLQLGQSGMVTTDAGPAVDNGAPLVDN
                     TKQNLQNTWGYFETDASGKRTVQYNTLAYTEDKTYTVKVYATGMKKADVEDAAKVNIH
                     GKDYDSVKVKVEKGAVTISASGDGSYYIGDEITLSGTNTDSDEVYLFITGPNLKTNGE
                     VLKQLPEHVAAPGTNPVSVKTDNTWEYKWDTSGCGLDTGAYTIYATSSPVDKSSLTKT
                     EYATVSVNLKQPFLSAVPSGTVVAKGDKIYIRGTAEGNPSTLALYIFGPNKYVKDSIT
                     VEDDGTYEKKLDISSEWSSNQYFVVIEHPMYNGVIDVDQVYYNGNTVVPTKEQSTYTT
                     LEIKNFGNQTNVGLQGSFVVEGINKLQGSNAADALTKMIDSANIDDIYTKLTFTVAEP
                     WIKIENPGDQGVDTKFTITGTTNLAVDDQILVEVVSSSFNAIDKTSTSSTSGISMTTK
                     VVAGEGTDNVWSVEVDTTNWKLDEYTIKASGIEVDVSTTTNFNVVEKVEVTPTATATG
                     ATPTTTATATATTAPTQTPGFGAFVALAGLGAVALLVLRRN"
BASE COUNT         1312 a         1091 c         1397 g         1507 t
ORIGIN      
        1 atcaccatac tactgatatg ttttggtgta gggggaagtg ttaccgcagc agaaactacc
       61 tccaatggtc agataattgt aactggatac acattagatc ctggagtatt ctacccggga
      121 gatgaaggaa tacttaccgt cacattgaaa aatgtcggat caactccggt caatgttggc
      181 atgccagtac ttctgggtgg ccagttcatg catctcaaca gtaaacagat ggtaccaatc
      241 ggattacttg gtgccggggg agaaacatcc gttagcctgc aaatcaagcc aacaggttcc
      301 acaacagctg gaatggcata tccctacttc tcagttgatt atacgattga tggtggaagt
      361 agtgtaacta acccggtccg tgtacagatc ccaattgaga ttgataattc tgatctgagc
      421 atccagcttg tcgaaaaacc aaatatgatt tcacagggaa gtatgggaaa atacaaactc
      481 cgtatcggta atccacgcgc ctatgagatc accggtctta cggtttctat aagtggtgac
      541 gggatcacca gccgtcagga tggatacttc cttggaacaa ttacagccaa caactacaca
      601 gatatcgaaa tcgacatcgc caccgcgggt gacacaaacc ttattgtatc atctgagtat
      661 cgtaacggca tgaatgttca taccagcagt gttgagatcc ctgtctatgt aaccggaagc
      721 agtagcgttg tcatctctga tcttgttgca acaaagtacg aaaacaagta cactgtgacc
      781 ggaacagtct ataacccggg acttcgtgac atctcatcgg ttgttgttgt tgcaggtcct
      841 cctgcaattc ccgaaggatc ttatcaggca gcccttggtg tcattgaagg taatgacagt
      901 ggtgagtttg aactgacgtt ttctgttagt ggagatccaa caacagttcc aatcatcgta
      961 acatatcagg atgaactgaa catgtatcac caagagcaga atgttgtcaa tatcattgac
     1021 tatgatccag caccaacctc cgaaccgcag ccgcaaagta ctcctgttct gatacctgcc
     1081 tttgtggcag ttggtttggt agcattcggt gttgttcgtc aaaccaggaa ataactgtca
     1141 tctatgaatc tactcatatt ttttcagctt gcagtccgca atctccggct gaataaattc
     1201 agatccattc ttgcgattct tggcattaca atcgggatta tagctattgc aacaacaggt
     1261 atgtctggag caattctgga gaactcacag tttcagtcaa tgaatgagat cggggaatcg
     1321 atctatcttc aggtgaacta cacgtacctt tggaatcagt atgatgagtc cagtaattac
     1381 tatggtgttg ccggcggggc tatggtcgca acatctgttg ctggggatag tggggtgtat
     1441 atccaaccta agatggaggg aattaccaac aaacagatac gggaattaag caagattacc
     1501 gcgccatacc cggttattcc atacaagtcg acgatgtcct cttttgaaat tgtggaggat
     1561 gagtactctg ataaagaacg gcctccgaaa aatcccaatg atatctggag ctcctatgtc
     1621 acaatctatg gtatagagaa tgagtacctt ccttcaatgt ttgtgttgga atccggaaag
     1681 tttccaaagc tcagaagtga ggtgttgatt ggatccaagt ttgccaaaaa gaatgaggta
     1741 actgtaggag acgagctaat tttctcagtg tacaaagggg gccagcagag aaatgtgaag
     1801 gtgaaaattt gtggtattgt gaagaacaca ggtgaagggc tggtgatatc gtctgataac
     1861 agtgttattg gcagtgacag ctggtttaaa tccaatttcg agacctactc gatgctgaca
     1921 aagggttatg aatcggctgt tgtggtgata aaggatgtta atgttgcacc tgagatgcag
     1981 gtgaagatag acgaatattt gaacgaaaaa ggcagtcgcg tagatattac gaattcaagt
     2041 tctgccacac agcctgtcaa agacataatc gccgcatctt cgaaggagat gatgtcaatg
     2101 ggtgccattg ccttgttagt ggcagcagtt gggattttca atgtgatgct gatgtcggtt
     2161 actcagcgaa cgcatgaaat cgggattctg cggagtattg gtaccaaaaa gcggcaggtt
     2221 ctgctcatgt tcctctgtga ggcaggtatt atgtcaattc ttgggtctct tatcggcggt
     2281 gttctaagtc tgatctttgc attgttcaag gcacgtgata tgattgcgga gagtgcaata
     2341 accctgaacc caaatgcggc cataatgcag cagatgggac tttccgtgga tcaggttcag
     2401 gttagtcttg ctgatgtgtt gagttataca ttctcagcga atgtactgat gtatattccc
     2461 ttgggaattc tcataggtat aacagttggt ttgttgtcag ctttgtaccc ggcctggcgt
     2521 gctgcgaata tggatccgat agatgcactg aatgaacaat aacccttcta ttttttaata
     2581 aacattgagt gttgacactt tatgcaagta taatctcaac gtacacagtt ggaggcactg
     2641 ggcaggggat ctggggtacg atatggtatt tggagatgag ataactgggt acatgccatt
     2701 tgtggaaagt agagatatac tttcagcacc aacataatgg aagttttgga tggtgagatg
     2761 attgattaag agcgagcggt ctcttgtggt gctgacctgt ttttttgtgg tgagtctgat
     2821 cgtttccaat attatcgcgg caaaggttgt ctgtattggc tggattgaga ttccggcggc
     2881 agtgatcgcc tatccgatca catttttgtg tacagatgtt atcagtgaga tctggggaag
     2941 agaggaggca cgccggacgg tgcatctggg ttttgtagta cagatctttt ctctgattct
     3001 gatctatatt gcaatttatc tgccgccggc agagtacatg cttgagttcc agagttcgtt
     3061 tgcggagacg cttggtacga gcgcccggtt tgttcttgcg tctctggttg cctatggtgt
     3121 tgcgcagacc ctggatgtac atctgttcca ctggctgcgg ggacggtttg agccgaagtg
     3181 gatgcggaac aacggaagta cgatggtgag ccagctggtt gatacggtga tctttattac
     3241 catcgggttc tggggggttg tgccgaatct gggatggatg atggtgagtc agtatgtggt
     3301 gaagtgggtg tttgctcttg cagatacacc ggtgttctat tatctgacgc gggatcatgg
     3361 aaagcaggct gtaaagacaa aagagtaatt tttgcccagc tattttttct actcatcatt
     3421 ttggagggag aactggtatc tcctatcttc tgtttttgat ggattggctc tgggcaggac
     3481 tattgtcttg ggcaactgta cggagaataa tttgtatcac acaccgattg ttttcgaatc
     3541 ataacatcgg tgtttgagca tttgtaaaaa acagcttctg atttttctca aaaagagatt
     3601 ggtatctctc aggatttctc ctgaggattt atcatctctt tttgtgattc acgctgctta
     3661 gttgcggcgg aggacgagca gtgcgactgc gccaagtcct gcgagtgcaa cgaatgcgcc
     3721 gaagcccgga gtctgggtcg gtgcagtggt tgcggttgca gttgcagtgg tggtcggagt
     3781 tgcaccggtt gcggttgcgg tcggagttac ctcgactttc tcaacaacgt tgaagttggt
     3841 cgtcgtagag acatcgacct cgataccgga tgccttgata gtgtactcgt caagcttcca
     3901 gttggtcgtg tcaacctcga cagaccagac gttgtcagta ccctcgcctg caacgacctt
     3961 ggtggtcata gagataccgg aggtgctaga ggtggaggtc ttgtcaattg cgttgaagga
     4021 agaggagact acctcaacaa ggatctggtc gtcaactgca aggttggtcg taccggtgat
     4081 ggtgaacttg gtatcaacac cctggtctcc cgggttctcg atcttgatcc acggctcagc
     4141 aacggtgaac gtcagcttcg tgtagatatc atcgatgttg gcagagtcaa tcatcttggt
     4201 gagtgcatct gctgcattgg agccctggag cttgttgata ccttcgacaa cgaaggaacc
     4261 ctgcagacca acgttggtct ggttaccaaa gttcttaatc tcaagggttg tgtaagttga
     4321 ctgttctttt gtggggacta cggtgttacc gttgtaatat acctggtcca catcaatgac
     4381 accgttgtac atcgggtgtt cgatgacgac gaagtactgg ttggaagacc actcggatga
     4441 aatgtcaagc ttcttctcgt aagttccatc gtcttcaacg gtgatagagt cttttacgta
     4501 cttgttcgga ccgaagatgt agagagccag agtgctcggg ttaccttctg cggtaccgcg
     4561 gatgtagatc ttgtcaccct ttgcgacaac ggttccggac ggaactgcag acaggaacgg
     4621 ctgtttcagg ttgacggaaa cagttgcata ctcagtctta gtgagggaac tcttgtcaac
     4681 gggagaactg gttgcataga tcgtgtatgc accggtgtca agaccgcatc ctgatgtgtc
     4741 ccacttgtac tcccaggtgt tatcagtctt aactgaaacc gggtttgtac caggtgctgc
     4801 gacatgctca ggtaactgtt tcaggacctc accattggtc ttcaggttcg gaccggtgat
     4861 gaacagatag acttcgtcac tgtcggtgtt ggtaccggaa agcgtgattt catcgccgat
     4921 gtagtaggaa ccgtcaccgg atgcggagat ggttactgct cccttctcga ctttgacttt
     4981 gacactgtcg tagtccttac cgtggatgtt aactttggct gcatcctcaa catctgcctt
     5041 cttcatgccg gttgcataga ctttgacggt gtaggtcttg tcttcggtgt aggctaacgt
     5101 gttgtactgg acagtgcgtt taccggatgc atcggtctcg aagtaacccc aggtattctg
     5161 aaggttttgc ttcgtattat caacaagtgg agcgccatta tcaacagcag gtcctgcatc
     5221 tgtagttacc attccagact gtccgagctg gagtgtcggg ttgatatcgg atgcggatgc
     5281 accgtcaagg tagacgaagt acaccat
//
LOCUS       DALUAJ010000215         2193 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_41890,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000215 DALUAJ010000000
VERSION     DALUAJ010000215.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41890"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="O0X37_04565"
     CDS             complement(<1..378)
                     /locus_tag="O0X37_04565"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HJK62225.1"
                     /translation="MKTVLTIAGSDCSGGAGIQADLKTMAAHGVFGMSVIVSVVAENT
                     ARVIDIQDISPKMIEEQIDAVFEDIVPDAVKIGMLSSPACMKAVAGKLTQYHPRHIVI
                     DPVMFAKNGDPLMEPASVGTLIEY"
     gene            681..1937
                     /locus_tag="O0X37_04570"
     CDS             681..1937
                     /locus_tag="O0X37_04570"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HJK62226.1"
                     /translation="MKVVFPTCCGVDVHKSFLVATIIKTPQDSLQPSYQKKRFSTFNS
                     DLNHFADWLHENDCLDVCMESTGKYWVPVFNILEKRGICVVIANPKWVKAVKGNKDDT
                     KDSKWIGDLFRIGLVKSSFIPDKDIRILRELTRYRYKLTSMRSSEKNRFQNAFTVCNV
                     ALDSVVSDMFGKSATAITDYLTSGEDFDAQHCISLLQRSLKKKADDVLQSIEGYEITA
                     EQKTRIGIIRDHLHYIDTLIERVDICINTMIDKYEGYIVLLRTIPGVDRSSAITILSE
                     IGVDMTQFGSSKRLCCWAGLTPGNNESAGKKKSVRISRAGIYLKPALVQVAHAAVKDK
                     LSPYYSLKYERIAKRRGKKRAIIAIARMILTAVFVMLSNLEEWNPVDLYRADMPEHLK
                     EKQLAKAVKQAVRFLETQGLTVLDSA"
BASE COUNT          550 a          559 c          538 g          546 t
ORIGIN      
        1 atattcgatc agtgtgccaa ccgaggcggg ttccatcagc gggtcgccgt ttttggcgaa
       61 catgaccgga tcaatcacaa tatgccgtgg gtgatactgc gtcagcttgc ccgcgaccgc
      121 tttcatacat gcgggggagg acagcatccc gatcttgacc gcgtcgggta cgatatcctc
      181 gaacactgcg tcgatctgtt cctcgatcat cttcgggctg atatcctgaa tgtcgatgac
      241 gcgcgcagtg ttctccgcca caaccgacac gatcacgctc atgccgaaca cgccgtgcgc
      301 ggccatggtc ttcaggtcgg cctgtatgcc cgcgccgccc gagcagtccg atccggcgat
      361 agtgagcact gttttcatta aaatcaccat gccgaccgtt acactgtcgg cacaaatagg
      421 gatgaaagtg tgcagacaaa cggtcgcctt tcttataaaa tcagattgag gaggaagcac
      481 actacaacgc acggggaggt gagtcgtaga ctgcttccag agcttaaaac agtattataa
      541 tcagtgttgg aaccacctgt tcaagcacaa ataagtggcg ccgtgagccc gtacactaag
      601 gattgtatta acacctgtta attgcctggt ggttcagtca ctgctttctc ctcacaattt
      661 tgcgatgagg agggtgtagt ttgaaagtcg tatttccaac ctgctgtggg gttgacgttc
      721 acaaatcttt tctggtggca accatcatca agactccaca ggatagtttg cagcccagtt
      781 atcagaagaa acgcttttct acgtttaact ctgacctgaa ccactttgcc gactggctcc
      841 atgagaatga ctgtctggac gtctgcatgg aatccactgg gaagtactgg gttcctgtct
      901 tcaatattct tgagaaacgg gggatctgtg tcgtcattgc caacccgaaa tgggtcaagg
      961 ctgtaaaggg caataaggat gataccaagg actccaaatg gattggcgat ttgtttcgca
     1021 tcggcctggt gaaaagcagc tttatcccag acaaggatat ccgcatcctc cgcgagttga
     1081 ctcgttaccg ctacaaactt acatctatga gaagcagcga gaaaaaccgt tttcagaatg
     1141 ccttcaccgt ctgcaatgtg gctctggatt ccgtcgtgtc cgatatgttt ggcaagtccg
     1201 caaccgctat tacggactac ctcacatccg gcgaggattt tgacgcccag cattgcattt
     1261 ctctattaca gcgttctctc aagaaaaaag cagatgacgt gctccaatcg attgagggat
     1321 atgaaatcac agccgaacag aaaactcgca ttggaatcat cagggatcat ctgcactaca
     1381 ttgacacctt gattgagcgg gtagatatct gtatcaatac catgattgac aaatacgaag
     1441 gttacatcgt acttctccgt acgataccag gcgttgaccg cagcagcgca attaccatct
     1501 tgtctgaaat cggtgttgat atgacacagt tcggttcctc gaaacgcctg tgctgttggg
     1561 ccggtcttac cccgggcaac aacgaatccg cagggaaaaa gaaatctgtt cgcatttcac
     1621 gtgcgggaat atatctcaaa cctgcgctgg tgcaagttgc ccacgcagcc gtgaaggaca
     1681 aactttcccc ttactactcg ctcaaatatg agcgcattgc caaacgccgg ggcaagaaac
     1741 gggccatcat tgcgattgcc aggatgattt taaccgctgt tttcgtcatg ctctctaacc
     1801 tggaagaatg gaatcccgtt gacctttatc gggcagatat gcccgagcat ctgaaagaaa
     1861 agcagcttgc caaggctgta aagcaggctg ttcgtttcct tgaaacgcaa ggcctaactg
     1921 tgcttgattc tgcctgatac cttttctcct ctgtcgactg ccctgaaaac ccaaggttgg
     1981 cagggctact ttcgtgcgct cttttttgtc tgcactggtc ccgctcttac tttcacacta
     2041 aacaactcct ttgctgggat ttgatgacac aaggtcattt taccacgcag tgcgtggcgg
     2101 gtcaaggcgg gatgaccact tctcaagcgt tcggcaaagg atatataaga agaaaaaaga
     2161 agcgaacaga gctggcctgc ggtacggcag gat
//
LOCUS       DALUAJ010000216         3356 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_41907,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000216 DALUAJ010000000
VERSION     DALUAJ010000216.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41907"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            9..1034
                     /locus_tag="O0X37_04575"
     CDS             9..1034
                     /locus_tag="O0X37_04575"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HJK62227.1"
                     /translation="MIKVAVNGYGTIGKRVADAVSAQPDMEIIGVSKTKPGAEAFVAK
                     ERGYPLYIADITKKEAFEKAGIPVAGSVEEMIQKADIVVDATPGGVGVGNKALYEKYG
                     KKAIWQGGEDHEVAGFSFNASCNYKDAIGRQYARVVSCNTTGLCRIINLIDSAFGVKK
                     VRATMVRRGGDPGDIKRGPIDAIVLNPVTIPSHHGPDVQSVLPHINIVTSAMIVPTTL
                     MHMHFLNMELKNPATKEQVIELIKAHSRLGLIEKSTGITSTAQLKEYTSDLLRPRGDL
                     WENGIFKDSISVLDGTDLYLFQAIHQEADVVVETVDCIRAMAGDVTSAAESIAITNKA
                     VGLTAIR"
     gene            1044..2228
                     /locus_tag="O0X37_04580"
     CDS             1044..2228
                     /locus_tag="O0X37_04580"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HJK62228.1"
                     /translation="MDFDDLIISPDGTHHLYEGTPIYEHRFQSVGPFCFPGIAAVCDN
                     TGAYHINLLGEPAYDERYVWAGDFAEGVAPVRNKEGRYLFIDEAGKPIAFETYLYATG
                     FSEGSAVVYHETLGATHITTAGELLYGDWYYDARPFAGGRAYVRDDDGWMFITQDGSA
                     LERTEEPNMPFPLRGTVRYVPPKNPIPETLRSSEWDAAAVLMRHGEREPFIKGQPGST
                     KVLTARGKRQSRAFGGALPKVPIRTYASPMIRCVQTGDQILVGAGVNGETQKSLMLGT
                     PSAYVADDDIVKEFYVVNPVKIMSLRYVAGETLPGHYPVEEGTERMFSFVESTLKDGE
                     ISVCVTHDAWIVPFVSVLTGYDFTDDWPNFLDGCIIMRRGGRYFLWWRGSEYPVIRRG
                     SA"
     gene            2225..2743
                     /locus_tag="O0X37_04585"
     CDS             2225..2743
                     /locus_tag="O0X37_04585"
                     /inference="COORDINATES: protein motif:HMM:NF025716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HJK62229.1"
                     /translation="MKAAKRNLIIDICAGFFCGVVIITGFLQWIFYPHQGSGPGGGFI
                     LSHAQLYNLHFAVSMIALCFVVIHLLLHLTWIRHCARTPGKKSRHLKINIGADVAGTV
                     CGVVSLISGLVLWIVYPLGISGKGHIFFLGQPYARWYDIHFFSAVLFTIFLAVHLILH
                     WKWIKRNCSYML"
     gene            2845..3252
                     /locus_tag="O0X37_04590"
     CDS             2845..3252
                     /locus_tag="O0X37_04590"
                     /inference="COORDINATES: protein motif:HMM:NF027567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HJK62230.1"
                     /translation="MTNEVTEFMQNNPVMYLATVDKAGNPKVRPFMYYLERDGKPYFC
                     TSNKKPMYKEMQEHPKVEITVANPAFAWLRIAATVSFSKDMEIKKASIEASPIVKSIY
                     QTAENPDFEIFTIINGTATIADFSGQPPKTFSL"
BASE COUNT          888 a          833 c          851 g          784 t
ORIGIN      
        1 actgtattat gatcaaggtc gcagtcaacg gctatggaac catcggaaaa cgtgtggccg
       61 atgcagtctc cgcacagccc gacatggaga tcattggtgt ctccaaaaca aaacccggtg
      121 cagaagcgtt cgttgcaaag gaacggggat atccgcttta catagcagac atcaccaaaa
      181 aagaggcctt tgaaaaagcg ggaattcctg ttgctggaag tgttgaggag atgattcaga
      241 aagctgacat cgtcgtcgat gcaacacccg gtggtgtcgg agtcgggaac aaggcactct
      301 acgaaaagta cggcaaaaaa gcgatctggc agggtggcga ggatcatgag gtggccggat
      361 tttccttcaa cgcatcctgt aactacaaag acgcaatcgg cagacagtat gcccgcgttg
      421 tctcctgcaa cacaaccggt ctttgccgca tcatcaatct tatcgactct gcttttggcg
      481 tgaaaaaagt tcgtgcaacc atggtccgcc gcggaggaga tccgggagat atcaaacgcg
      541 gacctatcga tgccatcgtc ttaaatccgg tcacaatccc ttcgcaccac ggccctgatg
      601 tacagagtgt tctcccgcac atcaatatcg ttacctcagc aatgattgtg ccgacaactc
      661 tgatgcacat gcacttcctc aacatggaac tgaaaaatcc cgcaaccaaa gagcaggtga
      721 tcgagcttat caaagcacac tcccgcctcg gtttaattga gaagagtacc ggtatcacga
      781 gtacagccca gctcaaggag tacacgtctg atctccttcg tccacgcgga gacctttggg
      841 aaaacggtat cttcaaggac tcaatctcgg tgcttgacgg aaccgatctc tatctcttcc
      901 aggcaatcca ccaggaagca gacgtcgtgg ttgagacggt ggactgtatc cgggcgatgg
      961 caggcgatgt aacgtctgca gccgaatcga ttgcgatcac caacaaagcg gttggtctta
     1021 ctgcaatccg ctaatctttt tttatggact ttgacgatct gatcattagc ccggacggaa
     1081 cacatcacct ttacgagggg acaccgatct acgagcaccg ttttcagagc gtcggcccgt
     1141 tctgttttcc cgggattgct gcggtctgcg ataacacggg agcctatcac ataaacctcc
     1201 tcggtgaacc tgcatacgat gaacggtacg tatgggcagg agactttgcc gagggtgttg
     1261 ctcccgttcg caacaaagaa gggagatacc tgttcattga tgaagcagga aaaccgatcg
     1321 cctttgagac gtatctgtat gcgaccggat tttcggaagg atcagcggtg gtctaccatg
     1381 agacgctcgg tgcaacccac atcactaccg caggagaact gttgtatgga gactggtact
     1441 acgatgcccg accgtttgca ggagggaggg catatgtccg cgacgatgat ggatggatgt
     1501 tcatcacgca ggacggttcg gctctggaac gaacagaaga gccgaacatg ccgtttcctc
     1561 tgcggggaac ggtacggtat gttccgccga aaaatcctat tccggaaaca ttgcggagtt
     1621 cagagtggga tgcggcagct gttctgatgc ggcacggaga acgcgaaccg ttcatcaaag
     1681 gccagccggg atccacaaaa gtccttacgg cccggggaaa acggcagtcg cgtgcttttg
     1741 gtggagcact tcccaaggta cctatccgga catatgccag tccgatgata cggtgcgtgc
     1801 agaccggaga tcagattctg gtcggggcag gtgtcaacgg agagacgcaa aaaagtctga
     1861 tgcttggaac acccagtgcc tacgtcgcag atgatgacat tgtcaaggag ttctacgtag
     1921 taaatccggt gaagatcatg agcctccgtt acgttgccgg tgaaaccctc cccggtcatt
     1981 atcctgttga agagggaacg gaacggatgt tttcttttgt ggaaagtacc cttaaagacg
     2041 gggaaatatc cgtgtgtgtt acccacgatg catggattgt gccgttcgtc tctgtcttaa
     2101 ccggttacga cttcaccgat gactggccga actttctgga cggctgcatc atcatgcgcc
     2161 gcggagggag atatttcctc tggtggcggg gcagcgagta tccagtgatc aggcgaggtt
     2221 ctgcatgaaa gctgcaaaac gcaatcttat cattgatatt tgtgcagggt tcttctgtgg
     2281 agttgtaatc ataaccggat ttcttcaatg gatcttctat ccgcaccagg ggtccggacc
     2341 gggcggaggg ttcatcctct cccacgcaca gctgtataat cttcattttg cggtaagtat
     2401 gatcgcgctg tgtttcgtgg tcatccatct gctgctgcac cttacatgga tccgacactg
     2461 tgccagaact cccggaaaga aaagccgaca cctgaaaata aacatcggag cagatgttgc
     2521 aggaacagtc tgcggtgttg tatccctgat aagcggactg gtactctgga tcgtttatcc
     2581 gcttggaata tctggaaaag gacatatctt tttcctcggt cagccgtatg ccaggtggta
     2641 tgacatccat ttcttttcgg cggttctctt tacgatcttt ctcgcggtac atctgatact
     2701 gcactggaaa tggataaaaa gaaactgttc atacatgctc tgattcccat tgttatagta
     2761 atgcactaaa aggttattat tataacctat aacgatcaat agaggaggag agaattccat
     2821 caggaatctc aggagaaaaa tatcatgaca aacgaagtaa ccgagttcat gcagaacaat
     2881 ccggtcatgt acctggcaac tgttgacaag gccggaaatc cgaaggtccg cccatttatg
     2941 tactatctag agcgtgacgg aaaaccgtac ttctgtacct ccaacaaaaa accgatgtac
     3001 aaagagatgc aggaacatcc gaaagttgag atcacagttg caaacccagc gtttgcctgg
     3061 ctcagaatcg ccgcaactgt ttccttctca aaagacatgg agatcaaaaa agcgagcatc
     3121 gaagcaagtc cgattgtaaa aagcatctat cagactgcag agaacccaga ctttgagatc
     3181 ttcacgataa tcaacggcac cgcaaccatc gccgacttct caggtcagcc gccaaagacc
     3241 ttttctctct aacctctctt ttttgaacta taccttcatc tgatccaaaa gagaactatt
     3301 ttactgcaga ccaatggaag aattcctcgc aacagaactt gccgcactca ggaaag
//
LOCUS       DALUAJ010000217         3177 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_42034,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000217 DALUAJ010000000
VERSION     DALUAJ010000217.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42034"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="O0X37_04595"
     CDS             <1..459
                     /locus_tag="O0X37_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62231.1"
                     /translation="DAIVSIAAKFSDAEYLPYDKYTPDGLWYSRDGGIRFRVDGKVAA
                     EMDLLVKTSDGVRVFGEVIINPSGVRGFRSEISAKKKLLADLYGDPVQFLMVLAEVPP
                     SGNVRCVEGSDAYAVIPGGLLSYAQVHPGEVMKRKLSPSVSTKRVLGTMW"
     gene            complement(484..1449)
                     /locus_tag="O0X37_04600"
     CDS             complement(484..1449)
                     /locus_tag="O0X37_04600"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HJK62232.1"
                     /translation="MLNNPTNPMYPATKIDELLDRMSQTGFQGRKLGESYHIWKEMIE
                     TPGVKIILGLSGAMIPAGMQECLIQLVEHRYVDAIVSTGANIFHDICEHLGVRHYKGT
                     HLTNDEELYQHGIDRIYDVFAVEKEFQNVEHYIGDFGTSLGDVRMSSRAFLRQLGEKI
                     AEERPDGRSLLAACAKYDVPVHVPALADSAIGIGLVTAHRRGAKLTIDQIADASELAA
                     FVESAEKTGVIYLGGGTPKNFTQQTEVIPPLEGESYIGSHSYAIQYTTDAPHWGGLSG
                     CTFEEAVSWGKELPDAKKLQCFCDVTIALPLVVSALIAADVHRCP"
     gene            complement(1597..2370)
                     /locus_tag="O0X37_04605"
     CDS             complement(1597..2370)
                     /locus_tag="O0X37_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HJK62233.1"
                     /translation="MNQKLTVKDVMSKPVSIAKSALIPEALDKMLAEGVDPIVVTSDR
                     VVVGTASRRSIAEKMGSKKTGNIPASQIRVASVTREDFPSVYQDQDVDLLVPLLQRYK
                     IVVVWDEEHRLIGQVTTGDLLKVAKPEGAIDTMVEIAPRISRDDRVVQLHRRMVGDGA
                     TRFIVMDGNKAVGIVTETDIAKILVKLKGEIDKHFENALRNVTAADIMSSPLITTPAN
                     TPVSKVVDLMLSKNISTIPIAEGDKVIGLATRKSLIDAL"
     gene            complement(2536..>3177)
                     /locus_tag="O0X37_04610"
     CDS             complement(2536..>3177)
                     /locus_tag="O0X37_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HJK62234.1"
                     /translation="VANRHKGSLLSALNDDIRAIATERVDSLSPSTSIQEAICLLVKS
                     GHGSFPITNPDNTIAGIVTEYDIMKMLAGTESELIVDEIMTRNPKVVSPDVAINCVTK
                     QMVDHDYRRLPVVSNDLLIGMITATDIMGYLGRGRVFTEMKTGSVDEVLNLPVRDLMT
                     GANIRTMSPDEKINDVAREMLEFGIGAFPVIDEGKLIGIVTEFDLVKGLAGNV"
BASE COUNT          695 a          817 c          773 g          892 t
ORIGIN      
        1 gatgcgatcg tttcaatcgc tgcaaagttt tccgatgcgg agtatctccc ctatgacaag
       61 tatacgccag atggtctgtg gtactcccgt gatgggggta tccggttcag agttgacgga
      121 aaagttgctg ccgagatgga tctcctggtg aaaacctccg acggtgtccg agtattcggt
      181 gaggttatca tcaatccctc cggagttcgt ggtttccgaa gtgagatttc tgcaaagaag
      241 aaacttcttg ctgatcttta cggggatcct gttcagtttc tgatggtcct tgctgaagtt
      301 ccgccgtccg gaaatgtacg atgcgttgag ggttcggatg cgtatgcggt tattccggga
      361 ggccttctgt catatgcaca ggtccatccg ggtgaggtca tgaaacgaaa actttcgccg
      421 tccgtcagca cgaaacgtgt tctcggtacg atgtggtaac cggtaaaaaa gaaatgggct
      481 gtttcaggga cagcggtgaa catctgctgc gatgagtgct gagacaacca atgggagtgc
      541 aatggttaca tcacagaagc actgcagttt ttttgcatcc ggcagttctt ttccccagct
      601 gactgcttcc tcaaaggtac agcccgaaag tcctccccaa tgcggtgcat cggtggtgta
      661 ttgaattgcg tatgagtggc tgccgatgta actctcgcct tccagcggcg gaataacttc
      721 ggtctgctgg gtaaagttct tgggcgttcc tcctccgaga tagatgacac cggttttttc
      781 agcactttcc acaaatgcgg caagttcact tgcgtctgct atctgatcga ttgtcagttt
      841 tgcaccgcgt cggtgtgcgg tgacaaggcc gataccgata gcagagtctg caagtgccgg
      901 gacatgcacc gggacatcgt attttgcaca ggcagcaaga agacttcgtc cgtcgggccg
      961 ttcctctgca attttttcac caagctgtct gaggaatgca cggctgctca tgcgcacatc
     1021 cccaagggat gtaccgaaat ccccaatata gtgctcaaca ttctggaact ccttttctac
     1081 ggcgaaaacg tcgtaaattc tgtctattcc gtgttgatac agttcctcgt cattggtaag
     1141 gtgcgttcct ttgtagtgtc tgacaccgag gtgttcacag atgtcgtgga agatgtttgc
     1201 cccggtcgat acgatagcat ccacgtaccg gtgttccacc agctgaataa gacattcctg
     1261 catccctgcc ggaatcattg caccggaaag tccaagaatg atctttactc cgggggtttc
     1321 gatcatctct ttccagatat ggtagctttc accaagtttt cttccctgaa acccggtctg
     1381 gctcatccgg tccagcaact catctatttt tgttgccggg tacatgggat ttgtagggtt
     1441 attcagcata gtagtaaaaa ttggtcggca gagtttatac cggtgccgaa tccggttgac
     1501 ttactcgaaa tagaactgtt catctcatag attactgtgg tgccgccgga ttcggtacat
     1561 aatctgctgg atagtctgtt aaaaaaatga tgatttttag agcgcatcaa tcagcgactt
     1621 gcgggttgca aggccgatga ctttgtcacc ctctgcgatt ggaatggtac tgatgttctt
     1681 tgaaagcatc agatccacta ccttggatac cggcgtgttt gcaggtgtcg taatcagcgg
     1741 gcttgacata atatctgccg ctgttacatt gcgcagtgca ttttcgaagt gcttgtcgat
     1801 ctctcccttg agtttcacga ggatttttgc aatatccgtt tcggtcacaa taccgactgc
     1861 tttgtttcca tccatcacga tgaatctcgt tgccccgtct cctaccatgc ggcggtgaag
     1921 ctggacaaca cggtcatccc gcgagattct tggggcaatc tccaccatag tgtcgatggc
     1981 tccttccggc tttgcaactt taagaagatc gccggtcgtt acctgtccga tcaaccggtg
     2041 ctcttcatcc catacaacaa cgattttgta tctctggagc agcgggacta acaaatccac
     2101 gtcctgatcc tgataaaccg aggggaagtc ctcacgggtc acgcttgcca cacgaatctg
     2161 tgatgcagga atgttgccgg tctttttgct tcccatcttc tccgcaatac tacggcggga
     2221 tgcggtacca acgaccactc tgtcgctcgt cacaacaatt gggtccactc cttcggcaag
     2281 catcttgtca agagcctcag ggattaacgc tgatttggca attgacactg gcttggacat
     2341 tacatccttt acggttaatt tctggttcat tttgctgcct cctgtatttg aattcttttt
     2401 gtattcaaca tagtatgcaa ggaacacttc ctccagcttt ttcctgtgga atctcccctg
     2461 ctgtctggat cattctcagc accggtcaga tcatctgctc tcttttttcc agagccgatc
     2521 ggtgcaatgt ttgtatcaca cgtttcccgc gagtcctttc accagatcaa actcggtcac
     2581 aataccgatc agtttccctt catcaatcac cgggaatgca ccgataccaa actctagcat
     2641 ctcgcgagcc acatcattga tcttttcgtc cggtgacatg gtccgtatgt ttgctcctgt
     2701 catgagatcc cgcaccggca ggttaaggac ttcgtccacg gaccccgtct tcatctcggt
     2761 aaacaccctg ccgcgtccga ggtatcccat aatatcagtt gcggtgatca taccgatcag
     2821 gagatcattg gacacaaccg gcagacggcg gtaatcatga tccaccatct gtttggtgac
     2881 gcagttgatc gccacatccg gcgaaaccac cttcgggttc cgtgtcatga tctcgtcaac
     2941 aatgagttct gactcggttc ccgcgagcat tttcatgata tcatactcgg tcactattcc
     3001 tgcaatggtg ttgtcaggat tggtgatcgg gaaacttccg tgcccgcttt tgaccagaag
     3061 acagattgcc tcctgaatcg aggttgacgg cgaaaggctg tccactctct ctgtagcaat
     3121 tgcccggata tcatcgttta atgcagaaag gagggaaccc ttgtggcggt ttgcaac
//
LOCUS       DALUAJ010000218         6179 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_42296,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000218 DALUAJ010000000
VERSION     DALUAJ010000218.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6179)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6179)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6179
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42296"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="O0X37_04615"
     CDS             complement(<1..736)
                     /locus_tag="O0X37_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin hydroxylase family protein"
                     /protein_id="HJK62235.1"
                     /translation="MDPIQEFQQEKKERIASYAQNSTLNESAREFSEQTHREKYSYNF
                     TWLGRPIIQYPQDLVAMQEIIWEVKPDLIIETGIAHGGSIIFSASMLELLGGENREVI
                     GLDIDIRAHNRREIESHPMFKRITMFEGDSTSPEIAMRVYDIAKKHEKILVCLDSNHT
                     HDHVLKELKLYAPLVTEGSYCVVFDTIIEDLPDDMFSDRPWGHGNNPKTAVREYLKDH
                     PEFIIDKSMEQKLLVTVAPSGYLKRIK"
     gene            complement(759..1616)
                     /locus_tag="O0X37_04620"
     CDS             complement(759..1616)
                     /locus_tag="O0X37_04620"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HJK62236.1"
                     /translation="MKILVTGATGFIGHHVVQWLADHSYTVIATGTSEEKAKLFAWFR
                     SVTFIPCDYYTEERDFYEFFGKPDLLIHLAWKGLPNYMMRFHLEENLPAEIRFLQSFA
                     AAGKTKIVVTGTCYEYGTAEGCLSEDMPTNPTTVYGTAKDTLRRYLSFLAEEFNIPWN
                     WVRLFYLYGEGQSPKSLLPQLDAAVAHGDSEFPMSGGEQLRDYLPVEKVAEYICRISL
                     QNTVSGVVNCCSGKVISVRRLVEQRVAETGATITLKLGVYGYPAYEGMAFWGDCTKLN
                     NIITMNDEK"
     gene            complement(1613..2842)
                     /locus_tag="O0X37_04625"
     CDS             complement(1613..2842)
                     /locus_tag="O0X37_04625"
                     /inference="COORDINATES: protein motif:HMM:NF020073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HJK62237.1"
                     /translation="MKCRFCGNEITKPVVDLINAPPSNAYLREEQLNEPEIYYPLKIM
                     VCEHCWLVQVDEYASSADIFDDDYLYFSSYSSSWVEHARKYVEMIIPKLSLTEKSRVM
                     EIASNDGYLLQFFVQAKIPCVGVEPSTSTHAAALEKGVESIPEFFGESFAKEMIKARG
                     KQDLIIGNNVLAHVPDINDFVEGLRTALADTGTITMEFPHLLNLLTYHQFDTIYHEHY
                     SYLSLYTVQQIFAAHGLKIYDVETLPTHGGSLRIYACHYSDPRSVTESVGKAVADEIV
                     YGLTKYETYVSFQKQVETIRSAFLAFLLQAKEERKTVVGYGAAAKGNTLLNYCGIKGK
                     QTIAAVADASPHKQGRYLPGSHIPVISPEKIKELQPDYLIIFPWNLRGEIIQQNSYIR
                     EWGGKFVTVIPQLEIIE"
     gene            complement(2839..3399)
                     /gene="rfbC"
                     /locus_tag="O0X37_04630"
     CDS             complement(2839..3399)
                     /gene="rfbC"
                     /locus_tag="O0X37_04630"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HJK62238.1"
                     /translation="MGKLTIHETPMNGLYVVETEPFVDNRGAFARWFCEEELAEVIGE
                     RRIKNVNFSRTAKAGSIRGMHFQRPPHAEMKMVRCIRGKVFDVAIDLRKNSETYLKWY
                     GVELSETNMKLFVIPEGFAHGFQTLEDNVEMLYLHTEFYNKSSEGAIRYNDPSVSIQW
                     PKEITEVSEKDASHPYIDDKLSGVVL"
     gene            complement(3404..4480)
                     /gene="rfbG"
                     /locus_tag="O0X37_04635"
     CDS             complement(3404..4480)
                     /gene="rfbG"
                     /locus_tag="O0X37_04635"
                     /EC_number="4.2.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047733 - CDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HJK62239.1"
                     /translation="MEDLGRMIFDIFCGKQILITGHTGFKGSWLALWLSQLGADVHGY
                     ALPPDTEPNHYRAAHIRELLKSECLADLRDRKKLTAYIHKVQPDVIFHLAAQPLVRRS
                     YAEPVETFDTNVMGSIYVMDAVRALEKPCVVIMITTDKCYQNVNQVWGYRECDPLGGH
                     DPYSASKAAAEIAIASYRDSYFPKEGNVRIASVRAGNVIGGGDWAEDRIIPDAVRAVT
                     SGTELEVRSPQAVRPWQHVLEPLSGYMLLAAKMLSGEKDLTGAWNFGPLPTGAATVRE
                     ITEEFYHIWGKGTVVYDPSLADLHEAAFLRLSSEKAMSILGWQPRWNLAETLSRTAGW
                     YRKYYAGENARKLSLSDIKAYMVE"
     gene            complement(4459..5238)
                     /gene="rfbF"
                     /locus_tag="O0X37_04640"
     CDS             complement(4459..5238)
                     /gene="rfbF"
                     /locus_tag="O0X37_04640"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HJK62240.1"
                     /translation="MKVLILAGGMGTRLAEETSVVPKPMVEIGGHPILWHIMKIYSHY
                     GFNEFIILLGYKGYVIKEYFANYFLHQSDVTFDLGNNSITIHEQHCEPWKVTLIDTGL
                     NTATGGRVLRARKYVGDEPFMLTYGDGVSNIDIQKLIAFHKNHGKLATLTSVQPEGRY
                     GTITSGTDGQISDFVEKPKGDGSWINAGFFVLQPEVFDYIQEGDATIFERGPLETLAR
                     DGELYAYRHEGFWKCMDTLRDKQQLQAMWDEGTTPWKIWEE"
     gene            complement(5440..5952)
                     /locus_tag="O0X37_04645"
     CDS             complement(5440..5952)
                     /locus_tag="O0X37_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62241.1"
                     /translation="MPRQNLYVKDGRSRTTSIRVNPLLYDAVRQQGGNVEDLFETALS
                     AYLHVPSEVLVRLHREEERLEHCYEAIRAGQEAALAKELARLEGERQERSEADAARRE
                     MLAAVYAEMCSFPETGRRRVEQDIAAGGECLYEEKLVMLLMKRLHYPENSVREAVRVI
                     VRNGWRMPEE"
BASE COUNT         1522 a         1627 c         1243 g         1787 t
ORIGIN      
        1 attttattcg ttttaagtat ccgcttggtg cgacggtcac cagcaatttc tgttccatac
       61 tcttatcaat aataaactcc gggtgatctt tcaaatattc gcgtactgcg gtttttggat
      121 tgtttccatg tccccacggt cgatctgaaa acatatcatc tggtaaatcc tcgataatcg
      181 tgtcaaagac aacacaataa cttccctcag ttaccagcgg cgcatacagt tttaattcct
      241 tcaacacatg gtcgtgagta tggtttgaat caagacagac aaggatcttt tcatgctttt
      301 ttgcaatatc ataaacccgc attgcgatct caggggatgt actgtctccc tcaaacattg
      361 ttatccgttt aaacatcggg tgcgattcaa tctctcttct gttgtgtgcc ctgatgtcta
      421 tgtctaagcc gattacctct ctattctctc cacctaatag ttccagcata gatgcagaaa
      481 agataattga tcctccatgg gcaattcctg tctcaataat cagatcgggt ttaacctccc
      541 agataatctc ctgcattgcc actaaatcct gtggatattg gataataggg cgtccaagcc
      601 aggtaaaatt gtatgaatat ttctctctgt gtgtttgttc tgaaaattca cgtgctgact
      661 catttaatgt tgaattctgt gcatatgatg caattcgttc ctttttttct tgttgaaatt
      721 cttgaatggg atccatttta cgtacctctt ctcttcttct atttttcatc gttcatagta
      781 atgatgttat ttaatttagt acaatctccc cagaatgcca tcccctcgta tgccgggtac
      841 ccgtacaccc cgagcttcag tgtgatcgtt gcccccgtct ctgcaacccg ctgttcaaca
      901 agcctacgca ccgagatcac cttgcctgaa cagcagttca ccacaccgga tactgtattc
      961 tggagtgaga tccggcagat gtactcagcg accttttcca ccggcaggta atcccggagt
     1021 tgctcgcctc cggacatcgg aaactcagaa tcaccatggg caactgctgc gtccaactgt
     1081 gggagaagag acttcgggct ttgtccctct ccgtacaaat agaacagccg tacccagttc
     1141 catgggatgt taaactcttc cgcaaggaat gaaagatacc gccgcagtgt atccttcgca
     1201 gttccataca cggttgtcgg gtttgttggc atatcttctg aaaggcagcc ttcggcagtt
     1261 ccgtactcat aacatgtccc ggtaaccacg atctttgttt tccccgctgc ggcaaatgat
     1321 tgcagaaacc gaatctctgc cgggagattt tcctccagat ggaaccgcat catataattc
     1381 ggcaggcctt tccacgcgag atgaatcaga aggtccggtt ttccaaaaaa ttcatagaag
     1441 tctctctcct ctgtgtagta atcacacggg ataaacgtca ccgagcggaa ccatgcaaac
     1501 agctttgctt tctcctctga tgttccggtc gcaatcacgg tgtagctgtg atccgccagc
     1561 cactgcacga catggtgtcc gataaaaccg gtggcacctg tgacaaggat cttcattcta
     1621 tgatctccaa ctgaggaatt accgtcacaa atttacctcc ccattcacga atgtaagaat
     1681 tttgctggat aatttcacct ctgagattcc atggaaaaat tatcaaatag tctggttgta
     1741 attcctttat tttttctgga gaaattaccg gtatatgaga tccgggaaga tatctcccct
     1801 gtttgtgggg ggaagcgtct gcaactgctg caatggtctg cttccctttt ataccacagt
     1861 aatttagtaa tgtgttccct tttgccgcag caccgtatcc gactacggtt tttctctctt
     1921 cttttgcctg taacaggaat gcgagaaatg cagaacggat tgtttccacc tgtttctgga
     1981 aggaaacata ggtttcgtat tttgttagac catagacaat ttcatctgca acagcctttc
     2041 ccacgctctc ggtaactgac cgtgggtctg agtaatggca tgcataaatc cggagagaac
     2101 cgccgtgtgt gggtagtgtc tctacatcat atatctttag accgtgcgca gcaaatattt
     2161 gctgaaccgt atagagagac aaatatgagt aatgttcatg gtaaattgta tcaaactggt
     2221 gataggtgag aagattgagc agatggggga actccattgt tatggtacct gtatctgcca
     2281 gggcagttct gagtccctca acgaagtcat taatatccgg aacgtgggca agcacattgt
     2341 ttccaattat gagatcctgt ttaccacgag ctttaatcat ctcctttgca aaactctctc
     2401 caaagaactc gggaattgat tccactccct tttccaacgc agctgcatgt gtactggttg
     2461 atggttccac ccctacacag ggtatttttg cctgtacaaa aaattggagt agatacccat
     2521 cattagatgc aatttccatt acacgtgact tttcggtaag ggataatttc ggaattatca
     2581 tttccacata ttttcgtgca tgctctaccc atgacgatga ataggaactg aaatagagat
     2641 aatcatcatc aaagatgtct gctgaagatg cgtactcatc aacctgcacg agccagcagt
     2701 gttcacaaac cattattttc agtggatagt aaatctctgg ttcatttagt tgctcttcgc
     2761 gaaggtaagc atttgacggt ggtgcgttga tcaaatctac tacaggcttt gttatctcat
     2821 ttccacaaaa tctgcatttc ataatacaac tcctgataat ttatcgtcta tgtaggggtg
     2881 tgatgcatct ttttcagata cttcggtaat ttctttaggc cattgaattg aaacagaggg
     2941 gtcattatat ctgattgccc cttcgcttga tttattgtaa aattcggtgt gaagataaag
     3001 catttctaca ttgtcttcca atgtctgaaa tccatgtgca aatccttccg gtataacaaa
     3061 taatttcata tttgtttcag atagctcaac tccgtaccat ttgagatatg tctcagaatt
     3121 tttccggaga tcgatggcaa catcaaagac ttttcccctt atacaccgga ccatcttcat
     3181 ctccgcatga ggtggacgct ggaaatgcat gccgcgaatg gaacctgcct tcgctgttcg
     3241 tgagaagttt acatttttga ttctccgttc tccgataact tccgcaagtt cctcctcgca
     3301 gaaccagcgt gcaaaagctc cccggttatc cacaaacggc tccgtctcca ccacatacag
     3361 tccattcatc ggagtttcat gtatcgtcag ttttcccata gttttactcc accatatatg
     3421 ctttaatatc tgagaggctc agttttcgtg cattctctcc ggcatagtat ttccggtacc
     3481 acccggctgt tctcgacagt gtttctgcaa ggttccatcg aggctgccag ccaaggatgc
     3541 tcatcgcttt ctccgatgat agacggagga atgccgcctc atgcaggtct gcaagggatg
     3601 gatcatacac gacagtcccc tttccccaga tgtggtagaa ctcctcggtg atctcacgaa
     3661 cggttgcagc tccggtcggc agtggtccga agttccatgc cccggtcaga tctttttctc
     3721 cggaaagcat ctttgctgca agaagcatgt acccggaaag cggttccagc acatgctgcc
     3781 atggccgtac cgcctgcgga ctcctgactt caagctctgt gccggatgtt actgccctga
     3841 ctgcatcggg aatgatccta tcctctgccc agtcaccgcc tccgatcaca tttccagccc
     3901 ttacagaggc aatccggaca ttaccctcct tcgggaaata ggagtcacga tacgatgcaa
     3961 ttgcaatctc tgccgcagct ttgcttgcac tgtacggatc atgaccaccc aggggatcac
     4021 actcgcggta cccccagacc tgatttacat tctgatagca tttgtcggtc gtgatcatga
     4081 tcacaacgca gggtttctca agcgctctga cagcgtccat tacgtagatg ctgcccatca
     4141 catttgtgtc aaacgtttca accggttctg cataggatct cctgacaagc ggctgtgctg
     4201 cgagatggaa gataacatcc ggctgtactt tgtgtatgta tgctgtcagt ttctttctgt
     4261 ctcggagatc tgcaagacac tcgcttttca gaagttcgcg gatatgtgct gctctgtagt
     4321 ggttcggttc tgtgtctggg gggagagcgt acccgtgcac gtctgcccca agctgtgaaa
     4381 gccataaggc aagccaggat cctttgaaac cggtgtggcc ggtgatgagg atctgtttcc
     4441 cgcaaaaaat atcaaaaatc attcttccca gatcttccac ggagtagttc cttcatccca
     4501 cattgcctga agctgctgtt tatcccgcag ggtatccata catttccaga atccctcatg
     4561 ccggtatgcg tacagttctc cgtcccgtgc cagtgtttca aggggaccgc gctcaaagat
     4621 cgtggcatca ccctcctgaa tatagtcaaa aacctccggc tgcagcacaa agaacccggc
     4681 attgatccag gatccgtctc cttttggttt ctccacgaaa tcagatatct ggccgtcagt
     4741 tccggaagta atagtcccgt atcttccttc cggctgaaca gaggtgagtg tggcaagttt
     4801 tccgtgattt ttgtggaatg caatcagctt ctgaatatca atgttcgaca ccccgtcccc
     4861 ataggtgagc atgaacggtt catctcccac atactttctt gcacgcaaaa cgcgccctcc
     4921 tgtagcagta ttcagccctg tatcaatcag ggtaaccttc cagggttcgc aatgctgctc
     4981 atgtattgtt atggagttgt ttccaagatc aaacgtaaca tcgctctgat gcaggaaata
     5041 attcgcgaag tactctttaa tcacatatcc cttgtatcca aggagaataa taaactcatt
     5101 aaatccgtaa tgtgagtaga tcttcataat gtgccagaga atcggatgac ctccaatctc
     5161 aaccattggt tttggcacaa cactggtctc ctcggcaaga cgggttccca tacccccggc
     5221 aagaatcagg actttcatat tctatctaat gttttttctg aacaaccata tacgttcgga
     5281 taatctgcat ataatctcgg ataacatttt ttcccagttg atatcctcct acagaaggat
     5341 ttcagcattt gttctccttt ccaacagacc accctgtcga taatactatt ctgatcgaac
     5401 gtatctccaa gctctaagct caaaacccct gtaacagcct tactcctccg gcatcctcca
     5461 tccgttccgc acaatcaccc gtaccgcctc ccgtacagag ttctccggat aatgcagccg
     5521 tttcatcagc agcatcacca gcttctcctc gtacagacac tccccgcctg cggcaatatc
     5581 ctgctcaaca cgtcttcgtc ccgtctccgg aaacgaacac atctccgcgt acaccgcagc
     5641 aagcatctcc cgtcgtgcag catccgcctc ggaccgctcc tgtctctcac cttccagccg
     5701 tgcaagctct ttggcaagag ccgcctcctg ccccgcacgg atagcctcat aacagtgctc
     5761 cagacgttct tcctccctat gcagccgtac taacacctcc gacggcacat gcagatacgc
     5821 agacagtgcc gtctcaaaca gatcctcaac attccctccc tgctgccgca ccgcatcata
     5881 cagcagagga ttcacccgga tagacgttgt cctgcttctc ccgtccttca catacagatt
     5941 ctgtcttggc atccctgcac cttttcatta ttttttaaaa gagtatccct cttcttactc
     6001 ttactgtact gtactcataa gtacagtaca tagtatataa taataaacag accatttttt
     6061 ccaaaacgat cacgaacata tctctggccc cctcccacat caaactatct ttccagacaa
     6121 actctcttaa tgatatcata gatatccttg ataaaaagat atatctgaaa gtatggaga
//
LOCUS       DALUAJ010000219         2244 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_42447,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000219 DALUAJ010000000
VERSION     DALUAJ010000219.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42447"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            151..1725
                     /locus_tag="O0X37_04650"
     CDS             151..1725
                     /locus_tag="O0X37_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HJK62242.1"
                     /translation="MAVSTELTILIVIFYLVLILGLGYYGYRKTTKVEDYMVAGRNTH
                     PMVIALSYGATFISTSAIVGFGGTAAQYGMSLMWLTVLCIVVGVIIAFLFMGKRIRRI
                     GKKLGALTYPELLGKMFGSKFLIYASGLIFVAAMPLYTAAVLIGGARFIETTLGVPYM
                     TALLGFAGIVALYVVIGGLIAVMYTDAVQGAIMLAGMGILLVLTYVYLGGVTAANSAL
                     EAMTPLVPENLAAIGMTGWTSMPEFGSPVWMTVITTLVLGVGIGVLAQPQLAVRFMTA
                     KDGKSLNRAVPMGALFIVMMTGVAFTVGPLANVYFMETTGQLALAAAGGNADSVIPLY
                     INSAMPELFVIIFMLTLLSAAMSTLSSLLHTMGTTLGGDMFARLKGKRYSVRANQIGI
                     VVMMLASLAIAVVMPESIIARATAMFMGLCTAALLPAFTHGIVAKRPSLLAAKWSMIA
                     GAGGWLLWTMFAHTAESQALGICNPLFGVNSLVASPWNYLDPLVVGTVLSVIVLLVLI
                     PLDKNRITPEQQRSFV"
     gene            complement(1860..2153)
                     /locus_tag="O0X37_04655"
     CDS             complement(1860..2153)
                     /locus_tag="O0X37_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62243.1"
                     /translation="MLDVLAETAMAVNVRVRCGNFSGLSGLLLLMKNCCHAVCITDEA
                     DLDVLGDQKVLVRPFGRASERSAVRIVFREEMEKDPLMQKLFLVMDAVNSGSN"
BASE COUNT          539 a          480 c          619 g          606 t
ORIGIN      
        1 ctaagacgca aatccccacg aataatggat gctgtagtat gtttggaatt agcgaccagg
       61 gggtgtggat gacgtacgtc atctgtatcg cggcactgat tggctgtatt gtgtatggat
      121 acctgaactg gaacaaggat agtgatgacg atggccgtga gtactgagct tacgattttg
      181 attgttatct tctatctggt gctgattctt ggtcttggtt actacgggta ccggaagacg
      241 acaaaagttg aggattatat ggttgcggga cggaacacac atccgatggt aattgccctg
      301 tcgtatggtg cgacatttat cagtacatcg gcaattgtgg ggtttggcgg aacggcagca
      361 cagtatggta tgagcttgat gtggcttacg gtgctgtgta ttgtagtcgg tgtgataatt
      421 gcattcctgt tcatgggaaa gcgtatccgt cgtattggga agaaactcgg ggcactaaca
      481 tatcctgagc tgctcggtaa gatgtttgga tcaaagtttt tgatttatgc atcaggtctg
      541 atctttgttg cggcgatgcc gctgtatacg gctgcggttc tgatcggcgg agcacgtttt
      601 attgagacaa cgctgggtgt tccgtacatg acggctctgc ttggttttgc aggcattgtt
      661 gcgctgtatg tggtcattgg cggtctgatt gcggtgatgt ataccgatgc tgtgcagggt
      721 gcgattatgc ttgcaggtat gggtattctg ctggttctga catatgtgta tctgggagga
      781 gttacggcgg caaacagtgc acttgaagcg atgacaccgc ttgttccgga gaatcttgcg
      841 gctattggta tgacgggatg gacatcaatg ccggagtttg gatctcctgt gtggatgacg
      901 gtaattacaa cactggtgct tggagtgggg attggcgttc ttgcacagcc acagcttgcg
      961 gtccggttta tgacggcaaa ggacgggaaa tcactgaacc gtgcggtacc gatgggggcg
     1021 ctgtttattg tgatgatgac aggagttgcc tttacggtag ggccgctggc taatgtatat
     1081 tttatggaga ctacaggaca gcttgctctt gcggctgccg gtggaaacgc ggattcagtg
     1141 attccgctgt atattaactc agcgatgccg gaactgtttg tgattatttt catgctgacg
     1201 cttttgtctg ctgcaatgtc aacactgtca tctcttctgc acacgatggg tacgacactc
     1261 ggcggtgata tgtttgcacg actgaagggg aaacgatact cagtgcgggc aaatcagatc
     1321 ggtattgtgg tgatgatgct ggcaagtctt gcgattgcgg tggttatgcc ggagagtatc
     1381 attgcccgtg cgacagcgat gttcatgggt ctgtgtactg cggcactact gcctgcattt
     1441 acacatggta ttgttgcaaa acgaccgtct ctgcttgcag cgaagtggag tatgattgcc
     1501 ggtgcgggag ggtggctgtt gtggacaatg tttgcccata ctgcagagtc acaggcactt
     1561 gggatctgta acccgctgtt tggggtgaat tctctggttg caagtccgtg gaattatttg
     1621 gatcccctgg tggtgggaac agtgctgtcg gtgattgtat tgctggtact gattcctctg
     1681 gataagaaca ggataacacc agaacagcag agaagttttg tgtgagagga ccacaaaagg
     1741 agaaaaacca gacgacataa aaaatcacca ctgtaaactt atcacaaaac aaacaaaaaa
     1801 gaacagaaaa agaggtagca ggtaagacta gctacacact ccttttattt caacaatact
     1861 tagttagacc cactattaac tgcgtccatc accaagaaca gcttctgcat cagaggatct
     1921 ttttccattt cttcgcggaa aacgatcctt accgctgatc tctctgaagc ccggccaaac
     1981 ggacgaacca gtaccttctg atctccaaga acatcaagat ctgcctcatc cgtaatacat
     2041 actgcatgac agcagttctt catcagcagc agcagacctg acaaaccact gaagttccca
     2101 cacctgaccc gaacattgac agccattgcg gtctccgcca aaacatccag caacggatca
     2161 tacacgccag caatcagtac tgttctctca agctcctacc gcggcacggt aaggagagca
     2221 ggaacctcct ctcctgcctc aaaa
//
LOCUS       DALUAJ010000220         2826 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_42530,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000220 DALUAJ010000000
VERSION     DALUAJ010000220.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42530"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="O0X37_04660"
     CDS             complement(<1..246)
                     /locus_tag="O0X37_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase"
                     /protein_id="HJK62244.1"
                     /translation="MIDLPQSVRVAVCQLAPILFDKASTVQKTIEVIDEAARHGAELV
                     VFPESFIPAYPRGLSFGYVVGSRSMEGRYDWKRYYDNS"
     gene            complement(243..644)
                     /locus_tag="O0X37_04665"
     CDS             complement(243..644)
                     /locus_tag="O0X37_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HJK62245.1"
                     /translation="MVLSVQKEDIDIGALITASRTHADGAQLVFVGCVRDDGMDELEI
                     EAFVPVAEKDLADIAAEAKEQFHLNSVDIVHRYGRLALGETIVVIIVGASHRPEAYDG
                     SRYIIERLKERVPIWKQEISDGKKGAWVHGA"
     gene            complement(648..926)
                     /locus_tag="O0X37_04670"
     CDS             complement(648..926)
                     /locus_tag="O0X37_04670"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HJK62246.1"
                     /translation="MSEITILAFASFREKFGEKNTVTISNGATILSVLHSFAESVPAA
                     RDELFDGDSLRGHVILMYNRERIDADDAKEIAVSDGDEIVLYPPVSGG"
     gene            complement(923..1648)
                     /locus_tag="O0X37_04675"
     CDS             complement(923..1648)
                     /locus_tag="O0X37_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HJK62247.1"
                     /translation="MNERYLRQIPVIGDDGQKKLEDATVFLAGCGGLGSPVAFYLAAA
                     GVGHLRIADCDVVEETNLNRQILHRPDRIGMPKAASAKLTLEAFNPDIDVTAFSAFID
                     RNTVRRMIGDADIIVDAMDNFSARYILNEISQTLDIPLVHGGVTGLTGQVTLIIPRKT
                     ACLSCIFPDAKTTTVTPILGAAAGVIGSIQAEETIKYLTGTGDTLAGKLLLWDGAVNR
                     MDVFSVKKSRRCPVCSNICEDKP"
     gene            complement(1645..2283)
                     /locus_tag="O0X37_04680"
     CDS             complement(1645..2283)
                     /locus_tag="O0X37_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012107546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfuA-related McrA-glycine thioamidation protein"
                     /protein_id="HJK62248.1"
                     /translation="MPNAAVFIGPSLPLEEAKQILPDAEYLPPAKRGDLTEAVKAGAK
                     IIVLIDGVFFQDRAVGHREILSALRAGVAVYGSSSMGALRASEMDTLGMVGVGSIYRW
                     YHDGLIIADDEVGLVYDPETHAALSEPMVNIRATFAKARASDIIDDAEEKELLRACKA
                     IYYPDRTYRRVLRDAEISADKKTILAAWIKTNAVDQKRLDAIACLNTVRASL"
     gene            complement(2240..>2826)
                     /locus_tag="O0X37_04685"
     CDS             complement(2240..>2826)
                     /locus_tag="O0X37_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HJK62249.1"
                     /translation="TGAAAEILAKFTAAGVEVTLKDITSDIGIPTIAAVADDITLKDP
                     RLLTIGMGTHTNPEIAVIRALSEVAQSRATQIHGAREDATIAAFREMMGYDRVKRMNA
                     YWFRVEETRDFSEIPGCATSDFRTDILAIIDRLRAAGLDRVLVVDLTDPELGVPVVRV
                     VVPGLECFTIDNERRGKRCLDAERRRLHRSKSSA"
BASE COUNT          731 a          760 c          668 g          667 t
ORIGIN      
        1 ggagttgtca tagtagcgtt tccagtcata tctgccctcc atacttcggc taccgacaac
       61 gtatccaaac gagagaccgc gcggatatgc aggaatgaaa gactcaggaa acaccacaag
      121 ttccgcacca tggcgggctg cctcatctat gacctcaatg gttttctgta ctgttgatgc
      181 tttgtcaaac aggatagggg caagctggca gacagcaaca cgaacagatt gcggaagatc
      241 gatcatgcac cgtgtaccca tgcacctttt tttccgtcag agatttcctg tttccagata
      301 ggaacccgtt ccttcagccg ctcaatgatg taccgtgacc catcataggc ctcaggccgg
      361 tgactcgcac ccacaataat caccacgatc gtctcaccaa gtgcaagacg accataccgg
      421 tgtacaatat ctaccgagtt caggtgaaac tgttcctttg cctcagcagc aatgtcggca
      481 agatcctttt ccgccaccgg tacaaacgct tcaatttcaa gttcgtccat gccatcatca
      541 cgaacacagc cgacaaacac cagttgtgca ccatcggcat gtgttcggga tgcagtaatc
      601 agggcgccaa tatcaatatc ctctttttga acagacagga ccattgttta tcctcccgag
      661 accggcgggt acagcacgat ctcgtcacca tcagagacgg caatttcttt tgcatcatcg
      721 gcatcgattc tttcacggtt gtacatcaga atgacatgcc cgcgaaggct gtcaccatca
      781 aacagttcat cacgggcagc cggaacagac tctgcaaagc tgtgaagaac tgacagaatt
      841 gttgcaccgt tggagatggt aacagtattt ttttcaccga atttttcacg gaagctggca
      901 aaggcaagaa ttgttatttc actcatggtt tatcctcgca gatattggaa cagacaggac
      961 agcgtcggct ctttttcaca gagaaaacat ccattctgtt taccgcacca tcccagagca
     1021 gaagttttcc ggcaagcgta tctcctgtgc cggttaggta tttaatggtc tcctcagcct
     1081 ggattgatcc aatcactccg gcagcggcac ccagaatcgg tgtaactgtt gttgttttcg
     1141 catccgggaa gatgcaggac agacaggcgg ttttccgagg aatgatcaga gttacctgac
     1201 cggtaaggcc agtaacacca ccgtgcacga gcgggatatc aagcgtctga gagatctcgt
     1261 ttaagatgta cctggcagaa aaattatcca ttgcatcaac gatgatgtca gcatccccaa
     1321 tcatacggcg gacagtgttt ctgtcaatga aggcagaaaa tgccgttaca tcaatgtccg
     1381 gattgaaagc ttccagagtt agctttgcgg acgcagcttt cggcataccg attctgtcgg
     1441 gccggtgcag gatctggcgg ttaaggttgg tctcctccac aacatcacag tcagcaatgc
     1501 ggagatgacc aacccctgca gctgcaagat aaaaggcaac aggagatcca agcccaccgc
     1561 atccggcaag aaacactgtt gcgtcctcaa gttttttctg tccgtcatct ccgatcacag
     1621 ggatctgacg gagatagcgc tcattcataa ggatgccctc actgtattca ggcaggcaat
     1681 ggcatcgagc cgtttctgat ccaccgcatt cgttttgatc caggcagcaa gtatcgtttt
     1741 tttatcagca gaaatttctg cgtcacggag aacacgccga taagtgcgat ctggataata
     1801 aatcgcttta caggcgcgaa gaagttcctt ctcttcagca tcatcaataa tatctgatgc
     1861 acgggctttt gcaaatgttg ccctgatatt gaccatgggc tcagaaagtg cggcatgtgt
     1921 ttcaggatca tagacgagac caacttcgtc gtcggcgatg ataagcccgt cgtgatacca
     1981 gcggtaaatg gaaccaacgc caaccatacc gagggtgtcc atctcagacg cccgtaaagc
     2041 ccccatcgaa gaggaaccat acacggcaac acctgcccgc agagcactga gtatctcgcg
     2101 gtgtccaact gcccgatcct ggaagaaaac accgtcaatt aagacgataa tctttgcacc
     2161 tgccttgaca gcctcggtga gatcaccgcg ctttgccgga ggcagatact ctgcatccgg
     2221 caaaatctgt tttgcctcct caagcggaag acttggaccg atgaagacgg cggcgttcgg
     2281 catcaaggca cctctttcct ctgcgttcat tatcaattgt gaaacactca agccctggga
     2341 ccaccacacg tactaccggc acaccaagct ctggatcagt gaggtcaaca accagtacgc
     2401 gatcaagtcc cgctgcacgc agacgatcga taattgccag aatatctgtc cggaagtctg
     2461 acgttgcgca gccaggtatc tcagagaagt cccgggtttc ttccacgcgg aaccagtatg
     2521 cgttcatacg cttaacgcga tcatatccca tcatttcgcg aaatgcagca atggttgcat
     2581 cttctcgtgc cccgtgaatc tgggttgcac ggctttgggc aacctcggat aaagcacgta
     2641 tgacagcaat ttcagggttc gtatgagtac ccatgccaat ggttagaaga cgcggatctt
     2701 tgagggtgat atcatctgca accgcggcaa tggtcggaat accaatgtct gaagtgatgt
     2761 ctttgagggt gacctccacc cctgcagcag taaactttgc aagaatctct gccgcagctc
     2821 cagtaa
//
LOCUS       DALUAJ010000221         3057 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_42836,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000221 DALUAJ010000000
VERSION     DALUAJ010000221.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42836"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(257..1456)
                     /locus_tag="O0X37_04690"
     CDS             complement(257..1456)
                     /locus_tag="O0X37_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK62250.1"
                     /translation="MTDLPCASHRSLAKRIAVLAAGHGAVDFYIPVISAVLPVLLPIF
                     AEQGITSYAISGFLVTAITITLAVVQPVAGWLQDRGGWTVGASWCILLTAIAISCFAF
                     VQNYWLLLCLAVVAGLGNAMFHPNAYQQIYQFSTPANRGTLLSVFSVGGSFGYGAAPL
                     VTGALLVWAGLPALIYLIIPGIVVAFIISRFPQKPVHEPSSSVEYSREENRANWGSAG
                     LMLGISSLRTWVYYGFLTFATVYLTTYAGIEYFFATVLVTGMFYAGMIATLVAGVSSD
                     KLGRKEILLISYACSVPAYLGIFLLPSPFSLLSLLVAGFFLMAPATIEIATVQEMMPG
                     SVGLASGIVIGIPQALSAVAIVVIGVVADTIGMPEALAIQSVLMVAAVVLCVFLPYPL
                     RLRRNDA"
     gene            complement(1453..2055)
                     /locus_tag="O0X37_04695"
     CDS             complement(1453..2055)
                     /locus_tag="O0X37_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="METTL5 family protein"
                     /protein_id="HJK62251.1"
                     /translation="MKLRQLEMQLQKVRGFSHPSAEAEQYMTPAPLAARMLFHAAMNG
                     DIAGVRVCDLGCGTGMLSIAAALLGADVIAVDFDPGALAVARENAELFDVDITFIEAC
                     INDADLGIAANTVVMNPPFGAQKEHADRPFIDTALQIAPVSYAILNAGSIPFVASYVR
                     GRAEIAEKISAQLKIPRQFAFHTRESLEIPVEIVHLERTL"
     gene            complement(2059..3006)
                     /gene="dph2"
                     /locus_tag="O0X37_04700"
     CDS             complement(2059..3006)
                     /gene="dph2"
                     /locus_tag="O0X37_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043364 - glycyl-radical enzyme activating
                     activity [Evidence IEA];
                     GO_process: GO:0017183 - peptidyl-diphthamide biosynthetic
                     process from peptidyl-histidine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphthamide biosynthesis enzyme Dph2"
                     /protein_id="HJK62252.1"
                     /translation="MIPLSDLIDDLHARGAKKVAVQLPEGLKRGTAMLARALRDEGFE
                     VMVCGDACWGACDLALDALADADVLVHVGHTPVTPEKNVIYIPFRQDIDLEILHSAVS
                     PLKNFSTVGLVTTIQHAHQTREMADWLQDHGIHVVVGEGSDRTPEPGQVLGCTYAAAK
                     SAGADAYLFVGTGVFHAIGVSLATGKPTFALDPYGDGGVQEVSADRLLRKRFVQIEKA
                     KSAKTFGILLSSKSGQCRKELAKRLETLSPDASVVLIREVSEMQLRNLGFDAYVNTAC
                     PRLALDDQSRFPMPVLSPAEFEIVLGLRSWDDYTIDEIE"
BASE COUNT          870 a          853 c          713 g          621 t
ORIGIN      
        1 cactgagggc aacgctgaac aaagccacgg taacggaaac agcgacacca acagagatga
       61 caaccgtccc cacagaaaca gtgacaacaa acccgacgac atctacaccg accgcaccgc
      121 cgactaaagc ccccatacca gtactgggag cgttactcgg gtgtgccgca gcagttcttc
      181 tgataaggaa aaactgaact cttttatttt tgtgatcacg gacaggatag caaaactgct
      241 ccgtgaatca gatccgctat gcatcatttc tccgcagtct cagcggatac ggcaagaaga
      301 cgcacagtac caccgccgca accatcagta cagactggat tgctaatgct tcaggcatac
      361 cgatagtgtc agcaacaaca ccgatcacca caattgcaac agcactcaac gcctgcggaa
      421 ttccgatcac aatgccggat gcaagaccta cacttccggg catcatctcc tgtacggttg
      481 caatctcgat cgttgccgga gccatcagga aaaatccggc gacaagaagg gacaacagag
      541 agaacgggga gggtaacaga aaaattccca gatacgcagg aacagagcat gcatacgata
      601 tcagaagaat ttcctttctg ccgagtttat cagaactaac acctgcgaca agggttgcaa
      661 tcattcccgc ataaaacatg ccggtaacca agacggttgc aaagaagtac tcaatgcccg
      721 catatgtggt cagatacacg gttgcaaacg taagaaaccc ataatacacc caggtgcgca
      781 gtgaactaat tccaagcatt agtcctgcac taccccagtt cgcacggttt tcttcccggg
      841 agtactccac ggacgaagaa ggttcatgta ccggtttttg cgggaaacgg gagatgatga
      901 acgcgacaac gataccagga attatcagat atatgagagc cggcaaacct gcccacacca
      961 ggagagcacc tgtaaccagc ggtgctgcac cataaccgaa actaccgccc accgagaaca
     1021 ccgataagag cgtcccgcgg ttggcaggag tggaaaactg atagatctgc tgatatgcat
     1081 ttggatgaaa cattgcgttg ccgagaccgg caacaactgc aagacacagc agcagccagt
     1141 agttctgtac aaacgcaaag cagctgatgg caattgccgt cagcaggata caccagcttg
     1201 caccaactgt ccagcccccc ctgtcctgaa gccatcctgc gaccggctgg acaacagcga
     1261 gagtgatcgt aatggctgtt accagaaagc ccgagattgc atacgaggtg attccctgtt
     1321 cagcaaagat tggcagtaat accggtagga ctgccgagat aaccgggatg taaaaatcaa
     1381 cggcaccgtg tcctgcggca agaaccgcaa tccgttttgc aaggctgcga tgcgacgcac
     1441 acggaagatc agtcataaag ttctctccag atgcacaatt tcgacaggaa tctcaagaga
     1501 ttctctggtg tgaaacgcaa actgtctggg aattttcagt tgtgcagaaa ttttttccgc
     1561 aatttccgca cgccctctga cgtacgatgc aacgaaagga atactgcccg cattcagaat
     1621 tgcataactg acaggcgcaa tctgaagagc cgtatcaata aacggacgat cggcatgttc
     1681 cttctgtgct ccaaacggcg gattcatcac caccgtatta gcggcaatgc caagatctgc
     1741 atcgttaatg caggcttcaa taaaggtgat atcaacatcg aacaactccg cattttcccg
     1801 tgctactgca agagcaccag gatcaaaatc aaccgcaatg acgtcagcac caagcagagc
     1861 ggcagcaatg gaaagcatac ctgtaccgca tccaagatca cagacacgga cacctgcaat
     1921 atccccgttc attgctgcgt gaaacagcat gcgggctgca agcggtgcag gcgtcatgta
     1981 ctgttctgct tcggcagatg gatgagaaaa tcctcttacc ttttgcagct gcatctcaag
     2041 ctggcgaagt ttcatggttt actctatttc atcgattgtg tagtcatccc atgaccggag
     2101 cccgagtact atttcaaact ccgcaggaga cagaaccggc atcgggaacc ggctctgatc
     2161 atccagggca aggcgcggac atgcggtgtt cacatatgca tcaaaaccaa ggtttcgcag
     2221 ctgcatctca gacacctcac ggatgaggac gactgacgca tcaggagaaa gggtctccag
     2281 ccgctttgca agttcttttc tgcactgtcc cgatttgctg gaaagcagga tgccaaatgt
     2341 ttttgccgat ttcgcttttt cgatctgcac gaaccgtttc cggagcagac gatccgccga
     2401 aacctcctga acacctccgt caccataagg atccaacgca aaagttggtt ttcctgttgc
     2461 aagcgagaca ccgattgcat gaaagacacc ggttccaaca aacaggtacg catctgcacc
     2521 cgcggatttt gcagctgcat acgtacatcc aagcacctgt cccggctccg gagtgcgatc
     2581 agatccctcg ccaaccacca catggattcc gtgatcctgc agccagtcag ccatctcccg
     2641 tgtttggtgt gcgtgctgga ttgtggtcac cagaccaacc gttgagaaat tcttgagagg
     2701 agacacagca gaatggagaa tctccagatc aatatcctga cggaacggaa tgtagatcac
     2761 attcttttcc ggtgttaccg gcgtgtgacc gacatggacc aacacatcag catccgcaag
     2821 ggcatcaagt gccagatcac acgcccccca gcatgcatca ccgcagacca tcacctcaaa
     2881 cccttcgtca cggagtgctc tggcaagcat tgcagtccct cgtttcaacc cttccggtag
     2941 ctgaactgca actttttttg caccacgtgc atggagatcg tcaatcagat cagaaagcgg
     3001 aatcacagat cacacctccc aaaaacaccg gacagtcgca tagtaatact atactac
//
LOCUS       DALUAJ010000222         5950 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_43113,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000222 DALUAJ010000000
VERSION     DALUAJ010000222.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43113"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(153..1148)
                     /gene="purM"
                     /locus_tag="O0X37_04705"
     CDS             complement(153..1148)
                     /gene="purM"
                     /locus_tag="O0X37_04705"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HJK62253.1"
                     /translation="MSTKYSYKDAGVDINLEADGVKALIHQLSYKRSGEHGMLGGVGH
                     FAGLIDFGSKVLSLCTDGVGTKMRVADDLGDWTTVGIDCIAMNVNDMYVMNIEPVAFV
                     DYIATEGINTEQMIQIGVGLNEGAKRANMNIVGGETATLKGMINGLDLAGTCLGVQDR
                     DQVVTGEKVVPGDVIIGVASTGVHSNGYTLARKIATENGGYGVKLPSGKTVGEALITP
                     TRIYSEVLDVCRAAEVHGMCHVTGGGLLNFLRISDYGFSIEDPMPVPEILQWIKEKGE
                     LETNELYRTFNMGMGFAFIVPEASVAKILAMVDGAKVVGHVIPEHKVLLKGEEIN"
     gene            complement(1145..2542)
                     /locus_tag="O0X37_04710"
     CDS             complement(1145..2542)
                     /locus_tag="O0X37_04710"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HJK62254.1"
                     /translation="MRLVMKFGGTSVGDEKCIANVVNIIKESREEGNEIAVVVSAMTR
                     VTDQLIATAEEVINCKQKPALDAFITSIRSRHMKVLEAVAPDYVDEIGEYLDSRLNKL
                     SNILLAVHNLRELTPRSKDYIISFGERLSAPIISAALRQAGIPSCYMSGCDAGILTDG
                     VHNGATALPESYARIQARVGALLTEEVPVVMGYMGCAADGAVTTLGRSGSDYSGAIVG
                     AGIDADEILIWTDVDGVMTTDPRIIPEARVINSISFLEMMEMSYFGAKVIHPRALVPA
                     MQKNIPVRVKNTFNPSHPGTVVIREPHADKRIVKAISLIQNSCLIKVGGAVMAGRPGV
                     AGEIFTTLAEAGVNVMLISQGSSEMTISMIISEEQLDAALSALAEVKQRGCIREFGFD
                     RDVAVVSVVGAGMAGTYGTLGRIFHGLGVAGINVMMLSQGSEVNVSFVVKRADGVKAV
                     RAIHEEYHLEEEEEA"
     gene            complement(2636..2821)
                     /locus_tag="O0X37_04715"
     CDS             complement(2636..2821)
                     /locus_tag="O0X37_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62255.1"
                     /translation="MKNVEMNLEGNILTIKVDITKEFGVSGSGKSIIIASTEGNQSIA
                     PDHDVKIGLNVYRKNTN"
     gene            complement(2876..3337)
                     /gene="ribC"
                     /locus_tag="O0X37_04720"
     CDS             complement(2876..3337)
                     /gene="ribC"
                     /locus_tag="O0X37_04720"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011991066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HJK62256.1"
                     /translation="MIIGVVDTTFARANMGAFAVDELKKHTSARIIRVTVPGVKDLPV
                     AAKKLIEEERCDIVMALGMPGGKDQDKICAHEASQGIMMAQLMTNKHIIEVFVHEDEA
                     KDDAELAWLLEQRTREHAVNAVLMLTRPQDMTKLAGTGQRQGFADVGPARR"
     gene            complement(3334..4467)
                     /locus_tag="O0X37_04725"
     CDS             complement(3334..4467)
                     /locus_tag="O0X37_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HJK62257.1"
                     /translation="MYNETLLMMPGPVPMPESVRNAMTKQAINHRSKEFGDCYADIVR
                     TLKPVFGTKNDILVLSGSGTAGQEAAIGSFAKGKKVASLVNGKFGERLGQIAAIYGES
                     VMIESEWGHPLNLEGLKEALENGAEVVTLVHNETSAAILNPAEEVGKLARKYDALFIL
                     DAITSIGGDVVEADKWGADVAIVGSQKCLAAPAGLAAVSVSQRAWDRLSETRPFYLDL
                     KKAKKSADGNPMETPTTPAVPLFLALREACRLIEEEGIEHRIARHHKMSAAVRAAGAA
                     WGLDLVPQVDALHKASNTVTGFFYPEGIEDSQIRGVCKKMGIEFAGGQDRFKGKIFRI
                     GNMGIIDTPEILATVAAVQMAFKKTGYKLEGDGLAAAVEVLSA"
     gene            4517..4909
                     /locus_tag="O0X37_04730"
     CDS             4517..4909
                     /locus_tag="O0X37_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HJK62258.1"
                     /translation="MADVAVIAGSASDQAIIDKVVNTLAQYGISYDMQILSAHRDAEK
                     LDNYVKASDAKVFICIAGMSAALPGVVAARTPKPVIGVPVSGKIAGGLDALLSIAQMP
                     KGVPVACMAVDGGENAAHMAHRILSLLP"
     gene            complement(5044..5940)
                     /locus_tag="O0X37_04735"
     CDS             complement(5044..5940)
                     /locus_tag="O0X37_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011447106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HJK62259.1"
                     /translation="MDKKVLHGVAGLCLVFAVLMAGCVDTPADDGKKTYVVGIDGEYE
                     PFSYLGDNGEFIGFDVDSVKWIAEQKGFNVEIQAVAWDGIIPALQTEKIDMVYSGMSI
                     TPDREEKVNFTIPYWQVNQSVVALADSEVTMDQFTNGTIVIGVQRSCSADQWMQKAED
                     EGGLFGEEKYNQLVKDGNIKLYDSFPMSMVALEQGLVDVVIFDDANVESYIQGKSQFA
                     MVGVIETGEYYAVAVRKDDNDLRELMNEGLTELMASDKWDELIAQYIVEDVAEPTVEP
                     TTNETVVENVTAVETTPAETTA"
BASE COUNT         1337 a         1608 c         1451 g         1554 t
ORIGIN      
        1 gggaccgcag gagtctctcc attatcctcg gaattcacac agcctgcggc aagtaccaaa
       61 cagccgagta gcacagctac tgcaaacaga tgcacatact tcatacggaa atatcaacgg
      121 gagaacagat agaaattgcg gtaaaaaaag agttagttga tctcttcgcc ctttaacagg
      181 actttgtgct cgggaataac atgcccgaca acttttgcac cgtcaaccat cgccagaatc
      241 tttgcgacac ttgcttcagg cacaataaat gcaaagccca tacccatgtt aaatgtccgg
      301 tacagctcgt tcgtctcaag ttcgcccttc tctttgatcc actgtaaaat ctccggaacc
      361 ggcattggat cctcaatgga aaatccgtag tcactgatcc gcaggaaatt taagaggcca
      421 ccgccggtta catggcacat gccgtgaacc tcggctgcac ggcagacgtc cagtacctcg
      481 gagtagatac gggtcggcgt aatgagtgcc tcgccgaccg tctttccgga aggaagtttg
      541 accccgtagc cgccgttttc ggttgcgatc tttcttgcca gggtataacc gtttgagtga
      601 acaccggtac ttgcaacacc aatgattacg tctcccggaa caaccttctc accggtaacc
      661 acttggtcgc gatcctgcac gccaagacag gttcctgcca gatccagacc attgatcatt
      721 cctttcagcg ttgccgtttc cccgccgaca atgttcatgt tcgcacgttt tgcaccttcg
      781 ttcaatccga caccgatctg gatcatctgt tcggtgttga tgccttcggt tgcgatgtag
      841 tcgacgaatg ccaccggttc gatgttcatc acgtacatgt cgttgacgtt catcgcaata
      901 caatcaatcc cgaccgtcgt ccagtcaccg agatcatcgg caacccgcat ttttgttccg
      961 acaccatcgg tacagaggga gaggactttg gatccgaaat caatcagacc ggcaaagtgt
     1021 cccactccgc caagcatacc gtgctcgccg gagcgtttgt aactcagctg gtggatcagt
     1081 gccttgacgc cgtcggcttc aaggttgatg tcaacacctg cgtccttata ggaatatttt
     1141 gtactcatgc ttcttcctct tcttccaggt ggtactcttc gtggatcgcc cggaccgctt
     1201 tcactccgtc agcacgtttg accacgaagg aaacgttcac ttctgatcct tgtgaaagca
     1261 tcatcacgtt gataccggcg acgccaaggc cgtggaagat acgtcccagc gttccatagg
     1321 ttcctgccat tccggcaccg acaaccgaaa cgacggcaac atcacgatca aatccaaact
     1381 cacggatgca tcctctctgc ttcacctctg caagtgccga gagtgcggcg tccagctgct
     1441 cttcgctgat gatcattgag atggtcatct cggatgatcc ctgcgagatc agcatcacat
     1501 tgacaccggc ttcagcaagc gtcgtgaaga tctctccggc aacacctggt cgtcctgcca
     1561 tgactgctcc cccgactttg atcagacagc tgttctgtat caaggagatt gcctttacaa
     1621 tcctcttgtc tgcgtgcggc tcacgaatga caaccgtacc agggtgggat ggattgaagg
     1681 tgttcttgac acgaacaggg atgtttttct gcattgccgg tacgagtgcc cgcgggtgaa
     1741 tgacctttgc gccgaagtag gacatctcca tcatctcaag gaaagagatc gagttgataa
     1801 ccctggcttc cgggatgatg cgggggtccg tggtcataac accgtcaaca tcggtccaga
     1861 tgagaatctc atcggcatcg atgcctgcac ccacaattgc tcctgagtag tcggaaccgc
     1921 tgcgtcccag agtggtgact gcaccatccg ccgcacatcc catgtatccc atcacaacag
     1981 gaacttcctc ggtcagaaga gcaccgacgc gtgcctgaat ccgagcatag ctttccggaa
     2041 gtgcagttgc gccgttgtgc acaccgtctg tcagaattcc tgcatcacag ccgctcatgt
     2101 aacagcttgg aatacctgcc tgccggagtg ctgcgctgat gatcggagcg gacaaccgct
     2161 caccaaacga aatgatgtag tccttggacc gcggggtaag ttcgcggagg ttgtggactg
     2221 caagcaggat gttggaaagt ttgttaagcc gggaatcgag atactcccca atctcgtcca
     2281 catagtctgg agcaaccgct tccagaacct tcatgtgccg gctgcggatc gatgtgatga
     2341 acgcatcaag ggctggcttt tgtttgcagt tgatgacctc ttcggcggtt gcgatcagct
     2401 gatcggttac gcgggtcatg gcagagacca ctactgcgat ttcgtttcct tcctcgcggg
     2461 attccttgat gatgttgacc acattggcaa tgcatttttc atcgccgacg gatgttcctc
     2521 caaacttcat tacgagtcgc atcggattta gcacctatga taatactgtt ctatcaggtg
     2581 ggtgttgtga ggtgatataa attgtccacg ggagtgtggt gtctgaaaaa aagagttagt
     2641 tggtgttctt gcggtacacg tttaagccga ttttgacatc gtggtcaggg gcaatcgact
     2701 ggtttccctc ggtggatgca attatgatgg actttccgga accggagaca ccaaactctt
     2761 tggtgatatc tactttaatg gtcaggatat ttccttcaag gttcatctct acatttttca
     2821 taagagtacc tctgcaggaa attatatagc ctttcctgaa atttttcaaa aaaaattatc
     2881 tccgggcagg acccacgtca gcgaatccct gacgctggcc ggttccggcg agttttgtca
     2941 tatcctgcgg gcgtgtcagc atcaggacag cgttgaccgc gtgctcgcgg gtccgctgtt
     3001 ccagcagcca cgcaagttct gcatcatctt ttgcctcgtc ctcgtgaacg aacacctcaa
     3061 tgatgtgctt attggtcatc agctgtgcca tcatgatacc ctgcgaggct tcatgggcac
     3121 agattttgtc ctggtccttt ccgcccggca tgccaagcgc catgacaatg tcgcagcgtt
     3181 cctcttcaat gagttttttt gcagcgaccg ggagatcttt cactcccggc actgtcacac
     3241 ggatgatccg ggcacttgtg tgctttttca gctcatcaac ggcaaacgcg cccatgttgg
     3301 cgcgggcgaa tgttgtgtcg actacgccga tgatcatgcg gacagaacct cgactgcagc
     3361 ggccagaccg tcgccttcga gtttgtatcc ggtcttcttg aatgccatct gtactgctgc
     3421 aacggttgca agaatttccg gtgtatcgat gatgcccatg ttgccaatcc ggaagatttt
     3481 gcccttgaac ctatcctgtc ctccggcaaa ctcaatgccc atcttcttac agacgccgcg
     3541 aatctgggag tcttctattc cttccggata gaaaaatccg gtgacggtgt tcgatgcctt
     3601 gtgcagtgca tcaacctgcg gaaccagatc aagtccccat gcagcaccgg cagcacggac
     3661 cgcggcagac attttgtggt ggcgtgcaat gcggtgttcg attccctctt cctcaataag
     3721 tctgcatgct tcacgaagcg cgaggaagag cgggaccgcc ggggttgtcg gtgtttccat
     3781 cgggttaccg tcggctgatt tctttgcctt ctttaagtcg agatagaagg gacgtgtttc
     3841 agaaaggcga tcccatgcac gctgggatac ggaaactgcg gccagtcctg caggagccgc
     3901 aaggcatttc tgggatccga cgattgcaac atccgctccc catttatccg cctcgacaac
     3961 atctccgccg atcgaggtga ttgcatcaag gatgaagagt gcatcgtatt tacgtgcgag
     4021 tttacccacc tcttcagcgg ggttgaggat tgcggcagag gtctcattgt ggacgagggt
     4081 tacgacctct gcaccgtttt ccagggcttc cttgagtcct tccaggttta acgggtgtcc
     4141 ccactcggac tcgatcatca cggactctcc gtagattgcg gcaatctgtc cgagccgctc
     4201 tccaaacttt ccgttaacga gggatgcgac tttcttgcct ttggcaaagg atccgattgc
     4261 ggcttcctgt cctgcggttc ctgatccaga gagaacaagt atatcgtttt tcgtaccgaa
     4321 caccggtttt aaggtcctga caatgtctgc gtagcagtca ccgaactcct tgctgcggtg
     4381 attgattgcc tgctttgtca ttgcgtttct gacggactcc ggcatcggaa ccggtcccgg
     4441 catcatgaga agtgtttcgt tatacatggt atcagggatg tttgtacata tatgtgaaga
     4501 gacaatactt atattcatgg cagatgttgc agttattgcc ggatccgcat cagatcaggc
     4561 tattatcgat aaagttgtga atacgctcgc gcagtatggc atctcttatg acatgcagat
     4621 tctctctgcc caccgtgatg cggaaaagct ggacaattat gtaaaggcaa gcgatgcaaa
     4681 agtgttcatc tgtattgcag gaatgtctgc ggcacttccg ggtgttgttg cggcaagaac
     4741 accaaagccg gtgatcggtg tgccggtgtc gggaaagatt gccggaggtc ttgatgcctt
     4801 gctttctatt gcccagatgc caaaaggggt tcctgttgcc tgtatggccg tcgacggcgg
     4861 ggagaacgct gcacacatgg cccatcggat cttatcgctt cttccctgat aattttacat
     4921 cacttttttc ctgaatggtc agctatcatt ggtctaactg aacacatttt ctcttggaat
     4981 agctgtcctg atcttgtgga cagataaaaa aagaaaatct gctgaaggca gattatctta
     5041 cacttatgcg gttgtctctg ctggagttgt ctcaacagca gttacgtttt ccacaacagt
     5101 ctcgttcgtc gtcggttcaa cggtcggctc agctacatcc tctacgatgt actgtgcaat
     5161 cagttcatcc cacttgtcgg atgccataag ttcagtgaga ccttcgttca tcagctcacg
     5221 gagatcgttg tcatccttac ggactgcaac tgcgtagtac tcaccggtct caataacacc
     5281 aaccatggcg aactggctct tgccctggat gtagctctcg acatttgcat cgtcgaagat
     5341 aaccacatca acaagtccct gctcaagtgc aaccattgac atcgggaagg agtcatacag
     5401 cttaatgttg ccgtccttta ccagctggtt gtacttctcc tctccgaaaa gtccgccttc
     5461 atcttctgct ttctgcatcc actggtcggc ggaacagctg cgctgtacac caatcacaat
     5521 ggtaccgttg gtaaactgat ccattgttac ctcagagtct gccagtgcta caacactctg
     5581 gttgacctgc cagtacggga tggtgaagtt tactttctct tcacggtcgg gagtgatgga
     5641 cataccggag tataccatgt caattttttc agtctgcagt gcaggaatga taccatccca
     5701 tgcaacagcc tggatctcga cattgaatcc tttctgctct gcaatccact ttacagagtc
     5761 aacatcaaaa ccgatgaact cgccgttgtc gccaaggtat gagaacggct cgtattctcc
     5821 atcaatacca acaacatagg tttttttgcc gtcatcagca ggagtgtcta cacaccctgc
     5881 catcaggaca gcaaaaacaa ggcacagccc tgctacccca tgcaacactt ttttatccat
     5941 cctatagtga
//
LOCUS       DALUAJ010000223         2833 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_43264,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000223 DALUAJ010000000
VERSION     DALUAJ010000223.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43264"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="O0X37_04740"
     CDS             <1..415
                     /locus_tag="O0X37_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HJK62260.1"
                     /translation="SLADALTKYNNIRLHTIAPEGLDLPNHLKQDLTDAGMEIIVHEN
                     IEEALPELDVLYVTRLQRERFPDPAAFANFAATYRITPDLLERAKPSMIVLHPLPRVD
                     EIDPAVDSLPCAKYFQQAHYGVPVRMAMLHEVMSS"
     gene            412..879
                     /gene="pyrI"
                     /locus_tag="O0X37_04745"
     CDS             412..879
                     /gene="pyrI"
                     /locus_tag="O0X37_04745"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HJK62261.1"
                     /translation="MKRSVSEGIVISPIKNGTVIDHITPGEGLTVIRILGIQDGTNVT
                     FTVATNVTSSRGGRKDMVKIENRELVKSEVDKIALIAPDAKISIIRDFKVVAKKPVEV
                     PSDIVGVIKCPNPNCISNTREPAQSKFFVRPRGVRCQYCDTFISREMDIGDYI"
     gene            complement(901..1770)
                     /locus_tag="O0X37_04750"
     CDS             complement(901..1770)
                     /locus_tag="O0X37_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HJK62262.1"
                     /translation="MSPRLVSEFRSRFAASGMTEDLIRDFQNEVMEFYETKGRHDMEW
                     RLRFDPYRIVVSEVMLQQTQVPRVAVMYPAFIEKFPDFASLAAAPQAELLAAWQGMGY
                     NRRALNLQKLAVRVQEEFGGILPEDPKTLATLPGIGPATSCSIAAFAFNRTVVFIETN
                     IRRVFIHYFFSDTAVVDDRDLFPLVEACLLPGRTREWYWALMDLGTALKTSVPNPNRR
                     SRQYVKQSAFDGSDRQIRGALLRLLLNGDAEDAETLAKAAGASHDRVSRILGEMCREG
                     FFVQEEGGAYRIR"
     gene            complement(1814..>2833)
                     /locus_tag="O0X37_04755"
     CDS             complement(1814..>2833)
                     /locus_tag="O0X37_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62263.1"
                     /translation="VIAIFLYYILSTLYPIDKIIGRMYPFIGGLLILITAAVCIACIP
                     YADLIPDITMTPEGIAAAFTAHPEGQPLIPMLFITIACGILSGFHATQSPIVSRTMQS
                     EKDGRKIFYGMMIAEGIIAMIWAAVTSILFALEPEMLASSNGNQIISTAVGVVLPAAL
                     ASLSLIVFIFLAVTSGDTALRVLRTSVADFLKIDQTPAKLRVLVLLPLVLLISGLLIW
                     SNLSPTGYAVLWNYFAWFNQLIASCALLLATAYLASRAKPYLITAVPGTLILFMCISY
                     ILWVSPAHLAGAPLGLGLPLEVSYVVAAVLSVILCAAAVLTGRRLGNDPAFSADLPAT
                     YVDES"
BASE COUNT          688 a          712 c          813 g          620 t
ORIGIN      
        1 ctcccttgct gatgcgctga caaagtacaa taacatccgt ctgcatacga ttgctccgga
       61 aggccttgat ctgccgaatc atctgaagca ggatctcacc gatgccggca tggagattat
      121 tgtgcatgaa aacattgagg aggctcttcc cgagcttgat gtgctgtatg ttacccggct
      181 gcagcgtgag cggttcccgg atccggccgc gtttgcaaac tttgcggcaa cgtaccggat
      241 aacgccggat cttttggaac gggcaaagcc gtcaatgatt gttcttcatc cgcttccccg
      301 ggtggatgag atcgatccgg cggttgacag cctgccgtgt gcaaagtact tccagcaggc
      361 gcattacggt gtgccggtga gaatggcgat gttgcatgag gtgatgagtt catgaagcga
      421 tcggtttcgg aaggaattgt gatctcccct atcaaaaacg gtacggtgat cgatcacatt
      481 acccctggtg aggggctgac ggttatccgt attcttggta ttcaggacgg aacgaatgtg
      541 acgtttacgg ttgcgacgaa cgtgaccagc agccgcggcg gcaggaagga tatggtaaag
      601 attgagaacc gcgaactggt gaagtccgag gtggacaaga ttgctctgat tgctccggat
      661 gcaaagatca gtattatccg ggatttcaag gtggttgcca agaagcctgt ggaggttccg
      721 tctgatattg tgggtgtgat taagtgcccg aacccgaact gtatcagcaa tacccgcgaa
      781 ccggcgcaga gcaagttttt tgtgcgcccg cggggtgtcc gctgccagta ctgcgatacg
      841 ttcatctccc gcgagatgga cataggagac tacatctaat ctttttttgc tgtgccgggg
      901 ttatcgtatc cggtaggcac cgccctcttc ctgtacaaaa aatccttcgc ggcacatctc
      961 tcccagtatc ctggaaaccc tgtcgtggga tgccccagcc gcttttgcaa gggtttctgc
     1021 atcctcagca tccccgttta acagcagacg gagcaatgct ccgcgaatct gccggtctga
     1081 tccgtcaaat gcggactgct ttacatactg cctgctccgg cggttcgggt tggggacaga
     1141 ggttttgagt gcggttccga gatccatcag tgcccagtac cattcgcggg ttcttcccgg
     1201 caaaaggcag gcctcaacca gcgggaaaag atcacggtcg tccactactg cggtgtctga
     1261 gaagaaatag tgaatgaaga cgcgtcgaat gttggtctcg atgaatacca ccgtccggtt
     1321 gaaggcgaag gctgcgattg aacaggaggt ggcgggaccg attcccggca gtgttgcaag
     1381 ggtttttgga tcttccggaa gaattcctcc gaactcctct tgaacacgga cggcaagttt
     1441 ctgaagattc agtgcacgcc ggttgtatcc cattccctgc caggctgcaa ggagttccgc
     1501 ctgtggtgct gcggcaagtg aggcaaaatc cggaaatttt tcgatgaagg ccggatacat
     1561 caccgctacc cgtggtacct gcgtctgctg gagcatcact tccgagacaa cgatgcgata
     1621 gggatcgaac cgcagcctcc actccatatc gtgccgtcct ttggtctcat agaactccat
     1681 cacttcgttc tgaaaatcgc ggatgagatc ctcagtcata ccggacgcgg caaaccggct
     1741 gcggaactcg gagacgaggc gtggggacat tgctgttcag tatctcgctg agaaaaaaga
     1801 aaagggtttg ggatcaggac tcatccacat atgttgcagg cagatccgcc gagaatgccg
     1861 gatcgtttcc gagacggcga ccggtgagaa ccgcagcagc acaaagaatc acgctcagta
     1921 ccgccgcgac cacatacgat acctctaacg gcagacccag tccgaggggt gctcctgcca
     1981 gatgtgccgg actcacccac agaatgtaac tgatgcacat aaagagaatc agtgttccgg
     2041 gaactgccgt gataagatac ggctttgccc tgcttgccag atatgccgtg gcaagcagca
     2101 gagcacagga agcgatcagc tgattgaacc aggcaaagta gttccacaac accgcatacc
     2161 cggtcggcga caggtttgac cagatcagaa gaccgctgat aaggagaaca agcggaagca
     2221 gtacgagaac acggagtttt gccggagtct gatcgatctt caagaagtct gcaaccgagg
     2281 tccggagaac ccgcagtgcc gtatcgccgc tggtgaccgc aaggaagatg aatacaatga
     2341 gggaaagtga ggccaaagct gccggaagta caacccctac tgcagtggag ataatctggt
     2401 ttccgtttga ggaggcaagc atctctggct caagggcaaa cagtatcgag gttaccgcag
     2461 cccagatcat cgcgataatg ccttctgcaa tcatcatgcc gtagaagatc ttccgcccgt
     2521 ccttttcgga ctgcattgtt cttgagacga ttggagactg ggttgcatgg aacccggaga
     2581 gaataccgca ggcaatcgta ataaacagca tcgggataag cggctgacct tcgggatggg
     2641 cagtgaacgc agcagcgatc ccttcgggtg tcatggtgat atcagggatg agatctgcgt
     2701 aaggaataca ggcgatgcag accgcagcgg ttatcaggat cagaagaccg ccaatgaacg
     2761 gatacattct gccgatgatt ttatcgatcg ggtacagggt ggaaagaatg tagtagagga
     2821 atatggcaat tac
//
LOCUS       DALUAJ010000224         2034 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_43423,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000224 DALUAJ010000000
VERSION     DALUAJ010000224.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43423"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="O0X37_04760"
     CDS             <1..598
                     /locus_tag="O0X37_04760"
                     /inference="COORDINATES: protein motif:HMM:NF018546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase A24"
                     /protein_id="HJK62264.1"
                     /translation="PTTTAISVLVLSVVICILMWGCAKAGLFGLADAKAMILLAATVP
                     VNPFAVWLFPSLALSTLVNAGVIALLVPAALLIRNTIRKEHAPFWLMCSGMQVAGDSL
                     TRYFGFVAEEITDGDPISRRFLPAASSIRALKENSACSIRNLREHPERFEKELELYRK
                     AGRVWITYGIPFMIPITIGYILALAGFSLPDAVLSLFI"
     gene            complement(591..1487)
                     /locus_tag="O0X37_04765"
     CDS             complement(591..1487)
                     /locus_tag="O0X37_04765"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HJK62265.1"
                     /translation="MGSEMIISASRRTDIPAWFSEWFCNRLCEGFCYVRNPYSQKITR
                     VSLMREDTDVIVFWTKNAEPMMQRLSAVDASGIPYYFQYTVTPYGSDVEPGLTKRDCI
                     KNFLELSEMLGRDRVLWRYDPVLITEKYSVEFHADAFAKLCELFSEATCRCTISFVDA
                     YRASPFRTCTFDEMIRLAEIFSATAKRFGLPLVSCAEEIDLDRFGISHASCVDKEICE
                     SLCGRPLPVKRDRNQRTACGCVESTDIGAYGTCRCGCRYCYACGRRTAAAHDPASPLI
                     SGWPKDNEPVFPRSSGQTTLFR"
     gene            complement(1477..>2034)
                     /locus_tag="O0X37_04770"
     CDS             complement(1477..>2034)
                     /locus_tag="O0X37_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HJK62266.1"
                     /translation="PDLFPKEICFMISEVDLLADPGRLEMIAGWCMEFSTIKRIIFHI
                     ASKDPAALEGKMPSLQSFGGSASVRLSMPSGETTFGSGTPEILIVLGRSGREEITEAI
                     AAIAREGVNPETITEETIESHLLYQVNPDFVIKTGGSHLTDFLIWQSVYSELFFTDIN
                     WCRFRRLDLLRALRDYQSRVRRYGQ"
BASE COUNT          514 a          580 c          482 g          458 t
ORIGIN      
        1 cccgacaacc accgcaataa gcgtacttgt actctcagtt gttatctgca tactgatgtg
       61 gggatgtgca aaagctggtc tgttcggtct ggcggatgca aaagcaatga tcctcctcgc
      121 cgcaaccgta cccgtcaatc cgtttgccgt atggttgttt ccctcgcttg ctctctcaac
      181 cctcgttaat gcaggcgtta tcgcacttct cgttcccgca gcactgctga tccgaaacac
      241 catccgcaaa gaacacgccc cgttctggct catgtgttcc ggcatgcagg tagcaggtga
      301 ctcgcttacc cggtacttcg gttttgttgc agaagagatc accgacgggg atcctatctc
      361 ccgcagattt cttcctgctg caagttcgat tcgtgccctg aaggaaaact cagcctgttc
      421 aattcgaaac ctccgtgaac atccagaaag atttgaaaaa gaattggagc tctaccggaa
      481 ggcagggaga gtctggatca cctacggcat tccgttcatg attccgataa ccatcggcta
      541 tattctggca ctcgccggat tcagtctgcc ggacgccgtt ctctccctct tcatctaaag
      601 agagttgtct gaccagaact acgcggaaaa accggttcat tgtccttcgg ccagccggaa
      661 atcagcgggg atgcggggtc atgtgccgca gcagtcctgc gtccgcaggc ataacagtac
      721 cggcatccgc acctgcaggt accgtatgcc ccgatatcag tactctcaac acagccgcag
      781 gcagtacgct ggtttcgatc acgctttact ggaagcggac ggccgcagag agactcacag
      841 atctctttgt ccacgcagga tgcatggctg ataccaaatc tgtccagatc gatctcctca
      901 gcacaggaga caagcggcag accaaaccgt tttgccgttg cagaaaagat ctcggcaagc
      961 cggatcatct cgtcaaaagt acaggtacga aacggagagg cgcgatacgc atccacaaaa
     1021 ctgattgtac agcgacaggt tgcctccgaa aatagttcac acagctttgc aaacgcgtct
     1081 gcatgaaact ccaccgagta tttttctgtg ataagaaccg gatcgtaacg ccacagcaca
     1141 cgatctcttc caagcatttc cgaaagctcg agaaaatttt tgatacagtc acgcttcgtt
     1201 aaaccaggtt ctacatcaga accgtagggc gttacggtgt actgaaaata gtagggaatt
     1261 ccagatgcat ccaccgcaga aagcctctgc atcatcggct ctgcattttt tgtccagaac
     1321 acaatcacgt ccgtgtcctc acgcatcagg gatacacgtg tgatcttctg tgaatagggg
     1381 ttgcggacgt aacaaaaccc ttcgcaaaga cggttacaga accactccga aaaccatgca
     1441 ggaatgtccg tacgccggga tgcagagatg atcatctcac tgcccatacc ggcgaacccg
     1501 tgactggtaa tcacggagtg cccgcagaag atccagacgg cggaacctgc accagttgat
     1561 atccgtgaaa aacagctcag agtacaccga ctgccagatc agaaaatccg tcaggtggct
     1621 gccgccggtc ttgatgacaa agtcaggatt cacctgatac aaaagatgtg actcgatcgt
     1681 ctcctccgtg attgtctcag gattaactcc ttcccgtgcg atcgcggcaa ttgcctctgt
     1741 gatctcctcg cgcccgctgc gcccgagaac gatcaggatc tcaggggtcc ccgaaccaaa
     1801 cgtagtctct cccgacggca tactcaggcg gactgaggcc gaacctccga aagactgcaa
     1861 agacggcatc tttccctcaa gtgctgccgg atcctttgat gcaatatgaa atataatccg
     1921 tttgattgtg gaaaactcca tgcaccaccc tgcaatcatt tccagtctgc cgggatctgc
     1981 aagaagatcc acttcgctta tcataaagca gatctccttt ggaaacagat cagg
//
LOCUS       DALUAJ010000225         1890 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_43522,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000225 DALUAJ010000000
VERSION     DALUAJ010000225.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43522"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="O0X37_04775"
     CDS             complement(<1..862)
                     /locus_tag="O0X37_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012616781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HJK62267.1"
                     /translation="MPTSKELDEKYYMPAFGRDMEIVLGKDCHVWDSTGKKYLDLVAG
                     IAVCSTGHCHPAVTTAICKQAQTLIHCSNLYYVPGQAELAEKLSKASGLGKVFFGNSG
                     AEANDAAMKLAIRATGRHEFVSFEHDFHGRTVGSLACTHKPAIREPFEPLGIPCKFSP
                     YGDIEALKEFVTKKTAAVILEPIQGETGVIIPPDDFLPGVRDICDDAGALMICDEVQT
                     GMGRTGKWFAFQHSSIQPDIISLAKGVASGIPMGAIIAREGLEFTRGEHGSTFAGGPI
                     ACAAGNATFDV"
     gene            complement(868..1863)
                     /gene="guaA"
                     /locus_tag="O0X37_04780"
     CDS             complement(868..1863)
                     /gene="guaA"
                     /locus_tag="O0X37_04780"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HJK62268.1"
                     /translation="MIRNFLYGVCGCTGTWHMSSFVDNMVAHLREKIGDKKVLCALSG
                     GVDSSVAAVLVHKAVGKQLTCIFVDHGLLRKNEGDEVEEVFRNQYDINLIRVNAEDRF
                     LAKLAGVSDPEQKRKIIGAAFIHVFEEEAKKIGTVDFLVQGTIYPDVIESGPGNAAVI
                     KSHHNVGGLPDHVDFKEIIEPLRILFKDEVRAAGVELGIPEHLVWRQPFPGPGLAIRV
                     IGDITKEKLDILRDADAIFREEVAAAGLGRAINQYFAALTNMRSVGVMGDERTYDYAI
                     ALRAVTTTDFMTADWAQIPYPVLEKISSRIINEVRHVNRVLYDITSKPPATIEYE"
BASE COUNT          443 a          528 c          484 g          435 t
ORIGIN      
        1 gcacatcaaa cgtcgcattt ccggcggcac aggcaatcgg acctcctgca aaggtactgc
       61 cgtgctcgcc tctcgtaaac tccagaccct cgcgtgcgat gattgcaccc atcggaatac
      121 cggatgcaac acccttggca agactgatga tatccggctg gatggacgag tgctggaaag
      181 caaaccattt accggttctg cccataccgg tctgtacctc atcacagatc atcaaagctc
      241 ctgcatcatc acagatgtcg cggacaccag gcaagaaatc atcgggcgga ataatgacac
      301 cggtctcacc ctggatcggt tcaaggataa ccgcggcggt cttctttgtc acaaattctt
      361 tcagtgcctc aatatccccg tagggactga acttgcaggg aataccgagc ggctcaaacg
      421 gctcgcggat tgcaggcttg tgggtacagg caagagaacc cacggttctt ccgtggaagt
      481 catgctcaaa ggagacgaac tcgtggcggc cggttgcacg gatggcaagt ttcattgccg
      541 catcatttgc ctcggcaccg gagtttccga aaaatacctt tccaagaccg gaagccttcg
      601 agagtttttc agcaagctct gcctgacctg gcacatagta gagattagag caatggatca
      661 gcgtctgcgc ctgtttgcag atagcagtgg ttaccgccgg atgacagtgc ccggtactgc
      721 acaccgcaat acctgcgacc agatcaagat attttttccc ggtactgtcc cagacgtggc
      781 agtctttccc caggacgatc tccatgtccc gtccgaaggc gggcatatag tatttttcat
      841 caagttcttt ggaggttggc atacgtatca ctcatattcg atggttgcag ggggtttgct
      901 cgtgatatca tacagaacac gattcacgtg gcgaacctca ttgatgattc tgctggatat
      961 tttttccagt accggatagg gaatctgtgc ccagtccgcg gtcatgaagt cggtcgtcgt
     1021 caccgcacgc agtgcaatgg cgtaatcgta tgtacgttca tcgcccataa caccgacact
     1081 gcgcatgttg gttaaggccg caaagtactg attgatcgca cggccaagac ctgcggctgc
     1141 aacctcctca cggaagatcg catccgcatc acggagaatg tcgagcttct ccttggtgat
     1201 gtcgccgatg acgcggatgg caagacccgg acccgggaac ggctggcgcc acacaagatg
     1261 ttccggaata ccgagctcaa cacccgcagc acgcacctcg tccttgaaga gaatgcgaag
     1321 cggttcgatg atttctttga agtccacatg atccggcaga ccgccgacgt tgtgatggct
     1381 cttgatgacc gcggcatttc cgggaccgga ctcgatgaca tccgggtaaa tggtaccctg
     1441 cacaaggaaa tcaaccgttc cgatcttttt tgcctcctcc tcaaagacat gaatgaacgc
     1501 agcaccgatg atcttccgtt tctgttccgg atcggaaact cctgcaagct ttgcaaggaa
     1561 ccgatcctcg gcatttacgc ggatcagatt gatgtcgtac tggtttctga agacctcttc
     1621 aacctcgtct ccctcgtttt tgcgcagaag accgtgatcc acaaaaatgc aggtcagttg
     1681 tttgccgaca gccttgtgca caaggactgc ggcaaccgac gagtccactc cgccggaaag
     1741 agcacagagt acttttttgt cgccgatctt ttcccgcaga tgggcaacca tgttgtccac
     1801 aaacgaggac atgtgccagg ttccagtaca gccgcataca ccgtacagga agtttcggat
     1861 catggtcatt ccttcagggg tgtgcagaac
//
LOCUS       DALUAJ010000226         1753 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_43842,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000226 DALUAJ010000000
VERSION     DALUAJ010000226.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43842"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="O0X37_04785"
     CDS             <1..602
                     /locus_tag="O0X37_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HJK62269.1"
                     /translation="ITPLLAGAGIAGVAVALAAQDVLGNIFGGAVLYVDTPFRVGDWV
                     KVSGQFGEVLQIGPRSTRIKTLDAQLMTIPNSKIADDAVINFSAPQEYMLIRLKIGVA
                     YGSDVELVKKTMKDAVDNVFATTPYLSHDEPVEANFLSFGDSSLNFELVICAAKPLYY
                     YAAIDAVNCEVDRLFRERNISIPFPQREVRILNMPDHGL"
     gene            686..1423
                     /locus_tag="O0X37_04790"
     CDS             686..1423
                     /locus_tag="O0X37_04790"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HJK62270.1"
                     /translation="MKKSAVAVLVLFAVFALLTLPAAAENSQTDRVILTSGYGESVTT
                     PDKVMISFAVETTNPDAKIAQQQNADQMAAVVAALKSAGIAETDLKTTGYNIYAYTIS
                     EYNPGEWPNGTKVYQVRNTIQITSYDVDKAGEYIDAAVGAGANSVNSLQFGLSDAKQI
                     TERNKALVAAVAAARADADSVASALGVKIVSTGTVSVDQSRYAVSYNSVETAALKADA
                     ASGASVPTTIQSGELTTTATVSVVYTY"
     gene            complement(1430..>1753)
                     /locus_tag="O0X37_04795"
     CDS             complement(1430..>1753)
                     /locus_tag="O0X37_04795"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HJK62271.1"
                     /translation="LTAGGFCAAAVLSDGTSITAEDLSQPTAIDKACGSLLRTGHKPT
                     DAALIISGKPTADTIRKTLNAGFSILAGYLPPTVLAVKLADSGNLTIVEVPHKKIHTH
                     PERIR"
BASE COUNT          397 a          428 c          484 g          444 t
ORIGIN      
        1 acatcacccc gcttcttgca ggtgctggta ttgccggagt tgccgtggcc ctcgcggcgc
       61 aggatgttct tggcaacatc ttcggcggtg cggttctcta tgtggataca ccgttccgtg
      121 ttggtgactg ggtaaaggtg agtggtcagt ttggtgaagt actccaaatc ggtccgcgga
      181 gtacccgcat caaaacactg gacgcccaac tgatgaccat tcccaactcc aagatcgcag
      241 acgatgccgt gatcaacttc tccgcaccgc aggagtacat gctgattcgc ctaaagatcg
      301 gggttgccta cggttcggat gtggaactgg tgaaaaagac catgaaggat gctgtggaca
      361 acgtctttgc cacaactccg tacctttctc acgatgaacc ggtggaggca aacttcctgt
      421 cgttcggcga ctccagcctg aacttcgagc tggtaatctg tgcagcaaag ccgctgtatt
      481 attatgcagc aatcgatgca gtgaactgtg aggttgaccg gctgttccgt gaaagaaaca
      541 tctcgattcc gttcccccag cgggaggttc ggatcctcaa tatgccggat cacggcctct
      601 gactttcttt ttttaaaact acagaaattt tggtagttta ccatagctat atgaattctc
      661 aggagcaatt actccattga tacagatgaa aaagagtgca gtcgcagttc ttgtgctgtt
      721 tgcagtgttt gcccttctta cacttcctgc agcagcagag aactcccaaa cggaccgggt
      781 gatccttaca tccggttatg gtgagtcggt gacgactcca gataaggtta tgatcagttt
      841 tgctgttgaa acaaccaacc cggatgcaaa gattgcacag cagcagaatg cagatcagat
      901 ggcagcagtt gttgcggctc tgaaaagtgc aggtattgcg gaaactgatc tgaagacaac
      961 cggatacaat atttatgcat acaccatcag cgagtacaat ccgggtgagt ggccgaacgg
     1021 taccaaagtc tatcaggtca gaaacacgat tcagattaca tcctatgatg tggataaagc
     1081 cggcgagtac attgacgctg cggttggtgc cggagcaaac agtgtaaaca gcctccagtt
     1141 tggtctttcg gacgcaaaac agatcactga gcgtaacaag gctctggtcg cagcggttgc
     1201 cgctgcacgt gccgatgccg attccgttgc ttcggctctc ggtgtgaaga ttgtctccac
     1261 tggtaccgtg tcggtggatc agagcagata tgcggtctct tacaactcgg ttgagactgc
     1321 tgccctgaaa gctgatgctg catcgggagc aagcgttccc acaacgattc agtccggaga
     1381 gctgacgacc actgcaaccg tctcggttgt ttacacctac taactttttt tatctgatcc
     1441 gttccggatg agtgtggatc tttttgtgcg ggacctcaac gatagtgagg ttaccggagt
     1501 cagcgagttt tacggcaagc actgtcggcg gcagatagcc tgcgagaatg gagaaccctg
     1561 cgttgagcgt ttttcgtatg gtgtctgcgg tcggttttcc ggagatgatg agggcagcat
     1621 ctgttggttt gtgccctgtc ctgaggagac tgccgcatgc tttgtcgatt gcggtcggct
     1681 ggctgagatc ctcggcggtg atactggtgc cgtccgatag aactgctgct gcgcagaatc
     1741 ctccggctgt gag
//
LOCUS       DALUAJ010000227         3060 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_44317,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000227 DALUAJ010000000
VERSION     DALUAJ010000227.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44317"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /gene="rqcH"
                     /locus_tag="O0X37_04800"
     CDS             <1..1188
                     /gene="rqcH"
                     /locus_tag="O0X37_04800"
                     /inference="COORDINATES: protein motif:HMM:NF041120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue protein RqcH"
                     /protein_id="HJK62272.1"
                     /translation="DEKGAFPLPSVLREPKEHYPTFSAALEAWFPKPVAESVVEAKVK
                     LSREERIRKQQEEALVKFERKISEAMESSEIIYSHYGEVQETINVLSQASERMSWQDI
                     AKVLKGSDMPAARRIVSVNPADASVVLDLDEKHRVTIFVHESLEANVGRYYQVAKKFR
                     AKKEGALRAMERAIVHPAKPKSHGPGKMKAKWYHRFRWMETSDGVVVIGGRNADQNEE
                     LVKKYMEGKDTFLHADVFGASVVIVKGLTEQMDEAVQFAASYSRLWGSGAAAGDVIAA
                     APSQVSKTAESGEFVAHGSFVIRGERKIFRNVPMEVAIGVQMEPVVAVLGGTPSAIEP
                     RCQVAVRLRPGTFEGNDVAKKVLRRLKEGLPESDQKLLKAVLNTEAVAAFVPPGGSDL
                     VES"
     gene            1185..2219
                     /locus_tag="O0X37_04805"
     CDS             1185..2219
                     /locus_tag="O0X37_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA surveillance protein pelota"
                     /protein_id="HJK62273.1"
                     /translation="MKATVAEPLRRDGFGEYKLVPESLDDLWHLSHLISWGDTVFAVT
                     LRTVDGPNDKLRAEKLEKRPVRIGVKCEKVEFLPAVGRLRVFGVIVFGPDTGQHHTLN
                     VETGYEISVVRQWRQVDLERLERAVAASVHGVVHIVAVEDGEAEVYRVRQYGPERVVT
                     LTIGSGKTAEIDSRQSLFEELLRALAMVTGPVVVAGPGFVKEDFVKFARSSVPETAER
                     MLLADTRRSGYGAVQEAIGNGVLTRIAEDLQLAREVQVMDEVFLRIGQNGAVAYGPAE
                     VREAIDYGAAETVIVADALVRENRTSKMIEDAERLGAGVVVLSTEFEPGKRLAGLGGV
                     AALLRYKIAT"
     gene            complement(2320..2889)
                     /locus_tag="O0X37_04810"
     CDS             complement(2320..2889)
                     /locus_tag="O0X37_04810"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HJK62274.1"
                     /translation="MIIVGVGAGSGMLTEDGISRIRTARVIYGSGRALDLARGYAAAD
                     AKLILITDYKALRSLPDDAVVLSTGDPLMAGLGYLPGEVVPGISSMQVAFARLKVPWT
                     NVAVVNAHGKDHTAAVERAVRDVAAGHVVFIIADPDFSLAALGSALSPDVRIAVCEDL
                     GYPSERVAEGTAACPPEVRSRLFCVVAGY"
BASE COUNT          801 a          692 c          923 g          644 t
ORIGIN      
        1 gatgagaaag gagcatttcc gctgccgtct gtcctgcgag agccaaagga acattatcct
       61 acattttcag cggctcttga agcatggttc ccaaaacctg ttgcggagtc tgtggttgag
      121 gcgaaagtga aactgtctcg ggaggaacgt atccgtaaac agcaggagga agcactcgta
      181 aagtttgagc ggaaaatttc cgaggcgatg gagagctcgg agataattta ttcgcattac
      241 ggcgaggtgc aggagacgat caacgtactc tctcaggctt cagaacggat gtcttggcag
      301 gatattgcaa aagtcctaaa aggatctgat atgcctgcag cacggcggat cgtatcagtg
      361 aatccggcag atgcatcagt tgtgctggac ttagacgaga agcaccgagt gacgattttt
      421 gtgcacgaaa gtcttgaggc aaatgtaggg cggtactatc aggtggcaaa aaagttccgt
      481 gcaaaaaagg agggagcact tcgggcaatg gagcgggcga ttgtacatcc agcaaaaccg
      541 aagtcacatg gtcccggaaa gatgaaggca aaatggtatc atcggttccg gtggatggag
      601 acatctgacg gtgtggttgt tatcggaggt agaaatgcgg atcagaatga ggagctggta
      661 aagaagtaca tggaaggaaa ggacacgttc ctgcacgcag atgtctttgg agcatcagtg
      721 gtgatcgtga aagggttaac agaacagatg gatgaagctg tgcagtttgc tgcgtcatac
      781 tcgcgtttat ggggatcggg tgctgcagca ggcgatgtga ttgcagcagc gccgtcccag
      841 gtaagtaaaa ctgcagagtc aggtgagttt gtggctcacg gatcatttgt gattcgcggg
      901 gaacggaaga ttttccggaa cgtaccgatg gaggtcgcaa taggggtcca gatggagcct
      961 gttgttgcgg ttctcggcgg aacaccgtct gcgatcgagc cccggtgcca agtggcggtt
     1021 cgcctgcggc caggtacttt tgaaggaaat gatgtagcaa agaaggtact gcggcggctg
     1081 aaagaggggc ttcccgagtc ggatcaaaag ttgctgaaag ctgtcctgaa tactgaggcc
     1141 gtggctgcat ttgtgccgcc gggcggatct gatctggtgg agtcatgaag gcgacggtag
     1201 ctgagccgct gagacgtgac gggtttggcg agtataagct ggtaccggaa agcctggatg
     1261 atctgtggca cttatcacat ctgatttcgt ggggagatac tgtttttgca gtaacactgc
     1321 gaacggtgga cgggccaaat gacaaacttc gggcagaaaa actggagaag cgacctgtac
     1381 gcataggggt aaagtgtgag aaggtagaat tcctgcctgc agtcgggagg cttcgggtat
     1441 tcggcgtgat tgtattcgga ccggatacag ggcagcatca cacgctgaat gtggagacag
     1501 gatatgagat cagtgtggtt cgccagtggc ggcaggtgga tctggagcgg ctggaacgcg
     1561 cagtagcagc gtctgtacat ggtgtggtac atattgtggc cgtggaggac ggggaagcag
     1621 aggtgtatcg tgttcgacag tacgggccag aacgggtagt tacactgacg attggcagtg
     1681 ggaagacagc agagatcgac tcaaggcaga gtttgttcga agaactgcta agagcacttg
     1741 caatggtgac tggaccggtg gtggttgcag gtccgggatt tgtgaaggag gattttgtga
     1801 agtttgcacg ttcgtcagta ccggaaactg cagaacgtat gttacttgcg gatacgcggc
     1861 gatcgggcta tggtgcagta caggaagcga ttgggaatgg cgttctgaca cggattgccg
     1921 aggatctgca gctggcacgg gaggtacagg taatggatga ggtgtttttg cggatcggac
     1981 aaaatggtgc ggtggcatat ggtcctgcgg aggtacggga ggcaatagat tacggtgcgg
     2041 ctgagacagt gattgtggca gatgcactgg tacgggagaa cagaacatcc aagatgattg
     2101 aagatgcaga gcggcttggg gcaggagtcg tagtgctttc aactgagttt gagccgggga
     2161 aacgtctggc aggtctgggc ggagttgctg cactgcttcg gtataaaatc gcaacatagg
     2221 tgtaagtgag ataaagaatt tttttatttt acaggtactt ggtgcgaata ctatcaaaga
     2281 atgaaaaaat cgtaccgcac aaaacaagaa aaagaaaaat cagtaccctg caaccacaca
     2341 aaacaacctg cttcggacct ccggcggaca tgcggctgtc ccctccgcaa cacgctcgga
     2401 cgggtaccca agatcttcac agaccgcaat ccgtacatcc ggagagagcg cggacccaag
     2461 tgccgcaaga gaaaaatcag gatccgcaat tataaacaca acatggcccg cagccacatc
     2521 ccgcaccgca cgttccacag cagcggtatg atcttttcca tgggcattca ccaccgcaac
     2581 atttgtccac ggaaccttca gcctggcaaa tgccacctgc attgacgaaa ttccaggcac
     2641 tacttctcca ggaagatatc caagtcccgc catcagggga tcacccgttg ataacacaac
     2701 tgcgtcatca ggaagagatc gcaacgcctt ataatcggta atcaaaatca gctttgcatc
     2761 cgccgctgca tatcctctgg ccagatcaag cgcacggccc gaaccgtaga taacccgtgc
     2821 agtacgaatg cgggaaattc cgtcttcagt cagcatcccg gaaccagcac caacaccgac
     2881 aatgatcatc ttacaccctc ctcggaatca accagtacct ctccatcacg atccagcagt
     2941 acgatacgca cccctggaaa ccgctgcacc gcatctgtca cctcggcctt taatacagcc
     3001 ggaccctcag cggaagccgc cagctcctcc accgttgaaa aaccccggga cgttgcaacc
//
LOCUS       DALUAJ010000228         3675 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_44350,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000228 DALUAJ010000000
VERSION     DALUAJ010000228.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44350"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="O0X37_04815"
     CDS             <1..236
                     /locus_tag="O0X37_04815"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HJK62275.1"
                     /translation="GSFNGQAPVNLYFGSYTVTEDGLTFGAVGSTMMAGPEEDMAAES
                     EFYSALGSVAGYKVVDGQLLLTDAAGNTVLTFE"
     gene            complement(319..969)
                     /locus_tag="O0X37_04820"
     CDS             complement(319..969)
                     /locus_tag="O0X37_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62276.1"
                     /translation="MSELSQLRTENNELEKKLTKENSDLITRMYFYLLSSNLKELEVE
                     TIRKDLTGMALEAQQREEQFSDAVGEDYKQFCDELIANGRTKSIPAKILEVIELCLYA
                     GVVLLIVEMITAGSLDLASTLTLTPAFFTLILFAVVCGTIILWILSRAVWGLPEKDRW
                     KNRCIAGTAAGIILIGIPLFTVILPKTELITIHPWPVLAAAVILLAAVTLIRKRVE"
     gene            complement(971..1297)
                     /locus_tag="O0X37_04825"
     CDS             complement(971..1297)
                     /locus_tag="O0X37_04825"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HJK62277.1"
                     /translation="MFDRTQLMKGTLEGCILLIIGRETTYGYEIMEKLIQYGFCGIRE
                     GTIYPLLVRLEKKNLITSEYRVSPLGPSRKYYHLTDSGRKTAEEFAEAWQDISHAVER
                     ILAEEE"
     gene            complement(1405..2262)
                     /gene="speB"
                     /locus_tag="O0X37_04830"
     CDS             complement(1405..2262)
                     /gene="speB"
                     /locus_tag="O0X37_04830"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HJK62278.1"
                     /translation="MQSFSNTLFADAETEYNDARYVIFGVPFDGTTSFKAGARDAPLA
                     VRAASYNLETYLPYYDIEMPEIGFHDMGDLYCDCLPDLVVDQVADAVSDLVKDEKIPV
                     MIGGEHSVTIGAVQTLAPAWYVVCDAHLDMQDEFRGSPYNHDCVTARVAETVKNIILI
                     GPRSGTREEFSVARKEYHLYTADDVQERGITAILEEVSGLIGDETVYLSIDADAVDCC
                     LTPGLGTPEPFGLTPTDIRSVIRRIAQKNVVGFDYVEVLPNDTGQTAVVAAHLIREFI
                     AGHFCSRRE"
     gene            complement(2280..2660)
                     /locus_tag="O0X37_04835"
     CDS             complement(2280..2660)
                     /locus_tag="O0X37_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007315008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-5A"
                     /protein_id="HJK62279.1"
                     /translation="MKEQTEVGKLKEGRYVVVDDEPCKIQSIAISKPGKHGAAKARID
                     VVGLFDGQKRSVVSPTSATIYAPIVERKTGQILTIAGNVVTFMDMKDFNNFELVVDDD
                     IVATFQPAQEVPYIESLGKRKLDI"
     gene            2782..3573
                     /locus_tag="O0X37_04840"
     CDS             2782..3573
                     /locus_tag="O0X37_04840"
                     /EC_number="3.1.3.11"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     fructose-bisphosphatase/inositol-phosphate phosphatase"
                     /protein_id="HJK62280.1"
                     /translation="MVTPFQFLSVCDEVGKMMVQELSPLVGTAYGGEELCIGADNTPT
                     ERIDRVAEDLVLSVFREKKICRSLLSEEAGMVDIGGDFGTAYLDPVDGTFNAVSGIPF
                     YALSIGLSDGKQMVAGYVKNLANGETFTAILGEGAYLDGRPIQVSTETMLDHSAMSVY
                     AKKFDVTRMMQLGHKIRRWRLLGASALELCYVACGRLDGFVDLRNTLRVTDAAAGILI
                     CREAGGRVTLPDGSPVEFPDNVAAGRCLVATNRVIHRKVIEYLRS"
BASE COUNT          799 a          885 c         1010 g          981 t
ORIGIN      
        1 acggttcctt caacggtcag gctccggtta acctctactt cggcagctac actgtcactg
       61 aggacggcct tacctttggc gcagttggtt ccaccatgat ggcaggacct gaagaggata
      121 tggcagcaga gagtgagttc tactcagcac ttggaagcgt ggcaggatac aaagttgttg
      181 acggtcagct tctcctcacc gacgcagcag gaaacaccgt gctgacgttt gagtgaattc
      241 aataatactg tcacaataca caccccaaac acacaccaat ttttcttttt tgaaaatttt
      301 ctaaaaacgt gagttggttt attctactct cttccggatg agcgttactg cggcaagcag
      361 aattactgcg gctgccagaa ccggccaggg atgaattgtt atgagttctg ttttcgggag
      421 aataacggta aacagcggta tgccgatgag tatgatacct gcggcagttc ccgcaataca
      481 gcggtttttc catctgtcct tttccgggag tccccatact gcccgggaga gtatccagag
      541 gatgatggtg ccgcagacga cggcaaacag gatcagtgta aaaaacgcag gcgttagtgt
      601 gagggttgag gcaagatcaa ggcttcctgc tgtgatcatc tccacaatca gcagaacaac
      661 acctgcataa agacatagtt caatgacctc cagtattttt gccgggatgg attttgttct
      721 tccgtttgcg atcagttcgt cgcagaactg cttgtagtcc tccccgaccg catctgagaa
      781 ttgctcctca cgttgttgtg cctctaatgc catgccggtc aggtctttcc tgatggtttc
      841 gacttcaagt tctttgaggt ttgagctgag cagatagaag tacatccgtg tgatcaggtc
      901 gctgttctct tttgtcagtt tcttttccag ttcgttgttc tcggttctga gttgtgataa
      961 ttccgacatg ttactcctcc tctgcaagta tgcgttcaac tgcatgagat atgtcctgcc
     1021 atgcctctgc aaactcttca gcggtttttc ggccgctgtc ggtgagatga tagtattttc
     1081 tgctcggacc aagcggggat acccggtact cggaggtgat caggttcttc ttttccagcc
     1141 ggaccagaag gggatagatg gttccttccc gtatgccgca gaagccgtac tggatcagct
     1201 tttccatgat ttcgtatcca taggtggttt ccctgccaat gatcagaaga atacatcctt
     1261 cgagtgttcc tttcatcagc tgcgttcgat caaacatttt ccaaccagtc tatttggtat
     1321 tacatgctac tttgctatac atagtagtat tcagtctttt cataaatacg tatcggaggt
     1381 actgaaaaaa agagaggtcc cgtcttattc gcggcgggaa cagaaatgac cggcaataaa
     1441 ctcgcggatc agatgtgcgg cgacgactgc ggtctggcct gtatcgtttg gcagaacctc
     1501 aacatagtca aatcctacca cgttcttttg tgcaatcctg cggatgactg atctgatgtc
     1561 cgtcggcgtc agcccgaacg gctccggcgt tccaagcccc ggcgtcaggc agcagtcaac
     1621 ggcgtctgca tcgatggaga ggtacaccgt ctcatcaccg ataagaccgc ttacctcctc
     1681 aagaattgcg gtgatgccgc gctcctgcac gtcatctgcg gtgtagaggt gatactcctt
     1741 ccgtgcgaca gaaaactcct cgcgggtacc gctgcggggt cctatcagga taatgttctt
     1801 gactgtttct gcaacccgtg cagtgacaca gtcatgattg tagggggagc cacggaactc
     1861 atcctgcata tccagatgtg cgtcgcagac gacataccag gcgggggcga gcgtttggac
     1921 tgccccgatt gttaccgagt gttcgccgcc gatcatcacc gggatttttt catccttgac
     1981 gagatctgat actgcatccg ccacctgatc taccaccagg tccggcagac agtcgcagta
     2041 gaggtcgccc atgtcgtgga acccaatctc cggcatctcg atgtcgtagt acggcagata
     2101 cgtctcaaga ttgtaggatg cggcacggac tgccagcgga gcatcacgcg cacctgcctt
     2161 aaacgaggtt gtcccatcaa aaggaacgcc gaaaataacg tagcgtgcgt cgttgtattc
     2221 agtttctgca tccgcgaaaa gagtgttaga aaaagactgc atggatgtat tgtgcagtct
     2281 tagatgtcaa gcttgcgctt gccaagggat tcaatgtagg gaacttcctg agcaggctgg
     2341 aaggttgcaa cgatatcgtc gtcaacaaca agttcaaagt tgttgaagtc cttcatgtcc
     2401 ataaaggtca caacgtttcc tgcgatggtc aggatctgtc cggtcttgcg ctcaacgatc
     2461 ggtgcgtaga tggttgcgga tgtcggggat acgacggagc gcttctgacc gtcaaagagg
     2521 ccaactacat cgattcttgc ctttgctgct ccgtgctttc ccggcttgga gattgcaatg
     2581 ctctggatct tgcacggctc gtcgtcaaca acaacgtaac ggccttcctt gagcttacca
     2641 acttctgtct gttccttcat gataattaac tctgactaat tatgtgaggt aattgcttat
     2701 gagtgttgcg acagatgcgt ggatgcccgt taattcaccc gtttatttca tgaggctgcc
     2761 gcactaatac aaatgtatcg tatggtaacg ccgtttcagt ttctttccgt atgcgatgag
     2821 gtcggaaaaa tgatggtgca ggagctttcc ccgctggtag gtactgccta tggaggtgag
     2881 gagttgtgca tcggtgcgga taatactccg acagaacgca tcgaccgcgt ggcagaggat
     2941 ctggttctgt cggtgtttcg tgagaagaag atctgccggt cgctcctttc ggaggaggcg
     3001 ggtatggttg atatcggcgg ggattttggg acggcctatc ttgatcctgt ggacgggaca
     3061 tttaatgcgg tctctggaat tcctttctat gcgttatcca ttgggctttc cgacggcaag
     3121 cagatggttg caggatatgt gaaaaatctt gccaacggag agacgtttac ggccattctt
     3181 ggcgaaggag cctatctgga cggcaggccg attcaggtat cgaccgagac gatgctggat
     3241 cattcggcga tgagtgtgta tgcaaagaag tttgatgtga cgcggatgat gcagctcggc
     3301 cataaaatcc gccggtggcg tctgctgggg gcgtctgcac ttgagctctg ttatgttgcc
     3361 tgcgggcggc tggacgggtt tgtggatctg cgcaacacgc tgcgggtaac cgatgctgcg
     3421 gcgggtattc tgatctgccg tgaggccggc gggcgtgtga cgcttccaga cggcagtcct
     3481 gttgagtttc cggataatgt ggcggcaggc agatgtctgg ttgcgacgaa ccgtgttatt
     3541 caccgcaagg tgattgagta tctgaggtca tgatgaaggt ctgtattgtt tccagagtgg
     3601 atttccggga gccggttgag actgcccagt cgcttggctg gatgctggct gatgcggggc
     3661 atgaggtggt ctatg
//
LOCUS       DALUAJ010000229         2144 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_44440,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000229 DALUAJ010000000
VERSION     DALUAJ010000229.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44440"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="O0X37_04845"
     CDS             complement(<1..505)
                     /locus_tag="O0X37_04845"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HJK62281.1"
                     /translation="MTDSTPPSSADAAKSPNKKTLGILSLAMINVAAVLSLRNFPTMA
                     MEGWNMIFWYALFTVCFLIPTALVAAELASTWPKSGGIYSWVKEAYPKDGSFITIWCS
                     WVNNLVWFPTVVSFFAATIAYVLLAPLLSDDPVFMVVVMLASFWAVTIFNFFGSRASN
                     LLATVGTA"
     gene            complement(706..2082)
                     /locus_tag="O0X37_04850"
     CDS             complement(706..2082)
                     /locus_tag="O0X37_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HJK62282.1"
                     /translation="MAHREVNRAEQNILAEMRTYFASGATRTLSVRKAALVKLRETIL
                     QYEAEILHALTSDLGRPAFETYAFEIAPVLQEIETLEKNLDSWTKPKKVKTPLLLFSA
                     KTEVRIEPYGLVLVIAPWNYPFHLLMMPVAGAVACGNVVAVKPSRRAPKTMRIVQTIL
                     SDVFPDVWVSVFPQISLEETYDYIFFTGGIETGRKIAVAAAQHLTPITLELGGKNPCI
                     VDETANLNVAARRIAWGKCVNAGQTCVAPDYLLVHDSVRDILVNKIAAEITAFYGENP
                     ATHPDYGKIITAEEYERLLNYCVSERILKQCGTHNPRERKLAPTLLAATCQDPITRRE
                     IFGPILPVITWNTISDLDKLLSPTPLALYLFTEDTAFAEHLISLHPSGGVCINDCMIQ
                     VANNNAPFGGVGTSGMGCYHAQHSIETFSRKRTVITRKYSPDPSLRYPPYSKSVLENI
                     RKLRRILF"
BASE COUNT          557 a          452 c          539 g          596 t
ORIGIN      
        1 atgcagtacc gactgttgcc agcagattgg atgctcggga accaaagaag ttaaagatgg
       61 ttactgccca gaagctggcc agcataacta ctaccataaa gactggatca tctgaaagca
      121 gcggagcaag cagaacgtag gcaattgttg cggcaaagaa ggagacgacc gttgggaacc
      181 agacaagatt gttcacccag gagcaccaga ttgtaatgaa gcttccgtcc ttagggtagg
      241 cttcctttac ccaagagtag atacctcctg attttggcca ggttgatgca agttctgctg
      301 caactaatgc ggttgggatc aggaaacaga cggtgaacag agcataccag aagatcatgt
      361 tccatccttc cattgccatg gtcgggaaat tgcgcaaact taaaactgca gcaacgttaa
      421 tcatggcaag ggagagaatg ccaagcgttt ttttgtttgg cgatttagcc gcgtcagcgg
      481 atgacggggg agttgaatca gtcatttgtg tcacctattt tcgttggtta caattgatcg
      541 cacagacacg gtaacagatg atttcaactc tccaaaagag actgtttctg aaatacatca
      601 gtcccatatc cggtcagtca cataccaagt atcgaggtgt atttaaataa gtgtatagat
      661 ggtgacaaaa ataaaaaaat tcagaataac tataatataa ttacctcaga agagtatgcg
      721 ccgcaatttc cggatgtttt ctagaacaga ctttgaatat ggagggtaac gaagtgaggg
      781 atctggagag tatttccgcg tgatgacagt tcgtttccgg gagaatgttt caatgctgtg
      841 ctgagcgtga taacatccca ttccgcttgt accaactcct ccaaatgggg cgttgttatt
      901 tgccacctgt atcatacagt cgttgataca tactcctcca gatggatgaa gagagatcag
      961 gtgttctgca aatgcggtgt cctcggtaaa gagataaaga gcaagcggtg ttggagatag
     1021 cagtttatca aggtcagaga ttgtattcca ggtaattacg ggaagtatcg ggccaaatat
     1081 ttctctgcgg gttattggat cttggcatgt tgccgcaagg agagtcggag caagctttct
     1141 ttctctggga ttgtgtgttc cgcattgttt gagaatgcgt tccgatacac agtagttaag
     1201 taaacgttca tactcctccg ctgttatgat ttttccatag tccggatgtg ttgctggatt
     1261 ttctccatag aatgcggtga tctctgctgc aattttgttc acgagtatgt cacgaacaga
     1321 atcatgtacg agtagatagt ccggggcaac acaggtctgt cctgcattta cgcatttccc
     1381 ccaggctatt cggcgtgctg caacgtttag gtttgccgtt tcatcaacaa tgcagggatt
     1441 ctttccccca agctcaagtg ttatgggtgt cagatgctgt gcggctgcaa ctgcaatctt
     1501 ccttccggtt tcgataccac cggtaaagaa gatgtaatca tacgtttctt caaggctgat
     1561 ttggggaaag accgataccc atacatcggg aaacacgtcg gaaaggattg tctgtactat
     1621 ccgcatggtc ttcggagcac gccgcgacgg ttttacggcg acaacgtttc cacaggcaac
     1681 tgcacctgca accggcatca taagaaggtg aaacggataa ttccaagggg caatgaccag
     1741 tacaagacca tagggttcaa tgcgtacttc tgtttttgca gagaagagga ggagcggggt
     1801 ttttaccttc tttggtttgg tccaggagtc cagatttttt tcaagtgttt caatttcctg
     1861 taatactggt gctatttcaa aggcatatgt ctcaaacgcc ggtctgccga gatctgaggt
     1921 gagtgcatgc agaatctctg cttcatactg caggattgtt tccctgagtt ttacaagtgc
     1981 ggcttttcgt acagaaagtg tccgagttgc accgcttgca aaataggttc gcatctctgc
     2041 gaggatattc tgttctgctc tgttcacctc tctgtgtgcc atgggtgaac attgggcgcg
     2101 tggctataaa tcctctctgg cggagtcctt tttagatcct gata
//
LOCUS       DALUAJ010000230         2483 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_44785,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000230 DALUAJ010000000
VERSION     DALUAJ010000230.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44785"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="O0X37_04855"
     CDS             complement(<1..1201)
                     /locus_tag="O0X37_04855"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HJK62283.1"
                     /translation="MNKLHLVLVLLVAVVMLTVPAAAEVALGGSVGGSTATISVTSSP
                     SGASVYDAGVYQGTTPCNIIVHTTGTPIDHDIVISKNGYYDYHQYIGTVVTDQYITVN
                     AVLTPVVQTGYLDVSSSPSGANVYIDGIYKGTAPLVVSVDAGSHSVRLEKPGYNTWYG
                     TYSVSAGKTVQVAASLSASVTYGYINVHSYPSAADVYIDGNYRDDTPAVITVTAGTAH
                     EVKVVHSGYEVYRQTVTVSSGSTVSIDANLVASGDAYIQLASYPAGAAVYVDGSYCGN
                     TGYSSSSSVNYMHIGPLSAGTHSIMLKKDGYNTYTSTVTLSPNEVRTLSVTLTPTSPT
                     PSGYSSLSLSSTPSGADVYVDNVFRGYTPIYLSDIPAGQHTLLLQHTGYNDWTEYVSF
                     TSDYTVEK"
     gene            complement(1298..1561)
                     /locus_tag="O0X37_04860"
     CDS             complement(1298..1561)
                     /locus_tag="O0X37_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62284.1"
                     /translation="MQIEYLSPGHALITDAGVSVDAYCLELGSANLIFVKTSSGIVGC
                     GFLISPLFKNSAFLPQKLLGSRPLRIFLRDLFPQLPLQQFSGG"
     gene            1652..2143
                     /locus_tag="O0X37_04865"
     CDS             1652..2143
                     /locus_tag="O0X37_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HJK62285.1"
                     /translation="MIREIRLYGDPVLTKSAETVPEITEEIRDILTDMWDTMEATRCV
                     GLSAPQIGVSLRLFVINAGGVVIRGANPEVLTEGAPSEDIEGSPCIPGIQKPVRRPRK
                     ITVRYLDESGEIVERELKGVTARAYLHEKDHHEGVLFIDHLKPGVRKLIQIDLDRIAG
                     NRA"
     gene            2193..>2483
                     /locus_tag="O0X37_04870"
     CDS             2193..>2483
                     /locus_tag="O0X37_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin:thioredoxin reductase"
                     /protein_id="HJK62286.1"
                     /translation="MTTPPTPEEIAALAPKIRADSEKLAQRKGWKLNPDTAIADSVIA
                     GLARNKLMRGKRYCPCRLPSGNPDVDKLFICPCRDAENDIETEGHCHCFLYMK"
BASE COUNT          714 a          583 c          624 g          562 t
ORIGIN      
        1 ccttctccac tgtataatca gaggtgaagg agacatactc tgtccagtca ttgtatccgg
       61 tatgctggag tagtaaagta tgttggcctg cggggatatc cgaaagataa atcggtgtgt
      121 atccgcggaa gacattgtcc acatacacgt cagcacctga tggtgttgag gaaagcgaca
      181 gactggaata accagacggt gttggtgagg ttggtgtcaa tgttacactc agggtacgga
      241 cttcgtttgg agacagtgta actgttgagg tataggtgtt atatccgtct ttcttcagca
      301 taatcgagtg ggtgcctgct gacaacggac cgatatgcat gtaattcaca gaagatgacg
      361 acgaatatcc agtgtttcca cagtaggaac catcaacata cactgccgca ccagctggat
      421 atgatgcaag ctgtatgtaa gcatctccgc ttgctaccag gttggcatca attgagacag
      481 tactaccaga actgaccgta acggtctgtc tgtacacctc gtatccggag tgaaccactt
      541 tcacttcatg tgcagtaccg gcagttacag taatcacagc cggcgtatca tcacggtaat
      601 ttccgtcaat gtacacatct gctgcggatg gatacgagtg gacattgatg tatccgtacg
      661 tcacagatgc tgaaagggaa gctgcgacct gtactgtctt tccggcggaa acactgtacg
      721 ttccatacca ggtgttatat ccaggttttt ccagccggac agagtgactt ccggcatcaa
      781 cagataccac cagcggagca gttcctttat agataccgtc aatgtacacg tttgcacctg
      841 aaggagatga cgagacatca agataaccag tctgtacaac tggtgttaat actgcattca
      901 ccgtgatgta ctgatcagtc acaacagtac cgatgtactg atgataatca tagtatccat
      961 tcttcgagat tacgatatca tggtcgattg gtgtacctgt tgtgtgaacg ataatattac
     1021 acggcgtcgt accctgatag actccggcat catacacaga cgcacccgag ggcgaagagg
     1081 tcacactaat cgtcgcggtg gaaccgccta cagaaccacc gagggcgacc tcagctgccg
     1141 ctggtacagt cagcattaca actgcaacca gcagcaccag aacaagatga agtttgttca
     1201 tgataaaagt atcaacgttg cctgagataa aaaaagaatc ggtcagatta gctacggctt
     1261 agtattctga cagcctcctc accactcata cccgggttta cccccctgag aactgctgca
     1321 ggggtagctg cggaaacaga tcccttaaga agatcctcaa tggtcgagat cccagtaact
     1381 tttgcggcag gaatgccgaa tttttaaaaa gcggcgagat caagaagccg cagccgacga
     1441 ttcctgaaga tgtttttaca aagatcaggt tcgcagaccc aagttcaagg caataagcgt
     1501 caacagaaac accggcatca gtaatgaggg catgaccagg ggagagatac tcgatctgca
     1561 taccgagttc atttcacatt ctttcctaaa acagtttacc caaccatcag atacgcaaaa
     1621 ggtatgggcg tggtctgcac agatctataa tatgattcgg gagatacggc tgtacggtga
     1681 cccggtactg acaaaatcag cagaaacggt accggagata acagaggaga tacgggatat
     1741 cctgacagac atgtgggata cgatggaggc aacccggtgt gttggtcttt ctgcaccgca
     1801 gattggagtt tcacttcggc tgttcgtgat caacgcagga ggggttgtga tccgaggtgc
     1861 aaaccccgaa gtccttaccg agggagcacc cagcgaggac atagagggaa gtccctgtat
     1921 tcccggaatc cagaaaccgg tacggcgacc gcgaaagatc acggtgcggt acctggatga
     1981 aagtggtgag attgttgagc gtgaactaaa gggtgtcact gcacgggcat atctgcatga
     2041 aaaggatcat cacgaaggag tactctttat tgatcatctc aagcctggtg tccgaaaact
     2101 gatccagata gatctggatc gaatcgcagg caacagagca taaagtcccc aaaccaaaaa
     2161 cagatatcct ccaaaaggga aaaatagagg tcatgactac ccccccaaca ccggaagaga
     2221 ttgcagcact ggccccgaaa atacgtgcgg attccgaaaa actcgcgcaa agaaaaggat
     2281 ggaagctgaa tccagatacc gcaattgcgg attctgtcat tgccgggctt gcacgcaata
     2341 aactgatgcg gggaaaacga tactgtccat gcagacttcc aagcggcaac ccagatgttg
     2401 acaaactgtt tatttgtccc tgccgggatg cagagaatga tattgaaact gagggacact
     2461 gccactgctt cctctatatg aag
//
LOCUS       DALUAJ010000231         2694 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_45325,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000231 DALUAJ010000000
VERSION     DALUAJ010000231.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45325"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            16..969
                     /locus_tag="O0X37_04875"
     CDS             16..969
                     /locus_tag="O0X37_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HJK62287.1"
                     /translation="MNLLVELSGENPVLATAEIDCVGTVTRTATGIALAEVPEPETTT
                     RLAQTHVVMELIGECDGTSAALAELLAQLDITADRPFACRVRKIHPATVDASQLDLER
                     MMGKSIHGTVSLQSPEVVYRALFTDNRCFLGRVLYTIDRGSYAYRNPQRRAFFHPGVM
                     MPLMARTMVNLTHVRPGELLCDPFCGTGGMLLETELMGVRSIGSDYDPEMLAGCRKNL
                     PDGAYVRADATRMPYPDNCFDAVATDLPYGQSTTIGADSLDYLYVESLKEIRRILKQG
                     GRAVVVTHRDIRHLAENLFEISGYYEQRVHKSLTRRILVLS"
     gene            1014..2150
                     /locus_tag="O0X37_04880"
     CDS             1014..2150
                     /locus_tag="O0X37_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62288.1"
                     /translation="MLRWISEQFTRYYPDRAIAVRAMRHMRPAERKNLFSEDIMLMRT
                     AEGYWFESLIYEQLLKIASQTNTIKKIVRKGADVPKHKTKITLGTNGLYSAEKGDIRV
                     RGNGQDLAEVDLLLFDNENKVVFCEIVTSPADLKDLEAEIIYKKHLFGYMFGQDHVGF
                     ILFSAVDISNTQVVRRLIADPDNAYVETKPCEVMKAVLRPLRLTTLPTQPVKNPKLVA
                     CESLKTRGFDYKKLHDEALSRLYFAIEHHFSAEQYFSDPCVSPIVKKVICGGLYPSAL
                     KGLVSEYGLRVKSEVLDYARLSQNYSKVVLAVDFPEMSPVIYLKPRKQRTYFKMVPLK
                     AGGFKYERPTPSRVGFYLWLESVKPTLGIERTREILKYCDHAKK"
     gene            2196..2381
                     /locus_tag="O0X37_04885"
     CDS             2196..2381
                     /locus_tag="O0X37_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62289.1"
                     /translation="MTEELDSDTIQHELKRLEGMILILEKENAELRDRISSLELQTGQ
                     LTDRILRLGGNCSSCGV"
BASE COUNT          721 a          670 c          718 g          585 t
ORIGIN      
        1 ctatctatag ggcggatgaa cctgttggtt gaactctccg gagaaaatcc ggtactggct
       61 acagccgaga tagattgcgt gggaactgta acacgcacag cgaccggtat cgctcttgcc
      121 gaggttcccg aaccggagac gacgacacgg ctcgcacaga cccatgtggt catggaactg
      181 atcggcgaat gtgacggaac cagtgccgca cttgcagaac tgctggcaca attagatata
      241 accgcggacc gcccctttgc ctgcagagtc cgaaagattc atcctgcaac ggttgatgca
      301 tcccagctgg acctggaacg catgatggga aaaagtattc acggaacagt ttcccttcag
      361 tccccggaag tggtgtatcg tgcgctcttt acggacaacc gttgctttct cggccgggta
      421 ttatacacaa tcgaccgagg atcctatgca taccggaacc cgcagagacg ggcattcttc
      481 caccccggcg tgatgatgcc gctgatggca cggacgatgg taaacctgac gcacgtacgg
      541 cccggtgaac tgttgtgcga tcctttctgt gggactggtg gtatgctgct tgaaacggaa
      601 ctgatgggcg ttcgatccat cggaagcgat tacgatccgg agatgcttgc gggatgccgg
      661 aaaaacctcc cggacggtgc atatgtccgg gcagatgcaa cacgcatgcc ctaccctgac
      721 aactgctttg acgcagtggc aacggatctc ccatacgggc agtcaacaac aatcggtgca
      781 gattcgctgg attatctata tgttgagtcg ctcaaagaga ttcgccgcat actaaaacag
      841 gggggacggg cagtagtagt gacgcatcgt gacatacggc atcttgcaga gaacctcttt
      901 gagatctccg gatattacga gcagcgggtg cacaagagtc tgacgcggcg gattctcgtg
      961 ctgagctgaa aggtcagaac tgtagatatg acgaatatac aaaaggagct ttgatgctcc
     1021 ggtggattag tgagcagttt acccgctatt atccggacag ggcaatagcg gtacgggcga
     1081 tgcggcatat gcgcccggca gaacggaaga atctgttctc ggaagacatt atgctgatgc
     1141 ggacggctga agggtactgg tttgagtccc tgatttatga gcagctgtta aagatcgcat
     1201 cacagacaaa tacgatcaaa aagattgtga gaaagggtgc ggacgttcca aaacacaaaa
     1261 caaagatcac gcttggcaca aacgggctct actccgcgga aaaaggagac atccgggtcc
     1321 gcggaaacgg tcaggacctg gccgaggtgg atctgctgct gtttgataat gaaaataagg
     1381 tggtgttctg cgaaattgtg acgtcccctg ccgatctgaa agatctggag gcagaaatca
     1441 tctacaagaa acatctgttc gggtacatgt tcggacagga tcatgtggga ttcattctgt
     1501 tttccgcggt ggacatttca aacacacagg tcgtgcggcg gctaattgcg gatccggaca
     1561 acgcatatgt ggagacgaaa ccatgtgagg tgatgaaggc agtcctcaga ccgctccggc
     1621 tgacgacgct gcctacacag ccggtgaaaa atccgaaact tgtggcatgc gagtcgctaa
     1681 agaccagagg attcgattac aaaaaactgc atgatgaagc attgtcgcgt ctgtattttg
     1741 caattgagca tcacttttca gccgagcagt acttttcgga tccctgcgta tcgccgattg
     1801 tgaaaaaggt gatctgcggc ggtctctacc cgtcagcact gaaaggtctg gtctccgagt
     1861 atggcctgcg ggtaaagtcg gaggtcctgg actatgcaag actttcacag aactactcaa
     1921 aggttgtact tgcggtggac ttccccgaga tgtcaccggt tatttatctg aagccgagaa
     1981 aacagcggac atacttcaag atggtgccgc tgaaggcagg cggttttaag tatgagcgcc
     2041 cgaccccgtc acgggtaggg ttctatttgt ggctggaatc cgtaaagcct acactgggaa
     2101 tcgaacgaac acgggagatt ctgaaatact gcgatcatgc aaaaaaatag acccaatctc
     2161 cacatctata aactccctca gcaaaacaaa gatacatgac tgaagaactt gacagtgaca
     2221 caatccagca tgaactcaaa cgcctcgaag gaatgattct catcctcgaa aaagagaacg
     2281 ccgagctgcg ggaccggatc tcatcgctgg aactccagac tggacaactc acggaccgca
     2341 tactgcgcct tggcggcaac tgttcctcat gcggggtctg aaccgccaaa catcccgcag
     2401 tttccggatt ttgttgtccg tataccgtat ccccgagaag gaccaaaaga acaaaacctg
     2461 cggcaagaaa aacagagcgt tcaagatagt tcagcgacgt catgacattg aacccgcata
     2521 ctcagagtat gaagtttact tcataatcac cctcggccgg accggaaagt ccgggcacaa
     2581 aacagctcta tttttccgca catgtgtacc ttaccgagca tttttttacc cattacacgc
     2641 ataaaaaaca ccaccgacag gatcagctcc gggtattttc ctttgtaatg caag
//
LOCUS       DALUAJ010000232         3488 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_45462,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000232 DALUAJ010000000
VERSION     DALUAJ010000232.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3488)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3488)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3488
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45462"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(103..384)
                     /locus_tag="O0X37_04890"
     CDS             complement(103..384)
                     /locus_tag="O0X37_04890"
                     /inference="COORDINATES: protein motif:HMM:NF021397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2098 domain-containing protein"
                     /protein_id="HJK62290.1"
                     /translation="MSTPAIGQTVRYGRTGTVGKIVAFVEENGFTFAELDSTGLYYRV
                     DQLTAISEVAHKETRHRDFKKDLEEEQKRFREMNESAWQNTDQSCEGGG"
     gene            complement(480..3074)
                     /locus_tag="O0X37_04895"
     CDS             complement(480..3074)
                     /locus_tag="O0X37_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004576 - oligosaccharyl transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006487 - protein N-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharyl transferase, archaeosortase A
                     system-associated"
                     /protein_id="HJK62291.1"
                     /translation="MDLSFWNDRRYRYGILGGIVLILTVLAFWIRALPFPDLAGTGDM
                     IAGPDAWYNLRLIEVALANNFGYIHFEPMTLYPTGQDIVWGPLFTWIATAAVALAGAG
                     TRPEIIDAAGWIPALLGAVMVPVMYWFGSRIGNWKTGLISALFIAVIGGQYLSRSLYG
                     HLDHHIAETLFSTLFCLLYVVALYSLNDHKIDFKKYETLKLPVLYGVICGVSYLLGLL
                     TMSTMVVFGLFVAIFTLIQFIISHRSGKQMEYLLVLNVITFAVVAFGMLIYGIQDTSF
                     SFYSYSLGVFCAHLGIIVGTIVLYAVSKILSAKKMPWYYFPGILIIFSIIGIGIISIG
                     IPSLYNTAIGGLSNFFVQNPAASTIAEMAAWTMEGAVSSFGWGLLLAAGGFIYLLYRA
                     WKHEEPGALFVLIWSALMIFATMQHVRWEYYVAANIALLAAVFVGWAITFAEKDLQQM
                     IGSKKPEERDSTPTKKGKKATAKSSSGPDLLKVGTCIVVVLIALVFVGTSAATAVQTG
                     ESYGKYGGTEADWISECTWMLTGTPETGVDYLTIYDGDEFEYPSEAYGVMSWWDYGHY
                     ITTIAERIPNSNPFQAGVAGTYGAAAVLTSTNESAVMEKLDYLGTRYIMTDYQMAGAK
                     FGAMAIWNDSTAQLTPYYYTFLQNSNGNLQVVQAYTPEYYNTLTVKLQYYDGSMTEPG
                     DVAVVETDSSANYGYPVITNVQGYATAEEAQAAADAFNANAPATKHAYVIANPSGVAT
                     AYLPSTTVPALQHFRLVHESPNYVLANSQYTNQPVEGGTAWVKSFEYVQGAVINGEGT
                     IEVNVTTNNGRTFTYRQESIDGQFVVPYSTYGSSYDVKTTGPYTIVETGETFEVSEEA
                     VMQGLTIN"
     gene            complement(3160..>3488)
                     /locus_tag="O0X37_04900"
     CDS             complement(3160..>3488)
                     /locus_tag="O0X37_04900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011449801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJK62292.1"
                     /translation="SKYISDEDVPYFFSAANLLALPYTRASQSGVAHIGISYGMPIVA
                     SKVGGLAESLGQYEGTYFVSPENLAELSHALETVLTEDHEKYPIPEFMRWDHIATTWK
                     MLIDQV"
BASE COUNT          861 a          839 c          835 g          953 t
ORIGIN      
        1 ctgaaggaag ttgcagaaaa actgatggca ctcaagggtg ttgagtcggt caaactgacc
       61 actatcccgg taaactgatt tttcaaaaaa tgggaggaga gtttatcctc cgccttcaca
      121 actttgatcg gtattctgcc aggctgactc attcatctca cggaatctct tctgttcctc
      181 ctcaagatcc tttttgaagt cacggtgcct ggtctcttta tgggcaacct cactgattgc
      241 agtgagctgg tcaacacgat agtacagacc ggtactgtca agctctgcaa aggtgaatcc
      301 gttctcctca acgaatgcca cgatcttccc gactgttcct gttctgccgt accgcaccgt
      361 ctgcccgatt gcaggagtgc tcatgctact atgttggatg gtgccggagt ataaagatgt
      421 ggattggata tcttgttgtc tgaaaaaagt ggatctggta ggatagccag atgtccggtt
      481 tagttgatag taagaccctg catcacggcc tcttcagaga cctcgaatgt ctcacctgtc
      541 tctacaatgg tatagggtcc tgtcgtcttc acgtcgtatg aacttccgta ggttgagtac
      601 gggacaacga actgtccgtc aatgctttcc tgccggtaag tgaaggttct accgttgttg
      661 gtggtaacgt tgacttcaat ggtaccctct ccgttgatta cggcaccctg cacgtactca
      721 aaactcttga cccatgctgt tccgccttcc accggctggt ttgtgtactg actgttagcc
      781 agcacatagt tcggggactc gtggaccagg cggaagtgct gcagtgccgg aaccgttgta
      841 ctcggcagat atgcggtagc tacacctgac gggtttgcaa tcacataggc atgttttgtt
      901 gcaggtgcgt ttgcattgaa tgcgtcagct gctgcctgtg cttcttctgc tgttgcatat
      961 ccctgtacat tggtgataac tggataaccg tagtttgcgg atgagtctgt ctcaactact
     1021 gcaacatctc ccggttcggt catcgaccca tcgtagtact ggagtttgac ggtgagtgtg
     1081 ttgtagtact cgggtgtgta cgcctgtact acctggaggt ttccgttact gttttgcagg
     1141 aatgtgtagt aatacggtgt cagttgtgct gttgaatcat tccagattgc cattgcaccg
     1201 aacttagcac ctgccatctg gtagtcggtc atgatatagc gggttccgag gtagtccagt
     1261 ttttccatta ctgcggactc atttgtactg gtcagtacag ctgctgcacc gtaggttccg
     1321 gcaacgcctg cctggaatgg gttactgttt ggaatgcgtt cagcaattgt ggtaatatag
     1381 tgaccatagt cccaccatga cataacaccg taggcttcgg acgggtactc aaactcatcg
     1441 ccgtcataga ttgtgaggta gtcaactccc gtctccggtg ttcctgtgag catccaggta
     1501 cactccgaga tccagtctgc ttcggttccg ccgtacttcc cgtagctttc accggtctga
     1561 acagcggttg ctgctgatgt tccaacaaag acgagggcta tcagtaccac aacaatacag
     1621 gttccgactt taagcaagtc tggtcctgag gaagactttg ctgtcgcctt tttccctttc
     1681 tttgtaggtg tactgtcacg ttcctctggt tttttgcttc ctatcatctg ctgaagatct
     1741 ttttcagcaa aggtgatggc ccagccaaca aacactgcgg caagcagggc gatgtttgca
     1801 gccacgtagt actcccagcg tacgtgctgc atggtggcaa agatcatcag tgccgaccag
     1861 atcagaacaa agagtgcccc aggttcctca tgtttccatg cacggtagag taggtagatg
     1921 aaaccccctg cggctaacag gagaccccac ccaaaggaac tgaccgctcc ctccatagtc
     1981 cacgcagcca tctcggcgat tgtggatgca gctggattct gtacaaagaa atttgacaga
     2041 ccaccaatag cagtattgta caatgatggt attccaattg aaattatgcc gattccaatt
     2101 atggagaata ttatcaatat ccccggaaaa taataccacg gcatcttttt tgctgagaga
     2161 atctttgata ctgcgtaaag cacaattgta ccgactatta tcccaaggtg agcacaaaac
     2221 acacctagtg agtaagagta gaagctgaaa cttgtgtcct gaattccata tatcagcatg
     2281 ccaaaggcta caactgcaaa tgtaatcaca ttcagaacaa ggagatactc catttgtttt
     2341 cctgaacggt gactgataat gaactggatc agcgtaaata tcgcaacaaa cagtccgaaa
     2401 acaaccatcg tggacatcgt cagaagaccg agcagatagg aaactccgca gattacacca
     2461 tacaagaccg gtagcttcag tgtttcatac tttttgaagt ctattttatg atcgttgagg
     2521 gagtaaaggg ctacaacata caggagacag aacagtgttg agaacaatgt ttcggcgatg
     2581 tggtgatcca gatgtccgta aagggatcgt gatagatact gtccaccgat tactgcgata
     2641 aacagtgcgg aaatgagacc tgtcttccag ttgccgattc ttgaaccaaa ccaatacatg
     2701 accggaacca ttaccgcgcc cagcagtgca gggatccatc ctgctgcgtc gattatttcc
     2761 ggtcgtgttc cggctcccgc aagtgctaca gcagctgttg caatccaggt aaacaagggg
     2821 ccccagacaa tatcctgtcc tgttgggtac agggtcattg gctcaaaatg gatatatcca
     2881 aagttatttg caagtgcaac ttcaattaac cgcaggttgt accaggcatc tggccctgcg
     2941 atcatatctc cggttccggc gagatccgga aacggtaggg cgcgtatcca gaaagcaagt
     3001 accgtgagga tgagtacaat gcctccgagg attccgtaac gatatcggcg atcgttccaa
     3061 aaactcagat ccatatcttg atcacatcag atgtttgtaa tgctattgta tgccgtttta
     3121 tcacataagt tgattgcacg atcctgcggt aaaatgatgt tacacctgat cgatcagcat
     3181 cttccatgtt gtggcaatat gatcccatct catgaactca ggaatgggat acttctcatg
     3241 atcttctgtg agaacggttt ctaatgcgtg cgatagttct gccagatttt ctgggcttac
     3301 aaaatatgtc ccttcatact gacctagact ttctgcaagt cctcccactt ttgaggctac
     3361 gattggcatt ccataggata taccaatgtg agctacgcca ctttgtgatg cccgtgtgta
     3421 cgggagagct agcagatttg ctgccgaaaa aaagtatggg acgtcttcgt cactgatgta
     3481 ctttgaga
//
LOCUS       DALUAJ010000233         3525 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_45824,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000233 DALUAJ010000000
VERSION     DALUAJ010000233.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45824"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="O0X37_04905"
     CDS             <1..248
                     /locus_tag="O0X37_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK62293.1"
                     /translation="YAQCVESLCAGCGMCVGQCPYSALSMIINDEGRTVMSVTAAKCK
                     GCGTCGGFCPGGAIRMQHFTSPQILAQIDAFFLGGEQ"
     gene            248..682
                     /locus_tag="O0X37_04910"
     CDS             248..682
                     /locus_tag="O0X37_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HJK62294.1"
                     /translation="MADEWKPKIIGIICNWCSYAGADGAGSARTQYPPDVRIVRVMCT
                     GRIDTLFVLKAFADGADGVLVSGCHFGDCHYLAGNYKAAKRMFLVKSIMQNMGLEHRR
                     FRMTFVSASEGAKWAVVINDVINTVKEIGPSPIAAERKRRGL"
     gene            808..1257
                     /locus_tag="O0X37_04915"
     CDS             808..1257
                     /locus_tag="O0X37_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide-binding protein"
                     /protein_id="HJK62295.1"
                     /translation="MQKGDQRKYGNGIIVSVVTCTEIKQGIAIETGKTSPQYFNACSI
                     IELHEDDFKALGINKNTNVRVTSAAGSVILKAVVTTQSLYPGLAHIPMGPWANMVVPA
                     YTYSTGEPCFCGFDCLIEPAPEEKIENAVKLLHEKCGLKYVGDPHYD"
     gene            1250..2611
                     /locus_tag="O0X37_04920"
     CDS             1250..2611
                     /locus_tag="O0X37_04920"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit B"
                     /protein_id="HJK62296.1"
                     /translation="MTKTIKNVICTFCGTLCDDLEVDVTDDEKKIVTVRNACAIGAEK
                     YLHVSNPGRVVLPRMRQADGTYKDVSYDEAIDFAAKTLLKAKKPLFYGWSSTNCEAIA
                     AGNVLAEKTGGVIDNCATVCHGSSLLAIQDTGVPSCTLGEVKNRADRVIFWGCNPAHA
                     HPRHMSRYSIFPRGFFSAKGQKGRKIICVDCRETDTAKVADTFVRIKQSYDYEIFDAL
                     RTLLRGEDIPDEVGGVPKEKLQEVIDICKSGRFVTLFFGMGLTHTLGRNHNIDAGINF
                     IRDLNDYTKAVLIAMRGHYNVTGAGQVLGWQYGFPFACDLSRLTMARHNPGETTSVDL
                     LVRKEVDACVVIASDLAAHFPFEATRTMAHIPTITIDPHISMSTELSDLHIPVAIVGV
                     EVGGCCYRMDNVPIETRKCIDAPEGVLTDEELFNRISARVDEILAENGCKDASEYLAK
                     MEA"
     gene            2614..>3525
                     /locus_tag="O0X37_04925"
     CDS             2614..>3525
                     /locus_tag="O0X37_04925"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit A"
                     /protein_id="HJK62297.1"
                     /translation="MSEIIIKNGHVFDAVTGLKGDVADVCIKDGKFVDKVSNAATVID
                     ATGKTVMAGALDVHTHVAGPKVNLGRWYRPEDKFRLGERCSAKINPKTKIEGGFSIPT
                     VYKTGYEYARTGFGFLMEAAMPPLYSRHVHEEIRDTPIVDEAAMPVFGNNWFMFEYLK
                     NGEIDNAAAYVAWMLRQTKGYGIKCVNPGGSEAWAWGLNCMTINDPVPYFEITPKEII
                     AGLITTNEELHLPHSVHLHSNNLGNPGNYTTTLDSLKIAEDFKPNNNLGRKTVLHHTH
                     IQFHSYGGTGWGDFESKSKEGMDYGKST"
BASE COUNT          935 a          854 c          880 g          856 t
ORIGIN      
        1 actatgccca gtgcgttgaa tccctgtgtg ccggctgcgg tatgtgtgtc ggtcagtgtc
       61 cctactccgc tctgtcgatg atcatcaacg acgaaggcag aacggtcatg agcgtcactg
      121 ctgccaagtg taaaggctgc ggtacgtgcg gcggtttctg ccccggcggt gctatccgca
      181 tgcagcactt cacgagcccg cagattcttg cccagattga tgcattcttc ctgggaggtg
      241 aacagtaatg gctgatgagt ggaaaccgaa gattatcggt atcatctgca actggtgttc
      301 ctacgccgga gcagacggtg cgggttccgc ccgtacccag tacccgccgg acgtcagaat
      361 cgtccgtgtg atgtgtaccg gacgtatcga cacgctcttt gtgctgaagg cattcgccga
      421 cggagcagac ggagtgcttg tatccggctg tcacttcggt gactgtcact accttgcagg
      481 aaactacaag gcagcaaaga gaatgttcct cgtaaagagc attatgcaga acatgggtct
      541 ggagcacaga agattccgta tgacctttgt ctctgcatcc gaaggagcaa agtgggcagt
      601 ggtgatcaac gatgtgatca acaccgtgaa agagatcgga ccaagcccga ttgcagcaga
      661 aagaaagaga agaggattat aaatatgact acgggaaatc cctccccttt agtctccctt
      721 ttccagaagg aagactttga tgactggtct aaaccccatc tcgtagggga taccactgag
      781 tattctgcac ttctcgaact gcagaagatg cagaaaggcg atcagagaaa atatggtaac
      841 ggtatcattg tcagtgttgt cacctgtact gaaatcaagc agggtattgc aattgaaacc
      901 ggtaaaacat ctccacagta cttcaatgca tgttcaatca ttgaactcca tgaggatgat
      961 ttcaaggctc ttggcattaa caaaaacact aatgtcagag taaccagtgc agctggcagt
     1021 gttatcttaa aggcagttgt tactactcag agtctgtatc ccggacttgc ccacattcca
     1081 atgggaccat gggcaaacat ggttgttccg gcatacacat actccaccgg tgaaccatgc
     1141 ttctgtggtt tcgattgcct catcgaaccg gcacccgaag agaaaataga aaatgcagta
     1201 aaacttctgc acgaaaagtg cggtttgaaa tatgtaggtg acccacacta tgactaaaac
     1261 aattaaaaac gtaatctgta cattctgcgg tactctttgc gatgatcttg aagtcgacgt
     1321 cactgacgac gaaaagaaga ttgtaacggt cagaaatgct tgtgcaattg gtgcagagaa
     1381 atatctccac gtcagcaatc caggacgtgt tgttcttcca cgtatgcgtc aggctgacgg
     1441 tacctataag gatgtttcct acgacgaagc aattgatttc gccgcaaaga cactcctgaa
     1501 agcaaagaaa ccgttattct atggatggtc atccaccaac tgtgaagcaa ttgcagcagg
     1561 aaatgttctt gcagagaaga ctggcggtgt cattgataac tgtgcaacag tttgtcatgg
     1621 ttcttctctc cttgcaattc aggatacggg tgtcccgtcc tgtacgttag gagaagtcaa
     1681 gaaccgtgcc gatcgtgtta tcttctgggg atgcaacccg gcacacgctc acccgcggca
     1741 catgtctaga tattctatct tcccacgtgg tttcttctct gcaaagggac agaaaggaag
     1801 aaagattatc tgtgttgact gccgtgaaac cgacactgca aaagttgcag acacctttgt
     1861 ccgcatcaag cagagctatg actacgaaat cttcgatgca ctcagaactc tccttcgcgg
     1921 tgaagatatc cctgatgaag ttggcggcgt tccaaaggag aagcttcagg aagtcatcga
     1981 catctgcaaa tcaggacgtt tcgttacgct cttcttcgga atgggtctga ctcacactct
     2041 gggaagaaac cacaacatcg acgcaggtat caacttcatc cgtgacttaa acgactacac
     2101 gaaagctgta ctcattgcaa tgcgtggtca ctacaacgtc acgggtgctg gtcaggttct
     2161 tggatggcag tatggtttcc cattcgcatg tgatctgtcc agacttacta tggcacgcca
     2221 caaccccgga gaaacaacct ctgtcgatct cttagtccgt aaggaagttg atgcatgtgt
     2281 cgtcattgca agtgatctcg ctgcccactt cccgttcgaa gcaacccgta ccatggcaca
     2341 catcccgacg attaccatcg atccgcacat cagtatgtcg actgaacttt cggaccttca
     2401 tatcccggtt gctatcgttg gtgttgaggt tggtggatgt tgctatcgta tggacaacgt
     2461 tccaatcgag actcgtaagt gtatcgatgc acctgaagga gtcctgacag atgaagagct
     2521 cttcaaccgc attagtgcac gtgttgatga gatccttgca gaaaacggat gtaaagatgc
     2581 atccgagtac cttgcaaaga tggaggcctg aagatgtccg agattatcat taagaatggt
     2641 cacgtctttg atgctgttac cggtctgaaa ggcgatgtcg ctgatgtttg tattaaggac
     2701 ggaaagtttg ttgacaaagt cagcaatgct gcaaccgtca ttgatgcaac cggcaagact
     2761 gtcatggccg gtgcacttga tgtgcacacc cacgttgcag gtccaaaggt gaaccttggc
     2821 cgctggtacc gcccagaaga caagttccgc cttggtgagc gctgctcggc aaagatcaac
     2881 ccgaagacca agattgaggg aggtttctca attccgacag tctacaagac cggatatgag
     2941 tatgcccgta cgggattcgg cttcctgatg gaagcagcaa tgccgcctct ctactcccgt
     3001 catgtacacg aggagatccg cgacactccg atcgttgatg aagcagcaat gccggtgttt
     3061 ggtaacaatt ggttcatgtt cgagtatctg aagaacggtg aaatcgacaa tgcagcagca
     3121 tatgttgcat ggatgcttcg ccagaccaaa ggatacggta tcaagtgtgt gaacccagga
     3181 ggttccgagg cttgggcatg gggtctgaac tgtatgacca tcaatgaccc ggttccgtac
     3241 ttcgaaatta ccccaaaaga gatcattgct ggtcttatta ccaccaatga agagctgcac
     3301 ctgccacact cagtgcacct gcactccaac aacctgggta atcccggaaa ctatacgacc
     3361 acccttgact ctctgaagat tgcagaggac ttcaaaccga acaataatct tggtcgtaag
     3421 acagtcctgc accacacaca cattcagttc cactcttatg gcggaaccgg atggggtgac
     3481 tttgagtcca agtcgaagga gggtatggac tatggtaaaa gtact
//
LOCUS       DALUAJ010000234         2779 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_46148,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000234 DALUAJ010000000
VERSION     DALUAJ010000234.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46148"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="O0X37_04930"
     CDS             <1..634
                     /locus_tag="O0X37_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62298.1"
                     /translation="YMGDVNYRMNTVFKFGFCAWFMLGTSILLMIGKYAENRFAEIPK
                     GRAVAIAAIIFVVLATLIGCCGIQLGYPGGTLDGAAWLESHHPADAAGIAYLAGIASP
                     GDIVVEAADGSYAYNGRVSAMTGLPTIVGWVGHESGWRSGVGDAGTRWSDVRMIYEDP
                     SKTISLMDQYGAKYLFVGDVEQELYTVNLPDEGLTEIFTADGVSIYQRNN"
     gene            648..1379
                     /locus_tag="O0X37_04935"
     CDS             648..1379
                     /locus_tag="O0X37_04935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HJK62299.1"
                     /translation="MDHPVLTVVIPVYNDAAALREAVPASIEILEGLGLSFEMILCED
                     ASTDGSREVAETFAATDNRIKLNHSDVRRGKGGALSDALAQSRGDIFCFYDVDLSTDL
                     ADLHTLIEKIQAGADIAVGSRFLEESTVLRSGDREMTSVGFNTMVHLLLGSNIRDHQC
                     GFKAFHRDRLAKLIPYVQARGWTWDTEVLAIGQRCGYTIEEIPITWTEGEKTNIRTRD
                     IFSMGWAVLGLAWRIRIIGRYPKDV"
     gene            1531..1899
                     /gene="rpl7ae"
                     /locus_tag="O0X37_04940"
     CDS             1531..1899
                     /gene="rpl7ae"
                     /locus_tag="O0X37_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7Ae"
                     /protein_id="HJK62300.1"
                     /translation="MAKIYQMFEVPEELQNKALEALELARDTGKIKKGANEATKAVER
                     GTAALVLIGADVAPEEIVMHIPGLADEKEIPFVFINKQADIGAACGLDVGCTAVAIVK
                     VGKGKEIVEDLAGQIKALRG"
     gene            1905..2114
                     /locus_tag="O0X37_04945"
     CDS             1905..2114
                     /locus_tag="O0X37_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007314303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S28e"
                     /protein_id="HJK62301.1"
                     /translation="MPDDATPAEVIEVIGLTGMHGEAAQIKCRVLDGPNKGRIITRNT
                     FGPIREGDILMLLETEREAKKLSRR"
     gene            2122..2313
                     /locus_tag="O0X37_04950"
     CDS             2122..2313
                     /locus_tag="O0X37_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24e"
                     /protein_id="HJK62302.1"
                     /translation="MVDIYTCSYCGKQLEPGTGKLFVRKDGAVFYFCSSKCQSNYNLG
                     RIPRRVAWTAAGRKARGKE"
     gene            2318..2767
                     /gene="ndk"
                     /locus_tag="O0X37_04955"
     CDS             2318..2767
                     /gene="ndk"
                     /locus_tag="O0X37_04955"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HJK62303.1"
                     /translation="MERTFVMIKPDGVQRGLVGEILSRFEKKGFKIVAAKFGVLPEAI
                     VDKHYEEHLAKPFYPGMKAYITSGPVFRFVLEGDNVIATVRKMNGATNPAEAAPGTVR
                     GDYALSIGKNVIHASDAPESAAREIDIHFTADELVAYEKIDESQLYE"
BASE COUNT          743 a          638 c          760 g          638 t
ORIGIN      
        1 ctatatggga gatgtaaact accggatgaa cacggtgttt aagtttggat tctgtgcatg
       61 gttcatgctg ggaacatcca ttcttctgat gattggaaaa tatgcagaga accggtttgc
      121 agagattcca aaaggtcgtg ctgttgcgat tgctgcaata attttcgtgg tacttgcaac
      181 acttatcgga tgctgcggga tccagttagg ttacccagga ggaacactgg acggtgcagc
      241 atggctggaa agccatcatc cagcagatgc agcgggtatt gcgtatcttg caggtatcgc
      301 atctcccgga gatatcgtcg tagaggcggc agatggatcg tatgcctaca acgggcgtgt
      361 atcagcaatg actgggcttc ccacgattgt tggctgggtg ggacatgaat ctgggtggcg
      421 aagtggtgtc ggtgatgcag gaacacggtg gagcgatgtt cgaatgatct acgaagatcc
      481 atccaagacg atttctttga tggatcagta cggtgcaaaa tatctctttg ttggagatgt
      541 agaacaggag ttgtacacgg tgaacctgcc tgatgaagga ttaacagaga tatttacagc
      601 agacggtgtg agtatttatc aacgcaacaa ctaacgagtt aggaagaatg gatcatccag
      661 tacttactgt agtaattcct gtctataatg atgcagctgc tctacgcgag gcagtacccg
      721 catcaataga gatactggaa gggctcgggc tgtcgtttga gatgatcctc tgtgaggatg
      781 caagtaccga cggaagccgt gaggtcgccg aaacatttgc agcaacagac aaccgtatca
      841 aactcaacca cagtgatgtc cgcagaggaa aaggaggagc actctcagat gcacttgccc
      901 agtcccgcgg tgacatcttt tgtttttatg atgtggacct ctcaacagat cttgcagacc
      961 tgcacaccct catcgagaaa atccaggcgg gtgcagacat tgcagttgga tcacggttcc
     1021 ttgaagaaag taccgtactg cgttcaggcg atcgtgagat gaccagtgtt ggattcaata
     1081 caatggtaca tctgctcctt ggaagcaata tccgtgatca tcagtgcggg tttaaagcat
     1141 ttcaccgtga ccgactagca aaactgattc cgtatgtgca ggcgcgcggt tggacctggg
     1201 acactgaggt tctcgcaatt ggacaaagat gcggttatac cattgaagag ataccaatca
     1261 cctggacaga gggtgaaaag acgaatatca gaaccagaga cattttttcc atgggttggg
     1321 cggtactggg acttgcatgg aggatacgca ttattggcag gtatccgaag gatgtctaac
     1381 ctgcagatcc taaggttctg tccaaacctt tatggcaaac tctgacccat atatagagtt
     1441 acgccagcaa cggtctgtta ccttaccagg agagccgctg atgagcgatg acggatggtc
     1501 agagaatcat cccgagtgaa gtgagtcatt atggcaaaga tttaccagat gtttgaagtc
     1561 cctgaagaac ttcagaacaa agcacttgaa gctcttgagc ttgcacgcga taccggaaag
     1621 attaagaaag gcgcaaacga ggcaaccaaa gccgtcgagc gcggaaccgc agctctcgtc
     1681 ctgatcggtg ctgatgttgc acccgaagag attgtcatgc acattccggg acttgcagac
     1741 gaaaaagaga ttccgttcgt ttttatcaac aaacaggcag acatcggtgc tgcatgcggc
     1801 cttgacgtcg gatgcactgc tgttgcaatc gtaaaagtcg gcaaaggcaa ggaaatcgtt
     1861 gaggaccttg caggccagat caaggcactc agaggataaa taccatgcct gatgatgcaa
     1921 cgccagcaga agtcattgag gtcatcggcc ttacgggtat gcacggtgag gcagcccaga
     1981 tcaagtgccg tgtccttgac ggcccgaaca aggggcggat catcacccgg aacaccttcg
     2041 gtccgatccg tgagggtgac atcctgatgc tccttgagac agaacgtgaa gcaaagaagc
     2101 tctctagacg gtgagtaaaa gatggtcgat atctatacct gcagctactg tggcaaacag
     2161 cttgagccgg gaaccggcaa actgttcgtc agaaaggatg gtgcagtctt ctacttctgc
     2221 tcctccaaat gccagagcaa ctacaacctc ggtcgtatcc cgcgccgtgt tgcctggacg
     2281 gcagccggac gcaaggcacg cggcaaggag taaaatcatg gagcgcacct ttgttatgat
     2341 caagccggac ggtgtccagc gtggtctggt cggtgagatc ctttcccgct ttgagaagaa
     2401 gggattcaag atcgtcgccg caaagtttgg cgtgcttcct gaagcaattg tggacaagca
     2461 ttacgaggag cacctcgcaa agccattcta cccaggtatg aaggcataca tcacctccgg
     2521 ccctgtgttc cgctttgttc ttgaaggaga caacgttatt gcaactgttc gcaagatgaa
     2581 cggtgcaact aacccagcag aggcagcacc tggaactgta cgcggtgact atgcattgtc
     2641 cattgggaaa aatgtaattc acgcgtctga cgctcccgag agtgcagcac gcgagattga
     2701 cattcacttt actgcagacg agcttgtcgc atacgaaaag atcgacgagt cacagctcta
     2761 cgagtaaaca ccggtattc
//
LOCUS       DALUAJ010000235         5089 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_46273,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000235 DALUAJ010000000
VERSION     DALUAJ010000235.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46273"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            153..1490
                     /locus_tag="O0X37_04960"
     CDS             153..1490
                     /locus_tag="O0X37_04960"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HJK62304.1"
                     /translation="MNERGLFSSRMGFILAAAGAAVGLGNIWRFPYLAAEYGGGIFLL
                     VYLILVVTLGFTIMITEIAVGRSTGTSVVTAFAKLNRKYTFIGYLSLLVPLIILPYYS
                     LIGGWVMKYAAVYFMGQTDTAAADGFFSGFTSLTVEPILWTLLFVALACIVVVFGVEK
                     GIERANKILMPTLIVMLIGLTAYCLSLPGGMDGLWYYIYPDISKFSAETVLAAMGQVF
                     YSLTLGFGVMITYGSYLAKKTDMEKSVRTIEIFDTGVAFLAGLLIIPAVFVFSGGSPE
                     ALGAGPGLMFQALPKVFAMMPFGSAVAAIFFVLVAVAALTSCICMLEVPVAVLGDRFG
                     VSRKTASLLVFLFTMALAVPISLGFGMLDWISVAGMSLLDMFDFFSNNILLPIVAILT
                     CIFIGWIVKPKVVIDEVKSSSPFKAERMYVVMVKFIAPICIAAILIYGLLSVL"
     gene            1580..2494
                     /gene="cofD"
                     /locus_tag="O0X37_04965"
     CDS             1580..2494
                     /gene="cofD"
                     /locus_tag="O0X37_04965"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HJK62305.1"
                     /translation="MITVLSGGTGTPKLIRGLRQILRDNEITVVVNTAEDLWMSGLYV
                     SPDIDTVQYLFSGLLNTDSWWGIRGDSFETFHAMEKLGYTEPLPLGDRDRATNITRAE
                     FLRQGITLTKATDKIAKAYGVQATILPMSDQEVTTYVRTEDGNLMHYQEYWVGRRGNV
                     PITGIVRKTAGDAPLTATPEVIAAIEESEGVIIGPSNPVTSIGPILECAGVREALRKK
                     FTIAVSPFIGSRPVSGPAAALMQAWGYEPTSYGTWEVYKDVVSMFIQDKRDTEIDVPG
                     AHRLDTMMTNEKKAESLAWDLLSYFPRK"
     gene            complement(2783..3367)
                     /locus_tag="O0X37_04970"
     CDS             complement(2783..3367)
                     /locus_tag="O0X37_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HJK62306.1"
                     /translation="MKDFADVVAFHGHSCGGLALGYKACELAAEKLGLHFSEDEEIVA
                     VTETDSCTVDAIQVLLGCTAGKGNLFVNNWGKTAFSFYRRDNNTSIRLVAVPDAVPKN
                     ARMVELRPKIMDGSATPAEDEEYHRLLHEHVHEILATPAEKLFIIKDAVQPLPKEAKI
                     YNNVVCSVCGEQVADGLAQQSEGKYICIPCQMRK"
     gene            3487..4638
                     /locus_tag="O0X37_04975"
     CDS             3487..4638
                     /locus_tag="O0X37_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJK62307.1"
                     /translation="MSVCRRSLAVVLGCIFAILLCLCAGCVSTPETPTEDANTITVTD
                     AFGREVTIPDNPQKIAVSGSGSMRYFVYLDVDLDRIVAVDYQDSSLFSRSNELRPYLL
                     ANPEIKNRQELGSAMAVVDNEKLLASGAEVLFMGGASSSQVEVADEITAKTGIPVVMF
                     YTGDYVTKGDEIRKTLRMLGTILHEEERAEEIIAYFASIEEDIKTRVANVSETDKPTV
                     YVCGISYNGAHGADGTDPTYLPFTLLGAENVAAGIGDISQTGYAKVAKEQILAWDPDI
                     IFVDLGTLTAAEGGALVELWNDPAYQKLSAVKNGNVYTVNPHTSMNVNHETSLANAYY
                     VGKVLYPEQFADIDQAEKADEIYEFVVGAPVFDLLNANVNNLSYQKVEA"
     gene            4705..>5089
                     /locus_tag="O0X37_04980"
     CDS             4705..>5089
                     /locus_tag="O0X37_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJK62308.1"
                     /translation="MTTPDNSDNKTITITDTTGREVVIPADPQRIAVSGSGSTRLVAY
                     LGALDRVIAVDSQDGKTTQTSDIRPYGLANPELRELPTLGSTKGQIDPERLLAVSPDL
                     ILMSTSGADLAGEADQLTEKTGIPVV"
BASE COUNT         1213 a         1206 c         1387 g         1283 t
ORIGIN      
        1 gaggatctgt gcatggcctg ttcctgcgga gcatatccgc ttgagatccc caatgagtca
       61 tgctgtgcgt gccgcaggat aactcctgca aaagatgact aagctgcaga tacctgctgc
      121 aaatatttat tttttcaatc ggtgtgatgc aaatgaatga acgtggtttg ttttccagca
      181 ggatgggatt cattcttgca gcggcagggg ccgcggtagg tcttggaaat atctggaggt
      241 tcccctatct tgcagctgag tacggcggcg gcatttttct tctggtttat ctgattctcg
      301 tggtaacgct cggatttacc atcatgataa ccgagattgc agtcggcaga tcaaccggga
      361 caagtgtagt aacggcattt gccaaactga accgaaaata tacgttcatc gggtacctgt
      421 cgctgctggt tccgctgatc attctcccgt actactcgct tatcggcggg tgggtgatga
      481 agtatgctgc ggtgtacttc atggggcaga ccgataccgc agcagcggac ggatttttca
      541 gcgggtttac ttcgctcacg gttgaaccga tcctctggac actgctcttt gtggcgcttg
      601 cgtgcattgt cgtagtgttt ggtgtggaga agggaattga gcgggcaaac aaaattctga
      661 tgccgacact tatcgtgatg cttataggac ttacagccta ctgtctcagc cttccgggag
      721 gtatggacgg cctgtggtac tatatttatc cggacatttc caagttctcc gcagagacag
      781 tgcttgcggc aatgggccag gtgttttact cgctgacact cggattcgga gtgatgatca
      841 cctacgggtc atatcttgca aagaaaacgg atatggaaaa gtcagtgcgg accattgaga
      901 tctttgatac cggcgttgcg tttcttgcgg gtctgttgat cattccggca gtgtttgtgt
      961 tttccggcgg aagccccgag gcgcttggcg cggggccggg cctgatgttt caggcattgc
     1021 ccaaagtgtt tgctatgatg ccgttcggct cggcggttgc ggcaattttc tttgtgctgg
     1081 tggcggtcgc agcactcacc tcctgtatct gtatgcttga ggttccggta gccgtgctcg
     1141 gtgaccggtt cggtgtgtcc cgaaagacag catcgttact ggtgtttttg ttcacgatgg
     1201 cacttgccgt tccgatctcg ctcgggtttg gaatgctgga ctggatctct gttgccggta
     1261 tgtcgctgct ggatatgttc gacttcttca gtaacaatat cctcctgccg attgtggcaa
     1321 ttctgacctg tatcttcatc ggctggattg taaaaccaaa agtggttatt gatgaggtga
     1381 agagttcgtc gccgtttaaa gcggaacgta tgtatgtggt gatggtaaag ttcatcgcac
     1441 caatctgtat tgcggcgatt ctgatctacg gtctgctttc ggttctctga aagcagccgt
     1501 atcatatttt ttgccagtgt accggcaggg tcaaaacacg gcatcttgat tacgatgcag
     1561 cacccttcta cttagtatca tgataacggt actgtccggc ggaaccggca caccgaaact
     1621 tatccgtgga cttcgccaaa ttctgcggga taatgagatc acagtggtgg tcaataccgc
     1681 cgaggatctc tggatgtcag ggctgtatgt ctcgcccgat attgataccg tgcagtatct
     1741 gttttccgga ctgctgaaca cggacagctg gtggggcatc cgcggcgact cgtttgagac
     1801 atttcatgcg atggagaaac tcggatacac tgagcccctg ccgcttgggg atcgcgatcg
     1861 ggcgacgaac attacccgtg cagagtttct gagacagggg ataactctga caaaagcgac
     1921 ggacaaaatt gcaaaggcat acggggtgca ggcaacgatt ctgccgatgt ccgatcagga
     1981 ggttaccaca tacgtaagaa ccgaggacgg aaacctgatg cactaccagg agtactgggt
     2041 gggacgccgc ggcaatgtgc cgattacagg aattgtccga aaaaccgcag gtgatgcccc
     2101 gctcacagca acgccagagg tgattgcagc aattgaggag agcgagggtg tgattatcgg
     2161 accgtcaaat ccggtaacaa gcatcggtcc tattttggag tgtgcaggtg tccgggaagc
     2221 gttgagaaag aagtttacaa ttgcggtaag tccctttatc ggcagcagac cggtcagtgg
     2281 tccggcggct gctttgatgc aggcgtgggg ctatgaaccg acctcctatg gaacgtggga
     2341 ggtgtacaaa gatgtcgtca gtatgtttat tcaggacaaa cgcgatacgg agatcgatgt
     2401 tcccggtgct caccggctgg atacgatgat gacaaacgag aagaaggccg agtccctcgc
     2461 ctgggatctg ctctcctact tcccgcggaa atgagatcac gtgagatctg tgggatgatc
     2521 tgttccagca tctgaaaaag aaaaatccct ggtcccggtt gtagatcaaa tgaaccggga
     2581 aaaccggcac ccttcaaaga ttgcatgttg ctgtgtggcc ggtgaaaagg gaggtttctt
     2641 ttgctttttg tgcgggcgtt gagaattttc atgatgaaga acagccaaag tgacggtgtg
     2701 gctgcggtgg ggtaatctct ctgcatgatg gtctgtgcag ttctgcggag aaagtacagc
     2761 ccaggtaaaa aaattgtaaa agttatttcc gcatctgaca ggggatgcag atgtattttc
     2821 cctcgctctg ctgtgcaaga ccatccgcca cctgctcacc gcagacggaa cagacgacgt
     2881 tgttgtagat ctttgcctcc tttggcagag gctgaacagc atctttgatg atgaacagtt
     2941 tttccgcagg cgttgcaaga atttcgtgca catgttcatg caaaaggcgg tggtactcct
     3001 catcctcggc aggcgttgcc gacccgtcca tgatcttcgg ccggagctca accatgcgtg
     3061 catttttggg aacagcatcc ggaacggcaa caaggcggat ggaggtgttg ttgtcgcggc
     3121 gatagaacga gaacgccgtc ttaccccagt tgtttacgaa cagattccct ttgccggcgg
     3181 tacagccgag cagtacctga attgcatcaa cggtacagga atcggtctcg gtaactgcta
     3241 caatctcctc atcctctgag aagtgtaggc caagtttctc tgcggcaagt tcgcaggctt
     3301 tgtacccgag tgcaagccct ccgcaggaat gcccatgaaa tgcgacaaca tcagcgaaat
     3361 ctttcatagc aacattatcg ctgtttttcc ttattaatag tattgatatg aaaatatgtt
     3421 cgtcatatca accaacagaa ttccatacat ttattattca gtatgaaaaa atcatagaat
     3481 gataacatga gtgtgtgccg acgatcactt gctgtagtat tgggatgtat ttttgccata
     3541 ctcctctgcc tctgtgcggg ctgtgtttca acgccggaaa cgccaaccga ggatgccaat
     3601 accattaccg tcaccgatgc atttgggcgt gaggttacca tccccgataa tccacagaag
     3661 attgccgtgt ccggatcggg atcaatgcgg tatttcgtgt atctggatgt tgatctcgac
     3721 cgcatcgttg cagttgatta ccaggacagc agtctcttct cccgatcaaa tgaactgaga
     3781 ccctacctcc tcgcgaaccc tgagatcaaa aaccgtcagg aacttggttc tgccatggcg
     3841 gtcgttgaca atgagaaact tctcgcatcg ggagctgagg ttctcttcat gggaggggca
     3901 agcagttcgc aggttgaggt tgccgatgag atcacggcaa agaccgggat tccggtagtg
     3961 atgttctaca ccggtgacta tgttaccaag ggcgatgaaa ttcgcaaaac cctgagaatg
     4021 ctgggaacga ttctgcatga ggaggaacgt gctgaagaga tcattgcata ctttgcatcg
     4081 atcgaagagg atatcaaaac ccgtgtggca aatgtttcgg agaccgacaa accgacggtg
     4141 tatgtctgcg gcatctccta caatggtgcc cacggcgcag acggaactga tcccacttac
     4201 ctgccgttca cactgcttgg tgcggaaaac gtcgctgccg gtatcggaga tatcagccag
     4261 accggttatg ccaaggttgc caaggagcag atcctggcct gggatccgga cataatcttc
     4321 gtggacctcg ggacgctgac cgctgcggaa ggaggagcac ttgtcgaact ctggaacgac
     4381 ccggcttatc agaaactgtc ggctgtcaaa aacggtaatg tgtacacagt aaatccgcac
     4441 acctcgatga acgtgaacca tgagacaagc cttgccaacg cctactatgt tggaaaggtt
     4501 ctctacccgg aacagtttgc cgacatcgat caggcagaaa aagcggatga gatctatgag
     4561 tttgtggtag gagcacccgt gtttgatctg ttaaacgcaa atgtgaacaa cctttcttac
     4621 cagaaagtgg aggcatagat gaaatacctc tcttttcttt tactactgct aacagtagtt
     4681 gtactttctg tttcggcagg atgtgtgaca acacccgata acagcgataa taaaaccatc
     4741 acgataacgg acacaaccgg tcgtgaggtt gtcatccctg ccgatccgca gagaattgca
     4801 gtctccggat ccggttcaac ccggctcgtt gcataccttg gagcactcga tcgtgttatc
     4861 gcggttgata gtcaagatgg aaaaactaca cagacatctg acatacggcc atacggactt
     4921 gcaaatccag aacttcgaga actgcccaca ctcggaagta caaaaggcca gatcgatcct
     4981 gaacggctac ttgcagtatc accggatctg atcctcatga gtaccagtgg agcggaccta
     5041 gcgggagaag cagatcaact tactgagaaa accgggattc ctgtggtac
//
LOCUS       DALUAJ010000236         3430 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_46817,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000236 DALUAJ010000000
VERSION     DALUAJ010000236.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46817"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            149..1219
                     /locus_tag="O0X37_04985"
     CDS             149..1219
                     /locus_tag="O0X37_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HJK62309.1"
                     /translation="MNPENGSAPEHYRAYVGRKIALILGGGILAVIMFFVSVSVGSVS
                     IPIPDIIATLLGWQGTGLYNTIIWNIRIPQALTAVVAGAGLAIAGVAMQSVLRNPLAS
                     PFTLGLSNAAAFGAAIGILMFGAGTTGSSIADAVVVNNPYLTTICAFLFSMLTTVIIL
                     LVAKMRSATPETMVLAGVAISSLFSAGLMAIQYFVDDTKLASIVFWQFGDVSRSSWME
                     LGIITVVVAAAFAYILFKRWDYNAIDAGDETARGLGVNVEKTRLFGMIAASIISAVVV
                     AFLGVIGFVGLVCPHMVRRVIGDDHRFLIPGSFVCGAVLLLISDTVARTIIAPHVLPV
                     AVLTAFLGAPVFLYLIIRGRHM"
     gene            1216..2106
                     /locus_tag="O0X37_04990"
     CDS             1216..2106
                     /locus_tag="O0X37_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK62310.1"
                     /translation="MKDEYPEHKEHHGHHIHEHSHIEGYTHTHEHSHDVPHEHHGETP
                     GKAILNVEGVSFEYRSDPVLQNITVGVARGEILAILGPNGVGKSTLLKCMNLILKPKM
                     GTILLEEMDLTKMSGSEIARNVGYVAQRNESARMTVFDTVLLGRKPHLGWRVTEKDYQ
                     IVDAALKRLGLADMQLRYIDELSGGELQRVCICRAIVQEPSVLLLDEPTSALDLCRQM
                     AILRVIRDVVDHHNVATIMTMHDLNLALRFADRFLFMKDGKVYAACDRSGVTEDMIEA
                     VYGIAVDIVMHKDMPMVIPR"
     gene            complement(2124..3224)
                     /locus_tag="O0X37_04995"
     CDS             complement(2124..3224)
                     /locus_tag="O0X37_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HJK62311.1"
                     /translation="MTRAEIRSLPSSALLARLGSAHLIDAHDQVKAFRRRINVPEDRV
                     EVEGEVHYISARVLPMVLSADREYLLTMATDISDEVADRNRQLLTRELAFILEGISST
                     QEIWEPAMEMLPKISGFEAVAIYQRSIFDDYILFMSKGGGFAPAIPCDSIVDRIIRKG
                     EPAIFDKYRMGLFLEGTLSTMDHAASLILMPIVHESRTVACIVLGSTTPQSPDTVLRG
                     IVMSTAFQISTVASRCLLQEKLQRERDRTRSYLDVAGVMLVVVRRDGIIEMINRYGAK
                     LLGYTENDLIGRNWFDTIVPAEFREERLQSFELMVSGMMNVEDRVYHGPVLCSDGTEK
                     SIRWRNSLLREECGSVSGVVSSGEIISEEEKE"
     gene            complement(3221..>3430)
                     /locus_tag="O0X37_05000"
     CDS             complement(3221..>3430)
                     /locus_tag="O0X37_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62312.1"
                     /translation="VLDEPSNMFLGYNGALRNVTDRRNAEQGVKRWKLFLDGVMDNIP
                     GIIIVTDMKQMLPFTPILQQNSFST"
BASE COUNT          851 a          888 c          886 g          805 t
ORIGIN      
        1 gaacagtttg ccgacatcga tccggcagaa aaagcggatg agatctacga atttgtggta
       61 ggtgtacctg tgtatgatca gctgaacgca aatgtgaaca acctgtcgtt cacaaaactg
      121 gagatccctc tcctgtgatg gaacaaacat gaatccggaa aatggatctg ccccggagca
      181 ttatcgtgcc tatgtgggaa gaaaaattgc actgattctc ggaggaggta tccttgcagt
      241 catcatgttc tttgtatccg tgtcggtcgg atcggtatcc attccaatcc ctgacattat
      301 cgcaacactg ttggggtggc agggaactgg actgtataat accatcatct ggaacatcag
      361 aatcccacag gcactgacag cggttgttgc gggtgcaggt cttgcgattg caggtgttgc
      421 catgcagtct gttctccgaa acccgcttgc ctcaccattc acactcggcc tctccaacgc
      481 cgcagcattt ggtgctgcta tcggcattct gatgtttggc gcaggaacaa ccggcagcag
      541 catcgcagat gcggttgtcg tcaacaatcc gtacctgaca accatctgtg catttctgtt
      601 cagcatgctg acaacggtca tcattctcct ggttgcaaag atgcgttcgg caacacctga
      661 aacgatggta ctcgcaggag ttgcaatcag ttctctcttc agcgcaggat tgatggctat
      721 ccagtacttc gtggatgata caaaacttgc gtccattgtg ttctggcagt ttggcgacgt
      781 atcacgatcc agctggatgg aactgggcat catcaccgtc gtggttgccg cagcattcgc
      841 ctacattctg ttcaaacgct gggactacaa tgcaattgat gcaggagacg agaccgcacg
      901 cggactcgga gtaaacgtag agaaaacaag actcttcggc atgatagcag cttctataat
      961 cagtgcggtg gttgtggcat tccttggggt catcggattt gtgggactgg tctgtcccca
     1021 tatggtgcgg cgggtaatcg gtgatgacca ccggttcctg attccgggaa gttttgtctg
     1081 cggggcagtg cttcttctca tctcagacac cgtggcacgt accataatcg caccgcatgt
     1141 cctgccggta gcagttctga cggcgtttct tggcgcaccg gtgtttctgt atctcatcat
     1201 cagggggcgg cacatgtgaa agacgaatac ccggaacaca aagaacatca tggccaccac
     1261 atccacgaac actcacacat cgaaggatat acgcacacgc acgaacattc tcatgatgtc
     1321 ccgcatgaac atcacggtga gacccccgga aaagccatac tcaatgtaga gggagtgagt
     1381 tttgagtacc ggtccgatcc ggtactgcag aacataaccg tgggtgttgc ccgcggagag
     1441 atcctcgcaa tcctcggacc gaacggtgtc ggcaagtcca cactgctcaa gtgcatgaac
     1501 ctgatcctga aaccaaagat gggaaccatt cttctggagg agatggatct cacaaagatg
     1561 tcaggatcag agattgcacg aaacgtggga tacgttgccc agcgcaatga atcagcacgg
     1621 atgacagtgt ttgacacggt tcttctcgga agaaagccgc atctgggatg gagagttaca
     1681 gaaaaggact accagatcgt ggatgccgca ctcaaacgtc ttggactggc tgatatgcag
     1741 ctccgctaca ttgatgaact atctggcggt gagcttcagc gagtatgcat ctgccgggca
     1801 attgtccagg aaccatcggt tcttctgctg gatgaaccca catcagcact cgatctttgc
     1861 cggcagatgg caattcttcg cgtcatccgc gacgtggtgg atcatcacaa tgtggcaacg
     1921 atcatgacga tgcatgatct caatcttgcc ctgcggtttg ccgaccggtt cctgttcatg
     1981 aaagacggaa aagtctatgc agcatgtgac aggagcggtg tgacagagga tatgatcgag
     2041 gcagtgtacg gtattgctgt tgacattgtg atgcacaaag atatgccgat ggttattccg
     2101 cgttaattcc ataccaccaa aaattattct ttttcctctt ccgaaatgat ttcgccggaa
     2161 gagacaaccc cagagacact cccgcactcc tcacggagaa gcgagttacg ccacctgatc
     2221 gatttttccg taccgtcact gcacagtacc gggccatgat acacccgatc ctcgacattc
     2281 atcatcccgg agaccatcag ttcaaaggac tgaagacgct cttcccggaa ttcagcaggg
     2341 acaattgtat caaaccagtt tctgccgatt agatcgtttt cggtgtatcc gagaagtttt
     2401 gcaccatacc ggttgatcat ctcgataatg ccgtcgcggc gaacgacaac cagcataact
     2461 cccgcgacat ccagataact tcgggtacga tcacgttccc gctggagttt ttcctggaga
     2521 agacagcgcg aagcaaccgt actaatctgg aatgccgttg acatgacgat cccgcgaagt
     2581 accgtgtccg gcgactgcgg cgtggttgag ccgagaacaa tacaggcaac ggttctgctc
     2641 tcatgcacaa tcggcatgag gatgagggag gcagcatggt ccatggtgga gagggttcct
     2701 tcaaggaaca gccccatccg gtacttatcg aagattgccg gttcaccttt ccggattatg
     2761 cgatccacaa tcgagtcaca gggaatggcc ggggcaaatc cacccccttt ggacatgaag
     2821 agaatataat cgtcaaagat acttcgctgg tatatggcaa ctgcttcaaa tccgctgatc
     2881 tttggcagca tctccattgc cggctcccag atctcctgcg tactggaaat cccttccaga
     2941 ataaatgcaa gctcccgcgt caacagctga cggttcctgt ccgcaacctc atcagagatg
     3001 tcagttgcca tggtcaggag atattcacga tccgcggaaa ggaccatcgg gagaacacgt
     3061 gcagagatgt aatgaacctc cccctctacc tcgacacgat cctccgggac gttgatccgt
     3121 ctgcgaaaag cctttacctg atcatgtgca tcgatcaggt gggcggaacc aagacgagca
     3181 agaagtgcgg aggagggaag actccggatc tcggcgcggg tcatgtggag aaactgttct
     3241 gctgcaagat tggagtaaac ggtaacatct gtttcatatc ggttacgatg atgatgcccg
     3301 gaatgttatc cataacacca tcgagaaaca gtttccaccg tttgacaccc tgctcggcgt
     3361 tccgacgatc cgtgacgttt ctcaaagctc cgttgtagcc gaggaacatg tttgacggtt
     3421 cgtcaagaac
//
LOCUS       DALUAJ010000237         2842 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_46889,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000237 DALUAJ010000000
VERSION     DALUAJ010000237.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46889"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            247..723
                     /locus_tag="O0X37_05005"
     CDS             247..723
                     /locus_tag="O0X37_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62313.1"
                     /translation="MGILGQLERALGVWYPDPDADPDDTNPVVSVAFREYVMNRLGSA
                     YSVADREERAVGEPDVVCVRNADDMRFDIITCFRSRMFVGEDGEAYLPWVTPETYTAY
                     LSYAETEKNPCYVIFGLHGFADEPKFLFTVPLAEAVPNLPRTVLQKYEIKKDAALL"
     gene            727..1821
                     /locus_tag="O0X37_05010"
     CDS             727..1821
                     /locus_tag="O0X37_05010"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HJK62314.1"
                     /translation="MKLQIIFRISLFLGAFATMALSNAVVPVLNSIASDPAIQGAVYS
                     AYFLGAFLMVFPAGWMSDRVGRTPLVRAGLCGTLIAAAVLWLSYPDPTAAVLLRFAEG
                     LFTGMFVSAAMAFVNSAADHRKLAGGFVALMNIGMVAGLVVSGWLAAWQAYAGVLVFG
                     ILTGVSGLLSLGPADETPALQTVLPVAEVTEIAVYHKWLWAALFVFCGTTGVVISAYP
                     ELSGSSAEMNGVITALMSVATAVFVYAASRMRTADSLSVVRGAGIFLGVSVPIVLVHP
                     AGMIFVGAAFGVITAAILNYLAETKKPQGVMNGMFNMTQYAGMAALPFAAGLLVLPIG
                     YVAVFLIVAVLNAAVGLLVVRCPCYVHHIQ"
     gene            complement(1856..>2842)
                     /locus_tag="O0X37_05015"
     CDS             complement(1856..>2842)
                     /locus_tag="O0X37_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62315.1"
                     /translation="LMTSTVLGLCVLLFLAVLVIPVSAGVSQSTIAQGDTLYVSGQAP
                     GSQQVALYFFGPNYFKYDTVQVNDGSYTYQLTTTSDMSPSQYYCVVQSPGTGSRFSVS
                     PLTIGQTTYITVNPGSGVPADGSSFVVKGPNALQGSQAAYALEQMIQSPNIPDLSQTF
                     TFTIAYPTITINPIGTQYMGVPFTVSGTTNLAAGDTLLVSVQIATFWPMGKEEANAED
                     AESWSQGDSGSVTVQQGASSGQNYWSFSVNPLHSSQYDVTVTGIKTGATASQEFSVSN
                     HSKPTTVPTTAVTTAVTTAATTVPAPTQSPGFGLAAAAGALGAVVLLAYRRH"
BASE COUNT          648 a          680 c          798 g          716 t
ORIGIN      
        1 acaattgttg cgagaatgaa tcccacaata accgggccga tgatatagaa gaacagtacc
       61 agccaccgtg gaaactgcgc aatgctgaag aaagcatttt tcgtgaaatt ataggcattc
      121 gtgatgatct ctgcgtgttc catgctcgct gatctctctt agaatactat tatgcagttt
      181 tcttataaga taattggcac gctgacgtac tgctttttta agaggacaga ggttcaagaa
      241 gtaagtatgg gcattttagg ccagcttgaa cgggcacttg gtgtctggta ccctgatccc
      301 gatgcggatc cggacgacac aaatcctgtc gtgtccgtag cattccgcga atatgttatg
      361 aaccggctgg gttcggcata ctctgttgcc gatcgtgaag agcgtgccgt aggtgagcct
      421 gatgttgtct gtgtacgcaa tgcagatgat atgcggtttg atatcatcac ctgttttcga
      481 agcagaatgt tcgtcggcga ggatggagaa gcctatctgc cgtgggttac tccggagacc
      541 tataccgcat acctgtccta cgcagaaacc gaaaaaaatc cctgttatgt catattcggt
      601 ctgcacgggt ttgcagacga accaaaattt ctgttcaccg tcccgcttgc agaagcggtc
      661 cccaatctcc ccagaacggt tttgcagaag tatgagatca aaaaagatgc agcccttctc
      721 tgattcatga aactgcagat catcttccgc atatcactgt ttctgggtgc gtttgcaacg
      781 atggcactct ccaacgcggt agtgccggtt ttaaacagca ttgcatctga tcctgcaatc
      841 caaggtgcgg tgtactcagc atacttcctc ggtgcatttc tcatggtgtt tcccgcagga
      901 tggatgtcgg acagagttgg aaggacaccg ctggtacggg caggtctctg tggaacactg
      961 atagccgcgg ccgtcctgtg gctctcctat ccggatccga cggcggcagt tttactccgg
     1021 tttgcggaag gcctgtttac cggtatgttt gtttctgctg caatggcgtt cgtgaactca
     1081 gcagcggatc accggaaact cgccggcggg tttgttgcac tgatgaacat tgggatggtt
     1141 gcaggactcg tcgtgagcgg ctggcttgca gcgtggcagg cctatgcagg tgttctggtg
     1201 tttggcatac tgacgggtgt ttctggactg ctgagcttgg gacctgctga cgagacccca
     1261 gcacttcaga cggttctgcc ggttgcagag gtaacggaga ttgccgtgta tcacaagtgg
     1321 ctgtgggcag cactctttgt gttctgcgga acaaccggcg tggtcatctc cgcctatccg
     1381 gaactctccg gatcctcagc cgagatgaac ggagttatta cagcactgat gagtgttgca
     1441 acggcggtgt ttgtgtatgc cgcatcccga atgcgaaccg cagactccct ttccgtagtg
     1501 agaggcgcag gcatttttct tggggtgtcc gttccgatag tattggtaca cccggccgga
     1561 atgatctttg tgggagcagc atttggtgtg attactgctg caattttaaa ctatcttgcg
     1621 gagacaaaaa aaccgcaggg tgtgatgaac gggatgttca acatgacaca gtatgcgggg
     1681 atggctgcac tgccgttcgc cgccgggctg ctggtcctgc cgatcgggta cgttgcggtg
     1741 tttctgattg ttgcagtact gaatgcagcg gtcgggctgc tggtcgtccg ctgtccctgt
     1801 tatgtacacc acatacaatg aaagacccgt ttgggaacga ccctgaaaaa aggagttagt
     1861 gtcttctgta tgccaagaga acaacagcac caagtgcccc tgctgcggca gcaagaccaa
     1921 atcccggtga ctgggttggg gccgggacag tggtcgcagc cgtggttaca gcagtcgtta
     1981 ccgcggttgt tggaacagtg gttggtttac tatggttaga aaccgaaaac tcctgcgatg
     2041 cggtcgcacc ggttttaatg ccggttaccg tgacatcata ctgtgatgag tgcagcgggt
     2101 tgacggagaa gctccagtag ttctggccgc ttgaggctcc ctgctgaacg gttacacttc
     2161 cggaatctcc ctgggaccag gattcagcat cctctgcatt agcttcctct tttcccatag
     2221 gccagaacgt ggcaatctga acgctgacaa gaagtgtatc acctgctgca aggttggtgg
     2281 ttccggaaac cgtgaaaggt actcccatgt actgagttcc tatcgggttg atggtaatcg
     2341 tcggataggc aatcgtaaag gtgaatgtct gggaaagatc cggaatgttc ggactttgga
     2401 tcatctgctc cagagcatat gctgcctgtg aaccctgaag tgcgttgggg cccttcacaa
     2461 caaaactgct gccgtcggcg ggaaccccgc ttcccgggtt gaccgtaata taagtggttt
     2521 gaccaatggt cagaggacta acactaaacc ggcttccggt acctggactc tgaacaacac
     2581 agtaatactg ggaaggggac atatcgctcg tggttgtgag ctgataggta tatgatccgt
     2641 catttacctg aacagtatca tatttgaagt agttaggacc gaagaagtac aaggcaacct
     2701 gctgggaacc gggtgcctga ccgcttacat aaagcgtgtc tccctgagca attgtactct
     2761 gggaaactcc cgcagagacc ggaatcacga gtactgcaag aaacagaagg acacagagac
     2821 caagaacagt actggtcatg ag
//
LOCUS       DALUAJ010000238         3315 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_47105,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000238 DALUAJ010000000
VERSION     DALUAJ010000238.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47105"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(89..1066)
                     /locus_tag="O0X37_05020"
     CDS             complement(89..1066)
                     /locus_tag="O0X37_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosine biosynthesis radical SAM protein
                     RaSEA"
                     /protein_id="HJK62316.1"
                     /translation="MSAPIKPLASWTGQDRYFGEVLPSLTGIFLSGGCSWNRCRMCGY
                     KNDRNTCGSRQDLIDHMTAQIAWLAENYASDTYGLAKIFTSGSVFDPVEIPREVLDGF
                     GSLFAGKPVIAESRAEYVTEDALSRFLSTLDQGQSHPLTVAMGLETTNDAIREKSIDK
                     GFSFADFIRASEICHDAGVGVKAYLMMKPLFLTEREAMEDMQKSIAEAAPYADMISMN
                     LCTVQGRTELEQYWQRGSFRPPYLWSALKVLLDAEVSVACDPVGGGFRRGPHNCGICD
                     KEIVAAINEYSLTADKSVLQAVWDAGCSCRKEWEFVLDNETSWNMPLTE"
     gene            1228..2376
                     /locus_tag="O0X37_05025"
     CDS             1228..2376
                     /locus_tag="O0X37_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62317.1"
                     /translation="MNNGTRKTRILLLAAGLLVLLCCAGTVSAYSFDFVTPDSQTATL
                     KMGVGSPIVVECVTDNIPGGATIRIVLAKSGGSGSVVESKSVVIQSDGRFGVTFDTWD
                     LDGGMYTVTVQEPKDYPFGSKRNWFSVQLVDRTGELTSTSPRVQYSTDEVLVAGKASE
                     TGSRGVELTMYEGTTTDGRVVYGPTWVGTNENGQFSETLPATGAGVYQVVVKDKDGYI
                     GLLTYTLISGSATSTSTTTATPTSSGMTLTANAEASYDSPAYFAVTTGTGTATITAFR
                     GVDWTVEYTVNNGAVVKINEKDENADETITITTSDATVYLKVYPTNEGASGTVYLTAK
                     NVLSFAEEPGLADSFGTGAGPNATQSPVGAVVAVIGVLAAAALILSRR"
     gene            2429..2512
                     /locus_tag="O0X37_05030"
     tRNA            2429..2512
                     /locus_tag="O0X37_05030"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2463..2465,aa:Ser,seq:tga)
     gene            2564..2806
                     /gene="hypC"
                     /locus_tag="O0X37_05035"
     CDS             2564..2806
                     /gene="hypC"
                     /locus_tag="O0X37_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013329239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HJK62318.1"
                     /translation="MCIAVPAEILEIRDGNVALVDYGDLQQEVRIDLVDVKVGEFVLV
                     HVGFAIQRLSREEGLETRELFKEVYKAMEEGPESHA"
     gene            2799..3137
                     /locus_tag="O0X37_05040"
     CDS             2799..3137
                     /locus_tag="O0X37_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HJK62319.1"
                     /translation="MHESGIAYDIYATSKRAAEENGAVRVKTIYVDVGSMAMVNPDQV
                     EFMFGTFVADDLLFSEAKLVFTTVPPVAECECGYRGSEIFVCPTCGKLPHLVQGREIL
                     VKNIEIDTEE"
     gene            3139..>3315
                     /locus_tag="O0X37_05045"
     CDS             3139..>3315
                     /locus_tag="O0X37_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HJK62320.1"
                     /translation="MKKDTEVNMMHGAGGEVMGELLVTLTAFKHNNAGGIGLESLDDG
                     STFEIGGKTVVLTTD"
BASE COUNT          855 a          837 c          894 g          729 t
ORIGIN      
        1 cgttcatgcc gctgctgctt gcagaactgc gggaactttc cgcataatct tttttcaaaa
       61 cagaagttac agaaaaacgg cgaaaaaatt actcggttaa gggcatgttc cacgacgttt
      121 cattgtcaag aacaaactcc cactccttcc tgcaggaaca gcccgcatcc cagaccgcct
      181 gaagaacgct cttgtcagca gtgagcgagt actcattgat cgcagcaacg atctctttgt
      241 cacagatacc gcagttgtgc ggaccccggc ggaacccgcc gccaaccgga tcacaggcaa
      301 cactgacctc ggcatccagc agaaccttca gggcagacca cagatacggc gggcggaacg
      361 aaccccgctg ccagtactgc tcaagctcgg ttctcccctg caccgtacac agattcatcg
      421 agatcatatc cgcatagggg gcagcttccg caattgactt ctgcatatcc tccattgcct
      481 cccgttccgt caaaaacagc ggcttcatca tcagatacgc cttcacgccg acgcccgcat
      541 catgacagat ctcggatgca cgaatgaaat ccgcaaagga aaaacccttg tcgatggact
      601 tctcacggat agcatcattc gtcgtttcaa gacccattgc aacggtcagc ggatgagact
      661 gcccctgatc cagagtggac aaaaaccgtg aaagggcatc ctcggtcacg tactcggcgc
      721 gcgactcggc aatcaccggt tttccggcaa agagactgcc aaatccgtcc agaacctcgc
      781 ggggaatctc gactggatca aatacactgc ctgaggtgaa tattttggca agcccgtacg
      841 tatcggaggc gtagttctcc gcaagccagg caatctgggc agtcatatga tcgattaggt
      901 cctggcgcga accgcaggtg ttgcgatcat ttttgtaacc gcacatcctg cagcggttcc
      961 agctgcatcc cccggaaaga aaaatgccgg ttaatgacgg cagaacctcg ccaaaatatc
     1021 tgtcctgtcc cgtccaggag gcaagaggtt ttattggtgc tgacatgact cttatctcat
     1081 ggtattgcag gcattatctt agtttcccag aacacaggat ggagtccaga aaaatccggg
     1141 aggagatgca gaaaagacct ggtttcagga taaaacagag aacgcggaca acaaagccta
     1201 tctatataga aaagaactat ttagattatg aataacggta cgagaaaaac aaggattctc
     1261 cttctcgcgg ccggtctgct ggttctgctg tgttgtgccg gaactgtatc ggcatactca
     1321 tttgattttg tcacgccgga cagccagacc gccacgctca aaatgggagt gggatctcca
     1381 attgttgttg aatgtgtaac cgacaatatc cctggaggtg ccaccatacg tattgttctg
     1441 gcaaaatcag gcggaagtgg aagtgtcgtt gaatcaaagt cagttgttat tcagtcggac
     1501 ggccgattcg gagtaacctt tgatacctgg gatctggacg gaggtatgta tacggtaacc
     1561 gttcaggaac cgaaagatta cccgtttgga agtaaacgta actggttcag cgtgcagctg
     1621 gttgacagaa caggtgaact gaccagcacc tcacccagag ttcagtactc caccgacgag
     1681 gtactggtcg caggcaaagc aagtgagacc ggatcacgcg gtgtggagct gacgatgtat
     1741 gagggaacca ccaccgacgg tcgtgtagta tacggcccta cctgggttgg gacaaacgag
     1801 aacggccagt tctctgagac actcccggca acaggagccg gtgtttatca ggttgttgtc
     1861 aaagacaaag acggatacat tggattactg acctacacgc tgataagcgg aagtgcaacc
     1921 tccacctcga ccacaactgc aaccccgacc tcctcaggaa tgacgctgac cgcaaatgca
     1981 gaggcatcct atgacagccc ggcatacttt gcggtaacga ccggaacagg gaccgcaacc
     2041 atcaccgcat tccgcggcgt tgactggacg gtcgagtata ccgttaacaa cggtgcagtt
     2101 gtcaagataa acgagaagga tgaaaacgcc gatgagacga tcaccatcac aacaagcgat
     2161 gcaaccgtct atctgaaggt gtatccgacc aatgaaggtg caagcggaac ggtctacctc
     2221 accgcaaaga atgtactctc ctttgccgag gagcctggcc ttgctgacag ctttggaaca
     2281 ggggccggac cgaatgcaac ccagagcccg gttggtgccg tagttgcggt gatcggtgtt
     2341 cttgcggcag ctgctcttat cctttcccga agataagaca acacaaatct ttttttatgc
     2401 agcatgcata ttatgtgtgg actttagtgc cgagatagtc tagtccggaa aggcggtggc
     2461 cttgaaagcc tctggtggta acacctcggg agttcaaatc tccctctcgg cgtatatccc
     2521 ttttcgttgt ctttatcact ctttctcacc aatataccgg tatatgtgca tagcagttcc
     2581 cgccgaaata ctggaaatac gcgatggaaa tgtggcgctc gttgattacg gagatcttca
     2641 gcaggaggtc agaattgatc tggttgatgt gaaggtcggc gagtttgtgc tggttcacgt
     2701 gggttttgcc attcagcgtc tgtcccgtga ggagggactg gagacccgcg aactcttcaa
     2761 ggaagtctat aaggcaatgg aagaggggcc ggaaagccat gcatgagtcc ggtatcgcat
     2821 atgatatcta tgcgacctca aaacgtgctg ccgaggagaa cggggcagtg cgggtcaaga
     2881 cgatctacgt ggacgttgga tcgatggcca tggtaaaccc tgatcaggtg gagtttatgt
     2941 tcggaacgtt tgttgcggat gacctgctgt tttccgaggc gaaactggtg tttaccacgg
     3001 tgccgccggt tgccgagtgc gagtgcggat accgcggctc ggagattttt gtgtgtccca
     3061 catgcggaaa gctgccgcat ctggtacagg gacgcgagat tttagtgaag aatattgaga
     3121 tagatacgga ggagtaagat gaaaaaggat acagaagtca acatgatgca cggcgcaggc
     3181 ggcgaggtga tgggagaact gctggtgacg ctgacggcat tcaaacacaa caatgcagga
     3241 ggtatcggac ttgagtcgct ggacgacggc tcaacgtttg agatcggcgg caagaccgtt
     3301 gttctgacaa ccgac
//
LOCUS       DALUAJ010000239         2084 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_47587,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000239 DALUAJ010000000
VERSION     DALUAJ010000239.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47587"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            214..1275
                     /gene="carA"
                     /locus_tag="O0X37_05050"
     CDS             214..1275
                     /gene="carA"
                     /locus_tag="O0X37_05050"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HJK62321.1"
                     /translation="MKAVLGLEDGTIITGSGFGVEGAATGELVVTLVSNGYMEALSDP
                     SVAGQLLMFGYPLVGNYGANPDQLQSSKVHATGAIVRELCEFPKHEPTMAAYFEENGL
                     MGIEGVDTRMLTIKLREEGVMRAALLTGSDDGHEAVRLAQNAPVQETRELIPDVTCKE
                     AYHIPGSGKKIAVLDLGCRKSILKSLQNRGADLYVYPYGTPADVILADKPQALFLTNG
                     PGYPFAAPKAIETVKNILGTIPVQGVCMGTEIIAAALGGEVEKLKLGHRGASQPVKFS
                     DGSVAVTFQGHGYAVIGDTLPEGCEVSCVNLNDHTVEGFINNDLGVYCVQFHPEHDAV
                     HDGAEKPIYDIMYRGIPDA"
     gene            1268..>2084
                     /locus_tag="O0X37_05055"
     CDS             1268..>2084
                     /locus_tag="O0X37_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HJK62322.1"
                     /translation="MPKNPTLKKVLLIGSGPIQIGQAAEFDYAGSQACKAVREEGIEV
                     VLVNSNPATIQTDPETADKVYVEPLKAEIIAEIIKKEKPDGILSGMGGQTGLNLTAEL
                     YEMGALEGVQILGTPLEAIYHGEDREMFKNLMLEIGEPVPRRFILTKMEQLEEAYETV
                     GLPAIIRPAYTLGGSGGGVAETKEELKKIVEHGLAKSRVHQVLIEESVKGWNEIEFEV
                     MRDAADTCIIICGMENVDPMGVHTGESVVVAPILTLTADQFGIMRRAAIKIIRA"
BASE COUNT          560 a          543 c          521 g          460 t
ORIGIN      
        1 atactatact acttgtgttg gaaagggaaa cacctatcgg aaaaacctga tttgcggagc
       61 tggaaaaata tatttatttg agacttccta tttagataaa ttataaaata tataatattt
      121 cgaatatacg catattttat tcgattaaat atatatgaat catagattac ccttattacc
      181 tcccgacgcg gacatataag atggtactaa atcatgaagg cggtcttagg acttgaagat
      241 ggaactatca tcactggaag cggcttcggt gtcgagggcg ctgcaaccgg tgaactggta
      301 gtaaccctcg tatccaacgg atacatggaa gccctcagtg acccaagcgt tgccggacaa
      361 ttattaatgt tcggttatcc gcttgtggga aactacggcg caaaccccga ccagctccag
      421 agcagcaaag tgcatgcaac cggcgccatt gtccgtgagc tttgtgagtt ccctaagcac
      481 gagccaacga tggctgcgta tttcgaggag aacggcctca tgggaatcga aggagtcgat
      541 acccggatgc tcaccatcaa actcagagag gaaggtgtca tgcgagcggc tcttcttacc
      601 ggctccgatg acggtcatga agccgtccgc cttgcccaga atgctcctgt gcaggagacc
      661 cgcgaactca ttccggacgt cacctgcaaa gaagcctacc acattcccgg atccggcaaa
      721 aagatcgcag ttcttgatct cggctgccgg aaatccattc tgaagagcct gcagaaccgc
      781 ggtgccgacc tctacgtcta cccctacgga acgcctgccg atgttattct tgcagacaaa
      841 ccgcaggcac ttttcctgac caacggtcct gggtatccgt ttgccgcacc gaaagccatt
      901 gaaaccgtca aaaatattct cggtacaatt cctgtacagg gtgtctgcat gggaaccgag
      961 atcattgcag ctgctctcgg cggtgaggtt gagaaactca agcttggtca ccgcggtgca
     1021 agccagccgg taaagttctc cgacggatcg gttgctgtca ccttccaggg gcacggctat
     1081 gctgtaatcg gcgacactct tcccgaaggc tgcgaagtct cctgtgtcaa cttaaatgat
     1141 cacaccgttg agggattcat caacaatgac ctcggcgtgt actgtgtgca gttccacccc
     1201 gaacacgatg ctgttcacga cggagcggaa aaaccaatct acgacatcat gtacagagga
     1261 atccccgatg cctaaaaacc caacactcaa aaaagtcctc ttaattggtt ccggaccgat
     1321 ccagatcgga caagcagctg aattcgacta cgcaggaagt caggcctgta aagctgtccg
     1381 tgaggaggga atcgaggttg ttctcgtcaa ctccaacccg gcaaccatcc agacagatcc
     1441 agaaactgca gacaaagtct atgttgagcc gctgaaagcc gagatcattg ctgaaattat
     1501 taaaaaggaa aaaccggatg gaatcctctc agggatgggc ggccagaccg gtctcaacct
     1561 caccgcagaa ctttatgaga tgggcgctct tgaaggtgtc cagatcctcg gaacacctct
     1621 tgaggcaatc taccacggtg aagaccgtga gatgttcaag aacctcatgc ttgaaatcgg
     1681 cgaaccggtt ccaagacgtt tcatcttaac caagatggag cagcttgagg aagcctacga
     1741 gaccgtcggt cttccggcaa ttatccgtcc cgcatacacc ctcggaggat ccggcggagg
     1801 agttgccgaa acaaaagaag aactcaaaaa gatcgttgag cacggtcttg ccaagtcccg
     1861 tgtccaccag gtcttaatcg aagagtcggt caaaggctgg aacgagattg agttcgaggt
     1921 catgcgggat gcagctgata cctgtatcat catctgcggt atggaaaacg ttgacccgat
     1981 gggagtacac accggtgagt ccgttgtggt tgcaccgatc cttaccctga ccgcagacca
     2041 gttcggcatt atgcgccggg ctgcaatcaa gatcatccgt gcac
//
LOCUS       DALUAJ010000240         1573 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_47704,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000240 DALUAJ010000000
VERSION     DALUAJ010000240.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47704"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="O0X37_05060"
     CDS             <1..819
                     /locus_tag="O0X37_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62323.1"
                     /translation="VPDQPENRGIEKLFEQVSPDILRATAERSRQEAVLSYPWTSPFT
                     GTTSRGSLRIVHRVISSEDFLRIHAAARSLGATVQDTLLAAFASALQTLGSSDPVPLE
                     SVMDLRPALGPGVSGWVGNFSANYRLTLSCRDGLRETVLQSAAFGRSFKANHPEVVCA
                     LRFLDPSVRREMDILLPEDVPGLDLRSPFLSNVGVIPSSVGSFGDEITVSAVWIVTDF
                     VSGRYPSLVSGTWNGSLHLSAAVAFADTSADRIFDQMISSLFSLVDRKEGHISE"
     gene            1069..>1573
                     /locus_tag="O0X37_05065"
     CDS             1069..>1573
                     /locus_tag="O0X37_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62324.1"
                     /translation="MKSSYKLVIFLGVFVVVLLIAVAAVPMGASEEPVNPSMSSAEYL
                     AMYWPEVYVQLSSEDKNVLSAIPHVWEYKEIKKTDGGSGLGMTFADPQAVERYALMDV
                     VSDLNISEAEYMQIVWPELWTDMSTATKTHLATLPNMHSTAMKGIMVGGDFSRSFSLM
                     VTVGTTFE"
BASE COUNT          326 a          371 c          397 g          479 t
ORIGIN      
        1 gtaccggatc agcctgagaa ccgcggtatt gaaaaactgt ttgagcaggt ttctcctgat
       61 attctccggg ccacagcaga acgatcacgg caggaagctg ttttgtctta tccgtggaca
      121 tctccgttta ccggaacaac ttcccgcggt tcgcttcgta ttgttcacag agtgatctct
      181 tccgaggatt ttctccggat acatgcagct gcccgcagcc ttggtgcaac agttcaggat
      241 accctgctcg cagcatttgc gtccgccctt caaacactgg gctcctctga tcccgtccct
      301 cttgagtcgg tgatggatct gcgtcctgct cttggtcccg gagtgtcggg atgggttgga
      361 aatttttcgg caaactaccg gcttacactt tcctgtagag acggcctgcg ggaaactgtc
      421 ttacaatctg cggccttcgg cagatccttc aaggcgaatc atcccgaggt tgtgtgtgcc
      481 ctccggtttt tggatccatc agtccgccgg gagatggaca ttcttctgcc ggaggatgta
      541 cctggtctgg atctccgcag tccgttcctt tccaatgttg gtgtgatccc gtcctcggtt
      601 ggttcgtttg gagatgagat cacagtttct gcggtatgga tcgtgacgga ttttgtctca
      661 ggacgttatc cgtccctcgt ttcggggacc tggaacggca gtctgcatct ttctgcagcc
      721 gttgcttttg ctgatacctc tgcggaccgg atttttgatc agatgatctc ttcacttttt
      781 tctcttgtgg ataggaaaga ggggcacatc tctgaataag ggcactttca gtacgcgtgg
      841 tcgggagtat atatatctcc gaaacgtatt ctgttttgta acaccccaag gagctgcgga
      901 cctctgacag tacccgaggt ttggtccttc atgagtcccc tccgctctct tttctttaac
      961 gtgtttgatt tgccctaacc ttttgacaga aatgagagag ttttcttaat ccagtacttc
     1021 cagtttgttg tatatcagga atattcctgc ttgaactggt gaattattat gaagtcatct
     1081 tataaactag taatcttcct cggagttttc gtcgttgttc tgctgattgc agttgcagca
     1141 gttccaatgg gtgcttcaga ggaaccagtc aatccatcga tgagttctgc ggagtatctt
     1201 gctatgtact ggcccgaagt gtatgtacag ctctcgtccg aggacaagaa tgtactttct
     1261 gctattcctc atgtatggga atacaaagag atcaaaaaaa ccgacggtgg gtctggtctt
     1321 ggcatgacat ttgcggatcc acaggcagtt gaacggtatg ccttgatgga tgtcgtttcg
     1381 gatcttaata tctctgaagc tgagtatatg cagatcgtgt ggcctgaatt atggacagac
     1441 atgagtactg cgacgaagac acaccttgca actcttccaa acatgcattc tacggctatg
     1501 aagggaataa tggttggggg ggattttagt cgctcttttt cattgatggt taccgttgga
     1561 actacttttg aaa
//
LOCUS       DALUAJ010000241         2383 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_47710,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000241 DALUAJ010000000
VERSION     DALUAJ010000241.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47710"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            108..1316
                     /locus_tag="O0X37_05070"
     CDS             108..1316
                     /locus_tag="O0X37_05070"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HJK62325.1"
                     /translation="MTDTPSAGGKGTIVLAFSGGLDTSVCVPILKNMYGYDRVVTVAV
                     DVGQPAAEVKMADDKGKLIADKHYTIDAKDAFVKQHVFPAIKANALYEGYPMGTSLAR
                     PLIAEEIVKIAKQEGATAIGHGCTGKGNDQLRFDFIFRMHGFDIVAPMREHNMTRDWE
                     IDYAEQHGIPIPVKKSTPWSVDENIWSRSIEGGRLEETDYHPPEEIYHWTTSPEAAPD
                     KPEIVSITFENGVPVALNGKKMNGVDLIVELNTIAGRNGVGRNDMVEDRVLGLKAREN
                     YEHPAATVLIAAHADLEKLVLSRFELKFKHIVDDQWAELAYMGLVHEPLYHDLNAFID
                     ETQKRVTGTVDVQLFKGGLHILGRKSPNAIYSQDMVSFDTTTIDQRDAIGFSKYFGLQ
                     GRMVWQLQNK"
     gene            1376..2314
                     /gene="aglJ"
                     /locus_tag="O0X37_05075"
     CDS             1376..2314
                     /gene="aglJ"
                     /locus_tag="O0X37_05075"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer glycoprotein N-glycosyltransferase AglJ"
                     /protein_id="HJK62326.1"
                     /translation="MDFSKDDVCVLIPTLNEKATIGPVIEELQKEGYHHILVVDGHST
                     DGTPEIAKKLGATVMTQEGKGKGAAMIEAFEKITSPYILMLDGDGTNPPEYADAMIEP
                     LVSGRADHVIGNRLNQYERGALTRLNLWGNRIMNTLFKWAHGVYMTDILSGYRAFTLE
                     SVKQMNLKEAGFEIETEISSAVVHQGLRFEVVPTFYKKRPGSPTKLHPIRDGYKILRA
                     INKYGKMNNPLFHFSVIGVLMGIAGFCLGLFVLFEWLAGIEHLFMGILTMLLIVAGIL
                     TFMIGFISDMILGYHREQMRELRMLREKLDEMKNKE"
BASE COUNT          680 a          571 c          594 g          538 t
ORIGIN      
        1 gtatagtaaa agcatacgga tccaaacgta tccagtaact gggacacatt gaatatactc
       61 aacacccaat atgaatcaca catttccaaa aatggagatc tacatcaatg acagatacac
      121 catcagccgg aggaaaaggc acaattgttc tcgccttttc cggaggactt gatacctccg
      181 tctgtgtgcc gatcttaaag aacatgtacg gttacgaccg tgtggttacg gttgccgtag
      241 atgtcggtca gccagcagcc gaagtaaaaa tggcggatga caaaggaaag ctcatcgccg
      301 acaagcatta cacaatcgat gcaaaagatg cgtttgtgaa acagcatgta ttccctgcaa
      361 ttaaggcaaa cgcactgtac gaagggtatc caatgggtac atcccttgca cgtcccctga
      421 tcgcagagga gattgtcaag atcgcaaagc aggaaggagc aacagccatc ggtcacggat
      481 gtaccggtaa aggaaatgat cagctgcgtt ttgatttcat cttccggatg cacggctttg
      541 acattgttgc accgatgcgc gagcacaaca tgacccgtga ctgggaaatc gactacgcag
      601 aacagcacgg cattccgatt ccggtcaaga agtccacacc gtggagtgtt gatgagaaca
      661 tctggtcacg cagtattgaa ggaggcagac tcgaagagac ggactatcac ccgccagagg
      721 agatctacca ctggaccacc tcaccggaag ctgcgcccga caagccggag atcgtatcca
      781 tcacctttga aaacggtgtc ccggttgccc tgaacggcaa aaagatgaat ggtgtcgatt
      841 taatcgttga actgaacacg attgccggca gaaacggagt tggcagaaac gatatggtgg
      901 aagaccgtgt ccttggactc aaggcccgtg agaactacga acacccggca gcaacggttc
      961 tgattgccgc acacgccgat cttgaaaagc tggttctttc ccgctttgag ctcaagttca
     1021 agcacattgt ggatgaccag tgggcagagc tcgcctacat gggtcttgta cacgagccac
     1081 tctaccatga tctgaacgca tttattgacg agacgcagaa acgtgtcacc ggaaccgtgg
     1141 acgttcagct gtttaaggga ggacttcaca ttctcggcag aaaatcaccg aatgccatct
     1201 actcacagga catggtttcg tttgatacaa caaccatcga tcagcgtgat gcaatcggct
     1261 tctccaaata cttcggactc cagggcagaa tggtctggca gctccagaac aaataatctt
     1321 ttttcccgta catctgtaag actgattaca gagaacatcc aaagaatatc atagaatgga
     1381 cttttccaaa gatgacgtct gtgtgttaat cccgacactc aacgaaaaag ccaccattgg
     1441 acctgttatc gaggaactgc aaaaagaggg ataccaccat atactcgtcg tggacggcca
     1501 cagtacggac ggaacgccgg agattgcaaa aaaacttggt gcaaccgtta tgacgcagga
     1561 gggaaaagga aaaggtgcgg ccatgatcga agcatttgaa aagatcacct ctccctatat
     1621 cctgatgctt gacggggacg gtaccaaccc gccggagtac gcagatgcca tgatcgaacc
     1681 gcttgtaagc ggcagggccg atcatgtcat aggtaaccgg cttaaccagt acgagcgtgg
     1741 cgctctgacc cgcctgaacc tttgggggaa ccggatcatg aacacactgt ttaaatgggc
     1801 tcatggtgtg tatatgacag acattctttc cgggtatcga gcattcaccc tggaatcggt
     1861 caagcagatg aatctcaagg aagcaggatt tgagatcgag accgagatca gttccgcagt
     1921 agtccatcag ggtctcaggt tcgaggttgt gccgacattc tacaaaaaac gtccggggtc
     1981 tccaacaaaa ctgcacccaa tccgggacgg atacaaaatt ctccgtgcaa ttaacaaata
     2041 cggtaaaatg aacaatccgc tcttccattt cagcgtcata ggtgttctga tgggtattgc
     2101 aggattctgc ctcgggcttt ttgtgctatt tgagtggctg gccgggattg aacatctgtt
     2161 tatgggtatt ctgaccatgc ttctcattgt tgcaggaatt ctcaccttca tgatcggatt
     2221 catcagtgac atgatcctcg gctatcaccg cgaacagatg cgggagttgc ggatgctccg
     2281 ggaaaaactc gacgaaatga aaaataaaga gtgaactgtt tttcgtgagg aagagaatcc
     2341 tcacattcaa atttttgcgg gttgattatg tacatagtat gac
//
LOCUS       DALUAJ010000242         4123 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_48059,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000242 DALUAJ010000000
VERSION     DALUAJ010000242.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48059"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="O0X37_05080"
     CDS             complement(<1..678)
                     /locus_tag="O0X37_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HJK62327.1"
                     /translation="MTTLQEYLKNSGVYEDLSKIISLIADQAGPIRAAFISNQNYADS
                     KNASGEQQAAMDTWADSHITRVLAKSGLVRAVASEEQDDITTFSSSAKYSVVMDPLDG
                     SSLISVNLCVGTIVGIYDGDVLQPGKNLKAAFYILYGPMTTLTLSVGHGVAIFAQDSD
                     GVYQLLSDNVKMPEGNLYGSGGLRTEWLPEHAKFISDVEGAGGKNRYSGSFVADFHQV
                     LKYGGVYA"
     gene            complement(785..2491)
                     /locus_tag="O0X37_05085"
     CDS             complement(785..2491)
                     /locus_tag="O0X37_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HJK62328.1"
                     /translation="MTETQGSPKIGGNIGNYDETYRTFSLVTPKYYNFAFDVVDAWAK
                     KDRNHLAMIWVNQLGVEKKFTFWDMMIHSNEAANILMKFGIQKGDRVLLMLPRVPEWW
                     ILVLGIMKLGAVFCPSPHMLTVKDIAYRIKVGNFKMVITDSDNMDKVDEVAADCPNLQ
                     LRMLVDDKPGERLPTPWIGYQNELLYPAPVSTKLVSSVGRKMLSTDPMLIYFTSGTTK
                     DPKMVLHDYGHPLGQTVTAKFWHDLTEYDVHFTVSDTGWAKCGWGKIYGQWICGACIF
                     VYDYRTKFHATELLPLIERYGITSFCAPPTIYRMLIIADLKKFSFSELRTCTSAGEPL
                     NPEVIRIWKEGTGLTIREGYGQTETCCCIATMPGMEVKQGSMGKPVPGWHIQLHDDDG
                     NEVPQGETGRIAISLDPRPAGLIREYLDNPEENAAMFVNGWYYTGDKAYKDEDGYFWF
                     VGRNDDVIKSSGYRISPFEVESTLLEHPAVKESAVVGSPDSIRGMVIKAFIVLHDGYQ
                     PSEKLVKDIQDYVKRTTAPYKYPRQIEFVTELPTTISGKIKRAELRNRELQRFAEENG
                     EN"
     gene            2617..3183
                     /locus_tag="O0X37_05090"
     CDS             2617..3183
                     /locus_tag="O0X37_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TATA-box-binding protein"
                     /protein_id="HJK62329.1"
                     /translation="MDGKTYDSLKIENIVASGVIADEINLAEIAEKIDGCELNTKRFP
                     GAVYRIDDPRMASLIFSSGKVVLTGIRNESALQDGLEIILKSLKEAGVKTLDVPKVAV
                     TNIVCSYDIGKFINLNRVVATLSLEAIEYEPEQFPGLVYRIKDPKIVALLFSSGKIIL
                     TGGKNLDDVRRGLDFLEERLDTILKEQK"
     gene            complement(3274..>4123)
                     /locus_tag="O0X37_05095"
     CDS             complement(3274..>4123)
                     /locus_tag="O0X37_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJK62330.1"
                     /translation="IATNAYRILLLLPKFLRAPDVDEVLCSLEPEHIEAILILALKKT
                     ALYKWKLVQVAKKFGAIDADADYEKISISRLLELFDGTVIEKEAFRELFFSNMDVEHA
                     RLVIQQIKDHKMETTTSRLSIIGAEGLFTGRDVIVPPGEDQAIIASVKHRIEEQDIVL
                     ACMHCKNWKSHTKVGRAPDQPQCPVCGARLIAVLKPYDEKMYLAMKKKNKSTEEREAE
                     ARMLRNANMVLSSGKKAIIALAGRGVGPEAAARILNTFATGDNFYREILKAERKFVQT
                     HRFWSD"
BASE COUNT          989 a         1053 c          962 g         1119 t
ORIGIN      
        1 cgcatagact cctccatact ttaacacctg gtggaagtcc gcgacgaatg agcccgagta
       61 ccggttcttg ccgcctgctc cttctacatc agagatgaac tttgcatgtt ccggcagcca
      121 ctctgttctc agacccccgg atccatagag gtttccttcc ggcatcttga cattgtctga
      181 aagcagctga tacacgccgt cactgtcctg agcaaagatt gcaactccat gaccaaccga
      241 aagtgtaagc gtcgtcattg gcccgtagag aatatagaat gcggctttca gattctttcc
      301 aggttgaagt acatccccgt cgtaaatgcc tacaattgta ccaacacaga ggttcactga
      361 gataagggag gagccatcca gcggatccat caccaccgag tactttgccg atgatgagaa
      421 tgtagtgatg tcgtcctgct cttctgatgc gaccgcacgt accaggccgc tttttgcgag
      481 aacacgggtg atatgtgagt ctgcccacgt atccattgcg gcctgttgct ctccggaggc
      541 attttttgag tcagcataat tctgattact gataaacgcc gcacgtattg ggcctgcctg
      601 atcagctatc agtgaaatta ttttactgag atcttcatat acgcccgagt ttttgagata
      661 ttcctgtaga gtcgtcatgc gtattcactg aatatatatt ggacgtgacg atagataagg
      721 gattcatcgc aagttttacc gaggcatgcg atgcagtgtc acttactgct tgaaaaaaga
      781 aaaattagtt ctcaccgttt tcttcagcaa accgctgaag ttcacggttc cgcagttcgg
      841 cgcgcttaat ctttccagag atggttgtcg gcagttctgt aacaaactca atctgccgcg
      901 gatacttgta tggtgcggta gtgcgcttca cataatcctg aatatccttc accagtttct
      961 cggatggctg atatccatcg tgcaggacga tgaatgcttt aatcaccatg ccgcggatgg
     1021 aatcggggct tccaaccaca gcactctctt tgacggcggg gtgttccaga agtgtagact
     1081 ccacttcaaa tggactaatc cggtagccgg aacttttgat cacatcatcg tttcttccga
     1141 caaaccagaa gtatccatcc tcgtccttgt acgccttgtc accggtgtag taccagccgt
     1201 tgacaaacat tgctgcgttc tcctcagggt tatcgaggta ttcgcggata agaccagctg
     1261 gtcttggatc caatgagata gcaatccttc cggtctctcc ctgcgggact tcgttaccgt
     1321 catcgtcatg aagctggatg tgccaaccgg gaaccggttt tcccattgaa ccctgtttga
     1381 cctccatgcc tggcatcgtt gcgatacagc agcaggtctc ggtctggccg tatccttcgc
     1441 gaattgtaag gccggtacct tccttccaga tacggattac ttcaggattc aatggttctc
     1501 ctgcactggt acaggtccgc agctcgctaa acgagaactt cttcagatct gcgatgatca
     1561 acatacggta gattgtcggc ggtgcacaaa acgatgtaat tccgtaacgt tcaatcagtg
     1621 gaagaagctc ggttgcatgg aacttcgtac gataatcata gacgaaaata cacgctccgc
     1681 agatccactg accgtagatc ttaccccagc cgcattttgc ccatccggta tccgataccg
     1741 taaagtgtac atcatattcc gttagatcat gccagaactt tgctgtaacc gtctgaccca
     1801 ggggatggcc atagtcgtgc agcaccatct ttggatcttt ggtggttccg gaggtgaagt
     1861 aaatcagcat cggatcggtc gaaagcattt tgcggccgac actgctgaca agtttcgttg
     1921 agaccggtgc aggatacagc agctcatttt ggtagccgat ccatggggtt ggcaatcgtt
     1981 cgcccggctt atcgtccacc agcatacgga gctggaggtt cggacagtca gctgcgacct
     2041 catcgacctt gtccatgtta tcgctgtcgg tgataaccat cttgaagttg cctaccttga
     2101 tacgataggc gatatccttc accgtcagca tatggggcga gggacaaaac actgcaccca
     2161 gcttcataat gccgagaacc aaaatccacc attccggaac gcgcggaagc atcaggagaa
     2221 ctctgtctcc tttctgaatt ccgaacttca tcagaatatt tgcggcctcg tttgagtgaa
     2281 tcatcatgtc ccagaaggtg aacttctttt caacacccag ctggttgacc cagatcatcg
     2341 ccaagtggtt gcgatccttt ttggcccagg catcgactac atcaaacgcg aagttgtaat
     2401 actttggcgt aacaagggag aacgtacggt acgtctcgtc atagttgcca atgttgcccc
     2461 cgatttttgg ggaaccttgc gtctctgtca tggttcttgt caatattaat gggatgtctg
     2521 aggtaattaa tccgagtgga tatgaaagtc aagctccgtt tgggatgatt caccggacac
     2581 gaagtattat gcttgagtct ctcgtattaa atagagatgg atgggaaaac atacgattcg
     2641 ctgaagatcg aaaatatcgt tgcgtccggc gtaatcgcag atgagattaa cctggcagag
     2701 attgccgaaa agatcgacgg ctgtgaactg aatacaaaac ggttccccgg tgccgtatat
     2761 cgtattgatg acccgagaat ggcttcgtta atcttctcct cgggaaaagt ggtcttaacc
     2821 ggtatcagga atgaatctgc gctccaggat ggacttgaga tcattctgaa atctctcaaa
     2881 gaagcaggag tcaaaaccct ggacgtcccc aaggttgcag tgaccaacat tgtctgctcc
     2941 tatgacattg gtaaatttat taacctgaac cgtgttgtgg caacactgtc tcttgaggca
     3001 attgagtatg agccggaaca gttcccaggt ctcgtgtacc gtatcaagga cccgaagatt
     3061 gttgcgctcc tcttctcctc aggaaagatc atcctgaccg gcggaaagaa tcttgatgat
     3121 gttcgccgtg gtcttgattt ccttgaagag cgtctggaca ccatcttaaa agaacagaaa
     3181 taaggcagag gatgctgccc gtacctcctg ggtgcatccc cgaaatccgg gttgtttcat
     3241 caacatatcc ccgaatcttt ttttcagaat ttttcaatca gaccagaacc gatgcgtctg
     3301 tacaaacttt ctttcggctt ttagaatttc tcgataaaag ttgtcaccgg ttgcaaatgt
     3361 gtttaagatc cgtgccgcag cctccggtcc caccccccgt cctgcaagtg cgataatggc
     3421 ctttttccca cttgagagta ccatatttgc attgcgtagc atccgtgcct ctgcctcacg
     3481 ctcctcagta cttttgttct tctttttcat cgccaggtac atcttttcat catagggttt
     3541 gaggacagca atcagtcgtg ctccgcatac aggacactga ggctgatccg gggcgcgccc
     3601 tactttggta tggcttttcc agtttttgca gtgcatacat gcaagcacaa tgtcctgttc
     3661 ttcaatccgg tgtttcacac ttgcaatgat cgcctgatcc tctccgggag gcacaattac
     3721 gtctcttccg gtaaacaatc cctctgctcc gataatggac aggcggcttg ttgttgtctc
     3781 cattttgtgg tctttgatct gctgtattac aagacgcgcg tgttccacgt ccatgttcga
     3841 gaagaacagt tcacggaatg cctccttctc aatcaccgtt ccgtcaaaca gctccagcaa
     3901 acggctgata ctgatctttt catagtcggc atccgcatca attgcgccga acttttttgc
     3961 cacctgcacc agtttccatt tgtacagagc cgtctttttg agggcaagta taagaatcgc
     4021 ctcaatatgt tccggctcaa gagaacagag tacttcatcc acatccgggg cacgcaaaaa
     4081 ttttggcagc agcaaaagaa tccggtaggc attggtcgca ata
//
LOCUS       DALUAJ010000243         2307 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_48311,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000243 DALUAJ010000000
VERSION     DALUAJ010000243.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48311"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="O0X37_05100"
     CDS             <1..203
                     /locus_tag="O0X37_05100"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HJK62331.1"
                     /translation="VIGAVNAVSSADMALAGIVSRIPPDQVIDAMKEVGESMHISLKE
                     TGEGGVANTPEARKIKFNLNLQ"
     gene            296..1396
                     /locus_tag="O0X37_05105"
     CDS             296..1396
                     /locus_tag="O0X37_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HJK62332.1"
                     /translation="MKRRIISALLLVCITAAIASGCGSDEPVETVDLNSMTLEEIEAQ
                     AKEEGELASAAMPDTWANWGETWQEYTDTYGIEHVDTDMSSAEEISLFETEGTDATKD
                     IGDVGQAFGPVAEEQGVTLPYKTSYWDSIPDWAKDDDGDWIIGYYGTMSVIVNNKIVK
                     DTPTSFEDILNGDYIVAVGDVQAATQAQYAVLAAAIAYGGDESNIQPGLDYFRQIAEQ
                     GRLDLGEFTQARIEKGEVGVAFLWDFNALGYREQFVENNPDADFTVFIPSEASIQTGY
                     ATIINKYSKHPCAAALAREYILSDEGQINLAKGYATPIREDVELPDDVASKLLDDSQY
                     ANARMVEDQDAWDETAQTIGTLWQEEGGAYAQ"
     gene            1591..>2307
                     /locus_tag="O0X37_05110"
     CDS             1591..>2307
                     /locus_tag="O0X37_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HJK62333.1"
                     /translation="MGFTLDNYINIFTGKLYQQAIVNSLLISILSSVIGLLVAFIGAK
                     AVHEEKGKLKQVFMSILNMVSNFSGVPLAFAYIIMFGNIGVMTMIGANYGIEFLAEFP
                     LYSVWGLLLIYVYFQIPLSTLLLIPAFDSIRKEWKEAVALLGGSRMTFWRKVGIPVLM
                     PSILGTFSVLFANALAAYASAYALLMNNVSLLPIRLSEQFVGDIIQRPEFGSAIAVIM
                     MVFMVIAIGVQNKMTPKKGGR"
BASE COUNT          659 a          436 c          617 g          595 t
ORIGIN      
        1 atgtgatagg tgcggttaat gccgtttcaa gcgcagatat ggctcttgcc ggaattgtaa
       61 gtagaatccc gccggatcag gtcatcgatg cgatgaaaga agtaggggag agtatgcata
      121 tttctttaaa agagacaggg gaaggcggag tggccaatac tccggaggca agaaaaataa
      181 aatttaacct gaacttacaa tgacttaacg ttccgttggt gattatttta catcattttc
      241 ctataatgta tctcggtaat tcaaaagaaa agaagttgaa aaagggagaa caggaatgaa
      301 aagaagaata ataagtgcgt tgctgcttgt ctgtattact gcggcaatcg cttcaggatg
      361 cggttctgat gaaccggttg aaacagttga tctgaacagt atgactctgg aagagattga
      421 agcacaggca aaagaagagg gagaacttgc atctgcggcg atgcctgata catgggccaa
      481 ctggggtgaa acatggcagg agtacacaga tacgtacggg attgagcatg tggatactga
      541 tatgtcttct gcggaagaaa tttcgctgtt tgagacagag gggacagatg ctacgaagga
      601 tattggtgat gtaggacagg cattcggacc ggtagcggaa gagcagggag ttacacttcc
      661 gtataagaca agctactggg attcgattcc ggactgggcg aaagacgatg acggagactg
      721 gatcatagga tattacggca caatgtctgt tattgttaac aacaagatcg taaaagacac
      781 acctacatct tttgaagata tcttaaacgg tgattatata gttgcagtcg gagacgtgca
      841 ggctgccact caggctcagt atgcagtcct tgctgcagca attgcttacg gcggagatga
      901 aagcaacatt cagccgggac ttgattactt cagacagatt gctgagcagg gaagacttga
      961 cctcggcgag tttactcagg cacgtatcga gaaaggagaa gtcggagtag ctttcttatg
     1021 ggatttcaac gctcttggtt acagagagca gtttgtagag aataatccgg atgcagactt
     1081 tacagttttt atcccgtctg aggcatctat ccagaccgga tatgctacaa ttatcaacaa
     1141 atattcaaag catccgtgtg ctgcggctct tgcgagagaa tacattttaa gtgatgaggg
     1201 acagatcaat cttgcaaaag gttatgcaac accgatacgt gaggatgtgg agcttccgga
     1261 cgatgtggct tcaaaactcc tggacgacag ccagtatgca aacgcgagaa tggtagaaga
     1321 tcaggatgca tgggacgaga ccgcgcagac gatcggaaca ttgtggcagg aagagggtgg
     1381 agcttacgca caataaaatg tatttacgga ttagcggact gctgcataaa gtgcaacggt
     1441 ccgttttatg taaaaggtga gggtaatatg aaaacacaaa agaaaacata catatttgcg
     1501 ctggtgccgt ttttgctgct atgtcttctg tttgagatca taccggtcat ttttacggtc
     1561 atcagaagtt tcttcccgga gggaggagac atgggattta cacttgataa ctatattaat
     1621 atatttaccg gaaaactgta tcagcaggcc atagtcaaca gtctgctcat ctcgattctt
     1681 tcttcggtta tcgggctgct cgtggcattt atcggtgcaa aagcagtcca tgaggaaaaa
     1741 ggtaaactga agcaggtatt catgagtatt ctgaatatgg tatcaaattt ttcgggcgta
     1801 ccgctggcgt ttgcatatat cattatgttt ggaaacatcg gtgtcatgac gatgatcggc
     1861 gcgaattacg gaattgaatt tctggcggag ttccccctgt attctgtatg gggacttctg
     1921 ctgatatatg tatatttcca aatccccctg tccacactcc tgctgatccc ggcatttgat
     1981 tcgatcagaa aagagtggaa agaagcggtt gcccttctgg gcggcagccg gatgacattc
     2041 tggaggaaag tcggcatccc agtactgatg ccgagtatac tgggaacatt ttctgtattg
     2101 tttgccaacg cactggcagc ttatgcgtct gcctatgcac tgctgatgaa caatgtatca
     2161 ctgcttccga tccgtctgtc ggaacagttc gtcggcgata tcatacagag accggagttc
     2221 ggaagtgcga ttgcagtcat catgatggtc ttcatggtga tcgccattgg cgtacagaat
     2281 aagatgactc caaagaaagg ggggaga
//
LOCUS       DALUAJ010000244         3335 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_48470,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000244 DALUAJ010000000
VERSION     DALUAJ010000244.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48470"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(241..666)
                     /locus_tag="O0X37_05115"
     CDS             complement(241..666)
                     /locus_tag="O0X37_05115"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin"
                     /protein_id="HJK62334.1"
                     /translation="MKFHADRDEAVSPVIATILLVALTVVLVAIVAVVMVGMANDIVP
                     TKTVAATVLAGDGYATATVVSGNDVHQLTNFSLTLFNDGGTYTNLMDEGTGVELVGKI
                     LRVPASGKVSSGIYQYTITGNFSDGSSQVLSIGTITLTA"
     gene            809..1057
                     /locus_tag="O0X37_05120"
     CDS             809..1057
                     /locus_tag="O0X37_05120"
                     /inference="COORDINATES: protein motif:HMM:NF019085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HJK62335.1"
                     /translation="MIETILMVIVAVAIAAILFFLLRNGLKLLINAVCGVVILFIVSY
                     FNLAPIGDLSIAQVIVCAVGGILGAALLIILSFFGIII"
     gene            complement(1148..3073)
                     /locus_tag="O0X37_05125"
     CDS             complement(1148..3073)
                     /locus_tag="O0X37_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HJK62336.1"
                     /translation="MTSTPPSLKDRKKMRLAVLEAEIFSARMQVKAEKLYLYALISGC
                     LFGTVLYLFLLYLSLRMVPPSLFLNAPEFCVIFGVTLLVPAFAAGYVVFAGVLYLPKV
                     LCKLRGTRIDLSLFHAVTYLYALHQSEPNLYAVVESLAKYAASYGEAAREFRQVISDC
                     RNCSLDFYSALGRLSQTTPSEKFRSFLTGLSSSYRTLGSATEYLRMKTDELREEQRLS
                     QKMYLNTLGVIAEIYITVFVAGPLFLVIVVMVMGVISSVNPLVLALIIYVMLPFGTAL
                     FLLILSVISDTHEETTTEIMHAEQSEEIRKQVFSSVRITIPQVSEAPAFEKLARRDAR
                     EKYLRFFHAPVSYMKFHPPAVFACSVPAALLLMAVLYLLFVPVPLTPDGFLSWIAAAE
                     NVVIAGVLIALVPFAVFYEIQTRYRRRVEGAVPDFVDQLVSAVRHNLTLSQAIDLIVL
                     EGRSDMLEEIRMVRRDILWGSRTSDAVRRFRENVPVGVIDRMSVLLMQAEHFTDNLTQ
                     VLQIIAEDSKSLVMRKNERRGEMQLYVIVVYLAFFVFVFVQSIMAVMFLPMLLQSGSG
                     LLVIGSGVTNTVTGWSPADIYNCLIYHSVLIHGFCSGLVAGMMGEGSVCAGIKHSCVM
                     IVVAVVVFTFVKFFLLT"
     gene            complement(3070..>3335)
                     /locus_tag="O0X37_05130"
     CDS             complement(3070..>3335)
                     /locus_tag="O0X37_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62337.1"
                     /translation="YNRIAEISGNDVNWVVREQQRRAVVLTAMVEQQIRDYRDVSEIL
                     WRYAAAPELVLSSLDDLTVLLEHGSVTPRPRRNMKADVVPPSV"
BASE COUNT          938 a          872 c          758 g          767 t
ORIGIN      
        1 gggctgcagt gatcatggat ttacattggg cttcggtttc tttgatgctt ttgagaacct
       61 cagttttcat ccaaccctcc tctcacggca acataatatt aggtgagcat tagtgtttat
      121 acttacgttc tttggttgca gcaggatgtg aggaaagggg gtgagaggtc ttcagaacaa
      181 ggggttcaga gatgcaggaa agaacgagcg caggacacga aatggatcaa aaaagaaggg
      241 ttacgcagtc agcgtaatgg ttccgatgga cagcacctgg ctggaaccgt cggagaagtt
      301 tccggtgatg gtgtactgat atatccccga actgactttt ccggatgcag gaacacggag
      361 gattttccca acgagttcca ctcccgtacc ttcatccatg agatttgtgt aggtaccgcc
      421 gtcgttgaac aaggtaagac tgaagttggt gagctgatga acgtcgtttc cgctgaccac
      481 agttgcagtg gcatacccgt caccggcaag tactgtcgca gcaacggttt tggtcgggac
      541 gatgtcattt gccattccca ccatgacaac agcgacaatg gcaacgagta caacggtgag
      601 agcaaccaga agaatagtgg caatgaccgg agacaccgcc tcgtctctgt ccgcatgaaa
      661 tttcatgatg atgtgtaatt gcgaatcaga ctgatataaa atatatgtcc ggaacaaaaa
      721 cctcagaact aaatgagagg aattccccgc cagagattcc gattaccaca atattttttt
      781 cctgcatcgt ccatatgagt aattaagcat gatagaaacc attctgatgg tcattgtcgc
      841 tgttgcaatc gcggcaatcc tcttcttcct cctccggaac ggactgaaac tcctcatcaa
      901 tgcagtgtgc ggagtggtta tcctctttat tgtctcgtac tttaacctcg cccctatcgg
      961 tgatctttcc attgcccagg ttattgtctg tgccgttggc ggaatacttg gcgcagcact
     1021 gctgatcatc ctttccttct ttggaatcat catctaacaa tctctttttc atctctttcg
     1081 gcagcctaaa ggcaaggaac ggatcaggtt ccggacggaa aacagaacca gagacgaaca
     1141 cgatcactta cgtcagaagg aaaaatttta caaacgtaaa caccaccact gcaaccacta
     1201 tcatcacaca cgagtgtttg attcctgcac agaccgagcc ttcacccatc atccctgcaa
     1261 caagtccgga acagaacccg tgaatgagta ctgaatgata aatcagacaa ttatagatgt
     1321 cagctgggga ccatccggtt acagtatttg tcactccgga acctattaca agaagaccgc
     1381 ttccactttg cagcagcatc ggcaaaaaca tcaccgccat aatgctctgc acaaagacaa
     1441 acacaaaaaa tgccagatac accacgatca catacaactg catctctccc cggcgttcgt
     1501 ttttcctcat caccagactc tttgagtcct cggcaatgat ctggagaacc tgtgttaaat
     1561 tgtctgtaaa atgttcagcc tgcatcagca gaacactcat ccgatcgatc actcccaccg
     1621 gcacattctc acgaaacctc cttaccgcat ccgatgttct gctgccccac agaatatcac
     1681 ggcgaaccat ccgtatctcc tccagcatat cactccgtcc ctcaagcaca atcagatcga
     1741 ttgcctgtga aagcgtcaga ttatgccgga cagcagagac cagctgatct acaaaatcgg
     1801 gaaccgcacc ttctactctt cgccggtacc gggtttgtat ctcataaaat accgcaaacg
     1861 gtacgagtgc gataaggact cccgcaatca caacattctc cgctgccgca atccacgaaa
     1921 gaaacccatc aggtgttagt ggaacaggta caaacaggag atacaggaca gccatcagca
     1981 aaagtgctgc aggaacactg catgcaaaca ccgccggtgg atgaaacttc atataagaaa
     2041 caggggcgtg gaaaaaccgc agatactttt cccgtgcgtc acgcctggca agtttctcaa
     2101 acgccggagc ctccgagacc tgcggaatcg ttatgcgaac agaagagaac acctgtttcc
     2161 ttatctcttc actctgttct gcatgcatga tctctgttgt ggtttcctcg tgtgtatctg
     2221 agatgactga aagaatcaac aggaataacg cagtcccaaa cggtaacatc acataaatga
     2281 tcagagcaag gaccagcggg tttacacttg agataactcc catcaccatg acaacgatta
     2341 caagaaagag ggggcctgca acaaaaactg tgatgtagat ctctgcaata actcccagtg
     2401 tgttcaggta catcttctgg gagagccgct gctcttcccg cagttcatcc gttttcatcc
     2461 gaagatactc ggttgcggaa ccgagtgtac ggtatgatga cgacaaaccc gttagaaagg
     2521 accggaactt ctcagacggt gtggtctggg aaagtctccc aagcgctgag taaaaatcca
     2581 gggaacagtt ccggcagtca ctgatgacct gccggaactc gcgtgccgcc tcaccatacg
     2641 aggcggcgta ttttgcaagt gattccacca cagcatacag attcggctcg gactgatgca
     2701 gtgcataaag atacgttacc gcatgaaaca gcgagaggtc aatacgtgtc cccctgagtt
     2761 tacacagaac ttttggcaga tacaagaccc cagcaaacac cacgtatccc gccgcaaacg
     2821 caggcacgag aagtgtgaca ccgaaaatca cacaaaattc cggagcgttt aggaataatg
     2881 acggcggaac catccgcagg gagagatata aaagaaaaag atacagaacg gttccgaaca
     2941 aacatcccga aatcaatgca tacagataca gcttttctgc ttttacctgc attcgtgccg
     3001 aaaagatctc tgcttcaaga actgccagac gcatcttctt tcgatctttc agagagggag
     3061 gggtactcgt catacggatg ggggaacaac atcggctttc atattccggc gcggacgcgg
     3121 tgtcacactg ccgtgttcaa gaaggactgt gagatcatcc aatgaagaaa gaaccagctc
     3181 cggtgctgct gcatacctcc agagaatctc cgacacatcg cggtagtcgc ggatctgctg
     3241 ttcaaccatc gccgtgagaa caactgctct gcgctgctgc tctcggacaa cccagttcac
     3301 atcattcccg gagatctcag cgatgcggtt ataga
//
LOCUS       DALUAJ010000245         2586 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_48579,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000245 DALUAJ010000000
VERSION     DALUAJ010000245.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48579"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(111..362)
                     /locus_tag="O0X37_05135"
     CDS             complement(111..362)
                     /locus_tag="O0X37_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HJK62338.1"
                     /translation="MTIETMTVEAAQAMASGYTAIGAGLAVGLAGVGTGLGEMGIGAA
                     AVGATAEDRDMFGLALLFTVLPETIVIFGLVISLLLLFQ"
     gene            complement(368..2374)
                     /locus_tag="O0X37_05140"
     CDS             complement(368..2374)
                     /locus_tag="O0X37_05140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HJK62339.1"
                     /translation="MFQPKPMTHLLIAAPKEQMASVVTELYRHRVFHITDFVDQGKEG
                     YEGVRLGTPLEGAGELSTSLLKIRSIENVFQTSPETLFDVPKRPVATIRQAIERELPV
                     IEAEVNDLTVQRSAAESRIRECEQRITELKPFALVPLEMDLYHGYESISVLAGYIAKD
                     VEISVPHEKYYAARKDGNFIVVFAETKDVAEIERILADSGFQSVTIPNETGSVQAAVD
                     KYTADQSQAKAAFDDANAKISELKAKYADLLAACDEVLSGDVERAEAPLRFATTEEAF
                     VIDGWVPADHIEPITAGLNQATGGRVYVTVDDDEIDATAIPVEYNNPSFAKPTELFMD
                     VYARPRYNEIDPTLILAIMFPIFFGLIVGDVGYGILFLILALFARKLAMFKGEGGKNL
                     IKILVGSSISTIFFGLLYSECLGYSLPWHAIIFQRHLAIGADAAAHASGPDAMPLLIV
                     SVWFGIIYITLGRIFGMINHYRMDHAGKHRTKAILGQFGWIAILWGLLVLVWSFFSME
                     QMFGIVGMMPDLTTAPMIVPGFSVAALAGLILLVVGCILLAQENVLDLMEIPTIISHV
                     LSFCRIAAVGLSSVAIAMVVNYLSFGMFIDPALANLDAVGVIMIIVGVLIFICGHALN
                     VGLGILGGGLHSIRLHYVEFFTKFYVGGGIKYNPFGLKRKFSEE"
     gene            complement(2361..>2586)
                     /locus_tag="O0X37_05145"
     CDS             complement(2361..>2586)
                     /locus_tag="O0X37_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HJK62340.1"
                     /translation="LEADNLIAKATSVAEDYKKQRLSDARNVAAAKHAAIVEQGKADA
                     DSTIAQGSKKLPQAASLFVERFKEKLHVSA"
BASE COUNT          626 a          632 c          675 g          653 t
ORIGIN      
        1 ctttgcttca gcttccgcct cggatttgat ccgggcagcc tcgcgttcac cctttgcctt
       61 gatttcgtca actacaacct caagtcccat gatttgcacg agcctccgct ttactggaag
      121 agcagcagca gggaaatcac aagaccgaag atgacgatag tctccggaag cacagtgaag
      181 agaagggcaa gaccgaacat gtcacggtct tctgcggttg ctccgactgc ggcagctccg
      241 atacccattt caccaagacc ggttccgacg ccggcgagac cgactgcgag acctgcacca
      301 attgcggtgt atccggatgc cattgcctgt gctgcttcaa ctgtcattgt ttcaattgtc
      361 ataattatta ctcctctgag aatttgcgtt taagtccaaa cggattgtac ttgatgcctc
      421 caccaacgta gaacttggtg aagaattcaa cgtagtgaag acgaatcgag tggagacctc
      481 caccaaggat accaagacct acgttgaggg cgtgtccgca tatgaagatg agaacaccaa
      541 caattatcat tattacaccg actgcatcca ggtttgcaag ggctgggtcg ataaacatac
      601 caaacgagag gtagttgacc accattgcaa ttgcaacgga tgaaagtcca actgcagcaa
      661 tacggcagaa ggaaagcacg tgcgagatga tagtcgggat ttccattaag tcgagcacat
      721 tctcctgtgc aaggaggata catccgacga cgagcagaat gagtcctgca agagctgcta
      781 ccgagaatcc agggacaatc atgggtgcag tggtcaggtc aggcatcatg ccgacaatgc
      841 caaacatctg ttccattgag aagaaggacc agacgaggac taagagaccc cagaggatgg
      901 cgatccatcc aaactgtccg agaattgctt tggtccggtg ttttccggca tgatccattc
      961 tgtaatggtt gatcattcca aagatgcggc caagcgtgat gtagatgata ccaaaccaga
     1021 ctgatacaat cagtaagggc attgcatccg gtccgcttgc gtgggcggcg gcatctgctc
     1081 cgatggctaa gtgacgctgg aaaatgatag cgtgccacgg cagggaatat cctaagcact
     1141 cgctgtacag gaggccgaag aatattgtgg agatgctaga gccgacgaga attttgataa
     1201 ggtttttccc gccttcgcct ttgaacattg ccagtttcct ggcaaagagt gcaaggataa
     1261 ggaacaggat accgtatccg acatcaccaa caatcagtcc gaaaaagatc gggaacatga
     1321 tggcgaggat cagtgtcggg tcaatttcgt tgtatcttgg acgtgcataa acgtccatga
     1381 aaagttcagt gggctttgcg aacgatgggt tgttgtactc caccggaatt gctgttgcgt
     1441 cgatctcatc gtcgtcaaca gtcacatata cgcggccacc ggttgcctgg tttaagcctg
     1501 cggtaatcgg ttcgatatga tctgccggaa cccatccgtc gataacgaat gcttcctcag
     1561 ttgttgcgaa ccgtaacggg gcttctgcac gttccacatc gccggacagt acttcgtcgc
     1621 atgcggcgag taagtcggcg tattttgctt tgagctcgga aatttttgca tttgcatcat
     1681 caaaggcagc ttttgcctgg ctctggtcgg cagtgtactt gtccactgcg gcctgaacac
     1741 taccggtctc gttcggaatc gtaacggact ggaagcctga gtcggctaat atgcgttcga
     1801 tttctgcgac atcttttgtt tcggcgaata caacgatgaa gttgccgtct ttgcgggccg
     1861 catagtattt ttcatgcggg acgctgatct cgacatcttt ggcgatatat cctgcgagaa
     1921 cagagatgct ttcgtaaccg tggtagagat ccatctcaag cggtacgagt gcaaaaggtt
     1981 tgagttcagt aatgcgctgt tcacattccc ggatgcggga ttctgcagcc gatcgttgta
     2041 ccgtgaggtc gttaacttct gcttcgatta cgggaagttc acgctcaatt gcttgtcgga
     2101 tcgttgctac aggacgcttc gggacgtcga agagcgtttc agggcttgtc tggaatacgt
     2161 tttcaatgga acgtatctta aggagactgg tcgagagttc accggcccct tcgagagggg
     2221 tacctaagcg aacaccttca tagccttctt ttccctgatc tacaaaatcg gtgatgtgga
     2281 agacccggtg acggtatagt tcagtcacaa ctgacgccat ctgctcttta ggcgctgcaa
     2341 tgagcagatg cgtcatcggc ttaggctgaa acatgcagtt tctccttaaa ccgttctaca
     2401 aacagcgatg ctgcctgagg gagttttttg cttccctgtg caatggtgga gtcggcgtct
     2461 gctttgcctt gttccacaat tgcggcatgt tttgctgctg caacatttcg tgcatctgaa
     2521 agacgctgct tcttgtaatc ctcggccact gatgttgcct tggcaatcag gttatcagct
     2581 tcaagc
//
LOCUS       DALUAJ010000246         2915 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_48670,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000246 DALUAJ010000000
VERSION     DALUAJ010000246.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48670"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            33..1358
                     /locus_tag="O0X37_05150"
     CDS             33..1358
                     /locus_tag="O0X37_05150"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HJK62341.1"
                     /translation="MFPIDGRTTLTVDDIPKKWYNIAADIGSPEILNPATMKPVTAED
                     LAPVFCKTSVEQELDTTHRFIDIPEPVREAYAQFGRVTPLQRAYHLEQYLKTPAKIYF
                     KREDVSPTGSHKLNSAIAQAYYNKKDGVEHLTTETGAGQWGSALSLSCNYFDLDLIVF
                     MVKVSFEQKPFRRSIMETYGGTVYPSPSNVTAYGRSVLAKDPSYGGTLGTAITEAVEM
                     AVKDQTGKTKYSLGSVANHVCMHQTVIGQELIAQMDELDIVPDYMVGCIGGGSNFAGF
                     VFPMIEKKLKGKVDTTFLAVEPSDVPSLTKGEFRYDYGDAGCITPMFKMYTLGHDFTP
                     NALHAGGLRYHGMNPLVSELYHNKAISAVSYNETQIFQAAVTFARTEGIIPAPESSHA
                     IRGAIELALDAKKKNEEKTIIFNLSGHGLLDMVGYGNFLKNCDHPMIDV"
     gene            complement(1355..1999)
                     /locus_tag="O0X37_05155"
     CDS             complement(1355..1999)
                     /locus_tag="O0X37_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011844852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HJK62342.1"
                     /translation="MRVKICGTATFADLDCAVAAGADAVGFLMGITHVTQDAVTPETA
                     AAMVATLPPFIVPVAVTHLTKPSDLIRIVELARCTTLQIQDVVTPDDIAEVREALPYL
                     RIMKAVHVMGESAIATAKYFSDTADAILLDTRTADRIGGTGITHDWNISAKIVKECSC
                     PVILAGGLTPENVTEAIIRVRPYAVDVHTGVKKNGVRDAERTRAFVANARIARD"
     gene            complement(1990..>2915)
                     /locus_tag="O0X37_05160"
     CDS             complement(1990..>2915)
                     /locus_tag="O0X37_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012619229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HJK62343.1"
                     /translation="TKEQWGLRVPVQDGESARRTAIAENILDRTLRPRAENGYLLIPV
                     VSPRDGELRAEFAENHVIEELPRHEQIGGIVVLQDDDPAGAEKILAARPSAHTALFAT
                     SPVEGEFRTKTFKVLAGVPTTETMYHEYGRRMVIDLTAAYFSARLANERQRILSQMRG
                     DETVLDMFAGVGPFPVMLGGSASFVIANDINPGAVLLMQKNIRLNHLTNVVPMLGDAR
                     NLARILAPMKFDRIIMNLPMDAAEFLPEAAKLAKPGTIVHLYSLVEREDEHTADILHV
                     FPGAKITERVLRSYSPTSWHAVYDIEVAACE"
BASE COUNT          754 a          818 c          666 g          677 t
ORIGIN      
        1 attatgaata cctctaagta acaccttgat ctatgtttcc aatagatggt cgtacgaccc
       61 ttaccgtgga tgatattccc aagaaatggt ataacattgc cgcagatatt ggttcgccag
      121 aaatactcaa ccccgcaaca atgaaaccgg taactgccga ggatctggca cccgtattct
      181 gcaaaacctc tgtagagcag gaacttgata caactcaccg gtttattgac atcccggaac
      241 ccgttcgtga ggcttatgcg cagtttggca gggttacacc actgcagcgt gcttatcacc
      301 ttgaacagta cctcaaaacc ccggcaaaaa tttactttaa acgtgaagat gtaagcccca
      361 ccggcagcca caaactgaac agtgctattg cgcaggcata ctacaacaaa aaggatggag
      421 ttgaacatct tactaccgaa accggagccg gtcagtgggg atcagcactt tccctctcct
      481 gcaactactt cgatctcgac ttaatcgtct tcatggtcaa ggtcagtttc gaacagaaac
      541 ccttccgccg atccatcatg gagacctatg gaggaacagt ctatccctct ccgagcaatg
      601 taactgcgta tggaagatct gttcttgcca aagatccttc atacggagga accctcggaa
      661 ccgctatcac tgaagcagtc gaaatggcag tcaaagatca gaccggtaaa accaagtaca
      721 gtcttggcag tgttgcgaac catgtctgca tgcatcagac ggtgatcggt caggaactca
      781 ttgcccagat ggatgaactc gacatcgttc ccgactacat ggttggctgc attggaggcg
      841 gaagtaactt cgcagggttc gtcttcccca tgatcgaaaa gaaactcaaa ggaaaagtag
      901 acaccacatt ccttgcggtc gaaccaagtg atgttccctc tctcaccaaa ggagaattcc
      961 gctatgacta cggtgatgcc ggatgtatca cgccgatgtt taagatgtac actctcggtc
     1021 acgactttac tcctaatgca ctccatgcag gcggccttcg gtatcacggc atgaacccgc
     1081 tggtctcaga gctctaccac aacaaagcca tcagtgccgt gtcctacaac gaaacccaga
     1141 tcttccaggc agcagtaacc ttcgccagaa ccgaaggaat catccccgca cccgaatcct
     1201 cacatgcaat ccgcggagca atcgaacttg cacttgacgc aaagaaaaag aatgaagaaa
     1261 aaaccattat cttcaacctc tccggtcacg gacttcttga catggtcggc tacggaaact
     1321 tcctcaaaaa ctgtgaccac ccaatgattg atgtttaatc acgggcaatc ctggcatttg
     1381 caacgaatgc acgggttcgt tccgcatccc gaacaccatt cttttttaca ccagtatgca
     1441 catccacggc atacggacgg acgcggatga tcgcctcggt cacattttcc ggtgtcagac
     1501 ctccggcaag aatcaccggg caggaacatt ccttcacaat ttttgcactg atgttccagt
     1561 catgggtaat gccagtccct ccaatccgat ccgccgtcct tgtatcaagg agaattgcat
     1621 ccgctgtatc cgagaagtac ttcgccgttg caatcgcact ctcacccatc acgtgtacag
     1681 ccttcatgat ccgaagatat ggcagtgcct cgcgaacctc ggcaatatca tccggagtta
     1741 ccacatcctg aatctgcaat gttgtacagc gggccaactc cacaatgcgg atcagatcgg
     1801 acggctttgt cagatgggta actgcaacag gtacaatgaa cggaggaagt gttgcaacca
     1861 tcgctgccgc agtttccgga gtcaccgcgt cctgtgttac atgggtaatt cccatcagaa
     1921 atccaaccgc atccgctcca gctgcaaccg cacagtcaag gtctgcaaac gttgctgtgc
     1981 cgcagatctt cactcgcatg cggcaacctc aatgtcgtac accgcatgcc agctggtcgg
     2041 cgagtacgaa cgaaggaccc gctcggtaat ctttgcgccc gggaacacat ggagaatgtc
     2101 ggctgtgtgc tcgtcttccc gttcaaccag tgagtagaga tgtacaatag ttcccggctt
     2161 cgccagcttt gctgcttcag gcagaaactc cgccgcgtcc atcgggagat tcataatgat
     2221 acggtcgaac ttcatcggtg caagaatcct cgcaaggttt ctggcatcac caagcatggg
     2281 tacaacattg gtcagatgat tgagccggat atttttctgc atcaaaagaa cggcacccgg
     2341 attaatgtcg tttgcgatca caaacgatgc ggacccgcca agcatcactg ggaatggtcc
     2401 cactcctgca aacatgtcaa gaactgtttc gtcaccccgc atctgtgata aaattctctg
     2461 ccgctcgttg gcaagacgtg ccgaaaaata tgcggcggtc agatcgatta ccatgcgccg
     2521 tccgtactcg tggtacattg tttcagttgt tggaacaccc gcaagaacct tgaacgtttt
     2581 tgtacggaac tccccttcca ccggtgatgt agcaaaaaga gctgtgtgtg ccgatgggcg
     2641 tgcggcaaga attttttccg caccggctgg atcatcgtcc tgcaaaacaa caatgccgcc
     2701 gatctgctca tgccgcggta gttcttctat tacatggttt tctgcaaact ctgcccggag
     2761 ttctccatca cggggggaaa caaccggtat cagcagatat ccattttccg cacgggggcg
     2821 aagtgtccga tcaagaatat tttctgcaat tgccgtccgt cttgcggact caccatcctg
     2881 taccggcacg cgaagacccc actgctcctt tgtca
//
LOCUS       DALUAJ010000247         2043 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_48881,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000247 DALUAJ010000000
VERSION     DALUAJ010000247.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48881"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="O0X37_05165"
     CDS             complement(<1..408)
                     /locus_tag="O0X37_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007313935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HJK62344.1"
                     /translation="MNVRIGTRGSKLALAQTNNVLRLLTDAGISAESQIITTSGDAVL
                     DRGLHQIGGQGVFVRELDNAILRGEIDAAVHSMKDIPAERPEGLITAAILPRDPPYDF
                     FVFNCPVEKIFSVGTSSTRRRAQLLRYYHCMPQV"
     gene            complement(405..1646)
                     /locus_tag="O0X37_05170"
     CDS             complement(405..1646)
                     /locus_tag="O0X37_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015285824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HJK62345.1"
                     /translation="MNQNETLFHKAQTLLPGGVSSPVRAIKPYPFYVKSAKGAVLTTV
                     DDTRLVDCCLGYGPLILGHANPVIKAAISSQLDNGWLYGTPTELEIDLAERICNDHPS
                     MDMLRFVSTGTEATMAALRLARGFTGKTDIVKVEGGFHGAHDAVLIAAGSGATTHGTP
                     DSAGVLPDFAAHTRQVPYNDPGALETLLSKNDNIAAFIIEPVLGNIGPVIPDDGYLKA
                     VREITAAHDVLLIFDEVITGYRLGIGGAQVKFDIKPDLTTLGKIAGGGLPIGVFGGKR
                     EIMEMISPAGPVYNAGTFNANPATMAAGIAMNTYLHTHEDIYAKLDEKTRTIEESLPA
                     GASGTFVRLGSMFKYFFRSSAPRNYTEAKQCDTGAFRVFWEKALAAGVFLPPSQFETN
                     FLSAAHGDAEMTKLVQVYGQC"
     gene            complement(1643..>2043)
                     /locus_tag="O0X37_05175"
     CDS             complement(1643..>2043)
                     /locus_tag="O0X37_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HJK62346.1"
                     /translation="SYSTKFASALYGPFREAADSGFSFGDRSTYQMNPANGREAFRES
                     LLDMEEGADILMVKPASFYLDVLAQVRELGLPTAAYQVSGEYSMIKAAAQNGWIDEKR
                     IVMESLTSIKRAGADLIITYYAQDVARWLA"
BASE COUNT          467 a          564 c          552 g          460 t
ORIGIN      
        1 cacctgcggc atacagtgat aataccggag aagctgcgcc cgccggcggg tactcgacgt
       61 tcccaccgag aagatctttt ctaccggaca gttaaacaca aaaaaatcat acggcggatc
      121 acggggaaga attgctgctg tgatcaatcc ctcaggccgt tctgccggga tgtctttcat
      181 gctgtgcacc gctgcatcga tttcgccacg caggattgca ttgtccagtt cgcggacgaa
      241 cacaccctgt ccgccgatct ggtgcaggcc gcggtcaagc accgcgtcac cggacgtggt
      301 gatgatctgc gactctgcag aaatgccggc atcggtgaga agacggagca cattatttgt
      361 ctgtgcaagt gccagtttgc tgccgcgggt gccgatccgt acgttcagca ttgtccgtac
      421 acctgcacaa gttttgtcat ctccgcatca ccgtgggcag cagagaggaa gttcgtctca
      481 aactgtgatg gaggtaagaa cacaccggct gcaagtgctt tttcccagaa aacacggaac
      541 gcaccggtat cacactgctt tgcctcggtg tagttccttg gcgcagatga acggaagaag
      601 tacttgaaca tcgaaccgag ccgaacaaac gttccggatg ccccggctgg aagagactcc
      661 tcgattgtcc gtgttttttc atccagtttt gcgtagatgt cctcatgggt atggagatat
      721 gtgttcatcg caataccggc cgccattgtt gccgggtttg cattgaacgt acctgcattg
      781 tacaccgggc ctgcgggaga gatcatctcc ataatctctc gcttacctcc aaacactcca
      841 atcggcagac cgcctccggc aatttttcca agagtggtca gatccggttt gatgtcaaac
      901 ttcacctgtg caccgccgat gccaagacgg tagccggtga tcacctcgtc gaagatgagg
      961 aggacatcat gcgccgcggt aatttcacga actgctttca ggtatccgtc atcgggaata
     1021 acaggaccga tgtttcccag caccggttca atgatgaacg ctgcgatgtt gtcgtttttc
     1081 gacaacaggg tctcaagtgc tccgggatca ttatagggaa cctgccgcgt atgtgctgca
     1141 aagtcgggaa gaactcctgc cgagtcaggg gtaccgtgtg tcgttgcacc ggaacctgcg
     1201 gcaatcagca ctgcatcgtg cgcaccgtgg aacccgccct cgaccttgac gatgtccgtc
     1261 ttaccggtaa atccgcgggc gagacgcagg gcagccatcg tagcctcggt accggttgag
     1321 acaaaacgga gcatatccat tgacggatga tcattacaga tccgctccgc aagatcaatc
     1381 tccagctcgg taggcgtgcc gtacagccag ccgttgtcaa gctgtgacga gatggcagct
     1441 ttaatcacgg ggtttgcatg tccgaggatc agcggaccgt atccaaggca gcagtcaaca
     1501 agacgggtgt catcgacggt tgtcagcacc gcaccttttg cagatttcac atagaacgga
     1561 tacggtttga ttgcacggac cggactggaa acacctcctg gcagcagagt ctgtgccttg
     1621 tggaacaaag tctcgttttg attcacgcaa gccacctcgc aacatcctgt gcatagtagg
     1681 tgatgatcag atccgcaccc gcacgtttga tacttgtcag ggactccatc acaatccgtt
     1741 tctcatcaat ccatccgttt tgggctgcgg ctttgatcat ggagtattct ccggacacct
     1801 gataggcagc ggtgggaaga ccgagttccc tgacctgggc aagaacatcc agatagaaac
     1861 ttgcaggttt gaccatcaga atgtctgcac cctcttccat gtcaagcaat gactcgcgga
     1921 acgcctcacg gccgtttgcc gggttcatct ggtacgtgga ccggtcgccg aacgagaagc
     1981 cggagtctgc tgcttcacgg aacggaccat acagggcaga tgcaaacttg gtcgagtagg
     2041 aca
//
LOCUS       DALUAJ010000248         2812 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_49466,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000248 DALUAJ010000000
VERSION     DALUAJ010000248.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49466"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="O0X37_05180"
     CDS             complement(<1..899)
                     /locus_tag="O0X37_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62347.1"
                     /translation="MNKYVKAGLALLLMVFVCCSYASAGAVEELNVTYQLGGTYINAE
                     AVASANDGGFFLGMANSSGAILLKTDEDGKEIWRAELPGDDARTVIALEDGGTLSATY
                     TMIVNGTEAEGYTFDGSTWLIRTDSNGNILWQEEIVGIGVGDIAVSNEKIVFAGWFWE
                     SNTGFTQTYLLETGTPANDQIRLGNETASMIPLAMLLEDDGTLVLTGGTTSYLTEESA
                     YAWIAKVKNGVVLSQSVVRTGSTDPLYGEGACGYAITQAQDGGYLIVGSNPPFGVTWA
                     SGLGWAAHVTSDLSNEWVNELPG"
     gene            998..2023
                     /gene="endA"
                     /locus_tag="O0X37_05185"
     CDS             998..2023
                     /gene="endA"
                     /locus_tag="O0X37_05185"
                     /EC_number="4.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015286507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000214 - tRNA-intron endonuclease
                     complex [Evidence IEA];
                     GO_function: GO:0000213 - tRNA-intron endonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-intron lyase"
                     /protein_id="HJK62348.1"
                     /translation="MKAQFDGTQILAPPEAKVFYDQNGYGRVEKTGNIRLAVVEALYL
                     IARGKIQIDGWSFDSLLVESAASPGFLRKFIVYRDIRERGFVITTGPQDFRVFPRGQR
                     PGHGQSKYLLRVLSERDMVDLSVVLSEAQTAATMRKQFLLAVFDDEHELTYYEIRITR
                     PSAKDAVLSSNLEDLPHISATLAGTPAYVVEDGSGITELLKNNWYGTMLDANRLFLAP
                     VEVCWLLESGKLRLTPEMTAEEYEVLAAAYDPEFSDKMTVYRYFRSVGWSPRTGYKYG
                     HHFRVYTEDGKHSEMLVHACTHNTSMPMSVISRSVRLAHSVKKKMLFACMENDEVVFI
                     EFARMKL"
     gene            2046..>2812
                     /locus_tag="O0X37_05190"
     CDS             2046..>2812
                     /locus_tag="O0X37_05190"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HJK62349.1"
                     /translation="MAEQINPWSSTPKMDINRLIADFGIEPFASVKEKITDPPVFIRR
                     DIVAGHRGYNAVADAIAEKKPFHVLTGFMPSGHPHFGHLMVMKEVVWHIQQGGKGFIS
                     MADREAHAVRGLSWDACDQYAREYMECLYALGFSGEIYSQRRNNALKDLAFEAATKVN
                     FSELSAIYGFGPETELAHAVSVSMQVADILYPQLTAGVAPTVVPVGIDQDPHIRLTRD
                     ITNALRMFLVEDRGTHISIRVKNAPKEALDAVAKRFSG"
BASE COUNT          666 a          708 c          696 g          742 t
ORIGIN      
        1 catccgggaa gttcatttac ccactcgttg gagagatcac tcgtaacatg tgctgcccat
       61 cctaaaccac tggcccatgt cacaccaaac ggcgggttag agccgacaat aaggtagcct
      121 ccgtcctgtg cctgtgtgat agcgtatccg catgcacctt cgccgtagag cggatccgtg
      181 ctgccagttc ttacaacgct ctgactaagg acaaccccat ttttgacctt tgcaatccag
      241 gcataggcac tctcttctgt aagataggag gtggttccac cggtgagtac aagtgtaccg
      301 tcatcctcaa ggagcattgc gagaggaatc attgatgcag tttcgttgcc gaggcggatc
      361 tgatcattgg cgggagttcc agtttctaag agatacgttt gggtaaaccc agtgttactc
      421 tcccagaacc atccagcaaa tacaattttt tcatttgaga ccgcaatgtc accgacacca
      481 attccgacta tctcctcctg ccagaggatg tttccgttac tgtcagtgcg aatgagccag
      541 gtactgccgt caaaggtgta tccctctgct tctgtaccat tcactatcat ggtatatgtt
      601 gcagaaagag tccctccgtc ctcaagggca attaccgtcc tggcatcgtc accgggcagt
      661 tcagcacgcc agatctcttt tccatcttca tctgtcttta acagaatcgc accggaactg
      721 tttgccatac caaggaagaa tcctccgtca tttgcagatg caaccgcttc ggcgtttatg
      781 tatgttcctc caagctgata ggttacgttg agctcctcaa ctgctcctgc ggaggcataa
      841 ctgcagcaga caaacaccat cagcagaagg gcgagacctg cttttacata tttgttcatg
      901 tatagtatgt tttacgtttg ggtattagta tgtttgtggc tgtcttccgg gaggaagaca
      961 cccctttaat gattccataa gacaaacgta tactcgcgtg aaggcacaat ttgatggcac
     1021 acagatactg gcaccgccgg aggcaaaggt tttctacgat cagaatggtt acggtcgcgt
     1081 ggaaaaaacc ggaaatatcc ggctcgcggt tgttgaagcc ctgtatctta tagcgcgcgg
     1141 aaagattcag atcgatggct ggtcctttga ttcccttctt gttgaaagtg ctgcatcacc
     1201 cggattcctt cgcaagttca ttgtctatcg tgatatccgt gaacgcggat ttgttatcac
     1261 caccggccct caggatttcc gggttttccc ccgcggtcaa cgtccgggcc atgggcagtc
     1321 taagtatctg ctgcgtgttt tgtcagagcg cgacatggtg gatctctccg ttgttctcag
     1381 tgaggcccag accgctgcca ccatgcgcaa gcagttcctg cttgctgtgt ttgatgatga
     1441 acatgagtta acgtactatg agatccggat tacccgcccc tccgcaaaag atgcggttct
     1501 gtcttctaat ctggaagact taccacacat ttctgcaacg cttgctggta ctcctgctta
     1561 cgttgtagag gatggatctg gcatcacgga acttctcaaa aacaactggt atggtacgat
     1621 gctggatgca aaccggctgt tcctcgcgcc ggttgaggtc tgctggctgc ttgagtcagg
     1681 caagctcaga ctcactccgg agatgactgc cgaagagtat gaagtccttg cggcagcata
     1741 tgatcctgag ttttctgata aaatgacggt ataccggtac ttccgcagtg tcggctggtc
     1801 acctcggacc ggctataagt atggtcatca tttccgggta tacactgaag acgggaaaca
     1861 ttctgagatg ctggttcacg cctgtacaca caacacctct atgccgatga gtgttatttc
     1921 ccggtctgtc cgacttgccc acagtgtcaa gaaaaagatg ctctttgcct gtatggaaaa
     1981 cgacgaagtt gttttcatcg aatttgcccg catgaaacta taacatatca aaattgtgag
     2041 aatttatggc agaacagatt aatccctggt ccagtactcc caaaatggat attaacaggc
     2101 tgattgccga ctttggtatc gaaccgtttg catcggtcaa ggaaaagata acggatccgc
     2161 cggtgtttat ccgccgcgac attgttgcag gtcaccgcgg ctataatgca gttgcggatg
     2221 caattgcaga gaaaaaaccc ttccatgtac ttaccggttt catgccgagt ggtcatcccc
     2281 actttggtca cctcatggtc atgaaagagg ttgtctggca catccagcag ggtggaaaag
     2341 gattcatttc tatggctgac cgtgaagcac atgcagttcg cggcctttcc tgggatgcat
     2401 gtgaccagta tgcccgtgag tatatggaat gtctgtatgc cctcgggttt tccggtgaga
     2461 tatactccca acgtcggaac aatgcattaa aggatcttgc gtttgaggcg gcaacaaagg
     2521 tcaacttctc agagctttcc gcaatctatg gtttcggacc ggagactgag cttgctcatg
     2581 cagtgtcggt ttcaatgcag gtcgcagata ttctctatcc tcagctgact gcaggagttg
     2641 caccgactgt tgtgccggtg ggaatcgatc aggatccgca catccggctt actcgggata
     2701 ttacaaacgc gctccggatg tttctggtgg aagatcgggg aacacatatc agcatccgcg
     2761 taaaaaacgc accaaaagag gcactcgatg cagtggcaaa gcggtttagc gg
//
LOCUS       DALUAJ010000249         2607 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_49574,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000249 DALUAJ010000000
VERSION     DALUAJ010000249.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49574"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..2579
                     /gene="polC"
                     /locus_tag="O0X37_05195"
     CDS             <1..2579
                     /gene="polC"
                     /locus_tag="O0X37_05195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HJK62350.1"
                     /translation="SEEEEPGIHPKDKYMRDMLAGRPPFSYPMRKGGFRLRLGRGRNT
                     GLATCGFNPATLHVLGDYLAVGTQMKVERPGKACGVVPCDTIEGPTVRLRSGEVRRID
                     TLAEANKYVDAEEIEYILDVGEILISYGEFLENNHVLAPPSYCEEWWIQEGGPRHPES
                     EAEAISFVADGAYLHPDYTWFWDDCTEDQIRFLSDKVSETGSVRDGVLYIPVDPQVKA
                     VLEELLVPHTVEEGHYVIRTPLVLIAGLGLSYDLRKNATWNGLPPFTNGLVMAEHLSG
                     IKMRSKAGTRIGGRMGRPGKSAPRKMKPPVHVLFPIGESGGMKRSIDNAAKVCNANLS
                     GGVFSGTAVTTGQVEGMIHVETGERKCPSCGTVTYKSRCPECGTHTEAVYRCPRCNTI
                     SPPNEELCPRCGAPVVCQRDSVLSLRQEYDVALAVTGLSASDIPELKGVRGLISKERV
                     VEPLEKGILRAANDVYVFRDGTVRYDMIDLPLTHFRPAEIAVSVAKLREIGYTKDMHG
                     EELVSPDQVVELNPQDILVSEDCGEYLVRVAKYMDQLLEKLYGLPAFYHAEVPEDLVG
                     QLILGLAPHTSAGVLARLIGFTKAKAGYAHPFYHAAKRRNCDGDEDCVMLLMDGLVNF
                     SRSFLPSTRGGTMDAPLVLTTTLNPKEVDKETLNVDVMDHYPLEVYEACLTYTPPKNL
                     EKIVDHVESRVGTPGQFEGFSFTHDTRDISEGPLDTMYTNPILKGTTDKIKAELELAD
                     RIRAVETDDLAERIINSHLMPDMIGNLRSFSKQSFRCPRCKTSFRRIPISGKCTKCGA
                     VLKATMHKGNVTKYLEIAKYMAEHYQLSEYTNQRIAVTEMNIWSTFGQEEKQQMDLSD
                     FF"
BASE COUNT          612 a          633 c          766 g          596 t
ORIGIN      
        1 cctctgagga ggaagaacct ggcattcatc caaaggataa gtacatgcgg gatatgcttg
       61 caggccgccc gccgttttcg tacccgatgc gcaaaggggg tttccgcctg cggcttggcc
      121 gcgggagaaa caccggcctt gcaacctgcg ggttcaatcc ggcaacactc catgttctgg
      181 gtgattacct tgcggttggt acgcagatga aagtggaacg gccgggaaaa gcgtgcggtg
      241 ttgttccctg tgatacgatc gaagggccga cggtccgtct cagatcggga gaggtccggc
      301 gcatcgacac actcgcggag gcgaacaagt atgttgacgc agaggagatt gagtacatcc
      361 tcgatgtcgg cgaaattctg atctcatacg gagagtttct cgaaaacaat catgtacttg
      421 ctccgccgag ttactgtgag gagtggtgga ttcaggaggg cggcccccgc catccggaga
      481 gcgaggccga ggcgatctct tttgttgcag atggtgcata tctgcatccc gactacacct
      541 ggttctggga cgactgcacc gaggatcaga tccggttcct ctccgacaaa gtttccgaga
      601 ccggttcggt acgggacggt gttttgtata tccctgtgga tccccaggtt aaggctgttc
      661 ttgaagaact gcttgttccg cacacggttg aggaagggca ttatgtcatc cggacaccgc
      721 ttgtactgat tgccggtctt ggtctttcct atgacctgcg gaaaaatgca acatggaatg
      781 gcctccctcc gttcacaaac ggcctggtaa tggcagagca tctttccggc attaagatgc
      841 gctcaaaggc ggggacccgc attggcggca ggatgggccg tccgggaaag tccgctcccc
      901 gcaaaatgaa acccccggta catgtactgt ttccgattgg tgagtccgga ggaatgaaac
      961 gatccattga caacgccgcc aaggtctgta atgcaaatct atcgggcggc gtgttttcag
     1021 gaaccgcggt gaccaccggt caggttgaag gcatgattca cgtagagacc ggtgagcgga
     1081 aatgtccgtc atgcggtaca gtgacctaca aaagccggtg tccggagtgc ggcacacata
     1141 ctgaagctgt gtaccgctgt ccgcggtgca acacgatatc tccgccaaac gaagagttgt
     1201 gtccgcggtg tggtgctccg gtggtctgcc agagggacag tgtcttaagc ctccggcagg
     1261 agtatgacgt tgcacttgca gtaactggtc tttctgcaag tgatatccct gaactgaaag
     1321 gtgtccgcgg tctcatctcg aaagagcgtg tcgttgaacc gctggaaaaa ggaatccttc
     1381 gtgcagcaaa tgatgtgtat gtgtttcggg acggaactgt gcgctatgac atgatcgatc
     1441 tgcccctgac gcatttccgg cctgcggaga ttgcagtttc ggttgcaaaa cttcgggaaa
     1501 ttggctacac aaaagacatg catggtgagg aacttgtctc acccgaccag gtggtggaac
     1561 tgaatccgca ggatattctc gtttctgagg actgcgggga gtatctggta cgggtggcaa
     1621 agtatatgga ccagcttctg gaaaagctgt atggtctccc ggcattttat catgcggaag
     1681 tacccgagga tctggtaggt cagcttattc ttggtcttgc tccgcatacc agtgctggag
     1741 ttcttgcacg gctgatcggt ttcaccaaag cgaaggcggg gtatgctcat ccgttctacc
     1801 atgcggcaaa acggcggaac tgtgacggtg atgaggactg cgtgatgctg ctgatggacg
     1861 gtcttgtaaa cttctcgcgt tccttccttc cgagtacccg tggcggtacg atggatgccc
     1921 cgcttgttct gacaacgaca ctcaacccaa aggaggtgga taaggagacg ctgaacgtgg
     1981 atgtgatgga tcattatcca cttgaggtgt atgaggcgtg tcttacctac accccgccaa
     2041 agaatctgga aaaaatcgtg gatcatgtgg agagcagggt cgggacgccg ggacagtttg
     2101 aaggattttc ctttacgcac gacacccgtg acatctcgga aggtccgctg gacaccatgt
     2161 acacgaatcc tattctgaaa ggaaccacgg acaagatcaa agcagaactg gaacttgcgg
     2221 atcggattcg tgcggtggag acagatgatc ttgcggaacg gatcatcaac agccatctga
     2281 tgccggacat gataggaaac cttcgttcgt tttccaagca gtcattccgc tgtccgcggt
     2341 gtaagacgag tttccgcaga attccaatct cgggaaaatg tacaaagtgc ggtgcggttc
     2401 tcaaggcaac aatgcacaaa ggaaacgtca cgaagtatct ggagattgca aagtacatgg
     2461 ccgaacacta tcagttatcg gagtacacaa accagcgtat tgcggtgacg gagatgaaca
     2521 tctggtcaac attcgggcag gaagaaaagc agcagatgga tctgtcggac ttcttctaat
     2581 cagagtttct cggtacggaa tgttgcg
//
LOCUS       DALUAJ010000250         2922 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_49619,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000250 DALUAJ010000000
VERSION     DALUAJ010000250.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49619"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            78..1337
                     /locus_tag="O0X37_05200"
     CDS             78..1337
                     /locus_tag="O0X37_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62351.1"
                     /translation="MKISHPILIGALILTLLCAGVTAPAAAEVTGQISVINLTMDPEV
                     LMPGDTATVTAVIKNSGTTSVSIGRAMIMGVNEVYSSEDKAYNSVGMLGPGDEMTFTF
                     PITSRGAVGTFYPMLYLDFLDAGLTSLKYTFAVRVDDTQLSVALIDQPDAFGQSRNEK
                     VTIRVGNPRANSLNGIQITVSGDGVSSKQTAYFVGKLEPDQYIDANLTVVVDKPTDIS
                     FDVSYRNGQNLHTNSVQIPVELGERKVRAEMILNNIVVSNKGTYYQITGDVNNAGLED
                     AKSVMVTTAKATPTAPMETYVVGSLEPDGIAEFDVTFVNPESNIIDLVVSYKDADGNP
                     YSTTVPVTLQGAVVSDNNSSSGDDSPLGIIVAVVFILLIAGCAAVAWRRGLLDDIRKN
                     KPCLKFRLSNWLTSIRSTTCRLVMYMP"
     gene            1259..1939
                     /locus_tag="O0X37_05205"
     CDS             1259..1939
                     /locus_tag="O0X37_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HJK62352.1"
                     /translation="MSEVPVVELVDVYKVYHMPAGDVHALNGVTFSVMKGEFVAIMGP
                     SGSGKSTLMNMIGCLDVPTSGDMYIDGRNVKEMTDDELTIHRRDRIGFIFQKFNLIGL
                     LTAYENVEYPMILKYGKRDDTGRPKELLASVGIDEAKMTHTPFELSGGQQQRVAIARA
                     LVNDPALLLCDEPTGNLDSKMSEQIMALLQDLNRKGRTIVMVTHDPHTATYANRTIII
                     RDGEIVNE"
     gene            2034..>2922
                     /locus_tag="O0X37_05210"
     CDS             2034..>2922
                     /locus_tag="O0X37_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HJK62353.1"
                     /translation="MLSMIGIIIGVLAITSLGMMGGAFSAEMTSLLSSSTNSLTIMSV
                     EEKTVDGIPATGLSSKDLRDIEAAIRSATSDYILIPMYQGSKVAKFQNEEFSAQVYGL
                     EPGDIPELVTIQDGKVPRGSEGILIGEKLAEDYDLRIGSRLTMTDKNGATTAVRVVGI
                     LENSGMSLTFSTDSAIVGTTTWYESIANTHNLYSSAMVKTGNLDNLDAISAAIEKRLN
                     GRADRDTDDRVYVMDARQFAEIMQEGLGMVSLFLVAIGGISLLVAAAAIFNVMLMSVN
                     ERIREIGILRSIGTLRNQVL"
BASE COUNT          759 a          696 c          711 g          756 t
ORIGIN      
        1 gaatagaagt acccctcctt ccaaaaagga caaagggaaa gaggttatac aatcacacaa
       61 aaacaggtaa accaataatg aaaatatccc atcccattct gattggagca ctcatcctca
      121 cgctgctgtg tgctggggtc actgctcctg cagctgctga agtcaccgga cagatctcgg
      181 tgatcaacct taccatggac cctgaagtcc tcatgccggg tgacaccgca actgtgaccg
      241 cggttatcaa aaactccgga acaacttccg tctctattgg ccgtgcaatg ataatgggtg
      301 ttaacgaggt ctattcttcc gaagacaaag cctacaacag tgttggtatg ctcggtccgg
      361 gagatgaaat gacctttacg ttcccgatca cctcccgtgg tgcagttggt accttttatc
      421 cgatgcttta tcttgatttc ctcgatgcag gattaacaag cctcaagtac acctttgccg
      481 tccgcgttga tgatacccag ctctcagttg ctctgattga tcagcctgat gcgtttggtc
      541 agagccgaaa tgaaaaggtc actatccgtg tcggcaatcc gcgtgccaac agcttaaacg
      601 gcatccagat tactgttagt ggtgatggcg tctcttccaa gcagaccgcc tacttcgtag
      661 gtaaacttga accggatcag tatattgatg caaaccttac cgttgtcgtg gacaagccaa
      721 cggatatctc cttcgatgta agttaccgca atggccagaa tctgcacaca aactctgttc
      781 agatcccggt tgaactggga gaacgcaaag tccgtgcaga gatgatcctg aataatatcg
      841 tcgtttctaa taaaggaacc tattatcaga tcaccgggga tgtcaacaac gcaggactgg
      901 aggacgcaaa atccgtcatg gttaccacag ccaaagcaac acctactgct ccgatggaga
      961 cctatgttgt cggatccctt gagccggacg gaattgcaga gtttgatgtt acgttcgtca
     1021 atccagaatc caatatcatt gatcttgtcg tatcctacaa agatgcagac ggtaacccgt
     1081 acagtactac agtcccggta acgctccagg gggccgttgt ctctgacaac aacagttcgt
     1141 caggcgatga ctcgcctctt ggcatcattg tagcggtggt gttcatcctt ctgatagccg
     1201 gatgtgcagc tgttgcatgg cggcgcgggc ttcttgatga tataaggaaa aataaaccat
     1261 gtctgaagtt ccggttgtcg aactggttga cgtctataag gtctaccaca tgccggctgg
     1321 tgatgtacat gccttaaacg gtgttacatt ttcagtaatg aagggagagt tcgtggccat
     1381 catgggccca tctggttccg ggaaatccac tctgatgaac atgatcggct gccttgatgt
     1441 tcccacatca ggggatatgt acattgatgg gcgtaatgtc aaggaaatga ctgacgatga
     1501 gcttactatt catcgtcgtg atcgtattgg cttcatcttt cagaaattca acttgatcgg
     1561 tcttctgacc gcctatgaaa atgttgagta cccaatgatc ctcaaatacg gcaaacgtga
     1621 tgataccggc aggccaaagg aactactcgc aagtgtcggc atcgatgaag ccaagatgac
     1681 tcacacgccg tttgagctct ccggcggtca gcagcagcgt gtggccattg cacgtgcttt
     1741 ggtaaacgat cctgcccttc ttctctgtga tgaacccacc ggaaaccttg actcgaagat
     1801 gagtgaacag atcatggcct tattacagga tctaaacaga aaaggccgca caatcgtcat
     1861 ggtcacccat gatccgcaca ccgcaaccta tgcaaaccgg acgattatca tccgtgacgg
     1921 agaaattgtg aatgagtaaa aagaatcaaa acacctcatc tttcagtaag attttctctc
     1981 ccatcatttt tgagctgtct gtcaggaatc ttcgactgaa cttattgcga tccatgctct
     2041 cgatgatagg aatcatcatt ggtgttcttg cgatcacctc tcttggaatg atgggcggtg
     2101 cattttccgc agagatgacc tcgcttctct cctcatctac taactcactg acgatcatgt
     2161 cagtggagga gaaaacggtg gatggtattc ctgcgaccgg tttatcctca aaagatctgc
     2221 gtgacattga agcagcaatt cgttcagcaa cctctgatta tatcctgatt cccatgtatc
     2281 agggttctaa agtggcaaag ttccaaaatg aggagttcag tgcccaggtt tacggccttg
     2341 aacctggtga tattcctgaa ctggtgacaa ttcaggatgg gaaagtcccc cggggttcag
     2401 aaggaatttt aatcggtgaa aagcttgccg aagattatga tctgaggatc ggaagcagac
     2461 ttactatgac cgataagaat ggagcaacca ctgcggtacg ggttgtgggt attctggaaa
     2521 attccggaat gtcactgacg ttctccaccg acagtgccat tgtcggtaca acaacgtggt
     2581 atgaatcgat cgcgaacact cacaatctgt acagttctgc gatggtgaaa accggcaact
     2641 tggataactt ggatgctatc tctgctgcga ttgagaaacg gctgaatgga cgcgctgatc
     2701 gtgatacaga tgaccgggtg tatgttatgg atgcacgaca gtttgcagaa attatgcagg
     2761 agggacttgg catggtttcg ctcttcttag ttgcgatcgg tggtatttcg cttctggttg
     2821 ctgcagccgc catcttcaat gtaatgctga tgagtgtgaa cgaacgtatc cgggagatcg
     2881 gtattcttcg cagtatcgga acactccgga atcaggtgct ac
//
LOCUS       DALUAJ010000251         6040 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_49946,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000251 DALUAJ010000000
VERSION     DALUAJ010000251.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6040)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6040)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6040
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49946"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="O0X37_05215"
     CDS             complement(<1..650)
                     /locus_tag="O0X37_05215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834063.1"
                     /note="unique DNA polymerase that is similar to eukaryotic
                     polymerases; forms a heterodimer of the small and large
                     subunits; possesses polymerization and 3' to 5'
                     exonuclease activities; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II large subunit"
                     /protein_id="HJK62354.1"
                     /translation="MAELATSPRYRAYLDHLISELDRAMEIAQTAKAKGFDPRAEVEI
                     PIASDLAGRVEALLGYKGVAARIRELEERMSREEASLKIGDAFVSREFGETTREEILD
                     HAIRASMALLTEGVVAAPTEGIGKVGIKKNDDGTEYLVIYYAGPIRSAGGTAQALSVL
                     VGDYVRRLLNLDRYKPREEEIERYVEELKQYNSIQSMQYLPKDDDIRLIIRNCPVC"
     gene            complement(650..1132)
                     /locus_tag="O0X37_05220"
     CDS             complement(650..1132)
                     /locus_tag="O0X37_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HJK62355.1"
                     /translation="MTDLNVYFNSLSDASLDVRHAAVEKIAAAGRADPGTVVPVVISE
                     LRSGTLDTRWYLGRSLVKMGPDIIPLILEYAEVEKNMDIQKYFGAVLASFGEIAVPPL
                     VSLFSSSNPMARGMASAALERLEEKAVPALIEAALGDDPQVKLCAELTLTKLNIFEYK
                     "
     gene            complement(1132..2145)
                     /locus_tag="O0X37_05225"
     CDS             complement(1132..2145)
                     /locus_tag="O0X37_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HJK62356.1"
                     /translation="MADINLSGIADSLFNSTGSPFSEIDDPGFLGTQPFLNFGIKMTY
                     GDLIAVLLILIATFIIAKIVSGLVRRMFSGSIEKSNLSFMEKLTRWIIYFFGFLAISG
                     PLGIDFSGLLVAGGIIGVAVGFASQNTLSNVVAGILLMFERPVNLGDNISVKGTEGYV
                     EDIGLMSTTIRTYKGVYVRVPNEAMFTSDITNYVAHVARRFDYKITIRYKDDADKAVK
                     IIKETIEKHPYALKNPAPSVFVDDVGPNGSNLNVRIWSPSGYWWDAKTELLWKIVREL
                     RSAGIVIPFDQNVIWFGHDQTKSTDRAMYGRTASPSNPDEYLQSAAPGRDQQSPENVP
                     QVK"
     gene            complement(2147..2734)
                     /locus_tag="O0X37_05230"
     CDS             complement(2147..2734)
                     /locus_tag="O0X37_05230"
                     /inference="COORDINATES: protein motif:HMM:NF016166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF432 domain-containing protein"
                     /protein_id="HJK62357.1"
                     /translation="MLIHPVEPLYVPDAVTDFLEIRFEEIMIEPNGKTVLFLTFPIEI
                     GVFIDSKGKADVIDVFTFKPPKYSLYGASNRGVITRWHQSKVYYYPPQVKNYEEGVLR
                     LTIENSSGDWVYVSRVILYEKGMYVYYDDQVVSMAAEMTIVNRETADVTGVDRPLHED
                     MTRSLRMYRPRRTTSFANIPGAVIDTTFTMDSGLI"
     gene            complement(3112..4173)
                     /locus_tag="O0X37_05235"
     CDS             complement(3112..4173)
                     /locus_tag="O0X37_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HJK62358.1"
                     /translation="MDAFTLISITLIIIILYIFAKGVVIIQPYQKGLAIRLGTYVGQM
                     NPGFKWVVPFITTVIKLDLRTQVIDVPSQEVITKDNSPTDVDAIIYVRVMDPERAYFE
                     VSNYRQATVALAQTSLRGIIGDMELDEVLYNRDMINRRLRDILDKETDQWGVKIERVE
                     IKEVNPIGAVKQAMTEQTAAERERRAAILRADGEKRAAILKAEGLRQSMILESEGERQ
                     SKILRAEGERQSKILQAQGEAQSLRIVALGSRPLDKRAITVLSLNALEKMADGQATKI
                     IFPFEVSSLIKQGAKFLGAEDLVDNDEALQIELDDSILGDLPDPEEIAKAVESVKPPE
                     SPTADPASADDVPLGGSTR"
     gene            complement(4174..4686)
                     /locus_tag="O0X37_05240"
     CDS             complement(4174..4686)
                     /locus_tag="O0X37_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HJK62359.1"
                     /translation="MDLSFIASSLLLLHYIMLETLLDPAVFPWILIVAGAAFLVIEAA
                     TPGFFMAVPGTAMIVLGLMVLAGVDIFASPVGVGIAVLAAVAAAAVSVLIYRRISPDQ
                     TPVTTGQDTIVGKTGVVTVTVIPDTISGKVDVSGVVWSAKSTGAVLAPGTKIRVTSAS
                     GVHLIVEEVR"
     gene            4830..>6040
                     /gene="pepD"
                     /locus_tag="O0X37_05245"
     CDS             4830..>6040
                     /gene="pepD"
                     /locus_tag="O0X37_05245"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HJK62360.1"
                     /translation="MINGHVLQYFEEITKIPRPSGYEAKMREYLLSFAKSHGFLAKMD
                     AAGNVCIRKPAAAGFEDAAVVVLQAHMDMVCEKDPAKAVNFQTDPIKTVTEGDWIHAC
                     GTTLGADNGVGIALALDVLTAKLPTGPIECVFTVEEETGLTGAEAIGPDFFTGKMLIN
                     LDSEEEGSICTGCAGGVETFAQVSWEPYPAPMDDVYYRIDIDGLAGGHSGTDIHRERA
                     NSIKILAAFLRRIPDADICELSGGNLINAIPRHATAVFGVPAKRTAEVEQIFAQFAEE
                     MRERWSSTDPSISLALLPESPRDEMIPSATKIISALDACPNGVLAMSSTIPDLVQTST
                     NLSSVRMDGRSLIVATFQRSVSQNEKREASEMVAKTFQSCGAEIRTSSEYPGWMPDPS
                     SPLLQTAITVYT"
BASE COUNT         1597 a         1540 c         1507 g         1396 t
ORIGIN      
        1 atacagaccg ggcagttacg gataatgagc ctgatatcat catccttggg aagatactgc
       61 atactctgaa tactgttgta ctgctttagc tcctccacat accgctcaat ctcctcctcc
      121 cgaggcttat aacgatcgag attgaggaga cgccgaacat aatcaccgac cagtaccgac
      181 aatgcctgag cagtaccacc cgcagatcgg atcggccctg catagtagat gacaagatac
      241 tcggtaccgt catcattctt tttgatacca accttcccga taccttccgt cggagcggca
      301 accacaccct cggttaggag tgccatcgat gcacgaatcg catgatccag aatctcttca
      361 cgggtagtct cgccaaactc gcgggacaca aacgcatctc cgatcttcaa agacgcctct
      421 tcacggctca tccgctcctc aagttctcgg atacgagctg caacaccctt gtaacccagc
      481 aatgcctcga cacggcctgc aagatcgctt gcgatcggga tctccacctc tgcacgcgga
      541 tcaaaaccct tcgccttcgc ggtctgtgca atctccatcg cccgatcgag ttcactgatc
      601 aaatgatcca gatatgcacg ataccgggga gatgttgcaa gttctgccat tatttgtact
      661 caaagatgtt gagtttcgtc agcgtcagtt cagcgcagag tttcacctgc ggatcatcac
      721 ccagagcagc ttcaatcagt gccggaactg ctttttcctc aagcctctcg agggctgcgg
      781 atgccatgcc gcgggccatc ggattggagg aggaaaataa tgacacaaga ggcggaactg
      841 caatctcacc aaatgaagca aggactgccc caaagtactt ctgtatatcc atattcttct
      901 ccacctcggc gtactcaaga atgagaggaa taatgtccgg ccccatcttc accagcgatc
      961 gtccgagata ccagcgggta tcaagcgtac cggatcgaag ctcagatatc acaaccggaa
     1021 caaccgttcc gggatcggca cggccagctg cggcaatctt ttcaactgcg gcatgacgaa
     1081 cgtcaagaga cgcatcactg agagaattga aatacacatt aagatctgtc attacttcac
     1141 ctgaggaaca ttttccggag actgctgatc ccgtccagga gctgcggact gaagatattc
     1201 atcgggattt gatggagagg cagtacgacc gtacattgcc ctgtctgttg actttgtttg
     1261 atcatgtcca aaccagataa cattctgatc gaacgggata acaatccccg cagaacgaag
     1321 ttcccggaca attttccaca aaagttcggt ctttgcatcc caccagtacc ccgacggcga
     1381 ccagatacgg acattgagat ttgaaccgtt cggccccaca tcgtccacga acacggacgg
     1441 agccggattt ttcagagcat aaggatgctt ttcgatggtt tccttgatga tcttcaccgc
     1501 tttatcagca tcatctttgt accggatggt aatcttataa tcaaacctgc gggcaacatg
     1561 agcaacgtaa ttcgtaatgt cggaggtgaa cattgcctca tttgggacac gaacgtacac
     1621 tcccttgtag gtacggatcg ttgttgacat caggccgatg tcttcaacat acccttcggt
     1681 tcctttgaca ctgatgttgt cgccaaggtt cactggccgc tcaaacatca gcaagattcc
     1741 agcgaccaca ttggacaagg tgttctgact ggcaaaacct actgcaacac caatgatccc
     1801 tcctgcaacc agaagaccgc tgaagtcaat acccagaggg ccgcttatcg caaggaagcc
     1861 aaagaagtag atgatccacc gcgtcagttt ctccatgaac gaaagattac tcttctcgat
     1921 agatcccgaa aacatccttc ggacaaggcc ggacacaatt tttgcgatga tgaatgtggc
     1981 aataagaatg aggagaacgg caatgagatc accgtacgtc atcttaatcc cgaagttgag
     2041 aaacggctgc gtacccagga atcccggatc gtctatctca ctgaacgggg aacctgtgga
     2101 attaaacaga ctgtccgcaa taccactgag attgatgtca gccatttcag atcagccccg
     2161 agtccatggt aaatgtggtg tcaatgaccg cacctggaat gttggcaaat gatgtggtcc
     2221 tccgcggacg gtacatccgc aacgatcggg tcatatcctc atgcagaggg cgatcaacac
     2281 cggttacatc agcagtctcc cggttgacaa tggtcatctc agcagccatg gaaacaacct
     2341 gatcatcata atagacatac atgcctttct catagaggat aacgcgtgaa acatataccc
     2401 agtctccgga tgagttctcg atggtcagac gaaggacacc ttcctcatag tttttgacct
     2461 gcggaggata gtaatagacc tttgactgat gccagcgggt aataacaccg cggtttgatg
     2521 ctccgtacaa cgagtacttc ggcggcttga acgtgaacac atcaatgaca tcagcctttc
     2581 ctttcgaatc gatgaagaca ccgatttcaa tcgggaacgt caggaaaagc acggtctttc
     2641 catttggctc gatcataatc tcctcaaacc gtatctccag aaagtccgtg accgcatctg
     2701 gaacgtacag cggctcaacc ggatgtatga gcattctccc gttcaaagaa gagatgtttg
     2761 cctcacgaac ccaactacca agggcacggc gatagtgata gagtccctgt gtctgctcaa
     2821 gcgagatgga aagtccggca aagttgacgg cataatcaaa tgtgaatctg ccgtagttaa
     2881 aggaagggat agattcggag gatgcatctc ccgtcggcgg agcagctgac ggaaatttta
     2941 cgggtgccgg attggggcgg ctgctgcggg taattcgtct gagtggtatt gtcgttccct
     3001 ccgtgtctga tgtgatttta ggtaggtggg aaaagtataa tgtagtttct attgaacgaa
     3061 tatacggagc aggcagagga accctctgta aaatgaggcg gaaaaaagag attatctggt
     3121 tgatccgcca agcggaacat catccgcaga ggccggatca gctgtcgggc tctccggagg
     3181 tttgacggac tcaacagctt ttgcgatctc ttcgggatca ggaagatctc caagaatact
     3241 atcatcaagt tcaatctgca gtgcctcatc attatcaaca agatcctcag cgccgaggaa
     3301 ctttgcaccc tgtttgataa gacttgaaac ctcgaacggg aagatgatct ttgttgcctg
     3361 accatccgcc atcttctcca aagcattcag ggaaagaaca gtgattgcac gtttgtccag
     3421 cgggcgggat ccaagtgcca caatccggag actctgtgcc tctccctgcg cctgcaggat
     3481 tttactctgc cgttcaccct ctgcgcggag aatcttactc tgccgttcac cttcagattc
     3541 aagaatcata ctttgacgca gaccttctgc tttgagaatt gccgcacgtt tttctccgtc
     3601 tgcacggaga atcgccgcac gcctttcacg ttctgctgcg gtctgttcag tcatggcctg
     3661 tttgacagcg ccgatcgggt tgacctcttt gatctcgaca cgttcaattt tgacgcccca
     3721 ctgatcagtt tccttatcga ggatgtcccg cagacgacga ttgatcatat cgcggttata
     3781 gagaacttcg tcgagctcca tgtcgccgat gatgccgcgg agacttgtct gcgcaagggc
     3841 tacggttgcc tggcgatagt tggatacctc aaagtaggca cgttcgggat ccataacacg
     3901 gacgtagata atggcatcca catcggtagg agagttgtct ttggtgataa cctcctgtga
     3961 tggaacatcg ataacctggg ttctgagatc gagtttgatg acggttgtga tgaacggtac
     4021 aacccacttg aaccccgggt tcatctgacc gacatatgtt ccgagacgaa tcgcgagacc
     4081 tttctggtac ggctggatga tcaccacacc tttggcaaag atgtagagaa taataattat
     4141 cagtgtgatg gatatgagcg tgaatgcgtc catttagcgg acctcctcaa cgatcaggtg
     4201 aacaccggat gcggaggtca cccgtatttt tgtgccgggc gcaagtactg cccccgtact
     4261 ttttgcgctc cagacgacgc cggaaacatc gaccttgccg gagatggtat ccgggatgac
     4321 cgtcaccgta acaacaccgg tttttccaac gatggtatcc tgaccggtgg taaccggcgt
     4381 ctgatcggga gatattctcc gataaatgag aacactcacc gcggccgcag caacagcggc
     4441 aagtacagcg atgccaacac caacaggaga ggcaaagatg tcaacgccgg caaggaccat
     4501 gagaccgaga acaatcatgg cggttccggg gacagccatg aaaaatcccg gtgttgctgc
     4561 ttcgatgacg aggaaagcag ctccggcgac gatgagaatc cacggaaata ccgcaggatc
     4621 caaaagtgtt tcaagcatga tataatgtag taatagcagg gaagaggcaa taaaggatag
     4681 gtccatgaac gacgaggtcc ctctgccaac aatgatgaga atattctgga ggaaaaacat
     4741 ctgagaatac gaatgcagta tcctccttgg gaaagatgat tattttctgc gtccaatacg
     4801 ttgcagccaa tacacacgca gggacactca tgattaacgg acacgttctg cagtactttg
     4861 aagagattac aaaaatcccc cgaccatccg gatacgaggc aaagatgcgg gaatatctcc
     4921 tttcatttgc aaagagtcac ggatttttgg caaaaatgga tgcggcagga aatgtctgca
     4981 taagaaaacc agccgcagct ggatttgagg atgcggcagt agtcgttttg caggcacaca
     5041 tggacatggt ctgtgaaaag gatccggcaa aagcagtcaa ctttcagaca gatccaatca
     5101 aaaccgtaac cgaaggagac tggatacatg catgcggaac aacgctcggg gcagataacg
     5161 gagtcggtat cgccctcgca cttgacgtgc ttaccgcaaa acttcccaca ggaccgattg
     5221 agtgtgtgtt cacggtggaa gaggagaccg gacttactgg ggcggaagcg ataggacctg
     5281 attttttcac aggaaagatg ctgataaatc ttgacagcga ggaggaggga agtatctgta
     5341 ccgggtgtgc aggtggtgtg gaaacatttg cacaagtttc atgggaaccg tacccggcgc
     5401 cgatggatga tgtatattac cggatagata tcgacgggct tgccggtgga cattccggaa
     5461 ccgacattca ccgggaacgg gcaaactcaa taaaaattct tgccgcattt cttcgaagaa
     5521 taccggacgc agatatctgt gaactttcag ggggaaatct gataaacgcc attccccgtc
     5581 acgcaaccgc agtgtttgga gttcccgcaa aacggaccgc cgaggttgag caaatatttg
     5641 cccagtttgc agaagagatg agggagaggt ggagcagtac agatcccagc atttctcttg
     5701 cccttctgcc ggagtctcca agggatgaga tgattccttc cgcaacgaag attatcagcg
     5761 ctcttgatgc atgtccgaat ggtgttcttg ccatgagcag tacaattccg gatcttgtac
     5821 agacctctac aaacctatcc tcggttcgga tggatggtcg ttccctgatt gtagcaacat
     5881 ttcagcggag tgttagtcaa aatgaaaaac gcgaggcatc agagatggtg gcaaagacgt
     5941 tccaatcctg cggggcggag attcgtacca gcagtgaata tcccggctgg atgcctgatc
     6001 cttcctctcc gctcctgcag acggcaataa cggtgtatac
//
LOCUS       DALUAJ010000252         3939 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_50138,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000252 DALUAJ010000000
VERSION     DALUAJ010000252.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50138"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="O0X37_05250"
     CDS             <1..1634
                     /locus_tag="O0X37_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HJK62361.1"
                     /translation="VSGGLHGVGVSVVNALSTRLTVEVFRDGNIYSMAFSRGGLVQKL
                     ECRPETENEFRERRTRLDNTEAKSPAELTGTRITFQPDGAIFDTTHFDYDILAHRFRE
                     LAYLNKGVELRVADHRTGDSDVFCYEGGLREFVAHLNEGKELLHTDIIYLDKSDTDNK
                     IDVEIALQYNQTYSETLFTFVNSVNTREGGTHLEGFRSALTRAINNSAHANKHLKEDV
                     SVKGEDVREGLTAVISVKIANPQFEGQTKMRLGNSNVKGLVDSMVYQALTEFFEENPK
                     VIAAIAKKAQEAANAREAARRAKELARRKSSLEMSGLPGKLADCSERDPAKSEIYIVE
                     GDSAGGSAKQGRDRKFQAILPLRGKILNVEKAIEHKALKNAEIQTLISAVGAGYGESF
                     DPERARYHHIVIMTDADVDGAHIRILLLTFFYRYMKPLVELGYVYVAQPPLFRIAKGK
                     QERYVYTEEDMRTVVAEFGEKGVVVQRYKGLGEMNAGQLWETTMNPENRILKQVRIED
                     ASYANEIFEKLMGDDVMPRKDFIKRHAGEVKNLDI"
     gene            1624..>3939
                     /gene="gyrA"
                     /locus_tag="O0X37_05255"
     CDS             1624..>3939
                     /gene="gyrA"
                     /locus_tag="O0X37_05255"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012617715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HJK62362.1"
                     /translation="MTSEEQAPLVEKITHRTEPVNIEDEMKNCFIDYAMSVIIGRAIP
                     DVRDGLKPVHRRILYAMFHDENNTSDKAYKKSAKAVAATMGNYHPHGDAAIYDTLVKM
                     AQPFSYRYPLVDGQGNFGSIDGDSAAAYRYTEARLTKAAESLLEDIDKETVDFVPNFD
                     ESSQEPDVLPSRIPNLLVNGTTGIAVGMATSMMPHNLGEVCDLVTAFIDKPDMPIEEM
                     MKILPAPDFPTGGIIMGTDGVRNAYLTGQGKVIVRGVAEVEERKKNYEQIVITEIPYQ
                     VNKAVMIEKIADLVKNKQIEGISDLRDESDKDGIRVIIELKQGVQGNVILNQLYKHTP
                     LESSFGITNLAIVDKKPLILSLAEILRHFIAHRMSVVRRRSQFDLRKAEERKHILDGL
                     LKALDMIDEVIATIRGSPEVAAAQVALVSKFGFSEVQADAILKMQLRRLAALEQQKIL
                     DEKNELQVIIDRLNWILASDEHILSVVKEETAEIRAQYADERRTKIDYSANTDMDVLD
                     FIEDKQVLVMLTSQNYIKRMPLDSCRQQRRGGRGVTGMTTKDEDSVDKVFLASTHDYL
                     LCFTNRGRVYWLRVHEIPEGSRQSKGKAIVNLLNLTDEDVTAVIPLRNFESDKYLFFA
                     TKMGKVGKMSEELFSRPRSGGLIAMTILEGDELVDVMVTDGSCDVVLTTWLGQSLRFS
                     EDEVRPTGRGVQGVKGITLRRGDTVRALTLVEKDHLLTITEGGYGKRTAFDEFRGKGR
                     GTMGVRNIVTNLERGGVVSSLAVGDDEEIVVT"
BASE COUNT          991 a          894 c         1129 g          925 t
ORIGIN      
        1 aggtttccgg cggtctgcac ggggttggtg tctctgtagt aaacgccttg tccacccgcc
       61 tgaccgttga ggtgttccgg gatgggaata tctattcgat ggctttctcc cgcggtggtc
      121 ttgttcagaa actggaatgc cgtcccgaga cggaaaatga gttccgtgag cggcgcacac
      181 gcctggacaa caccgaggca aagagtcctg cagaacttac gggtactcgt atcacctttc
      241 agccggacgg agcaatcttt gatacaaccc actttgacta tgatatcctt gcccaccggt
      301 tccgtgagct tgcttatctg aacaagggtg tggaactgcg tgttgcggat caccgaactg
      361 gcgattcgga tgtgttctgc tatgaaggag gtcttcgtga gtttgttgcc catttgaatg
      421 agggcaaaga gctgctgcat accgacatca tctaccttga caaatcagac accgacaaca
      481 agatcgatgt tgagattgca cttcagtaca atcagaccta cagtgagaca ctgtttacat
      541 ttgtgaacag tgtcaatacc cgggagggag ggacccatct cgaaggtttc cggtcagcac
      601 tcacccgtgc catcaacaac agtgctcatg ccaacaaaca cctaaaagag gatgtctcgg
      661 tcaaaggcga ggatgtccgt gaaggcttaa cagctgtcat tagcgtcaag atcgcaaatc
      721 cccagtttga gggtcagaca aagatgcggc ttggcaacag caatgtcaaa ggtcttgtgg
      781 actctatggt ctatcaggca ctgactgagt tctttgagga aaatcccaaa gtgatcgcgg
      841 cgattgcaaa gaaagcacag gaagctgcaa acgcccgcga agctgcacgc cgtgcaaaag
      901 aacttgcacg gagaaaaagt tcccttgaga tgtcgggtct tcccggtaaa cttgctgact
      961 gttccgagcg tgatcctgca aagagtgaga tctacatcgt ggaaggagat tcggcaggag
     1021 gttccgcaaa gcagggacgt gaccggaagt tccaggccat tcttcccctt cgtggaaaaa
     1081 tcctgaacgt ggaaaaagcc atcgaacaca aggcgctcaa aaatgcggag attcaaactc
     1141 tgatatctgc ggttggtgcc ggatatggtg agtcatttga tccggaacgt gcccgttacc
     1201 accatatcgt tatcatgacg gatgcggatg tggacggtgc acacatcagg attctcctgc
     1261 ttaccttctt ctaccggtac atgaaaccgc ttgtagaact tggctatgtg tatgttgccc
     1321 agccgccgct gttccggatt gcgaaaggaa aacaggaacg ttatgtctat accgaggagg
     1381 acatgcgtac ggtggtcgct gagttcggtg agaaaggtgt tgttgtacag cggtacaaag
     1441 gtcttggtga aatgaacgca ggccagcttt gggaaaccac gatgaacccg gagaatcgta
     1501 tcttaaaaca ggtccgaatc gaggatgcaa gttatgcaaa tgagatcttt gaaaaactca
     1561 tgggtgatga tgtgatgccg agaaaggatt tcattaagcg tcatgcaggg gaggtgaaga
     1621 accttgacat ctgaggagca ggcaccgctc gtcgaaaaga ttacgcaccg taccgaaccg
     1681 gtgaacatcg aagatgagat gaagaactgc tttatcgact atgcaatgtc ggtaattatc
     1741 ggtcgtgcaa ttcctgatgt gcgtgatggt ctgaagcctg ttcaccgccg tattctgtat
     1801 gcgatgttcc atgatgagaa caacacgagc gacaaggcct acaagaaatc ggcaaaggca
     1861 gtggctgcca cgatgggtaa ctaccacccg cacggtgatg cggctatcta cgacaccctt
     1921 gttaagatgg cacagccgtt ctcataccgg tatccgctgg tggatggtca gggtaacttt
     1981 ggttcgattg acggcgactc ggcagcagca taccgttaca ccgaggcacg tctgacaaag
     2041 gctgctgagt cactccttga agacatcgat aaagagacgg tggattttgt tccgaacttc
     2101 gatgagtcgt cgcaggaacc cgatgttctt ccaagcagaa ttccaaatct tctggtgaac
     2161 gggactactg gtattgcggt gggtatggca acaagcatga tgccgcacaa cctgggagag
     2221 gtgtgcgatc tggtgacagc gtttatcgat aaaccggata tgcctatcga ggagatgatg
     2281 aaaattctcc cggcccctga ctttccgacg ggaggcatca tcatgggtac cgacggtgtc
     2341 cgcaatgcgt acttaacggg tcaggggaag gtcattgtcc gcggtgtagc cgaggttgaa
     2401 gaacggaaga agaactatga gcagattgtg attacggaga ttccgtatca ggtaaacaag
     2461 gcagtgatga tcgaaaagat tgccgacctt gtgaagaaca agcagattga gggtatcagt
     2521 gatctgcggg atgagtctga taaggatggt atccgtgtga tcattgagct gaaacagggt
     2581 gtgcagggta atgtgatctt aaaccagctt tacaaacaca caccgcttga gagttcgttt
     2641 ggtatcacga atcttgcgat tgttgataaa aaaccgctga ttctttctct tgcagagatt
     2701 ctccggcact ttatcgctca ccgcatgtcg gtggtccgca gacgttcgca gtttgatctg
     2761 cgtaaagccg aggagcgtaa acacattctc gacggtcttt tgaaggcact ggatatgatc
     2821 gatgaggtca ttgcaactat ccgtggttct cccgaggttg cggcagccca ggttgccctt
     2881 gtcagtaaat tcgggttctc ggaggttcag gcggacgcaa tcctgaagat gcagctgaga
     2941 cggctcgccg ctctggaaca gcagaaaatt cttgatgaaa agaatgagct tcaggtcatc
     3001 atcgacagac tgaactggat cttagcttcc gatgaacaca ttctgtcagt ggtgaaggag
     3061 gagacggccg aaatccgcgc acagtatgcg gacgaacgcc ggacaaagat cgattattct
     3121 gccaacacgg acatggatgt tcttgatttc attgaggaca aacaggttct cgtgatgctg
     3181 acctcgcaga actacatcaa gcggatgccg cttgactcct gccgtcagca gcgccgcgga
     3241 ggccgcggtg tgaccggcat gacaacaaag gatgaagact cggtggataa ggtgttcctt
     3301 gcaagtaccc atgattatct gctgtgtttc acgaaccgcg gtcgtgtcta ctggctgcgg
     3361 gtacatgaga ttcctgaagg gtcacgccag agtaagggca aagcaattgt gaatctgctg
     3421 aacttaacgg atgaggacgt taccgcagtg attcctctgc ggaactttga gtccgacaag
     3481 tacctgttct ttgcaacaaa gatgggtaag gtcggcaaga tgtctgaaga actgttctcg
     3541 cgcccgcggt caggtggtct gattgcgatg accattctgg agggcgatga gctggtggat
     3601 gttatggtga cggacggttc gtgcgatgtt gtcttgacaa cctggcttgg tcaaagtttg
     3661 cggttctcgg aggatgaggt acgaccgacc ggacgcggtg tacagggcgt gaaaggtatt
     3721 actctgcgcc gcggagatac cgtgcgggcc ctgacgcttg ttgagaagga tcatctcctg
     3781 acaatcacgg aaggcggtta tggtaaacgg acggcgtttg atgagttccg cggaaaaggc
     3841 cgcggtacga tgggtgtccg aaatattgtg acgaatcttg aacgcggcgg tgttgtctct
     3901 tcacttgccg tcggtgatga tgaggagatt gtggttacc
//
LOCUS       DALUAJ010000253         6141 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_50223,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000253 DALUAJ010000000
VERSION     DALUAJ010000253.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 6141)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6141)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6141
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50223"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="O0X37_05260"
     CDS             complement(<1..317)
                     /locus_tag="O0X37_05260"
                     /inference="COORDINATES: protein motif:HMM:NF020570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1890 family protein"
                     /protein_id="HJK62363.1"
                     /translation="MTDEVLLLLGCPQIPVQSPIVLYVADLLQDLGKVPVVAANPSAK
                     QLIAASDPKKHYVAEYRDVDRTIADIADGKVRYPLIVSFVHNDAGLTYTATVAALAPE
                     SLLV"
     gene            complement(388..1272)
                     /gene="fhcD"
                     /locus_tag="O0X37_05265"
     CDS             complement(388..1272)
                     /gene="fhcD"
                     /locus_tag="O0X37_05265"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013330399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HJK62364.1"
                     /translation="MEFNGVILEDTYAEGFPVWVARVVITAVTKEWAYKAATEATGFA
                     TSTIGCPCEAGIEKFLPPAETPDNRPGFSILICCSKKALKEQVLERVSECVLTAPTTA
                     VFNGKAGSEEIIPIKLHFFGDGYESKVEVGGIQCWSIPIMGGDFIVEEEIGAVKGVAG
                     GNFLIMGDSQMSALIAAQAAVDAIKGVPGTITPFPGGVVSSGSKVGSKKYKFMGASTN
                     EKFCPTIREKVEETEIPENVKAVYEIVIDGVDEASVMAAMKAGVQAACRVPGVVKITA
                     GNYGGNLGPFKFHLKDCL"
     gene            1412..2323
                     /locus_tag="O0X37_05270"
     CDS             1412..2323
                     /locus_tag="O0X37_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62365.1"
                     /translation="MLSTSPERVKNADTASSVVIGFVLLLVLAAVVLGVWAAVGLPEH
                     LKTQDEQTLEQLERSFLQYKLAADILRIDEESAADVGIILPNMQGLTYSVTKDTGGRL
                     SIIADDDVENTTVIPIGKVSAEFTGFSGERSAVAYEAGGVHLKAAGSNPAWITPAMLQ
                     ISPVDPTAVQIEINQISFADDTEISGSGQLPGTLSLKEVTTKVLYWKDNATLRFESPD
                     PEQRKLWAAVFFETKERTAKGTVYRTAHSDSFTLLVQTQERIPLFSIRQTDPLTLRIE
                     TSTNTTLSRVVLREAIYETGPAEIIYA"
     gene            2316..2744
                     /locus_tag="O0X37_05275"
     CDS             2316..2744
                     /locus_tag="O0X37_05275"
                     /inference="COORDINATES: protein motif:HMM:NF019407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin N-terminal domain-containing
                     protein"
                     /protein_id="HJK62366.1"
                     /translation="MHNDDAVTPVVTSVLLLLMVIGCCATIGVIVFHQMNAEETTVPN
                     VRIQTSAESTHYLYHAGGESLFRNDISIYNLDNDITHRTLVEGDKDWTVWKSGEYLYS
                     EQTLSDVYILWRNGEKEYIIYQYGVNRKTGVTPITDVIPD"
     gene            complement(2741..3469)
                     /locus_tag="O0X37_05280"
     CDS             complement(2741..3469)
                     /locus_tag="O0X37_05280"
                     /inference="COORDINATES: protein motif:HMM:NF013003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HJK62367.1"
                     /translation="MPPRRTRFNQDQYALLRRGSELMDFTEWNRWYAKYLADNLFLRS
                     TDAYGAHLAGADLAYWYLEGADLRFAQLQNADLSYSYLKHANLANANLAGTNLWRAFL
                     AGAELSGANPDAAVYDPAGRIKFDPAQESRLRKAAESGDIAAWNAWYRTELSKEDSDV
                     RLYGAYLEEASLRNLDLSGAVLCYAHLEGADLRNVNLSGANLSHVHLEGADLWQADLT
                     NADLRHAELGGANLAGAKKDHALF"
     gene            complement(3541..4707)
                     /locus_tag="O0X37_05285"
     CDS             complement(3541..4707)
                     /locus_tag="O0X37_05285"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HJK62368.1"
                     /translation="MTVPEITVYSTKQCMYCNNVKAWLAQNNIPYHSIDVGEDPAAAR
                     EMIDLTGQRGVPVIVIDGEVIVGFDRPRIEALLNIDKGKDIPADHELIIIGSGAAGLS
                     AALYSGRKGLETLVIGGVRGGMITRTTDIENYPGFYRISGESLMRLFADQAEDAGAVI
                     LDDVVTDLSVRDGTFVVETLSGRVFTAKSVIAASGRTPKLSGVPGEAELFGKGVSICT
                     TCDGPLFKGKRVAVYGGGNTAAEMALEMSNIAAVVYLISRSSLKADLVTIDRLKEKKN
                     VVIMTGVVITGLHGEGVLTAVETALRQDLANTTRLPIEGLFLGLGLTPNSAVFSGVVG
                     MNSVGEILVDENCRTDVPGLFAAGDVTSVKGKQLGIAVGDGIKASLAAYEYLRK"
     gene            complement(4799..5299)
                     /locus_tag="O0X37_05290"
     CDS             complement(4799..5299)
                     /locus_tag="O0X37_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HJK62369.1"
                     /translation="MDTKTCIADAFAGESQANRKYKSFSEAAADEGYDHVAKLFRATS
                     AAEEIHARRLLRVGGYIGTTVANLEAGMAGELHETNEMYPSFIKVAEAEGRQDAIITF
                     EHAMKAEAVHADLYKQALEAVKAGRDYEVKTVYLCPVCGNIEINKTSDRCPICGIPGA
                     SFKIVE"
     gene            5444..6136
                     /gene="radC"
                     /locus_tag="O0X37_05295"
     CDS             5444..6136
                     /gene="radC"
                     /locus_tag="O0X37_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014867392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HJK62370.1"
                     /translation="MSLKNTAEIDRPREKLIANGPQSLTLAELIAAIIGRGTVKNDAL
                     KIGKAVADILMEHTYTTSIQDIAAVNGMGPAKACQVLAALELARRFPPPDRKSAKIRC
                     AEDLLPFVSQYRYDQQENVIAATLSGAHEILNIRVVTRGLVNQSQIHPREVFADAVTD
                     RAAAIILVHNHPSGNLSPSTRDILMTRTIQEAGEILGIRLLDHLIIGPCDGFLSMLEA
                     FGNDTHENQDLM"
BASE COUNT         1589 a         1608 c         1546 g         1398 t
ORIGIN      
        1 accagaagcg actcaggagc aagggccgca acggttgccg tataggtgag tcccgcatca
       61 ttgtgaacaa aggaaacgat cagcggataa cgcaccttcc cgtcggcgat atcagctatg
      121 gttcggtcaa cgtcacggta ctctgccaca taatgcttct tgggatcact tgccgcgatc
      181 agctgttttg ccgatgggtt tgccgcaacc accggaactt ttccgagatc ctgcaggaga
      241 tccgccacat agagaacaat cggagactgg accggtatct gcggacatcc cagcaggagg
      301 agcacttcat cggtcatacg tggagtacac gtcagtgctg aaaagggata aacattggtc
      361 ttgcggatta cacaaaaaag agagtattta gaggcagtcc ttcaggtgga acttgaacgg
      421 accgaggttg ccgccgtagt ttcctgcggt gatcttgaca acacccggaa ctctgcatgc
      481 tgcctggaca ccagccttca tagctgccat gacgcttgcc tcgtcaacac cgtcgataac
      541 gatctcgtag actgccttga cattctccgg gatctcggtc tcttcaacct tctcgcggat
      601 ggtcgggcag aacttctcgt tggtggatgc tcccatgaac ttgtacttct tggaaccaac
      661 cttggaaccg gacgaaacaa cgcctccggg gaacggagtg atggtgcccg gaacaccctt
      721 aattgcatcg actgctgcct gtgctgcaat gagtgcggac atctgggagt cgcccatgat
      781 caggaagtta ccgcctgcaa cacccttgac tgcaccgatc tcttcctcaa cgatgaagtc
      841 gccgcccata attgggatag accagcactg gattccgccg acttcgacct tggactcata
      901 gccgtcaccg aagaagtgga gcttgatcgg gatgatctct tcagaacctg cctttccatt
      961 gaagactgca gtggtcggtg cggtcagaac acactcggat acacgctcaa gaacctgttc
     1021 cttgagtgct ttcttgctgc agcagatcag aatggagaat cccggacggt tgtcaggagt
     1081 ctctgccggc ggcaggaact tctcaatacc tgcttcacac gggcatccga tagtggaggt
     1141 tgcaaatccg gttgcttcgg ttgctgcctt gtaagcccat tccttcgtta ctgcagtgat
     1201 tacgacacgg gcaacccaca ccgggaagcc ttctgcatat gtgtcttcga gaataacacc
     1261 gttgaattcc atatgttcac cagtttttag tgatagtata caaatgggac atcaaagata
     1321 aagagcattt tctttccaca tggtatcacc atcgacagat cctgaggatc gattcaaata
     1381 atgtcaggga atatagtatc ctgttgacac gatgctatca acctctccgg agcgggtgaa
     1441 aaacgcggat actgcatcat cggttgtgat agggtttgtc ctgctgctgg ttcttgcagc
     1501 cgttgttctt ggagtctggg cagcagtagg gcttcctgaa cacctgaaaa cacaggatga
     1561 acagacgtta gagcagctgg aaagatcatt tctccagtac aagcttgcag ccgatatcct
     1621 cagaatagac gaagagtccg cagcagatgt cgggatcatt ttgccaaaca tgcagggact
     1681 cacatactcc gtcacgaaag ataccggggg aagactcagt atcatcgccg atgatgatgt
     1741 tgaaaacact actgtgattc cgatcggaaa agtttcagca gagttcaccg gtttctccgg
     1801 agaacgatcg gctgttgcct atgaggcagg aggcgtacat cttaaagccg cgggcagtaa
     1861 cccggcatgg ataactccgg caatgctgca gatatcacca gtcgatccaa ctgcggtaca
     1921 aattgagatc aatcagatct ctttcgcgga tgatacagag atcagcggca gcggtcaact
     1981 gccggggaca ctaagcctga aagaggtcac aacaaaagtc ctgtactgga aggacaatgc
     2041 aacgctccgg ttcgaaagtc ctgatcccga acagcgaaag ctgtgggcag cagtgttctt
     2101 tgaaacaaaa gaacgtaccg caaagggaac tgtgtatcgg acagcacatt cagattcatt
     2161 tacgctcctt gttcagacac aggagagaat acctctgttc agtatcagac aaaccgatcc
     2221 gcttacactc aggattgaga cgagtacaaa cacaacacta tccagagtag tcctccgcga
     2281 ggcaatctat gagacaggac ctgcagagat catttatgca taacgacgat gctgttacac
     2341 ctgtggtgac atcagttctc ctgcttctca tggttatcgg atgctgtgca acgattggcg
     2401 tcatcgtttt tcaccagatg aacgcagagg agaccactgt tccgaatgta aggatccaga
     2461 catcagccga aagtacccac tacctgtacc atgcgggagg agaatcgctg ttccgcaatg
     2521 acatttccat ctataatctt gacaatgaca ttacccatcg gaccctggta gaaggagata
     2581 aggactggac cgtatggaaa agcggagagt atctctacag tgaacagacc ctctcggatg
     2641 tgtacattct ctggcgcaat ggggagaagg aatatatcat ctatcagtac ggcgtaaaca
     2701 gaaagaccgg tgttaccccg atcaccgatg tcatccccga ttaaaacaat gcgtgatcct
     2761 ttttcgcgcc cgcaaggttg gcaccgccaa gctctgcgtg ccggagatct gcgttcgtaa
     2821 gatctgcctg ccagagatcg gcaccctcca gatgcacatg ggataaattc gccccggaaa
     2881 gattcacgtt ccggagatcg gcaccttcca gatgtgcata acacagtact gcgcccgaga
     2941 gatccaggtt gcgcagcgac gcctcctcaa gatatgctcc gtacagtcgc acatcactgt
     3001 cctctttgga gagctccgtc cggtaccacg cattccatgc cgcaatgtct ccggactccg
     3061 cagctttacg gagacgggat tcctgtgccg gatcaaactt gattctgccc gcaggatcat
     3121 acactgcagc gtcaggattt gcaccggaga gttcagcacc tgcaaggaat gcccgccaga
     3181 gatttgttcc ggcaagattt gcatttgcca gattggcatg tttcagatag gagtaggaga
     3241 gatcggcatt ctgcagctgg gcaaaccgaa gatcggcgcc ttccagatac cagtacgcaa
     3301 gatccgctcc tgcaagatgt gcaccatagg cgtcagtact gcggagaaag aggttatctg
     3361 caagatactt tgcataccag cggttccact ccgtgaaatc catcaactca gaaccgcggc
     3421 ggagcaacgc atactgatcc tgattaaacc gggtgcgacg tggtggcata gtagggcaga
     3481 cctcgttttg tactgttatg tgcgggtact gtataagaac atgtccctgg ttaatgagat
     3541 ctactttctc agatactcat aggcggcaag cgatgctttg ataccgtctc ccactgcaat
     3601 accaagctgt ttgcccttaa cagaggtgac atcccctgcg gcaaacagcc ctgggacatc
     3661 tgttctgcag ttttcatcaa caaggatctc accaacgctg ttcattccga caacacccga
     3721 gaagaccgca gagttcggag taagaccgag tccgaggaag agaccctcta ttggaagacg
     3781 ggttgtgttt gccagatcct gacgcagtgc agtctcgacg gcggtaagta ctccctcacc
     3841 gtgaagaccg gtgattacaa ccccggtcat aatcaccaca ttcttcttct ctttaagacg
     3901 atcaatggtg accaaatccg ccttcagaga actgcgggag atcagataca cgaccgccgc
     3961 gatgttactc atctcaaggg ccatctccgc ggcggtgttt ccgcccccgt acaccgcaac
     4021 ccgttttcct ttgaaaagcg gcccgtcaca ggtagtacag atggaaactc cttttccaaa
     4081 cagctccgcc tcacccggta caccgctgag cttcggagtt ctgccggatg cggcaatcac
     4141 ggattttgca gtgaacacac gaccagagag tgtttcaaca acgaacgttc cgtcacgtac
     4201 agataaatcc gtaaccacgt catccaaaat aacagcccca gcatcctctg cctgatctgc
     4261 aaacagacgc atcagagact ctcccgatat ccggtaaaaa ccaggatagt tctcaatatc
     4321 agtagttcgt gtaatcattc cgccacgaac ccccccgata accagtgtct caagaccttt
     4381 tctcccggag tacagtgcgg ccgaaagccc tgcggcacca gaaccgataa tgatcagttc
     4441 gtgatcagca gggatatctt tgcctttgtc aatgttgagc agtgcttcaa ttctcgggcg
     4501 atcgaatccc acgatcacct caccgtcaat aacgatcacc ggtaccccac gctggccggt
     4561 aagatcaatc atctctcggg cggcagcagg atcctcacct acatcgatcg agtgataggg
     4621 gatattgttc tgcgcaagcc atgccttcac attattacag tacatacact gtttggtgga
     4681 gtataccgta atctcgggaa cagtcatact ccaacatagg gcgcgtgata attaaaatga
     4741 taggatagga gcacggattg gagagatatg tggaaaaagt gaaaaatgga gagttggatt
     4801 actcgacgat cttgaaggat gcgccgggaa tgccgcagat cgggcagcgg tcagaggtct
     4861 tgttgatctc aatgtttccg caaaccgggc agaggtacac ggtcttaacc tcgtagtcac
     4921 ggccagcctt gactgcctca agtgcctgct tgtagagatc tgcgtgaaca gcctctgctt
     4981 tcatggcgtg ctcaaaggta atgattgcat cctgacggcc ttctgcctcg gcaactttga
     5041 tgaaggaggg gtacatctcg ttggtctcgt gcagttcgcc tgccataccg gcctcaaggt
     5101 ttgcaacggt cgttccgatg taaccaccga cacggagaag gcggcgtgca tggatctcct
     5161 cagctgcgga ggttgcgcgg aaaagctttg caacgtggtc gtatccctca tcggctgctg
     5221 cttcggagaa ggatttgtac ttacggtttg cctgtgactc accggcaaaa gcatctgcaa
     5281 tgcatgtctt agtgtccatg caatattatt tgcagaagtt ggggaataaa ggtttggtat
     5341 gggtacctgt ccatgaaaaa attccaaaag ttcttgaaaa aaagtcagca gagatcgacg
     5401 acacattaat ataatttctt tcccgataac tccattgagt ggcatgagtc tgaaaaacac
     5461 agcggagatc gatcggccgc gggagaagct gatcgcaaac gggccgcaaa gcctgaccct
     5521 tgcagagctc atcgcagcga tcatcggacg cgggaccgta aaaaatgatg cactcaagat
     5581 cggtaaagct gtggcggata ttctgatgga acatacctat actacatcca tacaggacat
     5641 agccgctgtt aacggaatgg gtccggcaaa agcctgtcag gttcttgcag ccttagaact
     5701 tgcccggcgt tttcccccgc cggatcgaaa atccgcaaaa atccgatgtg cagaggatct
     5761 tctgccgttc gtcagtcagt accggtatga tcagcaggaa aacgtcatag cggcaacact
     5821 gtcaggggca catgaaattc tcaacatccg ggtagtgaca agaggcctcg tgaaccagag
     5881 tcagattcat ccccgtgaag tgtttgcaga cgcagtaacc gatcgggcag cagcaattat
     5941 cctcgttcac aaccatccgt cgggaaacct ctcgccaagt acacgggaca ttctcatgac
     6001 cagaacgatt caggaagcag gagaaattct cgggatcaga ctgctggatc atctcatcat
     6061 cggaccgtgc gacggattcc tgagtatgct tgaggcgttc ggaaatgaca ctcatgaaaa
     6121 ccaagacctt atgtgattga g
//
LOCUS       DALUAJ010000254         2907 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_50371,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000254 DALUAJ010000000
VERSION     DALUAJ010000254.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50371"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="O0X37_05300"
     CDS             complement(<1..297)
                     /locus_tag="O0X37_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJK62371.1"
                     /translation="MTDNYDASHITVLEGLAPVRERPAMYIGSTDTRGLHHLVYEVVD
                     NSIDEALAGFCKHISITLNQDGSCTVEDDGRGIPVDIMPKQDKSALEVVMTVLHA"
     gene            complement(518..898)
                     /locus_tag="O0X37_05305"
     CDS             complement(518..898)
                     /locus_tag="O0X37_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing protein"
                     /protein_id="HJK62372.1"
                     /translation="MPHKCTQCGREFRDGSVEILRGCPSCGGKKFLYVSDRVRNADVL
                     EEKSIADIAQETKQEVLEVKNQGPASRPTDILERVESVRILSPGKYELNLERLAESQD
                     IIIGVDDGKYMLDLPSMSKKKQKK"
     gene            complement(929..1303)
                     /locus_tag="O0X37_05310"
     CDS             complement(929..1303)
                     /locus_tag="O0X37_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2073 domain-containing protein"
                     /protein_id="HJK62373.1"
                     /translation="MIQGVQIDMLSSDRLSRMTSMEKIRLILDDVRQGYIVVLEKGLT
                     PEEQGKLLETTMMEISPGGFSGIEIETYPSNNASEGGLFSKLFGGNKNPAGRLMVIGP
                     VDQLKMLTREKDRLIAWASAAQ"
     gene            complement(1310..1936)
                     /locus_tag="O0X37_05315"
     CDS             complement(1310..1936)
                     /locus_tag="O0X37_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013328696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Era-like GTP-binding protein"
                     /protein_id="HJK62374.1"
                     /translation="MAVMMRIKGFFSKLFGKKRSRVGIYGPPNAGKTTLANRIVRDVT
                     GEAIGPVSEVPHETRRARRKEGVEINSGGNKLLIDIVDTPGVTTKIDYHEFLEYGMDQ
                     DEAVARAREATEGVAEAMHWLREDIDGVIYVLDSTQDPFMHVNIMMVGIIESRKLPVI
                     IVANKIDLPDAAPQRIKSAFPQHPVVMVSGLEGNNMDDLYAKIAEVFR"
     gene            2347..2832
                     /locus_tag="O0X37_05320"
     CDS             2347..2832
                     /locus_tag="O0X37_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015284505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HJK62375.1"
                     /translation="MISIGSTTAEAAALMCRDDVGSCIITENGIPKGIVTEQDFNCKV
                     MAKNLLPGSVRVEDVMSSPLITVGEEMPVSVAAKKMIEHKVRRLPVTNEDGQVTGIVT
                     VRDLLGVTNEINEIMTELLVINRPDDKSGMCGVCGAMSADLVPIDGVLVCPNCREQER
                     I"
BASE COUNT          813 a          700 c          662 g          732 t
ORIGIN      
        1 ggcatgcaga accgtcatca cgacttcaag agcgctcttg tcctgtttag gcataatgtc
       61 aaccgggatt ccgcgtccat catcctcaac tgtacaggat ccgtcctggt tcagtgtgat
      121 agagatgtgt ttacagaatc cggcaagagc ttcatcaatg gagttgtcca caacctcata
      181 gacaagatgg tgcagaccac gggtatcggt actgccgata tacatggcag ggcgctcacg
      241 caccggagcg agaccctcga gaacggtgat gtgagacgca tcgtaattgt cggtcattaa
      301 tatgtgatct cctttgaaaa gaaccacccg ggcagggtgt ttctacgaaa tagttaactt
      361 atttttcaaa accgcgcaga ataacagggc gcactctgaa aaatatttat tccttattat
      421 attagtgtga atgacatatt tagattgctg gcggataaaa acgaacagga ttcagagggt
      481 ggaaaaatac gttctaaaaa agatccaaaa aggaggatta tttcttctgt tttttctttg
      541 acatcgacgg cagatcaagc atgtactttc cgtcatcgac accgatgata atatcctgcg
      601 attcggcaag ccgttcgaga ttcagttcat acttccccgg actcagaata cgaacacttt
      661 ccacccgctc cagaatgtcc gtcggccgtg atgcaggtcc ctgattctta acctcaagca
      721 cctcctgttt tgtttcctgt gcaatatcgg caatgctctt ttcctcaaga acatctgcat
      781 tacgcacgcg gtcactgacg taaaggaact tttttccgcc gcaactcgga catccgcgaa
      841 ggatctcgac cgaaccatcg cggaactcgc gcccgcactg ggtacattta tgtggcatgg
      901 ctgtatgttt cgatgaacga aatggtgttt actgtgcagc agacgcccag gcaatcagac
      961 ggtccttctc gcgggtaagc atcttcaact gatcgaccgg tccgatcacc atcagtctgc
     1021 cggcaggatt tttatttccg ccgaacagtt ttgagaacag accaccttct gaagcattgt
     1081 tggaagggta tgtctcgatc tcaattcccg aaaatccgcc gggagatatc tccatcatcg
     1141 ttgtttccag aagttttccc tgctcctcag gagtcaggcc cttttcaaga acaacgatat
     1201 aaccctgacg aacgtcatca aggataaggc gaatcttctc catggatgtc atccggctca
     1261 agcgatcgct tgacagcata tcaatctgaa cgccctgaat catttttctt cacctaaata
     1321 cctcggcgat ctttgcatac agatcgtcca tgttgtttcc ttcaagaccg gagaccatca
     1381 caactggatg ctgcgggaat gcactcttaa tccgctgcgg tgcggcgtct ggcagatcga
     1441 tcttgttagc gacaataatg accggcagtt tgcgggattc aatgatacca accatcatga
     1501 tgtttacatg catgaacgga tcctgagtac tgtcaagcac gtagatgaca ccgtcaatgt
     1561 cttcacggag ccagtgcatt gcctcggcga caccttccgt tgcctcacgg gcacgggcaa
     1621 ccgcctcatc ctgatccata ccatactcaa ggaactcatg atagtcgatc tttgtggtaa
     1681 ctcccggagt atccacaata tcaatcagaa gcttgttacc gccgctgttg atctcaacac
     1741 cctcctttct ccgggcacgg cgggtttcat gcgggacctc actcacagga cctatcgctt
     1801 ctcccgtaac atcacggacg atgcgattgg caagtgtggt tttaccggca ttcggcggac
     1861 cataaattcc aacacgcgac cgttttttac caaacagctt tgagaaaaag ccttttatac
     1921 gcatcattac tgccatgaaa acaccttaat tgggatacgt agtagttcgg ttgtaaaggg
     1981 atataaacgt tcatgttcga agtgaattaa gaataggtca aagaaaaaag aaaattctca
     2041 atatcttcta tctatacccg tcacatctat aaaatataat cttaagagag tataaatacc
     2101 ccccgagagg tacaggaaaa atcgcgaagg atgccgtatt ctcataaaat aaagaataaa
     2161 cattccgcgc gttatttttt gaaaagggct caaaagataa tgaatttata ctgggaagga
     2221 gtattactca ataatcacca gaaaaaacag tgaggaggtg aaatacgtct gatgagaata
     2281 cattctgata tcccggtcaa ccggggagat atacggctgt tagaaataat gaggccgaac
     2341 cctactttga tttctatagg atcaaccacc gctgaggctg cggctctcat gtgcagagac
     2401 gatgtcggca gctgcattat taccgaaaac ggaatcccga aagggatcgt aacagagcag
     2461 gactttaact gtaaagttat ggcaaagaat cttctgcccg gatcggtccg tgtagaagat
     2521 gtcatgagtt ctccgttaat taccgtcgga gaagagatgc cggtgtctgt tgcggcaaag
     2581 aaaatgattg agcataaagt gagaagactg ccagttacaa acgaagatgg acaggtgact
     2641 ggtattgtga ctgttcgtga ccttctcggt gtcactaatg agatcaacga gatcatgacc
     2701 gaacttctcg tgatcaaccg tcctgacgat aagagtggga tgtgcggcgt ctgcggagca
     2761 atgtctgcag atctcgtgcc catcgacggc gtcctggtgt gtccaaactg ccgtgaacag
     2821 gaacggatct aaatacagcc agatgatcac tttacatgca aaatatcatg acaaacaatc
     2881 caaaacaaca gaaggggaac ccacgga
//
LOCUS       DALUAJ010000255         2675 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_50650,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000255 DALUAJ010000000
VERSION     DALUAJ010000255.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50650"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..166)
                     /locus_tag="O0X37_05325"
     CDS             complement(<1..166)
                     /locus_tag="O0X37_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HJK62376.1"
                     /translation="MTSVIHTHHLNLSYGSKQALFDVDFPFEEKKVTALIGPSGCGKS
                     TLLRCFNRMND"
     gene            complement(170..2086)
                     /gene="pstA"
                     /locus_tag="O0X37_05330"
     CDS             complement(170..2086)
                     /gene="pstA"
                     /locus_tag="O0X37_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HJK62377.1"
                     /translation="MTEPTHTAAHTRLLREKIIKSIWLLTALISAAAVVFILGYLIYT
                     ALPAFMEVGIPGFLFNTEWNPTGSDPSYGVAALIVDTLLVTIGALIFAVPLGIASAVY
                     LSYLAPSRIRDTVKPIIELLAGIPSVVYGFFGMVVLCSFLRTTLDIPSGFSWLAASII
                     LGIMALPTIVSVSEDALFAVPKDYKEASLGLGTTYWHTISRVLLPAAASGISAAVVLG
                     MGRAIGETMAVMMVAGNAAIIPDPIWNVLSPVRTLTATLGIEMAEVATGSMHYYALFG
                     VAVILLIIALLVNIASSWIIKRTQTPRSSDSRLSAQSRKWLKYILAAVLCVLFFCFLV
                     AALGVFLAVIVAVLLAVWYFGRAKISSSLAERCSFGVIYLCTGVVILTLAIILWDIFS
                     NGIPAISWEFLTASPSDLGRSGGIYPAIIGTLQLVGGAILVALPIGIGAAIYLIEYSR
                     ENLLTRALRTGNDLLNGTPSIVFGLFGFAFFVIYLNWGICMLAGQICLALMILPTIVR
                     TTEEALKSVPNSLREASLGLGATKWQTIKKVVLPSAAPGILTGAILSIGRAAGETAPI
                     MFTAVVFTKRFVTMDVFDPVMALPFHLYVLSTSVPGATAQQYGTAVVLIVLVMLIYLA
                     AILLRRHFQQKLLR"
     gene            complement(2116..>2675)
                     /locus_tag="O0X37_05335"
     CDS             complement(2116..>2675)
                     /locus_tag="O0X37_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011833490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HJK62378.1"
                     /translation="DLKESEKEGHDFKEYVIGRDGIALIVHPTNTVTELTLSQVKDIY
                     QGTITNWKEVGGSDSEIVLVGRDSASGTREFFTEFVLNDEDAAKTMQELNSNGAVQQS
                     VAQTPGAIGYVSLEYVDDTVKALTIDGVTPSVATVLDGTYTINRPLLMITDGEPTGLA
                     KSYLDFILSAEGQQILSDNGFVPVA"
BASE COUNT          799 a          648 c          676 g          552 t
ORIGIN      
        1 gatcattcat ccggttgaaa cagcggagaa gtgttgactt cccgcagccg gaaggaccaa
       61 taagggcggt aacttttttt tcttcaaacg ggaagtccac gtcaaagagt gcctgtttgg
      121 aaccatacga aaggttcaga tgatgagtat gaataacgct tgtcataatt tatctcagga
      181 gtttctgttg gaagtgtctg cggagaagaa tcgccgcaag atagatgagc atcacaagaa
      241 caatcagcac aacagcagta ccatattgct gtgcggttgc acccggaaca ctggtcgaaa
      301 gaacatacag atggaacgga agtgccatca ccggatcaaa gacatccatc gtgacgaacc
      361 gtttggtaaa caccaccgca gtaaacataa tcggagctgt ctcaccagcg gctctgccga
      421 tggaaagaat tgcaccggtt aaaatacccg gagcagcgga cggcagaaca acttttttga
      481 tcgtctgcca tttggtcgca ccaagaccaa gcgatgcctc gcgaagcgag tttggaacgg
      541 atttaagggc ttcctccgtg gttctgacaa tcgtcggcag aatcatcaaa gcaaggcaga
      601 tctgaccggc gagcatacag ataccccagt taagatagat cacaaagaag gcaaacccaa
      661 acagaccaaa cacaatcgac ggggttccgt ttaagagatc atttccggtc cgcagtgcac
      721 gggtcagcag attttcccta gagtactcaa tcaggtaaat cgcagcacca ataccgatcg
      781 gaagtgcaac aagaattgca ccgcccacca gctgaagcgt accgataatt gccggataaa
      841 tccctcccga acggccaaga tcactgggac tcgccgtcag gaactcccag cttattgcag
      901 gaataccatt ggagaagata tcccagagaa taattgcaag ggtcaggata acaacaccgg
      961 tacagagata aatgacacca aacgaacatc tttcggcaag actgctggaa atttttgcac
     1021 gcccgaagta ccacaccgca agcagtactg caacgatcac cgcaaggaaa acgccaaggg
     1081 ctgcaacaag gaaacagaag aacaacacgc acaaaacagc cgcgaggatg tacttcagcc
     1141 attttctgct ttgagcagaa agccgagaat ctgaagatcg gggcgtctgg gtacgcttga
     1201 tgatccatga agaggcaatg tttaccagaa gtgcaataat cagcagaata actgccacac
     1261 caaagagtgc atagtagtgc atactgccgg ttgcaacctc agccatctca ataccaaggg
     1321 ttgctgtcag agttcggacc ggtgacagga cgttccagat aggatccgga atgatcgcgg
     1381 catttccggc aaccatcatg acggccattg tttcgccgat tgcacgaccc attccaagaa
     1441 caacagcagc ggagataccg gacgctgcgg ccggaagcag aacacgggag atggtatgcc
     1501 agtatgtggt accaagacca agggaagcct ccttatagtc cttaggaaca gcaaaaagtg
     1561 catcttccga cacagaaacg atcgtcggca gagccataat accaagaatg attgatgcag
     1621 caagccagga aaaaccgctc ggaatatcga gggtcgtccg caggaaactg caaagaacaa
     1681 ccataccaaa gaaaccgtac acgacagacg ggatgccggc aaggagttcg atgatgggct
     1741 tgacggtgtc acgaatcctg gacggggcaa gataggaaag atatactgca ctggcaattc
     1801 ccagcggcac agcaaagatc agtgctccaa ttgtgacaag cagagtatca acaattaagg
     1861 cagctacacc ataagaggga tctgatccgg tcgggttcca ctcggtgttg aacaaaaatc
     1921 ccggaatacc cacttccata aatgcaggaa gagcagtata gatcagatat ccgaggatga
     1981 agacaacggc agcagcagaa atcaatgctg tgagaagcca gatggatttg attatttttt
     2041 cccgcaacag acgggtatga gctgcggtat gtgtgggttc tgtcattgtt tcctcaaaaa
     2101 aagattgtgt gagtcttatg cgaccggaac aaatccgttg tcacttaaga tctgctgacc
     2161 ttctgcagag aggataaagt caagataact ctttgcaagg ccggtcggtt caccgtcggt
     2221 gatcatcaga agcgggcggt tgatggtata ggttccgtca aggaccgtag caactgaagg
     2281 agtaactcca tcgatggtga gtgctttaac ggtgtcgtcc acatactcaa gagagacata
     2341 accgattgca ccaggggtct gggcaacact ctgctgaact gcgccgttgg agttgagttc
     2401 ctgcatcgtc tttgcagcat cttcatcatt taagacaaac tcagtgaaga actcccgagt
     2461 accggaggca ctgtcacgac cgacaagaac aatctcactg tcggagccgc caacctcttt
     2521 ccagttggtg attgtaccct gatagatgtc tttaacctgg gaaagggtca gctcagtaac
     2581 ggtgtttgtc gggtgaacga tcagtgcgat cccgtcacgt ccgatgacat attctttgaa
     2641 gtcgtgtcct tctttctcag actctttcag gtcac
//
LOCUS       DALUAJ010000256         2763 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_50654,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000256 DALUAJ010000000
VERSION     DALUAJ010000256.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50654"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="O0X37_05340"
     CDS             <1..398
                     /locus_tag="O0X37_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011448481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HJK62379.1"
                     /translation="VAIAGVLAMEPQVMVLDEPTAGLDPQGVKELFGFLNDLPTSCGV
                     TVIYSTHQVELVPEMADLVYVMEKGEITGAGTPVEIFLQEDLLARAHLELPALPRLIK
                     SLRDHGVAIDPAYTYRDAEQAFLKAFGKL"
     gene            395..1216
                     /gene="cbiQ"
                     /locus_tag="O0X37_05345"
     CDS             395..1216
                     /gene="cbiQ"
                     /locus_tag="O0X37_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HJK62380.1"
                     /translation="MSLIDELFDIEREAYRTSPIHALDARIKLILCLLGLLVTVLLPY
                     GTAELFAFVVIYLLFWGLYILSGSSLRYYLVRLLLIMPFGLFFIILQPFFPNPYYDVY
                     HIAVTLPFGIHMYWESIIFGLSLFAKFVISLSFIILLSATTTMQAMLEGAARLRIPRL
                     FLVVMGLTIRYLYVFALVFQKIQSAFAARCFNGFDRRLPLRYRLNVIGNAAGSLFVRA
                     LDQGERTYAAMCCRGYSAHSAVFYAAKPLHAAEWVFLLFGAGYLVVFPLLVYWMF"
     gene            complement(1222..1614)
                     /locus_tag="O0X37_05350"
     CDS             complement(1222..1614)
                     /locus_tag="O0X37_05350"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HJK62381.1"
                     /translation="MIDAEMSTADLVRLLYNADKSVRNSAAIELGNRGAEGFAAALPL
                     LSDESWVVRYRACEIIGMTKQQEGFSVLLKTLSDPRDHVRYMAVKGLGIFGDPRALPE
                     VTRMLDDENPFVQRIAGKVAAELSELSK"
     gene            1791..2405
                     /locus_tag="O0X37_05355"
     CDS             1791..2405
                     /locus_tag="O0X37_05355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HJK62382.1"
                     /translation="MIPLMLDLSAKRILIFGAGAVGVRKARHFSGCRMTVVSRSLSPD
                     VFSLPQLSIKQMDVADLEDDNLVKLIEKHDFVIAALSDAVQNDRIIRLAKSAGKWYNC
                     ATSDDANFLIPSTVSGDEYTIAVSTSGRAPAVPRFIREDLEDRYEGLDNMIRLLASLR
                     EQLKTTVPDQEKRAEILRAVLRDENIRQRCRENRNADDLVAGYL"
     gene            2405..>2763
                     /locus_tag="O0X37_05360"
     CDS             2405..>2763
                     /locus_tag="O0X37_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HJK62383.1"
                     /translation="MQDTELLTPVAVAGVDYTTFDQTRLAEYRFGNESSFLADAGEHF
                     KGVILLQTCNRVEILVHGTGAALSDYLHSLGRSGFTIHEGTDALRHLLALAAGTKSMI
                     IGEDQILGQLKRALLLA"
BASE COUNT          589 a          716 c          699 g          759 t
ORIGIN      
        1 gtgtggccat tgccggcgta cttgctatgg agccgcaggt gatggtactg gatgaaccta
       61 ctgcaggtct tgatccccag ggtgttaaag agctgtttgg ttttctgaat gatctgccaa
      121 cctcctgcgg cgtcacggtt atctactcca cgcatcaggt ggagctggta ccggagatgg
      181 cggatctggt gtatgtgatg gaaaaaggag agatcaccgg tgcaggaacg cctgtagaga
      241 tctttttgca ggaggatctg cttgcccgtg cccatcttga acttcccgcc cttccccgcc
      301 tgatcaaatc cctgcgtgac cacggcgtag caattgatcc cgcgtacacg taccgggatg
      361 cagaacaagc atttctgaaa gcatttggga aactatgagc ctgatagacg agctgtttga
      421 cattgagcgg gaggcgtacc gcaccagccc gatacatgct cttgacgccc gtatcaaact
      481 gattttgtgt ctgcttggac ttctagtgac tgtcctcctt ccctatggaa ccgcggagct
      541 ctttgcgttc gttgttatct acctgctgtt ttggggattg tatatcctgt ccggatcgtc
      601 actgcggtat tatctggtca ggttgctgct gattatgccg tttggcctgt tctttatcat
      661 cctccagccg ttctttccca atccctacta tgacgtgtac cacattgctg taaccctgcc
      721 gttcgggata cacatgtact gggagtcgat catctttggt ctgtccctgt ttgcgaagtt
      781 tgtgatctcc ctgtcattta tcattctact ctctgctaca accacaatgc aggcgatgct
      841 ggagggggcg gcacggctgc ggattccgcg gctctttctt gtggtgatgg ggctgaccat
      901 ccggtatctg tatgtctttg cgctggtctt ccagaaaatt cagagtgctt ttgcggcacg
      961 gtgtttcaat ggatttgatc ggcgtctgcc gcttcggtat cgtctgaatg tgatcggaaa
     1021 cgctgcgggg tctctgtttg tcagggcact ggatcaggga gagaggacgt atgcggcgat
     1081 gtgctgccgt ggttactcgg cacactcagc ggtattttat gcggcaaaac ctctgcatgc
     1141 agcagagtgg gtgtttcttc tgttcggagc aggatacctg gttgtgttcc cgctgctggt
     1201 gtactggatg ttttaaaaaa gttatttaga tagttctgaa agctctgctg cgactttacc
     1261 ggcaatcctt tgtacaaagg ggttttcatc atccagcatg cgggtaacct caggcagggc
     1321 acggggatcc ccaaatattc caagcccttt tactgccatg taccggacgt gatcgcgggg
     1381 atcggaaagt gttttcagaa gcaccgaaaa tccctcctgc tgctttgtca ttccgatgat
     1441 ctcacatgca cgatagcgga caacccatga ttcgtctgat aagagcggga gtgctgctgc
     1501 aaatccctct gccccgcggt tgcccagttc tattgctgcg gagttccgta cggatttgtc
     1561 tgcattgtag agcaggcgta ccagatccgc ggtactcatc tctgcatcaa tcatactaca
     1621 acatacgaat gttgaagtat ttcatactat ggatttatcc aggtatcctt tccctgatga
     1681 atccgctgtt tttgacagaa tcaaaatgtt ccaaaaaaga gtcagcagat ttcctttgtg
     1741 agtatgaaat acagcacttt aaagtaacac gcaaacccac ataactacca atgattccgc
     1801 tgatgcttga tctctccgca aagcgtatcc tcatctttgg tgcaggagcg gtcggcgttc
     1861 gcaaagcacg ccatttttcc ggctgccgga tgactgttgt ttcccgaagt ctctccccgg
     1921 atgtgttttc cctcccgcag ctttccatta aacagatgga tgttgcagat ctcgaggacg
     1981 acaatcttgt aaaactcatc gaaaagcatg actttgttat tgctgccctt tctgatgcag
     2041 tacagaacga tcgcataatc cgtcttgcaa aatccgcagg aaaatggtac aactgtgcca
     2101 ccagtgatga tgcaaacttc cttattccat ctaccgtatc tggggacgag tacaccatcg
     2161 ctgtctccac ctccggtcgt gcacccgctg tcccgcggtt tatccgtgaa gatctggagg
     2221 accggtatga gggactggac aatatgatcc gtctgcttgc ttccctgcgt gagcagctca
     2281 aaacgaccgt tcccgatcag gaaaaacgtg ctgaaattct ccgcgccgtt ctccgtgatg
     2341 aaaacatccg gcagagatgc cgtgaaaaca ggaatgcaga cgatcttgtc gcaggatacc
     2401 tctgatgcag gacactgaac ttctcacacc agtcgcggtt gccggggttg actataccac
     2461 ctttgatcag actcggcttg ccgaatatag attcggcaac gaatcttcct tccttgctga
     2521 cgccggggag catttcaaag gcgttatcct tttgcagacc tgcaatcgtg ttgagattct
     2581 tgtccatggt acgggggctg cgctttccga ctatcttcac agtcttggac gaagcggttt
     2641 tactatccac gaaggcaccg atgctctgcg tcaccttctt gcgcttgccg caggaaccaa
     2701 atccatgatc atcggcgagg accagatcct cggtcagctc aaacgtgccc tgctgctcgc
     2761 aaa
//
LOCUS       DALUAJ010000257         4315 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_51660,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000257 DALUAJ010000000
VERSION     DALUAJ010000257.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51660"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="O0X37_05365"
     CDS             complement(<1..442)
                     /locus_tag="O0X37_05365"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HJK62384.1"
                     /translation="MATLAGMSGTDVTAMTAELSALLPLWIGKIYQYDNATFGIRLNG
                     EDKARYLLYVVKGVRAHLVSSLPDAPKNPSGFSMYLRKFIDGGKVLAIEQKTVERVLI
                     ISIGKGPWEFRLIIELFDEGNLILTDEKYVIQNALVQKRFRERDI"
     gene            545..1186
                     /locus_tag="O0X37_05370"
     CDS             545..1186
                     /locus_tag="O0X37_05370"
                     /inference="COORDINATES: protein motif:HMM:NF039857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HJK62385.1"
                     /translation="MQVARVKKEKCSTADCNRCIRFCPVSRKDQPVIYIGRNKKATVN
                     EELCNGCGKCVRICPEKAIEMITIPDPVDETASTDSGIQSSSPSEVETPKQTLPPKKK
                     ETPEEKTARKKSEHGERVIRTLIACVLGMIAGIASYFIAGTPSAETGVQADPFIGILI
                     LLVAIVLQRTIFILLKIDTAKLGKKDWFYQAFMTFAMWYLSWTIILSTSLLAE"
     gene            1191..3020
                     /locus_tag="O0X37_05375"
     CDS             1191..3020
                     /locus_tag="O0X37_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis/translation initiation
                     ATPase RLI"
                     /protein_id="HJK62386.1"
                     /translation="MRIAIVHKDRCHSRKCGQECIAYCPRVRTGDETIQIGENGRAEI
                     SEELCVGCGICIKKCPFEALDIIQLPEELDTPVHRYGPNAFVLYGLPQPVAGKVTGVL
                     GPNGIGKSTAVKILSGQVKPNLGIFEREVSWEEILKYYAGTELLDYLQQVAKKTIRAS
                     IKPQYIDFIPKVFKGKVGDLLKANDERNQLDHLIKELALETILEKDLSTLSGGELQRV
                     ALTVALSKEADFYFLDEVTPFLDIYQRMIAAKLIRELAESHPVILVEHDIAILDMVAE
                     TVHIAYGKPAAFGIITRPKGVRIGVNQYLEGFVAEENVRSRDYPVVFETRGHESDVDR
                     EILMEIPEMTKTFPGFTLKVAGGEVRRGEVLGIVGANGIGKSTFAKLLAGVETPDDGK
                     KFDTVTVSYKPQYVTANTSDTVEFLLRQATRRFDSSYYQHEILEPLGLMPLLQSEVNH
                     LSGGELQRVAIALCLSRDADLYILDEPSAHLDVEQRLVTTKVIKRAAEDKGAGIMVID
                     HDMYTIDMISERLLVFDGVPGANGLATGPFEMKEGMNKFLGLLGITFRRDKTGRPRIN
                     KPGSYLDREQKAAGEYYYYNLDMSALSDAEKEDEDETEDSDSQ"
     gene            complement(3041..3535)
                     /locus_tag="O0X37_05380"
     CDS             complement(3041..3535)
                     /locus_tag="O0X37_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HJK62387.1"
                     /translation="MTAADEYFQKGMQAYLVRNFAESIEWFTKALTENPENVNYYYYR
                     GTSYQELEQYSEAVADYSAALDRFSGCVPIRYNRAEICRKLGHADRAAKDLTYIIVHA
                     DRTKGEGHWLALAYLNRGLARIDCGDLETGLVDFGKAEALAKELGDKILLAQIADELE
                     KSGL"
     gene            complement(3532..>4315)
                     /locus_tag="O0X37_05385"
     CDS             complement(3532..>4315)
                     /locus_tag="O0X37_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HJK62388.1"
                     /translation="IYAAKTGMDPVKSVYRSYLQWLYTQDGGLVQSASTGLLAHHEMY
                     VTRAPAATTLAALHSGAMGSIDMRINTSKAADGMMRTAPAGYIAEHDSARILGERIAA
                     LTHGHPDGYRAAGAFSALISYLIDGTALLDAATTVRDETAKLPWGTETAAKLSNAIDL
                     AEKDIPDADAVPQLGTGKTAERALAVGLFCVLRYEDNFSEAVRAAANHGGDSSVTASV
                     AGQIAGACIGREAIPAELTEKLELSAMINDYGMQIGSLRGCL"
BASE COUNT         1117 a         1149 c          972 g         1077 t
ORIGIN      
        1 caatatcccg ctcacggaac cgcttctgca ccagcgcatt ctggatcaca tacttttcat
       61 cggtaagaat cagatttccc tcatcaaaca gttcaataat gagccggaac tcccatggtc
      121 cctttcctat tgaaatgatc aaaacccgct caactgtctt ctgctcgatc gccagaacct
      181 ttccgccgtc gataaacttc cgcaggtaca ttgaaaatcc ggatggattt ttcggggcgt
      241 ctggaaggga actcaccaga tgcgcacgaa ccccttttac cacatacaac aaataacgag
      301 ccttatcctc accatttaaa cgaattccga atgttgcatt atcatattgg tatattttcc
      361 ctatccaaag cggcagaaga gcagaaagct ccgccgtcat tgcagtcaca tccgtcccgc
      421 tcatacccgc aagtgttgcc attgatgata ccaagaatag ataagacctg agaactaaaa
      481 aaataaagca tctatgccgc agctgtctgc agcggcagac cctcccctcc ggagaatacc
      541 aaatatgcag gtagcacgag tcaaaaaaga aaaatgcagc actgcagact gcaatcgctg
      601 catccggttc tgtcccgtct cacgaaaaga tcagccggtt atctacatcg ggagaaacaa
      661 aaaagcaacg gtcaacgaag agctttgcaa cggatgtggt aaatgtgtcc gtatctgtcc
      721 tgaaaaagca attgagatga tcaccatccc ggatcctgtt gacgaaactg cttcaacaga
      781 ttctggcatc cagtcatcct ccccttcgga agtagaaacc cctaaacaga ctctccctcc
      841 aaagaaaaag gaaactcctg aagagaaaac tgcccgcaaa aaatcagaac atggcgagcg
      901 tgtcatacga acgcttatcg cctgtgttct cggaatgatc gcaggtatcg cctcatattt
      961 cattgcggga accccgagtg ctgaaaccgg tgtccaggca gatcctttca ttggtatcct
     1021 gatcctgttg gttgcaattg ttctccagag aacaatcttt atcctcctca agatcgacac
     1081 cgcaaagctc ggtaaaaagg actggttcta tcaggcattc atgacctttg ccatgtggta
     1141 cctttcttgg accatcattc tgagcacatc actcttagcc gagtaatatc atgaggatcg
     1201 ccatcgttca caaagaccgg tgtcactccc ggaaatgcgg gcaggagtgc attgcctact
     1261 gcccgcgggt acggaccgga gatgaaacta ttcagatcgg tgaaaacggt cgtgccgaga
     1321 tctcggaaga gctctgtgta ggttgtggta tctgcatcaa aaaatgtccg tttgaagccc
     1381 ttgatatcat tcagctccct gaagagctgg acacgccggt tcatcgttac ggtccgaacg
     1441 cctttgtcct ctatggtctc ccccagccag ttgcaggaaa ggtgactggt gttcttggac
     1501 caaacggcat tggaaaatca actgccgtta aaattctctc aggccaggtc aagccgaacc
     1561 ttggtatctt tgaacgtgaa gtctcttggg aagagattct taagtactat gcaggaaccg
     1621 aactgctgga ttatctgcag caggtggcaa agaaaactat ccgcgcctcc attaaaccac
     1681 agtacattga cttcatcccc aaagtgttca agggaaaagt tggtgatctc ctcaaagcaa
     1741 atgatgagag aaaccagctg gatcacctca tcaaagagct tgctcttgag accattctcg
     1801 agaaagatct ctcaaccctt tccggtggag agctccagcg tgttgccctg actgttgccc
     1861 tttccaaaga agcagacttc tacttccttg atgaggtcac ccccttcttg gatatttacc
     1921 agagaatgat tgcggctaaa ctgatacgtg aacttgccga gtcacatcct gtcatcttag
     1981 tggagcacga tattgccatt ctggatatgg ttgcagaaac cgtccatatc gcctatggta
     2041 aaccggcagc ttttggtatc atcacgcggc cgaaaggtgt ccgcatcggt gtaaaccagt
     2101 atctcgaagg ttttgttgca gaggaaaatg ttcgtagccg ggactatccg gtagtctttg
     2161 agactcgcgg tcatgaaagc gacgtggacc gtgagatcct catggaaatt ccggagatga
     2221 ccaaaacctt cccaggcttc accctcaaag tggcaggagg cgaggtgcgg cgcggagaag
     2281 ttcttggtat tgttggtgca aatggtatcg gcaaatccac ttttgccaaa cttcttgcgg
     2341 gtgtcgaaac ccctgatgat ggaaagaagt ttgataccgt aaccgtctcc tacaaaccgc
     2401 agtatgttac cgcaaacact tcggacactg ttgagttcct cctgcgtcag gcaaccagac
     2461 gtttcgactc atcctactac cagcatgaga tcctcgaacc actcggctta atgccgcttt
     2521 tgcagtccga agtcaaccac ctttctggtg gagagcttca gcgtgtagca attgccctgt
     2581 gcctttcacg tgacgcagac ctctacatcc ttgatgagcc aagtgcacat ctggatgtag
     2641 aacagagact tgtcacgaca aaagtgatca aacgtgccgc tgaggacaaa ggtgccggta
     2701 tcatggtgat cgatcacgac atgtacacaa tcgatatgat cagcgagcgg cttcttgtct
     2761 tcgatggtgt acctggtgcg aacggtcttg caaccggtcc ctttgagatg aaagagggaa
     2821 tgaacaaatt ccttggtctc ctgggaatca cgttccgccg tgacaagaca ggacgtccgc
     2881 gtatcaacaa accgggatca tacctcgatc gcgaacaaaa agcagcagga gagtattact
     2941 actataatct tgatatgtcc gctctctccg atgcagaaaa agaggatgag gacgaaacgg
     3001 aagattctga ttcccaataa tcttttttca agaaagttag ttataatccg ctcttctcta
     3061 gttcgtctgc aatctgtgca agaagaattt tgtccccgag ctcttttgca agagcctccg
     3121 cttttccaaa atcaacgagt cctgtctcca gatcgccgca gtcaatacgc gccagacctc
     3181 tgttcagata tgccaaagca agccagtgcc cctcaccttt tgttcggtcg gcatgaacaa
     3241 taatgtatgt cagatccttt gctgcacgat ctgcatgtcc aagctttctg cagatctctg
     3301 cacgattgta gcggattggg acacatccgg aaaatctgtc cagcgccgca gagtagtctg
     3361 caaccgcttc ggaatattgt tccagttcct gataggacgt tccgcgataa tagtagtagt
     3421 tcacattctc cggattttca gtaagtgcct ttgtaaacca ctcaatactt tctgcaaagt
     3481 ttcgcacaag atacgcctgc atccctttct ggaaatactc gtctgccgca gtcatagaca
     3541 cccccgcaaa gatcctattt gcattccgta gtcattgatc attgctgaaa gctcaagttt
     3601 ctctgtaagc tcggcgggaa ttgcctccct cccgatgcaa gctcctgcaa tctgtcctgc
     3661 aactgatgcg gtcacacttg aatcaccacc atgatttgct gcagcccgca ctgcctctga
     3721 aaaattgtcc tcataccgga gtacacagaa cagaccgact gcaagcgccc gctccgccgt
     3781 ttttccggtc ccaagttggg ggacggcatc tgcatccggg atatccttct cagccaaatc
     3841 tattgcgttg gaaagttttg ccgcagtctc tgttccccac ggcagctttg cagtttcatc
     3901 tctcactgtg gttgcagcat ccaaaagcgc agtaccatca atcaggtatg agatcagtgc
     3961 cgaaaatgca cctgctgcac ggtatccatc cggatgtccg tgcgtcagtg cagcaattct
     4021 ttccccgaga atccgggcgg aatcgtgttc tgcaatatac cctgcaggtg ctgtacgcat
     4081 catcccgtca gctgctttgc ttgtgtttat ccgcatatca atactaccca tggcaccaga
     4141 atgcagtgct gcaagtgtcg ttgctgccgg tgcacgggta acatacattt catgatgggc
     4201 aagaagtcct gttgatgcac tctgaaccag tcctccgtcc tgtgtgtaca gccattgcag
     4261 gtaagaccga tacaccgact tcactggatc cattcccgtc tttgccgcat aaatc
//
LOCUS       DALUAJ010000258         9807 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_51794,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000258 DALUAJ010000000
VERSION     DALUAJ010000258.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51794"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="O0X37_05390"
     CDS             complement(<1..960)
                     /locus_tag="O0X37_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HJK62389.1"
                     /translation="MALFSKVNKKIGSGSDIEGADSRKGFYWVEDQKGDNVMWRLPRN
                     VMWNDNVLVREDEYGIFFRDGKALAVFDRPDRYALTTQNIPVLKDIAGTVLGNVQIGE
                     FFWVQKREFRDKFGTSQPLAFRDVDFGVVQLRIFGQFSYKVTEPLQLITEFVGTKGLT
                     KSEEIVEWLKGQIVAVLNDTLGELKAKKQMGVLDMPAYLQEIEQLCLAKLTRETEVYG
                     LKIMKFAGLNINMPEEVQEAINKRGAMSALGVNYIQYQSGKAIEGIGTGAAQGGGEGS
                     GFAMMGAGMGAGVGMGNMMAQGMAGMAAGQPAPFGGQPPASQQP"
     gene            complement(960..1496)
                     /locus_tag="O0X37_05395"
     CDS             complement(960..1496)
                     /locus_tag="O0X37_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62390.1"
                     /translation="MTDLRNKVEDDRGLLKKIQLGIPGFRGYRQKEDLRIADSLLRMQ
                     IADLLQSDVLGPLEQIRERAGNALELDLMNDIATVISAAKTAESRVRHAEQGYSGISP
                     AYRVSDEQLNTLYGFDLSLVDGIKGLGTLCRTALGSADTGDFGLVKADLRNIRTELSE
                     LQAVFEKRIETMAGLGAF"
     gene            complement(1524..1850)
                     /locus_tag="O0X37_05400"
     CDS             complement(1524..1850)
                     /locus_tag="O0X37_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HJK62391.1"
                     /translation="MGYTEDKRFQIGWMSVVLMLGIAILVGYSGLGLLAAAGVFLLGV
                     GLIAVALSFAIGKKEPLITGSGALFAIIGAVLMLIYAGAELLLVIGGALAGIALAAIV
                     YVVAKK"
     gene            complement(1939..2484)
                     /locus_tag="O0X37_05405"
     CDS             complement(1939..2484)
                     /locus_tag="O0X37_05405"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HJK62392.1"
                     /translation="MNSKITGALLALAALTVVVFAAGCIGTADQTAQVNDTVNVYYTL
                     TLEDGNVRETNVGESPLTFVLGSGQMIKGFDEAVVGMKPGETKTVTLTPDQAYGEYSY
                     DTYADIPVSLAESYNGGPISIGSTFLMYDYSTGSIQVVQGEVMIIDNESNMTRVVINP
                     PLAGKTLTFEITLDSIGEKTA"
     gene            complement(2521..3696)
                     /locus_tag="O0X37_05410"
     CDS             complement(2521..3696)
                     /locus_tag="O0X37_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012618616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-specific transcriptional regulator TrmB"
                     /protein_id="HJK62393.1"
                     /translation="MVEHKPLKKSSPDAKTDAAVPGGMVARRGQFLQAMRQMTFEAGH
                     FTTAELAEAASVPRSTAQDWINRLLREGCIVTKEEKHGRSPARYASRSAMPSTTCRRI
                     FTTVDGDDVEIFHECLSSGCAGFCEFHHRKAGGAALSVTRDGMLLLERSKIGMPVSEP
                     DLTNAAVGLVSVRREGDTIVQTIRSVYGGPAYSLSSMMGQAKGVCGVSISSADGLVTG
                     EVRTRALVHVTVGIDDTDRKGCGGATFALSQALMKYLTESGDAIGIRHQVAVLSQGIE
                     EKTAGNSCSFLELAVDPETLAGLAAKISRFVEEESVSDNWGVAISQGQHVAPDILALA
                     SRIRSERVTQEEVLTIAKKHGIATFGGRGVIGAVAAVALRTQPQEVLLDLSYPVMDS"
     gene            complement(3683..4672)
                     /locus_tag="O0X37_05415"
     CDS             complement(3683..4672)
                     /locus_tag="O0X37_05415"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HJK62394.1"
                     /translation="MKVALLGLGSVGRGVAQVLSNPSYGFVITAAADSKSGVVAEQGT
                     SLNMDDVFSRKAATGKCGDAAWTADRIVKEAEYDILVEVTPTNAQTGEPALSNIRAAL
                     QRGKHVVTSNKGPVSIAYPELSRLAAEHNVAFLFEGTVAGAVPIINGLTHGLAGNKVH
                     QLFGILNGTCNYVLTRMEEEGLTYSQALDEARDLGYAEADPTYDVNGTDAAIKLVILA
                     NAVFNMGVTLDDVIRIGIDRLTPDALRMAGTTGHTIRLIASIHPDKRLLEVSPRLIPK
                     DHPLVVEGTLNSVTVETELAGPITFIGRGAGSVETASAVLADLLAVKERYGGA"
     gene            complement(4678..5214)
                     /locus_tag="O0X37_05420"
     CDS             complement(4678..5214)
                     /locus_tag="O0X37_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HJK62395.1"
                     /translation="MGDGLLLGLDTVQISLRLELNDKPGQLLAAIKPVSDSGANIISI
                     MHQRGAEASNGTLIVDIVVSLPQSRLDQLKTALTENGIEIIRIGTEHLTCTKTFILIG
                     HILHTDLTDTVNRIDKANIAEVTELHIIMPHITQPSTAKLTIKAAGAAEMEQAFEILM
                     KIAEEKHLLIVDEMEDEP"
     gene            complement(5385..6602)
                     /locus_tag="O0X37_05425"
     CDS             complement(5385..6602)
                     /locus_tag="O0X37_05425"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HJK62396.1"
                     /translation="MEFKPRLVAMPLVVTLLMLIAPVINPYIVGIVVILLSIILYTIH
                     KTHYLAISVAILALLYGIGFIPVIAFLGPLMMMVWGEYLARVLRDYSPETLAFAIGAV
                     LGIVATMFRCMEIDSLVAIIAVVVLLMLRSILVDREDAGMIGLLGVAMTITLFFDLEF
                     TYDATMLALAVVVCAGFAYFAYRAKTVDASGVFAAVLFGIILITFAGVPWFLVVISFF
                     ILGSFFTKYRYAEKVFLGVEQEKSGRRGYMNAFANALVGVAGAILFGLTGNEIFTALF
                     LGCIATATADTLASEIGVTGGTPYLITTLRPVPAGTNGGVTVLGELACLFGALIICGI
                     AFLLEVAPWYVCLIGIAAGVFGTNMDSLIGALIENKGVIGNSGTNLLATLSGGLFAMV
                     VYWICTTAATLLL"
     gene            complement(6724..7827)
                     /locus_tag="O0X37_05430"
     CDS             complement(6724..7827)
                     /locus_tag="O0X37_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HJK62397.1"
                     /translation="MEDVYIARAGALSAKRNTESKIQALCTAAGLETMVSEGDLTAIT
                     VHFGERGNDAFVNALHVAGVIHRVKEIGAKPFLTDTNTLYLGGRRNAVDHIMTALSHG
                     FCFPVTECPVIIADGLKGTNSRDVEVYGKHFDSVKIAADIVAADSMVVVSHFKGHEVA
                     GFGGALKNLGMGCASSEGKREQHTTRPFLKKDSCITCGACINACPEFCISLGGKEIVI
                     DLEHCIGCLMCMNTCPKHAIDIDWKDDGVVFVERMIEYATGAVANKTGKAFYINFLTN
                     ITPHCDCTPWNDIPFVPDIGILASRDPVALDKACYDLVNNAEGVFGSLLPDHHREGEE
                     KFTRVWPGTQPELQFTYAEEMGLGRAEYRLKEI"
     gene            7987..>9807
                     /locus_tag="O0X37_05435"
     CDS             7987..>9807
                     /locus_tag="O0X37_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HJK62398.1"
                     /translation="MEDTKTFAEFAISEELLRAIEDMGFEEPTPIQTMAIPQILEGHD
                     VTGQAQTGTGKTAAFGIPIIEGLDPNNKAVQALVLSPTRELAIQTAEEFSRLMKYKQG
                     LSVVPIYGGQPIERQLKVLRGSVQVVIGTPGRVIDHLERGTLNLDSVRMFVLDEADQM
                     LDMGFREDIENIFQHTPEDRQTILFSATMPAPILDITRRFQHDPQFVKITRRELTVPQ
                     IEQTYIEVRERDKLEALCRLLDMNNPELALVFCNTKRTVDDLMARLSARGYFVEALHG
                     DMKQMQRDRVMARFRGGSIDVLIATDVAARGIDVDDVDMVFNYDVPQDVEYYVHRIGR
                     TGRAGRTGKSVTFVAPREIYKLRDIQRYAKIKIAKTPLPSLDDVAEMKMQMFLDKVRA
                     VMTAGNLEKYLPMVEQLLETEDDSEITSIEVAAALLKMHLDTGKNGSAESSETSSSTP
                     QAEPGSFENTGAEPGMVRFRITLGKNQQIRPKDIVGAFAGECNIPGSCIGRIDLYGNY
                     SFVEVPLEHAAVVLETMGQKTIRGQELEIQTATPRAEREEEERERSFRGNDRSGDRKS
                     YGSSRRGYTGRSGFRSGDRWSADRRSFDRKSQRTESHGFYG"
BASE COUNT         2638 a         2573 c         2273 g         2323 t
ORIGIN      
        1 aggctgttga gaggctgggg gctgacctcc aaacggcgcc ggctgacctg ctgccatacc
       61 cgccattccc tgtgccatca tattgcccat accaactccc gcacccattc cggctcccat
      121 catcgcaaaa ccggagcctt cacccccacc ctgagcagca ccggttccaa taccctcaat
      181 tgccttacca gactggtact ggatgtagtt cacaccaagt gccgacattg caccgcgttt
      241 gttgattgcc tcctgaacct cctcaggcat gttaatgttc agaccggcaa acttcatgat
      301 cttcagtcca tagacctcgg tttcgcgggt cagtttggcg agacagagct gctcaatctc
      361 ctgcaggtat gcaggcatgt ccagaacacc catctgtttc tttgccttca gttcaccgag
      421 agtgtcgttt aacaccgcaa caatctgtcc cttcagccat tcaacgatct cttcagattt
      481 cgtaagaccc ttcgttccaa cgaactcggt aatcagctgg aggggttcgg ttactttgta
      541 ggagaactgt ccaaaaattc tcagctgtac aacaccgaag tccacatcgc ggaatgcaag
      601 gggctggctc gtcccgaatt tgtcccggaa ctcacgcttc tgtacccaga aaaattcacc
      661 tatctggacg tttcccagaa ctgttcctgc aatatccttt aggacaggaa tgttctgtgt
      721 tgtcagtgca taccggtcgg gacgatcaaa gacggcaagg gcctttccgt cacggaagaa
      781 gataccatac tcatcctcac gaaccagtac gttgtcattc cacatcacat ttcttggaag
      841 gcgccacatc acattgtcgc ctttctgatc ctcaacccag tagaatcctt ttcgggagtc
      901 tgctccctca atgtcggatc cggagcctat ctttttgtta actttggaga ataacgccat
      961 tagaatgcac caagtcctgc cattgtctca atacgtttct caaacaccgc ctgaagttct
     1021 gaaagttctg ttctgatgtt gcggagatcc gcttttacca acccaaaatc accggtatca
     1081 gcagaaccga gtgctgtacg gcacagtgta cctagtccct taattccgtc aacaagagag
     1141 agatcaaatc catacagtgt gttcagctgc tcatcactaa cgcggtatgc cggagagatt
     1201 cctgagtatc cctgctctgc atgacgaacg cgtgactcag cggttttcgc tgcagagata
     1261 actgttgcaa tatcattcat cagatccagt tccagcgcat tcccggcacg ctcacggatc
     1321 tgttcaaggg gaccaagaac gtccgattgc aacagatcag caatctgcat tctaagaagg
     1381 gagtctgcaa tacgcagatc ttctttctgc cgatatccgc ggaatccggg aattcccagc
     1441 tgaatctttt tcaaaagtcc gcgatcatcc tcaactttgt ttcgtaaatc cgtcattttt
     1501 cctcttattt ttgagaaatc aaattatttc tttgctacaa catagacgat tgcagcaagt
     1561 gcaataccgg caagtgctcc gccaatgacc agtaaaagtt ctgctcctgc gtaaatcagc
     1621 atcagaaccg caccgatgat ggcaaacaat gcaccagaac cggtgatcag tggttctttt
     1681 ttgccgattg caaaggacag ggcaaccgcg atgagtccaa caccaagcaa gaacacaccc
     1741 gcggcagcca gcaggccgag gccagaatat ccaaccagaa ttgcgatgcc gagcatcaga
     1801 acaacactca tccatccaat ctggaaccgt ttatcttcag tatatcccat agctattcgt
     1861 ggtacgtatg tacttattcg gagatgtgga catcatgagg caaagaactt tttccagaac
     1921 attctgttca aaaaaagatt atgcggtctt ctcaccgatg gaatcaagcg tgatctcaaa
     1981 ggtcagcgtt tttcctgcga gtggtggatt gatgacaaca cgggtcatat tgctctcatt
     2041 atcgatgatc atgacctctc cctgcactac ctgaattgaa ccagttgagt aatcgtacat
     2101 caggaaggta gaaccaattg agataggacc tccattgtag ctttctgcaa gggaaaccgg
     2161 aatgtcagca tatgtatcat aactgtattc accgtacgcc tgatcaggag tcaaagtcac
     2221 cgtctttgtt tcacctggtt tcataccaac tacagcctca tcaaatccct taatcatctg
     2281 accagaccct aatacaaaag taaggggaga ttcgccaaca tttgtctcac gtacgttccc
     2341 gtcttccagc gtcagcgtat aatacacgtt taccgtatcg ttcacctgtg cagtctgatc
     2401 tgcagttcca atacatcctg cggcaaacac cacaacagta agggctgcaa gagccagcaa
     2461 agccccggta atttttgagt tcatgtaaaa tatattttac actcaattgt ataaaagtgt
     2521 tcagctatcc ataacaggat acgaaaggtc caacagtacc tcctgcggct gggtacgaag
     2581 tgcaacagct gcaacggcac caatcactcc tcgaccacca aatgtcgcaa ttccatgctt
     2641 ttttgcaatg gtcagcactt cctcctgcgt cacccgttca gatcggatac gcgaagcaag
     2701 cgcgagaata tccggtgcga catgctgacc ctgggaaatt gcaacacccc agttgtcaga
     2761 aacgctttcc tcctccacaa accggctaat ctttgccgca agcccagcaa gagtttccgg
     2821 atcaactgca agttcaagga aactgcatga gtttccagca gttttctcct caataccctg
     2881 tgacaaaaca gccacctgat gccgtatccc tattgcatct ccactttcgg taagatactt
     2941 catcagtgcc tgagacaggg caaacgtagc tccgccgcac ccttttcgat cggtatcatc
     3001 aatacccacc gttacatgta cgagagcgcg tgtccgcact tccccggtaa ccagaccatc
     3061 agcagaagaa atggatactc cgcaaacacc ctttgcctgc cccatcatgg atgacaaaga
     3121 ataagcagga ccgccataca cggaacggat cgtctgaacg atcgtatccc cctcccgcct
     3181 gaccgaaaca agacctacag ccgcattggt gagatccggt tcggaaacag gcatcccaat
     3241 tttactgcgt tccaaaagaa gcataccatc ccgtgtaaca gacagggcag caccccctgc
     3301 ttttcggtga tggaactcgc agaaccctgc acatccgctg ctcaggcact catgaaaaat
     3361 ttcaacatca tctccgtcca ccgttgtaaa aatccggcgg caggtggtgg acggcattgc
     3421 acttcgtgat gcgtaccgag cggggcttct cccgtgcttc tcttccttgg taacaatgca
     3481 gccttcccgc agaagccggt tgatccagtc ctgcgcggtt gagcggggga cagaagcagc
     3541 ctccgcaagt tcagcagtgg tgaaatgacc agcttcaaac gtcatctgac gcattgcctg
     3601 cagaaactga ccgcgccgcg caaccattcc tccgggaacg gctgcatctg tctttgcgtc
     3661 tggcgaactc tttttcagag gtttatgctc caccatagcg ctccttgacc gcaagcagat
     3721 ctgcaagaac agcacttgca gtctcaacag atcccgctcc tcttccaatg aacgtaatgg
     3781 gaccggcaag ttcagtctcc accgtcaccg agttcaaagt accctccact accagtggat
     3841 gatccttggg gatcagcctg ggtgatactt caagcagtcg tttatctgga tgaatggagg
     3901 caatcagccg aatggtatga ccggtcgtac ctgccatgcg caatgcatcg ggtgtgagcc
     3961 tgtcaatacc gatccggatc acgtcatcca gcgtaacacc catgttgaat acagcatttg
     4021 ccagaatcac cagtttgatt gcagcatccg ttccgttcac atcgtacgtg gggtcggcct
     4081 cagcataacc gagatcgcgc gcctcatcaa gtgcctggga ataagtaaga ccctcttctt
     4141 ccattcttgt aagaacgtaa ttgcaggtac cgtttaagat gccgaacagc tgatggactt
     4201 tgtttcctgc aagaccgtgt gtgagaccgt tgataatggg aactgcccct gcaacggttc
     4261 cctcaaaaag aaatgccaca ttatgttctg ccgcaagccg ggaaagctcc gggtaagcga
     4321 tagagaccgg ccctttattt gaggttacaa catgtttccc ccgttgtagt gcagcacgga
     4381 tattggacag ggccggctca ccggtctgtg cattggttgg cgtaacctca acaagaatat
     4441 catactcagc ctctttaaca atccggtctg cggtccatgc agcatccccg catttgccgg
     4501 tcgcagcttt gcgggaaaac acatcatcca tattaaggga tgtcccctgc tcagcaacaa
     4561 caccgctttt tgagtcggct gcagcagtta tcacaaatcc gtaggatggg tttgaaagaa
     4621 cctgggcaac acctctgcca actgacccaa ggccaagaag agcaactttc atgggggtca
     4681 tggctcatcc tccatctcat caacaatcaa aagatgtttt tcttcagcaa tcttcatcag
     4741 aatctcaaac gcctgctcca tctcggcagc acccgcagct ttgatcgtaa gttttgctgt
     4801 ggacggctgg gttatgtgcg gcataatgat gtgaagctcg gtcacttcag cgatatttgc
     4861 cttatcgata cggttaacag tgtccgtcag atctgtatgc agaatatgac caattaaaat
     4921 aaacgtcttc gtacaggtca gatgctctgt tccaatacgg atgatctcaa tgccattttc
     4981 agtgagcgcc gtcttcaact gatcgagacg gctttggggg agtgagacta caatgtccac
     5041 gatcagggtt ccgttgctcg cttctgcacc gcgctggtgc ataatagaga taatattcgc
     5101 accgctgtcg gaaaccggct taattgccgc aaggagctga cccggtttgt cattgagttc
     5161 aagtcgtagt gatatctgca ccgtatctaa tcccaataat aatccgtcac ccatccattt
     5221 taatgtgggg tttgtcagcg gagatgaacc acaactccaa aatcagatgc taagtggaca
     5281 actgttttcc cagtgatata agatggttag aagacagtat ggttttggaa aaaccagtat
     5341 cccaaaataa cctgcacgat actggattac accgctcaaa aatatcagag aagaagagta
     5401 gctgctgtcg tacagatcca gtaaacgacc atggcaaaca aaccaccgga aagtgttgca
     5461 agcaggtttg ttcccgaatt accgataaca cctttgtttt caatcagggc accgatcaaa
     5521 ctatccatat tggtaccgaa aactccggca gctataccta tcagacacac ataccagggg
     5581 gcaacttcca gcaggaatgc aatgccacag ataatgagtg caccaaacag acaggcaagc
     5641 tcaccgagaa ccgttacccc tccattcgtc ccggcaggta ctggccgcag ggttgtgatc
     5701 agatacggag tccctcctgt aacaccgatc tcactggcaa gggtatcagc agttgcggtt
     5761 gcaatacatc caagaaagag tgccgtaaat atctcatttc cggtgaggcc gaagagaatt
     5821 gcaccggcaa cgcccacaag cgcattggca aatgcgttca tgtaaccacg gcgaccactt
     5881 ttctcctgct caacgccgag gaagaccttt tccgcatacc ggtatttggt aaagaaggat
     5941 cccagaatga aaaaactgat aaccacaagg aaccaaggca cacctgcaaa cgtaatcaag
     6001 ataatgccaa acagaaccgc tgcaaaaacg ccgcttgcat caactgtttt tgcccggtag
     6061 gcaaagtagg caaaccctgc acagacaaca acggctagag caagcatcgt tgcatcatac
     6121 gtgaactcca gatcaaagaa cagggtgatg gtcattgcca cacctaaaag cccgatcatc
     6181 cctgcatcct cgcgatcgac aaggatgcta cgcaacatga gcagtacaac aacggcaatg
     6241 atcgcaacca ggctatctat ctccatacag cggaacatgg ttgcaacaat accaagaacc
     6301 gcacctatgg caaacgccag tgtttccggc gagtagtccc gcagcacgcg ggcaagatat
     6361 tctccccaca ccatcatcat tagaggacca aggaaggcaa tgacagggat gaaaccaata
     6421 ccatacagca gggcaagaat tgcaacagaa attgcaagat aatgggtttt gtgaatggta
     6481 tagagaataa tggacagaag gatgaccact attccaacga tataggggtt gataaccggc
     6541 gctatgagca tcagcagtgt taccacaagc ggcattgcga ccaagcgcgg tttgaactcc
     6601 attgagtgta catgttagag tacactctgc aataaggatg ttcgtcgaat agactttaca
     6661 tccaccaaga aggtttacaa aaatcagaag ataccaaaaa taatcggatg ttaaagagag
     6721 accttaaatc tcttttaacc ggtattccgc tcggccaagc cccatctctt ccgcatacgt
     6781 gaactggagc tctggctgtg ttcctggcca tacacgtgtg aacttctcct caccttctcg
     6841 atggtgatca ggcagaagac ttccaaaaac accttcagca ttgttgacca gatcataaca
     6901 ggccttatcc agtgcaaccg gatcgcggga tgcaagaatg ccgatatccg gcacgaacgg
     6961 aatatcattc catggtgtac agtcacagtg cggagtaatg tttgtcagga agttaatgta
     7021 gaatgccttt ccagtcttat ttgcaacagc tcctgtagcg tactcaatca tcctttccac
     7081 aaacacgacc ccatcatcct tccagtcaat gtcaattgca tgtttcggac aggtgttcat
     7141 gcacatcaag cagccgatac agtgttcaag atcgataaca atctcttttc caccgagact
     7201 aatacaaaac tcagggcatg cattaataca agccccacag gtgatacagg agtctttctt
     7261 gaggaacgga cgagttgtat gctgttcacg tttcccctca cttgaagcac aacccattcc
     7321 aagatttttg agagctccac caaatcctgc aacctcatga cctttaaagt gggaaaccac
     7381 aaccatgctg tcagcggcaa caatatctgc tgcaattttc acagaatcga agtgttttcc
     7441 atacacctca acatctctgg aatttgttcc tttgagaccg tcggcaataa ttaccggaca
     7501 ctctgtcact ggaaaacaga atccatgaga aagtgcagtc ataatgtgat ctaccgcatt
     7561 tctgcgtccc cccagataca gagtgtttgt gtcggtcaga aacggttttg ctccgatctc
     7621 ttttaccctg tgaataacac ctgcaacatg cagggcatta acgaatgcat cgtttccacg
     7681 ctcaccaaag tgcactgtga ttgcggtaag atctccctct gaaaccatag tctcaagacc
     7741 tgctgcggta cagagagcct gaattttact ttcggtgttg cgcttagcag aaagtgcgcc
     7801 tgccctggca atgtagacat cctccatata ataatctcat tcgtcaggat tgctatttct
     7861 aatattgggt tttgaaaaat cctgacaata ggttcgcaaa agaggagacc aacacctgaa
     7921 tctttgagaa ggattattcc aatttctggg ttactatata tcctcagccc tcctatatta
     7981 ttgagaatgg aggatacgaa gacattcgca gagttcgcaa tttctgaaga gcttcttcgg
     8041 gcaattgagg acatgggatt tgaggagccg acacctatcc agacaatggc gattccgcag
     8101 atccttgagg ggcacgatgt gacagggcag gctcagacgg gtacggggaa aacggcggcg
     8161 ttcgggattc caattatcga ggggcttgat ccaaataaca aagcggtgca ggcgttagtg
     8221 ctctcaccaa ccagggaact tgcaatacag acggccgagg agttttcccg gctgatgaaa
     8281 tacaaacagg gactgagtgt ggttcccatc tatggcggac aaccgatcga gcgccagctg
     8341 aaggtgcttc gcggttctgt tcaggtggta atcggaacac ccggaagggt tattgatcac
     8401 cttgaacgcg gcacactgaa tcttgattcg gtccggatgt tcgtgcttga tgaagcagat
     8461 cagatgctgg acatgggttt ccgcgaggat atcgagaata ttttccagca cactccagaa
     8521 gatcgtcaga cgattctgtt ttcagcaaca atgcctgcgc cgattctgga cattacgcgc
     8581 agattccagc atgatccaca gtttgtgaag attacacgcc gcgaactgac ggtgccgcag
     8641 attgagcaga cctacattga ggtccgcgaa cgtgataagc ttgaggcact ttgccggttg
     8701 ctggatatga acaacccgga gctggcactg gtcttctgta atacaaaacg aacggttgac
     8761 gatctgatgg cacgcctgtc tgcgcgcgga tatttcgtgg aagcactgca tggtgatatg
     8821 aaacagatgc agcgcgatcg ggtgatggcg cgtttccgcg gtgggtctat tgatgtgttg
     8881 attgcgacgg acgttgcagc ccgcggcatc gatgttgatg acgttgatat ggtgttcaac
     8941 tacgacgttc cgcaggatgt tgagtactat gtgcacagaa tcggcagaac aggacgtgca
     9001 ggcagaaccg gaaaatcggt gacatttgtt gcaccgcgcg agatctacaa actccgcgat
     9061 attcagcggt atgcaaagat caagattgca aaaacgcccc tgccgtcact ggatgatgtt
     9121 gcagagatga agatgcagat gttccttgac aaagtccgtg cagtgatgac tgcaggaaac
     9181 cttgagaagt atctgccaat ggttgagcag ctgcttgaga ctgaggacga ttctgagatc
     9241 accagcatcg aggttgcagc agcacttctg aagatgcatc ttgataccgg caaaaacggt
     9301 tcggcagaga gtagtgagac ctcttccagt acgccgcagg cagaacctgg ttcctttgaa
     9361 aatacaggtg ctgagccagg catggtcagg ttcagaatta cgcttggaaa aaaccagcag
     9421 atccgcccaa aggacattgt aggtgcattt gcgggggagt gcaatatccc tggaagctgt
     9481 atcggacgaa tcgatctgta cggcaattac tcgtttgtcg aggttcccct ggaacatgca
     9541 gcagttgtcc ttgagaccat gggtcagaaa acaatccgcg gtcaggagct ggagattcag
     9601 actgcaacac caagagctga acgtgaagaa gaggaacgcg aacgttcatt ccggggaaac
     9661 gatcggtccg gggatcgtaa atcatatggc agcagccgca gaggatacac cggccgtagt
     9721 ggattccggt caggggatcg ctggagtgca gaccgcagga gttttgacag gaaaagccag
     9781 cggaccgaga gccacggatt ctacggc
//
LOCUS       DALUAJ010000259         3459 bp    DNA     linear   ENV 11-MAY-2023
DEFINITION  MAG TPA_asm: Methanocorpusculum sp. isolate HAC023.59 k141_51999,
            whole genome shotgun sequence.
ACCESSION   DALUAJ010000259 DALUAJ010000000
VERSION     DALUAJ010000259.1
DBLINK      BioProject: PRJNA907943
            BioSample: SAMN32062330
            Sequence Read Archive: ERR2193609
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Methanocorpusculum sp. (feces metagenome)
  ORGANISM  Methanocorpusculum sp.
            Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
            Methanomicrobiales; Methanocorpusculaceae; Methanocorpusculum.
REFERENCE   1  (bases 1 to 3459)
  AUTHORS   Volmer,J.G., Soo,R.M., Evans,P.N., Hoedt,E.C., Astorga Alsina,A.L.,
            Woodcroft,B.J., Tyson,G.W., Hugenholtz,P. and Morrison,M.
  TITLE     Isolation and characterisation of novel Methanocorpusculum species
            indicates the genus is ancestrally host-associated
  JOURNAL   BMC Biol 21 (1), 59 (2023)
   PUBMED   36949471
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3459)
  AUTHORS   Volmer,J.G.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-DEC-2022) Diamantina Institute, University of
            Queensland, Kent Street, Brisbane, Queensland 4102, Australia
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 2020
            Assembly Method        :: MegaHit v. 1.1.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 1.44621x
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 12/29/2022 18:53:43
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.4
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,089
            CDSs (total)                      :: 1,062
            Genes (coding)                    :: 1,059
            CDSs (with protein)               :: 1,059
            Genes (RNA)                       :: 27
            tRNAs                             :: 27
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 3
            CDSs (without protein)            :: 3
            Pseudo Genes (ambiguous residues) :: 0 of 3
            Pseudo Genes (frameshifted)       :: 0 of 3
            Pseudo Genes (incomplete)         :: 3 of 3
            Pseudo Genes (internal stop)      :: 0 of 3
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3459
                     /organism="Methanocorpusculum sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51999"
                     /isolate="HAC023.59"
                     /isolation_source="fecal sample"
                     /db_xref="taxon:2058474"
                     /environmental_sample
                     /country="India"
                     /metagenome_source="feces metagenome"
                     /note="metagenomic"
     gene            <1..2238
                     /locus_tag="O0X37_05440"
     CDS             <1..2238
                     /locus_tag="O0X37_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011832991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed DNA polymerase"
                     /protein_id="HJK62399.1"
                     /translation="HSDTIEVTQDKAVSIKGEPLRRIYTGKPTDVRIVRDHYHHYEAD
                     IPFATRFLIDTGLTGGVTAPSEVCSYTELAPAVVHSKPRVCMCDIECDDRNGFPEPER
                     DPITCLTCHDSYDDHYTTFVLLGKTHVDYYEHSGLVNGCFIRDRHTICVYQTEKELFT
                     AFIAYIQEKDPDILSGWNFADFDADYILKRAQALGFKSDVFARIPGTTERNAMRGRVI
                     FDLLTAYKKMQGSQKESYRLDAIAEEELGERKVRYTGTLGDLWNNDPLKMVEYNFKDV
                     ELCVGINRKNNIIEFYQEVSRYVGCPLDKALNSSNVIDIYILRKAYGKFVLPSKGNTA
                     GEEFEGATVFAASKGVKENVIVLDLKSLYPMAMMTLNASPETKSPEGEIHAPNGIRFR
                     REPDGLTRSIISELMAERDDRKKLRNTFPYGSQEYTLYDMQQNVLKVIMNTYYGVSGF
                     SRFRLYDRDIGSAVTSVGRAIIQHTKNIITARGYEVIYGDTDSCFVQLPKVSQEETMK
                     IARELEAELNASYIDFSKDTLAADQNYFSIKFEKIYRRFFQGGAKKRYAGHLVWKEGQ
                     DVDKIDITGFEMKRSDSAPITREVQERVLEMILKGNGKAEVKEYLPEVLRMYRAGRCP
                     LEKAGIPSGINKSLDDYTVLDAHGRGAKYSNTYLGTDFKRGSKPKRLYIKRSYNPETY
                     PNTDVLCFEYPDQIPDGMFEIDWETMLEKTIQAPLTRIFDALGWDWDEFDPTKTKKTT
                     LDMFF"
     gene            2620..>3459
                     /locus_tag="O0X37_05445"
     CDS             2620..>3459
                     /locus_tag="O0X37_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004040350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HJK62400.1"
                     /translation="MVSDEIDAVLSQLENDNVRSVLLQFSDLEGKPKNVAIPTKQMKK
                     ALTDGISFDGSSIQGFARLEESDMLLRPDPATYQIIPWSDEKFRAARFICDVYTTRGE
                     PFAGDPRYILRTQINKAAESGYTFNVGPEMEFFLFRLVDGHASVNLQDHSGYFDQTPT
                     DPGEDVRRDLVISLSEMGFNIEASHHEVAPSQHEIDFTYGDALGMADKVVTFKFAAKT
                     LALKRGLHATFMPKPIFGINGSGMHVNCSLMKDGKNAFFEPEGDHQLSDTARYFIAGL
                     LKHI"
BASE COUNT          906 a          855 c          821 g          877 t
ORIGIN      
        1 cattcggata caattgaagt cacacaggac aaagcggttt ctatcaaggg agaaccgctt
       61 cgccgcatct acaccggaaa accaactgat gtaaggattg tgcgtgatca ctaccaccac
      121 tacgaagcag atattccttt tgccacccgt ttccttatcg acacaggcct caccggcggc
      181 gttactgcac cctctgaggt atgcagttac actgaacttg cacccgcagt tgttcactca
      241 aagccccgag tctgtatgtg cgatatcgag tgtgacgacc ggaacgggtt ccccgaacct
      301 gaacgtgatc cgataacctg tctcacctgt cacgactctt acgacgacca ttacacaaca
      361 tttgtcctcc tcggcaaaac acacgttgac tactatgaac actccggtct tgtgaacggc
      421 tgtttcatcc gcgaccgcca caccatctgt gtttatcaga cagaaaaaga actcttcact
      481 gcgttcatag cctacattca ggaaaaagat cccgatatcc tctccggctg gaactttgct
      541 gattttgatg ccgattatat ccttaagcgt gctcaggctc ttgggttcaa atcggatgtg
      601 tttgcacgca tcccgggaac aaccgaacgc aatgcaatgc gtggacgtgt catctttgat
      661 cttcttaccg cctacaaaaa gatgcaggga agccagaagg aatcctatcg gctggatgct
      721 atcgctgagg aagagcttgg tgaacgcaaa gtccgctata ccggaacact tggtgatctc
      781 tggaacaatg atcctctgaa aatggttgag tacaacttta aggatgtgga gttgtgcgtc
      841 gggatcaatc ggaaaaataa catcatcgag ttctatcagg aggtctcccg ttatgtcggt
      901 tgtcccctgg acaaagccct caactcctcc aacgttattg acatctacat tctccgcaaa
      961 gcatacggaa aattcgtcct cccgtccaaa ggaaacaccg ctggagaaga gtttgaagga
     1021 gcaacggtgt ttgctgcaag caaaggtgtc aaagaaaacg tgattgtgct tgatctcaaa
     1081 tccctctacc cgatggcgat gatgacgttg aatgcatccc cggagaccaa atcaccggag
     1141 ggtgagatcc atgcaccaaa tggcatccgc ttccgtcgtg agccagatgg tcttacccgc
     1201 agcatcatca gcgaacttat ggcagaacgt gacgatcgta aaaagctgcg caacaccttc
     1261 ccctatggtt ctcaggagta cacgctttac gacatgcagc agaatgtcct caaggtgatt
     1321 atgaatacgt actatggggt gtcgggtttc tctcggttcc ggctctatga tcgtgacatt
     1381 ggttctgctg tgacatcagt cggcagagcc atcatccagc acacgaaaaa tatcatcacc
     1441 gcccgtggtt atgaggtcat ttacggagat actgattcct gttttgtcca gttgccgaag
     1501 gtctcgcagg aagagacaat gaagattgca cgtgagcttg aagctgagtt gaatgcaagc
     1561 tacatcgatt tttccaaaga tacacttgcc gcagatcaga actacttctc catcaagttc
     1621 gaaaaaattt accgcagatt tttccagggt ggtgcaaaga aacggtatgc cggtcatctt
     1681 gtctggaaag agggacagga tgttgataag atcgacatca ccggctttga aatgaaacgt
     1741 tccgactcgg cacccatcac ccgcgaggta caggaacggg tgcttgagat gatcctcaaa
     1801 ggaaacggga aagccgaggt taaggagtat cttcctgaag tcctgcgcat gtatcgtgca
     1861 ggccgctgcc ctctggaaaa agccggaatc ccaagcggga tcaacaagtc acttgatgac
     1921 tacactgtac tcgatgccca tggccggggg gcaaaatact caaacacgta tcttggtact
     1981 gacttcaaac gaggcagtaa acctaaacgg ctctacatca aacggtctta caacccggaa
     2041 acctatccaa acacggatgt actctgtttt gagtatcctg atcagattcc tgacggaatg
     2101 tttgagatcg actgggagac tatgcttgaa aaaaccattc aggctccgtt aacaaggatc
     2161 tttgacgctc ttggatggga ttgggacgag tttgatccga ccaagacaaa gaaaaccaca
     2221 ctggatatgt tcttttgaaa acatggaaat ctgatttttc aggggagggg actccccgaa
     2281 tagtctgatt ttattgagat tctagaacga aaggcataga tgaatatgtg tgcgttttgg
     2341 aatcatagat gttctatctt gccattttgt ggaggattaa cggtttcatg tggtaagttc
     2401 tgtgttttgt atgatatcac ttccgggcag gggtgttgta ctgtctttct ctgcctaaga
     2461 gatacattcg tctctaaggt attccatctc ttgaatgtac tcaaaaactt cttcaggtct
     2521 gtaaaaaaat accagctact gcatcttcat acttcattca atgatccaga tatcccactg
     2581 agttcaggtt taatatcgta tcactgccca tatgtatgca tggtttctga cgaaatcgac
     2641 gctgttctct cacagcttga gaacgataat gtgcggtcgg ttcttcttca gttctcggat
     2701 ctggagggta agccgaaaaa cgtcgcaatt ccgacgaaac aaatgaaaaa ggcccttacc
     2761 gacggtatca gctttgacgg gtcatccatt caggggtttg cgagacttga ggagtcggat
     2821 atgctgctcc gccccgatcc agcaacatac cagatcattc cgtggtccga tgaaaagttc
     2881 cgggctgcac ggtttatctg cgatgtgtac accacacgcg gcgagccatt tgccggtgat
     2941 ccgcgctaca ttctgcgtac tcagatcaac aaggcggcgg agtccgggta cacctttaat
     3001 gtggggccgg agatggagtt cttcctcttc cgtctggtcg atggtcacgc atccgttaat
     3061 cttcaggatc acagtggtta ctttgatcag acaccgactg atcctggaga agatgtccgg
     3121 cgtgatttgg tcatctcgtt gtcagagatg gggttcaaca tcgaagcatc gcatcatgaa
     3181 gtcgctccaa gccagcacga gatcgacttt acctacggtg atgctctcgg catggctgat
     3241 aaagttgtga ccttcaagtt tgctgccaaa acccttgccc taaagcgtgg tctgcatgca
     3301 accttcatgc ccaaacccat cttcggcatc aacggttccg gtatgcacgt caactgctca
     3361 ttgatgaagg atggaaagaa tgcattcttc gaaccagaag gcgatcacca gctctccgac
     3421 actgcgcgtt actttatcgc aggtctcctg aaacacatc
//
